TJP2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TJP2A
Entrez Gene ID	565446
Full Name	tight junction protein 2a (zona occludens 2)
Synonyms	tjp2.1,wu:fb10a11,zo-2.1
General protein information	Preferred Names tight junction protein 2a (zona occludens 2) Names tight junction protein ZO-2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155574.4	XP_005155631.1	tight junction protein ZO-2 isoform X2
NM_001201570.1	NP_001188499.1	tight junction protein ZO-2 isoform 1
XM_009305192.2	XP_009303467.1	tight junction protein ZO-2 isoform X1
XM_009305192.3	XP_009303467.1	tight junction protein ZO-2 isoform X1
NM_001201571.1	NP_001188500.1	tight junction protein ZO-2 isoform 2
XM_005155574.3	XP_005155631.1	tight junction protein ZO-2 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TJP2	XP_003312175.1
Danio rerio (zebrafish)	tjp2a	NP_001188499.1
Xenopus tropicalis (tropical clawed frog)	tjp2	XP_004910833.1
	LOC699586	XP_002808149.1
Canis lupus familiaris (dog)	TJP2	NP_001003204.1
Mus musculus (house mouse)	Tjp2	NP_001185914.1
Gallus gallus (chicken)	TJP2	NP_990249.1
Homo sapiens (human)	TJP2	NP_001163887.1
Rattus norvegicus (Norway rat)	Tjp2	NP_446225.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth.
Mayrhofer M, Gourain V, Reischl M, Affaticati P, Jenett A, Joly JS, Benelli M, Demichelis F, Poliani PL, Sieger D, Mione M
Disease models & mechanisms10(1)15-28(2017 01)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TJP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00390	NM_001201570.1 Latest version!	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa35091	NM_001201571.1 Latest version!	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa15901	XM_005155574.4 Latest version!	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa44613	XM_009305192.3 Latest version!	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa44613	XM_009305192.2	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa15901	XM_005155574.3	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TJP2A ( NM_001201570.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001188499.1 Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa00390
Clone ID Related Accession (Same CDS sequence)	NM_001201570.1
Accession Version	NM_001201570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX640481.14 and BX629350.6. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGTCG TTGTGGAGAA ACGGCAGTTT CTGTGTAGAA ATGTTAAGGA CTGTTATTAT 
AACCCAGTCA TGGAGGAGAC TGTGTGGGAG CAGTACACTG TGACCCTACA GCGGGACTCC 
AAGATGGGCT TTGGCCTAGC GGTGTCTGGA GGACGGGACA ATCCAAACGA AGAAAGTGGA 
GAAATGTCAA TCGTCATATC TGATGTGCTG CAAGGGGGTC CAGCCGATGG CTTGCTGTTT 
GAAAATGACA GAGTGGTCCA GGTCAACACT GTGCCGATGG ACGGTGTCCC ACACTCCTTT 
GCAGTCCAGA CTCTCCGCAA ATGTGGCAAA GTTGCCAAAA TTACTGTAAA GCGATGTAGA 
AAAATCCCTG TCAACCTGAC CAAGCGTGCT GCATCCCCTG ATGACCGTGT CTTCAGTGGC 
GACTACAACG ATGATTATGA CTATGAACAG GACCAGCACA GCATCTACAG TGGACGCAGC 
TACCCTAACC GTGATACTAG TTTAGAGCGT GAGCGCGGCG GCTATGCTGA CGAGCGTGAA 
CGGGACTATG ATCGCAGTCG GGGGAGGAGC ATGGAGCGCG GCGTGAGCCC TGATAGACAA 
TACAGGCGGG ACGGCAGCCG CGGACGAATG CTGGACAACG AGCGCTGCCC CGACCCCCGT 
TACAGAAGTG AAAATACCTT GGATCGCGAC CGCAGTCCAG ACCCCCGTTA TCGAAGTGAA 
CGCACGCTGG ACCGCAACTA CAGCCCAGAG CGAGGTCGCC GCTTTCGCAG CGAACACAAC 
CTGGACCGAG AGCGAAGTCC AGACACACAA TACCGCAGCG ATCGCACACT GGACAGAACA 
CACAGTCCAG ATACACACTA TAGGCCTGAA CCTGCTCGGG GGTACAGCAG AGAGAATCTC 
ACCAGTGACA ATGAGCGCAG GAGGTATGGC TCACATCAGA ACGAACCCAT GAAGAAGAGC 
AACAGCCAAG ACAGATTGGA TCGTTCGCCT TCACCTGCAC CACTCCGACA CAATGCCTTG 
GAAAAGCCAC TCAGTGTTAC TCTCCTCAAA AACCGTCCCA ATGATGAATA TGGCCTTCGT 
CTAGGCAGTC AGTTGTTCAT CAAAGAGATG ACGAACACAG GCCTTGCATC CAGGGAAGGC 
AAACTACAAG AAGGAGACAT TATTCTGAAA ATCAACGGTA CGGTCACAGA GAATCTGTCT 
CTGAGCGATG CAGGGAAGCT GATTGAGAAG TCTCGTGGAA AGCTGCAGCT GGTGGTGCAG 
AGGGATCGCA GTCAAGTGCT CATCCGCATC CCACCAATGG CAGACAGCGA CTCGGAAATG 
GATGACATCT CTGAGATCGA GTCGTACCGC TCATATTCTC CTCAGGAGGA TCGGCGGAGT 
CATCACTCAG ACCTCTCCTC ACACTCCTCC AATGAGAGAT TACAGGACAA AAACAGAGAA 
GAGCCTCCTA AAAGACTGGC TAAAATGGGA GCGATGCCCA CTCCATTTGT ATCAGCACCG 
GTGGATCTGC CACCTCCACC TGTAGAGAAA GAAGAGCCTC GCAGCGAGTC TCCAGCTCCT 
CCTGTGGTAA TTGCTGCTCC AAAAACTGTT CCAGTAAAAG CAAAACCTTT GCCAAAGGTT 
CCCTTGCTAC CCAGTCCAGA AGAGCAGGAA ATTTATGGGC CCAACACTGT GATGGTGCGC 
TTTGTGAAGG GTGAAAGCGT TGGTCTGCGT CTGGCTGGAG GAAATGATGT GGGCATTTTC 
ATCGCCGGCG TTCAAGAGGA CAGTCCTGCC GAGGTGGAGG GTCTGCGCAC TGGAGACCAA 
ATAGTCAAGG TGAATAACAT GGATTTTCGA GGCATGGTCC GTGAAGATGC AGTTCTGTAT 
TTACTGGAGA TTCCCAAAGG AGAGGATGTG ACCATTTTGG CTCAAAGCAA ACCCGATGTG 
TATAAAGACA TCCTGGGGTC TGGACGAGGT GATAACTTCT TCATCAGGAC TCATTTTGAA 
TATGAGAAAG AGCTTCCCCA GAGCCTGAAC TTCTCTAGAG GAGAGGTTTT TAAAGTGGTG 
GACACCCTGT ATGATGGCAA GCTGGGAAAC TGGCTCGCCA TCCGCATGGA CAAAGACAAT 
CAGCTCCTGG AAAAGGGCAT CATACCCAGC AAGAGCAGGG CGGAGCAGAT GGCAAATGTT 
CAGCATGCAC AGAAAGGTGC TGGTAATGAC AGAGGCGATT TCTGGAGGCT CAGAGGTCAA 
AGGGCAGCTA AGAAAAAAGA CCTTCGCAAG AGCAGAGAGG ATCTCAGTGC AGCTCCAGTC 
ACCACACGCT TCCCGGCCTA TGAGAGAGTG GTGCTGCGTG AAGCTGGCTT CAGGCGTCCA 
GTCGTGATTT TTGGCCCCAT TTCAGATGCA GCCAACGAAA AACTTGCCAA TGAACTTCCA 
AATGAGTTCA CCATTGCAAA GACCGAACCA AAAGACGCAG GCTCAGAAAA GTCATCTGGC 
GTTGTGAGGC TAAACACCAT TCGTCAGATC ATCGAACAGG ATCTACATGC TCTGCTGGAT 
GTCACTCCAA AGGCTGTAGA CACCCTCAAT TACACTCAGT GGTATCCCAT TGTCATCTTC 
CTCAACCCAG ACAGCAAGCA GGGGGTGAAG ACCATGAGGA ATCGCTTGGT GCCTGGATCC 
AACCGCAGCT CACGCAAACT TTACGAACAA GCGGTCAAAC TGCGGAAGAC ATATGCGCAT 
CTGTTCACAG CTACCATTGA TCTGAACTCT TCTCACGATG CCTGGTTTGG AAGCCTCAAA 
GACGCCGTAC GAGAACAGCA AGATAAAGCT GTCTGGGTCT GTCAAAGCAA GCTGGATGGC 
TCAGAGGAGG ACCTGGATCT CCATGATGAC CGTATGTCCT ACCTGTCGGC CATGAGTGCA 
GATTACCTGA GCATGGACAG CCGTCTGACC AGCGACTATG AGGACACGGC GGACGAGGGC 
GGCGCATACA CTGATAATGA GCTTGATGAG TCCACAGATG AGCCTCAGCC AATGTCTGCC 
ATCAGCCGCT CATCTGAACC CGTCAGTGAG GAGAGGCACA TGCCTGTTCC CCACGAGCGC 
TCAAAGAAAC CATCGGGCAG AGACGTCCAG CGTGACCCCA GTCCTCCTCC GTCATTTGTC 
CCAGAACCCC CAAAGGTGCG CTCTGCTTCC CGTCCTGCAG ACTCCAGGAG TTTTGACTCG 
CGCTCCAGCA GCACCATCAG CAGTGATGCG CCCATCAGCA ACAAACCTCT TCCTCCGCCC 
GTCGCCCAGA AGCCCAGTTT CAATACGAGG TCGGCGGACG ACACACCTTC TAATGACCCA 
GCAGATGACC CTGCCAACCG CACCTTCAGG GGAAAGGTGA AGGCCTTCGA GCAGATGGAC 
CACCTTGCCA GAGCCAAACG TATGCTGGAG CTGCAGGAGG CCGAACAAGC CCGGCTGGAA 
ATCTCTCAGA AACATCCAGA TATTTATGCA GTTCCTAACA AGCCAAAACC AAACCAGAAC 
AGACCACAAC CAATTGGCTC CAGCTCAAAC TCCGAGTCCC AGAGCTCCTC CAAAGCAATG 
TATTCAGAGA GAGGCTCACA CTACCGGCAC GATGATGATG ACGAAGAGGA GTATCGCCGC 
CAGCTGGCGG ACCAGACCCG GAGAGGATAT TACAGCACAC AGAAATACAA TGACACAGAG 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188499.1
CDS	145..3810
Translation	MPVVVEKRQFLCRNVKDCYYNPVMEETVWEQYTVTLQRDSKMGFGLAVSGGRDNPNEESGEMSIVISDVLQGGPADGLLFENDRVVQVNTVPMDGVPHSFAVQTLRKCGKVAKITVKRCRKIPVNLTKRAASPDDRVFSGDYNDDYDYEQDQHSIYSGRSYPNRDTSLERERGGYADERERDYDRSRGRSMERGVSPDRQYRRDGSRGRMLDNERCPDPRYRSENTLDRDRSPDPRYRSERTLDRNYSPERGRRFRSEHNLDRERSPDTQYRSDRTLDRTHSPDTHYRPEPARGYSRENLTSDNERRRYGSHQNEPMKKSNSQDRLDRSPSPAPLRHNALEKPLSVTLLKNRPNDEYGLRLGSQLFIKEMTNTGLASREGKLQEGDIILKINGTVTENLSLSDAGKLIEKSRGKLQLVVQRDRSQVLIRIPPMADSDSEMDDISEIESYRSYSPQEDRRSHHSDLSSHSSNERLQDKNREEPPKRLAKMGAMPTPFVSAPVDLPPPPVEKEEPRSESPAPPVVIAAPKTVPVKAKPLPKVPLLPSPEEQEIYGPNTVMVRFVKGESVGLRLAGGNDVGIFIAGVQEDSPAEVEGLRTGDQIVKVNNMDFRGMVREDAVLYLLEIPKGEDVTILAQSKPDVYKDILGSGRGDNFFIRTHFEYEKELPQSLNFSRGEVFKVVDTLYDGKLGNWLAIRMDKDNQLLEKGIIPSKSRAEQMANVQHAQKGAGNDRGDFWRLRGQRAAKKKDLRKSREDLSAAPVTTRFPAYERVVLREAGFRRPVVIFGPISDAANEKLANELPNEFTIAKTEPKDAGSEKSSGVVRLNTIRQIIEQDLHALLDVTPKAVDTLNYTQWYPIVIFLNPDSKQGVKTMRNRLVPGSNRSSRKLYEQAVKLRKTYAHLFTATIDLNSSHDAWFGSLKDAVREQQDKAVWVCQSKLDGSEEDLDLHDDRMSYLSAMSADYLSMDSRLTSDYEDTADEGGAYTDNELDESTDEPQPMSAISRSSEPVSEERHMPVPHERSKKPSGRDVQRDPSPPPSFVPEPPKVRSASRPADSRSFDSRSSSTISSDAPISNKPLPPPVAQKPSFNTRSADDTPSNDPADDPANRTFRGKVKAFEQMDHLARAKRMLELQEAEQARLEISQKHPDIYAVPNKPKPNQNRPQPIGSSSNSESQSSSKAMYSERGSHYRHDDDDEEEYRRQLADQTRRGYYSTQKYNDTEL

Target ORF information:

RefSeq Version	NM_001201570.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201570.1

ORF Insert Sequence:

ATGCCCGTCG TTGTGGAGAA ACGGCAGTTT CTGTGTAGAA ATGTTAAGGA CTGTTATTAT 
AACCCAGTCA TGGAGGAGAC TGTGTGGGAG CAGTACACTG TGACCCTACA GCGGGACTCC 
AAGATGGGCT TTGGCCTAGC GGTGTCTGGA GGACGGGACA ATCCAAACGA AGAAAGTGGA 
GAAATGTCAA TCGTCATATC TGATGTGCTG CAAGGGGGTC CAGCCGATGG CTTGCTGTTT 
GAAAATGACA GAGTGGTCCA GGTCAACACT GTGCCGATGG ACGGTGTCCC ACACTCCTTT 
GCAGTCCAGA CTCTCCGCAA ATGTGGCAAA GTTGCCAAAA TTACTGTAAA GCGATGTAGA 
AAAATCCCTG TCAACCTGAC CAAGCGTGCT GCATCCCCTG ATGACCGTGT CTTCAGTGGC 
GACTACAACG ATGATTATGA CTATGAACAG GACCAGCACA GCATCTACAG TGGACGCAGC 
TACCCTAACC GTGATACTAG TTTAGAGCGT GAGCGCGGCG GCTATGCTGA CGAGCGTGAA 
CGGGACTATG ATCGCAGTCG GGGGAGGAGC ATGGAGCGCG GCGTGAGCCC TGATAGACAA 
TACAGGCGGG ACGGCAGCCG CGGACGAATG CTGGACAACG AGCGCTGCCC CGACCCCCGT 
TACAGAAGTG AAAATACCTT GGATCGCGAC CGCAGTCCAG ACCCCCGTTA TCGAAGTGAA 
CGCACGCTGG ACCGCAACTA CAGCCCAGAG CGAGGTCGCC GCTTTCGCAG CGAACACAAC 
CTGGACCGAG AGCGAAGTCC AGACACACAA TACCGCAGCG ATCGCACACT GGACAGAACA 
CACAGTCCAG ATACACACTA TAGGCCTGAA CCTGCTCGGG GGTACAGCAG AGAGAATCTC 
ACCAGTGACA ATGAGCGCAG GAGGTATGGC TCACATCAGA ACGAACCCAT GAAGAAGAGC 
AACAGCCAAG ACAGATTGGA TCGTTCGCCT TCACCTGCAC CACTCCGACA CAATGCCTTG 
GAAAAGCCAC TCAGTGTTAC TCTCCTCAAA AACCGTCCCA ATGATGAATA TGGCCTTCGT 
CTAGGCAGTC AGTTGTTCAT CAAAGAGATG ACGAACACAG GCCTTGCATC CAGGGAAGGC 
AAACTACAAG AAGGAGACAT TATTCTGAAA ATCAACGGTA CGGTCACAGA GAATCTGTCT 
CTGAGCGATG CAGGGAAGCT GATTGAGAAG TCTCGTGGAA AGCTGCAGCT GGTGGTGCAG 
AGGGATCGCA GTCAAGTGCT CATCCGCATC CCACCAATGG CAGACAGCGA CTCGGAAATG 
GATGACATCT CTGAGATCGA GTCGTACCGC TCATATTCTC CTCAGGAGGA TCGGCGGAGT 
CATCACTCAG ACCTCTCCTC ACACTCCTCC AATGAGAGAT TACAGGACAA AAACAGAGAA 
GAGCCTCCTA AAAGACTGGC TAAAATGGGA GCGATGCCCA CTCCATTTGT ATCAGCACCG 
GTGGATCTGC CACCTCCACC TGTAGAGAAA GAAGAGCCTC GCAGCGAGTC TCCAGCTCCT 
CCTGTGGTAA TTGCTGCTCC AAAAACTGTT CCAGTAAAAG CAAAACCTTT GCCAAAGGTT 
CCCTTGCTAC CCAGTCCAGA AGAGCAGGAA ATTTATGGGC CCAACACTGT GATGGTGCGC 
TTTGTGAAGG GTGAAAGCGT TGGTCTGCGT CTGGCTGGAG GAAATGATGT GGGCATTTTC 
ATCGCCGGCG TTCAAGAGGA CAGTCCTGCC GAGGTGGAGG GTCTGCGCAC TGGAGACCAA 
ATAGTCAAGG TGAATAACAT GGATTTTCGA GGCATGGTCC GTGAAGATGC AGTTCTGTAT 
TTACTGGAGA TTCCCAAAGG AGAGGATGTG ACCATTTTGG CTCAAAGCAA ACCCGATGTG 
TATAAAGACA TCCTGGGGTC TGGACGAGGT GATAACTTCT TCATCAGGAC TCATTTTGAA 
TATGAGAAAG AGCTTCCCCA GAGCCTGAAC TTCTCTAGAG GAGAGGTTTT TAAAGTGGTG 
GACACCCTGT ATGATGGCAA GCTGGGAAAC TGGCTCGCCA TCCGCATGGA CAAAGACAAT 
CAGCTCCTGG AAAAGGGCAT CATACCCAGC AAGAGCAGGG CGGAGCAGAT GGCAAATGTT 
CAGCATGCAC AGAAAGGTGC TGGTAATGAC AGAGGCGATT TCTGGAGGCT CAGAGGTCAA 
AGGGCAGCTA AGAAAAAAGA CCTTCGCAAG AGCAGAGAGG ATCTCAGTGC AGCTCCAGTC 
ACCACACGCT TCCCGGCCTA TGAGAGAGTG GTGCTGCGTG AAGCTGGCTT CAGGCGTCCA 
GTCGTGATTT TTGGCCCCAT TTCAGATGCA GCCAACGAAA AACTTGCCAA TGAACTTCCA 
AATGAGTTCA CCATTGCAAA GACCGAACCA AAAGACGCAG GCTCAGAAAA GTCATCTGGC 
GTTGTGAGGC TAAACACCAT TCGTCAGATC ATCGAACAGG ATCTACATGC TCTGCTGGAT 
GTCACTCCAA AGGCTGTAGA CACCCTCAAT TACACTCAGT GGTATCCCAT TGTCATCTTC 
CTCAACCCAG ACAGCAAGCA GGGGGTGAAG ACCATGAGGA ATCGCTTGGT GCCTGGATCC 
AACCGCAGCT CACGCAAACT TTACGAACAA GCGGTCAAAC TGCGGAAGAC ATATGCGCAT 
CTGTTCACAG CTACCATTGA TCTGAACTCT TCTCACGATG CCTGGTTTGG AAGCCTCAAA 
GACGCCGTAC GAGAACAGCA AGATAAAGCT GTCTGGGTCT GTCAAAGCAA GCTGGATGGC 
TCAGAGGAGG ACCTGGATCT CCATGATGAC CGTATGTCCT ACCTGTCGGC CATGAGTGCA 
GATTACCTGA GCATGGACAG CCGTCTGACC AGCGACTATG AGGACACGGC GGACGAGGGC 
GGCGCATACA CTGATAATGA GCTTGATGAG TCCACAGATG AGCCTCAGCC AATGTCTGCC 
ATCAGCCGCT CATCTGAACC CGTCAGTGAG GAGAGGCACA TGCCTGTTCC CCACGAGCGC 
TCAAAGAAAC CATCGGGCAG AGACGTCCAG CGTGACCCCA GTCCTCCTCC GTCATTTGTC 
CCAGAACCCC CAAAGGTGCG CTCTGCTTCC CGTCCTGCAG ACTCCAGGAG TTTTGACTCG 
CGCTCCAGCA GCACCATCAG CAGTGATGCG CCCATCAGCA ACAAACCTCT TCCTCCGCCC 
GTCGCCCAGA AGCCCAGTTT CAATACGAGG TCGGCGGACG ACACACCTTC TAATGACCCA 
GCAGATGACC CTGCCAACCG CACCTTCAGG GGAAAGGTGA AGGCCTTCGA GCAGATGGAC 
CACCTTGCCA GAGCCAAACG TATGCTGGAG CTGCAGGAGG CCGAACAAGC CCGGCTGGAA 
ATCTCTCAGA AACATCCAGA TATTTATGCA GTTCCTAACA AGCCAAAACC AAACCAGAAC 
AGACCACAAC CAATTGGCTC CAGCTCAAAC TCCGAGTCCC AGAGCTCCTC CAAAGCAATG 
TATTCAGAGA GAGGCTCACA CTACCGGCAC GATGATGATG ACGAAGAGGA GTATCGCCGC 
CAGCTGGCGG ACCAGACCCG GAGAGGATAT TACAGCACAC AGAAATACAA TGACACAGAG 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP2A ( NM_001201571.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001188500.1 Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa35091
Clone ID Related Accession (Same CDS sequence)	NM_001201571.1
Accession Version	NM_001201571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX640481.14 and BX629350.6. On Jan 28, 2011 this sequence version replaced XM_688726.4. Transcript Variant: This variant (2) lacks the first 5' exon, includes two non-coding 5' exons and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01002488.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA CTGTGTGGGA GCAGTACACT GTGACCCTAC AGCGGGACTC CAAGATGGGC 
TTTGGCCTAG CGGTGTCTGG AGGACGGGAC AATCCAAACG AAGAAAGTGG AGAAATGTCA 
ATCGTCATAT CTGATGTGCT GCAAGGGGGT CCAGCCGATG GCTTGCTGTT TGAAAATGAC 
AGAGTGGTCC AGGTCAACAC TGTGCCGATG GACGGTGTCC CACACTCCTT TGCAGTCCAG 
ACTCTCCGCA AATGTGGCAA AGTTGCCAAA ATTACTGTAA AGCGATGTAG AAAAATCCCT 
GTCAACCTGA CCAAGCGTGC TGCATCCCCT GATGACCGTG TCTTCAGTGG CGACTACAAC 
GATGATTATG ACTATGAACA GGACCAGCAC AGCATCTACA GTGGACGCAG CTACCCTAAC 
CGTGATACTA GTTTAGAGCG TGAGCGCGGC GGCTATGCTG ACGAGCGTGA ACGGGACTAT 
GATCGCAGTC GGGGGAGGAG CATGGAGCGC GGCGTGAGCC CTGATAGACA ATACAGGCGG 
GACGGCAGCC GCGGACGAAT GCTGGACAAC GAGCGCTGCC CCGACCCCCG TTACAGAAGT 
GAAAATACCT TGGATCGCGA CCGCAGTCCA GACCCCCGTT ATCGAAGTGA ACGCACGCTG 
GACCGCAACT ACAGCCCAGA GCGAGGTCGC CGCTTTCGCA GCGAACACAA CCTGGACCGA 
GAGCGAAGTC CAGACACACA ATACCGCAGC GATCGCACAC TGGACAGAAC ACACAGTCCA 
GATACACACT ATAGGCCTGA ACCTGCTCGG GGGTACAGCA GAGAGAATCT CACCAGTGAC 
AATGAGCGCA GGAGGTATGG CTCACATCAG AACGAACCCA TGAAGAAGAG CAACAGCCAA 
GACAGATTGG ATCGTTCGCC TTCACCTGCA CCACTCCGAC ACAATGCCTT GGAAAAGCCA 
CTCAGTGTTA CTCTCCTCAA AAACCGTCCC AATGATGAAT ATGGCCTTCG TCTAGGCAGT 
CAGTTGTTCA TCAAAGAGAT GACGAACACA GGCCTTGCAT CCAGGGAAGG CAAACTACAA 
GAAGGAGACA TTATTCTGAA AATCAACGGT ACGGTCACAG AGAATCTGTC TCTGAGCGAT 
GCAGGGAAGC TGATTGAGAA GTCTCGTGGA AAGCTGCAGC TGGTGGTGCA GAGGGATCGC 
AGTCAAGTGC TCATCCGCAT CCCACCAATG GCAGACAGCG ACTCGGAAAT GGATGACATC 
TCTGAGATCG AGTCGTACCG CTCATATTCT CCTCAGGAGG ATCGGCGGAG TCATCACTCA 
GACCTCTCCT CACACTCCTC CAATGAGAGA TTACAGGACA AAAACAGAGA AGAGCCTCCT 
AAAAGACTGG CTAAAATGGG AGCGATGCCC ACTCCATTTG TATCAGCACC GGTGGATCTG 
CCACCTCCAC CTGTAGAGAA AGAAGAGCCT CGCAGCGAGT CTCCAGCTCC TCCTGTGGTA 
ATTGCTGCTC CAAAAACTGT TCCAGTAAAA GCAAAACCTT TGCCAAAGGT TCCCTTGCTA 
CCCAGTCCAG AAGAGCAGGA AATTTATGGG CCCAACACTG TGATGGTGCG CTTTGTGAAG 
GGTGAAAGCG TTGGTCTGCG TCTGGCTGGA GGAAATGATG TGGGCATTTT CATCGCCGGC 
GTTCAAGAGG ACAGTCCTGC CGAGGTGGAG GGTCTGCGCA CTGGAGACCA AATAGTCAAG 
GTGAATAACA TGGATTTTCG AGGCATGGTC CGTGAAGATG CAGTTCTGTA TTTACTGGAG 
ATTCCCAAAG GAGAGGATGT GACCATTTTG GCTCAAAGCA AACCCGATGT GTATAAAGAC 
ATCCTGGGGT CTGGACGAGG TGATAACTTC TTCATCAGGA CTCATTTTGA ATATGAGAAA 
GAGCTTCCCC AGAGCCTGAA CTTCTCTAGA GGAGAGGTTT TTAAAGTGGT GGACACCCTG 
TATGATGGCA AGCTGGGAAA CTGGCTCGCC ATCCGCATGG ACAAAGACAA TCAGCTCCTG 
GAAAAGGGCA TCATACCCAG CAAGAGCAGG GCGGAGCAGA TGGCAAATGT TCAGCATGCA 
CAGAAAGGTG CTGGTAATGA CAGAGGCGAT TTCTGGAGGC TCAGAGGTCA AAGGGCAGCT 
AAGAAAAAAG ACCTTCGCAA GAGCAGAGAG GATCTCAGTG CAGCTCCAGT CACCACACGC 
TTCCCGGCCT ATGAGAGAGT GGTGCTGCGT GAAGCTGGCT TCAGGCGTCC AGTCGTGATT 
TTTGGCCCCA TTTCAGATGC AGCCAACGAA AAACTTGCCA ATGAACTTCC AAATGAGTTC 
ACCATTGCAA AGACCGAACC AAAAGACGCA GGCTCAGAAA AGTCATCTGG CGTTGTGAGG 
CTAAACACCA TTCGTCAGAT CATCGAACAG GATCTACATG CTCTGCTGGA TGTCACTCCA 
AAGGCTGTAG ACACCCTCAA TTACACTCAG TGGTATCCCA TTGTCATCTT CCTCAACCCA 
GACAGCAAGC AGGGGGTGAA GACCATGAGG AATCGCTTGG TGCCTGGATC CAACCGCAGC 
TCACGCAAAC TTTACGAACA AGCGGTCAAA CTGCGGAAGA CATATGCGCA TCTGTTCACA 
GCTACCATTG ATCTGAACTC TTCTCACGAT GCCTGGTTTG GAAGCCTCAA AGACGCCGTA 
CGAGAACAGC AAGATAAAGC TGTCTGGGTC TGTCAAAGCA AGCTGGATGG CTCAGAGGAG 
GACCTGGATC TCCATGATGA CCGTATGTCC TACCTGTCGG CCATGAGTGC AGATTACCTG 
AGCATGGACA GCCGTCTGAC CAGCGACTAT GAGGACACGG CGGACGAGGG CGGCGCATAC 
ACTGATAATG AGCTTGATGA GTCCACAGAT GAGCCTCAGC CAATGTCTGC CATCAGCCGC 
TCATCTGAAC CCGTCAGTGA GGAGAGGCAC ATGCCTGTTC CCCACGAGCG CTCAAAGAAA 
CCATCGGGCA GAGACGTCCA GCGTGACCCC AGTCCTCCTC CGTCATTTGT CCCAGAACCC 
CCAAAGGTGC GCTCTGCTTC CCGTCCTGCA GACTCCAGGA GTTTTGACTC GCGCTCCAGC 
AGCACCATCA GCAGTGATGC GCCCATCAGC AACAAACCTC TTCCTCCGCC CGTCGCCCAG 
AAGCCCAGTT TCAATACGAG GTCGGCGGAC GACACACCTT CTAATGACCC AGCAGATGAC 
CCTGCCAACC GCACCTTCAG GGGAAAGGTG AAGGCCTTCG AGCAGATGGA CCACCTTGCC 
AGAGCCAAAC GTATGCTGGA GCTGCAGGAG GCCGAACAAG CCCGGCTGGA AATCTCTCAG 
AAACATCCAG ATATTTATGC AGTTCCTAAC AAGCCAAAAC CAAACCAGAA CAGACCACAA 
CCAATTGGCT CCAGCTCAAA CTCCGAGTCC CAGAGCTCCT CCAAAGCAAT GTATTCAGAG 
AGAGGCTCAC ACTACCGGCA CGATGATGAT GACGAAGAGG AGTATCGCCG CCAGCTGGCG 
GACCAGACCC GGAGAGGATA TTACAGCACA CAGAAATACA ATGACACAGA GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188500.1
CDS	310..3906
Translation	MEETVWEQYTVTLQRDSKMGFGLAVSGGRDNPNEESGEMSIVISDVLQGGPADGLLFENDRVVQVNTVPMDGVPHSFAVQTLRKCGKVAKITVKRCRKIPVNLTKRAASPDDRVFSGDYNDDYDYEQDQHSIYSGRSYPNRDTSLERERGGYADERERDYDRSRGRSMERGVSPDRQYRRDGSRGRMLDNERCPDPRYRSENTLDRDRSPDPRYRSERTLDRNYSPERGRRFRSEHNLDRERSPDTQYRSDRTLDRTHSPDTHYRPEPARGYSRENLTSDNERRRYGSHQNEPMKKSNSQDRLDRSPSPAPLRHNALEKPLSVTLLKNRPNDEYGLRLGSQLFIKEMTNTGLASREGKLQEGDIILKINGTVTENLSLSDAGKLIEKSRGKLQLVVQRDRSQVLIRIPPMADSDSEMDDISEIESYRSYSPQEDRRSHHSDLSSHSSNERLQDKNREEPPKRLAKMGAMPTPFVSAPVDLPPPPVEKEEPRSESPAPPVVIAAPKTVPVKAKPLPKVPLLPSPEEQEIYGPNTVMVRFVKGESVGLRLAGGNDVGIFIAGVQEDSPAEVEGLRTGDQIVKVNNMDFRGMVREDAVLYLLEIPKGEDVTILAQSKPDVYKDILGSGRGDNFFIRTHFEYEKELPQSLNFSRGEVFKVVDTLYDGKLGNWLAIRMDKDNQLLEKGIIPSKSRAEQMANVQHAQKGAGNDRGDFWRLRGQRAAKKKDLRKSREDLSAAPVTTRFPAYERVVLREAGFRRPVVIFGPISDAANEKLANELPNEFTIAKTEPKDAGSEKSSGVVRLNTIRQIIEQDLHALLDVTPKAVDTLNYTQWYPIVIFLNPDSKQGVKTMRNRLVPGSNRSSRKLYEQAVKLRKTYAHLFTATIDLNSSHDAWFGSLKDAVREQQDKAVWVCQSKLDGSEEDLDLHDDRMSYLSAMSADYLSMDSRLTSDYEDTADEGGAYTDNELDESTDEPQPMSAISRSSEPVSEERHMPVPHERSKKPSGRDVQRDPSPPPSFVPEPPKVRSASRPADSRSFDSRSSSTISSDAPISNKPLPPPVAQKPSFNTRSADDTPSNDPADDPANRTFRGKVKAFEQMDHLARAKRMLELQEAEQARLEISQKHPDIYAVPNKPKPNQNRPQPIGSSSNSESQSSSKAMYSERGSHYRHDDDDEEEYRRQLADQTRRGYYSTQKYNDTEL

Target ORF information:

RefSeq Version	NM_001201571.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201571.1

ORF Insert Sequence:

ATGGAGGAGA CTGTGTGGGA GCAGTACACT GTGACCCTAC AGCGGGACTC CAAGATGGGC 
TTTGGCCTAG CGGTGTCTGG AGGACGGGAC AATCCAAACG AAGAAAGTGG AGAAATGTCA 
ATCGTCATAT CTGATGTGCT GCAAGGGGGT CCAGCCGATG GCTTGCTGTT TGAAAATGAC 
AGAGTGGTCC AGGTCAACAC TGTGCCGATG GACGGTGTCC CACACTCCTT TGCAGTCCAG 
ACTCTCCGCA AATGTGGCAA AGTTGCCAAA ATTACTGTAA AGCGATGTAG AAAAATCCCT 
GTCAACCTGA CCAAGCGTGC TGCATCCCCT GATGACCGTG TCTTCAGTGG CGACTACAAC 
GATGATTATG ACTATGAACA GGACCAGCAC AGCATCTACA GTGGACGCAG CTACCCTAAC 
CGTGATACTA GTTTAGAGCG TGAGCGCGGC GGCTATGCTG ACGAGCGTGA ACGGGACTAT 
GATCGCAGTC GGGGGAGGAG CATGGAGCGC GGCGTGAGCC CTGATAGACA ATACAGGCGG 
GACGGCAGCC GCGGACGAAT GCTGGACAAC GAGCGCTGCC CCGACCCCCG TTACAGAAGT 
GAAAATACCT TGGATCGCGA CCGCAGTCCA GACCCCCGTT ATCGAAGTGA ACGCACGCTG 
GACCGCAACT ACAGCCCAGA GCGAGGTCGC CGCTTTCGCA GCGAACACAA CCTGGACCGA 
GAGCGAAGTC CAGACACACA ATACCGCAGC GATCGCACAC TGGACAGAAC ACACAGTCCA 
GATACACACT ATAGGCCTGA ACCTGCTCGG GGGTACAGCA GAGAGAATCT CACCAGTGAC 
AATGAGCGCA GGAGGTATGG CTCACATCAG AACGAACCCA TGAAGAAGAG CAACAGCCAA 
GACAGATTGG ATCGTTCGCC TTCACCTGCA CCACTCCGAC ACAATGCCTT GGAAAAGCCA 
CTCAGTGTTA CTCTCCTCAA AAACCGTCCC AATGATGAAT ATGGCCTTCG TCTAGGCAGT 
CAGTTGTTCA TCAAAGAGAT GACGAACACA GGCCTTGCAT CCAGGGAAGG CAAACTACAA 
GAAGGAGACA TTATTCTGAA AATCAACGGT ACGGTCACAG AGAATCTGTC TCTGAGCGAT 
GCAGGGAAGC TGATTGAGAA GTCTCGTGGA AAGCTGCAGC TGGTGGTGCA GAGGGATCGC 
AGTCAAGTGC TCATCCGCAT CCCACCAATG GCAGACAGCG ACTCGGAAAT GGATGACATC 
TCTGAGATCG AGTCGTACCG CTCATATTCT CCTCAGGAGG ATCGGCGGAG TCATCACTCA 
GACCTCTCCT CACACTCCTC CAATGAGAGA TTACAGGACA AAAACAGAGA AGAGCCTCCT 
AAAAGACTGG CTAAAATGGG AGCGATGCCC ACTCCATTTG TATCAGCACC GGTGGATCTG 
CCACCTCCAC CTGTAGAGAA AGAAGAGCCT CGCAGCGAGT CTCCAGCTCC TCCTGTGGTA 
ATTGCTGCTC CAAAAACTGT TCCAGTAAAA GCAAAACCTT TGCCAAAGGT TCCCTTGCTA 
CCCAGTCCAG AAGAGCAGGA AATTTATGGG CCCAACACTG TGATGGTGCG CTTTGTGAAG 
GGTGAAAGCG TTGGTCTGCG TCTGGCTGGA GGAAATGATG TGGGCATTTT CATCGCCGGC 
GTTCAAGAGG ACAGTCCTGC CGAGGTGGAG GGTCTGCGCA CTGGAGACCA AATAGTCAAG 
GTGAATAACA TGGATTTTCG AGGCATGGTC CGTGAAGATG CAGTTCTGTA TTTACTGGAG 
ATTCCCAAAG GAGAGGATGT GACCATTTTG GCTCAAAGCA AACCCGATGT GTATAAAGAC 
ATCCTGGGGT CTGGACGAGG TGATAACTTC TTCATCAGGA CTCATTTTGA ATATGAGAAA 
GAGCTTCCCC AGAGCCTGAA CTTCTCTAGA GGAGAGGTTT TTAAAGTGGT GGACACCCTG 
TATGATGGCA AGCTGGGAAA CTGGCTCGCC ATCCGCATGG ACAAAGACAA TCAGCTCCTG 
GAAAAGGGCA TCATACCCAG CAAGAGCAGG GCGGAGCAGA TGGCAAATGT TCAGCATGCA 
CAGAAAGGTG CTGGTAATGA CAGAGGCGAT TTCTGGAGGC TCAGAGGTCA AAGGGCAGCT 
AAGAAAAAAG ACCTTCGCAA GAGCAGAGAG GATCTCAGTG CAGCTCCAGT CACCACACGC 
TTCCCGGCCT ATGAGAGAGT GGTGCTGCGT GAAGCTGGCT TCAGGCGTCC AGTCGTGATT 
TTTGGCCCCA TTTCAGATGC AGCCAACGAA AAACTTGCCA ATGAACTTCC AAATGAGTTC 
ACCATTGCAA AGACCGAACC AAAAGACGCA GGCTCAGAAA AGTCATCTGG CGTTGTGAGG 
CTAAACACCA TTCGTCAGAT CATCGAACAG GATCTACATG CTCTGCTGGA TGTCACTCCA 
AAGGCTGTAG ACACCCTCAA TTACACTCAG TGGTATCCCA TTGTCATCTT CCTCAACCCA 
GACAGCAAGC AGGGGGTGAA GACCATGAGG AATCGCTTGG TGCCTGGATC CAACCGCAGC 
TCACGCAAAC TTTACGAACA AGCGGTCAAA CTGCGGAAGA CATATGCGCA TCTGTTCACA 
GCTACCATTG ATCTGAACTC TTCTCACGAT GCCTGGTTTG GAAGCCTCAA AGACGCCGTA 
CGAGAACAGC AAGATAAAGC TGTCTGGGTC TGTCAAAGCA AGCTGGATGG CTCAGAGGAG 
GACCTGGATC TCCATGATGA CCGTATGTCC TACCTGTCGG CCATGAGTGC AGATTACCTG 
AGCATGGACA GCCGTCTGAC CAGCGACTAT GAGGACACGG CGGACGAGGG CGGCGCATAC 
ACTGATAATG AGCTTGATGA GTCCACAGAT GAGCCTCAGC CAATGTCTGC CATCAGCCGC 
TCATCTGAAC CCGTCAGTGA GGAGAGGCAC ATGCCTGTTC CCCACGAGCG CTCAAAGAAA 
CCATCGGGCA GAGACGTCCA GCGTGACCCC AGTCCTCCTC CGTCATTTGT CCCAGAACCC 
CCAAAGGTGC GCTCTGCTTC CCGTCCTGCA GACTCCAGGA GTTTTGACTC GCGCTCCAGC 
AGCACCATCA GCAGTGATGC GCCCATCAGC AACAAACCTC TTCCTCCGCC CGTCGCCCAG 
AAGCCCAGTT TCAATACGAG GTCGGCGGAC GACACACCTT CTAATGACCC AGCAGATGAC 
CCTGCCAACC GCACCTTCAG GGGAAAGGTG AAGGCCTTCG AGCAGATGGA CCACCTTGCC 
AGAGCCAAAC GTATGCTGGA GCTGCAGGAG GCCGAACAAG CCCGGCTGGA AATCTCTCAG 
AAACATCCAG ATATTTATGC AGTTCCTAAC AAGCCAAAAC CAAACCAGAA CAGACCACAA 
CCAATTGGCT CCAGCTCAAA CTCCGAGTCC CAGAGCTCCT CCAAAGCAAT GTATTCAGAG 
AGAGGCTCAC ACTACCGGCA CGATGATGAT GACGAAGAGG AGTATCGCCG CCAGCTGGCG 
GACCAGACCC GGAGAGGATA TTACAGCACA CAGAAATACA ATGACACAGA GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP2A ( XM_005155574.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155631.1 Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA.

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CloneID	ODa15901
Clone ID Related Accession (Same CDS sequence)	XM_005155574.4 , XM_005155574.3
Accession Version	XM_005155574.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155574.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGG TCCTCTCGCT GCACAGGAAA TGGGTGCACA CCATGAAGGC CATCCGCATC 
CTCCAGGGCA TCAACCCAGT CATGGAGGAG ACTGTGTGGG AGCAGTACAC TGTGACCCTA 
CAGCGGGACT CCAAGATGGG CTTTGGCCTA GCGGTGTCTG GAGGACGGGA CAATCCAAAC 
GAAGAAAGTG GAGAAATGTC AATCGTCATA TCTGATGTGC TGCAAGGGGG TCCAGCCGAT 
GGCTTGCTGT TTGAAAATGA CAGAGTGGTC CAGGTCAACA CTGTGCCGAT GGACGGTGTC 
CCACACTCCT TTGCAGTCCA GACTCTCCGC AAATGTGGCA AAGTTGCCAA AATTACTGTA 
AAGCGATGTA GAAAAATCCC TGTCAACCTG ACCAAGCGTG CTGCATCCCC TGATGACCGT 
GTCTTCAGTG GCGACTACAA CGATGATTAT GACTATGAAC AGGACCAGCA CAGCATCTAC 
AGTGGACGCA GCTACCCTAA CCGTGATACT AGTTTAGAGC GTGAGCGCGG CGGCTATGCT 
GACGAGCGTG AACGGGACTA TGATCGCAGT CGGGGGAGGA GCATGGAGCG CGGCGTGAGC 
CCTGATAGAC AATACAGGCG GGACGGCAGC CGCGGACGAA TGCTGGACAA CGAGCGCTGC 
CCCGACCCCC GTTACAGAAG TGAAAATACC TTGGATCGCG ACCGCAGTCC AGACCCCCGT 
TATCGAAGTG AACGCACGCT GGACCGCAAC TACAGCCCAG AGCGAGGTCG CCGCTTTCGC 
AGCGAACACA ACCTGGACCG AGAGCGAAGT CCAGACACAC AATACCGCAG CGATCGCACA 
CTGGACAGAA CACACAGTCC AGATACACAC TATAGGCCTG AACCTGCTCG GGGGTACAGC 
AGAGAGAATC TCACCAGTGA CAATGAGCGC AGGAGGTATG GCTCACATCA GAACGAACCC 
ATGAAGAAGA GCAACAGCCA AGACAGATTG GATCGTTCGC CTTCACCTGC ACCACTCCGA 
CACAATGCCT TGGAAAAGCC ACTCAGTGTT ACTCTCCTCA AAAACCGTCC CAATGATGAA 
TATGGCCTTC GTCTAGGCAG TCAGTTGTTC ATCAAAGAGA TGACGAACAC AGGCCTTGCA 
TCCAGGGAAG GCAAACTACA AGAAGGAGAC ATTATTCTGA AAATCAACGG TACGGTCACA 
GAGAATCTGT CTCTGAGCGA TGCAGGGAAG CTGATTGAGA AGTCTCGTGG AAAGCTGCAG 
CTGGTGGTGC AGAGGGATCG CAGTCAAGTG CTCATCCGCA TCCCACCAAT GGCAGACAGC 
GACTCGGAAA TGGATGACAT CTCTGAGATC GAGTCGTACC GCTCATATTC TCCTCAGGAG 
GATCGGCGGA GTCATCACTC AGACCTCTCC TCACACTCCT CCAATGAGAG ATTACAGGAC 
AAAAACAGAG AAGAGCCTCC TAAAAGACTG GCTAAAATGG GAGCGATGCC CACTCCATTT 
GTATCAGCAC CGGTGGATCT GCCACCTCCA CCTGTAGAGA AAGAAGAGCC TCGCAGCGAG 
TCTCCAGCTC CTCCTGTGGT AATTGCTGCT CCAAAAACTG TTCCAGTAAA AGCAAAACCT 
TTGCCAAAGG TTCCCTTGCT ACCCAGTCCA GAAGAGCAGG AAATTTATGG GCCCAACACT 
GTGATGGTGC GCTTTGTGAA GGGTGAAAGC GTTGGTCTGC GTCTGGCTGG AGGAAATGAT 
GTGGGCATTT TCATCGCCGG CGTTCAAGAG GACAGTCCTG CCGAGGTGGA GGGTCTGCGC 
ACTGGAGACC AAATAGTCAA GGTGAATAAC ATGGATTTTC GAGGCATGGT CCGTGAAGAT 
GCAGTTCTGT ATTTACTGGA GATTCCCAAA GGAGAGGATG TGACCATTTT GGCTCAAAGC 
AAACCCGATG TGTATAAAGA CATCCTGGGG TCTGGACGAG GTGATAACTT CTTCATCAGG 
ACTCATTTTG AATATGAGAA AGAGCTTCCC CAGAGCCTGA ACTTCTCTAG AGGAGAGGTT 
TTTAAAGTGG TGGACACCCT GTATGATGGC AAGCTGGGAA ACTGGCTCGC CATCCGCATG 
GACAAAGACA ATCAGCTCCT GGAAAAGGGC ATCATACCCA GCAAGAGCAG GGCGGAGCAG 
ATGGCAAATG TTCAGCATGC ACAGAAAGGT GCTGGTAATG ACAGAGGCGA TTTCTGGAGG 
CTCAGAGGTC AAAGGGCAGC TAAGAAAAAA GACCTTCGCA AGAGCAGAGA GGATCTCAGT 
GCAGCTCCAG TCACCACACG CTTCCCGGCC TATGAGAGAG TGGTGCTGCG TGAAGCTGGC 
TTCAGGCGTC CAGTCGTGAT TTTTGGCCCC ATTTCAGATG CAGCCAACGA AAAACTTGCC 
AATGAACTTC CAAATGAGTT CACCATTGCA AAGACCGAAC CAAAAGACGC AGGCTCAGAA 
AAGTCATCTG GCGTTGTGAG GCTAAACACC ATTCGTCAGA TCATCGAACA GGATCTACAT 
GCTCTGCTGG ATGTCACTCC AAAGGCTGTA GACACCCTCA ATTACACTCA GTGGTATCCC 
ATTGTCATCT TCCTCAACCC AGACAGCAAG CAGGGGGTGA AGACCATGAG GAATCGCTTG 
GTGCCTGGAT CCAACCGCAG CTCACGCAAA CTTTACGAAC AAGCGGTCAA ACTGCGGAAG 
ACATATGCGC ATCTGTTCAC AGCTACCATT GATCTGAACT CTTCTCACGA TGCCTGGTTT 
GGAAGCCTCA AAGACGCCGT ACGAGAACAG CAAGATAAAG CTGTCTGGGT CTGTCAAAGC 
AAGCTGGATG GCTCAGAGGA GGACCTGGAT CTCCATGATG ACCGTATGTC CTACCTGTCG 
GCCATGAGTG CAGATTACCT GAGCATGGAC AGCCGTCTGA CCAGCGACTA TGAGGACACG 
GCGGACGAGG GCGGCGCATA CACTGATAAT GAGCTTGATG AGTCCACAGA TGAGCCTCAG 
CCAATGTCTG CCATCAGCCG CTCATCTGAA CCCGTCAGTG AGGAGAGGCA CATGCCTGTT 
CCCCACGAGC GCTCAAAGAA ACCATCGGGC AGAGACGTCC AGCGTGACCC CAGTCCTCCT 
CCGTCATTTG TCCCAGAACC CCCAAAGGTG CGCTCTGCTT CCCGTCCTGC AGACTCCAGG 
AGTTTTGACT CGCGCTCCAG CAGCACCATC AGCAGTGATG CGCCCATCAG CAACAAACCT 
CTTCCTCCGC CCGTCGCCCA GAAGCCCAGT TTCAATACGA GGTCGGCGGA CGACACACCT 
TCTAATGACC CAGCAGATGA CCCTGCCAAC CGCACCTTCA GGGGAAAGGT GAAGGCCTTC 
GAGCAGATGG ACCACCTTGC CAGAGCCAAA CGTATGCTGG AGCTGCAGGA GGCCGAACAA 
GCCCGGCTGG AAATCTCTCA GAAACATCCA GATATTTATG CAGTTCCTAA CAAGCCAAAA 
CCAAACCAGA ACAGACCACA ACCAATTGGC TCCAGCTCAA ACTCCGAGTC CCAGAGCTCC 
TCCAAAGCAA TGTATTCAGA GAGAGGCTCA CACTACCGGC ACGATGATGA TGACGAAGAG 
GAGTATCGCC GCCAGCTGGC GGACCAGACC CGGAGAGGAT ATTACAGCAC ACAGAAATAC 
AATGACACAG AGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155631.1
CDS	323..4000
Translation	MKTVLSLHRKWVHTMKAIRILQGINPVMEETVWEQYTVTLQRDSKMGFGLAVSGGRDNPNEESGEMSIVISDVLQGGPADGLLFENDRVVQVNTVPMDGVPHSFAVQTLRKCGKVAKITVKRCRKIPVNLTKRAASPDDRVFSGDYNDDYDYEQDQHSIYSGRSYPNRDTSLERERGGYADERERDYDRSRGRSMERGVSPDRQYRRDGSRGRMLDNERCPDPRYRSENTLDRDRSPDPRYRSERTLDRNYSPERGRRFRSEHNLDRERSPDTQYRSDRTLDRTHSPDTHYRPEPARGYSRENLTSDNERRRYGSHQNEPMKKSNSQDRLDRSPSPAPLRHNALEKPLSVTLLKNRPNDEYGLRLGSQLFIKEMTNTGLASREGKLQEGDIILKINGTVTENLSLSDAGKLIEKSRGKLQLVVQRDRSQVLIRIPPMADSDSEMDDISEIESYRSYSPQEDRRSHHSDLSSHSSNERLQDKNREEPPKRLAKMGAMPTPFVSAPVDLPPPPVEKEEPRSESPAPPVVIAAPKTVPVKAKPLPKVPLLPSPEEQEIYGPNTVMVRFVKGESVGLRLAGGNDVGIFIAGVQEDSPAEVEGLRTGDQIVKVNNMDFRGMVREDAVLYLLEIPKGEDVTILAQSKPDVYKDILGSGRGDNFFIRTHFEYEKELPQSLNFSRGEVFKVVDTLYDGKLGNWLAIRMDKDNQLLEKGIIPSKSRAEQMANVQHAQKGAGNDRGDFWRLRGQRAAKKKDLRKSREDLSAAPVTTRFPAYERVVLREAGFRRPVVIFGPISDAANEKLANELPNEFTIAKTEPKDAGSEKSSGVVRLNTIRQIIEQDLHALLDVTPKAVDTLNYTQWYPIVIFLNPDSKQGVKTMRNRLVPGSNRSSRKLYEQAVKLRKTYAHLFTATIDLNSSHDAWFGSLKDAVREQQDKAVWVCQSKLDGSEEDLDLHDDRMSYLSAMSADYLSMDSRLTSDYEDTADEGGAYTDNELDESTDEPQPMSAISRSSEPVSEERHMPVPHERSKKPSGRDVQRDPSPPPSFVPEPPKVRSASRPADSRSFDSRSSSTISSDAPISNKPLPPPVAQKPSFNTRSADDTPSNDPADDPANRTFRGKVKAFEQMDHLARAKRMLELQEAEQARLEISQKHPDIYAVPNKPKPNQNRPQPIGSSSNSESQSSSKAMYSERGSHYRHDDDDEEEYRRQLADQTRRGYYSTQKYNDTEL

Target ORF information:

RefSeq Version	XM_005155574.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155574.4

ORF Insert Sequence:

ATGAAGACGG TCCTCTCGCT GCACAGGAAA TGGGTGCACA CCATGAAGGC CATCCGCATC 
CTCCAGGGCA TCAACCCAGT CATGGAGGAG ACTGTGTGGG AGCAGTACAC TGTGACCCTA 
CAGCGGGACT CCAAGATGGG CTTTGGCCTA GCGGTGTCTG GAGGACGGGA CAATCCAAAC 
GAAGAAAGTG GAGAAATGTC AATCGTCATA TCTGATGTGC TGCAAGGGGG TCCAGCCGAT 
GGCTTGCTGT TTGAAAATGA CAGAGTGGTC CAGGTCAACA CTGTGCCGAT GGACGGTGTC 
CCACACTCCT TTGCAGTCCA GACTCTCCGC AAATGTGGCA AAGTTGCCAA AATTACTGTA 
AAGCGATGTA GAAAAATCCC TGTCAACCTG ACCAAGCGTG CTGCATCCCC TGATGACCGT 
GTCTTCAGTG GCGACTACAA CGATGATTAT GACTATGAAC AGGACCAGCA CAGCATCTAC 
AGTGGACGCA GCTACCCTAA CCGTGATACT AGTTTAGAGC GTGAGCGCGG CGGCTATGCT 
GACGAGCGTG AACGGGACTA TGATCGCAGT CGGGGGAGGA GCATGGAGCG CGGCGTGAGC 
CCTGATAGAC AATACAGGCG GGACGGCAGC CGCGGACGAA TGCTGGACAA CGAGCGCTGC 
CCCGACCCCC GTTACAGAAG TGAAAATACC TTGGATCGCG ACCGCAGTCC AGACCCCCGT 
TATCGAAGTG AACGCACGCT GGACCGCAAC TACAGCCCAG AGCGAGGTCG CCGCTTTCGC 
AGCGAACACA ACCTGGACCG AGAGCGAAGT CCAGACACAC AATACCGCAG CGATCGCACA 
CTGGACAGAA CACACAGTCC AGATACACAC TATAGGCCTG AACCTGCTCG GGGGTACAGC 
AGAGAGAATC TCACCAGTGA CAATGAGCGC AGGAGGTATG GCTCACATCA GAACGAACCC 
ATGAAGAAGA GCAACAGCCA AGACAGATTG GATCGTTCGC CTTCACCTGC ACCACTCCGA 
CACAATGCCT TGGAAAAGCC ACTCAGTGTT ACTCTCCTCA AAAACCGTCC CAATGATGAA 
TATGGCCTTC GTCTAGGCAG TCAGTTGTTC ATCAAAGAGA TGACGAACAC AGGCCTTGCA 
TCCAGGGAAG GCAAACTACA AGAAGGAGAC ATTATTCTGA AAATCAACGG TACGGTCACA 
GAGAATCTGT CTCTGAGCGA TGCAGGGAAG CTGATTGAGA AGTCTCGTGG AAAGCTGCAG 
CTGGTGGTGC AGAGGGATCG CAGTCAAGTG CTCATCCGCA TCCCACCAAT GGCAGACAGC 
GACTCGGAAA TGGATGACAT CTCTGAGATC GAGTCGTACC GCTCATATTC TCCTCAGGAG 
GATCGGCGGA GTCATCACTC AGACCTCTCC TCACACTCCT CCAATGAGAG ATTACAGGAC 
AAAAACAGAG AAGAGCCTCC TAAAAGACTG GCTAAAATGG GAGCGATGCC CACTCCATTT 
GTATCAGCAC CGGTGGATCT GCCACCTCCA CCTGTAGAGA AAGAAGAGCC TCGCAGCGAG 
TCTCCAGCTC CTCCTGTGGT AATTGCTGCT CCAAAAACTG TTCCAGTAAA AGCAAAACCT 
TTGCCAAAGG TTCCCTTGCT ACCCAGTCCA GAAGAGCAGG AAATTTATGG GCCCAACACT 
GTGATGGTGC GCTTTGTGAA GGGTGAAAGC GTTGGTCTGC GTCTGGCTGG AGGAAATGAT 
GTGGGCATTT TCATCGCCGG CGTTCAAGAG GACAGTCCTG CCGAGGTGGA GGGTCTGCGC 
ACTGGAGACC AAATAGTCAA GGTGAATAAC ATGGATTTTC GAGGCATGGT CCGTGAAGAT 
GCAGTTCTGT ATTTACTGGA GATTCCCAAA GGAGAGGATG TGACCATTTT GGCTCAAAGC 
AAACCCGATG TGTATAAAGA CATCCTGGGG TCTGGACGAG GTGATAACTT CTTCATCAGG 
ACTCATTTTG AATATGAGAA AGAGCTTCCC CAGAGCCTGA ACTTCTCTAG AGGAGAGGTT 
TTTAAAGTGG TGGACACCCT GTATGATGGC AAGCTGGGAA ACTGGCTCGC CATCCGCATG 
GACAAAGACA ATCAGCTCCT GGAAAAGGGC ATCATACCCA GCAAGAGCAG GGCGGAGCAG 
ATGGCAAATG TTCAGCATGC ACAGAAAGGT GCTGGTAATG ACAGAGGCGA TTTCTGGAGG 
CTCAGAGGTC AAAGGGCAGC TAAGAAAAAA GACCTTCGCA AGAGCAGAGA GGATCTCAGT 
GCAGCTCCAG TCACCACACG CTTCCCGGCC TATGAGAGAG TGGTGCTGCG TGAAGCTGGC 
TTCAGGCGTC CAGTCGTGAT TTTTGGCCCC ATTTCAGATG CAGCCAACGA AAAACTTGCC 
AATGAACTTC CAAATGAGTT CACCATTGCA AAGACCGAAC CAAAAGACGC AGGCTCAGAA 
AAGTCATCTG GCGTTGTGAG GCTAAACACC ATTCGTCAGA TCATCGAACA GGATCTACAT 
GCTCTGCTGG ATGTCACTCC AAAGGCTGTA GACACCCTCA ATTACACTCA GTGGTATCCC 
ATTGTCATCT TCCTCAACCC AGACAGCAAG CAGGGGGTGA AGACCATGAG GAATCGCTTG 
GTGCCTGGAT CCAACCGCAG CTCACGCAAA CTTTACGAAC AAGCGGTCAA ACTGCGGAAG 
ACATATGCGC ATCTGTTCAC AGCTACCATT GATCTGAACT CTTCTCACGA TGCCTGGTTT 
GGAAGCCTCA AAGACGCCGT ACGAGAACAG CAAGATAAAG CTGTCTGGGT CTGTCAAAGC 
AAGCTGGATG GCTCAGAGGA GGACCTGGAT CTCCATGATG ACCGTATGTC CTACCTGTCG 
GCCATGAGTG CAGATTACCT GAGCATGGAC AGCCGTCTGA CCAGCGACTA TGAGGACACG 
GCGGACGAGG GCGGCGCATA CACTGATAAT GAGCTTGATG AGTCCACAGA TGAGCCTCAG 
CCAATGTCTG CCATCAGCCG CTCATCTGAA CCCGTCAGTG AGGAGAGGCA CATGCCTGTT 
CCCCACGAGC GCTCAAAGAA ACCATCGGGC AGAGACGTCC AGCGTGACCC CAGTCCTCCT 
CCGTCATTTG TCCCAGAACC CCCAAAGGTG CGCTCTGCTT CCCGTCCTGC AGACTCCAGG 
AGTTTTGACT CGCGCTCCAG CAGCACCATC AGCAGTGATG CGCCCATCAG CAACAAACCT 
CTTCCTCCGC CCGTCGCCCA GAAGCCCAGT TTCAATACGA GGTCGGCGGA CGACACACCT 
TCTAATGACC CAGCAGATGA CCCTGCCAAC CGCACCTTCA GGGGAAAGGT GAAGGCCTTC 
GAGCAGATGG ACCACCTTGC CAGAGCCAAA CGTATGCTGG AGCTGCAGGA GGCCGAACAA 
GCCCGGCTGG AAATCTCTCA GAAACATCCA GATATTTATG CAGTTCCTAA CAAGCCAAAA 
CCAAACCAGA ACAGACCACA ACCAATTGGC TCCAGCTCAA ACTCCGAGTC CCAGAGCTCC 
TCCAAAGCAA TGTATTCAGA GAGAGGCTCA CACTACCGGC ACGATGATGA TGACGAAGAG 
GAGTATCGCC GCCAGCTGGC GGACCAGACC CGGAGAGGAT ATTACAGCAC ACAGAAATAC 
AATGACACAG AGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP2A ( XM_005155574.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155631.1 Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15901
Clone ID Related Accession (Same CDS sequence)	XM_005155574.4 , XM_005155574.3
Accession Version	XM_005155574.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155574.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGG TCCTCTCGCT GCACAGGAAA TGGGTGCACA CCATGAAGGC CATCCGCATC 
CTCCAGGGCA TCAACCCAGT CATGGAGGAG ACTGTGTGGG AGCAGTACAC TGTGACCCTA 
CAGCGGGACT CCAAGATGGG CTTTGGCCTA GCGGTGTCTG GAGGACGGGA CAATCCAAAC 
GAAGAAAGTG GAGAAATGTC AATCGTCATA TCTGATGTGC TGCAAGGGGG TCCAGCCGAT 
GGCTTGCTGT TTGAAAATGA CAGAGTGGTC CAGGTCAACA CTGTGCCGAT GGACGGTGTC 
CCACACTCCT TTGCAGTCCA GACTCTCCGC AAATGTGGCA AAGTTGCCAA AATTACTGTA 
AAGCGATGTA GAAAAATCCC TGTCAACCTG ACCAAGCGTG CTGCATCCCC TGATGACCGT 
GTCTTCAGTG GCGACTACAA CGATGATTAT GACTATGAAC AGGACCAGCA CAGCATCTAC 
AGTGGACGCA GCTACCCTAA CCGTGATACT AGTTTAGAGC GTGAGCGCGG CGGCTATGCT 
GACGAGCGTG AACGGGACTA TGATCGCAGT CGGGGGAGGA GCATGGAGCG CGGCGTGAGC 
CCTGATAGAC AATACAGGCG GGACGGCAGC CGCGGACGAA TGCTGGACAA CGAGCGCTGC 
CCCGACCCCC GTTACAGAAG TGAAAATACC TTGGATCGCG ACCGCAGTCC AGACCCCCGT 
TATCGAAGTG AACGCACGCT GGACCGCAAC TACAGCCCAG AGCGAGGTCG CCGCTTTCGC 
AGCGAACACA ACCTGGACCG AGAGCGAAGT CCAGACACAC AATACCGCAG CGATCGCACA 
CTGGACAGAA CACACAGTCC AGATACACAC TATAGGCCTG AACCTGCTCG GGGGTACAGC 
AGAGAGAATC TCACCAGTGA CAATGAGCGC AGGAGGTATG GCTCACATCA GAACGAACCC 
ATGAAGAAGA GCAACAGCCA AGACAGATTG GATCGTTCGC CTTCACCTGC ACCACTCCGA 
CACAATGCCT TGGAAAAGCC ACTCAGTGTT ACTCTCCTCA AAAACCGTCC CAATGATGAA 
TATGGCCTTC GTCTAGGCAG TCAGTTGTTC ATCAAAGAGA TGACGAACAC AGGCCTTGCA 
TCCAGGGAAG GCAAACTACA AGAAGGAGAC ATTATTCTGA AAATCAACGG TACGGTCACA 
GAGAATCTGT CTCTGAGCGA TGCAGGGAAG CTGATTGAGA AGTCTCGTGG AAAGCTGCAG 
CTGGTGGTGC AGAGGGATCG CAGTCAAGTG CTCATCCGCA TCCCACCAAT GGCAGACAGC 
GACTCGGAAA TGGATGACAT CTCTGAGATC GAGTCGTACC GCTCATATTC TCCTCAGGAG 
GATCGGCGGA GTCATCACTC AGACCTCTCC TCACACTCCT CCAATGAGAG ATTACAGGAC 
AAAAACAGAG AAGAGCCTCC TAAAAGACTG GCTAAAATGG GAGCGATGCC CACTCCATTT 
GTATCAGCAC CGGTGGATCT GCCACCTCCA CCTGTAGAGA AAGAAGAGCC TCGCAGCGAG 
TCTCCAGCTC CTCCTGTGGT AATTGCTGCT CCAAAAACTG TTCCAGTAAA AGCAAAACCT 
TTGCCAAAGG TTCCCTTGCT ACCCAGTCCA GAAGAGCAGG AAATTTATGG GCCCAACACT 
GTGATGGTGC GCTTTGTGAA GGGTGAAAGC GTTGGTCTGC GTCTGGCTGG AGGAAATGAT 
GTGGGCATTT TCATCGCCGG CGTTCAAGAG GACAGTCCTG CCGAGGTGGA GGGTCTGCGC 
ACTGGAGACC AAATAGTCAA GGTGAATAAC ATGGATTTTC GAGGCATGGT CCGTGAAGAT 
GCAGTTCTGT ATTTACTGGA GATTCCCAAA GGAGAGGATG TGACCATTTT GGCTCAAAGC 
AAACCCGATG TGTATAAAGA CATCCTGGGG TCTGGACGAG GTGATAACTT CTTCATCAGG 
ACTCATTTTG AATATGAGAA AGAGCTTCCC CAGAGCCTGA ACTTCTCTAG AGGAGAGGTT 
TTTAAAGTGG TGGACACCCT GTATGATGGC AAGCTGGGAA ACTGGCTCGC CATCCGCATG 
GACAAAGACA ATCAGCTCCT GGAAAAGGGC ATCATACCCA GCAAGAGCAG GGCGGAGCAG 
ATGGCAAATG TTCAGCATGC ACAGAAAGGT GCTGGTAATG ACAGAGGCGA TTTCTGGAGG 
CTCAGAGGTC AAAGGGCAGC TAAGAAAAAA GACCTTCGCA AGAGCAGAGA GGATCTCAGT 
GCAGCTCCAG TCACCACACG CTTCCCGGCC TATGAGAGAG TGGTGCTGCG TGAAGCTGGC 
TTCAGGCGTC CAGTCGTGAT TTTTGGCCCC ATTTCAGATG CAGCCAACGA AAAACTTGCC 
AATGAACTTC CAAATGAGTT CACCATTGCA AAGACCGAAC CAAAAGACGC AGGCTCAGAA 
AAGTCATCTG GCGTTGTGAG GCTAAACACC ATTCGTCAGA TCATCGAACA GGATCTACAT 
GCTCTGCTGG ATGTCACTCC AAAGGCTGTA GACACCCTCA ATTACACTCA GTGGTATCCC 
ATTGTCATCT TCCTCAACCC AGACAGCAAG CAGGGGGTGA AGACCATGAG GAATCGCTTG 
GTGCCTGGAT CCAACCGCAG CTCACGCAAA CTTTACGAAC AAGCGGTCAA ACTGCGGAAG 
ACATATGCGC ATCTGTTCAC AGCTACCATT GATCTGAACT CTTCTCACGA TGCCTGGTTT 
GGAAGCCTCA AAGACGCCGT ACGAGAACAG CAAGATAAAG CTGTCTGGGT CTGTCAAAGC 
AAGCTGGATG GCTCAGAGGA GGACCTGGAT CTCCATGATG ACCGTATGTC CTACCTGTCG 
GCCATGAGTG CAGATTACCT GAGCATGGAC AGCCGTCTGA CCAGCGACTA TGAGGACACG 
GCGGACGAGG GCGGCGCATA CACTGATAAT GAGCTTGATG AGTCCACAGA TGAGCCTCAG 
CCAATGTCTG CCATCAGCCG CTCATCTGAA CCCGTCAGTG AGGAGAGGCA CATGCCTGTT 
CCCCACGAGC GCTCAAAGAA ACCATCGGGC AGAGACGTCC AGCGTGACCC CAGTCCTCCT 
CCGTCATTTG TCCCAGAACC CCCAAAGGTG CGCTCTGCTT CCCGTCCTGC AGACTCCAGG 
AGTTTTGACT CGCGCTCCAG CAGCACCATC AGCAGTGATG CGCCCATCAG CAACAAACCT 
CTTCCTCCGC CCGTCGCCCA GAAGCCCAGT TTCAATACGA GGTCGGCGGA CGACACACCT 
TCTAATGACC CAGCAGATGA CCCTGCCAAC CGCACCTTCA GGGGAAAGGT GAAGGCCTTC 
GAGCAGATGG ACCACCTTGC CAGAGCCAAA CGTATGCTGG AGCTGCAGGA GGCCGAACAA 
GCCCGGCTGG AAATCTCTCA GAAACATCCA GATATTTATG CAGTTCCTAA CAAGCCAAAA 
CCAAACCAGA ACAGACCACA ACCAATTGGC TCCAGCTCAA ACTCCGAGTC CCAGAGCTCC 
TCCAAAGCAA TGTATTCAGA GAGAGGCTCA CACTACCGGC ACGATGATGA TGACGAAGAG 
GAGTATCGCC GCCAGCTGGC GGACCAGACC CGGAGAGGAT ATTACAGCAC ACAGAAATAC 
AATGACACAG AGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155631.1
CDS	320..3997
Translation	MKTVLSLHRKWVHTMKAIRILQGINPVMEETVWEQYTVTLQRDSKMGFGLAVSGGRDNPNEESGEMSIVISDVLQGGPADGLLFENDRVVQVNTVPMDGVPHSFAVQTLRKCGKVAKITVKRCRKIPVNLTKRAASPDDRVFSGDYNDDYDYEQDQHSIYSGRSYPNRDTSLERERGGYADERERDYDRSRGRSMERGVSPDRQYRRDGSRGRMLDNERCPDPRYRSENTLDRDRSPDPRYRSERTLDRNYSPERGRRFRSEHNLDRERSPDTQYRSDRTLDRTHSPDTHYRPEPARGYSRENLTSDNERRRYGSHQNEPMKKSNSQDRLDRSPSPAPLRHNALEKPLSVTLLKNRPNDEYGLRLGSQLFIKEMTNTGLASREGKLQEGDIILKINGTVTENLSLSDAGKLIEKSRGKLQLVVQRDRSQVLIRIPPMADSDSEMDDISEIESYRSYSPQEDRRSHHSDLSSHSSNERLQDKNREEPPKRLAKMGAMPTPFVSAPVDLPPPPVEKEEPRSESPAPPVVIAAPKTVPVKAKPLPKVPLLPSPEEQEIYGPNTVMVRFVKGESVGLRLAGGNDVGIFIAGVQEDSPAEVEGLRTGDQIVKVNNMDFRGMVREDAVLYLLEIPKGEDVTILAQSKPDVYKDILGSGRGDNFFIRTHFEYEKELPQSLNFSRGEVFKVVDTLYDGKLGNWLAIRMDKDNQLLEKGIIPSKSRAEQMANVQHAQKGAGNDRGDFWRLRGQRAAKKKDLRKSREDLSAAPVTTRFPAYERVVLREAGFRRPVVIFGPISDAANEKLANELPNEFTIAKTEPKDAGSEKSSGVVRLNTIRQIIEQDLHALLDVTPKAVDTLNYTQWYPIVIFLNPDSKQGVKTMRNRLVPGSNRSSRKLYEQAVKLRKTYAHLFTATIDLNSSHDAWFGSLKDAVREQQDKAVWVCQSKLDGSEEDLDLHDDRMSYLSAMSADYLSMDSRLTSDYEDTADEGGAYTDNELDESTDEPQPMSAISRSSEPVSEERHMPVPHERSKKPSGRDVQRDPSPPPSFVPEPPKVRSASRPADSRSFDSRSSSTISSDAPISNKPLPPPVAQKPSFNTRSADDTPSNDPADDPANRTFRGKVKAFEQMDHLARAKRMLELQEAEQARLEISQKHPDIYAVPNKPKPNQNRPQPIGSSSNSESQSSSKAMYSERGSHYRHDDDDEEEYRRQLADQTRRGYYSTQKYNDTEL

Target ORF information:

RefSeq Version	XM_005155574.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155574.3

ORF Insert Sequence:

ATGAAGACGG TCCTCTCGCT GCACAGGAAA TGGGTGCACA CCATGAAGGC CATCCGCATC 
CTCCAGGGCA TCAACCCAGT CATGGAGGAG ACTGTGTGGG AGCAGTACAC TGTGACCCTA 
CAGCGGGACT CCAAGATGGG CTTTGGCCTA GCGGTGTCTG GAGGACGGGA CAATCCAAAC 
GAAGAAAGTG GAGAAATGTC AATCGTCATA TCTGATGTGC TGCAAGGGGG TCCAGCCGAT 
GGCTTGCTGT TTGAAAATGA CAGAGTGGTC CAGGTCAACA CTGTGCCGAT GGACGGTGTC 
CCACACTCCT TTGCAGTCCA GACTCTCCGC AAATGTGGCA AAGTTGCCAA AATTACTGTA 
AAGCGATGTA GAAAAATCCC TGTCAACCTG ACCAAGCGTG CTGCATCCCC TGATGACCGT 
GTCTTCAGTG GCGACTACAA CGATGATTAT GACTATGAAC AGGACCAGCA CAGCATCTAC 
AGTGGACGCA GCTACCCTAA CCGTGATACT AGTTTAGAGC GTGAGCGCGG CGGCTATGCT 
GACGAGCGTG AACGGGACTA TGATCGCAGT CGGGGGAGGA GCATGGAGCG CGGCGTGAGC 
CCTGATAGAC AATACAGGCG GGACGGCAGC CGCGGACGAA TGCTGGACAA CGAGCGCTGC 
CCCGACCCCC GTTACAGAAG TGAAAATACC TTGGATCGCG ACCGCAGTCC AGACCCCCGT 
TATCGAAGTG AACGCACGCT GGACCGCAAC TACAGCCCAG AGCGAGGTCG CCGCTTTCGC 
AGCGAACACA ACCTGGACCG AGAGCGAAGT CCAGACACAC AATACCGCAG CGATCGCACA 
CTGGACAGAA CACACAGTCC AGATACACAC TATAGGCCTG AACCTGCTCG GGGGTACAGC 
AGAGAGAATC TCACCAGTGA CAATGAGCGC AGGAGGTATG GCTCACATCA GAACGAACCC 
ATGAAGAAGA GCAACAGCCA AGACAGATTG GATCGTTCGC CTTCACCTGC ACCACTCCGA 
CACAATGCCT TGGAAAAGCC ACTCAGTGTT ACTCTCCTCA AAAACCGTCC CAATGATGAA 
TATGGCCTTC GTCTAGGCAG TCAGTTGTTC ATCAAAGAGA TGACGAACAC AGGCCTTGCA 
TCCAGGGAAG GCAAACTACA AGAAGGAGAC ATTATTCTGA AAATCAACGG TACGGTCACA 
GAGAATCTGT CTCTGAGCGA TGCAGGGAAG CTGATTGAGA AGTCTCGTGG AAAGCTGCAG 
CTGGTGGTGC AGAGGGATCG CAGTCAAGTG CTCATCCGCA TCCCACCAAT GGCAGACAGC 
GACTCGGAAA TGGATGACAT CTCTGAGATC GAGTCGTACC GCTCATATTC TCCTCAGGAG 
GATCGGCGGA GTCATCACTC AGACCTCTCC TCACACTCCT CCAATGAGAG ATTACAGGAC 
AAAAACAGAG AAGAGCCTCC TAAAAGACTG GCTAAAATGG GAGCGATGCC CACTCCATTT 
GTATCAGCAC CGGTGGATCT GCCACCTCCA CCTGTAGAGA AAGAAGAGCC TCGCAGCGAG 
TCTCCAGCTC CTCCTGTGGT AATTGCTGCT CCAAAAACTG TTCCAGTAAA AGCAAAACCT 
TTGCCAAAGG TTCCCTTGCT ACCCAGTCCA GAAGAGCAGG AAATTTATGG GCCCAACACT 
GTGATGGTGC GCTTTGTGAA GGGTGAAAGC GTTGGTCTGC GTCTGGCTGG AGGAAATGAT 
GTGGGCATTT TCATCGCCGG CGTTCAAGAG GACAGTCCTG CCGAGGTGGA GGGTCTGCGC 
ACTGGAGACC AAATAGTCAA GGTGAATAAC ATGGATTTTC GAGGCATGGT CCGTGAAGAT 
GCAGTTCTGT ATTTACTGGA GATTCCCAAA GGAGAGGATG TGACCATTTT GGCTCAAAGC 
AAACCCGATG TGTATAAAGA CATCCTGGGG TCTGGACGAG GTGATAACTT CTTCATCAGG 
ACTCATTTTG AATATGAGAA AGAGCTTCCC CAGAGCCTGA ACTTCTCTAG AGGAGAGGTT 
TTTAAAGTGG TGGACACCCT GTATGATGGC AAGCTGGGAA ACTGGCTCGC CATCCGCATG 
GACAAAGACA ATCAGCTCCT GGAAAAGGGC ATCATACCCA GCAAGAGCAG GGCGGAGCAG 
ATGGCAAATG TTCAGCATGC ACAGAAAGGT GCTGGTAATG ACAGAGGCGA TTTCTGGAGG 
CTCAGAGGTC AAAGGGCAGC TAAGAAAAAA GACCTTCGCA AGAGCAGAGA GGATCTCAGT 
GCAGCTCCAG TCACCACACG CTTCCCGGCC TATGAGAGAG TGGTGCTGCG TGAAGCTGGC 
TTCAGGCGTC CAGTCGTGAT TTTTGGCCCC ATTTCAGATG CAGCCAACGA AAAACTTGCC 
AATGAACTTC CAAATGAGTT CACCATTGCA AAGACCGAAC CAAAAGACGC AGGCTCAGAA 
AAGTCATCTG GCGTTGTGAG GCTAAACACC ATTCGTCAGA TCATCGAACA GGATCTACAT 
GCTCTGCTGG ATGTCACTCC AAAGGCTGTA GACACCCTCA ATTACACTCA GTGGTATCCC 
ATTGTCATCT TCCTCAACCC AGACAGCAAG CAGGGGGTGA AGACCATGAG GAATCGCTTG 
GTGCCTGGAT CCAACCGCAG CTCACGCAAA CTTTACGAAC AAGCGGTCAA ACTGCGGAAG 
ACATATGCGC ATCTGTTCAC AGCTACCATT GATCTGAACT CTTCTCACGA TGCCTGGTTT 
GGAAGCCTCA AAGACGCCGT ACGAGAACAG CAAGATAAAG CTGTCTGGGT CTGTCAAAGC 
AAGCTGGATG GCTCAGAGGA GGACCTGGAT CTCCATGATG ACCGTATGTC CTACCTGTCG 
GCCATGAGTG CAGATTACCT GAGCATGGAC AGCCGTCTGA CCAGCGACTA TGAGGACACG 
GCGGACGAGG GCGGCGCATA CACTGATAAT GAGCTTGATG AGTCCACAGA TGAGCCTCAG 
CCAATGTCTG CCATCAGCCG CTCATCTGAA CCCGTCAGTG AGGAGAGGCA CATGCCTGTT 
CCCCACGAGC GCTCAAAGAA ACCATCGGGC AGAGACGTCC AGCGTGACCC CAGTCCTCCT 
CCGTCATTTG TCCCAGAACC CCCAAAGGTG CGCTCTGCTT CCCGTCCTGC AGACTCCAGG 
AGTTTTGACT CGCGCTCCAG CAGCACCATC AGCAGTGATG CGCCCATCAG CAACAAACCT 
CTTCCTCCGC CCGTCGCCCA GAAGCCCAGT TTCAATACGA GGTCGGCGGA CGACACACCT 
TCTAATGACC CAGCAGATGA CCCTGCCAAC CGCACCTTCA GGGGAAAGGT GAAGGCCTTC 
GAGCAGATGG ACCACCTTGC CAGAGCCAAA CGTATGCTGG AGCTGCAGGA GGCCGAACAA 
GCCCGGCTGG AAATCTCTCA GAAACATCCA GATATTTATG CAGTTCCTAA CAAGCCAAAA 
CCAAACCAGA ACAGACCACA ACCAATTGGC TCCAGCTCAA ACTCCGAGTC CCAGAGCTCC 
TCCAAAGCAA TGTATTCAGA GAGAGGCTCA CACTACCGGC ACGATGATGA TGACGAAGAG 
GAGTATCGCC GCCAGCTGGC GGACCAGACC CGGAGAGGAT ATTACAGCAC ACAGAAATAC 
AATGACACAG AGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP2A ( XM_009305192.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303467.1 Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44613
Clone ID Related Accession (Same CDS sequence)	XM_009305192.2 , XM_009305192.3
Accession Version	XM_009305192.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA AGAAATTCAT CACCATCATG CAAGCGGCCA TCGGGGTCGC ACCGCTCAAC 
AAACGAGAGC TGATGCCACC CGGCCGGAGG TTGTGGAGAC CCTCAGGTGA CCAGACCAAC 
CAAATTGAGT TGTTCCGCTC AAGCCGTTCC AACAAGAAAA GGTTTTCCTT GACCTACGAA 
GCTCAATATC GGTGTCTGCG ACGCCTCTCT ACTCCAAGCT TCCCGGCCCT TTTACAGAAC 
CCAGTCATGG AGGAGACTGT GTGGGAGCAG TACACTGTGA CCCTACAGCG GGACTCCAAG 
ATGGGCTTTG GCCTAGCGGT GTCTGGAGGA CGGGACAATC CAAACGAAGA AAGTGGAGAA 
ATGTCAATCG TCATATCTGA TGTGCTGCAA GGGGGTCCAG CCGATGGCTT GCTGTTTGAA 
AATGACAGAG TGGTCCAGGT CAACACTGTG CCGATGGACG GTGTCCCACA CTCCTTTGCA 
GTCCAGACTC TCCGCAAATG TGGCAAAGTT GCCAAAATTA CTGTAAAGCG ATGTAGAAAA 
ATCCCTGTCA ACCTGACCAA GCGTGCTGCA TCCCCTGATG ACCGTGTCTT CAGTGGCGAC 
TACAACGATG ATTATGACTA TGAACAGGAC CAGCACAGCA TCTACAGTGG ACGCAGCTAC 
CCTAACCGTG ATACTAGTTT AGAGCGTGAG CGCGGCGGCT ATGCTGACGA GCGTGAACGG 
GACTATGATC GCAGTCGGGG GAGGAGCATG GAGCGCGGCG TGAGCCCTGA TAGACAATAC 
AGGCGGGACG GCAGCCGCGG ACGAATGCTG GACAACGAGC GCTGCCCCGA CCCCCGTTAC 
AGAAGTGAAA ATACCTTGGA TCGCGACCGC AGTCCAGACC CCCGTTATCG AAGTGAACGC 
ACGCTGGACC GCAACTACAG CCCAGAGCGA GGTCGCCGCT TTCGCAGCGA ACACAACCTG 
GACCGAGAGC GAAGTCCAGA CACACAATAC CGCAGCGATC GCACACTGGA CAGAACACAC 
AGTCCAGATA CACACTATAG GCCTGAACCT GCTCGGGGGT ACAGCAGAGA GAATCTCACC 
AGTGACAATG AGCGCAGGAG GTATGGCTCA CATCAGAACG AACCCATGAA GAAGAGCAAC 
AGCCAAGACA GATTGGATCG TTCGCCTTCA CCTGCACCAC TCCGACACAA TGCCTTGGAA 
AAGCCACTCA GTGTTACTCT CCTCAAAAAC CGTCCCAATG ATGAATATGG CCTTCGTCTA 
GGCAGTCAGT TGTTCATCAA AGAGATGACG AACACAGGCC TTGCATCCAG GGAAGGCAAA 
CTACAAGAAG GAGACATTAT TCTGAAAATC AACGGTACGG TCACAGAGAA TCTGTCTCTG 
AGCGATGCAG GGAAGCTGAT TGAGAAGTCT CGTGGAAAGC TGCAGCTGGT GGTGCAGAGG 
GATCGCAGTC AAGTGCTCAT CCGCATCCCA CCAATGGCAG ACAGCGACTC GGAAATGGAT 
GACATCTCTG AGATCGAGTC GTACCGCTCA TATTCTCCTC AGGAGGATCG GCGGAGTCAT 
CACTCAGACC TCTCCTCACA CTCCTCCAAT GAGAGATTAC AGGACAAAAA CAGAGAAGAG 
CCTCCTAAAA GACTGGCTAA AATGGGAGCG ATGCCCACTC CATTTGTATC AGCACCGGTG 
GATCTGCCAC CTCCACCTGT AGAGAAAGAA GAGCCTCGCA GCGAGTCTCC AGCTCCTCCT 
GTGGTAATTG CTGCTCCAAA AACTGTTCCA GTAAAAGCAA AACCTTTGCC AAAGGTTCCC 
TTGCTACCCA GTCCAGAAGA GCAGGAAATT TATGGGCCCA ACACTGTGAT GGTGCGCTTT 
GTGAAGGGTG AAAGCGTTGG TCTGCGTCTG GCTGGAGGAA ATGATGTGGG CATTTTCATC 
GCCGGCGTTC AAGAGGACAG TCCTGCCGAG GTGGAGGGTC TGCGCACTGG AGACCAAATA 
GTCAAGGTGA ATAACATGGA TTTTCGAGGC ATGGTCCGTG AAGATGCAGT TCTGTATTTA 
CTGGAGATTC CCAAAGGAGA GGATGTGACC ATTTTGGCTC AAAGCAAACC CGATGTGTAT 
AAAGACATCC TGGGGTCTGG ACGAGGTGAT AACTTCTTCA TCAGGACTCA TTTTGAATAT 
GAGAAAGAGC TTCCCCAGAG CCTGAACTTC TCTAGAGGAG AGGTTTTTAA AGTGGTGGAC 
ACCCTGTATG ATGGCAAGCT GGGAAACTGG CTCGCCATCC GCATGGACAA AGACAATCAG 
CTCCTGGAAA AGGGCATCAT ACCCAGCAAG AGCAGGGCGG AGCAGATGGC AAATGTTCAG 
CATGCACAGA AAGGTGCTGG TAATGACAGA GGCGATTTCT GGAGGCTCAG AGGTCAAAGG 
GCAGCTAAGA AAAAAGACCT TCGCAAGAGC AGAGAGGATC TCAGTGCAGC TCCAGTCACC 
ACACGCTTCC CGGCCTATGA GAGAGTGGTG CTGCGTGAAG CTGGCTTCAG GCGTCCAGTC 
GTGATTTTTG GCCCCATTTC AGATGCAGCC AACGAAAAAC TTGCCAATGA ACTTCCAAAT 
GAGTTCACCA TTGCAAAGAC CGAACCAAAA GACGCAGGCT CAGAAAAGTC ATCTGGCGTT 
GTGAGGCTAA ACACCATTCG TCAGATCATC GAACAGGATC TACATGCTCT GCTGGATGTC 
ACTCCAAAGG CTGTAGACAC CCTCAATTAC ACTCAGTGGT ATCCCATTGT CATCTTCCTC 
AACCCAGACA GCAAGCAGGG GGTGAAGACC ATGAGGAATC GCTTGGTGCC TGGATCCAAC 
CGCAGCTCAC GCAAACTTTA CGAACAAGCG GTCAAACTGC GGAAGACATA TGCGCATCTG 
TTCACAGCTA CCATTGATCT GAACTCTTCT CACGATGCCT GGTTTGGAAG CCTCAAAGAC 
GCCGTACGAG AACAGCAAGA TAAAGCTGTC TGGGTCTGTC AAAGCAAGCT GGATGGCTCA 
GAGGAGGACC TGGATCTCCA TGATGACCGT ATGTCCTACC TGTCGGCCAT GAGTGCAGAT 
TACCTGAGCA TGGACAGCCG TCTGACCAGC GACTATGAGG ACACGGCGGA CGAGGGCGGC 
GCATACACTG ATAATGAGCT TGATGAGTCC ACAGATGAGC CTCAGCCAAT GTCTGCCATC 
AGCCGCTCAT CTGAACCCGT CAGTGAGGAG AGGCACATGC CTGTTCCCCA CGAGCGCTCA 
AAGAAACCAT CGGGCAGAGA CGTCCAGCGT GACCCCAGTC CTCCTCCGTC ATTTGTCCCA 
GAACCCCCAA AGGTGCGCTC TGCTTCCCGT CCTGCAGACT CCAGGAGTTT TGACTCGCGC 
TCCAGCAGCA CCATCAGCAG TGATGCGCCC ATCAGCAACA AACCTCTTCC TCCGCCCGTC 
GCCCAGAAGC CCAGTTTCAA TACGAGGTCG GCGGACGACA CACCTTCTAA TGACCCAGCA 
GATGACCCTG CCAACCGCAC CTTCAGGGGA AAGGTGAAGG CCTTCGAGCA GATGGACCAC 
CTTGCCAGAG CCAAACGTAT GCTGGAGCTG CAGGAGGCCG AACAAGCCCG GCTGGAAATC 
TCTCAGAAAC ATCCAGATAT TTATGCAGTT CCTAACAAGC CAAAACCAAA CCAGAACAGA 
CCACAACCAA TTGGCTCCAG CTCAAACTCC GAGTCCCAGA GCTCCTCCAA AGCAATGTAT 
TCAGAGAGAG GCTCACACTA CCGGCACGAT GATGATGACG AAGAGGAGTA TCGCCGCCAG 
CTGGCGGACC AGACCCGGAG AGGATATTAC AGCACACAGA AATACAATGA CACAGAGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303467.1
CDS	266..4108
Translation	MKYKKFITIMQAAIGVAPLNKRELMPPGRRLWRPSGDQTNQIELFRSSRSNKKRFSLTYEAQYRCLRRLSTPSFPALLQNPVMEETVWEQYTVTLQRDSKMGFGLAVSGGRDNPNEESGEMSIVISDVLQGGPADGLLFENDRVVQVNTVPMDGVPHSFAVQTLRKCGKVAKITVKRCRKIPVNLTKRAASPDDRVFSGDYNDDYDYEQDQHSIYSGRSYPNRDTSLERERGGYADERERDYDRSRGRSMERGVSPDRQYRRDGSRGRMLDNERCPDPRYRSENTLDRDRSPDPRYRSERTLDRNYSPERGRRFRSEHNLDRERSPDTQYRSDRTLDRTHSPDTHYRPEPARGYSRENLTSDNERRRYGSHQNEPMKKSNSQDRLDRSPSPAPLRHNALEKPLSVTLLKNRPNDEYGLRLGSQLFIKEMTNTGLASREGKLQEGDIILKINGTVTENLSLSDAGKLIEKSRGKLQLVVQRDRSQVLIRIPPMADSDSEMDDISEIESYRSYSPQEDRRSHHSDLSSHSSNERLQDKNREEPPKRLAKMGAMPTPFVSAPVDLPPPPVEKEEPRSESPAPPVVIAAPKTVPVKAKPLPKVPLLPSPEEQEIYGPNTVMVRFVKGESVGLRLAGGNDVGIFIAGVQEDSPAEVEGLRTGDQIVKVNNMDFRGMVREDAVLYLLEIPKGEDVTILAQSKPDVYKDILGSGRGDNFFIRTHFEYEKELPQSLNFSRGEVFKVVDTLYDGKLGNWLAIRMDKDNQLLEKGIIPSKSRAEQMANVQHAQKGAGNDRGDFWRLRGQRAAKKKDLRKSREDLSAAPVTTRFPAYERVVLREAGFRRPVVIFGPISDAANEKLANELPNEFTIAKTEPKDAGSEKSSGVVRLNTIRQIIEQDLHALLDVTPKAVDTLNYTQWYPIVIFLNPDSKQGVKTMRNRLVPGSNRSSRKLYEQAVKLRKTYAHLFTATIDLNSSHDAWFGSLKDAVREQQDKAVWVCQSKLDGSEEDLDLHDDRMSYLSAMSADYLSMDSRLTSDYEDTADEGGAYTDNELDESTDEPQPMSAISRSSEPVSEERHMPVPHERSKKPSGRDVQRDPSPPPSFVPEPPKVRSASRPADSRSFDSRSSSTISSDAPISNKPLPPPVAQKPSFNTRSADDTPSNDPADDPANRTFRGKVKAFEQMDHLARAKRMLELQEAEQARLEISQKHPDIYAVPNKPKPNQNRPQPIGSSSNSESQSSSKAMYSERGSHYRHDDDDEEEYRRQLADQTRRGYYSTQKYNDTEL

Target ORF information:

RefSeq Version	XM_009305192.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305192.2

ORF Insert Sequence:

ATGAAGTACA AGAAATTCAT CACCATCATG CAAGCGGCCA TCGGGGTCGC ACCGCTCAAC 
AAACGAGAGC TGATGCCACC CGGCCGGAGG TTGTGGAGAC CCTCAGGTGA CCAGACCAAC 
CAAATTGAGT TGTTCCGCTC AAGCCGTTCC AACAAGAAAA GGTTTTCCTT GACCTACGAA 
GCTCAATATC GGTGTCTGCG ACGCCTCTCT ACTCCAAGCT TCCCGGCCCT TTTACAGAAC 
CCAGTCATGG AGGAGACTGT GTGGGAGCAG TACACTGTGA CCCTACAGCG GGACTCCAAG 
ATGGGCTTTG GCCTAGCGGT GTCTGGAGGA CGGGACAATC CAAACGAAGA AAGTGGAGAA 
ATGTCAATCG TCATATCTGA TGTGCTGCAA GGGGGTCCAG CCGATGGCTT GCTGTTTGAA 
AATGACAGAG TGGTCCAGGT CAACACTGTG CCGATGGACG GTGTCCCACA CTCCTTTGCA 
GTCCAGACTC TCCGCAAATG TGGCAAAGTT GCCAAAATTA CTGTAAAGCG ATGTAGAAAA 
ATCCCTGTCA ACCTGACCAA GCGTGCTGCA TCCCCTGATG ACCGTGTCTT CAGTGGCGAC 
TACAACGATG ATTATGACTA TGAACAGGAC CAGCACAGCA TCTACAGTGG ACGCAGCTAC 
CCTAACCGTG ATACTAGTTT AGAGCGTGAG CGCGGCGGCT ATGCTGACGA GCGTGAACGG 
GACTATGATC GCAGTCGGGG GAGGAGCATG GAGCGCGGCG TGAGCCCTGA TAGACAATAC 
AGGCGGGACG GCAGCCGCGG ACGAATGCTG GACAACGAGC GCTGCCCCGA CCCCCGTTAC 
AGAAGTGAAA ATACCTTGGA TCGCGACCGC AGTCCAGACC CCCGTTATCG AAGTGAACGC 
ACGCTGGACC GCAACTACAG CCCAGAGCGA GGTCGCCGCT TTCGCAGCGA ACACAACCTG 
GACCGAGAGC GAAGTCCAGA CACACAATAC CGCAGCGATC GCACACTGGA CAGAACACAC 
AGTCCAGATA CACACTATAG GCCTGAACCT GCTCGGGGGT ACAGCAGAGA GAATCTCACC 
AGTGACAATG AGCGCAGGAG GTATGGCTCA CATCAGAACG AACCCATGAA GAAGAGCAAC 
AGCCAAGACA GATTGGATCG TTCGCCTTCA CCTGCACCAC TCCGACACAA TGCCTTGGAA 
AAGCCACTCA GTGTTACTCT CCTCAAAAAC CGTCCCAATG ATGAATATGG CCTTCGTCTA 
GGCAGTCAGT TGTTCATCAA AGAGATGACG AACACAGGCC TTGCATCCAG GGAAGGCAAA 
CTACAAGAAG GAGACATTAT TCTGAAAATC AACGGTACGG TCACAGAGAA TCTGTCTCTG 
AGCGATGCAG GGAAGCTGAT TGAGAAGTCT CGTGGAAAGC TGCAGCTGGT GGTGCAGAGG 
GATCGCAGTC AAGTGCTCAT CCGCATCCCA CCAATGGCAG ACAGCGACTC GGAAATGGAT 
GACATCTCTG AGATCGAGTC GTACCGCTCA TATTCTCCTC AGGAGGATCG GCGGAGTCAT 
CACTCAGACC TCTCCTCACA CTCCTCCAAT GAGAGATTAC AGGACAAAAA CAGAGAAGAG 
CCTCCTAAAA GACTGGCTAA AATGGGAGCG ATGCCCACTC CATTTGTATC AGCACCGGTG 
GATCTGCCAC CTCCACCTGT AGAGAAAGAA GAGCCTCGCA GCGAGTCTCC AGCTCCTCCT 
GTGGTAATTG CTGCTCCAAA AACTGTTCCA GTAAAAGCAA AACCTTTGCC AAAGGTTCCC 
TTGCTACCCA GTCCAGAAGA GCAGGAAATT TATGGGCCCA ACACTGTGAT GGTGCGCTTT 
GTGAAGGGTG AAAGCGTTGG TCTGCGTCTG GCTGGAGGAA ATGATGTGGG CATTTTCATC 
GCCGGCGTTC AAGAGGACAG TCCTGCCGAG GTGGAGGGTC TGCGCACTGG AGACCAAATA 
GTCAAGGTGA ATAACATGGA TTTTCGAGGC ATGGTCCGTG AAGATGCAGT TCTGTATTTA 
CTGGAGATTC CCAAAGGAGA GGATGTGACC ATTTTGGCTC AAAGCAAACC CGATGTGTAT 
AAAGACATCC TGGGGTCTGG ACGAGGTGAT AACTTCTTCA TCAGGACTCA TTTTGAATAT 
GAGAAAGAGC TTCCCCAGAG CCTGAACTTC TCTAGAGGAG AGGTTTTTAA AGTGGTGGAC 
ACCCTGTATG ATGGCAAGCT GGGAAACTGG CTCGCCATCC GCATGGACAA AGACAATCAG 
CTCCTGGAAA AGGGCATCAT ACCCAGCAAG AGCAGGGCGG AGCAGATGGC AAATGTTCAG 
CATGCACAGA AAGGTGCTGG TAATGACAGA GGCGATTTCT GGAGGCTCAG AGGTCAAAGG 
GCAGCTAAGA AAAAAGACCT TCGCAAGAGC AGAGAGGATC TCAGTGCAGC TCCAGTCACC 
ACACGCTTCC CGGCCTATGA GAGAGTGGTG CTGCGTGAAG CTGGCTTCAG GCGTCCAGTC 
GTGATTTTTG GCCCCATTTC AGATGCAGCC AACGAAAAAC TTGCCAATGA ACTTCCAAAT 
GAGTTCACCA TTGCAAAGAC CGAACCAAAA GACGCAGGCT CAGAAAAGTC ATCTGGCGTT 
GTGAGGCTAA ACACCATTCG TCAGATCATC GAACAGGATC TACATGCTCT GCTGGATGTC 
ACTCCAAAGG CTGTAGACAC CCTCAATTAC ACTCAGTGGT ATCCCATTGT CATCTTCCTC 
AACCCAGACA GCAAGCAGGG GGTGAAGACC ATGAGGAATC GCTTGGTGCC TGGATCCAAC 
CGCAGCTCAC GCAAACTTTA CGAACAAGCG GTCAAACTGC GGAAGACATA TGCGCATCTG 
TTCACAGCTA CCATTGATCT GAACTCTTCT CACGATGCCT GGTTTGGAAG CCTCAAAGAC 
GCCGTACGAG AACAGCAAGA TAAAGCTGTC TGGGTCTGTC AAAGCAAGCT GGATGGCTCA 
GAGGAGGACC TGGATCTCCA TGATGACCGT ATGTCCTACC TGTCGGCCAT GAGTGCAGAT 
TACCTGAGCA TGGACAGCCG TCTGACCAGC GACTATGAGG ACACGGCGGA CGAGGGCGGC 
GCATACACTG ATAATGAGCT TGATGAGTCC ACAGATGAGC CTCAGCCAAT GTCTGCCATC 
AGCCGCTCAT CTGAACCCGT CAGTGAGGAG AGGCACATGC CTGTTCCCCA CGAGCGCTCA 
AAGAAACCAT CGGGCAGAGA CGTCCAGCGT GACCCCAGTC CTCCTCCGTC ATTTGTCCCA 
GAACCCCCAA AGGTGCGCTC TGCTTCCCGT CCTGCAGACT CCAGGAGTTT TGACTCGCGC 
TCCAGCAGCA CCATCAGCAG TGATGCGCCC ATCAGCAACA AACCTCTTCC TCCGCCCGTC 
GCCCAGAAGC CCAGTTTCAA TACGAGGTCG GCGGACGACA CACCTTCTAA TGACCCAGCA 
GATGACCCTG CCAACCGCAC CTTCAGGGGA AAGGTGAAGG CCTTCGAGCA GATGGACCAC 
CTTGCCAGAG CCAAACGTAT GCTGGAGCTG CAGGAGGCCG AACAAGCCCG GCTGGAAATC 
TCTCAGAAAC ATCCAGATAT TTATGCAGTT CCTAACAAGC CAAAACCAAA CCAGAACAGA 
CCACAACCAA TTGGCTCCAG CTCAAACTCC GAGTCCCAGA GCTCCTCCAA AGCAATGTAT 
TCAGAGAGAG GCTCACACTA CCGGCACGAT GATGATGACG AAGAGGAGTA TCGCCGCCAG 
CTGGCGGACC AGACCCGGAG AGGATATTAC AGCACACAGA AATACAATGA CACAGAGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP2A ( XM_009305192.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303467.1 Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA.

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CloneID	ODa44613
Clone ID Related Accession (Same CDS sequence)	XM_009305192.2 , XM_009305192.3
Accession Version	XM_009305192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA AGAAATTCAT CACCATCATG CAAGCGGCCA TCGGGGTCGC ACCGCTCAAC 
AAACGAGAGC TGATGCCACC CGGCCGGAGG TTGTGGAGAC CCTCAGGTGA CCAGACCAAC 
CAAATTGAGT TGTTCCGCTC AAGCCGTTCC AACAAGAAAA GGTTTTCCTT GACCTACGAA 
GCTCAATATC GGTGTCTGCG ACGCCTCTCT ACTCCAAGCT TCCCGGCCCT TTTACAGAAC 
CCAGTCATGG AGGAGACTGT GTGGGAGCAG TACACTGTGA CCCTACAGCG GGACTCCAAG 
ATGGGCTTTG GCCTAGCGGT GTCTGGAGGA CGGGACAATC CAAACGAAGA AAGTGGAGAA 
ATGTCAATCG TCATATCTGA TGTGCTGCAA GGGGGTCCAG CCGATGGCTT GCTGTTTGAA 
AATGACAGAG TGGTCCAGGT CAACACTGTG CCGATGGACG GTGTCCCACA CTCCTTTGCA 
GTCCAGACTC TCCGCAAATG TGGCAAAGTT GCCAAAATTA CTGTAAAGCG ATGTAGAAAA 
ATCCCTGTCA ACCTGACCAA GCGTGCTGCA TCCCCTGATG ACCGTGTCTT CAGTGGCGAC 
TACAACGATG ATTATGACTA TGAACAGGAC CAGCACAGCA TCTACAGTGG ACGCAGCTAC 
CCTAACCGTG ATACTAGTTT AGAGCGTGAG CGCGGCGGCT ATGCTGACGA GCGTGAACGG 
GACTATGATC GCAGTCGGGG GAGGAGCATG GAGCGCGGCG TGAGCCCTGA TAGACAATAC 
AGGCGGGACG GCAGCCGCGG ACGAATGCTG GACAACGAGC GCTGCCCCGA CCCCCGTTAC 
AGAAGTGAAA ATACCTTGGA TCGCGACCGC AGTCCAGACC CCCGTTATCG AAGTGAACGC 
ACGCTGGACC GCAACTACAG CCCAGAGCGA GGTCGCCGCT TTCGCAGCGA ACACAACCTG 
GACCGAGAGC GAAGTCCAGA CACACAATAC CGCAGCGATC GCACACTGGA CAGAACACAC 
AGTCCAGATA CACACTATAG GCCTGAACCT GCTCGGGGGT ACAGCAGAGA GAATCTCACC 
AGTGACAATG AGCGCAGGAG GTATGGCTCA CATCAGAACG AACCCATGAA GAAGAGCAAC 
AGCCAAGACA GATTGGATCG TTCGCCTTCA CCTGCACCAC TCCGACACAA TGCCTTGGAA 
AAGCCACTCA GTGTTACTCT CCTCAAAAAC CGTCCCAATG ATGAATATGG CCTTCGTCTA 
GGCAGTCAGT TGTTCATCAA AGAGATGACG AACACAGGCC TTGCATCCAG GGAAGGCAAA 
CTACAAGAAG GAGACATTAT TCTGAAAATC AACGGTACGG TCACAGAGAA TCTGTCTCTG 
AGCGATGCAG GGAAGCTGAT TGAGAAGTCT CGTGGAAAGC TGCAGCTGGT GGTGCAGAGG 
GATCGCAGTC AAGTGCTCAT CCGCATCCCA CCAATGGCAG ACAGCGACTC GGAAATGGAT 
GACATCTCTG AGATCGAGTC GTACCGCTCA TATTCTCCTC AGGAGGATCG GCGGAGTCAT 
CACTCAGACC TCTCCTCACA CTCCTCCAAT GAGAGATTAC AGGACAAAAA CAGAGAAGAG 
CCTCCTAAAA GACTGGCTAA AATGGGAGCG ATGCCCACTC CATTTGTATC AGCACCGGTG 
GATCTGCCAC CTCCACCTGT AGAGAAAGAA GAGCCTCGCA GCGAGTCTCC AGCTCCTCCT 
GTGGTAATTG CTGCTCCAAA AACTGTTCCA GTAAAAGCAA AACCTTTGCC AAAGGTTCCC 
TTGCTACCCA GTCCAGAAGA GCAGGAAATT TATGGGCCCA ACACTGTGAT GGTGCGCTTT 
GTGAAGGGTG AAAGCGTTGG TCTGCGTCTG GCTGGAGGAA ATGATGTGGG CATTTTCATC 
GCCGGCGTTC AAGAGGACAG TCCTGCCGAG GTGGAGGGTC TGCGCACTGG AGACCAAATA 
GTCAAGGTGA ATAACATGGA TTTTCGAGGC ATGGTCCGTG AAGATGCAGT TCTGTATTTA 
CTGGAGATTC CCAAAGGAGA GGATGTGACC ATTTTGGCTC AAAGCAAACC CGATGTGTAT 
AAAGACATCC TGGGGTCTGG ACGAGGTGAT AACTTCTTCA TCAGGACTCA TTTTGAATAT 
GAGAAAGAGC TTCCCCAGAG CCTGAACTTC TCTAGAGGAG AGGTTTTTAA AGTGGTGGAC 
ACCCTGTATG ATGGCAAGCT GGGAAACTGG CTCGCCATCC GCATGGACAA AGACAATCAG 
CTCCTGGAAA AGGGCATCAT ACCCAGCAAG AGCAGGGCGG AGCAGATGGC AAATGTTCAG 
CATGCACAGA AAGGTGCTGG TAATGACAGA GGCGATTTCT GGAGGCTCAG AGGTCAAAGG 
GCAGCTAAGA AAAAAGACCT TCGCAAGAGC AGAGAGGATC TCAGTGCAGC TCCAGTCACC 
ACACGCTTCC CGGCCTATGA GAGAGTGGTG CTGCGTGAAG CTGGCTTCAG GCGTCCAGTC 
GTGATTTTTG GCCCCATTTC AGATGCAGCC AACGAAAAAC TTGCCAATGA ACTTCCAAAT 
GAGTTCACCA TTGCAAAGAC CGAACCAAAA GACGCAGGCT CAGAAAAGTC ATCTGGCGTT 
GTGAGGCTAA ACACCATTCG TCAGATCATC GAACAGGATC TACATGCTCT GCTGGATGTC 
ACTCCAAAGG CTGTAGACAC CCTCAATTAC ACTCAGTGGT ATCCCATTGT CATCTTCCTC 
AACCCAGACA GCAAGCAGGG GGTGAAGACC ATGAGGAATC GCTTGGTGCC TGGATCCAAC 
CGCAGCTCAC GCAAACTTTA CGAACAAGCG GTCAAACTGC GGAAGACATA TGCGCATCTG 
TTCACAGCTA CCATTGATCT GAACTCTTCT CACGATGCCT GGTTTGGAAG CCTCAAAGAC 
GCCGTACGAG AACAGCAAGA TAAAGCTGTC TGGGTCTGTC AAAGCAAGCT GGATGGCTCA 
GAGGAGGACC TGGATCTCCA TGATGACCGT ATGTCCTACC TGTCGGCCAT GAGTGCAGAT 
TACCTGAGCA TGGACAGCCG TCTGACCAGC GACTATGAGG ACACGGCGGA CGAGGGCGGC 
GCATACACTG ATAATGAGCT TGATGAGTCC ACAGATGAGC CTCAGCCAAT GTCTGCCATC 
AGCCGCTCAT CTGAACCCGT CAGTGAGGAG AGGCACATGC CTGTTCCCCA CGAGCGCTCA 
AAGAAACCAT CGGGCAGAGA CGTCCAGCGT GACCCCAGTC CTCCTCCGTC ATTTGTCCCA 
GAACCCCCAA AGGTGCGCTC TGCTTCCCGT CCTGCAGACT CCAGGAGTTT TGACTCGCGC 
TCCAGCAGCA CCATCAGCAG TGATGCGCCC ATCAGCAACA AACCTCTTCC TCCGCCCGTC 
GCCCAGAAGC CCAGTTTCAA TACGAGGTCG GCGGACGACA CACCTTCTAA TGACCCAGCA 
GATGACCCTG CCAACCGCAC CTTCAGGGGA AAGGTGAAGG CCTTCGAGCA GATGGACCAC 
CTTGCCAGAG CCAAACGTAT GCTGGAGCTG CAGGAGGCCG AACAAGCCCG GCTGGAAATC 
TCTCAGAAAC ATCCAGATAT TTATGCAGTT CCTAACAAGC CAAAACCAAA CCAGAACAGA 
CCACAACCAA TTGGCTCCAG CTCAAACTCC GAGTCCCAGA GCTCCTCCAA AGCAATGTAT 
TCAGAGAGAG GCTCACACTA CCGGCACGAT GATGATGACG AAGAGGAGTA TCGCCGCCAG 
CTGGCGGACC AGACCCGGAG AGGATATTAC AGCACACAGA AATACAATGA CACAGAGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303467.1
CDS	226..4068
Translation	MKYKKFITIMQAAIGVAPLNKRELMPPGRRLWRPSGDQTNQIELFRSSRSNKKRFSLTYEAQYRCLRRLSTPSFPALLQNPVMEETVWEQYTVTLQRDSKMGFGLAVSGGRDNPNEESGEMSIVISDVLQGGPADGLLFENDRVVQVNTVPMDGVPHSFAVQTLRKCGKVAKITVKRCRKIPVNLTKRAASPDDRVFSGDYNDDYDYEQDQHSIYSGRSYPNRDTSLERERGGYADERERDYDRSRGRSMERGVSPDRQYRRDGSRGRMLDNERCPDPRYRSENTLDRDRSPDPRYRSERTLDRNYSPERGRRFRSEHNLDRERSPDTQYRSDRTLDRTHSPDTHYRPEPARGYSRENLTSDNERRRYGSHQNEPMKKSNSQDRLDRSPSPAPLRHNALEKPLSVTLLKNRPNDEYGLRLGSQLFIKEMTNTGLASREGKLQEGDIILKINGTVTENLSLSDAGKLIEKSRGKLQLVVQRDRSQVLIRIPPMADSDSEMDDISEIESYRSYSPQEDRRSHHSDLSSHSSNERLQDKNREEPPKRLAKMGAMPTPFVSAPVDLPPPPVEKEEPRSESPAPPVVIAAPKTVPVKAKPLPKVPLLPSPEEQEIYGPNTVMVRFVKGESVGLRLAGGNDVGIFIAGVQEDSPAEVEGLRTGDQIVKVNNMDFRGMVREDAVLYLLEIPKGEDVTILAQSKPDVYKDILGSGRGDNFFIRTHFEYEKELPQSLNFSRGEVFKVVDTLYDGKLGNWLAIRMDKDNQLLEKGIIPSKSRAEQMANVQHAQKGAGNDRGDFWRLRGQRAAKKKDLRKSREDLSAAPVTTRFPAYERVVLREAGFRRPVVIFGPISDAANEKLANELPNEFTIAKTEPKDAGSEKSSGVVRLNTIRQIIEQDLHALLDVTPKAVDTLNYTQWYPIVIFLNPDSKQGVKTMRNRLVPGSNRSSRKLYEQAVKLRKTYAHLFTATIDLNSSHDAWFGSLKDAVREQQDKAVWVCQSKLDGSEEDLDLHDDRMSYLSAMSADYLSMDSRLTSDYEDTADEGGAYTDNELDESTDEPQPMSAISRSSEPVSEERHMPVPHERSKKPSGRDVQRDPSPPPSFVPEPPKVRSASRPADSRSFDSRSSSTISSDAPISNKPLPPPVAQKPSFNTRSADDTPSNDPADDPANRTFRGKVKAFEQMDHLARAKRMLELQEAEQARLEISQKHPDIYAVPNKPKPNQNRPQPIGSSSNSESQSSSKAMYSERGSHYRHDDDDEEEYRRQLADQTRRGYYSTQKYNDTEL

Target ORF information:

RefSeq Version	XM_009305192.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305192.3

ORF Insert Sequence:

ATGAAGTACA AGAAATTCAT CACCATCATG CAAGCGGCCA TCGGGGTCGC ACCGCTCAAC 
AAACGAGAGC TGATGCCACC CGGCCGGAGG TTGTGGAGAC CCTCAGGTGA CCAGACCAAC 
CAAATTGAGT TGTTCCGCTC AAGCCGTTCC AACAAGAAAA GGTTTTCCTT GACCTACGAA 
GCTCAATATC GGTGTCTGCG ACGCCTCTCT ACTCCAAGCT TCCCGGCCCT TTTACAGAAC 
CCAGTCATGG AGGAGACTGT GTGGGAGCAG TACACTGTGA CCCTACAGCG GGACTCCAAG 
ATGGGCTTTG GCCTAGCGGT GTCTGGAGGA CGGGACAATC CAAACGAAGA AAGTGGAGAA 
ATGTCAATCG TCATATCTGA TGTGCTGCAA GGGGGTCCAG CCGATGGCTT GCTGTTTGAA 
AATGACAGAG TGGTCCAGGT CAACACTGTG CCGATGGACG GTGTCCCACA CTCCTTTGCA 
GTCCAGACTC TCCGCAAATG TGGCAAAGTT GCCAAAATTA CTGTAAAGCG ATGTAGAAAA 
ATCCCTGTCA ACCTGACCAA GCGTGCTGCA TCCCCTGATG ACCGTGTCTT CAGTGGCGAC 
TACAACGATG ATTATGACTA TGAACAGGAC CAGCACAGCA TCTACAGTGG ACGCAGCTAC 
CCTAACCGTG ATACTAGTTT AGAGCGTGAG CGCGGCGGCT ATGCTGACGA GCGTGAACGG 
GACTATGATC GCAGTCGGGG GAGGAGCATG GAGCGCGGCG TGAGCCCTGA TAGACAATAC 
AGGCGGGACG GCAGCCGCGG ACGAATGCTG GACAACGAGC GCTGCCCCGA CCCCCGTTAC 
AGAAGTGAAA ATACCTTGGA TCGCGACCGC AGTCCAGACC CCCGTTATCG AAGTGAACGC 
ACGCTGGACC GCAACTACAG CCCAGAGCGA GGTCGCCGCT TTCGCAGCGA ACACAACCTG 
GACCGAGAGC GAAGTCCAGA CACACAATAC CGCAGCGATC GCACACTGGA CAGAACACAC 
AGTCCAGATA CACACTATAG GCCTGAACCT GCTCGGGGGT ACAGCAGAGA GAATCTCACC 
AGTGACAATG AGCGCAGGAG GTATGGCTCA CATCAGAACG AACCCATGAA GAAGAGCAAC 
AGCCAAGACA GATTGGATCG TTCGCCTTCA CCTGCACCAC TCCGACACAA TGCCTTGGAA 
AAGCCACTCA GTGTTACTCT CCTCAAAAAC CGTCCCAATG ATGAATATGG CCTTCGTCTA 
GGCAGTCAGT TGTTCATCAA AGAGATGACG AACACAGGCC TTGCATCCAG GGAAGGCAAA 
CTACAAGAAG GAGACATTAT TCTGAAAATC AACGGTACGG TCACAGAGAA TCTGTCTCTG 
AGCGATGCAG GGAAGCTGAT TGAGAAGTCT CGTGGAAAGC TGCAGCTGGT GGTGCAGAGG 
GATCGCAGTC AAGTGCTCAT CCGCATCCCA CCAATGGCAG ACAGCGACTC GGAAATGGAT 
GACATCTCTG AGATCGAGTC GTACCGCTCA TATTCTCCTC AGGAGGATCG GCGGAGTCAT 
CACTCAGACC TCTCCTCACA CTCCTCCAAT GAGAGATTAC AGGACAAAAA CAGAGAAGAG 
CCTCCTAAAA GACTGGCTAA AATGGGAGCG ATGCCCACTC CATTTGTATC AGCACCGGTG 
GATCTGCCAC CTCCACCTGT AGAGAAAGAA GAGCCTCGCA GCGAGTCTCC AGCTCCTCCT 
GTGGTAATTG CTGCTCCAAA AACTGTTCCA GTAAAAGCAA AACCTTTGCC AAAGGTTCCC 
TTGCTACCCA GTCCAGAAGA GCAGGAAATT TATGGGCCCA ACACTGTGAT GGTGCGCTTT 
GTGAAGGGTG AAAGCGTTGG TCTGCGTCTG GCTGGAGGAA ATGATGTGGG CATTTTCATC 
GCCGGCGTTC AAGAGGACAG TCCTGCCGAG GTGGAGGGTC TGCGCACTGG AGACCAAATA 
GTCAAGGTGA ATAACATGGA TTTTCGAGGC ATGGTCCGTG AAGATGCAGT TCTGTATTTA 
CTGGAGATTC CCAAAGGAGA GGATGTGACC ATTTTGGCTC AAAGCAAACC CGATGTGTAT 
AAAGACATCC TGGGGTCTGG ACGAGGTGAT AACTTCTTCA TCAGGACTCA TTTTGAATAT 
GAGAAAGAGC TTCCCCAGAG CCTGAACTTC TCTAGAGGAG AGGTTTTTAA AGTGGTGGAC 
ACCCTGTATG ATGGCAAGCT GGGAAACTGG CTCGCCATCC GCATGGACAA AGACAATCAG 
CTCCTGGAAA AGGGCATCAT ACCCAGCAAG AGCAGGGCGG AGCAGATGGC AAATGTTCAG 
CATGCACAGA AAGGTGCTGG TAATGACAGA GGCGATTTCT GGAGGCTCAG AGGTCAAAGG 
GCAGCTAAGA AAAAAGACCT TCGCAAGAGC AGAGAGGATC TCAGTGCAGC TCCAGTCACC 
ACACGCTTCC CGGCCTATGA GAGAGTGGTG CTGCGTGAAG CTGGCTTCAG GCGTCCAGTC 
GTGATTTTTG GCCCCATTTC AGATGCAGCC AACGAAAAAC TTGCCAATGA ACTTCCAAAT 
GAGTTCACCA TTGCAAAGAC CGAACCAAAA GACGCAGGCT CAGAAAAGTC ATCTGGCGTT 
GTGAGGCTAA ACACCATTCG TCAGATCATC GAACAGGATC TACATGCTCT GCTGGATGTC 
ACTCCAAAGG CTGTAGACAC CCTCAATTAC ACTCAGTGGT ATCCCATTGT CATCTTCCTC 
AACCCAGACA GCAAGCAGGG GGTGAAGACC ATGAGGAATC GCTTGGTGCC TGGATCCAAC 
CGCAGCTCAC GCAAACTTTA CGAACAAGCG GTCAAACTGC GGAAGACATA TGCGCATCTG 
TTCACAGCTA CCATTGATCT GAACTCTTCT CACGATGCCT GGTTTGGAAG CCTCAAAGAC 
GCCGTACGAG AACAGCAAGA TAAAGCTGTC TGGGTCTGTC AAAGCAAGCT GGATGGCTCA 
GAGGAGGACC TGGATCTCCA TGATGACCGT ATGTCCTACC TGTCGGCCAT GAGTGCAGAT 
TACCTGAGCA TGGACAGCCG TCTGACCAGC GACTATGAGG ACACGGCGGA CGAGGGCGGC 
GCATACACTG ATAATGAGCT TGATGAGTCC ACAGATGAGC CTCAGCCAAT GTCTGCCATC 
AGCCGCTCAT CTGAACCCGT CAGTGAGGAG AGGCACATGC CTGTTCCCCA CGAGCGCTCA 
AAGAAACCAT CGGGCAGAGA CGTCCAGCGT GACCCCAGTC CTCCTCCGTC ATTTGTCCCA 
GAACCCCCAA AGGTGCGCTC TGCTTCCCGT CCTGCAGACT CCAGGAGTTT TGACTCGCGC 
TCCAGCAGCA CCATCAGCAG TGATGCGCCC ATCAGCAACA AACCTCTTCC TCCGCCCGTC 
GCCCAGAAGC CCAGTTTCAA TACGAGGTCG GCGGACGACA CACCTTCTAA TGACCCAGCA 
GATGACCCTG CCAACCGCAC CTTCAGGGGA AAGGTGAAGG CCTTCGAGCA GATGGACCAC 
CTTGCCAGAG CCAAACGTAT GCTGGAGCTG CAGGAGGCCG AACAAGCCCG GCTGGAAATC 
TCTCAGAAAC ATCCAGATAT TTATGCAGTT CCTAACAAGC CAAAACCAAA CCAGAACAGA 
CCACAACCAA TTGGCTCCAG CTCAAACTCC GAGTCCCAGA GCTCCTCCAA AGCAATGTAT 
TCAGAGAGAG GCTCACACTA CCGGCACGAT GATGATGACG AAGAGGAGTA TCGCCGCCAG 
CTGGCGGACC AGACCCGGAG AGGATATTAC AGCACACAGA AATACAATGA CACAGAGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth.
Disease models & mechanisms10(1)15-28(2017 01)
Mayrhofer M,Gourain V,Reischl M,Affaticati P,Jenett A,Joly JS,Benelli M,Demichelis F,Poliani PL,Sieger D,Mione M

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

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TJP2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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