gbf1 cDNA ORF clone, Danio rerio(zebrafish)

The following gbf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gbf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa44849 XM_009307103.3
Latest version!
Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44850 XM_009307105.2
Latest version!
Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44851 XM_009307106.3
Latest version!
Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44852 XM_009307107.3
Latest version!
Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44853 XM_009307108.3
Latest version!
Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
ODa44852 XM_009307107.2 Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44853 XM_009307108.2 Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44849 XM_009307103.2 Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44851 XM_009307106.2 Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa44849
    Clone ID Related Accession (Same CDS sequence) XM_009307103.2 , XM_009307103.3
    Accession Version XM_009307103.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGGTT
    AGTCTGGATT TGTTAGACCT GATGCACACA CTGCACACGC GAGCAGCCGG CATCTACAGC
    TCCTGGGCGG AGGAACAGCG CCATCTAGAG GATGCAGGAA AGAAGATCGA GGCAGACTCT
    CAAACGCTGT GGACCAGCTG CTGGTGTCCG CTCCTTCAAG GTATGGCTTG GTTGTGCTGT
    GACGCACGTA GACAAGTCCG CATGCAAGCA CTCACTTACC TGCAAAGGGC ACTTCTGGTC
    CATGATCTAC AAACACTGGA TGCGGTGGAG TGGGAGTCCT GCTTCAATAA GGTCCTGTTT
    CCTCTGCTGA CCAAACTTTT GGAAAATATC AGTCCAGCTG ATGTGGGTGG CATGGAGGAG
    ACCAGGATGA GGGCCTGCAC ACTGCTTTCC AAGGTGTTCC TGCAGCATCT CTCTCCTCTG
    CTCTCTCTGC CCACGTTTGC TGCCCTCTGG CTCACCATTC TGGACTTCAT GGACAAGTAC
    ATGCACGCTG GATCCAGCGA CCTTCTGCTG GAGGCCATCC CTGAATCCCT GAAAAACATG
    CTGCTGGTAA TGGACACAGC AGGGATCTTT CATAGCGCAG ACTCCCGCAC AGGATACTCA
    GACCTGTGGG AAATCACCTG GGAGAGAATA GTCTGTTTCC TGCCCCGTCT AAAGGAGGAG
    CTCTTCAAAC AGGCTGTCAT TCCAGAGCCT GTGTGTAATG TTCCTGTGGA GCCCGTCCCG
    CCTCCTGTTG CTGTTCCTCC AGCCGCTCCC ACTGTTCCAG CTCCATCACC AGTGCCAGCA
    GCCCCTGCAG AGCCAAATAT CCCCAGCAGA CCCTCATCGC CTCAAGACAT GCCGACACCG
    AGTGCCAACG GTGATCGAAC AGACAGATCA TCTCCAGTTC CTCCCTCAAC ACCTCCGCTA
    CCCACTCCAG CCAAGAAGAG TCCTCCAGAG TCCCCGCCAC CTCTCAGCCA GTCCCCGCTC
    ATCCTCCAGC CCCTCGCCTC CCCTTTGCAG GTGGGCGTCC CCCCTATGAC CCTGCCAATC
    ATTCTGAACC CCGCCCTCAT AGAGGCCACC TCGCCCGTGC CCCTGCTGCC CCGCCCCTCC
    GCAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305378.1
    CDS135..5723
    Translation

    Target ORF information:

    RefSeq Version XM_009307103.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307103.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGGTT
    AGTCTGGATT TGTTAGACCT GATGCACACA CTGCACACGC GAGCAGCCGG CATCTACAGC
    TCCTGGGCGG AGGAACAGCG CCATCTAGAG GATGCAGGAA AGAAGATCGA GGCAGACTCT
    CAAACGCTGT GGACCAGCTG CTGGTGTCCG CTCCTTCAAG GTATGGCTTG GTTGTGCTGT
    GACGCACGTA GACAAGTCCG CATGCAAGCA CTCACTTACC TGCAAAGGGC ACTTCTGGTC
    CATGATCTAC AAACACTGGA TGCGGTGGAG TGGGAGTCCT GCTTCAATAA GGTCCTGTTT
    CCTCTGCTGA CCAAACTTTT GGAAAATATC AGTCCAGCTG ATGTGGGTGG CATGGAGGAG
    ACCAGGATGA GGGCCTGCAC ACTGCTTTCC AAGGTGTTCC TGCAGCATCT CTCTCCTCTG
    CTCTCTCTGC CCACGTTTGC TGCCCTCTGG CTCACCATTC TGGACTTCAT GGACAAGTAC
    ATGCACGCTG GATCCAGCGA CCTTCTGCTG GAGGCCATCC CTGAATCCCT GAAAAACATG
    CTGCTGGTAA TGGACACAGC AGGGATCTTT CATAGCGCAG ACTCCCGCAC AGGATACTCA
    GACCTGTGGG AAATCACCTG GGAGAGAATA GTCTGTTTCC TGCCCCGTCT AAAGGAGGAG
    CTCTTCAAAC AGGCTGTCAT TCCAGAGCCT GTGTGTAATG TTCCTGTGGA GCCCGTCCCG
    CCTCCTGTTG CTGTTCCTCC AGCCGCTCCC ACTGTTCCAG CTCCATCACC AGTGCCAGCA
    GCCCCTGCAG AGCCAAATAT CCCCAGCAGA CCCTCATCGC CTCAAGACAT GCCGACACCG
    AGTGCCAACG GTGATCGAAC AGACAGATCA TCTCCAGTTC CTCCCTCAAC ACCTCCGCTA
    CCCACTCCAG CCAAGAAGAG TCCTCCAGAG TCCCCGCCAC CTCTCAGCCA GTCCCCGCTC
    ATCCTCCAGC CCCTCGCCTC CCCTTTGCAG GTGGGCGTCC CCCCTATGAC CCTGCCAATC
    ATTCTGAACC CCGCCCTCAT AGAGGCCACC TCGCCCGTGC CCCTGCTGCC CCGCCCCTCC
    GCAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44849
    Clone ID Related Accession (Same CDS sequence) XM_009307103.2 , XM_009307103.3
    Accession Version XM_009307103.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307103.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGGTT
    AGTCTGGATT TGTTAGACCT GATGCACACA CTGCACACGC GAGCAGCCGG CATCTACAGC
    TCCTGGGCGG AGGAACAGCG CCATCTAGAG GATGCAGGAA AGAAGATCGA GGCAGACTCT
    CAAACGCTGT GGACCAGCTG CTGGTGTCCG CTCCTTCAAG GTATGGCTTG GTTGTGCTGT
    GACGCACGTA GACAAGTCCG CATGCAAGCA CTCACTTACC TGCAAAGGGC ACTTCTGGTC
    CATGATCTAC AAACACTGGA TGCGGTGGAG TGGGAGTCCT GCTTCAATAA GGTCCTGTTT
    CCTCTGCTGA CCAAACTTTT GGAAAATATC AGTCCAGCTG ATGTGGGTGG CATGGAGGAG
    ACCAGGATGA GGGCCTGCAC ACTGCTTTCC AAGGTGTTCC TGCAGCATCT CTCTCCTCTG
    CTCTCTCTGC CCACGTTTGC TGCCCTCTGG CTCACCATTC TGGACTTCAT GGACAAGTAC
    ATGCACGCTG GATCCAGCGA CCTTCTGCTG GAGGCCATCC CTGAATCCCT GAAAAACATG
    CTGCTGGTAA TGGACACAGC AGGGATCTTT CATAGCGCAG ACTCCCGCAC AGGATACTCA
    GACCTGTGGG AAATCACCTG GGAGAGAATA GTCTGTTTCC TGCCCCGTCT AAAGGAGGAG
    CTCTTCAAAC AGGCTGTCAT TCCAGAGCCT GTGTGTAATG TTCCTGTGGA GCCCGTCCCG
    CCTCCTGTTG CTGTTCCTCC AGCCGCTCCC ACTGTTCCAG CTCCATCACC AGTGCCAGCA
    GCCCCTGCAG AGCCAAATAT CCCCAGCAGA CCCTCATCGC CTCAAGACAT GCCGACACCG
    AGTGCCAACG GTGATCGAAC AGACAGATCA TCTCCAGTTC CTCCCTCAAC ACCTCCGCTA
    CCCACTCCAG CCAAGAAGAG TCCTCCAGAG TCCCCGCCAC CTCTCAGCCA GTCCCCGCTC
    ATCCTCCAGC CCCTCGCCTC CCCTTTGCAG GTGGGCGTCC CCCCTATGAC CCTGCCAATC
    ATTCTGAACC CCGCCCTCAT AGAGGCCACC TCGCCCGTGC CCCTGCTGCC CCGCCCCTCC
    GCAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305378.1
    CDS156..5744
    Translation

    Target ORF information:

    RefSeq Version XM_009307103.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307103.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGGTT
    AGTCTGGATT TGTTAGACCT GATGCACACA CTGCACACGC GAGCAGCCGG CATCTACAGC
    TCCTGGGCGG AGGAACAGCG CCATCTAGAG GATGCAGGAA AGAAGATCGA GGCAGACTCT
    CAAACGCTGT GGACCAGCTG CTGGTGTCCG CTCCTTCAAG GTATGGCTTG GTTGTGCTGT
    GACGCACGTA GACAAGTCCG CATGCAAGCA CTCACTTACC TGCAAAGGGC ACTTCTGGTC
    CATGATCTAC AAACACTGGA TGCGGTGGAG TGGGAGTCCT GCTTCAATAA GGTCCTGTTT
    CCTCTGCTGA CCAAACTTTT GGAAAATATC AGTCCAGCTG ATGTGGGTGG CATGGAGGAG
    ACCAGGATGA GGGCCTGCAC ACTGCTTTCC AAGGTGTTCC TGCAGCATCT CTCTCCTCTG
    CTCTCTCTGC CCACGTTTGC TGCCCTCTGG CTCACCATTC TGGACTTCAT GGACAAGTAC
    ATGCACGCTG GATCCAGCGA CCTTCTGCTG GAGGCCATCC CTGAATCCCT GAAAAACATG
    CTGCTGGTAA TGGACACAGC AGGGATCTTT CATAGCGCAG ACTCCCGCAC AGGATACTCA
    GACCTGTGGG AAATCACCTG GGAGAGAATA GTCTGTTTCC TGCCCCGTCT AAAGGAGGAG
    CTCTTCAAAC AGGCTGTCAT TCCAGAGCCT GTGTGTAATG TTCCTGTGGA GCCCGTCCCG
    CCTCCTGTTG CTGTTCCTCC AGCCGCTCCC ACTGTTCCAG CTCCATCACC AGTGCCAGCA
    GCCCCTGCAG AGCCAAATAT CCCCAGCAGA CCCTCATCGC CTCAAGACAT GCCGACACCG
    AGTGCCAACG GTGATCGAAC AGACAGATCA TCTCCAGTTC CTCCCTCAAC ACCTCCGCTA
    CCCACTCCAG CCAAGAAGAG TCCTCCAGAG TCCCCGCCAC CTCTCAGCCA GTCCCCGCTC
    ATCCTCCAGC CCCTCGCCTC CCCTTTGCAG GTGGGCGTCC CCCCTATGAC CCTGCCAATC
    ATTCTGAACC CCGCCCTCAT AGAGGCCACC TCGCCCGTGC CCCTGCTGCC CCGCCCCTCC
    GCAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44850
    Clone ID Related Accession (Same CDS sequence) XM_009307105.2
    Accession Version XM_009307105.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGGTT
    AGTCTGGATT TGTTAGACCT GATGCACACA CTGCACACGC GAGCAGCCGG CATCTACAGC
    TCCTGGGCGG AGGAACAGCG CCATCTAGAG GATGCAGGAA AGAAGATCGA GGCAGACTCT
    CAAACGCTGT GGACCAGCTG CTGGTGTCCG CTCCTTCAAG GTATGGCTTG GTTGTGCTGT
    GACGCACGTA GACAAGTCCG CATGCAAGCA CTCACTTACC TGCAAAGGGC ACTTCTGGTC
    CATGATCTAC AAACACTGGA TGCGGTGGAG TGGGAGTCCT GCTTCAATAA GGTCCTGTTT
    CCTCTGCTGA CCAAACTTTT GGAAAATATC AGTCCAGCTG ATGTGGGTGG CATGGAGGAG
    ACCAGGATGA GGGCCTGCAC ACTGCTTTCC AAGGTGTTCC TGCAGCATCT CTCTCCTCTG
    CTCTCTCTGC CCACGTTTGC TGCCCTCTGG CTCACCATTC TGGACTTCAT GGACAAGTAC
    ATGCACGCTG GATCCAGCGA CCTTCTGCTG GAGGCCATCC CTGAATCCCT GAAAAACATG
    CTGCTGGTAA TGGACACAGC AGGGATCTTT CATAGCGCAG ACTCCCGCAC AGGATACTCA
    GACCTGTGGG AAATCACCTG GGAGAGAATA GTCTGTTTCC TGCCCCGTCT AAAGGAGGAG
    CTCTTCAAAC AGGCTGTCAT TCCAGAGCCT GTGTGTAATG TTCCTGTGGA GCCCGTCCCG
    CCTCCTGTTG CTGTTCCTCC AGCCGCTCCC ACTGTTCCAG CTCCATCACC AGTGCCAGCA
    GCCCCTGCAG AGCCAAATAT CCCCAGCAGA CCCTCATCGC CTCAAGACAT GCCGACACCG
    AGTGCCAACG GAACAGACAG ATCATCTCCA GTTCCTCCCT CAACACCTCC GCTACCCACT
    CCAGCCAAGA AGAGTCCTCC AGAGTCCCCG CCACCTCTCA GCCAGTCCCC GCTCATCCTC
    CAGCCCCTCG CCTCCCCTTT GCAGGTGGGC GTCCCCCCTA TGACCCTGCC AATCATTCTG
    AACCCCGCCC TCATAGAGGC CACCTCGCCC GTGCCCCTGC TGCCCCGCCC CTCCGCAGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305380.1
    CDS135..5717
    Translation

    Target ORF information:

    RefSeq Version XM_009307105.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307105.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGGTT
    AGTCTGGATT TGTTAGACCT GATGCACACA CTGCACACGC GAGCAGCCGG CATCTACAGC
    TCCTGGGCGG AGGAACAGCG CCATCTAGAG GATGCAGGAA AGAAGATCGA GGCAGACTCT
    CAAACGCTGT GGACCAGCTG CTGGTGTCCG CTCCTTCAAG GTATGGCTTG GTTGTGCTGT
    GACGCACGTA GACAAGTCCG CATGCAAGCA CTCACTTACC TGCAAAGGGC ACTTCTGGTC
    CATGATCTAC AAACACTGGA TGCGGTGGAG TGGGAGTCCT GCTTCAATAA GGTCCTGTTT
    CCTCTGCTGA CCAAACTTTT GGAAAATATC AGTCCAGCTG ATGTGGGTGG CATGGAGGAG
    ACCAGGATGA GGGCCTGCAC ACTGCTTTCC AAGGTGTTCC TGCAGCATCT CTCTCCTCTG
    CTCTCTCTGC CCACGTTTGC TGCCCTCTGG CTCACCATTC TGGACTTCAT GGACAAGTAC
    ATGCACGCTG GATCCAGCGA CCTTCTGCTG GAGGCCATCC CTGAATCCCT GAAAAACATG
    CTGCTGGTAA TGGACACAGC AGGGATCTTT CATAGCGCAG ACTCCCGCAC AGGATACTCA
    GACCTGTGGG AAATCACCTG GGAGAGAATA GTCTGTTTCC TGCCCCGTCT AAAGGAGGAG
    CTCTTCAAAC AGGCTGTCAT TCCAGAGCCT GTGTGTAATG TTCCTGTGGA GCCCGTCCCG
    CCTCCTGTTG CTGTTCCTCC AGCCGCTCCC ACTGTTCCAG CTCCATCACC AGTGCCAGCA
    GCCCCTGCAG AGCCAAATAT CCCCAGCAGA CCCTCATCGC CTCAAGACAT GCCGACACCG
    AGTGCCAACG GAACAGACAG ATCATCTCCA GTTCCTCCCT CAACACCTCC GCTACCCACT
    CCAGCCAAGA AGAGTCCTCC AGAGTCCCCG CCACCTCTCA GCCAGTCCCC GCTCATCCTC
    CAGCCCCTCG CCTCCCCTTT GCAGGTGGGC GTCCCCCCTA TGACCCTGCC AATCATTCTG
    AACCCCGCCC TCATAGAGGC CACCTCGCCC GTGCCCCTGC TGCCCCGCCC CTCCGCAGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44851
    Clone ID Related Accession (Same CDS sequence) XM_009307106.2 , XM_009307106.3
    Accession Version XM_009307106.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGTTG
    TTAGACCTGA TGCACACACT GCACACGCGA GCAGCCGGCA TCTACAGCTC CTGGGCGGAG
    GAACAGCGCC ATCTAGAGGA TGCAGGAAAG AAGATCGAGG CAGACTCTCA AACGCTGTGG
    ACCAGCTGCT GGTGTCCGCT CCTTCAAGGT ATGGCTTGGT TGTGCTGTGA CGCACGTAGA
    CAAGTCCGCA TGCAAGCACT CACTTACCTG CAAAGGGCAC TTCTGGTCCA TGATCTACAA
    ACACTGGATG CGGTGGAGTG GGAGTCCTGC TTCAATAAGG TCCTGTTTCC TCTGCTGACC
    AAACTTTTGG AAAATATCAG TCCAGCTGAT GTGGGTGGCA TGGAGGAGAC CAGGATGAGG
    GCCTGCACAC TGCTTTCCAA GGTGTTCCTG CAGCATCTCT CTCCTCTGCT CTCTCTGCCC
    ACGTTTGCTG CCCTCTGGCT CACCATTCTG GACTTCATGG ACAAGTACAT GCACGCTGGA
    TCCAGCGACC TTCTGCTGGA GGCCATCCCT GAATCCCTGA AAAACATGCT GCTGGTAATG
    GACACAGCAG GGATCTTTCA TAGCGCAGAC TCCCGCACAG GATACTCAGA CCTGTGGGAA
    ATCACCTGGG AGAGAATAGT CTGTTTCCTG CCCCGTCTAA AGGAGGAGCT CTTCAAACAG
    GCTGTCATTC CAGAGCCTGT GTGTAATGTT CCTGTGGAGC CCGTCCCGCC TCCTGTTGCT
    GTTCCTCCAG CCGCTCCCAC TGTTCCAGCT CCATCACCAG TGCCAGCAGC CCCTGCAGAG
    CCAAATATCC CCAGCAGACC CTCATCGCCT CAAGACATGC CGACACCGAG TGCCAACGGT
    GATCGAACAG ACAGATCATC TCCAGTTCCT CCCTCAACAC CTCCGCTACC CACTCCAGCC
    AAGAAGAGTC CTCCAGAGTC CCCGCCACCT CTCAGCCAGT CCCCGCTCAT CCTCCAGCCC
    CTCGCCTCCC CTTTGCAGGT GGGCGTCCCC CCTATGACCC TGCCAATCAT TCTGAACCCC
    GCCCTCATAG AGGCCACCTC GCCCGTGCCC CTGCTGCCCC GCCCCTCCGC AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305381.1
    CDS135..5711
    Translation

    Target ORF information:

    RefSeq Version XM_009307106.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGTTG
    TTAGACCTGA TGCACACACT GCACACGCGA GCAGCCGGCA TCTACAGCTC CTGGGCGGAG
    GAACAGCGCC ATCTAGAGGA TGCAGGAAAG AAGATCGAGG CAGACTCTCA AACGCTGTGG
    ACCAGCTGCT GGTGTCCGCT CCTTCAAGGT ATGGCTTGGT TGTGCTGTGA CGCACGTAGA
    CAAGTCCGCA TGCAAGCACT CACTTACCTG CAAAGGGCAC TTCTGGTCCA TGATCTACAA
    ACACTGGATG CGGTGGAGTG GGAGTCCTGC TTCAATAAGG TCCTGTTTCC TCTGCTGACC
    AAACTTTTGG AAAATATCAG TCCAGCTGAT GTGGGTGGCA TGGAGGAGAC CAGGATGAGG
    GCCTGCACAC TGCTTTCCAA GGTGTTCCTG CAGCATCTCT CTCCTCTGCT CTCTCTGCCC
    ACGTTTGCTG CCCTCTGGCT CACCATTCTG GACTTCATGG ACAAGTACAT GCACGCTGGA
    TCCAGCGACC TTCTGCTGGA GGCCATCCCT GAATCCCTGA AAAACATGCT GCTGGTAATG
    GACACAGCAG GGATCTTTCA TAGCGCAGAC TCCCGCACAG GATACTCAGA CCTGTGGGAA
    ATCACCTGGG AGAGAATAGT CTGTTTCCTG CCCCGTCTAA AGGAGGAGCT CTTCAAACAG
    GCTGTCATTC CAGAGCCTGT GTGTAATGTT CCTGTGGAGC CCGTCCCGCC TCCTGTTGCT
    GTTCCTCCAG CCGCTCCCAC TGTTCCAGCT CCATCACCAG TGCCAGCAGC CCCTGCAGAG
    CCAAATATCC CCAGCAGACC CTCATCGCCT CAAGACATGC CGACACCGAG TGCCAACGGT
    GATCGAACAG ACAGATCATC TCCAGTTCCT CCCTCAACAC CTCCGCTACC CACTCCAGCC
    AAGAAGAGTC CTCCAGAGTC CCCGCCACCT CTCAGCCAGT CCCCGCTCAT CCTCCAGCCC
    CTCGCCTCCC CTTTGCAGGT GGGCGTCCCC CCTATGACCC TGCCAATCAT TCTGAACCCC
    GCCCTCATAG AGGCCACCTC GCCCGTGCCC CTGCTGCCCC GCCCCTCCGC AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44851
    Clone ID Related Accession (Same CDS sequence) XM_009307106.2 , XM_009307106.3
    Accession Version XM_009307106.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGTTG
    TTAGACCTGA TGCACACACT GCACACGCGA GCAGCCGGCA TCTACAGCTC CTGGGCGGAG
    GAACAGCGCC ATCTAGAGGA TGCAGGAAAG AAGATCGAGG CAGACTCTCA AACGCTGTGG
    ACCAGCTGCT GGTGTCCGCT CCTTCAAGGT ATGGCTTGGT TGTGCTGTGA CGCACGTAGA
    CAAGTCCGCA TGCAAGCACT CACTTACCTG CAAAGGGCAC TTCTGGTCCA TGATCTACAA
    ACACTGGATG CGGTGGAGTG GGAGTCCTGC TTCAATAAGG TCCTGTTTCC TCTGCTGACC
    AAACTTTTGG AAAATATCAG TCCAGCTGAT GTGGGTGGCA TGGAGGAGAC CAGGATGAGG
    GCCTGCACAC TGCTTTCCAA GGTGTTCCTG CAGCATCTCT CTCCTCTGCT CTCTCTGCCC
    ACGTTTGCTG CCCTCTGGCT CACCATTCTG GACTTCATGG ACAAGTACAT GCACGCTGGA
    TCCAGCGACC TTCTGCTGGA GGCCATCCCT GAATCCCTGA AAAACATGCT GCTGGTAATG
    GACACAGCAG GGATCTTTCA TAGCGCAGAC TCCCGCACAG GATACTCAGA CCTGTGGGAA
    ATCACCTGGG AGAGAATAGT CTGTTTCCTG CCCCGTCTAA AGGAGGAGCT CTTCAAACAG
    GCTGTCATTC CAGAGCCTGT GTGTAATGTT CCTGTGGAGC CCGTCCCGCC TCCTGTTGCT
    GTTCCTCCAG CCGCTCCCAC TGTTCCAGCT CCATCACCAG TGCCAGCAGC CCCTGCAGAG
    CCAAATATCC CCAGCAGACC CTCATCGCCT CAAGACATGC CGACACCGAG TGCCAACGGT
    GATCGAACAG ACAGATCATC TCCAGTTCCT CCCTCAACAC CTCCGCTACC CACTCCAGCC
    AAGAAGAGTC CTCCAGAGTC CCCGCCACCT CTCAGCCAGT CCCCGCTCAT CCTCCAGCCC
    CTCGCCTCCC CTTTGCAGGT GGGCGTCCCC CCTATGACCC TGCCAATCAT TCTGAACCCC
    GCCCTCATAG AGGCCACCTC GCCCGTGCCC CTGCTGCCCC GCCCCTCCGC AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305381.1
    CDS156..5732
    Translation

    Target ORF information:

    RefSeq Version XM_009307106.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307106.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGG CTTACAATAT CTTATGGAAA
    AACAAACGAG TGCAGCTAAA GATGAGAGCC GGGGGAATGA GCGAGTCGTC CAAGTGGAAG
    AAACAGAAGC GTTCTCCCCG TCCCCCTCGT CACATGGTGC GCAGTCCCTC AGGACAGATG
    GATCCAGCTC AGTCCAGCAC TCTCACTAAC AACCTTTCAG GTGGAGTTCC CTTCATAGAG
    CAGCACAGTG GAGGTTCTTC TACATTTCCT GGTTCCGACA GTGCAGCTTC CTCCATCTCC
    AGCCCCACCA CTGACAGTGG CTTAGACACC GGCTCCAAAA CCACCTCAAA GGAGGATCTC
    ACCGACCTGG ACCAGAACAG CTCAACAGCC ACCACTCCCA TCACACCCTC CGCAGAGCCG
    GGCAGACTGG AATCACAGTC TGAGGGCAGA GGTGTGGAGC GAGCCCAGTC CGCCTCAGTG
    GAGTCCATTC CTGAAGTCCT GGAAGACCGA GACCTGCTGG CTGACCAGTC TGATTCAGCA
    TCTGTCCACG ATATGGACTA TGTGAACCCT AGAGGAGTGC GCTTTACACA GTCTACTCAG
    AAAGAAGGTG CTGCTCTTAT CCCGTATGGT CTGCCATGTT TGAGAGAGCT CTTCCGGTTC
    CTCATCTCTC TCACCAACCC ACACGACCGC CATAACTCCG ATGTGATGAT GCACATGGGC
    CTTCAGCTCT TAAACGTTGC CCTTGAAGCT GCACACATCG CCCCCTACCA GTCATTGCTC
    GGTCTGGTCA AAGATGAACT CTGTAGGCAC CTCATACAGT TGTTAGGTGT AGACCGAATG
    AATCTGTACA CCGCCTCTAT ACGTGTGTGC TTTCTTCTGT TTGAAAGCAT GCGAGAGCAT
    CTAAAGTTCC AACTTGAGAT GTACCTGAAG AAGCTAATGG ACATCATTAC CTCAGAGAAC
    CCGAAGATGC CATATGAAAT GAAGGAGATG GCCCTGGAGG CCATAGTACA GCTCTGGAGA
    ATCCCCAGCT TTGTCACCGA GCTCTACATA AATTACGACT GTGACTTTTA CTGCTCCAAC
    CTGTTTGAGG ATCTCACCAA ATTGCTCTCT AAGAATGCCT TTCCAGTGTC TGGGCAGCTT
    TACACAACCC ATCTCCTGTC ACTTGAAGCC CTGCTGACAG TGATTGACAG CACCGAGGCT
    CACTGTCAGG CCAAAGTGCT CAACAGCGCT ACACAGCAGG AACAGTCCGA ATCCACAACC
    GTAGGAGACA GTTCAGTCAG TACCATCACA GATTCACCCG CAGAAACTGG CAAACCTCAT
    CCCAGTTCTA ATGGACAGAA CTCTGTGGTG TCTGAGACGA GGGCTTCCTG TCCTCCCACC
    AGCGGTCATC TCATGGCAGA GAAGATGAGA TTAGGTAGAC AGGACCAGGA GGAGACGGAC
    ACAGGAGAGA AGAAAATCTC GAAGAAGCCT CATCGTTTCT CCAGCTATCT TCCAGACTCT
    CAGGAACTTC TGGAAATCAA GAACAAGAAG AAGCTTCTGA TCACGGGTAC AGAACAGTTC
    AATCAGAAGC CAAAGAAAGG CATTCAGACC CTGCAGGAGA AAGGCCTGCT CAGCAGCCCC
    ATGGATAATA ACGAAGTAGC CCAATGGCTG AGAGAAAACC CCCGTCTGGA TAAGAAGATG
    ATTGGAGAGT TTATCAGCGA TCGCAGGAAT ACTGACCTGC TGGACAGTTT TGTCAACACG
    TTCACCTTCC AGGGTCTGCG CATAGACGAG GCCCTCCGCT TGTATCTGGA GGCTTTCCGT
    CTTCCTGGCG AAGCGCCTGT CATTCATCGA CTTTTGGAAA CCTTCACTGA CAACTGGCAT
    AAAGTGAACG GTAATCCCTT CCAGACAAAT GACGCTGGCT TCGCCCTGGC TTACGCCGTG
    ATCATGTTAA ACACCGATCA GCACAACCAT AACGTTCGAA AGCAGAACAT CCCCATGACT
    TTGGAGCAAT TCAAGAAGAA TCTCAAAGGT GTGAACGGAG GAAATGACTT TGATCAAGAT
    ATGCTGGAAG ACATCTACAA TGCCATCAAG AACGAGGAGA TTGTGATGCC GGACGAGCAG
    ACGGGGCTGG TGAAGGAGAA CTACGTGTGG AGCGTTCTGC TGCACAGAGG AGCCTCCGCT
    GAAGGGATGT TCCTGCATGT GCCGGACGGC AGTTATGACC GTGACCTCTT CTCCATGACC
    TGGGGACCCA CCATTGCCGC CCTCTCCTAT GTTTTCGACA AGAGCCTGGA TGATACTATT
    ATCGAGAAGG CTATTGCTGG CTTTAGGAAA TGTGCCATGA TCTCTGCACA CTACGGCTTC
    AGTGACGTCT TTGATAACTT GATCATTTCA CTTTGCAAGT TTACTACACT CAGCTCTGAG
    TCAGTAGAGA ACCTACCCAC CGTGTTTGGC AGTAACAGGA AGGCTCAAGT CGCCGCTAAG
    ACTGTCTTCA GCCTGGCGCA TCGTCATGGT GATATTTTAC GAGACGGATG GAAAAACATC
    ATGGACTCCA TGTTGCAGCT TTTCAGGGCT GAGCTTTTAC CCAAGACCAT GGTCGAGGTT
    GAGGACTTTT TGGATCCTAA TGAGAAGATT TCTTTACAAC GTGAAGAGAC GCCATCAAAC
    AGGGGTGAGT CTGCTGTTTT GAGTTTTGTC AGCTGGTTAA CACTGAGCGA ACAATCAGGG
    CTCAGAGGTC CCTCCACTGA GAACCAGGAG GCCAAGCAAG CTGCACTGCT CTGCATAAAG
    CAATGTGATC CAGAAAAGCT GAACACAGAA AGCAAGTTTC TGCAGCTGGA GTCTTTGCAG
    GAGCTCATGA AGGCTCTCAT CTCTGTGACC CCTGATGAGG AAACATATGA TGAAGAAGAT
    GCTGCTTTTT GCTTGGAGAT GCTGCTACGT ATCATTCTTG AAAACAGGGA CAGAGTATCG
    TGTGTTTGGC AAACGGTGCG GGATCATCTG TATCAGTTGT GTGTCCATGC TAATGAGAGC
    TGCTTCCTGG TGGAGAGAGC AGTGGTGGGA TTGCTCAGGC TTGCCATACG CTTGTTGCGC
    AGAGAGGACA TCGGCTCACA GGTTCTCCTC TCCCTTCGCC TGCTGCTGAT GATGAAACCT
    CACGTCCTGT CTAGAGTCAG TCGGGAGGTG GCCTACGGCT TGCATGAGCT GCTTAAAACT
    AACGCTGCCA ACATCCACAG CACTGACGAT TGGTACACTC TCTTTTCCCT CCTGGAGTGC
    ATCGGCGCTG GGATCAAACC TCCGGCTGCT CTGCAGTTCG CCAACACTAA CCCAGACAAT
    GACACAGGCG CGCAGTCAGA CAGTGAACTC AGCTCATATC ATCAGAGTGA AGTTAGTCTT
    GACCGGGGAT ACACGTCCGA TTCTGAGATT TACAACGAGC ATGGCAAATC CAGAATACCC
    CGTTCTGCTA CTGATGTGGA TGTAGGCTGG CTAGTGGTGG GTAAAGATGA CCTGGACGGA
    GCTAAGGCAG CGGTGACCAC ACCTTCAAAG CCCATGAACC ATCCTCTCGT TAACCAGTAC
    AGTTTAACTC TCGGGCAGGA CATGGGTCTG CATGATACCA AGTCCCTCAT CAAGTGCGTG
    GATTCGCTTT CCTTCATCGT ACGCGATGCT GCTCACGTCA CTCCAGAAAA CTTTGAGCTT
    TGTGTTAAAG CCATTCGTGT GTTCGTGGAG GCCAGCCTTA ATGGAGGCTA CCGCACCCAC
    GAGAAGAAGA AGAGCCACAA ATACGACTCC AAATCCAGCC GGCTCCGAAA GAAGCCACGG
    GAGAAGGAAG GTGCATCACG AAGAACCAAA GCGTCCAGTC AGCGGCCCGC CCGTTCCCAT
    AGCGACGATG AGGAGGAGGA GGGCGTGCCG GCCAGTTACC ACACGGTGTC TTTTCAGTTG
    TTAGACCTGA TGCACACACT GCACACGCGA GCAGCCGGCA TCTACAGCTC CTGGGCGGAG
    GAACAGCGCC ATCTAGAGGA TGCAGGAAAG AAGATCGAGG CAGACTCTCA AACGCTGTGG
    ACCAGCTGCT GGTGTCCGCT CCTTCAAGGT ATGGCTTGGT TGTGCTGTGA CGCACGTAGA
    CAAGTCCGCA TGCAAGCACT CACTTACCTG CAAAGGGCAC TTCTGGTCCA TGATCTACAA
    ACACTGGATG CGGTGGAGTG GGAGTCCTGC TTCAATAAGG TCCTGTTTCC TCTGCTGACC
    AAACTTTTGG AAAATATCAG TCCAGCTGAT GTGGGTGGCA TGGAGGAGAC CAGGATGAGG
    GCCTGCACAC TGCTTTCCAA GGTGTTCCTG CAGCATCTCT CTCCTCTGCT CTCTCTGCCC
    ACGTTTGCTG CCCTCTGGCT CACCATTCTG GACTTCATGG ACAAGTACAT GCACGCTGGA
    TCCAGCGACC TTCTGCTGGA GGCCATCCCT GAATCCCTGA AAAACATGCT GCTGGTAATG
    GACACAGCAG GGATCTTTCA TAGCGCAGAC TCCCGCACAG GATACTCAGA CCTGTGGGAA
    ATCACCTGGG AGAGAATAGT CTGTTTCCTG CCCCGTCTAA AGGAGGAGCT CTTCAAACAG
    GCTGTCATTC CAGAGCCTGT GTGTAATGTT CCTGTGGAGC CCGTCCCGCC TCCTGTTGCT
    GTTCCTCCAG CCGCTCCCAC TGTTCCAGCT CCATCACCAG TGCCAGCAGC CCCTGCAGAG
    CCAAATATCC CCAGCAGACC CTCATCGCCT CAAGACATGC CGACACCGAG TGCCAACGGT
    GATCGAACAG ACAGATCATC TCCAGTTCCT CCCTCAACAC CTCCGCTACC CACTCCAGCC
    AAGAAGAGTC CTCCAGAGTC CCCGCCACCT CTCAGCCAGT CCCCGCTCAT CCTCCAGCCC
    CTCGCCTCCC CTTTGCAGGT GGGCGTCCCC CCTATGACCC TGCCAATCAT TCTGAACCCC
    GCCCTCATAG AGGCCACCTC GCCCGTGCCC CTGCTGCCCC GCCCCTCCGC AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44852
    Clone ID Related Accession (Same CDS sequence) XM_009307107.3 , XM_009307107.2
    Accession Version XM_009307107.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307107.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGC TAAAGATGAG AGCCGGGGGA
    ATGAGCGAGT CGTCCAAGTG GAAGAAACAG AAGCGTTCTC CCCGTCCCCC TCGTCACATG
    GTGCGCAGTC CCTCAGGACA GATGGATCCA GCTCAGTCCA GCACTCTCAC TAACAACCTT
    TCAGGTGGAG TTCCCTTCAT AGAGCAGCAC AGTGGAGGTT CTTCTACATT TCCTGGTTCC
    GACAGTGCAG CTTCCTCCAT CTCCAGCCCC ACCACTGACA GTGGCTTAGA CACCGGCTCC
    AAAACCACCT CAAAGGAGGA TCTCACCGAC CTGGACCAGA ACAGCTCAAC AGCCACCACT
    CCCATCACAC CCTCCGCAGA GCCGGGCAGA CTGGAATCAC AGTCTGAGGG CAGAGGTGTG
    GAGCGAGCCC AGTCCGCCTC AGTGGAGTCC ATTCCTGAAG TCCTGGAAGA CCGAGACCTG
    CTGGCTGACC AGTCTGATTC AGCATCTGTC CACGATATGG ACTATGTGAA CCCTAGAGGA
    GTGCGCTTTA CACAGTCTAC TCAGAAAGAA GGTGCTGCTC TTATCCCGTA TGGTCTGCCA
    TGTTTGAGAG AGCTCTTCCG GTTCCTCATC TCTCTCACCA ACCCACACGA CCGCCATAAC
    TCCGATGTGA TGATGCACAT GGGCCTTCAG CTCTTAAACG TTGCCCTTGA AGCTGCACAC
    ATCGCCCCCT ACCAGTCATT GCTCGGTCTG GTCAAAGATG AACTCTGTAG GCACCTCATA
    CAGTTGTTAG GTGTAGACCG AATGAATCTG TACACCGCCT CTATACGTGT GTGCTTTCTT
    CTGTTTGAAA GCATGCGAGA GCATCTAAAG TTCCAACTTG AGATGTACCT GAAGAAGCTA
    ATGGACATCA TTACCTCAGA GAACCCGAAG ATGCCATATG AAATGAAGGA GATGGCCCTG
    GAGGCCATAG TACAGCTCTG GAGAATCCCC AGCTTTGTCA CCGAGCTCTA CATAAATTAC
    GACTGTGACT TTTACTGCTC CAACCTGTTT GAGGATCTCA CCAAATTGCT CTCTAAGAAT
    GCCTTTCCAG TGTCTGGGCA GCTTTACACA ACCCATCTCC TGTCACTTGA AGCCCTGCTG
    ACAGTGATTG ACAGCACCGA GGCTCACTGT CAGGCCAAAG TGCTCAACAG CGCTACACAG
    CAGGAACAGT CCGAATCCAC AACCGTAGGA GACAGTTCAG TCAGTACCAT CACAGATTCA
    CCCGCAGAAA CTGGCAAACC TCATCCCAGT TCTAATGGAC AGAACTCTGT GGTGTCTGAG
    ACGAGGGCTT CCTGTCCTCC CACCAGCGGT CATCTCATGG CAGAGAAGAT GAGATTAGGT
    AGACAGGACC AGGAGGAGAC GGACACAGGA GAGAAGAAAA TCTCGAAGAA GCCTCATCGT
    TTCTCCAGCT ATCTTCCAGA CTCTCAGGAA CTTCTGGAAA TCAAGAACAA GAAGAAGCTT
    CTGATCACGG GTACAGAACA GTTCAATCAG AAGCCAAAGA AAGGCATTCA GACCCTGCAG
    GAGAAAGGCC TGCTCAGCAG CCCCATGGAT AATAACGAAG TAGCCCAATG GCTGAGAGAA
    AACCCCCGTC TGGATAAGAA GATGATTGGA GAGTTTATCA GCGATCGCAG GAATACTGAC
    CTGCTGGACA GTTTTGTCAA CACGTTCACC TTCCAGGGTC TGCGCATAGA CGAGGCCCTC
    CGCTTGTATC TGGAGGCTTT CCGTCTTCCT GGCGAAGCGC CTGTCATTCA TCGACTTTTG
    GAAACCTTCA CTGACAACTG GCATAAAGTG AACGGTAATC CCTTCCAGAC AAATGACGCT
    GGCTTCGCCC TGGCTTACGC CGTGATCATG TTAAACACCG ATCAGCACAA CCATAACGTT
    CGAAAGCAGA ACATCCCCAT GACTTTGGAG CAATTCAAGA AGAATCTCAA AGGTGTGAAC
    GGAGGAAATG ACTTTGATCA AGATATGCTG GAAGACATCT ACAATGCCAT CAAGAACGAG
    GAGATTGTGA TGCCGGACGA GCAGACGGGG CTGGTGAAGG AGAACTACGT GTGGAGCGTT
    CTGCTGCACA GAGGAGCCTC CGCTGAAGGG ATGTTCCTGC ATGTGCCGGA CGGCAGTTAT
    GACCGTGACC TCTTCTCCAT GACCTGGGGA CCCACCATTG CCGCCCTCTC CTATGTTTTC
    GACAAGAGCC TGGATGATAC TATTATCGAG AAGGCTATTG CTGGCTTTAG GAAATGTGCC
    ATGATCTCTG CACACTACGG CTTCAGTGAC GTCTTTGATA ACTTGATCAT TTCACTTTGC
    AAGTTTACTA CACTCAGCTC TGAGTCAGTA GAGAACCTAC CCACCGTGTT TGGCAGTAAC
    AGGAAGGCTC AAGTCGCCGC TAAGACTGTC TTCAGCCTGG CGCATCGTCA TGGTGATATT
    TTACGAGACG GATGGAAAAA CATCATGGAC TCCATGTTGC AGCTTTTCAG GGCTGAGCTT
    TTACCCAAGA CCATGGTCGA GGTTGAGGAC TTTTTGGATC CTAATGAGAA GATTTCTTTA
    CAACGTGAAG AGACGCCATC AAACAGGGGT GAGTCTGCTG TTTTGAGTTT TGTCAGCTGG
    TTAACACTGA GCGAACAATC AGGGCTCAGA GGTCCCTCCA CTGAGAACCA GGAGGCCAAG
    CAAGCTGCAC TGCTCTGCAT AAAGCAATGT GATCCAGAAA AGCTGAACAC AGAAAGCAAG
    TTTCTGCAGC TGGAGTCTTT GCAGGAGCTC ATGAAGGCTC TCATCTCTGT GACCCCTGAT
    GAGGAAACAT ATGATGAAGA AGATGCTGCT TTTTGCTTGG AGATGCTGCT ACGTATCATT
    CTTGAAAACA GGGACAGAGT ATCGTGTGTT TGGCAAACGG TGCGGGATCA TCTGTATCAG
    TTGTGTGTCC ATGCTAATGA GAGCTGCTTC CTGGTGGAGA GAGCAGTGGT GGGATTGCTC
    AGGCTTGCCA TACGCTTGTT GCGCAGAGAG GACATCGGCT CACAGGTTCT CCTCTCCCTT
    CGCCTGCTGC TGATGATGAA ACCTCACGTC CTGTCTAGAG TCAGTCGGGA GGTGGCCTAC
    GGCTTGCATG AGCTGCTTAA AACTAACGCT GCCAACATCC ACAGCACTGA CGATTGGTAC
    ACTCTCTTTT CCCTCCTGGA GTGCATCGGC GCTGGGATCA AACCTCCGGC TGCTCTGCAG
    TTCGCCAACA CTAACCCAGA CAATGACACA GGCGCGCAGT CAGACAGTGA ACTCAGCTCA
    TATCATCAGA GTGAAGTTAG TCTTGACCGG GGATACACGT CCGATTCTGA GATTTACAAC
    GAGCATGGCA AATCCAGAAT ACCCCGTTCT GCTACTGATG TGGATGTAGG CTGGCTAGTG
    GTGGGTAAAG ATGACCTGGA CGGAGCTAAG GCAGCGGTGA CCACACCTTC AAAGCCCATG
    AACCATCCTC TCGTTAACCA GTACAGTTTA ACTCTCGGGC AGGACATGGG TCTGCATGAT
    ACCAAGTCCC TCATCAAGTG CGTGGATTCG CTTTCCTTCA TCGTACGCGA TGCTGCTCAC
    GTCACTCCAG AAAACTTTGA GCTTTGTGTT AAAGCCATTC GTGTGTTCGT GGAGGCCAGC
    CTTAATGGAG GCTACCGCAC CCACGAGAAG AAGAAGAGCC ACAAATACGA CTCCAAATCC
    AGCCGGCTCC GAAAGAAGCC ACGGGAGAAG GAAGGTGCAT CACGAAGAAC CAAAGCGTCC
    AGTCAGCGGC CCGCCCGTTC CCATAGCGAC GATGAGGAGG AGGAGGGCGT GCCGGCCAGT
    TACCACACGG TGTCTTTTCA GGTTAGTCTG GATTTGTTAG ACCTGATGCA CACACTGCAC
    ACGCGAGCAG CCGGCATCTA CAGCTCCTGG GCGGAGGAAC AGCGCCATCT AGAGGATGCA
    GGAAAGAAGA TCGAGGCAGA CTCTCAAACG CTGTGGACCA GCTGCTGGTG TCCGCTCCTT
    CAAGGTATGG CTTGGTTGTG CTGTGACGCA CGTAGACAAG TCCGCATGCA AGCACTCACT
    TACCTGCAAA GGGCACTTCT GGTCCATGAT CTACAAACAC TGGATGCGGT GGAGTGGGAG
    TCCTGCTTCA ATAAGGTCCT GTTTCCTCTG CTGACCAAAC TTTTGGAAAA TATCAGTCCA
    GCTGATGTGG GTGGCATGGA GGAGACCAGG ATGAGGGCCT GCACACTGCT TTCCAAGGTG
    TTCCTGCAGC ATCTCTCTCC TCTGCTCTCT CTGCCCACGT TTGCTGCCCT CTGGCTCACC
    ATTCTGGACT TCATGGACAA GTACATGCAC GCTGGATCCA GCGACCTTCT GCTGGAGGCC
    ATCCCTGAAT CCCTGAAAAA CATGCTGCTG GTAATGGACA CAGCAGGGAT CTTTCATAGC
    GCAGACTCCC GCACAGGATA CTCAGACCTG TGGGAAATCA CCTGGGAGAG AATAGTCTGT
    TTCCTGCCCC GTCTAAAGGA GGAGCTCTTC AAACAGGCTG TCATTCCAGA GCCTGTGTGT
    AATGTTCCTG TGGAGCCCGT CCCGCCTCCT GTTGCTGTTC CTCCAGCCGC TCCCACTGTT
    CCAGCTCCAT CACCAGTGCC AGCAGCCCCT GCAGAGCCAA ATATCCCCAG CAGACCCTCA
    TCGCCTCAAG ACATGCCGAC ACCGAGTGCC AACGGTGATC GAACAGACAG ATCATCTCCA
    GTTCCTCCCT CAACACCTCC GCTACCCACT CCAGCCAAGA AGAGTCCTCC AGAGTCCCCG
    CCACCTCTCA GCCAGTCCCC GCTCATCCTC CAGCCCCTCG CCTCCCCTTT GCAGGTGGGC
    GTCCCCCCTA TGACCCTGCC AATCATTCTG AACCCCGCCC TCATAGAGGC CACCTCGCCC
    GTGCCCCTGC TGCCCCGCCC CTCCGCAGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305382.1
    CDS156..5708
    Translation

    Target ORF information:

    RefSeq Version XM_009307107.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307107.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGC TAAAGATGAG AGCCGGGGGA
    ATGAGCGAGT CGTCCAAGTG GAAGAAACAG AAGCGTTCTC CCCGTCCCCC TCGTCACATG
    GTGCGCAGTC CCTCAGGACA GATGGATCCA GCTCAGTCCA GCACTCTCAC TAACAACCTT
    TCAGGTGGAG TTCCCTTCAT AGAGCAGCAC AGTGGAGGTT CTTCTACATT TCCTGGTTCC
    GACAGTGCAG CTTCCTCCAT CTCCAGCCCC ACCACTGACA GTGGCTTAGA CACCGGCTCC
    AAAACCACCT CAAAGGAGGA TCTCACCGAC CTGGACCAGA ACAGCTCAAC AGCCACCACT
    CCCATCACAC CCTCCGCAGA GCCGGGCAGA CTGGAATCAC AGTCTGAGGG CAGAGGTGTG
    GAGCGAGCCC AGTCCGCCTC AGTGGAGTCC ATTCCTGAAG TCCTGGAAGA CCGAGACCTG
    CTGGCTGACC AGTCTGATTC AGCATCTGTC CACGATATGG ACTATGTGAA CCCTAGAGGA
    GTGCGCTTTA CACAGTCTAC TCAGAAAGAA GGTGCTGCTC TTATCCCGTA TGGTCTGCCA
    TGTTTGAGAG AGCTCTTCCG GTTCCTCATC TCTCTCACCA ACCCACACGA CCGCCATAAC
    TCCGATGTGA TGATGCACAT GGGCCTTCAG CTCTTAAACG TTGCCCTTGA AGCTGCACAC
    ATCGCCCCCT ACCAGTCATT GCTCGGTCTG GTCAAAGATG AACTCTGTAG GCACCTCATA
    CAGTTGTTAG GTGTAGACCG AATGAATCTG TACACCGCCT CTATACGTGT GTGCTTTCTT
    CTGTTTGAAA GCATGCGAGA GCATCTAAAG TTCCAACTTG AGATGTACCT GAAGAAGCTA
    ATGGACATCA TTACCTCAGA GAACCCGAAG ATGCCATATG AAATGAAGGA GATGGCCCTG
    GAGGCCATAG TACAGCTCTG GAGAATCCCC AGCTTTGTCA CCGAGCTCTA CATAAATTAC
    GACTGTGACT TTTACTGCTC CAACCTGTTT GAGGATCTCA CCAAATTGCT CTCTAAGAAT
    GCCTTTCCAG TGTCTGGGCA GCTTTACACA ACCCATCTCC TGTCACTTGA AGCCCTGCTG
    ACAGTGATTG ACAGCACCGA GGCTCACTGT CAGGCCAAAG TGCTCAACAG CGCTACACAG
    CAGGAACAGT CCGAATCCAC AACCGTAGGA GACAGTTCAG TCAGTACCAT CACAGATTCA
    CCCGCAGAAA CTGGCAAACC TCATCCCAGT TCTAATGGAC AGAACTCTGT GGTGTCTGAG
    ACGAGGGCTT CCTGTCCTCC CACCAGCGGT CATCTCATGG CAGAGAAGAT GAGATTAGGT
    AGACAGGACC AGGAGGAGAC GGACACAGGA GAGAAGAAAA TCTCGAAGAA GCCTCATCGT
    TTCTCCAGCT ATCTTCCAGA CTCTCAGGAA CTTCTGGAAA TCAAGAACAA GAAGAAGCTT
    CTGATCACGG GTACAGAACA GTTCAATCAG AAGCCAAAGA AAGGCATTCA GACCCTGCAG
    GAGAAAGGCC TGCTCAGCAG CCCCATGGAT AATAACGAAG TAGCCCAATG GCTGAGAGAA
    AACCCCCGTC TGGATAAGAA GATGATTGGA GAGTTTATCA GCGATCGCAG GAATACTGAC
    CTGCTGGACA GTTTTGTCAA CACGTTCACC TTCCAGGGTC TGCGCATAGA CGAGGCCCTC
    CGCTTGTATC TGGAGGCTTT CCGTCTTCCT GGCGAAGCGC CTGTCATTCA TCGACTTTTG
    GAAACCTTCA CTGACAACTG GCATAAAGTG AACGGTAATC CCTTCCAGAC AAATGACGCT
    GGCTTCGCCC TGGCTTACGC CGTGATCATG TTAAACACCG ATCAGCACAA CCATAACGTT
    CGAAAGCAGA ACATCCCCAT GACTTTGGAG CAATTCAAGA AGAATCTCAA AGGTGTGAAC
    GGAGGAAATG ACTTTGATCA AGATATGCTG GAAGACATCT ACAATGCCAT CAAGAACGAG
    GAGATTGTGA TGCCGGACGA GCAGACGGGG CTGGTGAAGG AGAACTACGT GTGGAGCGTT
    CTGCTGCACA GAGGAGCCTC CGCTGAAGGG ATGTTCCTGC ATGTGCCGGA CGGCAGTTAT
    GACCGTGACC TCTTCTCCAT GACCTGGGGA CCCACCATTG CCGCCCTCTC CTATGTTTTC
    GACAAGAGCC TGGATGATAC TATTATCGAG AAGGCTATTG CTGGCTTTAG GAAATGTGCC
    ATGATCTCTG CACACTACGG CTTCAGTGAC GTCTTTGATA ACTTGATCAT TTCACTTTGC
    AAGTTTACTA CACTCAGCTC TGAGTCAGTA GAGAACCTAC CCACCGTGTT TGGCAGTAAC
    AGGAAGGCTC AAGTCGCCGC TAAGACTGTC TTCAGCCTGG CGCATCGTCA TGGTGATATT
    TTACGAGACG GATGGAAAAA CATCATGGAC TCCATGTTGC AGCTTTTCAG GGCTGAGCTT
    TTACCCAAGA CCATGGTCGA GGTTGAGGAC TTTTTGGATC CTAATGAGAA GATTTCTTTA
    CAACGTGAAG AGACGCCATC AAACAGGGGT GAGTCTGCTG TTTTGAGTTT TGTCAGCTGG
    TTAACACTGA GCGAACAATC AGGGCTCAGA GGTCCCTCCA CTGAGAACCA GGAGGCCAAG
    CAAGCTGCAC TGCTCTGCAT AAAGCAATGT GATCCAGAAA AGCTGAACAC AGAAAGCAAG
    TTTCTGCAGC TGGAGTCTTT GCAGGAGCTC ATGAAGGCTC TCATCTCTGT GACCCCTGAT
    GAGGAAACAT ATGATGAAGA AGATGCTGCT TTTTGCTTGG AGATGCTGCT ACGTATCATT
    CTTGAAAACA GGGACAGAGT ATCGTGTGTT TGGCAAACGG TGCGGGATCA TCTGTATCAG
    TTGTGTGTCC ATGCTAATGA GAGCTGCTTC CTGGTGGAGA GAGCAGTGGT GGGATTGCTC
    AGGCTTGCCA TACGCTTGTT GCGCAGAGAG GACATCGGCT CACAGGTTCT CCTCTCCCTT
    CGCCTGCTGC TGATGATGAA ACCTCACGTC CTGTCTAGAG TCAGTCGGGA GGTGGCCTAC
    GGCTTGCATG AGCTGCTTAA AACTAACGCT GCCAACATCC ACAGCACTGA CGATTGGTAC
    ACTCTCTTTT CCCTCCTGGA GTGCATCGGC GCTGGGATCA AACCTCCGGC TGCTCTGCAG
    TTCGCCAACA CTAACCCAGA CAATGACACA GGCGCGCAGT CAGACAGTGA ACTCAGCTCA
    TATCATCAGA GTGAAGTTAG TCTTGACCGG GGATACACGT CCGATTCTGA GATTTACAAC
    GAGCATGGCA AATCCAGAAT ACCCCGTTCT GCTACTGATG TGGATGTAGG CTGGCTAGTG
    GTGGGTAAAG ATGACCTGGA CGGAGCTAAG GCAGCGGTGA CCACACCTTC AAAGCCCATG
    AACCATCCTC TCGTTAACCA GTACAGTTTA ACTCTCGGGC AGGACATGGG TCTGCATGAT
    ACCAAGTCCC TCATCAAGTG CGTGGATTCG CTTTCCTTCA TCGTACGCGA TGCTGCTCAC
    GTCACTCCAG AAAACTTTGA GCTTTGTGTT AAAGCCATTC GTGTGTTCGT GGAGGCCAGC
    CTTAATGGAG GCTACCGCAC CCACGAGAAG AAGAAGAGCC ACAAATACGA CTCCAAATCC
    AGCCGGCTCC GAAAGAAGCC ACGGGAGAAG GAAGGTGCAT CACGAAGAAC CAAAGCGTCC
    AGTCAGCGGC CCGCCCGTTC CCATAGCGAC GATGAGGAGG AGGAGGGCGT GCCGGCCAGT
    TACCACACGG TGTCTTTTCA GGTTAGTCTG GATTTGTTAG ACCTGATGCA CACACTGCAC
    ACGCGAGCAG CCGGCATCTA CAGCTCCTGG GCGGAGGAAC AGCGCCATCT AGAGGATGCA
    GGAAAGAAGA TCGAGGCAGA CTCTCAAACG CTGTGGACCA GCTGCTGGTG TCCGCTCCTT
    CAAGGTATGG CTTGGTTGTG CTGTGACGCA CGTAGACAAG TCCGCATGCA AGCACTCACT
    TACCTGCAAA GGGCACTTCT GGTCCATGAT CTACAAACAC TGGATGCGGT GGAGTGGGAG
    TCCTGCTTCA ATAAGGTCCT GTTTCCTCTG CTGACCAAAC TTTTGGAAAA TATCAGTCCA
    GCTGATGTGG GTGGCATGGA GGAGACCAGG ATGAGGGCCT GCACACTGCT TTCCAAGGTG
    TTCCTGCAGC ATCTCTCTCC TCTGCTCTCT CTGCCCACGT TTGCTGCCCT CTGGCTCACC
    ATTCTGGACT TCATGGACAA GTACATGCAC GCTGGATCCA GCGACCTTCT GCTGGAGGCC
    ATCCCTGAAT CCCTGAAAAA CATGCTGCTG GTAATGGACA CAGCAGGGAT CTTTCATAGC
    GCAGACTCCC GCACAGGATA CTCAGACCTG TGGGAAATCA CCTGGGAGAG AATAGTCTGT
    TTCCTGCCCC GTCTAAAGGA GGAGCTCTTC AAACAGGCTG TCATTCCAGA GCCTGTGTGT
    AATGTTCCTG TGGAGCCCGT CCCGCCTCCT GTTGCTGTTC CTCCAGCCGC TCCCACTGTT
    CCAGCTCCAT CACCAGTGCC AGCAGCCCCT GCAGAGCCAA ATATCCCCAG CAGACCCTCA
    TCGCCTCAAG ACATGCCGAC ACCGAGTGCC AACGGTGATC GAACAGACAG ATCATCTCCA
    GTTCCTCCCT CAACACCTCC GCTACCCACT CCAGCCAAGA AGAGTCCTCC AGAGTCCCCG
    CCACCTCTCA GCCAGTCCCC GCTCATCCTC CAGCCCCTCG CCTCCCCTTT GCAGGTGGGC
    GTCCCCCCTA TGACCCTGCC AATCATTCTG AACCCCGCCC TCATAGAGGC CACCTCGCCC
    GTGCCCCTGC TGCCCCGCCC CTCCGCAGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44852
    Clone ID Related Accession (Same CDS sequence) XM_009307107.3 , XM_009307107.2
    Accession Version XM_009307107.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGC TAAAGATGAG AGCCGGGGGA
    ATGAGCGAGT CGTCCAAGTG GAAGAAACAG AAGCGTTCTC CCCGTCCCCC TCGTCACATG
    GTGCGCAGTC CCTCAGGACA GATGGATCCA GCTCAGTCCA GCACTCTCAC TAACAACCTT
    TCAGGTGGAG TTCCCTTCAT AGAGCAGCAC AGTGGAGGTT CTTCTACATT TCCTGGTTCC
    GACAGTGCAG CTTCCTCCAT CTCCAGCCCC ACCACTGACA GTGGCTTAGA CACCGGCTCC
    AAAACCACCT CAAAGGAGGA TCTCACCGAC CTGGACCAGA ACAGCTCAAC AGCCACCACT
    CCCATCACAC CCTCCGCAGA GCCGGGCAGA CTGGAATCAC AGTCTGAGGG CAGAGGTGTG
    GAGCGAGCCC AGTCCGCCTC AGTGGAGTCC ATTCCTGAAG TCCTGGAAGA CCGAGACCTG
    CTGGCTGACC AGTCTGATTC AGCATCTGTC CACGATATGG ACTATGTGAA CCCTAGAGGA
    GTGCGCTTTA CACAGTCTAC TCAGAAAGAA GGTGCTGCTC TTATCCCGTA TGGTCTGCCA
    TGTTTGAGAG AGCTCTTCCG GTTCCTCATC TCTCTCACCA ACCCACACGA CCGCCATAAC
    TCCGATGTGA TGATGCACAT GGGCCTTCAG CTCTTAAACG TTGCCCTTGA AGCTGCACAC
    ATCGCCCCCT ACCAGTCATT GCTCGGTCTG GTCAAAGATG AACTCTGTAG GCACCTCATA
    CAGTTGTTAG GTGTAGACCG AATGAATCTG TACACCGCCT CTATACGTGT GTGCTTTCTT
    CTGTTTGAAA GCATGCGAGA GCATCTAAAG TTCCAACTTG AGATGTACCT GAAGAAGCTA
    ATGGACATCA TTACCTCAGA GAACCCGAAG ATGCCATATG AAATGAAGGA GATGGCCCTG
    GAGGCCATAG TACAGCTCTG GAGAATCCCC AGCTTTGTCA CCGAGCTCTA CATAAATTAC
    GACTGTGACT TTTACTGCTC CAACCTGTTT GAGGATCTCA CCAAATTGCT CTCTAAGAAT
    GCCTTTCCAG TGTCTGGGCA GCTTTACACA ACCCATCTCC TGTCACTTGA AGCCCTGCTG
    ACAGTGATTG ACAGCACCGA GGCTCACTGT CAGGCCAAAG TGCTCAACAG CGCTACACAG
    CAGGAACAGT CCGAATCCAC AACCGTAGGA GACAGTTCAG TCAGTACCAT CACAGATTCA
    CCCGCAGAAA CTGGCAAACC TCATCCCAGT TCTAATGGAC AGAACTCTGT GGTGTCTGAG
    ACGAGGGCTT CCTGTCCTCC CACCAGCGGT CATCTCATGG CAGAGAAGAT GAGATTAGGT
    AGACAGGACC AGGAGGAGAC GGACACAGGA GAGAAGAAAA TCTCGAAGAA GCCTCATCGT
    TTCTCCAGCT ATCTTCCAGA CTCTCAGGAA CTTCTGGAAA TCAAGAACAA GAAGAAGCTT
    CTGATCACGG GTACAGAACA GTTCAATCAG AAGCCAAAGA AAGGCATTCA GACCCTGCAG
    GAGAAAGGCC TGCTCAGCAG CCCCATGGAT AATAACGAAG TAGCCCAATG GCTGAGAGAA
    AACCCCCGTC TGGATAAGAA GATGATTGGA GAGTTTATCA GCGATCGCAG GAATACTGAC
    CTGCTGGACA GTTTTGTCAA CACGTTCACC TTCCAGGGTC TGCGCATAGA CGAGGCCCTC
    CGCTTGTATC TGGAGGCTTT CCGTCTTCCT GGCGAAGCGC CTGTCATTCA TCGACTTTTG
    GAAACCTTCA CTGACAACTG GCATAAAGTG AACGGTAATC CCTTCCAGAC AAATGACGCT
    GGCTTCGCCC TGGCTTACGC CGTGATCATG TTAAACACCG ATCAGCACAA CCATAACGTT
    CGAAAGCAGA ACATCCCCAT GACTTTGGAG CAATTCAAGA AGAATCTCAA AGGTGTGAAC
    GGAGGAAATG ACTTTGATCA AGATATGCTG GAAGACATCT ACAATGCCAT CAAGAACGAG
    GAGATTGTGA TGCCGGACGA GCAGACGGGG CTGGTGAAGG AGAACTACGT GTGGAGCGTT
    CTGCTGCACA GAGGAGCCTC CGCTGAAGGG ATGTTCCTGC ATGTGCCGGA CGGCAGTTAT
    GACCGTGACC TCTTCTCCAT GACCTGGGGA CCCACCATTG CCGCCCTCTC CTATGTTTTC
    GACAAGAGCC TGGATGATAC TATTATCGAG AAGGCTATTG CTGGCTTTAG GAAATGTGCC
    ATGATCTCTG CACACTACGG CTTCAGTGAC GTCTTTGATA ACTTGATCAT TTCACTTTGC
    AAGTTTACTA CACTCAGCTC TGAGTCAGTA GAGAACCTAC CCACCGTGTT TGGCAGTAAC
    AGGAAGGCTC AAGTCGCCGC TAAGACTGTC TTCAGCCTGG CGCATCGTCA TGGTGATATT
    TTACGAGACG GATGGAAAAA CATCATGGAC TCCATGTTGC AGCTTTTCAG GGCTGAGCTT
    TTACCCAAGA CCATGGTCGA GGTTGAGGAC TTTTTGGATC CTAATGAGAA GATTTCTTTA
    CAACGTGAAG AGACGCCATC AAACAGGGGT GAGTCTGCTG TTTTGAGTTT TGTCAGCTGG
    TTAACACTGA GCGAACAATC AGGGCTCAGA GGTCCCTCCA CTGAGAACCA GGAGGCCAAG
    CAAGCTGCAC TGCTCTGCAT AAAGCAATGT GATCCAGAAA AGCTGAACAC AGAAAGCAAG
    TTTCTGCAGC TGGAGTCTTT GCAGGAGCTC ATGAAGGCTC TCATCTCTGT GACCCCTGAT
    GAGGAAACAT ATGATGAAGA AGATGCTGCT TTTTGCTTGG AGATGCTGCT ACGTATCATT
    CTTGAAAACA GGGACAGAGT ATCGTGTGTT TGGCAAACGG TGCGGGATCA TCTGTATCAG
    TTGTGTGTCC ATGCTAATGA GAGCTGCTTC CTGGTGGAGA GAGCAGTGGT GGGATTGCTC
    AGGCTTGCCA TACGCTTGTT GCGCAGAGAG GACATCGGCT CACAGGTTCT CCTCTCCCTT
    CGCCTGCTGC TGATGATGAA ACCTCACGTC CTGTCTAGAG TCAGTCGGGA GGTGGCCTAC
    GGCTTGCATG AGCTGCTTAA AACTAACGCT GCCAACATCC ACAGCACTGA CGATTGGTAC
    ACTCTCTTTT CCCTCCTGGA GTGCATCGGC GCTGGGATCA AACCTCCGGC TGCTCTGCAG
    TTCGCCAACA CTAACCCAGA CAATGACACA GGCGCGCAGT CAGACAGTGA ACTCAGCTCA
    TATCATCAGA GTGAAGTTAG TCTTGACCGG GGATACACGT CCGATTCTGA GATTTACAAC
    GAGCATGGCA AATCCAGAAT ACCCCGTTCT GCTACTGATG TGGATGTAGG CTGGCTAGTG
    GTGGGTAAAG ATGACCTGGA CGGAGCTAAG GCAGCGGTGA CCACACCTTC AAAGCCCATG
    AACCATCCTC TCGTTAACCA GTACAGTTTA ACTCTCGGGC AGGACATGGG TCTGCATGAT
    ACCAAGTCCC TCATCAAGTG CGTGGATTCG CTTTCCTTCA TCGTACGCGA TGCTGCTCAC
    GTCACTCCAG AAAACTTTGA GCTTTGTGTT AAAGCCATTC GTGTGTTCGT GGAGGCCAGC
    CTTAATGGAG GCTACCGCAC CCACGAGAAG AAGAAGAGCC ACAAATACGA CTCCAAATCC
    AGCCGGCTCC GAAAGAAGCC ACGGGAGAAG GAAGGTGCAT CACGAAGAAC CAAAGCGTCC
    AGTCAGCGGC CCGCCCGTTC CCATAGCGAC GATGAGGAGG AGGAGGGCGT GCCGGCCAGT
    TACCACACGG TGTCTTTTCA GGTTAGTCTG GATTTGTTAG ACCTGATGCA CACACTGCAC
    ACGCGAGCAG CCGGCATCTA CAGCTCCTGG GCGGAGGAAC AGCGCCATCT AGAGGATGCA
    GGAAAGAAGA TCGAGGCAGA CTCTCAAACG CTGTGGACCA GCTGCTGGTG TCCGCTCCTT
    CAAGGTATGG CTTGGTTGTG CTGTGACGCA CGTAGACAAG TCCGCATGCA AGCACTCACT
    TACCTGCAAA GGGCACTTCT GGTCCATGAT CTACAAACAC TGGATGCGGT GGAGTGGGAG
    TCCTGCTTCA ATAAGGTCCT GTTTCCTCTG CTGACCAAAC TTTTGGAAAA TATCAGTCCA
    GCTGATGTGG GTGGCATGGA GGAGACCAGG ATGAGGGCCT GCACACTGCT TTCCAAGGTG
    TTCCTGCAGC ATCTCTCTCC TCTGCTCTCT CTGCCCACGT TTGCTGCCCT CTGGCTCACC
    ATTCTGGACT TCATGGACAA GTACATGCAC GCTGGATCCA GCGACCTTCT GCTGGAGGCC
    ATCCCTGAAT CCCTGAAAAA CATGCTGCTG GTAATGGACA CAGCAGGGAT CTTTCATAGC
    GCAGACTCCC GCACAGGATA CTCAGACCTG TGGGAAATCA CCTGGGAGAG AATAGTCTGT
    TTCCTGCCCC GTCTAAAGGA GGAGCTCTTC AAACAGGCTG TCATTCCAGA GCCTGTGTGT
    AATGTTCCTG TGGAGCCCGT CCCGCCTCCT GTTGCTGTTC CTCCAGCCGC TCCCACTGTT
    CCAGCTCCAT CACCAGTGCC AGCAGCCCCT GCAGAGCCAA ATATCCCCAG CAGACCCTCA
    TCGCCTCAAG ACATGCCGAC ACCGAGTGCC AACGGTGATC GAACAGACAG ATCATCTCCA
    GTTCCTCCCT CAACACCTCC GCTACCCACT CCAGCCAAGA AGAGTCCTCC AGAGTCCCCG
    CCACCTCTCA GCCAGTCCCC GCTCATCCTC CAGCCCCTCG CCTCCCCTTT GCAGGTGGGC
    GTCCCCCCTA TGACCCTGCC AATCATTCTG AACCCCGCCC TCATAGAGGC CACCTCGCCC
    GTGCCCCTGC TGCCCCGCCC CTCCGCAGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305382.1
    CDS135..5687
    Translation

    Target ORF information:

    RefSeq Version XM_009307107.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307107.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGC TAAAGATGAG AGCCGGGGGA
    ATGAGCGAGT CGTCCAAGTG GAAGAAACAG AAGCGTTCTC CCCGTCCCCC TCGTCACATG
    GTGCGCAGTC CCTCAGGACA GATGGATCCA GCTCAGTCCA GCACTCTCAC TAACAACCTT
    TCAGGTGGAG TTCCCTTCAT AGAGCAGCAC AGTGGAGGTT CTTCTACATT TCCTGGTTCC
    GACAGTGCAG CTTCCTCCAT CTCCAGCCCC ACCACTGACA GTGGCTTAGA CACCGGCTCC
    AAAACCACCT CAAAGGAGGA TCTCACCGAC CTGGACCAGA ACAGCTCAAC AGCCACCACT
    CCCATCACAC CCTCCGCAGA GCCGGGCAGA CTGGAATCAC AGTCTGAGGG CAGAGGTGTG
    GAGCGAGCCC AGTCCGCCTC AGTGGAGTCC ATTCCTGAAG TCCTGGAAGA CCGAGACCTG
    CTGGCTGACC AGTCTGATTC AGCATCTGTC CACGATATGG ACTATGTGAA CCCTAGAGGA
    GTGCGCTTTA CACAGTCTAC TCAGAAAGAA GGTGCTGCTC TTATCCCGTA TGGTCTGCCA
    TGTTTGAGAG AGCTCTTCCG GTTCCTCATC TCTCTCACCA ACCCACACGA CCGCCATAAC
    TCCGATGTGA TGATGCACAT GGGCCTTCAG CTCTTAAACG TTGCCCTTGA AGCTGCACAC
    ATCGCCCCCT ACCAGTCATT GCTCGGTCTG GTCAAAGATG AACTCTGTAG GCACCTCATA
    CAGTTGTTAG GTGTAGACCG AATGAATCTG TACACCGCCT CTATACGTGT GTGCTTTCTT
    CTGTTTGAAA GCATGCGAGA GCATCTAAAG TTCCAACTTG AGATGTACCT GAAGAAGCTA
    ATGGACATCA TTACCTCAGA GAACCCGAAG ATGCCATATG AAATGAAGGA GATGGCCCTG
    GAGGCCATAG TACAGCTCTG GAGAATCCCC AGCTTTGTCA CCGAGCTCTA CATAAATTAC
    GACTGTGACT TTTACTGCTC CAACCTGTTT GAGGATCTCA CCAAATTGCT CTCTAAGAAT
    GCCTTTCCAG TGTCTGGGCA GCTTTACACA ACCCATCTCC TGTCACTTGA AGCCCTGCTG
    ACAGTGATTG ACAGCACCGA GGCTCACTGT CAGGCCAAAG TGCTCAACAG CGCTACACAG
    CAGGAACAGT CCGAATCCAC AACCGTAGGA GACAGTTCAG TCAGTACCAT CACAGATTCA
    CCCGCAGAAA CTGGCAAACC TCATCCCAGT TCTAATGGAC AGAACTCTGT GGTGTCTGAG
    ACGAGGGCTT CCTGTCCTCC CACCAGCGGT CATCTCATGG CAGAGAAGAT GAGATTAGGT
    AGACAGGACC AGGAGGAGAC GGACACAGGA GAGAAGAAAA TCTCGAAGAA GCCTCATCGT
    TTCTCCAGCT ATCTTCCAGA CTCTCAGGAA CTTCTGGAAA TCAAGAACAA GAAGAAGCTT
    CTGATCACGG GTACAGAACA GTTCAATCAG AAGCCAAAGA AAGGCATTCA GACCCTGCAG
    GAGAAAGGCC TGCTCAGCAG CCCCATGGAT AATAACGAAG TAGCCCAATG GCTGAGAGAA
    AACCCCCGTC TGGATAAGAA GATGATTGGA GAGTTTATCA GCGATCGCAG GAATACTGAC
    CTGCTGGACA GTTTTGTCAA CACGTTCACC TTCCAGGGTC TGCGCATAGA CGAGGCCCTC
    CGCTTGTATC TGGAGGCTTT CCGTCTTCCT GGCGAAGCGC CTGTCATTCA TCGACTTTTG
    GAAACCTTCA CTGACAACTG GCATAAAGTG AACGGTAATC CCTTCCAGAC AAATGACGCT
    GGCTTCGCCC TGGCTTACGC CGTGATCATG TTAAACACCG ATCAGCACAA CCATAACGTT
    CGAAAGCAGA ACATCCCCAT GACTTTGGAG CAATTCAAGA AGAATCTCAA AGGTGTGAAC
    GGAGGAAATG ACTTTGATCA AGATATGCTG GAAGACATCT ACAATGCCAT CAAGAACGAG
    GAGATTGTGA TGCCGGACGA GCAGACGGGG CTGGTGAAGG AGAACTACGT GTGGAGCGTT
    CTGCTGCACA GAGGAGCCTC CGCTGAAGGG ATGTTCCTGC ATGTGCCGGA CGGCAGTTAT
    GACCGTGACC TCTTCTCCAT GACCTGGGGA CCCACCATTG CCGCCCTCTC CTATGTTTTC
    GACAAGAGCC TGGATGATAC TATTATCGAG AAGGCTATTG CTGGCTTTAG GAAATGTGCC
    ATGATCTCTG CACACTACGG CTTCAGTGAC GTCTTTGATA ACTTGATCAT TTCACTTTGC
    AAGTTTACTA CACTCAGCTC TGAGTCAGTA GAGAACCTAC CCACCGTGTT TGGCAGTAAC
    AGGAAGGCTC AAGTCGCCGC TAAGACTGTC TTCAGCCTGG CGCATCGTCA TGGTGATATT
    TTACGAGACG GATGGAAAAA CATCATGGAC TCCATGTTGC AGCTTTTCAG GGCTGAGCTT
    TTACCCAAGA CCATGGTCGA GGTTGAGGAC TTTTTGGATC CTAATGAGAA GATTTCTTTA
    CAACGTGAAG AGACGCCATC AAACAGGGGT GAGTCTGCTG TTTTGAGTTT TGTCAGCTGG
    TTAACACTGA GCGAACAATC AGGGCTCAGA GGTCCCTCCA CTGAGAACCA GGAGGCCAAG
    CAAGCTGCAC TGCTCTGCAT AAAGCAATGT GATCCAGAAA AGCTGAACAC AGAAAGCAAG
    TTTCTGCAGC TGGAGTCTTT GCAGGAGCTC ATGAAGGCTC TCATCTCTGT GACCCCTGAT
    GAGGAAACAT ATGATGAAGA AGATGCTGCT TTTTGCTTGG AGATGCTGCT ACGTATCATT
    CTTGAAAACA GGGACAGAGT ATCGTGTGTT TGGCAAACGG TGCGGGATCA TCTGTATCAG
    TTGTGTGTCC ATGCTAATGA GAGCTGCTTC CTGGTGGAGA GAGCAGTGGT GGGATTGCTC
    AGGCTTGCCA TACGCTTGTT GCGCAGAGAG GACATCGGCT CACAGGTTCT CCTCTCCCTT
    CGCCTGCTGC TGATGATGAA ACCTCACGTC CTGTCTAGAG TCAGTCGGGA GGTGGCCTAC
    GGCTTGCATG AGCTGCTTAA AACTAACGCT GCCAACATCC ACAGCACTGA CGATTGGTAC
    ACTCTCTTTT CCCTCCTGGA GTGCATCGGC GCTGGGATCA AACCTCCGGC TGCTCTGCAG
    TTCGCCAACA CTAACCCAGA CAATGACACA GGCGCGCAGT CAGACAGTGA ACTCAGCTCA
    TATCATCAGA GTGAAGTTAG TCTTGACCGG GGATACACGT CCGATTCTGA GATTTACAAC
    GAGCATGGCA AATCCAGAAT ACCCCGTTCT GCTACTGATG TGGATGTAGG CTGGCTAGTG
    GTGGGTAAAG ATGACCTGGA CGGAGCTAAG GCAGCGGTGA CCACACCTTC AAAGCCCATG
    AACCATCCTC TCGTTAACCA GTACAGTTTA ACTCTCGGGC AGGACATGGG TCTGCATGAT
    ACCAAGTCCC TCATCAAGTG CGTGGATTCG CTTTCCTTCA TCGTACGCGA TGCTGCTCAC
    GTCACTCCAG AAAACTTTGA GCTTTGTGTT AAAGCCATTC GTGTGTTCGT GGAGGCCAGC
    CTTAATGGAG GCTACCGCAC CCACGAGAAG AAGAAGAGCC ACAAATACGA CTCCAAATCC
    AGCCGGCTCC GAAAGAAGCC ACGGGAGAAG GAAGGTGCAT CACGAAGAAC CAAAGCGTCC
    AGTCAGCGGC CCGCCCGTTC CCATAGCGAC GATGAGGAGG AGGAGGGCGT GCCGGCCAGT
    TACCACACGG TGTCTTTTCA GGTTAGTCTG GATTTGTTAG ACCTGATGCA CACACTGCAC
    ACGCGAGCAG CCGGCATCTA CAGCTCCTGG GCGGAGGAAC AGCGCCATCT AGAGGATGCA
    GGAAAGAAGA TCGAGGCAGA CTCTCAAACG CTGTGGACCA GCTGCTGGTG TCCGCTCCTT
    CAAGGTATGG CTTGGTTGTG CTGTGACGCA CGTAGACAAG TCCGCATGCA AGCACTCACT
    TACCTGCAAA GGGCACTTCT GGTCCATGAT CTACAAACAC TGGATGCGGT GGAGTGGGAG
    TCCTGCTTCA ATAAGGTCCT GTTTCCTCTG CTGACCAAAC TTTTGGAAAA TATCAGTCCA
    GCTGATGTGG GTGGCATGGA GGAGACCAGG ATGAGGGCCT GCACACTGCT TTCCAAGGTG
    TTCCTGCAGC ATCTCTCTCC TCTGCTCTCT CTGCCCACGT TTGCTGCCCT CTGGCTCACC
    ATTCTGGACT TCATGGACAA GTACATGCAC GCTGGATCCA GCGACCTTCT GCTGGAGGCC
    ATCCCTGAAT CCCTGAAAAA CATGCTGCTG GTAATGGACA CAGCAGGGAT CTTTCATAGC
    GCAGACTCCC GCACAGGATA CTCAGACCTG TGGGAAATCA CCTGGGAGAG AATAGTCTGT
    TTCCTGCCCC GTCTAAAGGA GGAGCTCTTC AAACAGGCTG TCATTCCAGA GCCTGTGTGT
    AATGTTCCTG TGGAGCCCGT CCCGCCTCCT GTTGCTGTTC CTCCAGCCGC TCCCACTGTT
    CCAGCTCCAT CACCAGTGCC AGCAGCCCCT GCAGAGCCAA ATATCCCCAG CAGACCCTCA
    TCGCCTCAAG ACATGCCGAC ACCGAGTGCC AACGGTGATC GAACAGACAG ATCATCTCCA
    GTTCCTCCCT CAACACCTCC GCTACCCACT CCAGCCAAGA AGAGTCCTCC AGAGTCCCCG
    CCACCTCTCA GCCAGTCCCC GCTCATCCTC CAGCCCCTCG CCTCCCCTTT GCAGGTGGGC
    GTCCCCCCTA TGACCCTGCC AATCATTCTG AACCCCGCCC TCATAGAGGC CACCTCGCCC
    GTGCCCCTGC TGCCCCGCCC CTCCGCAGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44853
    Clone ID Related Accession (Same CDS sequence) XM_009307108.2 , XM_009307108.3
    Accession Version XM_009307108.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGC TAAAGATGAG AGCCGGGGGA
    ATGAGCGAGT CGTCCAAGTG GAAGAAACAG AAGCGTTCTC CCCGTCCCCC TCGTCACATG
    GTGCGCAGTC CCTCAGGACA GATGGATCCA GCTCAGTCCA GCACTCTCAC TAACAACCTT
    TCAGGTGGAG TTCCCTTCAT AGAGCAGCAC AGTGGAGGTT CTTCTACATT TCCTGGTTCC
    GACAGTGCAG CTTCCTCCAT CTCCAGCCCC ACCACTGACA GTGGCTTAGA CACCGGCTCC
    AAAACCACCT CAAAGGAGGA TCTCACCGAC CTGGACCAGA ACAGCTCAAC AGCCACCACT
    CCCATCACAC CCTCCGCAGA GCCGGGCAGA CTGGAATCAC AGTCTGAGGG CAGAGGTGTG
    GAGCGAGCCC AGTCCGCCTC AGTGGAGTCC ATTCCTGAAG TCCTGGAAGA CCGAGACCTG
    CTGGCTGACC AGTCTGATTC AGCATCTGTC CACGATATGG ACTATGTGAA CCCTAGAGGA
    GTGCGCTTTA CACAGTCTAC TCAGAAAGAA GGTGCTGCTC TTATCCCGTA TGGTCTGCCA
    TGTTTGAGAG AGCTCTTCCG GTTCCTCATC TCTCTCACCA ACCCACACGA CCGCCATAAC
    TCCGATGTGA TGATGCACAT GGGCCTTCAG CTCTTAAACG TTGCCCTTGA AGCTGCACAC
    ATCGCCCCCT ACCAGTCATT GCTCGGTCTG GTCAAAGATG AACTCTGTAG GCACCTCATA
    CAGTTGTTAG GTGTAGACCG AATGAATCTG TACACCGCCT CTATACGTGT GTGCTTTCTT
    CTGTTTGAAA GCATGCGAGA GCATCTAAAG TTCCAACTTG AGATGTACCT GAAGAAGCTA
    ATGGACATCA TTACCTCAGA GAACCCGAAG ATGCCATATG AAATGAAGGA GATGGCCCTG
    GAGGCCATAG TACAGCTCTG GAGAATCCCC AGCTTTGTCA CCGAGCTCTA CATAAATTAC
    GACTGTGACT TTTACTGCTC CAACCTGTTT GAGGATCTCA CCAAATTGCT CTCTAAGAAT
    GCCTTTCCAG TGTCTGGGCA GCTTTACACA ACCCATCTCC TGTCACTTGA AGCCCTGCTG
    ACAGTGATTG ACAGCACCGA GGCTCACTGT CAGGCCAAAG TGCTCAACAG CGCTACACAG
    CAGGAACAGT CCGAATCCAC AACCGTAGGA GACAGTTCAG TCAGTACCAT CACAGATTCA
    CCCGCAGAAA CTGGCAAACC TCATCCCAGT TCTAATGGAC AGAACTCTGT GGTGTCTGAG
    ACGAGGGCTT CCTGTCCTCC CACCAGCGGT CATCTCATGG CAGAGAAGAT GAGATTAGGT
    AGACAGGACC AGGAGGAGAC GGACACAGGA GAGAAGAAAA TCTCGAAGAA GCCTCATCGT
    TTCTCCAGCT ATCTTCCAGA CTCTCAGGAA CTTCTGGAAA TCAAGAACAA GAAGAAGCTT
    CTGATCACGG GTACAGAACA GTTCAATCAG AAGCCAAAGA AAGGCATTCA GACCCTGCAG
    GAGAAAGGCC TGCTCAGCAG CCCCATGGAT AATAACGAAG TAGCCCAATG GCTGAGAGAA
    AACCCCCGTC TGGATAAGAA GATGATTGGA GAGTTTATCA GCGATCGCAG GAATACTGAC
    CTGCTGGACA GTTTTGTCAA CACGTTCACC TTCCAGGGTC TGCGCATAGA CGAGGCCCTC
    CGCTTGTATC TGGAGGCTTT CCGTCTTCCT GGCGAAGCGC CTGTCATTCA TCGACTTTTG
    GAAACCTTCA CTGACAACTG GCATAAAGTG AACGGTAATC CCTTCCAGAC AAATGACGCT
    GGCTTCGCCC TGGCTTACGC CGTGATCATG TTAAACACCG ATCAGCACAA CCATAACGTT
    CGAAAGCAGA ACATCCCCAT GACTTTGGAG CAATTCAAGA AGAATCTCAA AGGTGTGAAC
    GGAGGAAATG ACTTTGATCA AGATATGCTG GAAGACATCT ACAATGCCAT CAAGAACGAG
    GAGATTGTGA TGCCGGACGA GCAGACGGGG CTGGTGAAGG AGAACTACGT GTGGAGCGTT
    CTGCTGCACA GAGGAGCCTC CGCTGAAGGG ATGTTCCTGC ATGTGCCGGA CGGCAGTTAT
    GACCGTGACC TCTTCTCCAT GACCTGGGGA CCCACCATTG CCGCCCTCTC CTATGTTTTC
    GACAAGAGCC TGGATGATAC TATTATCGAG AAGGCTATTG CTGGCTTTAG GAAATGTGCC
    ATGATCTCTG CACACTACGG CTTCAGTGAC GTCTTTGATA ACTTGATCAT TTCACTTTGC
    AAGTTTACTA CACTCAGCTC TGAGTCAGTA GAGAACCTAC CCACCGTGTT TGGCAGTAAC
    AGGAAGGCTC AAGTCGCCGC TAAGACTGTC TTCAGCCTGG CGCATCGTCA TGGTGATATT
    TTACGAGACG GATGGAAAAA CATCATGGAC TCCATGTTGC AGCTTTTCAG GGCTGAGCTT
    TTACCCAAGA CCATGGTCGA GGTTGAGGAC TTTTTGGATC CTAATGAGAA GATTTCTTTA
    CAACGTGAAG AGACGCCATC AAACAGGGGT GAGTCTGCTG TTTTGAGTTT TGTCAGCTGG
    TTAACACTGA GCGAACAATC AGGGCTCAGA GGTCCCTCCA CTGAGAACCA GGAGGCCAAG
    CAAGCTGCAC TGCTCTGCAT AAAGCAATGT GATCCAGAAA AGCTGAACAC AGAAAGCAAG
    TTTCTGCAGC TGGAGTCTTT GCAGGAGCTC ATGAAGGCTC TCATCTCTGT GACCCCTGAT
    GAGGAAACAT ATGATGAAGA AGATGCTGCT TTTTGCTTGG AGATGCTGCT ACGTATCATT
    CTTGAAAACA GGGACAGAGT ATCGTGTGTT TGGCAAACGG TGCGGGATCA TCTGTATCAG
    TTGTGTGTCC ATGCTAATGA GAGCTGCTTC CTGGTGGAGA GAGCAGTGGT GGGATTGCTC
    AGGCTTGCCA TACGCTTGTT GCGCAGAGAG GACATCGGCT CACAGGTTCT CCTCTCCCTT
    CGCCTGCTGC TGATGATGAA ACCTCACGTC CTGTCTAGAG TCAGTCGGGA GGTGGCCTAC
    GGCTTGCATG AGCTGCTTAA AACTAACGCT GCCAACATCC ACAGCACTGA CGATTGGTAC
    ACTCTCTTTT CCCTCCTGGA GTGCATCGGC GCTGGGATCA AACCTCCGGC TGCTCTGCAG
    TTCGCCAACA CTAACCCAGA CAATGACACA GGCGCGCAGT CAGACAGTGA ACTCAGCTCA
    TATCATCAGA GTGAAGTTAG TCTTGACCGG GGATACACGT CCGATTCTGA GATTTACAAC
    GAGCATGGCA AATCCAGAAT ACCCCGTTCT GCTACTGATG TGGATGTAGG CTGGCTAGTG
    GTGGGTAAAG ATGACCTGGA CGGAGCTAAG GCAGCGGTGA CCACACCTTC AAAGCCCATG
    AACCATCCTC TCGTTAACCA GTACAGTTTA ACTCTCGGGC AGGACATGGG TCTGCATGAT
    ACCAAGTCCC TCATCAAGTG CGTGGATTCG CTTTCCTTCA TCGTACGCGA TGCTGCTCAC
    GTCACTCCAG AAAACTTTGA GCTTTGTGTT AAAGCCATTC GTGTGTTCGT GGAGGCCAGC
    CTTAATGGAG GCTACCGCAC CCACGAGAAG AAGAAGAGCC ACAAATACGA CTCCAAATCC
    AGCCGGCTCC GAAAGAAGCC ACGGGAGAAG GAAGGTGCAT CACGAAGAAC CAAAGCGTCC
    AGTCAGCGGC CCGCCCGTTC CCATAGCGAC GATGAGGAGG AGGAGGGCGT GCCGGCCAGT
    TACCACACGG TGTCTTTTCA GTTGTTAGAC CTGATGCACA CACTGCACAC GCGAGCAGCC
    GGCATCTACA GCTCCTGGGC GGAGGAACAG CGCCATCTAG AGGATGCAGG AAAGAAGATC
    GAGGCAGACT CTCAAACGCT GTGGACCAGC TGCTGGTGTC CGCTCCTTCA AGGTATGGCT
    TGGTTGTGCT GTGACGCACG TAGACAAGTC CGCATGCAAG CACTCACTTA CCTGCAAAGG
    GCACTTCTGG TCCATGATCT ACAAACACTG GATGCGGTGG AGTGGGAGTC CTGCTTCAAT
    AAGGTCCTGT TTCCTCTGCT GACCAAACTT TTGGAAAATA TCAGTCCAGC TGATGTGGGT
    GGCATGGAGG AGACCAGGAT GAGGGCCTGC ACACTGCTTT CCAAGGTGTT CCTGCAGCAT
    CTCTCTCCTC TGCTCTCTCT GCCCACGTTT GCTGCCCTCT GGCTCACCAT TCTGGACTTC
    ATGGACAAGT ACATGCACGC TGGATCCAGC GACCTTCTGC TGGAGGCCAT CCCTGAATCC
    CTGAAAAACA TGCTGCTGGT AATGGACACA GCAGGGATCT TTCATAGCGC AGACTCCCGC
    ACAGGATACT CAGACCTGTG GGAAATCACC TGGGAGAGAA TAGTCTGTTT CCTGCCCCGT
    CTAAAGGAGG AGCTCTTCAA ACAGGCTGTC ATTCCAGAGC CTGTGTGTAA TGTTCCTGTG
    GAGCCCGTCC CGCCTCCTGT TGCTGTTCCT CCAGCCGCTC CCACTGTTCC AGCTCCATCA
    CCAGTGCCAG CAGCCCCTGC AGAGCCAAAT ATCCCCAGCA GACCCTCATC GCCTCAAGAC
    ATGCCGACAC CGAGTGCCAA CGGTGATCGA ACAGACAGAT CATCTCCAGT TCCTCCCTCA
    ACACCTCCGC TACCCACTCC AGCCAAGAAG AGTCCTCCAG AGTCCCCGCC ACCTCTCAGC
    CAGTCCCCGC TCATCCTCCA GCCCCTCGCC TCCCCTTTGC AGGTGGGCGT CCCCCCTATG
    ACCCTGCCAA TCATTCTGAA CCCCGCCCTC ATAGAGGCCA CCTCGCCCGT GCCCCTGCTG
    CCCCGCCCCT CCGCAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305383.1
    CDS135..5675
    Translation

    Target ORF information:

    RefSeq Version XM_009307108.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307108.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGC TAAAGATGAG AGCCGGGGGA
    ATGAGCGAGT CGTCCAAGTG GAAGAAACAG AAGCGTTCTC CCCGTCCCCC TCGTCACATG
    GTGCGCAGTC CCTCAGGACA GATGGATCCA GCTCAGTCCA GCACTCTCAC TAACAACCTT
    TCAGGTGGAG TTCCCTTCAT AGAGCAGCAC AGTGGAGGTT CTTCTACATT TCCTGGTTCC
    GACAGTGCAG CTTCCTCCAT CTCCAGCCCC ACCACTGACA GTGGCTTAGA CACCGGCTCC
    AAAACCACCT CAAAGGAGGA TCTCACCGAC CTGGACCAGA ACAGCTCAAC AGCCACCACT
    CCCATCACAC CCTCCGCAGA GCCGGGCAGA CTGGAATCAC AGTCTGAGGG CAGAGGTGTG
    GAGCGAGCCC AGTCCGCCTC AGTGGAGTCC ATTCCTGAAG TCCTGGAAGA CCGAGACCTG
    CTGGCTGACC AGTCTGATTC AGCATCTGTC CACGATATGG ACTATGTGAA CCCTAGAGGA
    GTGCGCTTTA CACAGTCTAC TCAGAAAGAA GGTGCTGCTC TTATCCCGTA TGGTCTGCCA
    TGTTTGAGAG AGCTCTTCCG GTTCCTCATC TCTCTCACCA ACCCACACGA CCGCCATAAC
    TCCGATGTGA TGATGCACAT GGGCCTTCAG CTCTTAAACG TTGCCCTTGA AGCTGCACAC
    ATCGCCCCCT ACCAGTCATT GCTCGGTCTG GTCAAAGATG AACTCTGTAG GCACCTCATA
    CAGTTGTTAG GTGTAGACCG AATGAATCTG TACACCGCCT CTATACGTGT GTGCTTTCTT
    CTGTTTGAAA GCATGCGAGA GCATCTAAAG TTCCAACTTG AGATGTACCT GAAGAAGCTA
    ATGGACATCA TTACCTCAGA GAACCCGAAG ATGCCATATG AAATGAAGGA GATGGCCCTG
    GAGGCCATAG TACAGCTCTG GAGAATCCCC AGCTTTGTCA CCGAGCTCTA CATAAATTAC
    GACTGTGACT TTTACTGCTC CAACCTGTTT GAGGATCTCA CCAAATTGCT CTCTAAGAAT
    GCCTTTCCAG TGTCTGGGCA GCTTTACACA ACCCATCTCC TGTCACTTGA AGCCCTGCTG
    ACAGTGATTG ACAGCACCGA GGCTCACTGT CAGGCCAAAG TGCTCAACAG CGCTACACAG
    CAGGAACAGT CCGAATCCAC AACCGTAGGA GACAGTTCAG TCAGTACCAT CACAGATTCA
    CCCGCAGAAA CTGGCAAACC TCATCCCAGT TCTAATGGAC AGAACTCTGT GGTGTCTGAG
    ACGAGGGCTT CCTGTCCTCC CACCAGCGGT CATCTCATGG CAGAGAAGAT GAGATTAGGT
    AGACAGGACC AGGAGGAGAC GGACACAGGA GAGAAGAAAA TCTCGAAGAA GCCTCATCGT
    TTCTCCAGCT ATCTTCCAGA CTCTCAGGAA CTTCTGGAAA TCAAGAACAA GAAGAAGCTT
    CTGATCACGG GTACAGAACA GTTCAATCAG AAGCCAAAGA AAGGCATTCA GACCCTGCAG
    GAGAAAGGCC TGCTCAGCAG CCCCATGGAT AATAACGAAG TAGCCCAATG GCTGAGAGAA
    AACCCCCGTC TGGATAAGAA GATGATTGGA GAGTTTATCA GCGATCGCAG GAATACTGAC
    CTGCTGGACA GTTTTGTCAA CACGTTCACC TTCCAGGGTC TGCGCATAGA CGAGGCCCTC
    CGCTTGTATC TGGAGGCTTT CCGTCTTCCT GGCGAAGCGC CTGTCATTCA TCGACTTTTG
    GAAACCTTCA CTGACAACTG GCATAAAGTG AACGGTAATC CCTTCCAGAC AAATGACGCT
    GGCTTCGCCC TGGCTTACGC CGTGATCATG TTAAACACCG ATCAGCACAA CCATAACGTT
    CGAAAGCAGA ACATCCCCAT GACTTTGGAG CAATTCAAGA AGAATCTCAA AGGTGTGAAC
    GGAGGAAATG ACTTTGATCA AGATATGCTG GAAGACATCT ACAATGCCAT CAAGAACGAG
    GAGATTGTGA TGCCGGACGA GCAGACGGGG CTGGTGAAGG AGAACTACGT GTGGAGCGTT
    CTGCTGCACA GAGGAGCCTC CGCTGAAGGG ATGTTCCTGC ATGTGCCGGA CGGCAGTTAT
    GACCGTGACC TCTTCTCCAT GACCTGGGGA CCCACCATTG CCGCCCTCTC CTATGTTTTC
    GACAAGAGCC TGGATGATAC TATTATCGAG AAGGCTATTG CTGGCTTTAG GAAATGTGCC
    ATGATCTCTG CACACTACGG CTTCAGTGAC GTCTTTGATA ACTTGATCAT TTCACTTTGC
    AAGTTTACTA CACTCAGCTC TGAGTCAGTA GAGAACCTAC CCACCGTGTT TGGCAGTAAC
    AGGAAGGCTC AAGTCGCCGC TAAGACTGTC TTCAGCCTGG CGCATCGTCA TGGTGATATT
    TTACGAGACG GATGGAAAAA CATCATGGAC TCCATGTTGC AGCTTTTCAG GGCTGAGCTT
    TTACCCAAGA CCATGGTCGA GGTTGAGGAC TTTTTGGATC CTAATGAGAA GATTTCTTTA
    CAACGTGAAG AGACGCCATC AAACAGGGGT GAGTCTGCTG TTTTGAGTTT TGTCAGCTGG
    TTAACACTGA GCGAACAATC AGGGCTCAGA GGTCCCTCCA CTGAGAACCA GGAGGCCAAG
    CAAGCTGCAC TGCTCTGCAT AAAGCAATGT GATCCAGAAA AGCTGAACAC AGAAAGCAAG
    TTTCTGCAGC TGGAGTCTTT GCAGGAGCTC ATGAAGGCTC TCATCTCTGT GACCCCTGAT
    GAGGAAACAT ATGATGAAGA AGATGCTGCT TTTTGCTTGG AGATGCTGCT ACGTATCATT
    CTTGAAAACA GGGACAGAGT ATCGTGTGTT TGGCAAACGG TGCGGGATCA TCTGTATCAG
    TTGTGTGTCC ATGCTAATGA GAGCTGCTTC CTGGTGGAGA GAGCAGTGGT GGGATTGCTC
    AGGCTTGCCA TACGCTTGTT GCGCAGAGAG GACATCGGCT CACAGGTTCT CCTCTCCCTT
    CGCCTGCTGC TGATGATGAA ACCTCACGTC CTGTCTAGAG TCAGTCGGGA GGTGGCCTAC
    GGCTTGCATG AGCTGCTTAA AACTAACGCT GCCAACATCC ACAGCACTGA CGATTGGTAC
    ACTCTCTTTT CCCTCCTGGA GTGCATCGGC GCTGGGATCA AACCTCCGGC TGCTCTGCAG
    TTCGCCAACA CTAACCCAGA CAATGACACA GGCGCGCAGT CAGACAGTGA ACTCAGCTCA
    TATCATCAGA GTGAAGTTAG TCTTGACCGG GGATACACGT CCGATTCTGA GATTTACAAC
    GAGCATGGCA AATCCAGAAT ACCCCGTTCT GCTACTGATG TGGATGTAGG CTGGCTAGTG
    GTGGGTAAAG ATGACCTGGA CGGAGCTAAG GCAGCGGTGA CCACACCTTC AAAGCCCATG
    AACCATCCTC TCGTTAACCA GTACAGTTTA ACTCTCGGGC AGGACATGGG TCTGCATGAT
    ACCAAGTCCC TCATCAAGTG CGTGGATTCG CTTTCCTTCA TCGTACGCGA TGCTGCTCAC
    GTCACTCCAG AAAACTTTGA GCTTTGTGTT AAAGCCATTC GTGTGTTCGT GGAGGCCAGC
    CTTAATGGAG GCTACCGCAC CCACGAGAAG AAGAAGAGCC ACAAATACGA CTCCAAATCC
    AGCCGGCTCC GAAAGAAGCC ACGGGAGAAG GAAGGTGCAT CACGAAGAAC CAAAGCGTCC
    AGTCAGCGGC CCGCCCGTTC CCATAGCGAC GATGAGGAGG AGGAGGGCGT GCCGGCCAGT
    TACCACACGG TGTCTTTTCA GTTGTTAGAC CTGATGCACA CACTGCACAC GCGAGCAGCC
    GGCATCTACA GCTCCTGGGC GGAGGAACAG CGCCATCTAG AGGATGCAGG AAAGAAGATC
    GAGGCAGACT CTCAAACGCT GTGGACCAGC TGCTGGTGTC CGCTCCTTCA AGGTATGGCT
    TGGTTGTGCT GTGACGCACG TAGACAAGTC CGCATGCAAG CACTCACTTA CCTGCAAAGG
    GCACTTCTGG TCCATGATCT ACAAACACTG GATGCGGTGG AGTGGGAGTC CTGCTTCAAT
    AAGGTCCTGT TTCCTCTGCT GACCAAACTT TTGGAAAATA TCAGTCCAGC TGATGTGGGT
    GGCATGGAGG AGACCAGGAT GAGGGCCTGC ACACTGCTTT CCAAGGTGTT CCTGCAGCAT
    CTCTCTCCTC TGCTCTCTCT GCCCACGTTT GCTGCCCTCT GGCTCACCAT TCTGGACTTC
    ATGGACAAGT ACATGCACGC TGGATCCAGC GACCTTCTGC TGGAGGCCAT CCCTGAATCC
    CTGAAAAACA TGCTGCTGGT AATGGACACA GCAGGGATCT TTCATAGCGC AGACTCCCGC
    ACAGGATACT CAGACCTGTG GGAAATCACC TGGGAGAGAA TAGTCTGTTT CCTGCCCCGT
    CTAAAGGAGG AGCTCTTCAA ACAGGCTGTC ATTCCAGAGC CTGTGTGTAA TGTTCCTGTG
    GAGCCCGTCC CGCCTCCTGT TGCTGTTCCT CCAGCCGCTC CCACTGTTCC AGCTCCATCA
    CCAGTGCCAG CAGCCCCTGC AGAGCCAAAT ATCCCCAGCA GACCCTCATC GCCTCAAGAC
    ATGCCGACAC CGAGTGCCAA CGGTGATCGA ACAGACAGAT CATCTCCAGT TCCTCCCTCA
    ACACCTCCGC TACCCACTCC AGCCAAGAAG AGTCCTCCAG AGTCCCCGCC ACCTCTCAGC
    CAGTCCCCGC TCATCCTCCA GCCCCTCGCC TCCCCTTTGC AGGTGGGCGT CCCCCCTATG
    ACCCTGCCAA TCATTCTGAA CCCCGCCCTC ATAGAGGCCA CCTCGCCCGT GCCCCTGCTG
    CCCCGCCCCT CCGCAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44853
    Clone ID Related Accession (Same CDS sequence) XM_009307108.2 , XM_009307108.3
    Accession Version XM_009307108.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307108.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGC TAAAGATGAG AGCCGGGGGA
    ATGAGCGAGT CGTCCAAGTG GAAGAAACAG AAGCGTTCTC CCCGTCCCCC TCGTCACATG
    GTGCGCAGTC CCTCAGGACA GATGGATCCA GCTCAGTCCA GCACTCTCAC TAACAACCTT
    TCAGGTGGAG TTCCCTTCAT AGAGCAGCAC AGTGGAGGTT CTTCTACATT TCCTGGTTCC
    GACAGTGCAG CTTCCTCCAT CTCCAGCCCC ACCACTGACA GTGGCTTAGA CACCGGCTCC
    AAAACCACCT CAAAGGAGGA TCTCACCGAC CTGGACCAGA ACAGCTCAAC AGCCACCACT
    CCCATCACAC CCTCCGCAGA GCCGGGCAGA CTGGAATCAC AGTCTGAGGG CAGAGGTGTG
    GAGCGAGCCC AGTCCGCCTC AGTGGAGTCC ATTCCTGAAG TCCTGGAAGA CCGAGACCTG
    CTGGCTGACC AGTCTGATTC AGCATCTGTC CACGATATGG ACTATGTGAA CCCTAGAGGA
    GTGCGCTTTA CACAGTCTAC TCAGAAAGAA GGTGCTGCTC TTATCCCGTA TGGTCTGCCA
    TGTTTGAGAG AGCTCTTCCG GTTCCTCATC TCTCTCACCA ACCCACACGA CCGCCATAAC
    TCCGATGTGA TGATGCACAT GGGCCTTCAG CTCTTAAACG TTGCCCTTGA AGCTGCACAC
    ATCGCCCCCT ACCAGTCATT GCTCGGTCTG GTCAAAGATG AACTCTGTAG GCACCTCATA
    CAGTTGTTAG GTGTAGACCG AATGAATCTG TACACCGCCT CTATACGTGT GTGCTTTCTT
    CTGTTTGAAA GCATGCGAGA GCATCTAAAG TTCCAACTTG AGATGTACCT GAAGAAGCTA
    ATGGACATCA TTACCTCAGA GAACCCGAAG ATGCCATATG AAATGAAGGA GATGGCCCTG
    GAGGCCATAG TACAGCTCTG GAGAATCCCC AGCTTTGTCA CCGAGCTCTA CATAAATTAC
    GACTGTGACT TTTACTGCTC CAACCTGTTT GAGGATCTCA CCAAATTGCT CTCTAAGAAT
    GCCTTTCCAG TGTCTGGGCA GCTTTACACA ACCCATCTCC TGTCACTTGA AGCCCTGCTG
    ACAGTGATTG ACAGCACCGA GGCTCACTGT CAGGCCAAAG TGCTCAACAG CGCTACACAG
    CAGGAACAGT CCGAATCCAC AACCGTAGGA GACAGTTCAG TCAGTACCAT CACAGATTCA
    CCCGCAGAAA CTGGCAAACC TCATCCCAGT TCTAATGGAC AGAACTCTGT GGTGTCTGAG
    ACGAGGGCTT CCTGTCCTCC CACCAGCGGT CATCTCATGG CAGAGAAGAT GAGATTAGGT
    AGACAGGACC AGGAGGAGAC GGACACAGGA GAGAAGAAAA TCTCGAAGAA GCCTCATCGT
    TTCTCCAGCT ATCTTCCAGA CTCTCAGGAA CTTCTGGAAA TCAAGAACAA GAAGAAGCTT
    CTGATCACGG GTACAGAACA GTTCAATCAG AAGCCAAAGA AAGGCATTCA GACCCTGCAG
    GAGAAAGGCC TGCTCAGCAG CCCCATGGAT AATAACGAAG TAGCCCAATG GCTGAGAGAA
    AACCCCCGTC TGGATAAGAA GATGATTGGA GAGTTTATCA GCGATCGCAG GAATACTGAC
    CTGCTGGACA GTTTTGTCAA CACGTTCACC TTCCAGGGTC TGCGCATAGA CGAGGCCCTC
    CGCTTGTATC TGGAGGCTTT CCGTCTTCCT GGCGAAGCGC CTGTCATTCA TCGACTTTTG
    GAAACCTTCA CTGACAACTG GCATAAAGTG AACGGTAATC CCTTCCAGAC AAATGACGCT
    GGCTTCGCCC TGGCTTACGC CGTGATCATG TTAAACACCG ATCAGCACAA CCATAACGTT
    CGAAAGCAGA ACATCCCCAT GACTTTGGAG CAATTCAAGA AGAATCTCAA AGGTGTGAAC
    GGAGGAAATG ACTTTGATCA AGATATGCTG GAAGACATCT ACAATGCCAT CAAGAACGAG
    GAGATTGTGA TGCCGGACGA GCAGACGGGG CTGGTGAAGG AGAACTACGT GTGGAGCGTT
    CTGCTGCACA GAGGAGCCTC CGCTGAAGGG ATGTTCCTGC ATGTGCCGGA CGGCAGTTAT
    GACCGTGACC TCTTCTCCAT GACCTGGGGA CCCACCATTG CCGCCCTCTC CTATGTTTTC
    GACAAGAGCC TGGATGATAC TATTATCGAG AAGGCTATTG CTGGCTTTAG GAAATGTGCC
    ATGATCTCTG CACACTACGG CTTCAGTGAC GTCTTTGATA ACTTGATCAT TTCACTTTGC
    AAGTTTACTA CACTCAGCTC TGAGTCAGTA GAGAACCTAC CCACCGTGTT TGGCAGTAAC
    AGGAAGGCTC AAGTCGCCGC TAAGACTGTC TTCAGCCTGG CGCATCGTCA TGGTGATATT
    TTACGAGACG GATGGAAAAA CATCATGGAC TCCATGTTGC AGCTTTTCAG GGCTGAGCTT
    TTACCCAAGA CCATGGTCGA GGTTGAGGAC TTTTTGGATC CTAATGAGAA GATTTCTTTA
    CAACGTGAAG AGACGCCATC AAACAGGGGT GAGTCTGCTG TTTTGAGTTT TGTCAGCTGG
    TTAACACTGA GCGAACAATC AGGGCTCAGA GGTCCCTCCA CTGAGAACCA GGAGGCCAAG
    CAAGCTGCAC TGCTCTGCAT AAAGCAATGT GATCCAGAAA AGCTGAACAC AGAAAGCAAG
    TTTCTGCAGC TGGAGTCTTT GCAGGAGCTC ATGAAGGCTC TCATCTCTGT GACCCCTGAT
    GAGGAAACAT ATGATGAAGA AGATGCTGCT TTTTGCTTGG AGATGCTGCT ACGTATCATT
    CTTGAAAACA GGGACAGAGT ATCGTGTGTT TGGCAAACGG TGCGGGATCA TCTGTATCAG
    TTGTGTGTCC ATGCTAATGA GAGCTGCTTC CTGGTGGAGA GAGCAGTGGT GGGATTGCTC
    AGGCTTGCCA TACGCTTGTT GCGCAGAGAG GACATCGGCT CACAGGTTCT CCTCTCCCTT
    CGCCTGCTGC TGATGATGAA ACCTCACGTC CTGTCTAGAG TCAGTCGGGA GGTGGCCTAC
    GGCTTGCATG AGCTGCTTAA AACTAACGCT GCCAACATCC ACAGCACTGA CGATTGGTAC
    ACTCTCTTTT CCCTCCTGGA GTGCATCGGC GCTGGGATCA AACCTCCGGC TGCTCTGCAG
    TTCGCCAACA CTAACCCAGA CAATGACACA GGCGCGCAGT CAGACAGTGA ACTCAGCTCA
    TATCATCAGA GTGAAGTTAG TCTTGACCGG GGATACACGT CCGATTCTGA GATTTACAAC
    GAGCATGGCA AATCCAGAAT ACCCCGTTCT GCTACTGATG TGGATGTAGG CTGGCTAGTG
    GTGGGTAAAG ATGACCTGGA CGGAGCTAAG GCAGCGGTGA CCACACCTTC AAAGCCCATG
    AACCATCCTC TCGTTAACCA GTACAGTTTA ACTCTCGGGC AGGACATGGG TCTGCATGAT
    ACCAAGTCCC TCATCAAGTG CGTGGATTCG CTTTCCTTCA TCGTACGCGA TGCTGCTCAC
    GTCACTCCAG AAAACTTTGA GCTTTGTGTT AAAGCCATTC GTGTGTTCGT GGAGGCCAGC
    CTTAATGGAG GCTACCGCAC CCACGAGAAG AAGAAGAGCC ACAAATACGA CTCCAAATCC
    AGCCGGCTCC GAAAGAAGCC ACGGGAGAAG GAAGGTGCAT CACGAAGAAC CAAAGCGTCC
    AGTCAGCGGC CCGCCCGTTC CCATAGCGAC GATGAGGAGG AGGAGGGCGT GCCGGCCAGT
    TACCACACGG TGTCTTTTCA GTTGTTAGAC CTGATGCACA CACTGCACAC GCGAGCAGCC
    GGCATCTACA GCTCCTGGGC GGAGGAACAG CGCCATCTAG AGGATGCAGG AAAGAAGATC
    GAGGCAGACT CTCAAACGCT GTGGACCAGC TGCTGGTGTC CGCTCCTTCA AGGTATGGCT
    TGGTTGTGCT GTGACGCACG TAGACAAGTC CGCATGCAAG CACTCACTTA CCTGCAAAGG
    GCACTTCTGG TCCATGATCT ACAAACACTG GATGCGGTGG AGTGGGAGTC CTGCTTCAAT
    AAGGTCCTGT TTCCTCTGCT GACCAAACTT TTGGAAAATA TCAGTCCAGC TGATGTGGGT
    GGCATGGAGG AGACCAGGAT GAGGGCCTGC ACACTGCTTT CCAAGGTGTT CCTGCAGCAT
    CTCTCTCCTC TGCTCTCTCT GCCCACGTTT GCTGCCCTCT GGCTCACCAT TCTGGACTTC
    ATGGACAAGT ACATGCACGC TGGATCCAGC GACCTTCTGC TGGAGGCCAT CCCTGAATCC
    CTGAAAAACA TGCTGCTGGT AATGGACACA GCAGGGATCT TTCATAGCGC AGACTCCCGC
    ACAGGATACT CAGACCTGTG GGAAATCACC TGGGAGAGAA TAGTCTGTTT CCTGCCCCGT
    CTAAAGGAGG AGCTCTTCAA ACAGGCTGTC ATTCCAGAGC CTGTGTGTAA TGTTCCTGTG
    GAGCCCGTCC CGCCTCCTGT TGCTGTTCCT CCAGCCGCTC CCACTGTTCC AGCTCCATCA
    CCAGTGCCAG CAGCCCCTGC AGAGCCAAAT ATCCCCAGCA GACCCTCATC GCCTCAAGAC
    ATGCCGACAC CGAGTGCCAA CGGTGATCGA ACAGACAGAT CATCTCCAGT TCCTCCCTCA
    ACACCTCCGC TACCCACTCC AGCCAAGAAG AGTCCTCCAG AGTCCCCGCC ACCTCTCAGC
    CAGTCCCCGC TCATCCTCCA GCCCCTCGCC TCCCCTTTGC AGGTGGGCGT CCCCCCTATG
    ACCCTGCCAA TCATTCTGAA CCCCGCCCTC ATAGAGGCCA CCTCGCCCGT GCCCCTGCTG
    CCCCGCCCCT CCGCAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305383.1
    CDS156..5696
    Translation

    Target ORF information:

    RefSeq Version XM_009307108.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307108.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATATA CATCGTTCAA GGGGAGATTT CTGTTGTCGT TGCTGCAATC 
    AAGAGGAACT CTAGATGGAG TACCCATACA CCTTTGGATG AAGAACAGGA CACACTGCTC
    AACAGCTTCA GGCTTTTGAA AGAGAGACTC AATAACATCA AAGATCTTTC CGATGTGGAA
    CCCAATGTGT TTCTAAGACC TTTTCTGGAG GTGGTTCGCT CAGAAGACAC AACTGGACCA
    ATCACAGGCC TTGCTCTCAC ATCGGTCAAC AAGTTCTTGT CTTATGGACT TATAGATGCC
    AATCATGAAG GGGCAGCGGA GTGCATCGAG AACATGGCTG ATGCGGTCAC GCATGCAAGA
    TTCGTGGGCA CCGACCCTGC AAGTGATGAG GTGGTGCTGA TGAAAATCCT CCAGGTTTTG
    AGGACTTTGC TATTGACTCC TGTTGGAGCC CATCTCACCA ATGAGTCTGT CTGTGAGATC
    ATGCAGTCCT GTTTCCGTAT ATGCTTTGAA ATGAGATTAA GTGAACTGTT GAGAAAGTCA
    GCTGAGCACA CGCTGGTGGA CATGGTGCAG CTGCTCTTCT CCAGATTGCC ACAGTTCAAA
    GAGGAGGCCA AGAGTTATGT GGGCACTAAC ATGAAGAAGC TAAAGATGAG AGCCGGGGGA
    ATGAGCGAGT CGTCCAAGTG GAAGAAACAG AAGCGTTCTC CCCGTCCCCC TCGTCACATG
    GTGCGCAGTC CCTCAGGACA GATGGATCCA GCTCAGTCCA GCACTCTCAC TAACAACCTT
    TCAGGTGGAG TTCCCTTCAT AGAGCAGCAC AGTGGAGGTT CTTCTACATT TCCTGGTTCC
    GACAGTGCAG CTTCCTCCAT CTCCAGCCCC ACCACTGACA GTGGCTTAGA CACCGGCTCC
    AAAACCACCT CAAAGGAGGA TCTCACCGAC CTGGACCAGA ACAGCTCAAC AGCCACCACT
    CCCATCACAC CCTCCGCAGA GCCGGGCAGA CTGGAATCAC AGTCTGAGGG CAGAGGTGTG
    GAGCGAGCCC AGTCCGCCTC AGTGGAGTCC ATTCCTGAAG TCCTGGAAGA CCGAGACCTG
    CTGGCTGACC AGTCTGATTC AGCATCTGTC CACGATATGG ACTATGTGAA CCCTAGAGGA
    GTGCGCTTTA CACAGTCTAC TCAGAAAGAA GGTGCTGCTC TTATCCCGTA TGGTCTGCCA
    TGTTTGAGAG AGCTCTTCCG GTTCCTCATC TCTCTCACCA ACCCACACGA CCGCCATAAC
    TCCGATGTGA TGATGCACAT GGGCCTTCAG CTCTTAAACG TTGCCCTTGA AGCTGCACAC
    ATCGCCCCCT ACCAGTCATT GCTCGGTCTG GTCAAAGATG AACTCTGTAG GCACCTCATA
    CAGTTGTTAG GTGTAGACCG AATGAATCTG TACACCGCCT CTATACGTGT GTGCTTTCTT
    CTGTTTGAAA GCATGCGAGA GCATCTAAAG TTCCAACTTG AGATGTACCT GAAGAAGCTA
    ATGGACATCA TTACCTCAGA GAACCCGAAG ATGCCATATG AAATGAAGGA GATGGCCCTG
    GAGGCCATAG TACAGCTCTG GAGAATCCCC AGCTTTGTCA CCGAGCTCTA CATAAATTAC
    GACTGTGACT TTTACTGCTC CAACCTGTTT GAGGATCTCA CCAAATTGCT CTCTAAGAAT
    GCCTTTCCAG TGTCTGGGCA GCTTTACACA ACCCATCTCC TGTCACTTGA AGCCCTGCTG
    ACAGTGATTG ACAGCACCGA GGCTCACTGT CAGGCCAAAG TGCTCAACAG CGCTACACAG
    CAGGAACAGT CCGAATCCAC AACCGTAGGA GACAGTTCAG TCAGTACCAT CACAGATTCA
    CCCGCAGAAA CTGGCAAACC TCATCCCAGT TCTAATGGAC AGAACTCTGT GGTGTCTGAG
    ACGAGGGCTT CCTGTCCTCC CACCAGCGGT CATCTCATGG CAGAGAAGAT GAGATTAGGT
    AGACAGGACC AGGAGGAGAC GGACACAGGA GAGAAGAAAA TCTCGAAGAA GCCTCATCGT
    TTCTCCAGCT ATCTTCCAGA CTCTCAGGAA CTTCTGGAAA TCAAGAACAA GAAGAAGCTT
    CTGATCACGG GTACAGAACA GTTCAATCAG AAGCCAAAGA AAGGCATTCA GACCCTGCAG
    GAGAAAGGCC TGCTCAGCAG CCCCATGGAT AATAACGAAG TAGCCCAATG GCTGAGAGAA
    AACCCCCGTC TGGATAAGAA GATGATTGGA GAGTTTATCA GCGATCGCAG GAATACTGAC
    CTGCTGGACA GTTTTGTCAA CACGTTCACC TTCCAGGGTC TGCGCATAGA CGAGGCCCTC
    CGCTTGTATC TGGAGGCTTT CCGTCTTCCT GGCGAAGCGC CTGTCATTCA TCGACTTTTG
    GAAACCTTCA CTGACAACTG GCATAAAGTG AACGGTAATC CCTTCCAGAC AAATGACGCT
    GGCTTCGCCC TGGCTTACGC CGTGATCATG TTAAACACCG ATCAGCACAA CCATAACGTT
    CGAAAGCAGA ACATCCCCAT GACTTTGGAG CAATTCAAGA AGAATCTCAA AGGTGTGAAC
    GGAGGAAATG ACTTTGATCA AGATATGCTG GAAGACATCT ACAATGCCAT CAAGAACGAG
    GAGATTGTGA TGCCGGACGA GCAGACGGGG CTGGTGAAGG AGAACTACGT GTGGAGCGTT
    CTGCTGCACA GAGGAGCCTC CGCTGAAGGG ATGTTCCTGC ATGTGCCGGA CGGCAGTTAT
    GACCGTGACC TCTTCTCCAT GACCTGGGGA CCCACCATTG CCGCCCTCTC CTATGTTTTC
    GACAAGAGCC TGGATGATAC TATTATCGAG AAGGCTATTG CTGGCTTTAG GAAATGTGCC
    ATGATCTCTG CACACTACGG CTTCAGTGAC GTCTTTGATA ACTTGATCAT TTCACTTTGC
    AAGTTTACTA CACTCAGCTC TGAGTCAGTA GAGAACCTAC CCACCGTGTT TGGCAGTAAC
    AGGAAGGCTC AAGTCGCCGC TAAGACTGTC TTCAGCCTGG CGCATCGTCA TGGTGATATT
    TTACGAGACG GATGGAAAAA CATCATGGAC TCCATGTTGC AGCTTTTCAG GGCTGAGCTT
    TTACCCAAGA CCATGGTCGA GGTTGAGGAC TTTTTGGATC CTAATGAGAA GATTTCTTTA
    CAACGTGAAG AGACGCCATC AAACAGGGGT GAGTCTGCTG TTTTGAGTTT TGTCAGCTGG
    TTAACACTGA GCGAACAATC AGGGCTCAGA GGTCCCTCCA CTGAGAACCA GGAGGCCAAG
    CAAGCTGCAC TGCTCTGCAT AAAGCAATGT GATCCAGAAA AGCTGAACAC AGAAAGCAAG
    TTTCTGCAGC TGGAGTCTTT GCAGGAGCTC ATGAAGGCTC TCATCTCTGT GACCCCTGAT
    GAGGAAACAT ATGATGAAGA AGATGCTGCT TTTTGCTTGG AGATGCTGCT ACGTATCATT
    CTTGAAAACA GGGACAGAGT ATCGTGTGTT TGGCAAACGG TGCGGGATCA TCTGTATCAG
    TTGTGTGTCC ATGCTAATGA GAGCTGCTTC CTGGTGGAGA GAGCAGTGGT GGGATTGCTC
    AGGCTTGCCA TACGCTTGTT GCGCAGAGAG GACATCGGCT CACAGGTTCT CCTCTCCCTT
    CGCCTGCTGC TGATGATGAA ACCTCACGTC CTGTCTAGAG TCAGTCGGGA GGTGGCCTAC
    GGCTTGCATG AGCTGCTTAA AACTAACGCT GCCAACATCC ACAGCACTGA CGATTGGTAC
    ACTCTCTTTT CCCTCCTGGA GTGCATCGGC GCTGGGATCA AACCTCCGGC TGCTCTGCAG
    TTCGCCAACA CTAACCCAGA CAATGACACA GGCGCGCAGT CAGACAGTGA ACTCAGCTCA
    TATCATCAGA GTGAAGTTAG TCTTGACCGG GGATACACGT CCGATTCTGA GATTTACAAC
    GAGCATGGCA AATCCAGAAT ACCCCGTTCT GCTACTGATG TGGATGTAGG CTGGCTAGTG
    GTGGGTAAAG ATGACCTGGA CGGAGCTAAG GCAGCGGTGA CCACACCTTC AAAGCCCATG
    AACCATCCTC TCGTTAACCA GTACAGTTTA ACTCTCGGGC AGGACATGGG TCTGCATGAT
    ACCAAGTCCC TCATCAAGTG CGTGGATTCG CTTTCCTTCA TCGTACGCGA TGCTGCTCAC
    GTCACTCCAG AAAACTTTGA GCTTTGTGTT AAAGCCATTC GTGTGTTCGT GGAGGCCAGC
    CTTAATGGAG GCTACCGCAC CCACGAGAAG AAGAAGAGCC ACAAATACGA CTCCAAATCC
    AGCCGGCTCC GAAAGAAGCC ACGGGAGAAG GAAGGTGCAT CACGAAGAAC CAAAGCGTCC
    AGTCAGCGGC CCGCCCGTTC CCATAGCGAC GATGAGGAGG AGGAGGGCGT GCCGGCCAGT
    TACCACACGG TGTCTTTTCA GTTGTTAGAC CTGATGCACA CACTGCACAC GCGAGCAGCC
    GGCATCTACA GCTCCTGGGC GGAGGAACAG CGCCATCTAG AGGATGCAGG AAAGAAGATC
    GAGGCAGACT CTCAAACGCT GTGGACCAGC TGCTGGTGTC CGCTCCTTCA AGGTATGGCT
    TGGTTGTGCT GTGACGCACG TAGACAAGTC CGCATGCAAG CACTCACTTA CCTGCAAAGG
    GCACTTCTGG TCCATGATCT ACAAACACTG GATGCGGTGG AGTGGGAGTC CTGCTTCAAT
    AAGGTCCTGT TTCCTCTGCT GACCAAACTT TTGGAAAATA TCAGTCCAGC TGATGTGGGT
    GGCATGGAGG AGACCAGGAT GAGGGCCTGC ACACTGCTTT CCAAGGTGTT CCTGCAGCAT
    CTCTCTCCTC TGCTCTCTCT GCCCACGTTT GCTGCCCTCT GGCTCACCAT TCTGGACTTC
    ATGGACAAGT ACATGCACGC TGGATCCAGC GACCTTCTGC TGGAGGCCAT CCCTGAATCC
    CTGAAAAACA TGCTGCTGGT AATGGACACA GCAGGGATCT TTCATAGCGC AGACTCCCGC
    ACAGGATACT CAGACCTGTG GGAAATCACC TGGGAGAGAA TAGTCTGTTT CCTGCCCCGT
    CTAAAGGAGG AGCTCTTCAA ACAGGCTGTC ATTCCAGAGC CTGTGTGTAA TGTTCCTGTG
    GAGCCCGTCC CGCCTCCTGT TGCTGTTCCT CCAGCCGCTC CCACTGTTCC AGCTCCATCA
    CCAGTGCCAG CAGCCCCTGC AGAGCCAAAT ATCCCCAGCA GACCCTCATC GCCTCAAGAC
    ATGCCGACAC CGAGTGCCAA CGGTGATCGA ACAGACAGAT CATCTCCAGT TCCTCCCTCA
    ACACCTCCGC TACCCACTCC AGCCAAGAAG AGTCCTCCAG AGTCCCCGCC ACCTCTCAGC
    CAGTCCCCGC TCATCCTCCA GCCCCTCGCC TCCCCTTTGC AGGTGGGCGT CCCCCCTATG
    ACCCTGCCAA TCATTCTGAA CCCCGCCCTC ATAGAGGCCA CCTCGCCCGT GCCCCTGCTG
    CCCCGCCCCT CCGCAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.