nf1b cDNA ORF clone, Danio rerio(zebrafish)

The following nf1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nf1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa54123 XM_017358082.1
Latest version!
Danio rerio neurofibromin 1b (nf1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa16410 XM_005169189.4
Latest version!
Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa16412 XM_005169190.4
Latest version!
Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa16410 XM_005169189.3 Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa16412 XM_005169190.3 Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa54123
    Clone ID Related Accession (Same CDS sequence) XM_017358082.1
    Accession Version XM_017358082.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA
    AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA
    GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG
    ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT
    CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT
    GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT
    TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG
    GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC
    AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC
    CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC
    AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA
    GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG
    TTTTACTATG TGACGCGCAG AGTTGGTCCA GTGTTAACCA TCACAAAGGT CAAAGTCCTA
    AAGTCAGATG AGGCGATCCG TCCATCAGCT GTGCTTTATT TTCCCCTGCA TTTCTATTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213571.1
    CDS283..4965
    Translation

    Target ORF information:

    RefSeq Version XM_017358082.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1b (nf1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA
    AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA
    GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG
    ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT
    CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT
    GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT
    TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG
    GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC
    AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC
    CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC
    AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA
    GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG
    TTTTACTATG TGACGCGCAG AGTTGGTCCA GTGTTAACCA TCACAAAGGT CAAAGTCCTA
    AAGTCAGATG AGGCGATCCG TCCATCAGCT GTGCTTTATT TTCCCCTGCA TTTCTATTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16410
    Clone ID Related Accession (Same CDS sequence) XM_005169189.3 , XM_005169189.4
    Accession Version XM_005169189.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA
    AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA
    GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG
    ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT
    CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT
    GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT
    TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG
    GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC
    AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC
    CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC
    AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA
    GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG
    TTTTACTATG TGACGCGCAG GTTCAAGACG GGCCAGATAA ATGGGGACTT GCTTATATAC
    CATGTCCTTC TCACCCTCAA ACCATACTAT GCCAAACCCT ATGAGCTAGT GGTGGATTTA
    ACCCACGCTG GTCCAAGCAA CCGCTTCAAG ACCGACTTCC TCTCCAAGTG GTTTGTCGTC
    TTTCCGGGAT TTGCATATGA AAACGTTGCT GCCATTTACA TCTATAACTG TAACACATGG
    GTCCGGGAAT ATACCAAATA TCATGAGAGA CTCTTGACGG GACTCAAAGG CAGTAAGAAG
    TTACTGTTCA TTGATTCGCC TGCACGGTTG GCTGAACATG TGGAACCAGA GCAACAGAAG
    CTTCCGGCAG CTACACTTGC ACTGGAAGAA GACCTGAAAG TCTTTCACAA TGCGCTAAAA
    CTGGCCCACA GAGACACCAA AGTATCTATT AAGGTGGGCA GCACAGCAGT GCAGGTCACC
    TCAGCCGAAC GAACACGAGT TTTGGGTCAA TCGGTCTTCC TCAACGACAT CTACTATGCT
    TCAGAGATCG AGGAGATTTG CCTAGTGGAC GAAAATCAAT TCACACTGAC TATCGCTAAC
    CAAGGGACGC CGCTGACCTT CATGCACCAG GAGTGTGAGA GCATAGTTCA CTCCATTATC
    CACATCCGCA CGCGCTGGGA GCTTTCCCAA CCTGACTCCA TTCCTCAACA CACCAAAATC
    AGGCCCAAAG ATGTTCCTGG GACCCTTCTC AACATCGCTC TCCTAAACCT GGGCAGCTCG
    GACCCCAGTT TAAGGTCTGC AGCTTATAAC CTCCTGTGCG CCTTAACATG CACCTTTAAC
    CTGAAGATCG AAGGGCAGCT TCTGGAGACG TCTGGCCTGT GCATCCCTGC CAATAACACT
    CTATTCATCG TGTCCATTAG CCAGACTCTA GCGGCTAATG AGCCTCACCT GACCCTGGAG
    TTCCTGGAGG AGTGTATCTC TGGATTCAGC AAGTCCAGTA TAGAACTAAA GCACCTGTGT
    TTAGAGTACA TGACTCCTTG GCTGCTGAAC CTGGTACGCT TCTGCAAGCA TAGCGATGAT
    GCCAAAAGGC AACGAGTCAC CGCCATTCTG GACAAACTCA TTACCATGAC CATCAACGAA
    AAGCAGATGT ATCCATCCAT TCAGGCCAAG ATCTGGGGGA GCTTGGGCCA GATAACTGAT
    CTCCTTGATG TAGTTCTGGA CAGTTTCATT AAAACCAGTG CGACAGGAGG TCTCGGATCC
    ATCAAAGCTG AAGTGATGGC GGACACTGCA GTGGCTTTGG CTTCTGGGAA TGTCAAATTG
    GTGTCAAGCA AGGTGATCAT TCGCATGTGC AAAATCATCG ATAAGACGTG TCTGTCGCCC
    ACACCAACAC TGGAGCAGCA CCTGATGTGG GATGATATCG CCATCCTGGC CCGGTACATG
    CTCATGCTGT CCTTCAACAA CTCTCTGGAT GTAGCCGCAC ACCTGCCGTA CCTGTTTCAT
    GTGGTGACCC TGCTGGTGGC CACGGGGCCT CTGTCTCTGC GGGCCTCCAC GCATGGCTTG
    GTCATCAACA TCATCCACTC CCTCTGCACA TGCTCCCAGC TGAGCTTCAG CGAGGAGACC
    AAGCAGGTGT TGCGTCTCAG TCTGACCGAG TTCTCCCTGC CGAAGTTCTA CCTGCTGTTC
    GGCATCAGCA AGGTCAAGTC GGCTGCGGTC ATTGCATTTC GTTCCAGCTA CAGGGATCGC
    TCATTCTCGC CGGGGTCCTA TGAGAGAGAG ACCTTTGCTC TGTCATCTCT GGAGACGGTC
    ACAGAGGCGC TGCTGGAGAT CATGGAGGCT TGCATGAGGG ACATTCCAGG CTGTAAATGG
    CTAGACCAGT GGACCGATCT GGCGCAGAAG TTTGCATTTC AATATAATCC ATCCCTCCAA
    CCACGGGCAC TTGTGGTATT CGGCTGCATC AGCAAACGAG TCACACATAC GCAGATGAAA
    CAGATCATAC GGATCTTGAG CAAGGCCAGC CTTCTCCTCA GCATCGACCG GGGTTTGGAG
    AGCTGCCTGA AGGGTCCCGA TAACTGTAAC AGCCAGGTGC TAATCGAAGC CACAGTCATT
    GCTCTCACTA AACTACAACC TTTGCTCAAC AAGGACTCAG CGATGCACAA AGCTCTGTTC
    TGGGTCGCTG TGGCCGTACT GCAGTTGGAT GAGGTGAATC TGTATTCTGC AGGAACTGCT
    CTGCTTGAAC AGAACCTACA CACACTCGAT AGCCTGAGAG TCTTCAACGA CAAGAGTCCT
    GAGGAGGTGT TTATGGAGAT CCGTCAGCCT CTGGAATGGC ACTGTAAACA AATGGACCAC
    TTTGTTGGCC TCAATTTTAA CTCCAACTTC AATTTTGCTT TGGTCGGTCA CCTGTTGAAA
    GGGTTCAGAC ACCCCTCCCC CACCACAGTG GCACGTACCA TCCGCATCCT GCACATGCTG
    TTGGCGTTAG TAGGAAAACA CCAGAACTGT GATAAATTTG AGGTGAACAC ACAGAGTGTG
    GCGTATCTGG CAGCGCTTCT CCCGGTGTCA GAGGAAGTGC GCAGCCGCTG CAGTCTGAAA
    CACAGAAAAT TGTTGCTTTT CTCTGATGTC ACAATGGAGA ATGTTCAAAT GGATCCGTAT
    TCTGTACATC ACGAGGATCC AAGCGGCAGA ACGTTGCGGG AAAACGAGCT GTGGGCATCC
    CCGAAAGTCT CAGAGCGATA CTTGGTGGCC CATTTCCCCA TAATGGGGCA GATCAGCCCA
    CGAACACGCA AATCAATGAG CTTGGACATG GGACAACCCT CTCAAGTCAA CACCAAGAAG
    CTTCTGGGCA CCAGAAAAAG CTTTGACCTC CTGTTATCAG ACAGCAAAGC ACCAAAGAGG
    CCTGAAATAG AGACTGGTAT CACAACGCCA CCCAAGATGA GGAGAGTGGC TGAAAACGAA
    TATGAAATCG AAACCCCAAG AATCTCCCAT CACCACTTGC GGAAGGTTTC TGTGTCCGAG
    TCCAATGTCC TGCTTAATGA AGAAGTTCTC ACAGATCCCA AAATCCAAGC ACTGCTTCTG
    ACTGTGCTGG CCACTCAGGT GAAAAACACT TCAGATGAAT TTGAGCAGAG GATCTTGTAT
    GAGTTTCTAG CCGAGGCCAG TGTTGTCTTC CCAAAAGTCT TTCCTGTTGT TCATAATCTT
    CTGGACTCTA AGATTAATAC GTTGTTGTCG CTGTGTCAGG ACCCACATCT TCTGAATCCA
    GTTCATGGAA TTGTTCAGAG TGTAGTTTAT CATGAAGAGT CTCCACCTCA GTACCAACCG
    TCCTACCTGC AAACTTTTGG GTTTAATGGA CTCTGGAGGT TTGCCGGTCC TTTTTCGAAG
    CAAACTCAGT TCCCAGACTA TGCAGAGCTG ATAGTCAAGT TTCTGGATGC ACTGATTGAC
    ACCTATCTTC CGGGCATTGA TGAGGAAACA AGCGAGGAGG GTCTGCGGAC ACCCACCTCT
    CCATACCCTC CACCTGTGCA GAGCCAGCTC AGCATCACTG CCAACCTCAA CCTCTCAAAT
    TCAATGACCT CGCTGGCCAC CTCCCAACAT GCACCAGGAA TGGATAAAGA GAAAGTGGAT
    CTATCCCCCA CCACGGGTTT GGCAGCCGGC GGACGCACAC GCCACGGCTC GGCCAGCCAG
    GTCCAGAAAC AGCGCAGCAC CGGCAGCTTT AAGAGACCTA GCATTAAAAA GATTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169246.1
    CDS279..8558
    Translation

    Target ORF information:

    RefSeq Version XM_005169189.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169189.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA
    AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA
    GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG
    ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT
    CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT
    GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT
    TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG
    GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC
    AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC
    CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC
    AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA
    GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG
    TTTTACTATG TGACGCGCAG GTTCAAGACG GGCCAGATAA ATGGGGACTT GCTTATATAC
    CATGTCCTTC TCACCCTCAA ACCATACTAT GCCAAACCCT ATGAGCTAGT GGTGGATTTA
    ACCCACGCTG GTCCAAGCAA CCGCTTCAAG ACCGACTTCC TCTCCAAGTG GTTTGTCGTC
    TTTCCGGGAT TTGCATATGA AAACGTTGCT GCCATTTACA TCTATAACTG TAACACATGG
    GTCCGGGAAT ATACCAAATA TCATGAGAGA CTCTTGACGG GACTCAAAGG CAGTAAGAAG
    TTACTGTTCA TTGATTCGCC TGCACGGTTG GCTGAACATG TGGAACCAGA GCAACAGAAG
    CTTCCGGCAG CTACACTTGC ACTGGAAGAA GACCTGAAAG TCTTTCACAA TGCGCTAAAA
    CTGGCCCACA GAGACACCAA AGTATCTATT AAGGTGGGCA GCACAGCAGT GCAGGTCACC
    TCAGCCGAAC GAACACGAGT TTTGGGTCAA TCGGTCTTCC TCAACGACAT CTACTATGCT
    TCAGAGATCG AGGAGATTTG CCTAGTGGAC GAAAATCAAT TCACACTGAC TATCGCTAAC
    CAAGGGACGC CGCTGACCTT CATGCACCAG GAGTGTGAGA GCATAGTTCA CTCCATTATC
    CACATCCGCA CGCGCTGGGA GCTTTCCCAA CCTGACTCCA TTCCTCAACA CACCAAAATC
    AGGCCCAAAG ATGTTCCTGG GACCCTTCTC AACATCGCTC TCCTAAACCT GGGCAGCTCG
    GACCCCAGTT TAAGGTCTGC AGCTTATAAC CTCCTGTGCG CCTTAACATG CACCTTTAAC
    CTGAAGATCG AAGGGCAGCT TCTGGAGACG TCTGGCCTGT GCATCCCTGC CAATAACACT
    CTATTCATCG TGTCCATTAG CCAGACTCTA GCGGCTAATG AGCCTCACCT GACCCTGGAG
    TTCCTGGAGG AGTGTATCTC TGGATTCAGC AAGTCCAGTA TAGAACTAAA GCACCTGTGT
    TTAGAGTACA TGACTCCTTG GCTGCTGAAC CTGGTACGCT TCTGCAAGCA TAGCGATGAT
    GCCAAAAGGC AACGAGTCAC CGCCATTCTG GACAAACTCA TTACCATGAC CATCAACGAA
    AAGCAGATGT ATCCATCCAT TCAGGCCAAG ATCTGGGGGA GCTTGGGCCA GATAACTGAT
    CTCCTTGATG TAGTTCTGGA CAGTTTCATT AAAACCAGTG CGACAGGAGG TCTCGGATCC
    ATCAAAGCTG AAGTGATGGC GGACACTGCA GTGGCTTTGG CTTCTGGGAA TGTCAAATTG
    GTGTCAAGCA AGGTGATCAT TCGCATGTGC AAAATCATCG ATAAGACGTG TCTGTCGCCC
    ACACCAACAC TGGAGCAGCA CCTGATGTGG GATGATATCG CCATCCTGGC CCGGTACATG
    CTCATGCTGT CCTTCAACAA CTCTCTGGAT GTAGCCGCAC ACCTGCCGTA CCTGTTTCAT
    GTGGTGACCC TGCTGGTGGC CACGGGGCCT CTGTCTCTGC GGGCCTCCAC GCATGGCTTG
    GTCATCAACA TCATCCACTC CCTCTGCACA TGCTCCCAGC TGAGCTTCAG CGAGGAGACC
    AAGCAGGTGT TGCGTCTCAG TCTGACCGAG TTCTCCCTGC CGAAGTTCTA CCTGCTGTTC
    GGCATCAGCA AGGTCAAGTC GGCTGCGGTC ATTGCATTTC GTTCCAGCTA CAGGGATCGC
    TCATTCTCGC CGGGGTCCTA TGAGAGAGAG ACCTTTGCTC TGTCATCTCT GGAGACGGTC
    ACAGAGGCGC TGCTGGAGAT CATGGAGGCT TGCATGAGGG ACATTCCAGG CTGTAAATGG
    CTAGACCAGT GGACCGATCT GGCGCAGAAG TTTGCATTTC AATATAATCC ATCCCTCCAA
    CCACGGGCAC TTGTGGTATT CGGCTGCATC AGCAAACGAG TCACACATAC GCAGATGAAA
    CAGATCATAC GGATCTTGAG CAAGGCCAGC CTTCTCCTCA GCATCGACCG GGGTTTGGAG
    AGCTGCCTGA AGGGTCCCGA TAACTGTAAC AGCCAGGTGC TAATCGAAGC CACAGTCATT
    GCTCTCACTA AACTACAACC TTTGCTCAAC AAGGACTCAG CGATGCACAA AGCTCTGTTC
    TGGGTCGCTG TGGCCGTACT GCAGTTGGAT GAGGTGAATC TGTATTCTGC AGGAACTGCT
    CTGCTTGAAC AGAACCTACA CACACTCGAT AGCCTGAGAG TCTTCAACGA CAAGAGTCCT
    GAGGAGGTGT TTATGGAGAT CCGTCAGCCT CTGGAATGGC ACTGTAAACA AATGGACCAC
    TTTGTTGGCC TCAATTTTAA CTCCAACTTC AATTTTGCTT TGGTCGGTCA CCTGTTGAAA
    GGGTTCAGAC ACCCCTCCCC CACCACAGTG GCACGTACCA TCCGCATCCT GCACATGCTG
    TTGGCGTTAG TAGGAAAACA CCAGAACTGT GATAAATTTG AGGTGAACAC ACAGAGTGTG
    GCGTATCTGG CAGCGCTTCT CCCGGTGTCA GAGGAAGTGC GCAGCCGCTG CAGTCTGAAA
    CACAGAAAAT TGTTGCTTTT CTCTGATGTC ACAATGGAGA ATGTTCAAAT GGATCCGTAT
    TCTGTACATC ACGAGGATCC AAGCGGCAGA ACGTTGCGGG AAAACGAGCT GTGGGCATCC
    CCGAAAGTCT CAGAGCGATA CTTGGTGGCC CATTTCCCCA TAATGGGGCA GATCAGCCCA
    CGAACACGCA AATCAATGAG CTTGGACATG GGACAACCCT CTCAAGTCAA CACCAAGAAG
    CTTCTGGGCA CCAGAAAAAG CTTTGACCTC CTGTTATCAG ACAGCAAAGC ACCAAAGAGG
    CCTGAAATAG AGACTGGTAT CACAACGCCA CCCAAGATGA GGAGAGTGGC TGAAAACGAA
    TATGAAATCG AAACCCCAAG AATCTCCCAT CACCACTTGC GGAAGGTTTC TGTGTCCGAG
    TCCAATGTCC TGCTTAATGA AGAAGTTCTC ACAGATCCCA AAATCCAAGC ACTGCTTCTG
    ACTGTGCTGG CCACTCAGGT GAAAAACACT TCAGATGAAT TTGAGCAGAG GATCTTGTAT
    GAGTTTCTAG CCGAGGCCAG TGTTGTCTTC CCAAAAGTCT TTCCTGTTGT TCATAATCTT
    CTGGACTCTA AGATTAATAC GTTGTTGTCG CTGTGTCAGG ACCCACATCT TCTGAATCCA
    GTTCATGGAA TTGTTCAGAG TGTAGTTTAT CATGAAGAGT CTCCACCTCA GTACCAACCG
    TCCTACCTGC AAACTTTTGG GTTTAATGGA CTCTGGAGGT TTGCCGGTCC TTTTTCGAAG
    CAAACTCAGT TCCCAGACTA TGCAGAGCTG ATAGTCAAGT TTCTGGATGC ACTGATTGAC
    ACCTATCTTC CGGGCATTGA TGAGGAAACA AGCGAGGAGG GTCTGCGGAC ACCCACCTCT
    CCATACCCTC CACCTGTGCA GAGCCAGCTC AGCATCACTG CCAACCTCAA CCTCTCAAAT
    TCAATGACCT CGCTGGCCAC CTCCCAACAT GCACCAGGAA TGGATAAAGA GAAAGTGGAT
    CTATCCCCCA CCACGGGTTT GGCAGCCGGC GGACGCACAC GCCACGGCTC GGCCAGCCAG
    GTCCAGAAAC AGCGCAGCAC CGGCAGCTTT AAGAGACCTA GCATTAAAAA GATTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16410
    Clone ID Related Accession (Same CDS sequence) XM_005169189.3 , XM_005169189.4
    Accession Version XM_005169189.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169189.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA
    AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA
    GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG
    ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT
    CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT
    GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT
    TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG
    GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC
    AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC
    CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC
    AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA
    GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG
    TTTTACTATG TGACGCGCAG GTTCAAGACG GGCCAGATAA ATGGGGACTT GCTTATATAC
    CATGTCCTTC TCACCCTCAA ACCATACTAT GCCAAACCCT ATGAGCTAGT GGTGGATTTA
    ACCCACGCTG GTCCAAGCAA CCGCTTCAAG ACCGACTTCC TCTCCAAGTG GTTTGTCGTC
    TTTCCGGGAT TTGCATATGA AAACGTTGCT GCCATTTACA TCTATAACTG TAACACATGG
    GTCCGGGAAT ATACCAAATA TCATGAGAGA CTCTTGACGG GACTCAAAGG CAGTAAGAAG
    TTACTGTTCA TTGATTCGCC TGCACGGTTG GCTGAACATG TGGAACCAGA GCAACAGAAG
    CTTCCGGCAG CTACACTTGC ACTGGAAGAA GACCTGAAAG TCTTTCACAA TGCGCTAAAA
    CTGGCCCACA GAGACACCAA AGTATCTATT AAGGTGGGCA GCACAGCAGT GCAGGTCACC
    TCAGCCGAAC GAACACGAGT TTTGGGTCAA TCGGTCTTCC TCAACGACAT CTACTATGCT
    TCAGAGATCG AGGAGATTTG CCTAGTGGAC GAAAATCAAT TCACACTGAC TATCGCTAAC
    CAAGGGACGC CGCTGACCTT CATGCACCAG GAGTGTGAGA GCATAGTTCA CTCCATTATC
    CACATCCGCA CGCGCTGGGA GCTTTCCCAA CCTGACTCCA TTCCTCAACA CACCAAAATC
    AGGCCCAAAG ATGTTCCTGG GACCCTTCTC AACATCGCTC TCCTAAACCT GGGCAGCTCG
    GACCCCAGTT TAAGGTCTGC AGCTTATAAC CTCCTGTGCG CCTTAACATG CACCTTTAAC
    CTGAAGATCG AAGGGCAGCT TCTGGAGACG TCTGGCCTGT GCATCCCTGC CAATAACACT
    CTATTCATCG TGTCCATTAG CCAGACTCTA GCGGCTAATG AGCCTCACCT GACCCTGGAG
    TTCCTGGAGG AGTGTATCTC TGGATTCAGC AAGTCCAGTA TAGAACTAAA GCACCTGTGT
    TTAGAGTACA TGACTCCTTG GCTGCTGAAC CTGGTACGCT TCTGCAAGCA TAGCGATGAT
    GCCAAAAGGC AACGAGTCAC CGCCATTCTG GACAAACTCA TTACCATGAC CATCAACGAA
    AAGCAGATGT ATCCATCCAT TCAGGCCAAG ATCTGGGGGA GCTTGGGCCA GATAACTGAT
    CTCCTTGATG TAGTTCTGGA CAGTTTCATT AAAACCAGTG CGACAGGAGG TCTCGGATCC
    ATCAAAGCTG AAGTGATGGC GGACACTGCA GTGGCTTTGG CTTCTGGGAA TGTCAAATTG
    GTGTCAAGCA AGGTGATCAT TCGCATGTGC AAAATCATCG ATAAGACGTG TCTGTCGCCC
    ACACCAACAC TGGAGCAGCA CCTGATGTGG GATGATATCG CCATCCTGGC CCGGTACATG
    CTCATGCTGT CCTTCAACAA CTCTCTGGAT GTAGCCGCAC ACCTGCCGTA CCTGTTTCAT
    GTGGTGACCC TGCTGGTGGC CACGGGGCCT CTGTCTCTGC GGGCCTCCAC GCATGGCTTG
    GTCATCAACA TCATCCACTC CCTCTGCACA TGCTCCCAGC TGAGCTTCAG CGAGGAGACC
    AAGCAGGTGT TGCGTCTCAG TCTGACCGAG TTCTCCCTGC CGAAGTTCTA CCTGCTGTTC
    GGCATCAGCA AGGTCAAGTC GGCTGCGGTC ATTGCATTTC GTTCCAGCTA CAGGGATCGC
    TCATTCTCGC CGGGGTCCTA TGAGAGAGAG ACCTTTGCTC TGTCATCTCT GGAGACGGTC
    ACAGAGGCGC TGCTGGAGAT CATGGAGGCT TGCATGAGGG ACATTCCAGG CTGTAAATGG
    CTAGACCAGT GGACCGATCT GGCGCAGAAG TTTGCATTTC AATATAATCC ATCCCTCCAA
    CCACGGGCAC TTGTGGTATT CGGCTGCATC AGCAAACGAG TCACACATAC GCAGATGAAA
    CAGATCATAC GGATCTTGAG CAAGGCCAGC CTTCTCCTCA GCATCGACCG GGGTTTGGAG
    AGCTGCCTGA AGGGTCCCGA TAACTGTAAC AGCCAGGTGC TAATCGAAGC CACAGTCATT
    GCTCTCACTA AACTACAACC TTTGCTCAAC AAGGACTCAG CGATGCACAA AGCTCTGTTC
    TGGGTCGCTG TGGCCGTACT GCAGTTGGAT GAGGTGAATC TGTATTCTGC AGGAACTGCT
    CTGCTTGAAC AGAACCTACA CACACTCGAT AGCCTGAGAG TCTTCAACGA CAAGAGTCCT
    GAGGAGGTGT TTATGGAGAT CCGTCAGCCT CTGGAATGGC ACTGTAAACA AATGGACCAC
    TTTGTTGGCC TCAATTTTAA CTCCAACTTC AATTTTGCTT TGGTCGGTCA CCTGTTGAAA
    GGGTTCAGAC ACCCCTCCCC CACCACAGTG GCACGTACCA TCCGCATCCT GCACATGCTG
    TTGGCGTTAG TAGGAAAACA CCAGAACTGT GATAAATTTG AGGTGAACAC ACAGAGTGTG
    GCGTATCTGG CAGCGCTTCT CCCGGTGTCA GAGGAAGTGC GCAGCCGCTG CAGTCTGAAA
    CACAGAAAAT TGTTGCTTTT CTCTGATGTC ACAATGGAGA ATGTTCAAAT GGATCCGTAT
    TCTGTACATC ACGAGGATCC AAGCGGCAGA ACGTTGCGGG AAAACGAGCT GTGGGCATCC
    CCGAAAGTCT CAGAGCGATA CTTGGTGGCC CATTTCCCCA TAATGGGGCA GATCAGCCCA
    CGAACACGCA AATCAATGAG CTTGGACATG GGACAACCCT CTCAAGTCAA CACCAAGAAG
    CTTCTGGGCA CCAGAAAAAG CTTTGACCTC CTGTTATCAG ACAGCAAAGC ACCAAAGAGG
    CCTGAAATAG AGACTGGTAT CACAACGCCA CCCAAGATGA GGAGAGTGGC TGAAAACGAA
    TATGAAATCG AAACCCCAAG AATCTCCCAT CACCACTTGC GGAAGGTTTC TGTGTCCGAG
    TCCAATGTCC TGCTTAATGA AGAAGTTCTC ACAGATCCCA AAATCCAAGC ACTGCTTCTG
    ACTGTGCTGG CCACTCAGGT GAAAAACACT TCAGATGAAT TTGAGCAGAG GATCTTGTAT
    GAGTTTCTAG CCGAGGCCAG TGTTGTCTTC CCAAAAGTCT TTCCTGTTGT TCATAATCTT
    CTGGACTCTA AGATTAATAC GTTGTTGTCG CTGTGTCAGG ACCCACATCT TCTGAATCCA
    GTTCATGGAA TTGTTCAGAG TGTAGTTTAT CATGAAGAGT CTCCACCTCA GTACCAACCG
    TCCTACCTGC AAACTTTTGG GTTTAATGGA CTCTGGAGGT TTGCCGGTCC TTTTTCGAAG
    CAAACTCAGT TCCCAGACTA TGCAGAGCTG ATAGTCAAGT TTCTGGATGC ACTGATTGAC
    ACCTATCTTC CGGGCATTGA TGAGGAAACA AGCGAGGAGG GTCTGCGGAC ACCCACCTCT
    CCATACCCTC CACCTGTGCA GAGCCAGCTC AGCATCACTG CCAACCTCAA CCTCTCAAAT
    TCAATGACCT CGCTGGCCAC CTCCCAACAT GCACCAGGAA TGGATAAAGA GAAAGTGGAT
    CTATCCCCCA CCACGGGTTT GGCAGCCGGC GGACGCACAC GCCACGGCTC GGCCAGCCAG
    GTCCAGAAAC AGCGCAGCAC CGGCAGCTTT AAGAGACCTA GCATTAAAAA GATTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169246.1
    CDS265..8544
    Translation

    Target ORF information:

    RefSeq Version XM_005169189.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169189.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA
    AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA
    GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG
    ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT
    CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT
    GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT
    TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG
    GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC
    AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC
    CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC
    AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA
    GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG
    TTTTACTATG TGACGCGCAG GTTCAAGACG GGCCAGATAA ATGGGGACTT GCTTATATAC
    CATGTCCTTC TCACCCTCAA ACCATACTAT GCCAAACCCT ATGAGCTAGT GGTGGATTTA
    ACCCACGCTG GTCCAAGCAA CCGCTTCAAG ACCGACTTCC TCTCCAAGTG GTTTGTCGTC
    TTTCCGGGAT TTGCATATGA AAACGTTGCT GCCATTTACA TCTATAACTG TAACACATGG
    GTCCGGGAAT ATACCAAATA TCATGAGAGA CTCTTGACGG GACTCAAAGG CAGTAAGAAG
    TTACTGTTCA TTGATTCGCC TGCACGGTTG GCTGAACATG TGGAACCAGA GCAACAGAAG
    CTTCCGGCAG CTACACTTGC ACTGGAAGAA GACCTGAAAG TCTTTCACAA TGCGCTAAAA
    CTGGCCCACA GAGACACCAA AGTATCTATT AAGGTGGGCA GCACAGCAGT GCAGGTCACC
    TCAGCCGAAC GAACACGAGT TTTGGGTCAA TCGGTCTTCC TCAACGACAT CTACTATGCT
    TCAGAGATCG AGGAGATTTG CCTAGTGGAC GAAAATCAAT TCACACTGAC TATCGCTAAC
    CAAGGGACGC CGCTGACCTT CATGCACCAG GAGTGTGAGA GCATAGTTCA CTCCATTATC
    CACATCCGCA CGCGCTGGGA GCTTTCCCAA CCTGACTCCA TTCCTCAACA CACCAAAATC
    AGGCCCAAAG ATGTTCCTGG GACCCTTCTC AACATCGCTC TCCTAAACCT GGGCAGCTCG
    GACCCCAGTT TAAGGTCTGC AGCTTATAAC CTCCTGTGCG CCTTAACATG CACCTTTAAC
    CTGAAGATCG AAGGGCAGCT TCTGGAGACG TCTGGCCTGT GCATCCCTGC CAATAACACT
    CTATTCATCG TGTCCATTAG CCAGACTCTA GCGGCTAATG AGCCTCACCT GACCCTGGAG
    TTCCTGGAGG AGTGTATCTC TGGATTCAGC AAGTCCAGTA TAGAACTAAA GCACCTGTGT
    TTAGAGTACA TGACTCCTTG GCTGCTGAAC CTGGTACGCT TCTGCAAGCA TAGCGATGAT
    GCCAAAAGGC AACGAGTCAC CGCCATTCTG GACAAACTCA TTACCATGAC CATCAACGAA
    AAGCAGATGT ATCCATCCAT TCAGGCCAAG ATCTGGGGGA GCTTGGGCCA GATAACTGAT
    CTCCTTGATG TAGTTCTGGA CAGTTTCATT AAAACCAGTG CGACAGGAGG TCTCGGATCC
    ATCAAAGCTG AAGTGATGGC GGACACTGCA GTGGCTTTGG CTTCTGGGAA TGTCAAATTG
    GTGTCAAGCA AGGTGATCAT TCGCATGTGC AAAATCATCG ATAAGACGTG TCTGTCGCCC
    ACACCAACAC TGGAGCAGCA CCTGATGTGG GATGATATCG CCATCCTGGC CCGGTACATG
    CTCATGCTGT CCTTCAACAA CTCTCTGGAT GTAGCCGCAC ACCTGCCGTA CCTGTTTCAT
    GTGGTGACCC TGCTGGTGGC CACGGGGCCT CTGTCTCTGC GGGCCTCCAC GCATGGCTTG
    GTCATCAACA TCATCCACTC CCTCTGCACA TGCTCCCAGC TGAGCTTCAG CGAGGAGACC
    AAGCAGGTGT TGCGTCTCAG TCTGACCGAG TTCTCCCTGC CGAAGTTCTA CCTGCTGTTC
    GGCATCAGCA AGGTCAAGTC GGCTGCGGTC ATTGCATTTC GTTCCAGCTA CAGGGATCGC
    TCATTCTCGC CGGGGTCCTA TGAGAGAGAG ACCTTTGCTC TGTCATCTCT GGAGACGGTC
    ACAGAGGCGC TGCTGGAGAT CATGGAGGCT TGCATGAGGG ACATTCCAGG CTGTAAATGG
    CTAGACCAGT GGACCGATCT GGCGCAGAAG TTTGCATTTC AATATAATCC ATCCCTCCAA
    CCACGGGCAC TTGTGGTATT CGGCTGCATC AGCAAACGAG TCACACATAC GCAGATGAAA
    CAGATCATAC GGATCTTGAG CAAGGCCAGC CTTCTCCTCA GCATCGACCG GGGTTTGGAG
    AGCTGCCTGA AGGGTCCCGA TAACTGTAAC AGCCAGGTGC TAATCGAAGC CACAGTCATT
    GCTCTCACTA AACTACAACC TTTGCTCAAC AAGGACTCAG CGATGCACAA AGCTCTGTTC
    TGGGTCGCTG TGGCCGTACT GCAGTTGGAT GAGGTGAATC TGTATTCTGC AGGAACTGCT
    CTGCTTGAAC AGAACCTACA CACACTCGAT AGCCTGAGAG TCTTCAACGA CAAGAGTCCT
    GAGGAGGTGT TTATGGAGAT CCGTCAGCCT CTGGAATGGC ACTGTAAACA AATGGACCAC
    TTTGTTGGCC TCAATTTTAA CTCCAACTTC AATTTTGCTT TGGTCGGTCA CCTGTTGAAA
    GGGTTCAGAC ACCCCTCCCC CACCACAGTG GCACGTACCA TCCGCATCCT GCACATGCTG
    TTGGCGTTAG TAGGAAAACA CCAGAACTGT GATAAATTTG AGGTGAACAC ACAGAGTGTG
    GCGTATCTGG CAGCGCTTCT CCCGGTGTCA GAGGAAGTGC GCAGCCGCTG CAGTCTGAAA
    CACAGAAAAT TGTTGCTTTT CTCTGATGTC ACAATGGAGA ATGTTCAAAT GGATCCGTAT
    TCTGTACATC ACGAGGATCC AAGCGGCAGA ACGTTGCGGG AAAACGAGCT GTGGGCATCC
    CCGAAAGTCT CAGAGCGATA CTTGGTGGCC CATTTCCCCA TAATGGGGCA GATCAGCCCA
    CGAACACGCA AATCAATGAG CTTGGACATG GGACAACCCT CTCAAGTCAA CACCAAGAAG
    CTTCTGGGCA CCAGAAAAAG CTTTGACCTC CTGTTATCAG ACAGCAAAGC ACCAAAGAGG
    CCTGAAATAG AGACTGGTAT CACAACGCCA CCCAAGATGA GGAGAGTGGC TGAAAACGAA
    TATGAAATCG AAACCCCAAG AATCTCCCAT CACCACTTGC GGAAGGTTTC TGTGTCCGAG
    TCCAATGTCC TGCTTAATGA AGAAGTTCTC ACAGATCCCA AAATCCAAGC ACTGCTTCTG
    ACTGTGCTGG CCACTCAGGT GAAAAACACT TCAGATGAAT TTGAGCAGAG GATCTTGTAT
    GAGTTTCTAG CCGAGGCCAG TGTTGTCTTC CCAAAAGTCT TTCCTGTTGT TCATAATCTT
    CTGGACTCTA AGATTAATAC GTTGTTGTCG CTGTGTCAGG ACCCACATCT TCTGAATCCA
    GTTCATGGAA TTGTTCAGAG TGTAGTTTAT CATGAAGAGT CTCCACCTCA GTACCAACCG
    TCCTACCTGC AAACTTTTGG GTTTAATGGA CTCTGGAGGT TTGCCGGTCC TTTTTCGAAG
    CAAACTCAGT TCCCAGACTA TGCAGAGCTG ATAGTCAAGT TTCTGGATGC ACTGATTGAC
    ACCTATCTTC CGGGCATTGA TGAGGAAACA AGCGAGGAGG GTCTGCGGAC ACCCACCTCT
    CCATACCCTC CACCTGTGCA GAGCCAGCTC AGCATCACTG CCAACCTCAA CCTCTCAAAT
    TCAATGACCT CGCTGGCCAC CTCCCAACAT GCACCAGGAA TGGATAAAGA GAAAGTGGAT
    CTATCCCCCA CCACGGGTTT GGCAGCCGGC GGACGCACAC GCCACGGCTC GGCCAGCCAG
    GTCCAGAAAC AGCGCAGCAC CGGCAGCTTT AAGAGACCTA GCATTAAAAA GATTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16412
    Clone ID Related Accession (Same CDS sequence) XM_005169190.3 , XM_005169190.4
    Accession Version XM_005169190.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGTCGT GAGCCAGCGT TTTCCTCAGA ACAGCATTGG AGCTGTAGGC
    AGTGCCATGT TCTTGCGATT CGTGAATCCA GCTATAGTCT CTCCATACGA GGCTGGGATT
    CTTGACAAAA AACCTCAGCC TAGGATAGAA AGAGGCCTAA AACTCATGTC CAAGATTCTC
    CAAAGTATTG CCAACCATGT TTTGTTCACA AAAGAGGAGC ACATGAGGCC TTTCAATGAC
    TTTGTGAAGA GTAATTTTGA TGCTGCCAGA AGATTTTTCC TGGACATCGC CTCTGATTGC
    CCTGCTAGCG ACTCTCTCAA CCATAGCCTG TCCTTTATTA GTGATGGGAA CGTTCTGGCT
    CTGCACAGAC TGCTGTGGAA CAATCAAGAA AAAATTGGCC AGTATTTATC TAGCAACAGG
    GATCATAAAG CAGTCGGAAG AAGGCCTTTT GATAAGATGG CCACCCTGCT CGCTTACCTG
    GGTCCCCCAG AACACAAACC TGTGGCAGAT ACTCACTGGT CCAGTTTGAA TCTTACCAGC
    TCCAAATTTG AGGAGTTCAT GACAAGGCAC CAGGTCCATG AGAAAGAAGA GTTCAAAGCA
    TTGAAGACTC TACACATCTT CTACCAGGCT GGAACATCAA AGAACGGAAA CCCTGTGTTT
    TACTATGTGA CGCGCAGGTT CAAGACGGGC CAGATAAATG GGGACTTGCT TATATACCAT
    GTCCTTCTCA CCCTCAAACC ATACTATGCC AAACCCTATG AGCTAGTGGT GGATTTAACC
    CACGCTGGTC CAAGCAACCG CTTCAAGACC GACTTCCTCT CCAAGTGGTT TGTCGTCTTT
    CCGGGATTTG CATATGAAAA CGTTGCTGCC ATTTACATCT ATAACTGTAA CACATGGGTC
    CGGGAATATA CCAAATATCA TGAGAGACTC TTGACGGGAC TCAAAGGCAG TAAGAAGTTA
    CTGTTCATTG ATTCGCCTGC ACGGTTGGCT GAACATGTGG AACCAGAGCA ACAGAAGCTT
    CCGGCAGCTA CACTTGCACT GGAAGAAGAC CTGAAAGTCT TTCACAATGC GCTAAAACTG
    GCCCACAGAG ACACCAAAGT ATCTATTAAG GTGGGCAGCA CAGCAGTGCA GGTCACCTCA
    GCCGAACGAA CACGAGTTTT GGGTCAATCG GTCTTCCTCA ACGACATCTA CTATGCTTCA
    GAGATCGAGG AGATTTGCCT AGTGGACGAA AATCAATTCA CACTGACTAT CGCTAACCAA
    GGGACGCCGC TGACCTTCAT GCACCAGGAG TGTGAGAGCA TAGTTCACTC CATTATCCAC
    ATCCGCACGC GCTGGGAGCT TTCCCAACCT GACTCCATTC CTCAACACAC CAAAATCAGG
    CCCAAAGATG TTCCTGGGAC CCTTCTCAAC ATCGCTCTCC TAAACCTGGG CAGCTCGGAC
    CCCAGTTTAA GGTCTGCAGC TTATAACCTC CTGTGCGCCT TAACATGCAC CTTTAACCTG
    AAGATCGAAG GGCAGCTTCT GGAGACGTCT GGCCTGTGCA TCCCTGCCAA TAACACTCTA
    TTCATCGTGT CCATTAGCCA GACTCTAGCG GCTAATGAGC CTCACCTGAC CCTGGAGTTC
    CTGGAGGAGT GTATCTCTGG ATTCAGCAAG TCCAGTATAG AACTAAAGCA CCTGTGTTTA
    GAGTACATGA CTCCTTGGCT GCTGAACCTG GTACGCTTCT GCAAGCATAG CGATGATGCC
    AAAAGGCAAC GAGTCACCGC CATTCTGGAC AAACTCATTA CCATGACCAT CAACGAAAAG
    CAGATGTATC CATCCATTCA GGCCAAGATC TGGGGGAGCT TGGGCCAGAT AACTGATCTC
    CTTGATGTAG TTCTGGACAG TTTCATTAAA ACCAGTGCGA CAGGAGGTCT CGGATCCATC
    AAAGCTGAAG TGATGGCGGA CACTGCAGTG GCTTTGGCTT CTGGGAATGT CAAATTGGTG
    TCAAGCAAGG TGATCATTCG CATGTGCAAA ATCATCGATA AGACGTGTCT GTCGCCCACA
    CCAACACTGG AGCAGCACCT GATGTGGGAT GATATCGCCA TCCTGGCCCG GTACATGCTC
    ATGCTGTCCT TCAACAACTC TCTGGATGTA GCCGCACACC TGCCGTACCT GTTTCATGTG
    GTGACCCTGC TGGTGGCCAC GGGGCCTCTG TCTCTGCGGG CCTCCACGCA TGGCTTGGTC
    ATCAACATCA TCCACTCCCT CTGCACATGC TCCCAGCTGA GCTTCAGCGA GGAGACCAAG
    CAGGTGTTGC GTCTCAGTCT GACCGAGTTC TCCCTGCCGA AGTTCTACCT GCTGTTCGGC
    ATCAGCAAGG TCAAGTCGGC TGCGGTCATT GCATTTCGTT CCAGCTACAG GGATCGCTCA
    TTCTCGCCGG GGTCCTATGA GAGAGAGACC TTTGCTCTGT CATCTCTGGA GACGGTCACA
    GAGGCGCTGC TGGAGATCAT GGAGGCTTGC ATGAGGGACA TTCCAGGCTG TAAATGGCTA
    GACCAGTGGA CCGATCTGGC GCAGAAGTTT GCATTTCAAT ATAATCCATC CCTCCAACCA
    CGGGCACTTG TGGTATTCGG CTGCATCAGC AAACGAGTCA CACATACGCA GATGAAACAG
    ATCATACGGA TCTTGAGCAA GGCCAGCCTT CTCCTCAGCA TCGACCGGGG TTTGGAGAGC
    TGCCTGAAGG GTCCCGATAA CTGTAACAGC CAGGTGCTAA TCGAAGCCAC AGTCATTGCT
    CTCACTAAAC TACAACCTTT GCTCAACAAG GACTCAGCGA TGCACAAAGC TCTGTTCTGG
    GTCGCTGTGG CCGTACTGCA GTTGGATGAG GTGAATCTGT ATTCTGCAGG AACTGCTCTG
    CTTGAACAGA ACCTACACAC ACTCGATAGC CTGAGAGTCT TCAACGACAA GAGTCCTGAG
    GAGGTGTTTA TGGAGATCCG TCAGCCTCTG GAATGGCACT GTAAACAAAT GGACCACTTT
    GTTGGCCTCA ATTTTAACTC CAACTTCAAT TTTGCTTTGG TCGGTCACCT GTTGAAAGGG
    TTCAGACACC CCTCCCCCAC CACAGTGGCA CGTACCATCC GCATCCTGCA CATGCTGTTG
    GCGTTAGTAG GAAAACACCA GAACTGTGAT AAATTTGAGG TGAACACACA GAGTGTGGCG
    TATCTGGCAG CGCTTCTCCC GGTGTCAGAG GAAGTGCGCA GCCGCTGCAG TCTGAAACAC
    AGAAAATTGT TGCTTTTCTC TGATGTCACA ATGGAGAATG TTCAAATGGA TCCGTATTCT
    GTACATCACG AGGATCCAAG CGGCAGAACG TTGCGGGAAA ACGAGCTGTG GGCATCCCCG
    AAAGTCTCAG AGCGATACTT GGTGGCCCAT TTCCCCATAA TGGGGCAGAT CAGCCCACGA
    ACACGCAAAT CAATGAGCTT GGACATGGGA CAACCCTCTC AAGTCAACAC CAAGAAGCTT
    CTGGGCACCA GAAAAAGCTT TGACCTCCTG TTATCAGACA GCAAAGCACC AAAGAGGCCT
    GAAATAGAGA CTGGTATCAC AACGCCACCC AAGATGAGGA GAGTGGCTGA AAACGAATAT
    GAAATCGAAA CCCCAAGAAT CTCCCATCAC CACTTGCGGA AGGTTTCTGT GTCCGAGTCC
    AATGTCCTGC TTAATGAAGA AGTTCTCACA GATCCCAAAA TCCAAGCACT GCTTCTGACT
    GTGCTGGCCA CTCAGGTGAA AAACACTTCA GATGAATTTG AGCAGAGGAT CTTGTATGAG
    TTTCTAGCCG AGGCCAGTGT TGTCTTCCCA AAAGTCTTTC CTGTTGTTCA TAATCTTCTG
    GACTCTAAGA TTAATACGTT GTTGTCGCTG TGTCAGGACC CACATCTTCT GAATCCAGTT
    CATGGAATTG TTCAGAGTGT AGTTTATCAT GAAGAGTCTC CACCTCAGTA CCAACCGTCC
    TACCTGCAAA CTTTTGGGTT TAATGGACTC TGGAGGTTTG CCGGTCCTTT TTCGAAGCAA
    ACTCAGTTCC CAGACTATGC AGAGCTGATA GTCAAGTTTC TGGATGCACT GATTGACACC
    TATCTTCCGG GCATTGATGA GGAAACAAGC GAGGAGGGTC TGCGGACACC CACCTCTCCA
    TACCCTCCAC CTGTGCAGAG CCAGCTCAGC ATCACTGCCA ACCTCAACCT CTCAAATTCA
    ATGACCTCGC TGGCCACCTC CCAACATGCA CCAGGAATGG ATAAAGAGAA AGTGGATCTA
    TCCCCCACCA CGGGTTTGGC AGCCGGCGGA CGCACACGCC ACGGCTCGGC CAGCCAGGTC
    CAGAAACAGC GCAGCACCGG CAGCTTTAAG AGACCTAGCA TTAAAAAGAT TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169247.1
    CDS279..8495
    Translation

    Target ORF information:

    RefSeq Version XM_005169190.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169190.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGTCGT GAGCCAGCGT TTTCCTCAGA ACAGCATTGG AGCTGTAGGC
    AGTGCCATGT TCTTGCGATT CGTGAATCCA GCTATAGTCT CTCCATACGA GGCTGGGATT
    CTTGACAAAA AACCTCAGCC TAGGATAGAA AGAGGCCTAA AACTCATGTC CAAGATTCTC
    CAAAGTATTG CCAACCATGT TTTGTTCACA AAAGAGGAGC ACATGAGGCC TTTCAATGAC
    TTTGTGAAGA GTAATTTTGA TGCTGCCAGA AGATTTTTCC TGGACATCGC CTCTGATTGC
    CCTGCTAGCG ACTCTCTCAA CCATAGCCTG TCCTTTATTA GTGATGGGAA CGTTCTGGCT
    CTGCACAGAC TGCTGTGGAA CAATCAAGAA AAAATTGGCC AGTATTTATC TAGCAACAGG
    GATCATAAAG CAGTCGGAAG AAGGCCTTTT GATAAGATGG CCACCCTGCT CGCTTACCTG
    GGTCCCCCAG AACACAAACC TGTGGCAGAT ACTCACTGGT CCAGTTTGAA TCTTACCAGC
    TCCAAATTTG AGGAGTTCAT GACAAGGCAC CAGGTCCATG AGAAAGAAGA GTTCAAAGCA
    TTGAAGACTC TACACATCTT CTACCAGGCT GGAACATCAA AGAACGGAAA CCCTGTGTTT
    TACTATGTGA CGCGCAGGTT CAAGACGGGC CAGATAAATG GGGACTTGCT TATATACCAT
    GTCCTTCTCA CCCTCAAACC ATACTATGCC AAACCCTATG AGCTAGTGGT GGATTTAACC
    CACGCTGGTC CAAGCAACCG CTTCAAGACC GACTTCCTCT CCAAGTGGTT TGTCGTCTTT
    CCGGGATTTG CATATGAAAA CGTTGCTGCC ATTTACATCT ATAACTGTAA CACATGGGTC
    CGGGAATATA CCAAATATCA TGAGAGACTC TTGACGGGAC TCAAAGGCAG TAAGAAGTTA
    CTGTTCATTG ATTCGCCTGC ACGGTTGGCT GAACATGTGG AACCAGAGCA ACAGAAGCTT
    CCGGCAGCTA CACTTGCACT GGAAGAAGAC CTGAAAGTCT TTCACAATGC GCTAAAACTG
    GCCCACAGAG ACACCAAAGT ATCTATTAAG GTGGGCAGCA CAGCAGTGCA GGTCACCTCA
    GCCGAACGAA CACGAGTTTT GGGTCAATCG GTCTTCCTCA ACGACATCTA CTATGCTTCA
    GAGATCGAGG AGATTTGCCT AGTGGACGAA AATCAATTCA CACTGACTAT CGCTAACCAA
    GGGACGCCGC TGACCTTCAT GCACCAGGAG TGTGAGAGCA TAGTTCACTC CATTATCCAC
    ATCCGCACGC GCTGGGAGCT TTCCCAACCT GACTCCATTC CTCAACACAC CAAAATCAGG
    CCCAAAGATG TTCCTGGGAC CCTTCTCAAC ATCGCTCTCC TAAACCTGGG CAGCTCGGAC
    CCCAGTTTAA GGTCTGCAGC TTATAACCTC CTGTGCGCCT TAACATGCAC CTTTAACCTG
    AAGATCGAAG GGCAGCTTCT GGAGACGTCT GGCCTGTGCA TCCCTGCCAA TAACACTCTA
    TTCATCGTGT CCATTAGCCA GACTCTAGCG GCTAATGAGC CTCACCTGAC CCTGGAGTTC
    CTGGAGGAGT GTATCTCTGG ATTCAGCAAG TCCAGTATAG AACTAAAGCA CCTGTGTTTA
    GAGTACATGA CTCCTTGGCT GCTGAACCTG GTACGCTTCT GCAAGCATAG CGATGATGCC
    AAAAGGCAAC GAGTCACCGC CATTCTGGAC AAACTCATTA CCATGACCAT CAACGAAAAG
    CAGATGTATC CATCCATTCA GGCCAAGATC TGGGGGAGCT TGGGCCAGAT AACTGATCTC
    CTTGATGTAG TTCTGGACAG TTTCATTAAA ACCAGTGCGA CAGGAGGTCT CGGATCCATC
    AAAGCTGAAG TGATGGCGGA CACTGCAGTG GCTTTGGCTT CTGGGAATGT CAAATTGGTG
    TCAAGCAAGG TGATCATTCG CATGTGCAAA ATCATCGATA AGACGTGTCT GTCGCCCACA
    CCAACACTGG AGCAGCACCT GATGTGGGAT GATATCGCCA TCCTGGCCCG GTACATGCTC
    ATGCTGTCCT TCAACAACTC TCTGGATGTA GCCGCACACC TGCCGTACCT GTTTCATGTG
    GTGACCCTGC TGGTGGCCAC GGGGCCTCTG TCTCTGCGGG CCTCCACGCA TGGCTTGGTC
    ATCAACATCA TCCACTCCCT CTGCACATGC TCCCAGCTGA GCTTCAGCGA GGAGACCAAG
    CAGGTGTTGC GTCTCAGTCT GACCGAGTTC TCCCTGCCGA AGTTCTACCT GCTGTTCGGC
    ATCAGCAAGG TCAAGTCGGC TGCGGTCATT GCATTTCGTT CCAGCTACAG GGATCGCTCA
    TTCTCGCCGG GGTCCTATGA GAGAGAGACC TTTGCTCTGT CATCTCTGGA GACGGTCACA
    GAGGCGCTGC TGGAGATCAT GGAGGCTTGC ATGAGGGACA TTCCAGGCTG TAAATGGCTA
    GACCAGTGGA CCGATCTGGC GCAGAAGTTT GCATTTCAAT ATAATCCATC CCTCCAACCA
    CGGGCACTTG TGGTATTCGG CTGCATCAGC AAACGAGTCA CACATACGCA GATGAAACAG
    ATCATACGGA TCTTGAGCAA GGCCAGCCTT CTCCTCAGCA TCGACCGGGG TTTGGAGAGC
    TGCCTGAAGG GTCCCGATAA CTGTAACAGC CAGGTGCTAA TCGAAGCCAC AGTCATTGCT
    CTCACTAAAC TACAACCTTT GCTCAACAAG GACTCAGCGA TGCACAAAGC TCTGTTCTGG
    GTCGCTGTGG CCGTACTGCA GTTGGATGAG GTGAATCTGT ATTCTGCAGG AACTGCTCTG
    CTTGAACAGA ACCTACACAC ACTCGATAGC CTGAGAGTCT TCAACGACAA GAGTCCTGAG
    GAGGTGTTTA TGGAGATCCG TCAGCCTCTG GAATGGCACT GTAAACAAAT GGACCACTTT
    GTTGGCCTCA ATTTTAACTC CAACTTCAAT TTTGCTTTGG TCGGTCACCT GTTGAAAGGG
    TTCAGACACC CCTCCCCCAC CACAGTGGCA CGTACCATCC GCATCCTGCA CATGCTGTTG
    GCGTTAGTAG GAAAACACCA GAACTGTGAT AAATTTGAGG TGAACACACA GAGTGTGGCG
    TATCTGGCAG CGCTTCTCCC GGTGTCAGAG GAAGTGCGCA GCCGCTGCAG TCTGAAACAC
    AGAAAATTGT TGCTTTTCTC TGATGTCACA ATGGAGAATG TTCAAATGGA TCCGTATTCT
    GTACATCACG AGGATCCAAG CGGCAGAACG TTGCGGGAAA ACGAGCTGTG GGCATCCCCG
    AAAGTCTCAG AGCGATACTT GGTGGCCCAT TTCCCCATAA TGGGGCAGAT CAGCCCACGA
    ACACGCAAAT CAATGAGCTT GGACATGGGA CAACCCTCTC AAGTCAACAC CAAGAAGCTT
    CTGGGCACCA GAAAAAGCTT TGACCTCCTG TTATCAGACA GCAAAGCACC AAAGAGGCCT
    GAAATAGAGA CTGGTATCAC AACGCCACCC AAGATGAGGA GAGTGGCTGA AAACGAATAT
    GAAATCGAAA CCCCAAGAAT CTCCCATCAC CACTTGCGGA AGGTTTCTGT GTCCGAGTCC
    AATGTCCTGC TTAATGAAGA AGTTCTCACA GATCCCAAAA TCCAAGCACT GCTTCTGACT
    GTGCTGGCCA CTCAGGTGAA AAACACTTCA GATGAATTTG AGCAGAGGAT CTTGTATGAG
    TTTCTAGCCG AGGCCAGTGT TGTCTTCCCA AAAGTCTTTC CTGTTGTTCA TAATCTTCTG
    GACTCTAAGA TTAATACGTT GTTGTCGCTG TGTCAGGACC CACATCTTCT GAATCCAGTT
    CATGGAATTG TTCAGAGTGT AGTTTATCAT GAAGAGTCTC CACCTCAGTA CCAACCGTCC
    TACCTGCAAA CTTTTGGGTT TAATGGACTC TGGAGGTTTG CCGGTCCTTT TTCGAAGCAA
    ACTCAGTTCC CAGACTATGC AGAGCTGATA GTCAAGTTTC TGGATGCACT GATTGACACC
    TATCTTCCGG GCATTGATGA GGAAACAAGC GAGGAGGGTC TGCGGACACC CACCTCTCCA
    TACCCTCCAC CTGTGCAGAG CCAGCTCAGC ATCACTGCCA ACCTCAACCT CTCAAATTCA
    ATGACCTCGC TGGCCACCTC CCAACATGCA CCAGGAATGG ATAAAGAGAA AGTGGATCTA
    TCCCCCACCA CGGGTTTGGC AGCCGGCGGA CGCACACGCC ACGGCTCGGC CAGCCAGGTC
    CAGAAACAGC GCAGCACCGG CAGCTTTAAG AGACCTAGCA TTAAAAAGAT TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16412
    Clone ID Related Accession (Same CDS sequence) XM_005169190.3 , XM_005169190.4
    Accession Version XM_005169190.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169190.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGTCGT GAGCCAGCGT TTTCCTCAGA ACAGCATTGG AGCTGTAGGC
    AGTGCCATGT TCTTGCGATT CGTGAATCCA GCTATAGTCT CTCCATACGA GGCTGGGATT
    CTTGACAAAA AACCTCAGCC TAGGATAGAA AGAGGCCTAA AACTCATGTC CAAGATTCTC
    CAAAGTATTG CCAACCATGT TTTGTTCACA AAAGAGGAGC ACATGAGGCC TTTCAATGAC
    TTTGTGAAGA GTAATTTTGA TGCTGCCAGA AGATTTTTCC TGGACATCGC CTCTGATTGC
    CCTGCTAGCG ACTCTCTCAA CCATAGCCTG TCCTTTATTA GTGATGGGAA CGTTCTGGCT
    CTGCACAGAC TGCTGTGGAA CAATCAAGAA AAAATTGGCC AGTATTTATC TAGCAACAGG
    GATCATAAAG CAGTCGGAAG AAGGCCTTTT GATAAGATGG CCACCCTGCT CGCTTACCTG
    GGTCCCCCAG AACACAAACC TGTGGCAGAT ACTCACTGGT CCAGTTTGAA TCTTACCAGC
    TCCAAATTTG AGGAGTTCAT GACAAGGCAC CAGGTCCATG AGAAAGAAGA GTTCAAAGCA
    TTGAAGACTC TACACATCTT CTACCAGGCT GGAACATCAA AGAACGGAAA CCCTGTGTTT
    TACTATGTGA CGCGCAGGTT CAAGACGGGC CAGATAAATG GGGACTTGCT TATATACCAT
    GTCCTTCTCA CCCTCAAACC ATACTATGCC AAACCCTATG AGCTAGTGGT GGATTTAACC
    CACGCTGGTC CAAGCAACCG CTTCAAGACC GACTTCCTCT CCAAGTGGTT TGTCGTCTTT
    CCGGGATTTG CATATGAAAA CGTTGCTGCC ATTTACATCT ATAACTGTAA CACATGGGTC
    CGGGAATATA CCAAATATCA TGAGAGACTC TTGACGGGAC TCAAAGGCAG TAAGAAGTTA
    CTGTTCATTG ATTCGCCTGC ACGGTTGGCT GAACATGTGG AACCAGAGCA ACAGAAGCTT
    CCGGCAGCTA CACTTGCACT GGAAGAAGAC CTGAAAGTCT TTCACAATGC GCTAAAACTG
    GCCCACAGAG ACACCAAAGT ATCTATTAAG GTGGGCAGCA CAGCAGTGCA GGTCACCTCA
    GCCGAACGAA CACGAGTTTT GGGTCAATCG GTCTTCCTCA ACGACATCTA CTATGCTTCA
    GAGATCGAGG AGATTTGCCT AGTGGACGAA AATCAATTCA CACTGACTAT CGCTAACCAA
    GGGACGCCGC TGACCTTCAT GCACCAGGAG TGTGAGAGCA TAGTTCACTC CATTATCCAC
    ATCCGCACGC GCTGGGAGCT TTCCCAACCT GACTCCATTC CTCAACACAC CAAAATCAGG
    CCCAAAGATG TTCCTGGGAC CCTTCTCAAC ATCGCTCTCC TAAACCTGGG CAGCTCGGAC
    CCCAGTTTAA GGTCTGCAGC TTATAACCTC CTGTGCGCCT TAACATGCAC CTTTAACCTG
    AAGATCGAAG GGCAGCTTCT GGAGACGTCT GGCCTGTGCA TCCCTGCCAA TAACACTCTA
    TTCATCGTGT CCATTAGCCA GACTCTAGCG GCTAATGAGC CTCACCTGAC CCTGGAGTTC
    CTGGAGGAGT GTATCTCTGG ATTCAGCAAG TCCAGTATAG AACTAAAGCA CCTGTGTTTA
    GAGTACATGA CTCCTTGGCT GCTGAACCTG GTACGCTTCT GCAAGCATAG CGATGATGCC
    AAAAGGCAAC GAGTCACCGC CATTCTGGAC AAACTCATTA CCATGACCAT CAACGAAAAG
    CAGATGTATC CATCCATTCA GGCCAAGATC TGGGGGAGCT TGGGCCAGAT AACTGATCTC
    CTTGATGTAG TTCTGGACAG TTTCATTAAA ACCAGTGCGA CAGGAGGTCT CGGATCCATC
    AAAGCTGAAG TGATGGCGGA CACTGCAGTG GCTTTGGCTT CTGGGAATGT CAAATTGGTG
    TCAAGCAAGG TGATCATTCG CATGTGCAAA ATCATCGATA AGACGTGTCT GTCGCCCACA
    CCAACACTGG AGCAGCACCT GATGTGGGAT GATATCGCCA TCCTGGCCCG GTACATGCTC
    ATGCTGTCCT TCAACAACTC TCTGGATGTA GCCGCACACC TGCCGTACCT GTTTCATGTG
    GTGACCCTGC TGGTGGCCAC GGGGCCTCTG TCTCTGCGGG CCTCCACGCA TGGCTTGGTC
    ATCAACATCA TCCACTCCCT CTGCACATGC TCCCAGCTGA GCTTCAGCGA GGAGACCAAG
    CAGGTGTTGC GTCTCAGTCT GACCGAGTTC TCCCTGCCGA AGTTCTACCT GCTGTTCGGC
    ATCAGCAAGG TCAAGTCGGC TGCGGTCATT GCATTTCGTT CCAGCTACAG GGATCGCTCA
    TTCTCGCCGG GGTCCTATGA GAGAGAGACC TTTGCTCTGT CATCTCTGGA GACGGTCACA
    GAGGCGCTGC TGGAGATCAT GGAGGCTTGC ATGAGGGACA TTCCAGGCTG TAAATGGCTA
    GACCAGTGGA CCGATCTGGC GCAGAAGTTT GCATTTCAAT ATAATCCATC CCTCCAACCA
    CGGGCACTTG TGGTATTCGG CTGCATCAGC AAACGAGTCA CACATACGCA GATGAAACAG
    ATCATACGGA TCTTGAGCAA GGCCAGCCTT CTCCTCAGCA TCGACCGGGG TTTGGAGAGC
    TGCCTGAAGG GTCCCGATAA CTGTAACAGC CAGGTGCTAA TCGAAGCCAC AGTCATTGCT
    CTCACTAAAC TACAACCTTT GCTCAACAAG GACTCAGCGA TGCACAAAGC TCTGTTCTGG
    GTCGCTGTGG CCGTACTGCA GTTGGATGAG GTGAATCTGT ATTCTGCAGG AACTGCTCTG
    CTTGAACAGA ACCTACACAC ACTCGATAGC CTGAGAGTCT TCAACGACAA GAGTCCTGAG
    GAGGTGTTTA TGGAGATCCG TCAGCCTCTG GAATGGCACT GTAAACAAAT GGACCACTTT
    GTTGGCCTCA ATTTTAACTC CAACTTCAAT TTTGCTTTGG TCGGTCACCT GTTGAAAGGG
    TTCAGACACC CCTCCCCCAC CACAGTGGCA CGTACCATCC GCATCCTGCA CATGCTGTTG
    GCGTTAGTAG GAAAACACCA GAACTGTGAT AAATTTGAGG TGAACACACA GAGTGTGGCG
    TATCTGGCAG CGCTTCTCCC GGTGTCAGAG GAAGTGCGCA GCCGCTGCAG TCTGAAACAC
    AGAAAATTGT TGCTTTTCTC TGATGTCACA ATGGAGAATG TTCAAATGGA TCCGTATTCT
    GTACATCACG AGGATCCAAG CGGCAGAACG TTGCGGGAAA ACGAGCTGTG GGCATCCCCG
    AAAGTCTCAG AGCGATACTT GGTGGCCCAT TTCCCCATAA TGGGGCAGAT CAGCCCACGA
    ACACGCAAAT CAATGAGCTT GGACATGGGA CAACCCTCTC AAGTCAACAC CAAGAAGCTT
    CTGGGCACCA GAAAAAGCTT TGACCTCCTG TTATCAGACA GCAAAGCACC AAAGAGGCCT
    GAAATAGAGA CTGGTATCAC AACGCCACCC AAGATGAGGA GAGTGGCTGA AAACGAATAT
    GAAATCGAAA CCCCAAGAAT CTCCCATCAC CACTTGCGGA AGGTTTCTGT GTCCGAGTCC
    AATGTCCTGC TTAATGAAGA AGTTCTCACA GATCCCAAAA TCCAAGCACT GCTTCTGACT
    GTGCTGGCCA CTCAGGTGAA AAACACTTCA GATGAATTTG AGCAGAGGAT CTTGTATGAG
    TTTCTAGCCG AGGCCAGTGT TGTCTTCCCA AAAGTCTTTC CTGTTGTTCA TAATCTTCTG
    GACTCTAAGA TTAATACGTT GTTGTCGCTG TGTCAGGACC CACATCTTCT GAATCCAGTT
    CATGGAATTG TTCAGAGTGT AGTTTATCAT GAAGAGTCTC CACCTCAGTA CCAACCGTCC
    TACCTGCAAA CTTTTGGGTT TAATGGACTC TGGAGGTTTG CCGGTCCTTT TTCGAAGCAA
    ACTCAGTTCC CAGACTATGC AGAGCTGATA GTCAAGTTTC TGGATGCACT GATTGACACC
    TATCTTCCGG GCATTGATGA GGAAACAAGC GAGGAGGGTC TGCGGACACC CACCTCTCCA
    TACCCTCCAC CTGTGCAGAG CCAGCTCAGC ATCACTGCCA ACCTCAACCT CTCAAATTCA
    ATGACCTCGC TGGCCACCTC CCAACATGCA CCAGGAATGG ATAAAGAGAA AGTGGATCTA
    TCCCCCACCA CGGGTTTGGC AGCCGGCGGA CGCACACGCC ACGGCTCGGC CAGCCAGGTC
    CAGAAACAGC GCAGCACCGG CAGCTTTAAG AGACCTAGCA TTAAAAAGAT TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169247.1
    CDS265..8481
    Translation

    Target ORF information:

    RefSeq Version XM_005169190.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169190.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
    CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG
    GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC
    ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC
    CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC
    AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC
    ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT
    GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG
    CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT
    CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA
    TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT
    TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA
    GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG
    AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC
    ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG
    GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA
    GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT
    GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA
    GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC
    TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG
    GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC
    ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG
    CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG
    CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG
    AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC
    ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC
    AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA
    GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG
    CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC
    TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG
    CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA
    GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC
    ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC
    CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG
    CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT
    TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA
    AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA
    AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG
    TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT
    CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG
    GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC
    AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC
    AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT
    GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC
    GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG
    GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC
    AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG
    ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC
    AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT
    CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA
    TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG
    CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC
    ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC
    GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG
    ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC
    GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG
    GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT
    CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG
    GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG
    ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG
    AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA
    CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC
    TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT
    CACTGTCTCT TTCAGGTCGT GAGCCAGCGT TTTCCTCAGA ACAGCATTGG AGCTGTAGGC
    AGTGCCATGT TCTTGCGATT CGTGAATCCA GCTATAGTCT CTCCATACGA GGCTGGGATT
    CTTGACAAAA AACCTCAGCC TAGGATAGAA AGAGGCCTAA AACTCATGTC CAAGATTCTC
    CAAAGTATTG CCAACCATGT TTTGTTCACA AAAGAGGAGC ACATGAGGCC TTTCAATGAC
    TTTGTGAAGA GTAATTTTGA TGCTGCCAGA AGATTTTTCC TGGACATCGC CTCTGATTGC
    CCTGCTAGCG ACTCTCTCAA CCATAGCCTG TCCTTTATTA GTGATGGGAA CGTTCTGGCT
    CTGCACAGAC TGCTGTGGAA CAATCAAGAA AAAATTGGCC AGTATTTATC TAGCAACAGG
    GATCATAAAG CAGTCGGAAG AAGGCCTTTT GATAAGATGG CCACCCTGCT CGCTTACCTG
    GGTCCCCCAG AACACAAACC TGTGGCAGAT ACTCACTGGT CCAGTTTGAA TCTTACCAGC
    TCCAAATTTG AGGAGTTCAT GACAAGGCAC CAGGTCCATG AGAAAGAAGA GTTCAAAGCA
    TTGAAGACTC TACACATCTT CTACCAGGCT GGAACATCAA AGAACGGAAA CCCTGTGTTT
    TACTATGTGA CGCGCAGGTT CAAGACGGGC CAGATAAATG GGGACTTGCT TATATACCAT
    GTCCTTCTCA CCCTCAAACC ATACTATGCC AAACCCTATG AGCTAGTGGT GGATTTAACC
    CACGCTGGTC CAAGCAACCG CTTCAAGACC GACTTCCTCT CCAAGTGGTT TGTCGTCTTT
    CCGGGATTTG CATATGAAAA CGTTGCTGCC ATTTACATCT ATAACTGTAA CACATGGGTC
    CGGGAATATA CCAAATATCA TGAGAGACTC TTGACGGGAC TCAAAGGCAG TAAGAAGTTA
    CTGTTCATTG ATTCGCCTGC ACGGTTGGCT GAACATGTGG AACCAGAGCA ACAGAAGCTT
    CCGGCAGCTA CACTTGCACT GGAAGAAGAC CTGAAAGTCT TTCACAATGC GCTAAAACTG
    GCCCACAGAG ACACCAAAGT ATCTATTAAG GTGGGCAGCA CAGCAGTGCA GGTCACCTCA
    GCCGAACGAA CACGAGTTTT GGGTCAATCG GTCTTCCTCA ACGACATCTA CTATGCTTCA
    GAGATCGAGG AGATTTGCCT AGTGGACGAA AATCAATTCA CACTGACTAT CGCTAACCAA
    GGGACGCCGC TGACCTTCAT GCACCAGGAG TGTGAGAGCA TAGTTCACTC CATTATCCAC
    ATCCGCACGC GCTGGGAGCT TTCCCAACCT GACTCCATTC CTCAACACAC CAAAATCAGG
    CCCAAAGATG TTCCTGGGAC CCTTCTCAAC ATCGCTCTCC TAAACCTGGG CAGCTCGGAC
    CCCAGTTTAA GGTCTGCAGC TTATAACCTC CTGTGCGCCT TAACATGCAC CTTTAACCTG
    AAGATCGAAG GGCAGCTTCT GGAGACGTCT GGCCTGTGCA TCCCTGCCAA TAACACTCTA
    TTCATCGTGT CCATTAGCCA GACTCTAGCG GCTAATGAGC CTCACCTGAC CCTGGAGTTC
    CTGGAGGAGT GTATCTCTGG ATTCAGCAAG TCCAGTATAG AACTAAAGCA CCTGTGTTTA
    GAGTACATGA CTCCTTGGCT GCTGAACCTG GTACGCTTCT GCAAGCATAG CGATGATGCC
    AAAAGGCAAC GAGTCACCGC CATTCTGGAC AAACTCATTA CCATGACCAT CAACGAAAAG
    CAGATGTATC CATCCATTCA GGCCAAGATC TGGGGGAGCT TGGGCCAGAT AACTGATCTC
    CTTGATGTAG TTCTGGACAG TTTCATTAAA ACCAGTGCGA CAGGAGGTCT CGGATCCATC
    AAAGCTGAAG TGATGGCGGA CACTGCAGTG GCTTTGGCTT CTGGGAATGT CAAATTGGTG
    TCAAGCAAGG TGATCATTCG CATGTGCAAA ATCATCGATA AGACGTGTCT GTCGCCCACA
    CCAACACTGG AGCAGCACCT GATGTGGGAT GATATCGCCA TCCTGGCCCG GTACATGCTC
    ATGCTGTCCT TCAACAACTC TCTGGATGTA GCCGCACACC TGCCGTACCT GTTTCATGTG
    GTGACCCTGC TGGTGGCCAC GGGGCCTCTG TCTCTGCGGG CCTCCACGCA TGGCTTGGTC
    ATCAACATCA TCCACTCCCT CTGCACATGC TCCCAGCTGA GCTTCAGCGA GGAGACCAAG
    CAGGTGTTGC GTCTCAGTCT GACCGAGTTC TCCCTGCCGA AGTTCTACCT GCTGTTCGGC
    ATCAGCAAGG TCAAGTCGGC TGCGGTCATT GCATTTCGTT CCAGCTACAG GGATCGCTCA
    TTCTCGCCGG GGTCCTATGA GAGAGAGACC TTTGCTCTGT CATCTCTGGA GACGGTCACA
    GAGGCGCTGC TGGAGATCAT GGAGGCTTGC ATGAGGGACA TTCCAGGCTG TAAATGGCTA
    GACCAGTGGA CCGATCTGGC GCAGAAGTTT GCATTTCAAT ATAATCCATC CCTCCAACCA
    CGGGCACTTG TGGTATTCGG CTGCATCAGC AAACGAGTCA CACATACGCA GATGAAACAG
    ATCATACGGA TCTTGAGCAA GGCCAGCCTT CTCCTCAGCA TCGACCGGGG TTTGGAGAGC
    TGCCTGAAGG GTCCCGATAA CTGTAACAGC CAGGTGCTAA TCGAAGCCAC AGTCATTGCT
    CTCACTAAAC TACAACCTTT GCTCAACAAG GACTCAGCGA TGCACAAAGC TCTGTTCTGG
    GTCGCTGTGG CCGTACTGCA GTTGGATGAG GTGAATCTGT ATTCTGCAGG AACTGCTCTG
    CTTGAACAGA ACCTACACAC ACTCGATAGC CTGAGAGTCT TCAACGACAA GAGTCCTGAG
    GAGGTGTTTA TGGAGATCCG TCAGCCTCTG GAATGGCACT GTAAACAAAT GGACCACTTT
    GTTGGCCTCA ATTTTAACTC CAACTTCAAT TTTGCTTTGG TCGGTCACCT GTTGAAAGGG
    TTCAGACACC CCTCCCCCAC CACAGTGGCA CGTACCATCC GCATCCTGCA CATGCTGTTG
    GCGTTAGTAG GAAAACACCA GAACTGTGAT AAATTTGAGG TGAACACACA GAGTGTGGCG
    TATCTGGCAG CGCTTCTCCC GGTGTCAGAG GAAGTGCGCA GCCGCTGCAG TCTGAAACAC
    AGAAAATTGT TGCTTTTCTC TGATGTCACA ATGGAGAATG TTCAAATGGA TCCGTATTCT
    GTACATCACG AGGATCCAAG CGGCAGAACG TTGCGGGAAA ACGAGCTGTG GGCATCCCCG
    AAAGTCTCAG AGCGATACTT GGTGGCCCAT TTCCCCATAA TGGGGCAGAT CAGCCCACGA
    ACACGCAAAT CAATGAGCTT GGACATGGGA CAACCCTCTC AAGTCAACAC CAAGAAGCTT
    CTGGGCACCA GAAAAAGCTT TGACCTCCTG TTATCAGACA GCAAAGCACC AAAGAGGCCT
    GAAATAGAGA CTGGTATCAC AACGCCACCC AAGATGAGGA GAGTGGCTGA AAACGAATAT
    GAAATCGAAA CCCCAAGAAT CTCCCATCAC CACTTGCGGA AGGTTTCTGT GTCCGAGTCC
    AATGTCCTGC TTAATGAAGA AGTTCTCACA GATCCCAAAA TCCAAGCACT GCTTCTGACT
    GTGCTGGCCA CTCAGGTGAA AAACACTTCA GATGAATTTG AGCAGAGGAT CTTGTATGAG
    TTTCTAGCCG AGGCCAGTGT TGTCTTCCCA AAAGTCTTTC CTGTTGTTCA TAATCTTCTG
    GACTCTAAGA TTAATACGTT GTTGTCGCTG TGTCAGGACC CACATCTTCT GAATCCAGTT
    CATGGAATTG TTCAGAGTGT AGTTTATCAT GAAGAGTCTC CACCTCAGTA CCAACCGTCC
    TACCTGCAAA CTTTTGGGTT TAATGGACTC TGGAGGTTTG CCGGTCCTTT TTCGAAGCAA
    ACTCAGTTCC CAGACTATGC AGAGCTGATA GTCAAGTTTC TGGATGCACT GATTGACACC
    TATCTTCCGG GCATTGATGA GGAAACAAGC GAGGAGGGTC TGCGGACACC CACCTCTCCA
    TACCCTCCAC CTGTGCAGAG CCAGCTCAGC ATCACTGCCA ACCTCAACCT CTCAAATTCA
    ATGACCTCGC TGGCCACCTC CCAACATGCA CCAGGAATGG ATAAAGAGAA AGTGGATCTA
    TCCCCCACCA CGGGTTTGGC AGCCGGCGGA CGCACACGCC ACGGCTCGGC CAGCCAGGTC
    CAGAAACAGC GCAGCACCGG CAGCTTTAAG AGACCTAGCA TTAAAAAGAT TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.