si:dkey-151j17.4 cDNA ORF clone, Danio rerio(zebrafish)

The following si:dkey-151j17.4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:dkey-151j17.4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa23655 XM_005173698.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23656 XM_005173699.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23657 XM_005173700.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23658 XM_005173701.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23659 XM_005173702.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23660 XM_005173703.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23661 XM_005173704.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23662 XM_005173705.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23663 XM_005173706.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23664 XM_005173707.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23665 XM_005173708.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23666 XM_005173709.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23667 XM_005173710.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23668 XM_005173711.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23669 XM_005173712.4
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45083 XM_009293898.3
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa56012 XM_017352155.2
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa56013 XM_017352156.2
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa56014 XM_017352157.2
Latest version!
Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
ODa56014 XM_017352157.1 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23659 XM_005173702.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23656 XM_005173699.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23669 XM_005173712.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23655 XM_005173698.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23660 XM_005173703.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23666 XM_005173709.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23661 XM_005173704.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa56013 XM_017352156.1 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23658 XM_005173701.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23664 XM_005173707.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23668 XM_005173711.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45083 XM_009293898.2 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23662 XM_005173705.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23667 XM_005173710.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23665 XM_005173708.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa23657 XM_005173700.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa56012 XM_017352155.1 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa23663 XM_005173706.3 Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa23655
    Clone ID Related Accession (Same CDS sequence) XM_005173698.3 , XM_005173698.4
    Accession Version XM_005173698.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    601
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    721
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
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    3421
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    4141
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    4681
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    7141
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    7741
    7801
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTCAACCCC CAAATGGGCC AGAAGGTGGT CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTGAGGAA AGTGTCTCAG ACAACCCTTT TACAAAGGGA
    CAAGATTTTG AGATGCTCAC CGTCCTCCAG CCAGAAGGAT ATCAAGTGTA TGTGAACGGC
    AAGGTGCTGC ACACATTCAA GCACCGTATG CAACTAGAAA AAGTGTCCAC CTTAAATATA
    TTTGGAGGTG TTGCAGTAAA TCTTTTGGGC TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    GCTACCCAAG TCAAGTCAAC ATACACCAGT CTGGGCAGCT CACATCTGGA GCAATCAACC
    ACACACTCAG AGATCGTGCA AGCAGTCCAA AACCCTAACC TTCCTTATGT GGGCTCAATT
    TCTGGAGGAC TGAGAGAAGG CATGGCCTTG TACTTCCAGG GTGTTGTTCC TCCTAATGCT
    GACAAATTTG AGATCAATTT CAAGACTGGG CAGTCTGGCA GTGATGACAT TGCTTTCCAC
    TTTAACCCCC TAATGGGCCA GAATTTGGAC ATGAACAGCT TCAGGAATGG AGCATGGGAA
    ACTCAGGAGA GTGTCTCCAA CAACCCTTTT AAAAAGGGGG AAGCTTTTGA GATGTTCATG
    GTCATCAAAT CTGAAGGATA TCAGGTATAT GTAAACGAAA ACGACCTGTA CACATTCAAG
    CACCGTATAC CACTGGAAAA AGTGTCCATC TTGAATATTG TTGGAGCGGT TTCTATGAAC
    ATTTTTGGGT TCATTCAAAA CTGGAGCAAA TCCTCATTTA CTACTAGAGT CAAGTCAACA
    ATCACCAGTC TGGGAAGCCC ACAGTGGGAT CTGTCCATAA TACAATCAAA GGCCTTACAA
    GCAGTGCAAA ATCCCAACCT TCCTTATTTG GGCCAAATCC CTGGAGGACT AAGAGAAGGC
    ATGACCTTGT ATGTGAAGGG AGTTGTTCCT AGTAATGGTG ATCGGTTTTC AATCAACTTC
    AAGACCGGGT CTACTGATAA GGATGACATT GCTTTCCACT TCAATCCCCG AATGGGCTCA
    AAGCTGGTCA TGAACAGTAT GAAGAGCGGG AGATGGGGAG CGGAGGAATA CGTCTCTGAA
    AACCCGTGTA AAAAGGGAGA TGCTTTTGAG TTCTACATTC AGATCAAAGC TGAAGGATAT
    CAGGTGCAAT TCAAAGACCA CAAGCAGAGC ATGTTCAAAC ACCGCATCCC TTTTGAAAGA
    GTGACTACAA TCAACGTTCT GGGGAATGTC TCTATCAGCT TTCTTGGATT TGTCATGGGG
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173755.1
    CDS66..7871
    Translation

    Target ORF information:

    RefSeq Version XM_005173698.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173698.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTCAACCCC CAAATGGGCC AGAAGGTGGT CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTGAGGAA AGTGTCTCAG ACAACCCTTT TACAAAGGGA
    CAAGATTTTG AGATGCTCAC CGTCCTCCAG CCAGAAGGAT ATCAAGTGTA TGTGAACGGC
    AAGGTGCTGC ACACATTCAA GCACCGTATG CAACTAGAAA AAGTGTCCAC CTTAAATATA
    TTTGGAGGTG TTGCAGTAAA TCTTTTGGGC TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    GCTACCCAAG TCAAGTCAAC ATACACCAGT CTGGGCAGCT CACATCTGGA GCAATCAACC
    ACACACTCAG AGATCGTGCA AGCAGTCCAA AACCCTAACC TTCCTTATGT GGGCTCAATT
    TCTGGAGGAC TGAGAGAAGG CATGGCCTTG TACTTCCAGG GTGTTGTTCC TCCTAATGCT
    GACAAATTTG AGATCAATTT CAAGACTGGG CAGTCTGGCA GTGATGACAT TGCTTTCCAC
    TTTAACCCCC TAATGGGCCA GAATTTGGAC ATGAACAGCT TCAGGAATGG AGCATGGGAA
    ACTCAGGAGA GTGTCTCCAA CAACCCTTTT AAAAAGGGGG AAGCTTTTGA GATGTTCATG
    GTCATCAAAT CTGAAGGATA TCAGGTATAT GTAAACGAAA ACGACCTGTA CACATTCAAG
    CACCGTATAC CACTGGAAAA AGTGTCCATC TTGAATATTG TTGGAGCGGT TTCTATGAAC
    ATTTTTGGGT TCATTCAAAA CTGGAGCAAA TCCTCATTTA CTACTAGAGT CAAGTCAACA
    ATCACCAGTC TGGGAAGCCC ACAGTGGGAT CTGTCCATAA TACAATCAAA GGCCTTACAA
    GCAGTGCAAA ATCCCAACCT TCCTTATTTG GGCCAAATCC CTGGAGGACT AAGAGAAGGC
    ATGACCTTGT ATGTGAAGGG AGTTGTTCCT AGTAATGGTG ATCGGTTTTC AATCAACTTC
    AAGACCGGGT CTACTGATAA GGATGACATT GCTTTCCACT TCAATCCCCG AATGGGCTCA
    AAGCTGGTCA TGAACAGTAT GAAGAGCGGG AGATGGGGAG CGGAGGAATA CGTCTCTGAA
    AACCCGTGTA AAAAGGGAGA TGCTTTTGAG TTCTACATTC AGATCAAAGC TGAAGGATAT
    CAGGTGCAAT TCAAAGACCA CAAGCAGAGC ATGTTCAAAC ACCGCATCCC TTTTGAAAGA
    GTGACTACAA TCAACGTTCT GGGGAATGTC TCTATCAGCT TTCTTGGATT TGTCATGGGG
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23655
    Clone ID Related Accession (Same CDS sequence) XM_005173698.3 , XM_005173698.4
    Accession Version XM_005173698.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173698.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTCAACCCC CAAATGGGCC AGAAGGTGGT CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTGAGGAA AGTGTCTCAG ACAACCCTTT TACAAAGGGA
    CAAGATTTTG AGATGCTCAC CGTCCTCCAG CCAGAAGGAT ATCAAGTGTA TGTGAACGGC
    AAGGTGCTGC ACACATTCAA GCACCGTATG CAACTAGAAA AAGTGTCCAC CTTAAATATA
    TTTGGAGGTG TTGCAGTAAA TCTTTTGGGC TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    GCTACCCAAG TCAAGTCAAC ATACACCAGT CTGGGCAGCT CACATCTGGA GCAATCAACC
    ACACACTCAG AGATCGTGCA AGCAGTCCAA AACCCTAACC TTCCTTATGT GGGCTCAATT
    TCTGGAGGAC TGAGAGAAGG CATGGCCTTG TACTTCCAGG GTGTTGTTCC TCCTAATGCT
    GACAAATTTG AGATCAATTT CAAGACTGGG CAGTCTGGCA GTGATGACAT TGCTTTCCAC
    TTTAACCCCC TAATGGGCCA GAATTTGGAC ATGAACAGCT TCAGGAATGG AGCATGGGAA
    ACTCAGGAGA GTGTCTCCAA CAACCCTTTT AAAAAGGGGG AAGCTTTTGA GATGTTCATG
    GTCATCAAAT CTGAAGGATA TCAGGTATAT GTAAACGAAA ACGACCTGTA CACATTCAAG
    CACCGTATAC CACTGGAAAA AGTGTCCATC TTGAATATTG TTGGAGCGGT TTCTATGAAC
    ATTTTTGGGT TCATTCAAAA CTGGAGCAAA TCCTCATTTA CTACTAGAGT CAAGTCAACA
    ATCACCAGTC TGGGAAGCCC ACAGTGGGAT CTGTCCATAA TACAATCAAA GGCCTTACAA
    GCAGTGCAAA ATCCCAACCT TCCTTATTTG GGCCAAATCC CTGGAGGACT AAGAGAAGGC
    ATGACCTTGT ATGTGAAGGG AGTTGTTCCT AGTAATGGTG ATCGGTTTTC AATCAACTTC
    AAGACCGGGT CTACTGATAA GGATGACATT GCTTTCCACT TCAATCCCCG AATGGGCTCA
    AAGCTGGTCA TGAACAGTAT GAAGAGCGGG AGATGGGGAG CGGAGGAATA CGTCTCTGAA
    AACCCGTGTA AAAAGGGAGA TGCTTTTGAG TTCTACATTC AGATCAAAGC TGAAGGATAT
    CAGGTGCAAT TCAAAGACCA CAAGCAGAGC ATGTTCAAAC ACCGCATCCC TTTTGAAAGA
    GTGACTACAA TCAACGTTCT GGGGAATGTC TCTATCAGCT TTCTTGGATT TGTCATGGGG
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173755.1
    CDS315..8120
    Translation

    Target ORF information:

    RefSeq Version XM_005173698.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173698.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTCAACCCC CAAATGGGCC AGAAGGTGGT CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTGAGGAA AGTGTCTCAG ACAACCCTTT TACAAAGGGA
    CAAGATTTTG AGATGCTCAC CGTCCTCCAG CCAGAAGGAT ATCAAGTGTA TGTGAACGGC
    AAGGTGCTGC ACACATTCAA GCACCGTATG CAACTAGAAA AAGTGTCCAC CTTAAATATA
    TTTGGAGGTG TTGCAGTAAA TCTTTTGGGC TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    GCTACCCAAG TCAAGTCAAC ATACACCAGT CTGGGCAGCT CACATCTGGA GCAATCAACC
    ACACACTCAG AGATCGTGCA AGCAGTCCAA AACCCTAACC TTCCTTATGT GGGCTCAATT
    TCTGGAGGAC TGAGAGAAGG CATGGCCTTG TACTTCCAGG GTGTTGTTCC TCCTAATGCT
    GACAAATTTG AGATCAATTT CAAGACTGGG CAGTCTGGCA GTGATGACAT TGCTTTCCAC
    TTTAACCCCC TAATGGGCCA GAATTTGGAC ATGAACAGCT TCAGGAATGG AGCATGGGAA
    ACTCAGGAGA GTGTCTCCAA CAACCCTTTT AAAAAGGGGG AAGCTTTTGA GATGTTCATG
    GTCATCAAAT CTGAAGGATA TCAGGTATAT GTAAACGAAA ACGACCTGTA CACATTCAAG
    CACCGTATAC CACTGGAAAA AGTGTCCATC TTGAATATTG TTGGAGCGGT TTCTATGAAC
    ATTTTTGGGT TCATTCAAAA CTGGAGCAAA TCCTCATTTA CTACTAGAGT CAAGTCAACA
    ATCACCAGTC TGGGAAGCCC ACAGTGGGAT CTGTCCATAA TACAATCAAA GGCCTTACAA
    GCAGTGCAAA ATCCCAACCT TCCTTATTTG GGCCAAATCC CTGGAGGACT AAGAGAAGGC
    ATGACCTTGT ATGTGAAGGG AGTTGTTCCT AGTAATGGTG ATCGGTTTTC AATCAACTTC
    AAGACCGGGT CTACTGATAA GGATGACATT GCTTTCCACT TCAATCCCCG AATGGGCTCA
    AAGCTGGTCA TGAACAGTAT GAAGAGCGGG AGATGGGGAG CGGAGGAATA CGTCTCTGAA
    AACCCGTGTA AAAAGGGAGA TGCTTTTGAG TTCTACATTC AGATCAAAGC TGAAGGATAT
    CAGGTGCAAT TCAAAGACCA CAAGCAGAGC ATGTTCAAAC ACCGCATCCC TTTTGAAAGA
    GTGACTACAA TCAACGTTCT GGGGAATGTC TCTATCAGCT TTCTTGGATT TGTCATGGGG
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23656
    Clone ID Related Accession (Same CDS sequence) XM_005173699.4 , XM_005173699.3
    Accession Version XM_005173699.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173699.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173756.1
    CDS315..7601
    Translation

    Target ORF information:

    RefSeq Version XM_005173699.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173699.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23656
    Clone ID Related Accession (Same CDS sequence) XM_005173699.4 , XM_005173699.3
    Accession Version XM_005173699.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173756.1
    CDS66..7352
    Translation

    Target ORF information:

    RefSeq Version XM_005173699.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173699.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23657
    Clone ID Related Accession (Same CDS sequence) XM_005173700.3 , XM_005173700.4
    Accession Version XM_005173700.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173700.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TGAGATCAAT
    TTCAAGACTG GGCAGTCTGG CAGTGATGAC ATTGCTTTCC ACTTCAACCC CCAAATGGGC
    CAGAAGGTGG TCATGAACAG CTTCAGGAAT GGAGCATGGG AAACTGAGGA AAGTGTCTCA
    GACAACCCTT TTACAAAGGG ACAAGATTTT GAGATGCTCA CCGTCCTCCA GCCAGAAGGA
    TATCAAGTGT ATGTGAACGG CAAGGTGCTG CACACATTCA AGCACCGTAT GCAACTAGAA
    AAAGTGTCCA CCTTAAATAT ATTTGGAGGT GTTGCAGTAA ATCTTTTGGG CTTCATTCAA
    AACTGGAGCA AATCCTCATT TGCTACCCAA GTCAAGTCAA CATACACCAG TCTGGGCAGC
    TCACATCTGG AGCAATCAAC CACACACTCA GAGATCGTGC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173757.1
    CDS66..7352
    Translation

    Target ORF information:

    RefSeq Version XM_005173700.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173700.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TGAGATCAAT
    TTCAAGACTG GGCAGTCTGG CAGTGATGAC ATTGCTTTCC ACTTCAACCC CCAAATGGGC
    CAGAAGGTGG TCATGAACAG CTTCAGGAAT GGAGCATGGG AAACTGAGGA AAGTGTCTCA
    GACAACCCTT TTACAAAGGG ACAAGATTTT GAGATGCTCA CCGTCCTCCA GCCAGAAGGA
    TATCAAGTGT ATGTGAACGG CAAGGTGCTG CACACATTCA AGCACCGTAT GCAACTAGAA
    AAAGTGTCCA CCTTAAATAT ATTTGGAGGT GTTGCAGTAA ATCTTTTGGG CTTCATTCAA
    AACTGGAGCA AATCCTCATT TGCTACCCAA GTCAAGTCAA CATACACCAG TCTGGGCAGC
    TCACATCTGG AGCAATCAAC CACACACTCA GAGATCGTGC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23657
    Clone ID Related Accession (Same CDS sequence) XM_005173700.3 , XM_005173700.4
    Accession Version XM_005173700.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173700.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1981
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    2641
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    3181
    3241
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    3421
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    3961
    4021
    4081
    4141
    4201
    4261
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    4381
    4441
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    4621
    4681
    4741
    4801
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    4981
    5041
    5101
    5161
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    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
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    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TGAGATCAAT
    TTCAAGACTG GGCAGTCTGG CAGTGATGAC ATTGCTTTCC ACTTCAACCC CCAAATGGGC
    CAGAAGGTGG TCATGAACAG CTTCAGGAAT GGAGCATGGG AAACTGAGGA AAGTGTCTCA
    GACAACCCTT TTACAAAGGG ACAAGATTTT GAGATGCTCA CCGTCCTCCA GCCAGAAGGA
    TATCAAGTGT ATGTGAACGG CAAGGTGCTG CACACATTCA AGCACCGTAT GCAACTAGAA
    AAAGTGTCCA CCTTAAATAT ATTTGGAGGT GTTGCAGTAA ATCTTTTGGG CTTCATTCAA
    AACTGGAGCA AATCCTCATT TGCTACCCAA GTCAAGTCAA CATACACCAG TCTGGGCAGC
    TCACATCTGG AGCAATCAAC CACACACTCA GAGATCGTGC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173757.1
    CDS314..7600
    Translation

    Target ORF information:

    RefSeq Version XM_005173700.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173700.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TGAGATCAAT
    TTCAAGACTG GGCAGTCTGG CAGTGATGAC ATTGCTTTCC ACTTCAACCC CCAAATGGGC
    CAGAAGGTGG TCATGAACAG CTTCAGGAAT GGAGCATGGG AAACTGAGGA AAGTGTCTCA
    GACAACCCTT TTACAAAGGG ACAAGATTTT GAGATGCTCA CCGTCCTCCA GCCAGAAGGA
    TATCAAGTGT ATGTGAACGG CAAGGTGCTG CACACATTCA AGCACCGTAT GCAACTAGAA
    AAAGTGTCCA CCTTAAATAT ATTTGGAGGT GTTGCAGTAA ATCTTTTGGG CTTCATTCAA
    AACTGGAGCA AATCCTCATT TGCTACCCAA GTCAAGTCAA CATACACCAG TCTGGGCAGC
    TCACATCTGG AGCAATCAAC CACACACTCA GAGATCGTGC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23658
    Clone ID Related Accession (Same CDS sequence) XM_005173701.3 , XM_005173701.4
    Accession Version XM_005173701.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTCAACCCC CAAATGGGCC AGAAGGTGGT CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTGAGGAA AGTGTCTCAG ACAACCCTTT TACAAAGGGA
    CAAGATTTTG AGATGCTCAC CGTCCTCCAG CCAGAAGGAT ATCAAGTGTA TGTGAACGGC
    AAGGTGCTGC ACACATTCAA GCACCGTATG CAACTAGAAA AAGTGTCCAC CTTAAATATA
    TTTGGAGGTG TTGCAGTAAA TCTTTTGGGC TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    GCTACCCAAG TCAAGTCAAC ATACACCAGT CTGGGCAGCT CACATCTGGA GCAATCAACC
    ACACACTCAG AGATCGTGCA AGCAGTCCAA AACCCTAACC TTCCTTATGT GGGCTCAATT
    TCTGGAGGAC TGAGAGAAGG CATGGCCTTG TACTTCCAGG GTGTTGTTCC TCCTAATGCT
    GACAAATTTG AGATCAATTT CAAGACTGGG CAGTCTGGCA GTGATGACAT TGCTTTCCAC
    TTTAACCCCC TAATGGGCCA GAATTTGGAC ATGAACAGCT TCAGGAATGG AGCATGGGAA
    ACTCAGGAGA GTGTCTCCAA CAACCCTTTT AAAAAGGGGG AAGCTTTTGA GATGTTCATG
    GTCATCAAAT CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173758.1
    CDS66..7352
    Translation

    Target ORF information:

    RefSeq Version XM_005173701.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173701.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTCAACCCC CAAATGGGCC AGAAGGTGGT CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTGAGGAA AGTGTCTCAG ACAACCCTTT TACAAAGGGA
    CAAGATTTTG AGATGCTCAC CGTCCTCCAG CCAGAAGGAT ATCAAGTGTA TGTGAACGGC
    AAGGTGCTGC ACACATTCAA GCACCGTATG CAACTAGAAA AAGTGTCCAC CTTAAATATA
    TTTGGAGGTG TTGCAGTAAA TCTTTTGGGC TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    GCTACCCAAG TCAAGTCAAC ATACACCAGT CTGGGCAGCT CACATCTGGA GCAATCAACC
    ACACACTCAG AGATCGTGCA AGCAGTCCAA AACCCTAACC TTCCTTATGT GGGCTCAATT
    TCTGGAGGAC TGAGAGAAGG CATGGCCTTG TACTTCCAGG GTGTTGTTCC TCCTAATGCT
    GACAAATTTG AGATCAATTT CAAGACTGGG CAGTCTGGCA GTGATGACAT TGCTTTCCAC
    TTTAACCCCC TAATGGGCCA GAATTTGGAC ATGAACAGCT TCAGGAATGG AGCATGGGAA
    ACTCAGGAGA GTGTCTCCAA CAACCCTTTT AAAAAGGGGG AAGCTTTTGA GATGTTCATG
    GTCATCAAAT CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23658
    Clone ID Related Accession (Same CDS sequence) XM_005173701.3 , XM_005173701.4
    Accession Version XM_005173701.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173701.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTCAACCCC CAAATGGGCC AGAAGGTGGT CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTGAGGAA AGTGTCTCAG ACAACCCTTT TACAAAGGGA
    CAAGATTTTG AGATGCTCAC CGTCCTCCAG CCAGAAGGAT ATCAAGTGTA TGTGAACGGC
    AAGGTGCTGC ACACATTCAA GCACCGTATG CAACTAGAAA AAGTGTCCAC CTTAAATATA
    TTTGGAGGTG TTGCAGTAAA TCTTTTGGGC TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    GCTACCCAAG TCAAGTCAAC ATACACCAGT CTGGGCAGCT CACATCTGGA GCAATCAACC
    ACACACTCAG AGATCGTGCA AGCAGTCCAA AACCCTAACC TTCCTTATGT GGGCTCAATT
    TCTGGAGGAC TGAGAGAAGG CATGGCCTTG TACTTCCAGG GTGTTGTTCC TCCTAATGCT
    GACAAATTTG AGATCAATTT CAAGACTGGG CAGTCTGGCA GTGATGACAT TGCTTTCCAC
    TTTAACCCCC TAATGGGCCA GAATTTGGAC ATGAACAGCT TCAGGAATGG AGCATGGGAA
    ACTCAGGAGA GTGTCTCCAA CAACCCTTTT AAAAAGGGGG AAGCTTTTGA GATGTTCATG
    GTCATCAAAT CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173758.1
    CDS317..7603
    Translation

    Target ORF information:

    RefSeq Version XM_005173701.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173701.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTCAACCCC CAAATGGGCC AGAAGGTGGT CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTGAGGAA AGTGTCTCAG ACAACCCTTT TACAAAGGGA
    CAAGATTTTG AGATGCTCAC CGTCCTCCAG CCAGAAGGAT ATCAAGTGTA TGTGAACGGC
    AAGGTGCTGC ACACATTCAA GCACCGTATG CAACTAGAAA AAGTGTCCAC CTTAAATATA
    TTTGGAGGTG TTGCAGTAAA TCTTTTGGGC TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    GCTACCCAAG TCAAGTCAAC ATACACCAGT CTGGGCAGCT CACATCTGGA GCAATCAACC
    ACACACTCAG AGATCGTGCA AGCAGTCCAA AACCCTAACC TTCCTTATGT GGGCTCAATT
    TCTGGAGGAC TGAGAGAAGG CATGGCCTTG TACTTCCAGG GTGTTGTTCC TCCTAATGCT
    GACAAATTTG AGATCAATTT CAAGACTGGG CAGTCTGGCA GTGATGACAT TGCTTTCCAC
    TTTAACCCCC TAATGGGCCA GAATTTGGAC ATGAACAGCT TCAGGAATGG AGCATGGGAA
    ACTCAGGAGA GTGTCTCCAA CAACCCTTTT AAAAAGGGGG AAGCTTTTGA GATGTTCATG
    GTCATCAAAT CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23659
    Clone ID Related Accession (Same CDS sequence) XM_005173702.4 , XM_005173702.3
    Accession Version XM_005173702.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173702.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC CCGTTACTCA AGCCAAGTCA
    ACAATCCCCA GCCACGGCAG CACACATGGG AAATGGTCCA GCACACACTT GGAGATCTCG
    CAACCAGTCC AACAACCTAA ACTTGCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGCTGGAT
    AAGAACGTGG CCATGAACAG CGTAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCT
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGTGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAATTAT TCCTTATGTG GGCCAAGTTC CCGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA GATCAACTTC
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TTAAACCCCA AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCAGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATAGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173759.1
    CDS313..7182
    Translation

    Target ORF information:

    RefSeq Version XM_005173702.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173702.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC CCGTTACTCA AGCCAAGTCA
    ACAATCCCCA GCCACGGCAG CACACATGGG AAATGGTCCA GCACACACTT GGAGATCTCG
    CAACCAGTCC AACAACCTAA ACTTGCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGCTGGAT
    AAGAACGTGG CCATGAACAG CGTAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCT
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGTGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAATTAT TCCTTATGTG GGCCAAGTTC CCGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA GATCAACTTC
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TTAAACCCCA AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCAGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATAGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23659
    Clone ID Related Accession (Same CDS sequence) XM_005173702.4 , XM_005173702.3
    Accession Version XM_005173702.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173702.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC CCGTTACTCA AGCCAAGTCA
    ACAATCCCCA GCCACGGCAG CACACATGGG AAATGGTCCA GCACACACTT GGAGATCTCG
    CAACCAGTCC AACAACCTAA ACTTGCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGCTGGAT
    AAGAACGTGG CCATGAACAG CGTAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCT
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGTGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAATTAT TCCTTATGTG GGCCAAGTTC CCGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA GATCAACTTC
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TTAAACCCCA AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCAGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATAGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173759.1
    CDS66..6935
    Translation

    Target ORF information:

    RefSeq Version XM_005173702.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173702.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC CCGTTACTCA AGCCAAGTCA
    ACAATCCCCA GCCACGGCAG CACACATGGG AAATGGTCCA GCACACACTT GGAGATCTCG
    CAACCAGTCC AACAACCTAA ACTTGCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGCTGGAT
    AAGAACGTGG CCATGAACAG CGTAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCT
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGTGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAATTAT TCCTTATGTG GGCCAAGTTC CCGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA GATCAACTTC
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TTAAACCCCA AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCAGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATAGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23660
    Clone ID Related Accession (Same CDS sequence) XM_005173703.3 , XM_005173703.4
    Accession Version XM_005173703.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173760.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173703.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173703.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23660
    Clone ID Related Accession (Same CDS sequence) XM_005173703.3 , XM_005173703.4
    Accession Version XM_005173703.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173703.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173760.1
    CDS319..7188
    Translation

    Target ORF information:

    RefSeq Version XM_005173703.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173703.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23661
    Clone ID Related Accession (Same CDS sequence) XM_005173704.3 , XM_005173704.4
    Accession Version XM_005173704.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173761.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173704.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173704.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23661
    Clone ID Related Accession (Same CDS sequence) XM_005173704.3 , XM_005173704.4
    Accession Version XM_005173704.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173704.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173761.1
    CDS318..7187
    Translation

    Target ORF information:

    RefSeq Version XM_005173704.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173704.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23662
    Clone ID Related Accession (Same CDS sequence) XM_005173705.4 , XM_005173705.3
    Accession Version XM_005173705.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173705.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173762.1
    CDS319..7188
    Translation

    Target ORF information:

    RefSeq Version XM_005173705.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173705.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23662
    Clone ID Related Accession (Same CDS sequence) XM_005173705.4 , XM_005173705.3
    Accession Version XM_005173705.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173762.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173705.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173705.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23663
    Clone ID Related Accession (Same CDS sequence) XM_005173706.3 , XM_005173706.4
    Accession Version XM_005173706.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173763.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173706.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173706.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23663
    Clone ID Related Accession (Same CDS sequence) XM_005173706.3 , XM_005173706.4
    Accession Version XM_005173706.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173706.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173763.1
    CDS318..7187
    Translation

    Target ORF information:

    RefSeq Version XM_005173706.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173706.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23664
    Clone ID Related Accession (Same CDS sequence) XM_005173707.3 , XM_005173707.4
    Accession Version XM_005173707.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173764.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173707.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173707.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23664
    Clone ID Related Accession (Same CDS sequence) XM_005173707.3 , XM_005173707.4
    Accession Version XM_005173707.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173707.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173764.1
    CDS319..7188
    Translation

    Target ORF information:

    RefSeq Version XM_005173707.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173707.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23665
    Clone ID Related Accession (Same CDS sequence) XM_005173708.4 , XM_005173708.3
    Accession Version XM_005173708.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173708.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA GATCAACTTC
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TTAAACCCCA AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCAGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATAGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173765.1
    CDS562..7431
    Translation

    Target ORF information:

    RefSeq Version XM_005173708.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173708.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA GATCAACTTC
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TTAAACCCCA AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCAGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATAGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23665
    Clone ID Related Accession (Same CDS sequence) XM_005173708.4 , XM_005173708.3
    Accession Version XM_005173708.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X17
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA GATCAACTTC
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TTAAACCCCA AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCAGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATAGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173765.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173708.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173708.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA GATCAACTTC
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TTAAACCCCA AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCAGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATAGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23666
    Clone ID Related Accession (Same CDS sequence) XM_005173709.3 , XM_005173709.4
    Accession Version XM_005173709.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173709.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173766.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173709.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173709.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23666
    Clone ID Related Accession (Same CDS sequence) XM_005173709.3 , XM_005173709.4
    Accession Version XM_005173709.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173709.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173766.1
    CDS318..7187
    Translation

    Target ORF information:

    RefSeq Version XM_005173709.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173709.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23667
    Clone ID Related Accession (Same CDS sequence) XM_005173710.4 , XM_005173710.3
    Accession Version XM_005173710.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173710.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173767.1
    CDS561..7430
    Translation

    Target ORF information:

    RefSeq Version XM_005173710.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173710.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23667
    Clone ID Related Accession (Same CDS sequence) XM_005173710.4 , XM_005173710.3
    Accession Version XM_005173710.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173767.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173710.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173710.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23668
    Clone ID Related Accession (Same CDS sequence) XM_005173711.3 , XM_005173711.4
    Accession Version XM_005173711.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173768.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_005173711.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173711.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23668
    Clone ID Related Accession (Same CDS sequence) XM_005173711.3 , XM_005173711.4
    Accession Version XM_005173711.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173711.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173768.1
    CDS562..7431
    Translation

    Target ORF information:

    RefSeq Version XM_005173711.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173711.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23669
    Clone ID Related Accession (Same CDS sequence) XM_005173712.4 , XM_005173712.3
    Accession Version XM_005173712.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173712.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTAT ATGTAAACGA AAACGACCTG TACACATTCA AGCACCGTAT ACCACTGGAA
    AAAGTGTCCA TCTTGAATAT TGTTGGAGCG GTTTCTATGA ACATTTTTGG GTTCATTCAA
    AACTGGAGCA AATCCTCATT TACTACTAGA GTCAAGTCAA CAATCACCAG TCTGGGAAGC
    CCACAGTGGG ATCTGTCCAT AATACAATCA AAGGCCTTAC AAGCAGTGCA AAATCCCAAC
    CTTCCTTATT TGGGCCAAAT CCCTGGAGGA CTAAGAGAAG GCATGACCTT GTATGTGAAG
    GGAGTTGTTC CTAGTAATGG TGATCGGTTT TCAATCAACT TCAAGACCGG GTCTACTGAT
    AAGGATGACA TTGCTTTCCA CTTCAATCCC CGAATGGGCT CAAAGCTGGT CATGAACAGT
    ATGAAGAGCG GGAGATGGGG AGCGGAGGAA TACGTCTCTG AAAACCCGTG TAAAAAGGGA
    GATGCTTTTG AGTTCTACAT TCAGATCAAA GCTGAAGGAT ATCAGGTGCA ATTCAAAGAC
    CACAAGCAGA GCATGTTCAA ACACCGCATC CCTTTTGAAA GAGTGACTAC AATCAACGTT
    CTGGGGAATG TCTCTATCAG CTTTCTTGGA TTTGTCATGG GGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173769.1
    CDS314..7081
    Translation

    Target ORF information:

    RefSeq Version XM_005173712.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173712.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTAT ATGTAAACGA AAACGACCTG TACACATTCA AGCACCGTAT ACCACTGGAA
    AAAGTGTCCA TCTTGAATAT TGTTGGAGCG GTTTCTATGA ACATTTTTGG GTTCATTCAA
    AACTGGAGCA AATCCTCATT TACTACTAGA GTCAAGTCAA CAATCACCAG TCTGGGAAGC
    CCACAGTGGG ATCTGTCCAT AATACAATCA AAGGCCTTAC AAGCAGTGCA AAATCCCAAC
    CTTCCTTATT TGGGCCAAAT CCCTGGAGGA CTAAGAGAAG GCATGACCTT GTATGTGAAG
    GGAGTTGTTC CTAGTAATGG TGATCGGTTT TCAATCAACT TCAAGACCGG GTCTACTGAT
    AAGGATGACA TTGCTTTCCA CTTCAATCCC CGAATGGGCT CAAAGCTGGT CATGAACAGT
    ATGAAGAGCG GGAGATGGGG AGCGGAGGAA TACGTCTCTG AAAACCCGTG TAAAAAGGGA
    GATGCTTTTG AGTTCTACAT TCAGATCAAA GCTGAAGGAT ATCAGGTGCA ATTCAAAGAC
    CACAAGCAGA GCATGTTCAA ACACCGCATC CCTTTTGAAA GAGTGACTAC AATCAACGTT
    CTGGGGAATG TCTCTATCAG CTTTCTTGGA TTTGTCATGG GGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23669
    Clone ID Related Accession (Same CDS sequence) XM_005173712.4 , XM_005173712.3
    Accession Version XM_005173712.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X19
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTAT ATGTAAACGA AAACGACCTG TACACATTCA AGCACCGTAT ACCACTGGAA
    AAAGTGTCCA TCTTGAATAT TGTTGGAGCG GTTTCTATGA ACATTTTTGG GTTCATTCAA
    AACTGGAGCA AATCCTCATT TACTACTAGA GTCAAGTCAA CAATCACCAG TCTGGGAAGC
    CCACAGTGGG ATCTGTCCAT AATACAATCA AAGGCCTTAC AAGCAGTGCA AAATCCCAAC
    CTTCCTTATT TGGGCCAAAT CCCTGGAGGA CTAAGAGAAG GCATGACCTT GTATGTGAAG
    GGAGTTGTTC CTAGTAATGG TGATCGGTTT TCAATCAACT TCAAGACCGG GTCTACTGAT
    AAGGATGACA TTGCTTTCCA CTTCAATCCC CGAATGGGCT CAAAGCTGGT CATGAACAGT
    ATGAAGAGCG GGAGATGGGG AGCGGAGGAA TACGTCTCTG AAAACCCGTG TAAAAAGGGA
    GATGCTTTTG AGTTCTACAT TCAGATCAAA GCTGAAGGAT ATCAGGTGCA ATTCAAAGAC
    CACAAGCAGA GCATGTTCAA ACACCGCATC CCTTTTGAAA GAGTGACTAC AATCAACGTT
    CTGGGGAATG TCTCTATCAG CTTTCTTGGA TTTGTCATGG GGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173769.1
    CDS66..6833
    Translation

    Target ORF information:

    RefSeq Version XM_005173712.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173712.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTAT ATGTAAACGA AAACGACCTG TACACATTCA AGCACCGTAT ACCACTGGAA
    AAAGTGTCCA TCTTGAATAT TGTTGGAGCG GTTTCTATGA ACATTTTTGG GTTCATTCAA
    AACTGGAGCA AATCCTCATT TACTACTAGA GTCAAGTCAA CAATCACCAG TCTGGGAAGC
    CCACAGTGGG ATCTGTCCAT AATACAATCA AAGGCCTTAC AAGCAGTGCA AAATCCCAAC
    CTTCCTTATT TGGGCCAAAT CCCTGGAGGA CTAAGAGAAG GCATGACCTT GTATGTGAAG
    GGAGTTGTTC CTAGTAATGG TGATCGGTTT TCAATCAACT TCAAGACCGG GTCTACTGAT
    AAGGATGACA TTGCTTTCCA CTTCAATCCC CGAATGGGCT CAAAGCTGGT CATGAACAGT
    ATGAAGAGCG GGAGATGGGG AGCGGAGGAA TACGTCTCTG AAAACCCGTG TAAAAAGGGA
    GATGCTTTTG AGTTCTACAT TCAGATCAAA GCTGAAGGAT ATCAGGTGCA ATTCAAAGAC
    CACAAGCAGA GCATGTTCAA ACACCGCATC CCTTTTGAAA GAGTGACTAC AATCAACGTT
    CTGGGGAATG TCTCTATCAG CTTTCTTGGA TTTGTCATGG GGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45083
    Clone ID Related Accession (Same CDS sequence) XM_009293898.3 , XM_009293898.2
    Accession Version XM_009293898.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293898.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCCTCATT TGCTACCCAA GTCAAGTCAA CATACACCAG TCTGGGCAGC
    TCACATCTGG AGCAATCAAC CACACACTCA GAGATCGTGC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292173.1
    CDS315..7601
    Translation

    Target ORF information:

    RefSeq Version XM_009293898.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293898.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCCTCATT TGCTACCCAA GTCAAGTCAA CATACACCAG TCTGGGCAGC
    TCACATCTGG AGCAATCAAC CACACACTCA GAGATCGTGC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45083
    Clone ID Related Accession (Same CDS sequence) XM_009293898.3 , XM_009293898.2
    Accession Version XM_009293898.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCCTCATT TGCTACCCAA GTCAAGTCAA CATACACCAG TCTGGGCAGC
    TCACATCTGG AGCAATCAAC CACACACTCA GAGATCGTGC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292173.1
    CDS66..7352
    Translation

    Target ORF information:

    RefSeq Version XM_009293898.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293898.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCCTCATT TGCTACCCAA GTCAAGTCAA CATACACCAG TCTGGGCAGC
    TCACATCTGG AGCAATCAAC CACACACTCA GAGATCGTGC AAGCAGTCCA AAACCCTAAC
    CTTCCTTATG TGGGCTCAAT TTCTGGAGGA CTGAGAGAAG GCATGGCCTT GTACTTCCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56012
    Clone ID Related Accession (Same CDS sequence) XM_017352155.1 , XM_017352155.2
    Accession Version XM_017352155.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207644.1
    CDS66..7352
    Translation

    Target ORF information:

    RefSeq Version XM_017352155.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56012
    Clone ID Related Accession (Same CDS sequence) XM_017352155.1 , XM_017352155.2
    Accession Version XM_017352155.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207644.1
    CDS315..7601
    Translation

    Target ORF information:

    RefSeq Version XM_017352155.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352155.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TCAAATCAAT
    TTTAAGACTG GGCAGTCTGG CAGTGATGAC ACTGCTTTCC ACTTTAACCC CCGAATGGGC
    CAGATTGTGG CCATGAACAG CTTCAGGAAC GGAAAATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCAT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA CTGTCATCAA ATCTGAAGGA
    TATCAGGTGT ATGTGAATGG CAAGGAACAT TACACATTCA AGCACCGAAT ACCACTGGAA
    AAAGTGTCCA CATTGAGTAT TGTTGGAGGA GTTTCAGTCA AGCTTTTGGG CTTTATTGAA
    AACTGGAGCA AATCTTCATT TTGTACCAAA GTCAAGTCCA CTATCACCTG TATGGGAAGC
    TCACACAAGG AACAGTCCAG CATACACTCG GAGATCTCTC AAGCAGTCCA AAACCCTAAT
    CTTCCTTATG TTGGCCCCAT TTCAGGAGGG CTGAGAGAAG GCATGGCCGT GTACTTGCAG
    GGTGTTGTTC CTCCTAATGC TGACAAATTT GAGATCAATT TCAAGACTGG GCAGTCTGGC
    AGTGATGACA TTGCTTTCCA CTTTAACCCC CTAATGGGCC AGAATTTGGA CATGAACAGC
    TTCAGGAATG GAGCATGGGA AACTCAGGAG AGTGTCTCCA ACAACCCTTT TAAAAAGGGG
    GAAGCTTTTG AGATGTTCAT GGTCATCAAA TCTGAAGGAT ATCAGGTATA TGTAAACGAA
    AACGACCTGT ACACATTCAA GCACCGTATA CCACTGGAAA AAGTGTCCAT CTTGAATATT
    GTTGGAGCGG TTTCTATGAA CATTTTTGGG TTCATTCAAA ACTGGAGCAA ATCCTCATTT
    ACTACTAGAG TCAAGTCAAC AATCACCAGT CTGGGAAGCC CACAGTGGGA TCTGTCCATA
    ATACAATCAA AGGCCTTACA AGCAGTGCAA AATCCCAACC TTCCTTATTT GGGCCAAATC
    CCTGGAGGAC TAAGAGAAGG CATGACCTTG TATGTGAAGG GAGTTGTTCC TAGTAATGGT
    GATCGGTTTT CAATCAACTT CAAGACCGGG TCTACTGATA AGGATGACAT TGCTTTCCAC
    TTCAATCCCC GAATGGGCTC AAAGCTGGTC ATGAACAGTA TGAAGAGCGG GAGATGGGGA
    GCGGAGGAAT ACGTCTCTGA AAACCCGTGT AAAAAGGGAG ATGCTTTTGA GTTCTACATT
    CAGATCAAAG CTGAAGGATA TCAGGTGCAA TTCAAAGACC ACAAGCAGAG CATGTTCAAA
    CACCGCATCC CTTTTGAAAG AGTGACTACA ATCAACGTTC TGGGGAATGT CTCTATCAGC
    TTTCTTGGAT TTGTCATGGG GATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56013
    Clone ID Related Accession (Same CDS sequence) XM_017352156.1 , XM_017352156.2
    Accession Version XM_017352156.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X16
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207645.1
    CDS67..6936
    Translation

    Target ORF information:

    RefSeq Version XM_017352156.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56013
    Clone ID Related Accession (Same CDS sequence) XM_017352156.1 , XM_017352156.2
    Accession Version XM_017352156.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207645.1
    CDS562..7431
    Translation

    Target ORF information:

    RefSeq Version XM_017352156.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352156.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAATCTT
    CCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCGGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AAATATTCCT
    TATGTGGGCC AGGTTCCTGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AATTTTAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTCAA CCCCCGAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CTTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCT GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCTCAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAGCTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CATCAATCCC CAGCCTGGGA AGGTCATCTG GAAAATGGTC CACCACACAC
    TTGGAGATCT CACAACCAGT CCAACAACCT AAACTTCATA AGGTGGTCCA GATTCCTGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTTGC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAAGT TCCCGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TCAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTTAAACCC
    CAAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCCTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCAGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCACATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAA CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCT GTTGCTCAAG CCAGGTCAGC AATACCCAGC
    CAGGGCAGCT CTCATGGGAA ATGGTCCAGC ACACACTTGG AGATCTCGCA ACCAGTCCAA
    CACCCTAAAC TTACTAAGGC GGTCCAGATT CCCGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTGACT ATGATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCA GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTCACAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGA CCAAATTCCT GGAGGACTGA GAGAAGGCAT GGCCATGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTC AACCCCCGAA TGGGCCAGAA GGTGACCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTAAA
    AAAGGACAAC ATTTTAGTAT GTTGACTGCC ATCACATCTG CTGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACTTGTTC CTCAAGCCAA GTCAACAATC TCCAGCCTGG GCGGCTCACA TGGGGAACAG
    TCTACCACAC ACTTGGAGAT CTCACAACCA GTCCAACACC CTAGCATTCC TTATGTTGGC
    CCCATTTCAG GAGGACTGAG AGAGGGCATG GCCGTGTACT TGCAGGGTGT TGTTCCTCCT
    AATGCTGACA AATTTCAAAT CAATTTTAAG ACTGGGCAGT CTGGCAGTGA TGACACTGCT
    TTCCACTTTA ACCCCCGAAT GGGCCAGATT GTGGCCATGA ACAGCTTCAG GAACGGAAAA
    TGGGAAACTC AGGAGAGTGT CTCCAACAAC CCATTTAAAA AGGGGGAAGC TTTTGAGATG
    TTCACTGTCA TCAAATCTGA AGGATATCAG GTGTATGTGA ATGGCAAGGA ACATTACACA
    TTCAAGCACC GAATACCACT GGAAAAAGTG TCCACATTGA GTATTGTTGG AGGAGTTTCA
    GTCAAGCTTT TGGGCTTTAT TGAAAACTGG AGCAAATCTT CATTTTGTAC CAAAGTCAAG
    TCCACTATCA CCTGTATGGG AAGCTCACAC AAGGAACAGT CCAGCATACA CTCGGAGATC
    TCTCAAGCAG TCCAAAACCC TAATCTTCCT TATGTTGGCC CCATTTCAGG AGGGCTGAGA
    GAAGGCATGG CCGTGTACTT GCAGGGTGTT GTTCCTCCTA ATGCTGACAA ATTTGAGATC
    AATTTCAAGA CTGGGCAGTC TGGCAGTGAT GACATTGCTT TCCACTTCAA CCCCCAAATG
    GGCCAGAAGG TGGTCATGAA CAGCTTCAGG AATGGAGCAT GGGAAACTGA GGAAAGTGTC
    TCAGACAACC CTTTTACAAA GGGACAAGAT TTTGAGATGC TCACCGTCCT CCAGCCAGAA
    GGATATCAAG TGTATGTGAA CGGCAAGGTG CTGCACACAT TCAAGCACCG TATGCAACTA
    GAAAAAGTGT CCACCTTAAA TATATTTGGA GGTGTTGCAG TAAATCTTTT GGGCTTCATT
    CAAAACTGGA GCAAATCCTC ATTTGCTACC CAAGTCAAGT CAACATACAC CAGTCTGGGC
    AGCTCACATC TGGAGCAATC AACCACACAC TCAGAGATCG TGCAAGCAGT CCAAAACCCT
    AACCTTCCTT ATGTGGGCTC AATTTCTGGA GGACTGAGAG AAGGCATGGC CTTGTACTTC
    CAGGGTGTTG TTCCTCCTAA TGCTGACAAA TTTGAGATCA ATTTCAAGAC TGGGCAGTCT
    GGCAGTGATG ACATTGCTTT CCACTTTAAC CCCCTAATGG GCCAGAATTT GGACATGAAC
    AGCTTCAGGA ATGGAGCATG GGAAACTCAG GAGAGTGTCT CCAACAACCC TTTTAAAAAG
    GGGGAAGCTT TTGAGATGTT CATGGTCATC AAATCTGAAG GATATCAGGT ATATGTAAAC
    GAAAACGACC TGTACACATT CAAGCACCGT ATACCACTGG AAAAAGTGTC CATCTTGAAT
    ATTGTTGGAG CGGTTTCTAT GAACATTTTT GGGTTCATTC AAAACTGGAG CAAATCCTCA
    TTTACTACTA GAGTCAAGTC AACAATCACC AGTCTGGGAA GCCCACAGTG GGATCTGTCC
    ATAATACAAT CAAAGGCCTT ACAAGCAGTG CAAAATCCCA ACCTTCCTTA TTTGGGCCAA
    ATCCCTGGAG GACTAAGAGA AGGCATGACC TTGTATGTGA AGGGAGTTGT TCCTAGTAAT
    GGTGATCGGT TTTCAATCAA CTTCAAGACC GGGTCTACTG ATAAGGATGA CATTGCTTTC
    CACTTCAATC CCCGAATGGG CTCAAAGCTG GTCATGAACA GTATGAAGAG CGGGAGATGG
    GGAGCGGAGG AATACGTCTC TGAAAACCCG TGTAAAAAGG GAGATGCTTT TGAGTTCTAC
    ATTCAGATCA AAGCTGAAGG ATATCAGGTG CAATTCAAAG ACCACAAGCA GAGCATGTTC
    AAACACCGCA TCCCTTTTGA AAGAGTGACT ACAATCAACG TTCTGGGGAA TGTCTCTATC
    AGCTTTCTTG GATTTGTCAT GGGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56014
    Clone ID Related Accession (Same CDS sequence) XM_017352157.1 , XM_017352157.2
    Accession Version XM_017352157.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X18
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TGAGATCAAT
    TTCAAGACTG GGCAGTCTGG CAGTGATGAC ATTGCTTTCC ACTTTAACCC CCTAATGGGC
    CAGAATTTGG ACATGAACAG CTTCAGGAAT GGAGCATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCTT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA TGGTCATCAA ATCTGAAGGA
    TATCAGGTAT ATGTAAACGA AAACGACCTG TACACATTCA AGCACCGTAT ACCACTGGAA
    AAAGTGTCCA TCTTGAATAT TGTTGGAGCG GTTTCTATGA ACATTTTTGG GTTCATTCAA
    AACTGGAGCA AATCCTCATT TACTACTAGA GTCAAGTCAA CAATCACCAG TCTGGGAAGC
    CCACAGTGGG ATCTGTCCAT AATACAATCA AAGGCCTTAC AAGCAGTGCA AAATCCCAAC
    CTTCCTTATT TGGGCCAAAT CCCTGGAGGA CTAAGAGAAG GCATGACCTT GTATGTGAAG
    GGAGTTGTTC CTAGTAATGG TGATCGGTTT TCAATCAACT TCAAGACCGG GTCTACTGAT
    AAGGATGACA TTGCTTTCCA CTTCAATCCC CGAATGGGCT CAAAGCTGGT CATGAACAGT
    ATGAAGAGCG GGAGATGGGG AGCGGAGGAA TACGTCTCTG AAAACCCGTG TAAAAAGGGA
    GATGCTTTTG AGTTCTACAT TCAGATCAAA GCTGAAGGAT ATCAGGTGCA ATTCAAAGAC
    CACAAGCAGA GCATGTTCAA ACACCGCATC CCTTTTGAAA GAGTGACTAC AATCAACGTT
    CTGGGGAATG TCTCTATCAG CTTTCTTGGA TTTGTCATGG GGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207646.1
    CDS66..6833
    Translation

    Target ORF information:

    RefSeq Version XM_017352157.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TGAGATCAAT
    TTCAAGACTG GGCAGTCTGG CAGTGATGAC ATTGCTTTCC ACTTTAACCC CCTAATGGGC
    CAGAATTTGG ACATGAACAG CTTCAGGAAT GGAGCATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCTT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA TGGTCATCAA ATCTGAAGGA
    TATCAGGTAT ATGTAAACGA AAACGACCTG TACACATTCA AGCACCGTAT ACCACTGGAA
    AAAGTGTCCA TCTTGAATAT TGTTGGAGCG GTTTCTATGA ACATTTTTGG GTTCATTCAA
    AACTGGAGCA AATCCTCATT TACTACTAGA GTCAAGTCAA CAATCACCAG TCTGGGAAGC
    CCACAGTGGG ATCTGTCCAT AATACAATCA AAGGCCTTAC AAGCAGTGCA AAATCCCAAC
    CTTCCTTATT TGGGCCAAAT CCCTGGAGGA CTAAGAGAAG GCATGACCTT GTATGTGAAG
    GGAGTTGTTC CTAGTAATGG TGATCGGTTT TCAATCAACT TCAAGACCGG GTCTACTGAT
    AAGGATGACA TTGCTTTCCA CTTCAATCCC CGAATGGGCT CAAAGCTGGT CATGAACAGT
    ATGAAGAGCG GGAGATGGGG AGCGGAGGAA TACGTCTCTG AAAACCCGTG TAAAAAGGGA
    GATGCTTTTG AGTTCTACAT TCAGATCAAA GCTGAAGGAT ATCAGGTGCA ATTCAAAGAC
    CACAAGCAGA GCATGTTCAA ACACCGCATC CCTTTTGAAA GAGTGACTAC AATCAACGTT
    CTGGGGAATG TCTCTATCAG CTTTCTTGGA TTTGTCATGG GGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56014
    Clone ID Related Accession (Same CDS sequence) XM_017352157.1 , XM_017352157.2
    Accession Version XM_017352157.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein si:dkey-151j17.4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TGAGATCAAT
    TTCAAGACTG GGCAGTCTGG CAGTGATGAC ATTGCTTTCC ACTTTAACCC CCTAATGGGC
    CAGAATTTGG ACATGAACAG CTTCAGGAAT GGAGCATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCTT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA TGGTCATCAA ATCTGAAGGA
    TATCAGGTAT ATGTAAACGA AAACGACCTG TACACATTCA AGCACCGTAT ACCACTGGAA
    AAAGTGTCCA TCTTGAATAT TGTTGGAGCG GTTTCTATGA ACATTTTTGG GTTCATTCAA
    AACTGGAGCA AATCCTCATT TACTACTAGA GTCAAGTCAA CAATCACCAG TCTGGGAAGC
    CCACAGTGGG ATCTGTCCAT AATACAATCA AAGGCCTTAC AAGCAGTGCA AAATCCCAAC
    CTTCCTTATT TGGGCCAAAT CCCTGGAGGA CTAAGAGAAG GCATGACCTT GTATGTGAAG
    GGAGTTGTTC CTAGTAATGG TGATCGGTTT TCAATCAACT TCAAGACCGG GTCTACTGAT
    AAGGATGACA TTGCTTTCCA CTTCAATCCC CGAATGGGCT CAAAGCTGGT CATGAACAGT
    ATGAAGAGCG GGAGATGGGG AGCGGAGGAA TACGTCTCTG AAAACCCGTG TAAAAAGGGA
    GATGCTTTTG AGTTCTACAT TCAGATCAAA GCTGAAGGAT ATCAGGTGCA ATTCAAAGAC
    CACAAGCAGA GCATGTTCAA ACACCGCATC CCTTTTGAAA GAGTGACTAC AATCAACGTT
    CTGGGGAATG TCTCTATCAG CTTTCTTGGA TTTGTCATGG GGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207646.1
    CDS314..7081
    Translation

    Target ORF information:

    RefSeq Version XM_017352157.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151j17.4 (si:dkey-151j17.4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352157.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGCTTCA AATTAAAGTC GGGCTGCAGG TCAAGCTGCC ACACCACCTC CGGCTTTAGC 
    GTCCCTTATG TGAGCCGAAT TCATGGAGGA CTGAAAGCTG GGAAAGGCGT CTTCATCCAG
    GGAGCTGTTC CTGCTGGATC TGATGGCTTT GTGGTGAATC TGAAGTGTGG AGAGTCTGAC
    GGGGATGACA TCGCCTTTCA AATGAAGCCT CAGTTTATCA GTAACTTCAC TGCGGTAAAC
    AGCCGCCAAA ACGGATCATG GGGAAAAGAG GAGAAACTGG ACTTTCCTTT AAAACCGGGG
    AGCAGTTTTG ATCTGAACAT TGCAGTGAAT TCTGAAGGTT ATCAGGTGTT TTTGAGTGGA
    CAAGAAGTCG GTTGTTTTCA GCATCGTATA TCTCTGGAAC GCGTCAACAC TCTGGCTGTC
    GCAGGAGGGG TTTCTTTAAC AAACGTGATG TTCACCGAGG GCTGGTCTGG GTCTTCTAGC
    ATCAGGGGTG GCAGAGGCAT GCATCACTCA CATCATGGGG TTTTTAGAGG ACATTCATCT
    ATGCGGGAGG GAACCTGGTC TTTCACTGAG ACCAAACATC ATCCGTCAAC TACCCATCTG
    ACAACTGAAC TTTCCTTAAG CAACCCAGTC CAAAATCCTA AACTCCCTTA TGTGGGCCCT
    ATTTCAGGAG GACTGAGAGA AGGCATGGCC TTGTATGTCA AAGGAGTTGT TCCTAATAAT
    GCTGATCGAT TTGCACTAAA TTTCAAGACT GGGATTTCTG ACAAGGATGA CGTTGCTTTC
    CACTTCAACC CTCGAATGGA GCAGAAGGTG ACCATGAACA GTTTCCAGAA CGGAGCATGG
    GGAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAGG GGCAAGATTT TGAGATGTTG
    ACTGCCATCA CATCTGCAGG ATATCAGGTT TATGTGAACG GCAAGGAACT GTACACATTC
    AAGCATCATA TACCACTGGA AAAGGTGGCA GTGTTGAATA TTGGTGGAGA TGTAACCCTG
    AACCTTTTTG GCTTCATTCA AAACTTGAGC ACTTCTGCAT TCCCTACCAG AGACACAAAA
    ACATTCACCA GCCTCGGCAG CTCACATGGG AAATGGTCCA GCACACACTA TGAAATTTCA
    CAACCAGTCC AACACCCTAA TCTTCCTCGT GTGGTCCAGA TTCCCGGAGG ACTGAAAGAA
    GGCATGATCT TGTACATGAA GGGAGCTGTT CCTGCCAATG CTGACAGATT TGAAATCAAT
    TTCAAGACTG GGCAGTCTGG TGATGATGAC ACTGCTTTCC ATTTCAACCC TCGGATGGAT
    AAGAACGTGG CCATGAACAG CATAATAAAT GGACAATCTG GAACAGAAGA AATTATCTCC
    GACAACCCTT TTAAAAAGGG AGAACCATTT GAGATGTTAA CTATCATCAA ACCTGAAGGA
    TATCAGGTGC AAGTGAATGG CAAAGAACAG TGCATATTCA AGCACCGTAC ACCTCTTGAA
    AGAGTGTCAG CATTAAATAT CAGCGGAGAC GTTGCTGTAA ACAACTTGGA GATCTCACAG
    TCAATCCAAA ACCCAAATAT TCCTTATGTG GGCCAAGTTC CTGGAGGACT GAGAGAAGGC
    ATGGCCCTGT TCATGCAGGG TGTTGTTCCT GATAATGCTG ACCAATTTGA AATCAATTTT
    AAGACTGGGC AGTCTGGCAG TGATGACATT GCTTTCCACT TCAACCCCCG AATGGGCCAG
    AAGGTGGTCA TGAACAGCTT CAGGAATGGA GCATGGGAAA CTGAGGAAAG TGTCTCAGAC
    AACCCCTTTA CAAAGGGACA ACACTTTAAG ATGCTGACTG CCATCACATC TGCTGGATAT
    CAGGTGTACG TGAATGACAA AGAACTGTGC ACATTCAAGC ACCGTCTACC ACTGGAAAGA
    TTGTCAACAT TAAATATCAG TGGAAATGTT GCCATCAACT CTCTTGGCTC CATACAAAAC
    TGGAGCACAT CCTCACCCGT TACTCAAGCC AAGTCAACAA TCCCCAGCCA CGGCAGCACA
    CATGGGAAAT GGTCCAGCAC ACACTTGGAG ATCTCGCAAC CAGTCCAACA ACCTAAACTT
    GCTCGTGTGG TCCAGATTCC CGGAGGACTG AAAGAAGGCA TGATCTTGTA CATGAAGGGA
    GCTGTTCCTG CCAATGCTGA CAGATTTGAA ATCAATTTCA AGACTGGGCA GTCTGGTGAT
    GATGACACTG CTTTCCATTT CAACCCTCGG CTGGATAAGA ACGTGGCCAT GAACAGCGTA
    ATAAATGGAC AATCTGGAAC AGAAGAAATT ATCTCTGACA ACCCTTTTAA AAAGGGAGAA
    CCATTTGAGA TGTTAACTAT CATCAAACCT GAAGGATATC AGGTGCAAGT GAATGGCAAA
    GAACAGTGCA TATTCAAGCA CCGTACACCT CTTGAAAGAG TGTCAGCATT AAATATCAGT
    GGAGACGTTG CTGTAAACAA CTTGGAGATC TCACAGTCAA TCCAAAACCC AATTATTCCT
    TATGTGGGCC AAGTTCCCGG AGGACTGAGA GAAGGCATGG CCCTGTTCAT GCAGGGTGTT
    GTTCCTGATA ATGCTGACCA ATTTGAGATC AACTTCAAGA CTGGGCAGTC TGGCAGTGAT
    GACATTGCTT TCCACTTTAA ACCCCAAATG GGCCAGAAGG TGGTCATGAA CAGCTTCAGG
    AATGGAGCAT GGGAAACTGA GGAAAGTGTC TCAGACAACC CCTTTACAAA GGGACAACAC
    TTTAAGATGC TGACTGCCAT CACATCTGCA GGATATCAGG TGTACGTGAA TGACAAAGAA
    CTGTGCACAT TCAAGCACCG TCTACCACTG GAAAGATTGT CAACATTAAA TATCAGTGGA
    AATGTTGCCA TCAACTCTCT TGGCTCCATA CAAAACTGGA GCACATCCTC ACCCGTTACT
    CAAGCCAAGT CAACAATCCC CAGCCACGGC AGCACACATA GGAAATGGTC CAGCACACAC
    TTGGAGATCT CGCAACCAGT CCAACAACCT AATCTTCCTC GTGTGGTCCA GATTCCCGGA
    GGACTGAAAG AAGGCATGAT CTTGTACATG AAGGGAGCTG TTCCTGCCAA TGCTGACAGA
    TTTGAAATCA ATTTCAAGAC TGGGCAGTCT GGTGATGATG ACACTGCTTT CCATTTCAAC
    CCTCGGCTGG ATAAGAACGT GGCCATGAAC AGCGTAATAA ATGGACAATC TGGAACAGAA
    GAAATTATCT CTGACAACCC TTTTAAAAAG GGAGAACCAT TTGAGATGTT AACTATCATC
    AAACCTGAAG GATATCAGGT GCAAGTGAAT GGCAAAGAAC AGTGCATATT CAAGCACCGT
    ACACCTCTTG AAAGAGTGTC AGCATTAAAT ATCGGTGGAG ACGTTGCTGT AAACAACTTG
    GAGATCTCAC AGTCAATCCA AAACCCAAAT ATTCCTTATG TGGGCCAGGT TCCTGGAGGA
    CTGAGAGAAG GCATGGCCCT GTTCATGCAG GGTGTTGTTC CTGATAATGC TGACCAATTT
    GAGATCAATT TTAAGACTGG GCAGTCTGGC AGTGATGACA TTGCTTTCCA CTTCAACCCC
    CGAATGGGCC AGAAGGTGGT CATGAACAGC TTCAGGAATG GAGCATGGGA AACTGAGGAA
    AGTGTCTCAG ACAACCCTTT TACAAAGGGA CAACACTTTA AGATGCTGAC TGCCATCACA
    TCTGCTGGAT ATCAGGTGTA CGTGAATGAC AAAGAACTGT GCTCATTCAA GCACCGTCTA
    CCACTGGAAA GATTGTCAAC ATTAAATATC AGTGGAAATG TTGCCATCAG CTCTCTTGGC
    TCCATACAAA ACTGGAGCAC ATCCTCACCC GTTACTCAAG CCAAGTCATC AATCCCCAGC
    CTGGGAAGGT CATCTGGAAA ATGGTCCACC ACACACTTGG AGATCTCACA ACCAGTCCAA
    CAACCTAAAC TTCATAAGGT GGTCCAGATT CCTGGAGGAC TGAAAGAAGG CATGATCTTG
    TACATGAAGG GAGCTGTTCC TGCCAATGCT GACAGATTTG AAATCAATTT CAAGACTGGG
    CAGTCTGGTG ATGATGACAC TGCTTTCCAT TTCAACCCTC GGCTGGATAA GAACGTGGCC
    ATGAACAGCG TAATAAATGG ACAATCTGGA ACAGAAGAAA TTATCTCTGA CAACCCTTTT
    AAAAAGGGAG AACCATTTGA GATGTTAACT ATCATCAAAC CTGAAGGATA TCAGGTGCAA
    GTGAATGGCA AAGAACAGTG CATATTCAAG CACCGTACAC CTCTTGAAAG AGTGTCAGCA
    TTAAATATCG GTGGAGACGT TGCTGTAAAC AACTTGGAGA TCTTGCAGTC AATCCAAAAC
    CCAAATATTC CTTATGTGGG CCAAGTTCCC GGAGGACTGA GAGAAGGCAT GGCCCTGTTC
    ATGCAGGGTG TTGTTCCTGA TAATGCTGAC CAATTTGAGA TCAATTTCAA GACTGGGCAG
    TCTGGCAGTG ATGACATTGC TTTCCACTTT AAACCCCAAA TGGGCCAGAA GGTGGTCATG
    AACAGCTTCA GGAATGGAGC ATGGGAAACT GAGGAAAGTG TCTCAGACAA CCCCTTTACA
    AAGGGACAAC ACTTTAAGAT GCTGACTGCC ATCACATCTG CAGGATATCA GGTGTACGTG
    AATGACAAAG AACTGTGCAC ATTCAAGCAC CGTCTACCAC TGGAAAGATT GTCAACATTA
    AATATCAGTG GAAATGTTGC CATCAACTCT CTTGGCTCCA TACAAAACTG GAGCACATCC
    TCACCTGTTG CTCAAGCCAG GTCAGCAATA CCCAGCCAGG GCAGCTCTCA TGGGAAATGG
    TCCAGCACAC ACTTGGAGAT CTCGCAACCA GTCCAACACC CTAAACTTAC TAAGGCGGTC
    CAGATTCCCG GAGGACTGAA AGAAGGCATG ATCTTGTACA TGAAGGGAGC TGTTCCTGCC
    AATGCTGACA GATTTGAAAT CAATTTCAAG ACTGGGCAGT CTGGTGATGA TGACACTGCT
    TTCCATTTCA ACCCTCGGCT GGATAAGAAC GTGGCCATGA ACAGCGTAAT AAATGGACAA
    TCTGGAACAG AAGAAATTAT CTCTGACAAC CCTTTTAAAA AGGGAGAACC ATTTGAGATG
    TTGACTATGA TCAAACCTGA AGGATATCAG GTGCAAGTGA ATGGCAAAGA ACAGTGCATA
    TTCAAGCACC GTACACCTCT TGAAAGAGTG TCAGCATTAA ATATCAGTGG AGACGTTGCT
    GTAAACAACT TGGAGATCTC ACAGTCAATC CAAAACCCAA ATATTCCTTA TGTGGACCAA
    ATTCCTGGAG GACTGAGAGA AGGCATGGCC ATGTTCATGC AGGGTGTTGT TCCTGATAAT
    GCTGACCAAT TTGAGATCAA TTTCAAGACT GGGCAGTCTG GCAGTGATGA CATTGCTTTC
    CACTTCAACC CCCGAATGGG CCAGAAGGTG ACCATGAACA GCTTCAGGAA TGGAGCATGG
    GAAACTGAGG AAAGTGTCTC AGACAACCCC TTTAAAAAAG GACAACATTT TAGTATGTTG
    ACTGCCATCA CATCTGCTGG ATATCAGGTG TACGTGAATG ACAAAGAACT GTGCACATTC
    AAGCACCGTC TACCACTGGA AAGATTGTCA ACATTAAATA TCAGTGGAAA TGTTGCCATC
    AACTCTCTTG GCTCCATACA AAACTGGAGC ACATCCTCAC TTGTTCCTCA AGCCAAGTCA
    ACAATCTCCA GCCTGGGCGG CTCACATGGG GAACAGTCTA CCACACACTT GGAGATCTCA
    CAACCAGTCC AACACCCTAG CATTCCTTAT GTTGGCCCCA TTTCAGGAGG ACTGAGAGAG
    GGCATGGCCG TGTACTTGCA GGGTGTTGTT CCTCCTAATG CTGACAAATT TGAGATCAAT
    TTCAAGACTG GGCAGTCTGG CAGTGATGAC ATTGCTTTCC ACTTTAACCC CCTAATGGGC
    CAGAATTTGG ACATGAACAG CTTCAGGAAT GGAGCATGGG AAACTCAGGA GAGTGTCTCC
    AACAACCCTT TTAAAAAGGG GGAAGCTTTT GAGATGTTCA TGGTCATCAA ATCTGAAGGA
    TATCAGGTAT ATGTAAACGA AAACGACCTG TACACATTCA AGCACCGTAT ACCACTGGAA
    AAAGTGTCCA TCTTGAATAT TGTTGGAGCG GTTTCTATGA ACATTTTTGG GTTCATTCAA
    AACTGGAGCA AATCCTCATT TACTACTAGA GTCAAGTCAA CAATCACCAG TCTGGGAAGC
    CCACAGTGGG ATCTGTCCAT AATACAATCA AAGGCCTTAC AAGCAGTGCA AAATCCCAAC
    CTTCCTTATT TGGGCCAAAT CCCTGGAGGA CTAAGAGAAG GCATGACCTT GTATGTGAAG
    GGAGTTGTTC CTAGTAATGG TGATCGGTTT TCAATCAACT TCAAGACCGG GTCTACTGAT
    AAGGATGACA TTGCTTTCCA CTTCAATCCC CGAATGGGCT CAAAGCTGGT CATGAACAGT
    ATGAAGAGCG GGAGATGGGG AGCGGAGGAA TACGTCTCTG AAAACCCGTG TAAAAAGGGA
    GATGCTTTTG AGTTCTACAT TCAGATCAAA GCTGAAGGAT ATCAGGTGCA ATTCAAAGAC
    CACAAGCAGA GCATGTTCAA ACACCGCATC CCTTTTGAAA GAGTGACTAC AATCAACGTT
    CTGGGGAATG TCTCTATCAG CTTTCTTGGA TTTGTCATGG GGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.