ANO2 cDNA ORF clone, Danio rerio(Zebrafish)

The following ANO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa09078 XM_005164485.4
Latest version!
Danio rerio anoctamin 2 (ano2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa44860 NM_001327858.1
Latest version!
Danio rerio anoctamin 2b (ano2b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa44861 XM_009299978.3
Latest version!
Danio rerio anoctamin 2 (ano2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa09078 XM_005164485.3 Danio rerio anoctamin 2 (ano2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa44861 XM_009299978.2 Danio rerio anoctamin 2 (ano2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa09078
    Clone ID Related Accession (Same CDS sequence) XM_005164485.3 , XM_005164485.4
    Accession Version XM_005164485.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product anoctamin-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGCAGA CGAGTGGCAA CAAGATCCAC GTGGCCAATA ACATGATGGA CATGGACCAG 
    GACCCTCGCA AGCGGCTCTA CTTTGCTGAC GGCCGAAGGA AAGTGGACTA TGTGCTGGTG
    TTCCACTACC GAAAGCGTTC CTCCATCAGG GGGTCTCCTA GTCAGCACAG ACTGTCCATC
    ATCTCCAATG GAAGTTATCA TTTAGGAGTG GAGGAAAAAG ACCCAGAAGG AGGGAAAGAG
    GAGCCTGCGG AGGTGGTGGT TGATGTTGGA CCACCGGACC CTGCCGAAGG GGAGAAAATC
    ATGATCCGGG AAGAGTTTGA GGGCAGTTTA AAGGACGCAG GGCTGGAAAT TGAACGTGAT
    AAAGAGAACA AGGCTCATGG TCATGCCTTT ATCCGGTTAC ACGCGCCTTG GCAGGTATTG
    AGCAGAGAGG CAGAGTTTCT CAAGATCAAG GTGCAGACCA AGAAGAGTTA CGAGCTGAGG
    GAAGAAAAAG GTCTGGCCGC CACTATGAAT GAAGTGTGGC GGAAGTTAAA TCAACCGTTC
    CAGCCCAAAG TACCCCATCA GGAGCAAGAG AACAGCCGCA CCAAGTTCCT CACCCACTGT
    TTCTCCCGGG ACAAGCTCCA CCTGTACAAC ATACAGTCAA AGGACACCTT CTTCGACAAT
    GCCACAAGGA GCCGAATAGT GTATGAAATC CTCAGACGCA CAGCCTGCAC ACGGACCTGC
    CAAACCATGG GTATCACCAC ACTCATAGCT AACGGGGTTT ATGATTCAGC ATTCCCATTG
    CACGATGGTG ACTTCCACTC CTCTGGACAA GCCGAGGGAA AAAACGAAAG ACAACTGTTA
    CATGACGAAT GGGCAAGATA TGGTGCTTTT TACAAGTATC AGCCGATCGA TCTCATCAGG
    AAGTACTTTG GCGAGAAGAT TGGTCTGTAC TTTGCCTGGC TTGGTGTGTA TACTCAGCTT
    CTGATCCCTG CATCTGTGGT GGGCATTATC GTCTTCTTCT ACGGCTGGGC CACTGTGGAA
    ACCAACGTAC CTAGCCAAGA GATGTGTGAC GAGACTCAGA ACTTCACTAT GTGTCCGCTC
    TGTGATCGGG TGTGTGACTA CTGGCAGCTG AGCACAGCGT GCGGTACGGC ACGTGCCAGC
    CATCTCTTTG ACAACCCGGC CACCGTGTTC TTCGCCATCT TCATGTCCCT CTGGGCTGCG
    ATGTTTCTGG AGCACTGGAA GCGCCGACAG ATCAGCCTGA ACTATAGCTG GGATCTGACA
    GGCATGGAGG AGGAGGAGGA GCATCCCAGA CCCAAATACG AAACCATCCT CCTTCAGAAG
    AGGCAGAGGA AGAAGAAAAA CAAGAAGAAC AAAAACGAGC CTGAGAGAGT TGTGGACGAG
    AGCGGGGAAC TGGGCAAGGA CAAATGGAGG CAGAGGTTAC TGTCAGCCAT GGCTGCTGGG
    AATCCGACTG GACATGAGAA GCTAACATGG AGGGACAGAC TGCCTGGATA TCTAATCAAC
    ATCTCCTCCA TTCTGTTCAT GTTTGGAGTG ACGTGCTCTG CAGTCTTCAG CGTCATCATT
    TACCGAATCA CCATCTCCGC CCTTATGGCC ATGAGCCCAG ACCCTGATGT TAAATCCAAC
    GTGAGGGTGA CCGTCACAGC CACTGCTGTC ATCATCAACC TGGTGGTCAT CCTCATTTTG
    GATGAAATCT ATGGCATGGT GGCTGCCTGG CTAACAGAAC TGGAGATCCC GAAAACTGAG
    ACCAACTTTG AAGAGCGTCT GATTCTGAAG GCTTTCGTCC TGAAGTTCAT GAATGCCTAT
    GCACCGATCT TTTACGTGGC CTTCTTCAAA GGGAGGTTTG CGGGCCGCCC TGGTGACTAT
    GTATATGTCT TTCATGACTA TCGAATGGAA GAATGTGCTC CTGGTGGCTG TCTAATTGAG
    CTCTGCATCC AGCTCAGCAT CATCATGTTG GGGAAGCAGC TTATTCAGAA CAACGTCTTC
    GAGATCGGCA TTCCAAAGCT GAAGAAGTTG TGCCGCACGC TAAAAGACAA AGATCGGGAG
    CCCAAGGAGA TTGTGGAGCA GCCGGGCCGC CGCCAACAGT GGCACATGGA CTACGACCTG
    GAGCCGTTTG ACGGACTCAC ACCTGAATAC ATGGAGATGA TCATACAGTT TGGTTTCGTG
    TCACTCTTTG TGGCCTCATT CCCGCTGGCT CCGCTATTCG CACTCCTAAA CAATGTCATC
    GAGATTCGCC TCGACGCCAA GAAGTTTGTC ACGGAGCTCC GCCGGCCAGA CGCCGTGAGG
    ACCAAAGACA TTGGCATCTG GTATAACATC TTGAGCGGTT TGGGAAAGTT CTCAGTCATC
    ATCAATGCCT TTGTCATCTC CTTCACATCT GATTTCATCC CTCGCCTGGT CTACCAGTAC
    ATGTACAGCG AGACAGGAAC CATGCACGGC TTCATCAACC ACACGCTATC TTACTTTAAC
    GTCAGCAACT TCAAACCAGG AACCATGCCT TCAACATCCG ACATTACAGT CTGCAGGTAC
    AAGGACTACA GAGAGCCTCC ATGGTCGAAT GAGCCTTACC AGTTCTCCAA ACAGTACTGG
    TCCGTGCTAG CGGCTCGGTT AGGTTTCGTC ATTTTATTCC AGAACCTGGT GATAATCATG
    AGCATGCTGG TGGCCTGGCT GATCCCGGAC ATGCCCAAAA ACATCAGTGA GCAGTTAAAG
    AAGGACAAGA CTCTTCTAGT GGACGTCTTC CTGAACGAAG AAAAGGAGAA GCTGCAGCTG
    ATCCAGAGCC TGTTCTCCAA AGATCTGACC AACAAGCAGC AGGAAGAGCA CCAGCAGCAG
    GTTTCGGATA AAACCTCGCT GCCTCTCCAG CAAAGCCAGC CTGGCTTTCT CCCTCCGTCC
    TCATCCCCTG CCTCTACAGC TCCTCGAACC CGAGCACGAG CCGCCAGCTT CAGTCAGTTC
    ACCCGGCAGA TGTCTATGTC TCCCCGCAGG GACGTCACCC AGCACACGGC GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164542.1
    CDS466..3462
    Translation

    Target ORF information:

    RefSeq Version XM_005164485.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio anoctamin 2 (ano2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164485.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGCAGA CGAGTGGCAA CAAGATCCAC GTGGCCAATA ACATGATGGA CATGGACCAG 
    GACCCTCGCA AGCGGCTCTA CTTTGCTGAC GGCCGAAGGA AAGTGGACTA TGTGCTGGTG
    TTCCACTACC GAAAGCGTTC CTCCATCAGG GGGTCTCCTA GTCAGCACAG ACTGTCCATC
    ATCTCCAATG GAAGTTATCA TTTAGGAGTG GAGGAAAAAG ACCCAGAAGG AGGGAAAGAG
    GAGCCTGCGG AGGTGGTGGT TGATGTTGGA CCACCGGACC CTGCCGAAGG GGAGAAAATC
    ATGATCCGGG AAGAGTTTGA GGGCAGTTTA AAGGACGCAG GGCTGGAAAT TGAACGTGAT
    AAAGAGAACA AGGCTCATGG TCATGCCTTT ATCCGGTTAC ACGCGCCTTG GCAGGTATTG
    AGCAGAGAGG CAGAGTTTCT CAAGATCAAG GTGCAGACCA AGAAGAGTTA CGAGCTGAGG
    GAAGAAAAAG GTCTGGCCGC CACTATGAAT GAAGTGTGGC GGAAGTTAAA TCAACCGTTC
    CAGCCCAAAG TACCCCATCA GGAGCAAGAG AACAGCCGCA CCAAGTTCCT CACCCACTGT
    TTCTCCCGGG ACAAGCTCCA CCTGTACAAC ATACAGTCAA AGGACACCTT CTTCGACAAT
    GCCACAAGGA GCCGAATAGT GTATGAAATC CTCAGACGCA CAGCCTGCAC ACGGACCTGC
    CAAACCATGG GTATCACCAC ACTCATAGCT AACGGGGTTT ATGATTCAGC ATTCCCATTG
    CACGATGGTG ACTTCCACTC CTCTGGACAA GCCGAGGGAA AAAACGAAAG ACAACTGTTA
    CATGACGAAT GGGCAAGATA TGGTGCTTTT TACAAGTATC AGCCGATCGA TCTCATCAGG
    AAGTACTTTG GCGAGAAGAT TGGTCTGTAC TTTGCCTGGC TTGGTGTGTA TACTCAGCTT
    CTGATCCCTG CATCTGTGGT GGGCATTATC GTCTTCTTCT ACGGCTGGGC CACTGTGGAA
    ACCAACGTAC CTAGCCAAGA GATGTGTGAC GAGACTCAGA ACTTCACTAT GTGTCCGCTC
    TGTGATCGGG TGTGTGACTA CTGGCAGCTG AGCACAGCGT GCGGTACGGC ACGTGCCAGC
    CATCTCTTTG ACAACCCGGC CACCGTGTTC TTCGCCATCT TCATGTCCCT CTGGGCTGCG
    ATGTTTCTGG AGCACTGGAA GCGCCGACAG ATCAGCCTGA ACTATAGCTG GGATCTGACA
    GGCATGGAGG AGGAGGAGGA GCATCCCAGA CCCAAATACG AAACCATCCT CCTTCAGAAG
    AGGCAGAGGA AGAAGAAAAA CAAGAAGAAC AAAAACGAGC CTGAGAGAGT TGTGGACGAG
    AGCGGGGAAC TGGGCAAGGA CAAATGGAGG CAGAGGTTAC TGTCAGCCAT GGCTGCTGGG
    AATCCGACTG GACATGAGAA GCTAACATGG AGGGACAGAC TGCCTGGATA TCTAATCAAC
    ATCTCCTCCA TTCTGTTCAT GTTTGGAGTG ACGTGCTCTG CAGTCTTCAG CGTCATCATT
    TACCGAATCA CCATCTCCGC CCTTATGGCC ATGAGCCCAG ACCCTGATGT TAAATCCAAC
    GTGAGGGTGA CCGTCACAGC CACTGCTGTC ATCATCAACC TGGTGGTCAT CCTCATTTTG
    GATGAAATCT ATGGCATGGT GGCTGCCTGG CTAACAGAAC TGGAGATCCC GAAAACTGAG
    ACCAACTTTG AAGAGCGTCT GATTCTGAAG GCTTTCGTCC TGAAGTTCAT GAATGCCTAT
    GCACCGATCT TTTACGTGGC CTTCTTCAAA GGGAGGTTTG CGGGCCGCCC TGGTGACTAT
    GTATATGTCT TTCATGACTA TCGAATGGAA GAATGTGCTC CTGGTGGCTG TCTAATTGAG
    CTCTGCATCC AGCTCAGCAT CATCATGTTG GGGAAGCAGC TTATTCAGAA CAACGTCTTC
    GAGATCGGCA TTCCAAAGCT GAAGAAGTTG TGCCGCACGC TAAAAGACAA AGATCGGGAG
    CCCAAGGAGA TTGTGGAGCA GCCGGGCCGC CGCCAACAGT GGCACATGGA CTACGACCTG
    GAGCCGTTTG ACGGACTCAC ACCTGAATAC ATGGAGATGA TCATACAGTT TGGTTTCGTG
    TCACTCTTTG TGGCCTCATT CCCGCTGGCT CCGCTATTCG CACTCCTAAA CAATGTCATC
    GAGATTCGCC TCGACGCCAA GAAGTTTGTC ACGGAGCTCC GCCGGCCAGA CGCCGTGAGG
    ACCAAAGACA TTGGCATCTG GTATAACATC TTGAGCGGTT TGGGAAAGTT CTCAGTCATC
    ATCAATGCCT TTGTCATCTC CTTCACATCT GATTTCATCC CTCGCCTGGT CTACCAGTAC
    ATGTACAGCG AGACAGGAAC CATGCACGGC TTCATCAACC ACACGCTATC TTACTTTAAC
    GTCAGCAACT TCAAACCAGG AACCATGCCT TCAACATCCG ACATTACAGT CTGCAGGTAC
    AAGGACTACA GAGAGCCTCC ATGGTCGAAT GAGCCTTACC AGTTCTCCAA ACAGTACTGG
    TCCGTGCTAG CGGCTCGGTT AGGTTTCGTC ATTTTATTCC AGAACCTGGT GATAATCATG
    AGCATGCTGG TGGCCTGGCT GATCCCGGAC ATGCCCAAAA ACATCAGTGA GCAGTTAAAG
    AAGGACAAGA CTCTTCTAGT GGACGTCTTC CTGAACGAAG AAAAGGAGAA GCTGCAGCTG
    ATCCAGAGCC TGTTCTCCAA AGATCTGACC AACAAGCAGC AGGAAGAGCA CCAGCAGCAG
    GTTTCGGATA AAACCTCGCT GCCTCTCCAG CAAAGCCAGC CTGGCTTTCT CCCTCCGTCC
    TCATCCCCTG CCTCTACAGC TCCTCGAACC CGAGCACGAG CCGCCAGCTT CAGTCAGTTC
    ACCCGGCAGA TGTCTATGTC TCCCCGCAGG GACGTCACCC AGCACACGGC GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09078
    Clone ID Related Accession (Same CDS sequence) XM_005164485.3 , XM_005164485.4
    Accession Version XM_005164485.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164485.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGCAGA CGAGTGGCAA CAAGATCCAC GTGGCCAATA ACATGATGGA CATGGACCAG 
    GACCCTCGCA AGCGGCTCTA CTTTGCTGAC GGCCGAAGGA AAGTGGACTA TGTGCTGGTG
    TTCCACTACC GAAAGCGTTC CTCCATCAGG GGGTCTCCTA GTCAGCACAG ACTGTCCATC
    ATCTCCAATG GAAGTTATCA TTTAGGAGTG GAGGAAAAAG ACCCAGAAGG AGGGAAAGAG
    GAGCCTGCGG AGGTGGTGGT TGATGTTGGA CCACCGGACC CTGCCGAAGG GGAGAAAATC
    ATGATCCGGG AAGAGTTTGA GGGCAGTTTA AAGGACGCAG GGCTGGAAAT TGAACGTGAT
    AAAGAGAACA AGGCTCATGG TCATGCCTTT ATCCGGTTAC ACGCGCCTTG GCAGGTATTG
    AGCAGAGAGG CAGAGTTTCT CAAGATCAAG GTGCAGACCA AGAAGAGTTA CGAGCTGAGG
    GAAGAAAAAG GTCTGGCCGC CACTATGAAT GAAGTGTGGC GGAAGTTAAA TCAACCGTTC
    CAGCCCAAAG TACCCCATCA GGAGCAAGAG AACAGCCGCA CCAAGTTCCT CACCCACTGT
    TTCTCCCGGG ACAAGCTCCA CCTGTACAAC ATACAGTCAA AGGACACCTT CTTCGACAAT
    GCCACAAGGA GCCGAATAGT GTATGAAATC CTCAGACGCA CAGCCTGCAC ACGGACCTGC
    CAAACCATGG GTATCACCAC ACTCATAGCT AACGGGGTTT ATGATTCAGC ATTCCCATTG
    CACGATGGTG ACTTCCACTC CTCTGGACAA GCCGAGGGAA AAAACGAAAG ACAACTGTTA
    CATGACGAAT GGGCAAGATA TGGTGCTTTT TACAAGTATC AGCCGATCGA TCTCATCAGG
    AAGTACTTTG GCGAGAAGAT TGGTCTGTAC TTTGCCTGGC TTGGTGTGTA TACTCAGCTT
    CTGATCCCTG CATCTGTGGT GGGCATTATC GTCTTCTTCT ACGGCTGGGC CACTGTGGAA
    ACCAACGTAC CTAGCCAAGA GATGTGTGAC GAGACTCAGA ACTTCACTAT GTGTCCGCTC
    TGTGATCGGG TGTGTGACTA CTGGCAGCTG AGCACAGCGT GCGGTACGGC ACGTGCCAGC
    CATCTCTTTG ACAACCCGGC CACCGTGTTC TTCGCCATCT TCATGTCCCT CTGGGCTGCG
    ATGTTTCTGG AGCACTGGAA GCGCCGACAG ATCAGCCTGA ACTATAGCTG GGATCTGACA
    GGCATGGAGG AGGAGGAGGA GCATCCCAGA CCCAAATACG AAACCATCCT CCTTCAGAAG
    AGGCAGAGGA AGAAGAAAAA CAAGAAGAAC AAAAACGAGC CTGAGAGAGT TGTGGACGAG
    AGCGGGGAAC TGGGCAAGGA CAAATGGAGG CAGAGGTTAC TGTCAGCCAT GGCTGCTGGG
    AATCCGACTG GACATGAGAA GCTAACATGG AGGGACAGAC TGCCTGGATA TCTAATCAAC
    ATCTCCTCCA TTCTGTTCAT GTTTGGAGTG ACGTGCTCTG CAGTCTTCAG CGTCATCATT
    TACCGAATCA CCATCTCCGC CCTTATGGCC ATGAGCCCAG ACCCTGATGT TAAATCCAAC
    GTGAGGGTGA CCGTCACAGC CACTGCTGTC ATCATCAACC TGGTGGTCAT CCTCATTTTG
    GATGAAATCT ATGGCATGGT GGCTGCCTGG CTAACAGAAC TGGAGATCCC GAAAACTGAG
    ACCAACTTTG AAGAGCGTCT GATTCTGAAG GCTTTCGTCC TGAAGTTCAT GAATGCCTAT
    GCACCGATCT TTTACGTGGC CTTCTTCAAA GGGAGGTTTG CGGGCCGCCC TGGTGACTAT
    GTATATGTCT TTCATGACTA TCGAATGGAA GAATGTGCTC CTGGTGGCTG TCTAATTGAG
    CTCTGCATCC AGCTCAGCAT CATCATGTTG GGGAAGCAGC TTATTCAGAA CAACGTCTTC
    GAGATCGGCA TTCCAAAGCT GAAGAAGTTG TGCCGCACGC TAAAAGACAA AGATCGGGAG
    CCCAAGGAGA TTGTGGAGCA GCCGGGCCGC CGCCAACAGT GGCACATGGA CTACGACCTG
    GAGCCGTTTG ACGGACTCAC ACCTGAATAC ATGGAGATGA TCATACAGTT TGGTTTCGTG
    TCACTCTTTG TGGCCTCATT CCCGCTGGCT CCGCTATTCG CACTCCTAAA CAATGTCATC
    GAGATTCGCC TCGACGCCAA GAAGTTTGTC ACGGAGCTCC GCCGGCCAGA CGCCGTGAGG
    ACCAAAGACA TTGGCATCTG GTATAACATC TTGAGCGGTT TGGGAAAGTT CTCAGTCATC
    ATCAATGCCT TTGTCATCTC CTTCACATCT GATTTCATCC CTCGCCTGGT CTACCAGTAC
    ATGTACAGCG AGACAGGAAC CATGCACGGC TTCATCAACC ACACGCTATC TTACTTTAAC
    GTCAGCAACT TCAAACCAGG AACCATGCCT TCAACATCCG ACATTACAGT CTGCAGGTAC
    AAGGACTACA GAGAGCCTCC ATGGTCGAAT GAGCCTTACC AGTTCTCCAA ACAGTACTGG
    TCCGTGCTAG CGGCTCGGTT AGGTTTCGTC ATTTTATTCC AGAACCTGGT GATAATCATG
    AGCATGCTGG TGGCCTGGCT GATCCCGGAC ATGCCCAAAA ACATCAGTGA GCAGTTAAAG
    AAGGACAAGA CTCTTCTAGT GGACGTCTTC CTGAACGAAG AAAAGGAGAA GCTGCAGCTG
    ATCCAGAGCC TGTTCTCCAA AGATCTGACC AACAAGCAGC AGGAAGAGCA CCAGCAGCAG
    GTTTCGGATA AAACCTCGCT GCCTCTCCAG CAAAGCCAGC CTGGCTTTCT CCCTCCGTCC
    TCATCCCCTG CCTCTACAGC TCCTCGAACC CGAGCACGAG CCGCCAGCTT CAGTCAGTTC
    ACCCGGCAGA TGTCTATGTC TCCCCGCAGG GACGTCACCC AGCACACGGC GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164542.1
    CDS466..3462
    Translation

    Target ORF information:

    RefSeq Version XM_005164485.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 2 (ano2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164485.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGCAGA CGAGTGGCAA CAAGATCCAC GTGGCCAATA ACATGATGGA CATGGACCAG 
    GACCCTCGCA AGCGGCTCTA CTTTGCTGAC GGCCGAAGGA AAGTGGACTA TGTGCTGGTG
    TTCCACTACC GAAAGCGTTC CTCCATCAGG GGGTCTCCTA GTCAGCACAG ACTGTCCATC
    ATCTCCAATG GAAGTTATCA TTTAGGAGTG GAGGAAAAAG ACCCAGAAGG AGGGAAAGAG
    GAGCCTGCGG AGGTGGTGGT TGATGTTGGA CCACCGGACC CTGCCGAAGG GGAGAAAATC
    ATGATCCGGG AAGAGTTTGA GGGCAGTTTA AAGGACGCAG GGCTGGAAAT TGAACGTGAT
    AAAGAGAACA AGGCTCATGG TCATGCCTTT ATCCGGTTAC ACGCGCCTTG GCAGGTATTG
    AGCAGAGAGG CAGAGTTTCT CAAGATCAAG GTGCAGACCA AGAAGAGTTA CGAGCTGAGG
    GAAGAAAAAG GTCTGGCCGC CACTATGAAT GAAGTGTGGC GGAAGTTAAA TCAACCGTTC
    CAGCCCAAAG TACCCCATCA GGAGCAAGAG AACAGCCGCA CCAAGTTCCT CACCCACTGT
    TTCTCCCGGG ACAAGCTCCA CCTGTACAAC ATACAGTCAA AGGACACCTT CTTCGACAAT
    GCCACAAGGA GCCGAATAGT GTATGAAATC CTCAGACGCA CAGCCTGCAC ACGGACCTGC
    CAAACCATGG GTATCACCAC ACTCATAGCT AACGGGGTTT ATGATTCAGC ATTCCCATTG
    CACGATGGTG ACTTCCACTC CTCTGGACAA GCCGAGGGAA AAAACGAAAG ACAACTGTTA
    CATGACGAAT GGGCAAGATA TGGTGCTTTT TACAAGTATC AGCCGATCGA TCTCATCAGG
    AAGTACTTTG GCGAGAAGAT TGGTCTGTAC TTTGCCTGGC TTGGTGTGTA TACTCAGCTT
    CTGATCCCTG CATCTGTGGT GGGCATTATC GTCTTCTTCT ACGGCTGGGC CACTGTGGAA
    ACCAACGTAC CTAGCCAAGA GATGTGTGAC GAGACTCAGA ACTTCACTAT GTGTCCGCTC
    TGTGATCGGG TGTGTGACTA CTGGCAGCTG AGCACAGCGT GCGGTACGGC ACGTGCCAGC
    CATCTCTTTG ACAACCCGGC CACCGTGTTC TTCGCCATCT TCATGTCCCT CTGGGCTGCG
    ATGTTTCTGG AGCACTGGAA GCGCCGACAG ATCAGCCTGA ACTATAGCTG GGATCTGACA
    GGCATGGAGG AGGAGGAGGA GCATCCCAGA CCCAAATACG AAACCATCCT CCTTCAGAAG
    AGGCAGAGGA AGAAGAAAAA CAAGAAGAAC AAAAACGAGC CTGAGAGAGT TGTGGACGAG
    AGCGGGGAAC TGGGCAAGGA CAAATGGAGG CAGAGGTTAC TGTCAGCCAT GGCTGCTGGG
    AATCCGACTG GACATGAGAA GCTAACATGG AGGGACAGAC TGCCTGGATA TCTAATCAAC
    ATCTCCTCCA TTCTGTTCAT GTTTGGAGTG ACGTGCTCTG CAGTCTTCAG CGTCATCATT
    TACCGAATCA CCATCTCCGC CCTTATGGCC ATGAGCCCAG ACCCTGATGT TAAATCCAAC
    GTGAGGGTGA CCGTCACAGC CACTGCTGTC ATCATCAACC TGGTGGTCAT CCTCATTTTG
    GATGAAATCT ATGGCATGGT GGCTGCCTGG CTAACAGAAC TGGAGATCCC GAAAACTGAG
    ACCAACTTTG AAGAGCGTCT GATTCTGAAG GCTTTCGTCC TGAAGTTCAT GAATGCCTAT
    GCACCGATCT TTTACGTGGC CTTCTTCAAA GGGAGGTTTG CGGGCCGCCC TGGTGACTAT
    GTATATGTCT TTCATGACTA TCGAATGGAA GAATGTGCTC CTGGTGGCTG TCTAATTGAG
    CTCTGCATCC AGCTCAGCAT CATCATGTTG GGGAAGCAGC TTATTCAGAA CAACGTCTTC
    GAGATCGGCA TTCCAAAGCT GAAGAAGTTG TGCCGCACGC TAAAAGACAA AGATCGGGAG
    CCCAAGGAGA TTGTGGAGCA GCCGGGCCGC CGCCAACAGT GGCACATGGA CTACGACCTG
    GAGCCGTTTG ACGGACTCAC ACCTGAATAC ATGGAGATGA TCATACAGTT TGGTTTCGTG
    TCACTCTTTG TGGCCTCATT CCCGCTGGCT CCGCTATTCG CACTCCTAAA CAATGTCATC
    GAGATTCGCC TCGACGCCAA GAAGTTTGTC ACGGAGCTCC GCCGGCCAGA CGCCGTGAGG
    ACCAAAGACA TTGGCATCTG GTATAACATC TTGAGCGGTT TGGGAAAGTT CTCAGTCATC
    ATCAATGCCT TTGTCATCTC CTTCACATCT GATTTCATCC CTCGCCTGGT CTACCAGTAC
    ATGTACAGCG AGACAGGAAC CATGCACGGC TTCATCAACC ACACGCTATC TTACTTTAAC
    GTCAGCAACT TCAAACCAGG AACCATGCCT TCAACATCCG ACATTACAGT CTGCAGGTAC
    AAGGACTACA GAGAGCCTCC ATGGTCGAAT GAGCCTTACC AGTTCTCCAA ACAGTACTGG
    TCCGTGCTAG CGGCTCGGTT AGGTTTCGTC ATTTTATTCC AGAACCTGGT GATAATCATG
    AGCATGCTGG TGGCCTGGCT GATCCCGGAC ATGCCCAAAA ACATCAGTGA GCAGTTAAAG
    AAGGACAAGA CTCTTCTAGT GGACGTCTTC CTGAACGAAG AAAAGGAGAA GCTGCAGCTG
    ATCCAGAGCC TGTTCTCCAA AGATCTGACC AACAAGCAGC AGGAAGAGCA CCAGCAGCAG
    GTTTCGGATA AAACCTCGCT GCCTCTCCAG CAAAGCCAGC CTGGCTTTCT CCCTCCGTCC
    TCATCCCCTG CCTCTACAGC TCCTCGAACC CGAGCACGAG CCGCCAGCTT CAGTCAGTTC
    ACCCGGCAGA TGTCTATGTC TCCCCGCAGG GACGTCACCC AGCACACGGC GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44860
    Clone ID Related Accession (Same CDS sequence) NM_001327858.1
    Accession Version NM_001327858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-07
    Organism Danio rerio(zebrafish)
    Product anoctamin-2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR293509.12 and CU468826.10. On May 27, 2016 this sequence version replaced XM_009299977.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA4476749, SAMEA4476775 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCGTCA ACAGATCCCG CTCGTATGTT GGGATGGATG ATCTGCATGT TCTGAATGGA 
    GTCAGCACTG CTTTGAGCGA GTCCTCATCA GTTTGCAGTG ATTTAAGGGA TATTTCTGAG
    ATTGAAGTCA TGAACTCCAT CAGTTGCACC TTTGATGACT CGGTTGACTT CATTGAGGAG
    TCTTTGCACC ACAGTGACAA CATGTTGCAG ACGAGTGGCA ACAAGATCCA CGTGGCCAAT
    AACATGATGG ACATGGACCA GGACCCTCGC AAGCGGCTCT ACTTTGCTGA CGGCCGAAGG
    AAAGTGGACT ATGTGCTGGT GTTCCACTAC CGAAAGCGTT CCTCCATCAG GGGGTCTCCT
    AGTCAGCACA GACTGTCCAT CATCTCCAAT GGAAGTTATC ATTTAGGAGT GGAGGAAAAA
    GACCCAGAAG GAGGGAAAGA GGAGCCTGCG GAGGTGGTGG TTGATGTTGG ACCACCGGAC
    CCTGCCGAAG GGGAGAAAAT CATGATCCGG GAAGAGTTTG AGGGCAGTTT AAAGGACGCA
    GGGCTGGAAA TTGAACGTGA TAAAGAGAAC AAGGCTCATG GTCATGCCTT TATCCGGTTA
    CACGCGCCTT GGCAGGTATT GAGCAGAGAG GCAGAGTTTC TCAAGATCAA GGTGCAGACC
    AAGAAGAGTT ACGAGCTGAG GGAAGAAAAA GGTCTGGCCG CCACTATGAA TGAAGTGTGG
    CGGAAGTTAA ATCAACCGTT CCAGCCCAAA GTACCCCATC AGGAGCAAGA GAACAGCCGC
    ACCAAGTTCC TCACCCACTG TTTCTCCCGG GACAAGCTCC ACCTGTACAA CATACAGTCA
    AAGGACACCT TCTTCGACAA TGCCACAAGG AGCCGAATAG TGTATGAAAT CCTCAGACGC
    ACAGCCTGCA CACGGACCTG CCAAACCATG GGTATCACCA CACTCATAGC TAACGGGGTT
    TATGATTCAG CATTCCCATT GCACGATGGT GACTTCCACT CCTCTGGACA AGCCGAGGGA
    AAAAACGAAA GACAACTGTT ACATGACGAA TGGGCAAGAT ATGGTGCTTT TTACAAGTAT
    CAGCCGATCG ATCTCATCAG GAAGTACTTT GGCGAGAAGA TTGGTCTGTA CTTTGCCTGG
    CTTGGTGTGT ATACTCAGCT TCTGATCCCT GCATCTGTGG TGGGCATTAT CGTCTTCTTC
    TACGGCTGGG CCACTGTGGA AACCAACGTA CCTAGCCAAG AGATGTGTGA CGAGACTCAG
    AACTTCACTA TGTGTCCGCT CTGTGATCGG GTGTGTGACT ACTGGCAGCT GAGCACAGCG
    TGCGGTACGG CACGTGCCAG CCATCTCTTT GACAACCCGG CCACCGTGTT CTTCGCCATC
    TTCATGTCCC TCTGGGCTGC GATGTTTCTG GAGCACTGGA AGCGCCGACA GATCAGCCTG
    AACTATAGCT GGGATCTGAC AGGCATGGAG GAGGAGGAGG AGCATCCCAG ACCCAAATAC
    GAAACCATCC TCCTTCAGAA GAGGCAGAGG AAGAAGAAAA ACAAGAAGAA CAAAAACGAG
    CCTGAGAGAG TTGTGGACGA GAGCGGGGAA CTGGGCAAGG ACAAATGGAG GCAGAGGTTA
    CTGTCAGCCA TGGCTGCTGG GAATCCGACT GGACATGAGA AGCTAACATG GAGGGACAGA
    CTGCCTGGAT ATCTAATCAA CATCTCCTCC ATTCTGTTCA TGTTTGGAGT GACGTGCTCT
    GCAGTCTTCA GCGTCATCAT TTACCGAATC ACCATCTCCG CCCTTATGGC CATGAGCCCA
    GACCCTGATG TTAAATCCAA CGTGAGGGTG ACCGTCACAG CCACTGCTGT CATCATCAAC
    CTGGTGGTCA TCCTCATTTT GGATGAAATC TATGGCATGG TGGCTGCCTG GCTAACAGAA
    CTGGAGATCC CGAAAACTGA GACCAACTTT GAAGAGCGTC TGATTCTGAA GGCTTTCGTC
    CTGAAGTTCA TGAATGCCTA TGCACCGATC TTTTACGTGG CCTTCTTCAA AGGGAGGTTT
    GCGGGCCGCC CTGGTGACTA TGTATATGTC TTTCATGACT ATCGAATGGA AGAATGTGCT
    CCTGGTGGCT GTCTAATTGA GCTCTGCATC CAGCTCAGCA TCATCATGTT GGGGAAGCAG
    CTTATTCAGA ACAACGTCTT CGAGATCGGC ATTCCAAAGC TGAAGAAGTT GTGCCGCACG
    CTAAAAGACA AAGATCGGGA GCCCAAGGAG ATTGTGGAGC AGCCGGGCCG CCGCCAACAG
    TGGCACATGG ACTACGACCT GGAGCCGTTT GACGGACTCA CACCTGAATA CATGGAGATG
    ATCATACAGT TTGGTTTCGT GTCACTCTTT GTGGCCTCAT TCCCGCTGGC TCCGCTATTC
    GCACTCCTAA ACAATGTCAT CGAGATTCGC CTCGACGCCA AGAAGTTTGT CACGGAGCTC
    CGCCGGCCAG ACGCCGTGAG GACCAAAGAC ATTGGCATCT GGTATAACAT CTTGAGCGGT
    TTGGGAAAGT TCTCAGTCAT CATCAATGCC TTTGTCATCT CCTTCACATC TGATTTCATC
    CCTCGCCTGG TCTACCAGTA CATGTACAGC GAGACAGGAA CCATGCACGG CTTCATCAAC
    CACACGCTAT CTTACTTTAA CGTCAGCAAC TTCAAACCAG GAACCATGCC TTCAACATCC
    GACATTACAG TCTGCAGGTA CAAGGACTAC AGAGAGCCTC CATGGTCGAA TGAGCCTTAC
    CAGTTCTCCA AACAGTACTG GTCCGTGCTA GCGGCTCGGT TAGGTTTCGT CATTTTATTC
    CAGAACCTGG TGATAATCAT GAGCATGCTG GTGGCCTGGC TGATCCCGGA CATGCCCAAA
    AACATCAGTG AGCAGTTAAA GAAGGACAAG ACTCTTCTAG TGGACGTCTT CCTGAACGAA
    GAAAAGGAGA AGCTGCAGCT GATCCAGAGC CTGTTCTCCA AAGATCTGAC CAACAAGCAG
    CAGGAAGAGC ACCAGCAGCA GGTTTCGGAT AAAACCTCGC TGCCTCTCCA GCAAAGCCAG
    CCTGGCTTTC TCCCTCCGTC CTCATCCCCT GCCTCTACAG CTCCTCGAAC CCGAGCACGA
    GCCGCCAGCT TCAGTCAGTT CACCCGGCAG ATGTCTATGT CTCCCCGCAG GGACGTCACC
    CAGCACACGG CGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001314787.1
    CDS48..3245
    Translation

    Target ORF information:

    RefSeq Version NM_001327858.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 2b (ano2b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001327858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCGTCA ACAGATCCCG CTCGTATGTT GGGATGGATG ATCTGCATGT TCTGAATGGA 
    GTCAGCACTG CTTTGAGCGA GTCCTCATCA GTTTGCAGTG ATTTAAGGGA TATTTCTGAG
    ATTGAAGTCA TGAACTCCAT CAGTTGCACC TTTGATGACT CGGTTGACTT CATTGAGGAG
    TCTTTGCACC ACAGTGACAA CATGTTGCAG ACGAGTGGCA ACAAGATCCA CGTGGCCAAT
    AACATGATGG ACATGGACCA GGACCCTCGC AAGCGGCTCT ACTTTGCTGA CGGCCGAAGG
    AAAGTGGACT ATGTGCTGGT GTTCCACTAC CGAAAGCGTT CCTCCATCAG GGGGTCTCCT
    AGTCAGCACA GACTGTCCAT CATCTCCAAT GGAAGTTATC ATTTAGGAGT GGAGGAAAAA
    GACCCAGAAG GAGGGAAAGA GGAGCCTGCG GAGGTGGTGG TTGATGTTGG ACCACCGGAC
    CCTGCCGAAG GGGAGAAAAT CATGATCCGG GAAGAGTTTG AGGGCAGTTT AAAGGACGCA
    GGGCTGGAAA TTGAACGTGA TAAAGAGAAC AAGGCTCATG GTCATGCCTT TATCCGGTTA
    CACGCGCCTT GGCAGGTATT GAGCAGAGAG GCAGAGTTTC TCAAGATCAA GGTGCAGACC
    AAGAAGAGTT ACGAGCTGAG GGAAGAAAAA GGTCTGGCCG CCACTATGAA TGAAGTGTGG
    CGGAAGTTAA ATCAACCGTT CCAGCCCAAA GTACCCCATC AGGAGCAAGA GAACAGCCGC
    ACCAAGTTCC TCACCCACTG TTTCTCCCGG GACAAGCTCC ACCTGTACAA CATACAGTCA
    AAGGACACCT TCTTCGACAA TGCCACAAGG AGCCGAATAG TGTATGAAAT CCTCAGACGC
    ACAGCCTGCA CACGGACCTG CCAAACCATG GGTATCACCA CACTCATAGC TAACGGGGTT
    TATGATTCAG CATTCCCATT GCACGATGGT GACTTCCACT CCTCTGGACA AGCCGAGGGA
    AAAAACGAAA GACAACTGTT ACATGACGAA TGGGCAAGAT ATGGTGCTTT TTACAAGTAT
    CAGCCGATCG ATCTCATCAG GAAGTACTTT GGCGAGAAGA TTGGTCTGTA CTTTGCCTGG
    CTTGGTGTGT ATACTCAGCT TCTGATCCCT GCATCTGTGG TGGGCATTAT CGTCTTCTTC
    TACGGCTGGG CCACTGTGGA AACCAACGTA CCTAGCCAAG AGATGTGTGA CGAGACTCAG
    AACTTCACTA TGTGTCCGCT CTGTGATCGG GTGTGTGACT ACTGGCAGCT GAGCACAGCG
    TGCGGTACGG CACGTGCCAG CCATCTCTTT GACAACCCGG CCACCGTGTT CTTCGCCATC
    TTCATGTCCC TCTGGGCTGC GATGTTTCTG GAGCACTGGA AGCGCCGACA GATCAGCCTG
    AACTATAGCT GGGATCTGAC AGGCATGGAG GAGGAGGAGG AGCATCCCAG ACCCAAATAC
    GAAACCATCC TCCTTCAGAA GAGGCAGAGG AAGAAGAAAA ACAAGAAGAA CAAAAACGAG
    CCTGAGAGAG TTGTGGACGA GAGCGGGGAA CTGGGCAAGG ACAAATGGAG GCAGAGGTTA
    CTGTCAGCCA TGGCTGCTGG GAATCCGACT GGACATGAGA AGCTAACATG GAGGGACAGA
    CTGCCTGGAT ATCTAATCAA CATCTCCTCC ATTCTGTTCA TGTTTGGAGT GACGTGCTCT
    GCAGTCTTCA GCGTCATCAT TTACCGAATC ACCATCTCCG CCCTTATGGC CATGAGCCCA
    GACCCTGATG TTAAATCCAA CGTGAGGGTG ACCGTCACAG CCACTGCTGT CATCATCAAC
    CTGGTGGTCA TCCTCATTTT GGATGAAATC TATGGCATGG TGGCTGCCTG GCTAACAGAA
    CTGGAGATCC CGAAAACTGA GACCAACTTT GAAGAGCGTC TGATTCTGAA GGCTTTCGTC
    CTGAAGTTCA TGAATGCCTA TGCACCGATC TTTTACGTGG CCTTCTTCAA AGGGAGGTTT
    GCGGGCCGCC CTGGTGACTA TGTATATGTC TTTCATGACT ATCGAATGGA AGAATGTGCT
    CCTGGTGGCT GTCTAATTGA GCTCTGCATC CAGCTCAGCA TCATCATGTT GGGGAAGCAG
    CTTATTCAGA ACAACGTCTT CGAGATCGGC ATTCCAAAGC TGAAGAAGTT GTGCCGCACG
    CTAAAAGACA AAGATCGGGA GCCCAAGGAG ATTGTGGAGC AGCCGGGCCG CCGCCAACAG
    TGGCACATGG ACTACGACCT GGAGCCGTTT GACGGACTCA CACCTGAATA CATGGAGATG
    ATCATACAGT TTGGTTTCGT GTCACTCTTT GTGGCCTCAT TCCCGCTGGC TCCGCTATTC
    GCACTCCTAA ACAATGTCAT CGAGATTCGC CTCGACGCCA AGAAGTTTGT CACGGAGCTC
    CGCCGGCCAG ACGCCGTGAG GACCAAAGAC ATTGGCATCT GGTATAACAT CTTGAGCGGT
    TTGGGAAAGT TCTCAGTCAT CATCAATGCC TTTGTCATCT CCTTCACATC TGATTTCATC
    CCTCGCCTGG TCTACCAGTA CATGTACAGC GAGACAGGAA CCATGCACGG CTTCATCAAC
    CACACGCTAT CTTACTTTAA CGTCAGCAAC TTCAAACCAG GAACCATGCC TTCAACATCC
    GACATTACAG TCTGCAGGTA CAAGGACTAC AGAGAGCCTC CATGGTCGAA TGAGCCTTAC
    CAGTTCTCCA AACAGTACTG GTCCGTGCTA GCGGCTCGGT TAGGTTTCGT CATTTTATTC
    CAGAACCTGG TGATAATCAT GAGCATGCTG GTGGCCTGGC TGATCCCGGA CATGCCCAAA
    AACATCAGTG AGCAGTTAAA GAAGGACAAG ACTCTTCTAG TGGACGTCTT CCTGAACGAA
    GAAAAGGAGA AGCTGCAGCT GATCCAGAGC CTGTTCTCCA AAGATCTGAC CAACAAGCAG
    CAGGAAGAGC ACCAGCAGCA GGTTTCGGAT AAAACCTCGC TGCCTCTCCA GCAAAGCCAG
    CCTGGCTTTC TCCCTCCGTC CTCATCCCCT GCCTCTACAG CTCCTCGAAC CCGAGCACGA
    GCCGCCAGCT TCAGTCAGTT CACCCGGCAG ATGTCTATGT CTCCCCGCAG GGACGTCACC
    CAGCACACGG CGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44861
    Clone ID Related Accession (Same CDS sequence) XM_009299978.3 , XM_009299978.2
    Accession Version XM_009299978.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299978.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCGTCA ACAGATCCCG CTCGTATGTT GGGATGGATG ATCTGCATGT TCTGAATGGA 
    GTCAGCACTG CTTTGAGCGA GTCCTCATCA GTTTGCAGTG ATTTAAGGGA TATTTCTGAG
    ATTGAAGTCA TGAACTCCAT CAGTTGCACC TTTGATGACT CGGTTGACTT CATTGAGGAG
    TCTTTGCACC ACAGTGACAA CATGTTGCAG ACGAGTGGCA ACAAGATCCA CGTGGCCAAT
    AACATGATGG ACATGGACCA GGACCCTCGC AAGCGGCTCT ACTTTGCTGA CGGCCGAAGG
    AAAGTGGACT ATGTGCTGGT GTTCCACTAC CGAAAGCGTT CCTCCATCAG GGGGTCTCCT
    AGTCAGCACA GACTGTCCAT CATCTCCAAT GGAAGTTATC ATTTAGGAGT GGAGGAAAAA
    GACCCAGAAG GAGGGAAAGA GGAGCCTGCG GAGGTGGTGG TTGATGTTGG ACCACCGGAC
    CCTGCCGAAG GGGAGAAAAT CATGATCCGG GAAGAGTTTG AGGGCAGTTT AAAGGACGCA
    GGGCTGGAAA TTGAACGTGA TAAAGAGAAC AAGGCTCATG GTCATGCCTT TATCCGGTTA
    CACGCGCCTT GGCAGGTATT GAGCAGAGAG GCAGAGTTTC TCAAGATCAA GGTGCAGACC
    AAGAAGAGTT ACGAGCTGAG GGAAGAAAAA GGTCTGGCCG CCACTATGAA TGAAGTGTGG
    CGGAAGTTAA ATCAACCGTT CCAGCCCAAA GTACCCCATC AGGAGCAAGA GAACAGCCGC
    ACCAAGTTCC TCACCCACTG TTTCTCCCGG GACAAGCTCC ACCTGTACAA CATACAGTCA
    AAGGACACCT TCTTCGACAA TGCCACAAGG AGCCGAATAG TGTATGAAAT CCTCAGACGC
    ACAGCCTGCA CACGGACCTG CCAAACCATG GGTATCACCA CACTCATAGC TAACGGGGTT
    TATGATTCAG CATTCCCATT GCACGATGGT GACTTCCACT CCTCTGGACA AGCCGAGGGA
    AAAAACGAAA GACAACTGTT ACATGACGAA TGGGCAAGAT ATGGTGCTTT TTACAAGTAT
    CAGCCGATCG ATCTCATCAG GAAGTACTTT GGCGAGAAGA TTGGTCTGTA CTTTGCCTGG
    CTTGGTGTGT ATACTCAGCT TCTGATCCCT GCATCTGTGG TGGGCATTAT CGTCTTCTTC
    TACGGCTGGG CCACTGTGGA AACCAACGTA CCTAGCCAAG AGATGTGTGA CGAGACTCAG
    AACTTCACTA TGTGTCCGCT CTGTGATCGG GTGTGTGACT ACTGGCAGCT GAGCACAGCG
    TGCGGTACGG CACGTGCCAG CCATCTCTTT GACAACCCGG CCACCGTGTT CTTCGCCATC
    TTCATGTCCC TCTGGGCTGC GATGTTTCTG GAGCACTGGA AGCGCCGACA GATCAGCCTG
    AACTATAGCT GGGATCTGAC AGGCATGGAG GAGGAGGAGG AGCATCCCAG ACCCAAATAC
    GAAACCATCC TCCTTCAGAA GAGGCAGAGG AAGAAGAAAA ACAAGAAGAA CAAAAACGAG
    ACTGGACATG AGAAGCTAAC ATGGAGGGAC AGACTGCCTG GATATCTAAT CAACATCTCC
    TCCATTCTGT TCATGTTTGG AGTGACGTGC TCTGCAGTCT TCAGCGTCAT CATTTACCGA
    ATCACCATCT CCGCCCTTAT GGCCATGAGC CCAGACCCTG ATGTTAAATC CAACGTGAGG
    GTGACCGTCA CAGCCACTGC TGTCATCATC AACCTGGTGG TCATCCTCAT TTTGGATGAA
    ATCTATGGCA TGGTGGCTGC CTGGCTAACA GAACTGGAGA TCCCGAAAAC TGAGACCAAC
    TTTGAAGAGC GTCTGATTCT GAAGGCTTTC GTCCTGAAGT TCATGAATGC CTATGCACCG
    ATCTTTTACG TGGCCTTCTT CAAAGGGAGG TTTGCGGGCC GCCCTGGTGA CTATGTATAT
    GTCTTTCATG ACTATCGAAT GGAAGAATGT GCTCCTGGTG GCTGTCTAAT TGAGCTCTGC
    ATCCAGCTCA GCATCATCAT GTTGGGGAAG CAGCTTATTC AGAACAACGT CTTCGAGATC
    GGCATTCCAA AGCTGAAGAA GTTGTGCCGC ACGCTAAAAG ACAAAGATCG GGAGCCCAAG
    GAGATTGTGG AGCAGCCGGG CCGCCGCCAA CAGTGGCACA TGGACTACGA CCTGGAGCCG
    TTTGACGGAC TCACACCTGA ATACATGGAG ATGATCATAC AGTTTGGTTT CGTGTCACTC
    TTTGTGGCCT CATTCCCGCT GGCTCCGCTA TTCGCACTCC TAAACAATGT CATCGAGATT
    CGCCTCGACG CCAAGAAGTT TGTCACGGAG CTCCGCCGGC CAGACGCCGT GAGGACCAAA
    GACATTGGCA TCTGGTATAA CATCTTGAGC GGTTTGGGAA AGTTCTCAGT CATCATCAAT
    GCCTTTGTCA TCTCCTTCAC ATCTGATTTC ATCCCTCGCC TGGTCTACCA GTACATGTAC
    AGCGAGACAG GAACCATGCA CGGCTTCATC AACCACACGC TATCTTACTT TAACGTCAGC
    AACTTCAAAC CAGGAACCAT GCCTTCAACA TCCGACATTA CAGTCTGCAG GTACAAGGAC
    TACAGAGAGC CTCCATGGTC GAATGAGCCT TACCAGTTCT CCAAACAGTA CTGGTCCGTG
    CTAGCGGCTC GGTTAGGTTT CGTCATTTTA TTCCAGAACC TGGTGATAAT CATGAGCATG
    CTGGTGGCCT GGCTGATCCC GGACATGCCC AAAAACATCA GTGAGCAGTT AAAGAAGGAC
    AAGACTCTTC TAGTGGACGT CTTCCTGAAC GAAGAAAAGG AGAAGCTGCA GCTGATCCAG
    AGCCTGTTCT CCAAAGATCT GACCAACAAG CAGCAGGAAG AGCACCAGCA GCAGGTTTCG
    GATAAAACCT CGCTGCCTCT CCAGCAAAGC CAGCCTGGCT TTCTCCCTCC GTCCTCATCC
    CCTGCCTCTA CAGCTCCTCG AACCCGAGCA CGAGCCGCCA GCTTCAGTCA GTTCACCCGG
    CAGATGTCTA TGTCTCCCCG CAGGGACGTC ACCCAGCACA CGGCGGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298253.1
    CDS72..3182
    Translation

    Target ORF information:

    RefSeq Version XM_009299978.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 2 (ano2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299978.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCGTCA ACAGATCCCG CTCGTATGTT GGGATGGATG ATCTGCATGT TCTGAATGGA 
    GTCAGCACTG CTTTGAGCGA GTCCTCATCA GTTTGCAGTG ATTTAAGGGA TATTTCTGAG
    ATTGAAGTCA TGAACTCCAT CAGTTGCACC TTTGATGACT CGGTTGACTT CATTGAGGAG
    TCTTTGCACC ACAGTGACAA CATGTTGCAG ACGAGTGGCA ACAAGATCCA CGTGGCCAAT
    AACATGATGG ACATGGACCA GGACCCTCGC AAGCGGCTCT ACTTTGCTGA CGGCCGAAGG
    AAAGTGGACT ATGTGCTGGT GTTCCACTAC CGAAAGCGTT CCTCCATCAG GGGGTCTCCT
    AGTCAGCACA GACTGTCCAT CATCTCCAAT GGAAGTTATC ATTTAGGAGT GGAGGAAAAA
    GACCCAGAAG GAGGGAAAGA GGAGCCTGCG GAGGTGGTGG TTGATGTTGG ACCACCGGAC
    CCTGCCGAAG GGGAGAAAAT CATGATCCGG GAAGAGTTTG AGGGCAGTTT AAAGGACGCA
    GGGCTGGAAA TTGAACGTGA TAAAGAGAAC AAGGCTCATG GTCATGCCTT TATCCGGTTA
    CACGCGCCTT GGCAGGTATT GAGCAGAGAG GCAGAGTTTC TCAAGATCAA GGTGCAGACC
    AAGAAGAGTT ACGAGCTGAG GGAAGAAAAA GGTCTGGCCG CCACTATGAA TGAAGTGTGG
    CGGAAGTTAA ATCAACCGTT CCAGCCCAAA GTACCCCATC AGGAGCAAGA GAACAGCCGC
    ACCAAGTTCC TCACCCACTG TTTCTCCCGG GACAAGCTCC ACCTGTACAA CATACAGTCA
    AAGGACACCT TCTTCGACAA TGCCACAAGG AGCCGAATAG TGTATGAAAT CCTCAGACGC
    ACAGCCTGCA CACGGACCTG CCAAACCATG GGTATCACCA CACTCATAGC TAACGGGGTT
    TATGATTCAG CATTCCCATT GCACGATGGT GACTTCCACT CCTCTGGACA AGCCGAGGGA
    AAAAACGAAA GACAACTGTT ACATGACGAA TGGGCAAGAT ATGGTGCTTT TTACAAGTAT
    CAGCCGATCG ATCTCATCAG GAAGTACTTT GGCGAGAAGA TTGGTCTGTA CTTTGCCTGG
    CTTGGTGTGT ATACTCAGCT TCTGATCCCT GCATCTGTGG TGGGCATTAT CGTCTTCTTC
    TACGGCTGGG CCACTGTGGA AACCAACGTA CCTAGCCAAG AGATGTGTGA CGAGACTCAG
    AACTTCACTA TGTGTCCGCT CTGTGATCGG GTGTGTGACT ACTGGCAGCT GAGCACAGCG
    TGCGGTACGG CACGTGCCAG CCATCTCTTT GACAACCCGG CCACCGTGTT CTTCGCCATC
    TTCATGTCCC TCTGGGCTGC GATGTTTCTG GAGCACTGGA AGCGCCGACA GATCAGCCTG
    AACTATAGCT GGGATCTGAC AGGCATGGAG GAGGAGGAGG AGCATCCCAG ACCCAAATAC
    GAAACCATCC TCCTTCAGAA GAGGCAGAGG AAGAAGAAAA ACAAGAAGAA CAAAAACGAG
    ACTGGACATG AGAAGCTAAC ATGGAGGGAC AGACTGCCTG GATATCTAAT CAACATCTCC
    TCCATTCTGT TCATGTTTGG AGTGACGTGC TCTGCAGTCT TCAGCGTCAT CATTTACCGA
    ATCACCATCT CCGCCCTTAT GGCCATGAGC CCAGACCCTG ATGTTAAATC CAACGTGAGG
    GTGACCGTCA CAGCCACTGC TGTCATCATC AACCTGGTGG TCATCCTCAT TTTGGATGAA
    ATCTATGGCA TGGTGGCTGC CTGGCTAACA GAACTGGAGA TCCCGAAAAC TGAGACCAAC
    TTTGAAGAGC GTCTGATTCT GAAGGCTTTC GTCCTGAAGT TCATGAATGC CTATGCACCG
    ATCTTTTACG TGGCCTTCTT CAAAGGGAGG TTTGCGGGCC GCCCTGGTGA CTATGTATAT
    GTCTTTCATG ACTATCGAAT GGAAGAATGT GCTCCTGGTG GCTGTCTAAT TGAGCTCTGC
    ATCCAGCTCA GCATCATCAT GTTGGGGAAG CAGCTTATTC AGAACAACGT CTTCGAGATC
    GGCATTCCAA AGCTGAAGAA GTTGTGCCGC ACGCTAAAAG ACAAAGATCG GGAGCCCAAG
    GAGATTGTGG AGCAGCCGGG CCGCCGCCAA CAGTGGCACA TGGACTACGA CCTGGAGCCG
    TTTGACGGAC TCACACCTGA ATACATGGAG ATGATCATAC AGTTTGGTTT CGTGTCACTC
    TTTGTGGCCT CATTCCCGCT GGCTCCGCTA TTCGCACTCC TAAACAATGT CATCGAGATT
    CGCCTCGACG CCAAGAAGTT TGTCACGGAG CTCCGCCGGC CAGACGCCGT GAGGACCAAA
    GACATTGGCA TCTGGTATAA CATCTTGAGC GGTTTGGGAA AGTTCTCAGT CATCATCAAT
    GCCTTTGTCA TCTCCTTCAC ATCTGATTTC ATCCCTCGCC TGGTCTACCA GTACATGTAC
    AGCGAGACAG GAACCATGCA CGGCTTCATC AACCACACGC TATCTTACTT TAACGTCAGC
    AACTTCAAAC CAGGAACCAT GCCTTCAACA TCCGACATTA CAGTCTGCAG GTACAAGGAC
    TACAGAGAGC CTCCATGGTC GAATGAGCCT TACCAGTTCT CCAAACAGTA CTGGTCCGTG
    CTAGCGGCTC GGTTAGGTTT CGTCATTTTA TTCCAGAACC TGGTGATAAT CATGAGCATG
    CTGGTGGCCT GGCTGATCCC GGACATGCCC AAAAACATCA GTGAGCAGTT AAAGAAGGAC
    AAGACTCTTC TAGTGGACGT CTTCCTGAAC GAAGAAAAGG AGAAGCTGCA GCTGATCCAG
    AGCCTGTTCT CCAAAGATCT GACCAACAAG CAGCAGGAAG AGCACCAGCA GCAGGTTTCG
    GATAAAACCT CGCTGCCTCT CCAGCAAAGC CAGCCTGGCT TTCTCCCTCC GTCCTCATCC
    CCTGCCTCTA CAGCTCCTCG AACCCGAGCA CGAGCCGCCA GCTTCAGTCA GTTCACCCGG
    CAGATGTCTA TGTCTCCCCG CAGGGACGTC ACCCAGCACA CGGCGGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44861
    Clone ID Related Accession (Same CDS sequence) XM_009299978.3 , XM_009299978.2
    Accession Version XM_009299978.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product anoctamin-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCGTCA ACAGATCCCG CTCGTATGTT GGGATGGATG ATCTGCATGT TCTGAATGGA 
    GTCAGCACTG CTTTGAGCGA GTCCTCATCA GTTTGCAGTG ATTTAAGGGA TATTTCTGAG
    ATTGAAGTCA TGAACTCCAT CAGTTGCACC TTTGATGACT CGGTTGACTT CATTGAGGAG
    TCTTTGCACC ACAGTGACAA CATGTTGCAG ACGAGTGGCA ACAAGATCCA CGTGGCCAAT
    AACATGATGG ACATGGACCA GGACCCTCGC AAGCGGCTCT ACTTTGCTGA CGGCCGAAGG
    AAAGTGGACT ATGTGCTGGT GTTCCACTAC CGAAAGCGTT CCTCCATCAG GGGGTCTCCT
    AGTCAGCACA GACTGTCCAT CATCTCCAAT GGAAGTTATC ATTTAGGAGT GGAGGAAAAA
    GACCCAGAAG GAGGGAAAGA GGAGCCTGCG GAGGTGGTGG TTGATGTTGG ACCACCGGAC
    CCTGCCGAAG GGGAGAAAAT CATGATCCGG GAAGAGTTTG AGGGCAGTTT AAAGGACGCA
    GGGCTGGAAA TTGAACGTGA TAAAGAGAAC AAGGCTCATG GTCATGCCTT TATCCGGTTA
    CACGCGCCTT GGCAGGTATT GAGCAGAGAG GCAGAGTTTC TCAAGATCAA GGTGCAGACC
    AAGAAGAGTT ACGAGCTGAG GGAAGAAAAA GGTCTGGCCG CCACTATGAA TGAAGTGTGG
    CGGAAGTTAA ATCAACCGTT CCAGCCCAAA GTACCCCATC AGGAGCAAGA GAACAGCCGC
    ACCAAGTTCC TCACCCACTG TTTCTCCCGG GACAAGCTCC ACCTGTACAA CATACAGTCA
    AAGGACACCT TCTTCGACAA TGCCACAAGG AGCCGAATAG TGTATGAAAT CCTCAGACGC
    ACAGCCTGCA CACGGACCTG CCAAACCATG GGTATCACCA CACTCATAGC TAACGGGGTT
    TATGATTCAG CATTCCCATT GCACGATGGT GACTTCCACT CCTCTGGACA AGCCGAGGGA
    AAAAACGAAA GACAACTGTT ACATGACGAA TGGGCAAGAT ATGGTGCTTT TTACAAGTAT
    CAGCCGATCG ATCTCATCAG GAAGTACTTT GGCGAGAAGA TTGGTCTGTA CTTTGCCTGG
    CTTGGTGTGT ATACTCAGCT TCTGATCCCT GCATCTGTGG TGGGCATTAT CGTCTTCTTC
    TACGGCTGGG CCACTGTGGA AACCAACGTA CCTAGCCAAG AGATGTGTGA CGAGACTCAG
    AACTTCACTA TGTGTCCGCT CTGTGATCGG GTGTGTGACT ACTGGCAGCT GAGCACAGCG
    TGCGGTACGG CACGTGCCAG CCATCTCTTT GACAACCCGG CCACCGTGTT CTTCGCCATC
    TTCATGTCCC TCTGGGCTGC GATGTTTCTG GAGCACTGGA AGCGCCGACA GATCAGCCTG
    AACTATAGCT GGGATCTGAC AGGCATGGAG GAGGAGGAGG AGCATCCCAG ACCCAAATAC
    GAAACCATCC TCCTTCAGAA GAGGCAGAGG AAGAAGAAAA ACAAGAAGAA CAAAAACGAG
    ACTGGACATG AGAAGCTAAC ATGGAGGGAC AGACTGCCTG GATATCTAAT CAACATCTCC
    TCCATTCTGT TCATGTTTGG AGTGACGTGC TCTGCAGTCT TCAGCGTCAT CATTTACCGA
    ATCACCATCT CCGCCCTTAT GGCCATGAGC CCAGACCCTG ATGTTAAATC CAACGTGAGG
    GTGACCGTCA CAGCCACTGC TGTCATCATC AACCTGGTGG TCATCCTCAT TTTGGATGAA
    ATCTATGGCA TGGTGGCTGC CTGGCTAACA GAACTGGAGA TCCCGAAAAC TGAGACCAAC
    TTTGAAGAGC GTCTGATTCT GAAGGCTTTC GTCCTGAAGT TCATGAATGC CTATGCACCG
    ATCTTTTACG TGGCCTTCTT CAAAGGGAGG TTTGCGGGCC GCCCTGGTGA CTATGTATAT
    GTCTTTCATG ACTATCGAAT GGAAGAATGT GCTCCTGGTG GCTGTCTAAT TGAGCTCTGC
    ATCCAGCTCA GCATCATCAT GTTGGGGAAG CAGCTTATTC AGAACAACGT CTTCGAGATC
    GGCATTCCAA AGCTGAAGAA GTTGTGCCGC ACGCTAAAAG ACAAAGATCG GGAGCCCAAG
    GAGATTGTGG AGCAGCCGGG CCGCCGCCAA CAGTGGCACA TGGACTACGA CCTGGAGCCG
    TTTGACGGAC TCACACCTGA ATACATGGAG ATGATCATAC AGTTTGGTTT CGTGTCACTC
    TTTGTGGCCT CATTCCCGCT GGCTCCGCTA TTCGCACTCC TAAACAATGT CATCGAGATT
    CGCCTCGACG CCAAGAAGTT TGTCACGGAG CTCCGCCGGC CAGACGCCGT GAGGACCAAA
    GACATTGGCA TCTGGTATAA CATCTTGAGC GGTTTGGGAA AGTTCTCAGT CATCATCAAT
    GCCTTTGTCA TCTCCTTCAC ATCTGATTTC ATCCCTCGCC TGGTCTACCA GTACATGTAC
    AGCGAGACAG GAACCATGCA CGGCTTCATC AACCACACGC TATCTTACTT TAACGTCAGC
    AACTTCAAAC CAGGAACCAT GCCTTCAACA TCCGACATTA CAGTCTGCAG GTACAAGGAC
    TACAGAGAGC CTCCATGGTC GAATGAGCCT TACCAGTTCT CCAAACAGTA CTGGTCCGTG
    CTAGCGGCTC GGTTAGGTTT CGTCATTTTA TTCCAGAACC TGGTGATAAT CATGAGCATG
    CTGGTGGCCT GGCTGATCCC GGACATGCCC AAAAACATCA GTGAGCAGTT AAAGAAGGAC
    AAGACTCTTC TAGTGGACGT CTTCCTGAAC GAAGAAAAGG AGAAGCTGCA GCTGATCCAG
    AGCCTGTTCT CCAAAGATCT GACCAACAAG CAGCAGGAAG AGCACCAGCA GCAGGTTTCG
    GATAAAACCT CGCTGCCTCT CCAGCAAAGC CAGCCTGGCT TTCTCCCTCC GTCCTCATCC
    CCTGCCTCTA CAGCTCCTCG AACCCGAGCA CGAGCCGCCA GCTTCAGTCA GTTCACCCGG
    CAGATGTCTA TGTCTCCCCG CAGGGACGTC ACCCAGCACA CGGCGGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298253.1
    CDS48..3158
    Translation

    Target ORF information:

    RefSeq Version XM_009299978.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio anoctamin 2 (ano2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299978.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCGTCA ACAGATCCCG CTCGTATGTT GGGATGGATG ATCTGCATGT TCTGAATGGA 
    GTCAGCACTG CTTTGAGCGA GTCCTCATCA GTTTGCAGTG ATTTAAGGGA TATTTCTGAG
    ATTGAAGTCA TGAACTCCAT CAGTTGCACC TTTGATGACT CGGTTGACTT CATTGAGGAG
    TCTTTGCACC ACAGTGACAA CATGTTGCAG ACGAGTGGCA ACAAGATCCA CGTGGCCAAT
    AACATGATGG ACATGGACCA GGACCCTCGC AAGCGGCTCT ACTTTGCTGA CGGCCGAAGG
    AAAGTGGACT ATGTGCTGGT GTTCCACTAC CGAAAGCGTT CCTCCATCAG GGGGTCTCCT
    AGTCAGCACA GACTGTCCAT CATCTCCAAT GGAAGTTATC ATTTAGGAGT GGAGGAAAAA
    GACCCAGAAG GAGGGAAAGA GGAGCCTGCG GAGGTGGTGG TTGATGTTGG ACCACCGGAC
    CCTGCCGAAG GGGAGAAAAT CATGATCCGG GAAGAGTTTG AGGGCAGTTT AAAGGACGCA
    GGGCTGGAAA TTGAACGTGA TAAAGAGAAC AAGGCTCATG GTCATGCCTT TATCCGGTTA
    CACGCGCCTT GGCAGGTATT GAGCAGAGAG GCAGAGTTTC TCAAGATCAA GGTGCAGACC
    AAGAAGAGTT ACGAGCTGAG GGAAGAAAAA GGTCTGGCCG CCACTATGAA TGAAGTGTGG
    CGGAAGTTAA ATCAACCGTT CCAGCCCAAA GTACCCCATC AGGAGCAAGA GAACAGCCGC
    ACCAAGTTCC TCACCCACTG TTTCTCCCGG GACAAGCTCC ACCTGTACAA CATACAGTCA
    AAGGACACCT TCTTCGACAA TGCCACAAGG AGCCGAATAG TGTATGAAAT CCTCAGACGC
    ACAGCCTGCA CACGGACCTG CCAAACCATG GGTATCACCA CACTCATAGC TAACGGGGTT
    TATGATTCAG CATTCCCATT GCACGATGGT GACTTCCACT CCTCTGGACA AGCCGAGGGA
    AAAAACGAAA GACAACTGTT ACATGACGAA TGGGCAAGAT ATGGTGCTTT TTACAAGTAT
    CAGCCGATCG ATCTCATCAG GAAGTACTTT GGCGAGAAGA TTGGTCTGTA CTTTGCCTGG
    CTTGGTGTGT ATACTCAGCT TCTGATCCCT GCATCTGTGG TGGGCATTAT CGTCTTCTTC
    TACGGCTGGG CCACTGTGGA AACCAACGTA CCTAGCCAAG AGATGTGTGA CGAGACTCAG
    AACTTCACTA TGTGTCCGCT CTGTGATCGG GTGTGTGACT ACTGGCAGCT GAGCACAGCG
    TGCGGTACGG CACGTGCCAG CCATCTCTTT GACAACCCGG CCACCGTGTT CTTCGCCATC
    TTCATGTCCC TCTGGGCTGC GATGTTTCTG GAGCACTGGA AGCGCCGACA GATCAGCCTG
    AACTATAGCT GGGATCTGAC AGGCATGGAG GAGGAGGAGG AGCATCCCAG ACCCAAATAC
    GAAACCATCC TCCTTCAGAA GAGGCAGAGG AAGAAGAAAA ACAAGAAGAA CAAAAACGAG
    ACTGGACATG AGAAGCTAAC ATGGAGGGAC AGACTGCCTG GATATCTAAT CAACATCTCC
    TCCATTCTGT TCATGTTTGG AGTGACGTGC TCTGCAGTCT TCAGCGTCAT CATTTACCGA
    ATCACCATCT CCGCCCTTAT GGCCATGAGC CCAGACCCTG ATGTTAAATC CAACGTGAGG
    GTGACCGTCA CAGCCACTGC TGTCATCATC AACCTGGTGG TCATCCTCAT TTTGGATGAA
    ATCTATGGCA TGGTGGCTGC CTGGCTAACA GAACTGGAGA TCCCGAAAAC TGAGACCAAC
    TTTGAAGAGC GTCTGATTCT GAAGGCTTTC GTCCTGAAGT TCATGAATGC CTATGCACCG
    ATCTTTTACG TGGCCTTCTT CAAAGGGAGG TTTGCGGGCC GCCCTGGTGA CTATGTATAT
    GTCTTTCATG ACTATCGAAT GGAAGAATGT GCTCCTGGTG GCTGTCTAAT TGAGCTCTGC
    ATCCAGCTCA GCATCATCAT GTTGGGGAAG CAGCTTATTC AGAACAACGT CTTCGAGATC
    GGCATTCCAA AGCTGAAGAA GTTGTGCCGC ACGCTAAAAG ACAAAGATCG GGAGCCCAAG
    GAGATTGTGG AGCAGCCGGG CCGCCGCCAA CAGTGGCACA TGGACTACGA CCTGGAGCCG
    TTTGACGGAC TCACACCTGA ATACATGGAG ATGATCATAC AGTTTGGTTT CGTGTCACTC
    TTTGTGGCCT CATTCCCGCT GGCTCCGCTA TTCGCACTCC TAAACAATGT CATCGAGATT
    CGCCTCGACG CCAAGAAGTT TGTCACGGAG CTCCGCCGGC CAGACGCCGT GAGGACCAAA
    GACATTGGCA TCTGGTATAA CATCTTGAGC GGTTTGGGAA AGTTCTCAGT CATCATCAAT
    GCCTTTGTCA TCTCCTTCAC ATCTGATTTC ATCCCTCGCC TGGTCTACCA GTACATGTAC
    AGCGAGACAG GAACCATGCA CGGCTTCATC AACCACACGC TATCTTACTT TAACGTCAGC
    AACTTCAAAC CAGGAACCAT GCCTTCAACA TCCGACATTA CAGTCTGCAG GTACAAGGAC
    TACAGAGAGC CTCCATGGTC GAATGAGCCT TACCAGTTCT CCAAACAGTA CTGGTCCGTG
    CTAGCGGCTC GGTTAGGTTT CGTCATTTTA TTCCAGAACC TGGTGATAAT CATGAGCATG
    CTGGTGGCCT GGCTGATCCC GGACATGCCC AAAAACATCA GTGAGCAGTT AAAGAAGGAC
    AAGACTCTTC TAGTGGACGT CTTCCTGAAC GAAGAAAAGG AGAAGCTGCA GCTGATCCAG
    AGCCTGTTCT CCAAAGATCT GACCAACAAG CAGCAGGAAG AGCACCAGCA GCAGGTTTCG
    GATAAAACCT CGCTGCCTCT CCAGCAAAGC CAGCCTGGCT TTCTCCCTCC GTCCTCATCC
    CCTGCCTCTA CAGCTCCTCG AACCCGAGCA CGAGCCGCCA GCTTCAGTCA GTTCACCCGG
    CAGATGTCTA TGTCTCCCCG CAGGGACGTC ACCCAGCACA CGGCGGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.