fstl5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fstl5
Entrez Gene ID	566192
Full Name	follistatin-like 5
Synonyms	drMahya-1,zgc:136225
General protein information	Preferred Names follistatin-like 5 Names follistatin-related protein 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031842.2	NP_001027012.2	follistatin-related protein 5 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FSTL5	XP_004940968.1
Homo sapiens (human)	FSTL5	NP_001121899.1
	FSTL5	XP_001097841.1
Pan troglodytes (chimpanzee)	FSTL5	XP_517513.3
Canis lupus familiaris (dog)	FSTL5	XP_005629445.1
Mus musculus (house mouse)	Fstl5	NP_001240648.1
Danio rerio (zebrafish)	fstl5	NP_001027012.1
Xenopus tropicalis (tropical clawed frog)	fstl5	XP_002934788.2
Bos taurus (cattle)	FSTL5	NP_001193792.1
Rattus norvegicus (Norway rat)	Fstl5	NP_001258124.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Conservation of novel Mahya genes shows the existence of neural functions common between Hymenoptera and Deuterostome.
Tsuchimoto M, Yasuo S, Funada M, Aoki M, Sasagawa H, Yoshimura T, Tadauchi O, Cameron SA, Kitagawa Y, Kadowaki T
Development genes and evolution215(11)564-74(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fstl5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fstl5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa44816	NM_001031842.2 Latest version!	Danio rerio follistatin-like 5 (fstl5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fstl5 ( NM_001031842.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001027012.2 Danio rerio follistatin-like 5 (fstl5), mRNA.

Price & Availability↑

CloneID	ODa44816
Clone ID Related Accession (Same CDS sequence)	NM_001031842.2
Accession Version	NM_001031842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	follistatin-related protein 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX548246.17, CABZ01086187.1, CU856352.17, CU896660.5, CU861652.12 and CT956065.9. On Jun 16, 2015 this sequence version replaced NM_001031842.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB231586.1, GFIL01010845.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACTTGTG GTTGGACACA CAGGAGTGTA ATGTGCAGGT GTTGGGCAGT TGTTCTGCTT 
TGGGCCGCAG CTCTGATGGC AGAGGCTCGT CCTGGTAAAG AAGGATTCAC CCCGCCGGCA 
TATCGACCAC TCGTCCGCTT CCGTCACAAG GATGGGATCT CAGAGAGTTT ACGGATGAAA 
GGGTTTCTTG GTCAGACTGG TTTTCCCGGG CCCTGTGAAC ATAAGTACTG TGGTTTGGGA 
AAACATTGTG TGGTGGACAG AGAAACAGGT GAAGGAGAGT GTCAATGTCT GGAGCGCTGC 
AAACCACATT ACAAACCTGT GTGCGGATCT GACGGGAAAC TGTACCAGAA CCACTGTGAG 
CTCCACAGAG CCTCCTGTCT GGCCCATCAG AGAATAACCA TCATGCACAG CGACGAATGC 
TTCTACAAAG ATGATAACTG CCGTCTGGGA GATTATAAAA AATTGAAGAG CAAGATGTTG 
GACATACATG CTCAGAGGTA CTTGACCGCC GCTAACCATG GCAGCCACGA GAAGGATATG 
GCCGCTAGGA AGCATCTGGT GGACATGATG TTTAAGCGAT TTGACGCAGA CGGCAATGGA 
AAAGTTGACA GCAGCGAGCT TTCACAGGTC ATCAAGCAGG AAGGTTTGAG CAGAACAGTG 
TCCGAATGCA CTCTCTTCGA CCTCTTTAAA TACAATGACA TCAACGATGA CGAACACCTT 
GCTAAAGAAG AGTTTTATGC AGCTTTTGAG GTGTACCAGC TCAGCCTTCC AGAAGAGCAG 
AAGCTGAGCG TCACCACGGT AACAGTGGGT CAGAGCGCTG TCCTCACGTG TGCCATCATG 
GGGGACCAGC GGCCTCCCAT CATCTGGAGA CGCAACGGCC ACGATCTCAA CATGATGGAG 
CTGGAGGACA TCAACGATTT CGGAGATGAT GGATCACTGT ACATTACCAA GGTGACCACT 
ACACATATGG GCAACTACAC CTGCCATGCT GACGGATACG AGCAGCTCTT CCAGACACAC 
ACCCTGCAGG TTAATGTCCC TCCTGTTATC CGAGTGTACC CAGAGAGCCA GGCACGTGAG 
CCGGGCGTCA CAGCCAGTCT GCGGTGCTAT GCAGAGGGCA TCCCTGACCC ACAGCTGTCC 
TGGCTGAAGA ACGGCATGGA CATCACCACT AAACTCTCCA AACAGCTCAC ACTTCAAGCA 
AACGGGAGCG AGGTTCACAT CAGTAACGTC CACTTTGAGG ACACCGGTGC TTATACCTGC 
ATCGCCCGCA ATGAGGCCGG CGTGGATGAG GACATCTCTT CTTTATTCGT GGAGGACTCG 
GCTCGCAAAA CATTGGCAAA CATCCTGTGG AGGGAGGAAG GTCTGGGGAT TGGAAACATG 
TTCTATGTGT TTTATGAGGA TGGTATTAAA GTTATCCAGC CAGTGGCCTG TGAGATTCAG 
AGGCACATCA AGCCCAGCGA GAAGCTTCTT GGCCTGCAGG AGGAGGTTTG TCCGCTTGTG 
GAAGGAGAGA TGGAGCAGAA ATGCTTGTGG ACGTCTGCCG TCAACGTGAA GGACAAGTTC 
ATTTACGCAA CACAGCCTTT GCTGAACCGA CTGCTCATTG TGGACATCCA ATCTCAGAAG 
GCCGTTCAGA CTGTGAGCAC AGACCGGCTT CCAGTGAAGC TCCACTATGA TAAATCTCAT 
GACCAGGTGT GGGTGTTGAG CTGGGGAGAT CTGGAGAAAA ACTTCCCAAC GCTGCAGGTG 
ATCAGCCAGG CCAGCGGCAG CACATCTCAC CACACCATCC ACACACAGCC GGTCGGCCAT 
CGCTTCGACA GGGTGGAGGA TTTCTTCATT CCTCCCATCA GCCTCACCAT CAACCACATC 
AGGTTTGGTA TCGTCCTCCA TAAGAATGAA CCAGCTCTTC ACAAGATCGA CTTGGAAACC 
ACGTCATATG TTAAAAACAT CAGCCTGCAG CAGTACGACT GTATCCCACA ATCCCTGGCT 
TACACACACC TGGGCGGATA TTATCTGGTC AACTGTCGAC CGGACTCAAC AGGTGCGGCG 
CGGCCACAAT TAATCATCGA CAGCATAACA GACAACGTCA TGGGCCCTAA TGGCGACGTG 
AGCGGCACAC CTTATGTCTC TCCTGATGGC CACTATTTAG TGAGTGTGGA CGACCGTGAC 
GGGCTAATGC GCTTACAGCA TGTGTCCGTA CGGGGAGAGA TCGGCCAACC CTTTGACATT 
CACACCAACC TGCACCTGTC CGATCTGGCC TTCATGCCCT CGTTCACTGA GGCCAACCAG 
TATAATGTGT TCGGAAGCTC CGGCAGGCAG ACGGATGCTC TTTTTGTGGA GCTCAGTTCT 
GGCAGTGTCA AAATGATCAA GAGCTTGAAG CAGCCCACGG CGTCGGCGCA GTGGCCCTGG 
AACCGTCAGA ACCGAGTTAT GACCGGAAGC GGGCTGTTCG GACAGTATCT CATGACCCCG 
TCACAAAGCT CCCTGTTTAT ACTGGATGGG CGTCTGGATA AACTCAACTG TGAGATCACC 
GACGTTCCCT TGGGAAATAC TGTGGTGTGG GTGGGAGAGG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027012.2
CDS	330..2894
Translation	MTCGWTHRSVMCRCWAVVLLWAAALMAEARPGKEGFTPPAYRPLVRFRHKDGISESLRMKGFLGQTGFPGPCEHKYCGLGKHCVVDRETGEGECQCLERCKPHYKPVCGSDGKLYQNHCELHRASCLAHQRITIMHSDECFYKDDNCRLGDYKKLKSKMLDIHAQRYLTAANHGSHEKDMAARKHLVDMMFKRFDADGNGKVDSSELSQVIKQEGLSRTVSECTLFDLFKYNDINDDEHLAKEEFYAAFEVYQLSLPEEQKLSVTTVTVGQSAVLTCAIMGDQRPPIIWRRNGHDLNMMELEDINDFGDDGSLYITKVTTTHMGNYTCHADGYEQLFQTHTLQVNVPPVIRVYPESQAREPGVTASLRCYAEGIPDPQLSWLKNGMDITTKLSKQLTLQANGSEVHISNVHFEDTGAYTCIARNEAGVDEDISSLFVEDSARKTLANILWREEGLGIGNMFYVFYEDGIKVIQPVACEIQRHIKPSEKLLGLQEEVCPLVEGEMEQKCLWTSAVNVKDKFIYATQPLLNRLLIVDIQSQKAVQTVSTDRLPVKLHYDKSHDQVWVLSWGDLEKNFPTLQVISQASGSTSHHTIHTQPVGHRFDRVEDFFIPPISLTINHIRFGIVLHKNEPALHKIDLETTSYVKNISLQQYDCIPQSLAYTHLGGYYLVNCRPDSTGAARPQLIIDSITDNVMGPNGDVSGTPYVSPDGHYLVSVDDRDGLMRLQHVSVRGEIGQPFDIHTNLHLSDLAFMPSFTEANQYNVFGSSGRQTDALFVELSSGSVKMIKSLKQPTASAQWPWNRQNRVMTGSGLFGQYLMTPSQSSLFILDGRLDKLNCEITDVPLGNTVVWVGEA

Target ORF information:

RefSeq Version	NM_001031842.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio follistatin-like 5 (fstl5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031842.2

ORF Insert Sequence:

ATGACTTGTG GTTGGACACA CAGGAGTGTA ATGTGCAGGT GTTGGGCAGT TGTTCTGCTT 
TGGGCCGCAG CTCTGATGGC AGAGGCTCGT CCTGGTAAAG AAGGATTCAC CCCGCCGGCA 
TATCGACCAC TCGTCCGCTT CCGTCACAAG GATGGGATCT CAGAGAGTTT ACGGATGAAA 
GGGTTTCTTG GTCAGACTGG TTTTCCCGGG CCCTGTGAAC ATAAGTACTG TGGTTTGGGA 
AAACATTGTG TGGTGGACAG AGAAACAGGT GAAGGAGAGT GTCAATGTCT GGAGCGCTGC 
AAACCACATT ACAAACCTGT GTGCGGATCT GACGGGAAAC TGTACCAGAA CCACTGTGAG 
CTCCACAGAG CCTCCTGTCT GGCCCATCAG AGAATAACCA TCATGCACAG CGACGAATGC 
TTCTACAAAG ATGATAACTG CCGTCTGGGA GATTATAAAA AATTGAAGAG CAAGATGTTG 
GACATACATG CTCAGAGGTA CTTGACCGCC GCTAACCATG GCAGCCACGA GAAGGATATG 
GCCGCTAGGA AGCATCTGGT GGACATGATG TTTAAGCGAT TTGACGCAGA CGGCAATGGA 
AAAGTTGACA GCAGCGAGCT TTCACAGGTC ATCAAGCAGG AAGGTTTGAG CAGAACAGTG 
TCCGAATGCA CTCTCTTCGA CCTCTTTAAA TACAATGACA TCAACGATGA CGAACACCTT 
GCTAAAGAAG AGTTTTATGC AGCTTTTGAG GTGTACCAGC TCAGCCTTCC AGAAGAGCAG 
AAGCTGAGCG TCACCACGGT AACAGTGGGT CAGAGCGCTG TCCTCACGTG TGCCATCATG 
GGGGACCAGC GGCCTCCCAT CATCTGGAGA CGCAACGGCC ACGATCTCAA CATGATGGAG 
CTGGAGGACA TCAACGATTT CGGAGATGAT GGATCACTGT ACATTACCAA GGTGACCACT 
ACACATATGG GCAACTACAC CTGCCATGCT GACGGATACG AGCAGCTCTT CCAGACACAC 
ACCCTGCAGG TTAATGTCCC TCCTGTTATC CGAGTGTACC CAGAGAGCCA GGCACGTGAG 
CCGGGCGTCA CAGCCAGTCT GCGGTGCTAT GCAGAGGGCA TCCCTGACCC ACAGCTGTCC 
TGGCTGAAGA ACGGCATGGA CATCACCACT AAACTCTCCA AACAGCTCAC ACTTCAAGCA 
AACGGGAGCG AGGTTCACAT CAGTAACGTC CACTTTGAGG ACACCGGTGC TTATACCTGC 
ATCGCCCGCA ATGAGGCCGG CGTGGATGAG GACATCTCTT CTTTATTCGT GGAGGACTCG 
GCTCGCAAAA CATTGGCAAA CATCCTGTGG AGGGAGGAAG GTCTGGGGAT TGGAAACATG 
TTCTATGTGT TTTATGAGGA TGGTATTAAA GTTATCCAGC CAGTGGCCTG TGAGATTCAG 
AGGCACATCA AGCCCAGCGA GAAGCTTCTT GGCCTGCAGG AGGAGGTTTG TCCGCTTGTG 
GAAGGAGAGA TGGAGCAGAA ATGCTTGTGG ACGTCTGCCG TCAACGTGAA GGACAAGTTC 
ATTTACGCAA CACAGCCTTT GCTGAACCGA CTGCTCATTG TGGACATCCA ATCTCAGAAG 
GCCGTTCAGA CTGTGAGCAC AGACCGGCTT CCAGTGAAGC TCCACTATGA TAAATCTCAT 
GACCAGGTGT GGGTGTTGAG CTGGGGAGAT CTGGAGAAAA ACTTCCCAAC GCTGCAGGTG 
ATCAGCCAGG CCAGCGGCAG CACATCTCAC CACACCATCC ACACACAGCC GGTCGGCCAT 
CGCTTCGACA GGGTGGAGGA TTTCTTCATT CCTCCCATCA GCCTCACCAT CAACCACATC 
AGGTTTGGTA TCGTCCTCCA TAAGAATGAA CCAGCTCTTC ACAAGATCGA CTTGGAAACC 
ACGTCATATG TTAAAAACAT CAGCCTGCAG CAGTACGACT GTATCCCACA ATCCCTGGCT 
TACACACACC TGGGCGGATA TTATCTGGTC AACTGTCGAC CGGACTCAAC AGGTGCGGCG 
CGGCCACAAT TAATCATCGA CAGCATAACA GACAACGTCA TGGGCCCTAA TGGCGACGTG 
AGCGGCACAC CTTATGTCTC TCCTGATGGC CACTATTTAG TGAGTGTGGA CGACCGTGAC 
GGGCTAATGC GCTTACAGCA TGTGTCCGTA CGGGGAGAGA TCGGCCAACC CTTTGACATT 
CACACCAACC TGCACCTGTC CGATCTGGCC TTCATGCCCT CGTTCACTGA GGCCAACCAG 
TATAATGTGT TCGGAAGCTC CGGCAGGCAG ACGGATGCTC TTTTTGTGGA GCTCAGTTCT 
GGCAGTGTCA AAATGATCAA GAGCTTGAAG CAGCCCACGG CGTCGGCGCA GTGGCCCTGG 
AACCGTCAGA ACCGAGTTAT GACCGGAAGC GGGCTGTTCG GACAGTATCT CATGACCCCG 
TCACAAAGCT CCCTGTTTAT ACTGGATGGG CGTCTGGATA AACTCAACTG TGAGATCACC 
GACGTTCCCT TGGGAAATAC TGTGGTGTGG GTGGGAGAGG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Conservation of novel Mahya genes shows the existence of neural functions common between Hymenoptera and Deuterostome.
Development genes and evolution215(11)564-74(2005 Nov)
Tsuchimoto M,Yasuo S,Funada M,Aoki M,Sasagawa H,Yoshimura T,Tadauchi O,Cameron SA,Kitagawa Y,Kadowaki T

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fstl5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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