lats2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lats2
Entrez Gene ID	567674
Full Name	large tumor suppressor kinase 2
Synonyms	si:ch211-173o4.2
General protein information	Preferred Names large tumor suppressor kinase 2 Names serine/threonine-protein kinase LATS2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167579.3	XP_005167636.1	serine/threonine-protein kinase LATS2 isoform X1
NM_001128256.1	NP_001121728.1	serine/threonine-protein kinase LATS2 isoform 2
NM_001123307.1	NP_001116779.1	serine/threonine-protein kinase LATS2 isoform 1
XM_005167579.4	XP_005167636.1	serine/threonine-protein kinase LATS2 isoform X1
XM_021478741.1	XP_021334416.1	serine/threonine-protein kinase LATS2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Lats2	NP_056586.2
Rattus norvegicus (Norway rat)	Lats2	NP_001100737.1
Danio rerio (zebrafish)	lats2	NP_001121728.1
Pan troglodytes (chimpanzee)	LATS2	XP_001149147.1
Gallus gallus (chicken)	LATS2	XP_417143.3
Xenopus tropicalis (tropical clawed frog)	lats2	NP_001096174.1
Homo sapiens (human)	LATS2	NP_055387.2
Canis lupus familiaris (dog)	LATS2	XP_534537.3

Gene Ontology
Bibliography

Related articles in PubMed

A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth.
Mayrhofer M, Gourain V, Reischl M, Affaticati P, Jenett A, Joly JS, Benelli M, Demichelis F, Poliani PL, Sieger D, Mione M
Disease models & mechanisms10(1)15-28(2017 01)

Yap and Taz regulate retinal pigment epithelial cell fate.
Miesfeld JB, Gestri G, Clark BS, Flinn MA, Poole RJ, Bader JR, Besharse JC, Wilson SW, Link BA
Development (Cambridge, England)142(17)3021-32(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus AMOT is a direct substrate of Lats1/2 mediating functions of the Hippo pathway in endothelial cell migration and angiogenesis.
Title: Phosphorylation of angiomotin by Lats1/2 kinases inhibits F-actin binding, cell migration, and angiogenesis.

lats genes show distinctly different expression profiles during gastrulation. lats1 is almost ubiquitously expressed through development, and lats2 is more prominently expressed in the non-neural ectoderm region of zebrafish gastrula.
Title: Comparative expression of zebrafish lats1 and lats2 and their implication in gastrulation movements.

The following lats2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lats2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02824	NM_001128256.1 Latest version!	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa03045	NM_001123307.1 Latest version!	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15095	XM_005167579.4 Latest version!	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15095	XM_021478741.1 Latest version!	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa15095	XM_005167579.3	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lats2 ( NM_001128256.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121728.1 Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa02824
Clone ID Related Accession (Same CDS sequence)	NM_001128256.1
Accession Version	NM_001128256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase LATS2 isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF524199.1. On Jul 31, 2008 this sequence version replaced XM_001923461.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF524199.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGGCCGA AGACGTTCCC TGCCACTCTA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCACT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAATTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAAAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT TCTCAGCCCT CAGGAGGGCC TGGAGCACAT 
TATGACTACA GGTCGCATAT GATGGAGAAT TCCAGCTACG GGGTCAAAAG AAGTGCTTCC 
TTTCAGAATA AGATGCCCCA ACTAACTACA GAAAATTATG TCAATATGCC AAGCAAAGCA 
GCAATGGGCC AAAATGCTGG TGGCTATGCT CCCAACTTAT ATCTGCCACC ACATTCACAT 
CCACGCCAAG CTAGCCCCAC CTCACACCAG GTTCACATGA TGCAGCGCCC CCCAGGTGTT 
GCCCCTGGAG CTATGGGTGG GGAGTTCTCT GATGTTCCTC AAGGCCTTCT CACCCCTTCC 
AGGGCCAGTT TAAATCTTGA CCTGTATGAG CATCACTGGC CAGGAGCTCA AGGTCCTGAG 
GGGACTCCTC CAGCCAGGCA GCCACAAGGG CCCTTCAGAG GGGAGGTACG CGTCCCCAGT 
CGGACCAATT CTTTCAACAA CCACCAAAAG GTGACTGTGA GGCAGACATT GCCCCCAACA 
GCTAGTGTTG CTGGGAAGCC AGAGTCATCT TTGGGCCCAC CTAACACAAT AACTGCAGTG 
ACCTCTCCAC CAATTCAGCC ACCTGTTAAG AGTATACGGG TCATGAGGCC TGAGCCGAAG 
ACAGCAGTGG GACCATGCCA TCCAGGGTGG CTGAGTGCAC AATCCCCGGA AGCACAGGAT 
TCTCTTGGAT ACATCCCAGA TGAGAATTTT ACCCTGGAAC CCAGTCAAGA ACATCGATGC 
CCACCTCCAC CTTACCCCAA AACACTGCTT GTGTCTGGAT CTGGACCTGA GCCTGCATCC 
TTAGAGCCTG TAGCCATGAG TGGACTTCAA GATGTCAGTG CCACAGGTAG GACTATCCAG 
AGTTCCTCGG TAGGAAAGCA GGAGGAACCC ATCTTCAAAG ACAAAGTGAA AATGGGCAAA 
GGAGAAAAGG CTGTGAAGGA TAAGAAGCAG ATCCAGACAT CACCTGTGCC TGTACGAAAA 
AATGCCCGTG ATGAAGAGAA GAGGGAGTCT CGTATTAAGA GTTATTCTCC ATTTGCCTTT 
AAGTTCTACA TGGAGCAACA TGTGGAGAAT GTGATGAAGA CATACCAGCA GAAACTTAAT 
CGCAGAATGC AGCTGGAGCA AGAGATGTCC AAGGCTGGCC TTTCAGAGGC TGAGCAGGAA 
CAGATGAGAA AGATGCTTTA CCAGAAGGAA TCCAATTACA ATCGACTACG ACGTGCCAAG 
ATGGACAAAT CAATGTTTGT CAAAATCAAG ACACTCGGCA TTGGGGCCTT TGGTGAGGTG 
TGTCTAACCC GAAAAGTAGA CACAGGAGCC TTGTATGCAA TGAAGACTTT GCGAAAGAAA 
GACGTTCTTA ACCGAAATCA AGTAGCCCAT GTTAAAGCTG AACGGGACAT TTTAGCAGAG 
GCTGACAATG AGTGGGTAGT CAGGTTGTAC TATTCATTTC AAGACCGAGA CAATCTGTAT 
TTTGTCATGG ATTACATTCC TGGAGGAGAT ATGATGAGCT TGCTGATCCG TATGGGAGTT 
TTTCCAGAGG TCCTAGCCAG GTTTTATGTG GCAGAACTGA CACTGGCAAT TGAGAGTGTG 
CATAAGATGG GTTTCATTCA CAGAGATATC AAGCCAGACA ATATCCTCAT TGACCTGGAT 
GGACACATCA AACTGACTGA TTTTGGTCTA TGTACAGGGT TCCGATGGAC TCACAACTCA 
AAGTATTACC AAAAAGGGAA CCATATCCGA CAGGATAGCA TGGAGCCAAG TGACTCTTGG 
GATGATGTGT CTAATTGCCG TTGTGGTGAC CGGCTGATGA CTTTGGAACA GAGAGCAACT 
CGACAACACC AGCGCTGCCT GGCGCATTCT TTGGTGGGCA CCCCTAACTA CATTGCCCCT 
GAGGTGCTTT TGCGCAAAGG ATACACACAG CTTTGCGACT GGTGGAGTGT GGGAGTGATC 
CTGTTTGAGA TGCTGGTTGG CCAACCACCC TTTCTTGCTC CAACCCCAAC AGAGGCACAG 
CTTAAGGTGA TAAACTGGGA GAACACTTTG CAAGTGCCAC CACAAGTAAA ACTAAGCCCA 
GAGGCAGTCG ATATTATTGG CCAGCTTTGC TGCTCTGCTG AAGAGCGTCT AGGAGGCAAT 
GGGGCTGGAG AAATAAAGGC TCATCCTTTT TTTTCCGAGG TGGATTTCTC CAGCAATCTT 
CGGACACAAC CTGCACCATA TCGACCAAAA ATTGCCCACC CCATGGATAC ATCCAACTTT 
GATCCTGTTG AAGAGGAGGG AGGCCCAGGC GCCTGGAGTG ATAGTGGGGA TAGCACCCGC 
ACTTGGGACA CCCTCTGTTC TCCTCACGGC AAACACCCTG AACATGCCTT CTATGAGTTT 
ACTTTCCGAA GATTTTTTGA TGATAATGGT TGTCCATTCC GTTATCCCAA ACCCCTAGAG 
GCGACACAAA GCCTGTCCAG CAGTGACGGG GCCGGCAGCC TGAAGCCTGA GCTGGAGGAG 
GAAGAGGCCC ATGAAGAGGA AGAGCAAGGG GAAGGGTGTG AGCCGGTTTA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121728.1
CDS	248..3484
Translation	MRPKTFPATLYVGNTRQRLQDIKEGLKQPAKLVSQALHGGSSRGEGGRGGDSKGKDQAARQQQLRPPQKFNNYQSALREIRKSLRPYANDSGPGSSSGHPGEVSRQMLQDLVNAGCDQEMAVRALKQTGSRNIEAALEYISKMSYLDPHTEQIVRVIKQTSPGKSGMPNLMDHRTAMEGSGDGALPPYHQMGAPLYEGASYGAEGEMARAYMGGPPVMNYMPPPANPSQGSQPSGGPGAHYDYRSHMMENSSYGVKRSASFQNKMPQLTTENYVNMPSKAAMGQNAGGYAPNLYLPPHSHPRQASPTSHQVHMMQRPPGVAPGAMGGEFSDVPQGLLTPSRASLNLDLYEHHWPGAQGPEGTPPARQPQGPFRGEVRVPSRTNSFNNHQKVTVRQTLPPTASVAGKPESSLGPPNTITAVTSPPIQPPVKSIRVMRPEPKTAVGPCHPGWLSAQSPEAQDSLGYIPDENFTLEPSQEHRCPPPPYPKTLLVSGSGPEPASLEPVAMSGLQDVSATGRTIQSSSVGKQEEPIFKDKVKMGKGEKAVKDKKQIQTSPVPVRKNARDEEKRESRIKSYSPFAFKFYMEQHVENVMKTYQQKLNRRMQLEQEMSKAGLSEAEQEQMRKMLYQKESNYNRLRRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTGALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRDNLYFVMDYIPGGDMMSLLIRMGVFPEVLARFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGNHIRQDSMEPSDSWDDVSNCRCGDRLMTLEQRATRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTEAQLKVINWENTLQVPPQVKLSPEAVDIIGQLCCSAEERLGGNGAGEIKAHPFFSEVDFSSNLRTQPAPYRPKIAHPMDTSNFDPVEEEGGPGAWSDSGDSTRTWDTLCSPHGKHPEHAFYEFTFRRFFDDNGCPFRYPKPLEATQSLSSSDGAGSLKPELEEEEAHEEEEQGEGCEPVYV

Target ORF information:

RefSeq Version	NM_001128256.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128256.1

ORF Insert Sequence:

ATGAGGCCGA AGACGTTCCC TGCCACTCTA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCACT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAATTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAAAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT TCTCAGCCCT CAGGAGGGCC TGGAGCACAT 
TATGACTACA GGTCGCATAT GATGGAGAAT TCCAGCTACG GGGTCAAAAG AAGTGCTTCC 
TTTCAGAATA AGATGCCCCA ACTAACTACA GAAAATTATG TCAATATGCC AAGCAAAGCA 
GCAATGGGCC AAAATGCTGG TGGCTATGCT CCCAACTTAT ATCTGCCACC ACATTCACAT 
CCACGCCAAG CTAGCCCCAC CTCACACCAG GTTCACATGA TGCAGCGCCC CCCAGGTGTT 
GCCCCTGGAG CTATGGGTGG GGAGTTCTCT GATGTTCCTC AAGGCCTTCT CACCCCTTCC 
AGGGCCAGTT TAAATCTTGA CCTGTATGAG CATCACTGGC CAGGAGCTCA AGGTCCTGAG 
GGGACTCCTC CAGCCAGGCA GCCACAAGGG CCCTTCAGAG GGGAGGTACG CGTCCCCAGT 
CGGACCAATT CTTTCAACAA CCACCAAAAG GTGACTGTGA GGCAGACATT GCCCCCAACA 
GCTAGTGTTG CTGGGAAGCC AGAGTCATCT TTGGGCCCAC CTAACACAAT AACTGCAGTG 
ACCTCTCCAC CAATTCAGCC ACCTGTTAAG AGTATACGGG TCATGAGGCC TGAGCCGAAG 
ACAGCAGTGG GACCATGCCA TCCAGGGTGG CTGAGTGCAC AATCCCCGGA AGCACAGGAT 
TCTCTTGGAT ACATCCCAGA TGAGAATTTT ACCCTGGAAC CCAGTCAAGA ACATCGATGC 
CCACCTCCAC CTTACCCCAA AACACTGCTT GTGTCTGGAT CTGGACCTGA GCCTGCATCC 
TTAGAGCCTG TAGCCATGAG TGGACTTCAA GATGTCAGTG CCACAGGTAG GACTATCCAG 
AGTTCCTCGG TAGGAAAGCA GGAGGAACCC ATCTTCAAAG ACAAAGTGAA AATGGGCAAA 
GGAGAAAAGG CTGTGAAGGA TAAGAAGCAG ATCCAGACAT CACCTGTGCC TGTACGAAAA 
AATGCCCGTG ATGAAGAGAA GAGGGAGTCT CGTATTAAGA GTTATTCTCC ATTTGCCTTT 
AAGTTCTACA TGGAGCAACA TGTGGAGAAT GTGATGAAGA CATACCAGCA GAAACTTAAT 
CGCAGAATGC AGCTGGAGCA AGAGATGTCC AAGGCTGGCC TTTCAGAGGC TGAGCAGGAA 
CAGATGAGAA AGATGCTTTA CCAGAAGGAA TCCAATTACA ATCGACTACG ACGTGCCAAG 
ATGGACAAAT CAATGTTTGT CAAAATCAAG ACACTCGGCA TTGGGGCCTT TGGTGAGGTG 
TGTCTAACCC GAAAAGTAGA CACAGGAGCC TTGTATGCAA TGAAGACTTT GCGAAAGAAA 
GACGTTCTTA ACCGAAATCA AGTAGCCCAT GTTAAAGCTG AACGGGACAT TTTAGCAGAG 
GCTGACAATG AGTGGGTAGT CAGGTTGTAC TATTCATTTC AAGACCGAGA CAATCTGTAT 
TTTGTCATGG ATTACATTCC TGGAGGAGAT ATGATGAGCT TGCTGATCCG TATGGGAGTT 
TTTCCAGAGG TCCTAGCCAG GTTTTATGTG GCAGAACTGA CACTGGCAAT TGAGAGTGTG 
CATAAGATGG GTTTCATTCA CAGAGATATC AAGCCAGACA ATATCCTCAT TGACCTGGAT 
GGACACATCA AACTGACTGA TTTTGGTCTA TGTACAGGGT TCCGATGGAC TCACAACTCA 
AAGTATTACC AAAAAGGGAA CCATATCCGA CAGGATAGCA TGGAGCCAAG TGACTCTTGG 
GATGATGTGT CTAATTGCCG TTGTGGTGAC CGGCTGATGA CTTTGGAACA GAGAGCAACT 
CGACAACACC AGCGCTGCCT GGCGCATTCT TTGGTGGGCA CCCCTAACTA CATTGCCCCT 
GAGGTGCTTT TGCGCAAAGG ATACACACAG CTTTGCGACT GGTGGAGTGT GGGAGTGATC 
CTGTTTGAGA TGCTGGTTGG CCAACCACCC TTTCTTGCTC CAACCCCAAC AGAGGCACAG 
CTTAAGGTGA TAAACTGGGA GAACACTTTG CAAGTGCCAC CACAAGTAAA ACTAAGCCCA 
GAGGCAGTCG ATATTATTGG CCAGCTTTGC TGCTCTGCTG AAGAGCGTCT AGGAGGCAAT 
GGGGCTGGAG AAATAAAGGC TCATCCTTTT TTTTCCGAGG TGGATTTCTC CAGCAATCTT 
CGGACACAAC CTGCACCATA TCGACCAAAA ATTGCCCACC CCATGGATAC ATCCAACTTT 
GATCCTGTTG AAGAGGAGGG AGGCCCAGGC GCCTGGAGTG ATAGTGGGGA TAGCACCCGC 
ACTTGGGACA CCCTCTGTTC TCCTCACGGC AAACACCCTG AACATGCCTT CTATGAGTTT 
ACTTTCCGAA GATTTTTTGA TGATAATGGT TGTCCATTCC GTTATCCCAA ACCCCTAGAG 
GCGACACAAA GCCTGTCCAG CAGTGACGGG GCCGGCAGCC TGAAGCCTGA GCTGGAGGAG 
GAAGAGGCCC ATGAAGAGGA AGAGCAAGGG GAAGGGTGTG AGCCGGTTTA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats2 ( NM_001123307.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116779.1 Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa03045
Clone ID Related Accession (Same CDS sequence)	NM_001123307.1
Accession Version	NM_001123307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase LATS2 isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF524200.1. On Apr 11, 2008 this sequence version replaced XR_029051.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF524200.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCCGA AGACGTTCCC TGCCACTCCA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCGCT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAGTTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAGAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT GTTACCATGG GAAACCCGAT GGGACGCCCT 
GCGAGTATGG GTGCTTACTC CACAAACATG GCTCCTCAAA ATAATACTGG GAATCCAATG 
TACACCCCTG GTCCAGCCCA GCAAAAAGGA TACCCTAGTG GGATTGACCA GGCCATGATG 
GGGTACAGTG TGTCTGGACC ACCCATGCAA ATCCAGTCTC AGCCCTCAGG AGGGCCTGGA 
GCACATTATG ACTACAGGTC GCATATGATG GAGAATTCCA GCTACGGGGT CAAAAGAAGT 
GCTTCCTTTC AGAATAAGAT GCCCCAACTA ACTACAGAAA ATTATGTCAA TATGCCAGGC 
AAAGCAGCAA TGGGCCAAAA TGCTGGTGGC TATGCTCCCA ACTTATATCT GCCACCACAT 
TCACATCCAC GCCAAGCTAG CCCCACCTCA CACCAGGTTC ACATGATGCA GCGCCCCCCA 
GGTGTTGCCC CTGGAGCTAT GGGTGGGGAG TTCTCTGATG TTCCTCAAGG CCTTCTCACC 
CCTTCCAGGG CCAGTTTAAA TCTTGACCTG TATGAGCATC ACTGGCCAGG AGCTCAAGGT 
CCTGAGGGGA CTCCTCCAGC CAGGCAGCCA CAAGGGCCCT TCAGAGGGGA GGTACGCGTC 
CCCAGTCGGA CCAATTCTTT CAACAACCAC CAAAAGGTGA CTGTGAGGCA GACATTGCCC 
CCAACAGCTA GTGTTGCTGG GAAGCCAGAG TCATCTTTAG GCCCACCTAA CACAATAACT 
GCAGTGACCT CTCCACCAAT TCAGCCACCT GTTAAGAGTA TACGGGTCAT GAGGCCTGAG 
CCGAAGACAG CAGTGGGACC ATGCCATCCA GGGTGGCTGA GTGCACAATC CCCGGAAGCA 
CAGGATTCTC TTGGATACAT CCCAGATGAG AATTTTACCC TGGAACCCAG TCAAGAACAT 
CGATGCCCAC CTCCACCTTA CCCCAAAACA CTGCTTGTGT CTGGATCTGG ACCTGAGCCT 
GCATCCTTAG AGCCTGTAGC CATGAGTGGA CTTCAAGATG TCAGTGCCAC AGGTAGGACT 
ATCCAGAGTT CCTCGGTAGG AAAGCAGGAG GAACCCATCT TCAAAGACAA AGTGAAAATG 
GGCAAAGGAG AAAAGGCTGT GAAGGATAAG AAGCAGATCC AGACATCACC TGTGCCTGTA 
CGAAAAAATG CCCGTGATGA AGAGAAGAGG GAGTCTCGTA TTAAGAGTTA TTCTCCATTT 
GCCTTTAAGT TCTACATGGA GCAACATGTG GAGAATGTGA TGAAGACATA CCAGCAGAAA 
CTTAATCGCA GAATGCAGCT GGAGCAAGAG ATGTCCAAGG CTGGCCTTTC AGAGGCTGAG 
CAGGAACAGA TGAGAAAGAT GCTTTACCAG AAGGAATCCA ATTACAATCG ACTACGACGT 
GCCAAGATGG ACAAATCAAT GTTTGTCAAA ATCAAGACAC TCGGCATTGG GGCCTTTGGT 
GAGGTGTGTC TAACCCGAAA AGTAGACACA GGAGCCTTGT ATGCAATGAA GACTTTGCGA 
AAGAAAGACG TTCTTAACCG AAATCAAGTA GCCCATGTTA AAGCTGAACG GGACATTTTA 
GCAGAGGCTG ACAATGAGTG GGTAGTCAGG TTGTACTATT CATTTCAAGA CCGAGACAAT 
CTGTATTTTG TCATGGATTA CATTCCTGGA GGAGATATGA TGAGCTTGCT GATCCGTATG 
GGAGTTTTTC CAGAGGTCCT AGCCAGGTTT TATGTGGCAG AACTGACACT GGCAATTGAG 
AGTGTGCATA AGATGGGTTT CATTCACAGA GATATCAAGC CAGACAATAT CCTCATTGAC 
CTGGATGGAC ACATCAAACT GACTGATTTT GGTCTATGTA CAGGGTTCCG ATGGACTCAC 
AACTCAAAGT ATTACCAAAA AGGGAACCAT ATCCGACAGG ATAGCATGGA GCCAAGTGAC 
TCTTGGGATG ATGTGTCTAA TTGCCGTTGT GGTGACCGGC TGATGACTTT GGAACAGAGA 
GCAACTCGAC AACACCAGCG CTGCCTGGCG CATTCTTTGG TGGGCACCCC TAACTACATT 
GCCCCTGAGG TGCTTTTGCG CAAAGGATAC ACACAGCTTT GCGACTGGTG GAGTGTGGGA 
GTGATCCTGT TTGAGATGCT GGTTGGCCAA CCACCCTTTC TTGCTCCAAC CCCAACAGAG 
GCACAGCTTA AGGTGATAAA CTGGGAGAAC ACTTTGCAAG TGCCACCACA AGTAAAACTA 
AGCCCAGAGG CAGTCGATAT TATTGGCCAG CTTTGCTGCT CTGCTGAAGA GCGTCTAGGA 
GGCAATGGGG CTGGAGAAAT AAAGGCTCAT CCTTTTTTTT CCGAGGTGGA TTTCTCCAGC 
AATCTTCGGA CACAACCTGC ACCATATCGA CCAAAAATTG CCCACCCCAT GGATACATCC 
AACTTTGATC CTGTTGAAGA GGAGGGAGGC CCAGGCGCCT GGAGTGATAG TGGGGATAGC 
ACCCGCACTT GGGACACCCT CTGTTCTCCT CACGGCAAAC ACCCTGAACA TGCCTTCTAT 
GAGTTTACTT TCCGAAGATT TTTTGATGAT AATGGTTGTC CATTCCGTTA TCCCAAACCC 
CTAGAGGCGA CACAAAGCCT GTCCAGCAGT GACGGGGCCG GCAGCCTGAA GCCTGAGCTG 
GAGGAGGAAG AGGCCCATGA AGAGGAAGAG CAAGGGGAAG GGTGTGAGCC GGTTTATGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116779.1
CDS	248..3670
Translation	MRPKTFPATPYVGNTRQRLQDIKEGLKQPAKLVSQALHGGSSRGEGGRGGDSKGKDQAARQQQLRPPQKFNNYQSALREIRKSLRPYANDSGPGSSSGHPGEVSRQMLQDLVNAGCDQEMAVRALKQTGSRNIEAALEYISKMSYLDPHTEQIVRVIKQTSPGKSGMPSLMDHRTAMEGSGDGALPPYHQMGAPLYEGASYGAEGEMARAYMGGPPVMNYMPPPANPSQGVTMGNPMGRPASMGAYSTNMAPQNNTGNPMYTPGPAQQKGYPSGIDQAMMGYSVSGPPMQIQSQPSGGPGAHYDYRSHMMENSSYGVKRSASFQNKMPQLTTENYVNMPGKAAMGQNAGGYAPNLYLPPHSHPRQASPTSHQVHMMQRPPGVAPGAMGGEFSDVPQGLLTPSRASLNLDLYEHHWPGAQGPEGTPPARQPQGPFRGEVRVPSRTNSFNNHQKVTVRQTLPPTASVAGKPESSLGPPNTITAVTSPPIQPPVKSIRVMRPEPKTAVGPCHPGWLSAQSPEAQDSLGYIPDENFTLEPSQEHRCPPPPYPKTLLVSGSGPEPASLEPVAMSGLQDVSATGRTIQSSSVGKQEEPIFKDKVKMGKGEKAVKDKKQIQTSPVPVRKNARDEEKRESRIKSYSPFAFKFYMEQHVENVMKTYQQKLNRRMQLEQEMSKAGLSEAEQEQMRKMLYQKESNYNRLRRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTGALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRDNLYFVMDYIPGGDMMSLLIRMGVFPEVLARFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGNHIRQDSMEPSDSWDDVSNCRCGDRLMTLEQRATRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTEAQLKVINWENTLQVPPQVKLSPEAVDIIGQLCCSAEERLGGNGAGEIKAHPFFSEVDFSSNLRTQPAPYRPKIAHPMDTSNFDPVEEEGGPGAWSDSGDSTRTWDTLCSPHGKHPEHAFYEFTFRRFFDDNGCPFRYPKPLEATQSLSSSDGAGSLKPELEEEEAHEEEEQGEGCEPVYV

Target ORF information:

RefSeq Version	NM_001123307.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123307.1

ORF Insert Sequence:

ATGAGGCCGA AGACGTTCCC TGCCACTCCA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCGCT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAGTTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAGAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT GTTACCATGG GAAACCCGAT GGGACGCCCT 
GCGAGTATGG GTGCTTACTC CACAAACATG GCTCCTCAAA ATAATACTGG GAATCCAATG 
TACACCCCTG GTCCAGCCCA GCAAAAAGGA TACCCTAGTG GGATTGACCA GGCCATGATG 
GGGTACAGTG TGTCTGGACC ACCCATGCAA ATCCAGTCTC AGCCCTCAGG AGGGCCTGGA 
GCACATTATG ACTACAGGTC GCATATGATG GAGAATTCCA GCTACGGGGT CAAAAGAAGT 
GCTTCCTTTC AGAATAAGAT GCCCCAACTA ACTACAGAAA ATTATGTCAA TATGCCAGGC 
AAAGCAGCAA TGGGCCAAAA TGCTGGTGGC TATGCTCCCA ACTTATATCT GCCACCACAT 
TCACATCCAC GCCAAGCTAG CCCCACCTCA CACCAGGTTC ACATGATGCA GCGCCCCCCA 
GGTGTTGCCC CTGGAGCTAT GGGTGGGGAG TTCTCTGATG TTCCTCAAGG CCTTCTCACC 
CCTTCCAGGG CCAGTTTAAA TCTTGACCTG TATGAGCATC ACTGGCCAGG AGCTCAAGGT 
CCTGAGGGGA CTCCTCCAGC CAGGCAGCCA CAAGGGCCCT TCAGAGGGGA GGTACGCGTC 
CCCAGTCGGA CCAATTCTTT CAACAACCAC CAAAAGGTGA CTGTGAGGCA GACATTGCCC 
CCAACAGCTA GTGTTGCTGG GAAGCCAGAG TCATCTTTAG GCCCACCTAA CACAATAACT 
GCAGTGACCT CTCCACCAAT TCAGCCACCT GTTAAGAGTA TACGGGTCAT GAGGCCTGAG 
CCGAAGACAG CAGTGGGACC ATGCCATCCA GGGTGGCTGA GTGCACAATC CCCGGAAGCA 
CAGGATTCTC TTGGATACAT CCCAGATGAG AATTTTACCC TGGAACCCAG TCAAGAACAT 
CGATGCCCAC CTCCACCTTA CCCCAAAACA CTGCTTGTGT CTGGATCTGG ACCTGAGCCT 
GCATCCTTAG AGCCTGTAGC CATGAGTGGA CTTCAAGATG TCAGTGCCAC AGGTAGGACT 
ATCCAGAGTT CCTCGGTAGG AAAGCAGGAG GAACCCATCT TCAAAGACAA AGTGAAAATG 
GGCAAAGGAG AAAAGGCTGT GAAGGATAAG AAGCAGATCC AGACATCACC TGTGCCTGTA 
CGAAAAAATG CCCGTGATGA AGAGAAGAGG GAGTCTCGTA TTAAGAGTTA TTCTCCATTT 
GCCTTTAAGT TCTACATGGA GCAACATGTG GAGAATGTGA TGAAGACATA CCAGCAGAAA 
CTTAATCGCA GAATGCAGCT GGAGCAAGAG ATGTCCAAGG CTGGCCTTTC AGAGGCTGAG 
CAGGAACAGA TGAGAAAGAT GCTTTACCAG AAGGAATCCA ATTACAATCG ACTACGACGT 
GCCAAGATGG ACAAATCAAT GTTTGTCAAA ATCAAGACAC TCGGCATTGG GGCCTTTGGT 
GAGGTGTGTC TAACCCGAAA AGTAGACACA GGAGCCTTGT ATGCAATGAA GACTTTGCGA 
AAGAAAGACG TTCTTAACCG AAATCAAGTA GCCCATGTTA AAGCTGAACG GGACATTTTA 
GCAGAGGCTG ACAATGAGTG GGTAGTCAGG TTGTACTATT CATTTCAAGA CCGAGACAAT 
CTGTATTTTG TCATGGATTA CATTCCTGGA GGAGATATGA TGAGCTTGCT GATCCGTATG 
GGAGTTTTTC CAGAGGTCCT AGCCAGGTTT TATGTGGCAG AACTGACACT GGCAATTGAG 
AGTGTGCATA AGATGGGTTT CATTCACAGA GATATCAAGC CAGACAATAT CCTCATTGAC 
CTGGATGGAC ACATCAAACT GACTGATTTT GGTCTATGTA CAGGGTTCCG ATGGACTCAC 
AACTCAAAGT ATTACCAAAA AGGGAACCAT ATCCGACAGG ATAGCATGGA GCCAAGTGAC 
TCTTGGGATG ATGTGTCTAA TTGCCGTTGT GGTGACCGGC TGATGACTTT GGAACAGAGA 
GCAACTCGAC AACACCAGCG CTGCCTGGCG CATTCTTTGG TGGGCACCCC TAACTACATT 
GCCCCTGAGG TGCTTTTGCG CAAAGGATAC ACACAGCTTT GCGACTGGTG GAGTGTGGGA 
GTGATCCTGT TTGAGATGCT GGTTGGCCAA CCACCCTTTC TTGCTCCAAC CCCAACAGAG 
GCACAGCTTA AGGTGATAAA CTGGGAGAAC ACTTTGCAAG TGCCACCACA AGTAAAACTA 
AGCCCAGAGG CAGTCGATAT TATTGGCCAG CTTTGCTGCT CTGCTGAAGA GCGTCTAGGA 
GGCAATGGGG CTGGAGAAAT AAAGGCTCAT CCTTTTTTTT CCGAGGTGGA TTTCTCCAGC 
AATCTTCGGA CACAACCTGC ACCATATCGA CCAAAAATTG CCCACCCCAT GGATACATCC 
AACTTTGATC CTGTTGAAGA GGAGGGAGGC CCAGGCGCCT GGAGTGATAG TGGGGATAGC 
ACCCGCACTT GGGACACCCT CTGTTCTCCT CACGGCAAAC ACCCTGAACA TGCCTTCTAT 
GAGTTTACTT TCCGAAGATT TTTTGATGAT AATGGTTGTC CATTCCGTTA TCCCAAACCC 
CTAGAGGCGA CACAAAGCCT GTCCAGCAGT GACGGGGCCG GCAGCCTGAA GCCTGAGCTG 
GAGGAGGAAG AGGCCCATGA AGAGGAAGAG CAAGGGGAAG GGTGTGAGCC GGTTTATGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats2 ( XM_005167579.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167636.1 Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15095
Clone ID Related Accession (Same CDS sequence)	XM_005167579.3 , XM_005167579.4 , XM_021478741.1
Accession Version	XM_005167579.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase LATS2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCGA AGACGTTCCC TGCCACTCCA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCACT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAATTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAAAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT GCTACCATGG GAAACCCGAT GGGACGCCCT 
GCAAGTATGG GTGCTTACTC CACAAACATG GCTCCTCAAA ATAATACTGG GAATCCAATG 
TACACCCCTG GTCCAGCCCA GCAAAAAGGA TACCCTAGTG GGATTGACCA GGCCATGATG 
GGGTACAGTG TGTCTGGACC ACCCATGCAA ATCCAGTCTC AGCCCTCAGG AGGGCCTGGA 
GCACATTATG ACTACAGGTC GCATATGATG GAGAATTCCA GCTACGGGGT CAAAAGAAGT 
GCTTCCTTTC AGAATAAGAT GCCCCAACTA ACTACAGAAA ATTATGTCAA TATGCCAAGC 
AAAGCAGCAA TGGGCCAAAA TGCTGGTGGC TATGCTCCCA ACTTATATCT GCCACCACAT 
TCACATCCAC GCCAAGCTAG CCCCACCTCA CACCAGGTTC ACATGATGCA GCGCCCCCCA 
GGTGTTGCCC CTGGAGCTAT GGGTGGGGAG TTCTCTGATG TTCCTCAAGG CCTTCTCACC 
CCTTCCAGGG CCAGTTTAAA TCTTGACCTG TATGAGCATC ACTGGCCAGG AGCTCAAGGT 
CCTGAGGGGA CTCCTCCAGC CAGGCAGCCA CAAGGGCCCT TCAGAGGGGA GGTACGCGTC 
CCCAGTCGGA CCAATTCTTT CAACAACCAC CAAAAGGTGA CTGTGAGGCA GACATTGCCC 
CCAACAGCTA GTGTTGCTGG GAAGCCAGAG TCATCTTTGG GCCCACCTAA CACAATAACT 
GCAGTGACCT CTCCACCAAT TCAGCCACCT GTTAAGAGTA TACGGGTCAT GAGGCCTGAG 
CCGAAGACAG CAGTGGGACC ATGCCATCCA GGGTGGCTGA GTGCACAATC CCCGGAAGCA 
CAGGATTCTC TTGGATACAT CCCAGATGAG AATTTTACCC TGGAACCCAG TCAAGAACAT 
CGATGCCCAC CTCCACCTTA CCCCAAAACA CTGCTTGTGT CTGGATCTGG ACCTGAGCCT 
GCATCCTTAG AGCCTGTAGC CATGAGTGGA CTTCAAGATG TCAGTGCCAC AGGTAGGACT 
ATCCAGAGTT CCTCGGTAGG AAAGCAGGAG GAATCCATCT TCAAAGACAA AGTGAAAATG 
GGCAAAGGAG AAAAGGCTGT GAAGGATAAG AAGCAGATCC AGACATCACC TGTGCCTGTA 
CGAAAAAATG CCCGTGATGA AGAGAAGAGG GAGTCTCGTA TTAAGAGTTA TTCTCCATTT 
GCCTTTAAGT TCTACATGGA GCAACATGTG GAGAATGTGA TGAAGACATA CCAGCAGAAA 
CTTAATCGCA GAATGCAGCT GGAGCAAGAG ATGTCCAAGG CTGGCCTTTC AGAGGCTGAG 
CAGGAACAGA TGAGAAAGAT GCTTTACCAG AAGGAATCCA ATTACAATCG ACTACGACGT 
GCCAAGATGG ACAAATCAAT GTTTGTCAAA ATCAAGACAC TCGGCATTGG GGCCTTTGGT 
GAGGTGTGTC TAACCCGAAA AGTAGACACA GGAGCCTTGT ATGCAATGAA GACTTTGCGA 
AAGAAAGACG TTCTTAACCG AAATCAAGTA GCCCATGTTA AAGCTGAACG GGACATTTTA 
GCAGAGGCTG ACAATGAGTG GGTAGTCAGG TTGTACTATT CATTTCAAGA CCGAGACAAT 
CTGTATTTTG TCATGGATTA CATTCCTGGA GGAGATATGA TGAGCTTGCT GATCCGTATG 
GGAGTTTTTC CAGAGGTCTT AGCCAGGTTT TATGTGGCAG AACTGACACT GGCAATTGAG 
AGTGTGCATA AGATGGGTTT CATCCACAGA GATATCAAGC CAGACAATAT CCTCATTGAC 
CTGGATGGAC ACATCAAACT GACTGATTTT GGTCTATGTA CGGGGTTCCG ATGGACTCAC 
AACTCAAAGT ATTACCAAAA AGGGAACCAT ATCCGACAGG ATAGCATGGA GCCAAGTGAC 
TTTTGGGATG ATGTGTCTAA TTGCCGTTGT GGTGACCGGC TGATGACTTT GGAACAGAGA 
GCAACTCGAC AACACCAGCG CTGCCTGGCG CATTCTTTGG TGGGCACCCC TAACTACATT 
GCCCCTGAGG TGCTTTTGCG CAAAGGATAC ACACAGCTGT GCGACTGGTG GAGTGTGGGA 
GTGATCCTGT TTGAGATGCT GGTTGGCCAA CCACCCTTTC TTGCTCCAAC CCCAACAGAG 
ACGCAGCTTA AGGTGATAAA CTGGGAGAAC ACTTTGCAAG TGCCACCACA AGTAAAACTA 
AGCCCAGAGG CAGTCGATAT TATTGGCCAG CTTTGCTGCT CTGCTGAAGA GCGTCTAGGA 
GGCAATGGGG CTGGAGAAAT AAAGGCTCAT CCTTTTTTTT CCGAGGTGGA TTTCTCCAGC 
AATCTTCGGA CACAACCTGC ACCATATCGA CCAAAAATTG CCCACCCCAT GGATACATCC 
AACTTTGATC CTGTTGAAGA GGAGGGAGGC CCAGGCGCCT GGAGTGATAG TGGGGATAGC 
ACCCGCACTT GGGACACCCT CTGTTCTCCT CACGGCAAAC ACCCTGAACA TGCCTTCTAT 
GAGTTTACTT TCCGAAGATT TTTTGATGAT AATGGTTGTC CATTCCGTTA TCCCAAACCC 
CTAGAGGCGA CACAAAGCCT GTCCAGCAGT GACGGGGCCG GCAGCCTGAA GCCTGAGCTG 
GAGGAGGAAG AGGCCCATGA AGAGGAAGAG CAAGGGGAAG GGTGTGAGCC GGTTTATGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167636.1
CDS	469..3891
Translation	MRPKTFPATPYVGNTRQRLQDIKEGLKQPAKLVSQALHGGSSRGEGGRGGDSKGKDQAARQQQLRPPQKFNNYQSALREIRKSLRPYANDSGPGSSSGHPGEVSRQMLQDLVNAGCDQEMAVRALKQTGSRNIEAALEYISKMSYLDPHTEQIVRVIKQTSPGKSGMPNLMDHRTAMEGSGDGALPPYHQMGAPLYEGASYGAEGEMARAYMGGPPVMNYMPPPANPSQGATMGNPMGRPASMGAYSTNMAPQNNTGNPMYTPGPAQQKGYPSGIDQAMMGYSVSGPPMQIQSQPSGGPGAHYDYRSHMMENSSYGVKRSASFQNKMPQLTTENYVNMPSKAAMGQNAGGYAPNLYLPPHSHPRQASPTSHQVHMMQRPPGVAPGAMGGEFSDVPQGLLTPSRASLNLDLYEHHWPGAQGPEGTPPARQPQGPFRGEVRVPSRTNSFNNHQKVTVRQTLPPTASVAGKPESSLGPPNTITAVTSPPIQPPVKSIRVMRPEPKTAVGPCHPGWLSAQSPEAQDSLGYIPDENFTLEPSQEHRCPPPPYPKTLLVSGSGPEPASLEPVAMSGLQDVSATGRTIQSSSVGKQEESIFKDKVKMGKGEKAVKDKKQIQTSPVPVRKNARDEEKRESRIKSYSPFAFKFYMEQHVENVMKTYQQKLNRRMQLEQEMSKAGLSEAEQEQMRKMLYQKESNYNRLRRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTGALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRDNLYFVMDYIPGGDMMSLLIRMGVFPEVLARFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGNHIRQDSMEPSDFWDDVSNCRCGDRLMTLEQRATRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLQVPPQVKLSPEAVDIIGQLCCSAEERLGGNGAGEIKAHPFFSEVDFSSNLRTQPAPYRPKIAHPMDTSNFDPVEEEGGPGAWSDSGDSTRTWDTLCSPHGKHPEHAFYEFTFRRFFDDNGCPFRYPKPLEATQSLSSSDGAGSLKPELEEEEAHEEEEQGEGCEPVYV

Target ORF information:

RefSeq Version	XM_005167579.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167579.3

ORF Insert Sequence:

ATGAGGCCGA AGACGTTCCC TGCCACTCCA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCACT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAATTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAAAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT GCTACCATGG GAAACCCGAT GGGACGCCCT 
GCAAGTATGG GTGCTTACTC CACAAACATG GCTCCTCAAA ATAATACTGG GAATCCAATG 
TACACCCCTG GTCCAGCCCA GCAAAAAGGA TACCCTAGTG GGATTGACCA GGCCATGATG 
GGGTACAGTG TGTCTGGACC ACCCATGCAA ATCCAGTCTC AGCCCTCAGG AGGGCCTGGA 
GCACATTATG ACTACAGGTC GCATATGATG GAGAATTCCA GCTACGGGGT CAAAAGAAGT 
GCTTCCTTTC AGAATAAGAT GCCCCAACTA ACTACAGAAA ATTATGTCAA TATGCCAAGC 
AAAGCAGCAA TGGGCCAAAA TGCTGGTGGC TATGCTCCCA ACTTATATCT GCCACCACAT 
TCACATCCAC GCCAAGCTAG CCCCACCTCA CACCAGGTTC ACATGATGCA GCGCCCCCCA 
GGTGTTGCCC CTGGAGCTAT GGGTGGGGAG TTCTCTGATG TTCCTCAAGG CCTTCTCACC 
CCTTCCAGGG CCAGTTTAAA TCTTGACCTG TATGAGCATC ACTGGCCAGG AGCTCAAGGT 
CCTGAGGGGA CTCCTCCAGC CAGGCAGCCA CAAGGGCCCT TCAGAGGGGA GGTACGCGTC 
CCCAGTCGGA CCAATTCTTT CAACAACCAC CAAAAGGTGA CTGTGAGGCA GACATTGCCC 
CCAACAGCTA GTGTTGCTGG GAAGCCAGAG TCATCTTTGG GCCCACCTAA CACAATAACT 
GCAGTGACCT CTCCACCAAT TCAGCCACCT GTTAAGAGTA TACGGGTCAT GAGGCCTGAG 
CCGAAGACAG CAGTGGGACC ATGCCATCCA GGGTGGCTGA GTGCACAATC CCCGGAAGCA 
CAGGATTCTC TTGGATACAT CCCAGATGAG AATTTTACCC TGGAACCCAG TCAAGAACAT 
CGATGCCCAC CTCCACCTTA CCCCAAAACA CTGCTTGTGT CTGGATCTGG ACCTGAGCCT 
GCATCCTTAG AGCCTGTAGC CATGAGTGGA CTTCAAGATG TCAGTGCCAC AGGTAGGACT 
ATCCAGAGTT CCTCGGTAGG AAAGCAGGAG GAATCCATCT TCAAAGACAA AGTGAAAATG 
GGCAAAGGAG AAAAGGCTGT GAAGGATAAG AAGCAGATCC AGACATCACC TGTGCCTGTA 
CGAAAAAATG CCCGTGATGA AGAGAAGAGG GAGTCTCGTA TTAAGAGTTA TTCTCCATTT 
GCCTTTAAGT TCTACATGGA GCAACATGTG GAGAATGTGA TGAAGACATA CCAGCAGAAA 
CTTAATCGCA GAATGCAGCT GGAGCAAGAG ATGTCCAAGG CTGGCCTTTC AGAGGCTGAG 
CAGGAACAGA TGAGAAAGAT GCTTTACCAG AAGGAATCCA ATTACAATCG ACTACGACGT 
GCCAAGATGG ACAAATCAAT GTTTGTCAAA ATCAAGACAC TCGGCATTGG GGCCTTTGGT 
GAGGTGTGTC TAACCCGAAA AGTAGACACA GGAGCCTTGT ATGCAATGAA GACTTTGCGA 
AAGAAAGACG TTCTTAACCG AAATCAAGTA GCCCATGTTA AAGCTGAACG GGACATTTTA 
GCAGAGGCTG ACAATGAGTG GGTAGTCAGG TTGTACTATT CATTTCAAGA CCGAGACAAT 
CTGTATTTTG TCATGGATTA CATTCCTGGA GGAGATATGA TGAGCTTGCT GATCCGTATG 
GGAGTTTTTC CAGAGGTCTT AGCCAGGTTT TATGTGGCAG AACTGACACT GGCAATTGAG 
AGTGTGCATA AGATGGGTTT CATCCACAGA GATATCAAGC CAGACAATAT CCTCATTGAC 
CTGGATGGAC ACATCAAACT GACTGATTTT GGTCTATGTA CGGGGTTCCG ATGGACTCAC 
AACTCAAAGT ATTACCAAAA AGGGAACCAT ATCCGACAGG ATAGCATGGA GCCAAGTGAC 
TTTTGGGATG ATGTGTCTAA TTGCCGTTGT GGTGACCGGC TGATGACTTT GGAACAGAGA 
GCAACTCGAC AACACCAGCG CTGCCTGGCG CATTCTTTGG TGGGCACCCC TAACTACATT 
GCCCCTGAGG TGCTTTTGCG CAAAGGATAC ACACAGCTGT GCGACTGGTG GAGTGTGGGA 
GTGATCCTGT TTGAGATGCT GGTTGGCCAA CCACCCTTTC TTGCTCCAAC CCCAACAGAG 
ACGCAGCTTA AGGTGATAAA CTGGGAGAAC ACTTTGCAAG TGCCACCACA AGTAAAACTA 
AGCCCAGAGG CAGTCGATAT TATTGGCCAG CTTTGCTGCT CTGCTGAAGA GCGTCTAGGA 
GGCAATGGGG CTGGAGAAAT AAAGGCTCAT CCTTTTTTTT CCGAGGTGGA TTTCTCCAGC 
AATCTTCGGA CACAACCTGC ACCATATCGA CCAAAAATTG CCCACCCCAT GGATACATCC 
AACTTTGATC CTGTTGAAGA GGAGGGAGGC CCAGGCGCCT GGAGTGATAG TGGGGATAGC 
ACCCGCACTT GGGACACCCT CTGTTCTCCT CACGGCAAAC ACCCTGAACA TGCCTTCTAT 
GAGTTTACTT TCCGAAGATT TTTTGATGAT AATGGTTGTC CATTCCGTTA TCCCAAACCC 
CTAGAGGCGA CACAAAGCCT GTCCAGCAGT GACGGGGCCG GCAGCCTGAA GCCTGAGCTG 
GAGGAGGAAG AGGCCCATGA AGAGGAAGAG CAAGGGGAAG GGTGTGAGCC GGTTTATGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats2 ( XM_005167579.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167636.1 Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15095
Clone ID Related Accession (Same CDS sequence)	XM_005167579.3 , XM_005167579.4 , XM_021478741.1
Accession Version	XM_005167579.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase LATS2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167579.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCGA AGACGTTCCC TGCCACTCCA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCACT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAATTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAAAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT GCTACCATGG GAAACCCGAT GGGACGCCCT 
GCAAGTATGG GTGCTTACTC CACAAACATG GCTCCTCAAA ATAATACTGG GAATCCAATG 
TACACCCCTG GTCCAGCCCA GCAAAAAGGA TACCCTAGTG GGATTGACCA GGCCATGATG 
GGGTACAGTG TGTCTGGACC ACCCATGCAA ATCCAGTCTC AGCCCTCAGG AGGGCCTGGA 
GCACATTATG ACTACAGGTC GCATATGATG GAGAATTCCA GCTACGGGGT CAAAAGAAGT 
GCTTCCTTTC AGAATAAGAT GCCCCAACTA ACTACAGAAA ATTATGTCAA TATGCCAAGC 
AAAGCAGCAA TGGGCCAAAA TGCTGGTGGC TATGCTCCCA ACTTATATCT GCCACCACAT 
TCACATCCAC GCCAAGCTAG CCCCACCTCA CACCAGGTTC ACATGATGCA GCGCCCCCCA 
GGTGTTGCCC CTGGAGCTAT GGGTGGGGAG TTCTCTGATG TTCCTCAAGG CCTTCTCACC 
CCTTCCAGGG CCAGTTTAAA TCTTGACCTG TATGAGCATC ACTGGCCAGG AGCTCAAGGT 
CCTGAGGGGA CTCCTCCAGC CAGGCAGCCA CAAGGGCCCT TCAGAGGGGA GGTACGCGTC 
CCCAGTCGGA CCAATTCTTT CAACAACCAC CAAAAGGTGA CTGTGAGGCA GACATTGCCC 
CCAACAGCTA GTGTTGCTGG GAAGCCAGAG TCATCTTTGG GCCCACCTAA CACAATAACT 
GCAGTGACCT CTCCACCAAT TCAGCCACCT GTTAAGAGTA TACGGGTCAT GAGGCCTGAG 
CCGAAGACAG CAGTGGGACC ATGCCATCCA GGGTGGCTGA GTGCACAATC CCCGGAAGCA 
CAGGATTCTC TTGGATACAT CCCAGATGAG AATTTTACCC TGGAACCCAG TCAAGAACAT 
CGATGCCCAC CTCCACCTTA CCCCAAAACA CTGCTTGTGT CTGGATCTGG ACCTGAGCCT 
GCATCCTTAG AGCCTGTAGC CATGAGTGGA CTTCAAGATG TCAGTGCCAC AGGTAGGACT 
ATCCAGAGTT CCTCGGTAGG AAAGCAGGAG GAATCCATCT TCAAAGACAA AGTGAAAATG 
GGCAAAGGAG AAAAGGCTGT GAAGGATAAG AAGCAGATCC AGACATCACC TGTGCCTGTA 
CGAAAAAATG CCCGTGATGA AGAGAAGAGG GAGTCTCGTA TTAAGAGTTA TTCTCCATTT 
GCCTTTAAGT TCTACATGGA GCAACATGTG GAGAATGTGA TGAAGACATA CCAGCAGAAA 
CTTAATCGCA GAATGCAGCT GGAGCAAGAG ATGTCCAAGG CTGGCCTTTC AGAGGCTGAG 
CAGGAACAGA TGAGAAAGAT GCTTTACCAG AAGGAATCCA ATTACAATCG ACTACGACGT 
GCCAAGATGG ACAAATCAAT GTTTGTCAAA ATCAAGACAC TCGGCATTGG GGCCTTTGGT 
GAGGTGTGTC TAACCCGAAA AGTAGACACA GGAGCCTTGT ATGCAATGAA GACTTTGCGA 
AAGAAAGACG TTCTTAACCG AAATCAAGTA GCCCATGTTA AAGCTGAACG GGACATTTTA 
GCAGAGGCTG ACAATGAGTG GGTAGTCAGG TTGTACTATT CATTTCAAGA CCGAGACAAT 
CTGTATTTTG TCATGGATTA CATTCCTGGA GGAGATATGA TGAGCTTGCT GATCCGTATG 
GGAGTTTTTC CAGAGGTCTT AGCCAGGTTT TATGTGGCAG AACTGACACT GGCAATTGAG 
AGTGTGCATA AGATGGGTTT CATCCACAGA GATATCAAGC CAGACAATAT CCTCATTGAC 
CTGGATGGAC ACATCAAACT GACTGATTTT GGTCTATGTA CGGGGTTCCG ATGGACTCAC 
AACTCAAAGT ATTACCAAAA AGGGAACCAT ATCCGACAGG ATAGCATGGA GCCAAGTGAC 
TTTTGGGATG ATGTGTCTAA TTGCCGTTGT GGTGACCGGC TGATGACTTT GGAACAGAGA 
GCAACTCGAC AACACCAGCG CTGCCTGGCG CATTCTTTGG TGGGCACCCC TAACTACATT 
GCCCCTGAGG TGCTTTTGCG CAAAGGATAC ACACAGCTGT GCGACTGGTG GAGTGTGGGA 
GTGATCCTGT TTGAGATGCT GGTTGGCCAA CCACCCTTTC TTGCTCCAAC CCCAACAGAG 
ACGCAGCTTA AGGTGATAAA CTGGGAGAAC ACTTTGCAAG TGCCACCACA AGTAAAACTA 
AGCCCAGAGG CAGTCGATAT TATTGGCCAG CTTTGCTGCT CTGCTGAAGA GCGTCTAGGA 
GGCAATGGGG CTGGAGAAAT AAAGGCTCAT CCTTTTTTTT CCGAGGTGGA TTTCTCCAGC 
AATCTTCGGA CACAACCTGC ACCATATCGA CCAAAAATTG CCCACCCCAT GGATACATCC 
AACTTTGATC CTGTTGAAGA GGAGGGAGGC CCAGGCGCCT GGAGTGATAG TGGGGATAGC 
ACCCGCACTT GGGACACCCT CTGTTCTCCT CACGGCAAAC ACCCTGAACA TGCCTTCTAT 
GAGTTTACTT TCCGAAGATT TTTTGATGAT AATGGTTGTC CATTCCGTTA TCCCAAACCC 
CTAGAGGCGA CACAAAGCCT GTCCAGCAGT GACGGGGCCG GCAGCCTGAA GCCTGAGCTG 
GAGGAGGAAG AGGCCCATGA AGAGGAAGAG CAAGGGGAAG GGTGTGAGCC GGTTTATGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167636.1
CDS	601..4023
Translation	MRPKTFPATPYVGNTRQRLQDIKEGLKQPAKLVSQALHGGSSRGEGGRGGDSKGKDQAARQQQLRPPQKFNNYQSALREIRKSLRPYANDSGPGSSSGHPGEVSRQMLQDLVNAGCDQEMAVRALKQTGSRNIEAALEYISKMSYLDPHTEQIVRVIKQTSPGKSGMPNLMDHRTAMEGSGDGALPPYHQMGAPLYEGASYGAEGEMARAYMGGPPVMNYMPPPANPSQGATMGNPMGRPASMGAYSTNMAPQNNTGNPMYTPGPAQQKGYPSGIDQAMMGYSVSGPPMQIQSQPSGGPGAHYDYRSHMMENSSYGVKRSASFQNKMPQLTTENYVNMPSKAAMGQNAGGYAPNLYLPPHSHPRQASPTSHQVHMMQRPPGVAPGAMGGEFSDVPQGLLTPSRASLNLDLYEHHWPGAQGPEGTPPARQPQGPFRGEVRVPSRTNSFNNHQKVTVRQTLPPTASVAGKPESSLGPPNTITAVTSPPIQPPVKSIRVMRPEPKTAVGPCHPGWLSAQSPEAQDSLGYIPDENFTLEPSQEHRCPPPPYPKTLLVSGSGPEPASLEPVAMSGLQDVSATGRTIQSSSVGKQEESIFKDKVKMGKGEKAVKDKKQIQTSPVPVRKNARDEEKRESRIKSYSPFAFKFYMEQHVENVMKTYQQKLNRRMQLEQEMSKAGLSEAEQEQMRKMLYQKESNYNRLRRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTGALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRDNLYFVMDYIPGGDMMSLLIRMGVFPEVLARFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGNHIRQDSMEPSDFWDDVSNCRCGDRLMTLEQRATRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLQVPPQVKLSPEAVDIIGQLCCSAEERLGGNGAGEIKAHPFFSEVDFSSNLRTQPAPYRPKIAHPMDTSNFDPVEEEGGPGAWSDSGDSTRTWDTLCSPHGKHPEHAFYEFTFRRFFDDNGCPFRYPKPLEATQSLSSSDGAGSLKPELEEEEAHEEEEQGEGCEPVYV

Target ORF information:

RefSeq Version	XM_005167579.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167579.4

ORF Insert Sequence:

ATGAGGCCGA AGACGTTCCC TGCCACTCCA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCACT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAATTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAAAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT GCTACCATGG GAAACCCGAT GGGACGCCCT 
GCAAGTATGG GTGCTTACTC CACAAACATG GCTCCTCAAA ATAATACTGG GAATCCAATG 
TACACCCCTG GTCCAGCCCA GCAAAAAGGA TACCCTAGTG GGATTGACCA GGCCATGATG 
GGGTACAGTG TGTCTGGACC ACCCATGCAA ATCCAGTCTC AGCCCTCAGG AGGGCCTGGA 
GCACATTATG ACTACAGGTC GCATATGATG GAGAATTCCA GCTACGGGGT CAAAAGAAGT 
GCTTCCTTTC AGAATAAGAT GCCCCAACTA ACTACAGAAA ATTATGTCAA TATGCCAAGC 
AAAGCAGCAA TGGGCCAAAA TGCTGGTGGC TATGCTCCCA ACTTATATCT GCCACCACAT 
TCACATCCAC GCCAAGCTAG CCCCACCTCA CACCAGGTTC ACATGATGCA GCGCCCCCCA 
GGTGTTGCCC CTGGAGCTAT GGGTGGGGAG TTCTCTGATG TTCCTCAAGG CCTTCTCACC 
CCTTCCAGGG CCAGTTTAAA TCTTGACCTG TATGAGCATC ACTGGCCAGG AGCTCAAGGT 
CCTGAGGGGA CTCCTCCAGC CAGGCAGCCA CAAGGGCCCT TCAGAGGGGA GGTACGCGTC 
CCCAGTCGGA CCAATTCTTT CAACAACCAC CAAAAGGTGA CTGTGAGGCA GACATTGCCC 
CCAACAGCTA GTGTTGCTGG GAAGCCAGAG TCATCTTTGG GCCCACCTAA CACAATAACT 
GCAGTGACCT CTCCACCAAT TCAGCCACCT GTTAAGAGTA TACGGGTCAT GAGGCCTGAG 
CCGAAGACAG CAGTGGGACC ATGCCATCCA GGGTGGCTGA GTGCACAATC CCCGGAAGCA 
CAGGATTCTC TTGGATACAT CCCAGATGAG AATTTTACCC TGGAACCCAG TCAAGAACAT 
CGATGCCCAC CTCCACCTTA CCCCAAAACA CTGCTTGTGT CTGGATCTGG ACCTGAGCCT 
GCATCCTTAG AGCCTGTAGC CATGAGTGGA CTTCAAGATG TCAGTGCCAC AGGTAGGACT 
ATCCAGAGTT CCTCGGTAGG AAAGCAGGAG GAATCCATCT TCAAAGACAA AGTGAAAATG 
GGCAAAGGAG AAAAGGCTGT GAAGGATAAG AAGCAGATCC AGACATCACC TGTGCCTGTA 
CGAAAAAATG CCCGTGATGA AGAGAAGAGG GAGTCTCGTA TTAAGAGTTA TTCTCCATTT 
GCCTTTAAGT TCTACATGGA GCAACATGTG GAGAATGTGA TGAAGACATA CCAGCAGAAA 
CTTAATCGCA GAATGCAGCT GGAGCAAGAG ATGTCCAAGG CTGGCCTTTC AGAGGCTGAG 
CAGGAACAGA TGAGAAAGAT GCTTTACCAG AAGGAATCCA ATTACAATCG ACTACGACGT 
GCCAAGATGG ACAAATCAAT GTTTGTCAAA ATCAAGACAC TCGGCATTGG GGCCTTTGGT 
GAGGTGTGTC TAACCCGAAA AGTAGACACA GGAGCCTTGT ATGCAATGAA GACTTTGCGA 
AAGAAAGACG TTCTTAACCG AAATCAAGTA GCCCATGTTA AAGCTGAACG GGACATTTTA 
GCAGAGGCTG ACAATGAGTG GGTAGTCAGG TTGTACTATT CATTTCAAGA CCGAGACAAT 
CTGTATTTTG TCATGGATTA CATTCCTGGA GGAGATATGA TGAGCTTGCT GATCCGTATG 
GGAGTTTTTC CAGAGGTCTT AGCCAGGTTT TATGTGGCAG AACTGACACT GGCAATTGAG 
AGTGTGCATA AGATGGGTTT CATCCACAGA GATATCAAGC CAGACAATAT CCTCATTGAC 
CTGGATGGAC ACATCAAACT GACTGATTTT GGTCTATGTA CGGGGTTCCG ATGGACTCAC 
AACTCAAAGT ATTACCAAAA AGGGAACCAT ATCCGACAGG ATAGCATGGA GCCAAGTGAC 
TTTTGGGATG ATGTGTCTAA TTGCCGTTGT GGTGACCGGC TGATGACTTT GGAACAGAGA 
GCAACTCGAC AACACCAGCG CTGCCTGGCG CATTCTTTGG TGGGCACCCC TAACTACATT 
GCCCCTGAGG TGCTTTTGCG CAAAGGATAC ACACAGCTGT GCGACTGGTG GAGTGTGGGA 
GTGATCCTGT TTGAGATGCT GGTTGGCCAA CCACCCTTTC TTGCTCCAAC CCCAACAGAG 
ACGCAGCTTA AGGTGATAAA CTGGGAGAAC ACTTTGCAAG TGCCACCACA AGTAAAACTA 
AGCCCAGAGG CAGTCGATAT TATTGGCCAG CTTTGCTGCT CTGCTGAAGA GCGTCTAGGA 
GGCAATGGGG CTGGAGAAAT AAAGGCTCAT CCTTTTTTTT CCGAGGTGGA TTTCTCCAGC 
AATCTTCGGA CACAACCTGC ACCATATCGA CCAAAAATTG CCCACCCCAT GGATACATCC 
AACTTTGATC CTGTTGAAGA GGAGGGAGGC CCAGGCGCCT GGAGTGATAG TGGGGATAGC 
ACCCGCACTT GGGACACCCT CTGTTCTCCT CACGGCAAAC ACCCTGAACA TGCCTTCTAT 
GAGTTTACTT TCCGAAGATT TTTTGATGAT AATGGTTGTC CATTCCGTTA TCCCAAACCC 
CTAGAGGCGA CACAAAGCCT GTCCAGCAGT GACGGGGCCG GCAGCCTGAA GCCTGAGCTG 
GAGGAGGAAG AGGCCCATGA AGAGGAAGAG CAAGGGGAAG GGTGTGAGCC GGTTTATGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats2 ( XM_021478741.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334416.1 Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X2, mRNA.

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CloneID	ODa15095
Clone ID Related Accession (Same CDS sequence)	XM_005167579.3 , XM_005167579.4 , XM_021478741.1
Accession Version	XM_021478741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase LATS2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCGA AGACGTTCCC TGCCACTCCA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCACT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAATTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAAAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT GCTACCATGG GAAACCCGAT GGGACGCCCT 
GCAAGTATGG GTGCTTACTC CACAAACATG GCTCCTCAAA ATAATACTGG GAATCCAATG 
TACACCCCTG GTCCAGCCCA GCAAAAAGGA TACCCTAGTG GGATTGACCA GGCCATGATG 
GGGTACAGTG TGTCTGGACC ACCCATGCAA ATCCAGTCTC AGCCCTCAGG AGGGCCTGGA 
GCACATTATG ACTACAGGTC GCATATGATG GAGAATTCCA GCTACGGGGT CAAAAGAAGT 
GCTTCCTTTC AGAATAAGAT GCCCCAACTA ACTACAGAAA ATTATGTCAA TATGCCAAGC 
AAAGCAGCAA TGGGCCAAAA TGCTGGTGGC TATGCTCCCA ACTTATATCT GCCACCACAT 
TCACATCCAC GCCAAGCTAG CCCCACCTCA CACCAGGTTC ACATGATGCA GCGCCCCCCA 
GGTGTTGCCC CTGGAGCTAT GGGTGGGGAG TTCTCTGATG TTCCTCAAGG CCTTCTCACC 
CCTTCCAGGG CCAGTTTAAA TCTTGACCTG TATGAGCATC ACTGGCCAGG AGCTCAAGGT 
CCTGAGGGGA CTCCTCCAGC CAGGCAGCCA CAAGGGCCCT TCAGAGGGGA GGTACGCGTC 
CCCAGTCGGA CCAATTCTTT CAACAACCAC CAAAAGGTGA CTGTGAGGCA GACATTGCCC 
CCAACAGCTA GTGTTGCTGG GAAGCCAGAG TCATCTTTGG GCCCACCTAA CACAATAACT 
GCAGTGACCT CTCCACCAAT TCAGCCACCT GTTAAGAGTA TACGGGTCAT GAGGCCTGAG 
CCGAAGACAG CAGTGGGACC ATGCCATCCA GGGTGGCTGA GTGCACAATC CCCGGAAGCA 
CAGGATTCTC TTGGATACAT CCCAGATGAG AATTTTACCC TGGAACCCAG TCAAGAACAT 
CGATGCCCAC CTCCACCTTA CCCCAAAACA CTGCTTGTGT CTGGATCTGG ACCTGAGCCT 
GCATCCTTAG AGCCTGTAGC CATGAGTGGA CTTCAAGATG TCAGTGCCAC AGGTAGGACT 
ATCCAGAGTT CCTCGGTAGG AAAGCAGGAG GAATCCATCT TCAAAGACAA AGTGAAAATG 
GGCAAAGGAG AAAAGGCTGT GAAGGATAAG AAGCAGATCC AGACATCACC TGTGCCTGTA 
CGAAAAAATG CCCGTGATGA AGAGAAGAGG GAGTCTCGTA TTAAGAGTTA TTCTCCATTT 
GCCTTTAAGT TCTACATGGA GCAACATGTG GAGAATGTGA TGAAGACATA CCAGCAGAAA 
CTTAATCGCA GAATGCAGCT GGAGCAAGAG ATGTCCAAGG CTGGCCTTTC AGAGGCTGAG 
CAGGAACAGA TGAGAAAGAT GCTTTACCAG AAGGAATCCA ATTACAATCG ACTACGACGT 
GCCAAGATGG ACAAATCAAT GTTTGTCAAA ATCAAGACAC TCGGCATTGG GGCCTTTGGT 
GAGGTGTGTC TAACCCGAAA AGTAGACACA GGAGCCTTGT ATGCAATGAA GACTTTGCGA 
AAGAAAGACG TTCTTAACCG AAATCAAGTA GCCCATGTTA AAGCTGAACG GGACATTTTA 
GCAGAGGCTG ACAATGAGTG GGTAGTCAGG TTGTACTATT CATTTCAAGA CCGAGACAAT 
CTGTATTTTG TCATGGATTA CATTCCTGGA GGAGATATGA TGAGCTTGCT GATCCGTATG 
GGAGTTTTTC CAGAGGTCTT AGCCAGGTTT TATGTGGCAG AACTGACACT GGCAATTGAG 
AGTGTGCATA AGATGGGTTT CATCCACAGA GATATCAAGC CAGACAATAT CCTCATTGAC 
CTGGATGGAC ACATCAAACT GACTGATTTT GGTCTATGTA CGGGGTTCCG ATGGACTCAC 
AACTCAAAGT ATTACCAAAA AGGGAACCAT ATCCGACAGG ATAGCATGGA GCCAAGTGAC 
TTTTGGGATG ATGTGTCTAA TTGCCGTTGT GGTGACCGGC TGATGACTTT GGAACAGAGA 
GCAACTCGAC AACACCAGCG CTGCCTGGCG CATTCTTTGG TGGGCACCCC TAACTACATT 
GCCCCTGAGG TGCTTTTGCG CAAAGGATAC ACACAGCTGT GCGACTGGTG GAGTGTGGGA 
GTGATCCTGT TTGAGATGCT GGTTGGCCAA CCACCCTTTC TTGCTCCAAC CCCAACAGAG 
ACGCAGCTTA AGGTGATAAA CTGGGAGAAC ACTTTGCAAG TGCCACCACA AGTAAAACTA 
AGCCCAGAGG CAGTCGATAT TATTGGCCAG CTTTGCTGCT CTGCTGAAGA GCGTCTAGGA 
GGCAATGGGG CTGGAGAAAT AAAGGCTCAT CCTTTTTTTT CCGAGGTGGA TTTCTCCAGC 
AATCTTCGGA CACAACCTGC ACCATATCGA CCAAAAATTG CCCACCCCAT GGATACATCC 
AACTTTGATC CTGTTGAAGA GGAGGGAGGC CCAGGCGCCT GGAGTGATAG TGGGGATAGC 
ACCCGCACTT GGGACACCCT CTGTTCTCCT CACGGCAAAC ACCCTGAACA TGCCTTCTAT 
GAGTTTACTT TCCGAAGATT TTTTGATGAT AATGGTTGTC CATTCCGTTA TCCCAAACCC 
CTAGAGGCGA CACAAAGCCT GTCCAGCAGT GACGGGGCCG GCAGCCTGAA GCCTGAGCTG 
GAGGAGGAAG AGGCCCATGA AGAGGAAGAG CAAGGGGAAG GGTGTGAGCC GGTTTATGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334416.1
CDS	617..4039
Translation	MRPKTFPATPYVGNTRQRLQDIKEGLKQPAKLVSQALHGGSSRGEGGRGGDSKGKDQAARQQQLRPPQKFNNYQSALREIRKSLRPYANDSGPGSSSGHPGEVSRQMLQDLVNAGCDQEMAVRALKQTGSRNIEAALEYISKMSYLDPHTEQIVRVIKQTSPGKSGMPNLMDHRTAMEGSGDGALPPYHQMGAPLYEGASYGAEGEMARAYMGGPPVMNYMPPPANPSQGATMGNPMGRPASMGAYSTNMAPQNNTGNPMYTPGPAQQKGYPSGIDQAMMGYSVSGPPMQIQSQPSGGPGAHYDYRSHMMENSSYGVKRSASFQNKMPQLTTENYVNMPSKAAMGQNAGGYAPNLYLPPHSHPRQASPTSHQVHMMQRPPGVAPGAMGGEFSDVPQGLLTPSRASLNLDLYEHHWPGAQGPEGTPPARQPQGPFRGEVRVPSRTNSFNNHQKVTVRQTLPPTASVAGKPESSLGPPNTITAVTSPPIQPPVKSIRVMRPEPKTAVGPCHPGWLSAQSPEAQDSLGYIPDENFTLEPSQEHRCPPPPYPKTLLVSGSGPEPASLEPVAMSGLQDVSATGRTIQSSSVGKQEESIFKDKVKMGKGEKAVKDKKQIQTSPVPVRKNARDEEKRESRIKSYSPFAFKFYMEQHVENVMKTYQQKLNRRMQLEQEMSKAGLSEAEQEQMRKMLYQKESNYNRLRRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTGALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRDNLYFVMDYIPGGDMMSLLIRMGVFPEVLARFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGNHIRQDSMEPSDFWDDVSNCRCGDRLMTLEQRATRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLQVPPQVKLSPEAVDIIGQLCCSAEERLGGNGAGEIKAHPFFSEVDFSSNLRTQPAPYRPKIAHPMDTSNFDPVEEEGGPGAWSDSGDSTRTWDTLCSPHGKHPEHAFYEFTFRRFFDDNGCPFRYPKPLEATQSLSSSDGAGSLKPELEEEEAHEEEEQGEGCEPVYV

Target ORF information:

RefSeq Version	XM_021478741.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio large tumor suppressor kinase 2 (lats2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478741.1

ORF Insert Sequence:

ATGAGGCCGA AGACGTTCCC TGCCACTCCA TATGTGGGCA ACACACGCCA GCGCCTGCAG 
GACATCAAGG AGGGTCTGAA ACAGCCAGCG AAGCTGGTGA GTCAGGCACT TCATGGAGGC 
AGCTCTCGAG GAGAGGGTGG TCGAGGTGGA GACTCAAAGG GCAAAGACCA GGCTGCCAGA 
CAACAGCAGC TCCGGCCGCC CCAGAAGTTT AACAACTACC AGAGTGCCCT CCGAGAGATC 
CGCAAGTCTC TGAGGCCCTA TGCCAATGAT TCAGGTCCAG GATCCAGCTC CGGGCATCCC 
GGTGAGGTCA GCAGACAGAT GCTACAGGAT CTGGTCAATG CCGGTTGCGA TCAGGAAATG 
GCAGTGCGTG CCCTGAAACA GACTGGTAGC CGTAACATCG AGGCAGCATT GGAATACATT 
AGCAAAATGA GTTACCTTGA TCCTCACACT GAACAGATTG TACGTGTCAT CAAACAGACT 
TCCCCAGGAA AAAGTGGCAT GCCAAATTTG ATGGACCACA GGACAGCAAT GGAAGGCAGT 
GGGGATGGTG CACTTCCCCC TTATCATCAA ATGGGAGCAC CATTGTATGA GGGAGCTAGT 
TATGGGGCTG AGGGTGAAAT GGCTCGGGCA TACATGGGTG GACCACCTGT CATGAACTAC 
ATGCCACCGC CTGCCAACCC TTCACAGGGT GCTACCATGG GAAACCCGAT GGGACGCCCT 
GCAAGTATGG GTGCTTACTC CACAAACATG GCTCCTCAAA ATAATACTGG GAATCCAATG 
TACACCCCTG GTCCAGCCCA GCAAAAAGGA TACCCTAGTG GGATTGACCA GGCCATGATG 
GGGTACAGTG TGTCTGGACC ACCCATGCAA ATCCAGTCTC AGCCCTCAGG AGGGCCTGGA 
GCACATTATG ACTACAGGTC GCATATGATG GAGAATTCCA GCTACGGGGT CAAAAGAAGT 
GCTTCCTTTC AGAATAAGAT GCCCCAACTA ACTACAGAAA ATTATGTCAA TATGCCAAGC 
AAAGCAGCAA TGGGCCAAAA TGCTGGTGGC TATGCTCCCA ACTTATATCT GCCACCACAT 
TCACATCCAC GCCAAGCTAG CCCCACCTCA CACCAGGTTC ACATGATGCA GCGCCCCCCA 
GGTGTTGCCC CTGGAGCTAT GGGTGGGGAG TTCTCTGATG TTCCTCAAGG CCTTCTCACC 
CCTTCCAGGG CCAGTTTAAA TCTTGACCTG TATGAGCATC ACTGGCCAGG AGCTCAAGGT 
CCTGAGGGGA CTCCTCCAGC CAGGCAGCCA CAAGGGCCCT TCAGAGGGGA GGTACGCGTC 
CCCAGTCGGA CCAATTCTTT CAACAACCAC CAAAAGGTGA CTGTGAGGCA GACATTGCCC 
CCAACAGCTA GTGTTGCTGG GAAGCCAGAG TCATCTTTGG GCCCACCTAA CACAATAACT 
GCAGTGACCT CTCCACCAAT TCAGCCACCT GTTAAGAGTA TACGGGTCAT GAGGCCTGAG 
CCGAAGACAG CAGTGGGACC ATGCCATCCA GGGTGGCTGA GTGCACAATC CCCGGAAGCA 
CAGGATTCTC TTGGATACAT CCCAGATGAG AATTTTACCC TGGAACCCAG TCAAGAACAT 
CGATGCCCAC CTCCACCTTA CCCCAAAACA CTGCTTGTGT CTGGATCTGG ACCTGAGCCT 
GCATCCTTAG AGCCTGTAGC CATGAGTGGA CTTCAAGATG TCAGTGCCAC AGGTAGGACT 
ATCCAGAGTT CCTCGGTAGG AAAGCAGGAG GAATCCATCT TCAAAGACAA AGTGAAAATG 
GGCAAAGGAG AAAAGGCTGT GAAGGATAAG AAGCAGATCC AGACATCACC TGTGCCTGTA 
CGAAAAAATG CCCGTGATGA AGAGAAGAGG GAGTCTCGTA TTAAGAGTTA TTCTCCATTT 
GCCTTTAAGT TCTACATGGA GCAACATGTG GAGAATGTGA TGAAGACATA CCAGCAGAAA 
CTTAATCGCA GAATGCAGCT GGAGCAAGAG ATGTCCAAGG CTGGCCTTTC AGAGGCTGAG 
CAGGAACAGA TGAGAAAGAT GCTTTACCAG AAGGAATCCA ATTACAATCG ACTACGACGT 
GCCAAGATGG ACAAATCAAT GTTTGTCAAA ATCAAGACAC TCGGCATTGG GGCCTTTGGT 
GAGGTGTGTC TAACCCGAAA AGTAGACACA GGAGCCTTGT ATGCAATGAA GACTTTGCGA 
AAGAAAGACG TTCTTAACCG AAATCAAGTA GCCCATGTTA AAGCTGAACG GGACATTTTA 
GCAGAGGCTG ACAATGAGTG GGTAGTCAGG TTGTACTATT CATTTCAAGA CCGAGACAAT 
CTGTATTTTG TCATGGATTA CATTCCTGGA GGAGATATGA TGAGCTTGCT GATCCGTATG 
GGAGTTTTTC CAGAGGTCTT AGCCAGGTTT TATGTGGCAG AACTGACACT GGCAATTGAG 
AGTGTGCATA AGATGGGTTT CATCCACAGA GATATCAAGC CAGACAATAT CCTCATTGAC 
CTGGATGGAC ACATCAAACT GACTGATTTT GGTCTATGTA CGGGGTTCCG ATGGACTCAC 
AACTCAAAGT ATTACCAAAA AGGGAACCAT ATCCGACAGG ATAGCATGGA GCCAAGTGAC 
TTTTGGGATG ATGTGTCTAA TTGCCGTTGT GGTGACCGGC TGATGACTTT GGAACAGAGA 
GCAACTCGAC AACACCAGCG CTGCCTGGCG CATTCTTTGG TGGGCACCCC TAACTACATT 
GCCCCTGAGG TGCTTTTGCG CAAAGGATAC ACACAGCTGT GCGACTGGTG GAGTGTGGGA 
GTGATCCTGT TTGAGATGCT GGTTGGCCAA CCACCCTTTC TTGCTCCAAC CCCAACAGAG 
ACGCAGCTTA AGGTGATAAA CTGGGAGAAC ACTTTGCAAG TGCCACCACA AGTAAAACTA 
AGCCCAGAGG CAGTCGATAT TATTGGCCAG CTTTGCTGCT CTGCTGAAGA GCGTCTAGGA 
GGCAATGGGG CTGGAGAAAT AAAGGCTCAT CCTTTTTTTT CCGAGGTGGA TTTCTCCAGC 
AATCTTCGGA CACAACCTGC ACCATATCGA CCAAAAATTG CCCACCCCAT GGATACATCC 
AACTTTGATC CTGTTGAAGA GGAGGGAGGC CCAGGCGCCT GGAGTGATAG TGGGGATAGC 
ACCCGCACTT GGGACACCCT CTGTTCTCCT CACGGCAAAC ACCCTGAACA TGCCTTCTAT 
GAGTTTACTT TCCGAAGATT TTTTGATGAT AATGGTTGTC CATTCCGTTA TCCCAAACCC 
CTAGAGGCGA CACAAAGCCT GTCCAGCAGT GACGGGGCCG GCAGCCTGAA GCCTGAGCTG 
GAGGAGGAAG AGGCCCATGA AGAGGAAGAG CAAGGGGAAG GGTGTGAGCC GGTTTATGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth.
Disease models & mechanisms10(1)15-28(2017 01)
Mayrhofer M,Gourain V,Reischl M,Affaticati P,Jenett A,Joly JS,Benelli M,Demichelis F,Poliani PL,Sieger D,Mione M

Yap and Taz regulate retinal pigment epithelial cell fate.
Development (Cambridge, England)142(17)3021-32(2015 Sep)
Miesfeld JB,Gestri G,Clark BS,Flinn MA,Poole RJ,Bader JR,Besharse JC,Wilson SW,Link BA

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lats2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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