tsc2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tsc2
Entrez Gene ID	567524
Full Name	TSC complex subunit 2
Synonyms	fc16b02,tuberin,wu:fc16b02
General protein information	Preferred Names TSC complex subunit 2 Names tuberin tuberous sclerosis 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009294973.2	XP_009293248.2	tuberin isoform X1
XM_017355410.1	XP_017210899.1	tuberin isoform X2
NM_001328401.1	NP_001315330.1	tuberin
XM_021475593.1	XP_021331268.1	tuberin isoform X1
XM_021475598.1	XP_021331273.1	tuberin isoform X3
XM_021475594.1	XP_021331269.1	tuberin isoform X2
XM_021475603.1	XP_021331278.1	tuberin isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSC2	XP_523477.3
Mus musculus (house mouse)	Tsc2	NP_001034452.1
Rattus norvegicus (Norway rat)	Tsc2	NP_036812.2
Gallus gallus (chicken)	TSC2	XP_003642168.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011123	XP_309523.4
Canis lupus familiaris (dog)	TSC2	XP_852047.2
Bos taurus (cattle)	TSC2	XP_003587856.2
Drosophila melanogaster (fruit fly)	gig	NP_524177.1
Homo sapiens (human)	TSC2	NP_000539.2
	TSC2	XP_001083256.1
Danio rerio (zebrafish)	tsc2	XP_695912.6
Xenopus tropicalis (tropical clawed frog)	tsc2	NP_001123795.1

Gene Ontology
Bibliography

Related articles in PubMed

Lithium promotes the production of reactive oxygen species via GSK-3?/TSC2/TOR signaling in the gill of zebrafish (Danio rerio).
Liu D, Gao L, Zhang Z, Tao S, Pang Q, Li A, Deng H, Yu H
Chemosphere195854-863(2018 Mar)

mTOR-related neuropathology in mutant tsc2 zebrafish: Phenotypic, transcriptomic and pharmacological analysis.
Scheldeman C, Mills JD, Siekierska A, Serra I, Copmans D, Iyer AM, Whalley BJ, Maes J, Jansen AC, Lagae L, Aronica E, de Witte PAM
Neurobiology of disease108225-237(2017 Dec)

Transcriptomic profiling of mTOR and ryanodine receptor signaling molecules in developing zebrafish in the absence and presence of PCB 95.
Frank DF, Miller GW, Connon RE, Geist J, Lein PJ
PeerJ5e4106(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first report using an animal model to show interactions between tsc2, mTORC1 and p53 during tumorigenesis.
Title: Heterozygous inactivation of tsc2 enhances tumorigenesis in p53 mutant zebrafish.

These results demonstrate a highly conserved role of tsc2 in zebrafish and establish a new animal model for studies of Tuberous sclerosis complex.
Title: Zebrafish model of tuberous sclerosis complex reveals cell-autonomous and non-cell-autonomous functions of mutant tuberin.

The following tsc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tsc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa147276	XM_021475593.1 Latest version!	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa147277	XM_021475594.1 Latest version!	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa147278	XM_021475598.1 Latest version!	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa60579	NM_001328401.1 Latest version!	Danio rerio TSC complex subunit 2 (tsc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa147279	XM_021475603.1 Latest version!	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa53725	XM_009294973.2 Latest version!	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa53726	XM_017355410.1 Latest version!	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tsc2 ( XM_021475593.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331268.1 Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147276
Clone ID Related Accession (Same CDS sequence)	XM_021475593.1
Accession Version	XM_021475593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGCA CCATCCAGTA TAAAGTGCTG ATCCTGACGT CTCCATGTAA CATTGATAAT 
CTGTGCGCAA CGCTCTGCTC GATGGTGACG GACCGCATTA TTTTGGAGCG TCTGAAGAAA 
ACCCCTGATG GATTTTCCCT CACTGACGTT CAGCTCGCTG TGGTTCCTGT TCTCACGGCC 
ATCACGTCCT ACCACAGCTA TCTGGATCAG CTCCGACAGA GGGACCTGGT GCAGTGTCTG 
GAGAAGGGTC TGATCTACCG CTGTGCCAAG CAGTGTGTGG TGGCCCTGAC AATGTGCACA 
GTGGAGATGC CCGACATCAT GATCAAACTA CTGCCCGCCC TTATCGTCAA GCTCACGCAC 
ATCTCGGCAA CCGTTGCCAT GGCCTCACCC ATGCTGGAGT TCCTGTCCAC ATTAGTGCGG 
CTCCCACATC TGTATGCTAA CTTCGTAGCT GAGCAGTATG TTAGCGTGTT TGCCATATCG 
CTGCCGTACA CCAATCCTTC AAAGTTTAAT CAGTACATAG TGTCTCTGGC TCATCATGTG 
ATCGCCATGT GGTTCATCCG CTGCCGACTG CCTTTCAGGA AAGATTTTGT TCAGTACATC 
ACCAAGGGTC TTCGTTCTAA CGCTCTCCTG CCGTTTGACG ACACACACGA GCAGAGCAGT 
TTCAGAGCAC GCAGCACCAG CCTGAACGAG AGGCCCAAGA GTTTGCGGAC GACCAAAGTG 
GCGAAGCAAG GCCCCAGCGC AAACTCTCCC GTTAAAGACC TGAAAGACCT TTCGGCCATG 
GACGCCTTCC GCTCCCGCAG CATCAGTGTG TCCGAGCATG CTGTGCGCAG GATGCAGACG 
TCCAGCACCA CCTGCAGTCT GGGCTCAGCT GATGAGAACG CTGTGATGCA GGCGGACGAC 
GCTCTGAAGA CAGTCCATCT GGAGCTCACA GAGACCTGCC TGGACATGAT GGCCAGATAC 
GTCTTCTCCA ACTTCTCTGC TCTGCCCAAG AGGTCTCCCA TCGCTGATTT CCTGTTATCT 
GGTGGTCCTA GTATGACGTG GCTGGTTGGG AATAAGTTAG TGACCATAAC CACCAGCGGC 
GGCTCGCGAA CACAAGCTCT TCTGGGTCTG GACATGACAG AGAGACATGG AGGAGGAGAA 
ATGACCAGGT CTGACCCGTC TCTGCACACC CGACAGACCA AAGAAGCTCC CGCTAAACTA 
GAGTCTCAGT CCAGCCAACA GATCAACACC AACACACGCA CACGAGTCCG CTCTATATCA 
GGTGGTCATG CTCTGCGGGC CGGGCCAGCT CAGAGTCTTA GTCCTCTGGT GGCGTCTCCT 
GAAGGCGAGT ACAGCGGCCC GCTGCCTCCT CCCGGACCCC CGCTGGACGT CCTGAGCTCT 
CAGAGGGGGA TGGAGGAGCA CCCGCCACCC TCCGCCTGCA ACCCATCACC ACCGCTCAAG 
CACAAGCACA GCCTGGCAGA GTTTGTGCCC ATGCTGACGC AGGGCTGGGC CGAGATCTTC 
ATCCGCAGGC CCTCAGGGAA CACGAGCTGG CTGATGTGTC TGGAGAACCC GCCAAGCCCG 
TTCTCCTCTG AGCTGGGTAA TCTGCCTCTG CAGGAGCTCT CCAGCGTCCT CATGGCCATG 
GACGGAGTGA AGGAGCCACC GGCCGAGCGT CCCACAGACG TGTCCCAGCC ACAGGGCAAA 
CCGGCTCCCA TCCAGCGATC CAACACTGTG GCCTCTCTCA GCTCTCTCTG CTCCTCTGAA 
TCATCCGGCA GGTTTCACAG GAGTATATCC TGGGCAGATT CAGTAGTGAT GGAGGAAAGT 
GGGCGGAGTA TGGCGTCTGT TTCACCTGCT GAGTCTAAAG AGGTGGAGGA GTTTGAGGCC 
GTTCCTTCTG AACCCATCTT CATGAACACC AGCACGACTG CACCCGGCAT GATGAGCAGA 
TCCTCCTCCA CATCCAGTCA GGATGATGAT AAGTCCACCC TGGAGGAGGT CAGTGAAGGA 
GGAATCCCCA TCGACCAGCC TCCGCTCAGC CTCTGCACCC CTGGAGCCCA GGACCCCGAG 
CTTTCCTTCA CCCAAAGCAG CAGCAGCTCC AGCACCCTTA ATAAATCCAG CTCCTCTCCT 
GAGCTCCAGA CCCTTCCAGA GGCCTTCAGC AAAGCCTCGT CCCAGAGCGT GGATCCAGCT 
CCCACCAGCA AAATCCCTAC AGTCCAGGCC TGTGTGGAGG CTCCAGGAGA GAGCGCTGGA 
TCCACCGCTG GGACATCTTG CAGCAGCTCT AGCGTGTCCG TGTCCTCCTC CTCCACCTCC 
AGAATGAGGC TGGAGTTCCC GCCAGTGCAG ACAGGACCCC TGTCTCCTAC TGGACACCGG 
CCACGGGGAC ACACCATATC AGTGTCTGCT CCATCGTCCA GGAGAGACCG CAAAACACTG 
GAGCGGGACG CCAGAGGAGG AGCGACCAAT GTGGAGAAGA GCTCCGGACT CAGCCCCAGT 
TTTGTCTTCC TGCAGCTTTA TCACTCGCCG TTCTTCGGCA ATGAGGCCAA CAAACCCCTG 
CTGCTTCCTA AATCACAGCT GATTGACCGT GCAGTGAAGG TTTTGGATCA GATGCCTCCG 
TACGACACTC ATAAGATCGG TGTGGTGTTT GTGGGAGCCG GACAGGCCAA TAATGAGGTG 
TCGATCCTGT CTAATGAGTA CGGCTCGAAG CGCTATGCTC AGTTCCTGAC GGGTCTGGGG 
AAACTCATCC ACTTGAAAGA CTGCGATCCC GATCAGATCT TCCTTGGTGG ACTGGACCAG 
TACGGAGACG ATGGAGAGTT CACATACTGC TGGCATGATG ACATTATGCA GGCTATTTTC 
CACATCGCCA CGCTGATGCC GAACCGTGAG AGCGACCGCG GCTGCTGCAA CAAGAAGCGG 
CACATCGGCA ACGACTTCGT GGTGGTGGTT TATAACGACT CGGGGGAGGA CTACAAACTG 
GGCACCATCA AGGGTCAGTT CAACTTTGTG GAAGTGCTCA TAAAGCCGCT GGACTACGAG 
AGTAACCTGG TGACACTGCA GTGCCGGAAA GATCTGGAGG GTTTGGTGGA CACCAGTGTA 
GCGAAGATCG TCTCTGACCG GAACCTTCCC CTGCTAGTCA GACAGATGGC GCTTCATGCT 
AACATGGCGT CGCTGGTCCA CCAGTGCAGG GCGAACCCAT CAGACGCATA CGCTTCAAAA 
TGGCTGGCCA GACTGAGACA CATCAAGAGG ATCAGGACGC GGGCTCAGGA GGAGATTCAG 
TCCCGCCCCT CTCACGGCAT CTCTCTGACT CAAGGCCACG CCTCCATGCA GAATAAACCC 
GCCCACCAGC CGCCGGGAGC AGCACAGAAT CCAGACGCCG GCCAGAGGAA GAGGCTGGTG 
TCCACCGTCG ACGACTTCAC TGACTTTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331268.1
CDS	164..3556
Translation	MSCTIQYKVLILTSPCNIDNLCATLCSMVTDRIILERLKKTPDGFSLTDVQLAVVPVLTAITSYHSYLDQLRQRDLVQCLEKGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDTHEQSSFRARSTSLNERPKSLRTTKVAKQGPSANSPVKDLKDLSAMDAFRSRSISVSEHAVRRMQTSSTTCSLGSADENAVMQADDALKTVHLELTETCLDMMARYVFSNFSALPKRSPIADFLLSGGPSMTWLVGNKLVTITTSGGSRTQALLGLDMTERHGGGEMTRSDPSLHTRQTKEAPAKLESQSSQQINTNTRTRVRSISGGHALRAGPAQSLSPLVASPEGEYSGPLPPPGPPLDVLSSQRGMEEHPPPSACNPSPPLKHKHSLAEFVPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNLPLQELSSVLMAMDGVKEPPAERPTDVSQPQGKPAPIQRSNTVASLSSLCSSESSGRFHRSISWADSVVMEESGRSMASVSPAESKEVEEFEAVPSEPIFMNTSTTAPGMMSRSSSTSSQDDDKSTLEEVSEGGIPIDQPPLSLCTPGAQDPELSFTQSSSSSSTLNKSSSSPELQTLPEAFSKASSQSVDPAPTSKIPTVQACVEAPGESAGSTAGTSCSSSSVSVSSSSTSRMRLEFPPVQTGPLSPTGHRPRGHTISVSAPSSRRDRKTLERDARGGATNVEKSSGLSPSFVFLQLYHSPFFGNEANKPLLLPKSQLIDRAVKVLDQMPPYDTHKIGVVFVGAGQANNEVSILSNEYGSKRYAQFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDRGCCNKKRHIGNDFVVVVYNDSGEDYKLGTIKGQFNFVEVLIKPLDYESNLVTLQCRKDLEGLVDTSVAKIVSDRNLPLLVRQMALHANMASLVHQCRANPSDAYASKWLARLRHIKRIRTRAQEEIQSRPSHGISLTQGHASMQNKPAHQPPGAAQNPDAGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_021475593.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475593.1

ORF Insert Sequence:

ATGTCCTGCA CCATCCAGTA TAAAGTGCTG ATCCTGACGT CTCCATGTAA CATTGATAAT 
CTGTGCGCAA CGCTCTGCTC GATGGTGACG GACCGCATTA TTTTGGAGCG TCTGAAGAAA 
ACCCCTGATG GATTTTCCCT CACTGACGTT CAGCTCGCTG TGGTTCCTGT TCTCACGGCC 
ATCACGTCCT ACCACAGCTA TCTGGATCAG CTCCGACAGA GGGACCTGGT GCAGTGTCTG 
GAGAAGGGTC TGATCTACCG CTGTGCCAAG CAGTGTGTGG TGGCCCTGAC AATGTGCACA 
GTGGAGATGC CCGACATCAT GATCAAACTA CTGCCCGCCC TTATCGTCAA GCTCACGCAC 
ATCTCGGCAA CCGTTGCCAT GGCCTCACCC ATGCTGGAGT TCCTGTCCAC ATTAGTGCGG 
CTCCCACATC TGTATGCTAA CTTCGTAGCT GAGCAGTATG TTAGCGTGTT TGCCATATCG 
CTGCCGTACA CCAATCCTTC AAAGTTTAAT CAGTACATAG TGTCTCTGGC TCATCATGTG 
ATCGCCATGT GGTTCATCCG CTGCCGACTG CCTTTCAGGA AAGATTTTGT TCAGTACATC 
ACCAAGGGTC TTCGTTCTAA CGCTCTCCTG CCGTTTGACG ACACACACGA GCAGAGCAGT 
TTCAGAGCAC GCAGCACCAG CCTGAACGAG AGGCCCAAGA GTTTGCGGAC GACCAAAGTG 
GCGAAGCAAG GCCCCAGCGC AAACTCTCCC GTTAAAGACC TGAAAGACCT TTCGGCCATG 
GACGCCTTCC GCTCCCGCAG CATCAGTGTG TCCGAGCATG CTGTGCGCAG GATGCAGACG 
TCCAGCACCA CCTGCAGTCT GGGCTCAGCT GATGAGAACG CTGTGATGCA GGCGGACGAC 
GCTCTGAAGA CAGTCCATCT GGAGCTCACA GAGACCTGCC TGGACATGAT GGCCAGATAC 
GTCTTCTCCA ACTTCTCTGC TCTGCCCAAG AGGTCTCCCA TCGCTGATTT CCTGTTATCT 
GGTGGTCCTA GTATGACGTG GCTGGTTGGG AATAAGTTAG TGACCATAAC CACCAGCGGC 
GGCTCGCGAA CACAAGCTCT TCTGGGTCTG GACATGACAG AGAGACATGG AGGAGGAGAA 
ATGACCAGGT CTGACCCGTC TCTGCACACC CGACAGACCA AAGAAGCTCC CGCTAAACTA 
GAGTCTCAGT CCAGCCAACA GATCAACACC AACACACGCA CACGAGTCCG CTCTATATCA 
GGTGGTCATG CTCTGCGGGC CGGGCCAGCT CAGAGTCTTA GTCCTCTGGT GGCGTCTCCT 
GAAGGCGAGT ACAGCGGCCC GCTGCCTCCT CCCGGACCCC CGCTGGACGT CCTGAGCTCT 
CAGAGGGGGA TGGAGGAGCA CCCGCCACCC TCCGCCTGCA ACCCATCACC ACCGCTCAAG 
CACAAGCACA GCCTGGCAGA GTTTGTGCCC ATGCTGACGC AGGGCTGGGC CGAGATCTTC 
ATCCGCAGGC CCTCAGGGAA CACGAGCTGG CTGATGTGTC TGGAGAACCC GCCAAGCCCG 
TTCTCCTCTG AGCTGGGTAA TCTGCCTCTG CAGGAGCTCT CCAGCGTCCT CATGGCCATG 
GACGGAGTGA AGGAGCCACC GGCCGAGCGT CCCACAGACG TGTCCCAGCC ACAGGGCAAA 
CCGGCTCCCA TCCAGCGATC CAACACTGTG GCCTCTCTCA GCTCTCTCTG CTCCTCTGAA 
TCATCCGGCA GGTTTCACAG GAGTATATCC TGGGCAGATT CAGTAGTGAT GGAGGAAAGT 
GGGCGGAGTA TGGCGTCTGT TTCACCTGCT GAGTCTAAAG AGGTGGAGGA GTTTGAGGCC 
GTTCCTTCTG AACCCATCTT CATGAACACC AGCACGACTG CACCCGGCAT GATGAGCAGA 
TCCTCCTCCA CATCCAGTCA GGATGATGAT AAGTCCACCC TGGAGGAGGT CAGTGAAGGA 
GGAATCCCCA TCGACCAGCC TCCGCTCAGC CTCTGCACCC CTGGAGCCCA GGACCCCGAG 
CTTTCCTTCA CCCAAAGCAG CAGCAGCTCC AGCACCCTTA ATAAATCCAG CTCCTCTCCT 
GAGCTCCAGA CCCTTCCAGA GGCCTTCAGC AAAGCCTCGT CCCAGAGCGT GGATCCAGCT 
CCCACCAGCA AAATCCCTAC AGTCCAGGCC TGTGTGGAGG CTCCAGGAGA GAGCGCTGGA 
TCCACCGCTG GGACATCTTG CAGCAGCTCT AGCGTGTCCG TGTCCTCCTC CTCCACCTCC 
AGAATGAGGC TGGAGTTCCC GCCAGTGCAG ACAGGACCCC TGTCTCCTAC TGGACACCGG 
CCACGGGGAC ACACCATATC AGTGTCTGCT CCATCGTCCA GGAGAGACCG CAAAACACTG 
GAGCGGGACG CCAGAGGAGG AGCGACCAAT GTGGAGAAGA GCTCCGGACT CAGCCCCAGT 
TTTGTCTTCC TGCAGCTTTA TCACTCGCCG TTCTTCGGCA ATGAGGCCAA CAAACCCCTG 
CTGCTTCCTA AATCACAGCT GATTGACCGT GCAGTGAAGG TTTTGGATCA GATGCCTCCG 
TACGACACTC ATAAGATCGG TGTGGTGTTT GTGGGAGCCG GACAGGCCAA TAATGAGGTG 
TCGATCCTGT CTAATGAGTA CGGCTCGAAG CGCTATGCTC AGTTCCTGAC GGGTCTGGGG 
AAACTCATCC ACTTGAAAGA CTGCGATCCC GATCAGATCT TCCTTGGTGG ACTGGACCAG 
TACGGAGACG ATGGAGAGTT CACATACTGC TGGCATGATG ACATTATGCA GGCTATTTTC 
CACATCGCCA CGCTGATGCC GAACCGTGAG AGCGACCGCG GCTGCTGCAA CAAGAAGCGG 
CACATCGGCA ACGACTTCGT GGTGGTGGTT TATAACGACT CGGGGGAGGA CTACAAACTG 
GGCACCATCA AGGGTCAGTT CAACTTTGTG GAAGTGCTCA TAAAGCCGCT GGACTACGAG 
AGTAACCTGG TGACACTGCA GTGCCGGAAA GATCTGGAGG GTTTGGTGGA CACCAGTGTA 
GCGAAGATCG TCTCTGACCG GAACCTTCCC CTGCTAGTCA GACAGATGGC GCTTCATGCT 
AACATGGCGT CGCTGGTCCA CCAGTGCAGG GCGAACCCAT CAGACGCATA CGCTTCAAAA 
TGGCTGGCCA GACTGAGACA CATCAAGAGG ATCAGGACGC GGGCTCAGGA GGAGATTCAG 
TCCCGCCCCT CTCACGGCAT CTCTCTGACT CAAGGCCACG CCTCCATGCA GAATAAACCC 
GCCCACCAGC CGCCGGGAGC AGCACAGAAT CCAGACGCCG GCCAGAGGAA GAGGCTGGTG 
TCCACCGTCG ACGACTTCAC TGACTTTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_021475594.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331269.1 Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa147277
Clone ID Related Accession (Same CDS sequence)	XM_021475594.1
Accession Version	XM_021475594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGCA CCATCCAGTA TAAAGTGCTG ATCCTGACGT CTCCATGTAA CATTGATAAT 
CTGTGCGCAA CGCTCTGCTC GATGGTGACG GACCGCATTA TTTTGGAGCG TCTGAAGAAA 
ACCCCTGATG GATTTTCCCT CACTGACGTT CAGCTCGCTG TGGTTCCTGT TCTCACGGCC 
ATCACGTCCT ACCACAGCTA TCTGGATCAG CTCCGACAGA GGGACCTGGT GCAGTGTCTG 
GAGAAGGGTC TGATCTACCG CTGTGCCAAG CAGTGTGTGG TGGCCCTGAC AATGTGCACA 
GTGGAGATGC CCGACATCAT GATCAAACTA CTGCCCGCCC TTATCGTCAA GCTCACGCAC 
ATCTCGGCAA CCGTTGCCAT GGCCTCACCC ATGCTGGAGT TCCTGTCCAC ATTAGTGCGG 
CTCCCACATC TGTATGCTAA CTTCGTAGCT GAGCAGTATG TTAGCGTGTT TGCCATATCG 
CTGCCGTACA CCAATCCTTC AAAGTTTAAT CAGTACATAG TGTCTCTGGC TCATCATGTG 
ATCGCCATGT GGTTCATCCG CTGCCGACTG CCTTTCAGGA AAGATTTTGT TCAGTACATC 
ACCAAGGGTC TTCGTTCTAA CGCTCTCCTG CCGTTTGACG ACACACACGA GCAGAGCAGT 
TTCAGAGCAC GCAGCACCAG CCTGAACGAG AGGCCCAAGA GTTTGCGGAC GACCAAAGTG 
GCGAAGCAAG GCCCCAGCGC AAACTCTCCC GTTAAAGACC TGAAAGACCT TTCGGCCATG 
GACGCCTTCC GCTCCCGCAG CATCAGTGTG TCCGAGCATG CTGTGCGCAG GATGCAGACG 
TCCAGCACCA CCTGCAGTCT GGGCTCAGCT GATGAGAACG CTGTGATGCA GGCGGACGAC 
GCTCTGAAGA CAGTCCATCT GGAGCTCACA GAGACCTGCC TGGACATGAT GGCCAGATAC 
GTCTTCTCCA ACTTCTCTGC TCTGCCCAAG AGGTCTCCCA TCGCTGATTT CCTGTTATCT 
GGTGGTCCTA GTATGACGTG GCTGGTTGGG AATAAGTTAG TGACCATAAC CACCAGCGGC 
GGCTCGCGAA CACAAGCTCT TCTGGGTCTG GACATGACAG AGAGACATGG AGGAGGAGAA 
ATGACCAGGT CTGACCCGTC TCTGCACACC CGACAGACCA AAGAAGCTCC CGCTAAACTA 
GAGTCTCAGT CCAGCCAACA GATCAACACC AACACACGCA CACGAGTCCG CTCTATATCA 
GGTGGTCATG CTCTGCGGGC CGGGCCAGCT CAGAGTCTTA GTCCTCTGGT GGCGTCTCCT 
GAAGGCGAGT ACAGCGGCCC GCTGCCTCCT CCCGGACCCC CGCTGGACGT CCTGAGCTCT 
CAGAGGGGGA TGGAGGAGCA CCCGCCACCC TCCGCCTGCA ACCCATCACC ACCGCTCAAG 
CACAAGCACA GCCTGGCAGA GTTTGTGCCC ATGCTGACGC AGGGCTGGGC CGAGATCTTC 
ATCCGCAGGC CCTCAGGGAA CACGAGCTGG CTGATGTGTC TGGAGAACCC GCCAAGCCCG 
TTCTCCTCTG AGCTGGGTAA TCTGCCTCTG CAGGAGCTCT CCAGCGTCCT CATGGCCATG 
GACGGAGTGA AGGAGCCACC GGCCGAGCGT CCCACAGACG TGTCCCAGCC ACAGGGCAAA 
CCGGCTCCCA TCCAGCGATC CAACACTGAT TCAGTAGTGA TGGAGGAAAG TGGGCGGAGT 
ATGGCGTCTG TTTCACCTGC TGAGTCTAAA GAGGTGGAGG AGTTTGAGGC CGTTCCTTCT 
GAACCCATCT TCATGAACAC CAGCACGACT GCACCCGGCA TGATGAGCAG ATCCTCCTCC 
ACATCCAGTC AGGATGATGA TAAGTCCACC CTGGAGGAGG TCAGTGAAGG AGGAATCCCC 
ATCGACCAGC CTCCGCTCAG CCTCTGCACC CCTGGAGCCC AGGACCCCGA GCTTTCCTTC 
ACCCAAAGCA GCAGCAGCTC CAGCACCCTT AATAAATCCA GCTCCTCTCC TGAGCTCCAG 
ACCCTTCCAG AGGCCTTCAG CAAAGCCTCG TCCCAGAGCG TGGATCCAGC TCCCACCAGC 
AAAATCCCTA CAGTCCAGGC CTGTGTGGAG GCTCCAGGAG AGAGCGCTGG ATCCACCGCT 
GGGACATCTT GCAGCAGCTC TAGCGTGTCC GTGTCCTCCT CCTCCACCTC CAGAATGAGG 
CTGGAGTTCC CGCCAGTGCA GACAGGACCC CTGTCTCCTA CTGGACACCG GCCACGGGGA 
CACACCATAT CAGTGTCTGC TCCATCGTCC AGGAGAGACC GCAAAACACT GGAGCGGGAC 
GCCAGAGGAG GAGCGACCAA TGTGGAGAAG AGCTCCGGAC TCAGCCCCAG TTTTGTCTTC 
CTGCAGCTTT ATCACTCGCC GTTCTTCGGC AATGAGGCCA ACAAACCCCT GCTGCTTCCT 
AAATCACAGC TGATTGACCG TGCAGTGAAG GTTTTGGATC AGATGCCTCC GTACGACACT 
CATAAGATCG GTGTGGTGTT TGTGGGAGCC GGACAGGCCA ATAATGAGGT GTCGATCCTG 
TCTAATGAGT ACGGCTCGAA GCGCTATGCT CAGTTCCTGA CGGGTCTGGG GAAACTCATC 
CACTTGAAAG ACTGCGATCC CGATCAGATC TTCCTTGGTG GACTGGACCA GTACGGAGAC 
GATGGAGAGT TCACATACTG CTGGCATGAT GACATTATGC AGGCTATTTT CCACATCGCC 
ACGCTGATGC CGAACCGTGA GAGCGACCGC GGCTGCTGCA ACAAGAAGCG GCACATCGGC 
AACGACTTCG TGGTGGTGGT TTATAACGAC TCGGGGGAGG ACTACAAACT GGGCACCATC 
AAGGGTCAGT TCAACTTTGT GGAAGTGCTC ATAAAGCCGC TGGACTACGA GAGTAACCTG 
GTGACACTGC AGTGCCGGAA AGATCTGGAG GGTTTGGTGG ACACCAGTGT AGCGAAGATC 
GTCTCTGACC GGAACCTTCC CCTGCTAGTC AGACAGATGG CGCTTCATGC TAACATGGCG 
TCGCTGGTCC ACCAGTGCAG GGCGAACCCA TCAGACGCAT ACGCTTCAAA ATGGCTGGCC 
AGACTGAGAC ACATCAAGAG GATCAGGACG CGGGCTCAGG AGGAGATTCA GTCCCGCCCC 
TCTCACGGCA TCTCTCTGAC TCAAGGCCAC GCCTCCATGC AGAATAAACC CGCCCACCAG 
CCGCCGGGAG CAGCACAGAA TCCAGACGCC GGCCAGAGGA AGAGGCTGGT GTCCACCGTC 
GACGACTTCA CTGACTTTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331269.1
CDS	163..3486
Translation	MSCTIQYKVLILTSPCNIDNLCATLCSMVTDRIILERLKKTPDGFSLTDVQLAVVPVLTAITSYHSYLDQLRQRDLVQCLEKGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDTHEQSSFRARSTSLNERPKSLRTTKVAKQGPSANSPVKDLKDLSAMDAFRSRSISVSEHAVRRMQTSSTTCSLGSADENAVMQADDALKTVHLELTETCLDMMARYVFSNFSALPKRSPIADFLLSGGPSMTWLVGNKLVTITTSGGSRTQALLGLDMTERHGGGEMTRSDPSLHTRQTKEAPAKLESQSSQQINTNTRTRVRSISGGHALRAGPAQSLSPLVASPEGEYSGPLPPPGPPLDVLSSQRGMEEHPPPSACNPSPPLKHKHSLAEFVPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNLPLQELSSVLMAMDGVKEPPAERPTDVSQPQGKPAPIQRSNTDSVVMEESGRSMASVSPAESKEVEEFEAVPSEPIFMNTSTTAPGMMSRSSSTSSQDDDKSTLEEVSEGGIPIDQPPLSLCTPGAQDPELSFTQSSSSSSTLNKSSSSPELQTLPEAFSKASSQSVDPAPTSKIPTVQACVEAPGESAGSTAGTSCSSSSVSVSSSSTSRMRLEFPPVQTGPLSPTGHRPRGHTISVSAPSSRRDRKTLERDARGGATNVEKSSGLSPSFVFLQLYHSPFFGNEANKPLLLPKSQLIDRAVKVLDQMPPYDTHKIGVVFVGAGQANNEVSILSNEYGSKRYAQFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDRGCCNKKRHIGNDFVVVVYNDSGEDYKLGTIKGQFNFVEVLIKPLDYESNLVTLQCRKDLEGLVDTSVAKIVSDRNLPLLVRQMALHANMASLVHQCRANPSDAYASKWLARLRHIKRIRTRAQEEIQSRPSHGISLTQGHASMQNKPAHQPPGAAQNPDAGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_021475594.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475594.1

ORF Insert Sequence:

ATGTCCTGCA CCATCCAGTA TAAAGTGCTG ATCCTGACGT CTCCATGTAA CATTGATAAT 
CTGTGCGCAA CGCTCTGCTC GATGGTGACG GACCGCATTA TTTTGGAGCG TCTGAAGAAA 
ACCCCTGATG GATTTTCCCT CACTGACGTT CAGCTCGCTG TGGTTCCTGT TCTCACGGCC 
ATCACGTCCT ACCACAGCTA TCTGGATCAG CTCCGACAGA GGGACCTGGT GCAGTGTCTG 
GAGAAGGGTC TGATCTACCG CTGTGCCAAG CAGTGTGTGG TGGCCCTGAC AATGTGCACA 
GTGGAGATGC CCGACATCAT GATCAAACTA CTGCCCGCCC TTATCGTCAA GCTCACGCAC 
ATCTCGGCAA CCGTTGCCAT GGCCTCACCC ATGCTGGAGT TCCTGTCCAC ATTAGTGCGG 
CTCCCACATC TGTATGCTAA CTTCGTAGCT GAGCAGTATG TTAGCGTGTT TGCCATATCG 
CTGCCGTACA CCAATCCTTC AAAGTTTAAT CAGTACATAG TGTCTCTGGC TCATCATGTG 
ATCGCCATGT GGTTCATCCG CTGCCGACTG CCTTTCAGGA AAGATTTTGT TCAGTACATC 
ACCAAGGGTC TTCGTTCTAA CGCTCTCCTG CCGTTTGACG ACACACACGA GCAGAGCAGT 
TTCAGAGCAC GCAGCACCAG CCTGAACGAG AGGCCCAAGA GTTTGCGGAC GACCAAAGTG 
GCGAAGCAAG GCCCCAGCGC AAACTCTCCC GTTAAAGACC TGAAAGACCT TTCGGCCATG 
GACGCCTTCC GCTCCCGCAG CATCAGTGTG TCCGAGCATG CTGTGCGCAG GATGCAGACG 
TCCAGCACCA CCTGCAGTCT GGGCTCAGCT GATGAGAACG CTGTGATGCA GGCGGACGAC 
GCTCTGAAGA CAGTCCATCT GGAGCTCACA GAGACCTGCC TGGACATGAT GGCCAGATAC 
GTCTTCTCCA ACTTCTCTGC TCTGCCCAAG AGGTCTCCCA TCGCTGATTT CCTGTTATCT 
GGTGGTCCTA GTATGACGTG GCTGGTTGGG AATAAGTTAG TGACCATAAC CACCAGCGGC 
GGCTCGCGAA CACAAGCTCT TCTGGGTCTG GACATGACAG AGAGACATGG AGGAGGAGAA 
ATGACCAGGT CTGACCCGTC TCTGCACACC CGACAGACCA AAGAAGCTCC CGCTAAACTA 
GAGTCTCAGT CCAGCCAACA GATCAACACC AACACACGCA CACGAGTCCG CTCTATATCA 
GGTGGTCATG CTCTGCGGGC CGGGCCAGCT CAGAGTCTTA GTCCTCTGGT GGCGTCTCCT 
GAAGGCGAGT ACAGCGGCCC GCTGCCTCCT CCCGGACCCC CGCTGGACGT CCTGAGCTCT 
CAGAGGGGGA TGGAGGAGCA CCCGCCACCC TCCGCCTGCA ACCCATCACC ACCGCTCAAG 
CACAAGCACA GCCTGGCAGA GTTTGTGCCC ATGCTGACGC AGGGCTGGGC CGAGATCTTC 
ATCCGCAGGC CCTCAGGGAA CACGAGCTGG CTGATGTGTC TGGAGAACCC GCCAAGCCCG 
TTCTCCTCTG AGCTGGGTAA TCTGCCTCTG CAGGAGCTCT CCAGCGTCCT CATGGCCATG 
GACGGAGTGA AGGAGCCACC GGCCGAGCGT CCCACAGACG TGTCCCAGCC ACAGGGCAAA 
CCGGCTCCCA TCCAGCGATC CAACACTGAT TCAGTAGTGA TGGAGGAAAG TGGGCGGAGT 
ATGGCGTCTG TTTCACCTGC TGAGTCTAAA GAGGTGGAGG AGTTTGAGGC CGTTCCTTCT 
GAACCCATCT TCATGAACAC CAGCACGACT GCACCCGGCA TGATGAGCAG ATCCTCCTCC 
ACATCCAGTC AGGATGATGA TAAGTCCACC CTGGAGGAGG TCAGTGAAGG AGGAATCCCC 
ATCGACCAGC CTCCGCTCAG CCTCTGCACC CCTGGAGCCC AGGACCCCGA GCTTTCCTTC 
ACCCAAAGCA GCAGCAGCTC CAGCACCCTT AATAAATCCA GCTCCTCTCC TGAGCTCCAG 
ACCCTTCCAG AGGCCTTCAG CAAAGCCTCG TCCCAGAGCG TGGATCCAGC TCCCACCAGC 
AAAATCCCTA CAGTCCAGGC CTGTGTGGAG GCTCCAGGAG AGAGCGCTGG ATCCACCGCT 
GGGACATCTT GCAGCAGCTC TAGCGTGTCC GTGTCCTCCT CCTCCACCTC CAGAATGAGG 
CTGGAGTTCC CGCCAGTGCA GACAGGACCC CTGTCTCCTA CTGGACACCG GCCACGGGGA 
CACACCATAT CAGTGTCTGC TCCATCGTCC AGGAGAGACC GCAAAACACT GGAGCGGGAC 
GCCAGAGGAG GAGCGACCAA TGTGGAGAAG AGCTCCGGAC TCAGCCCCAG TTTTGTCTTC 
CTGCAGCTTT ATCACTCGCC GTTCTTCGGC AATGAGGCCA ACAAACCCCT GCTGCTTCCT 
AAATCACAGC TGATTGACCG TGCAGTGAAG GTTTTGGATC AGATGCCTCC GTACGACACT 
CATAAGATCG GTGTGGTGTT TGTGGGAGCC GGACAGGCCA ATAATGAGGT GTCGATCCTG 
TCTAATGAGT ACGGCTCGAA GCGCTATGCT CAGTTCCTGA CGGGTCTGGG GAAACTCATC 
CACTTGAAAG ACTGCGATCC CGATCAGATC TTCCTTGGTG GACTGGACCA GTACGGAGAC 
GATGGAGAGT TCACATACTG CTGGCATGAT GACATTATGC AGGCTATTTT CCACATCGCC 
ACGCTGATGC CGAACCGTGA GAGCGACCGC GGCTGCTGCA ACAAGAAGCG GCACATCGGC 
AACGACTTCG TGGTGGTGGT TTATAACGAC TCGGGGGAGG ACTACAAACT GGGCACCATC 
AAGGGTCAGT TCAACTTTGT GGAAGTGCTC ATAAAGCCGC TGGACTACGA GAGTAACCTG 
GTGACACTGC AGTGCCGGAA AGATCTGGAG GGTTTGGTGG ACACCAGTGT AGCGAAGATC 
GTCTCTGACC GGAACCTTCC CCTGCTAGTC AGACAGATGG CGCTTCATGC TAACATGGCG 
TCGCTGGTCC ACCAGTGCAG GGCGAACCCA TCAGACGCAT ACGCTTCAAA ATGGCTGGCC 
AGACTGAGAC ACATCAAGAG GATCAGGACG CGGGCTCAGG AGGAGATTCA GTCCCGCCCC 
TCTCACGGCA TCTCTCTGAC TCAAGGCCAC GCCTCCATGC AGAATAAACC CGCCCACCAG 
CCGCCGGGAG CAGCACAGAA TCCAGACGCC GGCCAGAGGA AGAGGCTGGT GTCCACCGTC 
GACGACTTCA CTGACTTTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_021475598.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331273.1 Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X3, mRNA.

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CloneID	ODa147278
Clone ID Related Accession (Same CDS sequence)	XM_021475598.1
Accession Version	XM_021475598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGCA CCATCCAGTA TAAAGTGCTG ATCCTGACGT CTCCATGTAA CATTGATAAT 
CTGTGCGCAA CGCTCTGCTC GATGGTGACG GACCGCATTA TTTTGGAGCG TCTGAAGAAA 
ACCCCTGATG GATTTTCCCT CACTGACGTT CAGCTCGCTG TGGTTCCTGT TCTCACGGCC 
ATCACGTCCT ACCACAGCTA TCTGGATCAG CTCCGACAGA GGGACCTGGT GCAGTGTCTG 
GAGAAGGGTC TGATCTACCG CTGTGCCAAG CAGTGTGTGG TGGCCCTGAC AATGTGCACA 
GTGGAGATGC CCGACATCAT GATCAAACTA CTGCCCGCCC TTATCGTCAA GCTCACGCAC 
ATCTCGGCAA CCGTTGCCAT GGCCTCACCC ATGCTGGAGT TCCTGTCCAC ATTAGTGCGG 
CTCCCACATC TGTATGCTAA CTTCGTAGCT GAGCAGTATG TTAGCGTGTT TGCCATATCG 
CTGCCGTACA CCAATCCTTC AAAGTTTAAT CAGTACATAG TGTCTCTGGC TCATCATGTG 
ATCGCCATGT GGTTCATCCG CTGCCGACTG CCTTTCAGGA AAGATTTTGT TCAGTACATC 
ACCAAGGGTC TTCGTTCTAA CGCTCTCCTG CCGTTTGACG ACACACACGA GCAGAGCAGT 
TTCAGAGCAC GCAGCACCAG CCTGAACGAG AGGCCCAAGA GGATGCAGAC GTCCAGCACC 
ACCTGCAGTC TGGGCTCAGC TGATGAGAAC GCTGTGATGC AGGCGGACGA CGCTCTGAAG 
ACAGTCCATC TGGAGCTCAC AGAGACCTGC CTGGACATGA TGGCCAGATA CGTCTTCTCC 
AACTTCTCTG CTCTGCCCAA GAGGTCTCCC ATCGCTGATT TCCTGTTATC TGGTGGTCCT 
AGTATGACGT GGCTGGTTGG GAATAAGTTA GTGACCATAA CCACCAGCGG CGGCTCGCGA 
ACACAAGCTC TTCTGGGTCT GGACATGACA GAGAGACATG GAGGAGGAGA AATGACCAGG 
TCTGACCCGT CTCTGCACAC CCGACAGACC AAAGAAGCTC CCGCTAAACT AGAGTCTCAG 
TCCAGCCAAC AGATCAACAC CAACACACGC ACACGAGTCC GCTCTATATC AGGTGGTCAT 
GCTCTGCGGG CCGGGCCAGC TCAGAGTCTT AGTCCTCTGG TGGCGTCTCC TGAAGGCGAG 
TACAGCGGCC CGCTGCCTCC TCCCGGACCC CCGCTGGACG TCCTGAGCTC TCAGAGGGGG 
ATGGAGGAGC ACCCGCCACC CTCCGCCTGC AACCCATCAC CACCGCTCAA GCACAAGCAC 
AGCCTGGCAG AGTTTGTGCC CATGCTGACG CAGGGCTGGG CCGAGATCTT CATCCGCAGG 
CCCTCAGGGA ACACGAGCTG GCTGATGTGT CTGGAGAACC CGCCAAGCCC GTTCTCCTCT 
GAGCTGGGTA ATCTGCCTCT GCAGGAGCTC TCCAGCGTCC TCATGGCCAT GGACGGAGTG 
AAGGAGCCAC CGGCCGAGCG TCCCACAGAC GTGTCCCAGC CACAGGGCAA ACCGGCTCCC 
ATCCAGCGAT CCAACACTGT GGCCTCTCTC AGCTCTCTCT GCTCCTCTGA ATCATCCGGC 
AGGTTTCACA GGAGTATATC CTGGGCAGAT TCAGTAGTGA TGGAGGAAAG TGGGCGGAGT 
ATGGCGTCTG TTTCACCTGC TGAGTCTAAA GAGGTGGAGG AGTTTGAGGC CGTTCCTTCT 
GAACCCATCT TCATGAACAC CAGCACGACT GCACCCGGCA TGATGAGCAG ATCCTCCTCC 
ACATCCAGTC AGGATGATGA TAAGTCCACC CTGGAGGAGG TCAGTGAAGG AGGAATCCCC 
ATCGACCAGC CTCCGCTCAG CCTCTGCACC CCTGGAGCCC AGGACCCCGA GCTTTCCTTC 
ACCCAAAGCA GCAGCAGCTC CAGCACCCTT AATAAATCCA GCTCCTCTCC TGAGCTCCAG 
ACCCTTCCAG AGGCCTTCAG CAAAGCCTCG TCCCAGAGCG TGGATCCAGC TCCCACCAGC 
AAAATCCCTA CAGTCCAGGC CTGTGTGGAG GCTCCAGGAG AGAGCGCTGG ATCCACCGCT 
GGGACATCTT GCAGCAGCTC TAGCGTGTCC GTGTCCTCCT CCTCCACCTC CAGAATGAGG 
CTGGAGTTCC CGCCAGTGCA GACAGGACCC CTGTCTCCTA CTGGACACCG GCCACGGGGA 
CACACCATAT CAGTGTCTGC TCCATCGTCC AGGAGAGACC GCAAAACACT GGAGCGGGAC 
GCCAGAGGAG GAGCGACCAA TGTGGAGAAG AGCTCCGGAC TCAGCCCCAG TTTTGTCTTC 
CTGCAGCTTT ATCACTCGCC GTTCTTCGGC AATGAGGCCA ACAAACCCCT GCTGCTTCCT 
AAATCACAGC TGATTGACCG TGCAGTGAAG GTTTTGGATC AGATGCCTCC GTACGACACT 
CATAAGATCG GTGTGGTGTT TGTGGGAGCC GGACAGGCCA ATAATGAGGT GTCGATCCTG 
TCTAATGAGT ACGGCTCGAA GCGCTATGCT CAGTTCCTGA CGGGTCTGGG GAAACTCATC 
CACTTGAAAG ACTGCGATCC CGATCAGATC TTCCTTGGTG GACTGGACCA GTACGGAGAC 
GATGGAGAGT TCACATACTG CTGGCATGAT GACATTATGC AGGCTATTTT CCACATCGCC 
ACGCTGATGC CGAACCGTGA GAGCGACCGC GGCTGCTGCA ACAAGAAGCG GCACATCGGC 
AACGACTTCG TGGTGGTGGT TTATAACGAC TCGGGGGAGG ACTACAAACT GGGCACCATC 
AAGGGTCAGT TCAACTTTGT GGAAGTGCTC ATAAAGCCGC TGGACTACGA GAGTAACCTG 
GTGACACTGC AGTGCCGGAA AGATCTGGAG GGTTTGGTGG ACACCAGTGT AGCGAAGATC 
GTCTCTGACC GGAACCTTCC CCTGCTAGTC AGACAGATGG CGCTTCATGC TAACATGGCG 
TCGCTGGTCC ACCAGTGCAG GGCGAACCCA TCAGACGCAT ACGCTTCAAA ATGGCTGGCC 
AGACTGAGAC ACATCAAGAG GATCAGGACG CGGGCTCAGG AGGAGATTCA GTCCCGCCCC 
TCTCACGGCA TCTCTCTGAC TCAAGGCCAC GCCTCCATGC AGAATAAACC CGCCCACCAG 
CCGCCGGGAG CAGCACAGAA TCCAGACGCC GGCCAGAGGA AGAGGCTGGT GTCCACCGTC 
GACGACTTCA CTGACTTTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331273.1
CDS	163..3426
Translation	MSCTIQYKVLILTSPCNIDNLCATLCSMVTDRIILERLKKTPDGFSLTDVQLAVVPVLTAITSYHSYLDQLRQRDLVQCLEKGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDTHEQSSFRARSTSLNERPKRMQTSSTTCSLGSADENAVMQADDALKTVHLELTETCLDMMARYVFSNFSALPKRSPIADFLLSGGPSMTWLVGNKLVTITTSGGSRTQALLGLDMTERHGGGEMTRSDPSLHTRQTKEAPAKLESQSSQQINTNTRTRVRSISGGHALRAGPAQSLSPLVASPEGEYSGPLPPPGPPLDVLSSQRGMEEHPPPSACNPSPPLKHKHSLAEFVPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNLPLQELSSVLMAMDGVKEPPAERPTDVSQPQGKPAPIQRSNTVASLSSLCSSESSGRFHRSISWADSVVMEESGRSMASVSPAESKEVEEFEAVPSEPIFMNTSTTAPGMMSRSSSTSSQDDDKSTLEEVSEGGIPIDQPPLSLCTPGAQDPELSFTQSSSSSSTLNKSSSSPELQTLPEAFSKASSQSVDPAPTSKIPTVQACVEAPGESAGSTAGTSCSSSSVSVSSSSTSRMRLEFPPVQTGPLSPTGHRPRGHTISVSAPSSRRDRKTLERDARGGATNVEKSSGLSPSFVFLQLYHSPFFGNEANKPLLLPKSQLIDRAVKVLDQMPPYDTHKIGVVFVGAGQANNEVSILSNEYGSKRYAQFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDRGCCNKKRHIGNDFVVVVYNDSGEDYKLGTIKGQFNFVEVLIKPLDYESNLVTLQCRKDLEGLVDTSVAKIVSDRNLPLLVRQMALHANMASLVHQCRANPSDAYASKWLARLRHIKRIRTRAQEEIQSRPSHGISLTQGHASMQNKPAHQPPGAAQNPDAGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_021475598.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475598.1

ORF Insert Sequence:

ATGTCCTGCA CCATCCAGTA TAAAGTGCTG ATCCTGACGT CTCCATGTAA CATTGATAAT 
CTGTGCGCAA CGCTCTGCTC GATGGTGACG GACCGCATTA TTTTGGAGCG TCTGAAGAAA 
ACCCCTGATG GATTTTCCCT CACTGACGTT CAGCTCGCTG TGGTTCCTGT TCTCACGGCC 
ATCACGTCCT ACCACAGCTA TCTGGATCAG CTCCGACAGA GGGACCTGGT GCAGTGTCTG 
GAGAAGGGTC TGATCTACCG CTGTGCCAAG CAGTGTGTGG TGGCCCTGAC AATGTGCACA 
GTGGAGATGC CCGACATCAT GATCAAACTA CTGCCCGCCC TTATCGTCAA GCTCACGCAC 
ATCTCGGCAA CCGTTGCCAT GGCCTCACCC ATGCTGGAGT TCCTGTCCAC ATTAGTGCGG 
CTCCCACATC TGTATGCTAA CTTCGTAGCT GAGCAGTATG TTAGCGTGTT TGCCATATCG 
CTGCCGTACA CCAATCCTTC AAAGTTTAAT CAGTACATAG TGTCTCTGGC TCATCATGTG 
ATCGCCATGT GGTTCATCCG CTGCCGACTG CCTTTCAGGA AAGATTTTGT TCAGTACATC 
ACCAAGGGTC TTCGTTCTAA CGCTCTCCTG CCGTTTGACG ACACACACGA GCAGAGCAGT 
TTCAGAGCAC GCAGCACCAG CCTGAACGAG AGGCCCAAGA GGATGCAGAC GTCCAGCACC 
ACCTGCAGTC TGGGCTCAGC TGATGAGAAC GCTGTGATGC AGGCGGACGA CGCTCTGAAG 
ACAGTCCATC TGGAGCTCAC AGAGACCTGC CTGGACATGA TGGCCAGATA CGTCTTCTCC 
AACTTCTCTG CTCTGCCCAA GAGGTCTCCC ATCGCTGATT TCCTGTTATC TGGTGGTCCT 
AGTATGACGT GGCTGGTTGG GAATAAGTTA GTGACCATAA CCACCAGCGG CGGCTCGCGA 
ACACAAGCTC TTCTGGGTCT GGACATGACA GAGAGACATG GAGGAGGAGA AATGACCAGG 
TCTGACCCGT CTCTGCACAC CCGACAGACC AAAGAAGCTC CCGCTAAACT AGAGTCTCAG 
TCCAGCCAAC AGATCAACAC CAACACACGC ACACGAGTCC GCTCTATATC AGGTGGTCAT 
GCTCTGCGGG CCGGGCCAGC TCAGAGTCTT AGTCCTCTGG TGGCGTCTCC TGAAGGCGAG 
TACAGCGGCC CGCTGCCTCC TCCCGGACCC CCGCTGGACG TCCTGAGCTC TCAGAGGGGG 
ATGGAGGAGC ACCCGCCACC CTCCGCCTGC AACCCATCAC CACCGCTCAA GCACAAGCAC 
AGCCTGGCAG AGTTTGTGCC CATGCTGACG CAGGGCTGGG CCGAGATCTT CATCCGCAGG 
CCCTCAGGGA ACACGAGCTG GCTGATGTGT CTGGAGAACC CGCCAAGCCC GTTCTCCTCT 
GAGCTGGGTA ATCTGCCTCT GCAGGAGCTC TCCAGCGTCC TCATGGCCAT GGACGGAGTG 
AAGGAGCCAC CGGCCGAGCG TCCCACAGAC GTGTCCCAGC CACAGGGCAA ACCGGCTCCC 
ATCCAGCGAT CCAACACTGT GGCCTCTCTC AGCTCTCTCT GCTCCTCTGA ATCATCCGGC 
AGGTTTCACA GGAGTATATC CTGGGCAGAT TCAGTAGTGA TGGAGGAAAG TGGGCGGAGT 
ATGGCGTCTG TTTCACCTGC TGAGTCTAAA GAGGTGGAGG AGTTTGAGGC CGTTCCTTCT 
GAACCCATCT TCATGAACAC CAGCACGACT GCACCCGGCA TGATGAGCAG ATCCTCCTCC 
ACATCCAGTC AGGATGATGA TAAGTCCACC CTGGAGGAGG TCAGTGAAGG AGGAATCCCC 
ATCGACCAGC CTCCGCTCAG CCTCTGCACC CCTGGAGCCC AGGACCCCGA GCTTTCCTTC 
ACCCAAAGCA GCAGCAGCTC CAGCACCCTT AATAAATCCA GCTCCTCTCC TGAGCTCCAG 
ACCCTTCCAG AGGCCTTCAG CAAAGCCTCG TCCCAGAGCG TGGATCCAGC TCCCACCAGC 
AAAATCCCTA CAGTCCAGGC CTGTGTGGAG GCTCCAGGAG AGAGCGCTGG ATCCACCGCT 
GGGACATCTT GCAGCAGCTC TAGCGTGTCC GTGTCCTCCT CCTCCACCTC CAGAATGAGG 
CTGGAGTTCC CGCCAGTGCA GACAGGACCC CTGTCTCCTA CTGGACACCG GCCACGGGGA 
CACACCATAT CAGTGTCTGC TCCATCGTCC AGGAGAGACC GCAAAACACT GGAGCGGGAC 
GCCAGAGGAG GAGCGACCAA TGTGGAGAAG AGCTCCGGAC TCAGCCCCAG TTTTGTCTTC 
CTGCAGCTTT ATCACTCGCC GTTCTTCGGC AATGAGGCCA ACAAACCCCT GCTGCTTCCT 
AAATCACAGC TGATTGACCG TGCAGTGAAG GTTTTGGATC AGATGCCTCC GTACGACACT 
CATAAGATCG GTGTGGTGTT TGTGGGAGCC GGACAGGCCA ATAATGAGGT GTCGATCCTG 
TCTAATGAGT ACGGCTCGAA GCGCTATGCT CAGTTCCTGA CGGGTCTGGG GAAACTCATC 
CACTTGAAAG ACTGCGATCC CGATCAGATC TTCCTTGGTG GACTGGACCA GTACGGAGAC 
GATGGAGAGT TCACATACTG CTGGCATGAT GACATTATGC AGGCTATTTT CCACATCGCC 
ACGCTGATGC CGAACCGTGA GAGCGACCGC GGCTGCTGCA ACAAGAAGCG GCACATCGGC 
AACGACTTCG TGGTGGTGGT TTATAACGAC TCGGGGGAGG ACTACAAACT GGGCACCATC 
AAGGGTCAGT TCAACTTTGT GGAAGTGCTC ATAAAGCCGC TGGACTACGA GAGTAACCTG 
GTGACACTGC AGTGCCGGAA AGATCTGGAG GGTTTGGTGG ACACCAGTGT AGCGAAGATC 
GTCTCTGACC GGAACCTTCC CCTGCTAGTC AGACAGATGG CGCTTCATGC TAACATGGCG 
TCGCTGGTCC ACCAGTGCAG GGCGAACCCA TCAGACGCAT ACGCTTCAAA ATGGCTGGCC 
AGACTGAGAC ACATCAAGAG GATCAGGACG CGGGCTCAGG AGGAGATTCA GTCCCGCCCC 
TCTCACGGCA TCTCTCTGAC TCAAGGCCAC GCCTCCATGC AGAATAAACC CGCCCACCAG 
CCGCCGGGAG CAGCACAGAA TCCAGACGCC GGCCAGAGGA AGAGGCTGGT GTCCACCGTC 
GACGACTTCA CTGACTTTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( NM_001328401.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315330.1 Danio rerio TSC complex subunit 2 (tsc2), mRNA.

Price & Availability↑

CloneID	ODa60579
Clone ID Related Accession (Same CDS sequence)	NM_001328401.1
Accession Version	NM_001328401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-28
Organism	Danio rerio(zebrafish)
Product	tuberin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01113995.1, CABZ01113996.1, CABZ01043817.1, CABZ01043818.1, FO681319.1 and FQ311887.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAACA GCATGTCCTC AGATTTCCTG CACGTCCTCG TCAACCTTGT CAAATTCAAC 
TCCTGCTACC TGGATGAGAA CGTTTCGCTT ATGGTCCAGA AAATCTGCCT TCTGTGCAAC 
CGGACGACAT CCTCCACAGA CATTGAGGTT GCGCTGCAGG TGCTGGACGC GGTGGTGTGT 
TATAACTGTC TGCCATCAGA CTCTCTAACA GTCTTCATCA TCACTCTGTG CCGAACTGTA 
AATGTAAAGG AGTTCTGCGA GTCCTGCTGG AAGTTGATGC GCAAAGTGTT GGGAACTCAT 
CTGGGTCACA GTGCCATATA CACCATGTGC CGAATCATGG AGGAGAGGGT GTACTCCGAG 
GACGCCGCTC TCCTCCGCGG TGCTGTGTTT TTTGTTGGGA TGGCGCTGTG GGGCGCTCAC 
AGACTCCCCG CCCTCAAAAA CACACCTACG CTGGTCCTGC CTTCCTTCTA CAAGGCCATG 
AGCTGTGCTA ATGAGGTGGT GTCCTATGAG ATCGTGCTCT CCATCACGCG CCTCATTAAA 
AAATACGGCC GCGAGCTGCA GGTGGTGACC TGGGACATCC TGCTGTCCAT CATAGACAGA 
CTCCTGCAGC AGATACAGAC GATGGGCAGT CCTGACCTGA AGGTGATCGT CTATGAGCTG 
CTGAGCACCA TCGAGGAGCT TTACGAGCAG AACGACTTCC ACGGCTCCTC GGCCCGCTTC 
TTCAGCCTGG TGGAGAAGTG TGCGGATAAA CGTCCTGATG CATCTGTGCT CACGCTGGTC 
TCCTACAGAG CTCAGGCCAT CCAGCCGGCC AAGGACGGAT GGCTGCAGAA CCTGCTCAAA 
CTCATGGACA AGTTCTTCAG GAATGAGAGC CGCACTATGA TCAGGATCAA GGTTCTGCAC 
ATCTTGTCGT TCGTCCTCAG CACAAACCGT CAGCTGTATG AGGAGGAGCT GATTGAGGTG 
GTGGTCATCC CTCAGCTGGG TCAGATAGCT GAGGATCGTG ACCCGTCAGT CAGGAAGCAG 
GCCACACAGC TGCTGGTGGA TCTGGCCGAG GGCTGCAGCA CACACCACTT CAGCAGTCTG 
CTGGACATCA TCGAGAAGGT GGCCAGTCGT CCTCTGAGCT GCTCTGTAGA GGGCGACAGA 
GAACTGTGTG TGGAGTCGCC GCTGGAGGAC GTGCGCACCG CTATACTGGG CCTGCTGGAC 
ATCCTGCAGA GCAAACTCTA TAGTCTGCCT GCCAGTCATG CCAGTCGAGT GTATGAGCTG 
CTCATCTCAC ACCTGCAGCT GCACTACAAG AACAAATACT GCAGCGCCAT CAGCGGAGCC 
ATACGCCTCA AGGTGTTTGA TTTCCTGCTG CTGATGAGGG CTGATTCTCT ACATCGTCTC 
GGGGTCCCCA ATAAAGACGG AGCTCTGAGA TTCAGCCCGT ACTGCCACTG TGACCCAGGG 
GAGTCCGAGA AGAGAGTGTC AGAGAAGAAG CCGACAGGAA GTGTGTCTCC ACCGGCAGGA 
AGTCCCACAC CGGCAGCTCC GCCCTCCTCC ATCCGCACCG CTTACCTGCC CTACAGTCTG 
GCTTTCAGTG TGCTGCTGCA GTGCCTCAAG ACGGAGACCG ACTGGAAGGT CCTGAAGCTG 
GTTCTGGATA AGATGTCCTG CACCATCCAG TATAAAGTGC TGATCCTGAC GTCTCCATGT 
AACATTGATA ATCTGTGCGC AACGCTCTGC TCGATGGTGA CGGACCGCAT TATTTTGGAG 
CGTCTGAAGA AAACCCCTGA TGGATTTTCC CTCACTGACG TTCAGCTCGC TGTGGTTCCT 
GTTCTCACGG CCATCACGTC CTACCACAGC TATCTGGATC AGCTCCGACA GAGGGACCTG 
GTGCAGTGTC TGGAGAAGGG TCTGATCTAC CGCTGTGCCA AGCAGTGTGT GGTGGCCCTG 
ACAATGTGCA CAGTGGAGAT GCCCGACATC ATGATCAAAC TACTGCCCGC CCTTATCGTC 
AAGCTCACGC ACATCTCGGC AACCGTTGCC ATGGCCTCAC CCATGCTGGA GTTCCTGTCC 
ACATTAGTGC GGCTCCCACA TCTGTATGCT AACTTCGTAG CTGAGCAGTA TGTTAGCGTG 
TTTGCCATAT CGCTGCCGTA CACCAATCCT TCAAAGTTTA ATCAGTACAT AGTGTCTCTG 
GCTCATCATG TGATCGCCAT GTGGTTCATC CGCTGCCGAC TGCCTTTCAG GAAAGATTTT 
GTTCAGTACA TCACCAAGGG TCTTCGTTCT AACGCTCTCC TGCCGTTTGA CGACACACAC 
GAGCAGAGCA GTTTCAGAGC ACGCAGCACC AGCCTGAACG AGAGGCCCAA GAGTTTGCGG 
ACGACCAAAG TGGCGAAGCA AGGCCCCAGC GCAAACTCTC CCGTTAAAGA CCTGAAAGAC 
CTTTCGGCCA TGGACGCCTT CCGCTCCCGC AGCATCAGTG TGTCCGAGCA TGCTGTGCGC 
AGGATGCAGA CGTCCAGCAC CACCTGCAGT CTGGGCTCAG CTGATGAGAA CGCTGTGATG 
CAGGCGGACG ACGCTCTGAA GACAGTCCAT CTGGAGCTCA CAGAGACCTG CCTGGACATG 
ATGGCCAGAT ACGTCTTCTC CAACTTCTCT GCTCTGCCCA AGAGGTCTCC CATCGCTGAT 
TTCCTGTTAT CTGGTGGTCC TAGTATGACG TGGCTGGTTG GGAATAAGTT AGTGACCATA 
ACCACCAGCG GCGGCTCGCG AACACAAGCT CTTCTGGGTC TGGACATGAC AGAGAGACAT 
GGAGGAGGAG AAATGACCAG GTCTGACCCG TCTCTGCACA CCCGACAGAC CAAAGAAGCT 
CCCGCTAAAC TAGAGTCTCA GTCCAGCCAA CAGATCAACA CCAACACACG CACACGAGTC 
CGCTCTATAT CAGGTGGTCA TGCTCTGCGG GCCGGGCCAG CTCAGAGTCT TAGTCCTCTG 
GTGGCGTCTC CTGAAGGCGA GTACAGCGGC CCGCTGCCTC CTCCCGGACC CCCGCTGGAC 
GTCCTGAGCT CTCAGAGGGG GATGGAGGAG CACCCGCCAC CCTCCGCCTG CAACCCATCA 
CCACCGCTCA AGCACAAGCA CAGCCTGGCA GAGTTTGTGC CCATGCTGAC GCAGGGCTGG 
GCCGAGATCT TCATCCGCAG GCCCTCAGGG AACACGAGCT GGCTGATGTG TCTGGAGAAC 
CCGCCAAGCC CGTTCTCCTC TGAGCTGGGT AATCTGCCTC TGCAGGAGCT CTCCAGCGTC 
CTCATGGCCA TGGACGGAGT GAAGGAGCCA CCGGCCGAGC GTCCCACAGA CGTGTCCCAG 
CCACAGGGCA AACCGGCTCC CATCCAGCGA TCCAACACTG TGGCCTCTCT CAGCTCTCTC 
TGCTCCTCTG AATCATCCGG CAGGTTTCAC AGGAGTATAT CCTGGGCAGA TTCAGTAGTG 
ATGGAGGAAA GTGGGCGGAG TATGGCGTCT GTTTCACCTG CTGAGTCTAA AGAGGTGGAG 
GAGTTTGAGG CCGTTCCTTC TGAACCCATC TTCATGAACA CCAGCACGAC TGCACCCGGC 
ATGATGAGCA GATCCTCCTC CACATCCAGT CAGGATGATG ATAAGTCCAC CCTGGAGGAG 
GTCAGTGAAG GAGGAATCCC CATCGACCAG CCTCCGCTCA GCCTCTGCAC CCCTGGAGCC 
CAGGACCCCG AGCTTTCCTT CACCCAAAGC AGCAGCAGCT CCAGCACCCT TAATAAATCC 
AGCTCCTCTC CTGAGCTCCA GACCCTTCCA GAGGCCTTCA GCAAAGCCTC GTCCCAGAGC 
GTGGATCCAG CTCCCACCAG CAAAATCCCT ACAGTCCAGG CCTGTGTGGA GGCTCCAGGA 
GAGAGCGCTG GATCCACCGC TGGGACATCT TGCAGCAGCT CTAGCGTGTC CGTGTCCTCC 
TCCTCCACCT CCAGAATGAG GCTGGAGTTC CCGCCAGTGC AGACAGGACC CCTGTCTCCT 
ACTGGACACC GGCCACGGGG ACACACCATA TCAGTGTCTG CTCCATCGTC CAGGAGAGAC 
CGCAAAACAC TGGAGCGGGA CGCCAGAGGA GGAGCGACCA ATGTGGAGAA GAGCTCCGGA 
CTCAGCCCCA GTTTTGTCTT CCTGCAGCTT TATCACTCGC CGTTCTTCGG CAATGAGGCC 
AACAAACCCC TGCTGCTTCC TAAATCACAG CTGATTGACC GTGCAGTGAA GGTTTTGGAT 
CAGATGCCTC CGTACGACAC TCATAAGATC GGTGTGGTGT TTGTGGGAGC CGGACAGGCC 
AATAATGAGG TGTCGATCCT GTCTAATGAG TACGGCTCGA AGCGCTATGC TCAGTTCCTG 
ACGGGTCTGG GGAAACTCAT CCACTTGAAA GACTGCGATC CCGATCAGAT CTTCCTTGGT 
GGACTGGACC AGTACGGAGA CGATGGAGAG TTCACATACT GCTGGCATGA TGACATTATG 
CAGGCTATTT TCCACATCGC CACGCTGATG CCGAACCGTG AGAGCGACCG CGGCTGCTGC 
AACAAGAAGC GGCACATCGG CAACGACTTC GTGGTGGTGG TTTATAACGA CTCGGGGGAG 
GACTACAAAC TGGGCACCAT CAAGGGTCAG TTCAACTTTG TGGAAGTGCT CATAAAGCCG 
CTGGACTACG AGAGTAACCT GGTGACACTG CAGTGCCGGA AAGATCTGGA GGGTTTGGTG 
GACACCAGTG TAGCGAAGAT CGTCTCTGAC CGGAACCTTC CCCTGCTAGT CAGACAGATG 
GCGCTTCATG CTAACATGGC GTCGCTGGTC CACCAGTGCA GGGCGAACCC ATCAGACGCA 
TACGCTTCAA AATGGCTGGC CAGACTGAGA CACATCAAGA GGATCAGGAC GCGGGCTCAG 
GAGGAGATTC AGTCCCGCCC CTCTCACGGC ATCTCTCTGA CTCAAGGCCA CGCCTCCATG 
CAGAATAAAC CCGCCCACCA GCCGCCGGGA GCAGCACAGA ATCCAGACGC CGGCCAGAGG 
AAGAGGCTGG TGTCCACCGT CGACGACTTC ACTGACTTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315330.1
CDS	211..5235
Translation	MENSMSSDFLHVLVNLVKFNSCYLDENVSLMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYSEDAALLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGRELQVVTWDILLSIIDRLLQQIQTMGSPDLKVIVYELLSTIEELYEQNDFHGSSARFFSLVEKCADKRPDASVLTLVSYRAQAIQPAKDGWLQNLLKLMDKFFRNESRTMIRIKVLHILSFVLSTNRQLYEEELIEVVVIPQLGQIAEDRDPSVRKQATQLLVDLAEGCSTHHFSSLLDIIEKVASRPLSCSVEGDRELCVESPLEDVRTAILGLLDILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSAISGAIRLKVFDFLLLMRADSLHRLGVPNKDGALRFSPYCHCDPGESEKRVSEKKPTGSVSPPAGSPTPAAPPSSIRTAYLPYSLAFSVLLQCLKTETDWKVLKLVLDKMSCTIQYKVLILTSPCNIDNLCATLCSMVTDRIILERLKKTPDGFSLTDVQLAVVPVLTAITSYHSYLDQLRQRDLVQCLEKGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDTHEQSSFRARSTSLNERPKSLRTTKVAKQGPSANSPVKDLKDLSAMDAFRSRSISVSEHAVRRMQTSSTTCSLGSADENAVMQADDALKTVHLELTETCLDMMARYVFSNFSALPKRSPIADFLLSGGPSMTWLVGNKLVTITTSGGSRTQALLGLDMTERHGGGEMTRSDPSLHTRQTKEAPAKLESQSSQQINTNTRTRVRSISGGHALRAGPAQSLSPLVASPEGEYSGPLPPPGPPLDVLSSQRGMEEHPPPSACNPSPPLKHKHSLAEFVPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNLPLQELSSVLMAMDGVKEPPAERPTDVSQPQGKPAPIQRSNTVASLSSLCSSESSGRFHRSISWADSVVMEESGRSMASVSPAESKEVEEFEAVPSEPIFMNTSTTAPGMMSRSSSTSSQDDDKSTLEEVSEGGIPIDQPPLSLCTPGAQDPELSFTQSSSSSSTLNKSSSSPELQTLPEAFSKASSQSVDPAPTSKIPTVQACVEAPGESAGSTAGTSCSSSSVSVSSSSTSRMRLEFPPVQTGPLSPTGHRPRGHTISVSAPSSRRDRKTLERDARGGATNVEKSSGLSPSFVFLQLYHSPFFGNEANKPLLLPKSQLIDRAVKVLDQMPPYDTHKIGVVFVGAGQANNEVSILSNEYGSKRYAQFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDRGCCNKKRHIGNDFVVVVYNDSGEDYKLGTIKGQFNFVEVLIKPLDYESNLVTLQCRKDLEGLVDTSVAKIVSDRNLPLLVRQMALHANMASLVHQCRANPSDAYASKWLARLRHIKRIRTRAQEEIQSRPSHGISLTQGHASMQNKPAHQPPGAAQNPDAGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	NM_001328401.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TSC complex subunit 2 (tsc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328401.1

ORF Insert Sequence:

ATGGAGAACA GCATGTCCTC AGATTTCCTG CACGTCCTCG TCAACCTTGT CAAATTCAAC 
TCCTGCTACC TGGATGAGAA CGTTTCGCTT ATGGTCCAGA AAATCTGCCT TCTGTGCAAC 
CGGACGACAT CCTCCACAGA CATTGAGGTT GCGCTGCAGG TGCTGGACGC GGTGGTGTGT 
TATAACTGTC TGCCATCAGA CTCTCTAACA GTCTTCATCA TCACTCTGTG CCGAACTGTA 
AATGTAAAGG AGTTCTGCGA GTCCTGCTGG AAGTTGATGC GCAAAGTGTT GGGAACTCAT 
CTGGGTCACA GTGCCATATA CACCATGTGC CGAATCATGG AGGAGAGGGT GTACTCCGAG 
GACGCCGCTC TCCTCCGCGG TGCTGTGTTT TTTGTTGGGA TGGCGCTGTG GGGCGCTCAC 
AGACTCCCCG CCCTCAAAAA CACACCTACG CTGGTCCTGC CTTCCTTCTA CAAGGCCATG 
AGCTGTGCTA ATGAGGTGGT GTCCTATGAG ATCGTGCTCT CCATCACGCG CCTCATTAAA 
AAATACGGCC GCGAGCTGCA GGTGGTGACC TGGGACATCC TGCTGTCCAT CATAGACAGA 
CTCCTGCAGC AGATACAGAC GATGGGCAGT CCTGACCTGA AGGTGATCGT CTATGAGCTG 
CTGAGCACCA TCGAGGAGCT TTACGAGCAG AACGACTTCC ACGGCTCCTC GGCCCGCTTC 
TTCAGCCTGG TGGAGAAGTG TGCGGATAAA CGTCCTGATG CATCTGTGCT CACGCTGGTC 
TCCTACAGAG CTCAGGCCAT CCAGCCGGCC AAGGACGGAT GGCTGCAGAA CCTGCTCAAA 
CTCATGGACA AGTTCTTCAG GAATGAGAGC CGCACTATGA TCAGGATCAA GGTTCTGCAC 
ATCTTGTCGT TCGTCCTCAG CACAAACCGT CAGCTGTATG AGGAGGAGCT GATTGAGGTG 
GTGGTCATCC CTCAGCTGGG TCAGATAGCT GAGGATCGTG ACCCGTCAGT CAGGAAGCAG 
GCCACACAGC TGCTGGTGGA TCTGGCCGAG GGCTGCAGCA CACACCACTT CAGCAGTCTG 
CTGGACATCA TCGAGAAGGT GGCCAGTCGT CCTCTGAGCT GCTCTGTAGA GGGCGACAGA 
GAACTGTGTG TGGAGTCGCC GCTGGAGGAC GTGCGCACCG CTATACTGGG CCTGCTGGAC 
ATCCTGCAGA GCAAACTCTA TAGTCTGCCT GCCAGTCATG CCAGTCGAGT GTATGAGCTG 
CTCATCTCAC ACCTGCAGCT GCACTACAAG AACAAATACT GCAGCGCCAT CAGCGGAGCC 
ATACGCCTCA AGGTGTTTGA TTTCCTGCTG CTGATGAGGG CTGATTCTCT ACATCGTCTC 
GGGGTCCCCA ATAAAGACGG AGCTCTGAGA TTCAGCCCGT ACTGCCACTG TGACCCAGGG 
GAGTCCGAGA AGAGAGTGTC AGAGAAGAAG CCGACAGGAA GTGTGTCTCC ACCGGCAGGA 
AGTCCCACAC CGGCAGCTCC GCCCTCCTCC ATCCGCACCG CTTACCTGCC CTACAGTCTG 
GCTTTCAGTG TGCTGCTGCA GTGCCTCAAG ACGGAGACCG ACTGGAAGGT CCTGAAGCTG 
GTTCTGGATA AGATGTCCTG CACCATCCAG TATAAAGTGC TGATCCTGAC GTCTCCATGT 
AACATTGATA ATCTGTGCGC AACGCTCTGC TCGATGGTGA CGGACCGCAT TATTTTGGAG 
CGTCTGAAGA AAACCCCTGA TGGATTTTCC CTCACTGACG TTCAGCTCGC TGTGGTTCCT 
GTTCTCACGG CCATCACGTC CTACCACAGC TATCTGGATC AGCTCCGACA GAGGGACCTG 
GTGCAGTGTC TGGAGAAGGG TCTGATCTAC CGCTGTGCCA AGCAGTGTGT GGTGGCCCTG 
ACAATGTGCA CAGTGGAGAT GCCCGACATC ATGATCAAAC TACTGCCCGC CCTTATCGTC 
AAGCTCACGC ACATCTCGGC AACCGTTGCC ATGGCCTCAC CCATGCTGGA GTTCCTGTCC 
ACATTAGTGC GGCTCCCACA TCTGTATGCT AACTTCGTAG CTGAGCAGTA TGTTAGCGTG 
TTTGCCATAT CGCTGCCGTA CACCAATCCT TCAAAGTTTA ATCAGTACAT AGTGTCTCTG 
GCTCATCATG TGATCGCCAT GTGGTTCATC CGCTGCCGAC TGCCTTTCAG GAAAGATTTT 
GTTCAGTACA TCACCAAGGG TCTTCGTTCT AACGCTCTCC TGCCGTTTGA CGACACACAC 
GAGCAGAGCA GTTTCAGAGC ACGCAGCACC AGCCTGAACG AGAGGCCCAA GAGTTTGCGG 
ACGACCAAAG TGGCGAAGCA AGGCCCCAGC GCAAACTCTC CCGTTAAAGA CCTGAAAGAC 
CTTTCGGCCA TGGACGCCTT CCGCTCCCGC AGCATCAGTG TGTCCGAGCA TGCTGTGCGC 
AGGATGCAGA CGTCCAGCAC CACCTGCAGT CTGGGCTCAG CTGATGAGAA CGCTGTGATG 
CAGGCGGACG ACGCTCTGAA GACAGTCCAT CTGGAGCTCA CAGAGACCTG CCTGGACATG 
ATGGCCAGAT ACGTCTTCTC CAACTTCTCT GCTCTGCCCA AGAGGTCTCC CATCGCTGAT 
TTCCTGTTAT CTGGTGGTCC TAGTATGACG TGGCTGGTTG GGAATAAGTT AGTGACCATA 
ACCACCAGCG GCGGCTCGCG AACACAAGCT CTTCTGGGTC TGGACATGAC AGAGAGACAT 
GGAGGAGGAG AAATGACCAG GTCTGACCCG TCTCTGCACA CCCGACAGAC CAAAGAAGCT 
CCCGCTAAAC TAGAGTCTCA GTCCAGCCAA CAGATCAACA CCAACACACG CACACGAGTC 
CGCTCTATAT CAGGTGGTCA TGCTCTGCGG GCCGGGCCAG CTCAGAGTCT TAGTCCTCTG 
GTGGCGTCTC CTGAAGGCGA GTACAGCGGC CCGCTGCCTC CTCCCGGACC CCCGCTGGAC 
GTCCTGAGCT CTCAGAGGGG GATGGAGGAG CACCCGCCAC CCTCCGCCTG CAACCCATCA 
CCACCGCTCA AGCACAAGCA CAGCCTGGCA GAGTTTGTGC CCATGCTGAC GCAGGGCTGG 
GCCGAGATCT TCATCCGCAG GCCCTCAGGG AACACGAGCT GGCTGATGTG TCTGGAGAAC 
CCGCCAAGCC CGTTCTCCTC TGAGCTGGGT AATCTGCCTC TGCAGGAGCT CTCCAGCGTC 
CTCATGGCCA TGGACGGAGT GAAGGAGCCA CCGGCCGAGC GTCCCACAGA CGTGTCCCAG 
CCACAGGGCA AACCGGCTCC CATCCAGCGA TCCAACACTG TGGCCTCTCT CAGCTCTCTC 
TGCTCCTCTG AATCATCCGG CAGGTTTCAC AGGAGTATAT CCTGGGCAGA TTCAGTAGTG 
ATGGAGGAAA GTGGGCGGAG TATGGCGTCT GTTTCACCTG CTGAGTCTAA AGAGGTGGAG 
GAGTTTGAGG CCGTTCCTTC TGAACCCATC TTCATGAACA CCAGCACGAC TGCACCCGGC 
ATGATGAGCA GATCCTCCTC CACATCCAGT CAGGATGATG ATAAGTCCAC CCTGGAGGAG 
GTCAGTGAAG GAGGAATCCC CATCGACCAG CCTCCGCTCA GCCTCTGCAC CCCTGGAGCC 
CAGGACCCCG AGCTTTCCTT CACCCAAAGC AGCAGCAGCT CCAGCACCCT TAATAAATCC 
AGCTCCTCTC CTGAGCTCCA GACCCTTCCA GAGGCCTTCA GCAAAGCCTC GTCCCAGAGC 
GTGGATCCAG CTCCCACCAG CAAAATCCCT ACAGTCCAGG CCTGTGTGGA GGCTCCAGGA 
GAGAGCGCTG GATCCACCGC TGGGACATCT TGCAGCAGCT CTAGCGTGTC CGTGTCCTCC 
TCCTCCACCT CCAGAATGAG GCTGGAGTTC CCGCCAGTGC AGACAGGACC CCTGTCTCCT 
ACTGGACACC GGCCACGGGG ACACACCATA TCAGTGTCTG CTCCATCGTC CAGGAGAGAC 
CGCAAAACAC TGGAGCGGGA CGCCAGAGGA GGAGCGACCA ATGTGGAGAA GAGCTCCGGA 
CTCAGCCCCA GTTTTGTCTT CCTGCAGCTT TATCACTCGC CGTTCTTCGG CAATGAGGCC 
AACAAACCCC TGCTGCTTCC TAAATCACAG CTGATTGACC GTGCAGTGAA GGTTTTGGAT 
CAGATGCCTC CGTACGACAC TCATAAGATC GGTGTGGTGT TTGTGGGAGC CGGACAGGCC 
AATAATGAGG TGTCGATCCT GTCTAATGAG TACGGCTCGA AGCGCTATGC TCAGTTCCTG 
ACGGGTCTGG GGAAACTCAT CCACTTGAAA GACTGCGATC CCGATCAGAT CTTCCTTGGT 
GGACTGGACC AGTACGGAGA CGATGGAGAG TTCACATACT GCTGGCATGA TGACATTATG 
CAGGCTATTT TCCACATCGC CACGCTGATG CCGAACCGTG AGAGCGACCG CGGCTGCTGC 
AACAAGAAGC GGCACATCGG CAACGACTTC GTGGTGGTGG TTTATAACGA CTCGGGGGAG 
GACTACAAAC TGGGCACCAT CAAGGGTCAG TTCAACTTTG TGGAAGTGCT CATAAAGCCG 
CTGGACTACG AGAGTAACCT GGTGACACTG CAGTGCCGGA AAGATCTGGA GGGTTTGGTG 
GACACCAGTG TAGCGAAGAT CGTCTCTGAC CGGAACCTTC CCCTGCTAGT CAGACAGATG 
GCGCTTCATG CTAACATGGC GTCGCTGGTC CACCAGTGCA GGGCGAACCC ATCAGACGCA 
TACGCTTCAA AATGGCTGGC CAGACTGAGA CACATCAAGA GGATCAGGAC GCGGGCTCAG 
GAGGAGATTC AGTCCCGCCC CTCTCACGGC ATCTCTCTGA CTCAAGGCCA CGCCTCCATG 
CAGAATAAAC CCGCCCACCA GCCGCCGGGA GCAGCACAGA ATCCAGACGC CGGCCAGAGG 
AAGAGGCTGG TGTCCACCGT CGACGACTTC ACTGACTTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_021475603.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331278.1 Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X4, mRNA.

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CloneID	ODa147279
Clone ID Related Accession (Same CDS sequence)	XM_021475603.1
Accession Version	XM_021475603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AGCCCAGTAA AGAGTCGTTG AGGGACAAAT TTAAGGGCAT TTTTGGCCTC 
GGGCCCACTA GGCCGCACCA TAAACAAGCT GACAACAAAC CCTCTGAATA CATCATTACA 
CTGGACATCC TGATGGAGCT CACGTCGGAG TACAGCCTGA ACCATCGCAT CAGGATGGTT 
AATCATGTTT GTGAATTGGC GAAAACGAAG AGGTTTGAGG AGCATGCAGT GGAGGCCATC 
TGGAAGGCGG TGGAGGACCT GACGCAGCCG GAGCAGCCAC CTGAAGCCCG TCATGCGGTG 
CTGGTGCTCT TAAGGGCGAT CATACAGGGA CAGGGCGAGT GGTTGGGTCC GCTGAGAGCT 
TACTTCTTTA AGGTCATTCT GGACTATCAG CCTTCTAATG AAGAGCTGCC GGAGAGACTG 
GAGGTGTTCA AGGCCCTCAC AGAGAACGGA AAGGACATCA CATATCTAGA GGAGGAGATT 
GCTCGGTTTG TCCTGTTGTG GATGGAGAAC AGCATGTCCT CAGATTTCCT GCACGTCCTC 
GTCAACCTTG TCAAATTCAA CTCCTGCTAC CTGGATGAGA ACGTTTCGCT TATGGTCCAG 
AAAATCTGCC TTCTGTGCAA CCGGACGACA TCCTCCACAG ACATTGAGGT TGCGCTGCAG 
GTGCTGGACG CGGTGGTGTG TTATAACTGT CTGCCATCAG ACTCTCTAAC AGTCTTCATC 
ATCACTCTGT GCCGAACTGT AAATGTAAAG GAGTTCTGCG AGTCCTGCTG GAAGTTGATG 
CGCAAAGTGT TGGGAACTCA TCTGGGTCAC AGTGCCATAT ACACCATGTG CCGAATCATG 
GAGGAGAGGG TGTACTCCGA GGACGCCGCT CTCCTCCGCG GTGCTGTGTT TTTTGTTGGG 
ATGGCGCTGT GGGGCGCTCA CAGACTCCCC GCCCTCAAAA ACACACCTAC GCTGGTCCTG 
CCTTCCTTCT ACAAGGCCAT GAGCTGTGCT AATGAGGTGG TGTCCTATGA GATCGTGCTC 
TCCATCACGC GCCTCATTAA AAAATACGGC CGCGAGCTGC AGGTGGTGAC CTGGGACATC 
CTGCTGTCCA TCATAGACAG ACTCCTGCAG CAGATACAGA CGATGGGCAG TCCTGACCTG 
AAGGTGATCG TCTATGAGCT GCTGAGCACC ATCGAGGAGC TTTACGAGCA GAACGACTTC 
CACGGCTCCT CGGCCCGCTT CTTCAGCCTG GTGGAGAAGT GTGCGGATAA ACGTCCTGAT 
GCATCTGTGC TCACGCTGGT CTCCTACAGA GCTCAGGCCA TCCAGCCGGC CAAGGACGGA 
TGGCTGCAGA ACCTGCTCAA ACTCATGGAC AAGTTCTTCA GGAATGAGAG CCGCACTATG 
ATCAGGATCA AGGTTCTGCA CATCTTGTCG TTCGTCCTCA GCACAAACCG TCAGCTGTAT 
GAGGAGGAGC TGATTGAGGT GGTGGTCATC CCTCAGCTGG GTCAGATAGC TGAGGATCGT 
GACCCGTCAG TCAGGAAGCA GGCCACACAG CTGCTGGTGG ATCTGGCCGA GGGCTGCAGC 
ACACACCACT TCAGCAGTCT GCTGGACATC ATCGAGAAGG TGGCCAGTCG TCCTCTGAGC 
TGCTCTGTAG AGGGCGACAG AGAACTGTGT GTGGAGTCGC CGCTGGAGGA CGTGCGCACC 
GCTATACTGG GCCTGCTGGA CATCCTGCAG AGCAAACTCT ATAGTCTGCC TGCCAGTCAT 
GCCAGTCGAG TGTATGAGCT GCTCATCTCA CACCTGCAGC TGCACTACAA GAACAAATAC 
TGCAGCGCCA TCAGCGGAGC CATACGCCTC AAGGTGTTTG ATTTCCTGCT GCTGATGAGG 
GCTGATTCTC TACATCGTCT CGGGGTCCCC AATAAAGACG GAGCTCTGAG ATTCAGCCCG 
TACTGCCACT GTGACCCAGG GGAGTCCGAG AAGAGAGTGT CAGAGAAGAA GCCGACAGGA 
AGTGTGTCTC CACCGGCAGG AAGTCCCACA CCGGCAGCTC CGCCCTCCTC CATCCGCACC 
GCTTACCTGC CCTACAGTCT GGCTTTCAGT GTGCTGCTGC AGTGCCTCAA GACGGAGACC 
GACTGGAAGG TCCTGAAGCT GGTTCTGGAT AAGATGTCCT GCACCATCCA GTATAAAGTG 
CTGATCCTGA CGTCTCCATG TAACATTGAT AATCTGTGCG CAACGCTCTG CTCGATGGTG 
ACGGACCGCA TTATTTTGGA GCGTCTGAAG AAAACCCCTG ATGGATTTTC CCTCACTGAC 
GTTCAGCTCG CTGTGGTTCC TGTTCTCACG GCCATCACGT CCTACCACAG CTATCTGGAT 
CAGCTCCGAC AGAGGGACCT GGTGCAGTGT CTGGAGAAGG GTCTGATCTA CCGCTGTGCC 
AAGCAGTGTG TGGTGGCCCT GACAATGTGC ACAGTGGAGA TGCCCGACAT CATGATCAAA 
CTACTGCCCG CCCTTATCGT CAAGCTCACG CACATCTCGG CAACCGTTGC CATGGCCTCA 
CCCATGCTGG AGTTCCTGTC CACATTAGTG CGGCTCCCAC ATCTGTATGC TAACTTCGTA 
GCTGAGCAGT ATGTTAGCGT GTTTGCCATA TCGCTGCCGT ACACCAATCC TTCAAAGTTT 
AATCAGTACA TAGTGTCTCT GGCTCATCAT GTGATCGCCA TGTGGTTCAT CCGCTGCCGA 
CTGCCTTTCA GGAAAGATTT TGTTCAGTAC ATCACCAAGG GTCTTCGTTC TAACGCTCTC 
CTGCCGTTTG ACGACACACA CGAGCAGAGC AGTTTCAGAG CACGCAGCAC CAGCCTGAAC 
GAGAGGCCCA AGAGTTTGCG GACGACCAAA GTGGCGAAGC AAGGCCCCAG CGCAAACTCT 
CCCGTTAAAG ACCTGAAAGA CCTTTCGGCC ATGGACGCCT TCCGCTCCCG CAGCATCAGT 
GTGTCCGAGC ATGCTGTGCG CAGGATGCAG ACGTCCAGCA CCACCTGCAG TCTGGGCTCA 
GCTGATGAGA ACGCTGTGAT GCAGGCGGAC GACGCTCTGA AGACAGTCCA TCTGGAGCTC 
ACAGAGACCT GCCTGGACAT GATGGCCAGA TACGTCTTCT CCAACTTCTC TGCTCTGCCC 
AAGAGGTCTC CCATCGCTGA TTTCCTGTTA TCTGGTGGTC CTAGTATGAC GTGGCTGGTT 
GGGAATAAGT TAGTGACCAT AACCACCAGC GGCGGCTCGC GAACACAAGC TCTTCTGGGT 
CTGGACATGA CAGAGAGACA TGGAGGAGGA GAAATGACCA GGTCTGACCC GTCTCTGCAC 
ACCCGACAGA CCAAAGAAGC TCCCGCTAAA CTAGAGTCTC AGTCCAGCCA ACAGATCAAC 
ACCAACACAC GCACACGAGT CCGCTCTATA TCAGGTGGTC ATGCTCTGCG GGCCGGGCCA 
GCTCAGAGTC TTAGTCCTCT GGTGGCGTCT CCTGAAGGCG AGTACAGCGG CCCGCTGCCT 
CCTCCCGGAC CCCCGCTGGA CGTCCTGAGC TCTCAGAGGG GGATGGAGGA GCACCCGCCA 
CCCTCCGCCT GCAACCCATC ACCACCGCTC AAGCACAAGC ACAGCCTGGC AGAGTTTGTG 
CCCATGCTGA CGCAGGGCTG GGCCGAGATC TTCATCCGCA GGCCCTCAGG GAACACGAGC 
TGGCTGATGT GTCTGGAGAA CCCGCCAAGC CCGTTCTCCT CTGAGCTGGG TAATCTGCCT 
CTGCAGGAGC TCTCCAGCGT CCTCATGGCC ATGGACGGAG TGAAGGAGCC ACCGGCCGAG 
CGTCCCACAG ACGTGTCCCA GCCACAGGGC AAACCGGCTC CCATCCAGCG ATCCAACACT 
GTGGCCTCTC TCAGCTCTCT CTGCTCCTCT GAATCATCCG GCAGGTTTCA CAGGAGTATA 
TCCTGGGCAG ATTCAGTAGT GATGGAGGAA AGTGGGCGGA GTATGGCGTC TGTTTCACCT 
GCTGAGTCTA AAGAGGTGGA GGAGTTTGAG GCCGTTCCTT CTGAACCCAT CTTCATGAAC 
ACCAGCACGA CTGCACCCGG CATGATGAGC AGATCCTCCT CCACATCCAG TCAGGATGAT 
GATAAGTCCA CCCTGGAGGA GGTCAGTGAA GGAGGAATCC CCATCGACCA GCCTCCGCTC 
AGCCTCTGCA CCCCTGGAGC CCAGGACCCC GAGCTTTCCT TCACCCAAAG CAGCAGCAGC 
TCCAGCACCC TTAATAAATC CAGCTCCTCT CCTGAGCTCC AGACCCTTCC AGAGGCCTTC 
AGCAAAGCCT CGTCCCAGAG CGTGGATCCA GCTCCCACCA GCAAAATCCC TACAGTCCAG 
GCCTGTGTGG AGGCTCCAGG AGAGAGCGCT GGATCCACCG CTGGGACATC TTGCAGCAGC 
TCTAGCGTGT CCGTGTCCTC CTCCTCCACC TCCAGAATGA GGCTGGAGTT CCCGCCAGTG 
CAGACAGGAC CCCTGTCTCC TACTGGACAC CGGCCACGGG GACACACCAT ATCAGTGTCT 
GCTCCATCGT CCAGGAGAGA CCGCAAAACA CTGGAGCGGG ACGCCAGAGG AGGAGCGACC 
AATGTGGAGA AGAGCTCCGG ACTCAGCCCC AGTTTTGTCT TCCTGCAGCT TTATCACTCG 
CCGTTCTTCG GCAATGAGGC CAACAAACCC CTGCTGCTTC CTAAATCACA GCTGATTGAC 
CGTGCAGTGA AGGTTTTGGA TCAGATGCCT CCGTACGACA CTCATAAGAT CGGTGTGGTG 
TTTGTGGGAG CCGGACAGGC CAATAATGAG GTGTCGATCC TGTCTAATGA GTACGGCTCG 
AAGCGCTATG CTCAGTTCCT GACGGGTCTG GGGAAACTCA TCCACTTGAA AGACTGCGAT 
CCCGATCAGA TCTTCCTTGG TGGACTGGAC CAGTACGGAG ACGATGGAGA GTTCACATAC 
TGCTGGCATG ATGACATTAT GCAGGCTATT TTCCACATCG CCACGCTGAT GCCGAACCGT 
GAGAGCGACC GCGGCTGCTG CAACAAGAAG CGGCACATCG GCAACGACTT CGTGGTGGTG 
GTTTATAACG ACTCGGGGGA GGACTACAAA CTGGGCACCA TCAAGGGTCA GTTCAACTTT 
GTGGAAGTGC TCATAAAGCC GCTGGACTAC GAGAGTAACC TGGTGACACT GCAGTGCCGG 
AAAGATCTGG AGGGTTTGGT GGACACCAGT GTAGCGAAGA TCGTCTCTGA CCGGAACCTT 
CCCCTGCTAG TCAGACAGAT GGCGCTTCAT GCTAACATGG CGTCGCTGGT CCACCAGTGC 
AGGGCGAACC CATCAGACGC ATACGCTTCA AAATGGCTGG CCAGACTGAG ACACATCAAG 
AGGATCAGGA CGCGGGCTCA GGAGGAGATT CAGTCCCGCC CCTCTCACGG CATCTCTCTG 
ACTCAAGGCC ACGCCTCCAT GCAGAATAAA CCCGCCCACC AGCCGCCGGG AGCAGCACAG 
AATCCAGACG CCGGCCAGAG GAAGAGGCTG GTGTCCACCG TCGACGACTT CACTGACTTT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331278.1
CDS	187..5712
Translation	MNKQPSKESLRDKFKGIFGLGPTRPHHKQADNKPSEYIITLDILMELTSEYSLNHRIRMVNHVCELAKTKRFEEHAVEAIWKAVEDLTQPEQPPEARHAVLVLLRAIIQGQGEWLGPLRAYFFKVILDYQPSNEELPERLEVFKALTENGKDITYLEEEIARFVLLWMENSMSSDFLHVLVNLVKFNSCYLDENVSLMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYSEDAALLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGRELQVVTWDILLSIIDRLLQQIQTMGSPDLKVIVYELLSTIEELYEQNDFHGSSARFFSLVEKCADKRPDASVLTLVSYRAQAIQPAKDGWLQNLLKLMDKFFRNESRTMIRIKVLHILSFVLSTNRQLYEEELIEVVVIPQLGQIAEDRDPSVRKQATQLLVDLAEGCSTHHFSSLLDIIEKVASRPLSCSVEGDRELCVESPLEDVRTAILGLLDILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSAISGAIRLKVFDFLLLMRADSLHRLGVPNKDGALRFSPYCHCDPGESEKRVSEKKPTGSVSPPAGSPTPAAPPSSIRTAYLPYSLAFSVLLQCLKTETDWKVLKLVLDKMSCTIQYKVLILTSPCNIDNLCATLCSMVTDRIILERLKKTPDGFSLTDVQLAVVPVLTAITSYHSYLDQLRQRDLVQCLEKGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDTHEQSSFRARSTSLNERPKSLRTTKVAKQGPSANSPVKDLKDLSAMDAFRSRSISVSEHAVRRMQTSSTTCSLGSADENAVMQADDALKTVHLELTETCLDMMARYVFSNFSALPKRSPIADFLLSGGPSMTWLVGNKLVTITTSGGSRTQALLGLDMTERHGGGEMTRSDPSLHTRQTKEAPAKLESQSSQQINTNTRTRVRSISGGHALRAGPAQSLSPLVASPEGEYSGPLPPPGPPLDVLSSQRGMEEHPPPSACNPSPPLKHKHSLAEFVPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNLPLQELSSVLMAMDGVKEPPAERPTDVSQPQGKPAPIQRSNTVASLSSLCSSESSGRFHRSISWADSVVMEESGRSMASVSPAESKEVEEFEAVPSEPIFMNTSTTAPGMMSRSSSTSSQDDDKSTLEEVSEGGIPIDQPPLSLCTPGAQDPELSFTQSSSSSSTLNKSSSSPELQTLPEAFSKASSQSVDPAPTSKIPTVQACVEAPGESAGSTAGTSCSSSSVSVSSSSTSRMRLEFPPVQTGPLSPTGHRPRGHTISVSAPSSRRDRKTLERDARGGATNVEKSSGLSPSFVFLQLYHSPFFGNEANKPLLLPKSQLIDRAVKVLDQMPPYDTHKIGVVFVGAGQANNEVSILSNEYGSKRYAQFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDRGCCNKKRHIGNDFVVVVYNDSGEDYKLGTIKGQFNFVEVLIKPLDYESNLVTLQCRKDLEGLVDTSVAKIVSDRNLPLLVRQMALHANMASLVHQCRANPSDAYASKWLARLRHIKRIRTRAQEEIQSRPSHGISLTQGHASMQNKPAHQPPGAAQNPDAGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_021475603.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475603.1

ORF Insert Sequence:

ATGAATAAAC AGCCCAGTAA AGAGTCGTTG AGGGACAAAT TTAAGGGCAT TTTTGGCCTC 
GGGCCCACTA GGCCGCACCA TAAACAAGCT GACAACAAAC CCTCTGAATA CATCATTACA 
CTGGACATCC TGATGGAGCT CACGTCGGAG TACAGCCTGA ACCATCGCAT CAGGATGGTT 
AATCATGTTT GTGAATTGGC GAAAACGAAG AGGTTTGAGG AGCATGCAGT GGAGGCCATC 
TGGAAGGCGG TGGAGGACCT GACGCAGCCG GAGCAGCCAC CTGAAGCCCG TCATGCGGTG 
CTGGTGCTCT TAAGGGCGAT CATACAGGGA CAGGGCGAGT GGTTGGGTCC GCTGAGAGCT 
TACTTCTTTA AGGTCATTCT GGACTATCAG CCTTCTAATG AAGAGCTGCC GGAGAGACTG 
GAGGTGTTCA AGGCCCTCAC AGAGAACGGA AAGGACATCA CATATCTAGA GGAGGAGATT 
GCTCGGTTTG TCCTGTTGTG GATGGAGAAC AGCATGTCCT CAGATTTCCT GCACGTCCTC 
GTCAACCTTG TCAAATTCAA CTCCTGCTAC CTGGATGAGA ACGTTTCGCT TATGGTCCAG 
AAAATCTGCC TTCTGTGCAA CCGGACGACA TCCTCCACAG ACATTGAGGT TGCGCTGCAG 
GTGCTGGACG CGGTGGTGTG TTATAACTGT CTGCCATCAG ACTCTCTAAC AGTCTTCATC 
ATCACTCTGT GCCGAACTGT AAATGTAAAG GAGTTCTGCG AGTCCTGCTG GAAGTTGATG 
CGCAAAGTGT TGGGAACTCA TCTGGGTCAC AGTGCCATAT ACACCATGTG CCGAATCATG 
GAGGAGAGGG TGTACTCCGA GGACGCCGCT CTCCTCCGCG GTGCTGTGTT TTTTGTTGGG 
ATGGCGCTGT GGGGCGCTCA CAGACTCCCC GCCCTCAAAA ACACACCTAC GCTGGTCCTG 
CCTTCCTTCT ACAAGGCCAT GAGCTGTGCT AATGAGGTGG TGTCCTATGA GATCGTGCTC 
TCCATCACGC GCCTCATTAA AAAATACGGC CGCGAGCTGC AGGTGGTGAC CTGGGACATC 
CTGCTGTCCA TCATAGACAG ACTCCTGCAG CAGATACAGA CGATGGGCAG TCCTGACCTG 
AAGGTGATCG TCTATGAGCT GCTGAGCACC ATCGAGGAGC TTTACGAGCA GAACGACTTC 
CACGGCTCCT CGGCCCGCTT CTTCAGCCTG GTGGAGAAGT GTGCGGATAA ACGTCCTGAT 
GCATCTGTGC TCACGCTGGT CTCCTACAGA GCTCAGGCCA TCCAGCCGGC CAAGGACGGA 
TGGCTGCAGA ACCTGCTCAA ACTCATGGAC AAGTTCTTCA GGAATGAGAG CCGCACTATG 
ATCAGGATCA AGGTTCTGCA CATCTTGTCG TTCGTCCTCA GCACAAACCG TCAGCTGTAT 
GAGGAGGAGC TGATTGAGGT GGTGGTCATC CCTCAGCTGG GTCAGATAGC TGAGGATCGT 
GACCCGTCAG TCAGGAAGCA GGCCACACAG CTGCTGGTGG ATCTGGCCGA GGGCTGCAGC 
ACACACCACT TCAGCAGTCT GCTGGACATC ATCGAGAAGG TGGCCAGTCG TCCTCTGAGC 
TGCTCTGTAG AGGGCGACAG AGAACTGTGT GTGGAGTCGC CGCTGGAGGA CGTGCGCACC 
GCTATACTGG GCCTGCTGGA CATCCTGCAG AGCAAACTCT ATAGTCTGCC TGCCAGTCAT 
GCCAGTCGAG TGTATGAGCT GCTCATCTCA CACCTGCAGC TGCACTACAA GAACAAATAC 
TGCAGCGCCA TCAGCGGAGC CATACGCCTC AAGGTGTTTG ATTTCCTGCT GCTGATGAGG 
GCTGATTCTC TACATCGTCT CGGGGTCCCC AATAAAGACG GAGCTCTGAG ATTCAGCCCG 
TACTGCCACT GTGACCCAGG GGAGTCCGAG AAGAGAGTGT CAGAGAAGAA GCCGACAGGA 
AGTGTGTCTC CACCGGCAGG AAGTCCCACA CCGGCAGCTC CGCCCTCCTC CATCCGCACC 
GCTTACCTGC CCTACAGTCT GGCTTTCAGT GTGCTGCTGC AGTGCCTCAA GACGGAGACC 
GACTGGAAGG TCCTGAAGCT GGTTCTGGAT AAGATGTCCT GCACCATCCA GTATAAAGTG 
CTGATCCTGA CGTCTCCATG TAACATTGAT AATCTGTGCG CAACGCTCTG CTCGATGGTG 
ACGGACCGCA TTATTTTGGA GCGTCTGAAG AAAACCCCTG ATGGATTTTC CCTCACTGAC 
GTTCAGCTCG CTGTGGTTCC TGTTCTCACG GCCATCACGT CCTACCACAG CTATCTGGAT 
CAGCTCCGAC AGAGGGACCT GGTGCAGTGT CTGGAGAAGG GTCTGATCTA CCGCTGTGCC 
AAGCAGTGTG TGGTGGCCCT GACAATGTGC ACAGTGGAGA TGCCCGACAT CATGATCAAA 
CTACTGCCCG CCCTTATCGT CAAGCTCACG CACATCTCGG CAACCGTTGC CATGGCCTCA 
CCCATGCTGG AGTTCCTGTC CACATTAGTG CGGCTCCCAC ATCTGTATGC TAACTTCGTA 
GCTGAGCAGT ATGTTAGCGT GTTTGCCATA TCGCTGCCGT ACACCAATCC TTCAAAGTTT 
AATCAGTACA TAGTGTCTCT GGCTCATCAT GTGATCGCCA TGTGGTTCAT CCGCTGCCGA 
CTGCCTTTCA GGAAAGATTT TGTTCAGTAC ATCACCAAGG GTCTTCGTTC TAACGCTCTC 
CTGCCGTTTG ACGACACACA CGAGCAGAGC AGTTTCAGAG CACGCAGCAC CAGCCTGAAC 
GAGAGGCCCA AGAGTTTGCG GACGACCAAA GTGGCGAAGC AAGGCCCCAG CGCAAACTCT 
CCCGTTAAAG ACCTGAAAGA CCTTTCGGCC ATGGACGCCT TCCGCTCCCG CAGCATCAGT 
GTGTCCGAGC ATGCTGTGCG CAGGATGCAG ACGTCCAGCA CCACCTGCAG TCTGGGCTCA 
GCTGATGAGA ACGCTGTGAT GCAGGCGGAC GACGCTCTGA AGACAGTCCA TCTGGAGCTC 
ACAGAGACCT GCCTGGACAT GATGGCCAGA TACGTCTTCT CCAACTTCTC TGCTCTGCCC 
AAGAGGTCTC CCATCGCTGA TTTCCTGTTA TCTGGTGGTC CTAGTATGAC GTGGCTGGTT 
GGGAATAAGT TAGTGACCAT AACCACCAGC GGCGGCTCGC GAACACAAGC TCTTCTGGGT 
CTGGACATGA CAGAGAGACA TGGAGGAGGA GAAATGACCA GGTCTGACCC GTCTCTGCAC 
ACCCGACAGA CCAAAGAAGC TCCCGCTAAA CTAGAGTCTC AGTCCAGCCA ACAGATCAAC 
ACCAACACAC GCACACGAGT CCGCTCTATA TCAGGTGGTC ATGCTCTGCG GGCCGGGCCA 
GCTCAGAGTC TTAGTCCTCT GGTGGCGTCT CCTGAAGGCG AGTACAGCGG CCCGCTGCCT 
CCTCCCGGAC CCCCGCTGGA CGTCCTGAGC TCTCAGAGGG GGATGGAGGA GCACCCGCCA 
CCCTCCGCCT GCAACCCATC ACCACCGCTC AAGCACAAGC ACAGCCTGGC AGAGTTTGTG 
CCCATGCTGA CGCAGGGCTG GGCCGAGATC TTCATCCGCA GGCCCTCAGG GAACACGAGC 
TGGCTGATGT GTCTGGAGAA CCCGCCAAGC CCGTTCTCCT CTGAGCTGGG TAATCTGCCT 
CTGCAGGAGC TCTCCAGCGT CCTCATGGCC ATGGACGGAG TGAAGGAGCC ACCGGCCGAG 
CGTCCCACAG ACGTGTCCCA GCCACAGGGC AAACCGGCTC CCATCCAGCG ATCCAACACT 
GTGGCCTCTC TCAGCTCTCT CTGCTCCTCT GAATCATCCG GCAGGTTTCA CAGGAGTATA 
TCCTGGGCAG ATTCAGTAGT GATGGAGGAA AGTGGGCGGA GTATGGCGTC TGTTTCACCT 
GCTGAGTCTA AAGAGGTGGA GGAGTTTGAG GCCGTTCCTT CTGAACCCAT CTTCATGAAC 
ACCAGCACGA CTGCACCCGG CATGATGAGC AGATCCTCCT CCACATCCAG TCAGGATGAT 
GATAAGTCCA CCCTGGAGGA GGTCAGTGAA GGAGGAATCC CCATCGACCA GCCTCCGCTC 
AGCCTCTGCA CCCCTGGAGC CCAGGACCCC GAGCTTTCCT TCACCCAAAG CAGCAGCAGC 
TCCAGCACCC TTAATAAATC CAGCTCCTCT CCTGAGCTCC AGACCCTTCC AGAGGCCTTC 
AGCAAAGCCT CGTCCCAGAG CGTGGATCCA GCTCCCACCA GCAAAATCCC TACAGTCCAG 
GCCTGTGTGG AGGCTCCAGG AGAGAGCGCT GGATCCACCG CTGGGACATC TTGCAGCAGC 
TCTAGCGTGT CCGTGTCCTC CTCCTCCACC TCCAGAATGA GGCTGGAGTT CCCGCCAGTG 
CAGACAGGAC CCCTGTCTCC TACTGGACAC CGGCCACGGG GACACACCAT ATCAGTGTCT 
GCTCCATCGT CCAGGAGAGA CCGCAAAACA CTGGAGCGGG ACGCCAGAGG AGGAGCGACC 
AATGTGGAGA AGAGCTCCGG ACTCAGCCCC AGTTTTGTCT TCCTGCAGCT TTATCACTCG 
CCGTTCTTCG GCAATGAGGC CAACAAACCC CTGCTGCTTC CTAAATCACA GCTGATTGAC 
CGTGCAGTGA AGGTTTTGGA TCAGATGCCT CCGTACGACA CTCATAAGAT CGGTGTGGTG 
TTTGTGGGAG CCGGACAGGC CAATAATGAG GTGTCGATCC TGTCTAATGA GTACGGCTCG 
AAGCGCTATG CTCAGTTCCT GACGGGTCTG GGGAAACTCA TCCACTTGAA AGACTGCGAT 
CCCGATCAGA TCTTCCTTGG TGGACTGGAC CAGTACGGAG ACGATGGAGA GTTCACATAC 
TGCTGGCATG ATGACATTAT GCAGGCTATT TTCCACATCG CCACGCTGAT GCCGAACCGT 
GAGAGCGACC GCGGCTGCTG CAACAAGAAG CGGCACATCG GCAACGACTT CGTGGTGGTG 
GTTTATAACG ACTCGGGGGA GGACTACAAA CTGGGCACCA TCAAGGGTCA GTTCAACTTT 
GTGGAAGTGC TCATAAAGCC GCTGGACTAC GAGAGTAACC TGGTGACACT GCAGTGCCGG 
AAAGATCTGG AGGGTTTGGT GGACACCAGT GTAGCGAAGA TCGTCTCTGA CCGGAACCTT 
CCCCTGCTAG TCAGACAGAT GGCGCTTCAT GCTAACATGG CGTCGCTGGT CCACCAGTGC 
AGGGCGAACC CATCAGACGC ATACGCTTCA AAATGGCTGG CCAGACTGAG ACACATCAAG 
AGGATCAGGA CGCGGGCTCA GGAGGAGATT CAGTCCCGCC CCTCTCACGG CATCTCTCTG 
ACTCAAGGCC ACGCCTCCAT GCAGAATAAA CCCGCCCACC AGCCGCCGGG AGCAGCACAG 
AATCCAGACG CCGGCCAGAG GAAGAGGCTG GTGTCCACCG TCGACGACTT CACTGACTTT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_009294973.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293248.2 Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.

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CloneID	ODa53725
Clone ID Related Accession (Same CDS sequence)	XM_009294973.2
Accession Version	XM_009294973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tuberin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) and transcript sequence (GDQH01012854.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 520 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAATAAAC AGCCCAGTAA AGAGTCGTTG AGGGACAAAT TTAAGGGCAT TTTTGGCCTC 
GGGCCCACTA GGCCGCACCA TAAACAAGCT GACAACAAAC CCTCTGAATA CATCATTACA 
CTGGACATCC TGATGGAGCT CACGTCGGAG TACAGCCTGA ACCATCGCAT CAGGATGGTT 
AATCATGTTT GTGAATTGGC GAAAACGAAG AGGTTTGAGG AGCATGCAGT GGAGGCCATC 
TGGAAGGCGG TGGAGGACCT GACGCAGCCG GAGCAGCCAC CTGAAGCCCG TCATGCGGTG 
CTGGTGCTCT TAAGGGCGAT CATACAGGGA CAGGGCGAGT GGTTGGGTCC GCTGAGAGCT 
TACTTCTTTA AGGTCATTCT GGACTATCAG CCTTCTAATG AAGAGCTGCC GGAGAGACTG 
GAGGTGTTCA AGGCCCTCAC AGAGAACGGA AAGGACATCA CATATCTAGA GGAGGAGATT 
GCTCGGTTTG TCCTGTTGTG GATGGAGAAC AGCATGTCCT CAGATTTCCT GCACGTCCTC 
GTCAACCTTG TCAAATTCAA CTCCTGCTAC CTGGATGAGA ACGTTTCGCT TATGGTCCAG 
AAAATCTGCC TTCTGTGCAA CCGGACGACA TCCTCCACAG ACATTGAGGT TGCGCTGCAG 
GTGCTGGACG CGGTGGTGTG TTATAACTGT CTGCCATCAG ACTCTCTAAC AGTCTTCATC 
ATCACTCTGT GCCGAACTGT AAATGTAAAG GAGTTCTGCG AGTCCTGCTG GAAGTTGATG 
CGCAAAGTGT TGGGAACTCA TCTGGGTCAC AGTGCCATAT ACACCATGTG CCGAATCATG 
GAGGAGAGGG TGTACTCCGA GGACGCCGCT CTCCTCCGCG GTGCTGTGTT TTTTGTTGGG 
ATGGCGCTGT GGGGCGCTCA CAGACTCCCC GCCCTCAAAA ACACACCTAC GCTGGTCCTG 
CCTTCCTTCT ACAAGGCCAT GAGCTGTGCT AATGAGGTGG TGTCCTATGA GATCGTGCTC 
TCCATCACGC GCCTCATTAA AAAATACGGC CGCGAGCTGC AGGTGGTGAC CTGGGACATC 
CTGCTGTCCA TCATAGACAG ACTCCTGCAG CAGATACAGA CGATGGGCAG TCCTGACCTG 
AAGGTGATCG TCTATGAGCT GCTGAGCACC ATCGAGGAGC TTTACGAGCA GAACGACTTC 
CACGGCTCCT CGGCCCGCTT CTTCAGCCTG GTGGAGAAGT GTGCGGATAA ACGTCCTGAT 
GCATCTGTGC TCACGCTGGT CTCCTACAGA GCTCAGGCCA TCCAGCCGGC CAAGGACGGA 
TGGCTGCAGA ACCTGCTCAA ACTCATGGAC AAGTTCTTCA GGAATGAGAG CCGCACTATG 
ATCAGGATCA AGGTTCTGCA CATCTTGTCG TTCGTCCTCA GCACAAACCG TCAGCTGTAT 
GAGGAGGAGC TGATTGAGGT GGTGGTCATC CCTCAGCTGG GTCAGATAGC TGAGGATCGT 
GACCCGTCAG TCAGGAAGCA GGCCACACAG CTGCTGGTGG ATCTGGCCGA GGGCTGCAGC 
ACACACCACT TCAGCAGTCT GCTGGACATC ATCGAGAAGG TGGCCAGTCG TCCTCTGAGC 
TGCTCTGTAG AGGGCGACAG AGAACTGTGT GTGGAGTCGC CGCTGGAGGA CGTGCGCACC 
GCTATACTGG GCCTGCTGGA CATCCTGCAG AGCAAACTCT ATAGTCTGCC TGCCAGTCAT 
GCCAGTCGAG TGTATGAGCT GCTCATCTCA CACCTGCAGC TGCACTACAA GAACAAATAC 
TGCAGCGCCA TCAGCGGAGC CATACGCCTC AAGGTGTTTG ATTTCCTGCT GCTGATGAGG 
GCTGATTCTC TACATCGTCT CGGGGTCCCC AATAAAGACG GAGCTCTGAG ATTCAGCCCG 
TACTGCCACT GTGACCCAGG GGAGTCCGAG AAGAGAGTGT CAGAGAAGAA GCCGACAGGA 
AGTGTGTCTC CACCGGCAGG AAGTCCCACA CCGGCAGCTC CGCCCTCCTC CATCCGCACC 
GCTTACCTGC CCTACAGTCT GGCTTTCAGT GTGCTGCTGC AGTGCCTCAA GACGGAGACC 
GACTGGAAGG TCCTGAAGCT GGTTCTGGAT AAGATGTCCT GCACCATCCA GTATAAAGTG 
CTGATCCTGA CGTCTCCATG TAACATTGAT AATCTGTGCG CAACGCTCTG CTCGATGGTG 
ACGGACCGCA TTATTTTGGA GCGTCTGAAG AAAACCCCTG ATGGATTTTC CCTCACTGAC 
GTTCAGCTCG CTGTGGTTCC TGTTCTCACG GCCATCACGT CCTACCACAG CTATCTGGAT 
CAGCTCCGAC AGAGGGACCT GGTGCAGTGT CTGGAGAAGG GTCTGATCTA CCGCTGTGCC 
AAGCAGTGTG TGGTGGCCCT GACAATGTGC ACAGTGGAGA TGCCCGACAT CATGATCAAA 
CTACTGCCCG CCCTTATCGT CAAGCTCACG CACATCTCGG CAACCGTTGC CATGGCCTCA 
CCCATGCTGG AGTTCCTGTC CACATTAGTG CGGCTCCCAC ATCTGTATGC TAACTTCGTA 
GCTGAGCAGT ATGTTAGCGT GTTTGCCATA TCGCTGCCGT ACACCAATCC TTCAAAGTTT 
AATCAGTACA TAGTGTCTCT GGCTCATCAT GTGATCGCCA TGTGGTTCAT CCGCTGCCGA 
CTGCCTTTCA GGAAAGATTT TGTTCAGTAC ATCACCAAGG GTCTTCGTTC TAACGCTCTC 
CTGCCGTTTG ACGACACACA CGAGCAGAGC AGTTTCAGAG CACGCAGCAC CAGCCTGAAC 
GAGAGGCCCA AGAGTTTGCG GACGACCAAA GTGGCGAAGC AAGGCCCCAG CGCAAACTCT 
CCCGTTAAAG ACCTGAAAGA CCTTTCGGCC ATGGACGCCT TCCGCTCCCG CAGCATCAGT 
GTGTCCGAGC ATGCTGTGCG CAGGATGCAG ACGTCCAGCA CCACCTGCAG TCTGGGCTCA 
GCTGATGAGA ACGCTGTGAT GCAGGCGGAC GACGCTCTGA AGACAGTCCA TCTGGAGCTC 
ACAGAGACCT GCCTGGACAT GATGGCCAGA TACGTCTTCT CCAACTTCTC TGCTCTGCCC 
AAGAGGTCTC CCATCGCTGA TTTCCTGTTA TCTGGTGGTC CTAGTATGAC GTGGCTGGTT 
GGGAATAAGT TAGTGACCAT AACCACCAGC GGCGGCTCGC GAACACAAGC TCTTCTGGGT 
CTGGACATGA CAGAGAGACA TGGAGGAGGA GAAATGACCA GGTCTGACCC GTCTCTGCAC 
ACCCGACAGA CCAAAGAAGC TCCCGCTAAA CTAGAGTCTC AGTCCAGCCA ACAGATCAAC 
ACCAACACAC GCACACGAGT CCGCTCTATA TCAGGTGGTC ATGCTCTGCG GGCCGGGCCA 
GCTCAGAGTC TTAGTCCTCT GGTGGCGTCT CCTGAAGGCG AGTACAGCGG CCCGCTGCCT 
CCTCCCGGAC CCCCGCTGGA CGTCCTGAGC TCTCAGAGGG GGATGGAGGA GCACCCGCCA 
CCCTCCGCCT GCAACCCATC ACCACCGCTC AAGCACAAGC ACAGCCTGGC AGAGTTTGTG 
CCCATGCTGA CGCAGGGCTG GGCCGAGATC TTCATCCGCA GGCCCTCAGG GAACACGAGC 
TGGCTGATGT GTCTGGAGAA CCCGCCAAGC CCGTTCTCCT CTGAGCTGGG TAATCTGCCT 
CTGCAGGAGC TCTCCAGCGT CCTCATGGCC ATGGACGGAG TGAAGGAGCC ACCGGCCGAG 
CGTCCCACAG ACGTGTCCCA GCCACAGGGC AAACCGGCTC CCATCCAGCG ATCCAACACT 
GATTCAGTAG TGATGGAGGA AAGTGGGCGG AGTATGGCGT CTGTTTCACC TGCTGAGTCT 
AAAGAGGTGG AGGAGTTTGA GGCCGTTCCT TCTGAACCCA TCTTCATGAA CACCAGCACG 
ACTGCACCCG GCATGATGAG CAGATCCTCC TCCACATCCA GTCAGGATGA TGATAAGTCC 
ACCCTGGAGG AGGTCAGTGA AGGAGGAATC CCCATCGACC AGCCTCCGCT CAGCCTCTGC 
ACCCCTGGAG CCCAGGACCC CGAGCTTTCC TTCACCCAAA GCAGCAGCAG CTCCAGCACC 
CTTAATAAAT CCAGCTCCTC TCCTGAGCTC CAGACCCTTC CAGAGGCCTT CAGCAAAGCC 
TCGTCCCAGA GCGTGGATCC AGCTCCCACC AGCAAAATCC CTACAGTCCA GGCCTGTGTG 
GAGGCTCCAG GAGAGAGCGC TGGATCCACC GCTGGGACAT CTTGCAGCAG CTCTAGCGTG 
TCCGTGTCCT CCTCCTCCAC CTCCAGAATG AGGCTGGAGT TCCCGCCAGT GCAGACAGGA 
CCCCTGTCTC CTACTGGACA CCGGCCACGG GGACACACCA TATCAGTGTC TGCTCCATCG 
TCCAGGAGAG ACCGCAAAAC ACTGGAGCGG GACGCCAGAG GAGGAGCGAC CAATGTGGAG 
AAGAGCTCCG GACTCAGCCC CAGTTTTGTC TTCCTGCAGC TTTATCACTC GCCGTTCTTC 
GGCAATGAGG CCAACAAACC CCTGCTGCTT CCTAAATCAC AGCTGATTGA CCGTGCAGTG 
AAGGTTTTGG ATCAGATGCC TCCGTACGAC ACTCATAAGA TCGGTGTGGT GTTTGTGGGA 
GCCGGACAGG CCAATAATGA GGTGTCGATC CTGTCTAATG AGTACGGCTC GAAGCGCTAT 
GCTCAGTTCC TGACGGGTCT GGGGAAACTC ATCCACTTGA AAGACTGCGA TCCCGATCAG 
ATCTTCCTTG GTGGACTGGA CCAGTACGGA GACGATGGAG AGTTCACATA CTGCTGGCAT 
GATGACATTA TGCAGGCTAT TTTCCACATC GCCACGCTGA TGCCGAACCG TGAGAGCGAC 
CGCGGCTGCT GCAACAAGAA GCGGCACATC GGCAACGACT TCGTGGTGGT GGTTTATAAC 
GACTCGGGGG AGGACTACAA ACTGGGCACC ATCAAGGGTC AGTTCAACTT TGTGGAAGTG 
CTCATAAAGC CGCTGGACTA CGAGAGTAAC CTGGTGACAC TGCAGTGCCG GAAAGATCTG 
GAGGGTTTGG TGGACACCAG TGTAGCGAAG ATCGTCTCTG ACCGGAACCT TCCCCTGCTA 
GTCAGACAGA TGGCGCTTCA TGCTAACATG GCGTCGCTGG TCCACCAGTG CAGGGCGAAC 
CCATCAGACG CATACGCTTC AAAATGGCTG GCCAGACTGA GACACATCAA GAGGATCAGG 
ACGCGGGCTC AGGAGGAGAT TCAGTCCCGC CCCTCTCACG GCATCTCTCT GACTCAAGGC 
CACGCCTCCA TGCAGAATAA ACCCGCCCAC CAGCCGCCGG GAGCAGCACA GAATCCAGAC 
GCCGGCCAGA GGAAGAGGCT GGTGTCCACC GTCGACGACT TCACTGACTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293248.2
CDS	188..5644
Translation	MNKQPSKESLRDKFKGIFGLGPTRPHHKQADNKPSEYIITLDILMELTSEYSLNHRIRMVNHVCELAKTKRFEEHAVEAIWKAVEDLTQPEQPPEARHAVLVLLRAIIQGQGEWLGPLRAYFFKVILDYQPSNEELPERLEVFKALTENGKDITYLEEEIARFVLLWMENSMSSDFLHVLVNLVKFNSCYLDENVSLMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYSEDAALLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGRELQVVTWDILLSIIDRLLQQIQTMGSPDLKVIVYELLSTIEELYEQNDFHGSSARFFSLVEKCADKRPDASVLTLVSYRAQAIQPAKDGWLQNLLKLMDKFFRNESRTMIRIKVLHILSFVLSTNRQLYEEELIEVVVIPQLGQIAEDRDPSVRKQATQLLVDLAEGCSTHHFSSLLDIIEKVASRPLSCSVEGDRELCVESPLEDVRTAILGLLDILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSAISGAIRLKVFDFLLLMRADSLHRLGVPNKDGALRFSPYCHCDPGESEKRVSEKKPTGSVSPPAGSPTPAAPPSSIRTAYLPYSLAFSVLLQCLKTETDWKVLKLVLDKMSCTIQYKVLILTSPCNIDNLCATLCSMVTDRIILERLKKTPDGFSLTDVQLAVVPVLTAITSYHSYLDQLRQRDLVQCLEKGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDTHEQSSFRARSTSLNERPKSLRTTKVAKQGPSANSPVKDLKDLSAMDAFRSRSISVSEHAVRRMQTSSTTCSLGSADENAVMQADDALKTVHLELTETCLDMMARYVFSNFSALPKRSPIADFLLSGGPSMTWLVGNKLVTITTSGGSRTQALLGLDMTERHGGGEMTRSDPSLHTRQTKEAPAKLESQSSQQINTNTRTRVRSISGGHALRAGPAQSLSPLVASPEGEYSGPLPPPGPPLDVLSSQRGMEEHPPPSACNPSPPLKHKHSLAEFVPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNLPLQELSSVLMAMDGVKEPPAERPTDVSQPQGKPAPIQRSNTDSVVMEESGRSMASVSPAESKEVEEFEAVPSEPIFMNTSTTAPGMMSRSSSTSSQDDDKSTLEEVSEGGIPIDQPPLSLCTPGAQDPELSFTQSSSSSSTLNKSSSSPELQTLPEAFSKASSQSVDPAPTSKIPTVQACVEAPGESAGSTAGTSCSSSSVSVSSSSTSRMRLEFPPVQTGPLSPTGHRPRGHTISVSAPSSRRDRKTLERDARGGATNVEKSSGLSPSFVFLQLYHSPFFGNEANKPLLLPKSQLIDRAVKVLDQMPPYDTHKIGVVFVGAGQANNEVSILSNEYGSKRYAQFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDRGCCNKKRHIGNDFVVVVYNDSGEDYKLGTIKGQFNFVEVLIKPLDYESNLVTLQCRKDLEGLVDTSVAKIVSDRNLPLLVRQMALHANMASLVHQCRANPSDAYASKWLARLRHIKRIRTRAQEEIQSRPSHGISLTQGHASMQNKPAHQPPGAAQNPDAGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_009294973.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294973.2

ORF Insert Sequence:

ATGAATAAAC AGCCCAGTAA AGAGTCGTTG AGGGACAAAT TTAAGGGCAT TTTTGGCCTC 
GGGCCCACTA GGCCGCACCA TAAACAAGCT GACAACAAAC CCTCTGAATA CATCATTACA 
CTGGACATCC TGATGGAGCT CACGTCGGAG TACAGCCTGA ACCATCGCAT CAGGATGGTT 
AATCATGTTT GTGAATTGGC GAAAACGAAG AGGTTTGAGG AGCATGCAGT GGAGGCCATC 
TGGAAGGCGG TGGAGGACCT GACGCAGCCG GAGCAGCCAC CTGAAGCCCG TCATGCGGTG 
CTGGTGCTCT TAAGGGCGAT CATACAGGGA CAGGGCGAGT GGTTGGGTCC GCTGAGAGCT 
TACTTCTTTA AGGTCATTCT GGACTATCAG CCTTCTAATG AAGAGCTGCC GGAGAGACTG 
GAGGTGTTCA AGGCCCTCAC AGAGAACGGA AAGGACATCA CATATCTAGA GGAGGAGATT 
GCTCGGTTTG TCCTGTTGTG GATGGAGAAC AGCATGTCCT CAGATTTCCT GCACGTCCTC 
GTCAACCTTG TCAAATTCAA CTCCTGCTAC CTGGATGAGA ACGTTTCGCT TATGGTCCAG 
AAAATCTGCC TTCTGTGCAA CCGGACGACA TCCTCCACAG ACATTGAGGT TGCGCTGCAG 
GTGCTGGACG CGGTGGTGTG TTATAACTGT CTGCCATCAG ACTCTCTAAC AGTCTTCATC 
ATCACTCTGT GCCGAACTGT AAATGTAAAG GAGTTCTGCG AGTCCTGCTG GAAGTTGATG 
CGCAAAGTGT TGGGAACTCA TCTGGGTCAC AGTGCCATAT ACACCATGTG CCGAATCATG 
GAGGAGAGGG TGTACTCCGA GGACGCCGCT CTCCTCCGCG GTGCTGTGTT TTTTGTTGGG 
ATGGCGCTGT GGGGCGCTCA CAGACTCCCC GCCCTCAAAA ACACACCTAC GCTGGTCCTG 
CCTTCCTTCT ACAAGGCCAT GAGCTGTGCT AATGAGGTGG TGTCCTATGA GATCGTGCTC 
TCCATCACGC GCCTCATTAA AAAATACGGC CGCGAGCTGC AGGTGGTGAC CTGGGACATC 
CTGCTGTCCA TCATAGACAG ACTCCTGCAG CAGATACAGA CGATGGGCAG TCCTGACCTG 
AAGGTGATCG TCTATGAGCT GCTGAGCACC ATCGAGGAGC TTTACGAGCA GAACGACTTC 
CACGGCTCCT CGGCCCGCTT CTTCAGCCTG GTGGAGAAGT GTGCGGATAA ACGTCCTGAT 
GCATCTGTGC TCACGCTGGT CTCCTACAGA GCTCAGGCCA TCCAGCCGGC CAAGGACGGA 
TGGCTGCAGA ACCTGCTCAA ACTCATGGAC AAGTTCTTCA GGAATGAGAG CCGCACTATG 
ATCAGGATCA AGGTTCTGCA CATCTTGTCG TTCGTCCTCA GCACAAACCG TCAGCTGTAT 
GAGGAGGAGC TGATTGAGGT GGTGGTCATC CCTCAGCTGG GTCAGATAGC TGAGGATCGT 
GACCCGTCAG TCAGGAAGCA GGCCACACAG CTGCTGGTGG ATCTGGCCGA GGGCTGCAGC 
ACACACCACT TCAGCAGTCT GCTGGACATC ATCGAGAAGG TGGCCAGTCG TCCTCTGAGC 
TGCTCTGTAG AGGGCGACAG AGAACTGTGT GTGGAGTCGC CGCTGGAGGA CGTGCGCACC 
GCTATACTGG GCCTGCTGGA CATCCTGCAG AGCAAACTCT ATAGTCTGCC TGCCAGTCAT 
GCCAGTCGAG TGTATGAGCT GCTCATCTCA CACCTGCAGC TGCACTACAA GAACAAATAC 
TGCAGCGCCA TCAGCGGAGC CATACGCCTC AAGGTGTTTG ATTTCCTGCT GCTGATGAGG 
GCTGATTCTC TACATCGTCT CGGGGTCCCC AATAAAGACG GAGCTCTGAG ATTCAGCCCG 
TACTGCCACT GTGACCCAGG GGAGTCCGAG AAGAGAGTGT CAGAGAAGAA GCCGACAGGA 
AGTGTGTCTC CACCGGCAGG AAGTCCCACA CCGGCAGCTC CGCCCTCCTC CATCCGCACC 
GCTTACCTGC CCTACAGTCT GGCTTTCAGT GTGCTGCTGC AGTGCCTCAA GACGGAGACC 
GACTGGAAGG TCCTGAAGCT GGTTCTGGAT AAGATGTCCT GCACCATCCA GTATAAAGTG 
CTGATCCTGA CGTCTCCATG TAACATTGAT AATCTGTGCG CAACGCTCTG CTCGATGGTG 
ACGGACCGCA TTATTTTGGA GCGTCTGAAG AAAACCCCTG ATGGATTTTC CCTCACTGAC 
GTTCAGCTCG CTGTGGTTCC TGTTCTCACG GCCATCACGT CCTACCACAG CTATCTGGAT 
CAGCTCCGAC AGAGGGACCT GGTGCAGTGT CTGGAGAAGG GTCTGATCTA CCGCTGTGCC 
AAGCAGTGTG TGGTGGCCCT GACAATGTGC ACAGTGGAGA TGCCCGACAT CATGATCAAA 
CTACTGCCCG CCCTTATCGT CAAGCTCACG CACATCTCGG CAACCGTTGC CATGGCCTCA 
CCCATGCTGG AGTTCCTGTC CACATTAGTG CGGCTCCCAC ATCTGTATGC TAACTTCGTA 
GCTGAGCAGT ATGTTAGCGT GTTTGCCATA TCGCTGCCGT ACACCAATCC TTCAAAGTTT 
AATCAGTACA TAGTGTCTCT GGCTCATCAT GTGATCGCCA TGTGGTTCAT CCGCTGCCGA 
CTGCCTTTCA GGAAAGATTT TGTTCAGTAC ATCACCAAGG GTCTTCGTTC TAACGCTCTC 
CTGCCGTTTG ACGACACACA CGAGCAGAGC AGTTTCAGAG CACGCAGCAC CAGCCTGAAC 
GAGAGGCCCA AGAGTTTGCG GACGACCAAA GTGGCGAAGC AAGGCCCCAG CGCAAACTCT 
CCCGTTAAAG ACCTGAAAGA CCTTTCGGCC ATGGACGCCT TCCGCTCCCG CAGCATCAGT 
GTGTCCGAGC ATGCTGTGCG CAGGATGCAG ACGTCCAGCA CCACCTGCAG TCTGGGCTCA 
GCTGATGAGA ACGCTGTGAT GCAGGCGGAC GACGCTCTGA AGACAGTCCA TCTGGAGCTC 
ACAGAGACCT GCCTGGACAT GATGGCCAGA TACGTCTTCT CCAACTTCTC TGCTCTGCCC 
AAGAGGTCTC CCATCGCTGA TTTCCTGTTA TCTGGTGGTC CTAGTATGAC GTGGCTGGTT 
GGGAATAAGT TAGTGACCAT AACCACCAGC GGCGGCTCGC GAACACAAGC TCTTCTGGGT 
CTGGACATGA CAGAGAGACA TGGAGGAGGA GAAATGACCA GGTCTGACCC GTCTCTGCAC 
ACCCGACAGA CCAAAGAAGC TCCCGCTAAA CTAGAGTCTC AGTCCAGCCA ACAGATCAAC 
ACCAACACAC GCACACGAGT CCGCTCTATA TCAGGTGGTC ATGCTCTGCG GGCCGGGCCA 
GCTCAGAGTC TTAGTCCTCT GGTGGCGTCT CCTGAAGGCG AGTACAGCGG CCCGCTGCCT 
CCTCCCGGAC CCCCGCTGGA CGTCCTGAGC TCTCAGAGGG GGATGGAGGA GCACCCGCCA 
CCCTCCGCCT GCAACCCATC ACCACCGCTC AAGCACAAGC ACAGCCTGGC AGAGTTTGTG 
CCCATGCTGA CGCAGGGCTG GGCCGAGATC TTCATCCGCA GGCCCTCAGG GAACACGAGC 
TGGCTGATGT GTCTGGAGAA CCCGCCAAGC CCGTTCTCCT CTGAGCTGGG TAATCTGCCT 
CTGCAGGAGC TCTCCAGCGT CCTCATGGCC ATGGACGGAG TGAAGGAGCC ACCGGCCGAG 
CGTCCCACAG ACGTGTCCCA GCCACAGGGC AAACCGGCTC CCATCCAGCG ATCCAACACT 
GATTCAGTAG TGATGGAGGA AAGTGGGCGG AGTATGGCGT CTGTTTCACC TGCTGAGTCT 
AAAGAGGTGG AGGAGTTTGA GGCCGTTCCT TCTGAACCCA TCTTCATGAA CACCAGCACG 
ACTGCACCCG GCATGATGAG CAGATCCTCC TCCACATCCA GTCAGGATGA TGATAAGTCC 
ACCCTGGAGG AGGTCAGTGA AGGAGGAATC CCCATCGACC AGCCTCCGCT CAGCCTCTGC 
ACCCCTGGAG CCCAGGACCC CGAGCTTTCC TTCACCCAAA GCAGCAGCAG CTCCAGCACC 
CTTAATAAAT CCAGCTCCTC TCCTGAGCTC CAGACCCTTC CAGAGGCCTT CAGCAAAGCC 
TCGTCCCAGA GCGTGGATCC AGCTCCCACC AGCAAAATCC CTACAGTCCA GGCCTGTGTG 
GAGGCTCCAG GAGAGAGCGC TGGATCCACC GCTGGGACAT CTTGCAGCAG CTCTAGCGTG 
TCCGTGTCCT CCTCCTCCAC CTCCAGAATG AGGCTGGAGT TCCCGCCAGT GCAGACAGGA 
CCCCTGTCTC CTACTGGACA CCGGCCACGG GGACACACCA TATCAGTGTC TGCTCCATCG 
TCCAGGAGAG ACCGCAAAAC ACTGGAGCGG GACGCCAGAG GAGGAGCGAC CAATGTGGAG 
AAGAGCTCCG GACTCAGCCC CAGTTTTGTC TTCCTGCAGC TTTATCACTC GCCGTTCTTC 
GGCAATGAGG CCAACAAACC CCTGCTGCTT CCTAAATCAC AGCTGATTGA CCGTGCAGTG 
AAGGTTTTGG ATCAGATGCC TCCGTACGAC ACTCATAAGA TCGGTGTGGT GTTTGTGGGA 
GCCGGACAGG CCAATAATGA GGTGTCGATC CTGTCTAATG AGTACGGCTC GAAGCGCTAT 
GCTCAGTTCC TGACGGGTCT GGGGAAACTC ATCCACTTGA AAGACTGCGA TCCCGATCAG 
ATCTTCCTTG GTGGACTGGA CCAGTACGGA GACGATGGAG AGTTCACATA CTGCTGGCAT 
GATGACATTA TGCAGGCTAT TTTCCACATC GCCACGCTGA TGCCGAACCG TGAGAGCGAC 
CGCGGCTGCT GCAACAAGAA GCGGCACATC GGCAACGACT TCGTGGTGGT GGTTTATAAC 
GACTCGGGGG AGGACTACAA ACTGGGCACC ATCAAGGGTC AGTTCAACTT TGTGGAAGTG 
CTCATAAAGC CGCTGGACTA CGAGAGTAAC CTGGTGACAC TGCAGTGCCG GAAAGATCTG 
GAGGGTTTGG TGGACACCAG TGTAGCGAAG ATCGTCTCTG ACCGGAACCT TCCCCTGCTA 
GTCAGACAGA TGGCGCTTCA TGCTAACATG GCGTCGCTGG TCCACCAGTG CAGGGCGAAC 
CCATCAGACG CATACGCTTC AAAATGGCTG GCCAGACTGA GACACATCAA GAGGATCAGG 
ACGCGGGCTC AGGAGGAGAT TCAGTCCCGC CCCTCTCACG GCATCTCTCT GACTCAAGGC 
CACGCCTCCA TGCAGAATAA ACCCGCCCAC CAGCCGCCGG GAGCAGCACA GAATCCAGAC 
GCCGGCCAGA GGAAGAGGCT GGTGTCCACC GTCGACGACT TCACTGACTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_017355410.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210899.1 Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.

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CloneID	ODa53726
Clone ID Related Accession (Same CDS sequence)	XM_017355410.1
Accession Version	XM_017355410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tuberin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) and transcript sequence (GDQH01012854.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 520 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAATAAAC AGCCCAGTAA AGAGTCGTTG AGGGACAAAT TTAAGGGCAT TTTTGGCCTC 
GGGCCCACTA GGCCGCACCA TAAACAAGCT GACAACAAAC CCTCTGAATA CATCATTACA 
CTGGACATCC TGATGGAGCT CACGTCGGAG TACAGCCTGA ACCATCGCAT CAGGATGGTT 
AATCATGTTT GTGAATTGGC GAAAACGAAG AGGTTTGAGG AGCATGCAGT GGAGGCCATC 
TGGAAGGCGG TGGAGGACCT GACGCAGCCG GAGCAGCCAC CTGAAGCCCG TCATGCGGTG 
CTGGTGCTCT TAAGGGCGAT CATACAGGGA CAGGGCGAGT GGTTGGGTCC GCTGAGAGCT 
TACTTCTTTA AGGTCATTCT GGACTATCAG CCTTCTAATG AAGAGCTGCC GGAGAGACTG 
GAGGTGTTCA AGGCCCTCAC AGAGAACGGA AAGGACATCA CATATCTAGA GGAGGAGATT 
GCTCGGTTTG TCCTGTTGTG GATGGAGAAC AGCATGTCCT CAGATTTCCT GCACGTCCTC 
GTCAACCTTG TCAAATTCAA CTCCTGCTAC CTGGATGAGA ACGTTTCGCT TATGGTCCAG 
AAAATCTGCC TTCTGTGCAA CCGGACGACA TCCTCCACAG ACATTGAGGT TGCGCTGCAG 
GTGCTGGACG CGGTGGTGTG TTATAACTGT CTGCCATCAG ACTCTCTAAC AGTCTTCATC 
ATCACTCTGT GCCGAACTGT AAATGTAAAG GAGTTCTGCG AGTCCTGCTG GAAGTTGATG 
CGCAAAGTGT TGGGAACTCA TCTGGGTCAC AGTGCCATAT ACACCATGTG CCGAATCATG 
GAGGAGAGGG TGTACTCCGA GGACGCCGCT CTCCTCCGCG GTGCTGTGTT TTTTGTTGGG 
ATGGCGCTGT GGGGCGCTCA CAGACTCCCC GCCCTCAAAA ACACACCTAC GCTGGTCCTG 
CCTTCCTTCT ACAAGGCCAT GAGCTGTGCT AATGAGGTGG TGTCCTATGA GATCGTGCTC 
TCCATCACGC GCCTCATTAA AAAATACGGC CGCGAGCTGC AGGTGGTGAC CTGGGACATC 
CTGCTGTCCA TCATAGACAG ACTCCTGCAG CAGATACAGA CGATGGGCAG TCCTGACCTG 
AAGGTGATCG TCTATGAGCT GCTGAGCACC ATCGAGGAGC TTTACGAGCA GAACGACTTC 
CACGGCTCCT CGGCCCGCTT CTTCAGCCTG GTGGAGAAGT GTGCGGATAA ACGTCCTGAT 
GCATCTGTGC TCACGCTGGT CTCCTACAGA GCTCAGGCCA TCCAGCCGGC CAAGGACGGA 
TGGCTGCAGA ACCTGCTCAA ACTCATGGAC AAGTTCTTCA GGAATGAGAG CCGCACTATG 
ATCAGGATCA AGGTTCTGCA CATCTTGTCG TTCGTCCTCA GCACAAACCG TCAGCTGTAT 
GAGGAGGAGC TGATTGAGGT GGTGGTCATC CCTCAGCTGG GTCAGATAGC TGAGGATCGT 
GACCCGTCAG TCAGGAAGCA GGCCACACAG CTGCTGGTGG ATCTGGCCGA GGGCTGCAGC 
ACACACCACT TCAGCAGTCT GCTGGACATC ATCGAGAAGG TGGCCAGTCG TCCTCTGAGC 
TGCTCTGTAG AGGGCGACAG AGAACTGTGT GTGGAGTCGC CGCTGGAGGA CGTGCGCACC 
GCTATACTGG GCCTGCTGGA CATCCTGCAG AGCAAACTCT ATAGTCTGCC TGCCAGTCAT 
GCCAGTCGAG TGTATGAGCT GCTCATCTCA CACCTGCAGC TGCACTACAA GAACAAATAC 
TGCAGCGCCA TCAGCGGAGC CATACGCCTC AAGGTGTTTG ATTTCCTGCT GCTGATGAGG 
GCTGATTCTC TACATCGTCT CGGGGTCCCC AATAAAGACG GAGCTCTGAG ATTCAGCCCG 
TACTGCCACT GTGACCCAGG GGAGTCCGAG AAGAGAGTGT CAGAGAAGAA GCCGACAGGA 
AGTGTGTCTC CACCGGCAGG AAGTCCCACA CCGGCAGCTC CGCCCTCCTC CATCCGCACC 
GCTTACCTGC CCTACAGTCT GGCTTTCAGT GTGCTGCTGC AGTGCCTCAA GACGGAGACC 
GACTGGAAGG TCCTGAAGCT GGTTCTGGAT AAGATGTCCT GCACCATCCA GTATAAAGTG 
CTGATCCTGA CGTCTCCATG TAACATTGAT AATCTGTGCG CAACGCTCTG CTCGATGGTG 
ACGGACCGCA TTATTTTGGA GCGTCTGAAG AAAACCCCTG ATGGATTTTC CCTCACTGAC 
GTTCAGCTCG CTGTGGTTCC TGTTCTCACG GCCATCACGT CCTACCACAG CTATCTGGAT 
CAGCTCCGAC AGAGGGACCT GGTGCAGTGT CTGGAGAAGG GTCTGATCTA CCGCTGTGCC 
AAGCAGTGTG TGGTGGCCCT GACAATGTGC ACAGTGGAGA TGCCCGACAT CATGATCAAA 
CTACTGCCCG CCCTTATCGT CAAGCTCACG CACATCTCGG CAACCGTTGC CATGGCCTCA 
CCCATGCTGG AGTTCCTGTC CACATTAGTG CGGCTCCCAC ATCTGTATGC TAACTTCGTA 
GCTGAGCAGT ATGTTAGCGT GTTTGCCATA TCGCTGCCGT ACACCAATCC TTCAAAGTTT 
AATCAGTACA TAGTGTCTCT GGCTCATCAT GTGATCGCCA TGTGGTTCAT CCGCTGCCGA 
CTGCCTTTCA GGAAAGATTT TGTTCAGTAC ATCACCAAGG GTCTTCGTTC TAACGCTCTC 
CTGCCGTTTG ACGACACACA CGAGCAGAGC AGTTTCAGAG CACGCAGCAC CAGCCTGAAC 
GAGAGGCCCA AGAGGATGCA GACGTCCAGC ACCACCTGCA GTCTGGGCTC AGCTGATGAG 
AACGCTGTGA TGCAGGCGGA CGACGCTCTG AAGACAGTCC ATCTGGAGCT CACAGAGACC 
TGCCTGGACA TGATGGCCAG ATACGTCTTC TCCAACTTCT CTGCTCTGCC CAAGAGGTCT 
CCCATCGCTG ATTTCCTGTT ATCTGGTGGT CCTAGTATGA CGTGGCTGGT TGGGAATAAG 
TTAGTGACCA TAACCACCAG CGGCGGCTCG CGAACACAAG CTCTTCTGGG TCTGGACATG 
ACAGAGAGAC ATGGAGGAGG AGAAATGACC AGGTCTGACC CGTCTCTGCA CACCCGACAG 
ACCAAAGAAG CTCCCGCTAA ACTAGAGTCT CAGTCCAGCC AACAGATCAA CACCAACACA 
CGCACACGAG TCCGCTCTAT ATCAGGTGGT CATGCTCTGC GGGCCGGGCC AGCTCAGAGT 
CTTAGTCCTC TGGTGGCGTC TCCTGAAGGC GAGTACAGCG GCCCGCTGCC TCCTCCCGGA 
CCCCCGCTGG ACGTCCTGAG CTCTCAGAGG GGGATGGAGG AGCACCCGCC ACCCTCCGCC 
TGCAACCCAT CACCACCGCT CAAGCACAAG CACAGCCTGG CAGAGTTTGT GCCCATGCTG 
ACGCAGGGCT GGGCCGAGAT CTTCATCCGC AGGCCCTCAG GGAACACGAG CTGGCTGATG 
TGTCTGGAGA ACCCGCCAAG CCCGTTCTCC TCTGAGCTGG GTAATCTGCC TCTGCAGGAG 
CTCTCCAGCG TCCTCATGGC CATGGACGGA GTGAAGGAGC CACCGGCCGA GCGTCCCACA 
GACGTGTCCC AGCCACAGGG CAAACCGGCT CCCATCCAGC GATCCAACAC TGTGGCCTCT 
CTCAGCTCTC TCTGCTCCTC TGAATCATCC GGCAGGTTTC ACAGGAGTAT ATCCTGGGCA 
GATTCAGTAG TGATGGAGGA AAGTGGGCGG AGTATGGCGT CTGTTTCACC TGCTGAGTCT 
AAAGAGGTGG AGGAGTTTGA GGCCGTTCCT TCTGAACCCA TCTTCATGAA CACCAGCACG 
ACTGCACCCG GCATGATGAG CAGATCCTCC TCCACATCCA GTCAGGATGA TGATAAGTCC 
ACCCTGGAGG AGGTCAGTGA AGGAGGAATC CCCATCGACC AGCCTCCGCT CAGCCTCTGC 
ACCCCTGGAG CCCAGGACCC CGAGCTTTCC TTCACCCAAA GCAGCAGCAG CTCCAGCACC 
CTTAATAAAT CCAGCTCCTC TCCTGAGCTC CAGACCCTTC CAGAGGCCTT CAGCAAAGCC 
TCGTCCCAGA GCGTGGATCC AGCTCCCACC AGCAAAATCC CTACAGTCCA GGCCTGTGTG 
GAGGCTCCAG GAGAGAGCGC TGGATCCACC GCTGGGACAT CTTGCAGCAG CTCTAGCGTG 
TCCGTGTCCT CCTCCTCCAC CTCCAGAATG AGGCTGGAGT TCCCGCCAGT GCAGACAGGA 
CCCCTGTCTC CTACTGGACA CCGGCCACGG GGACACACCA TATCAGTGTC TGCTCCATCG 
TCCAGGAGAG ACCGCAAAAC ACTGGAGCGG GACGCCAGAG GAGGAGCGAC CAATGTGGAG 
AAGAGCTCCG GACTCAGCCC CAGTTTTGTC TTCCTGCAGC TTTATCACTC GCCGTTCTTC 
GGCAATGAGG CCAACAAACC CCTGCTGCTT CCTAAATCAC AGCTGATTGA CCGTGCAGTG 
AAGGTTTTGG ATCAGATGCC TCCGTACGAC ACTCATAAGA TCGGTGTGGT GTTTGTGGGA 
GCCGGACAGG CCAATAATGA GGTGTCGATC CTGTCTAATG AGTACGGCTC GAAGCGCTAT 
GCTCAGTTCC TGACGGGTCT GGGGAAACTC ATCCACTTGA AAGACTGCGA TCCCGATCAG 
ATCTTCCTTG GTGGACTGGA CCAGTACGGA GACGATGGAG AGTTCACATA CTGCTGGCAT 
GATGACATTA TGCAGGCTAT TTTCCACATC GCCACGCTGA TGCCGAACCG TGAGAGCGAC 
CGCGGCTGCT GCAACAAGAA GCGGCACATC GGCAACGACT TCGTGGTGGT GGTTTATAAC 
GACTCGGGGG AGGACTACAA ACTGGGCACC ATCAAGGGTC AGTTCAACTT TGTGGAAGTG 
CTCATAAAGC CGCTGGACTA CGAGAGTAAC CTGGTGACAC TGCAGTGCCG GAAAGATCTG 
GAGGGTTTGG TGGACACCAG TGTAGCGAAG ATCGTCTCTG ACCGGAACCT TCCCCTGCTA 
GTCAGACAGA TGGCGCTTCA TGCTAACATG GCGTCGCTGG TCCACCAGTG CAGGGCGAAC 
CCATCAGACG CATACGCTTC AAAATGGCTG GCCAGACTGA GACACATCAA GAGGATCAGG 
ACGCGGGCTC AGGAGGAGAT TCAGTCCCGC CCCTCTCACG GCATCTCTCT GACTCAAGGC 
CACGCCTCCA TGCAGAATAA ACCCGCCCAC CAGCCGCCGG GAGCAGCACA GAATCCAGAC 
GCCGGCCAGA GGAAGAGGCT GGTGTCCACC GTCGACGACT TCACTGACTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210899.1
CDS	188..5584
Translation	MNKQPSKESLRDKFKGIFGLGPTRPHHKQADNKPSEYIITLDILMELTSEYSLNHRIRMVNHVCELAKTKRFEEHAVEAIWKAVEDLTQPEQPPEARHAVLVLLRAIIQGQGEWLGPLRAYFFKVILDYQPSNEELPERLEVFKALTENGKDITYLEEEIARFVLLWMENSMSSDFLHVLVNLVKFNSCYLDENVSLMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYSEDAALLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGRELQVVTWDILLSIIDRLLQQIQTMGSPDLKVIVYELLSTIEELYEQNDFHGSSARFFSLVEKCADKRPDASVLTLVSYRAQAIQPAKDGWLQNLLKLMDKFFRNESRTMIRIKVLHILSFVLSTNRQLYEEELIEVVVIPQLGQIAEDRDPSVRKQATQLLVDLAEGCSTHHFSSLLDIIEKVASRPLSCSVEGDRELCVESPLEDVRTAILGLLDILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSAISGAIRLKVFDFLLLMRADSLHRLGVPNKDGALRFSPYCHCDPGESEKRVSEKKPTGSVSPPAGSPTPAAPPSSIRTAYLPYSLAFSVLLQCLKTETDWKVLKLVLDKMSCTIQYKVLILTSPCNIDNLCATLCSMVTDRIILERLKKTPDGFSLTDVQLAVVPVLTAITSYHSYLDQLRQRDLVQCLEKGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDTHEQSSFRARSTSLNERPKRMQTSSTTCSLGSADENAVMQADDALKTVHLELTETCLDMMARYVFSNFSALPKRSPIADFLLSGGPSMTWLVGNKLVTITTSGGSRTQALLGLDMTERHGGGEMTRSDPSLHTRQTKEAPAKLESQSSQQINTNTRTRVRSISGGHALRAGPAQSLSPLVASPEGEYSGPLPPPGPPLDVLSSQRGMEEHPPPSACNPSPPLKHKHSLAEFVPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNLPLQELSSVLMAMDGVKEPPAERPTDVSQPQGKPAPIQRSNTVASLSSLCSSESSGRFHRSISWADSVVMEESGRSMASVSPAESKEVEEFEAVPSEPIFMNTSTTAPGMMSRSSSTSSQDDDKSTLEEVSEGGIPIDQPPLSLCTPGAQDPELSFTQSSSSSSTLNKSSSSPELQTLPEAFSKASSQSVDPAPTSKIPTVQACVEAPGESAGSTAGTSCSSSSVSVSSSSTSRMRLEFPPVQTGPLSPTGHRPRGHTISVSAPSSRRDRKTLERDARGGATNVEKSSGLSPSFVFLQLYHSPFFGNEANKPLLLPKSQLIDRAVKVLDQMPPYDTHKIGVVFVGAGQANNEVSILSNEYGSKRYAQFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDRGCCNKKRHIGNDFVVVVYNDSGEDYKLGTIKGQFNFVEVLIKPLDYESNLVTLQCRKDLEGLVDTSVAKIVSDRNLPLLVRQMALHANMASLVHQCRANPSDAYASKWLARLRHIKRIRTRAQEEIQSRPSHGISLTQGHASMQNKPAHQPPGAAQNPDAGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_017355410.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355410.1

ORF Insert Sequence:

ATGAATAAAC AGCCCAGTAA AGAGTCGTTG AGGGACAAAT TTAAGGGCAT TTTTGGCCTC 
GGGCCCACTA GGCCGCACCA TAAACAAGCT GACAACAAAC CCTCTGAATA CATCATTACA 
CTGGACATCC TGATGGAGCT CACGTCGGAG TACAGCCTGA ACCATCGCAT CAGGATGGTT 
AATCATGTTT GTGAATTGGC GAAAACGAAG AGGTTTGAGG AGCATGCAGT GGAGGCCATC 
TGGAAGGCGG TGGAGGACCT GACGCAGCCG GAGCAGCCAC CTGAAGCCCG TCATGCGGTG 
CTGGTGCTCT TAAGGGCGAT CATACAGGGA CAGGGCGAGT GGTTGGGTCC GCTGAGAGCT 
TACTTCTTTA AGGTCATTCT GGACTATCAG CCTTCTAATG AAGAGCTGCC GGAGAGACTG 
GAGGTGTTCA AGGCCCTCAC AGAGAACGGA AAGGACATCA CATATCTAGA GGAGGAGATT 
GCTCGGTTTG TCCTGTTGTG GATGGAGAAC AGCATGTCCT CAGATTTCCT GCACGTCCTC 
GTCAACCTTG TCAAATTCAA CTCCTGCTAC CTGGATGAGA ACGTTTCGCT TATGGTCCAG 
AAAATCTGCC TTCTGTGCAA CCGGACGACA TCCTCCACAG ACATTGAGGT TGCGCTGCAG 
GTGCTGGACG CGGTGGTGTG TTATAACTGT CTGCCATCAG ACTCTCTAAC AGTCTTCATC 
ATCACTCTGT GCCGAACTGT AAATGTAAAG GAGTTCTGCG AGTCCTGCTG GAAGTTGATG 
CGCAAAGTGT TGGGAACTCA TCTGGGTCAC AGTGCCATAT ACACCATGTG CCGAATCATG 
GAGGAGAGGG TGTACTCCGA GGACGCCGCT CTCCTCCGCG GTGCTGTGTT TTTTGTTGGG 
ATGGCGCTGT GGGGCGCTCA CAGACTCCCC GCCCTCAAAA ACACACCTAC GCTGGTCCTG 
CCTTCCTTCT ACAAGGCCAT GAGCTGTGCT AATGAGGTGG TGTCCTATGA GATCGTGCTC 
TCCATCACGC GCCTCATTAA AAAATACGGC CGCGAGCTGC AGGTGGTGAC CTGGGACATC 
CTGCTGTCCA TCATAGACAG ACTCCTGCAG CAGATACAGA CGATGGGCAG TCCTGACCTG 
AAGGTGATCG TCTATGAGCT GCTGAGCACC ATCGAGGAGC TTTACGAGCA GAACGACTTC 
CACGGCTCCT CGGCCCGCTT CTTCAGCCTG GTGGAGAAGT GTGCGGATAA ACGTCCTGAT 
GCATCTGTGC TCACGCTGGT CTCCTACAGA GCTCAGGCCA TCCAGCCGGC CAAGGACGGA 
TGGCTGCAGA ACCTGCTCAA ACTCATGGAC AAGTTCTTCA GGAATGAGAG CCGCACTATG 
ATCAGGATCA AGGTTCTGCA CATCTTGTCG TTCGTCCTCA GCACAAACCG TCAGCTGTAT 
GAGGAGGAGC TGATTGAGGT GGTGGTCATC CCTCAGCTGG GTCAGATAGC TGAGGATCGT 
GACCCGTCAG TCAGGAAGCA GGCCACACAG CTGCTGGTGG ATCTGGCCGA GGGCTGCAGC 
ACACACCACT TCAGCAGTCT GCTGGACATC ATCGAGAAGG TGGCCAGTCG TCCTCTGAGC 
TGCTCTGTAG AGGGCGACAG AGAACTGTGT GTGGAGTCGC CGCTGGAGGA CGTGCGCACC 
GCTATACTGG GCCTGCTGGA CATCCTGCAG AGCAAACTCT ATAGTCTGCC TGCCAGTCAT 
GCCAGTCGAG TGTATGAGCT GCTCATCTCA CACCTGCAGC TGCACTACAA GAACAAATAC 
TGCAGCGCCA TCAGCGGAGC CATACGCCTC AAGGTGTTTG ATTTCCTGCT GCTGATGAGG 
GCTGATTCTC TACATCGTCT CGGGGTCCCC AATAAAGACG GAGCTCTGAG ATTCAGCCCG 
TACTGCCACT GTGACCCAGG GGAGTCCGAG AAGAGAGTGT CAGAGAAGAA GCCGACAGGA 
AGTGTGTCTC CACCGGCAGG AAGTCCCACA CCGGCAGCTC CGCCCTCCTC CATCCGCACC 
GCTTACCTGC CCTACAGTCT GGCTTTCAGT GTGCTGCTGC AGTGCCTCAA GACGGAGACC 
GACTGGAAGG TCCTGAAGCT GGTTCTGGAT AAGATGTCCT GCACCATCCA GTATAAAGTG 
CTGATCCTGA CGTCTCCATG TAACATTGAT AATCTGTGCG CAACGCTCTG CTCGATGGTG 
ACGGACCGCA TTATTTTGGA GCGTCTGAAG AAAACCCCTG ATGGATTTTC CCTCACTGAC 
GTTCAGCTCG CTGTGGTTCC TGTTCTCACG GCCATCACGT CCTACCACAG CTATCTGGAT 
CAGCTCCGAC AGAGGGACCT GGTGCAGTGT CTGGAGAAGG GTCTGATCTA CCGCTGTGCC 
AAGCAGTGTG TGGTGGCCCT GACAATGTGC ACAGTGGAGA TGCCCGACAT CATGATCAAA 
CTACTGCCCG CCCTTATCGT CAAGCTCACG CACATCTCGG CAACCGTTGC CATGGCCTCA 
CCCATGCTGG AGTTCCTGTC CACATTAGTG CGGCTCCCAC ATCTGTATGC TAACTTCGTA 
GCTGAGCAGT ATGTTAGCGT GTTTGCCATA TCGCTGCCGT ACACCAATCC TTCAAAGTTT 
AATCAGTACA TAGTGTCTCT GGCTCATCAT GTGATCGCCA TGTGGTTCAT CCGCTGCCGA 
CTGCCTTTCA GGAAAGATTT TGTTCAGTAC ATCACCAAGG GTCTTCGTTC TAACGCTCTC 
CTGCCGTTTG ACGACACACA CGAGCAGAGC AGTTTCAGAG CACGCAGCAC CAGCCTGAAC 
GAGAGGCCCA AGAGGATGCA GACGTCCAGC ACCACCTGCA GTCTGGGCTC AGCTGATGAG 
AACGCTGTGA TGCAGGCGGA CGACGCTCTG AAGACAGTCC ATCTGGAGCT CACAGAGACC 
TGCCTGGACA TGATGGCCAG ATACGTCTTC TCCAACTTCT CTGCTCTGCC CAAGAGGTCT 
CCCATCGCTG ATTTCCTGTT ATCTGGTGGT CCTAGTATGA CGTGGCTGGT TGGGAATAAG 
TTAGTGACCA TAACCACCAG CGGCGGCTCG CGAACACAAG CTCTTCTGGG TCTGGACATG 
ACAGAGAGAC ATGGAGGAGG AGAAATGACC AGGTCTGACC CGTCTCTGCA CACCCGACAG 
ACCAAAGAAG CTCCCGCTAA ACTAGAGTCT CAGTCCAGCC AACAGATCAA CACCAACACA 
CGCACACGAG TCCGCTCTAT ATCAGGTGGT CATGCTCTGC GGGCCGGGCC AGCTCAGAGT 
CTTAGTCCTC TGGTGGCGTC TCCTGAAGGC GAGTACAGCG GCCCGCTGCC TCCTCCCGGA 
CCCCCGCTGG ACGTCCTGAG CTCTCAGAGG GGGATGGAGG AGCACCCGCC ACCCTCCGCC 
TGCAACCCAT CACCACCGCT CAAGCACAAG CACAGCCTGG CAGAGTTTGT GCCCATGCTG 
ACGCAGGGCT GGGCCGAGAT CTTCATCCGC AGGCCCTCAG GGAACACGAG CTGGCTGATG 
TGTCTGGAGA ACCCGCCAAG CCCGTTCTCC TCTGAGCTGG GTAATCTGCC TCTGCAGGAG 
CTCTCCAGCG TCCTCATGGC CATGGACGGA GTGAAGGAGC CACCGGCCGA GCGTCCCACA 
GACGTGTCCC AGCCACAGGG CAAACCGGCT CCCATCCAGC GATCCAACAC TGTGGCCTCT 
CTCAGCTCTC TCTGCTCCTC TGAATCATCC GGCAGGTTTC ACAGGAGTAT ATCCTGGGCA 
GATTCAGTAG TGATGGAGGA AAGTGGGCGG AGTATGGCGT CTGTTTCACC TGCTGAGTCT 
AAAGAGGTGG AGGAGTTTGA GGCCGTTCCT TCTGAACCCA TCTTCATGAA CACCAGCACG 
ACTGCACCCG GCATGATGAG CAGATCCTCC TCCACATCCA GTCAGGATGA TGATAAGTCC 
ACCCTGGAGG AGGTCAGTGA AGGAGGAATC CCCATCGACC AGCCTCCGCT CAGCCTCTGC 
ACCCCTGGAG CCCAGGACCC CGAGCTTTCC TTCACCCAAA GCAGCAGCAG CTCCAGCACC 
CTTAATAAAT CCAGCTCCTC TCCTGAGCTC CAGACCCTTC CAGAGGCCTT CAGCAAAGCC 
TCGTCCCAGA GCGTGGATCC AGCTCCCACC AGCAAAATCC CTACAGTCCA GGCCTGTGTG 
GAGGCTCCAG GAGAGAGCGC TGGATCCACC GCTGGGACAT CTTGCAGCAG CTCTAGCGTG 
TCCGTGTCCT CCTCCTCCAC CTCCAGAATG AGGCTGGAGT TCCCGCCAGT GCAGACAGGA 
CCCCTGTCTC CTACTGGACA CCGGCCACGG GGACACACCA TATCAGTGTC TGCTCCATCG 
TCCAGGAGAG ACCGCAAAAC ACTGGAGCGG GACGCCAGAG GAGGAGCGAC CAATGTGGAG 
AAGAGCTCCG GACTCAGCCC CAGTTTTGTC TTCCTGCAGC TTTATCACTC GCCGTTCTTC 
GGCAATGAGG CCAACAAACC CCTGCTGCTT CCTAAATCAC AGCTGATTGA CCGTGCAGTG 
AAGGTTTTGG ATCAGATGCC TCCGTACGAC ACTCATAAGA TCGGTGTGGT GTTTGTGGGA 
GCCGGACAGG CCAATAATGA GGTGTCGATC CTGTCTAATG AGTACGGCTC GAAGCGCTAT 
GCTCAGTTCC TGACGGGTCT GGGGAAACTC ATCCACTTGA AAGACTGCGA TCCCGATCAG 
ATCTTCCTTG GTGGACTGGA CCAGTACGGA GACGATGGAG AGTTCACATA CTGCTGGCAT 
GATGACATTA TGCAGGCTAT TTTCCACATC GCCACGCTGA TGCCGAACCG TGAGAGCGAC 
CGCGGCTGCT GCAACAAGAA GCGGCACATC GGCAACGACT TCGTGGTGGT GGTTTATAAC 
GACTCGGGGG AGGACTACAA ACTGGGCACC ATCAAGGGTC AGTTCAACTT TGTGGAAGTG 
CTCATAAAGC CGCTGGACTA CGAGAGTAAC CTGGTGACAC TGCAGTGCCG GAAAGATCTG 
GAGGGTTTGG TGGACACCAG TGTAGCGAAG ATCGTCTCTG ACCGGAACCT TCCCCTGCTA 
GTCAGACAGA TGGCGCTTCA TGCTAACATG GCGTCGCTGG TCCACCAGTG CAGGGCGAAC 
CCATCAGACG CATACGCTTC AAAATGGCTG GCCAGACTGA GACACATCAA GAGGATCAGG 
ACGCGGGCTC AGGAGGAGAT TCAGTCCCGC CCCTCTCACG GCATCTCTCT GACTCAAGGC 
CACGCCTCCA TGCAGAATAA ACCCGCCCAC CAGCCGCCGG GAGCAGCACA GAATCCAGAC 
GCCGGCCAGA GGAAGAGGCT GGTGTCCACC GTCGACGACT TCACTGACTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lithium promotes the production of reactive oxygen species via GSK-3?/TSC2/TOR signaling in the gill of zebrafish (Danio rerio).
Chemosphere195854-863(2018 Mar)
Liu D,Gao L,Zhang Z,Tao S,Pang Q,Li A,Deng H,Yu H

mTOR-related neuropathology in mutant tsc2 zebrafish: Phenotypic, transcriptomic and pharmacological analysis.
Neurobiology of disease108225-237(2017 Dec)
Scheldeman C,Mills JD,Siekierska A,Serra I,Copmans D,Iyer AM,Whalley BJ,Maes J,Jansen AC,Lagae L,Aronica E,de Witte PAM

Transcriptomic profiling of mTOR and ryanodine receptor signaling molecules in developing zebrafish in the absence and presence of PCB 95.
PeerJ5e4106(2017)
Frank DF,Miller GW,Connon RE,Geist J,Lein PJ

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tsc2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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