ATXN1B cDNA ORF clone, Danio rerio(Zebrafish)

The following ATXN1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATXN1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa21497 XM_689102.6
Latest version!
Danio rerio ataxin 1b (atxn1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa21497 XM_017351500.1
Latest version!
Danio rerio ataxin 1b (atxn1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa21497 XM_005158159.4
Latest version!
Danio rerio ataxin 1b (atxn1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
ODa21497 XM_689102.5 Danio rerio ataxin 1b (atxn1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa21497 XM_005158159.3 Danio rerio ataxin 1b (atxn1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa21497
    Clone ID Related Accession (Same CDS sequence) XM_005158159.4 , XM_689102.5 , XM_017351500.1 , XM_689102.6 , XM_005158159.3
    Accession Version XM_005158159.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ataxin-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158159.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158216.1
    CDS550..3033
    Translation

    Target ORF information:

    RefSeq Version XM_005158159.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ataxin 1b (atxn1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158159.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21497
    Clone ID Related Accession (Same CDS sequence) XM_005158159.4 , XM_689102.5 , XM_017351500.1 , XM_689102.6 , XM_005158159.3
    Accession Version XM_689102.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ataxin-1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689102.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_694194.1
    CDS971..3454
    Translation

    Target ORF information:

    RefSeq Version XM_689102.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ataxin 1b (atxn1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_689102.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21497
    Clone ID Related Accession (Same CDS sequence) XM_005158159.4 , XM_689102.5 , XM_017351500.1 , XM_689102.6 , XM_005158159.3
    Accession Version XM_017351500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ataxin-1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206989.1
    CDS347..2830
    Translation

    Target ORF information:

    RefSeq Version XM_017351500.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ataxin 1b (atxn1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21497
    Clone ID Related Accession (Same CDS sequence) XM_005158159.4 , XM_689102.5 , XM_017351500.1 , XM_689102.6 , XM_005158159.3
    Accession Version XM_689102.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ataxin-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689102.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_694194.1
    CDS1336..3819
    Translation

    Target ORF information:

    RefSeq Version XM_689102.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ataxin 1b (atxn1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_689102.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21497
    Clone ID Related Accession (Same CDS sequence) XM_005158159.4 , XM_689102.5 , XM_017351500.1 , XM_689102.6 , XM_005158159.3
    Accession Version XM_005158159.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ataxin-1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158159.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158216.1
    CDS667..3150
    Translation

    Target ORF information:

    RefSeq Version XM_005158159.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ataxin 1b (atxn1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158159.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGTCTA ACCAGGAGCG CTGTAATGAG TGCCTTCCGC CAAAGAAGCG GGAGATTTTA 
    GCACTGGAAG AGAAGCAGGT GCTGGTGTCA GCTGCTGTTA GTGAGAGCCA GAGTGGAGAA
    AATTTGGCTT GGCTAGCCAG TGTTGCTACC ATGGCTAGCA TGGCCCACAT CCCCAACAAT
    GCAGGCCCAT CACATAGCAA CAGTTCTGAG CCTGACAGCC CCCCACCATC AAATAAGGCT
    CTCACTGTGG TGGCAGAGTA CCCTCCAACT ACTACATCTG CTGGCTTTTC ATTCTCTTCC
    AATAGTTCCA CCTACAGAGG AGTCTTGTCG GGGCCTACCG TACTAACAGC AAGTCCTGCG
    GTTCTCTCCA CAAGCCTTCC TCAAACCATA GGTTCCGTCC AATACACACA ACTTCCCCCC
    AATCTCCAGC TCATAGGGCC CTATACAAGT TATATCTCTT CACATATTGT CCCATCCACA
    TCCAGTCCAA CACAACCACA GAGAACACCA CAAGAGATCT TGGCCACAAC AGCTGTCATC
    TCTCAAGCAT CCAGCCTTGA TTCACAGAGT CATGTGATTT CTTCTATACC AAATGTTTCC
    CACAGTCAAT GCATCCAGGT CGAGAGTGCT CCTTTGGGTT TGACAGTTTC TTCTCCTTCT
    ACACAACTGC CCCTTCAAAT CCATCCACAC TCAGCTGTCC TTGCTCCTCA TGCCCTGGCT
    CTCACCCCCT CTCAAGTGGT ACTACACTAC ACCGATGGCT TCATTGCAAA GCAGCCAGAC
    TCCCATCGCA GAGAGGTGCT GAATGGTGCA CTAGGGGATG TTACAGTAGT CAAGCACAAT
    ACTACAATGG CCAAAGGGAT CTCTAGCCAG CCAGATGTTG AACAGAACCA CAAGCAAAAC
    CATAACCTGG GTATCCAGAC GCAAGCTCAG GTTCTGCTGC CTGCAGACTA CACCACTAAT
    GACAGAGCAG GTCTGCAGAC TTCGCTGATG CTGGTATCCA GTAGCCACCT TGCAGCAAAC
    AGCAACTCGG ACATCCAAAG TATCTTGGGT AAAGACCACT CATCTTTGCA CCTTGCTGAG
    AGAGGAGGGA TCTGCCTAGG CAAGCCAATG TCCAGAGTGT CCTCCCTTAC TTCTTCAGAC
    AGCCAAGTTT CTCCTGCCTC AACAATTTCC CCCCACACAC TCATCCAGGC TCCTCACAAT
    GCTCCTCAAC AGGAACTCTC CACCAGCCTT TACTCTGCTA CACAGCTCCC AATTATTGGC
    TACATCACCA GTGCTTCTGG TGGACCTCAG CAAGCAGTAA CTGGTTATCA GAGCAACCTG
    CCACAGCATG TGGTAATCTC AGGCAACCCC TCCTTGCTCA TTCCGGTGAG TGCCACTAAC
    ATCAATCCGT CTACTGCTGA GCCGGAGGTC ACACGCTGTT CTGTCATCCC CAGTTTAGCT
    AATGCATCAA CAGCTCTGCC CCAGACCTTT ATTAATACAT CCCCACCTGC AGCAGTAGCT
    TCAGTTCTAC CCAATGGTAA AGACATCACA GGGTCTGATG GAGGTCTTGT CCCCTGTGCT
    GTGCCAAGTC CTACCCAAAT ACAGCTGCCT GTTCTTTCAG GCAGTGTAGT GAGATCTCCA
    GTCCCAGCTA CACCTCCAAT TTCCACAAGC CCTCACAGCT CACCATCATC ACCAACAGCA
    GGACTTCCTC CATTCTTCAT GAAGGGCTCT ATTATCCAGT TGGCAGATGG CGAATTGAAG
    CGTGTGGAGG ATCTGCGGAC AGAGGACTTT GTGCAAAGTG CTGAGGTTAG TGGAGAACTG
    AAGATTGACT CCAGCACTGT AGAGCGCATA GAGGGCAGCG GCACTCCTAA TGCTGTCATC
    ATACAGTTCT CAGTGGGAGA GAACAAAGCC CAGGTGTGTG TTGAGGTTCT GGTGGAATAC
    CCTTTCTTTG TGTTTGGTCA AGGTTGGTCA TCCTGCTGCC CTGATCGCAC CACCCAGTTA
    CTAGCGCTAT CCTGTGCCAA ACTCTCTGTG GGCGATGTCT GTATCTCTCT CACTCTTAAA
    AGTCTAAAAA ATAGCATTCA AAAGAAGGAT CATTTTTCAG ACTCTGTGAT GAAACACAGC
    CTTTTAAATG CAACCAAAAA CAATGGGCCT CTTATGGAAA GACCAACCCA AGTGGATGAT
    CTCAAGAAAG GACACAAAAT TCCATCAGAA GTGGAAAATA GTATTGTAGA AAAACTTATT
    GAGACTTCCA ACACAAAAAT TGTGTTGTCC CTTTCTGAGA ATGGGGGCTC AAATCCTCAG
    ACACTAGTTA ATCTTCAGAA CCAAGCTGAA AAAACATATA CATCCACTGC AGAGAGACCC
    ATTAGTCGCA AGAGGAGATG GTCTGCCCCA GAGAAAGCAG ACATGCAGAA GTCGCGAGAG
    GACCCTCAGA TTTTACCCAA ATTTTCCTTC ATCCCACATC AGCTGAAGGT CAGCATTGAA
    GGCCGTTCCA GCACAGGTTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.