sec16a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sec16a
Entrez Gene ID	567075
Full Name	SEC16 homolog A, endoplasmic reticulum export factor
General protein information	Preferred Names SEC16 homolog A, endoplasmic reticulum export factor Names protein transport protein Sec16A zmp:0000000707
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009292712.3	XP_009290987.1	protein transport protein Sec16A isoform X2
XM_009292713.2	XP_009290988.1	protein transport protein Sec16A isoform X3
XM_009292712.2	XP_009290987.1	protein transport protein Sec16A isoform X2
XM_009292711.2	XP_009290986.1	protein transport protein Sec16A isoform X1
XM_009292713.3	XP_009290988.1	protein transport protein Sec16A isoform X3
XM_009292711.3	XP_009290986.1	protein transport protein Sec16A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SEC16A	NP_055681.1
Mus musculus (house mouse)	Sec16a	NP_694765.2
Canis lupus familiaris (dog)	SEC16A	XP_005625160.1
Danio rerio (zebrafish)	LOC567075	XP_695457.6
Bos taurus (cattle)	SEC16A	XP_002691705.1
Rattus norvegicus (Norway rat)	Sec16a	NP_001263346.1
Xenopus tropicalis (tropical clawed frog)	sec16a	XP_004916692.1
Pan troglodytes (chimpanzee)	SEC16A	XP_001171528.2
	SEC16A	XP_001117942.2
Gallus gallus (chicken)	SEC16A	XP_415419.4

Gene Ontology

The following sec16a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec16a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45072	XM_009292711.3 Latest version!	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45073	XM_009292712.3 Latest version!	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45074	XM_009292713.3 Latest version!	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
ODa45073	XM_009292712.2	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45074	XM_009292713.2	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45072	XM_009292711.2	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sec16a ( XM_009292711.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290986.1 Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X1, mRNA.

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CloneID	ODa45072
Clone ID Related Accession (Same CDS sequence)	XM_009292711.2 , XM_009292711.3
Accession Version	XM_009292711.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein transport protein Sec16A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAACAGG GATCTGGTGG CGGCTTGCTG 
ACCTGGCTGT TTCCAAAGCG GAAGAATGAA GCACATCTTC CAGACGACAA AAACAAATCT 
ATTGTGTGGG ATGAAAAGAA GCAGAAATGG GTGAATCTGG ATGAGCCAGA GGAAGAGAGT 
AAACCTCCTC CTCCCCCACC CACCGGCTTC CCCAAAGCTC CAATGAGCGC TCCAGCTATG 
GTACCTCCAG GAGTGGCCGG CCCACCATCA GGGCCTCCTG TGAACATGTT CTCCAGAAGA 
GCAGGCACGA GGAGTCGTTA TGTGGATGTG CTGAATCCTG GTCGTGGAGG AGCTAAAGCA 
GTGGCTTCAG TGCCTCCTCC TGCTGATCTG TTCGCTCCTC TCGCACCCAT GGCCATGCCA 
GCCAACCTCT TCACACCAGC TGCAGCCCCA GATGAGCAGC AGCCGATGGA GGGAAGTGTT 
CCAGACACCA GTGGAGAAAA CACACTGCAG ACAAGTGCCA CAGTGCCACA GATGTTTAAT 
CCGAATGCTG CTTTGCCTCC CGGCCCGGAG GGAACCCAGT CTGGAGAGCT GTCACGTTCT 
AGCTCAATGA GTTCTCTATC GCGAGAAGTG AGTCAGCATT TAAATCAGGC TCCTGCTCAA 
CAACCAGTGC AAGGAGCTCC GCCTGCAGGG GGTGTGACCT TTTATAACCC CTCCCAGTTT 
GCACAGTCTG TTCCCACAAA CCGTACTCGC CCTGGGCGCC TGGGACAGAG AGGATACCCA 
ACACCGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290986.1
CDS	194..6745
Translation	MQPPPRTTPPGASGPPPPSGGPNSFRRNRPHKHGAASAMTPAAPSTMPVTDPFAFGRQNFSSMAGSSQAPPTNSNPLPMQSPPLPGFNPTAPPQGPPPGPAQAASPGPPPRIFTPQPVPSRPGSSSIPPPAEAGYFSSQEPIPYIAQLPNPAPPLIPSTSHPPSTTPTPPVQSQAPFHSQPTPPLPSHWVPEHGSRPPSVQNYFQPTSDLPSQPFQAQAPHPGPSPLTAPPPVPSPLTAPPPLTSPLTAPPLTQSQNQFQSAQPNVSGPAATYQQNSHFPAHNYFSQSGGPSEPWFNQNPQDPSQRAYHVPYSEVPSDSGTLSMFFGGNDVENEETLTGEGRVNGIHQPLHSTTEFSGPYLNDSGNIRAGTGPSDSVENLECVPNQEVLPSEPPPSHAFEGGPNLETPDSAPRLARSASVSSSYSNVSHSSGHGAHSGGHVPRRQQVVGTFIQQESPRPPDHPPLHPSTGGYFEQIDSNPAAEASPTFPTPSPPKPVGVFQASANSSFEPVRSHGVGVRPAEVDRARVVMEKGGRDMQPGNLEHPPDNLETIYLSERRPSSRAQGARRPIESPATALWAQNDTASLSANILLAPAAPSLTATFVPAHPSEVIQPPEDGPLDLHPARPDPQPPSENLEIPPDSTPQASLGYASLLVTSPPEEALNQPVLIAPPSSNYSLISSQNLLQPSILPSPQETAAPVHPHPQSSLTNQMPQNSPPTNQPPLLFSQTPVNFSSSSAQGVLNLTREANDGQAARPNLSRAQSLKGDSQFLPPSATQAPPSSAASTNHNQPSNYQLLDFSMHQPQSTNQVTSVPAVSQSTHPAGLVNNTPGFYLQVTKDAQQGGQSDTDSLSHQQNSNPPLPSSNQLDNNQTLNSHPPPPPAQNQPPPQAPPTHPGAPPTGPGSAQPPPYPPQSGNSTTQPPQEPQRPPSSLGSQSGYGVPPAMPPGPGYPGYYPGAYPEYQDGRVPYPPQYPVDPRTQNYYQDDPYRRGDPRYGWYDGQNPGYREAERQPERPSSRTSQYSDRPSSRQGYQEDFSRSSRSAYDEYYANYYKRQYDAYGDRSRWYDPNAAYDPRYKAYYDQMYGWYYNQDGYRGGQAYYNQQYQNRGREGYDDPWRYYPGYDSSFDDDSRRRDPYADDFDRRSVHSEQSAHSVHSSHSRRSSFSSRSQQSQVYRSQPDLVSAAYDATGTTLPADYTYSQYPNPSDTVNYGQDPYNSADNTWSAPEQPPPRPLTPEKFSIPHRCARFGPAGQLILVQPNLPSAGQPTLVEIHSMETLLQDSPEQSDMRSFPGPLVKEETHKVDVMKFAQNKAQECLRNDDLIDKDSAYLIWEFIVLLCRQNGTVVGTDIADLLLKEHRSVWLPGKSPNEANLIDFNNEAIEHAEEDMGQMSLLSDTFMGVPENIGKDTERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDNRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTHTLDLDTRTISTMGDTLASKGLVDAAHFCYLMAQVGFGVYTKKSTKMVLISSNHSLPFLKFCSSEAIQRTESYEYAQSLGSQPLSLPNFQVFKFIYACRLAESGLCAQAFHYCEVISRTLLSLPEYYSPVFISQLIQMSLRLRFFDPQLKERPEQELFIEPDWLLHLRQLDGQIKEGVISLRTDRSTPQLYPCSTPSSEDQCSPPEPPALTPDPHNALMTSLVPPAAVQLMPPAPPTILQDGAVSLQQVPPPSESVPFYPVAPGPPQPNFVAPYQPEMQEQYMPMPPQIPQHMPPQMPTYNPAEMPPQMPQGMPGQMPPPMHGAERGSAPSSPQQFPQPSPTFANPPPPGKDFYDEMARLGPGRRSRTTSQSSMHMAPGRRSRTTSESSNHSGRERSSSFANQGSPPPPPIPEAPPQDVPERTKKDSPKKQGSGGGLLTWLFPKRKNEAHLPDDKNKSIVWDEKKQKWVNLDEPEEESKPPPPPPTGFPKAPMSAPAMVPPGVAGPPSGPPVNMFSRRAGTRSRYVDVLNPGRGGAKAVASVPPPADLFAPLAPMAMPANLFTPAAAPDEQQPMEGSVPDTSGENTLQTSATVPQMFNPNAALPPGPEGTQSGELSRSSSMSSLSREVSQHLNQAPAQQPVQGAPPAGGVTFYNPSQFAQSVPTNRTRPGRLGQRGYPTPK

Target ORF information:

RefSeq Version	XM_009292711.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292711.2

ORF Insert Sequence:

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAACAGG GATCTGGTGG CGGCTTGCTG 
ACCTGGCTGT TTCCAAAGCG GAAGAATGAA GCACATCTTC CAGACGACAA AAACAAATCT 
ATTGTGTGGG ATGAAAAGAA GCAGAAATGG GTGAATCTGG ATGAGCCAGA GGAAGAGAGT 
AAACCTCCTC CTCCCCCACC CACCGGCTTC CCCAAAGCTC CAATGAGCGC TCCAGCTATG 
GTACCTCCAG GAGTGGCCGG CCCACCATCA GGGCCTCCTG TGAACATGTT CTCCAGAAGA 
GCAGGCACGA GGAGTCGTTA TGTGGATGTG CTGAATCCTG GTCGTGGAGG AGCTAAAGCA 
GTGGCTTCAG TGCCTCCTCC TGCTGATCTG TTCGCTCCTC TCGCACCCAT GGCCATGCCA 
GCCAACCTCT TCACACCAGC TGCAGCCCCA GATGAGCAGC AGCCGATGGA GGGAAGTGTT 
CCAGACACCA GTGGAGAAAA CACACTGCAG ACAAGTGCCA CAGTGCCACA GATGTTTAAT 
CCGAATGCTG CTTTGCCTCC CGGCCCGGAG GGAACCCAGT CTGGAGAGCT GTCACGTTCT 
AGCTCAATGA GTTCTCTATC GCGAGAAGTG AGTCAGCATT TAAATCAGGC TCCTGCTCAA 
CAACCAGTGC AAGGAGCTCC GCCTGCAGGG GGTGTGACCT TTTATAACCC CTCCCAGTTT 
GCACAGTCTG TTCCCACAAA CCGTACTCGC CCTGGGCGCC TGGGACAGAG AGGATACCCA 
ACACCGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec16a ( XM_009292711.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290986.1 Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X1, mRNA.

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CloneID	ODa45072
Clone ID Related Accession (Same CDS sequence)	XM_009292711.2 , XM_009292711.3
Accession Version	XM_009292711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec16A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAACAGG GATCTGGTGG CGGCTTGCTG 
ACCTGGCTGT TTCCAAAGCG GAAGAATGAA GCACATCTTC CAGACGACAA AAACAAATCT 
ATTGTGTGGG ATGAAAAGAA GCAGAAATGG GTGAATCTGG ATGAGCCAGA GGAAGAGAGT 
AAACCTCCTC CTCCCCCACC CACCGGCTTC CCCAAAGCTC CAATGAGCGC TCCAGCTATG 
GTACCTCCAG GAGTGGCCGG CCCACCATCA GGGCCTCCTG TGAACATGTT CTCCAGAAGA 
GCAGGCACGA GGAGTCGTTA TGTGGATGTG CTGAATCCTG GTCGTGGAGG AGCTAAAGCA 
GTGGCTTCAG TGCCTCCTCC TGCTGATCTG TTCGCTCCTC TCGCACCCAT GGCCATGCCA 
GCCAACCTCT TCACACCAGC TGCAGCCCCA GATGAGCAGC AGCCGATGGA GGGAAGTGTT 
CCAGACACCA GTGGAGAAAA CACACTGCAG ACAAGTGCCA CAGTGCCACA GATGTTTAAT 
CCGAATGCTG CTTTGCCTCC CGGCCCGGAG GGAACCCAGT CTGGAGAGCT GTCACGTTCT 
AGCTCAATGA GTTCTCTATC GCGAGAAGTG AGTCAGCATT TAAATCAGGC TCCTGCTCAA 
CAACCAGTGC AAGGAGCTCC GCCTGCAGGG GGTGTGACCT TTTATAACCC CTCCCAGTTT 
GCACAGTCTG TTCCCACAAA CCGTACTCGC CCTGGGCGCC TGGGACAGAG AGGATACCCA 
ACACCGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290986.1
CDS	204..6755
Translation	MQPPPRTTPPGASGPPPPSGGPNSFRRNRPHKHGAASAMTPAAPSTMPVTDPFAFGRQNFSSMAGSSQAPPTNSNPLPMQSPPLPGFNPTAPPQGPPPGPAQAASPGPPPRIFTPQPVPSRPGSSSIPPPAEAGYFSSQEPIPYIAQLPNPAPPLIPSTSHPPSTTPTPPVQSQAPFHSQPTPPLPSHWVPEHGSRPPSVQNYFQPTSDLPSQPFQAQAPHPGPSPLTAPPPVPSPLTAPPPLTSPLTAPPLTQSQNQFQSAQPNVSGPAATYQQNSHFPAHNYFSQSGGPSEPWFNQNPQDPSQRAYHVPYSEVPSDSGTLSMFFGGNDVENEETLTGEGRVNGIHQPLHSTTEFSGPYLNDSGNIRAGTGPSDSVENLECVPNQEVLPSEPPPSHAFEGGPNLETPDSAPRLARSASVSSSYSNVSHSSGHGAHSGGHVPRRQQVVGTFIQQESPRPPDHPPLHPSTGGYFEQIDSNPAAEASPTFPTPSPPKPVGVFQASANSSFEPVRSHGVGVRPAEVDRARVVMEKGGRDMQPGNLEHPPDNLETIYLSERRPSSRAQGARRPIESPATALWAQNDTASLSANILLAPAAPSLTATFVPAHPSEVIQPPEDGPLDLHPARPDPQPPSENLEIPPDSTPQASLGYASLLVTSPPEEALNQPVLIAPPSSNYSLISSQNLLQPSILPSPQETAAPVHPHPQSSLTNQMPQNSPPTNQPPLLFSQTPVNFSSSSAQGVLNLTREANDGQAARPNLSRAQSLKGDSQFLPPSATQAPPSSAASTNHNQPSNYQLLDFSMHQPQSTNQVTSVPAVSQSTHPAGLVNNTPGFYLQVTKDAQQGGQSDTDSLSHQQNSNPPLPSSNQLDNNQTLNSHPPPPPAQNQPPPQAPPTHPGAPPTGPGSAQPPPYPPQSGNSTTQPPQEPQRPPSSLGSQSGYGVPPAMPPGPGYPGYYPGAYPEYQDGRVPYPPQYPVDPRTQNYYQDDPYRRGDPRYGWYDGQNPGYREAERQPERPSSRTSQYSDRPSSRQGYQEDFSRSSRSAYDEYYANYYKRQYDAYGDRSRWYDPNAAYDPRYKAYYDQMYGWYYNQDGYRGGQAYYNQQYQNRGREGYDDPWRYYPGYDSSFDDDSRRRDPYADDFDRRSVHSEQSAHSVHSSHSRRSSFSSRSQQSQVYRSQPDLVSAAYDATGTTLPADYTYSQYPNPSDTVNYGQDPYNSADNTWSAPEQPPPRPLTPEKFSIPHRCARFGPAGQLILVQPNLPSAGQPTLVEIHSMETLLQDSPEQSDMRSFPGPLVKEETHKVDVMKFAQNKAQECLRNDDLIDKDSAYLIWEFIVLLCRQNGTVVGTDIADLLLKEHRSVWLPGKSPNEANLIDFNNEAIEHAEEDMGQMSLLSDTFMGVPENIGKDTERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDNRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTHTLDLDTRTISTMGDTLASKGLVDAAHFCYLMAQVGFGVYTKKSTKMVLISSNHSLPFLKFCSSEAIQRTESYEYAQSLGSQPLSLPNFQVFKFIYACRLAESGLCAQAFHYCEVISRTLLSLPEYYSPVFISQLIQMSLRLRFFDPQLKERPEQELFIEPDWLLHLRQLDGQIKEGVISLRTDRSTPQLYPCSTPSSEDQCSPPEPPALTPDPHNALMTSLVPPAAVQLMPPAPPTILQDGAVSLQQVPPPSESVPFYPVAPGPPQPNFVAPYQPEMQEQYMPMPPQIPQHMPPQMPTYNPAEMPPQMPQGMPGQMPPPMHGAERGSAPSSPQQFPQPSPTFANPPPPGKDFYDEMARLGPGRRSRTTSQSSMHMAPGRRSRTTSESSNHSGRERSSSFANQGSPPPPPIPEAPPQDVPERTKKDSPKKQGSGGGLLTWLFPKRKNEAHLPDDKNKSIVWDEKKQKWVNLDEPEEESKPPPPPPTGFPKAPMSAPAMVPPGVAGPPSGPPVNMFSRRAGTRSRYVDVLNPGRGGAKAVASVPPPADLFAPLAPMAMPANLFTPAAAPDEQQPMEGSVPDTSGENTLQTSATVPQMFNPNAALPPGPEGTQSGELSRSSSMSSLSREVSQHLNQAPAQQPVQGAPPAGGVTFYNPSQFAQSVPTNRTRPGRLGQRGYPTPK

Target ORF information:

RefSeq Version	XM_009292711.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292711.3

ORF Insert Sequence:

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAACAGG GATCTGGTGG CGGCTTGCTG 
ACCTGGCTGT TTCCAAAGCG GAAGAATGAA GCACATCTTC CAGACGACAA AAACAAATCT 
ATTGTGTGGG ATGAAAAGAA GCAGAAATGG GTGAATCTGG ATGAGCCAGA GGAAGAGAGT 
AAACCTCCTC CTCCCCCACC CACCGGCTTC CCCAAAGCTC CAATGAGCGC TCCAGCTATG 
GTACCTCCAG GAGTGGCCGG CCCACCATCA GGGCCTCCTG TGAACATGTT CTCCAGAAGA 
GCAGGCACGA GGAGTCGTTA TGTGGATGTG CTGAATCCTG GTCGTGGAGG AGCTAAAGCA 
GTGGCTTCAG TGCCTCCTCC TGCTGATCTG TTCGCTCCTC TCGCACCCAT GGCCATGCCA 
GCCAACCTCT TCACACCAGC TGCAGCCCCA GATGAGCAGC AGCCGATGGA GGGAAGTGTT 
CCAGACACCA GTGGAGAAAA CACACTGCAG ACAAGTGCCA CAGTGCCACA GATGTTTAAT 
CCGAATGCTG CTTTGCCTCC CGGCCCGGAG GGAACCCAGT CTGGAGAGCT GTCACGTTCT 
AGCTCAATGA GTTCTCTATC GCGAGAAGTG AGTCAGCATT TAAATCAGGC TCCTGCTCAA 
CAACCAGTGC AAGGAGCTCC GCCTGCAGGG GGTGTGACCT TTTATAACCC CTCCCAGTTT 
GCACAGTCTG TTCCCACAAA CCGTACTCGC CCTGGGCGCC TGGGACAGAG AGGATACCCA 
ACACCGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec16a ( XM_009292712.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290987.1 Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X2, mRNA.

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CloneID	ODa45073
Clone ID Related Accession (Same CDS sequence)	XM_009292712.3 , XM_009292712.2
Accession Version	XM_009292712.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec16A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAAGGAT CTGGTGGCGG CTTGCTGACC 
TGGCTGTTTC CAAAGCGGAA GAATGAAGCA CATCTTCCAG ACGACAAAAA CAAATCTATT 
GTGTGGGATG AAAAGAAGCA GAAATGGGTG AATCTGGATG AGCCAGAGGA AGAGAGTAAA 
CCTCCTCCTC CCCCACCCAC CGGCTTCCCC AAAGCTCCAA TGAGCGCTCC AGCTATGGTA 
CCTCCAGGAG TGGCCGGCCC ACCATCAGGG CCTCCTGTGA ACATGTTCTC CAGAAGAGCA 
GGCACGAGGA GTCGTTATGT GGATGTGCTG AATCCTGGTC GTGGAGGAGC TAAAGCAGTG 
GCTTCAGTGC CTCCTCCTGC TGATCTGTTC GCTCCTCTCG CACCCATGGC CATGCCAGCC 
AACCTCTTCA CACCAGCTGC AGCCCCAGAT GAGCAGCAGC CGATGGAGGG AAGTGTTCCA 
GACACCAGTG GAGAAAACAC ACTGCAGACA AGTGCCACAG TGCCACAGAT GTTTAATCCG 
AATGCTGCTT TGCCTCCCGG CCCGGAGGGA ACCCAGTCTG GAGAGCTGTC ACGTTCTAGC 
TCAATGAGTT CTCTATCGCG AGAAGTGAGT CAGCATTTAA ATCAGGCTCC TGCTCAACAA 
CCAGTGCAAG GAGCTCCGCC TGCAGGGGGT GTGACCTTTT ATAACCCCTC CCAGTTTGCA 
CAGTCTGTTC CCACAAACCG TACTCGCCCT GGGCGCCTGG GACAGAGAGG ATACCCAACA 
CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290987.1
CDS	204..6752
Translation	MQPPPRTTPPGASGPPPPSGGPNSFRRNRPHKHGAASAMTPAAPSTMPVTDPFAFGRQNFSSMAGSSQAPPTNSNPLPMQSPPLPGFNPTAPPQGPPPGPAQAASPGPPPRIFTPQPVPSRPGSSSIPPPAEAGYFSSQEPIPYIAQLPNPAPPLIPSTSHPPSTTPTPPVQSQAPFHSQPTPPLPSHWVPEHGSRPPSVQNYFQPTSDLPSQPFQAQAPHPGPSPLTAPPPVPSPLTAPPPLTSPLTAPPLTQSQNQFQSAQPNVSGPAATYQQNSHFPAHNYFSQSGGPSEPWFNQNPQDPSQRAYHVPYSEVPSDSGTLSMFFGGNDVENEETLTGEGRVNGIHQPLHSTTEFSGPYLNDSGNIRAGTGPSDSVENLECVPNQEVLPSEPPPSHAFEGGPNLETPDSAPRLARSASVSSSYSNVSHSSGHGAHSGGHVPRRQQVVGTFIQQESPRPPDHPPLHPSTGGYFEQIDSNPAAEASPTFPTPSPPKPVGVFQASANSSFEPVRSHGVGVRPAEVDRARVVMEKGGRDMQPGNLEHPPDNLETIYLSERRPSSRAQGARRPIESPATALWAQNDTASLSANILLAPAAPSLTATFVPAHPSEVIQPPEDGPLDLHPARPDPQPPSENLEIPPDSTPQASLGYASLLVTSPPEEALNQPVLIAPPSSNYSLISSQNLLQPSILPSPQETAAPVHPHPQSSLTNQMPQNSPPTNQPPLLFSQTPVNFSSSSAQGVLNLTREANDGQAARPNLSRAQSLKGDSQFLPPSATQAPPSSAASTNHNQPSNYQLLDFSMHQPQSTNQVTSVPAVSQSTHPAGLVNNTPGFYLQVTKDAQQGGQSDTDSLSHQQNSNPPLPSSNQLDNNQTLNSHPPPPPAQNQPPPQAPPTHPGAPPTGPGSAQPPPYPPQSGNSTTQPPQEPQRPPSSLGSQSGYGVPPAMPPGPGYPGYYPGAYPEYQDGRVPYPPQYPVDPRTQNYYQDDPYRRGDPRYGWYDGQNPGYREAERQPERPSSRTSQYSDRPSSRQGYQEDFSRSSRSAYDEYYANYYKRQYDAYGDRSRWYDPNAAYDPRYKAYYDQMYGWYYNQDGYRGGQAYYNQQYQNRGREGYDDPWRYYPGYDSSFDDDSRRRDPYADDFDRRSVHSEQSAHSVHSSHSRRSSFSSRSQQSQVYRSQPDLVSAAYDATGTTLPADYTYSQYPNPSDTVNYGQDPYNSADNTWSAPEQPPPRPLTPEKFSIPHRCARFGPAGQLILVQPNLPSAGQPTLVEIHSMETLLQDSPEQSDMRSFPGPLVKEETHKVDVMKFAQNKAQECLRNDDLIDKDSAYLIWEFIVLLCRQNGTVVGTDIADLLLKEHRSVWLPGKSPNEANLIDFNNEAIEHAEEDMGQMSLLSDTFMGVPENIGKDTERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDNRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTHTLDLDTRTISTMGDTLASKGLVDAAHFCYLMAQVGFGVYTKKSTKMVLISSNHSLPFLKFCSSEAIQRTESYEYAQSLGSQPLSLPNFQVFKFIYACRLAESGLCAQAFHYCEVISRTLLSLPEYYSPVFISQLIQMSLRLRFFDPQLKERPEQELFIEPDWLLHLRQLDGQIKEGVISLRTDRSTPQLYPCSTPSSEDQCSPPEPPALTPDPHNALMTSLVPPAAVQLMPPAPPTILQDGAVSLQQVPPPSESVPFYPVAPGPPQPNFVAPYQPEMQEQYMPMPPQIPQHMPPQMPTYNPAEMPPQMPQGMPGQMPPPMHGAERGSAPSSPQQFPQPSPTFANPPPPGKDFYDEMARLGPGRRSRTTSQSSMHMAPGRRSRTTSESSNHSGRERSSSFANQGSPPPPPIPEAPPQDVPERTKKDSPKKGSGGGLLTWLFPKRKNEAHLPDDKNKSIVWDEKKQKWVNLDEPEEESKPPPPPPTGFPKAPMSAPAMVPPGVAGPPSGPPVNMFSRRAGTRSRYVDVLNPGRGGAKAVASVPPPADLFAPLAPMAMPANLFTPAAAPDEQQPMEGSVPDTSGENTLQTSATVPQMFNPNAALPPGPEGTQSGELSRSSSMSSLSREVSQHLNQAPAQQPVQGAPPAGGVTFYNPSQFAQSVPTNRTRPGRLGQRGYPTPK

Target ORF information:

RefSeq Version	XM_009292712.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292712.3

ORF Insert Sequence:

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAAGGAT CTGGTGGCGG CTTGCTGACC 
TGGCTGTTTC CAAAGCGGAA GAATGAAGCA CATCTTCCAG ACGACAAAAA CAAATCTATT 
GTGTGGGATG AAAAGAAGCA GAAATGGGTG AATCTGGATG AGCCAGAGGA AGAGAGTAAA 
CCTCCTCCTC CCCCACCCAC CGGCTTCCCC AAAGCTCCAA TGAGCGCTCC AGCTATGGTA 
CCTCCAGGAG TGGCCGGCCC ACCATCAGGG CCTCCTGTGA ACATGTTCTC CAGAAGAGCA 
GGCACGAGGA GTCGTTATGT GGATGTGCTG AATCCTGGTC GTGGAGGAGC TAAAGCAGTG 
GCTTCAGTGC CTCCTCCTGC TGATCTGTTC GCTCCTCTCG CACCCATGGC CATGCCAGCC 
AACCTCTTCA CACCAGCTGC AGCCCCAGAT GAGCAGCAGC CGATGGAGGG AAGTGTTCCA 
GACACCAGTG GAGAAAACAC ACTGCAGACA AGTGCCACAG TGCCACAGAT GTTTAATCCG 
AATGCTGCTT TGCCTCCCGG CCCGGAGGGA ACCCAGTCTG GAGAGCTGTC ACGTTCTAGC 
TCAATGAGTT CTCTATCGCG AGAAGTGAGT CAGCATTTAA ATCAGGCTCC TGCTCAACAA 
CCAGTGCAAG GAGCTCCGCC TGCAGGGGGT GTGACCTTTT ATAACCCCTC CCAGTTTGCA 
CAGTCTGTTC CCACAAACCG TACTCGCCCT GGGCGCCTGG GACAGAGAGG ATACCCAACA 
CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec16a ( XM_009292712.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290987.1 Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X2, mRNA.

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CloneID	ODa45073
Clone ID Related Accession (Same CDS sequence)	XM_009292712.3 , XM_009292712.2
Accession Version	XM_009292712.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein transport protein Sec16A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAAGGAT CTGGTGGCGG CTTGCTGACC 
TGGCTGTTTC CAAAGCGGAA GAATGAAGCA CATCTTCCAG ACGACAAAAA CAAATCTATT 
GTGTGGGATG AAAAGAAGCA GAAATGGGTG AATCTGGATG AGCCAGAGGA AGAGAGTAAA 
CCTCCTCCTC CCCCACCCAC CGGCTTCCCC AAAGCTCCAA TGAGCGCTCC AGCTATGGTA 
CCTCCAGGAG TGGCCGGCCC ACCATCAGGG CCTCCTGTGA ACATGTTCTC CAGAAGAGCA 
GGCACGAGGA GTCGTTATGT GGATGTGCTG AATCCTGGTC GTGGAGGAGC TAAAGCAGTG 
GCTTCAGTGC CTCCTCCTGC TGATCTGTTC GCTCCTCTCG CACCCATGGC CATGCCAGCC 
AACCTCTTCA CACCAGCTGC AGCCCCAGAT GAGCAGCAGC CGATGGAGGG AAGTGTTCCA 
GACACCAGTG GAGAAAACAC ACTGCAGACA AGTGCCACAG TGCCACAGAT GTTTAATCCG 
AATGCTGCTT TGCCTCCCGG CCCGGAGGGA ACCCAGTCTG GAGAGCTGTC ACGTTCTAGC 
TCAATGAGTT CTCTATCGCG AGAAGTGAGT CAGCATTTAA ATCAGGCTCC TGCTCAACAA 
CCAGTGCAAG GAGCTCCGCC TGCAGGGGGT GTGACCTTTT ATAACCCCTC CCAGTTTGCA 
CAGTCTGTTC CCACAAACCG TACTCGCCCT GGGCGCCTGG GACAGAGAGG ATACCCAACA 
CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290987.1
CDS	194..6742
Translation	MQPPPRTTPPGASGPPPPSGGPNSFRRNRPHKHGAASAMTPAAPSTMPVTDPFAFGRQNFSSMAGSSQAPPTNSNPLPMQSPPLPGFNPTAPPQGPPPGPAQAASPGPPPRIFTPQPVPSRPGSSSIPPPAEAGYFSSQEPIPYIAQLPNPAPPLIPSTSHPPSTTPTPPVQSQAPFHSQPTPPLPSHWVPEHGSRPPSVQNYFQPTSDLPSQPFQAQAPHPGPSPLTAPPPVPSPLTAPPPLTSPLTAPPLTQSQNQFQSAQPNVSGPAATYQQNSHFPAHNYFSQSGGPSEPWFNQNPQDPSQRAYHVPYSEVPSDSGTLSMFFGGNDVENEETLTGEGRVNGIHQPLHSTTEFSGPYLNDSGNIRAGTGPSDSVENLECVPNQEVLPSEPPPSHAFEGGPNLETPDSAPRLARSASVSSSYSNVSHSSGHGAHSGGHVPRRQQVVGTFIQQESPRPPDHPPLHPSTGGYFEQIDSNPAAEASPTFPTPSPPKPVGVFQASANSSFEPVRSHGVGVRPAEVDRARVVMEKGGRDMQPGNLEHPPDNLETIYLSERRPSSRAQGARRPIESPATALWAQNDTASLSANILLAPAAPSLTATFVPAHPSEVIQPPEDGPLDLHPARPDPQPPSENLEIPPDSTPQASLGYASLLVTSPPEEALNQPVLIAPPSSNYSLISSQNLLQPSILPSPQETAAPVHPHPQSSLTNQMPQNSPPTNQPPLLFSQTPVNFSSSSAQGVLNLTREANDGQAARPNLSRAQSLKGDSQFLPPSATQAPPSSAASTNHNQPSNYQLLDFSMHQPQSTNQVTSVPAVSQSTHPAGLVNNTPGFYLQVTKDAQQGGQSDTDSLSHQQNSNPPLPSSNQLDNNQTLNSHPPPPPAQNQPPPQAPPTHPGAPPTGPGSAQPPPYPPQSGNSTTQPPQEPQRPPSSLGSQSGYGVPPAMPPGPGYPGYYPGAYPEYQDGRVPYPPQYPVDPRTQNYYQDDPYRRGDPRYGWYDGQNPGYREAERQPERPSSRTSQYSDRPSSRQGYQEDFSRSSRSAYDEYYANYYKRQYDAYGDRSRWYDPNAAYDPRYKAYYDQMYGWYYNQDGYRGGQAYYNQQYQNRGREGYDDPWRYYPGYDSSFDDDSRRRDPYADDFDRRSVHSEQSAHSVHSSHSRRSSFSSRSQQSQVYRSQPDLVSAAYDATGTTLPADYTYSQYPNPSDTVNYGQDPYNSADNTWSAPEQPPPRPLTPEKFSIPHRCARFGPAGQLILVQPNLPSAGQPTLVEIHSMETLLQDSPEQSDMRSFPGPLVKEETHKVDVMKFAQNKAQECLRNDDLIDKDSAYLIWEFIVLLCRQNGTVVGTDIADLLLKEHRSVWLPGKSPNEANLIDFNNEAIEHAEEDMGQMSLLSDTFMGVPENIGKDTERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDNRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTHTLDLDTRTISTMGDTLASKGLVDAAHFCYLMAQVGFGVYTKKSTKMVLISSNHSLPFLKFCSSEAIQRTESYEYAQSLGSQPLSLPNFQVFKFIYACRLAESGLCAQAFHYCEVISRTLLSLPEYYSPVFISQLIQMSLRLRFFDPQLKERPEQELFIEPDWLLHLRQLDGQIKEGVISLRTDRSTPQLYPCSTPSSEDQCSPPEPPALTPDPHNALMTSLVPPAAVQLMPPAPPTILQDGAVSLQQVPPPSESVPFYPVAPGPPQPNFVAPYQPEMQEQYMPMPPQIPQHMPPQMPTYNPAEMPPQMPQGMPGQMPPPMHGAERGSAPSSPQQFPQPSPTFANPPPPGKDFYDEMARLGPGRRSRTTSQSSMHMAPGRRSRTTSESSNHSGRERSSSFANQGSPPPPPIPEAPPQDVPERTKKDSPKKGSGGGLLTWLFPKRKNEAHLPDDKNKSIVWDEKKQKWVNLDEPEEESKPPPPPPTGFPKAPMSAPAMVPPGVAGPPSGPPVNMFSRRAGTRSRYVDVLNPGRGGAKAVASVPPPADLFAPLAPMAMPANLFTPAAAPDEQQPMEGSVPDTSGENTLQTSATVPQMFNPNAALPPGPEGTQSGELSRSSSMSSLSREVSQHLNQAPAQQPVQGAPPAGGVTFYNPSQFAQSVPTNRTRPGRLGQRGYPTPK

Target ORF information:

RefSeq Version	XM_009292712.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292712.2

ORF Insert Sequence:

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAAGGAT CTGGTGGCGG CTTGCTGACC 
TGGCTGTTTC CAAAGCGGAA GAATGAAGCA CATCTTCCAG ACGACAAAAA CAAATCTATT 
GTGTGGGATG AAAAGAAGCA GAAATGGGTG AATCTGGATG AGCCAGAGGA AGAGAGTAAA 
CCTCCTCCTC CCCCACCCAC CGGCTTCCCC AAAGCTCCAA TGAGCGCTCC AGCTATGGTA 
CCTCCAGGAG TGGCCGGCCC ACCATCAGGG CCTCCTGTGA ACATGTTCTC CAGAAGAGCA 
GGCACGAGGA GTCGTTATGT GGATGTGCTG AATCCTGGTC GTGGAGGAGC TAAAGCAGTG 
GCTTCAGTGC CTCCTCCTGC TGATCTGTTC GCTCCTCTCG CACCCATGGC CATGCCAGCC 
AACCTCTTCA CACCAGCTGC AGCCCCAGAT GAGCAGCAGC CGATGGAGGG AAGTGTTCCA 
GACACCAGTG GAGAAAACAC ACTGCAGACA AGTGCCACAG TGCCACAGAT GTTTAATCCG 
AATGCTGCTT TGCCTCCCGG CCCGGAGGGA ACCCAGTCTG GAGAGCTGTC ACGTTCTAGC 
TCAATGAGTT CTCTATCGCG AGAAGTGAGT CAGCATTTAA ATCAGGCTCC TGCTCAACAA 
CCAGTGCAAG GAGCTCCGCC TGCAGGGGGT GTGACCTTTT ATAACCCCTC CCAGTTTGCA 
CAGTCTGTTC CCACAAACCG TACTCGCCCT GGGCGCCTGG GACAGAGAGG ATACCCAACA 
CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec16a ( XM_009292713.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290988.1 Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X3, mRNA.

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CloneID	ODa45074
Clone ID Related Accession (Same CDS sequence)	XM_009292713.2 , XM_009292713.3
Accession Version	XM_009292713.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein transport protein Sec16A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAACAGG GATCTGGTGG CGGCTTGCTG 
ACCTGGCTGT TTCCAAAGCG GAAGAATGAA GCACATCTTC CAGACGACAA AAACAAATCT 
ATTGTGTGGG ATGAAAAGAA GCAGAAATGG GTGAATCTGG ATGAGCCAGA GGAAGAGAGT 
AAACCTCCTC CTCCCCCACC CACCGGCTTC CCCAAAGCTC CAATGAGCGC TCCAGCTATG 
GTACCTCCAG GAGTGGCCGG CCCACCATCA GGGCCTCCTG TGAACATGTT CTCCAGAAGA 
GCAGGCACGA GGAGTCGTTA TGTGGATGTG CTGAATCCTG GTCGTGGAGG AGCTAAAGCA 
GTGGCTTCAG TGCCTCCTCC TGCTGATCTG TTCGCTCCTC TCGCACCCAT GGCCATGCCA 
GCCAACCTCT TCACACCAGC TGCAGCCCCA GATGAGCAGC AGCCGATGGA GGGAAGTGTT 
CCAGACACCA GTGGAGAAAA CACACTGCAG ACAAGTGCCA CAGTGCCACA GATGTTTAAT 
CCGAATGCTG CTTTGCCTCC CGGCCCGGAG GGAACCCAGT CTGGAGAGGC TCCTGCTCAA 
CAACCAGTGC AAGGAGCTCC GCCTGCAGGG GGTGTGACCT TTTATAACCC CTCCCAGTTT 
GCACAGTCTG TTCCCACAAA CCGTACTCGC CCTGGGCGCC TGGGACAGAG AGGATACCCA 
ACACCGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290988.1
CDS	194..6685
Translation	MQPPPRTTPPGASGPPPPSGGPNSFRRNRPHKHGAASAMTPAAPSTMPVTDPFAFGRQNFSSMAGSSQAPPTNSNPLPMQSPPLPGFNPTAPPQGPPPGPAQAASPGPPPRIFTPQPVPSRPGSSSIPPPAEAGYFSSQEPIPYIAQLPNPAPPLIPSTSHPPSTTPTPPVQSQAPFHSQPTPPLPSHWVPEHGSRPPSVQNYFQPTSDLPSQPFQAQAPHPGPSPLTAPPPVPSPLTAPPPLTSPLTAPPLTQSQNQFQSAQPNVSGPAATYQQNSHFPAHNYFSQSGGPSEPWFNQNPQDPSQRAYHVPYSEVPSDSGTLSMFFGGNDVENEETLTGEGRVNGIHQPLHSTTEFSGPYLNDSGNIRAGTGPSDSVENLECVPNQEVLPSEPPPSHAFEGGPNLETPDSAPRLARSASVSSSYSNVSHSSGHGAHSGGHVPRRQQVVGTFIQQESPRPPDHPPLHPSTGGYFEQIDSNPAAEASPTFPTPSPPKPVGVFQASANSSFEPVRSHGVGVRPAEVDRARVVMEKGGRDMQPGNLEHPPDNLETIYLSERRPSSRAQGARRPIESPATALWAQNDTASLSANILLAPAAPSLTATFVPAHPSEVIQPPEDGPLDLHPARPDPQPPSENLEIPPDSTPQASLGYASLLVTSPPEEALNQPVLIAPPSSNYSLISSQNLLQPSILPSPQETAAPVHPHPQSSLTNQMPQNSPPTNQPPLLFSQTPVNFSSSSAQGVLNLTREANDGQAARPNLSRAQSLKGDSQFLPPSATQAPPSSAASTNHNQPSNYQLLDFSMHQPQSTNQVTSVPAVSQSTHPAGLVNNTPGFYLQVTKDAQQGGQSDTDSLSHQQNSNPPLPSSNQLDNNQTLNSHPPPPPAQNQPPPQAPPTHPGAPPTGPGSAQPPPYPPQSGNSTTQPPQEPQRPPSSLGSQSGYGVPPAMPPGPGYPGYYPGAYPEYQDGRVPYPPQYPVDPRTQNYYQDDPYRRGDPRYGWYDGQNPGYREAERQPERPSSRTSQYSDRPSSRQGYQEDFSRSSRSAYDEYYANYYKRQYDAYGDRSRWYDPNAAYDPRYKAYYDQMYGWYYNQDGYRGGQAYYNQQYQNRGREGYDDPWRYYPGYDSSFDDDSRRRDPYADDFDRRSVHSEQSAHSVHSSHSRRSSFSSRSQQSQVYRSQPDLVSAAYDATGTTLPADYTYSQYPNPSDTVNYGQDPYNSADNTWSAPEQPPPRPLTPEKFSIPHRCARFGPAGQLILVQPNLPSAGQPTLVEIHSMETLLQDSPEQSDMRSFPGPLVKEETHKVDVMKFAQNKAQECLRNDDLIDKDSAYLIWEFIVLLCRQNGTVVGTDIADLLLKEHRSVWLPGKSPNEANLIDFNNEAIEHAEEDMGQMSLLSDTFMGVPENIGKDTERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDNRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTHTLDLDTRTISTMGDTLASKGLVDAAHFCYLMAQVGFGVYTKKSTKMVLISSNHSLPFLKFCSSEAIQRTESYEYAQSLGSQPLSLPNFQVFKFIYACRLAESGLCAQAFHYCEVISRTLLSLPEYYSPVFISQLIQMSLRLRFFDPQLKERPEQELFIEPDWLLHLRQLDGQIKEGVISLRTDRSTPQLYPCSTPSSEDQCSPPEPPALTPDPHNALMTSLVPPAAVQLMPPAPPTILQDGAVSLQQVPPPSESVPFYPVAPGPPQPNFVAPYQPEMQEQYMPMPPQIPQHMPPQMPTYNPAEMPPQMPQGMPGQMPPPMHGAERGSAPSSPQQFPQPSPTFANPPPPGKDFYDEMARLGPGRRSRTTSQSSMHMAPGRRSRTTSESSNHSGRERSSSFANQGSPPPPPIPEAPPQDVPERTKKDSPKKQGSGGGLLTWLFPKRKNEAHLPDDKNKSIVWDEKKQKWVNLDEPEEESKPPPPPPTGFPKAPMSAPAMVPPGVAGPPSGPPVNMFSRRAGTRSRYVDVLNPGRGGAKAVASVPPPADLFAPLAPMAMPANLFTPAAAPDEQQPMEGSVPDTSGENTLQTSATVPQMFNPNAALPPGPEGTQSGEAPAQQPVQGAPPAGGVTFYNPSQFAQSVPTNRTRPGRLGQRGYPTPK

Target ORF information:

RefSeq Version	XM_009292713.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292713.2

ORF Insert Sequence:

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAACAGG GATCTGGTGG CGGCTTGCTG 
ACCTGGCTGT TTCCAAAGCG GAAGAATGAA GCACATCTTC CAGACGACAA AAACAAATCT 
ATTGTGTGGG ATGAAAAGAA GCAGAAATGG GTGAATCTGG ATGAGCCAGA GGAAGAGAGT 
AAACCTCCTC CTCCCCCACC CACCGGCTTC CCCAAAGCTC CAATGAGCGC TCCAGCTATG 
GTACCTCCAG GAGTGGCCGG CCCACCATCA GGGCCTCCTG TGAACATGTT CTCCAGAAGA 
GCAGGCACGA GGAGTCGTTA TGTGGATGTG CTGAATCCTG GTCGTGGAGG AGCTAAAGCA 
GTGGCTTCAG TGCCTCCTCC TGCTGATCTG TTCGCTCCTC TCGCACCCAT GGCCATGCCA 
GCCAACCTCT TCACACCAGC TGCAGCCCCA GATGAGCAGC AGCCGATGGA GGGAAGTGTT 
CCAGACACCA GTGGAGAAAA CACACTGCAG ACAAGTGCCA CAGTGCCACA GATGTTTAAT 
CCGAATGCTG CTTTGCCTCC CGGCCCGGAG GGAACCCAGT CTGGAGAGGC TCCTGCTCAA 
CAACCAGTGC AAGGAGCTCC GCCTGCAGGG GGTGTGACCT TTTATAACCC CTCCCAGTTT 
GCACAGTCTG TTCCCACAAA CCGTACTCGC CCTGGGCGCC TGGGACAGAG AGGATACCCA 
ACACCGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec16a ( XM_009292713.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290988.1 Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X3, mRNA.

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CloneID	ODa45074
Clone ID Related Accession (Same CDS sequence)	XM_009292713.2 , XM_009292713.3
Accession Version	XM_009292713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec16A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAACAGG GATCTGGTGG CGGCTTGCTG 
ACCTGGCTGT TTCCAAAGCG GAAGAATGAA GCACATCTTC CAGACGACAA AAACAAATCT 
ATTGTGTGGG ATGAAAAGAA GCAGAAATGG GTGAATCTGG ATGAGCCAGA GGAAGAGAGT 
AAACCTCCTC CTCCCCCACC CACCGGCTTC CCCAAAGCTC CAATGAGCGC TCCAGCTATG 
GTACCTCCAG GAGTGGCCGG CCCACCATCA GGGCCTCCTG TGAACATGTT CTCCAGAAGA 
GCAGGCACGA GGAGTCGTTA TGTGGATGTG CTGAATCCTG GTCGTGGAGG AGCTAAAGCA 
GTGGCTTCAG TGCCTCCTCC TGCTGATCTG TTCGCTCCTC TCGCACCCAT GGCCATGCCA 
GCCAACCTCT TCACACCAGC TGCAGCCCCA GATGAGCAGC AGCCGATGGA GGGAAGTGTT 
CCAGACACCA GTGGAGAAAA CACACTGCAG ACAAGTGCCA CAGTGCCACA GATGTTTAAT 
CCGAATGCTG CTTTGCCTCC CGGCCCGGAG GGAACCCAGT CTGGAGAGGC TCCTGCTCAA 
CAACCAGTGC AAGGAGCTCC GCCTGCAGGG GGTGTGACCT TTTATAACCC CTCCCAGTTT 
GCACAGTCTG TTCCCACAAA CCGTACTCGC CCTGGGCGCC TGGGACAGAG AGGATACCCA 
ACACCGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290988.1
CDS	204..6695
Translation	MQPPPRTTPPGASGPPPPSGGPNSFRRNRPHKHGAASAMTPAAPSTMPVTDPFAFGRQNFSSMAGSSQAPPTNSNPLPMQSPPLPGFNPTAPPQGPPPGPAQAASPGPPPRIFTPQPVPSRPGSSSIPPPAEAGYFSSQEPIPYIAQLPNPAPPLIPSTSHPPSTTPTPPVQSQAPFHSQPTPPLPSHWVPEHGSRPPSVQNYFQPTSDLPSQPFQAQAPHPGPSPLTAPPPVPSPLTAPPPLTSPLTAPPLTQSQNQFQSAQPNVSGPAATYQQNSHFPAHNYFSQSGGPSEPWFNQNPQDPSQRAYHVPYSEVPSDSGTLSMFFGGNDVENEETLTGEGRVNGIHQPLHSTTEFSGPYLNDSGNIRAGTGPSDSVENLECVPNQEVLPSEPPPSHAFEGGPNLETPDSAPRLARSASVSSSYSNVSHSSGHGAHSGGHVPRRQQVVGTFIQQESPRPPDHPPLHPSTGGYFEQIDSNPAAEASPTFPTPSPPKPVGVFQASANSSFEPVRSHGVGVRPAEVDRARVVMEKGGRDMQPGNLEHPPDNLETIYLSERRPSSRAQGARRPIESPATALWAQNDTASLSANILLAPAAPSLTATFVPAHPSEVIQPPEDGPLDLHPARPDPQPPSENLEIPPDSTPQASLGYASLLVTSPPEEALNQPVLIAPPSSNYSLISSQNLLQPSILPSPQETAAPVHPHPQSSLTNQMPQNSPPTNQPPLLFSQTPVNFSSSSAQGVLNLTREANDGQAARPNLSRAQSLKGDSQFLPPSATQAPPSSAASTNHNQPSNYQLLDFSMHQPQSTNQVTSVPAVSQSTHPAGLVNNTPGFYLQVTKDAQQGGQSDTDSLSHQQNSNPPLPSSNQLDNNQTLNSHPPPPPAQNQPPPQAPPTHPGAPPTGPGSAQPPPYPPQSGNSTTQPPQEPQRPPSSLGSQSGYGVPPAMPPGPGYPGYYPGAYPEYQDGRVPYPPQYPVDPRTQNYYQDDPYRRGDPRYGWYDGQNPGYREAERQPERPSSRTSQYSDRPSSRQGYQEDFSRSSRSAYDEYYANYYKRQYDAYGDRSRWYDPNAAYDPRYKAYYDQMYGWYYNQDGYRGGQAYYNQQYQNRGREGYDDPWRYYPGYDSSFDDDSRRRDPYADDFDRRSVHSEQSAHSVHSSHSRRSSFSSRSQQSQVYRSQPDLVSAAYDATGTTLPADYTYSQYPNPSDTVNYGQDPYNSADNTWSAPEQPPPRPLTPEKFSIPHRCARFGPAGQLILVQPNLPSAGQPTLVEIHSMETLLQDSPEQSDMRSFPGPLVKEETHKVDVMKFAQNKAQECLRNDDLIDKDSAYLIWEFIVLLCRQNGTVVGTDIADLLLKEHRSVWLPGKSPNEANLIDFNNEAIEHAEEDMGQMSLLSDTFMGVPENIGKDTERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDNRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTHTLDLDTRTISTMGDTLASKGLVDAAHFCYLMAQVGFGVYTKKSTKMVLISSNHSLPFLKFCSSEAIQRTESYEYAQSLGSQPLSLPNFQVFKFIYACRLAESGLCAQAFHYCEVISRTLLSLPEYYSPVFISQLIQMSLRLRFFDPQLKERPEQELFIEPDWLLHLRQLDGQIKEGVISLRTDRSTPQLYPCSTPSSEDQCSPPEPPALTPDPHNALMTSLVPPAAVQLMPPAPPTILQDGAVSLQQVPPPSESVPFYPVAPGPPQPNFVAPYQPEMQEQYMPMPPQIPQHMPPQMPTYNPAEMPPQMPQGMPGQMPPPMHGAERGSAPSSPQQFPQPSPTFANPPPPGKDFYDEMARLGPGRRSRTTSQSSMHMAPGRRSRTTSESSNHSGRERSSSFANQGSPPPPPIPEAPPQDVPERTKKDSPKKQGSGGGLLTWLFPKRKNEAHLPDDKNKSIVWDEKKQKWVNLDEPEEESKPPPPPPTGFPKAPMSAPAMVPPGVAGPPSGPPVNMFSRRAGTRSRYVDVLNPGRGGAKAVASVPPPADLFAPLAPMAMPANLFTPAAAPDEQQPMEGSVPDTSGENTLQTSATVPQMFNPNAALPPGPEGTQSGEAPAQQPVQGAPPAGGVTFYNPSQFAQSVPTNRTRPGRLGQRGYPTPK

Target ORF information:

RefSeq Version	XM_009292713.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC16 homolog A, endoplasmic reticulum export factor (sec16a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292713.3

ORF Insert Sequence:

ATGCAGCCCC CTCCTCGAAC AACTCCTCCT GGAGCAAGTG GACCTCCACC TCCTTCTGGA 
GGCCCCAATT CATTCAGAAG AAACAGACCC CATAAACATG GGGCGGCGTC TGCTATGACA 
CCGGCTGCCC CTTCCACAAT GCCAGTGACT GATCCTTTTG CCTTTGGGAG ACAAAATTTT 
TCATCTATGG CAGGGAGCAG CCAAGCTCCA CCCACAAACA GCAACCCACT TCCTATGCAA 
AGTCCTCCCC TGCCAGGGTT CAACCCAACA GCTCCACCAC AAGGGCCACC ACCAGGACCC 
GCACAGGCAG CCTCACCTGG CCCACCACCA AGAATTTTTA CACCACAGCC CGTTCCATCA 
CGGCCTGGAT CAAGCTCTAT CCCTCCTCCT GCAGAAGCAG GATACTTCAG TTCCCAAGAA 
CCAATCCCCT ATATTGCACA ATTGCCTAAC CCTGCTCCTC CTTTAATTCC TAGTACATCA 
CATCCTCCCT CCACTACCCC AACACCTCCA GTTCAGTCCC AGGCTCCTTT TCATTCCCAG 
CCAACCCCTC CCTTACCTTC ACACTGGGTG CCTGAACATG GAAGCAGACC ACCTTCTGTT 
CAAAACTACT TTCAGCCAAC TAGTGATCTC CCATCTCAGC CTTTCCAAGC TCAAGCCCCA 
CACCCTGGAC CCTCTCCTCT TACAGCCCCA CCTCCTGTAC CTTCTCCCCT TACAGCCCCA 
CCCCCTTTAA CTTCTCCTCT CACAGCCCCA CCTTTGACAC AATCTCAAAA TCAATTTCAG 
TCAGCTCAAC CAAATGTTAG TGGCCCTGCT GCAACTTATC AACAGAACTC CCACTTCCCT 
GCTCATAACT ATTTCAGTCA GTCAGGCGGA CCCTCTGAGC CATGGTTCAA TCAGAACCCA 
CAGGATCCAT CTCAGCGTGC CTACCATGTC CCCTACTCTG AAGTCCCTAG TGACTCAGGA 
ACTCTGTCAA TGTTTTTTGG GGGAAATGAT GTTGAAAATG AAGAAACTCT AACAGGGGAG 
GGGCGTGTAA ATGGAATTCA TCAACCTCTC CATTCAACAA CAGAATTCTC TGGTCCCTAT 
TTGAATGACA GTGGTAACAT ACGAGCTGGG ACAGGACCAA GTGATTCAGT GGAGAATCTG 
GAATGTGTTC CAAACCAGGA AGTTCTGCCT AGTGAGCCAC CTCCAAGTCA TGCCTTTGAG 
GGGGGACCTA ACTTGGAGAC ACCAGACTCT GCTCCTCGCC TTGCCCGCTC TGCAAGTGTC 
TCATCCAGCT ACAGTAATGT CAGTCACAGT AGTGGACACG GTGCTCACAG TGGGGGTCAT 
GTCCCTCGTA GACAACAAGT AGTGGGAACA TTTATCCAAC AGGAGAGTCC TCGTCCACCT 
GATCACCCTC CACTGCATCC CTCAACTGGT GGATACTTTG AACAAATAGA CTCTAACCCA 
GCTGCAGAGG CAAGTCCTAC TTTCCCAACT CCTAGTCCAC CTAAACCAGT TGGTGTGTTC 
CAGGCAAGTG CAAATTCCTC ATTTGAGCCT GTTCGTTCAC ATGGTGTAGG AGTTCGACCT 
GCTGAAGTTG ATCGTGCACG TGTGGTCATG GAAAAGGGTG GACGTGACAT GCAGCCTGGC 
AATTTAGAGC ATCCTCCAGA TAACTTGGAG ACTATTTACT TATCAGAACG CCGACCCTCA 
TCTCGAGCTC AGGGGGCACG ACGCCCCATC GAAAGTCCTG CAACTGCACT CTGGGCTCAA 
AATGACACTG CCAGTCTAAG TGCTAACATA CTGTTAGCCC CTGCAGCACC GTCTCTGACT 
GCAACATTTG TTCCAGCACA TCCATCTGAA GTAATTCAGC CACCAGAAGA TGGGCCACTT 
GATCTGCATC CAGCTAGGCC AGATCCACAA CCTCCCTCTG AAAACCTTGA GATTCCTCCA 
GACTCTACAC CTCAGGCAAG TCTGGGATAT GCCTCCTTGC TTGTGACCTC ACCTCCAGAA 
GAGGCTCTGA ACCAGCCCGT CTTGATTGCG CCACCTTCCT CTAATTACAG TTTGATTTCA 
TCCCAAAATC TCTTACAACC ATCAATTCTG CCAAGTCCTC AAGAGACAGC AGCACCTGTT 
CACCCACATC CTCAATCTTC ATTGACCAAT CAAATGCCTC AGAATTCTCC TCCAACAAAT 
CAGCCACCAC TTCTTTTTTC TCAGACACCA GTAAACTTCA GTTCTTCCTC AGCTCAAGGT 
GTCTTGAATC TAACGCGAGA GGCCAACGAT GGTCAGGCAG CCCGACCAAA CCTCTCAAGG 
GCCCAGTCTC TTAAAGGTGA TAGCCAATTT CTTCCGCCTT CTGCTACTCA AGCTCCGCCT 
TCTTCAGCTG CCTCCACCAA TCACAACCAA CCTTCAAATT ATCAGCTGCT TGATTTTTCT 
ATGCATCAGC CACAGTCTAC AAATCAGGTC ACATCAGTCC CAGCTGTCTC TCAATCAACC 
CATCCTGCAG GCTTGGTGAA TAACACACCT GGCTTTTACC TGCAGGTCAC CAAAGATGCA 
CAGCAAGGGG GACAGTCTGA CACTGATTCT CTGTCCCATC AGCAGAACTC CAACCCCCCA 
TTACCCTCTA GCAACCAACT TGACAATAAT CAGACTCTGA ACTCCCATCC TCCTCCACCT 
CCAGCTCAAA ACCAGCCTCC TCCACAGGCC CCTCCAACAC ATCCTGGCGC CCCTCCTACT 
GGACCTGGAT CTGCCCAACC ACCTCCTTAC CCACCCCAAT CTGGAAACTC TACCACCCAA 
CCACCTCAAG AACCTCAAAG GCCTCCTTCA TCTCTAGGTA GTCAATCAGG TTATGGGGTT 
CCTCCTGCCA TGCCCCCTGG TCCAGGTTAC CCAGGGTACT ACCCAGGAGC CTACCCGGAG 
TACCAGGATG GAAGAGTACC ATACCCACCG CAGTATCCTG TGGATCCCAG AACTCAAAAT 
TATTATCAGG ATGATCCCTA CCGTAGAGGC GATCCTCGAT ACGGATGGTA TGATGGACAG 
AATCCTGGCT ATCGGGAGGC TGAACGCCAA CCAGAGAGGC CAAGCTCTAG AACCAGTCAA 
TACTCTGACA GGCCCAGCTC AAGACAGGGG TATCAAGAAG ACTTTTCAAG ATCCAGCCGT 
AGTGCCTATG ACGAATACTA TGCAAACTAC TATAAGAGAC AATACGATGC CTATGGAGAT 
AGGAGCAGGT GGTATGATCC CAATGCTGCT TATGATCCAC GCTACAAAGC ATACTATGAT 
CAAATGTATG GATGGTATTA TAACCAAGAT GGTTACAGAG GAGGACAGGC GTATTACAAC 
CAACAATATC AAAACAGGGG AAGGGAAGGG TATGACGATC CATGGCGATA TTACCCTGGT 
TATGACTCCA GCTTTGACGA TGACTCTCGG AGACGTGACC CATATGCTGA TGATTTTGAC 
CGGCGTTCGG TTCACAGTGA ACAATCAGCA CACAGTGTGC ATTCATCACA CAGTCGACGT 
AGTAGCTTCA GCTCACGGTC ACAACAGAGT CAGGTTTACA GGAGTCAACC AGACTTGGTC 
AGCGCAGCCT ACGATGCCAC TGGCACCACT CTTCCAGCAG ATTACACCTA CAGCCAGTAT 
CCTAACCCTT CAGATACTGT GAATTATGGT CAGGATCCTT ATAATTCTGC TGACAACACC 
TGGTCTGCAC CAGAACAGCC TCCTCCAAGA CCGTTGACCC CGGAGAAGTT TTCTATTCCT 
CATCGTTGTG CTCGTTTTGG TCCAGCTGGT CAGCTGATCC TTGTCCAACC CAACCTGCCC 
TCTGCTGGTC AGCCCACACT AGTAGAAATC CACAGTATGG AGACGCTCCT GCAGGATTCT 
CCAGAGCAGT CAGACATGCG TTCATTCCCA GGCCCGCTGG TCAAAGAGGA GACTCATAAG 
GTGGATGTCA TGAAGTTTGC TCAGAACAAA GCCCAAGAGT GTTTGCGAAA CGATGACCTC 
ATCGACAAAG ACTCCGCCTA CCTCATCTGG GAGTTCATTG TGCTGCTGTG TCGCCAGAAT 
GGGACCGTAG TGGGTACAGA TATTGCTGAC CTGCTGCTGA AGGAGCATCG CTCTGTGTGG 
TTGCCAGGAA AATCACCCAA TGAGGCCAAT CTCATTGACT TTAACAATGA GGCAATAGAG 
CATGCAGAAG AAGACATGGG GCAAATGTCC CTCCTATCAG ACACGTTCAT GGGTGTGCCT 
GAAAATATTG GCAAGGATAC GGAGCGCTTC AGAGAACTAC TGCTGTTTGG CCGCAAGAAG 
GACGCTCTGG AGTCTGCTAT GAAGAATGGT CTGTGGGGTC ACGCTCTGTT ACTGGCCAGT 
AAAATGGACA ACAGAACTCA CGCTCGCGTC ATGACCAGGT TTGCCAACAG CCTGCCTATT 
AATGACCCTC TTCAGACAGT TTATCAGCTC ATGTCAGGAC GTATGCCGGC TGCTGCTACT 
TGTTGTGGAG ATGAGAAATG GGGGGATTGG AGGCCACATT TAGCCATGGT GCTGTCCAAC 
CTCACTCACA CTCTGGATCT TGACACCCGC ACTATTTCAA CAATGGGTGA CACTTTAGCA 
TCTAAAGGTC TTGTAGACGC AGCCCATTTC TGTTACCTGA TGGCTCAGGT GGGTTTCGGT 
GTTTATACCA AGAAAAGCAC CAAGATGGTC CTCATCAGCT CCAATCACAG CTTGCCTTTT 
TTGAAGTTCT GCTCATCAGA GGCAATTCAG CGGACAGAAT CATACGAATA CGCTCAGTCT 
CTTGGCTCTC AGCCTCTTTC TCTGCCTAAC TTCCAGGTGT TCAAGTTCAT CTACGCATGT 
CGTCTGGCGG AGTCTGGTCT CTGCGCTCAA GCTTTTCATT ACTGTGAGGT CATTTCCCGC 
ACACTGTTGA GTCTGCCGGA GTATTACTCT CCTGTGTTCA TCAGTCAGCT CATACAGATG 
TCTCTGAGGT TAAGGTTTTT TGACCCTCAG CTGAAGGAAC GGCCGGAGCA GGAGTTGTTT 
ATAGAGCCTG ATTGGTTACT TCATCTCAGA CAACTCGATG GACAGATCAA GGAGGGTGTG 
ATCTCTTTGC GTACGGACCG CAGCACCCCA CAGCTTTACC CCTGCAGTAC ACCCAGTTCA 
GAAGACCAGT GCAGCCCGCC TGAACCCCCA GCTCTGACCC CAGACCCCCA CAACGCTCTC 
ATGACCTCTC TTGTGCCCCC AGCTGCTGTA CAACTCATGC CTCCAGCCCC TCCCACTATT 
CTTCAGGATG GGGCGGTTTC TCTTCAGCAA GTTCCGCCTC CAAGTGAAAG TGTTCCGTTC 
TACCCAGTGG CTCCAGGGCC GCCGCAGCCA AACTTTGTGG CTCCGTACCA ACCTGAAATG 
CAAGAGCAGT ACATGCCAAT GCCACCCCAA ATACCCCAAC ATATGCCCCC GCAAATGCCG 
ACATACAACC CTGCAGAAAT GCCTCCACAG ATGCCACAAG GAATGCCTGG TCAGATGCCT 
CCACCGATGC ACGGAGCCGA ACGGGGCTCA GCTCCTTCCT CCCCTCAGCA GTTCCCCCAG 
CCGTCTCCAA CGTTCGCTAA CCCTCCTCCA CCGGGGAAGG ACTTCTATGA TGAAATGGCA 
CGTTTGGGCC CAGGCAGAAG ATCAAGAACA ACTTCACAGT CATCAATGCA TATGGCACCG 
GGTCGTCGAT CTCGGACAAC ATCTGAATCG TCCAATCATT CTGGCCGGGA ACGCAGCAGC 
TCATTTGCCA ATCAGGGATC TCCACCCCCT CCACCAATCC CAGAGGCTCC ACCACAGGAT 
GTGCCAGAGA GAACCAAGAA AGACTCTCCT AAAAAACAGG GATCTGGTGG CGGCTTGCTG 
ACCTGGCTGT TTCCAAAGCG GAAGAATGAA GCACATCTTC CAGACGACAA AAACAAATCT 
ATTGTGTGGG ATGAAAAGAA GCAGAAATGG GTGAATCTGG ATGAGCCAGA GGAAGAGAGT 
AAACCTCCTC CTCCCCCACC CACCGGCTTC CCCAAAGCTC CAATGAGCGC TCCAGCTATG 
GTACCTCCAG GAGTGGCCGG CCCACCATCA GGGCCTCCTG TGAACATGTT CTCCAGAAGA 
GCAGGCACGA GGAGTCGTTA TGTGGATGTG CTGAATCCTG GTCGTGGAGG AGCTAAAGCA 
GTGGCTTCAG TGCCTCCTCC TGCTGATCTG TTCGCTCCTC TCGCACCCAT GGCCATGCCA 
GCCAACCTCT TCACACCAGC TGCAGCCCCA GATGAGCAGC AGCCGATGGA GGGAAGTGTT 
CCAGACACCA GTGGAGAAAA CACACTGCAG ACAAGTGCCA CAGTGCCACA GATGTTTAAT 
CCGAATGCTG CTTTGCCTCC CGGCCCGGAG GGAACCCAGT CTGGAGAGGC TCCTGCTCAA 
CAACCAGTGC AAGGAGCTCC GCCTGCAGGG GGTGTGACCT TTTATAACCC CTCCCAGTTT 
GCACAGTCTG TTCCCACAAA CCGTACTCGC CCTGGGCGCC TGGGACAGAG AGGATACCCA 
ACACCGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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sec16a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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