ca14 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ca14
Entrez Gene ID	567897
Full Name	carbonic anhydrase XIV
Synonyms	zgc:123188
General protein information	Preferred Names carbonic anhydrase XIV Names carbonic anhydrase 14
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328144.1	NP_001315073.1	carbonic anhydrase 14 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CA14	NP_036245.1
	CA14	XP_001099083.1
Bos taurus (cattle)	CA14	NP_001179134.1
Xenopus tropicalis (tropical clawed frog)	ca14	NP_001103521.1
Pan troglodytes (chimpanzee)	CA14	XP_524862.2
Mus musculus (house mouse)	Car14	NP_035927.1
Canis lupus familiaris (dog)	CA14	XP_003639672.2
Rattus norvegicus (Norway rat)	Car14	NP_001103125.1
Gallus gallus (chicken)	CA14	XP_003642713.2
Danio rerio (zebrafish)	ca14	NP_001032782.1

Gene Ontology
Bibliography

Related articles in PubMed

Carbonic Anhydrase Inhibitors Induce Developmental Toxicity During Zebrafish Embryogenesis, Especially in the Inner Ear.
Matsumoto H, Fujiwara S, Miyagi H, Nakamura N, Shiga Y, Ohta T, Tsuzuki M
Marine biotechnology (New York, N.Y.)19(5)430-440(2017 Oct)

Identification and characterization of a novel zebrafish (
Patrikainen MS, Tolvanen MEE, Aspatwar A, Barker HR, Ortutay C, J?nis J, Laitaoja M, Hyt?nen VP, Azizi L, Manandhar P, J?ger E, Vullo D, Kukkurainen S, Hilvo M, Supuran CT, Parkkila S
PeerJ5e4128(2017)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Chemokine-Dependent pH Elevation at the Cell Front Sustains Polarity in Directionally Migrating Zebrafish Germ Cells.
Tarbashevich K, Reichman-Fried M, Grimaldi C, Raz E
Current biology : CB25(8)1096-103(2015 Apr)

Close Association of Carbonic Anhydrase (CA2a and CA15a), Na(+)/H(+) Exchanger (Nhe3b), and Ammonia Transporter Rhcg1 in Zebrafish Ionocytes Responsible for Na(+) Uptake.
Ito Y, Kobayashi S, Nakamura N, Miyagi H, Esaki M, Hoshijima K, Hirose S
Frontiers in physiology459(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hypoxia-inducible carbonic anhydrase IX (CAIX) expression is insufficient to alleviate intracellular metabolic acidosis in the muscle of zebrafish, Danio rerio.
Title: Hypoxia-inducible carbonic anhydrase IX expression is insufficient to alleviate intracellular metabolic acidosis in the muscle of zebrafish, Danio rerio.

The following ca14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ca14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa45287	NM_001328144.1 Latest version!	Danio rerio carbonic anhydrase XIV (ca14), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ca14 ( NM_001328144.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315073.1 Danio rerio carbonic anhydrase XIV (ca14), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa45287
Clone ID Related Accession (Same CDS sequence)	NM_001328144.1
Accession Version	NM_001328144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	carbonic anhydrase 14 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU467889.10. On Jun 7, 2016 this sequence version replaced XM_009292074.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA3505381, SAMEA3505388 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGATCATA TCTGGACAAC CTTGGCTTTT TCTTTTGTTT TGAAGTGTGT GGAGTGCTCA 
GGAAGTGCAA CAAAATGGAC ATATACAGGT GCTGTGGGAC AACCAGAATG GATTGAGTTC 
TTTCCTGAAT GTGGTGGTTT CAATCAGTCA CCTGTAAATG TGGACACATC ACAAACACTA 
CATGACCCAA CACTAATTCC TGTGCAGCCC ATGGGATACA ACCAGCCTGG GAGAAGACCA 
TTCATCCTCT CTAATAATGG CCACACGGTG CAGATGACAT TGCCCCACTG GATGGGTGTA 
GGGGGGTTGC CATCACATTA TAGTGCTGTG CAGCTGCACC TGCACTGGGG TAATGGAGTG 
GGCATAGCAA CAGGAAGCGA ACACACTATT AATGGACAGA GCACTTCAGC AGAGCTTCAT 
ATTGTCCATT ACAATACAGA GGTTTATGCA AATCTGTCAG AAGCTATGAT GCAGAAGAAT 
GGCCTGGCTG TGTTAGGGAT CCTAATTGAG ACAGGTGAAG AGGTTAATCA GGCATATGGA 
AGTATTTTTA ACTATCTGGG ACGCATCAGA TATGCAGGAC AGAAGGTGGC TATCCCATCA 
TTTGATTTAC AGTCTCTGTT ACCTGAAAAT CTCAGCCAAT ATTTTCGCTA CAATGGCTCA 
CTCACCACCC CACCCTGCCA CGAGAGTGTC CTCTGGACCA TCTTCAATGA AAGGGTTAAA 
ATCTCTCACT CGCAGTTGTT AAAATTAGAG ACAGTATTGT ATTCCAGTAA GGCAGAAGAG 
GAAGAACCCA CCATTCTCCA GGATAACTAC CGTGCCACAC AACCACTTAA CCACAGAACT 
GTCCTGTCCT CTTTCATTCC TGTCTCAGTT CAAATCTATA CTGCTGGTGA AATTGCAGCA 
ATTGTGATTG GATCTCTGTT TGGATGTATT GGTTTGGCTG TTATTATCTG GTTCATTGTA 
AAAACTATTC GCTTAACATC CAGTTGGGAT GTCCTGCAAA GTATCCAACC TGCTCCTCAG 
CCCAGAATGC AAGATGCAAA CGAACACAAA CAGGACACGG ACTTAAAGAT GACCTCTAAT 
CAAGAAAAAA CAAAGGACAC TGAACATCAG CCTGAAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315073.1
CDS	154..1275
Translation	MDHIWTTLAFSFVLKCVECSGSATKWTYTGAVGQPEWIEFFPECGGFNQSPVNVDTSQTLHDPTLIPVQPMGYNQPGRRPFILSNNGHTVQMTLPHWMGVGGLPSHYSAVQLHLHWGNGVGIATGSEHTINGQSTSAELHIVHYNTEVYANLSEAMMQKNGLAVLGILIETGEEVNQAYGSIFNYLGRIRYAGQKVAIPSFDLQSLLPENLSQYFRYNGSLTTPPCHESVLWTIFNERVKISHSQLLKLETVLYSSKAEEEEPTILQDNYRATQPLNHRTVLSSFIPVSVQIYTAGEIAAIVIGSLFGCIGLAVIIWFIVKTIRLTSSWDVLQSIQPAPQPRMQDANEHKQDTDLKMTSNQEKTKDTEHQPET

Target ORF information:

RefSeq Version	NM_001328144.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbonic anhydrase XIV (ca14), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328144.1

ORF Insert Sequence:

ATGGATCATA TCTGGACAAC CTTGGCTTTT TCTTTTGTTT TGAAGTGTGT GGAGTGCTCA 
GGAAGTGCAA CAAAATGGAC ATATACAGGT GCTGTGGGAC AACCAGAATG GATTGAGTTC 
TTTCCTGAAT GTGGTGGTTT CAATCAGTCA CCTGTAAATG TGGACACATC ACAAACACTA 
CATGACCCAA CACTAATTCC TGTGCAGCCC ATGGGATACA ACCAGCCTGG GAGAAGACCA 
TTCATCCTCT CTAATAATGG CCACACGGTG CAGATGACAT TGCCCCACTG GATGGGTGTA 
GGGGGGTTGC CATCACATTA TAGTGCTGTG CAGCTGCACC TGCACTGGGG TAATGGAGTG 
GGCATAGCAA CAGGAAGCGA ACACACTATT AATGGACAGA GCACTTCAGC AGAGCTTCAT 
ATTGTCCATT ACAATACAGA GGTTTATGCA AATCTGTCAG AAGCTATGAT GCAGAAGAAT 
GGCCTGGCTG TGTTAGGGAT CCTAATTGAG ACAGGTGAAG AGGTTAATCA GGCATATGGA 
AGTATTTTTA ACTATCTGGG ACGCATCAGA TATGCAGGAC AGAAGGTGGC TATCCCATCA 
TTTGATTTAC AGTCTCTGTT ACCTGAAAAT CTCAGCCAAT ATTTTCGCTA CAATGGCTCA 
CTCACCACCC CACCCTGCCA CGAGAGTGTC CTCTGGACCA TCTTCAATGA AAGGGTTAAA 
ATCTCTCACT CGCAGTTGTT AAAATTAGAG ACAGTATTGT ATTCCAGTAA GGCAGAAGAG 
GAAGAACCCA CCATTCTCCA GGATAACTAC CGTGCCACAC AACCACTTAA CCACAGAACT 
GTCCTGTCCT CTTTCATTCC TGTCTCAGTT CAAATCTATA CTGCTGGTGA AATTGCAGCA 
ATTGTGATTG GATCTCTGTT TGGATGTATT GGTTTGGCTG TTATTATCTG GTTCATTGTA 
AAAACTATTC GCTTAACATC CAGTTGGGAT GTCCTGCAAA GTATCCAACC TGCTCCTCAG 
CCCAGAATGC AAGATGCAAA CGAACACAAA CAGGACACGG ACTTAAAGAT GACCTCTAAT 
CAAGAAAAAA CAAAGGACAC TGAACATCAG CCTGAAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Carbonic Anhydrase Inhibitors Induce Developmental Toxicity During Zebrafish Embryogenesis, Especially in the Inner Ear.
Marine biotechnology (New York, N.Y.)19(5)430-440(2017 Oct)
Matsumoto H,Fujiwara S,Miyagi H,Nakamura N,Shiga Y,Ohta T,Tsuzuki M

Identification and characterization of a novel zebrafish (
PeerJ5e4128(2017)
Patrikainen MS,Tolvanen MEE,Aspatwar A,Barker HR,Ortutay C,J?nis J,Laitaoja M,Hyt?nen VP,Azizi L,Manandhar P,J?ger E,Vullo D,Kukkurainen S,Hilvo M,Supuran CT,Parkkila S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Chemokine-Dependent pH Elevation at the Cell Front Sustains Polarity in Directionally Migrating Zebrafish Germ Cells.
Current biology : CB25(8)1096-103(2015 Apr)
Tarbashevich K,Reichman-Fried M,Grimaldi C,Raz E

Close Association of Carbonic Anhydrase (CA2a and CA15a), Na(+)/H(+) Exchanger (Nhe3b), and Ammonia Transporter Rhcg1 in Zebrafish Ionocytes Responsible for Na(+) Uptake.
Frontiers in physiology459(2013)
Ito Y,Kobayashi S,Nakamura N,Miyagi H,Esaki M,Hoshijima K,Hirose S

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ca14 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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