TJP2A cDNA ORF clone, Danio rerio(Zebrafish)

The following TJP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa00390 NM_001201570.1
Latest version!
Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa35091 NM_001201571.1
Latest version!
Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa15901 XM_005155574.4
Latest version!
Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa44613 XM_009305192.3
Latest version!
Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa44613 XM_009305192.2 Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa15901 XM_005155574.3 Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa00390
    Clone ID Related Accession (Same CDS sequence) NM_001201570.1
    Accession Version NM_001201570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-02
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX640481.14 and BX629350.6. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGTCG TTGTGGAGAA ACGGCAGTTT CTGTGTAGAA ATGTTAAGGA CTGTTATTAT 
    AACCCAGTCA TGGAGGAGAC TGTGTGGGAG CAGTACACTG TGACCCTACA GCGGGACTCC
    AAGATGGGCT TTGGCCTAGC GGTGTCTGGA GGACGGGACA ATCCAAACGA AGAAAGTGGA
    GAAATGTCAA TCGTCATATC TGATGTGCTG CAAGGGGGTC CAGCCGATGG CTTGCTGTTT
    GAAAATGACA GAGTGGTCCA GGTCAACACT GTGCCGATGG ACGGTGTCCC ACACTCCTTT
    GCAGTCCAGA CTCTCCGCAA ATGTGGCAAA GTTGCCAAAA TTACTGTAAA GCGATGTAGA
    AAAATCCCTG TCAACCTGAC CAAGCGTGCT GCATCCCCTG ATGACCGTGT CTTCAGTGGC
    GACTACAACG ATGATTATGA CTATGAACAG GACCAGCACA GCATCTACAG TGGACGCAGC
    TACCCTAACC GTGATACTAG TTTAGAGCGT GAGCGCGGCG GCTATGCTGA CGAGCGTGAA
    CGGGACTATG ATCGCAGTCG GGGGAGGAGC ATGGAGCGCG GCGTGAGCCC TGATAGACAA
    TACAGGCGGG ACGGCAGCCG CGGACGAATG CTGGACAACG AGCGCTGCCC CGACCCCCGT
    TACAGAAGTG AAAATACCTT GGATCGCGAC CGCAGTCCAG ACCCCCGTTA TCGAAGTGAA
    CGCACGCTGG ACCGCAACTA CAGCCCAGAG CGAGGTCGCC GCTTTCGCAG CGAACACAAC
    CTGGACCGAG AGCGAAGTCC AGACACACAA TACCGCAGCG ATCGCACACT GGACAGAACA
    CACAGTCCAG ATACACACTA TAGGCCTGAA CCTGCTCGGG GGTACAGCAG AGAGAATCTC
    ACCAGTGACA ATGAGCGCAG GAGGTATGGC TCACATCAGA ACGAACCCAT GAAGAAGAGC
    AACAGCCAAG ACAGATTGGA TCGTTCGCCT TCACCTGCAC CACTCCGACA CAATGCCTTG
    GAAAAGCCAC TCAGTGTTAC TCTCCTCAAA AACCGTCCCA ATGATGAATA TGGCCTTCGT
    CTAGGCAGTC AGTTGTTCAT CAAAGAGATG ACGAACACAG GCCTTGCATC CAGGGAAGGC
    AAACTACAAG AAGGAGACAT TATTCTGAAA ATCAACGGTA CGGTCACAGA GAATCTGTCT
    CTGAGCGATG CAGGGAAGCT GATTGAGAAG TCTCGTGGAA AGCTGCAGCT GGTGGTGCAG
    AGGGATCGCA GTCAAGTGCT CATCCGCATC CCACCAATGG CAGACAGCGA CTCGGAAATG
    GATGACATCT CTGAGATCGA GTCGTACCGC TCATATTCTC CTCAGGAGGA TCGGCGGAGT
    CATCACTCAG ACCTCTCCTC ACACTCCTCC AATGAGAGAT TACAGGACAA AAACAGAGAA
    GAGCCTCCTA AAAGACTGGC TAAAATGGGA GCGATGCCCA CTCCATTTGT ATCAGCACCG
    GTGGATCTGC CACCTCCACC TGTAGAGAAA GAAGAGCCTC GCAGCGAGTC TCCAGCTCCT
    CCTGTGGTAA TTGCTGCTCC AAAAACTGTT CCAGTAAAAG CAAAACCTTT GCCAAAGGTT
    CCCTTGCTAC CCAGTCCAGA AGAGCAGGAA ATTTATGGGC CCAACACTGT GATGGTGCGC
    TTTGTGAAGG GTGAAAGCGT TGGTCTGCGT CTGGCTGGAG GAAATGATGT GGGCATTTTC
    ATCGCCGGCG TTCAAGAGGA CAGTCCTGCC GAGGTGGAGG GTCTGCGCAC TGGAGACCAA
    ATAGTCAAGG TGAATAACAT GGATTTTCGA GGCATGGTCC GTGAAGATGC AGTTCTGTAT
    TTACTGGAGA TTCCCAAAGG AGAGGATGTG ACCATTTTGG CTCAAAGCAA ACCCGATGTG
    TATAAAGACA TCCTGGGGTC TGGACGAGGT GATAACTTCT TCATCAGGAC TCATTTTGAA
    TATGAGAAAG AGCTTCCCCA GAGCCTGAAC TTCTCTAGAG GAGAGGTTTT TAAAGTGGTG
    GACACCCTGT ATGATGGCAA GCTGGGAAAC TGGCTCGCCA TCCGCATGGA CAAAGACAAT
    CAGCTCCTGG AAAAGGGCAT CATACCCAGC AAGAGCAGGG CGGAGCAGAT GGCAAATGTT
    CAGCATGCAC AGAAAGGTGC TGGTAATGAC AGAGGCGATT TCTGGAGGCT CAGAGGTCAA
    AGGGCAGCTA AGAAAAAAGA CCTTCGCAAG AGCAGAGAGG ATCTCAGTGC AGCTCCAGTC
    ACCACACGCT TCCCGGCCTA TGAGAGAGTG GTGCTGCGTG AAGCTGGCTT CAGGCGTCCA
    GTCGTGATTT TTGGCCCCAT TTCAGATGCA GCCAACGAAA AACTTGCCAA TGAACTTCCA
    AATGAGTTCA CCATTGCAAA GACCGAACCA AAAGACGCAG GCTCAGAAAA GTCATCTGGC
    GTTGTGAGGC TAAACACCAT TCGTCAGATC ATCGAACAGG ATCTACATGC TCTGCTGGAT
    GTCACTCCAA AGGCTGTAGA CACCCTCAAT TACACTCAGT GGTATCCCAT TGTCATCTTC
    CTCAACCCAG ACAGCAAGCA GGGGGTGAAG ACCATGAGGA ATCGCTTGGT GCCTGGATCC
    AACCGCAGCT CACGCAAACT TTACGAACAA GCGGTCAAAC TGCGGAAGAC ATATGCGCAT
    CTGTTCACAG CTACCATTGA TCTGAACTCT TCTCACGATG CCTGGTTTGG AAGCCTCAAA
    GACGCCGTAC GAGAACAGCA AGATAAAGCT GTCTGGGTCT GTCAAAGCAA GCTGGATGGC
    TCAGAGGAGG ACCTGGATCT CCATGATGAC CGTATGTCCT ACCTGTCGGC CATGAGTGCA
    GATTACCTGA GCATGGACAG CCGTCTGACC AGCGACTATG AGGACACGGC GGACGAGGGC
    GGCGCATACA CTGATAATGA GCTTGATGAG TCCACAGATG AGCCTCAGCC AATGTCTGCC
    ATCAGCCGCT CATCTGAACC CGTCAGTGAG GAGAGGCACA TGCCTGTTCC CCACGAGCGC
    TCAAAGAAAC CATCGGGCAG AGACGTCCAG CGTGACCCCA GTCCTCCTCC GTCATTTGTC
    CCAGAACCCC CAAAGGTGCG CTCTGCTTCC CGTCCTGCAG ACTCCAGGAG TTTTGACTCG
    CGCTCCAGCA GCACCATCAG CAGTGATGCG CCCATCAGCA ACAAACCTCT TCCTCCGCCC
    GTCGCCCAGA AGCCCAGTTT CAATACGAGG TCGGCGGACG ACACACCTTC TAATGACCCA
    GCAGATGACC CTGCCAACCG CACCTTCAGG GGAAAGGTGA AGGCCTTCGA GCAGATGGAC
    CACCTTGCCA GAGCCAAACG TATGCTGGAG CTGCAGGAGG CCGAACAAGC CCGGCTGGAA
    ATCTCTCAGA AACATCCAGA TATTTATGCA GTTCCTAACA AGCCAAAACC AAACCAGAAC
    AGACCACAAC CAATTGGCTC CAGCTCAAAC TCCGAGTCCC AGAGCTCCTC CAAAGCAATG
    TATTCAGAGA GAGGCTCACA CTACCGGCAC GATGATGATG ACGAAGAGGA GTATCGCCGC
    CAGCTGGCGG ACCAGACCCG GAGAGGATAT TACAGCACAC AGAAATACAA TGACACAGAG
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188499.1
    CDS145..3810
    Translation

    Target ORF information:

    RefSeq Version NM_001201570.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGTCG TTGTGGAGAA ACGGCAGTTT CTGTGTAGAA ATGTTAAGGA CTGTTATTAT 
    AACCCAGTCA TGGAGGAGAC TGTGTGGGAG CAGTACACTG TGACCCTACA GCGGGACTCC
    AAGATGGGCT TTGGCCTAGC GGTGTCTGGA GGACGGGACA ATCCAAACGA AGAAAGTGGA
    GAAATGTCAA TCGTCATATC TGATGTGCTG CAAGGGGGTC CAGCCGATGG CTTGCTGTTT
    GAAAATGACA GAGTGGTCCA GGTCAACACT GTGCCGATGG ACGGTGTCCC ACACTCCTTT
    GCAGTCCAGA CTCTCCGCAA ATGTGGCAAA GTTGCCAAAA TTACTGTAAA GCGATGTAGA
    AAAATCCCTG TCAACCTGAC CAAGCGTGCT GCATCCCCTG ATGACCGTGT CTTCAGTGGC
    GACTACAACG ATGATTATGA CTATGAACAG GACCAGCACA GCATCTACAG TGGACGCAGC
    TACCCTAACC GTGATACTAG TTTAGAGCGT GAGCGCGGCG GCTATGCTGA CGAGCGTGAA
    CGGGACTATG ATCGCAGTCG GGGGAGGAGC ATGGAGCGCG GCGTGAGCCC TGATAGACAA
    TACAGGCGGG ACGGCAGCCG CGGACGAATG CTGGACAACG AGCGCTGCCC CGACCCCCGT
    TACAGAAGTG AAAATACCTT GGATCGCGAC CGCAGTCCAG ACCCCCGTTA TCGAAGTGAA
    CGCACGCTGG ACCGCAACTA CAGCCCAGAG CGAGGTCGCC GCTTTCGCAG CGAACACAAC
    CTGGACCGAG AGCGAAGTCC AGACACACAA TACCGCAGCG ATCGCACACT GGACAGAACA
    CACAGTCCAG ATACACACTA TAGGCCTGAA CCTGCTCGGG GGTACAGCAG AGAGAATCTC
    ACCAGTGACA ATGAGCGCAG GAGGTATGGC TCACATCAGA ACGAACCCAT GAAGAAGAGC
    AACAGCCAAG ACAGATTGGA TCGTTCGCCT TCACCTGCAC CACTCCGACA CAATGCCTTG
    GAAAAGCCAC TCAGTGTTAC TCTCCTCAAA AACCGTCCCA ATGATGAATA TGGCCTTCGT
    CTAGGCAGTC AGTTGTTCAT CAAAGAGATG ACGAACACAG GCCTTGCATC CAGGGAAGGC
    AAACTACAAG AAGGAGACAT TATTCTGAAA ATCAACGGTA CGGTCACAGA GAATCTGTCT
    CTGAGCGATG CAGGGAAGCT GATTGAGAAG TCTCGTGGAA AGCTGCAGCT GGTGGTGCAG
    AGGGATCGCA GTCAAGTGCT CATCCGCATC CCACCAATGG CAGACAGCGA CTCGGAAATG
    GATGACATCT CTGAGATCGA GTCGTACCGC TCATATTCTC CTCAGGAGGA TCGGCGGAGT
    CATCACTCAG ACCTCTCCTC ACACTCCTCC AATGAGAGAT TACAGGACAA AAACAGAGAA
    GAGCCTCCTA AAAGACTGGC TAAAATGGGA GCGATGCCCA CTCCATTTGT ATCAGCACCG
    GTGGATCTGC CACCTCCACC TGTAGAGAAA GAAGAGCCTC GCAGCGAGTC TCCAGCTCCT
    CCTGTGGTAA TTGCTGCTCC AAAAACTGTT CCAGTAAAAG CAAAACCTTT GCCAAAGGTT
    CCCTTGCTAC CCAGTCCAGA AGAGCAGGAA ATTTATGGGC CCAACACTGT GATGGTGCGC
    TTTGTGAAGG GTGAAAGCGT TGGTCTGCGT CTGGCTGGAG GAAATGATGT GGGCATTTTC
    ATCGCCGGCG TTCAAGAGGA CAGTCCTGCC GAGGTGGAGG GTCTGCGCAC TGGAGACCAA
    ATAGTCAAGG TGAATAACAT GGATTTTCGA GGCATGGTCC GTGAAGATGC AGTTCTGTAT
    TTACTGGAGA TTCCCAAAGG AGAGGATGTG ACCATTTTGG CTCAAAGCAA ACCCGATGTG
    TATAAAGACA TCCTGGGGTC TGGACGAGGT GATAACTTCT TCATCAGGAC TCATTTTGAA
    TATGAGAAAG AGCTTCCCCA GAGCCTGAAC TTCTCTAGAG GAGAGGTTTT TAAAGTGGTG
    GACACCCTGT ATGATGGCAA GCTGGGAAAC TGGCTCGCCA TCCGCATGGA CAAAGACAAT
    CAGCTCCTGG AAAAGGGCAT CATACCCAGC AAGAGCAGGG CGGAGCAGAT GGCAAATGTT
    CAGCATGCAC AGAAAGGTGC TGGTAATGAC AGAGGCGATT TCTGGAGGCT CAGAGGTCAA
    AGGGCAGCTA AGAAAAAAGA CCTTCGCAAG AGCAGAGAGG ATCTCAGTGC AGCTCCAGTC
    ACCACACGCT TCCCGGCCTA TGAGAGAGTG GTGCTGCGTG AAGCTGGCTT CAGGCGTCCA
    GTCGTGATTT TTGGCCCCAT TTCAGATGCA GCCAACGAAA AACTTGCCAA TGAACTTCCA
    AATGAGTTCA CCATTGCAAA GACCGAACCA AAAGACGCAG GCTCAGAAAA GTCATCTGGC
    GTTGTGAGGC TAAACACCAT TCGTCAGATC ATCGAACAGG ATCTACATGC TCTGCTGGAT
    GTCACTCCAA AGGCTGTAGA CACCCTCAAT TACACTCAGT GGTATCCCAT TGTCATCTTC
    CTCAACCCAG ACAGCAAGCA GGGGGTGAAG ACCATGAGGA ATCGCTTGGT GCCTGGATCC
    AACCGCAGCT CACGCAAACT TTACGAACAA GCGGTCAAAC TGCGGAAGAC ATATGCGCAT
    CTGTTCACAG CTACCATTGA TCTGAACTCT TCTCACGATG CCTGGTTTGG AAGCCTCAAA
    GACGCCGTAC GAGAACAGCA AGATAAAGCT GTCTGGGTCT GTCAAAGCAA GCTGGATGGC
    TCAGAGGAGG ACCTGGATCT CCATGATGAC CGTATGTCCT ACCTGTCGGC CATGAGTGCA
    GATTACCTGA GCATGGACAG CCGTCTGACC AGCGACTATG AGGACACGGC GGACGAGGGC
    GGCGCATACA CTGATAATGA GCTTGATGAG TCCACAGATG AGCCTCAGCC AATGTCTGCC
    ATCAGCCGCT CATCTGAACC CGTCAGTGAG GAGAGGCACA TGCCTGTTCC CCACGAGCGC
    TCAAAGAAAC CATCGGGCAG AGACGTCCAG CGTGACCCCA GTCCTCCTCC GTCATTTGTC
    CCAGAACCCC CAAAGGTGCG CTCTGCTTCC CGTCCTGCAG ACTCCAGGAG TTTTGACTCG
    CGCTCCAGCA GCACCATCAG CAGTGATGCG CCCATCAGCA ACAAACCTCT TCCTCCGCCC
    GTCGCCCAGA AGCCCAGTTT CAATACGAGG TCGGCGGACG ACACACCTTC TAATGACCCA
    GCAGATGACC CTGCCAACCG CACCTTCAGG GGAAAGGTGA AGGCCTTCGA GCAGATGGAC
    CACCTTGCCA GAGCCAAACG TATGCTGGAG CTGCAGGAGG CCGAACAAGC CCGGCTGGAA
    ATCTCTCAGA AACATCCAGA TATTTATGCA GTTCCTAACA AGCCAAAACC AAACCAGAAC
    AGACCACAAC CAATTGGCTC CAGCTCAAAC TCCGAGTCCC AGAGCTCCTC CAAAGCAATG
    TATTCAGAGA GAGGCTCACA CTACCGGCAC GATGATGATG ACGAAGAGGA GTATCGCCGC
    CAGCTGGCGG ACCAGACCCG GAGAGGATAT TACAGCACAC AGAAATACAA TGACACAGAG
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa35091
    Clone ID Related Accession (Same CDS sequence) NM_001201571.1
    Accession Version NM_001201571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-02
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX640481.14 and BX629350.6. On Jan 28, 2011 this sequence version replaced XM_688726.4. Transcript Variant: This variant (2) lacks the first 5' exon, includes two non-coding 5' exons and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01002488.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGAGA CTGTGTGGGA GCAGTACACT GTGACCCTAC AGCGGGACTC CAAGATGGGC 
    TTTGGCCTAG CGGTGTCTGG AGGACGGGAC AATCCAAACG AAGAAAGTGG AGAAATGTCA
    ATCGTCATAT CTGATGTGCT GCAAGGGGGT CCAGCCGATG GCTTGCTGTT TGAAAATGAC
    AGAGTGGTCC AGGTCAACAC TGTGCCGATG GACGGTGTCC CACACTCCTT TGCAGTCCAG
    ACTCTCCGCA AATGTGGCAA AGTTGCCAAA ATTACTGTAA AGCGATGTAG AAAAATCCCT
    GTCAACCTGA CCAAGCGTGC TGCATCCCCT GATGACCGTG TCTTCAGTGG CGACTACAAC
    GATGATTATG ACTATGAACA GGACCAGCAC AGCATCTACA GTGGACGCAG CTACCCTAAC
    CGTGATACTA GTTTAGAGCG TGAGCGCGGC GGCTATGCTG ACGAGCGTGA ACGGGACTAT
    GATCGCAGTC GGGGGAGGAG CATGGAGCGC GGCGTGAGCC CTGATAGACA ATACAGGCGG
    GACGGCAGCC GCGGACGAAT GCTGGACAAC GAGCGCTGCC CCGACCCCCG TTACAGAAGT
    GAAAATACCT TGGATCGCGA CCGCAGTCCA GACCCCCGTT ATCGAAGTGA ACGCACGCTG
    GACCGCAACT ACAGCCCAGA GCGAGGTCGC CGCTTTCGCA GCGAACACAA CCTGGACCGA
    GAGCGAAGTC CAGACACACA ATACCGCAGC GATCGCACAC TGGACAGAAC ACACAGTCCA
    GATACACACT ATAGGCCTGA ACCTGCTCGG GGGTACAGCA GAGAGAATCT CACCAGTGAC
    AATGAGCGCA GGAGGTATGG CTCACATCAG AACGAACCCA TGAAGAAGAG CAACAGCCAA
    GACAGATTGG ATCGTTCGCC TTCACCTGCA CCACTCCGAC ACAATGCCTT GGAAAAGCCA
    CTCAGTGTTA CTCTCCTCAA AAACCGTCCC AATGATGAAT ATGGCCTTCG TCTAGGCAGT
    CAGTTGTTCA TCAAAGAGAT GACGAACACA GGCCTTGCAT CCAGGGAAGG CAAACTACAA
    GAAGGAGACA TTATTCTGAA AATCAACGGT ACGGTCACAG AGAATCTGTC TCTGAGCGAT
    GCAGGGAAGC TGATTGAGAA GTCTCGTGGA AAGCTGCAGC TGGTGGTGCA GAGGGATCGC
    AGTCAAGTGC TCATCCGCAT CCCACCAATG GCAGACAGCG ACTCGGAAAT GGATGACATC
    TCTGAGATCG AGTCGTACCG CTCATATTCT CCTCAGGAGG ATCGGCGGAG TCATCACTCA
    GACCTCTCCT CACACTCCTC CAATGAGAGA TTACAGGACA AAAACAGAGA AGAGCCTCCT
    AAAAGACTGG CTAAAATGGG AGCGATGCCC ACTCCATTTG TATCAGCACC GGTGGATCTG
    CCACCTCCAC CTGTAGAGAA AGAAGAGCCT CGCAGCGAGT CTCCAGCTCC TCCTGTGGTA
    ATTGCTGCTC CAAAAACTGT TCCAGTAAAA GCAAAACCTT TGCCAAAGGT TCCCTTGCTA
    CCCAGTCCAG AAGAGCAGGA AATTTATGGG CCCAACACTG TGATGGTGCG CTTTGTGAAG
    GGTGAAAGCG TTGGTCTGCG TCTGGCTGGA GGAAATGATG TGGGCATTTT CATCGCCGGC
    GTTCAAGAGG ACAGTCCTGC CGAGGTGGAG GGTCTGCGCA CTGGAGACCA AATAGTCAAG
    GTGAATAACA TGGATTTTCG AGGCATGGTC CGTGAAGATG CAGTTCTGTA TTTACTGGAG
    ATTCCCAAAG GAGAGGATGT GACCATTTTG GCTCAAAGCA AACCCGATGT GTATAAAGAC
    ATCCTGGGGT CTGGACGAGG TGATAACTTC TTCATCAGGA CTCATTTTGA ATATGAGAAA
    GAGCTTCCCC AGAGCCTGAA CTTCTCTAGA GGAGAGGTTT TTAAAGTGGT GGACACCCTG
    TATGATGGCA AGCTGGGAAA CTGGCTCGCC ATCCGCATGG ACAAAGACAA TCAGCTCCTG
    GAAAAGGGCA TCATACCCAG CAAGAGCAGG GCGGAGCAGA TGGCAAATGT TCAGCATGCA
    CAGAAAGGTG CTGGTAATGA CAGAGGCGAT TTCTGGAGGC TCAGAGGTCA AAGGGCAGCT
    AAGAAAAAAG ACCTTCGCAA GAGCAGAGAG GATCTCAGTG CAGCTCCAGT CACCACACGC
    TTCCCGGCCT ATGAGAGAGT GGTGCTGCGT GAAGCTGGCT TCAGGCGTCC AGTCGTGATT
    TTTGGCCCCA TTTCAGATGC AGCCAACGAA AAACTTGCCA ATGAACTTCC AAATGAGTTC
    ACCATTGCAA AGACCGAACC AAAAGACGCA GGCTCAGAAA AGTCATCTGG CGTTGTGAGG
    CTAAACACCA TTCGTCAGAT CATCGAACAG GATCTACATG CTCTGCTGGA TGTCACTCCA
    AAGGCTGTAG ACACCCTCAA TTACACTCAG TGGTATCCCA TTGTCATCTT CCTCAACCCA
    GACAGCAAGC AGGGGGTGAA GACCATGAGG AATCGCTTGG TGCCTGGATC CAACCGCAGC
    TCACGCAAAC TTTACGAACA AGCGGTCAAA CTGCGGAAGA CATATGCGCA TCTGTTCACA
    GCTACCATTG ATCTGAACTC TTCTCACGAT GCCTGGTTTG GAAGCCTCAA AGACGCCGTA
    CGAGAACAGC AAGATAAAGC TGTCTGGGTC TGTCAAAGCA AGCTGGATGG CTCAGAGGAG
    GACCTGGATC TCCATGATGA CCGTATGTCC TACCTGTCGG CCATGAGTGC AGATTACCTG
    AGCATGGACA GCCGTCTGAC CAGCGACTAT GAGGACACGG CGGACGAGGG CGGCGCATAC
    ACTGATAATG AGCTTGATGA GTCCACAGAT GAGCCTCAGC CAATGTCTGC CATCAGCCGC
    TCATCTGAAC CCGTCAGTGA GGAGAGGCAC ATGCCTGTTC CCCACGAGCG CTCAAAGAAA
    CCATCGGGCA GAGACGTCCA GCGTGACCCC AGTCCTCCTC CGTCATTTGT CCCAGAACCC
    CCAAAGGTGC GCTCTGCTTC CCGTCCTGCA GACTCCAGGA GTTTTGACTC GCGCTCCAGC
    AGCACCATCA GCAGTGATGC GCCCATCAGC AACAAACCTC TTCCTCCGCC CGTCGCCCAG
    AAGCCCAGTT TCAATACGAG GTCGGCGGAC GACACACCTT CTAATGACCC AGCAGATGAC
    CCTGCCAACC GCACCTTCAG GGGAAAGGTG AAGGCCTTCG AGCAGATGGA CCACCTTGCC
    AGAGCCAAAC GTATGCTGGA GCTGCAGGAG GCCGAACAAG CCCGGCTGGA AATCTCTCAG
    AAACATCCAG ATATTTATGC AGTTCCTAAC AAGCCAAAAC CAAACCAGAA CAGACCACAA
    CCAATTGGCT CCAGCTCAAA CTCCGAGTCC CAGAGCTCCT CCAAAGCAAT GTATTCAGAG
    AGAGGCTCAC ACTACCGGCA CGATGATGAT GACGAAGAGG AGTATCGCCG CCAGCTGGCG
    GACCAGACCC GGAGAGGATA TTACAGCACA CAGAAATACA ATGACACAGA GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188500.1
    CDS310..3906
    Translation

    Target ORF information:

    RefSeq Version NM_001201571.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGAGA CTGTGTGGGA GCAGTACACT GTGACCCTAC AGCGGGACTC CAAGATGGGC 
    TTTGGCCTAG CGGTGTCTGG AGGACGGGAC AATCCAAACG AAGAAAGTGG AGAAATGTCA
    ATCGTCATAT CTGATGTGCT GCAAGGGGGT CCAGCCGATG GCTTGCTGTT TGAAAATGAC
    AGAGTGGTCC AGGTCAACAC TGTGCCGATG GACGGTGTCC CACACTCCTT TGCAGTCCAG
    ACTCTCCGCA AATGTGGCAA AGTTGCCAAA ATTACTGTAA AGCGATGTAG AAAAATCCCT
    GTCAACCTGA CCAAGCGTGC TGCATCCCCT GATGACCGTG TCTTCAGTGG CGACTACAAC
    GATGATTATG ACTATGAACA GGACCAGCAC AGCATCTACA GTGGACGCAG CTACCCTAAC
    CGTGATACTA GTTTAGAGCG TGAGCGCGGC GGCTATGCTG ACGAGCGTGA ACGGGACTAT
    GATCGCAGTC GGGGGAGGAG CATGGAGCGC GGCGTGAGCC CTGATAGACA ATACAGGCGG
    GACGGCAGCC GCGGACGAAT GCTGGACAAC GAGCGCTGCC CCGACCCCCG TTACAGAAGT
    GAAAATACCT TGGATCGCGA CCGCAGTCCA GACCCCCGTT ATCGAAGTGA ACGCACGCTG
    GACCGCAACT ACAGCCCAGA GCGAGGTCGC CGCTTTCGCA GCGAACACAA CCTGGACCGA
    GAGCGAAGTC CAGACACACA ATACCGCAGC GATCGCACAC TGGACAGAAC ACACAGTCCA
    GATACACACT ATAGGCCTGA ACCTGCTCGG GGGTACAGCA GAGAGAATCT CACCAGTGAC
    AATGAGCGCA GGAGGTATGG CTCACATCAG AACGAACCCA TGAAGAAGAG CAACAGCCAA
    GACAGATTGG ATCGTTCGCC TTCACCTGCA CCACTCCGAC ACAATGCCTT GGAAAAGCCA
    CTCAGTGTTA CTCTCCTCAA AAACCGTCCC AATGATGAAT ATGGCCTTCG TCTAGGCAGT
    CAGTTGTTCA TCAAAGAGAT GACGAACACA GGCCTTGCAT CCAGGGAAGG CAAACTACAA
    GAAGGAGACA TTATTCTGAA AATCAACGGT ACGGTCACAG AGAATCTGTC TCTGAGCGAT
    GCAGGGAAGC TGATTGAGAA GTCTCGTGGA AAGCTGCAGC TGGTGGTGCA GAGGGATCGC
    AGTCAAGTGC TCATCCGCAT CCCACCAATG GCAGACAGCG ACTCGGAAAT GGATGACATC
    TCTGAGATCG AGTCGTACCG CTCATATTCT CCTCAGGAGG ATCGGCGGAG TCATCACTCA
    GACCTCTCCT CACACTCCTC CAATGAGAGA TTACAGGACA AAAACAGAGA AGAGCCTCCT
    AAAAGACTGG CTAAAATGGG AGCGATGCCC ACTCCATTTG TATCAGCACC GGTGGATCTG
    CCACCTCCAC CTGTAGAGAA AGAAGAGCCT CGCAGCGAGT CTCCAGCTCC TCCTGTGGTA
    ATTGCTGCTC CAAAAACTGT TCCAGTAAAA GCAAAACCTT TGCCAAAGGT TCCCTTGCTA
    CCCAGTCCAG AAGAGCAGGA AATTTATGGG CCCAACACTG TGATGGTGCG CTTTGTGAAG
    GGTGAAAGCG TTGGTCTGCG TCTGGCTGGA GGAAATGATG TGGGCATTTT CATCGCCGGC
    GTTCAAGAGG ACAGTCCTGC CGAGGTGGAG GGTCTGCGCA CTGGAGACCA AATAGTCAAG
    GTGAATAACA TGGATTTTCG AGGCATGGTC CGTGAAGATG CAGTTCTGTA TTTACTGGAG
    ATTCCCAAAG GAGAGGATGT GACCATTTTG GCTCAAAGCA AACCCGATGT GTATAAAGAC
    ATCCTGGGGT CTGGACGAGG TGATAACTTC TTCATCAGGA CTCATTTTGA ATATGAGAAA
    GAGCTTCCCC AGAGCCTGAA CTTCTCTAGA GGAGAGGTTT TTAAAGTGGT GGACACCCTG
    TATGATGGCA AGCTGGGAAA CTGGCTCGCC ATCCGCATGG ACAAAGACAA TCAGCTCCTG
    GAAAAGGGCA TCATACCCAG CAAGAGCAGG GCGGAGCAGA TGGCAAATGT TCAGCATGCA
    CAGAAAGGTG CTGGTAATGA CAGAGGCGAT TTCTGGAGGC TCAGAGGTCA AAGGGCAGCT
    AAGAAAAAAG ACCTTCGCAA GAGCAGAGAG GATCTCAGTG CAGCTCCAGT CACCACACGC
    TTCCCGGCCT ATGAGAGAGT GGTGCTGCGT GAAGCTGGCT TCAGGCGTCC AGTCGTGATT
    TTTGGCCCCA TTTCAGATGC AGCCAACGAA AAACTTGCCA ATGAACTTCC AAATGAGTTC
    ACCATTGCAA AGACCGAACC AAAAGACGCA GGCTCAGAAA AGTCATCTGG CGTTGTGAGG
    CTAAACACCA TTCGTCAGAT CATCGAACAG GATCTACATG CTCTGCTGGA TGTCACTCCA
    AAGGCTGTAG ACACCCTCAA TTACACTCAG TGGTATCCCA TTGTCATCTT CCTCAACCCA
    GACAGCAAGC AGGGGGTGAA GACCATGAGG AATCGCTTGG TGCCTGGATC CAACCGCAGC
    TCACGCAAAC TTTACGAACA AGCGGTCAAA CTGCGGAAGA CATATGCGCA TCTGTTCACA
    GCTACCATTG ATCTGAACTC TTCTCACGAT GCCTGGTTTG GAAGCCTCAA AGACGCCGTA
    CGAGAACAGC AAGATAAAGC TGTCTGGGTC TGTCAAAGCA AGCTGGATGG CTCAGAGGAG
    GACCTGGATC TCCATGATGA CCGTATGTCC TACCTGTCGG CCATGAGTGC AGATTACCTG
    AGCATGGACA GCCGTCTGAC CAGCGACTAT GAGGACACGG CGGACGAGGG CGGCGCATAC
    ACTGATAATG AGCTTGATGA GTCCACAGAT GAGCCTCAGC CAATGTCTGC CATCAGCCGC
    TCATCTGAAC CCGTCAGTGA GGAGAGGCAC ATGCCTGTTC CCCACGAGCG CTCAAAGAAA
    CCATCGGGCA GAGACGTCCA GCGTGACCCC AGTCCTCCTC CGTCATTTGT CCCAGAACCC
    CCAAAGGTGC GCTCTGCTTC CCGTCCTGCA GACTCCAGGA GTTTTGACTC GCGCTCCAGC
    AGCACCATCA GCAGTGATGC GCCCATCAGC AACAAACCTC TTCCTCCGCC CGTCGCCCAG
    AAGCCCAGTT TCAATACGAG GTCGGCGGAC GACACACCTT CTAATGACCC AGCAGATGAC
    CCTGCCAACC GCACCTTCAG GGGAAAGGTG AAGGCCTTCG AGCAGATGGA CCACCTTGCC
    AGAGCCAAAC GTATGCTGGA GCTGCAGGAG GCCGAACAAG CCCGGCTGGA AATCTCTCAG
    AAACATCCAG ATATTTATGC AGTTCCTAAC AAGCCAAAAC CAAACCAGAA CAGACCACAA
    CCAATTGGCT CCAGCTCAAA CTCCGAGTCC CAGAGCTCCT CCAAAGCAAT GTATTCAGAG
    AGAGGCTCAC ACTACCGGCA CGATGATGAT GACGAAGAGG AGTATCGCCG CCAGCTGGCG
    GACCAGACCC GGAGAGGATA TTACAGCACA CAGAAATACA ATGACACAGA GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15901
    Clone ID Related Accession (Same CDS sequence) XM_005155574.4 , XM_005155574.3
    Accession Version XM_005155574.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155574.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGACGG TCCTCTCGCT GCACAGGAAA TGGGTGCACA CCATGAAGGC CATCCGCATC 
    CTCCAGGGCA TCAACCCAGT CATGGAGGAG ACTGTGTGGG AGCAGTACAC TGTGACCCTA
    CAGCGGGACT CCAAGATGGG CTTTGGCCTA GCGGTGTCTG GAGGACGGGA CAATCCAAAC
    GAAGAAAGTG GAGAAATGTC AATCGTCATA TCTGATGTGC TGCAAGGGGG TCCAGCCGAT
    GGCTTGCTGT TTGAAAATGA CAGAGTGGTC CAGGTCAACA CTGTGCCGAT GGACGGTGTC
    CCACACTCCT TTGCAGTCCA GACTCTCCGC AAATGTGGCA AAGTTGCCAA AATTACTGTA
    AAGCGATGTA GAAAAATCCC TGTCAACCTG ACCAAGCGTG CTGCATCCCC TGATGACCGT
    GTCTTCAGTG GCGACTACAA CGATGATTAT GACTATGAAC AGGACCAGCA CAGCATCTAC
    AGTGGACGCA GCTACCCTAA CCGTGATACT AGTTTAGAGC GTGAGCGCGG CGGCTATGCT
    GACGAGCGTG AACGGGACTA TGATCGCAGT CGGGGGAGGA GCATGGAGCG CGGCGTGAGC
    CCTGATAGAC AATACAGGCG GGACGGCAGC CGCGGACGAA TGCTGGACAA CGAGCGCTGC
    CCCGACCCCC GTTACAGAAG TGAAAATACC TTGGATCGCG ACCGCAGTCC AGACCCCCGT
    TATCGAAGTG AACGCACGCT GGACCGCAAC TACAGCCCAG AGCGAGGTCG CCGCTTTCGC
    AGCGAACACA ACCTGGACCG AGAGCGAAGT CCAGACACAC AATACCGCAG CGATCGCACA
    CTGGACAGAA CACACAGTCC AGATACACAC TATAGGCCTG AACCTGCTCG GGGGTACAGC
    AGAGAGAATC TCACCAGTGA CAATGAGCGC AGGAGGTATG GCTCACATCA GAACGAACCC
    ATGAAGAAGA GCAACAGCCA AGACAGATTG GATCGTTCGC CTTCACCTGC ACCACTCCGA
    CACAATGCCT TGGAAAAGCC ACTCAGTGTT ACTCTCCTCA AAAACCGTCC CAATGATGAA
    TATGGCCTTC GTCTAGGCAG TCAGTTGTTC ATCAAAGAGA TGACGAACAC AGGCCTTGCA
    TCCAGGGAAG GCAAACTACA AGAAGGAGAC ATTATTCTGA AAATCAACGG TACGGTCACA
    GAGAATCTGT CTCTGAGCGA TGCAGGGAAG CTGATTGAGA AGTCTCGTGG AAAGCTGCAG
    CTGGTGGTGC AGAGGGATCG CAGTCAAGTG CTCATCCGCA TCCCACCAAT GGCAGACAGC
    GACTCGGAAA TGGATGACAT CTCTGAGATC GAGTCGTACC GCTCATATTC TCCTCAGGAG
    GATCGGCGGA GTCATCACTC AGACCTCTCC TCACACTCCT CCAATGAGAG ATTACAGGAC
    AAAAACAGAG AAGAGCCTCC TAAAAGACTG GCTAAAATGG GAGCGATGCC CACTCCATTT
    GTATCAGCAC CGGTGGATCT GCCACCTCCA CCTGTAGAGA AAGAAGAGCC TCGCAGCGAG
    TCTCCAGCTC CTCCTGTGGT AATTGCTGCT CCAAAAACTG TTCCAGTAAA AGCAAAACCT
    TTGCCAAAGG TTCCCTTGCT ACCCAGTCCA GAAGAGCAGG AAATTTATGG GCCCAACACT
    GTGATGGTGC GCTTTGTGAA GGGTGAAAGC GTTGGTCTGC GTCTGGCTGG AGGAAATGAT
    GTGGGCATTT TCATCGCCGG CGTTCAAGAG GACAGTCCTG CCGAGGTGGA GGGTCTGCGC
    ACTGGAGACC AAATAGTCAA GGTGAATAAC ATGGATTTTC GAGGCATGGT CCGTGAAGAT
    GCAGTTCTGT ATTTACTGGA GATTCCCAAA GGAGAGGATG TGACCATTTT GGCTCAAAGC
    AAACCCGATG TGTATAAAGA CATCCTGGGG TCTGGACGAG GTGATAACTT CTTCATCAGG
    ACTCATTTTG AATATGAGAA AGAGCTTCCC CAGAGCCTGA ACTTCTCTAG AGGAGAGGTT
    TTTAAAGTGG TGGACACCCT GTATGATGGC AAGCTGGGAA ACTGGCTCGC CATCCGCATG
    GACAAAGACA ATCAGCTCCT GGAAAAGGGC ATCATACCCA GCAAGAGCAG GGCGGAGCAG
    ATGGCAAATG TTCAGCATGC ACAGAAAGGT GCTGGTAATG ACAGAGGCGA TTTCTGGAGG
    CTCAGAGGTC AAAGGGCAGC TAAGAAAAAA GACCTTCGCA AGAGCAGAGA GGATCTCAGT
    GCAGCTCCAG TCACCACACG CTTCCCGGCC TATGAGAGAG TGGTGCTGCG TGAAGCTGGC
    TTCAGGCGTC CAGTCGTGAT TTTTGGCCCC ATTTCAGATG CAGCCAACGA AAAACTTGCC
    AATGAACTTC CAAATGAGTT CACCATTGCA AAGACCGAAC CAAAAGACGC AGGCTCAGAA
    AAGTCATCTG GCGTTGTGAG GCTAAACACC ATTCGTCAGA TCATCGAACA GGATCTACAT
    GCTCTGCTGG ATGTCACTCC AAAGGCTGTA GACACCCTCA ATTACACTCA GTGGTATCCC
    ATTGTCATCT TCCTCAACCC AGACAGCAAG CAGGGGGTGA AGACCATGAG GAATCGCTTG
    GTGCCTGGAT CCAACCGCAG CTCACGCAAA CTTTACGAAC AAGCGGTCAA ACTGCGGAAG
    ACATATGCGC ATCTGTTCAC AGCTACCATT GATCTGAACT CTTCTCACGA TGCCTGGTTT
    GGAAGCCTCA AAGACGCCGT ACGAGAACAG CAAGATAAAG CTGTCTGGGT CTGTCAAAGC
    AAGCTGGATG GCTCAGAGGA GGACCTGGAT CTCCATGATG ACCGTATGTC CTACCTGTCG
    GCCATGAGTG CAGATTACCT GAGCATGGAC AGCCGTCTGA CCAGCGACTA TGAGGACACG
    GCGGACGAGG GCGGCGCATA CACTGATAAT GAGCTTGATG AGTCCACAGA TGAGCCTCAG
    CCAATGTCTG CCATCAGCCG CTCATCTGAA CCCGTCAGTG AGGAGAGGCA CATGCCTGTT
    CCCCACGAGC GCTCAAAGAA ACCATCGGGC AGAGACGTCC AGCGTGACCC CAGTCCTCCT
    CCGTCATTTG TCCCAGAACC CCCAAAGGTG CGCTCTGCTT CCCGTCCTGC AGACTCCAGG
    AGTTTTGACT CGCGCTCCAG CAGCACCATC AGCAGTGATG CGCCCATCAG CAACAAACCT
    CTTCCTCCGC CCGTCGCCCA GAAGCCCAGT TTCAATACGA GGTCGGCGGA CGACACACCT
    TCTAATGACC CAGCAGATGA CCCTGCCAAC CGCACCTTCA GGGGAAAGGT GAAGGCCTTC
    GAGCAGATGG ACCACCTTGC CAGAGCCAAA CGTATGCTGG AGCTGCAGGA GGCCGAACAA
    GCCCGGCTGG AAATCTCTCA GAAACATCCA GATATTTATG CAGTTCCTAA CAAGCCAAAA
    CCAAACCAGA ACAGACCACA ACCAATTGGC TCCAGCTCAA ACTCCGAGTC CCAGAGCTCC
    TCCAAAGCAA TGTATTCAGA GAGAGGCTCA CACTACCGGC ACGATGATGA TGACGAAGAG
    GAGTATCGCC GCCAGCTGGC GGACCAGACC CGGAGAGGAT ATTACAGCAC ACAGAAATAC
    AATGACACAG AGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005155631.1
    CDS323..4000
    Translation

    Target ORF information:

    RefSeq Version XM_005155574.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005155574.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGACGG TCCTCTCGCT GCACAGGAAA TGGGTGCACA CCATGAAGGC CATCCGCATC 
    CTCCAGGGCA TCAACCCAGT CATGGAGGAG ACTGTGTGGG AGCAGTACAC TGTGACCCTA
    CAGCGGGACT CCAAGATGGG CTTTGGCCTA GCGGTGTCTG GAGGACGGGA CAATCCAAAC
    GAAGAAAGTG GAGAAATGTC AATCGTCATA TCTGATGTGC TGCAAGGGGG TCCAGCCGAT
    GGCTTGCTGT TTGAAAATGA CAGAGTGGTC CAGGTCAACA CTGTGCCGAT GGACGGTGTC
    CCACACTCCT TTGCAGTCCA GACTCTCCGC AAATGTGGCA AAGTTGCCAA AATTACTGTA
    AAGCGATGTA GAAAAATCCC TGTCAACCTG ACCAAGCGTG CTGCATCCCC TGATGACCGT
    GTCTTCAGTG GCGACTACAA CGATGATTAT GACTATGAAC AGGACCAGCA CAGCATCTAC
    AGTGGACGCA GCTACCCTAA CCGTGATACT AGTTTAGAGC GTGAGCGCGG CGGCTATGCT
    GACGAGCGTG AACGGGACTA TGATCGCAGT CGGGGGAGGA GCATGGAGCG CGGCGTGAGC
    CCTGATAGAC AATACAGGCG GGACGGCAGC CGCGGACGAA TGCTGGACAA CGAGCGCTGC
    CCCGACCCCC GTTACAGAAG TGAAAATACC TTGGATCGCG ACCGCAGTCC AGACCCCCGT
    TATCGAAGTG AACGCACGCT GGACCGCAAC TACAGCCCAG AGCGAGGTCG CCGCTTTCGC
    AGCGAACACA ACCTGGACCG AGAGCGAAGT CCAGACACAC AATACCGCAG CGATCGCACA
    CTGGACAGAA CACACAGTCC AGATACACAC TATAGGCCTG AACCTGCTCG GGGGTACAGC
    AGAGAGAATC TCACCAGTGA CAATGAGCGC AGGAGGTATG GCTCACATCA GAACGAACCC
    ATGAAGAAGA GCAACAGCCA AGACAGATTG GATCGTTCGC CTTCACCTGC ACCACTCCGA
    CACAATGCCT TGGAAAAGCC ACTCAGTGTT ACTCTCCTCA AAAACCGTCC CAATGATGAA
    TATGGCCTTC GTCTAGGCAG TCAGTTGTTC ATCAAAGAGA TGACGAACAC AGGCCTTGCA
    TCCAGGGAAG GCAAACTACA AGAAGGAGAC ATTATTCTGA AAATCAACGG TACGGTCACA
    GAGAATCTGT CTCTGAGCGA TGCAGGGAAG CTGATTGAGA AGTCTCGTGG AAAGCTGCAG
    CTGGTGGTGC AGAGGGATCG CAGTCAAGTG CTCATCCGCA TCCCACCAAT GGCAGACAGC
    GACTCGGAAA TGGATGACAT CTCTGAGATC GAGTCGTACC GCTCATATTC TCCTCAGGAG
    GATCGGCGGA GTCATCACTC AGACCTCTCC TCACACTCCT CCAATGAGAG ATTACAGGAC
    AAAAACAGAG AAGAGCCTCC TAAAAGACTG GCTAAAATGG GAGCGATGCC CACTCCATTT
    GTATCAGCAC CGGTGGATCT GCCACCTCCA CCTGTAGAGA AAGAAGAGCC TCGCAGCGAG
    TCTCCAGCTC CTCCTGTGGT AATTGCTGCT CCAAAAACTG TTCCAGTAAA AGCAAAACCT
    TTGCCAAAGG TTCCCTTGCT ACCCAGTCCA GAAGAGCAGG AAATTTATGG GCCCAACACT
    GTGATGGTGC GCTTTGTGAA GGGTGAAAGC GTTGGTCTGC GTCTGGCTGG AGGAAATGAT
    GTGGGCATTT TCATCGCCGG CGTTCAAGAG GACAGTCCTG CCGAGGTGGA GGGTCTGCGC
    ACTGGAGACC AAATAGTCAA GGTGAATAAC ATGGATTTTC GAGGCATGGT CCGTGAAGAT
    GCAGTTCTGT ATTTACTGGA GATTCCCAAA GGAGAGGATG TGACCATTTT GGCTCAAAGC
    AAACCCGATG TGTATAAAGA CATCCTGGGG TCTGGACGAG GTGATAACTT CTTCATCAGG
    ACTCATTTTG AATATGAGAA AGAGCTTCCC CAGAGCCTGA ACTTCTCTAG AGGAGAGGTT
    TTTAAAGTGG TGGACACCCT GTATGATGGC AAGCTGGGAA ACTGGCTCGC CATCCGCATG
    GACAAAGACA ATCAGCTCCT GGAAAAGGGC ATCATACCCA GCAAGAGCAG GGCGGAGCAG
    ATGGCAAATG TTCAGCATGC ACAGAAAGGT GCTGGTAATG ACAGAGGCGA TTTCTGGAGG
    CTCAGAGGTC AAAGGGCAGC TAAGAAAAAA GACCTTCGCA AGAGCAGAGA GGATCTCAGT
    GCAGCTCCAG TCACCACACG CTTCCCGGCC TATGAGAGAG TGGTGCTGCG TGAAGCTGGC
    TTCAGGCGTC CAGTCGTGAT TTTTGGCCCC ATTTCAGATG CAGCCAACGA AAAACTTGCC
    AATGAACTTC CAAATGAGTT CACCATTGCA AAGACCGAAC CAAAAGACGC AGGCTCAGAA
    AAGTCATCTG GCGTTGTGAG GCTAAACACC ATTCGTCAGA TCATCGAACA GGATCTACAT
    GCTCTGCTGG ATGTCACTCC AAAGGCTGTA GACACCCTCA ATTACACTCA GTGGTATCCC
    ATTGTCATCT TCCTCAACCC AGACAGCAAG CAGGGGGTGA AGACCATGAG GAATCGCTTG
    GTGCCTGGAT CCAACCGCAG CTCACGCAAA CTTTACGAAC AAGCGGTCAA ACTGCGGAAG
    ACATATGCGC ATCTGTTCAC AGCTACCATT GATCTGAACT CTTCTCACGA TGCCTGGTTT
    GGAAGCCTCA AAGACGCCGT ACGAGAACAG CAAGATAAAG CTGTCTGGGT CTGTCAAAGC
    AAGCTGGATG GCTCAGAGGA GGACCTGGAT CTCCATGATG ACCGTATGTC CTACCTGTCG
    GCCATGAGTG CAGATTACCT GAGCATGGAC AGCCGTCTGA CCAGCGACTA TGAGGACACG
    GCGGACGAGG GCGGCGCATA CACTGATAAT GAGCTTGATG AGTCCACAGA TGAGCCTCAG
    CCAATGTCTG CCATCAGCCG CTCATCTGAA CCCGTCAGTG AGGAGAGGCA CATGCCTGTT
    CCCCACGAGC GCTCAAAGAA ACCATCGGGC AGAGACGTCC AGCGTGACCC CAGTCCTCCT
    CCGTCATTTG TCCCAGAACC CCCAAAGGTG CGCTCTGCTT CCCGTCCTGC AGACTCCAGG
    AGTTTTGACT CGCGCTCCAG CAGCACCATC AGCAGTGATG CGCCCATCAG CAACAAACCT
    CTTCCTCCGC CCGTCGCCCA GAAGCCCAGT TTCAATACGA GGTCGGCGGA CGACACACCT
    TCTAATGACC CAGCAGATGA CCCTGCCAAC CGCACCTTCA GGGGAAAGGT GAAGGCCTTC
    GAGCAGATGG ACCACCTTGC CAGAGCCAAA CGTATGCTGG AGCTGCAGGA GGCCGAACAA
    GCCCGGCTGG AAATCTCTCA GAAACATCCA GATATTTATG CAGTTCCTAA CAAGCCAAAA
    CCAAACCAGA ACAGACCACA ACCAATTGGC TCCAGCTCAA ACTCCGAGTC CCAGAGCTCC
    TCCAAAGCAA TGTATTCAGA GAGAGGCTCA CACTACCGGC ACGATGATGA TGACGAAGAG
    GAGTATCGCC GCCAGCTGGC GGACCAGACC CGGAGAGGAT ATTACAGCAC ACAGAAATAC
    AATGACACAG AGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15901
    Clone ID Related Accession (Same CDS sequence) XM_005155574.4 , XM_005155574.3
    Accession Version XM_005155574.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155574.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGACGG TCCTCTCGCT GCACAGGAAA TGGGTGCACA CCATGAAGGC CATCCGCATC 
    CTCCAGGGCA TCAACCCAGT CATGGAGGAG ACTGTGTGGG AGCAGTACAC TGTGACCCTA
    CAGCGGGACT CCAAGATGGG CTTTGGCCTA GCGGTGTCTG GAGGACGGGA CAATCCAAAC
    GAAGAAAGTG GAGAAATGTC AATCGTCATA TCTGATGTGC TGCAAGGGGG TCCAGCCGAT
    GGCTTGCTGT TTGAAAATGA CAGAGTGGTC CAGGTCAACA CTGTGCCGAT GGACGGTGTC
    CCACACTCCT TTGCAGTCCA GACTCTCCGC AAATGTGGCA AAGTTGCCAA AATTACTGTA
    AAGCGATGTA GAAAAATCCC TGTCAACCTG ACCAAGCGTG CTGCATCCCC TGATGACCGT
    GTCTTCAGTG GCGACTACAA CGATGATTAT GACTATGAAC AGGACCAGCA CAGCATCTAC
    AGTGGACGCA GCTACCCTAA CCGTGATACT AGTTTAGAGC GTGAGCGCGG CGGCTATGCT
    GACGAGCGTG AACGGGACTA TGATCGCAGT CGGGGGAGGA GCATGGAGCG CGGCGTGAGC
    CCTGATAGAC AATACAGGCG GGACGGCAGC CGCGGACGAA TGCTGGACAA CGAGCGCTGC
    CCCGACCCCC GTTACAGAAG TGAAAATACC TTGGATCGCG ACCGCAGTCC AGACCCCCGT
    TATCGAAGTG AACGCACGCT GGACCGCAAC TACAGCCCAG AGCGAGGTCG CCGCTTTCGC
    AGCGAACACA ACCTGGACCG AGAGCGAAGT CCAGACACAC AATACCGCAG CGATCGCACA
    CTGGACAGAA CACACAGTCC AGATACACAC TATAGGCCTG AACCTGCTCG GGGGTACAGC
    AGAGAGAATC TCACCAGTGA CAATGAGCGC AGGAGGTATG GCTCACATCA GAACGAACCC
    ATGAAGAAGA GCAACAGCCA AGACAGATTG GATCGTTCGC CTTCACCTGC ACCACTCCGA
    CACAATGCCT TGGAAAAGCC ACTCAGTGTT ACTCTCCTCA AAAACCGTCC CAATGATGAA
    TATGGCCTTC GTCTAGGCAG TCAGTTGTTC ATCAAAGAGA TGACGAACAC AGGCCTTGCA
    TCCAGGGAAG GCAAACTACA AGAAGGAGAC ATTATTCTGA AAATCAACGG TACGGTCACA
    GAGAATCTGT CTCTGAGCGA TGCAGGGAAG CTGATTGAGA AGTCTCGTGG AAAGCTGCAG
    CTGGTGGTGC AGAGGGATCG CAGTCAAGTG CTCATCCGCA TCCCACCAAT GGCAGACAGC
    GACTCGGAAA TGGATGACAT CTCTGAGATC GAGTCGTACC GCTCATATTC TCCTCAGGAG
    GATCGGCGGA GTCATCACTC AGACCTCTCC TCACACTCCT CCAATGAGAG ATTACAGGAC
    AAAAACAGAG AAGAGCCTCC TAAAAGACTG GCTAAAATGG GAGCGATGCC CACTCCATTT
    GTATCAGCAC CGGTGGATCT GCCACCTCCA CCTGTAGAGA AAGAAGAGCC TCGCAGCGAG
    TCTCCAGCTC CTCCTGTGGT AATTGCTGCT CCAAAAACTG TTCCAGTAAA AGCAAAACCT
    TTGCCAAAGG TTCCCTTGCT ACCCAGTCCA GAAGAGCAGG AAATTTATGG GCCCAACACT
    GTGATGGTGC GCTTTGTGAA GGGTGAAAGC GTTGGTCTGC GTCTGGCTGG AGGAAATGAT
    GTGGGCATTT TCATCGCCGG CGTTCAAGAG GACAGTCCTG CCGAGGTGGA GGGTCTGCGC
    ACTGGAGACC AAATAGTCAA GGTGAATAAC ATGGATTTTC GAGGCATGGT CCGTGAAGAT
    GCAGTTCTGT ATTTACTGGA GATTCCCAAA GGAGAGGATG TGACCATTTT GGCTCAAAGC
    AAACCCGATG TGTATAAAGA CATCCTGGGG TCTGGACGAG GTGATAACTT CTTCATCAGG
    ACTCATTTTG AATATGAGAA AGAGCTTCCC CAGAGCCTGA ACTTCTCTAG AGGAGAGGTT
    TTTAAAGTGG TGGACACCCT GTATGATGGC AAGCTGGGAA ACTGGCTCGC CATCCGCATG
    GACAAAGACA ATCAGCTCCT GGAAAAGGGC ATCATACCCA GCAAGAGCAG GGCGGAGCAG
    ATGGCAAATG TTCAGCATGC ACAGAAAGGT GCTGGTAATG ACAGAGGCGA TTTCTGGAGG
    CTCAGAGGTC AAAGGGCAGC TAAGAAAAAA GACCTTCGCA AGAGCAGAGA GGATCTCAGT
    GCAGCTCCAG TCACCACACG CTTCCCGGCC TATGAGAGAG TGGTGCTGCG TGAAGCTGGC
    TTCAGGCGTC CAGTCGTGAT TTTTGGCCCC ATTTCAGATG CAGCCAACGA AAAACTTGCC
    AATGAACTTC CAAATGAGTT CACCATTGCA AAGACCGAAC CAAAAGACGC AGGCTCAGAA
    AAGTCATCTG GCGTTGTGAG GCTAAACACC ATTCGTCAGA TCATCGAACA GGATCTACAT
    GCTCTGCTGG ATGTCACTCC AAAGGCTGTA GACACCCTCA ATTACACTCA GTGGTATCCC
    ATTGTCATCT TCCTCAACCC AGACAGCAAG CAGGGGGTGA AGACCATGAG GAATCGCTTG
    GTGCCTGGAT CCAACCGCAG CTCACGCAAA CTTTACGAAC AAGCGGTCAA ACTGCGGAAG
    ACATATGCGC ATCTGTTCAC AGCTACCATT GATCTGAACT CTTCTCACGA TGCCTGGTTT
    GGAAGCCTCA AAGACGCCGT ACGAGAACAG CAAGATAAAG CTGTCTGGGT CTGTCAAAGC
    AAGCTGGATG GCTCAGAGGA GGACCTGGAT CTCCATGATG ACCGTATGTC CTACCTGTCG
    GCCATGAGTG CAGATTACCT GAGCATGGAC AGCCGTCTGA CCAGCGACTA TGAGGACACG
    GCGGACGAGG GCGGCGCATA CACTGATAAT GAGCTTGATG AGTCCACAGA TGAGCCTCAG
    CCAATGTCTG CCATCAGCCG CTCATCTGAA CCCGTCAGTG AGGAGAGGCA CATGCCTGTT
    CCCCACGAGC GCTCAAAGAA ACCATCGGGC AGAGACGTCC AGCGTGACCC CAGTCCTCCT
    CCGTCATTTG TCCCAGAACC CCCAAAGGTG CGCTCTGCTT CCCGTCCTGC AGACTCCAGG
    AGTTTTGACT CGCGCTCCAG CAGCACCATC AGCAGTGATG CGCCCATCAG CAACAAACCT
    CTTCCTCCGC CCGTCGCCCA GAAGCCCAGT TTCAATACGA GGTCGGCGGA CGACACACCT
    TCTAATGACC CAGCAGATGA CCCTGCCAAC CGCACCTTCA GGGGAAAGGT GAAGGCCTTC
    GAGCAGATGG ACCACCTTGC CAGAGCCAAA CGTATGCTGG AGCTGCAGGA GGCCGAACAA
    GCCCGGCTGG AAATCTCTCA GAAACATCCA GATATTTATG CAGTTCCTAA CAAGCCAAAA
    CCAAACCAGA ACAGACCACA ACCAATTGGC TCCAGCTCAA ACTCCGAGTC CCAGAGCTCC
    TCCAAAGCAA TGTATTCAGA GAGAGGCTCA CACTACCGGC ACGATGATGA TGACGAAGAG
    GAGTATCGCC GCCAGCTGGC GGACCAGACC CGGAGAGGAT ATTACAGCAC ACAGAAATAC
    AATGACACAG AGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005155631.1
    CDS320..3997
    Translation

    Target ORF information:

    RefSeq Version XM_005155574.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005155574.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGACGG TCCTCTCGCT GCACAGGAAA TGGGTGCACA CCATGAAGGC CATCCGCATC 
    CTCCAGGGCA TCAACCCAGT CATGGAGGAG ACTGTGTGGG AGCAGTACAC TGTGACCCTA
    CAGCGGGACT CCAAGATGGG CTTTGGCCTA GCGGTGTCTG GAGGACGGGA CAATCCAAAC
    GAAGAAAGTG GAGAAATGTC AATCGTCATA TCTGATGTGC TGCAAGGGGG TCCAGCCGAT
    GGCTTGCTGT TTGAAAATGA CAGAGTGGTC CAGGTCAACA CTGTGCCGAT GGACGGTGTC
    CCACACTCCT TTGCAGTCCA GACTCTCCGC AAATGTGGCA AAGTTGCCAA AATTACTGTA
    AAGCGATGTA GAAAAATCCC TGTCAACCTG ACCAAGCGTG CTGCATCCCC TGATGACCGT
    GTCTTCAGTG GCGACTACAA CGATGATTAT GACTATGAAC AGGACCAGCA CAGCATCTAC
    AGTGGACGCA GCTACCCTAA CCGTGATACT AGTTTAGAGC GTGAGCGCGG CGGCTATGCT
    GACGAGCGTG AACGGGACTA TGATCGCAGT CGGGGGAGGA GCATGGAGCG CGGCGTGAGC
    CCTGATAGAC AATACAGGCG GGACGGCAGC CGCGGACGAA TGCTGGACAA CGAGCGCTGC
    CCCGACCCCC GTTACAGAAG TGAAAATACC TTGGATCGCG ACCGCAGTCC AGACCCCCGT
    TATCGAAGTG AACGCACGCT GGACCGCAAC TACAGCCCAG AGCGAGGTCG CCGCTTTCGC
    AGCGAACACA ACCTGGACCG AGAGCGAAGT CCAGACACAC AATACCGCAG CGATCGCACA
    CTGGACAGAA CACACAGTCC AGATACACAC TATAGGCCTG AACCTGCTCG GGGGTACAGC
    AGAGAGAATC TCACCAGTGA CAATGAGCGC AGGAGGTATG GCTCACATCA GAACGAACCC
    ATGAAGAAGA GCAACAGCCA AGACAGATTG GATCGTTCGC CTTCACCTGC ACCACTCCGA
    CACAATGCCT TGGAAAAGCC ACTCAGTGTT ACTCTCCTCA AAAACCGTCC CAATGATGAA
    TATGGCCTTC GTCTAGGCAG TCAGTTGTTC ATCAAAGAGA TGACGAACAC AGGCCTTGCA
    TCCAGGGAAG GCAAACTACA AGAAGGAGAC ATTATTCTGA AAATCAACGG TACGGTCACA
    GAGAATCTGT CTCTGAGCGA TGCAGGGAAG CTGATTGAGA AGTCTCGTGG AAAGCTGCAG
    CTGGTGGTGC AGAGGGATCG CAGTCAAGTG CTCATCCGCA TCCCACCAAT GGCAGACAGC
    GACTCGGAAA TGGATGACAT CTCTGAGATC GAGTCGTACC GCTCATATTC TCCTCAGGAG
    GATCGGCGGA GTCATCACTC AGACCTCTCC TCACACTCCT CCAATGAGAG ATTACAGGAC
    AAAAACAGAG AAGAGCCTCC TAAAAGACTG GCTAAAATGG GAGCGATGCC CACTCCATTT
    GTATCAGCAC CGGTGGATCT GCCACCTCCA CCTGTAGAGA AAGAAGAGCC TCGCAGCGAG
    TCTCCAGCTC CTCCTGTGGT AATTGCTGCT CCAAAAACTG TTCCAGTAAA AGCAAAACCT
    TTGCCAAAGG TTCCCTTGCT ACCCAGTCCA GAAGAGCAGG AAATTTATGG GCCCAACACT
    GTGATGGTGC GCTTTGTGAA GGGTGAAAGC GTTGGTCTGC GTCTGGCTGG AGGAAATGAT
    GTGGGCATTT TCATCGCCGG CGTTCAAGAG GACAGTCCTG CCGAGGTGGA GGGTCTGCGC
    ACTGGAGACC AAATAGTCAA GGTGAATAAC ATGGATTTTC GAGGCATGGT CCGTGAAGAT
    GCAGTTCTGT ATTTACTGGA GATTCCCAAA GGAGAGGATG TGACCATTTT GGCTCAAAGC
    AAACCCGATG TGTATAAAGA CATCCTGGGG TCTGGACGAG GTGATAACTT CTTCATCAGG
    ACTCATTTTG AATATGAGAA AGAGCTTCCC CAGAGCCTGA ACTTCTCTAG AGGAGAGGTT
    TTTAAAGTGG TGGACACCCT GTATGATGGC AAGCTGGGAA ACTGGCTCGC CATCCGCATG
    GACAAAGACA ATCAGCTCCT GGAAAAGGGC ATCATACCCA GCAAGAGCAG GGCGGAGCAG
    ATGGCAAATG TTCAGCATGC ACAGAAAGGT GCTGGTAATG ACAGAGGCGA TTTCTGGAGG
    CTCAGAGGTC AAAGGGCAGC TAAGAAAAAA GACCTTCGCA AGAGCAGAGA GGATCTCAGT
    GCAGCTCCAG TCACCACACG CTTCCCGGCC TATGAGAGAG TGGTGCTGCG TGAAGCTGGC
    TTCAGGCGTC CAGTCGTGAT TTTTGGCCCC ATTTCAGATG CAGCCAACGA AAAACTTGCC
    AATGAACTTC CAAATGAGTT CACCATTGCA AAGACCGAAC CAAAAGACGC AGGCTCAGAA
    AAGTCATCTG GCGTTGTGAG GCTAAACACC ATTCGTCAGA TCATCGAACA GGATCTACAT
    GCTCTGCTGG ATGTCACTCC AAAGGCTGTA GACACCCTCA ATTACACTCA GTGGTATCCC
    ATTGTCATCT TCCTCAACCC AGACAGCAAG CAGGGGGTGA AGACCATGAG GAATCGCTTG
    GTGCCTGGAT CCAACCGCAG CTCACGCAAA CTTTACGAAC AAGCGGTCAA ACTGCGGAAG
    ACATATGCGC ATCTGTTCAC AGCTACCATT GATCTGAACT CTTCTCACGA TGCCTGGTTT
    GGAAGCCTCA AAGACGCCGT ACGAGAACAG CAAGATAAAG CTGTCTGGGT CTGTCAAAGC
    AAGCTGGATG GCTCAGAGGA GGACCTGGAT CTCCATGATG ACCGTATGTC CTACCTGTCG
    GCCATGAGTG CAGATTACCT GAGCATGGAC AGCCGTCTGA CCAGCGACTA TGAGGACACG
    GCGGACGAGG GCGGCGCATA CACTGATAAT GAGCTTGATG AGTCCACAGA TGAGCCTCAG
    CCAATGTCTG CCATCAGCCG CTCATCTGAA CCCGTCAGTG AGGAGAGGCA CATGCCTGTT
    CCCCACGAGC GCTCAAAGAA ACCATCGGGC AGAGACGTCC AGCGTGACCC CAGTCCTCCT
    CCGTCATTTG TCCCAGAACC CCCAAAGGTG CGCTCTGCTT CCCGTCCTGC AGACTCCAGG
    AGTTTTGACT CGCGCTCCAG CAGCACCATC AGCAGTGATG CGCCCATCAG CAACAAACCT
    CTTCCTCCGC CCGTCGCCCA GAAGCCCAGT TTCAATACGA GGTCGGCGGA CGACACACCT
    TCTAATGACC CAGCAGATGA CCCTGCCAAC CGCACCTTCA GGGGAAAGGT GAAGGCCTTC
    GAGCAGATGG ACCACCTTGC CAGAGCCAAA CGTATGCTGG AGCTGCAGGA GGCCGAACAA
    GCCCGGCTGG AAATCTCTCA GAAACATCCA GATATTTATG CAGTTCCTAA CAAGCCAAAA
    CCAAACCAGA ACAGACCACA ACCAATTGGC TCCAGCTCAA ACTCCGAGTC CCAGAGCTCC
    TCCAAAGCAA TGTATTCAGA GAGAGGCTCA CACTACCGGC ACGATGATGA TGACGAAGAG
    GAGTATCGCC GCCAGCTGGC GGACCAGACC CGGAGAGGAT ATTACAGCAC ACAGAAATAC
    AATGACACAG AGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44613
    Clone ID Related Accession (Same CDS sequence) XM_009305192.2 , XM_009305192.3
    Accession Version XM_009305192.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGTACA AGAAATTCAT CACCATCATG CAAGCGGCCA TCGGGGTCGC ACCGCTCAAC 
    AAACGAGAGC TGATGCCACC CGGCCGGAGG TTGTGGAGAC CCTCAGGTGA CCAGACCAAC
    CAAATTGAGT TGTTCCGCTC AAGCCGTTCC AACAAGAAAA GGTTTTCCTT GACCTACGAA
    GCTCAATATC GGTGTCTGCG ACGCCTCTCT ACTCCAAGCT TCCCGGCCCT TTTACAGAAC
    CCAGTCATGG AGGAGACTGT GTGGGAGCAG TACACTGTGA CCCTACAGCG GGACTCCAAG
    ATGGGCTTTG GCCTAGCGGT GTCTGGAGGA CGGGACAATC CAAACGAAGA AAGTGGAGAA
    ATGTCAATCG TCATATCTGA TGTGCTGCAA GGGGGTCCAG CCGATGGCTT GCTGTTTGAA
    AATGACAGAG TGGTCCAGGT CAACACTGTG CCGATGGACG GTGTCCCACA CTCCTTTGCA
    GTCCAGACTC TCCGCAAATG TGGCAAAGTT GCCAAAATTA CTGTAAAGCG ATGTAGAAAA
    ATCCCTGTCA ACCTGACCAA GCGTGCTGCA TCCCCTGATG ACCGTGTCTT CAGTGGCGAC
    TACAACGATG ATTATGACTA TGAACAGGAC CAGCACAGCA TCTACAGTGG ACGCAGCTAC
    CCTAACCGTG ATACTAGTTT AGAGCGTGAG CGCGGCGGCT ATGCTGACGA GCGTGAACGG
    GACTATGATC GCAGTCGGGG GAGGAGCATG GAGCGCGGCG TGAGCCCTGA TAGACAATAC
    AGGCGGGACG GCAGCCGCGG ACGAATGCTG GACAACGAGC GCTGCCCCGA CCCCCGTTAC
    AGAAGTGAAA ATACCTTGGA TCGCGACCGC AGTCCAGACC CCCGTTATCG AAGTGAACGC
    ACGCTGGACC GCAACTACAG CCCAGAGCGA GGTCGCCGCT TTCGCAGCGA ACACAACCTG
    GACCGAGAGC GAAGTCCAGA CACACAATAC CGCAGCGATC GCACACTGGA CAGAACACAC
    AGTCCAGATA CACACTATAG GCCTGAACCT GCTCGGGGGT ACAGCAGAGA GAATCTCACC
    AGTGACAATG AGCGCAGGAG GTATGGCTCA CATCAGAACG AACCCATGAA GAAGAGCAAC
    AGCCAAGACA GATTGGATCG TTCGCCTTCA CCTGCACCAC TCCGACACAA TGCCTTGGAA
    AAGCCACTCA GTGTTACTCT CCTCAAAAAC CGTCCCAATG ATGAATATGG CCTTCGTCTA
    GGCAGTCAGT TGTTCATCAA AGAGATGACG AACACAGGCC TTGCATCCAG GGAAGGCAAA
    CTACAAGAAG GAGACATTAT TCTGAAAATC AACGGTACGG TCACAGAGAA TCTGTCTCTG
    AGCGATGCAG GGAAGCTGAT TGAGAAGTCT CGTGGAAAGC TGCAGCTGGT GGTGCAGAGG
    GATCGCAGTC AAGTGCTCAT CCGCATCCCA CCAATGGCAG ACAGCGACTC GGAAATGGAT
    GACATCTCTG AGATCGAGTC GTACCGCTCA TATTCTCCTC AGGAGGATCG GCGGAGTCAT
    CACTCAGACC TCTCCTCACA CTCCTCCAAT GAGAGATTAC AGGACAAAAA CAGAGAAGAG
    CCTCCTAAAA GACTGGCTAA AATGGGAGCG ATGCCCACTC CATTTGTATC AGCACCGGTG
    GATCTGCCAC CTCCACCTGT AGAGAAAGAA GAGCCTCGCA GCGAGTCTCC AGCTCCTCCT
    GTGGTAATTG CTGCTCCAAA AACTGTTCCA GTAAAAGCAA AACCTTTGCC AAAGGTTCCC
    TTGCTACCCA GTCCAGAAGA GCAGGAAATT TATGGGCCCA ACACTGTGAT GGTGCGCTTT
    GTGAAGGGTG AAAGCGTTGG TCTGCGTCTG GCTGGAGGAA ATGATGTGGG CATTTTCATC
    GCCGGCGTTC AAGAGGACAG TCCTGCCGAG GTGGAGGGTC TGCGCACTGG AGACCAAATA
    GTCAAGGTGA ATAACATGGA TTTTCGAGGC ATGGTCCGTG AAGATGCAGT TCTGTATTTA
    CTGGAGATTC CCAAAGGAGA GGATGTGACC ATTTTGGCTC AAAGCAAACC CGATGTGTAT
    AAAGACATCC TGGGGTCTGG ACGAGGTGAT AACTTCTTCA TCAGGACTCA TTTTGAATAT
    GAGAAAGAGC TTCCCCAGAG CCTGAACTTC TCTAGAGGAG AGGTTTTTAA AGTGGTGGAC
    ACCCTGTATG ATGGCAAGCT GGGAAACTGG CTCGCCATCC GCATGGACAA AGACAATCAG
    CTCCTGGAAA AGGGCATCAT ACCCAGCAAG AGCAGGGCGG AGCAGATGGC AAATGTTCAG
    CATGCACAGA AAGGTGCTGG TAATGACAGA GGCGATTTCT GGAGGCTCAG AGGTCAAAGG
    GCAGCTAAGA AAAAAGACCT TCGCAAGAGC AGAGAGGATC TCAGTGCAGC TCCAGTCACC
    ACACGCTTCC CGGCCTATGA GAGAGTGGTG CTGCGTGAAG CTGGCTTCAG GCGTCCAGTC
    GTGATTTTTG GCCCCATTTC AGATGCAGCC AACGAAAAAC TTGCCAATGA ACTTCCAAAT
    GAGTTCACCA TTGCAAAGAC CGAACCAAAA GACGCAGGCT CAGAAAAGTC ATCTGGCGTT
    GTGAGGCTAA ACACCATTCG TCAGATCATC GAACAGGATC TACATGCTCT GCTGGATGTC
    ACTCCAAAGG CTGTAGACAC CCTCAATTAC ACTCAGTGGT ATCCCATTGT CATCTTCCTC
    AACCCAGACA GCAAGCAGGG GGTGAAGACC ATGAGGAATC GCTTGGTGCC TGGATCCAAC
    CGCAGCTCAC GCAAACTTTA CGAACAAGCG GTCAAACTGC GGAAGACATA TGCGCATCTG
    TTCACAGCTA CCATTGATCT GAACTCTTCT CACGATGCCT GGTTTGGAAG CCTCAAAGAC
    GCCGTACGAG AACAGCAAGA TAAAGCTGTC TGGGTCTGTC AAAGCAAGCT GGATGGCTCA
    GAGGAGGACC TGGATCTCCA TGATGACCGT ATGTCCTACC TGTCGGCCAT GAGTGCAGAT
    TACCTGAGCA TGGACAGCCG TCTGACCAGC GACTATGAGG ACACGGCGGA CGAGGGCGGC
    GCATACACTG ATAATGAGCT TGATGAGTCC ACAGATGAGC CTCAGCCAAT GTCTGCCATC
    AGCCGCTCAT CTGAACCCGT CAGTGAGGAG AGGCACATGC CTGTTCCCCA CGAGCGCTCA
    AAGAAACCAT CGGGCAGAGA CGTCCAGCGT GACCCCAGTC CTCCTCCGTC ATTTGTCCCA
    GAACCCCCAA AGGTGCGCTC TGCTTCCCGT CCTGCAGACT CCAGGAGTTT TGACTCGCGC
    TCCAGCAGCA CCATCAGCAG TGATGCGCCC ATCAGCAACA AACCTCTTCC TCCGCCCGTC
    GCCCAGAAGC CCAGTTTCAA TACGAGGTCG GCGGACGACA CACCTTCTAA TGACCCAGCA
    GATGACCCTG CCAACCGCAC CTTCAGGGGA AAGGTGAAGG CCTTCGAGCA GATGGACCAC
    CTTGCCAGAG CCAAACGTAT GCTGGAGCTG CAGGAGGCCG AACAAGCCCG GCTGGAAATC
    TCTCAGAAAC ATCCAGATAT TTATGCAGTT CCTAACAAGC CAAAACCAAA CCAGAACAGA
    CCACAACCAA TTGGCTCCAG CTCAAACTCC GAGTCCCAGA GCTCCTCCAA AGCAATGTAT
    TCAGAGAGAG GCTCACACTA CCGGCACGAT GATGATGACG AAGAGGAGTA TCGCCGCCAG
    CTGGCGGACC AGACCCGGAG AGGATATTAC AGCACACAGA AATACAATGA CACAGAGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303467.1
    CDS266..4108
    Translation

    Target ORF information:

    RefSeq Version XM_009305192.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305192.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGTACA AGAAATTCAT CACCATCATG CAAGCGGCCA TCGGGGTCGC ACCGCTCAAC 
    AAACGAGAGC TGATGCCACC CGGCCGGAGG TTGTGGAGAC CCTCAGGTGA CCAGACCAAC
    CAAATTGAGT TGTTCCGCTC AAGCCGTTCC AACAAGAAAA GGTTTTCCTT GACCTACGAA
    GCTCAATATC GGTGTCTGCG ACGCCTCTCT ACTCCAAGCT TCCCGGCCCT TTTACAGAAC
    CCAGTCATGG AGGAGACTGT GTGGGAGCAG TACACTGTGA CCCTACAGCG GGACTCCAAG
    ATGGGCTTTG GCCTAGCGGT GTCTGGAGGA CGGGACAATC CAAACGAAGA AAGTGGAGAA
    ATGTCAATCG TCATATCTGA TGTGCTGCAA GGGGGTCCAG CCGATGGCTT GCTGTTTGAA
    AATGACAGAG TGGTCCAGGT CAACACTGTG CCGATGGACG GTGTCCCACA CTCCTTTGCA
    GTCCAGACTC TCCGCAAATG TGGCAAAGTT GCCAAAATTA CTGTAAAGCG ATGTAGAAAA
    ATCCCTGTCA ACCTGACCAA GCGTGCTGCA TCCCCTGATG ACCGTGTCTT CAGTGGCGAC
    TACAACGATG ATTATGACTA TGAACAGGAC CAGCACAGCA TCTACAGTGG ACGCAGCTAC
    CCTAACCGTG ATACTAGTTT AGAGCGTGAG CGCGGCGGCT ATGCTGACGA GCGTGAACGG
    GACTATGATC GCAGTCGGGG GAGGAGCATG GAGCGCGGCG TGAGCCCTGA TAGACAATAC
    AGGCGGGACG GCAGCCGCGG ACGAATGCTG GACAACGAGC GCTGCCCCGA CCCCCGTTAC
    AGAAGTGAAA ATACCTTGGA TCGCGACCGC AGTCCAGACC CCCGTTATCG AAGTGAACGC
    ACGCTGGACC GCAACTACAG CCCAGAGCGA GGTCGCCGCT TTCGCAGCGA ACACAACCTG
    GACCGAGAGC GAAGTCCAGA CACACAATAC CGCAGCGATC GCACACTGGA CAGAACACAC
    AGTCCAGATA CACACTATAG GCCTGAACCT GCTCGGGGGT ACAGCAGAGA GAATCTCACC
    AGTGACAATG AGCGCAGGAG GTATGGCTCA CATCAGAACG AACCCATGAA GAAGAGCAAC
    AGCCAAGACA GATTGGATCG TTCGCCTTCA CCTGCACCAC TCCGACACAA TGCCTTGGAA
    AAGCCACTCA GTGTTACTCT CCTCAAAAAC CGTCCCAATG ATGAATATGG CCTTCGTCTA
    GGCAGTCAGT TGTTCATCAA AGAGATGACG AACACAGGCC TTGCATCCAG GGAAGGCAAA
    CTACAAGAAG GAGACATTAT TCTGAAAATC AACGGTACGG TCACAGAGAA TCTGTCTCTG
    AGCGATGCAG GGAAGCTGAT TGAGAAGTCT CGTGGAAAGC TGCAGCTGGT GGTGCAGAGG
    GATCGCAGTC AAGTGCTCAT CCGCATCCCA CCAATGGCAG ACAGCGACTC GGAAATGGAT
    GACATCTCTG AGATCGAGTC GTACCGCTCA TATTCTCCTC AGGAGGATCG GCGGAGTCAT
    CACTCAGACC TCTCCTCACA CTCCTCCAAT GAGAGATTAC AGGACAAAAA CAGAGAAGAG
    CCTCCTAAAA GACTGGCTAA AATGGGAGCG ATGCCCACTC CATTTGTATC AGCACCGGTG
    GATCTGCCAC CTCCACCTGT AGAGAAAGAA GAGCCTCGCA GCGAGTCTCC AGCTCCTCCT
    GTGGTAATTG CTGCTCCAAA AACTGTTCCA GTAAAAGCAA AACCTTTGCC AAAGGTTCCC
    TTGCTACCCA GTCCAGAAGA GCAGGAAATT TATGGGCCCA ACACTGTGAT GGTGCGCTTT
    GTGAAGGGTG AAAGCGTTGG TCTGCGTCTG GCTGGAGGAA ATGATGTGGG CATTTTCATC
    GCCGGCGTTC AAGAGGACAG TCCTGCCGAG GTGGAGGGTC TGCGCACTGG AGACCAAATA
    GTCAAGGTGA ATAACATGGA TTTTCGAGGC ATGGTCCGTG AAGATGCAGT TCTGTATTTA
    CTGGAGATTC CCAAAGGAGA GGATGTGACC ATTTTGGCTC AAAGCAAACC CGATGTGTAT
    AAAGACATCC TGGGGTCTGG ACGAGGTGAT AACTTCTTCA TCAGGACTCA TTTTGAATAT
    GAGAAAGAGC TTCCCCAGAG CCTGAACTTC TCTAGAGGAG AGGTTTTTAA AGTGGTGGAC
    ACCCTGTATG ATGGCAAGCT GGGAAACTGG CTCGCCATCC GCATGGACAA AGACAATCAG
    CTCCTGGAAA AGGGCATCAT ACCCAGCAAG AGCAGGGCGG AGCAGATGGC AAATGTTCAG
    CATGCACAGA AAGGTGCTGG TAATGACAGA GGCGATTTCT GGAGGCTCAG AGGTCAAAGG
    GCAGCTAAGA AAAAAGACCT TCGCAAGAGC AGAGAGGATC TCAGTGCAGC TCCAGTCACC
    ACACGCTTCC CGGCCTATGA GAGAGTGGTG CTGCGTGAAG CTGGCTTCAG GCGTCCAGTC
    GTGATTTTTG GCCCCATTTC AGATGCAGCC AACGAAAAAC TTGCCAATGA ACTTCCAAAT
    GAGTTCACCA TTGCAAAGAC CGAACCAAAA GACGCAGGCT CAGAAAAGTC ATCTGGCGTT
    GTGAGGCTAA ACACCATTCG TCAGATCATC GAACAGGATC TACATGCTCT GCTGGATGTC
    ACTCCAAAGG CTGTAGACAC CCTCAATTAC ACTCAGTGGT ATCCCATTGT CATCTTCCTC
    AACCCAGACA GCAAGCAGGG GGTGAAGACC ATGAGGAATC GCTTGGTGCC TGGATCCAAC
    CGCAGCTCAC GCAAACTTTA CGAACAAGCG GTCAAACTGC GGAAGACATA TGCGCATCTG
    TTCACAGCTA CCATTGATCT GAACTCTTCT CACGATGCCT GGTTTGGAAG CCTCAAAGAC
    GCCGTACGAG AACAGCAAGA TAAAGCTGTC TGGGTCTGTC AAAGCAAGCT GGATGGCTCA
    GAGGAGGACC TGGATCTCCA TGATGACCGT ATGTCCTACC TGTCGGCCAT GAGTGCAGAT
    TACCTGAGCA TGGACAGCCG TCTGACCAGC GACTATGAGG ACACGGCGGA CGAGGGCGGC
    GCATACACTG ATAATGAGCT TGATGAGTCC ACAGATGAGC CTCAGCCAAT GTCTGCCATC
    AGCCGCTCAT CTGAACCCGT CAGTGAGGAG AGGCACATGC CTGTTCCCCA CGAGCGCTCA
    AAGAAACCAT CGGGCAGAGA CGTCCAGCGT GACCCCAGTC CTCCTCCGTC ATTTGTCCCA
    GAACCCCCAA AGGTGCGCTC TGCTTCCCGT CCTGCAGACT CCAGGAGTTT TGACTCGCGC
    TCCAGCAGCA CCATCAGCAG TGATGCGCCC ATCAGCAACA AACCTCTTCC TCCGCCCGTC
    GCCCAGAAGC CCAGTTTCAA TACGAGGTCG GCGGACGACA CACCTTCTAA TGACCCAGCA
    GATGACCCTG CCAACCGCAC CTTCAGGGGA AAGGTGAAGG CCTTCGAGCA GATGGACCAC
    CTTGCCAGAG CCAAACGTAT GCTGGAGCTG CAGGAGGCCG AACAAGCCCG GCTGGAAATC
    TCTCAGAAAC ATCCAGATAT TTATGCAGTT CCTAACAAGC CAAAACCAAA CCAGAACAGA
    CCACAACCAA TTGGCTCCAG CTCAAACTCC GAGTCCCAGA GCTCCTCCAA AGCAATGTAT
    TCAGAGAGAG GCTCACACTA CCGGCACGAT GATGATGACG AAGAGGAGTA TCGCCGCCAG
    CTGGCGGACC AGACCCGGAG AGGATATTAC AGCACACAGA AATACAATGA CACAGAGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44613
    Clone ID Related Accession (Same CDS sequence) XM_009305192.2 , XM_009305192.3
    Accession Version XM_009305192.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGTACA AGAAATTCAT CACCATCATG CAAGCGGCCA TCGGGGTCGC ACCGCTCAAC 
    AAACGAGAGC TGATGCCACC CGGCCGGAGG TTGTGGAGAC CCTCAGGTGA CCAGACCAAC
    CAAATTGAGT TGTTCCGCTC AAGCCGTTCC AACAAGAAAA GGTTTTCCTT GACCTACGAA
    GCTCAATATC GGTGTCTGCG ACGCCTCTCT ACTCCAAGCT TCCCGGCCCT TTTACAGAAC
    CCAGTCATGG AGGAGACTGT GTGGGAGCAG TACACTGTGA CCCTACAGCG GGACTCCAAG
    ATGGGCTTTG GCCTAGCGGT GTCTGGAGGA CGGGACAATC CAAACGAAGA AAGTGGAGAA
    ATGTCAATCG TCATATCTGA TGTGCTGCAA GGGGGTCCAG CCGATGGCTT GCTGTTTGAA
    AATGACAGAG TGGTCCAGGT CAACACTGTG CCGATGGACG GTGTCCCACA CTCCTTTGCA
    GTCCAGACTC TCCGCAAATG TGGCAAAGTT GCCAAAATTA CTGTAAAGCG ATGTAGAAAA
    ATCCCTGTCA ACCTGACCAA GCGTGCTGCA TCCCCTGATG ACCGTGTCTT CAGTGGCGAC
    TACAACGATG ATTATGACTA TGAACAGGAC CAGCACAGCA TCTACAGTGG ACGCAGCTAC
    CCTAACCGTG ATACTAGTTT AGAGCGTGAG CGCGGCGGCT ATGCTGACGA GCGTGAACGG
    GACTATGATC GCAGTCGGGG GAGGAGCATG GAGCGCGGCG TGAGCCCTGA TAGACAATAC
    AGGCGGGACG GCAGCCGCGG ACGAATGCTG GACAACGAGC GCTGCCCCGA CCCCCGTTAC
    AGAAGTGAAA ATACCTTGGA TCGCGACCGC AGTCCAGACC CCCGTTATCG AAGTGAACGC
    ACGCTGGACC GCAACTACAG CCCAGAGCGA GGTCGCCGCT TTCGCAGCGA ACACAACCTG
    GACCGAGAGC GAAGTCCAGA CACACAATAC CGCAGCGATC GCACACTGGA CAGAACACAC
    AGTCCAGATA CACACTATAG GCCTGAACCT GCTCGGGGGT ACAGCAGAGA GAATCTCACC
    AGTGACAATG AGCGCAGGAG GTATGGCTCA CATCAGAACG AACCCATGAA GAAGAGCAAC
    AGCCAAGACA GATTGGATCG TTCGCCTTCA CCTGCACCAC TCCGACACAA TGCCTTGGAA
    AAGCCACTCA GTGTTACTCT CCTCAAAAAC CGTCCCAATG ATGAATATGG CCTTCGTCTA
    GGCAGTCAGT TGTTCATCAA AGAGATGACG AACACAGGCC TTGCATCCAG GGAAGGCAAA
    CTACAAGAAG GAGACATTAT TCTGAAAATC AACGGTACGG TCACAGAGAA TCTGTCTCTG
    AGCGATGCAG GGAAGCTGAT TGAGAAGTCT CGTGGAAAGC TGCAGCTGGT GGTGCAGAGG
    GATCGCAGTC AAGTGCTCAT CCGCATCCCA CCAATGGCAG ACAGCGACTC GGAAATGGAT
    GACATCTCTG AGATCGAGTC GTACCGCTCA TATTCTCCTC AGGAGGATCG GCGGAGTCAT
    CACTCAGACC TCTCCTCACA CTCCTCCAAT GAGAGATTAC AGGACAAAAA CAGAGAAGAG
    CCTCCTAAAA GACTGGCTAA AATGGGAGCG ATGCCCACTC CATTTGTATC AGCACCGGTG
    GATCTGCCAC CTCCACCTGT AGAGAAAGAA GAGCCTCGCA GCGAGTCTCC AGCTCCTCCT
    GTGGTAATTG CTGCTCCAAA AACTGTTCCA GTAAAAGCAA AACCTTTGCC AAAGGTTCCC
    TTGCTACCCA GTCCAGAAGA GCAGGAAATT TATGGGCCCA ACACTGTGAT GGTGCGCTTT
    GTGAAGGGTG AAAGCGTTGG TCTGCGTCTG GCTGGAGGAA ATGATGTGGG CATTTTCATC
    GCCGGCGTTC AAGAGGACAG TCCTGCCGAG GTGGAGGGTC TGCGCACTGG AGACCAAATA
    GTCAAGGTGA ATAACATGGA TTTTCGAGGC ATGGTCCGTG AAGATGCAGT TCTGTATTTA
    CTGGAGATTC CCAAAGGAGA GGATGTGACC ATTTTGGCTC AAAGCAAACC CGATGTGTAT
    AAAGACATCC TGGGGTCTGG ACGAGGTGAT AACTTCTTCA TCAGGACTCA TTTTGAATAT
    GAGAAAGAGC TTCCCCAGAG CCTGAACTTC TCTAGAGGAG AGGTTTTTAA AGTGGTGGAC
    ACCCTGTATG ATGGCAAGCT GGGAAACTGG CTCGCCATCC GCATGGACAA AGACAATCAG
    CTCCTGGAAA AGGGCATCAT ACCCAGCAAG AGCAGGGCGG AGCAGATGGC AAATGTTCAG
    CATGCACAGA AAGGTGCTGG TAATGACAGA GGCGATTTCT GGAGGCTCAG AGGTCAAAGG
    GCAGCTAAGA AAAAAGACCT TCGCAAGAGC AGAGAGGATC TCAGTGCAGC TCCAGTCACC
    ACACGCTTCC CGGCCTATGA GAGAGTGGTG CTGCGTGAAG CTGGCTTCAG GCGTCCAGTC
    GTGATTTTTG GCCCCATTTC AGATGCAGCC AACGAAAAAC TTGCCAATGA ACTTCCAAAT
    GAGTTCACCA TTGCAAAGAC CGAACCAAAA GACGCAGGCT CAGAAAAGTC ATCTGGCGTT
    GTGAGGCTAA ACACCATTCG TCAGATCATC GAACAGGATC TACATGCTCT GCTGGATGTC
    ACTCCAAAGG CTGTAGACAC CCTCAATTAC ACTCAGTGGT ATCCCATTGT CATCTTCCTC
    AACCCAGACA GCAAGCAGGG GGTGAAGACC ATGAGGAATC GCTTGGTGCC TGGATCCAAC
    CGCAGCTCAC GCAAACTTTA CGAACAAGCG GTCAAACTGC GGAAGACATA TGCGCATCTG
    TTCACAGCTA CCATTGATCT GAACTCTTCT CACGATGCCT GGTTTGGAAG CCTCAAAGAC
    GCCGTACGAG AACAGCAAGA TAAAGCTGTC TGGGTCTGTC AAAGCAAGCT GGATGGCTCA
    GAGGAGGACC TGGATCTCCA TGATGACCGT ATGTCCTACC TGTCGGCCAT GAGTGCAGAT
    TACCTGAGCA TGGACAGCCG TCTGACCAGC GACTATGAGG ACACGGCGGA CGAGGGCGGC
    GCATACACTG ATAATGAGCT TGATGAGTCC ACAGATGAGC CTCAGCCAAT GTCTGCCATC
    AGCCGCTCAT CTGAACCCGT CAGTGAGGAG AGGCACATGC CTGTTCCCCA CGAGCGCTCA
    AAGAAACCAT CGGGCAGAGA CGTCCAGCGT GACCCCAGTC CTCCTCCGTC ATTTGTCCCA
    GAACCCCCAA AGGTGCGCTC TGCTTCCCGT CCTGCAGACT CCAGGAGTTT TGACTCGCGC
    TCCAGCAGCA CCATCAGCAG TGATGCGCCC ATCAGCAACA AACCTCTTCC TCCGCCCGTC
    GCCCAGAAGC CCAGTTTCAA TACGAGGTCG GCGGACGACA CACCTTCTAA TGACCCAGCA
    GATGACCCTG CCAACCGCAC CTTCAGGGGA AAGGTGAAGG CCTTCGAGCA GATGGACCAC
    CTTGCCAGAG CCAAACGTAT GCTGGAGCTG CAGGAGGCCG AACAAGCCCG GCTGGAAATC
    TCTCAGAAAC ATCCAGATAT TTATGCAGTT CCTAACAAGC CAAAACCAAA CCAGAACAGA
    CCACAACCAA TTGGCTCCAG CTCAAACTCC GAGTCCCAGA GCTCCTCCAA AGCAATGTAT
    TCAGAGAGAG GCTCACACTA CCGGCACGAT GATGATGACG AAGAGGAGTA TCGCCGCCAG
    CTGGCGGACC AGACCCGGAG AGGATATTAC AGCACACAGA AATACAATGA CACAGAGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303467.1
    CDS226..4068
    Translation

    Target ORF information:

    RefSeq Version XM_009305192.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 2a (zona occludens 2) (tjp2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305192.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGTACA AGAAATTCAT CACCATCATG CAAGCGGCCA TCGGGGTCGC ACCGCTCAAC 
    AAACGAGAGC TGATGCCACC CGGCCGGAGG TTGTGGAGAC CCTCAGGTGA CCAGACCAAC
    CAAATTGAGT TGTTCCGCTC AAGCCGTTCC AACAAGAAAA GGTTTTCCTT GACCTACGAA
    GCTCAATATC GGTGTCTGCG ACGCCTCTCT ACTCCAAGCT TCCCGGCCCT TTTACAGAAC
    CCAGTCATGG AGGAGACTGT GTGGGAGCAG TACACTGTGA CCCTACAGCG GGACTCCAAG
    ATGGGCTTTG GCCTAGCGGT GTCTGGAGGA CGGGACAATC CAAACGAAGA AAGTGGAGAA
    ATGTCAATCG TCATATCTGA TGTGCTGCAA GGGGGTCCAG CCGATGGCTT GCTGTTTGAA
    AATGACAGAG TGGTCCAGGT CAACACTGTG CCGATGGACG GTGTCCCACA CTCCTTTGCA
    GTCCAGACTC TCCGCAAATG TGGCAAAGTT GCCAAAATTA CTGTAAAGCG ATGTAGAAAA
    ATCCCTGTCA ACCTGACCAA GCGTGCTGCA TCCCCTGATG ACCGTGTCTT CAGTGGCGAC
    TACAACGATG ATTATGACTA TGAACAGGAC CAGCACAGCA TCTACAGTGG ACGCAGCTAC
    CCTAACCGTG ATACTAGTTT AGAGCGTGAG CGCGGCGGCT ATGCTGACGA GCGTGAACGG
    GACTATGATC GCAGTCGGGG GAGGAGCATG GAGCGCGGCG TGAGCCCTGA TAGACAATAC
    AGGCGGGACG GCAGCCGCGG ACGAATGCTG GACAACGAGC GCTGCCCCGA CCCCCGTTAC
    AGAAGTGAAA ATACCTTGGA TCGCGACCGC AGTCCAGACC CCCGTTATCG AAGTGAACGC
    ACGCTGGACC GCAACTACAG CCCAGAGCGA GGTCGCCGCT TTCGCAGCGA ACACAACCTG
    GACCGAGAGC GAAGTCCAGA CACACAATAC CGCAGCGATC GCACACTGGA CAGAACACAC
    AGTCCAGATA CACACTATAG GCCTGAACCT GCTCGGGGGT ACAGCAGAGA GAATCTCACC
    AGTGACAATG AGCGCAGGAG GTATGGCTCA CATCAGAACG AACCCATGAA GAAGAGCAAC
    AGCCAAGACA GATTGGATCG TTCGCCTTCA CCTGCACCAC TCCGACACAA TGCCTTGGAA
    AAGCCACTCA GTGTTACTCT CCTCAAAAAC CGTCCCAATG ATGAATATGG CCTTCGTCTA
    GGCAGTCAGT TGTTCATCAA AGAGATGACG AACACAGGCC TTGCATCCAG GGAAGGCAAA
    CTACAAGAAG GAGACATTAT TCTGAAAATC AACGGTACGG TCACAGAGAA TCTGTCTCTG
    AGCGATGCAG GGAAGCTGAT TGAGAAGTCT CGTGGAAAGC TGCAGCTGGT GGTGCAGAGG
    GATCGCAGTC AAGTGCTCAT CCGCATCCCA CCAATGGCAG ACAGCGACTC GGAAATGGAT
    GACATCTCTG AGATCGAGTC GTACCGCTCA TATTCTCCTC AGGAGGATCG GCGGAGTCAT
    CACTCAGACC TCTCCTCACA CTCCTCCAAT GAGAGATTAC AGGACAAAAA CAGAGAAGAG
    CCTCCTAAAA GACTGGCTAA AATGGGAGCG ATGCCCACTC CATTTGTATC AGCACCGGTG
    GATCTGCCAC CTCCACCTGT AGAGAAAGAA GAGCCTCGCA GCGAGTCTCC AGCTCCTCCT
    GTGGTAATTG CTGCTCCAAA AACTGTTCCA GTAAAAGCAA AACCTTTGCC AAAGGTTCCC
    TTGCTACCCA GTCCAGAAGA GCAGGAAATT TATGGGCCCA ACACTGTGAT GGTGCGCTTT
    GTGAAGGGTG AAAGCGTTGG TCTGCGTCTG GCTGGAGGAA ATGATGTGGG CATTTTCATC
    GCCGGCGTTC AAGAGGACAG TCCTGCCGAG GTGGAGGGTC TGCGCACTGG AGACCAAATA
    GTCAAGGTGA ATAACATGGA TTTTCGAGGC ATGGTCCGTG AAGATGCAGT TCTGTATTTA
    CTGGAGATTC CCAAAGGAGA GGATGTGACC ATTTTGGCTC AAAGCAAACC CGATGTGTAT
    AAAGACATCC TGGGGTCTGG ACGAGGTGAT AACTTCTTCA TCAGGACTCA TTTTGAATAT
    GAGAAAGAGC TTCCCCAGAG CCTGAACTTC TCTAGAGGAG AGGTTTTTAA AGTGGTGGAC
    ACCCTGTATG ATGGCAAGCT GGGAAACTGG CTCGCCATCC GCATGGACAA AGACAATCAG
    CTCCTGGAAA AGGGCATCAT ACCCAGCAAG AGCAGGGCGG AGCAGATGGC AAATGTTCAG
    CATGCACAGA AAGGTGCTGG TAATGACAGA GGCGATTTCT GGAGGCTCAG AGGTCAAAGG
    GCAGCTAAGA AAAAAGACCT TCGCAAGAGC AGAGAGGATC TCAGTGCAGC TCCAGTCACC
    ACACGCTTCC CGGCCTATGA GAGAGTGGTG CTGCGTGAAG CTGGCTTCAG GCGTCCAGTC
    GTGATTTTTG GCCCCATTTC AGATGCAGCC AACGAAAAAC TTGCCAATGA ACTTCCAAAT
    GAGTTCACCA TTGCAAAGAC CGAACCAAAA GACGCAGGCT CAGAAAAGTC ATCTGGCGTT
    GTGAGGCTAA ACACCATTCG TCAGATCATC GAACAGGATC TACATGCTCT GCTGGATGTC
    ACTCCAAAGG CTGTAGACAC CCTCAATTAC ACTCAGTGGT ATCCCATTGT CATCTTCCTC
    AACCCAGACA GCAAGCAGGG GGTGAAGACC ATGAGGAATC GCTTGGTGCC TGGATCCAAC
    CGCAGCTCAC GCAAACTTTA CGAACAAGCG GTCAAACTGC GGAAGACATA TGCGCATCTG
    TTCACAGCTA CCATTGATCT GAACTCTTCT CACGATGCCT GGTTTGGAAG CCTCAAAGAC
    GCCGTACGAG AACAGCAAGA TAAAGCTGTC TGGGTCTGTC AAAGCAAGCT GGATGGCTCA
    GAGGAGGACC TGGATCTCCA TGATGACCGT ATGTCCTACC TGTCGGCCAT GAGTGCAGAT
    TACCTGAGCA TGGACAGCCG TCTGACCAGC GACTATGAGG ACACGGCGGA CGAGGGCGGC
    GCATACACTG ATAATGAGCT TGATGAGTCC ACAGATGAGC CTCAGCCAAT GTCTGCCATC
    AGCCGCTCAT CTGAACCCGT CAGTGAGGAG AGGCACATGC CTGTTCCCCA CGAGCGCTCA
    AAGAAACCAT CGGGCAGAGA CGTCCAGCGT GACCCCAGTC CTCCTCCGTC ATTTGTCCCA
    GAACCCCCAA AGGTGCGCTC TGCTTCCCGT CCTGCAGACT CCAGGAGTTT TGACTCGCGC
    TCCAGCAGCA CCATCAGCAG TGATGCGCCC ATCAGCAACA AACCTCTTCC TCCGCCCGTC
    GCCCAGAAGC CCAGTTTCAA TACGAGGTCG GCGGACGACA CACCTTCTAA TGACCCAGCA
    GATGACCCTG CCAACCGCAC CTTCAGGGGA AAGGTGAAGG CCTTCGAGCA GATGGACCAC
    CTTGCCAGAG CCAAACGTAT GCTGGAGCTG CAGGAGGCCG AACAAGCCCG GCTGGAAATC
    TCTCAGAAAC ATCCAGATAT TTATGCAGTT CCTAACAAGC CAAAACCAAA CCAGAACAGA
    CCACAACCAA TTGGCTCCAG CTCAAACTCC GAGTCCCAGA GCTCCTCCAA AGCAATGTAT
    TCAGAGAGAG GCTCACACTA CCGGCACGAT GATGATGACG AAGAGGAGTA TCGCCGCCAG
    CTGGCGGACC AGACCCGGAG AGGATATTAC AGCACACAGA AATACAATGA CACAGAGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.