GCN1 cDNA ORF clone, Danio rerio(Zebrafish)

The following GCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ODa60004 XM_009303726.3
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Danio rerio GCN1 eIF2 alpha kinase activator homolog (gcn1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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ODa60004 XM_009303726.2 Danio rerio GCN1 eIF2 alpha kinase activator homolog (gcn1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa60004
    Clone ID Related Accession (Same CDS sequence) XM_009303726.3 , XM_009303726.2
    Accession Version XM_009303726.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eIF-2-alpha kinase activator GCN1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) and transcript sequence (GFIL01011289.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 84 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGG ACAGGTTTCA GACACACTGA AGAGATTCGC TGTGAAAGTC 
    ACGTCATCCA GTGTTAAAGA GCGCAAAGAA ATTCTTGGAG AACTGAAGGA ATGTGTGAAG
    GGAAAGGAGC TTCCAGAGCC GGCCATCAAA GGACTGTGCA GACTCTTCTA TCTCACGCTG
    CACAGATACA GGGATGCTGC GTCTCGGCGA GCATTACTGT CCGTCATCGA GGTGCTGGTT
    CAGTCTCAGC CTGATGTGGT GGCCAGTAAT CTGCCTTCAG GTCTGCTCAG CTGCGGTGTG
    GTCAGTAGAG GAGCGATGCC CGGGAAGAGT TCGGCGTCTG GAGCGTGCTG CGCTCTGCCC
    TGGACCTGTC TGATCGTCCG CTTCGTCTTT CCTACCGCAG ACAAGAGAGA AGGTGCCAAC
    TGGAAGAAAC TGGTGGAGGT TCAGAGTTTG CTGCTGGCAG AGGCAGTAGG CGGAGCCTCA
    GGGAACGCTC TCAAGTCAAT CAACAAACAC TTCAACAAGC TGTGGAAGGA GCACCCGGGT
    CTGGTGGATC AGTACATGAG CACTGTGTTG AGTCTGGATC AGAGTTGTGT GTTTGTTCCT
    CTGCTGGGGT TGTGTGTTGA TTTCTGCACA GCACAGAAAG ACATCGCCAC TGTCAACAAA
    CACAAGGCAG CTCTCCTGGA TCTCTACATG AAGACGGTGC TGATGAGTAA AACCAGACCA
    CTTCAGCACA TACTGGAAAA GAGCGGCTCT ATTCTTCGCC ACATGTCTCA CGCTGAGTTT
    AAGGAGCAGC TGCTGCCCAC ATTACAGAAA GCTCTTCTGC GCAGCCCTGA GAACTGCATG
    CAGACAATCT CCTCCATGTT GGCGTCTCTC ACTCTGGATC TGAGTCAGTA CGCGCTGGAC
    ATCGGAAAGG GCATCTCCAG TCAGCTGAAG GCTAATAACC CAGAGCTGAT GGAGCAGGCG
    GTGGCGGCGC TGCAGAATCT GACCCAGCAG TGCAGTGACC CAAGCGCCGT CCAGGACCTC
    GCCAAACACC TCTTCAACAT CCTCGCAGGC TCAGAGGGGA AGCTGACTGT TGTGGCCCAG
    AAGATGAGCG TCCTCTCAGG AATCAGGAGC TGTAGTCATC ACGCTGTCTC TGGCTCTTCT
    TCTCAGTCTC TGAGCTCCAG CGTGGCTGTG ATGTTCATCC CATACCTGCA GCAGGAAGTC
    CACGAGGGGA CGCTGGTCCA TGCAGTGTCG GTGTTGTCTC AGTGGACGAG CCGCCTGACG
    CTGGATGTGC CCTCAGAGTT GAGAGACTGG TTTAAAAAAG CCTTCACCCT GAAGACGTCC
    ACATCCGCAG TGCGACACGC GTATCTGCAG GCCATGATCG GTGCTTTTAA AGGTGACTCT
    CTCAGCCAGG CTGTAGACTT CCTGCCTCTG CTCATTCAGA CGGTGGAGAA AGCTGCAGCT
    CAGAATACCC AGCATACACT GCTGTCTGAG GCCGTGGCTG CATCTGTGCT GCTCTGTCGG
    CTCTCTGTGC TGGACTCTGT GTCTGAAGCG AAGCTGACGT CCTTCTGGAA CCTGATCCTG
    GATGAGAAGA AGCCTTTGTT CACCACTGAG AAGTTCCTCT CTCAGGCCAG TGAAGAAGCC
    CTTTGCACCG TTCTACTGCT CTGTGAAAGA CTGTTCCTGG ATCAGCCACA AAGACTCAAC
    AACAGCAAGT CTCAGATGTA CCATCAGGCC ACAGTGATGC CGCTGCTGTC CCGTCTGTGG
    CCCGTACGCA AACGCGCTCA GCAAACAGTG AAGAAGCTCC TGTCCTCGCT GGGTGGATCC
    AGTCTGGCCC ACGGCCTTCT GGCAGAACTG CGTGTAGTTA TTAACAAACA CAAGATTCTG
    CCCGCAGACG TGCTGTATAC AGAGACGGGT GAGCTGACTG AGACGGGCCG CTCGTATGTG
    TCTCCGCGGG TCCTGCAGGA GGCTTTGAGT GTCATCTGCT CAGTTGCGGC CCAGTGGAAC
    GCCCCGGATG AGACAGAGAA ACTGGCCCTG GAGATTCTCA TCGTTACCCA TCATCCCTCT
    ATCGTGGCTG TCCGGTCTGA GCTGTGGTCT TCTCTGCTGT CTGCTATGAA GCTGGACGCT
    GCTCAATTCA TCGACAAACA CCTGGAAGTG ATTCTACCTC GCCTGCTGGA AGCCAACACA
    GACAATCAGG CTGTTCAGAA TGCTGTCGGT TCTCTGTCTC TGCTGTCTCC CAACAAACTG
    CTGCCGCGGG TCATGGATCA CGTGACCCAG CGATTGTCCA ATCCTGCACT GAGTCTGGTG
    ACGCGTCAGG AATACGCAAT CATGCAAACT CCAGAGGGAG AACTTTACGA CAAGAGCATC
    ATTATGAGTG CACAGCAGGA GAGCACAAAG AAAGGCAACA TGAAGAGAGA GAATAAAGCG
    TATTCCTTCA AAGAGCAGAT CATTGAACTG GAGCTTCAGG AGGAGATCAA GAAGAAGAAG
    GGCATCAAAG AGGAGATCCA GCTGACTAGC AAACAGAAGG AGATGATGCA GATCCAGCTG
    GAGAAAGAGT CTACTATCCG CAAGCGTCTG CAGGAGCTGG ACATGGAGCT GCTGTGTGCT
    GTGGGTTTGC TGGAAGCCGC TCTGGCTCGT CGTCCTCCGC TCATCTGGAT GCACCTTCCA
    GCACTGGTGC AGGTTCTCCT GCCGCTCCTG CAGTCTCCTC TGGCCGCTTC GTTGATAAAG
    CGTGTGTTTC TGCAGATCGG TGTCACTCTA ATGCCTAAAG AACTGCACCA GCTGGCGGTT
    CTGGTGGGTC ATGTGACCTT GCGTCTGCTG AAGCCAGAGT GTGATTTGGA TCCAGCCTGG
    GCCCAAGAAG ACCTGAACAC AGCGACACAG CGAACCATCT CACTGCTGCA CCAAAGAGAC
    GGCAAAACAG GATCAGATGT GCTGGTGTTG TCGGCTCCAG CGTTCTCCTT CTGTTTCCCG
    CTGCTGCAGG CTGTGTTGAG TCAGAGCAGC GGATCCTCAG AGGAGTCTGA GCTCATGATG
    ACGCGAGCGC TGCAGGTCAT CAACACACAC GCACAGCTGC GGGCGCAGGA CGACACCACC
    GACACCTTCA TAGATGAGAA TGGCCCAGAG CTGCTGCCGA GGGTCAGTAT GCTGCAGCTG
    CTGACCAAAG TGATCGGGAC GTCCACTCCT CGCCTTCAGG TTCTGGCGTC CAGCTGTCTG
    ACAGCTCTGT GTGGCAGTGC TGGAGGTCAG GAGGGCTGTG CTCTCGCTGA ACAACCTGAG
    ATCGATGTTC TGCTGGAGGC TTTACTGTCC TCCTGTTTCT CCGTCAGAGA CGCTGCGCTC
    CGGGGTCTGC TGGAGATGGA GCTGGCGTTA CCCACTGACA GCACTGATGT CAATGGCTTG
    AAGATGTTGA GGAGGCTCTG GGTGGCCAAG TTTGATGTGG AGGAGGAGGC CAGGGCTCTG
    GCGGAGAAAC TGTGGCAGGC TCTGTGTCTG GATCTGGTGC CGGAGCTGTG TCCACTGCTG
    ATTGAGGATG TGATCCAGCA TGAGGAGGCG GTGCGTTCAG CCGGTGCGGA GGCTTTGTCC
    AGCGCCGTCA GTCAGTATCA GGAGCAGTCA GCGACTGTCC TCTCACAGCT CACTCAACTC
    TACCATCAGA AGCTATATAG GCCGCCTCCA GTTCTGGATG CTCTTGGACG CGTTATTTCT
    GAAGCACCTC CTGATCAATG GGAGGCCAGG TGTGGCATTG CTCTTGCGCT GAACAAACTG
    GCTGAGTACC TGGACGAGAG TCAGGTGACG CCACTCTTCC TGTTCTTTGT GCCGGACGCT
    CTGAATGACA GACACCCTGA GGTGCGGCGC TGCATGCTGG ACGCGGCACT GTCTGCTCTT
    AACACACACG GCAAGGATAA CGTGAGCTCT CTGCTGCCCG TGTTTGAGGA GTTTCTGAAG
    AACGCACCGC AGGACGCCAG CTATGACTCT GTGCGGCAGA GTGTCGTCAT CCTCATGGGC
    TCGCTGGCCA AACATCTGGA CAAGAGCGAC CCCAAAGTCA AACCCATCGT GGCCAAGCTG
    ATCACAGCCC TTTCCACTCC CTCACAGCAG GTTCAGGAGT CGGTGGCCAG TTGCCTTCCT
    CCTCTGGTTC CTGCTATAAA GGAGGATGCA GGAGGGATGG TGCGGAAACT CCTGCAGCTG
    CTTCTGGAGA GCGACAAGTA CGCCGAACGC AAAGGAGCCG CATACGGACT GGCCGGACTG
    GTCAAAGGTC TGGGCATCTT ATCACTCAAA CAGCAGGAGA TCATGACCAC CCTGACGGAC
    GCCATTCAGG ACAAGAAGAA CTCCAGACGC AGAGAAGGGG CTCTGTTTGC GTTTGAGATG
    TTGTGTAACA TGCTGGGGAA GTTATTCGAG CCATATGTGG TTCATGTCCT CCCTCACTTG
    CTGCTGTGTT TCGGTGATGG GAATCAGTAC GTCAGAGAGG CTGCAGATGA CTGTGCTAAG
    GCTGTGATGA GGAATCTGAG CGCTCACGGG GTGAAGCTGG TTCTGCCGTC TCTGCTGGTG
    GCGCTGGAGG AGGAGTCCTG GAGAACCAAA GCAGGCTCAG TGGAGTTGTT GGGTGCGATG
    GCGTACTGCG CTCCTAAACA GCTGTCCTCA TGTCTGCCCA ACATTGTCCC GAAGCTGACA
    GAGGTTCTGA CAGACTCTCA TGTGAAGGTG CAGAAGGCCG GACAGCAGGC GCTTCGACAG
    ATCGGCTCCG TCATCCGCAA CCCTGAGATC CTCGCGATCA CCCCGATCCT GCTGGACGCT
    CTGACTGACC CGTCTCACAG GACGCAGCAC TGCCTGCAGA CACTGCTGGA CACCAAGTTT
    GTGCATTTCA TCGATGCTCC ATCTCTGGCT CTGATCATGC CCATCGTCCA GAGAGCCTTC
    CAGGACAGAT CCACCGACAC GCGCAAGATG GCGGCGCAGA TCATCGGAAA CATGTACTCA
    CTCACTGACC AGAAGGATCT GTCTCCGTAT TTGCCCAGTG TGATTCCAGG CCTCAAAGCA
    TCTCTGCTGG ACCCTGTGCC AGAGGTGCGG ACGGTGTCAG CGAAGGCTCT GGGTGCGATG
    GTGAAGGGAA TGGGTGAGTC CTCCTTTGAG GATCTGCTGC CGTGGCTGAT GGAGACTCTG
    GCGTCTGAAC AGAGCTCTGT GGATCGATCA GGAGCTGCGC AAGGTCTGGC TGAGGTGATG
    GCGGCTCTAG GTGTGGAGAA GCTGGATAAA CTGATGCCAG ATGTGGTTCA GACAGCCAGT
    AAAGTGGACA TCGCCTCTCA CGTCCGAGAC GGATATATCA TGATGTTTAT CTACCTTCCT
    CTGACCTTCG GGGACAAGTT CACCCCATAT GTGGGTCCCA TCATCCCCTG CATCCTAAAG
    GCTCTGGCTG ATGAGAACGA GTATGTTCGA GACACAGCTC TGCGTGCGGG TCAGAGGATC
    ATCAGCATGT ACGCAGAGAC GGCCATCGCT CTACTGCTGC CCGAGCTGGA GCAGGGCCTG
    TTTGATGACC TCTGGAGGAT CAGATTCAGC TCTGTGCAGT TGCTAGGAGA CCTGCTGTTC
    CATATCTCGG GGGTAACAGG AAAGATGACA ACAGAAACAG CATCAGAGGA TGACAACTTT
    GGCACGGCCC AGTCAAATAA GGCCATTATC TCAGCTCTGG GTGCAGAGAG GCGTAACCGT
    GTGCTGTCTG GACTCTACAT GGGCCGCTCA GACACACAGC TGGTGGTCCG ACAGGCGTCT
    CTTCATGTGT GGAAGATTGT GGTGTCAAAC ACTCCTCGAA CCCTCAGAGA AATCCTGCCC
    ACACTCTTCA CGCTGCTGCT GGGCTTCCTG GCCTCCACCT GCCCCGACAA GAGAACGATC
    GCCGCTCGAA CCCTGGGTGA CCTGGTGAGA AAGCTGGGAG AGAAGATCCT GCCTGAGATC
    ATTCCCATCC TGGAGGACGG TCTGCGTTCA GACAAGAGTG ACGAGCGCCA GGGTGTCTGC
    ATCGGCCTTA GCGAGATCAT GAAGTCCACC AGCAAAGACG CTGTTCTGGT GTTCTCCGAG
    TCTCTGGTGC CCACCGTGCG CAAGGCTCTG TGTGACCCGC TGGAGGAGGT TCGAGAAGCG
    GCTGCTAAAA CATTCGAGCA GCTTCACACC ACCATCGGTC ACCAGGCCCT GGACGACATC
    CTGCCCGCAC TGCTCAAACA ACTGGAGGAC GAGGAGACGT CAGAGTTTGC TCTGGATGGA
    CTGAAGCAGG TCATGGCTGT GAAGAGTCGC TCAGTCCTGC CGTACCTGGT TCCCAAGCTG
    ACGGCTCCTC CGGTGAACAC GCGTGTGCTG GCGTTTCTGT CTGCGGTGGC GGGAGATGCT
    CTGACGCGGC ACCTCGGCGT CATCCTGCCC GCTCTCCTCT CCTCTCTCAA AGACAAGCTG
    GGCACAGAGG AGAGTCTGCA GGAACTGAGC AACTGTCAGA CGGTGATTCT GTCAGTGGAG
    GATGAAGTGG GTCAGAGGAT CATCATTGAA GATCTGCTGG AGGCGACGCG AGGAGCGGAC
    GCTGGGCTCA GACAGGCCTC AATCACCATC CTCAACGGCT ATTTCTCCCG CACACGGCTG
    GACTACAGCG CTCACACACG CACACTGCTG TCAGGACTCA TGCGTCTCAT GAATGACCCC
    AACACTGAGG TGCTGAGCCA GAGCTGGGAC ACCATCAACT CCATTACAAA GAAACTGGAT
    GCGGGCAGTC AGCTCTCTCT GATTGATGAC CTGCACAGGG ACATCCGCTC TGCTGCTGCT
    GAGGTCAAAG GTCAACACTT ACCTGGCTTC TGCCTGCCCA AGAAGGGAGT GACCTGCATT
    CTCCCTGTGC TGAGGGAAGG TGTGCTGACA GGAAGTCCAG AGCAGAAAGA GGAAGCGGCT
    CGAGCTTTAG GTGGAGTCAT TAAACTGACC TCAGCGGAGG CTCTGCGGCC GTCAGTGGTC
    AACATCACTG GACCGCTCAT CCGAATCCTG GGAGACCGAT TCGCATGGAA CGTCAAAACT
    GCTCTTCTGG AGACCCTTAC ACTACTGCTG GCCAAAGTGG GCATCGCTCT GAAGCCCTTC
    CTGCCGCAGC TGCAGACCAC CTTCCTAAAG GCGTTGCAGG ACTCCAGTCG GGCTGTGAGG
    CTGCGGGCGG CGGAGGCTCT GGGGCAGCTC GTTTCCATCC ACACCAAAGT AGATCCACTG
    TTCACTGAGC AGCTGGCAGC CATACGCAAC GCTGAGGACT CTGGAGTCAG GGAGACGATG
    TTGCAGGCCC TGCGATTTGT CATTCAGGGA GCAGGAGCCA AGGTGGACCC CGCCATCAGG
    AAGAACATCA CCACCACACT GCTGAGCATG CTGGGACATG ATGAGGATGC CACAAGAATG
    GCCTCTGCGG GCTGTATTGG TGAACTCTGT GCCTTTCTAT CAGAAGAGGA GCTGAAGAGT
    GTCTTACAGC AGCACATCCT CGCGGATGTG TCTGGCGTGG ACTGGATGGT TCGTCATGGG
    CGTAGTTTGG CTCTGTCTAT CGCCGTGAAG TCTGCTCCTG CTCAGCTGTG TGTGGATGAG
    TACAACTCCT CTGTGCTGGA GGCCGTCTTG TCCAGCGCCA CCGCTGACAG AATCCCCATC
    GCCTGCAGTG GAATCAGAGC CATGGGTTTC CTGATGAGAC ACCAGCTCCG CACTGGAGGC
    TCAGAGAGTG TATCGCCCCG CATCATCACA CAGTTCATCA AGTGTCTCCA AAACCAGTCG
    AGTGACATTC GTCTGGTGGC AGAGCGGGTG TTGTGGTGGG TCTGGAAGGA GGACGAGACC
    CCCATTCTGG AGCCCAGTCT GGTCAAACCG CTCATCAAAG CACTGCTGGA CAACACCAAA
    GACAAGAACA CCAGCGTCCG AGCGCAGAGC GAACACACAC TCGTCAGCCT GCTGAGACTG
    CGGCGCGGAG AACACAACAT GCAGAGCATC AGTGCCATCC TGGACTCGGC CAGTAATGAC
    CTGCTGTCCG AATGTTACCG CAGATCCTTG AAGAAGATCT CCAGCCTTCC AGACTCCAAT
    GAAGAGATCG ATGATACCAT CCTCACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302001.2
    CDS67..8076
    Translation

    Target ORF information:

    RefSeq Version XM_009303726.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GCN1 eIF2 alpha kinase activator homolog (gcn1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303726.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGG ACAGGTTTCA GACACACTGA AGAGATTCGC TGTGAAAGTC 
    ACGTCATCCA GTGTTAAAGA GCGCAAAGAA ATTCTTGGAG AACTGAAGGA ATGTGTGAAG
    GGAAAGGAGC TTCCAGAGCC GGCCATCAAA GGACTGTGCA GACTCTTCTA TCTCACGCTG
    CACAGATACA GGGATGCTGC GTCTCGGCGA GCATTACTGT CCGTCATCGA GGTGCTGGTT
    CAGTCTCAGC CTGATGTGGT GGCCAGTAAT CTGCCTTCAG GTCTGCTCAG CTGCGGTGTG
    GTCAGTAGAG GAGCGATGCC CGGGAAGAGT TCGGCGTCTG GAGCGTGCTG CGCTCTGCCC
    TGGACCTGTC TGATCGTCCG CTTCGTCTTT CCTACCGCAG ACAAGAGAGA AGGTGCCAAC
    TGGAAGAAAC TGGTGGAGGT TCAGAGTTTG CTGCTGGCAG AGGCAGTAGG CGGAGCCTCA
    GGGAACGCTC TCAAGTCAAT CAACAAACAC TTCAACAAGC TGTGGAAGGA GCACCCGGGT
    CTGGTGGATC AGTACATGAG CACTGTGTTG AGTCTGGATC AGAGTTGTGT GTTTGTTCCT
    CTGCTGGGGT TGTGTGTTGA TTTCTGCACA GCACAGAAAG ACATCGCCAC TGTCAACAAA
    CACAAGGCAG CTCTCCTGGA TCTCTACATG AAGACGGTGC TGATGAGTAA AACCAGACCA
    CTTCAGCACA TACTGGAAAA GAGCGGCTCT ATTCTTCGCC ACATGTCTCA CGCTGAGTTT
    AAGGAGCAGC TGCTGCCCAC ATTACAGAAA GCTCTTCTGC GCAGCCCTGA GAACTGCATG
    CAGACAATCT CCTCCATGTT GGCGTCTCTC ACTCTGGATC TGAGTCAGTA CGCGCTGGAC
    ATCGGAAAGG GCATCTCCAG TCAGCTGAAG GCTAATAACC CAGAGCTGAT GGAGCAGGCG
    GTGGCGGCGC TGCAGAATCT GACCCAGCAG TGCAGTGACC CAAGCGCCGT CCAGGACCTC
    GCCAAACACC TCTTCAACAT CCTCGCAGGC TCAGAGGGGA AGCTGACTGT TGTGGCCCAG
    AAGATGAGCG TCCTCTCAGG AATCAGGAGC TGTAGTCATC ACGCTGTCTC TGGCTCTTCT
    TCTCAGTCTC TGAGCTCCAG CGTGGCTGTG ATGTTCATCC CATACCTGCA GCAGGAAGTC
    CACGAGGGGA CGCTGGTCCA TGCAGTGTCG GTGTTGTCTC AGTGGACGAG CCGCCTGACG
    CTGGATGTGC CCTCAGAGTT GAGAGACTGG TTTAAAAAAG CCTTCACCCT GAAGACGTCC
    ACATCCGCAG TGCGACACGC GTATCTGCAG GCCATGATCG GTGCTTTTAA AGGTGACTCT
    CTCAGCCAGG CTGTAGACTT CCTGCCTCTG CTCATTCAGA CGGTGGAGAA AGCTGCAGCT
    CAGAATACCC AGCATACACT GCTGTCTGAG GCCGTGGCTG CATCTGTGCT GCTCTGTCGG
    CTCTCTGTGC TGGACTCTGT GTCTGAAGCG AAGCTGACGT CCTTCTGGAA CCTGATCCTG
    GATGAGAAGA AGCCTTTGTT CACCACTGAG AAGTTCCTCT CTCAGGCCAG TGAAGAAGCC
    CTTTGCACCG TTCTACTGCT CTGTGAAAGA CTGTTCCTGG ATCAGCCACA AAGACTCAAC
    AACAGCAAGT CTCAGATGTA CCATCAGGCC ACAGTGATGC CGCTGCTGTC CCGTCTGTGG
    CCCGTACGCA AACGCGCTCA GCAAACAGTG AAGAAGCTCC TGTCCTCGCT GGGTGGATCC
    AGTCTGGCCC ACGGCCTTCT GGCAGAACTG CGTGTAGTTA TTAACAAACA CAAGATTCTG
    CCCGCAGACG TGCTGTATAC AGAGACGGGT GAGCTGACTG AGACGGGCCG CTCGTATGTG
    TCTCCGCGGG TCCTGCAGGA GGCTTTGAGT GTCATCTGCT CAGTTGCGGC CCAGTGGAAC
    GCCCCGGATG AGACAGAGAA ACTGGCCCTG GAGATTCTCA TCGTTACCCA TCATCCCTCT
    ATCGTGGCTG TCCGGTCTGA GCTGTGGTCT TCTCTGCTGT CTGCTATGAA GCTGGACGCT
    GCTCAATTCA TCGACAAACA CCTGGAAGTG ATTCTACCTC GCCTGCTGGA AGCCAACACA
    GACAATCAGG CTGTTCAGAA TGCTGTCGGT TCTCTGTCTC TGCTGTCTCC CAACAAACTG
    CTGCCGCGGG TCATGGATCA CGTGACCCAG CGATTGTCCA ATCCTGCACT GAGTCTGGTG
    ACGCGTCAGG AATACGCAAT CATGCAAACT CCAGAGGGAG AACTTTACGA CAAGAGCATC
    ATTATGAGTG CACAGCAGGA GAGCACAAAG AAAGGCAACA TGAAGAGAGA GAATAAAGCG
    TATTCCTTCA AAGAGCAGAT CATTGAACTG GAGCTTCAGG AGGAGATCAA GAAGAAGAAG
    GGCATCAAAG AGGAGATCCA GCTGACTAGC AAACAGAAGG AGATGATGCA GATCCAGCTG
    GAGAAAGAGT CTACTATCCG CAAGCGTCTG CAGGAGCTGG ACATGGAGCT GCTGTGTGCT
    GTGGGTTTGC TGGAAGCCGC TCTGGCTCGT CGTCCTCCGC TCATCTGGAT GCACCTTCCA
    GCACTGGTGC AGGTTCTCCT GCCGCTCCTG CAGTCTCCTC TGGCCGCTTC GTTGATAAAG
    CGTGTGTTTC TGCAGATCGG TGTCACTCTA ATGCCTAAAG AACTGCACCA GCTGGCGGTT
    CTGGTGGGTC ATGTGACCTT GCGTCTGCTG AAGCCAGAGT GTGATTTGGA TCCAGCCTGG
    GCCCAAGAAG ACCTGAACAC AGCGACACAG CGAACCATCT CACTGCTGCA CCAAAGAGAC
    GGCAAAACAG GATCAGATGT GCTGGTGTTG TCGGCTCCAG CGTTCTCCTT CTGTTTCCCG
    CTGCTGCAGG CTGTGTTGAG TCAGAGCAGC GGATCCTCAG AGGAGTCTGA GCTCATGATG
    ACGCGAGCGC TGCAGGTCAT CAACACACAC GCACAGCTGC GGGCGCAGGA CGACACCACC
    GACACCTTCA TAGATGAGAA TGGCCCAGAG CTGCTGCCGA GGGTCAGTAT GCTGCAGCTG
    CTGACCAAAG TGATCGGGAC GTCCACTCCT CGCCTTCAGG TTCTGGCGTC CAGCTGTCTG
    ACAGCTCTGT GTGGCAGTGC TGGAGGTCAG GAGGGCTGTG CTCTCGCTGA ACAACCTGAG
    ATCGATGTTC TGCTGGAGGC TTTACTGTCC TCCTGTTTCT CCGTCAGAGA CGCTGCGCTC
    CGGGGTCTGC TGGAGATGGA GCTGGCGTTA CCCACTGACA GCACTGATGT CAATGGCTTG
    AAGATGTTGA GGAGGCTCTG GGTGGCCAAG TTTGATGTGG AGGAGGAGGC CAGGGCTCTG
    GCGGAGAAAC TGTGGCAGGC TCTGTGTCTG GATCTGGTGC CGGAGCTGTG TCCACTGCTG
    ATTGAGGATG TGATCCAGCA TGAGGAGGCG GTGCGTTCAG CCGGTGCGGA GGCTTTGTCC
    AGCGCCGTCA GTCAGTATCA GGAGCAGTCA GCGACTGTCC TCTCACAGCT CACTCAACTC
    TACCATCAGA AGCTATATAG GCCGCCTCCA GTTCTGGATG CTCTTGGACG CGTTATTTCT
    GAAGCACCTC CTGATCAATG GGAGGCCAGG TGTGGCATTG CTCTTGCGCT GAACAAACTG
    GCTGAGTACC TGGACGAGAG TCAGGTGACG CCACTCTTCC TGTTCTTTGT GCCGGACGCT
    CTGAATGACA GACACCCTGA GGTGCGGCGC TGCATGCTGG ACGCGGCACT GTCTGCTCTT
    AACACACACG GCAAGGATAA CGTGAGCTCT CTGCTGCCCG TGTTTGAGGA GTTTCTGAAG
    AACGCACCGC AGGACGCCAG CTATGACTCT GTGCGGCAGA GTGTCGTCAT CCTCATGGGC
    TCGCTGGCCA AACATCTGGA CAAGAGCGAC CCCAAAGTCA AACCCATCGT GGCCAAGCTG
    ATCACAGCCC TTTCCACTCC CTCACAGCAG GTTCAGGAGT CGGTGGCCAG TTGCCTTCCT
    CCTCTGGTTC CTGCTATAAA GGAGGATGCA GGAGGGATGG TGCGGAAACT CCTGCAGCTG
    CTTCTGGAGA GCGACAAGTA CGCCGAACGC AAAGGAGCCG CATACGGACT GGCCGGACTG
    GTCAAAGGTC TGGGCATCTT ATCACTCAAA CAGCAGGAGA TCATGACCAC CCTGACGGAC
    GCCATTCAGG ACAAGAAGAA CTCCAGACGC AGAGAAGGGG CTCTGTTTGC GTTTGAGATG
    TTGTGTAACA TGCTGGGGAA GTTATTCGAG CCATATGTGG TTCATGTCCT CCCTCACTTG
    CTGCTGTGTT TCGGTGATGG GAATCAGTAC GTCAGAGAGG CTGCAGATGA CTGTGCTAAG
    GCTGTGATGA GGAATCTGAG CGCTCACGGG GTGAAGCTGG TTCTGCCGTC TCTGCTGGTG
    GCGCTGGAGG AGGAGTCCTG GAGAACCAAA GCAGGCTCAG TGGAGTTGTT GGGTGCGATG
    GCGTACTGCG CTCCTAAACA GCTGTCCTCA TGTCTGCCCA ACATTGTCCC GAAGCTGACA
    GAGGTTCTGA CAGACTCTCA TGTGAAGGTG CAGAAGGCCG GACAGCAGGC GCTTCGACAG
    ATCGGCTCCG TCATCCGCAA CCCTGAGATC CTCGCGATCA CCCCGATCCT GCTGGACGCT
    CTGACTGACC CGTCTCACAG GACGCAGCAC TGCCTGCAGA CACTGCTGGA CACCAAGTTT
    GTGCATTTCA TCGATGCTCC ATCTCTGGCT CTGATCATGC CCATCGTCCA GAGAGCCTTC
    CAGGACAGAT CCACCGACAC GCGCAAGATG GCGGCGCAGA TCATCGGAAA CATGTACTCA
    CTCACTGACC AGAAGGATCT GTCTCCGTAT TTGCCCAGTG TGATTCCAGG CCTCAAAGCA
    TCTCTGCTGG ACCCTGTGCC AGAGGTGCGG ACGGTGTCAG CGAAGGCTCT GGGTGCGATG
    GTGAAGGGAA TGGGTGAGTC CTCCTTTGAG GATCTGCTGC CGTGGCTGAT GGAGACTCTG
    GCGTCTGAAC AGAGCTCTGT GGATCGATCA GGAGCTGCGC AAGGTCTGGC TGAGGTGATG
    GCGGCTCTAG GTGTGGAGAA GCTGGATAAA CTGATGCCAG ATGTGGTTCA GACAGCCAGT
    AAAGTGGACA TCGCCTCTCA CGTCCGAGAC GGATATATCA TGATGTTTAT CTACCTTCCT
    CTGACCTTCG GGGACAAGTT CACCCCATAT GTGGGTCCCA TCATCCCCTG CATCCTAAAG
    GCTCTGGCTG ATGAGAACGA GTATGTTCGA GACACAGCTC TGCGTGCGGG TCAGAGGATC
    ATCAGCATGT ACGCAGAGAC GGCCATCGCT CTACTGCTGC CCGAGCTGGA GCAGGGCCTG
    TTTGATGACC TCTGGAGGAT CAGATTCAGC TCTGTGCAGT TGCTAGGAGA CCTGCTGTTC
    CATATCTCGG GGGTAACAGG AAAGATGACA ACAGAAACAG CATCAGAGGA TGACAACTTT
    GGCACGGCCC AGTCAAATAA GGCCATTATC TCAGCTCTGG GTGCAGAGAG GCGTAACCGT
    GTGCTGTCTG GACTCTACAT GGGCCGCTCA GACACACAGC TGGTGGTCCG ACAGGCGTCT
    CTTCATGTGT GGAAGATTGT GGTGTCAAAC ACTCCTCGAA CCCTCAGAGA AATCCTGCCC
    ACACTCTTCA CGCTGCTGCT GGGCTTCCTG GCCTCCACCT GCCCCGACAA GAGAACGATC
    GCCGCTCGAA CCCTGGGTGA CCTGGTGAGA AAGCTGGGAG AGAAGATCCT GCCTGAGATC
    ATTCCCATCC TGGAGGACGG TCTGCGTTCA GACAAGAGTG ACGAGCGCCA GGGTGTCTGC
    ATCGGCCTTA GCGAGATCAT GAAGTCCACC AGCAAAGACG CTGTTCTGGT GTTCTCCGAG
    TCTCTGGTGC CCACCGTGCG CAAGGCTCTG TGTGACCCGC TGGAGGAGGT TCGAGAAGCG
    GCTGCTAAAA CATTCGAGCA GCTTCACACC ACCATCGGTC ACCAGGCCCT GGACGACATC
    CTGCCCGCAC TGCTCAAACA ACTGGAGGAC GAGGAGACGT CAGAGTTTGC TCTGGATGGA
    CTGAAGCAGG TCATGGCTGT GAAGAGTCGC TCAGTCCTGC CGTACCTGGT TCCCAAGCTG
    ACGGCTCCTC CGGTGAACAC GCGTGTGCTG GCGTTTCTGT CTGCGGTGGC GGGAGATGCT
    CTGACGCGGC ACCTCGGCGT CATCCTGCCC GCTCTCCTCT CCTCTCTCAA AGACAAGCTG
    GGCACAGAGG AGAGTCTGCA GGAACTGAGC AACTGTCAGA CGGTGATTCT GTCAGTGGAG
    GATGAAGTGG GTCAGAGGAT CATCATTGAA GATCTGCTGG AGGCGACGCG AGGAGCGGAC
    GCTGGGCTCA GACAGGCCTC AATCACCATC CTCAACGGCT ATTTCTCCCG CACACGGCTG
    GACTACAGCG CTCACACACG CACACTGCTG TCAGGACTCA TGCGTCTCAT GAATGACCCC
    AACACTGAGG TGCTGAGCCA GAGCTGGGAC ACCATCAACT CCATTACAAA GAAACTGGAT
    GCGGGCAGTC AGCTCTCTCT GATTGATGAC CTGCACAGGG ACATCCGCTC TGCTGCTGCT
    GAGGTCAAAG GTCAACACTT ACCTGGCTTC TGCCTGCCCA AGAAGGGAGT GACCTGCATT
    CTCCCTGTGC TGAGGGAAGG TGTGCTGACA GGAAGTCCAG AGCAGAAAGA GGAAGCGGCT
    CGAGCTTTAG GTGGAGTCAT TAAACTGACC TCAGCGGAGG CTCTGCGGCC GTCAGTGGTC
    AACATCACTG GACCGCTCAT CCGAATCCTG GGAGACCGAT TCGCATGGAA CGTCAAAACT
    GCTCTTCTGG AGACCCTTAC ACTACTGCTG GCCAAAGTGG GCATCGCTCT GAAGCCCTTC
    CTGCCGCAGC TGCAGACCAC CTTCCTAAAG GCGTTGCAGG ACTCCAGTCG GGCTGTGAGG
    CTGCGGGCGG CGGAGGCTCT GGGGCAGCTC GTTTCCATCC ACACCAAAGT AGATCCACTG
    TTCACTGAGC AGCTGGCAGC CATACGCAAC GCTGAGGACT CTGGAGTCAG GGAGACGATG
    TTGCAGGCCC TGCGATTTGT CATTCAGGGA GCAGGAGCCA AGGTGGACCC CGCCATCAGG
    AAGAACATCA CCACCACACT GCTGAGCATG CTGGGACATG ATGAGGATGC CACAAGAATG
    GCCTCTGCGG GCTGTATTGG TGAACTCTGT GCCTTTCTAT CAGAAGAGGA GCTGAAGAGT
    GTCTTACAGC AGCACATCCT CGCGGATGTG TCTGGCGTGG ACTGGATGGT TCGTCATGGG
    CGTAGTTTGG CTCTGTCTAT CGCCGTGAAG TCTGCTCCTG CTCAGCTGTG TGTGGATGAG
    TACAACTCCT CTGTGCTGGA GGCCGTCTTG TCCAGCGCCA CCGCTGACAG AATCCCCATC
    GCCTGCAGTG GAATCAGAGC CATGGGTTTC CTGATGAGAC ACCAGCTCCG CACTGGAGGC
    TCAGAGAGTG TATCGCCCCG CATCATCACA CAGTTCATCA AGTGTCTCCA AAACCAGTCG
    AGTGACATTC GTCTGGTGGC AGAGCGGGTG TTGTGGTGGG TCTGGAAGGA GGACGAGACC
    CCCATTCTGG AGCCCAGTCT GGTCAAACCG CTCATCAAAG CACTGCTGGA CAACACCAAA
    GACAAGAACA CCAGCGTCCG AGCGCAGAGC GAACACACAC TCGTCAGCCT GCTGAGACTG
    CGGCGCGGAG AACACAACAT GCAGAGCATC AGTGCCATCC TGGACTCGGC CAGTAATGAC
    CTGCTGTCCG AATGTTACCG CAGATCCTTG AAGAAGATCT CCAGCCTTCC AGACTCCAAT
    GAAGAGATCG ATGATACCAT CCTCACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60004
    Clone ID Related Accession (Same CDS sequence) XM_009303726.3 , XM_009303726.2
    Accession Version XM_009303726.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product eIF-2-alpha kinase activator GCN1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) and transcript sequence (GDQH01001815.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 62 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGG ACAGGTTTCA GACACACTGA AGAGATTCGC TGTGAAAGTC 
    ACGTCATCCA GTGTTAAAGA GCGCAAAGAA ATTCTTGGAG AACTGAAGGA ATGTGTGAAG
    GGAAAGGAGC TTCCAGAGCC GGCCATCAAA GGACTGTGCA GACTCTTCTA TCTCACGCTG
    CACAGATACA GGGATGCTGC GTCTCGGCGA GCATTACTGT CCGTCATCGA GGTGCTGGTT
    CAGTCTCAGC CTGATGTGGT GGCCAGTAAT CTGCCTTCAG GTCTGCTCAG CTGCGGTGTG
    GTCAGTAGAG GAGCGATGCC CGGGAAGAGT TCGGCGTCTG GAGCGTGCTG CGCTCTGCCC
    TGGACCTGTC TGATCGTCCG CTTCGTCTTT CCTACCGCAG ACAAGAGAGA AGGTGCCAAC
    TGGAAGAAAC TGGTGGAGGT TCAGAGTTTG CTGCTGGCAG AGGCAGTAGG CGGAGCCTCA
    GGGAACGCTC TCAAGTCAAT CAACAAACAC TTCAACAAGC TGTGGAAGGA GCACCCGGGT
    CTGGTGGATC AGTACATGAG CACTGTGTTG AGTCTGGATC AGAGTTGTGT GTTTGTTCCT
    CTGCTGGGGT TGTGTGTTGA TTTCTGCACA GCACAGAAAG ACATCGCCAC TGTCAACAAA
    CACAAGGCAG CTCTCCTGGA TCTCTACATG AAGACGGTGC TGATGAGTAA AACCAGACCA
    CTTCAGCACA TACTGGAAAA GAGCGGCTCT ATTCTTCGCC ACATGTCTCA CGCTGAGTTT
    AAGGAGCAGC TGCTGCCCAC ATTACAGAAA GCTCTTCTGC GCAGCCCTGA GAACTGCATG
    CAGACAATCT CCTCCATGTT GGCGTCTCTC ACTCTGGATC TGAGTCAGTA CGCGCTGGAC
    ATCGGAAAGG GCATCTCCAG TCAGCTGAAG GCTAATAACC CAGAGCTGAT GGAGCAGGCG
    GTGGCGGCGC TGCAGAATCT GACCCAGCAG TGCAGTGACC CAAGCGCCGT CCAGGACCTC
    GCCAAACACC TCTTCAACAT CCTCGCAGGC TCAGAGGGGA AGCTGACTGT TGTGGCCCAG
    AAGATGAGCG TCCTCTCAGG AATCAGGAGC TGTAGTCATC ACGCTGTCTC TGGCTCTTCT
    TCTCAGTCTC TGAGCTCCAG CGTGGCTGTG ATGTTCATCC CATACCTGCA GCAGGAAGTC
    CACGAGGGGA CGCTGGTCCA TGCAGTGTCG GTGTTGTCTC AGTGGACGAG CCGCCTGACG
    CTGGATGTGC CCTCAGAGTT GAGAGACTGG TTTAAAAAAG CCTTCACCCT GAAGACGTCC
    ACATCCGCAG TGCGACACGC GTATCTGCAG GCCATGATCG GTGCTTTTAA AGGTGACTCT
    CTCAGCCAGG CTGTAGACTT CCTGCCTCTG CTCATTCAGA CGGTGGAGAA AGCTGCAGCT
    CAGAATACCC AGCATACACT GCTGTCTGAG GCCGTGGCTG CATCTGTGCT GCTCTGTCGG
    CTCTCTGTGC TGGACTCTGT GTCTGAAGCG AAGCTGACGT CCTTCTGGAA CCTGATCCTG
    GATGAGAAGA AGCCTTTGTT CACCACTGAG AAGTTCCTCT CTCAGGCCAG TGAAGAAGCC
    CTTTGCACCG TTCTACTGCT CTGTGAAAGA CTGTTCCTGG ATCAGCCACA AAGACTCAAC
    AACAGCAAGT CTCAGATGTA CCATCAGGCC ACAGTGATGC CGCTGCTGTC CCGTCTGTGG
    CCCGTACGCA AACGCGCTCA GCAAACAGTG AAGAAGCTCC TGTCCTCGCT GGGTGGATCC
    AGTCTGGCCC ACGGCCTTCT GGCAGAACTG CGTGTAGTTA TTAACAAACA CAAGATTCTG
    CCCGCAGACG TGCTGTATAC AGAGACGGGT GAGCTGACTG AGACGGGCCG CTCGTATGTG
    TCTCCGCGGG TCCTGCAGGA GGCTTTGAGT GTCATCTGCT CAGTTGCGGC CCAGTGGAAC
    GCCCCGGATG AGACAGAGAA ACTGGCCCTG GAGATTCTCA TCGTTACCCA TCATCCCTCT
    ATCGTGGCTG TCCGGTCTGA GCTGTGGTCT TCTCTGCTGT CTGCTATGAA GCTGGACGCT
    GCTCAATTCA TCGACAAACA CCTGGAAGTG ATTCTACCTC GCCTGCTGGA AGCCAACACA
    GACAATCAGG CTGTTCAGAA TGCTGTCGGT TCTCTGTCTC TGCTGTCTCC CAACAAACTG
    CTGCCGCGGG TCATGGATCA CGTGACCCAG CGATTGTCCA ATCCTGCACT GAGTCTGGTG
    ACGCGTCAGG AATACGCAAT CATGCAAACT CCAGAGGGAG AACTTTACGA CAAGAGCATC
    ATTATGAGTG CACAGCAGGA GAGCACAAAG AAAGGCAACA TGAAGAGAGA GAATAAAGCG
    TATTCCTTCA AAGAGCAGAT CATTGAACTG GAGCTTCAGG AGGAGATCAA GAAGAAGAAG
    GGCATCAAAG AGGAGATCCA GCTGACTAGC AAACAGAAGG AGATGATGCA GATCCAGCTG
    GAGAAAGAGT CTACTATCCG CAAGCGTCTG CAGGAGCTGG ACATGGAGCT GCTGTGTGCT
    GTGGGTTTGC TGGAAGCCGC TCTGGCTCGT CGTCCTCCGC TCATCTGGAT GCACCTTCCA
    GCACTGGTGC AGGTTCTCCT GCCGCTCCTG CAGTCTCCTC TGGCCGCTTC GTTGATAAAG
    CGTGTGTTTC TGCAGATCGG TGTCACTCTA ATGCCTAAAG AACTGCACCA GCTGGCGGTT
    CTGGTGGGTC ATGTGACCTT GCGTCTGCTG AAGCCAGAGT GTGATTTGGA TCCAGCCTGG
    GCCCAAGAAG ACCTGAACAC AGCGACACAG CGAACCATCT CACTGCTGCA CCAAAGAGAC
    GGCAAAACAG GATCAGATGT GCTGGTGTTG TCGGCTCCAG CGTTCTCCTT CTGTTTCCCG
    CTGCTGCAGG CTGTGTTGAG TCAGAGCAGC GGATCCTCAG AGGAGTCTGA GCTCATGATG
    ACGCGAGCGC TGCAGGTCAT CAACACACAC GCACAGCTGC GGGCGCAGGA CGACACCACC
    GACACCTTCA TAGATGAGAA TGGCCCAGAG CTGCTGCCGA GGGTCAGTAT GCTGCAGCTG
    CTGACCAAAG TGATCGGGAC GTCCACTCCT CGCCTTCAGG TTCTGGCGTC CAGCTGTCTG
    ACAGCTCTGT GTGGCAGTGC TGGAGGTCAG GAGGGCTGTG CTCTCGCTGA ACAACCTGAG
    ATCGATGTTC TGCTGGAGGC TTTACTGTCC TCCTGTTTCT CCGTCAGAGA CGCTGCGCTC
    CGGGGTCTGC TGGAGATGGA GCTGGCGTTA CCCACTGACA GCACTGATGT CAATGGCTTG
    AAGATGTTGA GGAGGCTCTG GGTGGCCAAG TTTGATGTGG AGGAGGAGGC CAGGGCTCTG
    GCGGAGAAAC TGTGGCAGGC TCTGTGTCTG GATCTGGTGC CGGAGCTGTG TCCACTGCTG
    ATTGAGGATG TGATCCAGCA TGAGGAGGCG GTGCGTTCAG CCGGTGCGGA GGCTTTGTCC
    AGCGCCGTCA GTCAGTATCA GGAGCAGTCA GCGACTGTCC TCTCACAGCT CACTCAACTC
    TACCATCAGA AGCTATATAG GCCGCCTCCA GTTCTGGATG CTCTTGGACG CGTTATTTCT
    GAAGCACCTC CTGATCAATG GGAGGCCAGG TGTGGCATTG CTCTTGCGCT GAACAAACTG
    GCTGAGTACC TGGACGAGAG TCAGGTGACG CCACTCTTCC TGTTCTTTGT GCCGGACGCT
    CTGAATGACA GACACCCTGA GGTGCGGCGC TGCATGCTGG ACGCGGCACT GTCTGCTCTT
    AACACACACG GCAAGGATAA CGTGAGCTCT CTGCTGCCCG TGTTTGAGGA GTTTCTGAAG
    AACGCACCGC AGGACGCCAG CTATGACTCT GTGCGGCAGA GTGTCGTCAT CCTCATGGGC
    TCGCTGGCCA AACATCTGGA CAAGAGCGAC CCCAAAGTCA AACCCATCGT GGCCAAGCTG
    ATCACAGCCC TTTCCACTCC CTCACAGCAG GTTCAGGAGT CGGTGGCCAG TTGCCTTCCT
    CCTCTGGTTC CTGCTATAAA GGAGGATGCA GGAGGGATGG TGCGGAAACT CCTGCAGCTG
    CTTCTGGAGA GCGACAAGTA CGCCGAACGC AAAGGAGCCG CATACGGACT GGCCGGACTG
    GTCAAAGGTC TGGGCATCTT ATCACTCAAA CAGCAGGAGA TCATGACCAC CCTGACGGAC
    GCCATTCAGG ACAAGAAGAA CTCCAGACGC AGAGAAGGGG CTCTGTTTGC GTTTGAGATG
    TTGTGTAACA TGCTGGGGAA GTTATTCGAG CCATATGTGG TTCATGTCCT CCCTCACTTG
    CTGCTGTGTT TCGGTGATGG GAATCAGTAC GTCAGAGAGG CTGCAGATGA CTGTGCTAAG
    GCTGTGATGA GGAATCTGAG CGCTCACGGG GTGAAGCTGG TTCTGCCGTC TCTGCTGGTG
    GCGCTGGAGG AGGAGTCCTG GAGAACCAAA GCAGGCTCAG TGGAGTTGTT GGGTGCGATG
    GCGTACTGCG CTCCTAAACA GCTGTCCTCA TGTCTGCCCA ACATTGTCCC GAAGCTGACA
    GAGGTTCTGA CAGACTCTCA TGTGAAGGTG CAGAAGGCCG GACAGCAGGC GCTTCGACAG
    ATCGGCTCCG TCATCCGCAA CCCTGAGATC CTCGCGATCA CCCCGATCCT GCTGGACGCT
    CTGACTGACC CGTCTCACAG GACGCAGCAC TGCCTGCAGA CACTGCTGGA CACCAAGTTT
    GTGCATTTCA TCGATGCTCC ATCTCTGGCT CTGATCATGC CCATCGTCCA GAGAGCCTTC
    CAGGACAGAT CCACCGACAC GCGCAAGATG GCGGCGCAGA TCATCGGAAA CATGTACTCA
    CTCACTGACC AGAAGGATCT GTCTCCGTAT TTGCCCAGTG TGATTCCAGG CCTCAAAGCA
    TCTCTGCTGG ACCCTGTGCC AGAGGTGCGG ACGGTGTCAG CGAAGGCTCT GGGTGCGATG
    GTGAAGGGAA TGGGTGAGTC CTCCTTTGAG GATCTGCTGC CGTGGCTGAT GGAGACTCTG
    GCGTCTGAAC AGAGCTCTGT GGATCGATCA GGAGCTGCGC AAGGTCTGGC TGAGGTGATG
    GCGGCTCTAG GTGTGGAGAA GCTGGATAAA CTGATGCCAG ATGTGGTTCA GACAGCCAGT
    AAAGTGGACA TCGCCTCTCA CGTCCGAGAC GGATATATCA TGATGTTTAT CTACCTTCCT
    CTGACCTTCG GGGACAAGTT CACCCCATAT GTGGGTCCCA TCATCCCCTG CATCCTAAAG
    GCTCTGGCTG ATGAGAACGA GTATGTTCGA GACACAGCTC TGCGTGCGGG TCAGAGGATC
    ATCAGCATGT ACGCAGAGAC GGCCATCGCT CTACTGCTGC CCGAGCTGGA GCAGGGCCTG
    TTTGATGACC TCTGGAGGAT CAGATTCAGC TCTGTGCAGT TGCTAGGAGA CCTGCTGTTC
    CATATCTCGG GGGTAACAGG AAAGATGACA ACAGAAACAG CATCAGAGGA TGACAACTTT
    GGCACGGCCC AGTCAAATAA GGCCATTATC TCAGCTCTGG GTGCAGAGAG GCGTAACCGT
    GTGCTGTCTG GACTCTACAT GGGCCGCTCA GACACACAGC TGGTGGTCCG ACAGGCGTCT
    CTTCATGTGT GGAAGATTGT GGTGTCAAAC ACTCCTCGAA CCCTCAGAGA AATCCTGCCC
    ACACTCTTCA CGCTGCTGCT GGGCTTCCTG GCCTCCACCT GCCCCGACAA GAGAACGATC
    GCCGCTCGAA CCCTGGGTGA CCTGGTGAGA AAGCTGGGAG AGAAGATCCT GCCTGAGATC
    ATTCCCATCC TGGAGGACGG TCTGCGTTCA GACAAGAGTG ACGAGCGCCA GGGTGTCTGC
    ATCGGCCTTA GCGAGATCAT GAAGTCCACC AGCAAAGACG CTGTTCTGGT GTTCTCCGAG
    TCTCTGGTGC CCACCGTGCG CAAGGCTCTG TGTGACCCGC TGGAGGAGGT TCGAGAAGCG
    GCTGCTAAAA CATTCGAGCA GCTTCACACC ACCATCGGTC ACCAGGCCCT GGACGACATC
    CTGCCCGCAC TGCTCAAACA ACTGGAGGAC GAGGAGACGT CAGAGTTTGC TCTGGATGGA
    CTGAAGCAGG TCATGGCTGT GAAGAGTCGC TCAGTCCTGC CGTACCTGGT TCCCAAGCTG
    ACGGCTCCTC CGGTGAACAC GCGTGTGCTG GCGTTTCTGT CTGCGGTGGC GGGAGATGCT
    CTGACGCGGC ACCTCGGCGT CATCCTGCCC GCTCTCCTCT CCTCTCTCAA AGACAAGCTG
    GGCACAGAGG AGAGTCTGCA GGAACTGAGC AACTGTCAGA CGGTGATTCT GTCAGTGGAG
    GATGAAGTGG GTCAGAGGAT CATCATTGAA GATCTGCTGG AGGCGACGCG AGGAGCGGAC
    GCTGGGCTCA GACAGGCCTC AATCACCATC CTCAACGGCT ATTTCTCCCG CACACGGCTG
    GACTACAGCG CTCACACACG CACACTGCTG TCAGGACTCA TGCGTCTCAT GAATGACCCC
    AACACTGAGG TGCTGAGCCA GAGCTGGGAC ACCATCAACT CCATTACAAA GAAACTGGAT
    GCGGGCAGTC AGCTCTCTCT GATTGATGAC CTGCACAGGG ACATCCGCTC TGCTGCTGCT
    GAGGTCAAAG GTCAACACTT ACCTGGCTTC TGCCTGCCCA AGAAGGGAGT GACCTGCATT
    CTCCCTGTGC TGAGGGAAGG TGTGCTGACA GGAAGTCCAG AGCAGAAAGA GGAAGCGGCT
    CGAGCTTTAG GTGGAGTCAT TAAACTGACC TCAGCGGAGG CTCTGCGGCC GTCAGTGGTC
    AACATCACTG GACCGCTCAT CCGAATCCTG GGAGACCGAT TCGCATGGAA CGTCAAAACT
    GCTCTTCTGG AGACCCTTAC ACTACTGCTG GCCAAAGTGG GCATCGCTCT GAAGCCCTTC
    CTGCCGCAGC TGCAGACCAC CTTCCTAAAG GCGTTGCAGG ACTCCAGTCG GGCTGTGAGG
    CTGCGGGCGG CGGAGGCTCT GGGGCAGCTC GTTTCCATCC ACACCAAAGT AGATCCACTG
    TTCACTGAGC AGCTGGCAGC CATACGCAAC GCTGAGGACT CTGGAGTCAG GGAGACGATG
    TTGCAGGCCC TGCGATTTGT CATTCAGGGA GCAGGAGCCA AGGTGGACCC CGCCATCAGG
    AAGAACATCA CCACCACACT GCTGAGCATG CTGGGACATG ATGAGGATGC CACAAGAATG
    GCCTCTGCGG GCTGTATTGG TGAACTCTGT GCCTTTCTAT CAGAAGAGGA GCTGAAGAGT
    GTCTTACAGC AGCACATCCT CGCGGATGTG TCTGGCGTGG ACTGGATGGT TCGTCATGGG
    CGTAGTTTGG CTCTGTCTAT CGCCGTGAAG TCTGCTCCTG CTCAGCTGTG TGTGGATGAG
    TACAACTCCT CTGTGCTGGA GGCCGTCTTG TCCAGCGCCA CCGCTGACAG AATCCCCATC
    GCCTGCAGTG GAATCAGAGC CATGGGTTTC CTGATGAGAC ACCAGCTCCG CACTGGAGGC
    TCAGAGAGTG TATCGCCCCG CATCATCACA CAGTTCATCA AGTGTCTCCA AAACCAGTCG
    AGTGACATTC GTCTGGTGGC AGAGCGGGTG TTGTGGTGGG TCTGGAAGGA GGACGAGACC
    CCCATTCTGG AGCCCAGTCT GGTCAAACCG CTCATCAAAG CACTGCTGGA CAACACCAAA
    GACAAGAACA CCAGCGTCCG AGCGCAGAGC GAACACACAC TCGTCAGCCT GCTGAGACTG
    CGGCGCGGAG AACACAACAT GCAGAGCATC AGTGCCATCC TGGACTCGGC CAGTAATGAC
    CTGCTGTCCG AATGTTACCG CAGATCCTTG AAGAAGATCT CCAGCCTTCC AGACTCCAAT
    GAAGAGATCG ATGATACCAT CCTCACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302001.2
    CDS45..8054
    Translation

    Target ORF information:

    RefSeq Version XM_009303726.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GCN1 eIF2 alpha kinase activator homolog (gcn1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303726.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGG ACAGGTTTCA GACACACTGA AGAGATTCGC TGTGAAAGTC 
    ACGTCATCCA GTGTTAAAGA GCGCAAAGAA ATTCTTGGAG AACTGAAGGA ATGTGTGAAG
    GGAAAGGAGC TTCCAGAGCC GGCCATCAAA GGACTGTGCA GACTCTTCTA TCTCACGCTG
    CACAGATACA GGGATGCTGC GTCTCGGCGA GCATTACTGT CCGTCATCGA GGTGCTGGTT
    CAGTCTCAGC CTGATGTGGT GGCCAGTAAT CTGCCTTCAG GTCTGCTCAG CTGCGGTGTG
    GTCAGTAGAG GAGCGATGCC CGGGAAGAGT TCGGCGTCTG GAGCGTGCTG CGCTCTGCCC
    TGGACCTGTC TGATCGTCCG CTTCGTCTTT CCTACCGCAG ACAAGAGAGA AGGTGCCAAC
    TGGAAGAAAC TGGTGGAGGT TCAGAGTTTG CTGCTGGCAG AGGCAGTAGG CGGAGCCTCA
    GGGAACGCTC TCAAGTCAAT CAACAAACAC TTCAACAAGC TGTGGAAGGA GCACCCGGGT
    CTGGTGGATC AGTACATGAG CACTGTGTTG AGTCTGGATC AGAGTTGTGT GTTTGTTCCT
    CTGCTGGGGT TGTGTGTTGA TTTCTGCACA GCACAGAAAG ACATCGCCAC TGTCAACAAA
    CACAAGGCAG CTCTCCTGGA TCTCTACATG AAGACGGTGC TGATGAGTAA AACCAGACCA
    CTTCAGCACA TACTGGAAAA GAGCGGCTCT ATTCTTCGCC ACATGTCTCA CGCTGAGTTT
    AAGGAGCAGC TGCTGCCCAC ATTACAGAAA GCTCTTCTGC GCAGCCCTGA GAACTGCATG
    CAGACAATCT CCTCCATGTT GGCGTCTCTC ACTCTGGATC TGAGTCAGTA CGCGCTGGAC
    ATCGGAAAGG GCATCTCCAG TCAGCTGAAG GCTAATAACC CAGAGCTGAT GGAGCAGGCG
    GTGGCGGCGC TGCAGAATCT GACCCAGCAG TGCAGTGACC CAAGCGCCGT CCAGGACCTC
    GCCAAACACC TCTTCAACAT CCTCGCAGGC TCAGAGGGGA AGCTGACTGT TGTGGCCCAG
    AAGATGAGCG TCCTCTCAGG AATCAGGAGC TGTAGTCATC ACGCTGTCTC TGGCTCTTCT
    TCTCAGTCTC TGAGCTCCAG CGTGGCTGTG ATGTTCATCC CATACCTGCA GCAGGAAGTC
    CACGAGGGGA CGCTGGTCCA TGCAGTGTCG GTGTTGTCTC AGTGGACGAG CCGCCTGACG
    CTGGATGTGC CCTCAGAGTT GAGAGACTGG TTTAAAAAAG CCTTCACCCT GAAGACGTCC
    ACATCCGCAG TGCGACACGC GTATCTGCAG GCCATGATCG GTGCTTTTAA AGGTGACTCT
    CTCAGCCAGG CTGTAGACTT CCTGCCTCTG CTCATTCAGA CGGTGGAGAA AGCTGCAGCT
    CAGAATACCC AGCATACACT GCTGTCTGAG GCCGTGGCTG CATCTGTGCT GCTCTGTCGG
    CTCTCTGTGC TGGACTCTGT GTCTGAAGCG AAGCTGACGT CCTTCTGGAA CCTGATCCTG
    GATGAGAAGA AGCCTTTGTT CACCACTGAG AAGTTCCTCT CTCAGGCCAG TGAAGAAGCC
    CTTTGCACCG TTCTACTGCT CTGTGAAAGA CTGTTCCTGG ATCAGCCACA AAGACTCAAC
    AACAGCAAGT CTCAGATGTA CCATCAGGCC ACAGTGATGC CGCTGCTGTC CCGTCTGTGG
    CCCGTACGCA AACGCGCTCA GCAAACAGTG AAGAAGCTCC TGTCCTCGCT GGGTGGATCC
    AGTCTGGCCC ACGGCCTTCT GGCAGAACTG CGTGTAGTTA TTAACAAACA CAAGATTCTG
    CCCGCAGACG TGCTGTATAC AGAGACGGGT GAGCTGACTG AGACGGGCCG CTCGTATGTG
    TCTCCGCGGG TCCTGCAGGA GGCTTTGAGT GTCATCTGCT CAGTTGCGGC CCAGTGGAAC
    GCCCCGGATG AGACAGAGAA ACTGGCCCTG GAGATTCTCA TCGTTACCCA TCATCCCTCT
    ATCGTGGCTG TCCGGTCTGA GCTGTGGTCT TCTCTGCTGT CTGCTATGAA GCTGGACGCT
    GCTCAATTCA TCGACAAACA CCTGGAAGTG ATTCTACCTC GCCTGCTGGA AGCCAACACA
    GACAATCAGG CTGTTCAGAA TGCTGTCGGT TCTCTGTCTC TGCTGTCTCC CAACAAACTG
    CTGCCGCGGG TCATGGATCA CGTGACCCAG CGATTGTCCA ATCCTGCACT GAGTCTGGTG
    ACGCGTCAGG AATACGCAAT CATGCAAACT CCAGAGGGAG AACTTTACGA CAAGAGCATC
    ATTATGAGTG CACAGCAGGA GAGCACAAAG AAAGGCAACA TGAAGAGAGA GAATAAAGCG
    TATTCCTTCA AAGAGCAGAT CATTGAACTG GAGCTTCAGG AGGAGATCAA GAAGAAGAAG
    GGCATCAAAG AGGAGATCCA GCTGACTAGC AAACAGAAGG AGATGATGCA GATCCAGCTG
    GAGAAAGAGT CTACTATCCG CAAGCGTCTG CAGGAGCTGG ACATGGAGCT GCTGTGTGCT
    GTGGGTTTGC TGGAAGCCGC TCTGGCTCGT CGTCCTCCGC TCATCTGGAT GCACCTTCCA
    GCACTGGTGC AGGTTCTCCT GCCGCTCCTG CAGTCTCCTC TGGCCGCTTC GTTGATAAAG
    CGTGTGTTTC TGCAGATCGG TGTCACTCTA ATGCCTAAAG AACTGCACCA GCTGGCGGTT
    CTGGTGGGTC ATGTGACCTT GCGTCTGCTG AAGCCAGAGT GTGATTTGGA TCCAGCCTGG
    GCCCAAGAAG ACCTGAACAC AGCGACACAG CGAACCATCT CACTGCTGCA CCAAAGAGAC
    GGCAAAACAG GATCAGATGT GCTGGTGTTG TCGGCTCCAG CGTTCTCCTT CTGTTTCCCG
    CTGCTGCAGG CTGTGTTGAG TCAGAGCAGC GGATCCTCAG AGGAGTCTGA GCTCATGATG
    ACGCGAGCGC TGCAGGTCAT CAACACACAC GCACAGCTGC GGGCGCAGGA CGACACCACC
    GACACCTTCA TAGATGAGAA TGGCCCAGAG CTGCTGCCGA GGGTCAGTAT GCTGCAGCTG
    CTGACCAAAG TGATCGGGAC GTCCACTCCT CGCCTTCAGG TTCTGGCGTC CAGCTGTCTG
    ACAGCTCTGT GTGGCAGTGC TGGAGGTCAG GAGGGCTGTG CTCTCGCTGA ACAACCTGAG
    ATCGATGTTC TGCTGGAGGC TTTACTGTCC TCCTGTTTCT CCGTCAGAGA CGCTGCGCTC
    CGGGGTCTGC TGGAGATGGA GCTGGCGTTA CCCACTGACA GCACTGATGT CAATGGCTTG
    AAGATGTTGA GGAGGCTCTG GGTGGCCAAG TTTGATGTGG AGGAGGAGGC CAGGGCTCTG
    GCGGAGAAAC TGTGGCAGGC TCTGTGTCTG GATCTGGTGC CGGAGCTGTG TCCACTGCTG
    ATTGAGGATG TGATCCAGCA TGAGGAGGCG GTGCGTTCAG CCGGTGCGGA GGCTTTGTCC
    AGCGCCGTCA GTCAGTATCA GGAGCAGTCA GCGACTGTCC TCTCACAGCT CACTCAACTC
    TACCATCAGA AGCTATATAG GCCGCCTCCA GTTCTGGATG CTCTTGGACG CGTTATTTCT
    GAAGCACCTC CTGATCAATG GGAGGCCAGG TGTGGCATTG CTCTTGCGCT GAACAAACTG
    GCTGAGTACC TGGACGAGAG TCAGGTGACG CCACTCTTCC TGTTCTTTGT GCCGGACGCT
    CTGAATGACA GACACCCTGA GGTGCGGCGC TGCATGCTGG ACGCGGCACT GTCTGCTCTT
    AACACACACG GCAAGGATAA CGTGAGCTCT CTGCTGCCCG TGTTTGAGGA GTTTCTGAAG
    AACGCACCGC AGGACGCCAG CTATGACTCT GTGCGGCAGA GTGTCGTCAT CCTCATGGGC
    TCGCTGGCCA AACATCTGGA CAAGAGCGAC CCCAAAGTCA AACCCATCGT GGCCAAGCTG
    ATCACAGCCC TTTCCACTCC CTCACAGCAG GTTCAGGAGT CGGTGGCCAG TTGCCTTCCT
    CCTCTGGTTC CTGCTATAAA GGAGGATGCA GGAGGGATGG TGCGGAAACT CCTGCAGCTG
    CTTCTGGAGA GCGACAAGTA CGCCGAACGC AAAGGAGCCG CATACGGACT GGCCGGACTG
    GTCAAAGGTC TGGGCATCTT ATCACTCAAA CAGCAGGAGA TCATGACCAC CCTGACGGAC
    GCCATTCAGG ACAAGAAGAA CTCCAGACGC AGAGAAGGGG CTCTGTTTGC GTTTGAGATG
    TTGTGTAACA TGCTGGGGAA GTTATTCGAG CCATATGTGG TTCATGTCCT CCCTCACTTG
    CTGCTGTGTT TCGGTGATGG GAATCAGTAC GTCAGAGAGG CTGCAGATGA CTGTGCTAAG
    GCTGTGATGA GGAATCTGAG CGCTCACGGG GTGAAGCTGG TTCTGCCGTC TCTGCTGGTG
    GCGCTGGAGG AGGAGTCCTG GAGAACCAAA GCAGGCTCAG TGGAGTTGTT GGGTGCGATG
    GCGTACTGCG CTCCTAAACA GCTGTCCTCA TGTCTGCCCA ACATTGTCCC GAAGCTGACA
    GAGGTTCTGA CAGACTCTCA TGTGAAGGTG CAGAAGGCCG GACAGCAGGC GCTTCGACAG
    ATCGGCTCCG TCATCCGCAA CCCTGAGATC CTCGCGATCA CCCCGATCCT GCTGGACGCT
    CTGACTGACC CGTCTCACAG GACGCAGCAC TGCCTGCAGA CACTGCTGGA CACCAAGTTT
    GTGCATTTCA TCGATGCTCC ATCTCTGGCT CTGATCATGC CCATCGTCCA GAGAGCCTTC
    CAGGACAGAT CCACCGACAC GCGCAAGATG GCGGCGCAGA TCATCGGAAA CATGTACTCA
    CTCACTGACC AGAAGGATCT GTCTCCGTAT TTGCCCAGTG TGATTCCAGG CCTCAAAGCA
    TCTCTGCTGG ACCCTGTGCC AGAGGTGCGG ACGGTGTCAG CGAAGGCTCT GGGTGCGATG
    GTGAAGGGAA TGGGTGAGTC CTCCTTTGAG GATCTGCTGC CGTGGCTGAT GGAGACTCTG
    GCGTCTGAAC AGAGCTCTGT GGATCGATCA GGAGCTGCGC AAGGTCTGGC TGAGGTGATG
    GCGGCTCTAG GTGTGGAGAA GCTGGATAAA CTGATGCCAG ATGTGGTTCA GACAGCCAGT
    AAAGTGGACA TCGCCTCTCA CGTCCGAGAC GGATATATCA TGATGTTTAT CTACCTTCCT
    CTGACCTTCG GGGACAAGTT CACCCCATAT GTGGGTCCCA TCATCCCCTG CATCCTAAAG
    GCTCTGGCTG ATGAGAACGA GTATGTTCGA GACACAGCTC TGCGTGCGGG TCAGAGGATC
    ATCAGCATGT ACGCAGAGAC GGCCATCGCT CTACTGCTGC CCGAGCTGGA GCAGGGCCTG
    TTTGATGACC TCTGGAGGAT CAGATTCAGC TCTGTGCAGT TGCTAGGAGA CCTGCTGTTC
    CATATCTCGG GGGTAACAGG AAAGATGACA ACAGAAACAG CATCAGAGGA TGACAACTTT
    GGCACGGCCC AGTCAAATAA GGCCATTATC TCAGCTCTGG GTGCAGAGAG GCGTAACCGT
    GTGCTGTCTG GACTCTACAT GGGCCGCTCA GACACACAGC TGGTGGTCCG ACAGGCGTCT
    CTTCATGTGT GGAAGATTGT GGTGTCAAAC ACTCCTCGAA CCCTCAGAGA AATCCTGCCC
    ACACTCTTCA CGCTGCTGCT GGGCTTCCTG GCCTCCACCT GCCCCGACAA GAGAACGATC
    GCCGCTCGAA CCCTGGGTGA CCTGGTGAGA AAGCTGGGAG AGAAGATCCT GCCTGAGATC
    ATTCCCATCC TGGAGGACGG TCTGCGTTCA GACAAGAGTG ACGAGCGCCA GGGTGTCTGC
    ATCGGCCTTA GCGAGATCAT GAAGTCCACC AGCAAAGACG CTGTTCTGGT GTTCTCCGAG
    TCTCTGGTGC CCACCGTGCG CAAGGCTCTG TGTGACCCGC TGGAGGAGGT TCGAGAAGCG
    GCTGCTAAAA CATTCGAGCA GCTTCACACC ACCATCGGTC ACCAGGCCCT GGACGACATC
    CTGCCCGCAC TGCTCAAACA ACTGGAGGAC GAGGAGACGT CAGAGTTTGC TCTGGATGGA
    CTGAAGCAGG TCATGGCTGT GAAGAGTCGC TCAGTCCTGC CGTACCTGGT TCCCAAGCTG
    ACGGCTCCTC CGGTGAACAC GCGTGTGCTG GCGTTTCTGT CTGCGGTGGC GGGAGATGCT
    CTGACGCGGC ACCTCGGCGT CATCCTGCCC GCTCTCCTCT CCTCTCTCAA AGACAAGCTG
    GGCACAGAGG AGAGTCTGCA GGAACTGAGC AACTGTCAGA CGGTGATTCT GTCAGTGGAG
    GATGAAGTGG GTCAGAGGAT CATCATTGAA GATCTGCTGG AGGCGACGCG AGGAGCGGAC
    GCTGGGCTCA GACAGGCCTC AATCACCATC CTCAACGGCT ATTTCTCCCG CACACGGCTG
    GACTACAGCG CTCACACACG CACACTGCTG TCAGGACTCA TGCGTCTCAT GAATGACCCC
    AACACTGAGG TGCTGAGCCA GAGCTGGGAC ACCATCAACT CCATTACAAA GAAACTGGAT
    GCGGGCAGTC AGCTCTCTCT GATTGATGAC CTGCACAGGG ACATCCGCTC TGCTGCTGCT
    GAGGTCAAAG GTCAACACTT ACCTGGCTTC TGCCTGCCCA AGAAGGGAGT GACCTGCATT
    CTCCCTGTGC TGAGGGAAGG TGTGCTGACA GGAAGTCCAG AGCAGAAAGA GGAAGCGGCT
    CGAGCTTTAG GTGGAGTCAT TAAACTGACC TCAGCGGAGG CTCTGCGGCC GTCAGTGGTC
    AACATCACTG GACCGCTCAT CCGAATCCTG GGAGACCGAT TCGCATGGAA CGTCAAAACT
    GCTCTTCTGG AGACCCTTAC ACTACTGCTG GCCAAAGTGG GCATCGCTCT GAAGCCCTTC
    CTGCCGCAGC TGCAGACCAC CTTCCTAAAG GCGTTGCAGG ACTCCAGTCG GGCTGTGAGG
    CTGCGGGCGG CGGAGGCTCT GGGGCAGCTC GTTTCCATCC ACACCAAAGT AGATCCACTG
    TTCACTGAGC AGCTGGCAGC CATACGCAAC GCTGAGGACT CTGGAGTCAG GGAGACGATG
    TTGCAGGCCC TGCGATTTGT CATTCAGGGA GCAGGAGCCA AGGTGGACCC CGCCATCAGG
    AAGAACATCA CCACCACACT GCTGAGCATG CTGGGACATG ATGAGGATGC CACAAGAATG
    GCCTCTGCGG GCTGTATTGG TGAACTCTGT GCCTTTCTAT CAGAAGAGGA GCTGAAGAGT
    GTCTTACAGC AGCACATCCT CGCGGATGTG TCTGGCGTGG ACTGGATGGT TCGTCATGGG
    CGTAGTTTGG CTCTGTCTAT CGCCGTGAAG TCTGCTCCTG CTCAGCTGTG TGTGGATGAG
    TACAACTCCT CTGTGCTGGA GGCCGTCTTG TCCAGCGCCA CCGCTGACAG AATCCCCATC
    GCCTGCAGTG GAATCAGAGC CATGGGTTTC CTGATGAGAC ACCAGCTCCG CACTGGAGGC
    TCAGAGAGTG TATCGCCCCG CATCATCACA CAGTTCATCA AGTGTCTCCA AAACCAGTCG
    AGTGACATTC GTCTGGTGGC AGAGCGGGTG TTGTGGTGGG TCTGGAAGGA GGACGAGACC
    CCCATTCTGG AGCCCAGTCT GGTCAAACCG CTCATCAAAG CACTGCTGGA CAACACCAAA
    GACAAGAACA CCAGCGTCCG AGCGCAGAGC GAACACACAC TCGTCAGCCT GCTGAGACTG
    CGGCGCGGAG AACACAACAT GCAGAGCATC AGTGCCATCC TGGACTCGGC CAGTAATGAC
    CTGCTGTCCG AATGTTACCG CAGATCCTTG AAGAAGATCT CCAGCCTTCC AGACTCCAAT
    GAAGAGATCG ATGATACCAT CCTCACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.