tnfsf12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnfsf12
Entrez Gene ID	567542
Full Name	TNF superfamily member 12
Synonyms	zgc:153941
General protein information	Preferred Names TNF superfamily member 12 Names tumor necrosis factor ligand superfamily member 12 TWEAK tumor necrosis factor (ligand) superfamily, member 12 tumor necrosis factor superfamily member 12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076607.1	NP_001070075.1	tumor necrosis factor ligand superfamily member 12

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TNFSF12	NP_001192058.1
Rattus norvegicus (Norway rat)	Tnfsf12	NP_001001513.2
Danio rerio (zebrafish)	tnfsf12	NP_001070075.1
Canis lupus familiaris (dog)	TNFSF12	NP_001192097.1
Mus musculus (house mouse)	Tnfsf12	NP_035744.1
Homo sapiens (human)	TNFSF12	NP_003800.1
Bos taurus (cattle)	TNFSF12	NP_001192072.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Biswas G, Kinoshita S, Kono T, Hikima J, Sakai M
Marine genomics2225-36(2015 Aug)

Molecular cloning, phylogenetic analysis, tissue distribution, and expression of the cytokine TWEAK in zebrafish (Danio rerio).
Du M, Liang Z, Xu X, Li J, Ai H, Zhang J, Zhang S
Biochemical genetics50(3-4)318-24(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

zTWEAK gene was constitutively expressed in all detected tissues, with the highest mRNA levels observed in lymphoid tissue of the spleen.
Title: Molecular cloning, phylogenetic analysis, tissue distribution, and expression of the cytokine TWEAK in zebrafish (Danio rerio).

The following tnfsf12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnfsf12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34843	NM_001076607.1 Latest version!	Danio rerio TNF superfamily member 12 (tnfsf12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnfsf12 ( NM_001076607.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070075.1 Danio rerio TNF superfamily member 12 (tnfsf12), mRNA.

Price & Availability↑

CloneID	ODa34843
Clone ID Related Accession (Same CDS sequence)	NM_001076607.1
Accession Version	NM_001076607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-02
Organism	Danio rerio(zebrafish)
Product	tumor necrosis factor ligand superfamily member 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124495.1. On Sep 29, 2007 this sequence version replaced XM_690838.2. ##Evidence-Data-START## Transcript exon combination :: BC124495.1, BC154255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCGAA TTTTCCAGAG GAGACGGATC CGCACGCTGC GCTGGGTCTG GTCGCTGATG 
GCCGTGCTCG CGCTGTCTCT GGCCGTGTGC AGTGCCATAT TTACAGTGTG GACGCTGCGA 
CAAACGCGGG ACCTGTCGAG GTCTTTCCAA ACTCTGCAGG AGCGCTTGGA GCAGGTGAAT 
ATGCAGCGTA AGGCCATCTT CCAACTGATC CTGGAAAAAC GCGAACTGTT GGAGAGCCAG 
AGGTTCCGCA GAGATGTGGG AGGGAAGAAA GGAAACGGAC GAAAAGTGGC CTCTCATTTT 
GAGATAACCA GTGATTCCTT CCAAAAAGTT GGAAACGAAG GAGTCATTAA AGGATGGACT 
GAGGAGCAGC TGAATATGAG CAGGGCAGTG AACTACAATT CAGAGACCGG CACCTTCAGA 
GTGGAGCGTA GCGGCGTCTA CTTCGTGTAT TGTCAAGTAC ACTTCAACGA GAACCAGAGT 
CAGTATGTGA AGCTCGAAGT GTCCATTCCT AAAGGCCCTT TGCTCCAGTG CATTGAAGGG 
TACGGAACCA CTCCAGCCTC TGGCTCTCAC AGGTTTCACT TCCTGAAGCC ATGCCAGGTC 
TCCGGCCTCC TTCGCCTTAA CAAGGGGACC GAGCTGAAGG CTGTCACCGG AGCTTCCTTC 
AGTCTGCAGA CGTCAGGCAA GCACTATTTC GGCCTCTTTA AAGTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070075.1
CDS	330..1040
Translation	MQRIFQRRRIRTLRWVWSLMAVLALSLAVCSAIFTVWTLRQTRDLSRSFQTLQERLEQVNMQRKAIFQLILEKRELLESQRFRRDVGGKKGNGRKVASHFEITSDSFQKVGNEGVIKGWTEEQLNMSRAVNYNSETGTFRVERSGVYFVYCQVHFNENQSQYVKLEVSIPKGPLLQCIEGYGTTPASGSHRFHFLKPCQVSGLLRLNKGTELKAVTGASFSLQTSGKHYFGLFKVN

Target ORF information:

RefSeq Version	NM_001076607.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TNF superfamily member 12 (tnfsf12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076607.1

ORF Insert Sequence:

ATGCAGCGAA TTTTCCAGAG GAGACGGATC CGCACGCTGC GCTGGGTCTG GTCGCTGATG 
GCCGTGCTCG CGCTGTCTCT GGCCGTGTGC AGTGCCATAT TTACAGTGTG GACGCTGCGA 
CAAACGCGGG ACCTGTCGAG GTCTTTCCAA ACTCTGCAGG AGCGCTTGGA GCAGGTGAAT 
ATGCAGCGTA AGGCCATCTT CCAACTGATC CTGGAAAAAC GCGAACTGTT GGAGAGCCAG 
AGGTTCCGCA GAGATGTGGG AGGGAAGAAA GGAAACGGAC GAAAAGTGGC CTCTCATTTT 
GAGATAACCA GTGATTCCTT CCAAAAAGTT GGAAACGAAG GAGTCATTAA AGGATGGACT 
GAGGAGCAGC TGAATATGAG CAGGGCAGTG AACTACAATT CAGAGACCGG CACCTTCAGA 
GTGGAGCGTA GCGGCGTCTA CTTCGTGTAT TGTCAAGTAC ACTTCAACGA GAACCAGAGT 
CAGTATGTGA AGCTCGAAGT GTCCATTCCT AAAGGCCCTT TGCTCCAGTG CATTGAAGGG 
TACGGAACCA CTCCAGCCTC TGGCTCTCAC AGGTTTCACT TCCTGAAGCC ATGCCAGGTC 
TCCGGCCTCC TTCGCCTTAA CAAGGGGACC GAGCTGAAGG CTGTCACCGG AGCTTCCTTC 
AGTCTGCAGA CGTCAGGCAA GCACTATTTC GGCCTCTTTA AAGTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Marine genomics2225-36(2015 Aug)
Biswas G,Kinoshita S,Kono T,Hikima J,Sakai M

Molecular cloning, phylogenetic analysis, tissue distribution, and expression of the cytokine TWEAK in zebrafish (Danio rerio).
Biochemical genetics50(3-4)318-24(2012 Apr)
Du M,Liang Z,Xu X,Li J,Ai H,Zhang J,Zhang S