dgcr8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dgcr8
Entrez Gene ID	563963
Full Name	DGCR8 microprocessor complex subunit
Synonyms	si:ch211-106a19.4,wu:fc23f08,wu:fc38f06
General protein information	Preferred Names DGCR8 microprocessor complex subunit Names microprocessor complex subunit DGCR8 DiGeorge syndrome critical region gene 8
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001122749.1	NP_001116221.1	microprocessor complex subunit DGCR8

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dgcr8	NP_201581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002554	XP_312385.5
Homo sapiens (human)	DGCR8	NP_073557.3
Bos taurus (cattle)	DGCR8	NP_001094674.1
Rattus norvegicus (Norway rat)	Dgcr8	NP_001099335.1
Drosophila melanogaster (fruit fly)	pasha	NP_001263150.1
Pan troglodytes (chimpanzee)	DGCR8	XP_003953874.1
	DGCR8	XP_002798321.1
Canis lupus familiaris (dog)	DGCR8	XP_543542.4
Gallus gallus (chicken)	DGCR8	XP_415079.2
Danio rerio (zebrafish)	dgcr8	NP_001116221.1
Xenopus tropicalis (tropical clawed frog)	dgcr8	NP_001153011.1

Gene Ontology
Bibliography

Related articles in PubMed

Germline-specific dgcr8 knockout in zebrafish using a BACK approach.
Liu Y, Zhu Z, Ho IHT, Shi Y, Xie Y, Li J, Zhang Y, Chan MTV, Cheng CHK
Cellular and molecular life sciences : CMLS74(13)2503-2511(2017 Jul)

Lin CY, He JY, Zeng CW, Loo MR, Chang WY, Zhang PH, Tsai HJ
Open biology7(7)(2017 Jul)

Comparative mapping of the 22q11.2 deletion region and the potential of simple model organisms.
Guna A, Butcher NJ, Bassett AS
Journal of neurodevelopmental disorders7(1)18(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DiGeorge syndrome critical region gene 8 (dgcr8) in zebrafish germ line was deleted and demonstrated that the maternal-zygotic dgcr8 (MZdgcr8) embryos exhibit MZdicer-like phenotypes with morphological defects which could be rescued by miR-430.
Title: Germline-specific dgcr8 knockout in zebrafish using a BACK approach.

The following dgcr8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgcr8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01055	NM_001122749.1 Latest version!	Danio rerio DGCR8 microprocessor complex subunit (dgcr8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dgcr8 ( NM_001122749.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116221.1 Danio rerio DGCR8 microprocessor complex subunit (dgcr8), mRNA.

Price & Availability↑

CloneID	ODa01055
Clone ID Related Accession (Same CDS sequence)	NM_001122749.1
Accession Version	NM_001122749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	microprocessor complex subunit DGCR8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX088551.7. On Mar 19, 2008 this sequence version replaced XM_687324.2. ##Evidence-Data-START## CDS exon combination :: GFIL01008563.1, GDQQ01001169.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGATGTAA ATGAGATTTT ACCTCCCCTC CCATTGGAGC CACCTGACGC AGTATTGGCG 
GGGAATGATT ACAGTAAAGC ACCTCCACCA CCTCCCCTGC AAACGTCCAG TGACGCAGAG 
GAAATGGACG TTAGCTCTGG TGGTGATGGA CACCAACACA CCCCAGCAGT GGACAGGGAC 
GAACAGCTTC TCCTCAACAA ACGCACATTG TCTGTTAGTA GCCGCCCATC AGATAGTACT 
GACCCCATGA CTACTTGCCC TAGAACCGCT CGCCATGCTC CCCCTGTTAC CAAGTTTTTA 
CCTGATCTCA AACTCCTCAG AGACGTAAAG ATCAGTGTCA GCTTTTCGGA CAGCAGTAAG 
AGCAAAGACA GGAAGGTGTT GTACACTGGT TTTGGGCAGG TTAGCGATGA AGATCAGAGC 
CTAGAGAGCA TAAACGGAGA ATTGCATGAT TCTGTAATGA TGAGAAGCGT GCCTGGCAGT 
TCAGCTATGA GCACTGGCTT CATGGGTTTA GCTAGAACTA GAGAAGAGCT GGAGGCAGAG 
CAGGAGAACA AGGTTGAGTT CGCTGTGCTG GATGACCTGG AAGATTTCAG CGAGAACTTT 
CTGGAAGATG GAGAGCCAAG TGCTTTCAGG TCTCAGATGA TAGTTCAGCA GGAGCAAGTG 
CACGAGGAGG ACCTGAGTTA CTCCTATGAG GAGGACTTTG ACAATGATGT AGATGCCCTG 
TTGGAGGAAG GCATGCCCAT GCCCAAGAAG AGGCGTGTGG CTGAAGACAA ATATGCTGGA 
GACAGTGATC ACCATTCAGA CGGGGAGGCC GGAGTTCAGC CTATGATGAC CAAAATTAAG 
ACCATTCTTA AGAGTCGTGG TCGACCGCCA ACTGAGCCAT TACCTGATGG GTGGATCATG 
ACATTCCATA ACTCCGGCAT TCCAGTCTAC CTCCACCGGG AAACCAGAGT TGTAACCTGG 
TCTAGACCTT ACTTCCTTGG AACAGGAAGC ATTAGGAAAC ATGACCCACC AACCAGTAGC 
ATTCCCTGCC TGCACTACAA GAAAATGAAG GAACAAGAAG AAAGGGAACA GAATGGAGAG 
GTGACTCCGA CAGCGGAGAT TTCTCCAGTC AAATCTGGGG AGGAGGGTAT CTCACTGGAG 
CGAGCCGATG AGCCTGACTC CACTGCCACT GAAGAGCCCA CCGGCAGACC AACATCAGAA 
GATCCGGATC TTGACCTCAT GGAGCCAGGA GGGTCTTCTA TAGAGGGGAA GGATGGCCAA 
ACCGGGGATA CTGCACAGGG AGCATTGGGC CAGGTCAAAG CCAAGGTGGA GGTCTGCAAA 
GATGAGTCTA TTGAAATTGA GGATTTTAGA AGCTACCTAG AGAAGTGCTT TGATTTTGAG 
CAAGTGACAG TGAAGAAGTT CCGCACATGG GCTGAGCGCA GACAGTTCAA CAGGGACATG 
AAGAGGAAAC AGGCTGAGTC TGAGAGGCCC ATCCTACCAG CCAATCAGAA GCTCATCACG 
CTGTCTGTTC AAGATGCCCC CACAAAGAAA GAGTTTGTCA TCAACCCAAA TGGGAAGTCA 
GAAGTGTGCA TTTTACATGA GTATATGCAA AGAGTACTCA AAGTGCGACC AGTTTACAAC 
TTTTTTGAAT GTGAAAACCC AAGTGAACCC TTCGGAGCCT CAGTGATTAT AGACGGTGTT 
ACATACGGCA CTGGAACAGC TAGTAGTAAA AAACTTGCCA AGAATAAAGC TGCTCGAGCA 
ACACTGGAAA TCCTCATTCC TGACTTTGTG AAGCAGACGT CTGAGGAGAA GCCCGTAGAG 
GGAGATGAAC TGGAGTATTT TAATCATATC AGTATTGAAG ATTCAAGGGT GTATGAGCTG 
ACCAACAAAG CAGGTTTACT CTCACCATAT CAGATCCTTC ATGAGTGCCT TAAGAGAAAC 
CATGGCATGG GAGACACCAG CATCAAGTTT GAGGTGATTC CAGGAAAGAA CCAGAAGAGT 
GAATACGTCA TGACATGTGG GAAGCACACT GTGCGCGGCT GGTGTAAAAA TAAGCGAGTC 
GGAAAGCAGC TGGCGTCACA GAAGATTCTC CAGATGCTTC ATCCTCATGT GAAGAACTGG 
GGCTCACTGC TGCGCATGTA TGGCAGAGAA AGCAACAAAA TGGTTAAAAA GGAGAATTCT 
GATAAGAGTG TGATTGAGCT TCAGCAGTAT GCCAAAAAGA ACAAGCCAAA CCTTCACATC 
CTCAACAAAC TGCGAGAGGA AATGATGAAA CTGGCTCGAG AAAGGGAGGA GGCAAGAAAA 
AAGCCGAAGA TGACTATAAT GGAGTCTGCT CAACCTGGCA GTGAACCTCT GTGTACTGTT 
GACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116221.1
CDS	423..2771
Translation	MDVNEILPPLPLEPPDAVLAGNDYSKAPPPPPLQTSSDAEEMDVSSGGDGHQHTPAVDRDEQLLLNKRTLSVSSRPSDSTDPMTTCPRTARHAPPVTKFLPDLKLLRDVKISVSFSDSSKSKDRKVLYTGFGQVSDEDQSLESINGELHDSVMMRSVPGSSAMSTGFMGLARTREELEAEQENKVEFAVLDDLEDFSENFLEDGEPSAFRSQMIVQQEQVHEEDLSYSYEEDFDNDVDALLEEGMPMPKKRRVAEDKYAGDSDHHSDGEAGVQPMMTKIKTILKSRGRPPTEPLPDGWIMTFHNSGIPVYLHRETRVVTWSRPYFLGTGSIRKHDPPTSSIPCLHYKKMKEQEEREQNGEVTPTAEISPVKSGEEGISLERADEPDSTATEEPTGRPTSEDPDLDLMEPGGSSIEGKDGQTGDTAQGALGQVKAKVEVCKDESIEIEDFRSYLEKCFDFEQVTVKKFRTWAERRQFNRDMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVIIDGVTYGTGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPVEGDELEYFNHISIEDSRVYELTNKAGLLSPYQILHECLKRNHGMGDTSIKFEVIPGKNQKSEYVMTCGKHTVRGWCKNKRVGKQLASQKILQMLHPHVKNWGSLLRMYGRESNKMVKKENSDKSVIELQQYAKKNKPNLHILNKLREEMMKLAREREEARKKPKMTIMESAQPGSEPLCTVDV

Target ORF information:

RefSeq Version	NM_001122749.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DGCR8 microprocessor complex subunit (dgcr8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122749.1

ORF Insert Sequence:

ATGGATGTAA ATGAGATTTT ACCTCCCCTC CCATTGGAGC CACCTGACGC AGTATTGGCG 
GGGAATGATT ACAGTAAAGC ACCTCCACCA CCTCCCCTGC AAACGTCCAG TGACGCAGAG 
GAAATGGACG TTAGCTCTGG TGGTGATGGA CACCAACACA CCCCAGCAGT GGACAGGGAC 
GAACAGCTTC TCCTCAACAA ACGCACATTG TCTGTTAGTA GCCGCCCATC AGATAGTACT 
GACCCCATGA CTACTTGCCC TAGAACCGCT CGCCATGCTC CCCCTGTTAC CAAGTTTTTA 
CCTGATCTCA AACTCCTCAG AGACGTAAAG ATCAGTGTCA GCTTTTCGGA CAGCAGTAAG 
AGCAAAGACA GGAAGGTGTT GTACACTGGT TTTGGGCAGG TTAGCGATGA AGATCAGAGC 
CTAGAGAGCA TAAACGGAGA ATTGCATGAT TCTGTAATGA TGAGAAGCGT GCCTGGCAGT 
TCAGCTATGA GCACTGGCTT CATGGGTTTA GCTAGAACTA GAGAAGAGCT GGAGGCAGAG 
CAGGAGAACA AGGTTGAGTT CGCTGTGCTG GATGACCTGG AAGATTTCAG CGAGAACTTT 
CTGGAAGATG GAGAGCCAAG TGCTTTCAGG TCTCAGATGA TAGTTCAGCA GGAGCAAGTG 
CACGAGGAGG ACCTGAGTTA CTCCTATGAG GAGGACTTTG ACAATGATGT AGATGCCCTG 
TTGGAGGAAG GCATGCCCAT GCCCAAGAAG AGGCGTGTGG CTGAAGACAA ATATGCTGGA 
GACAGTGATC ACCATTCAGA CGGGGAGGCC GGAGTTCAGC CTATGATGAC CAAAATTAAG 
ACCATTCTTA AGAGTCGTGG TCGACCGCCA ACTGAGCCAT TACCTGATGG GTGGATCATG 
ACATTCCATA ACTCCGGCAT TCCAGTCTAC CTCCACCGGG AAACCAGAGT TGTAACCTGG 
TCTAGACCTT ACTTCCTTGG AACAGGAAGC ATTAGGAAAC ATGACCCACC AACCAGTAGC 
ATTCCCTGCC TGCACTACAA GAAAATGAAG GAACAAGAAG AAAGGGAACA GAATGGAGAG 
GTGACTCCGA CAGCGGAGAT TTCTCCAGTC AAATCTGGGG AGGAGGGTAT CTCACTGGAG 
CGAGCCGATG AGCCTGACTC CACTGCCACT GAAGAGCCCA CCGGCAGACC AACATCAGAA 
GATCCGGATC TTGACCTCAT GGAGCCAGGA GGGTCTTCTA TAGAGGGGAA GGATGGCCAA 
ACCGGGGATA CTGCACAGGG AGCATTGGGC CAGGTCAAAG CCAAGGTGGA GGTCTGCAAA 
GATGAGTCTA TTGAAATTGA GGATTTTAGA AGCTACCTAG AGAAGTGCTT TGATTTTGAG 
CAAGTGACAG TGAAGAAGTT CCGCACATGG GCTGAGCGCA GACAGTTCAA CAGGGACATG 
AAGAGGAAAC AGGCTGAGTC TGAGAGGCCC ATCCTACCAG CCAATCAGAA GCTCATCACG 
CTGTCTGTTC AAGATGCCCC CACAAAGAAA GAGTTTGTCA TCAACCCAAA TGGGAAGTCA 
GAAGTGTGCA TTTTACATGA GTATATGCAA AGAGTACTCA AAGTGCGACC AGTTTACAAC 
TTTTTTGAAT GTGAAAACCC AAGTGAACCC TTCGGAGCCT CAGTGATTAT AGACGGTGTT 
ACATACGGCA CTGGAACAGC TAGTAGTAAA AAACTTGCCA AGAATAAAGC TGCTCGAGCA 
ACACTGGAAA TCCTCATTCC TGACTTTGTG AAGCAGACGT CTGAGGAGAA GCCCGTAGAG 
GGAGATGAAC TGGAGTATTT TAATCATATC AGTATTGAAG ATTCAAGGGT GTATGAGCTG 
ACCAACAAAG CAGGTTTACT CTCACCATAT CAGATCCTTC ATGAGTGCCT TAAGAGAAAC 
CATGGCATGG GAGACACCAG CATCAAGTTT GAGGTGATTC CAGGAAAGAA CCAGAAGAGT 
GAATACGTCA TGACATGTGG GAAGCACACT GTGCGCGGCT GGTGTAAAAA TAAGCGAGTC 
GGAAAGCAGC TGGCGTCACA GAAGATTCTC CAGATGCTTC ATCCTCATGT GAAGAACTGG 
GGCTCACTGC TGCGCATGTA TGGCAGAGAA AGCAACAAAA TGGTTAAAAA GGAGAATTCT 
GATAAGAGTG TGATTGAGCT TCAGCAGTAT GCCAAAAAGA ACAAGCCAAA CCTTCACATC 
CTCAACAAAC TGCGAGAGGA AATGATGAAA CTGGCTCGAG AAAGGGAGGA GGCAAGAAAA 
AAGCCGAAGA TGACTATAAT GGAGTCTGCT CAACCTGGCA GTGAACCTCT GTGTACTGTT 
GACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Germline-specific dgcr8 knockout in zebrafish using a BACK approach.
Cellular and molecular life sciences : CMLS74(13)2503-2511(2017 Jul)
Liu Y,Zhu Z,Ho IHT,Shi Y,Xie Y,Li J,Zhang Y,Chan MTV,Cheng CHK

Open biology7(7)(2017 Jul)
Lin CY,He JY,Zeng CW,Loo MR,Chang WY,Zhang PH,Tsai HJ

Comparative mapping of the 22q11.2 deletion region and the potential of simple model organisms.
Journal of neurodevelopmental disorders7(1)18(2015)
Guna A,Butcher NJ,Bassett AS