SSH1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SSH1B
Entrez Gene ID	567479
Full Name	slingshot protein phosphatase 1b
Synonyms	fi24e04,si:dkey-202g15.7,wu:fi24e04
General protein information	Preferred Names slingshot protein phosphatase 1b Names protein phosphatase Slingshot homolog 1 slingshot homolog 1b
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171961.4	XP_005172018.1	protein phosphatase Slingshot homolog 1 isoform X2
XM_690775.7	XP_695867.2	protein phosphatase Slingshot homolog 1 isoform X1
XM_021476509.1	XP_021332184.1	protein phosphatase Slingshot homolog 1 isoform X1
XM_021476510.1	XP_021332185.1	protein phosphatase Slingshot homolog 1 isoform X2
XM_690775.6	XP_695867.2	protein phosphatase Slingshot homolog 1 isoform X1
XM_005171961.3	XP_005172018.1	protein phosphatase Slingshot homolog 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SSH1	XP_522524.3
	SSH1	XP_001096246.2
Mus musculus (house mouse)	Ssh1	NP_932777.2
Rattus norvegicus (Norway rat)	Ssh1	XP_006249574.1
Danio rerio (zebrafish)	ssh1b	XP_695867.2
Bos taurus (cattle)	SSH1	XP_002694647.2
Xenopus tropicalis (tropical clawed frog)	ssh1	XP_004910677.1
Homo sapiens (human)	SSH1	NP_061857.3
Gallus gallus (chicken)	SSH1	XP_004945673.1
Danio rerio (zebrafish)	LOC101885659	XP_005165255.1
Canis lupus familiaris (dog)	SSH1	XP_851712.1

Gene Ontology

The following SSH1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSH1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10605	XM_021476509.1 Latest version!	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa10605	XM_690775.7 Latest version!	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa10606	XM_005171961.4 Latest version!	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa10606	XM_021476510.1 Latest version!	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa10605	XM_690775.6	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa10606	XM_005171961.3	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSH1B ( XM_690775.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695867.2 Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10605
Clone ID Related Accession (Same CDS sequence)	XM_690775.7 , XM_021476509.1 , XM_690775.6
Accession Version	XM_690775.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394585.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_690775.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACGACAGT 
GCTAGCATTG GAAACCCCTT CAAAGTGAAG CCCTCATATC AGTCGTGCCG AGACTGCCTG 
CGCCTACCCA GTGGCCGCCG CTGCGAACGT CGCAACCATC GCCTTAACCT GCCACGCCAC 
ACAGGCCTGC CCAGTATCTC CATCCAGGCC CCTGGAGGAC TCAAATTCCG TCCTGTCTCC 
ATCAACAAGG TGCCTCCTCC TGTCCCTCTG CATCACATGA GCAGCGGGCA CTGCCAATGC 
TTTCGCTGCT CCAGTCGGGT CAACTTGGAC ACTTACCGGA AGCAGCGGAG CTGCAAAGAC 
CTTCCTCAGG TTTTGCCTTA TTCCTTGGAG ACTGAGGACA AAATGGAGGG GGAGGACGAG 
GGGAGCAAGG GGAGCTCAAG CAGTCCAACA GCTGAGCTCA CTCTCCTGAA GCTCAGTCTG 
GGTGGTGAGA GGCCTGCGGC GGAGCTCAGT CAGGGGCCAC ACCTAGATGC ACAGCACAGA 
CCGGAGCCAC TGTCCGAACA GATGGATGTG AGCGTAGAGG ACAGTACCAC AGAGGACATG 
GAGGTTGATG AGGGGAACAT CATCCCCTAT GTGCACCCCA GCTCCAGTGA ACTCCAGCAA 
AGGTCTCCAC CAGGCATGTC CCTGGTGCGC AGCTCCAGCA GCGACAGTCT TCAGAGAGTT 
CGAAGAGTTC AGCCGGGCTT GGTACGCCAG CGCACCCGAG AGATTGAGAC CCGTGTGCGG 
CTTGCCGGAC TGACGGTGTC CTCCCAACTC AAAAGGTCTA ACTCGCTGGC CAAGCTAGGG 
GATTTGGCCA TCTCCACAGA GGACCTCTCA CTTTCTGCCA CAGTCTCCAC TGACTATGAT 
GAGCAAGAGC CTGCTCTGTG TGTGCAGTCC TCCTGCTCTC CAAAGCTCTC TTTAACTCTG 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695867.2
CDS	909..3797
Translation	MALVTLQRSPTPSAASSASTTAGEDVGSEDDRKANLSLSESFFMVKGAALFLQQGSSAQEPRTPTHHKNAGDLPQHLQVMINTLRSEDRIKLVVRLESGWTDHVRYMVVVYTNGRQDTEENILLGMDFTDKDSKSCSIGMTLPLWSDTKIHLDGDGGFSVSTAGRLHVFKPVSVQAMWSALQVLHKACEVSRRYNYFPGGMALTWVGYYESCISSEQSCINEWNAMTDLETTRPDSPVMFSDQPTERERTECMIKTKLRNLMMFQDLENITSKEIRNELEQHMSCNLREYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQDSGVGYILNVTREIDNFFPGTFCYCNVRVYDDETTDLLAHWNETYNFIVRAKKNDSKCLVHCKMGVSRSASTVIAYAMKEYGWSLEKAYNFVKQKRNIAQPNPAFMKQLAEYEGILDASKQRHNKLWKPDGDEYSPEGLQASAASGDQTPQFSLLHPDQEERAWGHGGASASPCCGEEPTDHLNYNYYFRRLSDTALDSEPSTPVRGPPLLGMERVFIEIEDVERDALLDDEGFPMAHLGLPGEGTAAQTCGRLEPLEDMRLRLEFSTVEEEDEEEAQKEEAEMAALARGEETPREDESLANSNRFNNENANNSNRLAGKRSCPSGFDDSASIGNPFKVKPSYQSCRDCLRLPSGRRCERRNHRLNLPRHTGLPSISIQAPGGLKFRPVSINKVPPPVPLHHMSSGHCQCFRCSSRVNLDTYRKQRSCKDLPQVLPYSLETEDKMEGEDEGSKGSSSSPTAELTLLKLSLGGERPAAELSQGPHLDAQHRPEPLSEQMDVSVEDSTTEDMEVDEGNIIPYVHPSSSELQQRSPPGMSLVRSSSSDSLQRVRRVQPGLVRQRTREIETRVRLAGLTVSSQLKRSNSLAKLGDLAISTEDLSLSATVSTDYDEQEPALCVQSSCSPKLSLTLKS

Target ORF information:

RefSeq Version	XM_690775.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_690775.7

ORF Insert Sequence:

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACGACAGT 
GCTAGCATTG GAAACCCCTT CAAAGTGAAG CCCTCATATC AGTCGTGCCG AGACTGCCTG 
CGCCTACCCA GTGGCCGCCG CTGCGAACGT CGCAACCATC GCCTTAACCT GCCACGCCAC 
ACAGGCCTGC CCAGTATCTC CATCCAGGCC CCTGGAGGAC TCAAATTCCG TCCTGTCTCC 
ATCAACAAGG TGCCTCCTCC TGTCCCTCTG CATCACATGA GCAGCGGGCA CTGCCAATGC 
TTTCGCTGCT CCAGTCGGGT CAACTTGGAC ACTTACCGGA AGCAGCGGAG CTGCAAAGAC 
CTTCCTCAGG TTTTGCCTTA TTCCTTGGAG ACTGAGGACA AAATGGAGGG GGAGGACGAG 
GGGAGCAAGG GGAGCTCAAG CAGTCCAACA GCTGAGCTCA CTCTCCTGAA GCTCAGTCTG 
GGTGGTGAGA GGCCTGCGGC GGAGCTCAGT CAGGGGCCAC ACCTAGATGC ACAGCACAGA 
CCGGAGCCAC TGTCCGAACA GATGGATGTG AGCGTAGAGG ACAGTACCAC AGAGGACATG 
GAGGTTGATG AGGGGAACAT CATCCCCTAT GTGCACCCCA GCTCCAGTGA ACTCCAGCAA 
AGGTCTCCAC CAGGCATGTC CCTGGTGCGC AGCTCCAGCA GCGACAGTCT TCAGAGAGTT 
CGAAGAGTTC AGCCGGGCTT GGTACGCCAG CGCACCCGAG AGATTGAGAC CCGTGTGCGG 
CTTGCCGGAC TGACGGTGTC CTCCCAACTC AAAAGGTCTA ACTCGCTGGC CAAGCTAGGG 
GATTTGGCCA TCTCCACAGA GGACCTCTCA CTTTCTGCCA CAGTCTCCAC TGACTATGAT 
GAGCAAGAGC CTGCTCTGTG TGTGCAGTCC TCCTGCTCTC CAAAGCTCTC TTTAACTCTG 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSH1B ( XM_021476509.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021332184.1 Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10605
Clone ID Related Accession (Same CDS sequence)	XM_690775.7 , XM_021476509.1 , XM_690775.6
Accession Version	XM_021476509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACGACAGT 
GCTAGCATTG GAAACCCCTT CAAAGTGAAG CCCTCATATC AGTCGTGCCG AGACTGCCTG 
CGCCTACCCA GTGGCCGCCG CTGCGAACGT CGCAACCATC GCCTTAACCT GCCACGCCAC 
ACAGGCCTGC CCAGTATCTC CATCCAGGCC CCTGGAGGAC TCAAATTCCG TCCTGTCTCC 
ATCAACAAGG TGCCTCCTCC TGTCCCTCTG CATCACATGA GCAGCGGGCA CTGCCAATGC 
TTTCGCTGCT CCAGTCGGGT CAACTTGGAC ACTTACCGGA AGCAGCGGAG CTGCAAAGAC 
CTTCCTCAGG TTTTGCCTTA TTCCTTGGAG ACTGAGGACA AAATGGAGGG GGAGGACGAG 
GGGAGCAAGG GGAGCTCAAG CAGTCCAACA GCTGAGCTCA CTCTCCTGAA GCTCAGTCTG 
GGTGGTGAGA GGCCTGCGGC GGAGCTCAGT CAGGGGCCAC ACCTAGATGC ACAGCACAGA 
CCGGAGCCAC TGTCCGAACA GATGGATGTG AGCGTAGAGG ACAGTACCAC AGAGGACATG 
GAGGTTGATG AGGGGAACAT CATCCCCTAT GTGCACCCCA GCTCCAGTGA ACTCCAGCAA 
AGGTCTCCAC CAGGCATGTC CCTGGTGCGC AGCTCCAGCA GCGACAGTCT TCAGAGAGTT 
CGAAGAGTTC AGCCGGGCTT GGTACGCCAG CGCACCCGAG AGATTGAGAC CCGTGTGCGG 
CTTGCCGGAC TGACGGTGTC CTCCCAACTC AAAAGGTCTA ACTCGCTGGC CAAGCTAGGG 
GATTTGGCCA TCTCCACAGA GGACCTCTCA CTTTCTGCCA CAGTCTCCAC TGACTATGAT 
GAGCAAGAGC CTGCTCTGTG TGTGCAGTCC TCCTGCTCTC CAAAGCTCTC TTTAACTCTG 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332184.1
CDS	909..3797
Translation	MALVTLQRSPTPSAASSASTTAGEDVGSEDDRKANLSLSESFFMVKGAALFLQQGSSAQEPRTPTHHKNAGDLPQHLQVMINTLRSEDRIKLVVRLESGWTDHVRYMVVVYTNGRQDTEENILLGMDFTDKDSKSCSIGMTLPLWSDTKIHLDGDGGFSVSTAGRLHVFKPVSVQAMWSALQVLHKACEVSRRYNYFPGGMALTWVGYYESCISSEQSCINEWNAMTDLETTRPDSPVMFSDQPTERERTECMIKTKLRNLMMFQDLENITSKEIRNELEQHMSCNLREYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQDSGVGYILNVTREIDNFFPGTFCYCNVRVYDDETTDLLAHWNETYNFIVRAKKNDSKCLVHCKMGVSRSASTVIAYAMKEYGWSLEKAYNFVKQKRNIAQPNPAFMKQLAEYEGILDASKQRHNKLWKPDGDEYSPEGLQASAASGDQTPQFSLLHPDQEERAWGHGGASASPCCGEEPTDHLNYNYYFRRLSDTALDSEPSTPVRGPPLLGMERVFIEIEDVERDALLDDEGFPMAHLGLPGEGTAAQTCGRLEPLEDMRLRLEFSTVEEEDEEEAQKEEAEMAALARGEETPREDESLANSNRFNNENANNSNRLAGKRSCPSGFDDSASIGNPFKVKPSYQSCRDCLRLPSGRRCERRNHRLNLPRHTGLPSISIQAPGGLKFRPVSINKVPPPVPLHHMSSGHCQCFRCSSRVNLDTYRKQRSCKDLPQVLPYSLETEDKMEGEDEGSKGSSSSPTAELTLLKLSLGGERPAAELSQGPHLDAQHRPEPLSEQMDVSVEDSTTEDMEVDEGNIIPYVHPSSSELQQRSPPGMSLVRSSSSDSLQRVRRVQPGLVRQRTREIETRVRLAGLTVSSQLKRSNSLAKLGDLAISTEDLSLSATVSTDYDEQEPALCVQSSCSPKLSLTLKS

Target ORF information:

RefSeq Version	XM_021476509.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476509.1

ORF Insert Sequence:

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACGACAGT 
GCTAGCATTG GAAACCCCTT CAAAGTGAAG CCCTCATATC AGTCGTGCCG AGACTGCCTG 
CGCCTACCCA GTGGCCGCCG CTGCGAACGT CGCAACCATC GCCTTAACCT GCCACGCCAC 
ACAGGCCTGC CCAGTATCTC CATCCAGGCC CCTGGAGGAC TCAAATTCCG TCCTGTCTCC 
ATCAACAAGG TGCCTCCTCC TGTCCCTCTG CATCACATGA GCAGCGGGCA CTGCCAATGC 
TTTCGCTGCT CCAGTCGGGT CAACTTGGAC ACTTACCGGA AGCAGCGGAG CTGCAAAGAC 
CTTCCTCAGG TTTTGCCTTA TTCCTTGGAG ACTGAGGACA AAATGGAGGG GGAGGACGAG 
GGGAGCAAGG GGAGCTCAAG CAGTCCAACA GCTGAGCTCA CTCTCCTGAA GCTCAGTCTG 
GGTGGTGAGA GGCCTGCGGC GGAGCTCAGT CAGGGGCCAC ACCTAGATGC ACAGCACAGA 
CCGGAGCCAC TGTCCGAACA GATGGATGTG AGCGTAGAGG ACAGTACCAC AGAGGACATG 
GAGGTTGATG AGGGGAACAT CATCCCCTAT GTGCACCCCA GCTCCAGTGA ACTCCAGCAA 
AGGTCTCCAC CAGGCATGTC CCTGGTGCGC AGCTCCAGCA GCGACAGTCT TCAGAGAGTT 
CGAAGAGTTC AGCCGGGCTT GGTACGCCAG CGCACCCGAG AGATTGAGAC CCGTGTGCGG 
CTTGCCGGAC TGACGGTGTC CTCCCAACTC AAAAGGTCTA ACTCGCTGGC CAAGCTAGGG 
GATTTGGCCA TCTCCACAGA GGACCTCTCA CTTTCTGCCA CAGTCTCCAC TGACTATGAT 
GAGCAAGAGC CTGCTCTGTG TGTGCAGTCC TCCTGCTCTC CAAAGCTCTC TTTAACTCTG 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSH1B ( XM_690775.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695867.2 Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10605
Clone ID Related Accession (Same CDS sequence)	XM_690775.7 , XM_021476509.1 , XM_690775.6
Accession Version	XM_690775.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_690775.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACGACAGT 
GCTAGCATTG GAAACCCCTT CAAAGTGAAG CCCTCATATC AGTCGTGCCG AGACTGCCTG 
CGCCTACCCA GTGGCCGCCG CTGCGAACGT CGCAACCATC GCCTTAACCT GCCACGCCAC 
ACAGGCCTGC CCAGTATCTC CATCCAGGCC CCTGGAGGAC TCAAATTCCG TCCTGTCTCC 
ATCAACAAGG TGCCTCCTCC TGTCCCTCTG CATCACATGA GCAGCGGGCA CTGCCAATGC 
TTTCGCTGCT CCAGTCGGGT CAACTTGGAC ACTTACCGGA AGCAGCGGAG CTGCAAAGAC 
CTTCCTCAGG TTTTGCCTTA TTCCTTGGAG ACTGAGGACA AAATGGAGGG GGAGGACGAG 
GGGAGCAAGG GGAGCTCAAG CAGTCCAACA GCTGAGCTCA CTCTCCTGAA GCTCAGTCTG 
GGTGGTGAGA GGCCTGCGGC GGAGCTCAGT CAGGGGCCAC ACCTAGATGC ACAGCACAGA 
CCGGAGCCAC TGTCCGAACA GATGGATGTG AGCGTAGAGG ACAGTACCAC AGAGGACATG 
GAGGTTGATG AGGGGAACAT CATCCCCTAT GTGCACCCCA GCTCCAGTGA ACTCCAGCAA 
AGGTCTCCAC CAGGCATGTC CCTGGTGCGC AGCTCCAGCA GCGACAGTCT TCAGAGAGTT 
CGAAGAGTTC AGCCGGGCTT GGTACGCCAG CGCACCCGAG AGATTGAGAC CCGTGTGCGG 
CTTGCCGGAC TGACGGTGTC CTCCCAACTC AAAAGGTCTA ACTCGCTGGC CAAGCTAGGG 
GATTTGGCCA TCTCCACAGA GGACCTCTCA CTTTCTGCCA CAGTCTCCAC TGACTATGAT 
GAGCAAGAGC CTGCTCTGTG TGTGCAGTCC TCCTGCTCTC CAAAGCTCTC TTTAACTCTG 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695867.2
CDS	1045..3933
Translation	MALVTLQRSPTPSAASSASTTAGEDVGSEDDRKANLSLSESFFMVKGAALFLQQGSSAQEPRTPTHHKNAGDLPQHLQVMINTLRSEDRIKLVVRLESGWTDHVRYMVVVYTNGRQDTEENILLGMDFTDKDSKSCSIGMTLPLWSDTKIHLDGDGGFSVSTAGRLHVFKPVSVQAMWSALQVLHKACEVSRRYNYFPGGMALTWVGYYESCISSEQSCINEWNAMTDLETTRPDSPVMFSDQPTERERTECMIKTKLRNLMMFQDLENITSKEIRNELEQHMSCNLREYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQDSGVGYILNVTREIDNFFPGTFCYCNVRVYDDETTDLLAHWNETYNFIVRAKKNDSKCLVHCKMGVSRSASTVIAYAMKEYGWSLEKAYNFVKQKRNIAQPNPAFMKQLAEYEGILDASKQRHNKLWKPDGDEYSPEGLQASAASGDQTPQFSLLHPDQEERAWGHGGASASPCCGEEPTDHLNYNYYFRRLSDTALDSEPSTPVRGPPLLGMERVFIEIEDVERDALLDDEGFPMAHLGLPGEGTAAQTCGRLEPLEDMRLRLEFSTVEEEDEEEAQKEEAEMAALARGEETPREDESLANSNRFNNENANNSNRLAGKRSCPSGFDDSASIGNPFKVKPSYQSCRDCLRLPSGRRCERRNHRLNLPRHTGLPSISIQAPGGLKFRPVSINKVPPPVPLHHMSSGHCQCFRCSSRVNLDTYRKQRSCKDLPQVLPYSLETEDKMEGEDEGSKGSSSSPTAELTLLKLSLGGERPAAELSQGPHLDAQHRPEPLSEQMDVSVEDSTTEDMEVDEGNIIPYVHPSSSELQQRSPPGMSLVRSSSSDSLQRVRRVQPGLVRQRTREIETRVRLAGLTVSSQLKRSNSLAKLGDLAISTEDLSLSATVSTDYDEQEPALCVQSSCSPKLSLTLKS

Target ORF information:

RefSeq Version	XM_690775.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_690775.6

ORF Insert Sequence:

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACGACAGT 
GCTAGCATTG GAAACCCCTT CAAAGTGAAG CCCTCATATC AGTCGTGCCG AGACTGCCTG 
CGCCTACCCA GTGGCCGCCG CTGCGAACGT CGCAACCATC GCCTTAACCT GCCACGCCAC 
ACAGGCCTGC CCAGTATCTC CATCCAGGCC CCTGGAGGAC TCAAATTCCG TCCTGTCTCC 
ATCAACAAGG TGCCTCCTCC TGTCCCTCTG CATCACATGA GCAGCGGGCA CTGCCAATGC 
TTTCGCTGCT CCAGTCGGGT CAACTTGGAC ACTTACCGGA AGCAGCGGAG CTGCAAAGAC 
CTTCCTCAGG TTTTGCCTTA TTCCTTGGAG ACTGAGGACA AAATGGAGGG GGAGGACGAG 
GGGAGCAAGG GGAGCTCAAG CAGTCCAACA GCTGAGCTCA CTCTCCTGAA GCTCAGTCTG 
GGTGGTGAGA GGCCTGCGGC GGAGCTCAGT CAGGGGCCAC ACCTAGATGC ACAGCACAGA 
CCGGAGCCAC TGTCCGAACA GATGGATGTG AGCGTAGAGG ACAGTACCAC AGAGGACATG 
GAGGTTGATG AGGGGAACAT CATCCCCTAT GTGCACCCCA GCTCCAGTGA ACTCCAGCAA 
AGGTCTCCAC CAGGCATGTC CCTGGTGCGC AGCTCCAGCA GCGACAGTCT TCAGAGAGTT 
CGAAGAGTTC AGCCGGGCTT GGTACGCCAG CGCACCCGAG AGATTGAGAC CCGTGTGCGG 
CTTGCCGGAC TGACGGTGTC CTCCCAACTC AAAAGGTCTA ACTCGCTGGC CAAGCTAGGG 
GATTTGGCCA TCTCCACAGA GGACCTCTCA CTTTCTGCCA CAGTCTCCAC TGACTATGAT 
GAGCAAGAGC CTGCTCTGTG TGTGCAGTCC TCCTGCTCTC CAAAGCTCTC TTTAACTCTG 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSH1B ( XM_005171961.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172018.1 Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10606
Clone ID Related Accession (Same CDS sequence)	XM_005171961.4 , XM_021476510.1 , XM_005171961.3
Accession Version	XM_005171961.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394585.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171961.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172018.1
CDS	907..2886
Translation	MALVTLQRSPTPSAASSASTTAGEDVGSEDDRKANLSLSESFFMVKGAALFLQQGSSAQEPRTPTHHKNAGDLPQHLQVMINTLRSEDRIKLVVRLESGWTDHVRYMVVVYTNGRQDTEENILLGMDFTDKDSKSCSIGMTLPLWSDTKIHLDGDGGFSVSTAGRLHVFKPVSVQAMWSALQVLHKACEVSRRYNYFPGGMALTWVGYYESCISSEQSCINEWNAMTDLETTRPDSPVMFSDQPTERERTECMIKTKLRNLMMFQDLENITSKEIRNELEQHMSCNLREYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQDSGVGYILNVTREIDNFFPGTFCYCNVRVYDDETTDLLAHWNETYNFIVRAKKNDSKCLVHCKMGVSRSASTVIAYAMKEYGWSLEKAYNFVKQKRNIAQPNPAFMKQLAEYEGILDASKQRHNKLWKPDGDEYSPEGLQASAASGDQTPQFSLLHPDQEERAWGHGGASASPCCGEEPTDHLNYNYYFRRLSDTALDSEPSTPVRGPPLLGMERVFIEIEDVERDALLDDEGFPMAHLGLPGEGTAAQTCGRLEPLEDMRLRLEFSTVEEEDEEEAQKEEAEMAALARGEETPREDESLANSNRFNNENANNSNRLAGKRSCPSGFDC

Target ORF information:

RefSeq Version	XM_005171961.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171961.4

ORF Insert Sequence:

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSH1B ( XM_021476510.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021332185.1 Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.

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CloneID	ODa10606
Clone ID Related Accession (Same CDS sequence)	XM_005171961.4 , XM_021476510.1 , XM_005171961.3
Accession Version	XM_021476510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332185.1
CDS	907..2886
Translation	MALVTLQRSPTPSAASSASTTAGEDVGSEDDRKANLSLSESFFMVKGAALFLQQGSSAQEPRTPTHHKNAGDLPQHLQVMINTLRSEDRIKLVVRLESGWTDHVRYMVVVYTNGRQDTEENILLGMDFTDKDSKSCSIGMTLPLWSDTKIHLDGDGGFSVSTAGRLHVFKPVSVQAMWSALQVLHKACEVSRRYNYFPGGMALTWVGYYESCISSEQSCINEWNAMTDLETTRPDSPVMFSDQPTERERTECMIKTKLRNLMMFQDLENITSKEIRNELEQHMSCNLREYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQDSGVGYILNVTREIDNFFPGTFCYCNVRVYDDETTDLLAHWNETYNFIVRAKKNDSKCLVHCKMGVSRSASTVIAYAMKEYGWSLEKAYNFVKQKRNIAQPNPAFMKQLAEYEGILDASKQRHNKLWKPDGDEYSPEGLQASAASGDQTPQFSLLHPDQEERAWGHGGASASPCCGEEPTDHLNYNYYFRRLSDTALDSEPSTPVRGPPLLGMERVFIEIEDVERDALLDDEGFPMAHLGLPGEGTAAQTCGRLEPLEDMRLRLEFSTVEEEDEEEAQKEEAEMAALARGEETPREDESLANSNRFNNENANNSNRLAGKRSCPSGFDC

Target ORF information:

RefSeq Version	XM_021476510.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476510.1

ORF Insert Sequence:

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSH1B ( XM_005171961.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172018.1 Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.

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CloneID	ODa10606
Clone ID Related Accession (Same CDS sequence)	XM_005171961.4 , XM_021476510.1 , XM_005171961.3
Accession Version	XM_005171961.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172018.1
CDS	1048..3027
Translation	MALVTLQRSPTPSAASSASTTAGEDVGSEDDRKANLSLSESFFMVKGAALFLQQGSSAQEPRTPTHHKNAGDLPQHLQVMINTLRSEDRIKLVVRLESGWTDHVRYMVVVYTNGRQDTEENILLGMDFTDKDSKSCSIGMTLPLWSDTKIHLDGDGGFSVSTAGRLHVFKPVSVQAMWSALQVLHKACEVSRRYNYFPGGMALTWVGYYESCISSEQSCINEWNAMTDLETTRPDSPVMFSDQPTERERTECMIKTKLRNLMMFQDLENITSKEIRNELEQHMSCNLREYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQDSGVGYILNVTREIDNFFPGTFCYCNVRVYDDETTDLLAHWNETYNFIVRAKKNDSKCLVHCKMGVSRSASTVIAYAMKEYGWSLEKAYNFVKQKRNIAQPNPAFMKQLAEYEGILDASKQRHNKLWKPDGDEYSPEGLQASAASGDQTPQFSLLHPDQEERAWGHGGASASPCCGEEPTDHLNYNYYFRRLSDTALDSEPSTPVRGPPLLGMERVFIEIEDVERDALLDDEGFPMAHLGLPGEGTAAQTCGRLEPLEDMRLRLEFSTVEEEDEEEAQKEEAEMAALARGEETPREDESLANSNRFNNENANNSNRLAGKRSCPSGFDC

Target ORF information:

RefSeq Version	XM_005171961.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1b (ssh1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171961.3

ORF Insert Sequence:

ATGGCTCTGG TGACTCTGCA GCGCTCCCCG ACACCCAGCG CCGCATCCTC AGCCAGCACC 
ACCGCGGGGG AGGATGTCGG CAGTGAAGAT GATCGAAAGG CAAACCTAAG CCTGAGTGAA 
AGTTTCTTCA TGGTAAAAGG GGCAGCTCTG TTTTTACAAC AAGGCAGCAG TGCACAAGAG 
CCCAGAACAC CCACACATCA TAAAAACGCA GGGGATCTTC CACAGCATCT ACAAGTAATG 
ATAAACACCC TCCGCTCTGA AGATAGAATT AAATTAGTCG TTAGGTTAGA AAGTGGATGG 
ACGGACCATG TGCGGTACAT GGTAGTTGTC TACACAAACG GCCGCCAAGA CACTGAAGAA 
AACATTCTAC TCGGGATGGA CTTCACGGAT AAGGATAGTA AAAGTTGCTC CATTGGGATG 
ACTCTTCCGC TTTGGAGCGA TACCAAGATT CATCTGGATG GAGATGGGGG TTTCAGCGTG 
AGCACAGCAG GAAGACTACA TGTTTTTAAG CCTGTGTCAG TGCAGGCCAT GTGGTCCGCA 
CTGCAGGTGT TGCACAAAGC ATGCGAGGTG TCCCGCAGGT ACAACTATTT TCCAGGAGGA 
ATGGCACTGA CGTGGGTGGG CTATTACGAG AGCTGTATCT CCTCAGAGCA GAGCTGTATC 
AATGAGTGGA ATGCAATGAC AGATCTGGAG ACGACCCGGC CTGATTCTCC AGTCATGTTT 
TCTGATCAGC CAACAGAGAG AGAAAGAACA GAGTGTATGA TCAAAACCAA ACTCCGAAAC 
CTAATGATGT TCCAGGACCT GGAGAATATT ACTTCTAAGG AGATCCGAAA TGAGCTGGAA 
CAACATATGA GCTGCAACCT GAGGGAGTAT AAAGAGTTTA TTGACAATGA GATGCTGCTT 
ATTCTGGGCC AGATGGACAA GGCTACGCTC ATATTTGACC ATCTGTACCT GGGCTCAGAG 
TGGAATGCAT CCAACTTGGA GGAGCTTCAA GACTCTGGGG TTGGGTATAT CCTCAATGTC 
ACAAGAGAGA TTGACAACTT TTTCCCAGGT ACCTTTTGTT ATTGTAATGT CCGTGTTTAT 
GATGATGAGA CTACAGATCT GCTGGCGCAC TGGAACGAAA CCTACAACTT CATAGTTAGA 
GCCAAGAAAA ACGACTCTAA ATGCCTAGTA CACTGCAAGA TGGGAGTGAG TCGCTCAGCA 
TCTACTGTCA TTGCATATGC CATGAAGGAG TATGGATGGT CACTTGAGAA GGCTTACAAT 
TTTGTCAAAC AGAAGAGGAA TATAGCACAA CCAAATCCAG CATTTATGAA ACAGCTGGCC 
GAGTACGAGG GGATTTTAGA TGCCAGCAAG CAGAGACACA ATAAACTCTG GAAACCAGAT 
GGGGATGAGT ATTCACCAGA GGGTCTCCAG GCTTCTGCTG CCAGTGGTGA TCAGACTCCT 
CAGTTTTCAT TGCTGCACCC TGATCAGGAG GAGAGGGCTT GGGGCCACGG AGGAGCAAGT 
GCTTCCCCCT GCTGTGGAGA AGAGCCCACA GACCATCTCA ATTACAACTA CTACTTCCGG 
CGTCTCTCAG ACACCGCTCT GGACAGCGAA CCCTCCACGC CGGTCCGGGG TCCTCCACTT 
TTAGGCATGG AGAGGGTCTT TATTGAGATC GAAGACGTGG AGAGGGACGC GTTGCTTGAC 
GATGAAGGTT TCCCCATGGC TCACTTGGGC CTTCCTGGGG AAGGCACAGC AGCTCAGACC 
TGTGGCCGAC TGGAGCCTTT GGAGGACATG CGCTTGCGGC TGGAGTTTAG CACGGTGGAG 
GAGGAAGATG AGGAGGAAGC ACAAAAGGAG GAGGCGGAGA TGGCGGCCTT AGCTCGCGGC 
GAGGAGACGC CGAGGGAAGA CGAGAGCCTG GCTAATTCGA ACCGCTTTAA CAACGAGAAC 
GCCAACAACA GCAACAGGCT GGCTGGGAAG CGCAGCTGCC CTTCTGGCTT TGACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SSH1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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