nrxn1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nrxn1a
Entrez Gene ID	565531
Full Name	neurexin 1a
General protein information	Preferred Names neurexin 1a Names neurexin 1a neurexin Ia-alpha neurexin Ia-beta neurexin-1a-alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are synaptic transmembrane receptors that bind endogenous ligands that include neuroligins, dystroglycan, and neurexophilins. Neurexin complexes are required for efficient neurotransmission and are involved in synaptogenesis. In vertebrates, alternate promoter usage results in multiple isoform classes, of which the alpha and beta classes are the best characterized. In humans, allelic variants in this gene are associated with Pitt-Hopkins-like syndrome-2, while deletions have been associated with autism and schizophrenia. In zebrafish, morpholino knockdown of an alpha isoform results in defects in balance and reduced locomotor activity. Knockdown of a beta isoform results in defects in sprouting angiogenesis and vascular remodeling. Alternative splicing and promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2016].

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306645.2	XP_009304920.1	neurexin 1a isoform X5
XM_009306649.2	XP_009304924.1	neurexin 1a isoform X9
XM_005156209.3	XP_005156266.1	neurexin 1a isoform X10
XM_009306651.2	XP_009304926.1	neurexin 1a isoform X12
XM_021480136.1	XP_021335811.1	neurexin 1a isoform X11
NM_001346923.1	NP_001333852.1	neurexin 1a isoform 2 precursor
XM_009306644.2	XP_009304919.1	neurexin 1a isoform X4
XM_009306646.2	XP_009304921.1	neurexin 1a isoform X6
XM_009306650.2	XP_009304925.1	neurexin 1a isoform X11
XM_009306652.2	XP_009304927.1	neurexin 1a isoform X13
XM_009306655.2	XP_009304930.1	neurexin 1a isoform X16
XM_009306643.3	XP_009304918.1	neurexin 1a isoform X3
XM_021480137.1	XP_021335812.1	neurexin 1a isoform X12
XM_021480142.1	XP_021335817.1	neurexin 1a isoform X21
XM_009306643.2	XP_009304918.1	neurexin 1a isoform X3
XM_009306654.2	XP_009304929.1	neurexin 1a isoform X15
XM_009306642.3	XP_009304917.1	neurexin 1a isoform X2
XM_009306646.3	XP_009304921.1	neurexin 1a isoform X6
XM_009306651.3	XP_009304926.1	neurexin 1a isoform X13
XM_021480139.1	XP_021335814.1	neurexin 1a isoform X15
XM_009306654.3	XP_009304929.1	neurexin 1a isoform X19
XM_009306641.2	XP_009304916.1	neurexin 1a isoform X1
XM_009306642.2	XP_009304917.1	neurexin 1a isoform X2
XM_009306656.2	XP_009304931.1	neurexin 1a isoform X19
XM_009306641.3	XP_009304916.1	neurexin 1a isoform X1
XM_009306644.3	XP_009304919.1	neurexin 1a isoform X4
XM_009306645.3	XP_009304920.1	neurexin 1a isoform X5
XM_009306648.3	XP_009304923.1	neurexin 1a isoform X7
XM_021480140.1	XP_021335815.1	neurexin 1a isoform X16
XM_009306653.3	XP_009304928.1	neurexin 1a isoform X18
XM_021480145.1	XP_021335820.1	neurexin 1a isoform X24
XM_021480146.1	XP_021335821.1	neurexin 1a isoform X25
XM_009306656.3	XP_009304931.1	neurexin 1a isoform X26
NM_001080021.2	NP_001073490.1	neurexin 1a isoform 1 precursor
XM_009306648.2	XP_009304923.1	neurexin 1a isoform X8
XM_009306653.2	XP_009304928.1	neurexin 1a isoform X14
NM_001346924.1	NP_001333853.1	neurexin 1a isoform 3 precursor
XM_009306649.3	XP_009304924.1	neurexin 1a isoform X8
XM_005156209.4	XP_005156266.1	neurexin 1a isoform X9
XM_009306650.3	XP_009304925.1	neurexin 1a isoform X10
XM_021480138.1	XP_021335813.1	neurexin 1a isoform X14
XM_009306652.3	XP_009304927.1	neurexin 1a isoform X17
XM_021480141.1	XP_021335816.1	neurexin 1a isoform X20
XM_021480143.1	XP_021335818.1	neurexin 1a isoform X22
XM_021480144.1	XP_021335819.1	neurexin 1a isoform X23

Pathways from BioSystems

Homologies

Homo sapiens (human)	NRXN1	NP_001129131.1
Caenorhabditis elegans (roundworm)	nrx-1	NP_001122868.2
Xenopus tropicalis (tropical clawed frog)	nrxn1	XP_002935428.2
Pan troglodytes (chimpanzee)	NRXN1	XP_003309056.1
Rattus norvegicus (Norway rat)	Nrxn1	NP_068535.2
	NRXN1	XP_002799265.1
Mus musculus (house mouse)	Nrxn1	NP_064648.3
Canis lupus familiaris (dog)	NRXN1	XP_005626154.1
Bos taurus (cattle)	NRXN1	NP_776829.1
Gallus gallus (chicken)	NRXN1	NP_001185904.1
Danio rerio (zebrafish)	nrxn1a	NP_001073490.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of neurexin2a in lead-induced locomotor defect in developing zebrafish.
Tu H, Peng T, Liu J, Chen X, Fan C, Huang Z, Zhang Y, Zou F, Meng X
Aquatic toxicology (Amsterdam, Netherlands)194167-175(2018 Jan)

Maternal and embryonic exposure to the water soluble fraction of crude oil or lead induces behavioral abnormalities in zebrafish (Danio rerio), and the mechanisms involved.
Wang Y, Shen C, Wang C, Zhou Y, Gao D, Zuo Z
Chemosphere1917-16(2018 Jan)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Demonstration of the Coexistence of Duplicated LH Receptors in Teleosts, and Their Origin in Ancestral Actinopterygians.
Maugars G, Dufour S
PloS one10(8)e0135184(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Coinjection of low doses of morpholinos for beta-neurexin 1a and neuroligin 1 together or in combination with morpholinos targeting the -heparin--binding isoforms of vascular endothelial growth factor A recapitulates the observed abnormalities
Title: The synaptic proteins ?-neurexin and neuroligin synergize with extracellular matrix-binding vascular endothelial growth factor a during zebrafish vascular development.

The following nrxn1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrxn1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa98468	NM_001346923.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa98475	NM_001346924.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa148209	XM_021480136.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148210	XM_021480137.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148211	XM_021480138.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148212	XM_021480139.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148213	XM_021480140.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148214	XM_021480141.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148215	XM_021480142.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148216	XM_021480143.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148217	XM_021480144.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148218	XM_021480145.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148219	XM_021480146.1 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa44667	XM_009306655.2 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44658	NM_001080021.2 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44652	XM_009306641.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44653	XM_009306642.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44654	XM_009306643.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44655	XM_009306644.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44656	XM_009306645.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44657	XM_009306646.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44659	XM_009306648.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44660	XM_009306649.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44661	XM_005156209.4 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44662	XM_009306650.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44663	XM_009306651.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44664	XM_009306652.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44665	XM_009306653.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44666	XM_009306654.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44668	XM_009306656.3 Latest version!	Danio rerio neurexin 1a (nrxn1a), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa44654	XM_009306643.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44657	XM_009306646.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44663	XM_009306651.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44666	XM_009306654.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44653	XM_009306642.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44652	XM_009306641.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44655	XM_009306644.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44656	XM_009306645.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44668	XM_009306656.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44659	XM_009306648.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44665	XM_009306653.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44660	XM_009306649.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44661	XM_005156209.3	Danio rerio neurexin 1a (nrxn1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44662	XM_009306650.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44664	XM_009306652.2	Danio rerio neurexin 1a (nrxn1a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrxn1a ( NM_001346923.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001333852.1 Danio rerio neurexin 1a (nrxn1a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa98468
Clone ID Related Accession (Same CDS sequence)	NM_001346923.1
Accession Version	NM_001346923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX255907.8 and CR933760.7. On Nov 9, 2016 this sequence version replaced XM_017358469.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ641425.1, AB214179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCGTC TGTGGCCCGG CGGGGCTCCT GGTGGTCTTG CTTCCATCCT GCTCCGCATC 
AGCCTGCGCC TAGCTCTCTG GCTGCCACCG CTGACTTTGG GGTCAGCTCT GGCAGAGGGG 
CCTGGGGAGC TGTACGTCCC TCAACATATG CCTCATCACC TCGTGCCTGC CGCCAGATCC 
CACGCCCCCC TGCGGGCAGG ACATGCGGGA ACCACATACA TATTCGGACG TGATGGAGGG 
CTTATTGTGT ACACATGGCC ACCCAACGAC AGACCGAGCA CACGAGCTGA CCGACTGGCT 
GTCGGTTTTA GTACCCAGCA GAAGGATGCA GTGCTGGTTA GGGTGGACAG CTCTTCTGGC 
CTGGGAGACT ACCTTCAGCT TCAGATTGAA AGAGGCAATA TCAAAGTGGT GTTCAATGTG 
GGCACCGATG ACATAAACAT TGAAGAAACT TCAAAGTTTG TAAATGATGG AAAGTACCAC 
ATAGTTCGCT TTACGAGAAG TGGTGGTAAC GCCACCTTGC AGGTGGATGA TCTGCCAGTC 
ATTGAGCGCT ACCCATCAGG CAACATTGAT AACGAACGCC TGGCGATTGC TAGACAGCGA 
ATCCCATATC GGCTTGGTCG AGTAGTTGAC GATTGGCTAC TCGACAAAGG TCGGCAGCTC 
ACCATCTTCA ACAGTCAGAC CACCATAAAG ATTGGAGGCT GGGAGAAGGG CAGCCGGCCC 
TTCCAGGGTC AGCTCTCAGG GCTTTACTAC AATGGGCTAA AAGTACTCAA CATGGCCGCT 
GAGGGTGACC CTAATGTGCG TGTGGAGGGT AGTGCCCGGC TGGTAGGAGA CATGCCCTCC 
TCTTCTATCA CGCCACAGTC CAGCGTCTCT GCTGCGGGAA ATCGTTCAGA AACGTCACCC 
TCCATCACTG ACATCACCAC TACAACGGCG TCCAACCGCC AGGGCAAGCA GACCACAACT 
CCACAGGACG ATCTTCTCGT AGCGTCTGCA GAATGCCCCA GCGATGATGA GGACATTGAC 
CCTTGCGACC CAAGTTCAGG TGGGTTAGCT CACCCACCAC TTCCCGAGGC CAAGGGTTAC 
CCGAGCCCAG AGGTGATTCG GGAGTCCAGC AGCACCACGG GCATGGTCGT AGGCATCGTT 
GCAGCCGCTG CACTCTGCAT CCTAATCCTG CTCTACGCCA TGTACAAATA CAGAAACCGC 
GACGAAGGGT CTTACCACGT GGACGAGAGT CGTAACTACA TTAGTAATTC TGCCACGCAG 
CCCAATGGAG CTGCCGTCAA AGAAAAGCCC ATCGGCGTGC CCAAAAATAA AAAAGATAAA 
AAGAACAAGG ACAAGGAGTA CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333852.1
CDS	1001..2350
Translation	MLRLWPGGAPGGLASILLRISLRLALWLPPLTLGSALAEGPGELYVPQHMPHHLVPAARSHAPLRAGHAGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	NM_001346923.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346923.1

ORF Insert Sequence:

ATGCTGCGTC TGTGGCCCGG CGGGGCTCCT GGTGGTCTTG CTTCCATCCT GCTCCGCATC 
AGCCTGCGCC TAGCTCTCTG GCTGCCACCG CTGACTTTGG GGTCAGCTCT GGCAGAGGGG 
CCTGGGGAGC TGTACGTCCC TCAACATATG CCTCATCACC TCGTGCCTGC CGCCAGATCC 
CACGCCCCCC TGCGGGCAGG ACATGCGGGA ACCACATACA TATTCGGACG TGATGGAGGG 
CTTATTGTGT ACACATGGCC ACCCAACGAC AGACCGAGCA CACGAGCTGA CCGACTGGCT 
GTCGGTTTTA GTACCCAGCA GAAGGATGCA GTGCTGGTTA GGGTGGACAG CTCTTCTGGC 
CTGGGAGACT ACCTTCAGCT TCAGATTGAA AGAGGCAATA TCAAAGTGGT GTTCAATGTG 
GGCACCGATG ACATAAACAT TGAAGAAACT TCAAAGTTTG TAAATGATGG AAAGTACCAC 
ATAGTTCGCT TTACGAGAAG TGGTGGTAAC GCCACCTTGC AGGTGGATGA TCTGCCAGTC 
ATTGAGCGCT ACCCATCAGG CAACATTGAT AACGAACGCC TGGCGATTGC TAGACAGCGA 
ATCCCATATC GGCTTGGTCG AGTAGTTGAC GATTGGCTAC TCGACAAAGG TCGGCAGCTC 
ACCATCTTCA ACAGTCAGAC CACCATAAAG ATTGGAGGCT GGGAGAAGGG CAGCCGGCCC 
TTCCAGGGTC AGCTCTCAGG GCTTTACTAC AATGGGCTAA AAGTACTCAA CATGGCCGCT 
GAGGGTGACC CTAATGTGCG TGTGGAGGGT AGTGCCCGGC TGGTAGGAGA CATGCCCTCC 
TCTTCTATCA CGCCACAGTC CAGCGTCTCT GCTGCGGGAA ATCGTTCAGA AACGTCACCC 
TCCATCACTG ACATCACCAC TACAACGGCG TCCAACCGCC AGGGCAAGCA GACCACAACT 
CCACAGGACG ATCTTCTCGT AGCGTCTGCA GAATGCCCCA GCGATGATGA GGACATTGAC 
CCTTGCGACC CAAGTTCAGG TGGGTTAGCT CACCCACCAC TTCCCGAGGC CAAGGGTTAC 
CCGAGCCCAG AGGTGATTCG GGAGTCCAGC AGCACCACGG GCATGGTCGT AGGCATCGTT 
GCAGCCGCTG CACTCTGCAT CCTAATCCTG CTCTACGCCA TGTACAAATA CAGAAACCGC 
GACGAAGGGT CTTACCACGT GGACGAGAGT CGTAACTACA TTAGTAATTC TGCCACGCAG 
CCCAATGGAG CTGCCGTCAA AGAAAAGCCC ATCGGCGTGC CCAAAAATAA AAAAGATAAA 
AAGAACAAGG ACAAGGAGTA CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( NM_001346924.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001333853.1 Danio rerio neurexin 1a (nrxn1a), transcript variant 3, mRNA.

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CloneID	ODa98475
Clone ID Related Accession (Same CDS sequence)	NM_001346924.1
Accession Version	NM_001346924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-22
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX255907.8 and CR933760.7. On Nov 9, 2016 this sequence version replaced XM_017358470.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB214180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCGTC TGTGGCCCGG CGGGGCTCCT GGTGGTCTTG CTTCCATCCT GCTCCGCATC 
AGCCTGCGCC TAGCTCTCTG GCTGCCACCG CTGACTTTGG GGTCAGCTCT GGCAGAGGGG 
CCTGGGGAGC TGTACGTCCC TCAACATATG CCTCATCACC TCGTGCCTGC CGCCAGATCC 
CACGCCCCCC TGCGGGCAGG ACATGCGGGA ACCACATACA TATTCGGACG TGATGGAGGG 
CTTATTGTGT ACACATGGCC ACCCAACGAC AGACCGAGCA CACGAGCTGA CCGACTGGCT 
GTCGGTTTTA GTACCCAGCA GAAGGATGCA GTGCTGGTTA GGGTGGACAG CTCTTCTGGC 
CTGGGAGACT ACCTTCAGCT TCAGATTGAA AGAGGCAATA TCAAAGTGGT GTTCAATGTG 
GGCACCGATG ACATAAACAT TGAAGAAACT TCAAAGTTTG TAAATGATGG AAAGTACCAC 
ATAGTTCGCT TTACGAGAAG TGGTGGTAAC GCCACCTTGC AGGTGGATGA TCTGCCAGTC 
ATTGAGCGCT ACCCATCAGG TCGGCAGCTC ACCATCTTCA ACAGTCAGAC CACCATAAAG 
ATTGGAGGCT GGGAGAAGGG CAGCCGGCCC TTCCAGGGTC AGCTCTCAGG GCTTTACTAC 
AATGGGCTAA AAGTACTCAA CATGGCCGCT GAGGGTGACC CTAATGTGCG TGTGGAGGGT 
AGTGCCCGGC TGGTAGGAGA CATGCCCTCC TCTTCTATCA CGCCACAGTC CAGCGTCTCT 
GCTGCGGGAA ATCGTTCAGA AACGTCACCC TCCATCACTG ACATCACCAC TACAACGGCG 
TCCAACCGCC AGGGCAAGCA GACCACAACT CCACAGGACG ATCTTCTCGT AGCGTCTGCA 
GAATGCCCCA GCGATGATGA GGACATTGAC CCTTGCGACC CAAGTTCAGG TGGGTTAGCT 
CACCCACCAC TTCCCGAGGC CAAGGGTTAC CCGAGCCCAG AGGTGATTCG GGAGTCCAGC 
AGCACCACGG GCATGGTCGT AGGCATCGTT GCAGCCGCTG CACTCTGCAT CCTAATCCTG 
CTCTACGCCA TGTACAAATA CAGAAACCGC GACGAAGGGT CTTACCACGT GGACGAGAGT 
CGTAACTACA TTAGTAATTC TGCCACGCAG CCCAATGGAG CTGCCGTCAA AGAAAAGCCC 
ATCGGCGTGC CCAAAAATAA AAAAGATAAA AAGAACAAGG ACAAGGAGTA CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333853.1
CDS	1001..2260
Translation	MLRLWPGGAPGGLASILLRISLRLALWLPPLTLGSALAEGPGELYVPQHMPHHLVPAARSHAPLRAGHAGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	NM_001346924.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346924.1

ORF Insert Sequence:

ATGCTGCGTC TGTGGCCCGG CGGGGCTCCT GGTGGTCTTG CTTCCATCCT GCTCCGCATC 
AGCCTGCGCC TAGCTCTCTG GCTGCCACCG CTGACTTTGG GGTCAGCTCT GGCAGAGGGG 
CCTGGGGAGC TGTACGTCCC TCAACATATG CCTCATCACC TCGTGCCTGC CGCCAGATCC 
CACGCCCCCC TGCGGGCAGG ACATGCGGGA ACCACATACA TATTCGGACG TGATGGAGGG 
CTTATTGTGT ACACATGGCC ACCCAACGAC AGACCGAGCA CACGAGCTGA CCGACTGGCT 
GTCGGTTTTA GTACCCAGCA GAAGGATGCA GTGCTGGTTA GGGTGGACAG CTCTTCTGGC 
CTGGGAGACT ACCTTCAGCT TCAGATTGAA AGAGGCAATA TCAAAGTGGT GTTCAATGTG 
GGCACCGATG ACATAAACAT TGAAGAAACT TCAAAGTTTG TAAATGATGG AAAGTACCAC 
ATAGTTCGCT TTACGAGAAG TGGTGGTAAC GCCACCTTGC AGGTGGATGA TCTGCCAGTC 
ATTGAGCGCT ACCCATCAGG TCGGCAGCTC ACCATCTTCA ACAGTCAGAC CACCATAAAG 
ATTGGAGGCT GGGAGAAGGG CAGCCGGCCC TTCCAGGGTC AGCTCTCAGG GCTTTACTAC 
AATGGGCTAA AAGTACTCAA CATGGCCGCT GAGGGTGACC CTAATGTGCG TGTGGAGGGT 
AGTGCCCGGC TGGTAGGAGA CATGCCCTCC TCTTCTATCA CGCCACAGTC CAGCGTCTCT 
GCTGCGGGAA ATCGTTCAGA AACGTCACCC TCCATCACTG ACATCACCAC TACAACGGCG 
TCCAACCGCC AGGGCAAGCA GACCACAACT CCACAGGACG ATCTTCTCGT AGCGTCTGCA 
GAATGCCCCA GCGATGATGA GGACATTGAC CCTTGCGACC CAAGTTCAGG TGGGTTAGCT 
CACCCACCAC TTCCCGAGGC CAAGGGTTAC CCGAGCCCAG AGGTGATTCG GGAGTCCAGC 
AGCACCACGG GCATGGTCGT AGGCATCGTT GCAGCCGCTG CACTCTGCAT CCTAATCCTG 
CTCTACGCCA TGTACAAATA CAGAAACCGC GACGAAGGGT CTTACCACGT GGACGAGAGT 
CGTAACTACA TTAGTAATTC TGCCACGCAG CCCAATGGAG CTGCCGTCAA AGAAAAGCCC 
ATCGGCGTGC CCAAAAATAA AAAAGATAAA AAGAACAAGG ACAAGGAGTA CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480136.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335811.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X11, mRNA.

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CloneID	ODa148209
Clone ID Related Accession (Same CDS sequence)	XM_021480136.1
Accession Version	XM_021480136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGGTCTTGCG 
CACCTGATGA TGGGCGACCA AGAGAGAGAA GAATATATGG CCACATTCAA GGGTTCGGAG 
TATTTCTGTT ACGATCTGTC CCCGAACCCC ATCCAGAGCA GTAGCGATGA GATTACGCTC 
TCCTTCAAGA CCCTGCAGCG CAATGGACTG ATGCTTCACA CCGGAAAGTC TGCAGACTAC 
GTCAACCTGG CGCTGAAGAA CGGAGCCGTC TCGCTGGTCA TCAACCTGGG CTCTGGGGCC 
TTCGAGGCCC TGGTGGAGCC CGTCAATGGG AAATTTAATG ACAATGCCTG GCATGATGTG 
AAGGTTACCA GGAACCTTCG TCAGCACTCA GGCATTGGAC ACGCTATGGT TACAATATCC 
GTGGATGGGA TTCTGACCAC CACTGGATAT ACGCAAGAGG ACTACACCAT GCTAGGCTCG 
GATGACTTTT TCTATGTCGG CGGTAGTCCC AGCACTGCCG ATCTTCCTGG ATCACCTGTC 
AGCAACAACT TTATGGGCTG CTTGAAAGAG GTTGTTTACA AAAACAACGA TGTAAGACTT 
GAGCTGTCTC GACTGGCCAA GCTGGGCGAC CCAAAGATGA AGGTGAGCGG TGTGGTGGCC 
TTCAAATGTG AGAACGTGGC TACTCTCGAC CCTGTGACTT TTGAGACGCC TGAGTCATTC 
ATTACCCTTG ACAAGTGGTC CGCCAAAAAA GCCGGCTCCA TCTCTTTCGA CTTCCGCACC 
ACTGAACCCA ACGGCCTGCT CCTCTTCAGC CATGGAAAAC CCAAACCCCA GCAACAGAAA 
GACCCTAAGA GCCCTAAAAC ATTGAAGGTG GATTTCTTTG CCATTGAGAT GCTGGACGGC 
CACCTCTACC TTCTTCTCGA CATGGGCTCA GGCACCACCA AAACTAGAGC TGTCAACAAG 
AAAGTCAATG ACGGAGAATG GTACCACGTG GACTTCCAGA GAGATGGAAG ATCAGGCACC 
ATTTCCGTCA ACTCGATTCG CACTCCCTAC AACGCTCCTG GTGAGAGTGA GATACTGGAC 
CTGGACGATA AACTGTACCT GGGCGGCCTG CCAGAGGACC GGGCCGGTCT GATCTTCCCC 
ACGGAGGTGT GGACCGCTCT CCTAAATTAC GGCTATGTGG GCTGTGTGCG GGATCTGTTC 
ATGGATGGCC AGAGCAAGGA CATTCGGCGG ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG 
AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA CAGTGTCTGA GCAACCCCTG TCTGAACAGC 
GGCACCTGCA GAGAGGGCTG GAACCGCTAT GTGTGCGACT GCTCCGGGAC GGGATATCTG 
GGACGCTCCT GTGAGAGAGA TGCCACGATC CTCAGTTACG ATGGTAGTAA GTTCATGAAG 
ATCCAGCTGC CGGTGGTGAT GCACACAGAA GCGGAGGACG TGTCCCTGCG CTTTCGTTCT 
CAACGGGCCT ACGGTGTTCT AATGGCGACC ACATCGCAAA ACTCTGCTGA TACTCTGAGG 
CTGGAGCTGG ACGGAGGGCG GGTCCGACTC ACTGTCAATC TAGACTGTAT CAGGATAAAC 
TGTACTACCA GTAAAGGTCC AGAGACCATA TTTTCGGGCC AGAACTTGAA TGACAACGAA 
TGGCACACAG TTCGTGTCGT CAGAAGAGGC AAAAGTCTAA AGCTGATGGT GGACGACTTG 
CAGCCCTCTG AAGGTCAAAT CACTGGGGAC CACACGCAGC TGGAGTTCCA CAATGTGGAA 
ACGGGCATCG TTACCGAGAA GCGCTACATG CCCGCTGTGC CCTCCAACTT CATCGGACAC 
CTGCAGGGTT TGTCCTTCAA TGGCATGTCC TACATTGACT TGTGCAAGAA CGGCGATATC 
GACTACTGCG AACTGAACGC CATGATCGGC TACAGGAGCA TCGTGGCAGA CCCAGTGACC 
TTCAAGTCTC GCTCCAGCTA TGTGACTTTG CCCACCCTGC AGGCCTACTA TTCGATGCAC 
CTCTTCTTCC AGTTTAAGAC TACTTCTCCA GATGGGCTCA TCCTGTACAA CCGAGGAGAT 
GGCAATGACT TTATAGTGGT AGAGCTGGTT AAAGGATATC TCCATTACGT GTCAGATCTG 
GGAAATGGAG CTCATCTCAT CAAAGGCAAT TCCAACACAC CGCTAAATGA CAATCACTGG 
CACAACGTGA TGATCTCGCG AGATACAAAC AACCTCCATA CTGTCAAAAT AGACACCAAA 
ATCACCACAC AGACTACCAT GGGAGCCAAG AACCTGGATC TGAAAGGTGA TCTGTATGTT 
GGAGGTGTGG CTAAAGATAT GTATAAGGAC TTGCCAAAGC TGGTGCATTC AAAAGAGGGC 
TTCCAGGGCT GCCTGGCATC TGTGGACCTG AACGGCCGCT TGCCAGACCT GCAGTCAGAC 
GCACTGTCCA CCGCCGGCCA GGTGGAGAGA GGCTGCGAAG GTGAGGAAAA CGTTGCATTG 
CTGAAAGCTG ACTTGCAAGG CCCCAGCACT ACCTGTCAGG AGGACTCCTG CTCCAATCAG 
GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT AGTTGCGACT GCAGCATGAC ATCATATGGA 
GGGCCACTCT GCAATGACCC GGGAACCACA TACATATTCG GACGTGATGG AGGGCTTATT 
GTGTACACAT GGCCACCCAA CGACAGACCG AGCACACGAG CTGACCGACT GGCTGTCGGT 
TTTAGTACCC AGCAGAAGGA TGCAGTGCTG GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA 
GACTACCTTC AGCTTCAGAT TGAAAGAGGC AATATCAAAG TGGTGTTCAA TGTGGGCACC 
GATGACATAA ACATTGAAGA AACTTCAAAG TTTGTAAATG ATGGAAAGTA CCACATAGTT 
CGCTTTACGA GAAGTGGTGG TAACGCCACC TTGCAGGTGG ATGATCTGCC AGTCATTGAG 
CGCTACCCAT CAGGCAACAT TGATAACGAA CGCCTGGCGA TTGCTAGACA GCGAATCCCA 
TATCGGCTTG GTCGAGTAGT TGACGATTGG CTACTCGACA AAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGGTGGGTT AGCTCACCCA CCACTTCCCG AGGCCAAGGG TTACCCGAGC 
CCAGAGGTGA TTCGGGAGTC CAGCAGCACC ACGGGCATGG TCGTAGGCAT CGTTGCAGCC 
GCTGCACTCT GCATCCTAAT CCTGCTCTAC GCCATGTACA AATACAGAAA CCGCGACGAA 
GGGTCTTACC ACGTGGACGA GAGTCGTAAC TACATTAGTA ATTCTGCCAC GCAGCCCAAT 
GGAGCTGCCG TCAAAGAAAA GCCCATCGGC GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC 
AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335811.1
CDS	986..5449
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEGLAHLMMGDQEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480136.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480136.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGGTCTTGCG 
CACCTGATGA TGGGCGACCA AGAGAGAGAA GAATATATGG CCACATTCAA GGGTTCGGAG 
TATTTCTGTT ACGATCTGTC CCCGAACCCC ATCCAGAGCA GTAGCGATGA GATTACGCTC 
TCCTTCAAGA CCCTGCAGCG CAATGGACTG ATGCTTCACA CCGGAAAGTC TGCAGACTAC 
GTCAACCTGG CGCTGAAGAA CGGAGCCGTC TCGCTGGTCA TCAACCTGGG CTCTGGGGCC 
TTCGAGGCCC TGGTGGAGCC CGTCAATGGG AAATTTAATG ACAATGCCTG GCATGATGTG 
AAGGTTACCA GGAACCTTCG TCAGCACTCA GGCATTGGAC ACGCTATGGT TACAATATCC 
GTGGATGGGA TTCTGACCAC CACTGGATAT ACGCAAGAGG ACTACACCAT GCTAGGCTCG 
GATGACTTTT TCTATGTCGG CGGTAGTCCC AGCACTGCCG ATCTTCCTGG ATCACCTGTC 
AGCAACAACT TTATGGGCTG CTTGAAAGAG GTTGTTTACA AAAACAACGA TGTAAGACTT 
GAGCTGTCTC GACTGGCCAA GCTGGGCGAC CCAAAGATGA AGGTGAGCGG TGTGGTGGCC 
TTCAAATGTG AGAACGTGGC TACTCTCGAC CCTGTGACTT TTGAGACGCC TGAGTCATTC 
ATTACCCTTG ACAAGTGGTC CGCCAAAAAA GCCGGCTCCA TCTCTTTCGA CTTCCGCACC 
ACTGAACCCA ACGGCCTGCT CCTCTTCAGC CATGGAAAAC CCAAACCCCA GCAACAGAAA 
GACCCTAAGA GCCCTAAAAC ATTGAAGGTG GATTTCTTTG CCATTGAGAT GCTGGACGGC 
CACCTCTACC TTCTTCTCGA CATGGGCTCA GGCACCACCA AAACTAGAGC TGTCAACAAG 
AAAGTCAATG ACGGAGAATG GTACCACGTG GACTTCCAGA GAGATGGAAG ATCAGGCACC 
ATTTCCGTCA ACTCGATTCG CACTCCCTAC AACGCTCCTG GTGAGAGTGA GATACTGGAC 
CTGGACGATA AACTGTACCT GGGCGGCCTG CCAGAGGACC GGGCCGGTCT GATCTTCCCC 
ACGGAGGTGT GGACCGCTCT CCTAAATTAC GGCTATGTGG GCTGTGTGCG GGATCTGTTC 
ATGGATGGCC AGAGCAAGGA CATTCGGCGG ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG 
AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA CAGTGTCTGA GCAACCCCTG TCTGAACAGC 
GGCACCTGCA GAGAGGGCTG GAACCGCTAT GTGTGCGACT GCTCCGGGAC GGGATATCTG 
GGACGCTCCT GTGAGAGAGA TGCCACGATC CTCAGTTACG ATGGTAGTAA GTTCATGAAG 
ATCCAGCTGC CGGTGGTGAT GCACACAGAA GCGGAGGACG TGTCCCTGCG CTTTCGTTCT 
CAACGGGCCT ACGGTGTTCT AATGGCGACC ACATCGCAAA ACTCTGCTGA TACTCTGAGG 
CTGGAGCTGG ACGGAGGGCG GGTCCGACTC ACTGTCAATC TAGACTGTAT CAGGATAAAC 
TGTACTACCA GTAAAGGTCC AGAGACCATA TTTTCGGGCC AGAACTTGAA TGACAACGAA 
TGGCACACAG TTCGTGTCGT CAGAAGAGGC AAAAGTCTAA AGCTGATGGT GGACGACTTG 
CAGCCCTCTG AAGGTCAAAT CACTGGGGAC CACACGCAGC TGGAGTTCCA CAATGTGGAA 
ACGGGCATCG TTACCGAGAA GCGCTACATG CCCGCTGTGC CCTCCAACTT CATCGGACAC 
CTGCAGGGTT TGTCCTTCAA TGGCATGTCC TACATTGACT TGTGCAAGAA CGGCGATATC 
GACTACTGCG AACTGAACGC CATGATCGGC TACAGGAGCA TCGTGGCAGA CCCAGTGACC 
TTCAAGTCTC GCTCCAGCTA TGTGACTTTG CCCACCCTGC AGGCCTACTA TTCGATGCAC 
CTCTTCTTCC AGTTTAAGAC TACTTCTCCA GATGGGCTCA TCCTGTACAA CCGAGGAGAT 
GGCAATGACT TTATAGTGGT AGAGCTGGTT AAAGGATATC TCCATTACGT GTCAGATCTG 
GGAAATGGAG CTCATCTCAT CAAAGGCAAT TCCAACACAC CGCTAAATGA CAATCACTGG 
CACAACGTGA TGATCTCGCG AGATACAAAC AACCTCCATA CTGTCAAAAT AGACACCAAA 
ATCACCACAC AGACTACCAT GGGAGCCAAG AACCTGGATC TGAAAGGTGA TCTGTATGTT 
GGAGGTGTGG CTAAAGATAT GTATAAGGAC TTGCCAAAGC TGGTGCATTC AAAAGAGGGC 
TTCCAGGGCT GCCTGGCATC TGTGGACCTG AACGGCCGCT TGCCAGACCT GCAGTCAGAC 
GCACTGTCCA CCGCCGGCCA GGTGGAGAGA GGCTGCGAAG GTGAGGAAAA CGTTGCATTG 
CTGAAAGCTG ACTTGCAAGG CCCCAGCACT ACCTGTCAGG AGGACTCCTG CTCCAATCAG 
GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT AGTTGCGACT GCAGCATGAC ATCATATGGA 
GGGCCACTCT GCAATGACCC GGGAACCACA TACATATTCG GACGTGATGG AGGGCTTATT 
GTGTACACAT GGCCACCCAA CGACAGACCG AGCACACGAG CTGACCGACT GGCTGTCGGT 
TTTAGTACCC AGCAGAAGGA TGCAGTGCTG GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA 
GACTACCTTC AGCTTCAGAT TGAAAGAGGC AATATCAAAG TGGTGTTCAA TGTGGGCACC 
GATGACATAA ACATTGAAGA AACTTCAAAG TTTGTAAATG ATGGAAAGTA CCACATAGTT 
CGCTTTACGA GAAGTGGTGG TAACGCCACC TTGCAGGTGG ATGATCTGCC AGTCATTGAG 
CGCTACCCAT CAGGCAACAT TGATAACGAA CGCCTGGCGA TTGCTAGACA GCGAATCCCA 
TATCGGCTTG GTCGAGTAGT TGACGATTGG CTACTCGACA AAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGGTGGGTT AGCTCACCCA CCACTTCCCG AGGCCAAGGG TTACCCGAGC 
CCAGAGGTGA TTCGGGAGTC CAGCAGCACC ACGGGCATGG TCGTAGGCAT CGTTGCAGCC 
GCTGCACTCT GCATCCTAAT CCTGCTCTAC GCCATGTACA AATACAGAAA CCGCGACGAA 
GGGTCTTACC ACGTGGACGA GAGTCGTAAC TACATTAGTA ATTCTGCCAC GCAGCCCAAT 
GGAGCTGCCG TCAAAGAAAA GCCCATCGGC GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC 
AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480137.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335812.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa148210
Clone ID Related Accession (Same CDS sequence)	XM_021480137.1
Accession Version	XM_021480137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGCAACA TTGATAACGA ACGCCTGGCG ATTGCTAGAC AGCGAATCCC ATATCGGCTT 
GGTCGAGTAG TTGACGATTG GCTACTCGAC AAAGGTCGGC AGCTCACCAT CTTCAACAGT 
CAGACCACCA TAAAGATTGG AGGCTGGGAG AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC 
TCAGGGCTTT ACTACAATGG GCTAAAAGTA CTCAACATGG CCGCTGAGGG TGACCCTAAT 
GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA GGAGACATGC CCTCCTCTTC TATCACGCCA 
CAGTCCAGCG TCTCTGCTGC GGGAAATCGT TCAGAAACGT CACCCTCCAT CACTGACATC 
ACCACTACAA CGGCGTCCAA CCGCCAGGGC AAGCAGACCA CAACTCCACA GGACGATCTT 
CTCGTAGCGT CTGCAGAATG CCCCAGCGAT GATGAGGACA TTGACCCTTG CGACCCAAGT 
TCAGGTGGGT TAGCTCACCC ACCACTTCCC GAGGCCAAGG GTTACCCGAG CCCAGAGGTG 
ATTCGGGAGT CCAGCAGCAC CACGGGCATG GTCGTAGGCA TCGTTGCAGC CGCTGCACTC 
TGCATCCTAA TCCTGCTCTA CGCCATGTAC AAATACAGAA ACCGCGACGA AGGGTCTTAC 
CACGTGGACG AGAGTCGTAA CTACATTAGT AATTCTGCCA CGCAGCCCAA TGGAGCTGCC 
GTCAAAGAAA AGCCCATCGG CGTGCCCAAA AATAAAAAAG ATAAAAAGAA CAAGGACAAG 
GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335812.1
CDS	985..5439
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480137.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480137.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGCAACA TTGATAACGA ACGCCTGGCG ATTGCTAGAC AGCGAATCCC ATATCGGCTT 
GGTCGAGTAG TTGACGATTG GCTACTCGAC AAAGGTCGGC AGCTCACCAT CTTCAACAGT 
CAGACCACCA TAAAGATTGG AGGCTGGGAG AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC 
TCAGGGCTTT ACTACAATGG GCTAAAAGTA CTCAACATGG CCGCTGAGGG TGACCCTAAT 
GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA GGAGACATGC CCTCCTCTTC TATCACGCCA 
CAGTCCAGCG TCTCTGCTGC GGGAAATCGT TCAGAAACGT CACCCTCCAT CACTGACATC 
ACCACTACAA CGGCGTCCAA CCGCCAGGGC AAGCAGACCA CAACTCCACA GGACGATCTT 
CTCGTAGCGT CTGCAGAATG CCCCAGCGAT GATGAGGACA TTGACCCTTG CGACCCAAGT 
TCAGGTGGGT TAGCTCACCC ACCACTTCCC GAGGCCAAGG GTTACCCGAG CCCAGAGGTG 
ATTCGGGAGT CCAGCAGCAC CACGGGCATG GTCGTAGGCA TCGTTGCAGC CGCTGCACTC 
TGCATCCTAA TCCTGCTCTA CGCCATGTAC AAATACAGAA ACCGCGACGA AGGGTCTTAC 
CACGTGGACG AGAGTCGTAA CTACATTAGT AATTCTGCCA CGCAGCCCAA TGGAGCTGCC 
GTCAAAGAAA AGCCCATCGG CGTGCCCAAA AATAAAAAAG ATAAAAAGAA CAAGGACAAG 
GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480138.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335813.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa148211
Clone ID Related Accession (Same CDS sequence)	XM_021480138.1
Accession Version	XM_021480138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGGTGGGTT AGCTCACCCA 
CCACTTCCCG AGGCCAAGGG TTACCCGAGC CCAGAGGTGA TTCGGGAGTC CAGCAGCACC 
ACGGGCATGG TCGTAGGCAT CGTTGCAGCC GCTGCACTCT GCATCCTAAT CCTGCTCTAC 
GCCATGTACA AATACAGAAA CCGCGACGAA GGGTCTTACC ACGTGGACGA GAGTCGTAAC 
TACATTAGTA ATTCTGCCAC GCAGCCCAAT GGAGCTGCCG TCAAAGAAAA GCCCATCGGC 
GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335813.1
CDS	986..5419
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480138.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480138.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGGTGGGTT AGCTCACCCA 
CCACTTCCCG AGGCCAAGGG TTACCCGAGC CCAGAGGTGA TTCGGGAGTC CAGCAGCACC 
ACGGGCATGG TCGTAGGCAT CGTTGCAGCC GCTGCACTCT GCATCCTAAT CCTGCTCTAC 
GCCATGTACA AATACAGAAA CCGCGACGAA GGGTCTTACC ACGTGGACGA GAGTCGTAAC 
TACATTAGTA ATTCTGCCAC GCAGCCCAAT GGAGCTGCCG TCAAAGAAAA GCCCATCGGC 
GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480139.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335814.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X15, mRNA.

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CloneID	ODa148212
Clone ID Related Accession (Same CDS sequence)	XM_021480139.1
Accession Version	XM_021480139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGCCCCAG CACTACCTGT 
CAGGAGGACT CCTGCTCCAA TCAGGGCGTG TGTCTGCAGC AGTGGGAGGG GTTTAGTTGC 
GACTGCAGCA TGACATCATA TGGAGGGCCA CTCTGCAATG ACCCGGGAAC CACATACATA 
TTCGGACGTG ATGGAGGGCT TATTGTGTAC ACATGGCCAC CCAACGACAG ACCGAGCACA 
CGAGCTGACC GACTGGCTGT CGGTTTTAGT ACCCAGCAGA AGGATGCAGT GCTGGTTAGG 
GTGGACAGCT CTTCTGGCCT GGGAGACTAC CTTCAGCTTC AGATTGAAAG AGGCAATATC 
AAAGTGGTGT TCAATGTGGG CACCGATGAC ATAAACATTG AAGAAACTTC AAAGTTTGTA 
AATGATGGAA AGTACCACAT AGTTCGCTTT ACGAGAAGTG GTGGTAACGC CACCTTGCAG 
GTGGATGATC TGCCAGTCAT TGAGCGCTAC CCATCAGGCA ACATTGATAA CGAACGCCTG 
GCGATTGCTA GACAGCGAAT CCCATATCGG CTTGGTCGAG TAGTTGACGA TTGGCTACTC 
GACAAAGGTC GGCAGCTCAC CATCTTCAAC AGTCAGACCA CCATAAAGAT TGGAGGCTGG 
GAGAAGGGCA GCCGGCCCTT CCAGGGTCAG CTCTCAGGGC TTTACTACAA TGGGCTAAAA 
GTACTCAACA TGGCCGCTGA GGGTGACCCT AATGTGCGTG TGGAGGGTAG TGCCCGGCTG 
GTAGGAGACA TGCCCTCCTC TTCTATCACG CCACAGTCCA GCGTCTCTGC TGCGGGAAAT 
CGTTCAGAAA CGTCACCCTC CATCACTGAC ATCACCACTA CAACGGCGTC CAACCGCCAG 
GGCAAGCAGA CCACAACTCC ACAGGACGAT CTTCTCGTAG CGTCTGCAGA ATGCCCCAGC 
GATGATGAGG ACATTGACCC TTGCGACCCA AGTTCAGGTG GGTTAGCTCA CCCACCACTT 
CCCGAGGCCA AGGGTTACCC GAGCCCAGAG GTGATTCGGG AGTCCAGCAG CACCACGGGC 
ATGGTCGTAG GCATCGTTGC AGCCGCTGCA CTCTGCATCC TAATCCTGCT CTACGCCATG 
TACAAATACA GAAACCGCGA CGAAGGGTCT TACCACGTGG ACGAGAGTCG TAACTACATT 
AGTAATTCTG CCACGCAGCC CAATGGAGCT GCCGTCAAAG AAAAGCCCAT CGGCGTGCCC 
AAAAATAAAA AAGATAAAAA GAACAAGGAC AAGGAGTACT ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335814.1
CDS	986..5413
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLGKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480139.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480139.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGCCCCAG CACTACCTGT 
CAGGAGGACT CCTGCTCCAA TCAGGGCGTG TGTCTGCAGC AGTGGGAGGG GTTTAGTTGC 
GACTGCAGCA TGACATCATA TGGAGGGCCA CTCTGCAATG ACCCGGGAAC CACATACATA 
TTCGGACGTG ATGGAGGGCT TATTGTGTAC ACATGGCCAC CCAACGACAG ACCGAGCACA 
CGAGCTGACC GACTGGCTGT CGGTTTTAGT ACCCAGCAGA AGGATGCAGT GCTGGTTAGG 
GTGGACAGCT CTTCTGGCCT GGGAGACTAC CTTCAGCTTC AGATTGAAAG AGGCAATATC 
AAAGTGGTGT TCAATGTGGG CACCGATGAC ATAAACATTG AAGAAACTTC AAAGTTTGTA 
AATGATGGAA AGTACCACAT AGTTCGCTTT ACGAGAAGTG GTGGTAACGC CACCTTGCAG 
GTGGATGATC TGCCAGTCAT TGAGCGCTAC CCATCAGGCA ACATTGATAA CGAACGCCTG 
GCGATTGCTA GACAGCGAAT CCCATATCGG CTTGGTCGAG TAGTTGACGA TTGGCTACTC 
GACAAAGGTC GGCAGCTCAC CATCTTCAAC AGTCAGACCA CCATAAAGAT TGGAGGCTGG 
GAGAAGGGCA GCCGGCCCTT CCAGGGTCAG CTCTCAGGGC TTTACTACAA TGGGCTAAAA 
GTACTCAACA TGGCCGCTGA GGGTGACCCT AATGTGCGTG TGGAGGGTAG TGCCCGGCTG 
GTAGGAGACA TGCCCTCCTC TTCTATCACG CCACAGTCCA GCGTCTCTGC TGCGGGAAAT 
CGTTCAGAAA CGTCACCCTC CATCACTGAC ATCACCACTA CAACGGCGTC CAACCGCCAG 
GGCAAGCAGA CCACAACTCC ACAGGACGAT CTTCTCGTAG CGTCTGCAGA ATGCCCCAGC 
GATGATGAGG ACATTGACCC TTGCGACCCA AGTTCAGGTG GGTTAGCTCA CCCACCACTT 
CCCGAGGCCA AGGGTTACCC GAGCCCAGAG GTGATTCGGG AGTCCAGCAG CACCACGGGC 
ATGGTCGTAG GCATCGTTGC AGCCGCTGCA CTCTGCATCC TAATCCTGCT CTACGCCATG 
TACAAATACA GAAACCGCGA CGAAGGGTCT TACCACGTGG ACGAGAGTCG TAACTACATT 
AGTAATTCTG CCACGCAGCC CAATGGAGCT GCCGTCAAAG AAAAGCCCAT CGGCGTGCCC 
AAAAATAAAA AAGATAAAAA GAACAAGGAC AAGGAGTACT ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480140.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335815.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X16, mRNA.

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CloneID	ODa148213
Clone ID Related Accession (Same CDS sequence)	XM_021480140.1
Accession Version	XM_021480140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG AGAGAGAAGA ATATATGGCC 
ACATTCAAGG GTTCGGAGTA TTTCTGTTAC GATCTGTCCC CGAACCCCAT CCAGAGCAGT 
AGCGATGAGA TTACGCTCTC CTTCAAGACC CTGCAGCGCA ATGGACTGAT GCTTCACACC 
GGAAAGTCTG CAGACTACGT CAACCTGGCG CTGAAGAACG GAGCCGTCTC GCTGGTCATC 
AACCTGGGCT CTGGGGCCTT CGAGGCCCTG GTGGAGCCCG TCAATGGGAA ATTTAATGAC 
AATGCCTGGC ATGATGTGAA GGTTACCAGG AACCTTCGTC AGGTTACAAT ATCCGTGGAT 
GGGATTCTGA CCACCACTGG ATATACGCAA GAGGACTACA CCATGCTAGG CTCGGATGAC 
TTTTTCTATG TCGGCGGTAG TCCCAGCACT GCCGATCTTC CTGGATCACC TGTCAGCAAC 
AACTTTATGG GCTGCTTGAA AGAGGTTGTT TACAAAAACA ACGATGTAAG ACTTGAGCTG 
TCTCGACTGG CCAAGCTGGG CGACCCAAAG ATGAAGGTGA GCGGTGTGGT GGCCTTCAAA 
TGTGAGAACG TGGCTACTCT CGACCCTGTG ACTTTTGAGA CGCCTGAGTC ATTCATTACC 
CTTGACAAGT GGTCCGCCAA AAAAGCCGGC TCCATCTCTT TCGACTTCCG CACCACTGAA 
CCCAACGGCC TGCTCCTCTT CAGCCATGGA AAACCCAAAC CCCAGCAACA GAAAGACCCT 
AAGAGCCCTA AAACATTGAA GGTGGATTTC TTTGCCATTG AGATGCTGGA CGGCCACCTC 
TACCTTCTTC TCGACATGGG CTCAGGCACC ACCAAAACTA GAGCTGTCAA CAAGAAAGTC 
AATGACGGAG AATGGTACCA CGTGGACTTC CAGAGAGATG GAAGATCAGG CACCATTTCC 
GTCAACTCGA TTCGCACTCC CTACAACGCT CCTGGTGAGA GTGAGATACT GGACCTGGAC 
GATAAACTGT ACCTGGGCGG CCTGCCAGAG GACCGGGCCG GTCTGATCTT CCCCACGGAG 
GTGTGGACCG CTCTCCTAAA TTACGGCTAT GTGGGCTGTG TGCGGGATCT GTTCATGGAT 
GGCCAGAGCA AGGACATTCG GCGGATAGCA GAGGCTCAGA GAGCGGTCGG GGTGAAGCCG 
TCTTGCTCTA AAGAACCCCC CAAACAGTGT CTGAGCAACC CCTGTCTGAA CAGCGGCACC 
TGCAGAGAGG GCTGGAACCG CTATGTGTGC GACTGCTCCG GGACGGGATA TCTGGGACGC 
TCCTGTGAGA GAGATGCCAC GATCCTCAGT TACGATGGTA GTAAGTTCAT GAAGATCCAG 
CTGCCGGTGG TGATGCACAC AGAAGCGGAG GACGTGTCCC TGCGCTTTCG TTCTCAACGG 
GCCTACGGTG TTCTAATGGC GACCACATCG CAAAACTCTG CTGATACTCT GAGGCTGGAG 
CTGGACGGAG GGCGGGTCCG ACTCACTGTC AATCTAGACT GTATCAGGAT AAACTGTACT 
ACCAGTAAAG GTCCAGAGAC CATATTTTCG GGCCAGAACT TGAATGACAA CGAATGGCAC 
ACAGTTCGTG TCGTCAGAAG AGGCAAAAGT CTAAAGCTGA TGGTGGACGA CTTGCAGCCC 
TCTGAAGGTC AAATCACTGG GGACCACACG CAGCTGGAGT TCCACAATGT GGAAACGGGC 
ATCGTTACCG AGAAGCGCTA CATGCCCGCT GTGCCCTCCA ACTTCATCGG ACACCTGCAG 
GGTTTGTCCT TCAATGGCAT GTCCTACATT GACTTGTGCA AGAACGGCGA TATCGACTAC 
TGCGAACTGA ACGCCATGAT CGGCTACAGG AGCATCGTGG CAGACCCAGT GACCTTCAAG 
TCTCGCTCCA GCTATGTGAC TTTGCCCACC CTGCAGGCCT ACTATTCGAT GCACCTCTTC 
TTCCAGTTTA AGACTACTTC TCCAGATGGG CTCATCCTGT ACAACCGAGG AGATGGCAAT 
GACTTTATAG TGGTAGAGCT GGTTAAAGGA TATCTCCATT ACGTGTCAGA TCTGGGAAAT 
GGAGCTCATC TCATCAAAGG CAATTCCAAC ACACCGCTAA ATGACAATCA CTGGCACAAC 
GTGATGATCT CGCGAGATAC AAACAACCTC CATACTGTCA AAATAGACAC CAAAATCACC 
ACACAGACTA CCATGGGAGC CAAGAACCTG GATCTGAAAG GTGATCTGTA TGTTGGAGGT 
GTGGCTAAAG ATATGTATAA GGACTTGCCA AAGCTGGTGC ATTCAAAAGA GGGCTTCCAG 
GGCTGCCTGG CATCTGTGGA CCTGAACGGC CGCTTGCCAG ACCTGCAGTC AGACGCACTG 
TCCACCGCCG GCCAGGTGGA GAGAGGCTGC GAAGGCCCCA GCACTACCTG TCAGGAGGAC 
TCCTGCTCCA ATCAGGGCGT GTGTCTGCAG CAGTGGGAGG GGTTTAGTTG CGACTGCAGC 
ATGACATCAT ATGGAGGGCC ACTCTGCAAT GACCCGGGAA CCACATACAT ATTCGGACGT 
GATGGAGGGC TTATTGTGTA CACATGGCCA CCCAACGACA GACCGAGCAC ACGAGCTGAC 
CGACTGGCTG TCGGTTTTAG TACCCAGCAG AAGGATGCAG TGCTGGTTAG GGTGGACAGC 
TCTTCTGGCC TGGGAGACTA CCTTCAGCTT CAGATTGAAA GAGGCAATAT CAAAGTGGTG 
TTCAATGTGG GCACCGATGA CATAAACATT GAAGAAACTT CAAAGTTTGT AAATGATGGA 
AAGTACCACA TAGTTCGCTT TACGAGAAGT GGTGGTAACG CCACCTTGCA GGTGGATGAT 
CTGCCAGTCA TTGAGCGCTA CCCATCAGGC AACATTGATA ACGAACGCCT GGCGATTGCT 
AGACAGCGAA TCCCATATCG GCTTGGTCGA GTAGTTGACG ATTGGCTACT CGACAAAGGT 
CGGCAGCTCA CCATCTTCAA CAGTCAGACC ACCATAAAGA TTGGAGGCTG GGAGAAGGGC 
AGCCGGCCCT TCCAGGGTCA GCTCTCAGGG CTTTACTACA ATGGGCTAAA AGTACTCAAC 
ATGGCCGCTG AGGGTGACCC TAATGTGCGT GTGGAGGGTA GTGCCCGGCT GGTAGGAGAC 
ATGCCCTCCT CTTCTATCAC GCCACAGTCC AGCGTCTCTG CTGCGGGAAA TCGTTCAGAA 
ACGTCACCCT CCATCACTGA CATCACCACT ACAACGGCGT CCAACCGCCA GGGCAAGCAG 
ACCACAACTC CACAGGACGA TCTTCTCGTA GCGTCTGCAG AATGCCCCAG CGATGATGAG 
GACATTGACC CTTGCGACCC AAGTTCAGGT GGGTTAGCTC ACCCACCACT TCCCGAGGCC 
AAGGGTTACC CGAGCCCAGA GGTGATTCGG GAGTCCAGCA GCACCACGGG CATGGTCGTA 
GGCATCGTTG CAGCCGCTGC ACTCTGCATC CTAATCCTGC TCTACGCCAT GTACAAATAC 
AGAAACCGCG ACGAAGGGTC TTACCACGTG GACGAGAGTC GTAACTACAT TAGTAATTCT 
GCCACGCAGC CCAATGGAGC TGCCGTCAAA GAAAAGCCCA TCGGCGTGCC CAAAAATAAA 
AAAGATAAAA AGAACAAGGA CAAGGAGTAC TACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335815.1
CDS	985..5403
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480140.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480140.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG AGAGAGAAGA ATATATGGCC 
ACATTCAAGG GTTCGGAGTA TTTCTGTTAC GATCTGTCCC CGAACCCCAT CCAGAGCAGT 
AGCGATGAGA TTACGCTCTC CTTCAAGACC CTGCAGCGCA ATGGACTGAT GCTTCACACC 
GGAAAGTCTG CAGACTACGT CAACCTGGCG CTGAAGAACG GAGCCGTCTC GCTGGTCATC 
AACCTGGGCT CTGGGGCCTT CGAGGCCCTG GTGGAGCCCG TCAATGGGAA ATTTAATGAC 
AATGCCTGGC ATGATGTGAA GGTTACCAGG AACCTTCGTC AGGTTACAAT ATCCGTGGAT 
GGGATTCTGA CCACCACTGG ATATACGCAA GAGGACTACA CCATGCTAGG CTCGGATGAC 
TTTTTCTATG TCGGCGGTAG TCCCAGCACT GCCGATCTTC CTGGATCACC TGTCAGCAAC 
AACTTTATGG GCTGCTTGAA AGAGGTTGTT TACAAAAACA ACGATGTAAG ACTTGAGCTG 
TCTCGACTGG CCAAGCTGGG CGACCCAAAG ATGAAGGTGA GCGGTGTGGT GGCCTTCAAA 
TGTGAGAACG TGGCTACTCT CGACCCTGTG ACTTTTGAGA CGCCTGAGTC ATTCATTACC 
CTTGACAAGT GGTCCGCCAA AAAAGCCGGC TCCATCTCTT TCGACTTCCG CACCACTGAA 
CCCAACGGCC TGCTCCTCTT CAGCCATGGA AAACCCAAAC CCCAGCAACA GAAAGACCCT 
AAGAGCCCTA AAACATTGAA GGTGGATTTC TTTGCCATTG AGATGCTGGA CGGCCACCTC 
TACCTTCTTC TCGACATGGG CTCAGGCACC ACCAAAACTA GAGCTGTCAA CAAGAAAGTC 
AATGACGGAG AATGGTACCA CGTGGACTTC CAGAGAGATG GAAGATCAGG CACCATTTCC 
GTCAACTCGA TTCGCACTCC CTACAACGCT CCTGGTGAGA GTGAGATACT GGACCTGGAC 
GATAAACTGT ACCTGGGCGG CCTGCCAGAG GACCGGGCCG GTCTGATCTT CCCCACGGAG 
GTGTGGACCG CTCTCCTAAA TTACGGCTAT GTGGGCTGTG TGCGGGATCT GTTCATGGAT 
GGCCAGAGCA AGGACATTCG GCGGATAGCA GAGGCTCAGA GAGCGGTCGG GGTGAAGCCG 
TCTTGCTCTA AAGAACCCCC CAAACAGTGT CTGAGCAACC CCTGTCTGAA CAGCGGCACC 
TGCAGAGAGG GCTGGAACCG CTATGTGTGC GACTGCTCCG GGACGGGATA TCTGGGACGC 
TCCTGTGAGA GAGATGCCAC GATCCTCAGT TACGATGGTA GTAAGTTCAT GAAGATCCAG 
CTGCCGGTGG TGATGCACAC AGAAGCGGAG GACGTGTCCC TGCGCTTTCG TTCTCAACGG 
GCCTACGGTG TTCTAATGGC GACCACATCG CAAAACTCTG CTGATACTCT GAGGCTGGAG 
CTGGACGGAG GGCGGGTCCG ACTCACTGTC AATCTAGACT GTATCAGGAT AAACTGTACT 
ACCAGTAAAG GTCCAGAGAC CATATTTTCG GGCCAGAACT TGAATGACAA CGAATGGCAC 
ACAGTTCGTG TCGTCAGAAG AGGCAAAAGT CTAAAGCTGA TGGTGGACGA CTTGCAGCCC 
TCTGAAGGTC AAATCACTGG GGACCACACG CAGCTGGAGT TCCACAATGT GGAAACGGGC 
ATCGTTACCG AGAAGCGCTA CATGCCCGCT GTGCCCTCCA ACTTCATCGG ACACCTGCAG 
GGTTTGTCCT TCAATGGCAT GTCCTACATT GACTTGTGCA AGAACGGCGA TATCGACTAC 
TGCGAACTGA ACGCCATGAT CGGCTACAGG AGCATCGTGG CAGACCCAGT GACCTTCAAG 
TCTCGCTCCA GCTATGTGAC TTTGCCCACC CTGCAGGCCT ACTATTCGAT GCACCTCTTC 
TTCCAGTTTA AGACTACTTC TCCAGATGGG CTCATCCTGT ACAACCGAGG AGATGGCAAT 
GACTTTATAG TGGTAGAGCT GGTTAAAGGA TATCTCCATT ACGTGTCAGA TCTGGGAAAT 
GGAGCTCATC TCATCAAAGG CAATTCCAAC ACACCGCTAA ATGACAATCA CTGGCACAAC 
GTGATGATCT CGCGAGATAC AAACAACCTC CATACTGTCA AAATAGACAC CAAAATCACC 
ACACAGACTA CCATGGGAGC CAAGAACCTG GATCTGAAAG GTGATCTGTA TGTTGGAGGT 
GTGGCTAAAG ATATGTATAA GGACTTGCCA AAGCTGGTGC ATTCAAAAGA GGGCTTCCAG 
GGCTGCCTGG CATCTGTGGA CCTGAACGGC CGCTTGCCAG ACCTGCAGTC AGACGCACTG 
TCCACCGCCG GCCAGGTGGA GAGAGGCTGC GAAGGCCCCA GCACTACCTG TCAGGAGGAC 
TCCTGCTCCA ATCAGGGCGT GTGTCTGCAG CAGTGGGAGG GGTTTAGTTG CGACTGCAGC 
ATGACATCAT ATGGAGGGCC ACTCTGCAAT GACCCGGGAA CCACATACAT ATTCGGACGT 
GATGGAGGGC TTATTGTGTA CACATGGCCA CCCAACGACA GACCGAGCAC ACGAGCTGAC 
CGACTGGCTG TCGGTTTTAG TACCCAGCAG AAGGATGCAG TGCTGGTTAG GGTGGACAGC 
TCTTCTGGCC TGGGAGACTA CCTTCAGCTT CAGATTGAAA GAGGCAATAT CAAAGTGGTG 
TTCAATGTGG GCACCGATGA CATAAACATT GAAGAAACTT CAAAGTTTGT AAATGATGGA 
AAGTACCACA TAGTTCGCTT TACGAGAAGT GGTGGTAACG CCACCTTGCA GGTGGATGAT 
CTGCCAGTCA TTGAGCGCTA CCCATCAGGC AACATTGATA ACGAACGCCT GGCGATTGCT 
AGACAGCGAA TCCCATATCG GCTTGGTCGA GTAGTTGACG ATTGGCTACT CGACAAAGGT 
CGGCAGCTCA CCATCTTCAA CAGTCAGACC ACCATAAAGA TTGGAGGCTG GGAGAAGGGC 
AGCCGGCCCT TCCAGGGTCA GCTCTCAGGG CTTTACTACA ATGGGCTAAA AGTACTCAAC 
ATGGCCGCTG AGGGTGACCC TAATGTGCGT GTGGAGGGTA GTGCCCGGCT GGTAGGAGAC 
ATGCCCTCCT CTTCTATCAC GCCACAGTCC AGCGTCTCTG CTGCGGGAAA TCGTTCAGAA 
ACGTCACCCT CCATCACTGA CATCACCACT ACAACGGCGT CCAACCGCCA GGGCAAGCAG 
ACCACAACTC CACAGGACGA TCTTCTCGTA GCGTCTGCAG AATGCCCCAG CGATGATGAG 
GACATTGACC CTTGCGACCC AAGTTCAGGT GGGTTAGCTC ACCCACCACT TCCCGAGGCC 
AAGGGTTACC CGAGCCCAGA GGTGATTCGG GAGTCCAGCA GCACCACGGG CATGGTCGTA 
GGCATCGTTG CAGCCGCTGC ACTCTGCATC CTAATCCTGC TCTACGCCAT GTACAAATAC 
AGAAACCGCG ACGAAGGGTC TTACCACGTG GACGAGAGTC GTAACTACAT TAGTAATTCT 
GCCACGCAGC CCAATGGAGC TGCCGTCAAA GAAAAGCCCA TCGGCGTGCC CAAAAATAAA 
AAAGATAAAA AGAACAAGGA CAAGGAGTAC TACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480141.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335816.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X20, mRNA.

Price & Availability↑

CloneID	ODa148214
Clone ID Related Accession (Same CDS sequence)	XM_021480141.1
Accession Version	XM_021480141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGCCCCAG CACTACCTGT 
CAGGAGGACT CCTGCTCCAA TCAGGGCGTG TGTCTGCAGC AGTGGGAGGG GTTTAGTTGC 
GACTGCAGCA TGACATCATA TGGAGGGCCA CTCTGCAATG ACCCGGGAAC CACATACATA 
TTCGGACGTG ATGGAGGGCT TATTGTGTAC ACATGGCCAC CCAACGACAG ACCGAGCACA 
CGAGCTGACC GACTGGCTGT CGGTTTTAGT ACCCAGCAGA AGGATGCAGT GCTGGTTAGG 
GTGGACAGCT CTTCTGGCCT GGGAGACTAC CTTCAGCTTC AGATTGAAAG AGGCAATATC 
AAAGTGGTGT TCAATGTGGG CACCGATGAC ATAAACATTG AAGAAACTTC AAAGTTTGTA 
AATGATGGAA AGTACCACAT AGTTCGCTTT ACGAGAAGTG GTGGTAACGC CACCTTGCAG 
GTGGATGATC TGCCAGTCAT TGAGCGCTAC CCATCAGGTC GGCAGCTCAC CATCTTCAAC 
AGTCAGACCA CCATAAAGAT TGGAGGCTGG GAGAAGGGCA GCCGGCCCTT CCAGGGTCAG 
CTCTCAGGGC TTTACTACAA TGGGCTAAAA GTACTCAACA TGGCCGCTGA GGGTGACCCT 
AATGTGCGTG TGGAGGGTAG TGCCCGGCTG GTAGGAGACA TGCCCTCCTC TTCTATCACG 
CCACAGTCCA GCGTCTCTGC TGCGGGAAAT CGTTCAGAAA CGTCACCCTC CATCACTGAC 
ATCACCACTA CAACGGCGTC CAACCGCCAG GGCAAGCAGA CCACAACTCC ACAGGACGAT 
CTTCTCGTAG CGTCTGCAGA ATGCCCCAGC GATGATGAGG ACATTGACCC TTGCGACCCA 
AGTTCAGGTG GGTTAGCTCA CCCACCACTT CCCGAGGCCA AGGGTTACCC GAGCCCAGAG 
GTGATTCGGG AGTCCAGCAG CACCACGGGC ATGGTCGTAG GCATCGTTGC AGCCGCTGCA 
CTCTGCATCC TAATCCTGCT CTACGCCATG TACAAATACA GAAACCGCGA CGAAGGGTCT 
TACCACGTGG ACGAGAGTCG TAACTACATT AGTAATTCTG CCACGCAGCC CAATGGAGCT 
GCCGTCAAAG AAAAGCCCAT CGGCGTGCCC AAAAATAAAA AAGATAAAAA GAACAAGGAC 
AAGGAGTACT ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335816.1
CDS	986..5323
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLGKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480141.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480141.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGCCCCAG CACTACCTGT 
CAGGAGGACT CCTGCTCCAA TCAGGGCGTG TGTCTGCAGC AGTGGGAGGG GTTTAGTTGC 
GACTGCAGCA TGACATCATA TGGAGGGCCA CTCTGCAATG ACCCGGGAAC CACATACATA 
TTCGGACGTG ATGGAGGGCT TATTGTGTAC ACATGGCCAC CCAACGACAG ACCGAGCACA 
CGAGCTGACC GACTGGCTGT CGGTTTTAGT ACCCAGCAGA AGGATGCAGT GCTGGTTAGG 
GTGGACAGCT CTTCTGGCCT GGGAGACTAC CTTCAGCTTC AGATTGAAAG AGGCAATATC 
AAAGTGGTGT TCAATGTGGG CACCGATGAC ATAAACATTG AAGAAACTTC AAAGTTTGTA 
AATGATGGAA AGTACCACAT AGTTCGCTTT ACGAGAAGTG GTGGTAACGC CACCTTGCAG 
GTGGATGATC TGCCAGTCAT TGAGCGCTAC CCATCAGGTC GGCAGCTCAC CATCTTCAAC 
AGTCAGACCA CCATAAAGAT TGGAGGCTGG GAGAAGGGCA GCCGGCCCTT CCAGGGTCAG 
CTCTCAGGGC TTTACTACAA TGGGCTAAAA GTACTCAACA TGGCCGCTGA GGGTGACCCT 
AATGTGCGTG TGGAGGGTAG TGCCCGGCTG GTAGGAGACA TGCCCTCCTC TTCTATCACG 
CCACAGTCCA GCGTCTCTGC TGCGGGAAAT CGTTCAGAAA CGTCACCCTC CATCACTGAC 
ATCACCACTA CAACGGCGTC CAACCGCCAG GGCAAGCAGA CCACAACTCC ACAGGACGAT 
CTTCTCGTAG CGTCTGCAGA ATGCCCCAGC GATGATGAGG ACATTGACCC TTGCGACCCA 
AGTTCAGGTG GGTTAGCTCA CCCACCACTT CCCGAGGCCA AGGGTTACCC GAGCCCAGAG 
GTGATTCGGG AGTCCAGCAG CACCACGGGC ATGGTCGTAG GCATCGTTGC AGCCGCTGCA 
CTCTGCATCC TAATCCTGCT CTACGCCATG TACAAATACA GAAACCGCGA CGAAGGGTCT 
TACCACGTGG ACGAGAGTCG TAACTACATT AGTAATTCTG CCACGCAGCC CAATGGAGCT 
GCCGTCAAAG AAAAGCCCAT CGGCGTGCCC AAAAATAAAA AAGATAAAAA GAACAAGGAC 
AAGGAGTACT ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480142.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335817.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ODa148215
Clone ID Related Accession (Same CDS sequence)	XM_021480142.1
Accession Version	XM_021480142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGGTTACA 
ATATCCGTGG ATGGGATTCT GACCACCACT GGATATACGC AAGAGGACTA CACCATGCTA 
GGCTCGGATG ACTTTTTCTA TGTCGGCGGT AGTCCCAGCA CTGCCGATCT TCCTGGATCA 
CCTGTCAGCA ACAACTTTAT GGGCTGCTTG AAAGAGGTTG TTTACAAAAA CAACGATGTA 
AGACTTGAGC TGTCTCGACT GGCCAAGCTG GGCGACCCAA AGATGAAGGT GAGCGGTGTG 
GTGGCCTTCA AATGTGAGAA CGTGGCTACT CTCGACCCTG TGACTTTTGA GACGCCTGAG 
TCATTCATTA CCCTTGACAA GTGGTCCGCC AAAAAAGCCG GCTCCATCTC TTTCGACTTC 
CGCACCACTG AACCCAACGG CCTGCTCCTC TTCAGCCATG GAAAACCCAA ACCCCAGCAA 
CAGAAAGACC CTAAGAGCCC TAAAACATTG AAGGTGGATT TCTTTGCCAT TGAGATGCTG 
GACGGCCACC TCTACCTTCT TCTCGACATG GGCTCAGGCA CCACCAAAAC TAGAGCTGTC 
AACAAGAAAG TCAATGACGG AGAATGGTAC CACGTGGACT TCCAGAGAGA TGGAAGATCA 
GGCACCATTT CCGTCAACTC GATTCGCACT CCCTACAACG CTCCTGGTGA GAGTGAGATA 
CTGGACCTGG ACGATAAACT GTACCTGGGC GGCCTGCCAG AGGACCGGGC CGGTCTGATC 
TTCCCCACGG AGGTGTGGAC CGCTCTCCTA AATTACGGCT ATGTGGGCTG TGTGCGGGAT 
CTGTTCATGG ATGGCCAGAG CAAGGACATT CGGCGGATAG CAGAGGCTCA GAGAGCGGTC 
GGGGTGAAGC CGTCTTGCTC TAAAGAACCC CCCAAACAGT GTCTGAGCAA CCCCTGTCTG 
AACAGCGGCA CCTGCAGAGA GGGCTGGAAC CGCTATGTGT GCGACTGCTC CGGGACGGGA 
TATCTGGGAC GCTCCTGTGA GAGAGATGCC ACGATCCTCA GTTACGATGG TAGTAAGTTC 
ATGAAGATCC AGCTGCCGGT GGTGATGCAC ACAGAAGCGG AGGACGTGTC CCTGCGCTTT 
CGTTCTCAAC GGGCCTACGG TGTTCTAATG GCGACCACAT CGCAAAACTC TGCTGATACT 
CTGAGGCTGG AGCTGGACGG AGGGCGGGTC CGACTCACTG TCAATCTAGG TAAAGGTCCA 
GAGACCATAT TTTCGGGCCA GAACTTGAAT GACAACGAAT GGCACACAGT TCGTGTCGTC 
AGAAGAGGCA AAAGTCTAAA GCTGATGGTG GACGACTTGC AGCCCTCTGA AGGTCAAATC 
ACTGGGGACC ACACGCAGCT GGAGTTCCAC AATGTGGAAA CGGGCATCGT TACCGAGAAG 
CGCTACATGC CCGCTGTGCC CTCCAACTTC ATCGGACACC TGCAGGGTTT GTCCTTCAAT 
GGCATGTCCT ACATTGACTT GTGCAAGAAC GGCGATATCG ACTACTGCGA ACTGAACGCC 
ATGATCGGCT ACAGGAGCAT CGTGGCAGAC CCAGTGACCT TCAAGTCTCG CTCCAGCTAT 
GTGACTTTGC CCACCCTGCA GGCCTACTAT TCGATGCACC TCTTCTTCCA GTTTAAGACT 
ACTTCTCCAG ATGGGCTCAT CCTGTACAAC CGAGGAGATG GCAATGACTT TATAGTGGTA 
GAGCTGGTTA AAGGATATCT CCATTACGTG TCAGATCTGG GAAATGGAGC TCATCTCATC 
AAAGGCAATT CCAACACACC GCTAAATGAC AATCACTGGC ACAACGTGAT GATCTCGCGA 
GATACAAACA ACCTCCATAC TGTCAAAATA GACACCAAAA TCACCACACA GACTACCATG 
GGAGCCAAGA ACCTGGATCT GAAAGGTGAT CTGTATGTTG GAGGTGTGGC TAAAGATATG 
TATAAGGACT TGCCAAAGCT GGTGCATTCA AAAGAGGGCT TCCAGGGCTG CCTGGCATCT 
GTGGACCTGA ACGGCCGCTT GCCAGACCTG CAGTCAGACG CACTGTCCAC CGCCGGCCAG 
GTGGAGAGAG GCTGCGAAGG CCCCAGCACT ACCTGTCAGG AGGACTCCTG CTCCAATCAG 
GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT AGTTGCGACT GCAGCATGAC ATCATATGGA 
GGGCCACTCT GCAATGACCC GGGAACCACA TACATATTCG GACGTGATGG AGGGCTTATT 
GTGTACACAT GGCCACCCAA CGACAGACCG AGCACACGAG CTGACCGACT GGCTGTCGGT 
TTTAGTACCC AGCAGAAGGA TGCAGTGCTG GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA 
GACTACCTTC AGCTTCAGAT TGAAAGAGGC AATATCAAAG TGGTGTTCAA TGTGGGCACC 
GATGACATAA ACATTGAAGA AACTTCAAAG TTTGTAAATG ATGGAAAGTA CCACATAGTT 
CGCTTTACGA GAAGTGGTGG TAACGCCACC TTGCAGGTGG ATGATCTGCC AGTCATTGAG 
CGCTACCCAT CAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGGTGGGTT AGCTCACCCA 
CCACTTCCCG AGGCCAAGGG TTACCCGAGC CCAGAGGTGA TTCGGGAGTC CAGCAGCACC 
ACGGGCATGG TCGTAGGCAT CGTTGCAGCC GCTGCACTCT GCATCCTAAT CCTGCTCTAC 
GCCATGTACA AATACAGAAA CCGCGACGAA GGGTCTTACC ACGTGGACGA GAGTCGTAAC 
TACATTAGTA ATTCTGCCAC GCAGCCCAAT GGAGCTGCCG TCAAAGAAAA GCCCATCGGC 
GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335817.1
CDS	986..5239
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLGKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480142.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480142.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGGTTACA 
ATATCCGTGG ATGGGATTCT GACCACCACT GGATATACGC AAGAGGACTA CACCATGCTA 
GGCTCGGATG ACTTTTTCTA TGTCGGCGGT AGTCCCAGCA CTGCCGATCT TCCTGGATCA 
CCTGTCAGCA ACAACTTTAT GGGCTGCTTG AAAGAGGTTG TTTACAAAAA CAACGATGTA 
AGACTTGAGC TGTCTCGACT GGCCAAGCTG GGCGACCCAA AGATGAAGGT GAGCGGTGTG 
GTGGCCTTCA AATGTGAGAA CGTGGCTACT CTCGACCCTG TGACTTTTGA GACGCCTGAG 
TCATTCATTA CCCTTGACAA GTGGTCCGCC AAAAAAGCCG GCTCCATCTC TTTCGACTTC 
CGCACCACTG AACCCAACGG CCTGCTCCTC TTCAGCCATG GAAAACCCAA ACCCCAGCAA 
CAGAAAGACC CTAAGAGCCC TAAAACATTG AAGGTGGATT TCTTTGCCAT TGAGATGCTG 
GACGGCCACC TCTACCTTCT TCTCGACATG GGCTCAGGCA CCACCAAAAC TAGAGCTGTC 
AACAAGAAAG TCAATGACGG AGAATGGTAC CACGTGGACT TCCAGAGAGA TGGAAGATCA 
GGCACCATTT CCGTCAACTC GATTCGCACT CCCTACAACG CTCCTGGTGA GAGTGAGATA 
CTGGACCTGG ACGATAAACT GTACCTGGGC GGCCTGCCAG AGGACCGGGC CGGTCTGATC 
TTCCCCACGG AGGTGTGGAC CGCTCTCCTA AATTACGGCT ATGTGGGCTG TGTGCGGGAT 
CTGTTCATGG ATGGCCAGAG CAAGGACATT CGGCGGATAG CAGAGGCTCA GAGAGCGGTC 
GGGGTGAAGC CGTCTTGCTC TAAAGAACCC CCCAAACAGT GTCTGAGCAA CCCCTGTCTG 
AACAGCGGCA CCTGCAGAGA GGGCTGGAAC CGCTATGTGT GCGACTGCTC CGGGACGGGA 
TATCTGGGAC GCTCCTGTGA GAGAGATGCC ACGATCCTCA GTTACGATGG TAGTAAGTTC 
ATGAAGATCC AGCTGCCGGT GGTGATGCAC ACAGAAGCGG AGGACGTGTC CCTGCGCTTT 
CGTTCTCAAC GGGCCTACGG TGTTCTAATG GCGACCACAT CGCAAAACTC TGCTGATACT 
CTGAGGCTGG AGCTGGACGG AGGGCGGGTC CGACTCACTG TCAATCTAGG TAAAGGTCCA 
GAGACCATAT TTTCGGGCCA GAACTTGAAT GACAACGAAT GGCACACAGT TCGTGTCGTC 
AGAAGAGGCA AAAGTCTAAA GCTGATGGTG GACGACTTGC AGCCCTCTGA AGGTCAAATC 
ACTGGGGACC ACACGCAGCT GGAGTTCCAC AATGTGGAAA CGGGCATCGT TACCGAGAAG 
CGCTACATGC CCGCTGTGCC CTCCAACTTC ATCGGACACC TGCAGGGTTT GTCCTTCAAT 
GGCATGTCCT ACATTGACTT GTGCAAGAAC GGCGATATCG ACTACTGCGA ACTGAACGCC 
ATGATCGGCT ACAGGAGCAT CGTGGCAGAC CCAGTGACCT TCAAGTCTCG CTCCAGCTAT 
GTGACTTTGC CCACCCTGCA GGCCTACTAT TCGATGCACC TCTTCTTCCA GTTTAAGACT 
ACTTCTCCAG ATGGGCTCAT CCTGTACAAC CGAGGAGATG GCAATGACTT TATAGTGGTA 
GAGCTGGTTA AAGGATATCT CCATTACGTG TCAGATCTGG GAAATGGAGC TCATCTCATC 
AAAGGCAATT CCAACACACC GCTAAATGAC AATCACTGGC ACAACGTGAT GATCTCGCGA 
GATACAAACA ACCTCCATAC TGTCAAAATA GACACCAAAA TCACCACACA GACTACCATG 
GGAGCCAAGA ACCTGGATCT GAAAGGTGAT CTGTATGTTG GAGGTGTGGC TAAAGATATG 
TATAAGGACT TGCCAAAGCT GGTGCATTCA AAAGAGGGCT TCCAGGGCTG CCTGGCATCT 
GTGGACCTGA ACGGCCGCTT GCCAGACCTG CAGTCAGACG CACTGTCCAC CGCCGGCCAG 
GTGGAGAGAG GCTGCGAAGG CCCCAGCACT ACCTGTCAGG AGGACTCCTG CTCCAATCAG 
GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT AGTTGCGACT GCAGCATGAC ATCATATGGA 
GGGCCACTCT GCAATGACCC GGGAACCACA TACATATTCG GACGTGATGG AGGGCTTATT 
GTGTACACAT GGCCACCCAA CGACAGACCG AGCACACGAG CTGACCGACT GGCTGTCGGT 
TTTAGTACCC AGCAGAAGGA TGCAGTGCTG GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA 
GACTACCTTC AGCTTCAGAT TGAAAGAGGC AATATCAAAG TGGTGTTCAA TGTGGGCACC 
GATGACATAA ACATTGAAGA AACTTCAAAG TTTGTAAATG ATGGAAAGTA CCACATAGTT 
CGCTTTACGA GAAGTGGTGG TAACGCCACC TTGCAGGTGG ATGATCTGCC AGTCATTGAG 
CGCTACCCAT CAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGGTGGGTT AGCTCACCCA 
CCACTTCCCG AGGCCAAGGG TTACCCGAGC CCAGAGGTGA TTCGGGAGTC CAGCAGCACC 
ACGGGCATGG TCGTAGGCAT CGTTGCAGCC GCTGCACTCT GCATCCTAAT CCTGCTCTAC 
GCCATGTACA AATACAGAAA CCGCGACGAA GGGTCTTACC ACGTGGACGA GAGTCGTAAC 
TACATTAGTA ATTCTGCCAC GCAGCCCAAT GGAGCTGCCG TCAAAGAAAA GCCCATCGGC 
GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480143.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335818.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X22, mRNA.

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CloneID	ODa148216
Clone ID Related Accession (Same CDS sequence)	XM_021480143.1
Accession Version	XM_021480143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGGTCTTGCG 
CACCTGATGA TGGGCGACCA AGAGAGAGAA GAATATATGG CCACATTCAA GGGTTCGGAG 
TATTTCTGTT ACGATCTGTC CCCGAACCCC ATCCAGAGCA GTAGCGATGA GATTACGCTC 
TCCTTCAAGA CCCTGCAGCG CAATGGACTG ATGCTTCACA CCGGAAAGTC TGCAGACTAC 
GTCAACCTGG CGCTGAAGAA CGGAGCCGTC TCGCTGGTCA TCAACCTGGG CTCTGGGGCC 
TTCGAGGCCC TGGTGGAGCC CGTCAATGGG AAATTTAATG ACAATGCCTG GCATGATGTG 
AAGGTTACCA GGAACCTTCG TCAGCACTCA GGCATTGGAC ACGCTATGGT TACAATATCC 
GTGGATGGGA TTCTGACCAC CACTGGATAT ACGCAAGAGG ACTACACCAT GCTAGGCTCG 
GATGACTTTT TCTATGTCGG CGGTAGTCCC AGCACTGCCG ATCTTCCTGG ATCACCTGTC 
AGCAACAACT TTATGGGCTG CTTGAAAGAG GTTGTTTACA AAAACAACGA TGTAAGACTT 
GAGCTGTCTC GACTGGCCAA GCTGGGCGAC CCAAAGATGA AGGTGAGCGG TGTGGTGGCC 
TTCAAATGTG AGAACGTGGC TACTCTCGAC CCTGTGACTT TTGAGACGCC TGAGTCATTC 
ATTACCCTTG ACAAGTGGTC CGCCAAAAAA GCCGGCTCCA TCTCTTTCGA CTTCCGCACC 
ACTGAACCCA ACGGCCTGCT CCTCTTCAGC CATGGAAAAC CCAAACCCCA GCAACAGAAA 
GACCCTAAGA GCCCTAAAAC ATTGAAGGTG GATTTCTTTG CCATTGAGAT GCTGGACGGC 
CACCTCTACC TTCTTCTCGA CATGGGCTCA GGCACCACCA AAACTAGAGC TGTCAACAAG 
AAAGTCAATG ACGGAGAATG GTACCACGTG GACTTCCAGA GAGATGGAAG ATCAGGCACC 
ATTTCCGTCA ACTCGATTCG CACTCCCTAC AACGCTCCTG GTGAGAGTGA GATACTGGAC 
CTGGACGATA AACTGTACCT GGGCGGCCTG CCAGAGGACC GGGCCGGTCT GATCTTCCCC 
ACGGAGGTGT GGACCGCTCT CCTAAATTAC GGCTATGTGG GCTGTGTGCG GGATCTGTTC 
ATGGATGGCC AGAGCAAGGA CATTCGGCGG ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG 
AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA CAGTGTCTGA GCAACCCCTG TCTGAACAGC 
GGCACCTGCA GAGAGGGCTG GAACCGCTAT GTGTGCGACT GCTCCGGGAC GGGATATCTG 
GGACGCTCCT GTGAGAGAGA TGCCACGATC CTCAGTTACG ATGGTAGTAA GTTCATGAAG 
ATCCAGCTGC CGGTGGTGAT GCACACAGAA GCGGAGGACG TGTCCCTGCG CTTTCGTTCT 
CAACGGGCCT ACGGTGTTCT AATGGCGACC ACATCGCAAA ACTCTGCTGA TACTCTGAGG 
CTGGAGCTGG ACGGAGGGCG GGTCCGACTC ACTGTCAATC TAGACTGTAT CAGGATAAAC 
TGTACTACCA GTAAAGGTCC AGAGACCATA TTTTCGGGCC AGAACTTGAA TGACAACGAA 
TGGCACACAG TTCGTGTCGT CAGAAGAGGC AAAAGTCTAA AGCTGATGGT GGACGACTTG 
CAGCCCTCTG AAGGTCAAAT CACTGGGGAC CACACGCAGC TGGAGTTCCA CAATGTGGAA 
ACGGGCATCG TTACCGAGAA GCGCTACATG CCCGCTGTGC CCTCCAACTT CATCGGACAC 
CTGCAGGGTT TGTCCTTCAA TGGCATGTCC TACATTGACT TGTGCAAGAA CGGCGATATC 
GACTACTGCG AACTGAACGC CATGATCGGC TACAGGAGCA TCGTGGCAGA CCCAGTGACC 
TTCAAGTCTC GCTCCAGCTA TGTGACTTTG CCCACCCTGC AGGCCTACTA TTCGATGCAC 
CTCTTCTTCC AGTTTAAGAC TACTTCTCCA GATGGGCTCA TCCTGTACAA CCGAGGAGAT 
GGCAATGACT TTATAGTGGT AGAGCTGGTT AAAGGATATC TCCATTACGT GTCAGATCTG 
GGAAATGGAG CTCATCTCAT CAAAGGCAAT TCCAACACAC CGCTAAATGA CAATCACTGG 
CACAACGTGA TGATCTCGCG AGATACAAAC AACCTCCATA CTGTCAAAAT AGACACCAAA 
ATCACCACAC AGACTACCAT GGGAGCCAAG AACCTGGATC TGAAAGGTGA TCTGTATGTT 
GGAGGTGTGG CTAAAGATAT GTATAAGGAC TTGCCAAAGC TGGTGCATTC AAAAGAGGGC 
TTCCAGGGCT GCCTGGCATC TGTGGACCTG AACGGCCGCT TGCCAGACCT GCAGTCAGAC 
GCACTGTCCA CCGCCGGCCA GGTGGAGAGA GGCTGCGAAG GCCCCAGCAC TACCTGTCAG 
GAGGACTCCT GCTCCAATCA GGGCGTGTGT CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC 
TGCAGCATGA CATCATATGG AGGGCCACTC TGCAATGACC CGGGAACCAC ATACATATTC 
GGACGTGATG GAGGGCTTAT TGTGTACACA TGGCCACCCA ACGACAGACC GAGCACACGA 
GCTGACCGAC TGGCTGTCGG TTTTAGTACC CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG 
GACAGCTCTT CTGGCCTGGG AGACTACCTT CAGCTTCAGA TTGAAAGAGG CAATATCAAA 
GTGGTGTTCA ATGTGGGCAC CGATGACATA AACATTGAAG AAACTTCAAA GTTTGTAAAT 
GATGGAAAGT ACCACATAGT TCGCTTTACG AGAAGTGGTG GTAACGCCAC CTTGCAGGTG 
GATGATCTGC CAGTCATTGA GCGCTACCCA TCAGGCAACA TTGATAACGA ACGCCTGGCG 
ATTGCTAGAC AGCGAATCCC ATATCGGCTT GGTCGAGTAG TTGACGATTG GCTACTCGAC 
AAAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335818.1
CDS	985..5400
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEGLAHLMMGDQEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480143.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480143.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGGTCTTGCG 
CACCTGATGA TGGGCGACCA AGAGAGAGAA GAATATATGG CCACATTCAA GGGTTCGGAG 
TATTTCTGTT ACGATCTGTC CCCGAACCCC ATCCAGAGCA GTAGCGATGA GATTACGCTC 
TCCTTCAAGA CCCTGCAGCG CAATGGACTG ATGCTTCACA CCGGAAAGTC TGCAGACTAC 
GTCAACCTGG CGCTGAAGAA CGGAGCCGTC TCGCTGGTCA TCAACCTGGG CTCTGGGGCC 
TTCGAGGCCC TGGTGGAGCC CGTCAATGGG AAATTTAATG ACAATGCCTG GCATGATGTG 
AAGGTTACCA GGAACCTTCG TCAGCACTCA GGCATTGGAC ACGCTATGGT TACAATATCC 
GTGGATGGGA TTCTGACCAC CACTGGATAT ACGCAAGAGG ACTACACCAT GCTAGGCTCG 
GATGACTTTT TCTATGTCGG CGGTAGTCCC AGCACTGCCG ATCTTCCTGG ATCACCTGTC 
AGCAACAACT TTATGGGCTG CTTGAAAGAG GTTGTTTACA AAAACAACGA TGTAAGACTT 
GAGCTGTCTC GACTGGCCAA GCTGGGCGAC CCAAAGATGA AGGTGAGCGG TGTGGTGGCC 
TTCAAATGTG AGAACGTGGC TACTCTCGAC CCTGTGACTT TTGAGACGCC TGAGTCATTC 
ATTACCCTTG ACAAGTGGTC CGCCAAAAAA GCCGGCTCCA TCTCTTTCGA CTTCCGCACC 
ACTGAACCCA ACGGCCTGCT CCTCTTCAGC CATGGAAAAC CCAAACCCCA GCAACAGAAA 
GACCCTAAGA GCCCTAAAAC ATTGAAGGTG GATTTCTTTG CCATTGAGAT GCTGGACGGC 
CACCTCTACC TTCTTCTCGA CATGGGCTCA GGCACCACCA AAACTAGAGC TGTCAACAAG 
AAAGTCAATG ACGGAGAATG GTACCACGTG GACTTCCAGA GAGATGGAAG ATCAGGCACC 
ATTTCCGTCA ACTCGATTCG CACTCCCTAC AACGCTCCTG GTGAGAGTGA GATACTGGAC 
CTGGACGATA AACTGTACCT GGGCGGCCTG CCAGAGGACC GGGCCGGTCT GATCTTCCCC 
ACGGAGGTGT GGACCGCTCT CCTAAATTAC GGCTATGTGG GCTGTGTGCG GGATCTGTTC 
ATGGATGGCC AGAGCAAGGA CATTCGGCGG ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG 
AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA CAGTGTCTGA GCAACCCCTG TCTGAACAGC 
GGCACCTGCA GAGAGGGCTG GAACCGCTAT GTGTGCGACT GCTCCGGGAC GGGATATCTG 
GGACGCTCCT GTGAGAGAGA TGCCACGATC CTCAGTTACG ATGGTAGTAA GTTCATGAAG 
ATCCAGCTGC CGGTGGTGAT GCACACAGAA GCGGAGGACG TGTCCCTGCG CTTTCGTTCT 
CAACGGGCCT ACGGTGTTCT AATGGCGACC ACATCGCAAA ACTCTGCTGA TACTCTGAGG 
CTGGAGCTGG ACGGAGGGCG GGTCCGACTC ACTGTCAATC TAGACTGTAT CAGGATAAAC 
TGTACTACCA GTAAAGGTCC AGAGACCATA TTTTCGGGCC AGAACTTGAA TGACAACGAA 
TGGCACACAG TTCGTGTCGT CAGAAGAGGC AAAAGTCTAA AGCTGATGGT GGACGACTTG 
CAGCCCTCTG AAGGTCAAAT CACTGGGGAC CACACGCAGC TGGAGTTCCA CAATGTGGAA 
ACGGGCATCG TTACCGAGAA GCGCTACATG CCCGCTGTGC CCTCCAACTT CATCGGACAC 
CTGCAGGGTT TGTCCTTCAA TGGCATGTCC TACATTGACT TGTGCAAGAA CGGCGATATC 
GACTACTGCG AACTGAACGC CATGATCGGC TACAGGAGCA TCGTGGCAGA CCCAGTGACC 
TTCAAGTCTC GCTCCAGCTA TGTGACTTTG CCCACCCTGC AGGCCTACTA TTCGATGCAC 
CTCTTCTTCC AGTTTAAGAC TACTTCTCCA GATGGGCTCA TCCTGTACAA CCGAGGAGAT 
GGCAATGACT TTATAGTGGT AGAGCTGGTT AAAGGATATC TCCATTACGT GTCAGATCTG 
GGAAATGGAG CTCATCTCAT CAAAGGCAAT TCCAACACAC CGCTAAATGA CAATCACTGG 
CACAACGTGA TGATCTCGCG AGATACAAAC AACCTCCATA CTGTCAAAAT AGACACCAAA 
ATCACCACAC AGACTACCAT GGGAGCCAAG AACCTGGATC TGAAAGGTGA TCTGTATGTT 
GGAGGTGTGG CTAAAGATAT GTATAAGGAC TTGCCAAAGC TGGTGCATTC AAAAGAGGGC 
TTCCAGGGCT GCCTGGCATC TGTGGACCTG AACGGCCGCT TGCCAGACCT GCAGTCAGAC 
GCACTGTCCA CCGCCGGCCA GGTGGAGAGA GGCTGCGAAG GCCCCAGCAC TACCTGTCAG 
GAGGACTCCT GCTCCAATCA GGGCGTGTGT CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC 
TGCAGCATGA CATCATATGG AGGGCCACTC TGCAATGACC CGGGAACCAC ATACATATTC 
GGACGTGATG GAGGGCTTAT TGTGTACACA TGGCCACCCA ACGACAGACC GAGCACACGA 
GCTGACCGAC TGGCTGTCGG TTTTAGTACC CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG 
GACAGCTCTT CTGGCCTGGG AGACTACCTT CAGCTTCAGA TTGAAAGAGG CAATATCAAA 
GTGGTGTTCA ATGTGGGCAC CGATGACATA AACATTGAAG AAACTTCAAA GTTTGTAAAT 
GATGGAAAGT ACCACATAGT TCGCTTTACG AGAAGTGGTG GTAACGCCAC CTTGCAGGTG 
GATGATCTGC CAGTCATTGA GCGCTACCCA TCAGGCAACA TTGATAACGA ACGCCTGGCG 
ATTGCTAGAC AGCGAATCCC ATATCGGCTT GGTCGAGTAG TTGACGATTG GCTACTCGAC 
AAAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480144.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335819.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X23, mRNA.

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CloneID	ODa148217
Clone ID Related Accession (Same CDS sequence)	XM_021480144.1
Accession Version	XM_021480144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGCCCCAG CACTACCTGT 
CAGGAGGACT CCTGCTCCAA TCAGGGCGTG TGTCTGCAGC AGTGGGAGGG GTTTAGTTGC 
GACTGCAGCA TGACATCATA TGGAGGGCCA CTCTGCAATG ACCCGGGAAC CACATACATA 
TTCGGACGTG ATGGAGGGCT TATTGTGTAC ACATGGCCAC CCAACGACAG ACCGAGCACA 
CGAGCTGACC GACTGGCTGT CGGTTTTAGT ACCCAGCAGA AGGATGCAGT GCTGGTTAGG 
GTGGACAGCT CTTCTGGCCT GGGAGACTAC CTTCAGCTTC AGATTGAAAG AGGCAATATC 
AAAGTGGTGT TCAATGTGGG CACCGATGAC ATAAACATTG AAGAAACTTC AAAGTTTGTA 
AATGATGGAA AGTACCACAT AGTTCGCTTT ACGAGAAGTG GTGGTAACGC CACCTTGCAG 
GTGGATGATC TGCCAGTCAT TGAGCGCTAC CCATCAGGCA ACATTGATAA CGAACGCCTG 
GCGATTGCTA GACAGCGAAT CCCATATCGG CTTGGTCGAG TAGTTGACGA TTGGCTACTC 
GACAAAGGTC GGCAGCTCAC CATCTTCAAC AGTCAGACCA CCATAAAGAT TGGAGGCTGG 
GAGAAGGGCA GCCGGCCCTT CCAGGGTCAG CTCTCAGGGC TTTACTACAA TGGGCTAAAA 
GTACTCAACA TGGCCGCTGA GGGTGACCCT AATGTGCGTG TGGAGGGTAG TGCCCGGCTG 
GTAGGAGACA TGCCCTCCTC TTCTATCACG CCACAGTCCA GCGTCTCTGC TGCGGGAAAT 
CGTTCAGAAA CGTCACCCTC CATCACTGAC ATCACCACTA CAACGGCGTC CAACCGCCAG 
GGCAAGCAGA CCACAACTCC ACAGGACGAT CTTCTCGTAG CGTCTGCAGA ATGCCCCAGC 
GATGATGAGG ACATTGACCC TTGCGACCCA AGTTCAGGTG GGTTAGCTCA CCCACCACTT 
CCCGAGGCCA AGGGTTACCC GAGCCCAGAG GTGATTCGGG AGTCCAGCAG CACCACGGGC 
ATGGTCGTAG GCATCGTTGC AGCCGCTGCA CTCTGCATCC TAATCCTGCT CTACGCCATG 
TACAAATACA GAAACCGCGA CGAAGGGTCT TACCACGTGG ACGAGAGTCG TAACTACATT 
AGTAATTCTG CCACGCAGCC CAATGGAGCT GCCGTCAAAG AAAAGCCCAT CGGCGTGCCC 
AAAAATAAAA AAGATAAAAA GAACAAGGAC AAGGAGTACT ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335819.1
CDS	985..5352
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLGKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480144.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480144.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGCCCCAG CACTACCTGT 
CAGGAGGACT CCTGCTCCAA TCAGGGCGTG TGTCTGCAGC AGTGGGAGGG GTTTAGTTGC 
GACTGCAGCA TGACATCATA TGGAGGGCCA CTCTGCAATG ACCCGGGAAC CACATACATA 
TTCGGACGTG ATGGAGGGCT TATTGTGTAC ACATGGCCAC CCAACGACAG ACCGAGCACA 
CGAGCTGACC GACTGGCTGT CGGTTTTAGT ACCCAGCAGA AGGATGCAGT GCTGGTTAGG 
GTGGACAGCT CTTCTGGCCT GGGAGACTAC CTTCAGCTTC AGATTGAAAG AGGCAATATC 
AAAGTGGTGT TCAATGTGGG CACCGATGAC ATAAACATTG AAGAAACTTC AAAGTTTGTA 
AATGATGGAA AGTACCACAT AGTTCGCTTT ACGAGAAGTG GTGGTAACGC CACCTTGCAG 
GTGGATGATC TGCCAGTCAT TGAGCGCTAC CCATCAGGCA ACATTGATAA CGAACGCCTG 
GCGATTGCTA GACAGCGAAT CCCATATCGG CTTGGTCGAG TAGTTGACGA TTGGCTACTC 
GACAAAGGTC GGCAGCTCAC CATCTTCAAC AGTCAGACCA CCATAAAGAT TGGAGGCTGG 
GAGAAGGGCA GCCGGCCCTT CCAGGGTCAG CTCTCAGGGC TTTACTACAA TGGGCTAAAA 
GTACTCAACA TGGCCGCTGA GGGTGACCCT AATGTGCGTG TGGAGGGTAG TGCCCGGCTG 
GTAGGAGACA TGCCCTCCTC TTCTATCACG CCACAGTCCA GCGTCTCTGC TGCGGGAAAT 
CGTTCAGAAA CGTCACCCTC CATCACTGAC ATCACCACTA CAACGGCGTC CAACCGCCAG 
GGCAAGCAGA CCACAACTCC ACAGGACGAT CTTCTCGTAG CGTCTGCAGA ATGCCCCAGC 
GATGATGAGG ACATTGACCC TTGCGACCCA AGTTCAGGTG GGTTAGCTCA CCCACCACTT 
CCCGAGGCCA AGGGTTACCC GAGCCCAGAG GTGATTCGGG AGTCCAGCAG CACCACGGGC 
ATGGTCGTAG GCATCGTTGC AGCCGCTGCA CTCTGCATCC TAATCCTGCT CTACGCCATG 
TACAAATACA GAAACCGCGA CGAAGGGTCT TACCACGTGG ACGAGAGTCG TAACTACATT 
AGTAATTCTG CCACGCAGCC CAATGGAGCT GCCGTCAAAG AAAAGCCCAT CGGCGTGCCC 
AAAAATAAAA AAGATAAAAA GAACAAGGAC AAGGAGTACT ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480145.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335820.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X24, mRNA.

Price & Availability↑

CloneID	ODa148218
Clone ID Related Accession (Same CDS sequence)	XM_021480145.1
Accession Version	XM_021480145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGGTTACA 
ATATCCGTGG ATGGGATTCT GACCACCACT GGATATACGC AAGAGGACTA CACCATGCTA 
GGCTCGGATG ACTTTTTCTA TGTCGGCGGT AGTCCCAGCA CTGCCGATCT TCCTGGATCA 
CCTGTCAGCA ACAACTTTAT GGGCTGCTTG AAAGAGGTTG TTTACAAAAA CAACGATGTA 
AGACTTGAGC TGTCTCGACT GGCCAAGCTG GGCGACCCAA AGATGAAGGT GAGCGGTGTG 
GTGGCCTTCA AATGTGAGAA CGTGGCTACT CTCGACCCTG TGACTTTTGA GACGCCTGAG 
TCATTCATTA CCCTTGACAA GTGGTCCGCC AAAAAAGCCG GCTCCATCTC TTTCGACTTC 
CGCACCACTG AACCCAACGG CCTGCTCCTC TTCAGCCATG GAAAACCCAA ACCCCAGCAA 
CAGAAAGACC CTAAGAGCCC TAAAACATTG AAGGTGGATT TCTTTGCCAT TGAGATGCTG 
GACGGCCACC TCTACCTTCT TCTCGACATG GGCTCAGGCA CCACCAAAAC TAGAGCTGTC 
AACAAGAAAG TCAATGACGG AGAATGGTAC CACGTGGACT TCCAGAGAGA TGGAAGATCA 
GGCACCATTT CCGTCAACTC GATTCGCACT CCCTACAACG CTCCTGGTGA GAGTGAGATA 
CTGGACCTGG ACGATAAACT GTACCTGGGC GGCCTGCCAG AGGACCGGGC CGGTCTGATC 
TTCCCCACGG AGGTGTGGAC CGCTCTCCTA AATTACGGCT ATGTGGGCTG TGTGCGGGAT 
CTGTTCATGG ATGGCCAGAG CAAGGACATT CGGCGGATAG CAGAGGCTCA GAGAGCGGTC 
GGGGTGAAGC CGTCTTGCTC TAAAGAACCC CCCAAACAGT GTCTGAGCAA CCCCTGTCTG 
AACAGCGGCA CCTGCAGAGA GGGCTGGAAC CGCTATGTGT GCGACTGCTC CGGGACGGGA 
TATCTGGGAC GCTCCTGTGA GAGAGATGCC ACGATCCTCA GTTACGATGG TAGTAAGTTC 
ATGAAGATCC AGCTGCCGGT GGTGATGCAC ACAGAAGCGG AGGACGTGTC CCTGCGCTTT 
CGTTCTCAAC GGGCCTACGG TGTTCTAATG GCGACCACAT CGCAAAACTC TGCTGATACT 
CTGAGGCTGG AGCTGGACGG AGGGCGGGTC CGACTCACTG TCAATCTAGG TAAAGGTCCA 
GAGACCATAT TTTCGGGCCA GAACTTGAAT GACAACGAAT GGCACACAGT TCGTGTCGTC 
AGAAGAGGCA AAAGTCTAAA GCTGATGGTG GACGACTTGC AGCCCTCTGA AGGTCAAATC 
ACTGGGGACC ACACGCAGCT GGAGTTCCAC AATGTGGAAA CGGGCATCGT TACCGAGAAG 
CGCTACATGC CCGCTGTGCC CTCCAACTTC ATCGGACACC TGCAGGGTTT GTCCTTCAAT 
GGCATGTCCT ACATTGACTT GTGCAAGAAC GGCGATATCG ACTACTGCGA ACTGAACGCC 
ATGATCGGCT ACAGGAGCAT CGTGGCAGAC CCAGTGACCT TCAAGTCTCG CTCCAGCTAT 
GTGACTTTGC CCACCCTGCA GGCCTACTAT TCGATGCACC TCTTCTTCCA GTTTAAGACT 
ACTTCTCCAG ATGGGCTCAT CCTGTACAAC CGAGGAGATG GCAATGACTT TATAGTGGTA 
GAGCTGGTTA AAGGATATCT CCATTACGTG TCAGATCTGG GAAATGGAGC TCATCTCATC 
AAAGGCAATT CCAACACACC GCTAAATGAC AATCACTGGC ACAACGTGAT GATCTCGCGA 
GATACAAACA ACCTCCATAC TGTCAAAATA GACACCAAAA TCACCACACA GACTACCATG 
GGAGCCAAGA ACCTGGATCT GAAAGGTGAT CTGTATGTTG GAGGTGTGGC TAAAGATATG 
TATAAGGACT TGCCAAAGCT GGTGCATTCA AAAGAGGGCT TCCAGGGCTG CCTGGCATCT 
GTGGACCTGA ACGGCCGCTT GCCAGACCTG CAGTCAGACG CACTGTCCAC CGCCGGCCAG 
GTGGAGAGAG GCTGCGAAGG CCCCAGCACT ACCTGTCAGG AGGACTCCTG CTCCAATCAG 
GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT AGTTGCGACT GCAGCATGAC ATCATATGGA 
GGGCCACTCT GCAATGACCC GGGAACCACA TACATATTCG GACGTGATGG AGGGCTTATT 
GTGTACACAT GGCCACCCAA CGACAGACCG AGCACACGAG CTGACCGACT GGCTGTCGGT 
TTTAGTACCC AGCAGAAGGA TGCAGTGCTG GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA 
GACTACCTTC AGCTTCAGAT TGAAAGAGGC AATATCAAAG TGGTGTTCAA TGTGGGCACC 
GATGACATAA ACATTGAAGA AACTTCAAAG TTTGTAAATG ATGGAAAGTA CCACATAGTT 
CGCTTTACGA GAAGTGGTGG TAACGCCACC TTGCAGGTGG ATGATCTGCC AGTCATTGAG 
CGCTACCCAT CAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335820.1
CDS	986..5230
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLGKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480145.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480145.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGGTTACA 
ATATCCGTGG ATGGGATTCT GACCACCACT GGATATACGC AAGAGGACTA CACCATGCTA 
GGCTCGGATG ACTTTTTCTA TGTCGGCGGT AGTCCCAGCA CTGCCGATCT TCCTGGATCA 
CCTGTCAGCA ACAACTTTAT GGGCTGCTTG AAAGAGGTTG TTTACAAAAA CAACGATGTA 
AGACTTGAGC TGTCTCGACT GGCCAAGCTG GGCGACCCAA AGATGAAGGT GAGCGGTGTG 
GTGGCCTTCA AATGTGAGAA CGTGGCTACT CTCGACCCTG TGACTTTTGA GACGCCTGAG 
TCATTCATTA CCCTTGACAA GTGGTCCGCC AAAAAAGCCG GCTCCATCTC TTTCGACTTC 
CGCACCACTG AACCCAACGG CCTGCTCCTC TTCAGCCATG GAAAACCCAA ACCCCAGCAA 
CAGAAAGACC CTAAGAGCCC TAAAACATTG AAGGTGGATT TCTTTGCCAT TGAGATGCTG 
GACGGCCACC TCTACCTTCT TCTCGACATG GGCTCAGGCA CCACCAAAAC TAGAGCTGTC 
AACAAGAAAG TCAATGACGG AGAATGGTAC CACGTGGACT TCCAGAGAGA TGGAAGATCA 
GGCACCATTT CCGTCAACTC GATTCGCACT CCCTACAACG CTCCTGGTGA GAGTGAGATA 
CTGGACCTGG ACGATAAACT GTACCTGGGC GGCCTGCCAG AGGACCGGGC CGGTCTGATC 
TTCCCCACGG AGGTGTGGAC CGCTCTCCTA AATTACGGCT ATGTGGGCTG TGTGCGGGAT 
CTGTTCATGG ATGGCCAGAG CAAGGACATT CGGCGGATAG CAGAGGCTCA GAGAGCGGTC 
GGGGTGAAGC CGTCTTGCTC TAAAGAACCC CCCAAACAGT GTCTGAGCAA CCCCTGTCTG 
AACAGCGGCA CCTGCAGAGA GGGCTGGAAC CGCTATGTGT GCGACTGCTC CGGGACGGGA 
TATCTGGGAC GCTCCTGTGA GAGAGATGCC ACGATCCTCA GTTACGATGG TAGTAAGTTC 
ATGAAGATCC AGCTGCCGGT GGTGATGCAC ACAGAAGCGG AGGACGTGTC CCTGCGCTTT 
CGTTCTCAAC GGGCCTACGG TGTTCTAATG GCGACCACAT CGCAAAACTC TGCTGATACT 
CTGAGGCTGG AGCTGGACGG AGGGCGGGTC CGACTCACTG TCAATCTAGG TAAAGGTCCA 
GAGACCATAT TTTCGGGCCA GAACTTGAAT GACAACGAAT GGCACACAGT TCGTGTCGTC 
AGAAGAGGCA AAAGTCTAAA GCTGATGGTG GACGACTTGC AGCCCTCTGA AGGTCAAATC 
ACTGGGGACC ACACGCAGCT GGAGTTCCAC AATGTGGAAA CGGGCATCGT TACCGAGAAG 
CGCTACATGC CCGCTGTGCC CTCCAACTTC ATCGGACACC TGCAGGGTTT GTCCTTCAAT 
GGCATGTCCT ACATTGACTT GTGCAAGAAC GGCGATATCG ACTACTGCGA ACTGAACGCC 
ATGATCGGCT ACAGGAGCAT CGTGGCAGAC CCAGTGACCT TCAAGTCTCG CTCCAGCTAT 
GTGACTTTGC CCACCCTGCA GGCCTACTAT TCGATGCACC TCTTCTTCCA GTTTAAGACT 
ACTTCTCCAG ATGGGCTCAT CCTGTACAAC CGAGGAGATG GCAATGACTT TATAGTGGTA 
GAGCTGGTTA AAGGATATCT CCATTACGTG TCAGATCTGG GAAATGGAGC TCATCTCATC 
AAAGGCAATT CCAACACACC GCTAAATGAC AATCACTGGC ACAACGTGAT GATCTCGCGA 
GATACAAACA ACCTCCATAC TGTCAAAATA GACACCAAAA TCACCACACA GACTACCATG 
GGAGCCAAGA ACCTGGATCT GAAAGGTGAT CTGTATGTTG GAGGTGTGGC TAAAGATATG 
TATAAGGACT TGCCAAAGCT GGTGCATTCA AAAGAGGGCT TCCAGGGCTG CCTGGCATCT 
GTGGACCTGA ACGGCCGCTT GCCAGACCTG CAGTCAGACG CACTGTCCAC CGCCGGCCAG 
GTGGAGAGAG GCTGCGAAGG CCCCAGCACT ACCTGTCAGG AGGACTCCTG CTCCAATCAG 
GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT AGTTGCGACT GCAGCATGAC ATCATATGGA 
GGGCCACTCT GCAATGACCC GGGAACCACA TACATATTCG GACGTGATGG AGGGCTTATT 
GTGTACACAT GGCCACCCAA CGACAGACCG AGCACACGAG CTGACCGACT GGCTGTCGGT 
TTTAGTACCC AGCAGAAGGA TGCAGTGCTG GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA 
GACTACCTTC AGCTTCAGAT TGAAAGAGGC AATATCAAAG TGGTGTTCAA TGTGGGCACC 
GATGACATAA ACATTGAAGA AACTTCAAAG TTTGTAAATG ATGGAAAGTA CCACATAGTT 
CGCTTTACGA GAAGTGGTGG TAACGCCACC TTGCAGGTGG ATGATCTGCC AGTCATTGAG 
CGCTACCCAT CAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_021480146.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335821.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X25, mRNA.

Price & Availability↑

CloneID	ODa148219
Clone ID Related Accession (Same CDS sequence)	XM_021480146.1
Accession Version	XM_021480146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGCCCCAG CACTACCTGT 
CAGGAGGACT CCTGCTCCAA TCAGGGCGTG TGTCTGCAGC AGTGGGAGGG GTTTAGTTGC 
GACTGCAGCA TGACATCATA TGGAGGGCCA CTCTGCAATG ACCCGGGAAC CACATACATA 
TTCGGACGTG ATGGAGGGCT TATTGTGTAC ACATGGCCAC CCAACGACAG ACCGAGCACA 
CGAGCTGACC GACTGGCTGT CGGTTTTAGT ACCCAGCAGA AGGATGCAGT GCTGGTTAGG 
GTGGACAGCT CTTCTGGCCT GGGAGACTAC CTTCAGCTTC AGATTGAAAG AGGCAATATC 
AAAGTGGTGT TCAATGTGGG CACCGATGAC ATAAACATTG AAGAAACTTC AAAGTTTGTA 
AATGATGGAA AGTACCACAT AGTTCGCTTT ACGAGAAGTG GTGGTAACGC CACCTTGCAG 
GTGGATGATC TGCCAGTCAT TGAGCGCTAC CCATCAGGTC GGCAGCTCAC CATCTTCAAC 
AGTCAGACCA CCATAAAGAT TGGAGGCTGG GAGAAGGGCA GCCGGCCCTT CCAGGGTCAG 
CTCTCAGGGC TTTACTACAA TGGGCTAAAA GTACTCAACA TGGCCGCTGA GGGTGACCCT 
AATGTGCGTG TGGAGGGTAG TGCCCGGCTG GTAGGAGACA TGCCCTCCTC TTCTATCACG 
CCACAGTCCA GCGTCTCTGC TGCGGGAAAT CGTTCAGAAA CGTCACCCTC CATCACTGAC 
ATCACCACTA CAACGGCGTC CAACCGCCAG GGCAAGCAGA CCACAACTCC ACAGGACGAT 
CTTCTCGTAG CGTCTGCAGA ATGCCCCAGC GATGATGAGG ACATTGACCC TTGCGACCCA 
AGTTCAGCTC ACCCACCACT TCCCGAGGCC AAGGGTTACC CGAGCCCAGA GGTGATTCGG 
GAGTCCAGCA GCACCACGGG CATGGTCGTA GGCATCGTTG CAGCCGCTGC ACTCTGCATC 
CTAATCCTGC TCTACGCCAT GTACAAATAC AGAAACCGCG ACGAAGGGTC TTACCACGTG 
GACGAGAGTC GTAACTACAT TAGTAATTCT GCCACGCAGC CCAATGGAGC TGCCGTCAAA 
GAAAAGCCCA TCGGCGTGCC CAAAAATAAA AAAGATAAAA AGAACAAGGA CAAGGAGTAC 
TACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335821.1
CDS	986..5254
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLGKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_021480146.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480146.1

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGCCCCAG CACTACCTGT 
CAGGAGGACT CCTGCTCCAA TCAGGGCGTG TGTCTGCAGC AGTGGGAGGG GTTTAGTTGC 
GACTGCAGCA TGACATCATA TGGAGGGCCA CTCTGCAATG ACCCGGGAAC CACATACATA 
TTCGGACGTG ATGGAGGGCT TATTGTGTAC ACATGGCCAC CCAACGACAG ACCGAGCACA 
CGAGCTGACC GACTGGCTGT CGGTTTTAGT ACCCAGCAGA AGGATGCAGT GCTGGTTAGG 
GTGGACAGCT CTTCTGGCCT GGGAGACTAC CTTCAGCTTC AGATTGAAAG AGGCAATATC 
AAAGTGGTGT TCAATGTGGG CACCGATGAC ATAAACATTG AAGAAACTTC AAAGTTTGTA 
AATGATGGAA AGTACCACAT AGTTCGCTTT ACGAGAAGTG GTGGTAACGC CACCTTGCAG 
GTGGATGATC TGCCAGTCAT TGAGCGCTAC CCATCAGGTC GGCAGCTCAC CATCTTCAAC 
AGTCAGACCA CCATAAAGAT TGGAGGCTGG GAGAAGGGCA GCCGGCCCTT CCAGGGTCAG 
CTCTCAGGGC TTTACTACAA TGGGCTAAAA GTACTCAACA TGGCCGCTGA GGGTGACCCT 
AATGTGCGTG TGGAGGGTAG TGCCCGGCTG GTAGGAGACA TGCCCTCCTC TTCTATCACG 
CCACAGTCCA GCGTCTCTGC TGCGGGAAAT CGTTCAGAAA CGTCACCCTC CATCACTGAC 
ATCACCACTA CAACGGCGTC CAACCGCCAG GGCAAGCAGA CCACAACTCC ACAGGACGAT 
CTTCTCGTAG CGTCTGCAGA ATGCCCCAGC GATGATGAGG ACATTGACCC TTGCGACCCA 
AGTTCAGCTC ACCCACCACT TCCCGAGGCC AAGGGTTACC CGAGCCCAGA GGTGATTCGG 
GAGTCCAGCA GCACCACGGG CATGGTCGTA GGCATCGTTG CAGCCGCTGC ACTCTGCATC 
CTAATCCTGC TCTACGCCAT GTACAAATAC AGAAACCGCG ACGAAGGGTC TTACCACGTG 
GACGAGAGTC GTAACTACAT TAGTAATTCT GCCACGCAGC CCAATGGAGC TGCCGTCAAA 
GAAAAGCCCA TCGGCGTGCC CAAAAATAAA AAAGATAAAA AGAACAAGGA CAAGGAGTAC 
TACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306655.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304930.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X16, mRNA.

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CloneID	ODa44667
Clone ID Related Accession (Same CDS sequence)	XM_009306655.2
Accession Version	XM_009306655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGAG GGAGGGATAA CGAAGCATGC GTCCATTCAA CCAGATCTGT TACAATATCC 
GTGGATGGGA TTCTGACCAC CACTGGATAT ACGCAAGAGG ACTACACCAT GCTAGGCTCG 
GATGACTTTT TCTATGTCGG CGGTAGTCCC AGCACTGCCG ATCTTCCTGG ATCACCTGTC 
AGCAACAACT TTATGGGCTG CTTGAAAGAG GTTGTTTACA AAAACAACGA TGTAAGACTT 
GAGCTGTCTC GACTGGCCAA GCTGGGCGAC CCAAAGATGA AGGTGAGCGG TGTGGTGGCC 
TTCAAATGTG AGAACGTGGC TACTCTCGAC CCTGTGACTT TTGAGACGCC TGAGTCATTC 
ATTACCCTTG ACAAGTGGTC CGCCAAAAAA GCCGGCTCCA TCTCTTTCGA CTTCCGCACC 
ACTGAACCCA ACGGCCTGCT CCTCTTCAGC CATGGAAAAC CCAAACCCCA GCAACAGAAA 
GACCCTAAGA GCCCTAAAAC ATTGAAGGTG GATTTCTTTG CCATTGAGAT GCTGGACGGC 
CACCTCTACC TTCTTCTCGA CATGGGCTCA GGCACCACCA AAACTAGAGC TGTCAACAAG 
AAAGTCAATG ACGGAGAATG GTACCACGTG GACTTCCAGA GAGATGGAAG ATCAGGCACC 
ATTTCCGTCA ACTCGATTCG CACTCCCTAC AACGCTCCTG GTGAGAGTGA GATACTGGAC 
CTGGACGATA AACTGTACCT GGGCGGCCTG CCAGAGGACC GGGCCGGTCT GATCTTCCCC 
ACGGAGGTGT GGACCGCTCT CCTAAATTAC GGCTATGTGG GCTGTGTGCG GGATCTGTTC 
ATGGATGGCC AGAGCAAGGA CATTCGGCGG ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG 
AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA CAGTGTCTGA GCAACCCCTG TCTGAACAGC 
GGCACCTGCA GAGAGGGCTG GAACCGCTAT GTGTGCGACT GCTCCGGGAC GGGATATCTG 
GGACGCTCCT GTGAGAGAGA TGCCACGATC CTCAGTTACG ATGGTAGTAA GTTCATGAAG 
ATCCAGCTGC CGGTGGTGAT GCACACAGAA GCGGAGGACG TGTCCCTGCG CTTTCGTTCT 
CAACGGGCCT ACGGTGTTCT AATGGCGACC ACATCGCAAA ACTCTGCTGA TACTCTGAGG 
CTGGAGCTGG ACGGAGGGCG GGTCCGACTC ACTGTCAATC TAGACTGTAT CAGGATAAAC 
TGTACTACCA GTAAAGGTCC AGAGACCATA TTTTCGGGCC AGAACTTGAA TGACAACGAA 
TGGCACACAG TTCGTGTCGT CAGAAGAGGC AAAAGTCTAA AGCTGATGGT GGACGACTTG 
CAGCCCTCTG AAGGTCAAAT CACTGGGGAC CACACGCAGC TGGAGTTCCA CAATGTGGAA 
ACGGGCATCG TTACCGAGAA GCGCTACATG CCCGCTGTGC CCTCCAACTT CATCGGACAC 
CTGCAGGGTT TGTCCTTCAA TGGCATGTCC TACATTGACT TGTGCAAGAA CGGCGATATC 
GACTACTGCG AACTGAACGC CATGATCGGC TACAGGAGCA TCGTGGCAGA CCCAGTGACC 
TTCAAGTCTC GCTCCAGCTA TGTGACTTTG CCCACCCTGC AGGCCTACTA TTCGATGCAC 
CTCTTCTTCC AGTTTAAGAC TACTTCTCCA GATGGGCTCA TCCTGTACAA CCGAGGAGAT 
GGCAATGACT TTATAGTGGT AGAGCTGGTT AAAGGATATC TCCATTACGT GTCAGATCTG 
GGAAATGGAG CTCATCTCAT CAAAGGCAAT TCCAACACAC CGCTAAATGA CAATCACTGG 
CACAACGTGA TGATCTCGCG AGATACAAAC AACCTCCATA CTGTCAAAAT AGACACCAAA 
ATCACCACAC AGACTACCAT GGGAGCCAAG AACCTGGATC TGAAAGGTGA TCTGTATGTT 
GGAGGTGTGG CTAAAGATAT GTATAAGGAC TTGCCAAAGC TGGTGCATTC AAAAGAGGGC 
TTCCAGGGCT GCCTGGCATC TGTGGACCTG AACGGCCGCT TGCCAGACCT GCAGTCAGAC 
GCACTGTCCA CCGCCGGCCA GGTGGAGAGA GGCTGCGAAG GTGAGGAAAA CGTTGCATTG 
CTGAAAGCTG ACTTGCAAGG CCCCAGCACT ACCTGTCAGG AGGACTCCTG CTCCAATCAG 
GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT AGTTGCGACT GCAGCATGAC ATCATATGGA 
GGGCCACTCT GCAATGACCC GGGAACCACA TACATATTCG GACGTGATGG AGGGCTTATT 
GTGTACACAT GGCCACCCAA CGACAGACCG AGCACACGAG CTGACCGACT GGCTGTCGGT 
TTTAGTACCC AGCAGAAGGA TGCAGTGCTG GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA 
GACTACCTTC AGCTTCAGAT TGAAAGAGGC AATATCAAAG TGGTGTTCAA TGTGGGCACC 
GATGACATAA ACATTGAAGA AACTTCAAAG TTTGTAAATG ATGGAAAGTA CCACATAGTT 
CGCTTTACGA GAAGTGGTGG TAACGCCACC TTGCAGGTGG ATGATCTGCC AGTCATTGAG 
CGCTACCCAT CAGGCAACAT TGATAACGAA CGCCTGGCGA TTGCTAGACA GCGAATCCCA 
TATCGGCTTG GTCGAGTAGT TGACGATTGG CTACTCGACA AAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGGTGGGTT AGCTCACCCA CCACTTCCCG AGGCCAAGGG TTACCCGAGC 
CCAGAGGTGA TTCGGGAGTC CAGCAGCACC ACGGGCATGG TCGTAGGCAT CGTTGCAGCC 
GCTGCACTCT GCATCCTAAT CCTGCTCTAC GCCATGTACA AATACAGAAA CCGCGACGAA 
GGGTCTTACC ACGTGGACGA GAGTCGTAAC TACATTAGTA ATTCTGCCAC GCAGCCCAAT 
GGAGCTGCCG TCAAAGAAAA GCCCATCGGC GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC 
AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304930.1
CDS	633..4076
Translation	MPRGRDNEACVHSTRSVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306655.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306655.2

ORF Insert Sequence:

ATGCCGAGAG GGAGGGATAA CGAAGCATGC GTCCATTCAA CCAGATCTGT TACAATATCC 
GTGGATGGGA TTCTGACCAC CACTGGATAT ACGCAAGAGG ACTACACCAT GCTAGGCTCG 
GATGACTTTT TCTATGTCGG CGGTAGTCCC AGCACTGCCG ATCTTCCTGG ATCACCTGTC 
AGCAACAACT TTATGGGCTG CTTGAAAGAG GTTGTTTACA AAAACAACGA TGTAAGACTT 
GAGCTGTCTC GACTGGCCAA GCTGGGCGAC CCAAAGATGA AGGTGAGCGG TGTGGTGGCC 
TTCAAATGTG AGAACGTGGC TACTCTCGAC CCTGTGACTT TTGAGACGCC TGAGTCATTC 
ATTACCCTTG ACAAGTGGTC CGCCAAAAAA GCCGGCTCCA TCTCTTTCGA CTTCCGCACC 
ACTGAACCCA ACGGCCTGCT CCTCTTCAGC CATGGAAAAC CCAAACCCCA GCAACAGAAA 
GACCCTAAGA GCCCTAAAAC ATTGAAGGTG GATTTCTTTG CCATTGAGAT GCTGGACGGC 
CACCTCTACC TTCTTCTCGA CATGGGCTCA GGCACCACCA AAACTAGAGC TGTCAACAAG 
AAAGTCAATG ACGGAGAATG GTACCACGTG GACTTCCAGA GAGATGGAAG ATCAGGCACC 
ATTTCCGTCA ACTCGATTCG CACTCCCTAC AACGCTCCTG GTGAGAGTGA GATACTGGAC 
CTGGACGATA AACTGTACCT GGGCGGCCTG CCAGAGGACC GGGCCGGTCT GATCTTCCCC 
ACGGAGGTGT GGACCGCTCT CCTAAATTAC GGCTATGTGG GCTGTGTGCG GGATCTGTTC 
ATGGATGGCC AGAGCAAGGA CATTCGGCGG ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG 
AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA CAGTGTCTGA GCAACCCCTG TCTGAACAGC 
GGCACCTGCA GAGAGGGCTG GAACCGCTAT GTGTGCGACT GCTCCGGGAC GGGATATCTG 
GGACGCTCCT GTGAGAGAGA TGCCACGATC CTCAGTTACG ATGGTAGTAA GTTCATGAAG 
ATCCAGCTGC CGGTGGTGAT GCACACAGAA GCGGAGGACG TGTCCCTGCG CTTTCGTTCT 
CAACGGGCCT ACGGTGTTCT AATGGCGACC ACATCGCAAA ACTCTGCTGA TACTCTGAGG 
CTGGAGCTGG ACGGAGGGCG GGTCCGACTC ACTGTCAATC TAGACTGTAT CAGGATAAAC 
TGTACTACCA GTAAAGGTCC AGAGACCATA TTTTCGGGCC AGAACTTGAA TGACAACGAA 
TGGCACACAG TTCGTGTCGT CAGAAGAGGC AAAAGTCTAA AGCTGATGGT GGACGACTTG 
CAGCCCTCTG AAGGTCAAAT CACTGGGGAC CACACGCAGC TGGAGTTCCA CAATGTGGAA 
ACGGGCATCG TTACCGAGAA GCGCTACATG CCCGCTGTGC CCTCCAACTT CATCGGACAC 
CTGCAGGGTT TGTCCTTCAA TGGCATGTCC TACATTGACT TGTGCAAGAA CGGCGATATC 
GACTACTGCG AACTGAACGC CATGATCGGC TACAGGAGCA TCGTGGCAGA CCCAGTGACC 
TTCAAGTCTC GCTCCAGCTA TGTGACTTTG CCCACCCTGC AGGCCTACTA TTCGATGCAC 
CTCTTCTTCC AGTTTAAGAC TACTTCTCCA GATGGGCTCA TCCTGTACAA CCGAGGAGAT 
GGCAATGACT TTATAGTGGT AGAGCTGGTT AAAGGATATC TCCATTACGT GTCAGATCTG 
GGAAATGGAG CTCATCTCAT CAAAGGCAAT TCCAACACAC CGCTAAATGA CAATCACTGG 
CACAACGTGA TGATCTCGCG AGATACAAAC AACCTCCATA CTGTCAAAAT AGACACCAAA 
ATCACCACAC AGACTACCAT GGGAGCCAAG AACCTGGATC TGAAAGGTGA TCTGTATGTT 
GGAGGTGTGG CTAAAGATAT GTATAAGGAC TTGCCAAAGC TGGTGCATTC AAAAGAGGGC 
TTCCAGGGCT GCCTGGCATC TGTGGACCTG AACGGCCGCT TGCCAGACCT GCAGTCAGAC 
GCACTGTCCA CCGCCGGCCA GGTGGAGAGA GGCTGCGAAG GTGAGGAAAA CGTTGCATTG 
CTGAAAGCTG ACTTGCAAGG CCCCAGCACT ACCTGTCAGG AGGACTCCTG CTCCAATCAG 
GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT AGTTGCGACT GCAGCATGAC ATCATATGGA 
GGGCCACTCT GCAATGACCC GGGAACCACA TACATATTCG GACGTGATGG AGGGCTTATT 
GTGTACACAT GGCCACCCAA CGACAGACCG AGCACACGAG CTGACCGACT GGCTGTCGGT 
TTTAGTACCC AGCAGAAGGA TGCAGTGCTG GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA 
GACTACCTTC AGCTTCAGAT TGAAAGAGGC AATATCAAAG TGGTGTTCAA TGTGGGCACC 
GATGACATAA ACATTGAAGA AACTTCAAAG TTTGTAAATG ATGGAAAGTA CCACATAGTT 
CGCTTTACGA GAAGTGGTGG TAACGCCACC TTGCAGGTGG ATGATCTGCC AGTCATTGAG 
CGCTACCCAT CAGGCAACAT TGATAACGAA CGCCTGGCGA TTGCTAGACA GCGAATCCCA 
TATCGGCTTG GTCGAGTAGT TGACGATTGG CTACTCGACA AAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGGTGGGTT AGCTCACCCA CCACTTCCCG AGGCCAAGGG TTACCCGAGC 
CCAGAGGTGA TTCGGGAGTC CAGCAGCACC ACGGGCATGG TCGTAGGCAT CGTTGCAGCC 
GCTGCACTCT GCATCCTAAT CCTGCTCTAC GCCATGTACA AATACAGAAA CCGCGACGAA 
GGGTCTTACC ACGTGGACGA GAGTCGTAAC TACATTAGTA ATTCTGCCAC GCAGCCCAAT 
GGAGCTGCCG TCAAAGAAAA GCCCATCGGC GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC 
AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( NM_001080021.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073490.1 Danio rerio neurexin 1a (nrxn1a), transcript variant 1, mRNA.

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CloneID	ODa44658
Clone ID Related Accession (Same CDS sequence)	NM_001080021.2
Accession Version	NM_001080021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL928656.4, BX255907.8 and CR933760.7. On or before Nov 9, 2016 this sequence version replaced XM_009306647.2, NM_001080021.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ641424.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA4476737, SAMEA4476739 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT AAACAAACTA CATTGTTCGG TTACAATATC CGTGGATGGG 
ATTCTGACCA CCACTGGATA TACGCAAGAG GACTACACCA TGCTAGGCTC GGATGACTTT 
TTCTATGTCG GCGGTAGTCC CAGCACTGCC GATCTTCCTG GATCACCTGT CAGCAACAAC 
TTTATGGGCT GCTTGAAAGA GGTTGTTTAC AAAAACAACG ATGTAAGACT TGAGCTGTCT 
CGACTGGCCA AGCTGGGCGA CCCAAAGATG AAGGTGAGCG GTGTGGTGGC CTTCAAATGT 
GAGAACGTGG CTACTCTCGA CCCTGTGACT TTTGAGACGC CTGAGTCATT CATTACCCTT 
GACAAGTGGT CCGCCAAAAA AGCCGGCTCC ATCTCTTTCG ACTTCCGCAC CACTGAACCC 
AACGGCCTGC TCCTCTTCAG CCATGGAAAA CCCAAACCCC AGCAACAGAA AGACCCTAAG 
AGCCCTAAAA CATTGAAGGT GGATTTCTTT GCCATTGAGA TGCTGGACGG CCACCTCTAC 
CTTCTTCTCG ACATGGGCTC AGGCACCACC AAAACTAGAG CTGTCAACAA GAAAGTCAAT 
GACGGAGAAT GGTACCACGT GGACTTCCAG AGAGATGGAA GATCAGGCAC CATTTCCGTC 
AACTCGATTC GCACTCCCTA CAACGCTCCT GGTGAGAGTG AGATACTGGA CCTGGACGAT 
AAACTGTACC TGGGCGGCCT GCCAGAGGAC CGGGCCGGTC TGATCTTCCC CACGGAGGTG 
TGGACCGCTC TCCTAAATTA CGGCTATGTG GGCTGTGTGC GGGATCTGTT CATGGATGGC 
CAGAGCAAGG ACATTCGGCG GATAGCAGAG GCTCAGAGAG CGGTCGGGGT GAAGCCGTCT 
TGCTCTAAAG AACCCCCCAA ACAGTGTCTG AGCAACCCCT GTCTGAACAG CGGCACCTGC 
AGAGAGGGCT GGAACCGCTA TGTGTGCGAC TGCTCCGGGA CGGGATATCT GGGACGCTCC 
TGTGAGAGAG ATGCCACGAT CCTCAGTTAC GATGGTAGTA AGTTCATGAA GATCCAGCTG 
CCGGTGGTGA TGCACACAGA AGCGGAGGAC GTGTCCCTGC GCTTTCGTTC TCAACGGGCC 
TACGGTGTTC TAATGGCGAC CACATCGCAA AACTCTGCTG ATACTCTGAG GCTGGAGCTG 
GACGGAGGGC GGGTCCGACT CACTGTCAAT CTAGACTGTA TCAGGATAAA CTGTACTACC 
AGTAAAGGTC CAGAGACCAT ATTTTCGGGC CAGAACTTGA ATGACAACGA ATGGCACACA 
GTTCGTGTCG TCAGAAGAGG CAAAAGTCTA AAGCTGATGG TGGACGACTT GCAGCCCTCT 
GAAGGTCAAA TCACTGGGGA CCACACGCAG CTGGAGTTCC ACAATGTGGA AACGGGCATC 
GTTACCGAGA AGCGCTACAT GCCCGCTGTG CCCTCCAACT TCATCGGACA CCTGCAGGGT 
TTGTCCTTCA ATGGCATGTC CTACATTGAC TTGTGCAAGA ACGGCGATAT CGACTACTGC 
GAACTGAACG CCATGATCGG CTACAGGAGC ATCGTGGCAG ACCCAGTGAC CTTCAAGTCT 
CGCTCCAGCT ATGTGACTTT GCCCACCCTG CAGGCCTACT ATTCGATGCA CCTCTTCTTC 
CAGTTTAAGA CTACTTCTCC AGATGGGCTC ATCCTGTACA ACCGAGGAGA TGGCAATGAC 
TTTATAGTGG TAGAGCTGGT TAAAGGATAT CTCCATTACG TGTCAGATCT GGGAAATGGA 
GCTCATCTCA TCAAAGGCAA TTCCAACACA CCGCTAAATG ACAATCACTG GCACAACGTG 
ATGATCTCGC GAGATACAAA CAACCTCCAT ACTGTCAAAA TAGACACCAA AATCACCACA 
CAGACTACCA TGGGAGCCAA GAACCTGGAT CTGAAAGGTG ATCTGTATGT TGGAGGTGTG 
GCTAAAGATA TGTATAAGGA CTTGCCAAAG CTGGTGCATT CAAAAGAGGG CTTCCAGGGC 
TGCCTGGCAT CTGTGGACCT GAACGGCCGC TTGCCAGACC TGCAGTCAGA CGCACTGTCC 
ACCGCCGGCC AGGTGGAGAG AGGCTGCGAA GGCCCCAGCA CTACCTGTCA GGAGGACTCC 
TGCTCCAATC AGGGCGTGTG TCTGCAGCAG TGGGAGGGGT TTAGTTGCGA CTGCAGCATG 
ACATCATATG GAGGGCCACT CTGCAATGAC CCGGGAACCA CATACATATT CGGACGTGAT 
GGAGGGCTTA TTGTGTACAC ATGGCCACCC AACGACAGAC CGAGCACACG AGCTGACCGA 
CTGGCTGTCG GTTTTAGTAC CCAGCAGAAG GATGCAGTGC TGGTTAGGGT GGACAGCTCT 
TCTGGCCTGG GAGACTACCT TCAGCTTCAG ATTGAAAGAG GCAATATCAA AGTGGTGTTC 
AATGTGGGCA CCGATGACAT AAACATTGAA GAAACTTCAA AGTTTGTAAA TGATGGAAAG 
TACCACATAG TTCGCTTTAC GAGAAGTGGT GGTAACGCCA CCTTGCAGGT GGATGATCTG 
CCAGTCATTG AGCGCTACCC ATCAGGCAAC ATTGATAACG AACGCCTGGC GATTGCTAGA 
CAGCGAATCC CATATCGGCT TGGTCGAGTA GTTGACGATT GGCTACTCGA CAAAGGTCGG 
CAGCTCACCA TCTTCAACAG TCAGACCACC ATAAAGATTG GAGGCTGGGA GAAGGGCAGC 
CGGCCCTTCC AGGGTCAGCT CTCAGGGCTT TACTACAATG GGCTAAAAGT ACTCAACATG 
GCCGCTGAGG GTGACCCTAA TGTGCGTGTG GAGGGTAGTG CCCGGCTGGT AGGAGACATG 
CCCTCCTCTT CTATCACGCC ACAGTCCAGC GTCTCTGCTG CGGGAAATCG TTCAGAAACG 
TCACCCTCCA TCACTGACAT CACCACTACA ACGGCGTCCA ACCGCCAGGG CAAGCAGACC 
ACAACTCCAC AGGACGATCT TCTCGTAGCG TCTGCAGAAT GCCCCAGCGA TGATGAGGAC 
ATTGACCCTT GCGACCCAAG TTCAGGTGGG TTAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073490.1
CDS	983..5458
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVNKLHCSVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	NM_001080021.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080021.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT AAACAAACTA CATTGTTCGG TTACAATATC CGTGGATGGG 
ATTCTGACCA CCACTGGATA TACGCAAGAG GACTACACCA TGCTAGGCTC GGATGACTTT 
TTCTATGTCG GCGGTAGTCC CAGCACTGCC GATCTTCCTG GATCACCTGT CAGCAACAAC 
TTTATGGGCT GCTTGAAAGA GGTTGTTTAC AAAAACAACG ATGTAAGACT TGAGCTGTCT 
CGACTGGCCA AGCTGGGCGA CCCAAAGATG AAGGTGAGCG GTGTGGTGGC CTTCAAATGT 
GAGAACGTGG CTACTCTCGA CCCTGTGACT TTTGAGACGC CTGAGTCATT CATTACCCTT 
GACAAGTGGT CCGCCAAAAA AGCCGGCTCC ATCTCTTTCG ACTTCCGCAC CACTGAACCC 
AACGGCCTGC TCCTCTTCAG CCATGGAAAA CCCAAACCCC AGCAACAGAA AGACCCTAAG 
AGCCCTAAAA CATTGAAGGT GGATTTCTTT GCCATTGAGA TGCTGGACGG CCACCTCTAC 
CTTCTTCTCG ACATGGGCTC AGGCACCACC AAAACTAGAG CTGTCAACAA GAAAGTCAAT 
GACGGAGAAT GGTACCACGT GGACTTCCAG AGAGATGGAA GATCAGGCAC CATTTCCGTC 
AACTCGATTC GCACTCCCTA CAACGCTCCT GGTGAGAGTG AGATACTGGA CCTGGACGAT 
AAACTGTACC TGGGCGGCCT GCCAGAGGAC CGGGCCGGTC TGATCTTCCC CACGGAGGTG 
TGGACCGCTC TCCTAAATTA CGGCTATGTG GGCTGTGTGC GGGATCTGTT CATGGATGGC 
CAGAGCAAGG ACATTCGGCG GATAGCAGAG GCTCAGAGAG CGGTCGGGGT GAAGCCGTCT 
TGCTCTAAAG AACCCCCCAA ACAGTGTCTG AGCAACCCCT GTCTGAACAG CGGCACCTGC 
AGAGAGGGCT GGAACCGCTA TGTGTGCGAC TGCTCCGGGA CGGGATATCT GGGACGCTCC 
TGTGAGAGAG ATGCCACGAT CCTCAGTTAC GATGGTAGTA AGTTCATGAA GATCCAGCTG 
CCGGTGGTGA TGCACACAGA AGCGGAGGAC GTGTCCCTGC GCTTTCGTTC TCAACGGGCC 
TACGGTGTTC TAATGGCGAC CACATCGCAA AACTCTGCTG ATACTCTGAG GCTGGAGCTG 
GACGGAGGGC GGGTCCGACT CACTGTCAAT CTAGACTGTA TCAGGATAAA CTGTACTACC 
AGTAAAGGTC CAGAGACCAT ATTTTCGGGC CAGAACTTGA ATGACAACGA ATGGCACACA 
GTTCGTGTCG TCAGAAGAGG CAAAAGTCTA AAGCTGATGG TGGACGACTT GCAGCCCTCT 
GAAGGTCAAA TCACTGGGGA CCACACGCAG CTGGAGTTCC ACAATGTGGA AACGGGCATC 
GTTACCGAGA AGCGCTACAT GCCCGCTGTG CCCTCCAACT TCATCGGACA CCTGCAGGGT 
TTGTCCTTCA ATGGCATGTC CTACATTGAC TTGTGCAAGA ACGGCGATAT CGACTACTGC 
GAACTGAACG CCATGATCGG CTACAGGAGC ATCGTGGCAG ACCCAGTGAC CTTCAAGTCT 
CGCTCCAGCT ATGTGACTTT GCCCACCCTG CAGGCCTACT ATTCGATGCA CCTCTTCTTC 
CAGTTTAAGA CTACTTCTCC AGATGGGCTC ATCCTGTACA ACCGAGGAGA TGGCAATGAC 
TTTATAGTGG TAGAGCTGGT TAAAGGATAT CTCCATTACG TGTCAGATCT GGGAAATGGA 
GCTCATCTCA TCAAAGGCAA TTCCAACACA CCGCTAAATG ACAATCACTG GCACAACGTG 
ATGATCTCGC GAGATACAAA CAACCTCCAT ACTGTCAAAA TAGACACCAA AATCACCACA 
CAGACTACCA TGGGAGCCAA GAACCTGGAT CTGAAAGGTG ATCTGTATGT TGGAGGTGTG 
GCTAAAGATA TGTATAAGGA CTTGCCAAAG CTGGTGCATT CAAAAGAGGG CTTCCAGGGC 
TGCCTGGCAT CTGTGGACCT GAACGGCCGC TTGCCAGACC TGCAGTCAGA CGCACTGTCC 
ACCGCCGGCC AGGTGGAGAG AGGCTGCGAA GGCCCCAGCA CTACCTGTCA GGAGGACTCC 
TGCTCCAATC AGGGCGTGTG TCTGCAGCAG TGGGAGGGGT TTAGTTGCGA CTGCAGCATG 
ACATCATATG GAGGGCCACT CTGCAATGAC CCGGGAACCA CATACATATT CGGACGTGAT 
GGAGGGCTTA TTGTGTACAC ATGGCCACCC AACGACAGAC CGAGCACACG AGCTGACCGA 
CTGGCTGTCG GTTTTAGTAC CCAGCAGAAG GATGCAGTGC TGGTTAGGGT GGACAGCTCT 
TCTGGCCTGG GAGACTACCT TCAGCTTCAG ATTGAAAGAG GCAATATCAA AGTGGTGTTC 
AATGTGGGCA CCGATGACAT AAACATTGAA GAAACTTCAA AGTTTGTAAA TGATGGAAAG 
TACCACATAG TTCGCTTTAC GAGAAGTGGT GGTAACGCCA CCTTGCAGGT GGATGATCTG 
CCAGTCATTG AGCGCTACCC ATCAGGCAAC ATTGATAACG AACGCCTGGC GATTGCTAGA 
CAGCGAATCC CATATCGGCT TGGTCGAGTA GTTGACGATT GGCTACTCGA CAAAGGTCGG 
CAGCTCACCA TCTTCAACAG TCAGACCACC ATAAAGATTG GAGGCTGGGA GAAGGGCAGC 
CGGCCCTTCC AGGGTCAGCT CTCAGGGCTT TACTACAATG GGCTAAAAGT ACTCAACATG 
GCCGCTGAGG GTGACCCTAA TGTGCGTGTG GAGGGTAGTG CCCGGCTGGT AGGAGACATG 
CCCTCCTCTT CTATCACGCC ACAGTCCAGC GTCTCTGCTG CGGGAAATCG TTCAGAAACG 
TCACCCTCCA TCACTGACAT CACCACTACA ACGGCGTCCA ACCGCCAGGG CAAGCAGACC 
ACAACTCCAC AGGACGATCT TCTCGTAGCG TCTGCAGAAT GCCCCAGCGA TGATGAGGAC 
ATTGACCCTT GCGACCCAAG TTCAGGTGGG TTAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306641.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304916.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44652
Clone ID Related Accession (Same CDS sequence)	XM_009306641.2 , XM_009306641.3
Accession Version	XM_009306641.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGGTGGGTT AGCTCACCCA 
CCACTTCCCG AGGCCAAGGG TTACCCGAGC CCAGAGGTGA TTCGGGAGTC CAGCAGCACC 
ACGGGCATGG TCGTAGGCAT CGTTGCAGCC GCTGCACTCT GCATCCTAAT CCTGCTCTAC 
GCCATGTACA AATACAGAAA CCGCGACGAA GGGTCTTACC ACGTGGACGA GAGTCGTAAC 
TACATTAGTA ATTCTGCCAC GCAGCCCAAT GGAGCTGCCG TCAAAGAAAA GCCCATCGGC 
GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304916.1
CDS	983..5476
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306641.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306641.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGGTGGGTT AGCTCACCCA 
CCACTTCCCG AGGCCAAGGG TTACCCGAGC CCAGAGGTGA TTCGGGAGTC CAGCAGCACC 
ACGGGCATGG TCGTAGGCAT CGTTGCAGCC GCTGCACTCT GCATCCTAAT CCTGCTCTAC 
GCCATGTACA AATACAGAAA CCGCGACGAA GGGTCTTACC ACGTGGACGA GAGTCGTAAC 
TACATTAGTA ATTCTGCCAC GCAGCCCAAT GGAGCTGCCG TCAAAGAAAA GCCCATCGGC 
GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306641.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304916.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X1, mRNA.

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CloneID	ODa44652
Clone ID Related Accession (Same CDS sequence)	XM_009306641.2 , XM_009306641.3
Accession Version	XM_009306641.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306641.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGGTGGGTT AGCTCACCCA 
CCACTTCCCG AGGCCAAGGG TTACCCGAGC CCAGAGGTGA TTCGGGAGTC CAGCAGCACC 
ACGGGCATGG TCGTAGGCAT CGTTGCAGCC GCTGCACTCT GCATCCTAAT CCTGCTCTAC 
GCCATGTACA AATACAGAAA CCGCGACGAA GGGTCTTACC ACGTGGACGA GAGTCGTAAC 
TACATTAGTA ATTCTGCCAC GCAGCCCAAT GGAGCTGCCG TCAAAGAAAA GCCCATCGGC 
GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304916.1
CDS	986..5479
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306641.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306641.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGGTGGGTT AGCTCACCCA 
CCACTTCCCG AGGCCAAGGG TTACCCGAGC CCAGAGGTGA TTCGGGAGTC CAGCAGCACC 
ACGGGCATGG TCGTAGGCAT CGTTGCAGCC GCTGCACTCT GCATCCTAAT CCTGCTCTAC 
GCCATGTACA AATACAGAAA CCGCGACGAA GGGTCTTACC ACGTGGACGA GAGTCGTAAC 
TACATTAGTA ATTCTGCCAC GCAGCCCAAT GGAGCTGCCG TCAAAGAAAA GCCCATCGGC 
GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306642.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304917.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X2, mRNA.

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CloneID	ODa44653
Clone ID Related Accession (Same CDS sequence)	XM_009306642.3 , XM_009306642.2
Accession Version	XM_009306642.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306642.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGGCCTAT 
GTAGGAGGTC TTGCGCACCT GATGATGGGC GACCAAGGGA AAAGAAAAGA GAGAGAAGAA 
TATATGGCCA CATTCAAGGG TTCGGAGTAT TTCTGTTACG ATCTGTCCCC GAACCCCATC 
CAGAGCAGTA GCGATGAGAT TACGCTCTCC TTCAAGACCC TGCAGCGCAA TGGACTGATG 
CTTCACACCG GAAAGTCTGC AGACTACGTC AACCTGGCGC TGAAGAACGG AGCCGTCTCG 
CTGGTCATCA ACCTGGGCTC TGGGGCCTTC GAGGCCCTGG TGGAGCCCGT CAATGGGAAA 
TTTAATGACA ATGCCTGGCA TGATGTGAAG GTTACCAGGA ACCTTCGTCA GCACTCAGGC 
ATTGGACACG CTATGGTTAC AATATCCGTG GATGGGATTC TGACCACCAC TGGATATACG 
CAAGAGGACT ACACCATGCT AGGCTCGGAT GACTTTTTCT ATGTCGGCGG TAGTCCCAGC 
ACTGCCGATC TTCCTGGATC ACCTGTCAGC AACAACTTTA TGGGCTGCTT GAAAGAGGTT 
GTTTACAAAA ACAACGATGT AAGACTTGAG CTGTCTCGAC TGGCCAAGCT GGGCGACCCA 
AAGATGAAGG TGAGCGGTGT GGTGGCCTTC AAATGTGAGA ACGTGGCTAC TCTCGACCCT 
GTGACTTTTG AGACGCCTGA GTCATTCATT ACCCTTGACA AGTGGTCCGC CAAAAAAGCC 
GGCTCCATCT CTTTCGACTT CCGCACCACT GAACCCAACG GCCTGCTCCT CTTCAGCCAT 
GGAAAACCCA AACCCCAGCA ACAGAAAGAC CCTAAGAGCC CTAAAACATT GAAGGTGGAT 
TTCTTTGCCA TTGAGATGCT GGACGGCCAC CTCTACCTTC TTCTCGACAT GGGCTCAGGC 
ACCACCAAAA CTAGAGCTGT CAACAAGAAA GTCAATGACG GAGAATGGTA CCACGTGGAC 
TTCCAGAGAG ATGGAAGATC AGGCACCATT TCCGTCAACT CGATTCGCAC TCCCTACAAC 
GCTCCTGGTG AGAGTGAGAT ACTGGACCTG GACGATAAAC TGTACCTGGG CGGCCTGCCA 
GAGGACCGGG CCGGTCTGAT CTTCCCCACG GAGGTGTGGA CCGCTCTCCT AAATTACGGC 
TATGTGGGCT GTGTGCGGGA TCTGTTCATG GATGGCCAGA GCAAGGACAT TCGGCGGATA 
GCAGAGGCTC AGAGAGCGGT CGGGGTGAAG CCGTCTTGCT CTAAAGAACC CCCCAAACAG 
TGTCTGAGCA ACCCCTGTCT GAACAGCGGC ACCTGCAGAG AGGGCTGGAA CCGCTATGTG 
TGCGACTGCT CCGGGACGGG ATATCTGGGA CGCTCCTGTG AGAGAGATGC CACGATCCTC 
AGTTACGATG GTAGTAAGTT CATGAAGATC CAGCTGCCGG TGGTGATGCA CACAGAAGCG 
GAGGACGTGT CCCTGCGCTT TCGTTCTCAA CGGGCCTACG GTGTTCTAAT GGCGACCACA 
TCGCAAAACT CTGCTGATAC TCTGAGGCTG GAGCTGGACG GAGGGCGGGT CCGACTCACT 
GTCAATCTAG ACTGTATCAG GATAAACTGT ACTACCAGTA AAGGTCCAGA GACCATATTT 
TCGGGCCAGA ACTTGAATGA CAACGAATGG CACACAGTTC GTGTCGTCAG AAGAGGCAAA 
AGTCTAAAGC TGATGGTGGA CGACTTGCAG CCCTCTGAAG GTCAAATCAC TGGGGACCAC 
ACGCAGCTGG AGTTCCACAA TGTGGAAACG GGCATCGTTA CCGAGAAGCG CTACATGCCC 
GCTGTGCCCT CCAACTTCAT CGGACACCTG CAGGGTTTGT CCTTCAATGG CATGTCCTAC 
ATTGACTTGT GCAAGAACGG CGATATCGAC TACTGCGAAC TGAACGCCAT GATCGGCTAC 
AGGAGCATCG TGGCAGACCC AGTGACCTTC AAGTCTCGCT CCAGCTATGT GACTTTGCCC 
ACCCTGCAGG CCTACTATTC GATGCACCTC TTCTTCCAGT TTAAGACTAC TTCTCCAGAT 
GGGCTCATCC TGTACAACCG AGGAGATGGC AATGACTTTA TAGTGGTAGA GCTGGTTAAA 
GGATATCTCC ATTACGTGTC AGATCTGGGA AATGGAGCTC ATCTCATCAA AGGCAATTCC 
AACACACCGC TAAATGACAA TCACTGGCAC AACGTGATGA TCTCGCGAGA TACAAACAAC 
CTCCATACTG TCAAAATAGA CACCAAAATC ACCACACAGA CTACCATGGG AGCCAAGAAC 
CTGGATCTGA AAGGTGATCT GTATGTTGGA GGTGTGGCTA AAGATATGTA TAAGGACTTG 
CCAAAGCTGG TGCATTCAAA AGAGGGCTTC CAGGGCTGCC TGGCATCTGT GGACCTGAAC 
GGCCGCTTGC CAGACCTGCA GTCAGACGCA CTGTCCACCG CCGGCCAGGT GGAGAGAGGC 
TGCGAAGGTG AGGAAAACGT TGCATTGCTG AAAGCTGACT TGCAAGGCCC CAGCACTACC 
TGTCAGGAGG ACTCCTGCTC CAATCAGGGC GTGTGTCTGC AGCAGTGGGA GGGGTTTAGT 
TGCGACTGCA GCATGACATC ATATGGAGGG CCACTCTGCA ATGACCCGGG AACCACATAC 
ATATTCGGAC GTGATGGAGG GCTTATTGTG TACACATGGC CACCCAACGA CAGACCGAGC 
ACACGAGCTG ACCGACTGGC TGTCGGTTTT AGTACCCAGC AGAAGGATGC AGTGCTGGTT 
AGGGTGGACA GCTCTTCTGG CCTGGGAGAC TACCTTCAGC TTCAGATTGA AAGAGGCAAT 
ATCAAAGTGG TGTTCAATGT GGGCACCGAT GACATAAACA TTGAAGAAAC TTCAAAGTTT 
GTAAATGATG GAAAGTACCA CATAGTTCGC TTTACGAGAA GTGGTGGTAA CGCCACCTTG 
CAGGTGGATG ATCTGCCAGT CATTGAGCGC TACCCATCAG GCAACATTGA TAACGAACGC 
CTGGCGATTG CTAGACAGCG AATCCCATAT CGGCTTGGTC GAGTAGTTGA CGATTGGCTA 
CTCGACAAAG GTCGGCAGCT CACCATCTTC AACAGTCAGA CCACCATAAA GATTGGAGGC 
TGGGAGAAGG GCAGCCGGCC CTTCCAGGGT CAGCTCTCAG GGCTTTACTA CAATGGGCTA 
AAAGTACTCA ACATGGCCGC TGAGGGTGAC CCTAATGTGC GTGTGGAGGG TAGTGCCCGG 
CTGGTAGGAG ACATGCCCTC CTCTTCTATC ACGCCACAGT CCAGCGTCTC TGCTGCGGGA 
AATCGTTCAG AAACGTCACC CTCCATCACT GACATCACCA CTACAACGGC GTCCAACCGC 
CAGGGCAAGC AGACCACAAC TCCACAGGAC GATCTTCTCG TAGCGTCTGC AGAATGCCCC 
AGCGATGATG AGGACATTGA CCCTTGCGAC CCAAGTTCAG GTGGGTTAGC TCACCCACCA 
CTTCCCGAGG CCAAGGGTTA CCCGAGCCCA GAGGTGATTC GGGAGTCCAG CAGCACCACG 
GGCATGGTCG TAGGCATCGT TGCAGCCGCT GCACTCTGCA TCCTAATCCT GCTCTACGCC 
ATGTACAAAT ACAGAAACCG CGACGAAGGG TCTTACCACG TGGACGAGAG TCGTAACTAC 
ATTAGTAATT CTGCCACGCA GCCCAATGGA GCTGCCGTCA AAGAAAAGCC CATCGGCGTG 
CCCAAAAATA AAAAAGATAA AAAGAACAAG GACAAGGAGT ACTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304917.1
CDS	986..5476
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306642.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306642.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGGCCTAT 
GTAGGAGGTC TTGCGCACCT GATGATGGGC GACCAAGGGA AAAGAAAAGA GAGAGAAGAA 
TATATGGCCA CATTCAAGGG TTCGGAGTAT TTCTGTTACG ATCTGTCCCC GAACCCCATC 
CAGAGCAGTA GCGATGAGAT TACGCTCTCC TTCAAGACCC TGCAGCGCAA TGGACTGATG 
CTTCACACCG GAAAGTCTGC AGACTACGTC AACCTGGCGC TGAAGAACGG AGCCGTCTCG 
CTGGTCATCA ACCTGGGCTC TGGGGCCTTC GAGGCCCTGG TGGAGCCCGT CAATGGGAAA 
TTTAATGACA ATGCCTGGCA TGATGTGAAG GTTACCAGGA ACCTTCGTCA GCACTCAGGC 
ATTGGACACG CTATGGTTAC AATATCCGTG GATGGGATTC TGACCACCAC TGGATATACG 
CAAGAGGACT ACACCATGCT AGGCTCGGAT GACTTTTTCT ATGTCGGCGG TAGTCCCAGC 
ACTGCCGATC TTCCTGGATC ACCTGTCAGC AACAACTTTA TGGGCTGCTT GAAAGAGGTT 
GTTTACAAAA ACAACGATGT AAGACTTGAG CTGTCTCGAC TGGCCAAGCT GGGCGACCCA 
AAGATGAAGG TGAGCGGTGT GGTGGCCTTC AAATGTGAGA ACGTGGCTAC TCTCGACCCT 
GTGACTTTTG AGACGCCTGA GTCATTCATT ACCCTTGACA AGTGGTCCGC CAAAAAAGCC 
GGCTCCATCT CTTTCGACTT CCGCACCACT GAACCCAACG GCCTGCTCCT CTTCAGCCAT 
GGAAAACCCA AACCCCAGCA ACAGAAAGAC CCTAAGAGCC CTAAAACATT GAAGGTGGAT 
TTCTTTGCCA TTGAGATGCT GGACGGCCAC CTCTACCTTC TTCTCGACAT GGGCTCAGGC 
ACCACCAAAA CTAGAGCTGT CAACAAGAAA GTCAATGACG GAGAATGGTA CCACGTGGAC 
TTCCAGAGAG ATGGAAGATC AGGCACCATT TCCGTCAACT CGATTCGCAC TCCCTACAAC 
GCTCCTGGTG AGAGTGAGAT ACTGGACCTG GACGATAAAC TGTACCTGGG CGGCCTGCCA 
GAGGACCGGG CCGGTCTGAT CTTCCCCACG GAGGTGTGGA CCGCTCTCCT AAATTACGGC 
TATGTGGGCT GTGTGCGGGA TCTGTTCATG GATGGCCAGA GCAAGGACAT TCGGCGGATA 
GCAGAGGCTC AGAGAGCGGT CGGGGTGAAG CCGTCTTGCT CTAAAGAACC CCCCAAACAG 
TGTCTGAGCA ACCCCTGTCT GAACAGCGGC ACCTGCAGAG AGGGCTGGAA CCGCTATGTG 
TGCGACTGCT CCGGGACGGG ATATCTGGGA CGCTCCTGTG AGAGAGATGC CACGATCCTC 
AGTTACGATG GTAGTAAGTT CATGAAGATC CAGCTGCCGG TGGTGATGCA CACAGAAGCG 
GAGGACGTGT CCCTGCGCTT TCGTTCTCAA CGGGCCTACG GTGTTCTAAT GGCGACCACA 
TCGCAAAACT CTGCTGATAC TCTGAGGCTG GAGCTGGACG GAGGGCGGGT CCGACTCACT 
GTCAATCTAG ACTGTATCAG GATAAACTGT ACTACCAGTA AAGGTCCAGA GACCATATTT 
TCGGGCCAGA ACTTGAATGA CAACGAATGG CACACAGTTC GTGTCGTCAG AAGAGGCAAA 
AGTCTAAAGC TGATGGTGGA CGACTTGCAG CCCTCTGAAG GTCAAATCAC TGGGGACCAC 
ACGCAGCTGG AGTTCCACAA TGTGGAAACG GGCATCGTTA CCGAGAAGCG CTACATGCCC 
GCTGTGCCCT CCAACTTCAT CGGACACCTG CAGGGTTTGT CCTTCAATGG CATGTCCTAC 
ATTGACTTGT GCAAGAACGG CGATATCGAC TACTGCGAAC TGAACGCCAT GATCGGCTAC 
AGGAGCATCG TGGCAGACCC AGTGACCTTC AAGTCTCGCT CCAGCTATGT GACTTTGCCC 
ACCCTGCAGG CCTACTATTC GATGCACCTC TTCTTCCAGT TTAAGACTAC TTCTCCAGAT 
GGGCTCATCC TGTACAACCG AGGAGATGGC AATGACTTTA TAGTGGTAGA GCTGGTTAAA 
GGATATCTCC ATTACGTGTC AGATCTGGGA AATGGAGCTC ATCTCATCAA AGGCAATTCC 
AACACACCGC TAAATGACAA TCACTGGCAC AACGTGATGA TCTCGCGAGA TACAAACAAC 
CTCCATACTG TCAAAATAGA CACCAAAATC ACCACACAGA CTACCATGGG AGCCAAGAAC 
CTGGATCTGA AAGGTGATCT GTATGTTGGA GGTGTGGCTA AAGATATGTA TAAGGACTTG 
CCAAAGCTGG TGCATTCAAA AGAGGGCTTC CAGGGCTGCC TGGCATCTGT GGACCTGAAC 
GGCCGCTTGC CAGACCTGCA GTCAGACGCA CTGTCCACCG CCGGCCAGGT GGAGAGAGGC 
TGCGAAGGTG AGGAAAACGT TGCATTGCTG AAAGCTGACT TGCAAGGCCC CAGCACTACC 
TGTCAGGAGG ACTCCTGCTC CAATCAGGGC GTGTGTCTGC AGCAGTGGGA GGGGTTTAGT 
TGCGACTGCA GCATGACATC ATATGGAGGG CCACTCTGCA ATGACCCGGG AACCACATAC 
ATATTCGGAC GTGATGGAGG GCTTATTGTG TACACATGGC CACCCAACGA CAGACCGAGC 
ACACGAGCTG ACCGACTGGC TGTCGGTTTT AGTACCCAGC AGAAGGATGC AGTGCTGGTT 
AGGGTGGACA GCTCTTCTGG CCTGGGAGAC TACCTTCAGC TTCAGATTGA AAGAGGCAAT 
ATCAAAGTGG TGTTCAATGT GGGCACCGAT GACATAAACA TTGAAGAAAC TTCAAAGTTT 
GTAAATGATG GAAAGTACCA CATAGTTCGC TTTACGAGAA GTGGTGGTAA CGCCACCTTG 
CAGGTGGATG ATCTGCCAGT CATTGAGCGC TACCCATCAG GCAACATTGA TAACGAACGC 
CTGGCGATTG CTAGACAGCG AATCCCATAT CGGCTTGGTC GAGTAGTTGA CGATTGGCTA 
CTCGACAAAG GTCGGCAGCT CACCATCTTC AACAGTCAGA CCACCATAAA GATTGGAGGC 
TGGGAGAAGG GCAGCCGGCC CTTCCAGGGT CAGCTCTCAG GGCTTTACTA CAATGGGCTA 
AAAGTACTCA ACATGGCCGC TGAGGGTGAC CCTAATGTGC GTGTGGAGGG TAGTGCCCGG 
CTGGTAGGAG ACATGCCCTC CTCTTCTATC ACGCCACAGT CCAGCGTCTC TGCTGCGGGA 
AATCGTTCAG AAACGTCACC CTCCATCACT GACATCACCA CTACAACGGC GTCCAACCGC 
CAGGGCAAGC AGACCACAAC TCCACAGGAC GATCTTCTCG TAGCGTCTGC AGAATGCCCC 
AGCGATGATG AGGACATTGA CCCTTGCGAC CCAAGTTCAG GTGGGTTAGC TCACCCACCA 
CTTCCCGAGG CCAAGGGTTA CCCGAGCCCA GAGGTGATTC GGGAGTCCAG CAGCACCACG 
GGCATGGTCG TAGGCATCGT TGCAGCCGCT GCACTCTGCA TCCTAATCCT GCTCTACGCC 
ATGTACAAAT ACAGAAACCG CGACGAAGGG TCTTACCACG TGGACGAGAG TCGTAACTAC 
ATTAGTAATT CTGCCACGCA GCCCAATGGA GCTGCCGTCA AAGAAAAGCC CATCGGCGTG 
CCCAAAAATA AAAAAGATAA AAAGAACAAG GACAAGGAGT ACTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306642.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304917.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44653
Clone ID Related Accession (Same CDS sequence)	XM_009306642.3 , XM_009306642.2
Accession Version	XM_009306642.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGGCCTAT 
GTAGGAGGTC TTGCGCACCT GATGATGGGC GACCAAGGGA AAAGAAAAGA GAGAGAAGAA 
TATATGGCCA CATTCAAGGG TTCGGAGTAT TTCTGTTACG ATCTGTCCCC GAACCCCATC 
CAGAGCAGTA GCGATGAGAT TACGCTCTCC TTCAAGACCC TGCAGCGCAA TGGACTGATG 
CTTCACACCG GAAAGTCTGC AGACTACGTC AACCTGGCGC TGAAGAACGG AGCCGTCTCG 
CTGGTCATCA ACCTGGGCTC TGGGGCCTTC GAGGCCCTGG TGGAGCCCGT CAATGGGAAA 
TTTAATGACA ATGCCTGGCA TGATGTGAAG GTTACCAGGA ACCTTCGTCA GCACTCAGGC 
ATTGGACACG CTATGGTTAC AATATCCGTG GATGGGATTC TGACCACCAC TGGATATACG 
CAAGAGGACT ACACCATGCT AGGCTCGGAT GACTTTTTCT ATGTCGGCGG TAGTCCCAGC 
ACTGCCGATC TTCCTGGATC ACCTGTCAGC AACAACTTTA TGGGCTGCTT GAAAGAGGTT 
GTTTACAAAA ACAACGATGT AAGACTTGAG CTGTCTCGAC TGGCCAAGCT GGGCGACCCA 
AAGATGAAGG TGAGCGGTGT GGTGGCCTTC AAATGTGAGA ACGTGGCTAC TCTCGACCCT 
GTGACTTTTG AGACGCCTGA GTCATTCATT ACCCTTGACA AGTGGTCCGC CAAAAAAGCC 
GGCTCCATCT CTTTCGACTT CCGCACCACT GAACCCAACG GCCTGCTCCT CTTCAGCCAT 
GGAAAACCCA AACCCCAGCA ACAGAAAGAC CCTAAGAGCC CTAAAACATT GAAGGTGGAT 
TTCTTTGCCA TTGAGATGCT GGACGGCCAC CTCTACCTTC TTCTCGACAT GGGCTCAGGC 
ACCACCAAAA CTAGAGCTGT CAACAAGAAA GTCAATGACG GAGAATGGTA CCACGTGGAC 
TTCCAGAGAG ATGGAAGATC AGGCACCATT TCCGTCAACT CGATTCGCAC TCCCTACAAC 
GCTCCTGGTG AGAGTGAGAT ACTGGACCTG GACGATAAAC TGTACCTGGG CGGCCTGCCA 
GAGGACCGGG CCGGTCTGAT CTTCCCCACG GAGGTGTGGA CCGCTCTCCT AAATTACGGC 
TATGTGGGCT GTGTGCGGGA TCTGTTCATG GATGGCCAGA GCAAGGACAT TCGGCGGATA 
GCAGAGGCTC AGAGAGCGGT CGGGGTGAAG CCGTCTTGCT CTAAAGAACC CCCCAAACAG 
TGTCTGAGCA ACCCCTGTCT GAACAGCGGC ACCTGCAGAG AGGGCTGGAA CCGCTATGTG 
TGCGACTGCT CCGGGACGGG ATATCTGGGA CGCTCCTGTG AGAGAGATGC CACGATCCTC 
AGTTACGATG GTAGTAAGTT CATGAAGATC CAGCTGCCGG TGGTGATGCA CACAGAAGCG 
GAGGACGTGT CCCTGCGCTT TCGTTCTCAA CGGGCCTACG GTGTTCTAAT GGCGACCACA 
TCGCAAAACT CTGCTGATAC TCTGAGGCTG GAGCTGGACG GAGGGCGGGT CCGACTCACT 
GTCAATCTAG ACTGTATCAG GATAAACTGT ACTACCAGTA AAGGTCCAGA GACCATATTT 
TCGGGCCAGA ACTTGAATGA CAACGAATGG CACACAGTTC GTGTCGTCAG AAGAGGCAAA 
AGTCTAAAGC TGATGGTGGA CGACTTGCAG CCCTCTGAAG GTCAAATCAC TGGGGACCAC 
ACGCAGCTGG AGTTCCACAA TGTGGAAACG GGCATCGTTA CCGAGAAGCG CTACATGCCC 
GCTGTGCCCT CCAACTTCAT CGGACACCTG CAGGGTTTGT CCTTCAATGG CATGTCCTAC 
ATTGACTTGT GCAAGAACGG CGATATCGAC TACTGCGAAC TGAACGCCAT GATCGGCTAC 
AGGAGCATCG TGGCAGACCC AGTGACCTTC AAGTCTCGCT CCAGCTATGT GACTTTGCCC 
ACCCTGCAGG CCTACTATTC GATGCACCTC TTCTTCCAGT TTAAGACTAC TTCTCCAGAT 
GGGCTCATCC TGTACAACCG AGGAGATGGC AATGACTTTA TAGTGGTAGA GCTGGTTAAA 
GGATATCTCC ATTACGTGTC AGATCTGGGA AATGGAGCTC ATCTCATCAA AGGCAATTCC 
AACACACCGC TAAATGACAA TCACTGGCAC AACGTGATGA TCTCGCGAGA TACAAACAAC 
CTCCATACTG TCAAAATAGA CACCAAAATC ACCACACAGA CTACCATGGG AGCCAAGAAC 
CTGGATCTGA AAGGTGATCT GTATGTTGGA GGTGTGGCTA AAGATATGTA TAAGGACTTG 
CCAAAGCTGG TGCATTCAAA AGAGGGCTTC CAGGGCTGCC TGGCATCTGT GGACCTGAAC 
GGCCGCTTGC CAGACCTGCA GTCAGACGCA CTGTCCACCG CCGGCCAGGT GGAGAGAGGC 
TGCGAAGGTG AGGAAAACGT TGCATTGCTG AAAGCTGACT TGCAAGGCCC CAGCACTACC 
TGTCAGGAGG ACTCCTGCTC CAATCAGGGC GTGTGTCTGC AGCAGTGGGA GGGGTTTAGT 
TGCGACTGCA GCATGACATC ATATGGAGGG CCACTCTGCA ATGACCCGGG AACCACATAC 
ATATTCGGAC GTGATGGAGG GCTTATTGTG TACACATGGC CACCCAACGA CAGACCGAGC 
ACACGAGCTG ACCGACTGGC TGTCGGTTTT AGTACCCAGC AGAAGGATGC AGTGCTGGTT 
AGGGTGGACA GCTCTTCTGG CCTGGGAGAC TACCTTCAGC TTCAGATTGA AAGAGGCAAT 
ATCAAAGTGG TGTTCAATGT GGGCACCGAT GACATAAACA TTGAAGAAAC TTCAAAGTTT 
GTAAATGATG GAAAGTACCA CATAGTTCGC TTTACGAGAA GTGGTGGTAA CGCCACCTTG 
CAGGTGGATG ATCTGCCAGT CATTGAGCGC TACCCATCAG GCAACATTGA TAACGAACGC 
CTGGCGATTG CTAGACAGCG AATCCCATAT CGGCTTGGTC GAGTAGTTGA CGATTGGCTA 
CTCGACAAAG GTCGGCAGCT CACCATCTTC AACAGTCAGA CCACCATAAA GATTGGAGGC 
TGGGAGAAGG GCAGCCGGCC CTTCCAGGGT CAGCTCTCAG GGCTTTACTA CAATGGGCTA 
AAAGTACTCA ACATGGCCGC TGAGGGTGAC CCTAATGTGC GTGTGGAGGG TAGTGCCCGG 
CTGGTAGGAG ACATGCCCTC CTCTTCTATC ACGCCACAGT CCAGCGTCTC TGCTGCGGGA 
AATCGTTCAG AAACGTCACC CTCCATCACT GACATCACCA CTACAACGGC GTCCAACCGC 
CAGGGCAAGC AGACCACAAC TCCACAGGAC GATCTTCTCG TAGCGTCTGC AGAATGCCCC 
AGCGATGATG AGGACATTGA CCCTTGCGAC CCAAGTTCAG GTGGGTTAGC TCACCCACCA 
CTTCCCGAGG CCAAGGGTTA CCCGAGCCCA GAGGTGATTC GGGAGTCCAG CAGCACCACG 
GGCATGGTCG TAGGCATCGT TGCAGCCGCT GCACTCTGCA TCCTAATCCT GCTCTACGCC 
ATGTACAAAT ACAGAAACCG CGACGAAGGG TCTTACCACG TGGACGAGAG TCGTAACTAC 
ATTAGTAATT CTGCCACGCA GCCCAATGGA GCTGCCGTCA AAGAAAAGCC CATCGGCGTG 
CCCAAAAATA AAAAAGATAA AAAGAACAAG GACAAGGAGT ACTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304917.1
CDS	983..5473
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306642.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306642.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGGCCTAT 
GTAGGAGGTC TTGCGCACCT GATGATGGGC GACCAAGGGA AAAGAAAAGA GAGAGAAGAA 
TATATGGCCA CATTCAAGGG TTCGGAGTAT TTCTGTTACG ATCTGTCCCC GAACCCCATC 
CAGAGCAGTA GCGATGAGAT TACGCTCTCC TTCAAGACCC TGCAGCGCAA TGGACTGATG 
CTTCACACCG GAAAGTCTGC AGACTACGTC AACCTGGCGC TGAAGAACGG AGCCGTCTCG 
CTGGTCATCA ACCTGGGCTC TGGGGCCTTC GAGGCCCTGG TGGAGCCCGT CAATGGGAAA 
TTTAATGACA ATGCCTGGCA TGATGTGAAG GTTACCAGGA ACCTTCGTCA GCACTCAGGC 
ATTGGACACG CTATGGTTAC AATATCCGTG GATGGGATTC TGACCACCAC TGGATATACG 
CAAGAGGACT ACACCATGCT AGGCTCGGAT GACTTTTTCT ATGTCGGCGG TAGTCCCAGC 
ACTGCCGATC TTCCTGGATC ACCTGTCAGC AACAACTTTA TGGGCTGCTT GAAAGAGGTT 
GTTTACAAAA ACAACGATGT AAGACTTGAG CTGTCTCGAC TGGCCAAGCT GGGCGACCCA 
AAGATGAAGG TGAGCGGTGT GGTGGCCTTC AAATGTGAGA ACGTGGCTAC TCTCGACCCT 
GTGACTTTTG AGACGCCTGA GTCATTCATT ACCCTTGACA AGTGGTCCGC CAAAAAAGCC 
GGCTCCATCT CTTTCGACTT CCGCACCACT GAACCCAACG GCCTGCTCCT CTTCAGCCAT 
GGAAAACCCA AACCCCAGCA ACAGAAAGAC CCTAAGAGCC CTAAAACATT GAAGGTGGAT 
TTCTTTGCCA TTGAGATGCT GGACGGCCAC CTCTACCTTC TTCTCGACAT GGGCTCAGGC 
ACCACCAAAA CTAGAGCTGT CAACAAGAAA GTCAATGACG GAGAATGGTA CCACGTGGAC 
TTCCAGAGAG ATGGAAGATC AGGCACCATT TCCGTCAACT CGATTCGCAC TCCCTACAAC 
GCTCCTGGTG AGAGTGAGAT ACTGGACCTG GACGATAAAC TGTACCTGGG CGGCCTGCCA 
GAGGACCGGG CCGGTCTGAT CTTCCCCACG GAGGTGTGGA CCGCTCTCCT AAATTACGGC 
TATGTGGGCT GTGTGCGGGA TCTGTTCATG GATGGCCAGA GCAAGGACAT TCGGCGGATA 
GCAGAGGCTC AGAGAGCGGT CGGGGTGAAG CCGTCTTGCT CTAAAGAACC CCCCAAACAG 
TGTCTGAGCA ACCCCTGTCT GAACAGCGGC ACCTGCAGAG AGGGCTGGAA CCGCTATGTG 
TGCGACTGCT CCGGGACGGG ATATCTGGGA CGCTCCTGTG AGAGAGATGC CACGATCCTC 
AGTTACGATG GTAGTAAGTT CATGAAGATC CAGCTGCCGG TGGTGATGCA CACAGAAGCG 
GAGGACGTGT CCCTGCGCTT TCGTTCTCAA CGGGCCTACG GTGTTCTAAT GGCGACCACA 
TCGCAAAACT CTGCTGATAC TCTGAGGCTG GAGCTGGACG GAGGGCGGGT CCGACTCACT 
GTCAATCTAG ACTGTATCAG GATAAACTGT ACTACCAGTA AAGGTCCAGA GACCATATTT 
TCGGGCCAGA ACTTGAATGA CAACGAATGG CACACAGTTC GTGTCGTCAG AAGAGGCAAA 
AGTCTAAAGC TGATGGTGGA CGACTTGCAG CCCTCTGAAG GTCAAATCAC TGGGGACCAC 
ACGCAGCTGG AGTTCCACAA TGTGGAAACG GGCATCGTTA CCGAGAAGCG CTACATGCCC 
GCTGTGCCCT CCAACTTCAT CGGACACCTG CAGGGTTTGT CCTTCAATGG CATGTCCTAC 
ATTGACTTGT GCAAGAACGG CGATATCGAC TACTGCGAAC TGAACGCCAT GATCGGCTAC 
AGGAGCATCG TGGCAGACCC AGTGACCTTC AAGTCTCGCT CCAGCTATGT GACTTTGCCC 
ACCCTGCAGG CCTACTATTC GATGCACCTC TTCTTCCAGT TTAAGACTAC TTCTCCAGAT 
GGGCTCATCC TGTACAACCG AGGAGATGGC AATGACTTTA TAGTGGTAGA GCTGGTTAAA 
GGATATCTCC ATTACGTGTC AGATCTGGGA AATGGAGCTC ATCTCATCAA AGGCAATTCC 
AACACACCGC TAAATGACAA TCACTGGCAC AACGTGATGA TCTCGCGAGA TACAAACAAC 
CTCCATACTG TCAAAATAGA CACCAAAATC ACCACACAGA CTACCATGGG AGCCAAGAAC 
CTGGATCTGA AAGGTGATCT GTATGTTGGA GGTGTGGCTA AAGATATGTA TAAGGACTTG 
CCAAAGCTGG TGCATTCAAA AGAGGGCTTC CAGGGCTGCC TGGCATCTGT GGACCTGAAC 
GGCCGCTTGC CAGACCTGCA GTCAGACGCA CTGTCCACCG CCGGCCAGGT GGAGAGAGGC 
TGCGAAGGTG AGGAAAACGT TGCATTGCTG AAAGCTGACT TGCAAGGCCC CAGCACTACC 
TGTCAGGAGG ACTCCTGCTC CAATCAGGGC GTGTGTCTGC AGCAGTGGGA GGGGTTTAGT 
TGCGACTGCA GCATGACATC ATATGGAGGG CCACTCTGCA ATGACCCGGG AACCACATAC 
ATATTCGGAC GTGATGGAGG GCTTATTGTG TACACATGGC CACCCAACGA CAGACCGAGC 
ACACGAGCTG ACCGACTGGC TGTCGGTTTT AGTACCCAGC AGAAGGATGC AGTGCTGGTT 
AGGGTGGACA GCTCTTCTGG CCTGGGAGAC TACCTTCAGC TTCAGATTGA AAGAGGCAAT 
ATCAAAGTGG TGTTCAATGT GGGCACCGAT GACATAAACA TTGAAGAAAC TTCAAAGTTT 
GTAAATGATG GAAAGTACCA CATAGTTCGC TTTACGAGAA GTGGTGGTAA CGCCACCTTG 
CAGGTGGATG ATCTGCCAGT CATTGAGCGC TACCCATCAG GCAACATTGA TAACGAACGC 
CTGGCGATTG CTAGACAGCG AATCCCATAT CGGCTTGGTC GAGTAGTTGA CGATTGGCTA 
CTCGACAAAG GTCGGCAGCT CACCATCTTC AACAGTCAGA CCACCATAAA GATTGGAGGC 
TGGGAGAAGG GCAGCCGGCC CTTCCAGGGT CAGCTCTCAG GGCTTTACTA CAATGGGCTA 
AAAGTACTCA ACATGGCCGC TGAGGGTGAC CCTAATGTGC GTGTGGAGGG TAGTGCCCGG 
CTGGTAGGAG ACATGCCCTC CTCTTCTATC ACGCCACAGT CCAGCGTCTC TGCTGCGGGA 
AATCGTTCAG AAACGTCACC CTCCATCACT GACATCACCA CTACAACGGC GTCCAACCGC 
CAGGGCAAGC AGACCACAAC TCCACAGGAC GATCTTCTCG TAGCGTCTGC AGAATGCCCC 
AGCGATGATG AGGACATTGA CCCTTGCGAC CCAAGTTCAG GTGGGTTAGC TCACCCACCA 
CTTCCCGAGG CCAAGGGTTA CCCGAGCCCA GAGGTGATTC GGGAGTCCAG CAGCACCACG 
GGCATGGTCG TAGGCATCGT TGCAGCCGCT GCACTCTGCA TCCTAATCCT GCTCTACGCC 
ATGTACAAAT ACAGAAACCG CGACGAAGGG TCTTACCACG TGGACGAGAG TCGTAACTAC 
ATTAGTAATT CTGCCACGCA GCCCAATGGA GCTGCCGTCA AAGAAAAGCC CATCGGCGTG 
CCCAAAAATA AAAAAGATAA AAAGAACAAG GACAAGGAGT ACTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306643.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304918.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa44654
Clone ID Related Accession (Same CDS sequence)	XM_009306643.3 , XM_009306643.2
Accession Version	XM_009306643.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306643.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304918.1
CDS	985..5469
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306643.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306643.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306643.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304918.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X3, mRNA.

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CloneID	ODa44654
Clone ID Related Accession (Same CDS sequence)	XM_009306643.3 , XM_009306643.2
Accession Version	XM_009306643.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304918.1
CDS	983..5467
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306643.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306643.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGCAACAT TGATAACGAA 
CGCCTGGCGA TTGCTAGACA GCGAATCCCA TATCGGCTTG GTCGAGTAGT TGACGATTGG 
CTACTCGACA AAGGTCGGCA GCTCACCATC TTCAACAGTC AGACCACCAT AAAGATTGGA 
GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG GGTCAGCTCT CAGGGCTTTA CTACAATGGG 
CTAAAAGTAC TCAACATGGC CGCTGAGGGT GACCCTAATG TGCGTGTGGA GGGTAGTGCC 
CGGCTGGTAG GAGACATGCC CTCCTCTTCT ATCACGCCAC AGTCCAGCGT CTCTGCTGCG 
GGAAATCGTT CAGAAACGTC ACCCTCCATC ACTGACATCA CCACTACAAC GGCGTCCAAC 
CGCCAGGGCA AGCAGACCAC AACTCCACAG GACGATCTTC TCGTAGCGTC TGCAGAATGC 
CCCAGCGATG ATGAGGACAT TGACCCTTGC GACCCAAGTT CAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306644.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304919.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X4, mRNA.

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CloneID	ODa44655
Clone ID Related Accession (Same CDS sequence)	XM_009306644.2 , XM_009306644.3
Accession Version	XM_009306644.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG TTGCATTGCT GAAAGCTGAC TTGCAAGGCC CCAGCACTAC CTGTCAGGAG 
GACTCCTGCT CCAATCAGGG CGTGTGTCTG CAGCAGTGGG AGGGGTTTAG TTGCGACTGC 
AGCATGACAT CATATGGAGG GCCACTCTGC AATGACCCGG GAACCACATA CATATTCGGA 
CGTGATGGAG GGCTTATTGT GTACACATGG CCACCCAACG ACAGACCGAG CACACGAGCT 
GACCGACTGG CTGTCGGTTT TAGTACCCAG CAGAAGGATG CAGTGCTGGT TAGGGTGGAC 
AGCTCTTCTG GCCTGGGAGA CTACCTTCAG CTTCAGATTG AAAGAGGCAA TATCAAAGTG 
GTGTTCAATG TGGGCACCGA TGACATAAAC ATTGAAGAAA CTTCAAAGTT TGTAAATGAT 
GGAAAGTACC ACATAGTTCG CTTTACGAGA AGTGGTGGTA ACGCCACCTT GCAGGTGGAT 
GATCTGCCAG TCATTGAGCG CTACCCATCA GGCAACATTG ATAACGAACG CCTGGCGATT 
GCTAGACAGC GAATCCCATA TCGGCTTGGT CGAGTAGTTG ACGATTGGCT ACTCGACAAA 
GGTCGGCAGC TCACCATCTT CAACAGTCAG ACCACCATAA AGATTGGAGG CTGGGAGAAG 
GGCAGCCGGC CCTTCCAGGG TCAGCTCTCA GGGCTTTACT ACAATGGGCT AAAAGTACTC 
AACATGGCCG CTGAGGGTGA CCCTAATGTG CGTGTGGAGG GTAGTGCCCG GCTGGTAGGA 
GACATGCCCT CCTCTTCTAT CACGCCACAG TCCAGCGTCT CTGCTGCGGG AAATCGTTCA 
GAAACGTCAC CCTCCATCAC TGACATCACC ACTACAACGG CGTCCAACCG CCAGGGCAAG 
CAGACCACAA CTCCACAGGA CGATCTTCTC GTAGCGTCTG CAGAATGCCC CAGCGATGAT 
GAGGACATTG ACCCTTGCGA CCCAAGTTCA GGTGGGTTAG CTCACCCACC ACTTCCCGAG 
GCCAAGGGTT ACCCGAGCCC AGAGGTGATT CGGGAGTCCA GCAGCACCAC GGGCATGGTC 
GTAGGCATCG TTGCAGCCGC TGCACTCTGC ATCCTAATCC TGCTCTACGC CATGTACAAA 
TACAGAAACC GCGACGAAGG GTCTTACCAC GTGGACGAGA GTCGTAACTA CATTAGTAAT 
TCTGCCACGC AGCCCAATGG AGCTGCCGTC AAAGAAAAGC CCATCGGCGT GCCCAAAAAT 
AAAAAAGATA AAAAGAACAA GGACAAGGAG TACTACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304919.1
CDS	983..5464
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306644.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306644.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG TTGCATTGCT GAAAGCTGAC TTGCAAGGCC CCAGCACTAC CTGTCAGGAG 
GACTCCTGCT CCAATCAGGG CGTGTGTCTG CAGCAGTGGG AGGGGTTTAG TTGCGACTGC 
AGCATGACAT CATATGGAGG GCCACTCTGC AATGACCCGG GAACCACATA CATATTCGGA 
CGTGATGGAG GGCTTATTGT GTACACATGG CCACCCAACG ACAGACCGAG CACACGAGCT 
GACCGACTGG CTGTCGGTTT TAGTACCCAG CAGAAGGATG CAGTGCTGGT TAGGGTGGAC 
AGCTCTTCTG GCCTGGGAGA CTACCTTCAG CTTCAGATTG AAAGAGGCAA TATCAAAGTG 
GTGTTCAATG TGGGCACCGA TGACATAAAC ATTGAAGAAA CTTCAAAGTT TGTAAATGAT 
GGAAAGTACC ACATAGTTCG CTTTACGAGA AGTGGTGGTA ACGCCACCTT GCAGGTGGAT 
GATCTGCCAG TCATTGAGCG CTACCCATCA GGCAACATTG ATAACGAACG CCTGGCGATT 
GCTAGACAGC GAATCCCATA TCGGCTTGGT CGAGTAGTTG ACGATTGGCT ACTCGACAAA 
GGTCGGCAGC TCACCATCTT CAACAGTCAG ACCACCATAA AGATTGGAGG CTGGGAGAAG 
GGCAGCCGGC CCTTCCAGGG TCAGCTCTCA GGGCTTTACT ACAATGGGCT AAAAGTACTC 
AACATGGCCG CTGAGGGTGA CCCTAATGTG CGTGTGGAGG GTAGTGCCCG GCTGGTAGGA 
GACATGCCCT CCTCTTCTAT CACGCCACAG TCCAGCGTCT CTGCTGCGGG AAATCGTTCA 
GAAACGTCAC CCTCCATCAC TGACATCACC ACTACAACGG CGTCCAACCG CCAGGGCAAG 
CAGACCACAA CTCCACAGGA CGATCTTCTC GTAGCGTCTG CAGAATGCCC CAGCGATGAT 
GAGGACATTG ACCCTTGCGA CCCAAGTTCA GGTGGGTTAG CTCACCCACC ACTTCCCGAG 
GCCAAGGGTT ACCCGAGCCC AGAGGTGATT CGGGAGTCCA GCAGCACCAC GGGCATGGTC 
GTAGGCATCG TTGCAGCCGC TGCACTCTGC ATCCTAATCC TGCTCTACGC CATGTACAAA 
TACAGAAACC GCGACGAAGG GTCTTACCAC GTGGACGAGA GTCGTAACTA CATTAGTAAT 
TCTGCCACGC AGCCCAATGG AGCTGCCGTC AAAGAAAAGC CCATCGGCGT GCCCAAAAAT 
AAAAAAGATA AAAAGAACAA GGACAAGGAG TACTACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306644.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304919.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X4, mRNA.

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CloneID	ODa44655
Clone ID Related Accession (Same CDS sequence)	XM_009306644.2 , XM_009306644.3
Accession Version	XM_009306644.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306644.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG TTGCATTGCT GAAAGCTGAC TTGCAAGGCC CCAGCACTAC CTGTCAGGAG 
GACTCCTGCT CCAATCAGGG CGTGTGTCTG CAGCAGTGGG AGGGGTTTAG TTGCGACTGC 
AGCATGACAT CATATGGAGG GCCACTCTGC AATGACCCGG GAACCACATA CATATTCGGA 
CGTGATGGAG GGCTTATTGT GTACACATGG CCACCCAACG ACAGACCGAG CACACGAGCT 
GACCGACTGG CTGTCGGTTT TAGTACCCAG CAGAAGGATG CAGTGCTGGT TAGGGTGGAC 
AGCTCTTCTG GCCTGGGAGA CTACCTTCAG CTTCAGATTG AAAGAGGCAA TATCAAAGTG 
GTGTTCAATG TGGGCACCGA TGACATAAAC ATTGAAGAAA CTTCAAAGTT TGTAAATGAT 
GGAAAGTACC ACATAGTTCG CTTTACGAGA AGTGGTGGTA ACGCCACCTT GCAGGTGGAT 
GATCTGCCAG TCATTGAGCG CTACCCATCA GGCAACATTG ATAACGAACG CCTGGCGATT 
GCTAGACAGC GAATCCCATA TCGGCTTGGT CGAGTAGTTG ACGATTGGCT ACTCGACAAA 
GGTCGGCAGC TCACCATCTT CAACAGTCAG ACCACCATAA AGATTGGAGG CTGGGAGAAG 
GGCAGCCGGC CCTTCCAGGG TCAGCTCTCA GGGCTTTACT ACAATGGGCT AAAAGTACTC 
AACATGGCCG CTGAGGGTGA CCCTAATGTG CGTGTGGAGG GTAGTGCCCG GCTGGTAGGA 
GACATGCCCT CCTCTTCTAT CACGCCACAG TCCAGCGTCT CTGCTGCGGG AAATCGTTCA 
GAAACGTCAC CCTCCATCAC TGACATCACC ACTACAACGG CGTCCAACCG CCAGGGCAAG 
CAGACCACAA CTCCACAGGA CGATCTTCTC GTAGCGTCTG CAGAATGCCC CAGCGATGAT 
GAGGACATTG ACCCTTGCGA CCCAAGTTCA GGTGGGTTAG CTCACCCACC ACTTCCCGAG 
GCCAAGGGTT ACCCGAGCCC AGAGGTGATT CGGGAGTCCA GCAGCACCAC GGGCATGGTC 
GTAGGCATCG TTGCAGCCGC TGCACTCTGC ATCCTAATCC TGCTCTACGC CATGTACAAA 
TACAGAAACC GCGACGAAGG GTCTTACCAC GTGGACGAGA GTCGTAACTA CATTAGTAAT 
TCTGCCACGC AGCCCAATGG AGCTGCCGTC AAAGAAAAGC CCATCGGCGT GCCCAAAAAT 
AAAAAAGATA AAAAGAACAA GGACAAGGAG TACTACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304919.1
CDS	986..5467
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306644.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306644.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG TTGCATTGCT GAAAGCTGAC TTGCAAGGCC CCAGCACTAC CTGTCAGGAG 
GACTCCTGCT CCAATCAGGG CGTGTGTCTG CAGCAGTGGG AGGGGTTTAG TTGCGACTGC 
AGCATGACAT CATATGGAGG GCCACTCTGC AATGACCCGG GAACCACATA CATATTCGGA 
CGTGATGGAG GGCTTATTGT GTACACATGG CCACCCAACG ACAGACCGAG CACACGAGCT 
GACCGACTGG CTGTCGGTTT TAGTACCCAG CAGAAGGATG CAGTGCTGGT TAGGGTGGAC 
AGCTCTTCTG GCCTGGGAGA CTACCTTCAG CTTCAGATTG AAAGAGGCAA TATCAAAGTG 
GTGTTCAATG TGGGCACCGA TGACATAAAC ATTGAAGAAA CTTCAAAGTT TGTAAATGAT 
GGAAAGTACC ACATAGTTCG CTTTACGAGA AGTGGTGGTA ACGCCACCTT GCAGGTGGAT 
GATCTGCCAG TCATTGAGCG CTACCCATCA GGCAACATTG ATAACGAACG CCTGGCGATT 
GCTAGACAGC GAATCCCATA TCGGCTTGGT CGAGTAGTTG ACGATTGGCT ACTCGACAAA 
GGTCGGCAGC TCACCATCTT CAACAGTCAG ACCACCATAA AGATTGGAGG CTGGGAGAAG 
GGCAGCCGGC CCTTCCAGGG TCAGCTCTCA GGGCTTTACT ACAATGGGCT AAAAGTACTC 
AACATGGCCG CTGAGGGTGA CCCTAATGTG CGTGTGGAGG GTAGTGCCCG GCTGGTAGGA 
GACATGCCCT CCTCTTCTAT CACGCCACAG TCCAGCGTCT CTGCTGCGGG AAATCGTTCA 
GAAACGTCAC CCTCCATCAC TGACATCACC ACTACAACGG CGTCCAACCG CCAGGGCAAG 
CAGACCACAA CTCCACAGGA CGATCTTCTC GTAGCGTCTG CAGAATGCCC CAGCGATGAT 
GAGGACATTG ACCCTTGCGA CCCAAGTTCA GGTGGGTTAG CTCACCCACC ACTTCCCGAG 
GCCAAGGGTT ACCCGAGCCC AGAGGTGATT CGGGAGTCCA GCAGCACCAC GGGCATGGTC 
GTAGGCATCG TTGCAGCCGC TGCACTCTGC ATCCTAATCC TGCTCTACGC CATGTACAAA 
TACAGAAACC GCGACGAAGG GTCTTACCAC GTGGACGAGA GTCGTAACTA CATTAGTAAT 
TCTGCCACGC AGCCCAATGG AGCTGCCGTC AAAGAAAAGC CCATCGGCGT GCCCAAAAAT 
AAAAAAGATA AAAAGAACAA GGACAAGGAG TACTACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306645.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304920.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa44656
Clone ID Related Accession (Same CDS sequence)	XM_009306645.2 , XM_009306645.3
Accession Version	XM_009306645.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG AGAGAGAAGA ATATATGGCC 
ACATTCAAGG GTTCGGAGTA TTTCTGTTAC GATCTGTCCC CGAACCCCAT CCAGAGCAGT 
AGCGATGAGA TTACGCTCTC CTTCAAGACC CTGCAGCGCA ATGGACTGAT GCTTCACACC 
GGAAAGTCTG CAGACTACGT CAACCTGGCG CTGAAGAACG GAGCCGTCTC GCTGGTCATC 
AACCTGGGCT CTGGGGCCTT CGAGGCCCTG GTGGAGCCCG TCAATGGGAA ATTTAATGAC 
AATGCCTGGC ATGATGTGAA GGTTACCAGG AACCTTCGTC AGCACTCAGG CATTGGACAC 
GCTATGGTTA CAATATCCGT GGATGGGATT CTGACCACCA CTGGATATAC GCAAGAGGAC 
TACACCATGC TAGGCTCGGA TGACTTTTTC TATGTCGGCG GTAGTCCCAG CACTGCCGAT 
CTTCCTGGAT CACCTGTCAG CAACAACTTT ATGGGCTGCT TGAAAGAGGT TGTTTACAAA 
AACAACGATG TAAGACTTGA GCTGTCTCGA CTGGCCAAGC TGGGCGACCC AAAGATGAAG 
GTGAGCGGTG TGGTGGCCTT CAAATGTGAG AACGTGGCTA CTCTCGACCC TGTGACTTTT 
GAGACGCCTG AGTCATTCAT TACCCTTGAC AAGTGGTCCG CCAAAAAAGC CGGCTCCATC 
TCTTTCGACT TCCGCACCAC TGAACCCAAC GGCCTGCTCC TCTTCAGCCA TGGAAAACCC 
AAACCCCAGC AACAGAAAGA CCCTAAGAGC CCTAAAACAT TGAAGGTGGA TTTCTTTGCC 
ATTGAGATGC TGGACGGCCA CCTCTACCTT CTTCTCGACA TGGGCTCAGG CACCACCAAA 
ACTAGAGCTG TCAACAAGAA AGTCAATGAC GGAGAATGGT ACCACGTGGA CTTCCAGAGA 
GATGGAAGAT CAGGCACCAT TTCCGTCAAC TCGATTCGCA CTCCCTACAA CGCTCCTGGT 
GAGAGTGAGA TACTGGACCT GGACGATAAA CTGTACCTGG GCGGCCTGCC AGAGGACCGG 
GCCGGTCTGA TCTTCCCCAC GGAGGTGTGG ACCGCTCTCC TAAATTACGG CTATGTGGGC 
TGTGTGCGGG ATCTGTTCAT GGATGGCCAG AGCAAGGACA TTCGGCGGAT AGCAGAGGCT 
CAGAGAGCGG TCGGGGTGAA GCCGTCTTGC TCTAAAGAAC CCCCCAAACA GTGTCTGAGC 
AACCCCTGTC TGAACAGCGG CACCTGCAGA GAGGGCTGGA ACCGCTATGT GTGCGACTGC 
TCCGGGACGG GATATCTGGG ACGCTCCTGT GAGAGAGATG CCACGATCCT CAGTTACGAT 
GGTAGTAAGT TCATGAAGAT CCAGCTGCCG GTGGTGATGC ACACAGAAGC GGAGGACGTG 
TCCCTGCGCT TTCGTTCTCA ACGGGCCTAC GGTGTTCTAA TGGCGACCAC ATCGCAAAAC 
TCTGCTGATA CTCTGAGGCT GGAGCTGGAC GGAGGGCGGG TCCGACTCAC TGTCAATCTA 
GACTGTATCA GGATAAACTG TACTACCAGT AAAGGTCCAG AGACCATATT TTCGGGCCAG 
AACTTGAATG ACAACGAATG GCACACAGTT CGTGTCGTCA GAAGAGGCAA AAGTCTAAAG 
CTGATGGTGG ACGACTTGCA GCCCTCTGAA GGTCAAATCA CTGGGGACCA CACGCAGCTG 
GAGTTCCACA ATGTGGAAAC GGGCATCGTT ACCGAGAAGC GCTACATGCC CGCTGTGCCC 
TCCAACTTCA TCGGACACCT GCAGGGTTTG TCCTTCAATG GCATGTCCTA CATTGACTTG 
TGCAAGAACG GCGATATCGA CTACTGCGAA CTGAACGCCA TGATCGGCTA CAGGAGCATC 
GTGGCAGACC CAGTGACCTT CAAGTCTCGC TCCAGCTATG TGACTTTGCC CACCCTGCAG 
GCCTACTATT CGATGCACCT CTTCTTCCAG TTTAAGACTA CTTCTCCAGA TGGGCTCATC 
CTGTACAACC GAGGAGATGG CAATGACTTT ATAGTGGTAG AGCTGGTTAA AGGATATCTC 
CATTACGTGT CAGATCTGGG AAATGGAGCT CATCTCATCA AAGGCAATTC CAACACACCG 
CTAAATGACA ATCACTGGCA CAACGTGATG ATCTCGCGAG ATACAAACAA CCTCCATACT 
GTCAAAATAG ACACCAAAAT CACCACACAG ACTACCATGG GAGCCAAGAA CCTGGATCTG 
AAAGGTGATC TGTATGTTGG AGGTGTGGCT AAAGATATGT ATAAGGACTT GCCAAAGCTG 
GTGCATTCAA AAGAGGGCTT CCAGGGCTGC CTGGCATCTG TGGACCTGAA CGGCCGCTTG 
CCAGACCTGC AGTCAGACGC ACTGTCCACC GCCGGCCAGG TGGAGAGAGG CTGCGAAGGT 
GAGGAAAACG TTGCATTGCT GAAAGCTGAC TTGCAAGGCC CCAGCACTAC CTGTCAGGAG 
GACTCCTGCT CCAATCAGGG CGTGTGTCTG CAGCAGTGGG AGGGGTTTAG TTGCGACTGC 
AGCATGACAT CATATGGAGG GCCACTCTGC AATGACCCGG GAACCACATA CATATTCGGA 
CGTGATGGAG GGCTTATTGT GTACACATGG CCACCCAACG ACAGACCGAG CACACGAGCT 
GACCGACTGG CTGTCGGTTT TAGTACCCAG CAGAAGGATG CAGTGCTGGT TAGGGTGGAC 
AGCTCTTCTG GCCTGGGAGA CTACCTTCAG CTTCAGATTG AAAGAGGCAA TATCAAAGTG 
GTGTTCAATG TGGGCACCGA TGACATAAAC ATTGAAGAAA CTTCAAAGTT TGTAAATGAT 
GGAAAGTACC ACATAGTTCG CTTTACGAGA AGTGGTGGTA ACGCCACCTT GCAGGTGGAT 
GATCTGCCAG TCATTGAGCG CTACCCATCA GGCAACATTG ATAACGAACG CCTGGCGATT 
GCTAGACAGC GAATCCCATA TCGGCTTGGT CGAGTAGTTG ACGATTGGCT ACTCGACAAA 
GGTCGGCAGC TCACCATCTT CAACAGTCAG ACCACCATAA AGATTGGAGG CTGGGAGAAG 
GGCAGCCGGC CCTTCCAGGG TCAGCTCTCA GGGCTTTACT ACAATGGGCT AAAAGTACTC 
AACATGGCCG CTGAGGGTGA CCCTAATGTG CGTGTGGAGG GTAGTGCCCG GCTGGTAGGA 
GACATGCCCT CCTCTTCTAT CACGCCACAG TCCAGCGTCT CTGCTGCGGG AAATCGTTCA 
GAAACGTCAC CCTCCATCAC TGACATCACC ACTACAACGG CGTCCAACCG CCAGGGCAAG 
CAGACCACAA CTCCACAGGA CGATCTTCTC GTAGCGTCTG CAGAATGCCC CAGCGATGAT 
GAGGACATTG ACCCTTGCGA CCCAAGTTCA GGTGGGTTAG CTCACCCACC ACTTCCCGAG 
GCCAAGGGTT ACCCGAGCCC AGAGGTGATT CGGGAGTCCA GCAGCACCAC GGGCATGGTC 
GTAGGCATCG TTGCAGCCGC TGCACTCTGC ATCCTAATCC TGCTCTACGC CATGTACAAA 
TACAGAAACC GCGACGAAGG GTCTTACCAC GTGGACGAGA GTCGTAACTA CATTAGTAAT 
TCTGCCACGC AGCCCAATGG AGCTGCCGTC AAAGAAAAGC CCATCGGCGT GCCCAAAAAT 
AAAAAAGATA AAAAGAACAA GGACAAGGAG TACTACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304920.1
CDS	983..5464
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306645.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306645.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG AGAGAGAAGA ATATATGGCC 
ACATTCAAGG GTTCGGAGTA TTTCTGTTAC GATCTGTCCC CGAACCCCAT CCAGAGCAGT 
AGCGATGAGA TTACGCTCTC CTTCAAGACC CTGCAGCGCA ATGGACTGAT GCTTCACACC 
GGAAAGTCTG CAGACTACGT CAACCTGGCG CTGAAGAACG GAGCCGTCTC GCTGGTCATC 
AACCTGGGCT CTGGGGCCTT CGAGGCCCTG GTGGAGCCCG TCAATGGGAA ATTTAATGAC 
AATGCCTGGC ATGATGTGAA GGTTACCAGG AACCTTCGTC AGCACTCAGG CATTGGACAC 
GCTATGGTTA CAATATCCGT GGATGGGATT CTGACCACCA CTGGATATAC GCAAGAGGAC 
TACACCATGC TAGGCTCGGA TGACTTTTTC TATGTCGGCG GTAGTCCCAG CACTGCCGAT 
CTTCCTGGAT CACCTGTCAG CAACAACTTT ATGGGCTGCT TGAAAGAGGT TGTTTACAAA 
AACAACGATG TAAGACTTGA GCTGTCTCGA CTGGCCAAGC TGGGCGACCC AAAGATGAAG 
GTGAGCGGTG TGGTGGCCTT CAAATGTGAG AACGTGGCTA CTCTCGACCC TGTGACTTTT 
GAGACGCCTG AGTCATTCAT TACCCTTGAC AAGTGGTCCG CCAAAAAAGC CGGCTCCATC 
TCTTTCGACT TCCGCACCAC TGAACCCAAC GGCCTGCTCC TCTTCAGCCA TGGAAAACCC 
AAACCCCAGC AACAGAAAGA CCCTAAGAGC CCTAAAACAT TGAAGGTGGA TTTCTTTGCC 
ATTGAGATGC TGGACGGCCA CCTCTACCTT CTTCTCGACA TGGGCTCAGG CACCACCAAA 
ACTAGAGCTG TCAACAAGAA AGTCAATGAC GGAGAATGGT ACCACGTGGA CTTCCAGAGA 
GATGGAAGAT CAGGCACCAT TTCCGTCAAC TCGATTCGCA CTCCCTACAA CGCTCCTGGT 
GAGAGTGAGA TACTGGACCT GGACGATAAA CTGTACCTGG GCGGCCTGCC AGAGGACCGG 
GCCGGTCTGA TCTTCCCCAC GGAGGTGTGG ACCGCTCTCC TAAATTACGG CTATGTGGGC 
TGTGTGCGGG ATCTGTTCAT GGATGGCCAG AGCAAGGACA TTCGGCGGAT AGCAGAGGCT 
CAGAGAGCGG TCGGGGTGAA GCCGTCTTGC TCTAAAGAAC CCCCCAAACA GTGTCTGAGC 
AACCCCTGTC TGAACAGCGG CACCTGCAGA GAGGGCTGGA ACCGCTATGT GTGCGACTGC 
TCCGGGACGG GATATCTGGG ACGCTCCTGT GAGAGAGATG CCACGATCCT CAGTTACGAT 
GGTAGTAAGT TCATGAAGAT CCAGCTGCCG GTGGTGATGC ACACAGAAGC GGAGGACGTG 
TCCCTGCGCT TTCGTTCTCA ACGGGCCTAC GGTGTTCTAA TGGCGACCAC ATCGCAAAAC 
TCTGCTGATA CTCTGAGGCT GGAGCTGGAC GGAGGGCGGG TCCGACTCAC TGTCAATCTA 
GACTGTATCA GGATAAACTG TACTACCAGT AAAGGTCCAG AGACCATATT TTCGGGCCAG 
AACTTGAATG ACAACGAATG GCACACAGTT CGTGTCGTCA GAAGAGGCAA AAGTCTAAAG 
CTGATGGTGG ACGACTTGCA GCCCTCTGAA GGTCAAATCA CTGGGGACCA CACGCAGCTG 
GAGTTCCACA ATGTGGAAAC GGGCATCGTT ACCGAGAAGC GCTACATGCC CGCTGTGCCC 
TCCAACTTCA TCGGACACCT GCAGGGTTTG TCCTTCAATG GCATGTCCTA CATTGACTTG 
TGCAAGAACG GCGATATCGA CTACTGCGAA CTGAACGCCA TGATCGGCTA CAGGAGCATC 
GTGGCAGACC CAGTGACCTT CAAGTCTCGC TCCAGCTATG TGACTTTGCC CACCCTGCAG 
GCCTACTATT CGATGCACCT CTTCTTCCAG TTTAAGACTA CTTCTCCAGA TGGGCTCATC 
CTGTACAACC GAGGAGATGG CAATGACTTT ATAGTGGTAG AGCTGGTTAA AGGATATCTC 
CATTACGTGT CAGATCTGGG AAATGGAGCT CATCTCATCA AAGGCAATTC CAACACACCG 
CTAAATGACA ATCACTGGCA CAACGTGATG ATCTCGCGAG ATACAAACAA CCTCCATACT 
GTCAAAATAG ACACCAAAAT CACCACACAG ACTACCATGG GAGCCAAGAA CCTGGATCTG 
AAAGGTGATC TGTATGTTGG AGGTGTGGCT AAAGATATGT ATAAGGACTT GCCAAAGCTG 
GTGCATTCAA AAGAGGGCTT CCAGGGCTGC CTGGCATCTG TGGACCTGAA CGGCCGCTTG 
CCAGACCTGC AGTCAGACGC ACTGTCCACC GCCGGCCAGG TGGAGAGAGG CTGCGAAGGT 
GAGGAAAACG TTGCATTGCT GAAAGCTGAC TTGCAAGGCC CCAGCACTAC CTGTCAGGAG 
GACTCCTGCT CCAATCAGGG CGTGTGTCTG CAGCAGTGGG AGGGGTTTAG TTGCGACTGC 
AGCATGACAT CATATGGAGG GCCACTCTGC AATGACCCGG GAACCACATA CATATTCGGA 
CGTGATGGAG GGCTTATTGT GTACACATGG CCACCCAACG ACAGACCGAG CACACGAGCT 
GACCGACTGG CTGTCGGTTT TAGTACCCAG CAGAAGGATG CAGTGCTGGT TAGGGTGGAC 
AGCTCTTCTG GCCTGGGAGA CTACCTTCAG CTTCAGATTG AAAGAGGCAA TATCAAAGTG 
GTGTTCAATG TGGGCACCGA TGACATAAAC ATTGAAGAAA CTTCAAAGTT TGTAAATGAT 
GGAAAGTACC ACATAGTTCG CTTTACGAGA AGTGGTGGTA ACGCCACCTT GCAGGTGGAT 
GATCTGCCAG TCATTGAGCG CTACCCATCA GGCAACATTG ATAACGAACG CCTGGCGATT 
GCTAGACAGC GAATCCCATA TCGGCTTGGT CGAGTAGTTG ACGATTGGCT ACTCGACAAA 
GGTCGGCAGC TCACCATCTT CAACAGTCAG ACCACCATAA AGATTGGAGG CTGGGAGAAG 
GGCAGCCGGC CCTTCCAGGG TCAGCTCTCA GGGCTTTACT ACAATGGGCT AAAAGTACTC 
AACATGGCCG CTGAGGGTGA CCCTAATGTG CGTGTGGAGG GTAGTGCCCG GCTGGTAGGA 
GACATGCCCT CCTCTTCTAT CACGCCACAG TCCAGCGTCT CTGCTGCGGG AAATCGTTCA 
GAAACGTCAC CCTCCATCAC TGACATCACC ACTACAACGG CGTCCAACCG CCAGGGCAAG 
CAGACCACAA CTCCACAGGA CGATCTTCTC GTAGCGTCTG CAGAATGCCC CAGCGATGAT 
GAGGACATTG ACCCTTGCGA CCCAAGTTCA GGTGGGTTAG CTCACCCACC ACTTCCCGAG 
GCCAAGGGTT ACCCGAGCCC AGAGGTGATT CGGGAGTCCA GCAGCACCAC GGGCATGGTC 
GTAGGCATCG TTGCAGCCGC TGCACTCTGC ATCCTAATCC TGCTCTACGC CATGTACAAA 
TACAGAAACC GCGACGAAGG GTCTTACCAC GTGGACGAGA GTCGTAACTA CATTAGTAAT 
TCTGCCACGC AGCCCAATGG AGCTGCCGTC AAAGAAAAGC CCATCGGCGT GCCCAAAAAT 
AAAAAAGATA AAAAGAACAA GGACAAGGAG TACTACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306645.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304920.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa44656
Clone ID Related Accession (Same CDS sequence)	XM_009306645.2 , XM_009306645.3
Accession Version	XM_009306645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG AGAGAGAAGA ATATATGGCC 
ACATTCAAGG GTTCGGAGTA TTTCTGTTAC GATCTGTCCC CGAACCCCAT CCAGAGCAGT 
AGCGATGAGA TTACGCTCTC CTTCAAGACC CTGCAGCGCA ATGGACTGAT GCTTCACACC 
GGAAAGTCTG CAGACTACGT CAACCTGGCG CTGAAGAACG GAGCCGTCTC GCTGGTCATC 
AACCTGGGCT CTGGGGCCTT CGAGGCCCTG GTGGAGCCCG TCAATGGGAA ATTTAATGAC 
AATGCCTGGC ATGATGTGAA GGTTACCAGG AACCTTCGTC AGCACTCAGG CATTGGACAC 
GCTATGGTTA CAATATCCGT GGATGGGATT CTGACCACCA CTGGATATAC GCAAGAGGAC 
TACACCATGC TAGGCTCGGA TGACTTTTTC TATGTCGGCG GTAGTCCCAG CACTGCCGAT 
CTTCCTGGAT CACCTGTCAG CAACAACTTT ATGGGCTGCT TGAAAGAGGT TGTTTACAAA 
AACAACGATG TAAGACTTGA GCTGTCTCGA CTGGCCAAGC TGGGCGACCC AAAGATGAAG 
GTGAGCGGTG TGGTGGCCTT CAAATGTGAG AACGTGGCTA CTCTCGACCC TGTGACTTTT 
GAGACGCCTG AGTCATTCAT TACCCTTGAC AAGTGGTCCG CCAAAAAAGC CGGCTCCATC 
TCTTTCGACT TCCGCACCAC TGAACCCAAC GGCCTGCTCC TCTTCAGCCA TGGAAAACCC 
AAACCCCAGC AACAGAAAGA CCCTAAGAGC CCTAAAACAT TGAAGGTGGA TTTCTTTGCC 
ATTGAGATGC TGGACGGCCA CCTCTACCTT CTTCTCGACA TGGGCTCAGG CACCACCAAA 
ACTAGAGCTG TCAACAAGAA AGTCAATGAC GGAGAATGGT ACCACGTGGA CTTCCAGAGA 
GATGGAAGAT CAGGCACCAT TTCCGTCAAC TCGATTCGCA CTCCCTACAA CGCTCCTGGT 
GAGAGTGAGA TACTGGACCT GGACGATAAA CTGTACCTGG GCGGCCTGCC AGAGGACCGG 
GCCGGTCTGA TCTTCCCCAC GGAGGTGTGG ACCGCTCTCC TAAATTACGG CTATGTGGGC 
TGTGTGCGGG ATCTGTTCAT GGATGGCCAG AGCAAGGACA TTCGGCGGAT AGCAGAGGCT 
CAGAGAGCGG TCGGGGTGAA GCCGTCTTGC TCTAAAGAAC CCCCCAAACA GTGTCTGAGC 
AACCCCTGTC TGAACAGCGG CACCTGCAGA GAGGGCTGGA ACCGCTATGT GTGCGACTGC 
TCCGGGACGG GATATCTGGG ACGCTCCTGT GAGAGAGATG CCACGATCCT CAGTTACGAT 
GGTAGTAAGT TCATGAAGAT CCAGCTGCCG GTGGTGATGC ACACAGAAGC GGAGGACGTG 
TCCCTGCGCT TTCGTTCTCA ACGGGCCTAC GGTGTTCTAA TGGCGACCAC ATCGCAAAAC 
TCTGCTGATA CTCTGAGGCT GGAGCTGGAC GGAGGGCGGG TCCGACTCAC TGTCAATCTA 
GACTGTATCA GGATAAACTG TACTACCAGT AAAGGTCCAG AGACCATATT TTCGGGCCAG 
AACTTGAATG ACAACGAATG GCACACAGTT CGTGTCGTCA GAAGAGGCAA AAGTCTAAAG 
CTGATGGTGG ACGACTTGCA GCCCTCTGAA GGTCAAATCA CTGGGGACCA CACGCAGCTG 
GAGTTCCACA ATGTGGAAAC GGGCATCGTT ACCGAGAAGC GCTACATGCC CGCTGTGCCC 
TCCAACTTCA TCGGACACCT GCAGGGTTTG TCCTTCAATG GCATGTCCTA CATTGACTTG 
TGCAAGAACG GCGATATCGA CTACTGCGAA CTGAACGCCA TGATCGGCTA CAGGAGCATC 
GTGGCAGACC CAGTGACCTT CAAGTCTCGC TCCAGCTATG TGACTTTGCC CACCCTGCAG 
GCCTACTATT CGATGCACCT CTTCTTCCAG TTTAAGACTA CTTCTCCAGA TGGGCTCATC 
CTGTACAACC GAGGAGATGG CAATGACTTT ATAGTGGTAG AGCTGGTTAA AGGATATCTC 
CATTACGTGT CAGATCTGGG AAATGGAGCT CATCTCATCA AAGGCAATTC CAACACACCG 
CTAAATGACA ATCACTGGCA CAACGTGATG ATCTCGCGAG ATACAAACAA CCTCCATACT 
GTCAAAATAG ACACCAAAAT CACCACACAG ACTACCATGG GAGCCAAGAA CCTGGATCTG 
AAAGGTGATC TGTATGTTGG AGGTGTGGCT AAAGATATGT ATAAGGACTT GCCAAAGCTG 
GTGCATTCAA AAGAGGGCTT CCAGGGCTGC CTGGCATCTG TGGACCTGAA CGGCCGCTTG 
CCAGACCTGC AGTCAGACGC ACTGTCCACC GCCGGCCAGG TGGAGAGAGG CTGCGAAGGT 
GAGGAAAACG TTGCATTGCT GAAAGCTGAC TTGCAAGGCC CCAGCACTAC CTGTCAGGAG 
GACTCCTGCT CCAATCAGGG CGTGTGTCTG CAGCAGTGGG AGGGGTTTAG TTGCGACTGC 
AGCATGACAT CATATGGAGG GCCACTCTGC AATGACCCGG GAACCACATA CATATTCGGA 
CGTGATGGAG GGCTTATTGT GTACACATGG CCACCCAACG ACAGACCGAG CACACGAGCT 
GACCGACTGG CTGTCGGTTT TAGTACCCAG CAGAAGGATG CAGTGCTGGT TAGGGTGGAC 
AGCTCTTCTG GCCTGGGAGA CTACCTTCAG CTTCAGATTG AAAGAGGCAA TATCAAAGTG 
GTGTTCAATG TGGGCACCGA TGACATAAAC ATTGAAGAAA CTTCAAAGTT TGTAAATGAT 
GGAAAGTACC ACATAGTTCG CTTTACGAGA AGTGGTGGTA ACGCCACCTT GCAGGTGGAT 
GATCTGCCAG TCATTGAGCG CTACCCATCA GGCAACATTG ATAACGAACG CCTGGCGATT 
GCTAGACAGC GAATCCCATA TCGGCTTGGT CGAGTAGTTG ACGATTGGCT ACTCGACAAA 
GGTCGGCAGC TCACCATCTT CAACAGTCAG ACCACCATAA AGATTGGAGG CTGGGAGAAG 
GGCAGCCGGC CCTTCCAGGG TCAGCTCTCA GGGCTTTACT ACAATGGGCT AAAAGTACTC 
AACATGGCCG CTGAGGGTGA CCCTAATGTG CGTGTGGAGG GTAGTGCCCG GCTGGTAGGA 
GACATGCCCT CCTCTTCTAT CACGCCACAG TCCAGCGTCT CTGCTGCGGG AAATCGTTCA 
GAAACGTCAC CCTCCATCAC TGACATCACC ACTACAACGG CGTCCAACCG CCAGGGCAAG 
CAGACCACAA CTCCACAGGA CGATCTTCTC GTAGCGTCTG CAGAATGCCC CAGCGATGAT 
GAGGACATTG ACCCTTGCGA CCCAAGTTCA GGTGGGTTAG CTCACCCACC ACTTCCCGAG 
GCCAAGGGTT ACCCGAGCCC AGAGGTGATT CGGGAGTCCA GCAGCACCAC GGGCATGGTC 
GTAGGCATCG TTGCAGCCGC TGCACTCTGC ATCCTAATCC TGCTCTACGC CATGTACAAA 
TACAGAAACC GCGACGAAGG GTCTTACCAC GTGGACGAGA GTCGTAACTA CATTAGTAAT 
TCTGCCACGC AGCCCAATGG AGCTGCCGTC AAAGAAAAGC CCATCGGCGT GCCCAAAAAT 
AAAAAAGATA AAAAGAACAA GGACAAGGAG TACTACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304920.1
CDS	986..5467
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306645.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306645.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG AGAGAGAAGA ATATATGGCC 
ACATTCAAGG GTTCGGAGTA TTTCTGTTAC GATCTGTCCC CGAACCCCAT CCAGAGCAGT 
AGCGATGAGA TTACGCTCTC CTTCAAGACC CTGCAGCGCA ATGGACTGAT GCTTCACACC 
GGAAAGTCTG CAGACTACGT CAACCTGGCG CTGAAGAACG GAGCCGTCTC GCTGGTCATC 
AACCTGGGCT CTGGGGCCTT CGAGGCCCTG GTGGAGCCCG TCAATGGGAA ATTTAATGAC 
AATGCCTGGC ATGATGTGAA GGTTACCAGG AACCTTCGTC AGCACTCAGG CATTGGACAC 
GCTATGGTTA CAATATCCGT GGATGGGATT CTGACCACCA CTGGATATAC GCAAGAGGAC 
TACACCATGC TAGGCTCGGA TGACTTTTTC TATGTCGGCG GTAGTCCCAG CACTGCCGAT 
CTTCCTGGAT CACCTGTCAG CAACAACTTT ATGGGCTGCT TGAAAGAGGT TGTTTACAAA 
AACAACGATG TAAGACTTGA GCTGTCTCGA CTGGCCAAGC TGGGCGACCC AAAGATGAAG 
GTGAGCGGTG TGGTGGCCTT CAAATGTGAG AACGTGGCTA CTCTCGACCC TGTGACTTTT 
GAGACGCCTG AGTCATTCAT TACCCTTGAC AAGTGGTCCG CCAAAAAAGC CGGCTCCATC 
TCTTTCGACT TCCGCACCAC TGAACCCAAC GGCCTGCTCC TCTTCAGCCA TGGAAAACCC 
AAACCCCAGC AACAGAAAGA CCCTAAGAGC CCTAAAACAT TGAAGGTGGA TTTCTTTGCC 
ATTGAGATGC TGGACGGCCA CCTCTACCTT CTTCTCGACA TGGGCTCAGG CACCACCAAA 
ACTAGAGCTG TCAACAAGAA AGTCAATGAC GGAGAATGGT ACCACGTGGA CTTCCAGAGA 
GATGGAAGAT CAGGCACCAT TTCCGTCAAC TCGATTCGCA CTCCCTACAA CGCTCCTGGT 
GAGAGTGAGA TACTGGACCT GGACGATAAA CTGTACCTGG GCGGCCTGCC AGAGGACCGG 
GCCGGTCTGA TCTTCCCCAC GGAGGTGTGG ACCGCTCTCC TAAATTACGG CTATGTGGGC 
TGTGTGCGGG ATCTGTTCAT GGATGGCCAG AGCAAGGACA TTCGGCGGAT AGCAGAGGCT 
CAGAGAGCGG TCGGGGTGAA GCCGTCTTGC TCTAAAGAAC CCCCCAAACA GTGTCTGAGC 
AACCCCTGTC TGAACAGCGG CACCTGCAGA GAGGGCTGGA ACCGCTATGT GTGCGACTGC 
TCCGGGACGG GATATCTGGG ACGCTCCTGT GAGAGAGATG CCACGATCCT CAGTTACGAT 
GGTAGTAAGT TCATGAAGAT CCAGCTGCCG GTGGTGATGC ACACAGAAGC GGAGGACGTG 
TCCCTGCGCT TTCGTTCTCA ACGGGCCTAC GGTGTTCTAA TGGCGACCAC ATCGCAAAAC 
TCTGCTGATA CTCTGAGGCT GGAGCTGGAC GGAGGGCGGG TCCGACTCAC TGTCAATCTA 
GACTGTATCA GGATAAACTG TACTACCAGT AAAGGTCCAG AGACCATATT TTCGGGCCAG 
AACTTGAATG ACAACGAATG GCACACAGTT CGTGTCGTCA GAAGAGGCAA AAGTCTAAAG 
CTGATGGTGG ACGACTTGCA GCCCTCTGAA GGTCAAATCA CTGGGGACCA CACGCAGCTG 
GAGTTCCACA ATGTGGAAAC GGGCATCGTT ACCGAGAAGC GCTACATGCC CGCTGTGCCC 
TCCAACTTCA TCGGACACCT GCAGGGTTTG TCCTTCAATG GCATGTCCTA CATTGACTTG 
TGCAAGAACG GCGATATCGA CTACTGCGAA CTGAACGCCA TGATCGGCTA CAGGAGCATC 
GTGGCAGACC CAGTGACCTT CAAGTCTCGC TCCAGCTATG TGACTTTGCC CACCCTGCAG 
GCCTACTATT CGATGCACCT CTTCTTCCAG TTTAAGACTA CTTCTCCAGA TGGGCTCATC 
CTGTACAACC GAGGAGATGG CAATGACTTT ATAGTGGTAG AGCTGGTTAA AGGATATCTC 
CATTACGTGT CAGATCTGGG AAATGGAGCT CATCTCATCA AAGGCAATTC CAACACACCG 
CTAAATGACA ATCACTGGCA CAACGTGATG ATCTCGCGAG ATACAAACAA CCTCCATACT 
GTCAAAATAG ACACCAAAAT CACCACACAG ACTACCATGG GAGCCAAGAA CCTGGATCTG 
AAAGGTGATC TGTATGTTGG AGGTGTGGCT AAAGATATGT ATAAGGACTT GCCAAAGCTG 
GTGCATTCAA AAGAGGGCTT CCAGGGCTGC CTGGCATCTG TGGACCTGAA CGGCCGCTTG 
CCAGACCTGC AGTCAGACGC ACTGTCCACC GCCGGCCAGG TGGAGAGAGG CTGCGAAGGT 
GAGGAAAACG TTGCATTGCT GAAAGCTGAC TTGCAAGGCC CCAGCACTAC CTGTCAGGAG 
GACTCCTGCT CCAATCAGGG CGTGTGTCTG CAGCAGTGGG AGGGGTTTAG TTGCGACTGC 
AGCATGACAT CATATGGAGG GCCACTCTGC AATGACCCGG GAACCACATA CATATTCGGA 
CGTGATGGAG GGCTTATTGT GTACACATGG CCACCCAACG ACAGACCGAG CACACGAGCT 
GACCGACTGG CTGTCGGTTT TAGTACCCAG CAGAAGGATG CAGTGCTGGT TAGGGTGGAC 
AGCTCTTCTG GCCTGGGAGA CTACCTTCAG CTTCAGATTG AAAGAGGCAA TATCAAAGTG 
GTGTTCAATG TGGGCACCGA TGACATAAAC ATTGAAGAAA CTTCAAAGTT TGTAAATGAT 
GGAAAGTACC ACATAGTTCG CTTTACGAGA AGTGGTGGTA ACGCCACCTT GCAGGTGGAT 
GATCTGCCAG TCATTGAGCG CTACCCATCA GGCAACATTG ATAACGAACG CCTGGCGATT 
GCTAGACAGC GAATCCCATA TCGGCTTGGT CGAGTAGTTG ACGATTGGCT ACTCGACAAA 
GGTCGGCAGC TCACCATCTT CAACAGTCAG ACCACCATAA AGATTGGAGG CTGGGAGAAG 
GGCAGCCGGC CCTTCCAGGG TCAGCTCTCA GGGCTTTACT ACAATGGGCT AAAAGTACTC 
AACATGGCCG CTGAGGGTGA CCCTAATGTG CGTGTGGAGG GTAGTGCCCG GCTGGTAGGA 
GACATGCCCT CCTCTTCTAT CACGCCACAG TCCAGCGTCT CTGCTGCGGG AAATCGTTCA 
GAAACGTCAC CCTCCATCAC TGACATCACC ACTACAACGG CGTCCAACCG CCAGGGCAAG 
CAGACCACAA CTCCACAGGA CGATCTTCTC GTAGCGTCTG CAGAATGCCC CAGCGATGAT 
GAGGACATTG ACCCTTGCGA CCCAAGTTCA GGTGGGTTAG CTCACCCACC ACTTCCCGAG 
GCCAAGGGTT ACCCGAGCCC AGAGGTGATT CGGGAGTCCA GCAGCACCAC GGGCATGGTC 
GTAGGCATCG TTGCAGCCGC TGCACTCTGC ATCCTAATCC TGCTCTACGC CATGTACAAA 
TACAGAAACC GCGACGAAGG GTCTTACCAC GTGGACGAGA GTCGTAACTA CATTAGTAAT 
TCTGCCACGC AGCCCAATGG AGCTGCCGTC AAAGAAAAGC CCATCGGCGT GCCCAAAAAT 
AAAAAAGATA AAAAGAACAA GGACAAGGAG TACTACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306646.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304921.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X6, mRNA.

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CloneID	ODa44657
Clone ID Related Accession (Same CDS sequence)	XM_009306646.2 , XM_009306646.3
Accession Version	XM_009306646.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGGCCTAT 
GTAGGAGGTC TTGCGCACCT GATGATGGGC GACCAAGAGA GAGAAGAATA TATGGCCACA 
TTCAAGGGTT CGGAGTATTT CTGTTACGAT CTGTCCCCGA ACCCCATCCA GAGCAGTAGC 
GATGAGATTA CGCTCTCCTT CAAGACCCTG CAGCGCAATG GACTGATGCT TCACACCGGA 
AAGTCTGCAG ACTACGTCAA CCTGGCGCTG AAGAACGGAG CCGTCTCGCT GGTCATCAAC 
CTGGGCTCTG GGGCCTTCGA GGCCCTGGTG GAGCCCGTCA ATGGGAAATT TAATGACAAT 
GCCTGGCATG ATGTGAAGGT TACCAGGAAC CTTCGTCAGC ACTCAGGCAT TGGACACGCT 
ATGGTTACAA TATCCGTGGA TGGGATTCTG ACCACCACTG GATATACGCA AGAGGACTAC 
ACCATGCTAG GCTCGGATGA CTTTTTCTAT GTCGGCGGTA GTCCCAGCAC TGCCGATCTT 
CCTGGATCAC CTGTCAGCAA CAACTTTATG GGCTGCTTGA AAGAGGTTGT TTACAAAAAC 
AACGATGTAA GACTTGAGCT GTCTCGACTG GCCAAGCTGG GCGACCCAAA GATGAAGGTG 
AGCGGTGTGG TGGCCTTCAA ATGTGAGAAC GTGGCTACTC TCGACCCTGT GACTTTTGAG 
ACGCCTGAGT CATTCATTAC CCTTGACAAG TGGTCCGCCA AAAAAGCCGG CTCCATCTCT 
TTCGACTTCC GCACCACTGA ACCCAACGGC CTGCTCCTCT TCAGCCATGG AAAACCCAAA 
CCCCAGCAAC AGAAAGACCC TAAGAGCCCT AAAACATTGA AGGTGGATTT CTTTGCCATT 
GAGATGCTGG ACGGCCACCT CTACCTTCTT CTCGACATGG GCTCAGGCAC CACCAAAACT 
AGAGCTGTCA ACAAGAAAGT CAATGACGGA GAATGGTACC ACGTGGACTT CCAGAGAGAT 
GGAAGATCAG GCACCATTTC CGTCAACTCG ATTCGCACTC CCTACAACGC TCCTGGTGAG 
AGTGAGATAC TGGACCTGGA CGATAAACTG TACCTGGGCG GCCTGCCAGA GGACCGGGCC 
GGTCTGATCT TCCCCACGGA GGTGTGGACC GCTCTCCTAA ATTACGGCTA TGTGGGCTGT 
GTGCGGGATC TGTTCATGGA TGGCCAGAGC AAGGACATTC GGCGGATAGC AGAGGCTCAG 
AGAGCGGTCG GGGTGAAGCC GTCTTGCTCT AAAGAACCCC CCAAACAGTG TCTGAGCAAC 
CCCTGTCTGA ACAGCGGCAC CTGCAGAGAG GGCTGGAACC GCTATGTGTG CGACTGCTCC 
GGGACGGGAT ATCTGGGACG CTCCTGTGAG AGAGATGCCA CGATCCTCAG TTACGATGGT 
AGTAAGTTCA TGAAGATCCA GCTGCCGGTG GTGATGCACA CAGAAGCGGA GGACGTGTCC 
CTGCGCTTTC GTTCTCAACG GGCCTACGGT GTTCTAATGG CGACCACATC GCAAAACTCT 
GCTGATACTC TGAGGCTGGA GCTGGACGGA GGGCGGGTCC GACTCACTGT CAATCTAGAC 
TGTATCAGGA TAAACTGTAC TACCAGTAAA GGTCCAGAGA CCATATTTTC GGGCCAGAAC 
TTGAATGACA ACGAATGGCA CACAGTTCGT GTCGTCAGAA GAGGCAAAAG TCTAAAGCTG 
ATGGTGGACG ACTTGCAGCC CTCTGAAGGT CAAATCACTG GGGACCACAC GCAGCTGGAG 
TTCCACAATG TGGAAACGGG CATCGTTACC GAGAAGCGCT ACATGCCCGC TGTGCCCTCC 
AACTTCATCG GACACCTGCA GGGTTTGTCC TTCAATGGCA TGTCCTACAT TGACTTGTGC 
AAGAACGGCG ATATCGACTA CTGCGAACTG AACGCCATGA TCGGCTACAG GAGCATCGTG 
GCAGACCCAG TGACCTTCAA GTCTCGCTCC AGCTATGTGA CTTTGCCCAC CCTGCAGGCC 
TACTATTCGA TGCACCTCTT CTTCCAGTTT AAGACTACTT CTCCAGATGG GCTCATCCTG 
TACAACCGAG GAGATGGCAA TGACTTTATA GTGGTAGAGC TGGTTAAAGG ATATCTCCAT 
TACGTGTCAG ATCTGGGAAA TGGAGCTCAT CTCATCAAAG GCAATTCCAA CACACCGCTA 
AATGACAATC ACTGGCACAA CGTGATGATC TCGCGAGATA CAAACAACCT CCATACTGTC 
AAAATAGACA CCAAAATCAC CACACAGACT ACCATGGGAG CCAAGAACCT GGATCTGAAA 
GGTGATCTGT ATGTTGGAGG TGTGGCTAAA GATATGTATA AGGACTTGCC AAAGCTGGTG 
CATTCAAAAG AGGGCTTCCA GGGCTGCCTG GCATCTGTGG ACCTGAACGG CCGCTTGCCA 
GACCTGCAGT CAGACGCACT GTCCACCGCC GGCCAGGTGG AGAGAGGCTG CGAAGGTGAG 
GAAAACGTTG CATTGCTGAA AGCTGACTTG CAAGGCCCCA GCACTACCTG TCAGGAGGAC 
TCCTGCTCCA ATCAGGGCGT GTGTCTGCAG CAGTGGGAGG GGTTTAGTTG CGACTGCAGC 
ATGACATCAT ATGGAGGGCC ACTCTGCAAT GACCCGGGAA CCACATACAT ATTCGGACGT 
GATGGAGGGC TTATTGTGTA CACATGGCCA CCCAACGACA GACCGAGCAC ACGAGCTGAC 
CGACTGGCTG TCGGTTTTAG TACCCAGCAG AAGGATGCAG TGCTGGTTAG GGTGGACAGC 
TCTTCTGGCC TGGGAGACTA CCTTCAGCTT CAGATTGAAA GAGGCAATAT CAAAGTGGTG 
TTCAATGTGG GCACCGATGA CATAAACATT GAAGAAACTT CAAAGTTTGT AAATGATGGA 
AAGTACCACA TAGTTCGCTT TACGAGAAGT GGTGGTAACG CCACCTTGCA GGTGGATGAT 
CTGCCAGTCA TTGAGCGCTA CCCATCAGGC AACATTGATA ACGAACGCCT GGCGATTGCT 
AGACAGCGAA TCCCATATCG GCTTGGTCGA GTAGTTGACG ATTGGCTACT CGACAAAGGT 
CGGCAGCTCA CCATCTTCAA CAGTCAGACC ACCATAAAGA TTGGAGGCTG GGAGAAGGGC 
AGCCGGCCCT TCCAGGGTCA GCTCTCAGGG CTTTACTACA ATGGGCTAAA AGTACTCAAC 
ATGGCCGCTG AGGGTGACCC TAATGTGCGT GTGGAGGGTA GTGCCCGGCT GGTAGGAGAC 
ATGCCCTCCT CTTCTATCAC GCCACAGTCC AGCGTCTCTG CTGCGGGAAA TCGTTCAGAA 
ACGTCACCCT CCATCACTGA CATCACCACT ACAACGGCGT CCAACCGCCA GGGCAAGCAG 
ACCACAACTC CACAGGACGA TCTTCTCGTA GCGTCTGCAG AATGCCCCAG CGATGATGAG 
GACATTGACC CTTGCGACCC AAGTTCAGGT GGGTTAGCTC ACCCACCACT TCCCGAGGCC 
AAGGGTTACC CGAGCCCAGA GGTGATTCGG GAGTCCAGCA GCACCACGGG CATGGTCGTA 
GGCATCGTTG CAGCCGCTGC ACTCTGCATC CTAATCCTGC TCTACGCCAT GTACAAATAC 
AGAAACCGCG ACGAAGGGTC TTACCACGTG GACGAGAGTC GTAACTACAT TAGTAATTCT 
GCCACGCAGC CCAATGGAGC TGCCGTCAAA GAAAAGCCCA TCGGCGTGCC CAAAAATAAA 
AAAGATAAAA AGAACAAGGA CAAGGAGTAC TACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304921.1
CDS	983..5461
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEAYVGGLAHLMMGDQEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306646.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306646.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGGCCTAT 
GTAGGAGGTC TTGCGCACCT GATGATGGGC GACCAAGAGA GAGAAGAATA TATGGCCACA 
TTCAAGGGTT CGGAGTATTT CTGTTACGAT CTGTCCCCGA ACCCCATCCA GAGCAGTAGC 
GATGAGATTA CGCTCTCCTT CAAGACCCTG CAGCGCAATG GACTGATGCT TCACACCGGA 
AAGTCTGCAG ACTACGTCAA CCTGGCGCTG AAGAACGGAG CCGTCTCGCT GGTCATCAAC 
CTGGGCTCTG GGGCCTTCGA GGCCCTGGTG GAGCCCGTCA ATGGGAAATT TAATGACAAT 
GCCTGGCATG ATGTGAAGGT TACCAGGAAC CTTCGTCAGC ACTCAGGCAT TGGACACGCT 
ATGGTTACAA TATCCGTGGA TGGGATTCTG ACCACCACTG GATATACGCA AGAGGACTAC 
ACCATGCTAG GCTCGGATGA CTTTTTCTAT GTCGGCGGTA GTCCCAGCAC TGCCGATCTT 
CCTGGATCAC CTGTCAGCAA CAACTTTATG GGCTGCTTGA AAGAGGTTGT TTACAAAAAC 
AACGATGTAA GACTTGAGCT GTCTCGACTG GCCAAGCTGG GCGACCCAAA GATGAAGGTG 
AGCGGTGTGG TGGCCTTCAA ATGTGAGAAC GTGGCTACTC TCGACCCTGT GACTTTTGAG 
ACGCCTGAGT CATTCATTAC CCTTGACAAG TGGTCCGCCA AAAAAGCCGG CTCCATCTCT 
TTCGACTTCC GCACCACTGA ACCCAACGGC CTGCTCCTCT TCAGCCATGG AAAACCCAAA 
CCCCAGCAAC AGAAAGACCC TAAGAGCCCT AAAACATTGA AGGTGGATTT CTTTGCCATT 
GAGATGCTGG ACGGCCACCT CTACCTTCTT CTCGACATGG GCTCAGGCAC CACCAAAACT 
AGAGCTGTCA ACAAGAAAGT CAATGACGGA GAATGGTACC ACGTGGACTT CCAGAGAGAT 
GGAAGATCAG GCACCATTTC CGTCAACTCG ATTCGCACTC CCTACAACGC TCCTGGTGAG 
AGTGAGATAC TGGACCTGGA CGATAAACTG TACCTGGGCG GCCTGCCAGA GGACCGGGCC 
GGTCTGATCT TCCCCACGGA GGTGTGGACC GCTCTCCTAA ATTACGGCTA TGTGGGCTGT 
GTGCGGGATC TGTTCATGGA TGGCCAGAGC AAGGACATTC GGCGGATAGC AGAGGCTCAG 
AGAGCGGTCG GGGTGAAGCC GTCTTGCTCT AAAGAACCCC CCAAACAGTG TCTGAGCAAC 
CCCTGTCTGA ACAGCGGCAC CTGCAGAGAG GGCTGGAACC GCTATGTGTG CGACTGCTCC 
GGGACGGGAT ATCTGGGACG CTCCTGTGAG AGAGATGCCA CGATCCTCAG TTACGATGGT 
AGTAAGTTCA TGAAGATCCA GCTGCCGGTG GTGATGCACA CAGAAGCGGA GGACGTGTCC 
CTGCGCTTTC GTTCTCAACG GGCCTACGGT GTTCTAATGG CGACCACATC GCAAAACTCT 
GCTGATACTC TGAGGCTGGA GCTGGACGGA GGGCGGGTCC GACTCACTGT CAATCTAGAC 
TGTATCAGGA TAAACTGTAC TACCAGTAAA GGTCCAGAGA CCATATTTTC GGGCCAGAAC 
TTGAATGACA ACGAATGGCA CACAGTTCGT GTCGTCAGAA GAGGCAAAAG TCTAAAGCTG 
ATGGTGGACG ACTTGCAGCC CTCTGAAGGT CAAATCACTG GGGACCACAC GCAGCTGGAG 
TTCCACAATG TGGAAACGGG CATCGTTACC GAGAAGCGCT ACATGCCCGC TGTGCCCTCC 
AACTTCATCG GACACCTGCA GGGTTTGTCC TTCAATGGCA TGTCCTACAT TGACTTGTGC 
AAGAACGGCG ATATCGACTA CTGCGAACTG AACGCCATGA TCGGCTACAG GAGCATCGTG 
GCAGACCCAG TGACCTTCAA GTCTCGCTCC AGCTATGTGA CTTTGCCCAC CCTGCAGGCC 
TACTATTCGA TGCACCTCTT CTTCCAGTTT AAGACTACTT CTCCAGATGG GCTCATCCTG 
TACAACCGAG GAGATGGCAA TGACTTTATA GTGGTAGAGC TGGTTAAAGG ATATCTCCAT 
TACGTGTCAG ATCTGGGAAA TGGAGCTCAT CTCATCAAAG GCAATTCCAA CACACCGCTA 
AATGACAATC ACTGGCACAA CGTGATGATC TCGCGAGATA CAAACAACCT CCATACTGTC 
AAAATAGACA CCAAAATCAC CACACAGACT ACCATGGGAG CCAAGAACCT GGATCTGAAA 
GGTGATCTGT ATGTTGGAGG TGTGGCTAAA GATATGTATA AGGACTTGCC AAAGCTGGTG 
CATTCAAAAG AGGGCTTCCA GGGCTGCCTG GCATCTGTGG ACCTGAACGG CCGCTTGCCA 
GACCTGCAGT CAGACGCACT GTCCACCGCC GGCCAGGTGG AGAGAGGCTG CGAAGGTGAG 
GAAAACGTTG CATTGCTGAA AGCTGACTTG CAAGGCCCCA GCACTACCTG TCAGGAGGAC 
TCCTGCTCCA ATCAGGGCGT GTGTCTGCAG CAGTGGGAGG GGTTTAGTTG CGACTGCAGC 
ATGACATCAT ATGGAGGGCC ACTCTGCAAT GACCCGGGAA CCACATACAT ATTCGGACGT 
GATGGAGGGC TTATTGTGTA CACATGGCCA CCCAACGACA GACCGAGCAC ACGAGCTGAC 
CGACTGGCTG TCGGTTTTAG TACCCAGCAG AAGGATGCAG TGCTGGTTAG GGTGGACAGC 
TCTTCTGGCC TGGGAGACTA CCTTCAGCTT CAGATTGAAA GAGGCAATAT CAAAGTGGTG 
TTCAATGTGG GCACCGATGA CATAAACATT GAAGAAACTT CAAAGTTTGT AAATGATGGA 
AAGTACCACA TAGTTCGCTT TACGAGAAGT GGTGGTAACG CCACCTTGCA GGTGGATGAT 
CTGCCAGTCA TTGAGCGCTA CCCATCAGGC AACATTGATA ACGAACGCCT GGCGATTGCT 
AGACAGCGAA TCCCATATCG GCTTGGTCGA GTAGTTGACG ATTGGCTACT CGACAAAGGT 
CGGCAGCTCA CCATCTTCAA CAGTCAGACC ACCATAAAGA TTGGAGGCTG GGAGAAGGGC 
AGCCGGCCCT TCCAGGGTCA GCTCTCAGGG CTTTACTACA ATGGGCTAAA AGTACTCAAC 
ATGGCCGCTG AGGGTGACCC TAATGTGCGT GTGGAGGGTA GTGCCCGGCT GGTAGGAGAC 
ATGCCCTCCT CTTCTATCAC GCCACAGTCC AGCGTCTCTG CTGCGGGAAA TCGTTCAGAA 
ACGTCACCCT CCATCACTGA CATCACCACT ACAACGGCGT CCAACCGCCA GGGCAAGCAG 
ACCACAACTC CACAGGACGA TCTTCTCGTA GCGTCTGCAG AATGCCCCAG CGATGATGAG 
GACATTGACC CTTGCGACCC AAGTTCAGGT GGGTTAGCTC ACCCACCACT TCCCGAGGCC 
AAGGGTTACC CGAGCCCAGA GGTGATTCGG GAGTCCAGCA GCACCACGGG CATGGTCGTA 
GGCATCGTTG CAGCCGCTGC ACTCTGCATC CTAATCCTGC TCTACGCCAT GTACAAATAC 
AGAAACCGCG ACGAAGGGTC TTACCACGTG GACGAGAGTC GTAACTACAT TAGTAATTCT 
GCCACGCAGC CCAATGGAGC TGCCGTCAAA GAAAAGCCCA TCGGCGTGCC CAAAAATAAA 
AAAGATAAAA AGAACAAGGA CAAGGAGTAC TACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306646.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304921.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X6, mRNA.

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CloneID	ODa44657
Clone ID Related Accession (Same CDS sequence)	XM_009306646.2 , XM_009306646.3
Accession Version	XM_009306646.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306646.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGGCCTAT 
GTAGGAGGTC TTGCGCACCT GATGATGGGC GACCAAGAGA GAGAAGAATA TATGGCCACA 
TTCAAGGGTT CGGAGTATTT CTGTTACGAT CTGTCCCCGA ACCCCATCCA GAGCAGTAGC 
GATGAGATTA CGCTCTCCTT CAAGACCCTG CAGCGCAATG GACTGATGCT TCACACCGGA 
AAGTCTGCAG ACTACGTCAA CCTGGCGCTG AAGAACGGAG CCGTCTCGCT GGTCATCAAC 
CTGGGCTCTG GGGCCTTCGA GGCCCTGGTG GAGCCCGTCA ATGGGAAATT TAATGACAAT 
GCCTGGCATG ATGTGAAGGT TACCAGGAAC CTTCGTCAGC ACTCAGGCAT TGGACACGCT 
ATGGTTACAA TATCCGTGGA TGGGATTCTG ACCACCACTG GATATACGCA AGAGGACTAC 
ACCATGCTAG GCTCGGATGA CTTTTTCTAT GTCGGCGGTA GTCCCAGCAC TGCCGATCTT 
CCTGGATCAC CTGTCAGCAA CAACTTTATG GGCTGCTTGA AAGAGGTTGT TTACAAAAAC 
AACGATGTAA GACTTGAGCT GTCTCGACTG GCCAAGCTGG GCGACCCAAA GATGAAGGTG 
AGCGGTGTGG TGGCCTTCAA ATGTGAGAAC GTGGCTACTC TCGACCCTGT GACTTTTGAG 
ACGCCTGAGT CATTCATTAC CCTTGACAAG TGGTCCGCCA AAAAAGCCGG CTCCATCTCT 
TTCGACTTCC GCACCACTGA ACCCAACGGC CTGCTCCTCT TCAGCCATGG AAAACCCAAA 
CCCCAGCAAC AGAAAGACCC TAAGAGCCCT AAAACATTGA AGGTGGATTT CTTTGCCATT 
GAGATGCTGG ACGGCCACCT CTACCTTCTT CTCGACATGG GCTCAGGCAC CACCAAAACT 
AGAGCTGTCA ACAAGAAAGT CAATGACGGA GAATGGTACC ACGTGGACTT CCAGAGAGAT 
GGAAGATCAG GCACCATTTC CGTCAACTCG ATTCGCACTC CCTACAACGC TCCTGGTGAG 
AGTGAGATAC TGGACCTGGA CGATAAACTG TACCTGGGCG GCCTGCCAGA GGACCGGGCC 
GGTCTGATCT TCCCCACGGA GGTGTGGACC GCTCTCCTAA ATTACGGCTA TGTGGGCTGT 
GTGCGGGATC TGTTCATGGA TGGCCAGAGC AAGGACATTC GGCGGATAGC AGAGGCTCAG 
AGAGCGGTCG GGGTGAAGCC GTCTTGCTCT AAAGAACCCC CCAAACAGTG TCTGAGCAAC 
CCCTGTCTGA ACAGCGGCAC CTGCAGAGAG GGCTGGAACC GCTATGTGTG CGACTGCTCC 
GGGACGGGAT ATCTGGGACG CTCCTGTGAG AGAGATGCCA CGATCCTCAG TTACGATGGT 
AGTAAGTTCA TGAAGATCCA GCTGCCGGTG GTGATGCACA CAGAAGCGGA GGACGTGTCC 
CTGCGCTTTC GTTCTCAACG GGCCTACGGT GTTCTAATGG CGACCACATC GCAAAACTCT 
GCTGATACTC TGAGGCTGGA GCTGGACGGA GGGCGGGTCC GACTCACTGT CAATCTAGAC 
TGTATCAGGA TAAACTGTAC TACCAGTAAA GGTCCAGAGA CCATATTTTC GGGCCAGAAC 
TTGAATGACA ACGAATGGCA CACAGTTCGT GTCGTCAGAA GAGGCAAAAG TCTAAAGCTG 
ATGGTGGACG ACTTGCAGCC CTCTGAAGGT CAAATCACTG GGGACCACAC GCAGCTGGAG 
TTCCACAATG TGGAAACGGG CATCGTTACC GAGAAGCGCT ACATGCCCGC TGTGCCCTCC 
AACTTCATCG GACACCTGCA GGGTTTGTCC TTCAATGGCA TGTCCTACAT TGACTTGTGC 
AAGAACGGCG ATATCGACTA CTGCGAACTG AACGCCATGA TCGGCTACAG GAGCATCGTG 
GCAGACCCAG TGACCTTCAA GTCTCGCTCC AGCTATGTGA CTTTGCCCAC CCTGCAGGCC 
TACTATTCGA TGCACCTCTT CTTCCAGTTT AAGACTACTT CTCCAGATGG GCTCATCCTG 
TACAACCGAG GAGATGGCAA TGACTTTATA GTGGTAGAGC TGGTTAAAGG ATATCTCCAT 
TACGTGTCAG ATCTGGGAAA TGGAGCTCAT CTCATCAAAG GCAATTCCAA CACACCGCTA 
AATGACAATC ACTGGCACAA CGTGATGATC TCGCGAGATA CAAACAACCT CCATACTGTC 
AAAATAGACA CCAAAATCAC CACACAGACT ACCATGGGAG CCAAGAACCT GGATCTGAAA 
GGTGATCTGT ATGTTGGAGG TGTGGCTAAA GATATGTATA AGGACTTGCC AAAGCTGGTG 
CATTCAAAAG AGGGCTTCCA GGGCTGCCTG GCATCTGTGG ACCTGAACGG CCGCTTGCCA 
GACCTGCAGT CAGACGCACT GTCCACCGCC GGCCAGGTGG AGAGAGGCTG CGAAGGTGAG 
GAAAACGTTG CATTGCTGAA AGCTGACTTG CAAGGCCCCA GCACTACCTG TCAGGAGGAC 
TCCTGCTCCA ATCAGGGCGT GTGTCTGCAG CAGTGGGAGG GGTTTAGTTG CGACTGCAGC 
ATGACATCAT ATGGAGGGCC ACTCTGCAAT GACCCGGGAA CCACATACAT ATTCGGACGT 
GATGGAGGGC TTATTGTGTA CACATGGCCA CCCAACGACA GACCGAGCAC ACGAGCTGAC 
CGACTGGCTG TCGGTTTTAG TACCCAGCAG AAGGATGCAG TGCTGGTTAG GGTGGACAGC 
TCTTCTGGCC TGGGAGACTA CCTTCAGCTT CAGATTGAAA GAGGCAATAT CAAAGTGGTG 
TTCAATGTGG GCACCGATGA CATAAACATT GAAGAAACTT CAAAGTTTGT AAATGATGGA 
AAGTACCACA TAGTTCGCTT TACGAGAAGT GGTGGTAACG CCACCTTGCA GGTGGATGAT 
CTGCCAGTCA TTGAGCGCTA CCCATCAGGC AACATTGATA ACGAACGCCT GGCGATTGCT 
AGACAGCGAA TCCCATATCG GCTTGGTCGA GTAGTTGACG ATTGGCTACT CGACAAAGGT 
CGGCAGCTCA CCATCTTCAA CAGTCAGACC ACCATAAAGA TTGGAGGCTG GGAGAAGGGC 
AGCCGGCCCT TCCAGGGTCA GCTCTCAGGG CTTTACTACA ATGGGCTAAA AGTACTCAAC 
ATGGCCGCTG AGGGTGACCC TAATGTGCGT GTGGAGGGTA GTGCCCGGCT GGTAGGAGAC 
ATGCCCTCCT CTTCTATCAC GCCACAGTCC AGCGTCTCTG CTGCGGGAAA TCGTTCAGAA 
ACGTCACCCT CCATCACTGA CATCACCACT ACAACGGCGT CCAACCGCCA GGGCAAGCAG 
ACCACAACTC CACAGGACGA TCTTCTCGTA GCGTCTGCAG AATGCCCCAG CGATGATGAG 
GACATTGACC CTTGCGACCC AAGTTCAGGT GGGTTAGCTC ACCCACCACT TCCCGAGGCC 
AAGGGTTACC CGAGCCCAGA GGTGATTCGG GAGTCCAGCA GCACCACGGG CATGGTCGTA 
GGCATCGTTG CAGCCGCTGC ACTCTGCATC CTAATCCTGC TCTACGCCAT GTACAAATAC 
AGAAACCGCG ACGAAGGGTC TTACCACGTG GACGAGAGTC GTAACTACAT TAGTAATTCT 
GCCACGCAGC CCAATGGAGC TGCCGTCAAA GAAAAGCCCA TCGGCGTGCC CAAAAATAAA 
AAAGATAAAA AGAACAAGGA CAAGGAGTAC TACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304921.1
CDS	986..5464
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEAYVGGLAHLMMGDQEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306646.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306646.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAGGCCTAT 
GTAGGAGGTC TTGCGCACCT GATGATGGGC GACCAAGAGA GAGAAGAATA TATGGCCACA 
TTCAAGGGTT CGGAGTATTT CTGTTACGAT CTGTCCCCGA ACCCCATCCA GAGCAGTAGC 
GATGAGATTA CGCTCTCCTT CAAGACCCTG CAGCGCAATG GACTGATGCT TCACACCGGA 
AAGTCTGCAG ACTACGTCAA CCTGGCGCTG AAGAACGGAG CCGTCTCGCT GGTCATCAAC 
CTGGGCTCTG GGGCCTTCGA GGCCCTGGTG GAGCCCGTCA ATGGGAAATT TAATGACAAT 
GCCTGGCATG ATGTGAAGGT TACCAGGAAC CTTCGTCAGC ACTCAGGCAT TGGACACGCT 
ATGGTTACAA TATCCGTGGA TGGGATTCTG ACCACCACTG GATATACGCA AGAGGACTAC 
ACCATGCTAG GCTCGGATGA CTTTTTCTAT GTCGGCGGTA GTCCCAGCAC TGCCGATCTT 
CCTGGATCAC CTGTCAGCAA CAACTTTATG GGCTGCTTGA AAGAGGTTGT TTACAAAAAC 
AACGATGTAA GACTTGAGCT GTCTCGACTG GCCAAGCTGG GCGACCCAAA GATGAAGGTG 
AGCGGTGTGG TGGCCTTCAA ATGTGAGAAC GTGGCTACTC TCGACCCTGT GACTTTTGAG 
ACGCCTGAGT CATTCATTAC CCTTGACAAG TGGTCCGCCA AAAAAGCCGG CTCCATCTCT 
TTCGACTTCC GCACCACTGA ACCCAACGGC CTGCTCCTCT TCAGCCATGG AAAACCCAAA 
CCCCAGCAAC AGAAAGACCC TAAGAGCCCT AAAACATTGA AGGTGGATTT CTTTGCCATT 
GAGATGCTGG ACGGCCACCT CTACCTTCTT CTCGACATGG GCTCAGGCAC CACCAAAACT 
AGAGCTGTCA ACAAGAAAGT CAATGACGGA GAATGGTACC ACGTGGACTT CCAGAGAGAT 
GGAAGATCAG GCACCATTTC CGTCAACTCG ATTCGCACTC CCTACAACGC TCCTGGTGAG 
AGTGAGATAC TGGACCTGGA CGATAAACTG TACCTGGGCG GCCTGCCAGA GGACCGGGCC 
GGTCTGATCT TCCCCACGGA GGTGTGGACC GCTCTCCTAA ATTACGGCTA TGTGGGCTGT 
GTGCGGGATC TGTTCATGGA TGGCCAGAGC AAGGACATTC GGCGGATAGC AGAGGCTCAG 
AGAGCGGTCG GGGTGAAGCC GTCTTGCTCT AAAGAACCCC CCAAACAGTG TCTGAGCAAC 
CCCTGTCTGA ACAGCGGCAC CTGCAGAGAG GGCTGGAACC GCTATGTGTG CGACTGCTCC 
GGGACGGGAT ATCTGGGACG CTCCTGTGAG AGAGATGCCA CGATCCTCAG TTACGATGGT 
AGTAAGTTCA TGAAGATCCA GCTGCCGGTG GTGATGCACA CAGAAGCGGA GGACGTGTCC 
CTGCGCTTTC GTTCTCAACG GGCCTACGGT GTTCTAATGG CGACCACATC GCAAAACTCT 
GCTGATACTC TGAGGCTGGA GCTGGACGGA GGGCGGGTCC GACTCACTGT CAATCTAGAC 
TGTATCAGGA TAAACTGTAC TACCAGTAAA GGTCCAGAGA CCATATTTTC GGGCCAGAAC 
TTGAATGACA ACGAATGGCA CACAGTTCGT GTCGTCAGAA GAGGCAAAAG TCTAAAGCTG 
ATGGTGGACG ACTTGCAGCC CTCTGAAGGT CAAATCACTG GGGACCACAC GCAGCTGGAG 
TTCCACAATG TGGAAACGGG CATCGTTACC GAGAAGCGCT ACATGCCCGC TGTGCCCTCC 
AACTTCATCG GACACCTGCA GGGTTTGTCC TTCAATGGCA TGTCCTACAT TGACTTGTGC 
AAGAACGGCG ATATCGACTA CTGCGAACTG AACGCCATGA TCGGCTACAG GAGCATCGTG 
GCAGACCCAG TGACCTTCAA GTCTCGCTCC AGCTATGTGA CTTTGCCCAC CCTGCAGGCC 
TACTATTCGA TGCACCTCTT CTTCCAGTTT AAGACTACTT CTCCAGATGG GCTCATCCTG 
TACAACCGAG GAGATGGCAA TGACTTTATA GTGGTAGAGC TGGTTAAAGG ATATCTCCAT 
TACGTGTCAG ATCTGGGAAA TGGAGCTCAT CTCATCAAAG GCAATTCCAA CACACCGCTA 
AATGACAATC ACTGGCACAA CGTGATGATC TCGCGAGATA CAAACAACCT CCATACTGTC 
AAAATAGACA CCAAAATCAC CACACAGACT ACCATGGGAG CCAAGAACCT GGATCTGAAA 
GGTGATCTGT ATGTTGGAGG TGTGGCTAAA GATATGTATA AGGACTTGCC AAAGCTGGTG 
CATTCAAAAG AGGGCTTCCA GGGCTGCCTG GCATCTGTGG ACCTGAACGG CCGCTTGCCA 
GACCTGCAGT CAGACGCACT GTCCACCGCC GGCCAGGTGG AGAGAGGCTG CGAAGGTGAG 
GAAAACGTTG CATTGCTGAA AGCTGACTTG CAAGGCCCCA GCACTACCTG TCAGGAGGAC 
TCCTGCTCCA ATCAGGGCGT GTGTCTGCAG CAGTGGGAGG GGTTTAGTTG CGACTGCAGC 
ATGACATCAT ATGGAGGGCC ACTCTGCAAT GACCCGGGAA CCACATACAT ATTCGGACGT 
GATGGAGGGC TTATTGTGTA CACATGGCCA CCCAACGACA GACCGAGCAC ACGAGCTGAC 
CGACTGGCTG TCGGTTTTAG TACCCAGCAG AAGGATGCAG TGCTGGTTAG GGTGGACAGC 
TCTTCTGGCC TGGGAGACTA CCTTCAGCTT CAGATTGAAA GAGGCAATAT CAAAGTGGTG 
TTCAATGTGG GCACCGATGA CATAAACATT GAAGAAACTT CAAAGTTTGT AAATGATGGA 
AAGTACCACA TAGTTCGCTT TACGAGAAGT GGTGGTAACG CCACCTTGCA GGTGGATGAT 
CTGCCAGTCA TTGAGCGCTA CCCATCAGGC AACATTGATA ACGAACGCCT GGCGATTGCT 
AGACAGCGAA TCCCATATCG GCTTGGTCGA GTAGTTGACG ATTGGCTACT CGACAAAGGT 
CGGCAGCTCA CCATCTTCAA CAGTCAGACC ACCATAAAGA TTGGAGGCTG GGAGAAGGGC 
AGCCGGCCCT TCCAGGGTCA GCTCTCAGGG CTTTACTACA ATGGGCTAAA AGTACTCAAC 
ATGGCCGCTG AGGGTGACCC TAATGTGCGT GTGGAGGGTA GTGCCCGGCT GGTAGGAGAC 
ATGCCCTCCT CTTCTATCAC GCCACAGTCC AGCGTCTCTG CTGCGGGAAA TCGTTCAGAA 
ACGTCACCCT CCATCACTGA CATCACCACT ACAACGGCGT CCAACCGCCA GGGCAAGCAG 
ACCACAACTC CACAGGACGA TCTTCTCGTA GCGTCTGCAG AATGCCCCAG CGATGATGAG 
GACATTGACC CTTGCGACCC AAGTTCAGGT GGGTTAGCTC ACCCACCACT TCCCGAGGCC 
AAGGGTTACC CGAGCCCAGA GGTGATTCGG GAGTCCAGCA GCACCACGGG CATGGTCGTA 
GGCATCGTTG CAGCCGCTGC ACTCTGCATC CTAATCCTGC TCTACGCCAT GTACAAATAC 
AGAAACCGCG ACGAAGGGTC TTACCACGTG GACGAGAGTC GTAACTACAT TAGTAATTCT 
GCCACGCAGC CCAATGGAGC TGCCGTCAAA GAAAAGCCCA TCGGCGTGCC CAAAAATAAA 
AAAGATAAAA AGAACAAGGA CAAGGAGTAC TACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306648.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304923.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X7, mRNA.

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CloneID	ODa44659
Clone ID Related Accession (Same CDS sequence)	XM_009306648.3 , XM_009306648.2
Accession Version	XM_009306648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGGTCTTGCG 
CACCTGATGA TGGGCGACCA AGGGAAAAGA AAAGAGAGAG AAGAATATAT GGCCACATTC 
AAGGGTTCGG AGTATTTCTG TTACGATCTG TCCCCGAACC CCATCCAGAG CAGTAGCGAT 
GAGATTACGC TCTCCTTCAA GACCCTGCAG CGCAATGGAC TGATGCTTCA CACCGGAAAG 
TCTGCAGACT ACGTCAACCT GGCGCTGAAG AACGGAGCCG TCTCGCTGGT CATCAACCTG 
GGCTCTGGGG CCTTCGAGGC CCTGGTGGAG CCCGTCAATG GGAAATTTAA TGACAATGCC 
TGGCATGATG TGAAGGTTAC CAGGAACCTT CGTCAGCACT CAGGCATTGG ACACGCTATG 
GTTACAATAT CCGTGGATGG GATTCTGACC ACCACTGGAT ATACGCAAGA GGACTACACC 
ATGCTAGGCT CGGATGACTT TTTCTATGTC GGCGGTAGTC CCAGCACTGC CGATCTTCCT 
GGATCACCTG TCAGCAACAA CTTTATGGGC TGCTTGAAAG AGGTTGTTTA CAAAAACAAC 
GATGTAAGAC TTGAGCTGTC TCGACTGGCC AAGCTGGGCG ACCCAAAGAT GAAGGTGAGC 
GGTGTGGTGG CCTTCAAATG TGAGAACGTG GCTACTCTCG ACCCTGTGAC TTTTGAGACG 
CCTGAGTCAT TCATTACCCT TGACAAGTGG TCCGCCAAAA AAGCCGGCTC CATCTCTTTC 
GACTTCCGCA CCACTGAACC CAACGGCCTG CTCCTCTTCA GCCATGGAAA ACCCAAACCC 
CAGCAACAGA AAGACCCTAA GAGCCCTAAA ACATTGAAGG TGGATTTCTT TGCCATTGAG 
ATGCTGGACG GCCACCTCTA CCTTCTTCTC GACATGGGCT CAGGCACCAC CAAAACTAGA 
GCTGTCAACA AGAAAGTCAA TGACGGAGAA TGGTACCACG TGGACTTCCA GAGAGATGGA 
AGATCAGGCA CCATTTCCGT CAACTCGATT CGCACTCCCT ACAACGCTCC TGGTGAGAGT 
GAGATACTGG ACCTGGACGA TAAACTGTAC CTGGGCGGCC TGCCAGAGGA CCGGGCCGGT 
CTGATCTTCC CCACGGAGGT GTGGACCGCT CTCCTAAATT ACGGCTATGT GGGCTGTGTG 
CGGGATCTGT TCATGGATGG CCAGAGCAAG GACATTCGGC GGATAGCAGA GGCTCAGAGA 
GCGGTCGGGG TGAAGCCGTC TTGCTCTAAA GAACCCCCCA AACAGTGTCT GAGCAACCCC 
TGTCTGAACA GCGGCACCTG CAGAGAGGGC TGGAACCGCT ATGTGTGCGA CTGCTCCGGG 
ACGGGATATC TGGGACGCTC CTGTGAGAGA GATGCCACGA TCCTCAGTTA CGATGGTAGT 
AAGTTCATGA AGATCCAGCT GCCGGTGGTG ATGCACACAG AAGCGGAGGA CGTGTCCCTG 
CGCTTTCGTT CTCAACGGGC CTACGGTGTT CTAATGGCGA CCACATCGCA AAACTCTGCT 
GATACTCTGA GGCTGGAGCT GGACGGAGGG CGGGTCCGAC TCACTGTCAA TCTAGACTGT 
ATCAGGATAA ACTGTACTAC CAGTAAAGGT CCAGAGACCA TATTTTCGGG CCAGAACTTG 
AATGACAACG AATGGCACAC AGTTCGTGTC GTCAGAAGAG GCAAAAGTCT AAAGCTGATG 
GTGGACGACT TGCAGCCCTC TGAAGGTCAA ATCACTGGGG ACCACACGCA GCTGGAGTTC 
CACAATGTGG AAACGGGCAT CGTTACCGAG AAGCGCTACA TGCCCGCTGT GCCCTCCAAC 
TTCATCGGAC ACCTGCAGGG TTTGTCCTTC AATGGCATGT CCTACATTGA CTTGTGCAAG 
AACGGCGATA TCGACTACTG CGAACTGAAC GCCATGATCG GCTACAGGAG CATCGTGGCA 
GACCCAGTGA CCTTCAAGTC TCGCTCCAGC TATGTGACTT TGCCCACCCT GCAGGCCTAC 
TATTCGATGC ACCTCTTCTT CCAGTTTAAG ACTACTTCTC CAGATGGGCT CATCCTGTAC 
AACCGAGGAG ATGGCAATGA CTTTATAGTG GTAGAGCTGG TTAAAGGATA TCTCCATTAC 
GTGTCAGATC TGGGAAATGG AGCTCATCTC ATCAAAGGCA ATTCCAACAC ACCGCTAAAT 
GACAATCACT GGCACAACGT GATGATCTCG CGAGATACAA ACAACCTCCA TACTGTCAAA 
ATAGACACCA AAATCACCAC ACAGACTACC ATGGGAGCCA AGAACCTGGA TCTGAAAGGT 
GATCTGTATG TTGGAGGTGT GGCTAAAGAT ATGTATAAGG ACTTGCCAAA GCTGGTGCAT 
TCAAAAGAGG GCTTCCAGGG CTGCCTGGCA TCTGTGGACC TGAACGGCCG CTTGCCAGAC 
CTGCAGTCAG ACGCACTGTC CACCGCCGGC CAGGTGGAGA GAGGCTGCGA AGGTGAGGAA 
AACGTTGCAT TGCTGAAAGC TGACTTGCAA GGCCCCAGCA CTACCTGTCA GGAGGACTCC 
TGCTCCAATC AGGGCGTGTG TCTGCAGCAG TGGGAGGGGT TTAGTTGCGA CTGCAGCATG 
ACATCATATG GAGGGCCACT CTGCAATGAC CCGGGAACCA CATACATATT CGGACGTGAT 
GGAGGGCTTA TTGTGTACAC ATGGCCACCC AACGACAGAC CGAGCACACG AGCTGACCGA 
CTGGCTGTCG GTTTTAGTAC CCAGCAGAAG GATGCAGTGC TGGTTAGGGT GGACAGCTCT 
TCTGGCCTGG GAGACTACCT TCAGCTTCAG ATTGAAAGAG GCAATATCAA AGTGGTGTTC 
AATGTGGGCA CCGATGACAT AAACATTGAA GAAACTTCAA AGTTTGTAAA TGATGGAAAG 
TACCACATAG TTCGCTTTAC GAGAAGTGGT GGTAACGCCA CCTTGCAGGT GGATGATCTG 
CCAGTCATTG AGCGCTACCC ATCAGGCAAC ATTGATAACG AACGCCTGGC GATTGCTAGA 
CAGCGAATCC CATATCGGCT TGGTCGAGTA GTTGACGATT GGCTACTCGA CAAAGGTCGG 
CAGCTCACCA TCTTCAACAG TCAGACCACC ATAAAGATTG GAGGCTGGGA GAAGGGCAGC 
CGGCCCTTCC AGGGTCAGCT CTCAGGGCTT TACTACAATG GGCTAAAAGT ACTCAACATG 
GCCGCTGAGG GTGACCCTAA TGTGCGTGTG GAGGGTAGTG CCCGGCTGGT AGGAGACATG 
CCCTCCTCTT CTATCACGCC ACAGTCCAGC GTCTCTGCTG CGGGAAATCG TTCAGAAACG 
TCACCCTCCA TCACTGACAT CACCACTACA ACGGCGTCCA ACCGCCAGGG CAAGCAGACC 
ACAACTCCAC AGGACGATCT TCTCGTAGCG TCTGCAGAAT GCCCCAGCGA TGATGAGGAC 
ATTGACCCTT GCGACCCAAG TTCAGGTGGG TTAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304923.1
CDS	986..5461
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306648.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306648.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGGTCTTGCG 
CACCTGATGA TGGGCGACCA AGGGAAAAGA AAAGAGAGAG AAGAATATAT GGCCACATTC 
AAGGGTTCGG AGTATTTCTG TTACGATCTG TCCCCGAACC CCATCCAGAG CAGTAGCGAT 
GAGATTACGC TCTCCTTCAA GACCCTGCAG CGCAATGGAC TGATGCTTCA CACCGGAAAG 
TCTGCAGACT ACGTCAACCT GGCGCTGAAG AACGGAGCCG TCTCGCTGGT CATCAACCTG 
GGCTCTGGGG CCTTCGAGGC CCTGGTGGAG CCCGTCAATG GGAAATTTAA TGACAATGCC 
TGGCATGATG TGAAGGTTAC CAGGAACCTT CGTCAGCACT CAGGCATTGG ACACGCTATG 
GTTACAATAT CCGTGGATGG GATTCTGACC ACCACTGGAT ATACGCAAGA GGACTACACC 
ATGCTAGGCT CGGATGACTT TTTCTATGTC GGCGGTAGTC CCAGCACTGC CGATCTTCCT 
GGATCACCTG TCAGCAACAA CTTTATGGGC TGCTTGAAAG AGGTTGTTTA CAAAAACAAC 
GATGTAAGAC TTGAGCTGTC TCGACTGGCC AAGCTGGGCG ACCCAAAGAT GAAGGTGAGC 
GGTGTGGTGG CCTTCAAATG TGAGAACGTG GCTACTCTCG ACCCTGTGAC TTTTGAGACG 
CCTGAGTCAT TCATTACCCT TGACAAGTGG TCCGCCAAAA AAGCCGGCTC CATCTCTTTC 
GACTTCCGCA CCACTGAACC CAACGGCCTG CTCCTCTTCA GCCATGGAAA ACCCAAACCC 
CAGCAACAGA AAGACCCTAA GAGCCCTAAA ACATTGAAGG TGGATTTCTT TGCCATTGAG 
ATGCTGGACG GCCACCTCTA CCTTCTTCTC GACATGGGCT CAGGCACCAC CAAAACTAGA 
GCTGTCAACA AGAAAGTCAA TGACGGAGAA TGGTACCACG TGGACTTCCA GAGAGATGGA 
AGATCAGGCA CCATTTCCGT CAACTCGATT CGCACTCCCT ACAACGCTCC TGGTGAGAGT 
GAGATACTGG ACCTGGACGA TAAACTGTAC CTGGGCGGCC TGCCAGAGGA CCGGGCCGGT 
CTGATCTTCC CCACGGAGGT GTGGACCGCT CTCCTAAATT ACGGCTATGT GGGCTGTGTG 
CGGGATCTGT TCATGGATGG CCAGAGCAAG GACATTCGGC GGATAGCAGA GGCTCAGAGA 
GCGGTCGGGG TGAAGCCGTC TTGCTCTAAA GAACCCCCCA AACAGTGTCT GAGCAACCCC 
TGTCTGAACA GCGGCACCTG CAGAGAGGGC TGGAACCGCT ATGTGTGCGA CTGCTCCGGG 
ACGGGATATC TGGGACGCTC CTGTGAGAGA GATGCCACGA TCCTCAGTTA CGATGGTAGT 
AAGTTCATGA AGATCCAGCT GCCGGTGGTG ATGCACACAG AAGCGGAGGA CGTGTCCCTG 
CGCTTTCGTT CTCAACGGGC CTACGGTGTT CTAATGGCGA CCACATCGCA AAACTCTGCT 
GATACTCTGA GGCTGGAGCT GGACGGAGGG CGGGTCCGAC TCACTGTCAA TCTAGACTGT 
ATCAGGATAA ACTGTACTAC CAGTAAAGGT CCAGAGACCA TATTTTCGGG CCAGAACTTG 
AATGACAACG AATGGCACAC AGTTCGTGTC GTCAGAAGAG GCAAAAGTCT AAAGCTGATG 
GTGGACGACT TGCAGCCCTC TGAAGGTCAA ATCACTGGGG ACCACACGCA GCTGGAGTTC 
CACAATGTGG AAACGGGCAT CGTTACCGAG AAGCGCTACA TGCCCGCTGT GCCCTCCAAC 
TTCATCGGAC ACCTGCAGGG TTTGTCCTTC AATGGCATGT CCTACATTGA CTTGTGCAAG 
AACGGCGATA TCGACTACTG CGAACTGAAC GCCATGATCG GCTACAGGAG CATCGTGGCA 
GACCCAGTGA CCTTCAAGTC TCGCTCCAGC TATGTGACTT TGCCCACCCT GCAGGCCTAC 
TATTCGATGC ACCTCTTCTT CCAGTTTAAG ACTACTTCTC CAGATGGGCT CATCCTGTAC 
AACCGAGGAG ATGGCAATGA CTTTATAGTG GTAGAGCTGG TTAAAGGATA TCTCCATTAC 
GTGTCAGATC TGGGAAATGG AGCTCATCTC ATCAAAGGCA ATTCCAACAC ACCGCTAAAT 
GACAATCACT GGCACAACGT GATGATCTCG CGAGATACAA ACAACCTCCA TACTGTCAAA 
ATAGACACCA AAATCACCAC ACAGACTACC ATGGGAGCCA AGAACCTGGA TCTGAAAGGT 
GATCTGTATG TTGGAGGTGT GGCTAAAGAT ATGTATAAGG ACTTGCCAAA GCTGGTGCAT 
TCAAAAGAGG GCTTCCAGGG CTGCCTGGCA TCTGTGGACC TGAACGGCCG CTTGCCAGAC 
CTGCAGTCAG ACGCACTGTC CACCGCCGGC CAGGTGGAGA GAGGCTGCGA AGGTGAGGAA 
AACGTTGCAT TGCTGAAAGC TGACTTGCAA GGCCCCAGCA CTACCTGTCA GGAGGACTCC 
TGCTCCAATC AGGGCGTGTG TCTGCAGCAG TGGGAGGGGT TTAGTTGCGA CTGCAGCATG 
ACATCATATG GAGGGCCACT CTGCAATGAC CCGGGAACCA CATACATATT CGGACGTGAT 
GGAGGGCTTA TTGTGTACAC ATGGCCACCC AACGACAGAC CGAGCACACG AGCTGACCGA 
CTGGCTGTCG GTTTTAGTAC CCAGCAGAAG GATGCAGTGC TGGTTAGGGT GGACAGCTCT 
TCTGGCCTGG GAGACTACCT TCAGCTTCAG ATTGAAAGAG GCAATATCAA AGTGGTGTTC 
AATGTGGGCA CCGATGACAT AAACATTGAA GAAACTTCAA AGTTTGTAAA TGATGGAAAG 
TACCACATAG TTCGCTTTAC GAGAAGTGGT GGTAACGCCA CCTTGCAGGT GGATGATCTG 
CCAGTCATTG AGCGCTACCC ATCAGGCAAC ATTGATAACG AACGCCTGGC GATTGCTAGA 
CAGCGAATCC CATATCGGCT TGGTCGAGTA GTTGACGATT GGCTACTCGA CAAAGGTCGG 
CAGCTCACCA TCTTCAACAG TCAGACCACC ATAAAGATTG GAGGCTGGGA GAAGGGCAGC 
CGGCCCTTCC AGGGTCAGCT CTCAGGGCTT TACTACAATG GGCTAAAAGT ACTCAACATG 
GCCGCTGAGG GTGACCCTAA TGTGCGTGTG GAGGGTAGTG CCCGGCTGGT AGGAGACATG 
CCCTCCTCTT CTATCACGCC ACAGTCCAGC GTCTCTGCTG CGGGAAATCG TTCAGAAACG 
TCACCCTCCA TCACTGACAT CACCACTACA ACGGCGTCCA ACCGCCAGGG CAAGCAGACC 
ACAACTCCAC AGGACGATCT TCTCGTAGCG TCTGCAGAAT GCCCCAGCGA TGATGAGGAC 
ATTGACCCTT GCGACCCAAG TTCAGGTGGG TTAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306648.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304923.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa44659
Clone ID Related Accession (Same CDS sequence)	XM_009306648.3 , XM_009306648.2
Accession Version	XM_009306648.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGGTCTTGCG 
CACCTGATGA TGGGCGACCA AGGGAAAAGA AAAGAGAGAG AAGAATATAT GGCCACATTC 
AAGGGTTCGG AGTATTTCTG TTACGATCTG TCCCCGAACC CCATCCAGAG CAGTAGCGAT 
GAGATTACGC TCTCCTTCAA GACCCTGCAG CGCAATGGAC TGATGCTTCA CACCGGAAAG 
TCTGCAGACT ACGTCAACCT GGCGCTGAAG AACGGAGCCG TCTCGCTGGT CATCAACCTG 
GGCTCTGGGG CCTTCGAGGC CCTGGTGGAG CCCGTCAATG GGAAATTTAA TGACAATGCC 
TGGCATGATG TGAAGGTTAC CAGGAACCTT CGTCAGCACT CAGGCATTGG ACACGCTATG 
GTTACAATAT CCGTGGATGG GATTCTGACC ACCACTGGAT ATACGCAAGA GGACTACACC 
ATGCTAGGCT CGGATGACTT TTTCTATGTC GGCGGTAGTC CCAGCACTGC CGATCTTCCT 
GGATCACCTG TCAGCAACAA CTTTATGGGC TGCTTGAAAG AGGTTGTTTA CAAAAACAAC 
GATGTAAGAC TTGAGCTGTC TCGACTGGCC AAGCTGGGCG ACCCAAAGAT GAAGGTGAGC 
GGTGTGGTGG CCTTCAAATG TGAGAACGTG GCTACTCTCG ACCCTGTGAC TTTTGAGACG 
CCTGAGTCAT TCATTACCCT TGACAAGTGG TCCGCCAAAA AAGCCGGCTC CATCTCTTTC 
GACTTCCGCA CCACTGAACC CAACGGCCTG CTCCTCTTCA GCCATGGAAA ACCCAAACCC 
CAGCAACAGA AAGACCCTAA GAGCCCTAAA ACATTGAAGG TGGATTTCTT TGCCATTGAG 
ATGCTGGACG GCCACCTCTA CCTTCTTCTC GACATGGGCT CAGGCACCAC CAAAACTAGA 
GCTGTCAACA AGAAAGTCAA TGACGGAGAA TGGTACCACG TGGACTTCCA GAGAGATGGA 
AGATCAGGCA CCATTTCCGT CAACTCGATT CGCACTCCCT ACAACGCTCC TGGTGAGAGT 
GAGATACTGG ACCTGGACGA TAAACTGTAC CTGGGCGGCC TGCCAGAGGA CCGGGCCGGT 
CTGATCTTCC CCACGGAGGT GTGGACCGCT CTCCTAAATT ACGGCTATGT GGGCTGTGTG 
CGGGATCTGT TCATGGATGG CCAGAGCAAG GACATTCGGC GGATAGCAGA GGCTCAGAGA 
GCGGTCGGGG TGAAGCCGTC TTGCTCTAAA GAACCCCCCA AACAGTGTCT GAGCAACCCC 
TGTCTGAACA GCGGCACCTG CAGAGAGGGC TGGAACCGCT ATGTGTGCGA CTGCTCCGGG 
ACGGGATATC TGGGACGCTC CTGTGAGAGA GATGCCACGA TCCTCAGTTA CGATGGTAGT 
AAGTTCATGA AGATCCAGCT GCCGGTGGTG ATGCACACAG AAGCGGAGGA CGTGTCCCTG 
CGCTTTCGTT CTCAACGGGC CTACGGTGTT CTAATGGCGA CCACATCGCA AAACTCTGCT 
GATACTCTGA GGCTGGAGCT GGACGGAGGG CGGGTCCGAC TCACTGTCAA TCTAGACTGT 
ATCAGGATAA ACTGTACTAC CAGTAAAGGT CCAGAGACCA TATTTTCGGG CCAGAACTTG 
AATGACAACG AATGGCACAC AGTTCGTGTC GTCAGAAGAG GCAAAAGTCT AAAGCTGATG 
GTGGACGACT TGCAGCCCTC TGAAGGTCAA ATCACTGGGG ACCACACGCA GCTGGAGTTC 
CACAATGTGG AAACGGGCAT CGTTACCGAG AAGCGCTACA TGCCCGCTGT GCCCTCCAAC 
TTCATCGGAC ACCTGCAGGG TTTGTCCTTC AATGGCATGT CCTACATTGA CTTGTGCAAG 
AACGGCGATA TCGACTACTG CGAACTGAAC GCCATGATCG GCTACAGGAG CATCGTGGCA 
GACCCAGTGA CCTTCAAGTC TCGCTCCAGC TATGTGACTT TGCCCACCCT GCAGGCCTAC 
TATTCGATGC ACCTCTTCTT CCAGTTTAAG ACTACTTCTC CAGATGGGCT CATCCTGTAC 
AACCGAGGAG ATGGCAATGA CTTTATAGTG GTAGAGCTGG TTAAAGGATA TCTCCATTAC 
GTGTCAGATC TGGGAAATGG AGCTCATCTC ATCAAAGGCA ATTCCAACAC ACCGCTAAAT 
GACAATCACT GGCACAACGT GATGATCTCG CGAGATACAA ACAACCTCCA TACTGTCAAA 
ATAGACACCA AAATCACCAC ACAGACTACC ATGGGAGCCA AGAACCTGGA TCTGAAAGGT 
GATCTGTATG TTGGAGGTGT GGCTAAAGAT ATGTATAAGG ACTTGCCAAA GCTGGTGCAT 
TCAAAAGAGG GCTTCCAGGG CTGCCTGGCA TCTGTGGACC TGAACGGCCG CTTGCCAGAC 
CTGCAGTCAG ACGCACTGTC CACCGCCGGC CAGGTGGAGA GAGGCTGCGA AGGTGAGGAA 
AACGTTGCAT TGCTGAAAGC TGACTTGCAA GGCCCCAGCA CTACCTGTCA GGAGGACTCC 
TGCTCCAATC AGGGCGTGTG TCTGCAGCAG TGGGAGGGGT TTAGTTGCGA CTGCAGCATG 
ACATCATATG GAGGGCCACT CTGCAATGAC CCGGGAACCA CATACATATT CGGACGTGAT 
GGAGGGCTTA TTGTGTACAC ATGGCCACCC AACGACAGAC CGAGCACACG AGCTGACCGA 
CTGGCTGTCG GTTTTAGTAC CCAGCAGAAG GATGCAGTGC TGGTTAGGGT GGACAGCTCT 
TCTGGCCTGG GAGACTACCT TCAGCTTCAG ATTGAAAGAG GCAATATCAA AGTGGTGTTC 
AATGTGGGCA CCGATGACAT AAACATTGAA GAAACTTCAA AGTTTGTAAA TGATGGAAAG 
TACCACATAG TTCGCTTTAC GAGAAGTGGT GGTAACGCCA CCTTGCAGGT GGATGATCTG 
CCAGTCATTG AGCGCTACCC ATCAGGCAAC ATTGATAACG AACGCCTGGC GATTGCTAGA 
CAGCGAATCC CATATCGGCT TGGTCGAGTA GTTGACGATT GGCTACTCGA CAAAGGTCGG 
CAGCTCACCA TCTTCAACAG TCAGACCACC ATAAAGATTG GAGGCTGGGA GAAGGGCAGC 
CGGCCCTTCC AGGGTCAGCT CTCAGGGCTT TACTACAATG GGCTAAAAGT ACTCAACATG 
GCCGCTGAGG GTGACCCTAA TGTGCGTGTG GAGGGTAGTG CCCGGCTGGT AGGAGACATG 
CCCTCCTCTT CTATCACGCC ACAGTCCAGC GTCTCTGCTG CGGGAAATCG TTCAGAAACG 
TCACCCTCCA TCACTGACAT CACCACTACA ACGGCGTCCA ACCGCCAGGG CAAGCAGACC 
ACAACTCCAC AGGACGATCT TCTCGTAGCG TCTGCAGAAT GCCCCAGCGA TGATGAGGAC 
ATTGACCCTT GCGACCCAAG TTCAGGTGGG TTAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304923.1
CDS	983..5458
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306648.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306648.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGGTCTTGCG 
CACCTGATGA TGGGCGACCA AGGGAAAAGA AAAGAGAGAG AAGAATATAT GGCCACATTC 
AAGGGTTCGG AGTATTTCTG TTACGATCTG TCCCCGAACC CCATCCAGAG CAGTAGCGAT 
GAGATTACGC TCTCCTTCAA GACCCTGCAG CGCAATGGAC TGATGCTTCA CACCGGAAAG 
TCTGCAGACT ACGTCAACCT GGCGCTGAAG AACGGAGCCG TCTCGCTGGT CATCAACCTG 
GGCTCTGGGG CCTTCGAGGC CCTGGTGGAG CCCGTCAATG GGAAATTTAA TGACAATGCC 
TGGCATGATG TGAAGGTTAC CAGGAACCTT CGTCAGCACT CAGGCATTGG ACACGCTATG 
GTTACAATAT CCGTGGATGG GATTCTGACC ACCACTGGAT ATACGCAAGA GGACTACACC 
ATGCTAGGCT CGGATGACTT TTTCTATGTC GGCGGTAGTC CCAGCACTGC CGATCTTCCT 
GGATCACCTG TCAGCAACAA CTTTATGGGC TGCTTGAAAG AGGTTGTTTA CAAAAACAAC 
GATGTAAGAC TTGAGCTGTC TCGACTGGCC AAGCTGGGCG ACCCAAAGAT GAAGGTGAGC 
GGTGTGGTGG CCTTCAAATG TGAGAACGTG GCTACTCTCG ACCCTGTGAC TTTTGAGACG 
CCTGAGTCAT TCATTACCCT TGACAAGTGG TCCGCCAAAA AAGCCGGCTC CATCTCTTTC 
GACTTCCGCA CCACTGAACC CAACGGCCTG CTCCTCTTCA GCCATGGAAA ACCCAAACCC 
CAGCAACAGA AAGACCCTAA GAGCCCTAAA ACATTGAAGG TGGATTTCTT TGCCATTGAG 
ATGCTGGACG GCCACCTCTA CCTTCTTCTC GACATGGGCT CAGGCACCAC CAAAACTAGA 
GCTGTCAACA AGAAAGTCAA TGACGGAGAA TGGTACCACG TGGACTTCCA GAGAGATGGA 
AGATCAGGCA CCATTTCCGT CAACTCGATT CGCACTCCCT ACAACGCTCC TGGTGAGAGT 
GAGATACTGG ACCTGGACGA TAAACTGTAC CTGGGCGGCC TGCCAGAGGA CCGGGCCGGT 
CTGATCTTCC CCACGGAGGT GTGGACCGCT CTCCTAAATT ACGGCTATGT GGGCTGTGTG 
CGGGATCTGT TCATGGATGG CCAGAGCAAG GACATTCGGC GGATAGCAGA GGCTCAGAGA 
GCGGTCGGGG TGAAGCCGTC TTGCTCTAAA GAACCCCCCA AACAGTGTCT GAGCAACCCC 
TGTCTGAACA GCGGCACCTG CAGAGAGGGC TGGAACCGCT ATGTGTGCGA CTGCTCCGGG 
ACGGGATATC TGGGACGCTC CTGTGAGAGA GATGCCACGA TCCTCAGTTA CGATGGTAGT 
AAGTTCATGA AGATCCAGCT GCCGGTGGTG ATGCACACAG AAGCGGAGGA CGTGTCCCTG 
CGCTTTCGTT CTCAACGGGC CTACGGTGTT CTAATGGCGA CCACATCGCA AAACTCTGCT 
GATACTCTGA GGCTGGAGCT GGACGGAGGG CGGGTCCGAC TCACTGTCAA TCTAGACTGT 
ATCAGGATAA ACTGTACTAC CAGTAAAGGT CCAGAGACCA TATTTTCGGG CCAGAACTTG 
AATGACAACG AATGGCACAC AGTTCGTGTC GTCAGAAGAG GCAAAAGTCT AAAGCTGATG 
GTGGACGACT TGCAGCCCTC TGAAGGTCAA ATCACTGGGG ACCACACGCA GCTGGAGTTC 
CACAATGTGG AAACGGGCAT CGTTACCGAG AAGCGCTACA TGCCCGCTGT GCCCTCCAAC 
TTCATCGGAC ACCTGCAGGG TTTGTCCTTC AATGGCATGT CCTACATTGA CTTGTGCAAG 
AACGGCGATA TCGACTACTG CGAACTGAAC GCCATGATCG GCTACAGGAG CATCGTGGCA 
GACCCAGTGA CCTTCAAGTC TCGCTCCAGC TATGTGACTT TGCCCACCCT GCAGGCCTAC 
TATTCGATGC ACCTCTTCTT CCAGTTTAAG ACTACTTCTC CAGATGGGCT CATCCTGTAC 
AACCGAGGAG ATGGCAATGA CTTTATAGTG GTAGAGCTGG TTAAAGGATA TCTCCATTAC 
GTGTCAGATC TGGGAAATGG AGCTCATCTC ATCAAAGGCA ATTCCAACAC ACCGCTAAAT 
GACAATCACT GGCACAACGT GATGATCTCG CGAGATACAA ACAACCTCCA TACTGTCAAA 
ATAGACACCA AAATCACCAC ACAGACTACC ATGGGAGCCA AGAACCTGGA TCTGAAAGGT 
GATCTGTATG TTGGAGGTGT GGCTAAAGAT ATGTATAAGG ACTTGCCAAA GCTGGTGCAT 
TCAAAAGAGG GCTTCCAGGG CTGCCTGGCA TCTGTGGACC TGAACGGCCG CTTGCCAGAC 
CTGCAGTCAG ACGCACTGTC CACCGCCGGC CAGGTGGAGA GAGGCTGCGA AGGTGAGGAA 
AACGTTGCAT TGCTGAAAGC TGACTTGCAA GGCCCCAGCA CTACCTGTCA GGAGGACTCC 
TGCTCCAATC AGGGCGTGTG TCTGCAGCAG TGGGAGGGGT TTAGTTGCGA CTGCAGCATG 
ACATCATATG GAGGGCCACT CTGCAATGAC CCGGGAACCA CATACATATT CGGACGTGAT 
GGAGGGCTTA TTGTGTACAC ATGGCCACCC AACGACAGAC CGAGCACACG AGCTGACCGA 
CTGGCTGTCG GTTTTAGTAC CCAGCAGAAG GATGCAGTGC TGGTTAGGGT GGACAGCTCT 
TCTGGCCTGG GAGACTACCT TCAGCTTCAG ATTGAAAGAG GCAATATCAA AGTGGTGTTC 
AATGTGGGCA CCGATGACAT AAACATTGAA GAAACTTCAA AGTTTGTAAA TGATGGAAAG 
TACCACATAG TTCGCTTTAC GAGAAGTGGT GGTAACGCCA CCTTGCAGGT GGATGATCTG 
CCAGTCATTG AGCGCTACCC ATCAGGCAAC ATTGATAACG AACGCCTGGC GATTGCTAGA 
CAGCGAATCC CATATCGGCT TGGTCGAGTA GTTGACGATT GGCTACTCGA CAAAGGTCGG 
CAGCTCACCA TCTTCAACAG TCAGACCACC ATAAAGATTG GAGGCTGGGA GAAGGGCAGC 
CGGCCCTTCC AGGGTCAGCT CTCAGGGCTT TACTACAATG GGCTAAAAGT ACTCAACATG 
GCCGCTGAGG GTGACCCTAA TGTGCGTGTG GAGGGTAGTG CCCGGCTGGT AGGAGACATG 
CCCTCCTCTT CTATCACGCC ACAGTCCAGC GTCTCTGCTG CGGGAAATCG TTCAGAAACG 
TCACCCTCCA TCACTGACAT CACCACTACA ACGGCGTCCA ACCGCCAGGG CAAGCAGACC 
ACAACTCCAC AGGACGATCT TCTCGTAGCG TCTGCAGAAT GCCCCAGCGA TGATGAGGAC 
ATTGACCCTT GCGACCCAAG TTCAGGTGGG TTAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306649.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304924.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X9, mRNA.

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CloneID	ODa44660
Clone ID Related Accession (Same CDS sequence)	XM_009306649.2 , XM_009306649.3
Accession Version	XM_009306649.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGGTTACA 
ATATCCGTGG ATGGGATTCT GACCACCACT GGATATACGC AAGAGGACTA CACCATGCTA 
GGCTCGGATG ACTTTTTCTA TGTCGGCGGT AGTCCCAGCA CTGCCGATCT TCCTGGATCA 
CCTGTCAGCA ACAACTTTAT GGGCTGCTTG AAAGAGGTTG TTTACAAAAA CAACGATGTA 
AGACTTGAGC TGTCTCGACT GGCCAAGCTG GGCGACCCAA AGATGAAGGT GAGCGGTGTG 
GTGGCCTTCA AATGTGAGAA CGTGGCTACT CTCGACCCTG TGACTTTTGA GACGCCTGAG 
TCATTCATTA CCCTTGACAA GTGGTCCGCC AAAAAAGCCG GCTCCATCTC TTTCGACTTC 
CGCACCACTG AACCCAACGG CCTGCTCCTC TTCAGCCATG GAAAACCCAA ACCCCAGCAA 
CAGAAAGACC CTAAGAGCCC TAAAACATTG AAGGTGGATT TCTTTGCCAT TGAGATGCTG 
GACGGCCACC TCTACCTTCT TCTCGACATG GGCTCAGGCA CCACCAAAAC TAGAGCTGTC 
AACAAGAAAG TCAATGACGG AGAATGGTAC CACGTGGACT TCCAGAGAGA TGGAAGATCA 
GGCACCATTT CCGTCAACTC GATTCGCACT CCCTACAACG CTCCTGGTGA GAGTGAGATA 
CTGGACCTGG ACGATAAACT GTACCTGGGC GGCCTGCCAG AGGACCGGGC CGGTCTGATC 
TTCCCCACGG AGGTGTGGAC CGCTCTCCTA AATTACGGCT ATGTGGGCTG TGTGCGGGAT 
CTGTTCATGG ATGGCCAGAG CAAGGACATT CGGCGGATAG CAGAGGCTCA GAGAGCGGTC 
GGGGTGAAGC CGTCTTGCTC TAAAGAACCC CCCAAACAGT GTCTGAGCAA CCCCTGTCTG 
AACAGCGGCA CCTGCAGAGA GGGCTGGAAC CGCTATGTGT GCGACTGCTC CGGGACGGGA 
TATCTGGGAC GCTCCTGTGA GAGAGATGCC ACGATCCTCA GTTACGATGG TAGTAAGTTC 
ATGAAGATCC AGCTGCCGGT GGTGATGCAC ACAGAAGCGG AGGACGTGTC CCTGCGCTTT 
CGTTCTCAAC GGGCCTACGG TGTTCTAATG GCGACCACAT CGCAAAACTC TGCTGATACT 
CTGAGGCTGG AGCTGGACGG AGGGCGGGTC CGACTCACTG TCAATCTAGA CTGTATCAGG 
ATAAACTGTA CTACCAGTAA AGGTCCAGAG ACCATATTTT CGGGCCAGAA CTTGAATGAC 
AACGAATGGC ACACAGTTCG TGTCGTCAGA AGAGGCAAAA GTCTAAAGCT GATGGTGGAC 
GACTTGCAGC CCTCTGAAGG TCAAATCACT GGGGACCACA CGCAGCTGGA GTTCCACAAT 
GTGGAAACGG GCATCGTTAC CGAGAAGCGC TACATGCCCG CTGTGCCCTC CAACTTCATC 
GGACACCTGC AGGGTTTGTC CTTCAATGGC ATGTCCTACA TTGACTTGTG CAAGAACGGC 
GATATCGACT ACTGCGAACT GAACGCCATG ATCGGCTACA GGAGCATCGT GGCAGACCCA 
GTGACCTTCA AGTCTCGCTC CAGCTATGTG ACTTTGCCCA CCCTGCAGGC CTACTATTCG 
ATGCACCTCT TCTTCCAGTT TAAGACTACT TCTCCAGATG GGCTCATCCT GTACAACCGA 
GGAGATGGCA ATGACTTTAT AGTGGTAGAG CTGGTTAAAG GATATCTCCA TTACGTGTCA 
GATCTGGGAA ATGGAGCTCA TCTCATCAAA GGCAATTCCA ACACACCGCT AAATGACAAT 
CACTGGCACA ACGTGATGAT CTCGCGAGAT ACAAACAACC TCCATACTGT CAAAATAGAC 
ACCAAAATCA CCACACAGAC TACCATGGGA GCCAAGAACC TGGATCTGAA AGGTGATCTG 
TATGTTGGAG GTGTGGCTAA AGATATGTAT AAGGACTTGC CAAAGCTGGT GCATTCAAAA 
GAGGGCTTCC AGGGCTGCCT GGCATCTGTG GACCTGAACG GCCGCTTGCC AGACCTGCAG 
TCAGACGCAC TGTCCACCGC CGGCCAGGTG GAGAGAGGCT GCGAAGGTGA GGAAAACGTT 
GCATTGCTGA AAGCTGACTT GCAAGGCCCC AGCACTACCT GTCAGGAGGA CTCCTGCTCC 
AATCAGGGCG TGTGTCTGCA GCAGTGGGAG GGGTTTAGTT GCGACTGCAG CATGACATCA 
TATGGAGGGC CACTCTGCAA TGACCCGGGA ACCACATACA TATTCGGACG TGATGGAGGG 
CTTATTGTGT ACACATGGCC ACCCAACGAC AGACCGAGCA CACGAGCTGA CCGACTGGCT 
GTCGGTTTTA GTACCCAGCA GAAGGATGCA GTGCTGGTTA GGGTGGACAG CTCTTCTGGC 
CTGGGAGACT ACCTTCAGCT TCAGATTGAA AGAGGCAATA TCAAAGTGGT GTTCAATGTG 
GGCACCGATG ACATAAACAT TGAAGAAACT TCAAAGTTTG TAAATGATGG AAAGTACCAC 
ATAGTTCGCT TTACGAGAAG TGGTGGTAAC GCCACCTTGC AGGTGGATGA TCTGCCAGTC 
ATTGAGCGCT ACCCATCAGG CAACATTGAT AACGAACGCC TGGCGATTGC TAGACAGCGA 
ATCCCATATC GGCTTGGTCG AGTAGTTGAC GATTGGCTAC TCGACAAAGG TCGGCAGCTC 
ACCATCTTCA ACAGTCAGAC CACCATAAAG ATTGGAGGCT GGGAGAAGGG CAGCCGGCCC 
TTCCAGGGTC AGCTCTCAGG GCTTTACTAC AATGGGCTAA AAGTACTCAA CATGGCCGCT 
GAGGGTGACC CTAATGTGCG TGTGGAGGGT AGTGCCCGGC TGGTAGGAGA CATGCCCTCC 
TCTTCTATCA CGCCACAGTC CAGCGTCTCT GCTGCGGGAA ATCGTTCAGA AACGTCACCC 
TCCATCACTG ACATCACCAC TACAACGGCG TCCAACCGCC AGGGCAAGCA GACCACAACT 
CCACAGGACG ATCTTCTCGT AGCGTCTGCA GAATGCCCCA GCGATGATGA GGACATTGAC 
CCTTGCGACC CAAGTTCAGG TGGGTTAGCT CACCCACCAC TTCCCGAGGC CAAGGGTTAC 
CCGAGCCCAG AGGTGATTCG GGAGTCCAGC AGCACCACGG GCATGGTCGT AGGCATCGTT 
GCAGCCGCTG CACTCTGCAT CCTAATCCTG CTCTACGCCA TGTACAAATA CAGAAACCGC 
GACGAAGGGT CTTACCACGT GGACGAGAGT CGTAACTACA TTAGTAATTC TGCCACGCAG 
CCCAATGGAG CTGCCGTCAA AGAAAAGCCC ATCGGCGTGC CCAAAAATAA AAAAGATAAA 
AAGAACAAGG ACAAGGAGTA CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304924.1
CDS	983..5452
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306649.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306649.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGGTTACA 
ATATCCGTGG ATGGGATTCT GACCACCACT GGATATACGC AAGAGGACTA CACCATGCTA 
GGCTCGGATG ACTTTTTCTA TGTCGGCGGT AGTCCCAGCA CTGCCGATCT TCCTGGATCA 
CCTGTCAGCA ACAACTTTAT GGGCTGCTTG AAAGAGGTTG TTTACAAAAA CAACGATGTA 
AGACTTGAGC TGTCTCGACT GGCCAAGCTG GGCGACCCAA AGATGAAGGT GAGCGGTGTG 
GTGGCCTTCA AATGTGAGAA CGTGGCTACT CTCGACCCTG TGACTTTTGA GACGCCTGAG 
TCATTCATTA CCCTTGACAA GTGGTCCGCC AAAAAAGCCG GCTCCATCTC TTTCGACTTC 
CGCACCACTG AACCCAACGG CCTGCTCCTC TTCAGCCATG GAAAACCCAA ACCCCAGCAA 
CAGAAAGACC CTAAGAGCCC TAAAACATTG AAGGTGGATT TCTTTGCCAT TGAGATGCTG 
GACGGCCACC TCTACCTTCT TCTCGACATG GGCTCAGGCA CCACCAAAAC TAGAGCTGTC 
AACAAGAAAG TCAATGACGG AGAATGGTAC CACGTGGACT TCCAGAGAGA TGGAAGATCA 
GGCACCATTT CCGTCAACTC GATTCGCACT CCCTACAACG CTCCTGGTGA GAGTGAGATA 
CTGGACCTGG ACGATAAACT GTACCTGGGC GGCCTGCCAG AGGACCGGGC CGGTCTGATC 
TTCCCCACGG AGGTGTGGAC CGCTCTCCTA AATTACGGCT ATGTGGGCTG TGTGCGGGAT 
CTGTTCATGG ATGGCCAGAG CAAGGACATT CGGCGGATAG CAGAGGCTCA GAGAGCGGTC 
GGGGTGAAGC CGTCTTGCTC TAAAGAACCC CCCAAACAGT GTCTGAGCAA CCCCTGTCTG 
AACAGCGGCA CCTGCAGAGA GGGCTGGAAC CGCTATGTGT GCGACTGCTC CGGGACGGGA 
TATCTGGGAC GCTCCTGTGA GAGAGATGCC ACGATCCTCA GTTACGATGG TAGTAAGTTC 
ATGAAGATCC AGCTGCCGGT GGTGATGCAC ACAGAAGCGG AGGACGTGTC CCTGCGCTTT 
CGTTCTCAAC GGGCCTACGG TGTTCTAATG GCGACCACAT CGCAAAACTC TGCTGATACT 
CTGAGGCTGG AGCTGGACGG AGGGCGGGTC CGACTCACTG TCAATCTAGA CTGTATCAGG 
ATAAACTGTA CTACCAGTAA AGGTCCAGAG ACCATATTTT CGGGCCAGAA CTTGAATGAC 
AACGAATGGC ACACAGTTCG TGTCGTCAGA AGAGGCAAAA GTCTAAAGCT GATGGTGGAC 
GACTTGCAGC CCTCTGAAGG TCAAATCACT GGGGACCACA CGCAGCTGGA GTTCCACAAT 
GTGGAAACGG GCATCGTTAC CGAGAAGCGC TACATGCCCG CTGTGCCCTC CAACTTCATC 
GGACACCTGC AGGGTTTGTC CTTCAATGGC ATGTCCTACA TTGACTTGTG CAAGAACGGC 
GATATCGACT ACTGCGAACT GAACGCCATG ATCGGCTACA GGAGCATCGT GGCAGACCCA 
GTGACCTTCA AGTCTCGCTC CAGCTATGTG ACTTTGCCCA CCCTGCAGGC CTACTATTCG 
ATGCACCTCT TCTTCCAGTT TAAGACTACT TCTCCAGATG GGCTCATCCT GTACAACCGA 
GGAGATGGCA ATGACTTTAT AGTGGTAGAG CTGGTTAAAG GATATCTCCA TTACGTGTCA 
GATCTGGGAA ATGGAGCTCA TCTCATCAAA GGCAATTCCA ACACACCGCT AAATGACAAT 
CACTGGCACA ACGTGATGAT CTCGCGAGAT ACAAACAACC TCCATACTGT CAAAATAGAC 
ACCAAAATCA CCACACAGAC TACCATGGGA GCCAAGAACC TGGATCTGAA AGGTGATCTG 
TATGTTGGAG GTGTGGCTAA AGATATGTAT AAGGACTTGC CAAAGCTGGT GCATTCAAAA 
GAGGGCTTCC AGGGCTGCCT GGCATCTGTG GACCTGAACG GCCGCTTGCC AGACCTGCAG 
TCAGACGCAC TGTCCACCGC CGGCCAGGTG GAGAGAGGCT GCGAAGGTGA GGAAAACGTT 
GCATTGCTGA AAGCTGACTT GCAAGGCCCC AGCACTACCT GTCAGGAGGA CTCCTGCTCC 
AATCAGGGCG TGTGTCTGCA GCAGTGGGAG GGGTTTAGTT GCGACTGCAG CATGACATCA 
TATGGAGGGC CACTCTGCAA TGACCCGGGA ACCACATACA TATTCGGACG TGATGGAGGG 
CTTATTGTGT ACACATGGCC ACCCAACGAC AGACCGAGCA CACGAGCTGA CCGACTGGCT 
GTCGGTTTTA GTACCCAGCA GAAGGATGCA GTGCTGGTTA GGGTGGACAG CTCTTCTGGC 
CTGGGAGACT ACCTTCAGCT TCAGATTGAA AGAGGCAATA TCAAAGTGGT GTTCAATGTG 
GGCACCGATG ACATAAACAT TGAAGAAACT TCAAAGTTTG TAAATGATGG AAAGTACCAC 
ATAGTTCGCT TTACGAGAAG TGGTGGTAAC GCCACCTTGC AGGTGGATGA TCTGCCAGTC 
ATTGAGCGCT ACCCATCAGG CAACATTGAT AACGAACGCC TGGCGATTGC TAGACAGCGA 
ATCCCATATC GGCTTGGTCG AGTAGTTGAC GATTGGCTAC TCGACAAAGG TCGGCAGCTC 
ACCATCTTCA ACAGTCAGAC CACCATAAAG ATTGGAGGCT GGGAGAAGGG CAGCCGGCCC 
TTCCAGGGTC AGCTCTCAGG GCTTTACTAC AATGGGCTAA AAGTACTCAA CATGGCCGCT 
GAGGGTGACC CTAATGTGCG TGTGGAGGGT AGTGCCCGGC TGGTAGGAGA CATGCCCTCC 
TCTTCTATCA CGCCACAGTC CAGCGTCTCT GCTGCGGGAA ATCGTTCAGA AACGTCACCC 
TCCATCACTG ACATCACCAC TACAACGGCG TCCAACCGCC AGGGCAAGCA GACCACAACT 
CCACAGGACG ATCTTCTCGT AGCGTCTGCA GAATGCCCCA GCGATGATGA GGACATTGAC 
CCTTGCGACC CAAGTTCAGG TGGGTTAGCT CACCCACCAC TTCCCGAGGC CAAGGGTTAC 
CCGAGCCCAG AGGTGATTCG GGAGTCCAGC AGCACCACGG GCATGGTCGT AGGCATCGTT 
GCAGCCGCTG CACTCTGCAT CCTAATCCTG CTCTACGCCA TGTACAAATA CAGAAACCGC 
GACGAAGGGT CTTACCACGT GGACGAGAGT CGTAACTACA TTAGTAATTC TGCCACGCAG 
CCCAATGGAG CTGCCGTCAA AGAAAAGCCC ATCGGCGTGC CCAAAAATAA AAAAGATAAA 
AAGAACAAGG ACAAGGAGTA CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306649.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304924.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X8, mRNA.

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CloneID	ODa44660
Clone ID Related Accession (Same CDS sequence)	XM_009306649.2 , XM_009306649.3
Accession Version	XM_009306649.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGGTTACA 
ATATCCGTGG ATGGGATTCT GACCACCACT GGATATACGC AAGAGGACTA CACCATGCTA 
GGCTCGGATG ACTTTTTCTA TGTCGGCGGT AGTCCCAGCA CTGCCGATCT TCCTGGATCA 
CCTGTCAGCA ACAACTTTAT GGGCTGCTTG AAAGAGGTTG TTTACAAAAA CAACGATGTA 
AGACTTGAGC TGTCTCGACT GGCCAAGCTG GGCGACCCAA AGATGAAGGT GAGCGGTGTG 
GTGGCCTTCA AATGTGAGAA CGTGGCTACT CTCGACCCTG TGACTTTTGA GACGCCTGAG 
TCATTCATTA CCCTTGACAA GTGGTCCGCC AAAAAAGCCG GCTCCATCTC TTTCGACTTC 
CGCACCACTG AACCCAACGG CCTGCTCCTC TTCAGCCATG GAAAACCCAA ACCCCAGCAA 
CAGAAAGACC CTAAGAGCCC TAAAACATTG AAGGTGGATT TCTTTGCCAT TGAGATGCTG 
GACGGCCACC TCTACCTTCT TCTCGACATG GGCTCAGGCA CCACCAAAAC TAGAGCTGTC 
AACAAGAAAG TCAATGACGG AGAATGGTAC CACGTGGACT TCCAGAGAGA TGGAAGATCA 
GGCACCATTT CCGTCAACTC GATTCGCACT CCCTACAACG CTCCTGGTGA GAGTGAGATA 
CTGGACCTGG ACGATAAACT GTACCTGGGC GGCCTGCCAG AGGACCGGGC CGGTCTGATC 
TTCCCCACGG AGGTGTGGAC CGCTCTCCTA AATTACGGCT ATGTGGGCTG TGTGCGGGAT 
CTGTTCATGG ATGGCCAGAG CAAGGACATT CGGCGGATAG CAGAGGCTCA GAGAGCGGTC 
GGGGTGAAGC CGTCTTGCTC TAAAGAACCC CCCAAACAGT GTCTGAGCAA CCCCTGTCTG 
AACAGCGGCA CCTGCAGAGA GGGCTGGAAC CGCTATGTGT GCGACTGCTC CGGGACGGGA 
TATCTGGGAC GCTCCTGTGA GAGAGATGCC ACGATCCTCA GTTACGATGG TAGTAAGTTC 
ATGAAGATCC AGCTGCCGGT GGTGATGCAC ACAGAAGCGG AGGACGTGTC CCTGCGCTTT 
CGTTCTCAAC GGGCCTACGG TGTTCTAATG GCGACCACAT CGCAAAACTC TGCTGATACT 
CTGAGGCTGG AGCTGGACGG AGGGCGGGTC CGACTCACTG TCAATCTAGA CTGTATCAGG 
ATAAACTGTA CTACCAGTAA AGGTCCAGAG ACCATATTTT CGGGCCAGAA CTTGAATGAC 
AACGAATGGC ACACAGTTCG TGTCGTCAGA AGAGGCAAAA GTCTAAAGCT GATGGTGGAC 
GACTTGCAGC CCTCTGAAGG TCAAATCACT GGGGACCACA CGCAGCTGGA GTTCCACAAT 
GTGGAAACGG GCATCGTTAC CGAGAAGCGC TACATGCCCG CTGTGCCCTC CAACTTCATC 
GGACACCTGC AGGGTTTGTC CTTCAATGGC ATGTCCTACA TTGACTTGTG CAAGAACGGC 
GATATCGACT ACTGCGAACT GAACGCCATG ATCGGCTACA GGAGCATCGT GGCAGACCCA 
GTGACCTTCA AGTCTCGCTC CAGCTATGTG ACTTTGCCCA CCCTGCAGGC CTACTATTCG 
ATGCACCTCT TCTTCCAGTT TAAGACTACT TCTCCAGATG GGCTCATCCT GTACAACCGA 
GGAGATGGCA ATGACTTTAT AGTGGTAGAG CTGGTTAAAG GATATCTCCA TTACGTGTCA 
GATCTGGGAA ATGGAGCTCA TCTCATCAAA GGCAATTCCA ACACACCGCT AAATGACAAT 
CACTGGCACA ACGTGATGAT CTCGCGAGAT ACAAACAACC TCCATACTGT CAAAATAGAC 
ACCAAAATCA CCACACAGAC TACCATGGGA GCCAAGAACC TGGATCTGAA AGGTGATCTG 
TATGTTGGAG GTGTGGCTAA AGATATGTAT AAGGACTTGC CAAAGCTGGT GCATTCAAAA 
GAGGGCTTCC AGGGCTGCCT GGCATCTGTG GACCTGAACG GCCGCTTGCC AGACCTGCAG 
TCAGACGCAC TGTCCACCGC CGGCCAGGTG GAGAGAGGCT GCGAAGGTGA GGAAAACGTT 
GCATTGCTGA AAGCTGACTT GCAAGGCCCC AGCACTACCT GTCAGGAGGA CTCCTGCTCC 
AATCAGGGCG TGTGTCTGCA GCAGTGGGAG GGGTTTAGTT GCGACTGCAG CATGACATCA 
TATGGAGGGC CACTCTGCAA TGACCCGGGA ACCACATACA TATTCGGACG TGATGGAGGG 
CTTATTGTGT ACACATGGCC ACCCAACGAC AGACCGAGCA CACGAGCTGA CCGACTGGCT 
GTCGGTTTTA GTACCCAGCA GAAGGATGCA GTGCTGGTTA GGGTGGACAG CTCTTCTGGC 
CTGGGAGACT ACCTTCAGCT TCAGATTGAA AGAGGCAATA TCAAAGTGGT GTTCAATGTG 
GGCACCGATG ACATAAACAT TGAAGAAACT TCAAAGTTTG TAAATGATGG AAAGTACCAC 
ATAGTTCGCT TTACGAGAAG TGGTGGTAAC GCCACCTTGC AGGTGGATGA TCTGCCAGTC 
ATTGAGCGCT ACCCATCAGG CAACATTGAT AACGAACGCC TGGCGATTGC TAGACAGCGA 
ATCCCATATC GGCTTGGTCG AGTAGTTGAC GATTGGCTAC TCGACAAAGG TCGGCAGCTC 
ACCATCTTCA ACAGTCAGAC CACCATAAAG ATTGGAGGCT GGGAGAAGGG CAGCCGGCCC 
TTCCAGGGTC AGCTCTCAGG GCTTTACTAC AATGGGCTAA AAGTACTCAA CATGGCCGCT 
GAGGGTGACC CTAATGTGCG TGTGGAGGGT AGTGCCCGGC TGGTAGGAGA CATGCCCTCC 
TCTTCTATCA CGCCACAGTC CAGCGTCTCT GCTGCGGGAA ATCGTTCAGA AACGTCACCC 
TCCATCACTG ACATCACCAC TACAACGGCG TCCAACCGCC AGGGCAAGCA GACCACAACT 
CCACAGGACG ATCTTCTCGT AGCGTCTGCA GAATGCCCCA GCGATGATGA GGACATTGAC 
CCTTGCGACC CAAGTTCAGG TGGGTTAGCT CACCCACCAC TTCCCGAGGC CAAGGGTTAC 
CCGAGCCCAG AGGTGATTCG GGAGTCCAGC AGCACCACGG GCATGGTCGT AGGCATCGTT 
GCAGCCGCTG CACTCTGCAT CCTAATCCTG CTCTACGCCA TGTACAAATA CAGAAACCGC 
GACGAAGGGT CTTACCACGT GGACGAGAGT CGTAACTACA TTAGTAATTC TGCCACGCAG 
CCCAATGGAG CTGCCGTCAA AGAAAAGCCC ATCGGCGTGC CCAAAAATAA AAAAGATAAA 
AAGAACAAGG ACAAGGAGTA CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304924.1
CDS	986..5455
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306649.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306649.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGGTTACA 
ATATCCGTGG ATGGGATTCT GACCACCACT GGATATACGC AAGAGGACTA CACCATGCTA 
GGCTCGGATG ACTTTTTCTA TGTCGGCGGT AGTCCCAGCA CTGCCGATCT TCCTGGATCA 
CCTGTCAGCA ACAACTTTAT GGGCTGCTTG AAAGAGGTTG TTTACAAAAA CAACGATGTA 
AGACTTGAGC TGTCTCGACT GGCCAAGCTG GGCGACCCAA AGATGAAGGT GAGCGGTGTG 
GTGGCCTTCA AATGTGAGAA CGTGGCTACT CTCGACCCTG TGACTTTTGA GACGCCTGAG 
TCATTCATTA CCCTTGACAA GTGGTCCGCC AAAAAAGCCG GCTCCATCTC TTTCGACTTC 
CGCACCACTG AACCCAACGG CCTGCTCCTC TTCAGCCATG GAAAACCCAA ACCCCAGCAA 
CAGAAAGACC CTAAGAGCCC TAAAACATTG AAGGTGGATT TCTTTGCCAT TGAGATGCTG 
GACGGCCACC TCTACCTTCT TCTCGACATG GGCTCAGGCA CCACCAAAAC TAGAGCTGTC 
AACAAGAAAG TCAATGACGG AGAATGGTAC CACGTGGACT TCCAGAGAGA TGGAAGATCA 
GGCACCATTT CCGTCAACTC GATTCGCACT CCCTACAACG CTCCTGGTGA GAGTGAGATA 
CTGGACCTGG ACGATAAACT GTACCTGGGC GGCCTGCCAG AGGACCGGGC CGGTCTGATC 
TTCCCCACGG AGGTGTGGAC CGCTCTCCTA AATTACGGCT ATGTGGGCTG TGTGCGGGAT 
CTGTTCATGG ATGGCCAGAG CAAGGACATT CGGCGGATAG CAGAGGCTCA GAGAGCGGTC 
GGGGTGAAGC CGTCTTGCTC TAAAGAACCC CCCAAACAGT GTCTGAGCAA CCCCTGTCTG 
AACAGCGGCA CCTGCAGAGA GGGCTGGAAC CGCTATGTGT GCGACTGCTC CGGGACGGGA 
TATCTGGGAC GCTCCTGTGA GAGAGATGCC ACGATCCTCA GTTACGATGG TAGTAAGTTC 
ATGAAGATCC AGCTGCCGGT GGTGATGCAC ACAGAAGCGG AGGACGTGTC CCTGCGCTTT 
CGTTCTCAAC GGGCCTACGG TGTTCTAATG GCGACCACAT CGCAAAACTC TGCTGATACT 
CTGAGGCTGG AGCTGGACGG AGGGCGGGTC CGACTCACTG TCAATCTAGA CTGTATCAGG 
ATAAACTGTA CTACCAGTAA AGGTCCAGAG ACCATATTTT CGGGCCAGAA CTTGAATGAC 
AACGAATGGC ACACAGTTCG TGTCGTCAGA AGAGGCAAAA GTCTAAAGCT GATGGTGGAC 
GACTTGCAGC CCTCTGAAGG TCAAATCACT GGGGACCACA CGCAGCTGGA GTTCCACAAT 
GTGGAAACGG GCATCGTTAC CGAGAAGCGC TACATGCCCG CTGTGCCCTC CAACTTCATC 
GGACACCTGC AGGGTTTGTC CTTCAATGGC ATGTCCTACA TTGACTTGTG CAAGAACGGC 
GATATCGACT ACTGCGAACT GAACGCCATG ATCGGCTACA GGAGCATCGT GGCAGACCCA 
GTGACCTTCA AGTCTCGCTC CAGCTATGTG ACTTTGCCCA CCCTGCAGGC CTACTATTCG 
ATGCACCTCT TCTTCCAGTT TAAGACTACT TCTCCAGATG GGCTCATCCT GTACAACCGA 
GGAGATGGCA ATGACTTTAT AGTGGTAGAG CTGGTTAAAG GATATCTCCA TTACGTGTCA 
GATCTGGGAA ATGGAGCTCA TCTCATCAAA GGCAATTCCA ACACACCGCT AAATGACAAT 
CACTGGCACA ACGTGATGAT CTCGCGAGAT ACAAACAACC TCCATACTGT CAAAATAGAC 
ACCAAAATCA CCACACAGAC TACCATGGGA GCCAAGAACC TGGATCTGAA AGGTGATCTG 
TATGTTGGAG GTGTGGCTAA AGATATGTAT AAGGACTTGC CAAAGCTGGT GCATTCAAAA 
GAGGGCTTCC AGGGCTGCCT GGCATCTGTG GACCTGAACG GCCGCTTGCC AGACCTGCAG 
TCAGACGCAC TGTCCACCGC CGGCCAGGTG GAGAGAGGCT GCGAAGGTGA GGAAAACGTT 
GCATTGCTGA AAGCTGACTT GCAAGGCCCC AGCACTACCT GTCAGGAGGA CTCCTGCTCC 
AATCAGGGCG TGTGTCTGCA GCAGTGGGAG GGGTTTAGTT GCGACTGCAG CATGACATCA 
TATGGAGGGC CACTCTGCAA TGACCCGGGA ACCACATACA TATTCGGACG TGATGGAGGG 
CTTATTGTGT ACACATGGCC ACCCAACGAC AGACCGAGCA CACGAGCTGA CCGACTGGCT 
GTCGGTTTTA GTACCCAGCA GAAGGATGCA GTGCTGGTTA GGGTGGACAG CTCTTCTGGC 
CTGGGAGACT ACCTTCAGCT TCAGATTGAA AGAGGCAATA TCAAAGTGGT GTTCAATGTG 
GGCACCGATG ACATAAACAT TGAAGAAACT TCAAAGTTTG TAAATGATGG AAAGTACCAC 
ATAGTTCGCT TTACGAGAAG TGGTGGTAAC GCCACCTTGC AGGTGGATGA TCTGCCAGTC 
ATTGAGCGCT ACCCATCAGG CAACATTGAT AACGAACGCC TGGCGATTGC TAGACAGCGA 
ATCCCATATC GGCTTGGTCG AGTAGTTGAC GATTGGCTAC TCGACAAAGG TCGGCAGCTC 
ACCATCTTCA ACAGTCAGAC CACCATAAAG ATTGGAGGCT GGGAGAAGGG CAGCCGGCCC 
TTCCAGGGTC AGCTCTCAGG GCTTTACTAC AATGGGCTAA AAGTACTCAA CATGGCCGCT 
GAGGGTGACC CTAATGTGCG TGTGGAGGGT AGTGCCCGGC TGGTAGGAGA CATGCCCTCC 
TCTTCTATCA CGCCACAGTC CAGCGTCTCT GCTGCGGGAA ATCGTTCAGA AACGTCACCC 
TCCATCACTG ACATCACCAC TACAACGGCG TCCAACCGCC AGGGCAAGCA GACCACAACT 
CCACAGGACG ATCTTCTCGT AGCGTCTGCA GAATGCCCCA GCGATGATGA GGACATTGAC 
CCTTGCGACC CAAGTTCAGG TGGGTTAGCT CACCCACCAC TTCCCGAGGC CAAGGGTTAC 
CCGAGCCCAG AGGTGATTCG GGAGTCCAGC AGCACCACGG GCATGGTCGT AGGCATCGTT 
GCAGCCGCTG CACTCTGCAT CCTAATCCTG CTCTACGCCA TGTACAAATA CAGAAACCGC 
GACGAAGGGT CTTACCACGT GGACGAGAGT CGTAACTACA TTAGTAATTC TGCCACGCAG 
CCCAATGGAG CTGCCGTCAA AGAAAAGCCC ATCGGCGTGC CCAAAAATAA AAAAGATAAA 
AAGAACAAGG ACAAGGAGTA CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_005156209.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156266.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa44661
Clone ID Related Accession (Same CDS sequence)	XM_005156209.3 , XM_005156209.4
Accession Version	XM_005156209.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156209.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGGCCCCAGC ACTACCTGTC AGGAGGACTC CTGCTCCAAT 
CAGGGCGTGT GTCTGCAGCA GTGGGAGGGG TTTAGTTGCG ACTGCAGCAT GACATCATAT 
GGAGGGCCAC TCTGCAATGA CCCGGGAACC ACATACATAT TCGGACGTGA TGGAGGGCTT 
ATTGTGTACA CATGGCCACC CAACGACAGA CCGAGCACAC GAGCTGACCG ACTGGCTGTC 
GGTTTTAGTA CCCAGCAGAA GGATGCAGTG CTGGTTAGGG TGGACAGCTC TTCTGGCCTG 
GGAGACTACC TTCAGCTTCA GATTGAAAGA GGCAATATCA AAGTGGTGTT CAATGTGGGC 
ACCGATGACA TAAACATTGA AGAAACTTCA AAGTTTGTAA ATGATGGAAA GTACCACATA 
GTTCGCTTTA CGAGAAGTGG TGGTAACGCC ACCTTGCAGG TGGATGATCT GCCAGTCATT 
GAGCGCTACC CATCAGGCAA CATTGATAAC GAACGCCTGG CGATTGCTAG ACAGCGAATC 
CCATATCGGC TTGGTCGAGT AGTTGACGAT TGGCTACTCG ACAAAGGTCG GCAGCTCACC 
ATCTTCAACA GTCAGACCAC CATAAAGATT GGAGGCTGGG AGAAGGGCAG CCGGCCCTTC 
CAGGGTCAGC TCTCAGGGCT TTACTACAAT GGGCTAAAAG TACTCAACAT GGCCGCTGAG 
GGTGACCCTA ATGTGCGTGT GGAGGGTAGT GCCCGGCTGG TAGGAGACAT GCCCTCCTCT 
TCTATCACGC CACAGTCCAG CGTCTCTGCT GCGGGAAATC GTTCAGAAAC GTCACCCTCC 
ATCACTGACA TCACCACTAC AACGGCGTCC AACCGCCAGG GCAAGCAGAC CACAACTCCA 
CAGGACGATC TTCTCGTAGC GTCTGCAGAA TGCCCCAGCG ATGATGAGGA CATTGACCCT 
TGCGACCCAA GTTCAGGTGG GTTAGCTCAC CCACCACTTC CCGAGGCCAA GGGTTACCCG 
AGCCCAGAGG TGATTCGGGA GTCCAGCAGC ACCACGGGCA TGGTCGTAGG CATCGTTGCA 
GCCGCTGCAC TCTGCATCCT AATCCTGCTC TACGCCATGT ACAAATACAG AAACCGCGAC 
GAAGGGTCTT ACCACGTGGA CGAGAGTCGT AACTACATTA GTAATTCTGC CACGCAGCCC 
AATGGAGCTG CCGTCAAAGA AAAGCCCATC GGCGTGCCCA AAAATAAAAA AGATAAAAAG 
AACAAGGACA AGGAGTACTA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156266.1
CDS	983..5449
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_005156209.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156209.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGGCCCCAGC ACTACCTGTC AGGAGGACTC CTGCTCCAAT 
CAGGGCGTGT GTCTGCAGCA GTGGGAGGGG TTTAGTTGCG ACTGCAGCAT GACATCATAT 
GGAGGGCCAC TCTGCAATGA CCCGGGAACC ACATACATAT TCGGACGTGA TGGAGGGCTT 
ATTGTGTACA CATGGCCACC CAACGACAGA CCGAGCACAC GAGCTGACCG ACTGGCTGTC 
GGTTTTAGTA CCCAGCAGAA GGATGCAGTG CTGGTTAGGG TGGACAGCTC TTCTGGCCTG 
GGAGACTACC TTCAGCTTCA GATTGAAAGA GGCAATATCA AAGTGGTGTT CAATGTGGGC 
ACCGATGACA TAAACATTGA AGAAACTTCA AAGTTTGTAA ATGATGGAAA GTACCACATA 
GTTCGCTTTA CGAGAAGTGG TGGTAACGCC ACCTTGCAGG TGGATGATCT GCCAGTCATT 
GAGCGCTACC CATCAGGCAA CATTGATAAC GAACGCCTGG CGATTGCTAG ACAGCGAATC 
CCATATCGGC TTGGTCGAGT AGTTGACGAT TGGCTACTCG ACAAAGGTCG GCAGCTCACC 
ATCTTCAACA GTCAGACCAC CATAAAGATT GGAGGCTGGG AGAAGGGCAG CCGGCCCTTC 
CAGGGTCAGC TCTCAGGGCT TTACTACAAT GGGCTAAAAG TACTCAACAT GGCCGCTGAG 
GGTGACCCTA ATGTGCGTGT GGAGGGTAGT GCCCGGCTGG TAGGAGACAT GCCCTCCTCT 
TCTATCACGC CACAGTCCAG CGTCTCTGCT GCGGGAAATC GTTCAGAAAC GTCACCCTCC 
ATCACTGACA TCACCACTAC AACGGCGTCC AACCGCCAGG GCAAGCAGAC CACAACTCCA 
CAGGACGATC TTCTCGTAGC GTCTGCAGAA TGCCCCAGCG ATGATGAGGA CATTGACCCT 
TGCGACCCAA GTTCAGGTGG GTTAGCTCAC CCACCACTTC CCGAGGCCAA GGGTTACCCG 
AGCCCAGAGG TGATTCGGGA GTCCAGCAGC ACCACGGGCA TGGTCGTAGG CATCGTTGCA 
GCCGCTGCAC TCTGCATCCT AATCCTGCTC TACGCCATGT ACAAATACAG AAACCGCGAC 
GAAGGGTCTT ACCACGTGGA CGAGAGTCGT AACTACATTA GTAATTCTGC CACGCAGCCC 
AATGGAGCTG CCGTCAAAGA AAAGCCCATC GGCGTGCCCA AAAATAAAAA AGATAAAAAG 
AACAAGGACA AGGAGTACTA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_005156209.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156266.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa44661
Clone ID Related Accession (Same CDS sequence)	XM_005156209.3 , XM_005156209.4
Accession Version	XM_005156209.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156209.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGGCCCCAGC ACTACCTGTC AGGAGGACTC CTGCTCCAAT 
CAGGGCGTGT GTCTGCAGCA GTGGGAGGGG TTTAGTTGCG ACTGCAGCAT GACATCATAT 
GGAGGGCCAC TCTGCAATGA CCCGGGAACC ACATACATAT TCGGACGTGA TGGAGGGCTT 
ATTGTGTACA CATGGCCACC CAACGACAGA CCGAGCACAC GAGCTGACCG ACTGGCTGTC 
GGTTTTAGTA CCCAGCAGAA GGATGCAGTG CTGGTTAGGG TGGACAGCTC TTCTGGCCTG 
GGAGACTACC TTCAGCTTCA GATTGAAAGA GGCAATATCA AAGTGGTGTT CAATGTGGGC 
ACCGATGACA TAAACATTGA AGAAACTTCA AAGTTTGTAA ATGATGGAAA GTACCACATA 
GTTCGCTTTA CGAGAAGTGG TGGTAACGCC ACCTTGCAGG TGGATGATCT GCCAGTCATT 
GAGCGCTACC CATCAGGCAA CATTGATAAC GAACGCCTGG CGATTGCTAG ACAGCGAATC 
CCATATCGGC TTGGTCGAGT AGTTGACGAT TGGCTACTCG ACAAAGGTCG GCAGCTCACC 
ATCTTCAACA GTCAGACCAC CATAAAGATT GGAGGCTGGG AGAAGGGCAG CCGGCCCTTC 
CAGGGTCAGC TCTCAGGGCT TTACTACAAT GGGCTAAAAG TACTCAACAT GGCCGCTGAG 
GGTGACCCTA ATGTGCGTGT GGAGGGTAGT GCCCGGCTGG TAGGAGACAT GCCCTCCTCT 
TCTATCACGC CACAGTCCAG CGTCTCTGCT GCGGGAAATC GTTCAGAAAC GTCACCCTCC 
ATCACTGACA TCACCACTAC AACGGCGTCC AACCGCCAGG GCAAGCAGAC CACAACTCCA 
CAGGACGATC TTCTCGTAGC GTCTGCAGAA TGCCCCAGCG ATGATGAGGA CATTGACCCT 
TGCGACCCAA GTTCAGGTGG GTTAGCTCAC CCACCACTTC CCGAGGCCAA GGGTTACCCG 
AGCCCAGAGG TGATTCGGGA GTCCAGCAGC ACCACGGGCA TGGTCGTAGG CATCGTTGCA 
GCCGCTGCAC TCTGCATCCT AATCCTGCTC TACGCCATGT ACAAATACAG AAACCGCGAC 
GAAGGGTCTT ACCACGTGGA CGAGAGTCGT AACTACATTA GTAATTCTGC CACGCAGCCC 
AATGGAGCTG CCGTCAAAGA AAAGCCCATC GGCGTGCCCA AAAATAAAAA AGATAAAAAG 
AACAAGGACA AGGAGTACTA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156266.1
CDS	986..5452
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_005156209.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156209.4

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGGCCCCAGC ACTACCTGTC AGGAGGACTC CTGCTCCAAT 
CAGGGCGTGT GTCTGCAGCA GTGGGAGGGG TTTAGTTGCG ACTGCAGCAT GACATCATAT 
GGAGGGCCAC TCTGCAATGA CCCGGGAACC ACATACATAT TCGGACGTGA TGGAGGGCTT 
ATTGTGTACA CATGGCCACC CAACGACAGA CCGAGCACAC GAGCTGACCG ACTGGCTGTC 
GGTTTTAGTA CCCAGCAGAA GGATGCAGTG CTGGTTAGGG TGGACAGCTC TTCTGGCCTG 
GGAGACTACC TTCAGCTTCA GATTGAAAGA GGCAATATCA AAGTGGTGTT CAATGTGGGC 
ACCGATGACA TAAACATTGA AGAAACTTCA AAGTTTGTAA ATGATGGAAA GTACCACATA 
GTTCGCTTTA CGAGAAGTGG TGGTAACGCC ACCTTGCAGG TGGATGATCT GCCAGTCATT 
GAGCGCTACC CATCAGGCAA CATTGATAAC GAACGCCTGG CGATTGCTAG ACAGCGAATC 
CCATATCGGC TTGGTCGAGT AGTTGACGAT TGGCTACTCG ACAAAGGTCG GCAGCTCACC 
ATCTTCAACA GTCAGACCAC CATAAAGATT GGAGGCTGGG AGAAGGGCAG CCGGCCCTTC 
CAGGGTCAGC TCTCAGGGCT TTACTACAAT GGGCTAAAAG TACTCAACAT GGCCGCTGAG 
GGTGACCCTA ATGTGCGTGT GGAGGGTAGT GCCCGGCTGG TAGGAGACAT GCCCTCCTCT 
TCTATCACGC CACAGTCCAG CGTCTCTGCT GCGGGAAATC GTTCAGAAAC GTCACCCTCC 
ATCACTGACA TCACCACTAC AACGGCGTCC AACCGCCAGG GCAAGCAGAC CACAACTCCA 
CAGGACGATC TTCTCGTAGC GTCTGCAGAA TGCCCCAGCG ATGATGAGGA CATTGACCCT 
TGCGACCCAA GTTCAGGTGG GTTAGCTCAC CCACCACTTC CCGAGGCCAA GGGTTACCCG 
AGCCCAGAGG TGATTCGGGA GTCCAGCAGC ACCACGGGCA TGGTCGTAGG CATCGTTGCA 
GCCGCTGCAC TCTGCATCCT AATCCTGCTC TACGCCATGT ACAAATACAG AAACCGCGAC 
GAAGGGTCTT ACCACGTGGA CGAGAGTCGT AACTACATTA GTAATTCTGC CACGCAGCCC 
AATGGAGCTG CCGTCAAAGA AAAGCCCATC GGCGTGCCCA AAAATAAAAA AGATAAAAAG 
AACAAGGACA AGGAGTACTA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306650.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304925.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X11, mRNA.

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CloneID	ODa44662
Clone ID Related Accession (Same CDS sequence)	XM_009306650.2 , XM_009306650.3
Accession Version	XM_009306650.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGTGAGGA AAACGTTGCA 
TTGCTGAAAG CTGACTTGCA AGGCCCCAGC ACTACCTGTC AGGAGGACTC CTGCTCCAAT 
CAGGGCGTGT GTCTGCAGCA GTGGGAGGGG TTTAGTTGCG ACTGCAGCAT GACATCATAT 
GGAGGGCCAC TCTGCAATGA CCCGGGAACC ACATACATAT TCGGACGTGA TGGAGGGCTT 
ATTGTGTACA CATGGCCACC CAACGACAGA CCGAGCACAC GAGCTGACCG ACTGGCTGTC 
GGTTTTAGTA CCCAGCAGAA GGATGCAGTG CTGGTTAGGG TGGACAGCTC TTCTGGCCTG 
GGAGACTACC TTCAGCTTCA GATTGAAAGA GGCAATATCA AAGTGGTGTT CAATGTGGGC 
ACCGATGACA TAAACATTGA AGAAACTTCA AAGTTTGTAA ATGATGGAAA GTACCACATA 
GTTCGCTTTA CGAGAAGTGG TGGTAACGCC ACCTTGCAGG TGGATGATCT GCCAGTCATT 
GAGCGCTACC CATCAGGCAA CATTGATAAC GAACGCCTGG CGATTGCTAG ACAGCGAATC 
CCATATCGGC TTGGTCGAGT AGTTGACGAT TGGCTACTCG ACAAAGGTCG GCAGCTCACC 
ATCTTCAACA GTCAGACCAC CATAAAGATT GGAGGCTGGG AGAAGGGCAG CCGGCCCTTC 
CAGGGTCAGC TCTCAGGGCT TTACTACAAT GGGCTAAAAG TACTCAACAT GGCCGCTGAG 
GGTGACCCTA ATGTGCGTGT GGAGGGTAGT GCCCGGCTGG TAGGAGACAT GCCCTCCTCT 
TCTATCACGC CACAGTCCAG CGTCTCTGCT GCGGGAAATC GTTCAGAAAC GTCACCCTCC 
ATCACTGACA TCACCACTAC AACGGCGTCC AACCGCCAGG GCAAGCAGAC CACAACTCCA 
CAGGACGATC TTCTCGTAGC GTCTGCAGAA TGCCCCAGCG ATGATGAGGA CATTGACCCT 
TGCGACCCAA GTTCAGGTGG GTTAGCTCAC CCACCACTTC CCGAGGCCAA GGGTTACCCG 
AGCCCAGAGG TGATTCGGGA GTCCAGCAGC ACCACGGGCA TGGTCGTAGG CATCGTTGCA 
GCCGCTGCAC TCTGCATCCT AATCCTGCTC TACGCCATGT ACAAATACAG AAACCGCGAC 
GAAGGGTCTT ACCACGTGGA CGAGAGTCGT AACTACATTA GTAATTCTGC CACGCAGCCC 
AATGGAGCTG CCGTCAAAGA AAAGCCCATC GGCGTGCCCA AAAATAAAAA AGATAAAAAG 
AACAAGGACA AGGAGTACTA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304925.1
CDS	983..5449
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLGKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306650.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306650.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGTGAGGA AAACGTTGCA 
TTGCTGAAAG CTGACTTGCA AGGCCCCAGC ACTACCTGTC AGGAGGACTC CTGCTCCAAT 
CAGGGCGTGT GTCTGCAGCA GTGGGAGGGG TTTAGTTGCG ACTGCAGCAT GACATCATAT 
GGAGGGCCAC TCTGCAATGA CCCGGGAACC ACATACATAT TCGGACGTGA TGGAGGGCTT 
ATTGTGTACA CATGGCCACC CAACGACAGA CCGAGCACAC GAGCTGACCG ACTGGCTGTC 
GGTTTTAGTA CCCAGCAGAA GGATGCAGTG CTGGTTAGGG TGGACAGCTC TTCTGGCCTG 
GGAGACTACC TTCAGCTTCA GATTGAAAGA GGCAATATCA AAGTGGTGTT CAATGTGGGC 
ACCGATGACA TAAACATTGA AGAAACTTCA AAGTTTGTAA ATGATGGAAA GTACCACATA 
GTTCGCTTTA CGAGAAGTGG TGGTAACGCC ACCTTGCAGG TGGATGATCT GCCAGTCATT 
GAGCGCTACC CATCAGGCAA CATTGATAAC GAACGCCTGG CGATTGCTAG ACAGCGAATC 
CCATATCGGC TTGGTCGAGT AGTTGACGAT TGGCTACTCG ACAAAGGTCG GCAGCTCACC 
ATCTTCAACA GTCAGACCAC CATAAAGATT GGAGGCTGGG AGAAGGGCAG CCGGCCCTTC 
CAGGGTCAGC TCTCAGGGCT TTACTACAAT GGGCTAAAAG TACTCAACAT GGCCGCTGAG 
GGTGACCCTA ATGTGCGTGT GGAGGGTAGT GCCCGGCTGG TAGGAGACAT GCCCTCCTCT 
TCTATCACGC CACAGTCCAG CGTCTCTGCT GCGGGAAATC GTTCAGAAAC GTCACCCTCC 
ATCACTGACA TCACCACTAC AACGGCGTCC AACCGCCAGG GCAAGCAGAC CACAACTCCA 
CAGGACGATC TTCTCGTAGC GTCTGCAGAA TGCCCCAGCG ATGATGAGGA CATTGACCCT 
TGCGACCCAA GTTCAGGTGG GTTAGCTCAC CCACCACTTC CCGAGGCCAA GGGTTACCCG 
AGCCCAGAGG TGATTCGGGA GTCCAGCAGC ACCACGGGCA TGGTCGTAGG CATCGTTGCA 
GCCGCTGCAC TCTGCATCCT AATCCTGCTC TACGCCATGT ACAAATACAG AAACCGCGAC 
GAAGGGTCTT ACCACGTGGA CGAGAGTCGT AACTACATTA GTAATTCTGC CACGCAGCCC 
AATGGAGCTG CCGTCAAAGA AAAGCCCATC GGCGTGCCCA AAAATAAAAA AGATAAAAAG 
AACAAGGACA AGGAGTACTA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306650.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304925.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X10, mRNA.

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CloneID	ODa44662
Clone ID Related Accession (Same CDS sequence)	XM_009306650.2 , XM_009306650.3
Accession Version	XM_009306650.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306650.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGTGAGGA AAACGTTGCA 
TTGCTGAAAG CTGACTTGCA AGGCCCCAGC ACTACCTGTC AGGAGGACTC CTGCTCCAAT 
CAGGGCGTGT GTCTGCAGCA GTGGGAGGGG TTTAGTTGCG ACTGCAGCAT GACATCATAT 
GGAGGGCCAC TCTGCAATGA CCCGGGAACC ACATACATAT TCGGACGTGA TGGAGGGCTT 
ATTGTGTACA CATGGCCACC CAACGACAGA CCGAGCACAC GAGCTGACCG ACTGGCTGTC 
GGTTTTAGTA CCCAGCAGAA GGATGCAGTG CTGGTTAGGG TGGACAGCTC TTCTGGCCTG 
GGAGACTACC TTCAGCTTCA GATTGAAAGA GGCAATATCA AAGTGGTGTT CAATGTGGGC 
ACCGATGACA TAAACATTGA AGAAACTTCA AAGTTTGTAA ATGATGGAAA GTACCACATA 
GTTCGCTTTA CGAGAAGTGG TGGTAACGCC ACCTTGCAGG TGGATGATCT GCCAGTCATT 
GAGCGCTACC CATCAGGCAA CATTGATAAC GAACGCCTGG CGATTGCTAG ACAGCGAATC 
CCATATCGGC TTGGTCGAGT AGTTGACGAT TGGCTACTCG ACAAAGGTCG GCAGCTCACC 
ATCTTCAACA GTCAGACCAC CATAAAGATT GGAGGCTGGG AGAAGGGCAG CCGGCCCTTC 
CAGGGTCAGC TCTCAGGGCT TTACTACAAT GGGCTAAAAG TACTCAACAT GGCCGCTGAG 
GGTGACCCTA ATGTGCGTGT GGAGGGTAGT GCCCGGCTGG TAGGAGACAT GCCCTCCTCT 
TCTATCACGC CACAGTCCAG CGTCTCTGCT GCGGGAAATC GTTCAGAAAC GTCACCCTCC 
ATCACTGACA TCACCACTAC AACGGCGTCC AACCGCCAGG GCAAGCAGAC CACAACTCCA 
CAGGACGATC TTCTCGTAGC GTCTGCAGAA TGCCCCAGCG ATGATGAGGA CATTGACCCT 
TGCGACCCAA GTTCAGGTGG GTTAGCTCAC CCACCACTTC CCGAGGCCAA GGGTTACCCG 
AGCCCAGAGG TGATTCGGGA GTCCAGCAGC ACCACGGGCA TGGTCGTAGG CATCGTTGCA 
GCCGCTGCAC TCTGCATCCT AATCCTGCTC TACGCCATGT ACAAATACAG AAACCGCGAC 
GAAGGGTCTT ACCACGTGGA CGAGAGTCGT AACTACATTA GTAATTCTGC CACGCAGCCC 
AATGGAGCTG CCGTCAAAGA AAAGCCCATC GGCGTGCCCA AAAATAAAAA AGATAAAAAG 
AACAAGGACA AGGAGTACTA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304925.1
CDS	986..5452
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLGKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306650.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306650.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGGTAAAGG TCCAGAGACC ATATTTTCGG GCCAGAACTT GAATGACAAC 
GAATGGCACA CAGTTCGTGT CGTCAGAAGA GGCAAAAGTC TAAAGCTGAT GGTGGACGAC 
TTGCAGCCCT CTGAAGGTCA AATCACTGGG GACCACACGC AGCTGGAGTT CCACAATGTG 
GAAACGGGCA TCGTTACCGA GAAGCGCTAC ATGCCCGCTG TGCCCTCCAA CTTCATCGGA 
CACCTGCAGG GTTTGTCCTT CAATGGCATG TCCTACATTG ACTTGTGCAA GAACGGCGAT 
ATCGACTACT GCGAACTGAA CGCCATGATC GGCTACAGGA GCATCGTGGC AGACCCAGTG 
ACCTTCAAGT CTCGCTCCAG CTATGTGACT TTGCCCACCC TGCAGGCCTA CTATTCGATG 
CACCTCTTCT TCCAGTTTAA GACTACTTCT CCAGATGGGC TCATCCTGTA CAACCGAGGA 
GATGGCAATG ACTTTATAGT GGTAGAGCTG GTTAAAGGAT ATCTCCATTA CGTGTCAGAT 
CTGGGAAATG GAGCTCATCT CATCAAAGGC AATTCCAACA CACCGCTAAA TGACAATCAC 
TGGCACAACG TGATGATCTC GCGAGATACA AACAACCTCC ATACTGTCAA AATAGACACC 
AAAATCACCA CACAGACTAC CATGGGAGCC AAGAACCTGG ATCTGAAAGG TGATCTGTAT 
GTTGGAGGTG TGGCTAAAGA TATGTATAAG GACTTGCCAA AGCTGGTGCA TTCAAAAGAG 
GGCTTCCAGG GCTGCCTGGC ATCTGTGGAC CTGAACGGCC GCTTGCCAGA CCTGCAGTCA 
GACGCACTGT CCACCGCCGG CCAGGTGGAG AGAGGCTGCG AAGGTGAGGA AAACGTTGCA 
TTGCTGAAAG CTGACTTGCA AGGCCCCAGC ACTACCTGTC AGGAGGACTC CTGCTCCAAT 
CAGGGCGTGT GTCTGCAGCA GTGGGAGGGG TTTAGTTGCG ACTGCAGCAT GACATCATAT 
GGAGGGCCAC TCTGCAATGA CCCGGGAACC ACATACATAT TCGGACGTGA TGGAGGGCTT 
ATTGTGTACA CATGGCCACC CAACGACAGA CCGAGCACAC GAGCTGACCG ACTGGCTGTC 
GGTTTTAGTA CCCAGCAGAA GGATGCAGTG CTGGTTAGGG TGGACAGCTC TTCTGGCCTG 
GGAGACTACC TTCAGCTTCA GATTGAAAGA GGCAATATCA AAGTGGTGTT CAATGTGGGC 
ACCGATGACA TAAACATTGA AGAAACTTCA AAGTTTGTAA ATGATGGAAA GTACCACATA 
GTTCGCTTTA CGAGAAGTGG TGGTAACGCC ACCTTGCAGG TGGATGATCT GCCAGTCATT 
GAGCGCTACC CATCAGGCAA CATTGATAAC GAACGCCTGG CGATTGCTAG ACAGCGAATC 
CCATATCGGC TTGGTCGAGT AGTTGACGAT TGGCTACTCG ACAAAGGTCG GCAGCTCACC 
ATCTTCAACA GTCAGACCAC CATAAAGATT GGAGGCTGGG AGAAGGGCAG CCGGCCCTTC 
CAGGGTCAGC TCTCAGGGCT TTACTACAAT GGGCTAAAAG TACTCAACAT GGCCGCTGAG 
GGTGACCCTA ATGTGCGTGT GGAGGGTAGT GCCCGGCTGG TAGGAGACAT GCCCTCCTCT 
TCTATCACGC CACAGTCCAG CGTCTCTGCT GCGGGAAATC GTTCAGAAAC GTCACCCTCC 
ATCACTGACA TCACCACTAC AACGGCGTCC AACCGCCAGG GCAAGCAGAC CACAACTCCA 
CAGGACGATC TTCTCGTAGC GTCTGCAGAA TGCCCCAGCG ATGATGAGGA CATTGACCCT 
TGCGACCCAA GTTCAGGTGG GTTAGCTCAC CCACCACTTC CCGAGGCCAA GGGTTACCCG 
AGCCCAGAGG TGATTCGGGA GTCCAGCAGC ACCACGGGCA TGGTCGTAGG CATCGTTGCA 
GCCGCTGCAC TCTGCATCCT AATCCTGCTC TACGCCATGT ACAAATACAG AAACCGCGAC 
GAAGGGTCTT ACCACGTGGA CGAGAGTCGT AACTACATTA GTAATTCTGC CACGCAGCCC 
AATGGAGCTG CCGTCAAAGA AAAGCCCATC GGCGTGCCCA AAAATAAAAA AGATAAAAAG 
AACAAGGACA AGGAGTACTA CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306651.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304926.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa44663
Clone ID Related Accession (Same CDS sequence)	XM_009306651.2 , XM_009306651.3
Accession Version	XM_009306651.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGCAACA TTGATAACGA ACGCCTGGCG ATTGCTAGAC AGCGAATCCC ATATCGGCTT 
GGTCGAGTAG TTGACGATTG GCTACTCGAC AAAGGTCGGC AGCTCACCAT CTTCAACAGT 
CAGACCACCA TAAAGATTGG AGGCTGGGAG AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC 
TCAGGGCTTT ACTACAATGG GCTAAAAGTA CTCAACATGG CCGCTGAGGG TGACCCTAAT 
GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA GGAGACATGC CCTCCTCTTC TATCACGCCA 
CAGTCCAGCG TCTCTGCTGC GGGAAATCGT TCAGAAACGT CACCCTCCAT CACTGACATC 
ACCACTACAA CGGCGTCCAA CCGCCAGGGC AAGCAGACCA CAACTCCACA GGACGATCTT 
CTCGTAGCGT CTGCAGAATG CCCCAGCGAT GATGAGGACA TTGACCCTTG CGACCCAAGT 
TCAGCTCACC CACCACTTCC CGAGGCCAAG GGTTACCCGA GCCCAGAGGT GATTCGGGAG 
TCCAGCAGCA CCACGGGCAT GGTCGTAGGC ATCGTTGCAG CCGCTGCACT CTGCATCCTA 
ATCCTGCTCT ACGCCATGTA CAAATACAGA AACCGCGACG AAGGGTCTTA CCACGTGGAC 
GAGAGTCGTA ACTACATTAG TAATTCTGCC ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA 
AAGCCCATCG GCGTGCCCAA AAATAAAAAA GATAAAAAGA ACAAGGACAA GGAGTACTAC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304926.1
CDS	983..5428
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306651.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306651.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGCAACA TTGATAACGA ACGCCTGGCG ATTGCTAGAC AGCGAATCCC ATATCGGCTT 
GGTCGAGTAG TTGACGATTG GCTACTCGAC AAAGGTCGGC AGCTCACCAT CTTCAACAGT 
CAGACCACCA TAAAGATTGG AGGCTGGGAG AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC 
TCAGGGCTTT ACTACAATGG GCTAAAAGTA CTCAACATGG CCGCTGAGGG TGACCCTAAT 
GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA GGAGACATGC CCTCCTCTTC TATCACGCCA 
CAGTCCAGCG TCTCTGCTGC GGGAAATCGT TCAGAAACGT CACCCTCCAT CACTGACATC 
ACCACTACAA CGGCGTCCAA CCGCCAGGGC AAGCAGACCA CAACTCCACA GGACGATCTT 
CTCGTAGCGT CTGCAGAATG CCCCAGCGAT GATGAGGACA TTGACCCTTG CGACCCAAGT 
TCAGCTCACC CACCACTTCC CGAGGCCAAG GGTTACCCGA GCCCAGAGGT GATTCGGGAG 
TCCAGCAGCA CCACGGGCAT GGTCGTAGGC ATCGTTGCAG CCGCTGCACT CTGCATCCTA 
ATCCTGCTCT ACGCCATGTA CAAATACAGA AACCGCGACG AAGGGTCTTA CCACGTGGAC 
GAGAGTCGTA ACTACATTAG TAATTCTGCC ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA 
AAGCCCATCG GCGTGCCCAA AAATAAAAAA GATAAAAAGA ACAAGGACAA GGAGTACTAC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306651.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304926.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ODa44663
Clone ID Related Accession (Same CDS sequence)	XM_009306651.2 , XM_009306651.3
Accession Version	XM_009306651.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306651.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGCAACA TTGATAACGA ACGCCTGGCG ATTGCTAGAC AGCGAATCCC ATATCGGCTT 
GGTCGAGTAG TTGACGATTG GCTACTCGAC AAAGGTCGGC AGCTCACCAT CTTCAACAGT 
CAGACCACCA TAAAGATTGG AGGCTGGGAG AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC 
TCAGGGCTTT ACTACAATGG GCTAAAAGTA CTCAACATGG CCGCTGAGGG TGACCCTAAT 
GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA GGAGACATGC CCTCCTCTTC TATCACGCCA 
CAGTCCAGCG TCTCTGCTGC GGGAAATCGT TCAGAAACGT CACCCTCCAT CACTGACATC 
ACCACTACAA CGGCGTCCAA CCGCCAGGGC AAGCAGACCA CAACTCCACA GGACGATCTT 
CTCGTAGCGT CTGCAGAATG CCCCAGCGAT GATGAGGACA TTGACCCTTG CGACCCAAGT 
TCAGCTCACC CACCACTTCC CGAGGCCAAG GGTTACCCGA GCCCAGAGGT GATTCGGGAG 
TCCAGCAGCA CCACGGGCAT GGTCGTAGGC ATCGTTGCAG CCGCTGCACT CTGCATCCTA 
ATCCTGCTCT ACGCCATGTA CAAATACAGA AACCGCGACG AAGGGTCTTA CCACGTGGAC 
GAGAGTCGTA ACTACATTAG TAATTCTGCC ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA 
AAGCCCATCG GCGTGCCCAA AAATAAAAAA GATAAAAAGA ACAAGGACAA GGAGTACTAC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304926.1
CDS	985..5430
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGNIDNERLAIARQRIPYRLGRVVDDWLLDKGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306651.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306651.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGCAACA TTGATAACGA ACGCCTGGCG ATTGCTAGAC AGCGAATCCC ATATCGGCTT 
GGTCGAGTAG TTGACGATTG GCTACTCGAC AAAGGTCGGC AGCTCACCAT CTTCAACAGT 
CAGACCACCA TAAAGATTGG AGGCTGGGAG AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC 
TCAGGGCTTT ACTACAATGG GCTAAAAGTA CTCAACATGG CCGCTGAGGG TGACCCTAAT 
GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA GGAGACATGC CCTCCTCTTC TATCACGCCA 
CAGTCCAGCG TCTCTGCTGC GGGAAATCGT TCAGAAACGT CACCCTCCAT CACTGACATC 
ACCACTACAA CGGCGTCCAA CCGCCAGGGC AAGCAGACCA CAACTCCACA GGACGATCTT 
CTCGTAGCGT CTGCAGAATG CCCCAGCGAT GATGAGGACA TTGACCCTTG CGACCCAAGT 
TCAGCTCACC CACCACTTCC CGAGGCCAAG GGTTACCCGA GCCCAGAGGT GATTCGGGAG 
TCCAGCAGCA CCACGGGCAT GGTCGTAGGC ATCGTTGCAG CCGCTGCACT CTGCATCCTA 
ATCCTGCTCT ACGCCATGTA CAAATACAGA AACCGCGACG AAGGGTCTTA CCACGTGGAC 
GAGAGTCGTA ACTACATTAG TAATTCTGCC ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA 
AAGCCCATCG GCGTGCCCAA AAATAAAAAA GATAAAAAGA ACAAGGACAA GGAGTACTAC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306652.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304927.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X13, mRNA.

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CloneID	ODa44664
Clone ID Related Accession (Same CDS sequence)	XM_009306652.2 , XM_009306652.3
Accession Version	XM_009306652.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGGTGGGTT AGCTCACCCA CCACTTCCCG AGGCCAAGGG TTACCCGAGC 
CCAGAGGTGA TTCGGGAGTC CAGCAGCACC ACGGGCATGG TCGTAGGCAT CGTTGCAGCC 
GCTGCACTCT GCATCCTAAT CCTGCTCTAC GCCATGTACA AATACAGAAA CCGCGACGAA 
GGGTCTTACC ACGTGGACGA GAGTCGTAAC TACATTAGTA ATTCTGCCAC GCAGCCCAAT 
GGAGCTGCCG TCAAAGAAAA GCCCATCGGC GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC 
AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304927.1
CDS	983..5386
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306652.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306652.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGGTGGGTT AGCTCACCCA CCACTTCCCG AGGCCAAGGG TTACCCGAGC 
CCAGAGGTGA TTCGGGAGTC CAGCAGCACC ACGGGCATGG TCGTAGGCAT CGTTGCAGCC 
GCTGCACTCT GCATCCTAAT CCTGCTCTAC GCCATGTACA AATACAGAAA CCGCGACGAA 
GGGTCTTACC ACGTGGACGA GAGTCGTAAC TACATTAGTA ATTCTGCCAC GCAGCCCAAT 
GGAGCTGCCG TCAAAGAAAA GCCCATCGGC GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC 
AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306652.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304927.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X17, mRNA.

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CloneID	ODa44664
Clone ID Related Accession (Same CDS sequence)	XM_009306652.2 , XM_009306652.3
Accession Version	XM_009306652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGGTGGGTT AGCTCACCCA CCACTTCCCG AGGCCAAGGG TTACCCGAGC 
CCAGAGGTGA TTCGGGAGTC CAGCAGCACC ACGGGCATGG TCGTAGGCAT CGTTGCAGCC 
GCTGCACTCT GCATCCTAAT CCTGCTCTAC GCCATGTACA AATACAGAAA CCGCGACGAA 
GGGTCTTACC ACGTGGACGA GAGTCGTAAC TACATTAGTA ATTCTGCCAC GCAGCCCAAT 
GGAGCTGCCG TCAAAGAAAA GCCCATCGGC GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC 
AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304927.1
CDS	986..5389
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306652.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306652.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGGTGGGTT AGCTCACCCA CCACTTCCCG AGGCCAAGGG TTACCCGAGC 
CCAGAGGTGA TTCGGGAGTC CAGCAGCACC ACGGGCATGG TCGTAGGCAT CGTTGCAGCC 
GCTGCACTCT GCATCCTAAT CCTGCTCTAC GCCATGTACA AATACAGAAA CCGCGACGAA 
GGGTCTTACC ACGTGGACGA GAGTCGTAAC TACATTAGTA ATTCTGCCAC GCAGCCCAAT 
GGAGCTGCCG TCAAAGAAAA GCCCATCGGC GTGCCCAAAA ATAAAAAAGA TAAAAAGAAC 
AAGGACAAGG AGTACTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306653.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304928.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X18, mRNA.

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CloneID	ODa44665
Clone ID Related Accession (Same CDS sequence)	XM_009306653.3 , XM_009306653.2
Accession Version	XM_009306653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGCTCACCC ACCACTTCCC GAGGCCAAGG GTTACCCGAG CCCAGAGGTG 
ATTCGGGAGT CCAGCAGCAC CACGGGCATG GTCGTAGGCA TCGTTGCAGC CGCTGCACTC 
TGCATCCTAA TCCTGCTCTA CGCCATGTAC AAATACAGAA ACCGCGACGA AGGGTCTTAC 
CACGTGGACG AGAGTCGTAA CTACATTAGT AATTCTGCCA CGCAGCCCAA TGGAGCTGCC 
GTCAAAGAAA AGCCCATCGG CGTGCCCAAA AATAAAAAAG ATAAAAAGAA CAAGGACAAG 
GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304928.1
CDS	986..5380
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306653.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306653.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGCTCACCC ACCACTTCCC GAGGCCAAGG GTTACCCGAG CCCAGAGGTG 
ATTCGGGAGT CCAGCAGCAC CACGGGCATG GTCGTAGGCA TCGTTGCAGC CGCTGCACTC 
TGCATCCTAA TCCTGCTCTA CGCCATGTAC AAATACAGAA ACCGCGACGA AGGGTCTTAC 
CACGTGGACG AGAGTCGTAA CTACATTAGT AATTCTGCCA CGCAGCCCAA TGGAGCTGCC 
GTCAAAGAAA AGCCCATCGG CGTGCCCAAA AATAAAAAAG ATAAAAAGAA CAAGGACAAG 
GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306653.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304928.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa44665
Clone ID Related Accession (Same CDS sequence)	XM_009306653.3 , XM_009306653.2
Accession Version	XM_009306653.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGCTCACCC ACCACTTCCC GAGGCCAAGG GTTACCCGAG CCCAGAGGTG 
ATTCGGGAGT CCAGCAGCAC CACGGGCATG GTCGTAGGCA TCGTTGCAGC CGCTGCACTC 
TGCATCCTAA TCCTGCTCTA CGCCATGTAC AAATACAGAA ACCGCGACGA AGGGTCTTAC 
CACGTGGACG AGAGTCGTAA CTACATTAGT AATTCTGCCA CGCAGCCCAA TGGAGCTGCC 
GTCAAAGAAA AGCCCATCGG CGTGCCCAAA AATAAAAAAG ATAAAAAGAA CAAGGACAAG 
GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304928.1
CDS	983..5377
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGEENVALLKADLQGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306653.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306653.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GTGAGGAAAA CGTTGCATTG CTGAAAGCTG ACTTGCAAGG CCCCAGCACT 
ACCTGTCAGG AGGACTCCTG CTCCAATCAG GGCGTGTGTC TGCAGCAGTG GGAGGGGTTT 
AGTTGCGACT GCAGCATGAC ATCATATGGA GGGCCACTCT GCAATGACCC GGGAACCACA 
TACATATTCG GACGTGATGG AGGGCTTATT GTGTACACAT GGCCACCCAA CGACAGACCG 
AGCACACGAG CTGACCGACT GGCTGTCGGT TTTAGTACCC AGCAGAAGGA TGCAGTGCTG 
GTTAGGGTGG ACAGCTCTTC TGGCCTGGGA GACTACCTTC AGCTTCAGAT TGAAAGAGGC 
AATATCAAAG TGGTGTTCAA TGTGGGCACC GATGACATAA ACATTGAAGA AACTTCAAAG 
TTTGTAAATG ATGGAAAGTA CCACATAGTT CGCTTTACGA GAAGTGGTGG TAACGCCACC 
TTGCAGGTGG ATGATCTGCC AGTCATTGAG CGCTACCCAT CAGGTCGGCA GCTCACCATC 
TTCAACAGTC AGACCACCAT AAAGATTGGA GGCTGGGAGA AGGGCAGCCG GCCCTTCCAG 
GGTCAGCTCT CAGGGCTTTA CTACAATGGG CTAAAAGTAC TCAACATGGC CGCTGAGGGT 
GACCCTAATG TGCGTGTGGA GGGTAGTGCC CGGCTGGTAG GAGACATGCC CTCCTCTTCT 
ATCACGCCAC AGTCCAGCGT CTCTGCTGCG GGAAATCGTT CAGAAACGTC ACCCTCCATC 
ACTGACATCA CCACTACAAC GGCGTCCAAC CGCCAGGGCA AGCAGACCAC AACTCCACAG 
GACGATCTTC TCGTAGCGTC TGCAGAATGC CCCAGCGATG ATGAGGACAT TGACCCTTGC 
GACCCAAGTT CAGCTCACCC ACCACTTCCC GAGGCCAAGG GTTACCCGAG CCCAGAGGTG 
ATTCGGGAGT CCAGCAGCAC CACGGGCATG GTCGTAGGCA TCGTTGCAGC CGCTGCACTC 
TGCATCCTAA TCCTGCTCTA CGCCATGTAC AAATACAGAA ACCGCGACGA AGGGTCTTAC 
CACGTGGACG AGAGTCGTAA CTACATTAGT AATTCTGCCA CGCAGCCCAA TGGAGCTGCC 
GTCAAAGAAA AGCCCATCGG CGTGCCCAAA AATAAAAAAG ATAAAAAGAA CAAGGACAAG 
GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306654.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304929.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X15, mRNA.

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CloneID	ODa44666
Clone ID Related Accession (Same CDS sequence)	XM_009306654.2 , XM_009306654.3
Accession Version	XM_009306654.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGGTGGGT TAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304929.1
CDS	983..5347
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306654.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306654.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGGTGGGT TAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306654.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304929.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X19, mRNA.

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CloneID	ODa44666
Clone ID Related Accession (Same CDS sequence)	XM_009306654.2 , XM_009306654.3
Accession Version	XM_009306654.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306654.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGGTGGGT TAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304929.1
CDS	986..5350
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSGGLAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306654.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306654.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGGTGGGT TAGCTCACCC ACCACTTCCC 
GAGGCCAAGG GTTACCCGAG CCCAGAGGTG ATTCGGGAGT CCAGCAGCAC CACGGGCATG 
GTCGTAGGCA TCGTTGCAGC CGCTGCACTC TGCATCCTAA TCCTGCTCTA CGCCATGTAC 
AAATACAGAA ACCGCGACGA AGGGTCTTAC CACGTGGACG AGAGTCGTAA CTACATTAGT 
AATTCTGCCA CGCAGCCCAA TGGAGCTGCC GTCAAAGAAA AGCCCATCGG CGTGCCCAAA 
AATAAAAAAG ATAAAAAGAA CAAGGACAAG GAGTACTACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306656.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304931.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ODa44668
Clone ID Related Accession (Same CDS sequence)	XM_009306656.2 , XM_009306656.3
Accession Version	XM_009306656.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurexin 1a isoform X19
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304931.1
CDS	983..5338
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306656.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306656.2

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrxn1a ( XM_009306656.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304931.1 Danio rerio neurexin 1a (nrxn1a), transcript variant X26, mRNA.

Price & Availability↑

CloneID	ODa44668
Clone ID Related Accession (Same CDS sequence)	XM_009306656.2 , XM_009306656.3
Accession Version	XM_009306656.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurexin 1a isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304931.1
CDS	986..5341
Translation	MSFSMRNGAHLIWIGLLVCCLVDMGASMEFTGAEGQWARFPMWNACCESEMSFNMKTKSAHGLLVYFDDEGFCDFLELLIHNGRLSLRFSIFCAEPATVFSDTAVNDSRWHAVTLRRNFKNTTLVVDEEIKWVEVKSKRRDMTVFSHLFLGGIPPELRSVALRLTSSAIKDEVPYKGWITNLRVNGSEPVLIGSDGVNSDICEADHICLNGGVCSIVNDEPICDCSETGFQGKDCSEEEAYVGGLAHLMMGDQGKRKEREEYMATFKGSEYFCYDLSPNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKLGDPKMKVSGVVAFKCENVATLDPVTFETPESFITLDKWSAKKAGSISFDFRTTEPNGLLLFSHGKPKPQQQKDPKSPKTLKVDFFAIEMLDGHLYLLLDMGSGTTKTRAVNKKVNDGEWYHVDFQRDGRSGTISVNSIRTPYNAPGESEILDLDDKLYLGGLPEDRAGLIFPTEVWTALLNYGYVGCVRDLFMDGQSKDIRRIAEAQRAVGVKPSCSKEPPKQCLSNPCLNSGTCREGWNRYVCDCSGTGYLGRSCERDATILSYDGSKFMKIQLPVVMHTEAEDVSLRFRSQRAYGVLMATTSQNSADTLRLELDGGRVRLTVNLDCIRINCTTSKGPETIFSGQNLNDNEWHTVRVVRRGKSLKLMVDDLQPSEGQITGDHTQLEFHNVETGIVTEKRYMPAVPSNFIGHLQGLSFNGMSYIDLCKNGDIDYCELNAMIGYRSIVADPVTFKSRSSYVTLPTLQAYYSMHLFFQFKTTSPDGLILYNRGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNTPLNDNHWHNVMISRDTNNLHTVKIDTKITTQTTMGAKNLDLKGDLYVGGVAKDMYKDLPKLVHSKEGFQGCLASVDLNGRLPDLQSDALSTAGQVERGCEGPSTTCQEDSCSNQGVCLQQWEGFSCDCSMTSYGGPLCNDPGTTYIFGRDGGLIVYTWPPNDRPSTRADRLAVGFSTQQKDAVLVRVDSSSGLGDYLQLQIERGNIKVVFNVGTDDINIEETSKFVNDGKYHIVRFTRSGGNATLQVDDLPVIERYPSGRQLTIFNSQTTIKIGGWEKGSRPFQGQLSGLYYNGLKVLNMAAEGDPNVRVEGSARLVGDMPSSSITPQSSVSAAGNRSETSPSITDITTTTASNRQGKQTTTPQDDLLVASAECPSDDEDIDPCDPSSAHPPLPEAKGYPSPEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSATQPNGAAVKEKPIGVPKNKKDKKNKDKEYYV

Target ORF information:

RefSeq Version	XM_009306656.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurexin 1a (nrxn1a), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306656.3

ORF Insert Sequence:

ATGAGCTTCT CAATGCGGAA CGGTGCACAT CTAATCTGGA TAGGCCTACT GGTTTGTTGC 
CTTGTCGACA TGGGGGCCAG CATGGAGTTC ACTGGTGCAG AAGGCCAGTG GGCAAGGTTT 
CCCATGTGGA ACGCCTGCTG CGAAAGCGAA ATGAGTTTCA ACATGAAGAC CAAAAGCGCT 
CATGGACTTT TAGTGTACTT TGACGACGAG GGATTCTGTG ACTTCTTGGA ACTGCTCATC 
CACAATGGTA GACTCAGTTT GCGGTTCTCC ATCTTCTGCG CCGAGCCAGC GACCGTGTTC 
TCAGACACAG CCGTCAATGA CAGCCGCTGG CACGCCGTGA CCTTGCGCAG GAATTTCAAA 
AACACTACTT TAGTTGTGGA CGAAGAAATC AAGTGGGTGG AGGTAAAGTC GAAACGGAGG 
GACATGACGG TCTTCAGCCA TTTATTCTTA GGGGGGATAC CTCCTGAACT GCGATCTGTA 
GCATTACGCC TCACATCTTC AGCCATAAAA GATGAGGTTC CCTACAAAGG ATGGATAACC 
AACCTGAGAG TGAACGGCTC GGAGCCGGTG CTTATCGGTA GCGATGGAGT CAACAGCGAC 
ATTTGCGAAG CCGACCACAT TTGCCTGAAT GGAGGAGTCT GCAGCATTGT CAACGATGAG 
CCCATTTGCG ACTGCTCTGA AACGGGATTC CAGGGAAAAG ACTGCAGTGA AGAAGAGGCC 
TATGTAGGAG GTCTTGCGCA CCTGATGATG GGCGACCAAG GGAAAAGAAA AGAGAGAGAA 
GAATATATGG CCACATTCAA GGGTTCGGAG TATTTCTGTT ACGATCTGTC CCCGAACCCC 
ATCCAGAGCA GTAGCGATGA GATTACGCTC TCCTTCAAGA CCCTGCAGCG CAATGGACTG 
ATGCTTCACA CCGGAAAGTC TGCAGACTAC GTCAACCTGG CGCTGAAGAA CGGAGCCGTC 
TCGCTGGTCA TCAACCTGGG CTCTGGGGCC TTCGAGGCCC TGGTGGAGCC CGTCAATGGG 
AAATTTAATG ACAATGCCTG GCATGATGTG AAGGTTACCA GGAACCTTCG TCAGCACTCA 
GGCATTGGAC ACGCTATGGT TACAATATCC GTGGATGGGA TTCTGACCAC CACTGGATAT 
ACGCAAGAGG ACTACACCAT GCTAGGCTCG GATGACTTTT TCTATGTCGG CGGTAGTCCC 
AGCACTGCCG ATCTTCCTGG ATCACCTGTC AGCAACAACT TTATGGGCTG CTTGAAAGAG 
GTTGTTTACA AAAACAACGA TGTAAGACTT GAGCTGTCTC GACTGGCCAA GCTGGGCGAC 
CCAAAGATGA AGGTGAGCGG TGTGGTGGCC TTCAAATGTG AGAACGTGGC TACTCTCGAC 
CCTGTGACTT TTGAGACGCC TGAGTCATTC ATTACCCTTG ACAAGTGGTC CGCCAAAAAA 
GCCGGCTCCA TCTCTTTCGA CTTCCGCACC ACTGAACCCA ACGGCCTGCT CCTCTTCAGC 
CATGGAAAAC CCAAACCCCA GCAACAGAAA GACCCTAAGA GCCCTAAAAC ATTGAAGGTG 
GATTTCTTTG CCATTGAGAT GCTGGACGGC CACCTCTACC TTCTTCTCGA CATGGGCTCA 
GGCACCACCA AAACTAGAGC TGTCAACAAG AAAGTCAATG ACGGAGAATG GTACCACGTG 
GACTTCCAGA GAGATGGAAG ATCAGGCACC ATTTCCGTCA ACTCGATTCG CACTCCCTAC 
AACGCTCCTG GTGAGAGTGA GATACTGGAC CTGGACGATA AACTGTACCT GGGCGGCCTG 
CCAGAGGACC GGGCCGGTCT GATCTTCCCC ACGGAGGTGT GGACCGCTCT CCTAAATTAC 
GGCTATGTGG GCTGTGTGCG GGATCTGTTC ATGGATGGCC AGAGCAAGGA CATTCGGCGG 
ATAGCAGAGG CTCAGAGAGC GGTCGGGGTG AAGCCGTCTT GCTCTAAAGA ACCCCCCAAA 
CAGTGTCTGA GCAACCCCTG TCTGAACAGC GGCACCTGCA GAGAGGGCTG GAACCGCTAT 
GTGTGCGACT GCTCCGGGAC GGGATATCTG GGACGCTCCT GTGAGAGAGA TGCCACGATC 
CTCAGTTACG ATGGTAGTAA GTTCATGAAG ATCCAGCTGC CGGTGGTGAT GCACACAGAA 
GCGGAGGACG TGTCCCTGCG CTTTCGTTCT CAACGGGCCT ACGGTGTTCT AATGGCGACC 
ACATCGCAAA ACTCTGCTGA TACTCTGAGG CTGGAGCTGG ACGGAGGGCG GGTCCGACTC 
ACTGTCAATC TAGACTGTAT CAGGATAAAC TGTACTACCA GTAAAGGTCC AGAGACCATA 
TTTTCGGGCC AGAACTTGAA TGACAACGAA TGGCACACAG TTCGTGTCGT CAGAAGAGGC 
AAAAGTCTAA AGCTGATGGT GGACGACTTG CAGCCCTCTG AAGGTCAAAT CACTGGGGAC 
CACACGCAGC TGGAGTTCCA CAATGTGGAA ACGGGCATCG TTACCGAGAA GCGCTACATG 
CCCGCTGTGC CCTCCAACTT CATCGGACAC CTGCAGGGTT TGTCCTTCAA TGGCATGTCC 
TACATTGACT TGTGCAAGAA CGGCGATATC GACTACTGCG AACTGAACGC CATGATCGGC 
TACAGGAGCA TCGTGGCAGA CCCAGTGACC TTCAAGTCTC GCTCCAGCTA TGTGACTTTG 
CCCACCCTGC AGGCCTACTA TTCGATGCAC CTCTTCTTCC AGTTTAAGAC TACTTCTCCA 
GATGGGCTCA TCCTGTACAA CCGAGGAGAT GGCAATGACT TTATAGTGGT AGAGCTGGTT 
AAAGGATATC TCCATTACGT GTCAGATCTG GGAAATGGAG CTCATCTCAT CAAAGGCAAT 
TCCAACACAC CGCTAAATGA CAATCACTGG CACAACGTGA TGATCTCGCG AGATACAAAC 
AACCTCCATA CTGTCAAAAT AGACACCAAA ATCACCACAC AGACTACCAT GGGAGCCAAG 
AACCTGGATC TGAAAGGTGA TCTGTATGTT GGAGGTGTGG CTAAAGATAT GTATAAGGAC 
TTGCCAAAGC TGGTGCATTC AAAAGAGGGC TTCCAGGGCT GCCTGGCATC TGTGGACCTG 
AACGGCCGCT TGCCAGACCT GCAGTCAGAC GCACTGTCCA CCGCCGGCCA GGTGGAGAGA 
GGCTGCGAAG GCCCCAGCAC TACCTGTCAG GAGGACTCCT GCTCCAATCA GGGCGTGTGT 
CTGCAGCAGT GGGAGGGGTT TAGTTGCGAC TGCAGCATGA CATCATATGG AGGGCCACTC 
TGCAATGACC CGGGAACCAC ATACATATTC GGACGTGATG GAGGGCTTAT TGTGTACACA 
TGGCCACCCA ACGACAGACC GAGCACACGA GCTGACCGAC TGGCTGTCGG TTTTAGTACC 
CAGCAGAAGG ATGCAGTGCT GGTTAGGGTG GACAGCTCTT CTGGCCTGGG AGACTACCTT 
CAGCTTCAGA TTGAAAGAGG CAATATCAAA GTGGTGTTCA ATGTGGGCAC CGATGACATA 
AACATTGAAG AAACTTCAAA GTTTGTAAAT GATGGAAAGT ACCACATAGT TCGCTTTACG 
AGAAGTGGTG GTAACGCCAC CTTGCAGGTG GATGATCTGC CAGTCATTGA GCGCTACCCA 
TCAGGTCGGC AGCTCACCAT CTTCAACAGT CAGACCACCA TAAAGATTGG AGGCTGGGAG 
AAGGGCAGCC GGCCCTTCCA GGGTCAGCTC TCAGGGCTTT ACTACAATGG GCTAAAAGTA 
CTCAACATGG CCGCTGAGGG TGACCCTAAT GTGCGTGTGG AGGGTAGTGC CCGGCTGGTA 
GGAGACATGC CCTCCTCTTC TATCACGCCA CAGTCCAGCG TCTCTGCTGC GGGAAATCGT 
TCAGAAACGT CACCCTCCAT CACTGACATC ACCACTACAA CGGCGTCCAA CCGCCAGGGC 
AAGCAGACCA CAACTCCACA GGACGATCTT CTCGTAGCGT CTGCAGAATG CCCCAGCGAT 
GATGAGGACA TTGACCCTTG CGACCCAAGT TCAGCTCACC CACCACTTCC CGAGGCCAAG 
GGTTACCCGA GCCCAGAGGT GATTCGGGAG TCCAGCAGCA CCACGGGCAT GGTCGTAGGC 
ATCGTTGCAG CCGCTGCACT CTGCATCCTA ATCCTGCTCT ACGCCATGTA CAAATACAGA 
AACCGCGACG AAGGGTCTTA CCACGTGGAC GAGAGTCGTA ACTACATTAG TAATTCTGCC 
ACGCAGCCCA ATGGAGCTGC CGTCAAAGAA AAGCCCATCG GCGTGCCCAA AAATAAAAAA 
GATAAAAAGA ACAAGGACAA GGAGTACTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of neurexin2a in lead-induced locomotor defect in developing zebrafish.
Aquatic toxicology (Amsterdam, Netherlands)194167-175(2018 Jan)
Tu H,Peng T,Liu J,Chen X,Fan C,Huang Z,Zhang Y,Zou F,Meng X

Maternal and embryonic exposure to the water soluble fraction of crude oil or lead induces behavioral abnormalities in zebrafish (Danio rerio), and the mechanisms involved.
Chemosphere1917-16(2018 Jan)
Wang Y,Shen C,Wang C,Zhou Y,Gao D,Zuo Z

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Demonstration of the Coexistence of Duplicated LH Receptors in Teleosts, and Their Origin in Ancestral Actinopterygians.
PloS one10(8)e0135184(2015)
Maugars G,Dufour S

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nrxn1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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