pcnx4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pcnx4
Entrez Gene ID	563650
Full Name	pecanex-like 4 (Drosophila)
Synonyms	pcnx4,zgc:158219
General protein information	Preferred Names pecanex-like 4 (Drosophila) Names pecanex-like protein 4 pecanex homolog protein 4 pecanex-like protein C14orf135 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307332.3	XP_009305607.1	pecanex-like protein 4 isoform X1
XM_009307333.3	XP_009305608.1	pecanex-like protein 4 isoform X2
XM_009307332.2	XP_009305607.1	pecanex-like protein 4 isoform X1
XM_009307333.2	XP_009305608.1	pecanex-like protein 4 isoform X2
NM_001081574.1	NP_001075043.1	pecanex-like protein 4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pcnxl4	NP_080603.2
Danio rerio (zebrafish)	pcnxl4	NP_001075043.1
Xenopus tropicalis (tropical clawed frog)	pcnxl4	NP_001121514.1
Canis lupus familiaris (dog)	PCNXL4	XP_537464.3
Gallus gallus (chicken)	C5H14ORF135	XP_421424.4
Pan troglodytes (chimpanzee)	PCNXL4	XP_003954462.1
Bos taurus (cattle)	PCNXL4	XP_003586633.1
Rattus norvegicus (Norway rat)	Pcnxl4	XP_002729698.3
Homo sapiens (human)	PCNXL4	NP_071940.4
	PCNXL4	XP_001095482.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Genetic association analyses highlight biological pathways underlying mitral valve prolapse.
Dina C, Bouatia-Naji N, Tucker N, Delling FN, Toomer K, Durst R, Perrocheau M, Fernandez-Friera L, Solis J, , Le Tourneau T, Chen MH, Probst V, Bosse Y, Pibarot P, Zelenika D, Lathrop M, Hercberg S, Roussel R, Benjamin EJ, Bonnet F, Lo SH, Dolmatova E, Simonet F, Lecointe S, Kyndt F, Redon R, Le Marec H, Froguel P, Ellinor PT, Vasan RS, Bruneval P, Markwald RR, Norris RA, Milan DJ, Slaugenhaupt SA, Levine RA, Schott JJ, Hagege AA, , Jeunemaitre X,
Nature genetics47(10)1206-11(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pcnx4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pcnx4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44128	XM_009307333.3 Latest version!	Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa37983	NM_001081574.1 Latest version!	Danio rerio pecanex 4 (pcnx4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa37983	XM_009307332.3 Latest version!	Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa37983	XM_009307332.2	Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa44128	XM_009307333.2	Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pcnx4 ( XM_009307333.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305608.1 Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44128
Clone ID Related Accession (Same CDS sequence)	XM_009307333.3 , XM_009307333.2
Accession Version	XM_009307333.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pecanex-like protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTGT CATCAGTGTG TGTTGCAATC TGCACCTACT TCATTCCCAC TTCACTCGGT 
GTGGTTCTGT TCACCGTGGC CATGGGTTTC CTCTTCAGCC AGGACCTGAC CCAGCTGCTG 
GTGCTGGTAA AAGGGGTCTC TCCGACCCTG GGACTGGGCT GGTGGGGGCT TCCTCTTTCC 
CTGCTCTTCA TAATAGGAGC CTTGACCGAG GCTGGACTTC TTCATCAGCT GAACCTTGTC 
AACTCAGGGA ATCTAACATT GAATACGAAC TCTGTAGAGA ACTGGGAGTC TTCCGGTGTG 
GTTCCTCCAA ACCCACAGGA GGTGCTTGGC TGGCTTCTAA TTGCTCTGTT TTTCATCACT 
CGTCTGCTGA GAGAGCTTCA GGGGGCTTGC ATTATTGGAG GAGCCATGCT CAACCCACTC 
TACCCCAAAC GGGTCACAAC TGCACAGGCT TTCAGGAGGA AAACCCGTGG CCTGCGTGTT 
GCAGGAATCA TTCGGAGGAT CCTATTGAAC CTTGTAAGTC CGTTGACGAT GATTGCGTTC 
TTGTCCGTGG ATGAATCTTT AGGGAAGATA CACACAGCCT CTCTTGCCAT TGGCTTCACT 
CGGGCATTCA GGATGGTGTG GCAGAGCTCT GAGGCGGCTC TACTGCAGAT GGTGTTGGTG 
GTGATCATCA AACTTGCAGG GGGACACAGA GTTGCACACT GGCATGACTT GGGCACTACA 
GTACAGTTAA TACTGGTCTC TCTGGTTGTG AATCGTGTCT CACAGTTTGT ATGTAAGCTG 
AGTTTTGCTC TTACGGTGTT GATCACATCC TGGACTGAGA GTAAACAGCG TCGACAGTCA 
GCAGGAACTC TGCTGGCACT CAATGCTGCT TTGTGCCCAC TTCTACTGGC CGTCGTGGCT 
CTATCTGCTC TTCTCTCTGC CCCTCTCCTT CCACTATTCA CACTACCGGT GTTCCTGGTG 
GGATTTCCCA GGCCGCTGCG CTCATGGCCA GGCACTCCTG GCACCGCCTG CCCTTGCCCA 
GATTCTGTGT ATTACCAGCA GCTCTGCAAA AGGCTGGCAT CAGCTATAAG ACCTGCACTT 
GCTAATGGTG CATTTGGATC CTGCACTCCA GGGTCTCATT ACCTAGGCCG CTTTCAGGGT 
CGTCTTCTGT GGATCTCGAT TCTAGAAAAG GGCTATGGCT ATTGCACAGT CAACATTAAG 
GGTCTGGAAC TACAGGAGAC ATCATGCCAC ACAGTGGAAG CTCGGCGTGT GGACGAGGTT 
TTCGAAGGAG CGTTTGATCA CGTGGAGCGG CCAGGCCAGT GTTTCAGACT ATTGAACCCT 
AACTGGGGTA ACGCTCTTAT CCCCTGCTCT CTGTTGCCCG TCAGGGTTTA CTCTGATGCC 
CAGAGCGTTC TAACGGGAAT TATTGACTCA CCCGATCACC TGCGTCAGCT AAACTCTGAC 
TTCCTCAAAA CACTTGTCTG GATTCTATTG CGTTACTGTG TACAGGAATT CACATGCGGT 
GCTCAGCGAA CTAAAGGTCA GACTTTACAG ACCTCTGGAC GTAAGTCACA AAGCTCCCAG 
CATCCTGTGC CCTCAGAGGC AGGTCCAGCT GTGATCAAAG TGGAGACTCA AGTACGCCCT 
GAATCCTCCT CGTCCTCTCA CTTGAGGGGT CTGGACAGCT CCAGCCTGTC GTCCTTTGCT 
GATTGGTCAG ACGAGGATGA TCTGTTTGGT CCAGTCCCTG TGCGACGACC GATGAGAATG 
ATGGTGCGGA CAGATGAAGC GCAGACAGAG TTAGGGCTGG GTGTGTCTCT TCCAGGTTCT 
GTCGAAATCC ACAGCTTGTA TGAGAGCATG GCTCTGTCTT CTCTACCGAC CCTTAGACCT 
CTGGGTCTGG GTTTGGGACT TGGCCTGCCC ATTATAGATA AAGGGAAAGA AAACATCCCT 
GCACCTACTT CGACAACTGT CCGGACCAAC TCCCAGCCTC TTCTCTTTAC CAGCCCTCAC 
TCAGAGCTCT TCTCTCTGCC CGCAGACTGG CGAACCGCCA ACTTGGCTTC CTCTAAACTC 
CAGGTTTTGC GACCCTGCTT CCCAGAGGCC TGGTTCTGCT TCTGTTTATC CCAGCTGGTG 
CTAGAGAGTT TGGGAGAAGG AGACTTTGAG CAGGTGACGC AGGGTTTACA GGAGGACCGT 
GCCTTGCAAG AGCTTTATAC CCAGGTGGTG CTATCATGCT GTGTGGCGCT AGGGGCAGAT 
GCTCAGGTCT TCAGCCCAAA TCTGCTGTTC AGGCTGTACT GTGGGGACGG CCCCTGGACT 
GAGGGGCTTG AGTGGCTCCG AGCCAATAAA GAGCTGCATC AGCTCACACT CAGAGCTTTC 
AGGTACAGCG TAAAACTATT GGTAGACCAG GCATCTCTTG GTCCAGTAGA GAGTCTGGAT 
GAGCTCTACA CTACTTTGCA GGATTACCAT AAAAACTGGT TCATCGGCCT GGTATCAGAT 
CGCAGTTGGC AAGATAGTGT TGTACAGGAG AAGCCCTTCC TGTTCTCTCT TGGACATGAC 
CTCACCATGG GGACGTACAC CAGCCGTGTG TTGTCCCTGC AGGAGAATCT GGTCCAAGTG 
GGAGCTCTAA ATGAGGAGGG GGTGAGGGGC CAGTGGGCCA ACCTGTCATG GGAACTATTG 
TATGCCACTA ATGACGATGA GGAGCGCTAC AGCATTCAGG CCCATCCAGT GATGCTGAGG 
AACCTCACTA TTCAAGCAGC AGATCCGCCT CTGGGATATC CCATATACTC ATCCCCTCCA 
ATTCACCTCC GCTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305608.1
CDS	87..2867
Translation	MFLSSVCVAICTYFIPTSLGVVLFTVAMGFLFSQDLTQLLVLVKGVSPTLGLGWWGLPLSLLFIIGALTEAGLLHQLNLVNSGNLTLNTNSVENWESSGVVPPNPQEVLGWLLIALFFITRLLRELQGACIIGGAMLNPLYPKRVTTAQAFRRKTRGLRVAGIIRRILLNLVSPLTMIAFLSVDESLGKIHTASLAIGFTRAFRMVWQSSEAALLQMVLVVIIKLAGGHRVAHWHDLGTTVQLILVSLVVNRVSQFVCKLSFALTVLITSWTESKQRRQSAGTLLALNAALCPLLLAVVALSALLSAPLLPLFTLPVFLVGFPRPLRSWPGTPGTACPCPDSVYYQQLCKRLASAIRPALANGAFGSCTPGSHYLGRFQGRLLWISILEKGYGYCTVNIKGLELQETSCHTVEARRVDEVFEGAFDHVERPGQCFRLLNPNWGNALIPCSLLPVRVYSDAQSVLTGIIDSPDHLRQLNSDFLKTLVWILLRYCVQEFTCGAQRTKGQTLQTSGRKSQSSQHPVPSEAGPAVIKVETQVRPESSSSSHLRGLDSSSLSSFADWSDEDDLFGPVPVRRPMRMMVRTDEAQTELGLGVSLPGSVEIHSLYESMALSSLPTLRPLGLGLGLGLPIIDKGKENIPAPTSTTVRTNSQPLLFTSPHSELFSLPADWRTANLASSKLQVLRPCFPEAWFCFCLSQLVLESLGEGDFEQVTQGLQEDRALQELYTQVVLSCCVALGADAQVFSPNLLFRLYCGDGPWTEGLEWLRANKELHQLTLRAFRYSVKLLVDQASLGPVESLDELYTTLQDYHKNWFIGLVSDRSWQDSVVQEKPFLFSLGHDLTMGTYTSRVLSLQENLVQVGALNEEGVRGQWANLSWELLYATNDDEERYSIQAHPVMLRNLTIQAADPPLGYPIYSSPPIHLRCL

Target ORF information:

RefSeq Version	XM_009307333.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307333.3

ORF Insert Sequence:

ATGTTTTTGT CATCAGTGTG TGTTGCAATC TGCACCTACT TCATTCCCAC TTCACTCGGT 
GTGGTTCTGT TCACCGTGGC CATGGGTTTC CTCTTCAGCC AGGACCTGAC CCAGCTGCTG 
GTGCTGGTAA AAGGGGTCTC TCCGACCCTG GGACTGGGCT GGTGGGGGCT TCCTCTTTCC 
CTGCTCTTCA TAATAGGAGC CTTGACCGAG GCTGGACTTC TTCATCAGCT GAACCTTGTC 
AACTCAGGGA ATCTAACATT GAATACGAAC TCTGTAGAGA ACTGGGAGTC TTCCGGTGTG 
GTTCCTCCAA ACCCACAGGA GGTGCTTGGC TGGCTTCTAA TTGCTCTGTT TTTCATCACT 
CGTCTGCTGA GAGAGCTTCA GGGGGCTTGC ATTATTGGAG GAGCCATGCT CAACCCACTC 
TACCCCAAAC GGGTCACAAC TGCACAGGCT TTCAGGAGGA AAACCCGTGG CCTGCGTGTT 
GCAGGAATCA TTCGGAGGAT CCTATTGAAC CTTGTAAGTC CGTTGACGAT GATTGCGTTC 
TTGTCCGTGG ATGAATCTTT AGGGAAGATA CACACAGCCT CTCTTGCCAT TGGCTTCACT 
CGGGCATTCA GGATGGTGTG GCAGAGCTCT GAGGCGGCTC TACTGCAGAT GGTGTTGGTG 
GTGATCATCA AACTTGCAGG GGGACACAGA GTTGCACACT GGCATGACTT GGGCACTACA 
GTACAGTTAA TACTGGTCTC TCTGGTTGTG AATCGTGTCT CACAGTTTGT ATGTAAGCTG 
AGTTTTGCTC TTACGGTGTT GATCACATCC TGGACTGAGA GTAAACAGCG TCGACAGTCA 
GCAGGAACTC TGCTGGCACT CAATGCTGCT TTGTGCCCAC TTCTACTGGC CGTCGTGGCT 
CTATCTGCTC TTCTCTCTGC CCCTCTCCTT CCACTATTCA CACTACCGGT GTTCCTGGTG 
GGATTTCCCA GGCCGCTGCG CTCATGGCCA GGCACTCCTG GCACCGCCTG CCCTTGCCCA 
GATTCTGTGT ATTACCAGCA GCTCTGCAAA AGGCTGGCAT CAGCTATAAG ACCTGCACTT 
GCTAATGGTG CATTTGGATC CTGCACTCCA GGGTCTCATT ACCTAGGCCG CTTTCAGGGT 
CGTCTTCTGT GGATCTCGAT TCTAGAAAAG GGCTATGGCT ATTGCACAGT CAACATTAAG 
GGTCTGGAAC TACAGGAGAC ATCATGCCAC ACAGTGGAAG CTCGGCGTGT GGACGAGGTT 
TTCGAAGGAG CGTTTGATCA CGTGGAGCGG CCAGGCCAGT GTTTCAGACT ATTGAACCCT 
AACTGGGGTA ACGCTCTTAT CCCCTGCTCT CTGTTGCCCG TCAGGGTTTA CTCTGATGCC 
CAGAGCGTTC TAACGGGAAT TATTGACTCA CCCGATCACC TGCGTCAGCT AAACTCTGAC 
TTCCTCAAAA CACTTGTCTG GATTCTATTG CGTTACTGTG TACAGGAATT CACATGCGGT 
GCTCAGCGAA CTAAAGGTCA GACTTTACAG ACCTCTGGAC GTAAGTCACA AAGCTCCCAG 
CATCCTGTGC CCTCAGAGGC AGGTCCAGCT GTGATCAAAG TGGAGACTCA AGTACGCCCT 
GAATCCTCCT CGTCCTCTCA CTTGAGGGGT CTGGACAGCT CCAGCCTGTC GTCCTTTGCT 
GATTGGTCAG ACGAGGATGA TCTGTTTGGT CCAGTCCCTG TGCGACGACC GATGAGAATG 
ATGGTGCGGA CAGATGAAGC GCAGACAGAG TTAGGGCTGG GTGTGTCTCT TCCAGGTTCT 
GTCGAAATCC ACAGCTTGTA TGAGAGCATG GCTCTGTCTT CTCTACCGAC CCTTAGACCT 
CTGGGTCTGG GTTTGGGACT TGGCCTGCCC ATTATAGATA AAGGGAAAGA AAACATCCCT 
GCACCTACTT CGACAACTGT CCGGACCAAC TCCCAGCCTC TTCTCTTTAC CAGCCCTCAC 
TCAGAGCTCT TCTCTCTGCC CGCAGACTGG CGAACCGCCA ACTTGGCTTC CTCTAAACTC 
CAGGTTTTGC GACCCTGCTT CCCAGAGGCC TGGTTCTGCT TCTGTTTATC CCAGCTGGTG 
CTAGAGAGTT TGGGAGAAGG AGACTTTGAG CAGGTGACGC AGGGTTTACA GGAGGACCGT 
GCCTTGCAAG AGCTTTATAC CCAGGTGGTG CTATCATGCT GTGTGGCGCT AGGGGCAGAT 
GCTCAGGTCT TCAGCCCAAA TCTGCTGTTC AGGCTGTACT GTGGGGACGG CCCCTGGACT 
GAGGGGCTTG AGTGGCTCCG AGCCAATAAA GAGCTGCATC AGCTCACACT CAGAGCTTTC 
AGGTACAGCG TAAAACTATT GGTAGACCAG GCATCTCTTG GTCCAGTAGA GAGTCTGGAT 
GAGCTCTACA CTACTTTGCA GGATTACCAT AAAAACTGGT TCATCGGCCT GGTATCAGAT 
CGCAGTTGGC AAGATAGTGT TGTACAGGAG AAGCCCTTCC TGTTCTCTCT TGGACATGAC 
CTCACCATGG GGACGTACAC CAGCCGTGTG TTGTCCCTGC AGGAGAATCT GGTCCAAGTG 
GGAGCTCTAA ATGAGGAGGG GGTGAGGGGC CAGTGGGCCA ACCTGTCATG GGAACTATTG 
TATGCCACTA ATGACGATGA GGAGCGCTAC AGCATTCAGG CCCATCCAGT GATGCTGAGG 
AACCTCACTA TTCAAGCAGC AGATCCGCCT CTGGGATATC CCATATACTC ATCCCCTCCA 
ATTCACCTCC GCTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx4 ( XM_009307333.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305608.1 Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44128
Clone ID Related Accession (Same CDS sequence)	XM_009307333.3 , XM_009307333.2
Accession Version	XM_009307333.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pecanex-like protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTGT CATCAGTGTG TGTTGCAATC TGCACCTACT TCATTCCCAC TTCACTCGGT 
GTGGTTCTGT TCACCGTGGC CATGGGTTTC CTCTTCAGCC AGGACCTGAC CCAGCTGCTG 
GTGCTGGTAA AAGGGGTCTC TCCGACCCTG GGACTGGGCT GGTGGGGGCT TCCTCTTTCC 
CTGCTCTTCA TAATAGGAGC CTTGACCGAG GCTGGACTTC TTCATCAGCT GAACCTTGTC 
AACTCAGGGA ATCTAACATT GAATACGAAC TCTGTAGAGA ACTGGGAGTC TTCCGGTGTG 
GTTCCTCCAA ACCCACAGGA GGTGCTTGGC TGGCTTCTAA TTGCTCTGTT TTTCATCACT 
CGTCTGCTGA GAGAGCTTCA GGGGGCTTGC ATTATTGGAG GAGCCATGCT CAACCCACTC 
TACCCCAAAC GGGTCACAAC TGCACAGGCT TTCAGGAGGA AAACCCGTGG CCTGCGTGTT 
GCAGGAATCA TTCGGAGGAT CCTATTGAAC CTTGTAAGTC CGTTGACGAT GATTGCGTTC 
TTGTCCGTGG ATGAATCTTT AGGGAAGATA CACACAGCCT CTCTTGCCAT TGGCTTCACT 
CGGGCATTCA GGATGGTGTG GCAGAGCTCT GAGGCGGCTC TACTGCAGAT GGTGTTGGTG 
GTGATCATCA AACTTGCAGG GGGACACAGA GTTGCACACT GGCATGACTT GGGCACTACA 
GTACAGTTAA TACTGGTCTC TCTGGTTGTG AATCGTGTCT CACAGTTTGT ATGTAAGCTG 
AGTTTTGCTC TTACGGTGTT GATCACATCC TGGACTGAGA GTAAACAGCG TCGACAGTCA 
GCAGGAACTC TGCTGGCACT CAATGCTGCT TTGTGCCCAC TTCTACTGGC CGTCGTGGCT 
CTATCTGCTC TTCTCTCTGC CCCTCTCCTT CCACTATTCA CACTACCGGT GTTCCTGGTG 
GGATTTCCCA GGCCGCTGCG CTCATGGCCA GGCACTCCTG GCACCGCCTG CCCTTGCCCA 
GATTCTGTGT ATTACCAGCA GCTCTGCAAA AGGCTGGCAT CAGCTATAAG ACCTGCACTT 
GCTAATGGTG CATTTGGATC CTGCACTCCA GGGTCTCATT ACCTAGGCCG CTTTCAGGGT 
CGTCTTCTGT GGATCTCGAT TCTAGAAAAG GGCTATGGCT ATTGCACAGT CAACATTAAG 
GGTCTGGAAC TACAGGAGAC ATCATGCCAC ACAGTGGAAG CTCGGCGTGT GGACGAGGTT 
TTCGAAGGAG CGTTTGATCA CGTGGAGCGG CCAGGCCAGT GTTTCAGACT ATTGAACCCT 
AACTGGGGTA ACGCTCTTAT CCCCTGCTCT CTGTTGCCCG TCAGGGTTTA CTCTGATGCC 
CAGAGCGTTC TAACGGGAAT TATTGACTCA CCCGATCACC TGCGTCAGCT AAACTCTGAC 
TTCCTCAAAA CACTTGTCTG GATTCTATTG CGTTACTGTG TACAGGAATT CACATGCGGT 
GCTCAGCGAA CTAAAGGTCA GACTTTACAG ACCTCTGGAC GTAAGTCACA AAGCTCCCAG 
CATCCTGTGC CCTCAGAGGC AGGTCCAGCT GTGATCAAAG TGGAGACTCA AGTACGCCCT 
GAATCCTCCT CGTCCTCTCA CTTGAGGGGT CTGGACAGCT CCAGCCTGTC GTCCTTTGCT 
GATTGGTCAG ACGAGGATGA TCTGTTTGGT CCAGTCCCTG TGCGACGACC GATGAGAATG 
ATGGTGCGGA CAGATGAAGC GCAGACAGAG TTAGGGCTGG GTGTGTCTCT TCCAGGTTCT 
GTCGAAATCC ACAGCTTGTA TGAGAGCATG GCTCTGTCTT CTCTACCGAC CCTTAGACCT 
CTGGGTCTGG GTTTGGGACT TGGCCTGCCC ATTATAGATA AAGGGAAAGA AAACATCCCT 
GCACCTACTT CGACAACTGT CCGGACCAAC TCCCAGCCTC TTCTCTTTAC CAGCCCTCAC 
TCAGAGCTCT TCTCTCTGCC CGCAGACTGG CGAACCGCCA ACTTGGCTTC CTCTAAACTC 
CAGGTTTTGC GACCCTGCTT CCCAGAGGCC TGGTTCTGCT TCTGTTTATC CCAGCTGGTG 
CTAGAGAGTT TGGGAGAAGG AGACTTTGAG CAGGTGACGC AGGGTTTACA GGAGGACCGT 
GCCTTGCAAG AGCTTTATAC CCAGGTGGTG CTATCATGCT GTGTGGCGCT AGGGGCAGAT 
GCTCAGGTCT TCAGCCCAAA TCTGCTGTTC AGGCTGTACT GTGGGGACGG CCCCTGGACT 
GAGGGGCTTG AGTGGCTCCG AGCCAATAAA GAGCTGCATC AGCTCACACT CAGAGCTTTC 
AGGTACAGCG TAAAACTATT GGTAGACCAG GCATCTCTTG GTCCAGTAGA GAGTCTGGAT 
GAGCTCTACA CTACTTTGCA GGATTACCAT AAAAACTGGT TCATCGGCCT GGTATCAGAT 
CGCAGTTGGC AAGATAGTGT TGTACAGGAG AAGCCCTTCC TGTTCTCTCT TGGACATGAC 
CTCACCATGG GGACGTACAC CAGCCGTGTG TTGTCCCTGC AGGAGAATCT GGTCCAAGTG 
GGAGCTCTAA ATGAGGAGGG GGTGAGGGGC CAGTGGGCCA ACCTGTCATG GGAACTATTG 
TATGCCACTA ATGACGATGA GGAGCGCTAC AGCATTCAGG CCCATCCAGT GATGCTGAGG 
AACCTCACTA TTCAAGCAGC AGATCCGCCT CTGGGATATC CCATATACTC ATCCCCTCCA 
ATTCACCTCC GCTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305608.1
CDS	87..2867
Translation	MFLSSVCVAICTYFIPTSLGVVLFTVAMGFLFSQDLTQLLVLVKGVSPTLGLGWWGLPLSLLFIIGALTEAGLLHQLNLVNSGNLTLNTNSVENWESSGVVPPNPQEVLGWLLIALFFITRLLRELQGACIIGGAMLNPLYPKRVTTAQAFRRKTRGLRVAGIIRRILLNLVSPLTMIAFLSVDESLGKIHTASLAIGFTRAFRMVWQSSEAALLQMVLVVIIKLAGGHRVAHWHDLGTTVQLILVSLVVNRVSQFVCKLSFALTVLITSWTESKQRRQSAGTLLALNAALCPLLLAVVALSALLSAPLLPLFTLPVFLVGFPRPLRSWPGTPGTACPCPDSVYYQQLCKRLASAIRPALANGAFGSCTPGSHYLGRFQGRLLWISILEKGYGYCTVNIKGLELQETSCHTVEARRVDEVFEGAFDHVERPGQCFRLLNPNWGNALIPCSLLPVRVYSDAQSVLTGIIDSPDHLRQLNSDFLKTLVWILLRYCVQEFTCGAQRTKGQTLQTSGRKSQSSQHPVPSEAGPAVIKVETQVRPESSSSSHLRGLDSSSLSSFADWSDEDDLFGPVPVRRPMRMMVRTDEAQTELGLGVSLPGSVEIHSLYESMALSSLPTLRPLGLGLGLGLPIIDKGKENIPAPTSTTVRTNSQPLLFTSPHSELFSLPADWRTANLASSKLQVLRPCFPEAWFCFCLSQLVLESLGEGDFEQVTQGLQEDRALQELYTQVVLSCCVALGADAQVFSPNLLFRLYCGDGPWTEGLEWLRANKELHQLTLRAFRYSVKLLVDQASLGPVESLDELYTTLQDYHKNWFIGLVSDRSWQDSVVQEKPFLFSLGHDLTMGTYTSRVLSLQENLVQVGALNEEGVRGQWANLSWELLYATNDDEERYSIQAHPVMLRNLTIQAADPPLGYPIYSSPPIHLRCL

Target ORF information:

RefSeq Version	XM_009307333.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307333.2

ORF Insert Sequence:

ATGTTTTTGT CATCAGTGTG TGTTGCAATC TGCACCTACT TCATTCCCAC TTCACTCGGT 
GTGGTTCTGT TCACCGTGGC CATGGGTTTC CTCTTCAGCC AGGACCTGAC CCAGCTGCTG 
GTGCTGGTAA AAGGGGTCTC TCCGACCCTG GGACTGGGCT GGTGGGGGCT TCCTCTTTCC 
CTGCTCTTCA TAATAGGAGC CTTGACCGAG GCTGGACTTC TTCATCAGCT GAACCTTGTC 
AACTCAGGGA ATCTAACATT GAATACGAAC TCTGTAGAGA ACTGGGAGTC TTCCGGTGTG 
GTTCCTCCAA ACCCACAGGA GGTGCTTGGC TGGCTTCTAA TTGCTCTGTT TTTCATCACT 
CGTCTGCTGA GAGAGCTTCA GGGGGCTTGC ATTATTGGAG GAGCCATGCT CAACCCACTC 
TACCCCAAAC GGGTCACAAC TGCACAGGCT TTCAGGAGGA AAACCCGTGG CCTGCGTGTT 
GCAGGAATCA TTCGGAGGAT CCTATTGAAC CTTGTAAGTC CGTTGACGAT GATTGCGTTC 
TTGTCCGTGG ATGAATCTTT AGGGAAGATA CACACAGCCT CTCTTGCCAT TGGCTTCACT 
CGGGCATTCA GGATGGTGTG GCAGAGCTCT GAGGCGGCTC TACTGCAGAT GGTGTTGGTG 
GTGATCATCA AACTTGCAGG GGGACACAGA GTTGCACACT GGCATGACTT GGGCACTACA 
GTACAGTTAA TACTGGTCTC TCTGGTTGTG AATCGTGTCT CACAGTTTGT ATGTAAGCTG 
AGTTTTGCTC TTACGGTGTT GATCACATCC TGGACTGAGA GTAAACAGCG TCGACAGTCA 
GCAGGAACTC TGCTGGCACT CAATGCTGCT TTGTGCCCAC TTCTACTGGC CGTCGTGGCT 
CTATCTGCTC TTCTCTCTGC CCCTCTCCTT CCACTATTCA CACTACCGGT GTTCCTGGTG 
GGATTTCCCA GGCCGCTGCG CTCATGGCCA GGCACTCCTG GCACCGCCTG CCCTTGCCCA 
GATTCTGTGT ATTACCAGCA GCTCTGCAAA AGGCTGGCAT CAGCTATAAG ACCTGCACTT 
GCTAATGGTG CATTTGGATC CTGCACTCCA GGGTCTCATT ACCTAGGCCG CTTTCAGGGT 
CGTCTTCTGT GGATCTCGAT TCTAGAAAAG GGCTATGGCT ATTGCACAGT CAACATTAAG 
GGTCTGGAAC TACAGGAGAC ATCATGCCAC ACAGTGGAAG CTCGGCGTGT GGACGAGGTT 
TTCGAAGGAG CGTTTGATCA CGTGGAGCGG CCAGGCCAGT GTTTCAGACT ATTGAACCCT 
AACTGGGGTA ACGCTCTTAT CCCCTGCTCT CTGTTGCCCG TCAGGGTTTA CTCTGATGCC 
CAGAGCGTTC TAACGGGAAT TATTGACTCA CCCGATCACC TGCGTCAGCT AAACTCTGAC 
TTCCTCAAAA CACTTGTCTG GATTCTATTG CGTTACTGTG TACAGGAATT CACATGCGGT 
GCTCAGCGAA CTAAAGGTCA GACTTTACAG ACCTCTGGAC GTAAGTCACA AAGCTCCCAG 
CATCCTGTGC CCTCAGAGGC AGGTCCAGCT GTGATCAAAG TGGAGACTCA AGTACGCCCT 
GAATCCTCCT CGTCCTCTCA CTTGAGGGGT CTGGACAGCT CCAGCCTGTC GTCCTTTGCT 
GATTGGTCAG ACGAGGATGA TCTGTTTGGT CCAGTCCCTG TGCGACGACC GATGAGAATG 
ATGGTGCGGA CAGATGAAGC GCAGACAGAG TTAGGGCTGG GTGTGTCTCT TCCAGGTTCT 
GTCGAAATCC ACAGCTTGTA TGAGAGCATG GCTCTGTCTT CTCTACCGAC CCTTAGACCT 
CTGGGTCTGG GTTTGGGACT TGGCCTGCCC ATTATAGATA AAGGGAAAGA AAACATCCCT 
GCACCTACTT CGACAACTGT CCGGACCAAC TCCCAGCCTC TTCTCTTTAC CAGCCCTCAC 
TCAGAGCTCT TCTCTCTGCC CGCAGACTGG CGAACCGCCA ACTTGGCTTC CTCTAAACTC 
CAGGTTTTGC GACCCTGCTT CCCAGAGGCC TGGTTCTGCT TCTGTTTATC CCAGCTGGTG 
CTAGAGAGTT TGGGAGAAGG AGACTTTGAG CAGGTGACGC AGGGTTTACA GGAGGACCGT 
GCCTTGCAAG AGCTTTATAC CCAGGTGGTG CTATCATGCT GTGTGGCGCT AGGGGCAGAT 
GCTCAGGTCT TCAGCCCAAA TCTGCTGTTC AGGCTGTACT GTGGGGACGG CCCCTGGACT 
GAGGGGCTTG AGTGGCTCCG AGCCAATAAA GAGCTGCATC AGCTCACACT CAGAGCTTTC 
AGGTACAGCG TAAAACTATT GGTAGACCAG GCATCTCTTG GTCCAGTAGA GAGTCTGGAT 
GAGCTCTACA CTACTTTGCA GGATTACCAT AAAAACTGGT TCATCGGCCT GGTATCAGAT 
CGCAGTTGGC AAGATAGTGT TGTACAGGAG AAGCCCTTCC TGTTCTCTCT TGGACATGAC 
CTCACCATGG GGACGTACAC CAGCCGTGTG TTGTCCCTGC AGGAGAATCT GGTCCAAGTG 
GGAGCTCTAA ATGAGGAGGG GGTGAGGGGC CAGTGGGCCA ACCTGTCATG GGAACTATTG 
TATGCCACTA ATGACGATGA GGAGCGCTAC AGCATTCAGG CCCATCCAGT GATGCTGAGG 
AACCTCACTA TTCAAGCAGC AGATCCGCCT CTGGGATATC CCATATACTC ATCCCCTCCA 
ATTCACCTCC GCTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx4 ( NM_001081574.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001075043.1 Danio rerio pecanex 4 (pcnx4), mRNA.

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CloneID	ODa37983
Clone ID Related Accession (Same CDS sequence)	XM_009307332.3 , XM_009307332.2 , NM_001081574.1
Accession Version	NM_001081574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Danio rerio(zebrafish)
Product	pecanex-like protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133075.1. On Mar 11, 2007 this sequence version replaced XM_687006.2. ##Evidence-Data-START## Transcript exon combination :: BC133075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCCTG ATGTGCCCCT GCTCAATGAC TACAAACAGG AGTTCTTTTG GAAGCGCTTT 
CCTCAGACTG TCCTGGGGGG ACCTAGGCTG AAACTCGGCT ACTGTGCCCC GCCATATGTC 
TATGTGCACC AGCTGGTGTT ATTCCTGACA CCATGGCTAT TGGGAGGCAT CGGGACCCTC 
CTTTACCAGC TGAGGGTGCT GGACGAAGCA TTTGCGGGCA TTGTTTCCGG TGCGCTCATG 
CTGGGAGTTG GTGCCATCGT TCAGGGAATG GCACGGTGCA TGGCACGTAG GACCGGAGTT 
GTACAAAGAC TGCCTGCTGC CAATAACATC CTGGCAGACG AGGAAGAGGT GGAGTTTACT 
CACTGTGTGG CCCCTGAGAC AGTACGATTC GTGGTACCTG GGAAGAGATT TATGGGTAAT 
GTAGTTTTGC ACACGTGTCT CGCTGGAGCT CTCTGTGGGT TGTCAACATG GTACCTGCTG 
CCGCACAGAC TGAGCGGTTT GTATGGGCAC ACGGGTGCAG GATTGGGTGC CGTTGTGTCT 
TTGTTTGCTC TGAGCTGGGT GACGGTCTGT ATTGGGGAGT ATTCTCTCAT CGTGAACACG 
GCTACCGAAA CGGCCACATT TCAGCCACAG GACACTTATG AGATCACGCC GCTCAACAGG 
CCCATGTACA TACTTGCCTT CATCGCCGTG GATCTGGCTC TAAGGTTTTC AGATTCTGGT 
GTGGTGGAAC TGCAGGTTGC ATCTCAGGTT CTTCATGTGC TGTTTGTAGT TCTGCCAGTA 
CTCTGGGCTT TGGGAATGCT CTCTCCGCTG GACGCTCTTC TACTGTGGGC CATGGAGCAG 
ATCTTGGTGT ATGGTCTGGG TGGATCGGCC ATGTCCACCA ACATCAGGCT CTTAGTGATG 
TTTTTGTCAT CAGTGTGTGT TGCAATCTGC ACCTACTTCA TTCCCACTTC ACTCGGTGTG 
GTTCTGTTCA CCGTGGCCAT GGGTTTCCTC TTCAGCCAGG ACCTGACCCA GCTGCTGGTG 
CTGGTAAAAG GGGTCTCTCC GACCCTGGGA CTGGGCTGGT GGGGGCTTCC TCTTTCCCTG 
CTCTTCATAA TAGGAGCCTT GACCGAGGCT GGACTTCTTC ATCAGCTGAA CCTTGTCAAC 
TCAGGGAATC TAACATTGAA TACGAACTCT GTAGAGAACT GGGAGTCTTC CGGTGTGGTT 
CCTCCAAACC CACAGGAGGT GCTTGGCTGG CTTCTAATTG CTCTGTTTTT CATCACTCGT 
CTGCTGAGAG AGCTTCAGGG GGCTTGCATT ATTGGAGGAG CCATGCTCAA CCCACTCTAC 
CCCAAACGGG TCACAACTGC ACAGGCTTTC AGGAGGAAAA CCCGTGGCCT GCGTGTTGCA 
GGAATCATTC GGAGGATCCT ATTGAACCTT GTAAGTCCGT TGACGATGAT TGCGTTCTTG 
TCCGTGGATG AATCTTTAGG GAAGATACAC ACAGCCTCTC TTGCCATTGG CTTCACTCGG 
GCATTCAGGA TGGTGTGGCA GAGCTCTGAG GCGGCTCTAC TGCAGATGGT GTTGGTGGTG 
ATCATCAAAC TTGCAGGGGG ACACAGAGTT GCACACTGGC ATGACTTGGG CACTACAGTA 
CAGTTAATAC TGGTCTCTCT GGTTGTGAAT CGTGTCTCAC AGTTTGTATG TAAGCTGAGT 
TTTGCTCTTA CGGTGTTGAT CACATCCTGG ACTGAGAGTA AACAGCGTCG ACAGTCAGCA 
GGAACTCTGC TGGCACTCAA TGCTGCTTTG TGCCCACTTC TACTGGCCGT CGTGGCTCTA 
TCTGCTCTTC TCTCTGCCCC TCTCCTTCCA CTATTCACAC TACCGGTGTT CCTGGTGGGA 
TTTCCCAGGC CGCTGCGCTC ATGGCCAGGC ACTCCTGGCA CCGCCTGCCC TTGCCCAGAT 
TCTGTGTATT ACCAGCAGCT CTGCAAAAGG CTGGCATCAG CTATAAGACC TGCACTTGCT 
AATGGTGCAT TTGGATCCTG CACTCCAGGG TCTCATTACC TAGGCCGCTT TCAGGGTCGT 
CTTCTGTGGA TCTCGATTCT AGAAAAGGGC TATGGCTATT GCACAGTCAA CATTAAGGGT 
CTGGAACTAC AGGAGACATC ATGCCACACA GTGGAAGCTC GGCGTGTGGA CGAGGTTTTC 
GAAGGAGCGT TTGATCACGT GGAGCGGCCA GGCCAGTGTT TCAGACTATT GAACCCTAAC 
TGGGGTAACG CTCTTATCCC CTGCTCTCTG TTGCCCGTCA GGGTTTACTC TGATGCCCAG 
AGCGTTCTAA CGGGAATTAT TGACTCACCC GATCACCTGC GTCAGCTAAA CTCTGACTTC 
CTCAAAACAC TTGTCTGGAT TCTATTGCGT TACTGTGTAC AGGAATTCAC ATGCGGTGCT 
CAGCGAACTA AAGGTCAGAC TTTACAGACC TCTGGACGTA AGTCACAAAG CTCCCAGCAT 
CCTGTGCCCT CAGAGGCAGG TCCAGCTGTG ATCAAAGTGG AGACTCAAGT ACGCCCTGAA 
TCCTCCTCGT CCTCTCACTT GAGGGGTCTG GACAGCTCCA GCCTGTCGTC CTTTGCTGAT 
TGGTCAGACG AGGATGATCT GTTTGGTCCA GTCCCTGTGC GACGACCGAT GAGAATGATG 
GTGCGGACAG ATGAAGCGCA GACAGAGTTA GGGCTGGGTG TGTCTCTTCC AGGTTCTGTC 
GAAATCCACA GCTTGTATGA GAGCATGGCT CTGTCTTCTC TACCGACCCT TAGACCTCTG 
GGTCTGGGTT TGGGACTTGG CCTGCCCATT ATAGATAAAG GGAAAGAAAA CATCCCTGCA 
CCTACTTCGA CAACTGTCCG GACCAACTCC CAGCCTCTTC TCTTTACCAG CCCTCACTCA 
GAGCTCTTCT CTCTGCCCGC AGACTGGCGA ACCGCCAACT TGGCTTCCTC TAAACTCCAG 
GTTTTGCGAC CCTGCTTCCC AGAGGCCTGG TTCTGCTTCT GTTTATCCCA GCTGGTGCTA 
GAGAGTTTGG GAGAAGGAGA CTTTGAGCAG GTGACGCAGG GTTTACAGGA GGACCGTGCC 
TTGCAAGAGC TTTATACCCA GGTGGTGCTA TCATGCTGTG TGGCGCTAGG GGCAGATGCT 
CAGGTCTTCA GCCCAAATCT GCTGTTCAGG CTGTACTGTG GGGACGGCCC CTGGACTGAG 
GGGCTTGAGT GGCTCCGAGC CAATAAAGAG CTGCATCAGC TCACACTCAG AGCTTTCAGG 
TACAGCGTAA AACTATTGGT AGACCAGGCA TCTCTTGGTC CAGTAGAGAG TCTGGATGAG 
CTCTACACTA CTTTGCAGGA TTACCATAAA AACTGGTTCA TCGGCCTGGT ATCAGATCGC 
AGTTGGCAAG ATAGTGTTGT ACAGGAGAAG CCCTTCCTGT TCTCTCTTGG ACATGACCTC 
ACCATGGGGA CGTACACCAG CCGTGTGTTG TCCCTGCAGG AGAATCTGGT CCAAGTGGGA 
GCTCTAAATG AGGAGGGGGT GAGGGGCCAG TGGGCCAACC TGTCATGGGA ACTATTGTAT 
GCCACTAATG ACGATGAGGA GCGCTACAGC ATTCAGGCCC ATCCAGTGAT GCTGAGGAAC 
CTCACTATTC AAGCAGCAGA TCCGCCTCTG GGATATCCCA TATACTCATC CCCTCCAATT 
CACCTCCGCT GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075043.1
CDS	185..3862
Translation	MGPDVPLLNDYKQEFFWKRFPQTVLGGPRLKLGYCAPPYVYVHQLVLFLTPWLLGGIGTLLYQLRVLDEAFAGIVSGALMLGVGAIVQGMARCMARRTGVVQRLPAANNILADEEEVEFTHCVAPETVRFVVPGKRFMGNVVLHTCLAGALCGLSTWYLLPHRLSGLYGHTGAGLGAVVSLFALSWVTVCIGEYSLIVNTATETATFQPQDTYEITPLNRPMYILAFIAVDLALRFSDSGVVELQVASQVLHVLFVVLPVLWALGMLSPLDALLLWAMEQILVYGLGGSAMSTNIRLLVMFLSSVCVAICTYFIPTSLGVVLFTVAMGFLFSQDLTQLLVLVKGVSPTLGLGWWGLPLSLLFIIGALTEAGLLHQLNLVNSGNLTLNTNSVENWESSGVVPPNPQEVLGWLLIALFFITRLLRELQGACIIGGAMLNPLYPKRVTTAQAFRRKTRGLRVAGIIRRILLNLVSPLTMIAFLSVDESLGKIHTASLAIGFTRAFRMVWQSSEAALLQMVLVVIIKLAGGHRVAHWHDLGTTVQLILVSLVVNRVSQFVCKLSFALTVLITSWTESKQRRQSAGTLLALNAALCPLLLAVVALSALLSAPLLPLFTLPVFLVGFPRPLRSWPGTPGTACPCPDSVYYQQLCKRLASAIRPALANGAFGSCTPGSHYLGRFQGRLLWISILEKGYGYCTVNIKGLELQETSCHTVEARRVDEVFEGAFDHVERPGQCFRLLNPNWGNALIPCSLLPVRVYSDAQSVLTGIIDSPDHLRQLNSDFLKTLVWILLRYCVQEFTCGAQRTKGQTLQTSGRKSQSSQHPVPSEAGPAVIKVETQVRPESSSSSHLRGLDSSSLSSFADWSDEDDLFGPVPVRRPMRMMVRTDEAQTELGLGVSLPGSVEIHSLYESMALSSLPTLRPLGLGLGLGLPIIDKGKENIPAPTSTTVRTNSQPLLFTSPHSELFSLPADWRTANLASSKLQVLRPCFPEAWFCFCLSQLVLESLGEGDFEQVTQGLQEDRALQELYTQVVLSCCVALGADAQVFSPNLLFRLYCGDGPWTEGLEWLRANKELHQLTLRAFRYSVKLLVDQASLGPVESLDELYTTLQDYHKNWFIGLVSDRSWQDSVVQEKPFLFSLGHDLTMGTYTSRVLSLQENLVQVGALNEEGVRGQWANLSWELLYATNDDEERYSIQAHPVMLRNLTIQAADPPLGYPIYSSPPIHLRCL

Target ORF information:

RefSeq Version	NM_001081574.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pecanex 4 (pcnx4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081574.1

ORF Insert Sequence:

ATGGGGCCTG ATGTGCCCCT GCTCAATGAC TACAAACAGG AGTTCTTTTG GAAGCGCTTT 
CCTCAGACTG TCCTGGGGGG ACCTAGGCTG AAACTCGGCT ACTGTGCCCC GCCATATGTC 
TATGTGCACC AGCTGGTGTT ATTCCTGACA CCATGGCTAT TGGGAGGCAT CGGGACCCTC 
CTTTACCAGC TGAGGGTGCT GGACGAAGCA TTTGCGGGCA TTGTTTCCGG TGCGCTCATG 
CTGGGAGTTG GTGCCATCGT TCAGGGAATG GCACGGTGCA TGGCACGTAG GACCGGAGTT 
GTACAAAGAC TGCCTGCTGC CAATAACATC CTGGCAGACG AGGAAGAGGT GGAGTTTACT 
CACTGTGTGG CCCCTGAGAC AGTACGATTC GTGGTACCTG GGAAGAGATT TATGGGTAAT 
GTAGTTTTGC ACACGTGTCT CGCTGGAGCT CTCTGTGGGT TGTCAACATG GTACCTGCTG 
CCGCACAGAC TGAGCGGTTT GTATGGGCAC ACGGGTGCAG GATTGGGTGC CGTTGTGTCT 
TTGTTTGCTC TGAGCTGGGT GACGGTCTGT ATTGGGGAGT ATTCTCTCAT CGTGAACACG 
GCTACCGAAA CGGCCACATT TCAGCCACAG GACACTTATG AGATCACGCC GCTCAACAGG 
CCCATGTACA TACTTGCCTT CATCGCCGTG GATCTGGCTC TAAGGTTTTC AGATTCTGGT 
GTGGTGGAAC TGCAGGTTGC ATCTCAGGTT CTTCATGTGC TGTTTGTAGT TCTGCCAGTA 
CTCTGGGCTT TGGGAATGCT CTCTCCGCTG GACGCTCTTC TACTGTGGGC CATGGAGCAG 
ATCTTGGTGT ATGGTCTGGG TGGATCGGCC ATGTCCACCA ACATCAGGCT CTTAGTGATG 
TTTTTGTCAT CAGTGTGTGT TGCAATCTGC ACCTACTTCA TTCCCACTTC ACTCGGTGTG 
GTTCTGTTCA CCGTGGCCAT GGGTTTCCTC TTCAGCCAGG ACCTGACCCA GCTGCTGGTG 
CTGGTAAAAG GGGTCTCTCC GACCCTGGGA CTGGGCTGGT GGGGGCTTCC TCTTTCCCTG 
CTCTTCATAA TAGGAGCCTT GACCGAGGCT GGACTTCTTC ATCAGCTGAA CCTTGTCAAC 
TCAGGGAATC TAACATTGAA TACGAACTCT GTAGAGAACT GGGAGTCTTC CGGTGTGGTT 
CCTCCAAACC CACAGGAGGT GCTTGGCTGG CTTCTAATTG CTCTGTTTTT CATCACTCGT 
CTGCTGAGAG AGCTTCAGGG GGCTTGCATT ATTGGAGGAG CCATGCTCAA CCCACTCTAC 
CCCAAACGGG TCACAACTGC ACAGGCTTTC AGGAGGAAAA CCCGTGGCCT GCGTGTTGCA 
GGAATCATTC GGAGGATCCT ATTGAACCTT GTAAGTCCGT TGACGATGAT TGCGTTCTTG 
TCCGTGGATG AATCTTTAGG GAAGATACAC ACAGCCTCTC TTGCCATTGG CTTCACTCGG 
GCATTCAGGA TGGTGTGGCA GAGCTCTGAG GCGGCTCTAC TGCAGATGGT GTTGGTGGTG 
ATCATCAAAC TTGCAGGGGG ACACAGAGTT GCACACTGGC ATGACTTGGG CACTACAGTA 
CAGTTAATAC TGGTCTCTCT GGTTGTGAAT CGTGTCTCAC AGTTTGTATG TAAGCTGAGT 
TTTGCTCTTA CGGTGTTGAT CACATCCTGG ACTGAGAGTA AACAGCGTCG ACAGTCAGCA 
GGAACTCTGC TGGCACTCAA TGCTGCTTTG TGCCCACTTC TACTGGCCGT CGTGGCTCTA 
TCTGCTCTTC TCTCTGCCCC TCTCCTTCCA CTATTCACAC TACCGGTGTT CCTGGTGGGA 
TTTCCCAGGC CGCTGCGCTC ATGGCCAGGC ACTCCTGGCA CCGCCTGCCC TTGCCCAGAT 
TCTGTGTATT ACCAGCAGCT CTGCAAAAGG CTGGCATCAG CTATAAGACC TGCACTTGCT 
AATGGTGCAT TTGGATCCTG CACTCCAGGG TCTCATTACC TAGGCCGCTT TCAGGGTCGT 
CTTCTGTGGA TCTCGATTCT AGAAAAGGGC TATGGCTATT GCACAGTCAA CATTAAGGGT 
CTGGAACTAC AGGAGACATC ATGCCACACA GTGGAAGCTC GGCGTGTGGA CGAGGTTTTC 
GAAGGAGCGT TTGATCACGT GGAGCGGCCA GGCCAGTGTT TCAGACTATT GAACCCTAAC 
TGGGGTAACG CTCTTATCCC CTGCTCTCTG TTGCCCGTCA GGGTTTACTC TGATGCCCAG 
AGCGTTCTAA CGGGAATTAT TGACTCACCC GATCACCTGC GTCAGCTAAA CTCTGACTTC 
CTCAAAACAC TTGTCTGGAT TCTATTGCGT TACTGTGTAC AGGAATTCAC ATGCGGTGCT 
CAGCGAACTA AAGGTCAGAC TTTACAGACC TCTGGACGTA AGTCACAAAG CTCCCAGCAT 
CCTGTGCCCT CAGAGGCAGG TCCAGCTGTG ATCAAAGTGG AGACTCAAGT ACGCCCTGAA 
TCCTCCTCGT CCTCTCACTT GAGGGGTCTG GACAGCTCCA GCCTGTCGTC CTTTGCTGAT 
TGGTCAGACG AGGATGATCT GTTTGGTCCA GTCCCTGTGC GACGACCGAT GAGAATGATG 
GTGCGGACAG ATGAAGCGCA GACAGAGTTA GGGCTGGGTG TGTCTCTTCC AGGTTCTGTC 
GAAATCCACA GCTTGTATGA GAGCATGGCT CTGTCTTCTC TACCGACCCT TAGACCTCTG 
GGTCTGGGTT TGGGACTTGG CCTGCCCATT ATAGATAAAG GGAAAGAAAA CATCCCTGCA 
CCTACTTCGA CAACTGTCCG GACCAACTCC CAGCCTCTTC TCTTTACCAG CCCTCACTCA 
GAGCTCTTCT CTCTGCCCGC AGACTGGCGA ACCGCCAACT TGGCTTCCTC TAAACTCCAG 
GTTTTGCGAC CCTGCTTCCC AGAGGCCTGG TTCTGCTTCT GTTTATCCCA GCTGGTGCTA 
GAGAGTTTGG GAGAAGGAGA CTTTGAGCAG GTGACGCAGG GTTTACAGGA GGACCGTGCC 
TTGCAAGAGC TTTATACCCA GGTGGTGCTA TCATGCTGTG TGGCGCTAGG GGCAGATGCT 
CAGGTCTTCA GCCCAAATCT GCTGTTCAGG CTGTACTGTG GGGACGGCCC CTGGACTGAG 
GGGCTTGAGT GGCTCCGAGC CAATAAAGAG CTGCATCAGC TCACACTCAG AGCTTTCAGG 
TACAGCGTAA AACTATTGGT AGACCAGGCA TCTCTTGGTC CAGTAGAGAG TCTGGATGAG 
CTCTACACTA CTTTGCAGGA TTACCATAAA AACTGGTTCA TCGGCCTGGT ATCAGATCGC 
AGTTGGCAAG ATAGTGTTGT ACAGGAGAAG CCCTTCCTGT TCTCTCTTGG ACATGACCTC 
ACCATGGGGA CGTACACCAG CCGTGTGTTG TCCCTGCAGG AGAATCTGGT CCAAGTGGGA 
GCTCTAAATG AGGAGGGGGT GAGGGGCCAG TGGGCCAACC TGTCATGGGA ACTATTGTAT 
GCCACTAATG ACGATGAGGA GCGCTACAGC ATTCAGGCCC ATCCAGTGAT GCTGAGGAAC 
CTCACTATTC AAGCAGCAGA TCCGCCTCTG GGATATCCCA TATACTCATC CCCTCCAATT 
CACCTCCGCT GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx4 ( XM_009307332.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305607.1 Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X1, mRNA.

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CloneID	ODa37983
Clone ID Related Accession (Same CDS sequence)	XM_009307332.3 , XM_009307332.2 , NM_001081574.1
Accession Version	XM_009307332.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pecanex-like protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307332.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCTG ATGTGCCCCT GCTCAATGAC TACAAACAGG AGTTCTTTTG GAAGCGCTTT 
CCTCAGACTG TCCTGGGGGG ACCTAGGCTG AAACTCGGCT ACTGTGCCCC GCCATATGTC 
TATGTGCACC AGCTGGTGTT ATTCCTGACA CCATGGCTAT TGGGAGGCAT CGGGACCCTC 
CTTTACCAGC TGAGGGTGCT GGACGAAGCA TTTGCGGGCA TTGTTTCCGG TGCGCTCATG 
CTGGGAGTTG GTGCCATCGT TCAGGGAATG GCACGGTGCA TGGCACGTAG GACCGGAGTT 
GTACAAAGAC TGCCTGCTGC CAATAACATC CTGGCAGACG AGGAAGAGGT GGAGTTTACT 
CACTGTGTGG CCCCTGAGAC AGTACGATTC GTGGTACCTG GGAAGAGATT TATGGGTAAT 
GTAGTTTTGC ACACGTGTCT CGCTGGAGCT CTCTGTGGGT TGTCAACATG GTACCTGCTG 
CCGCACAGAC TGAGCGGTTT GTATGGGCAC ACGGGTGCAG GATTGGGTGC CGTTGTGTCT 
TTGTTTGCTC TGAGCTGGGT GACGGTCTGT ATTGGGGAGT ATTCTCTCAT CGTGAACACG 
GCTACCGAAA CGGCCACATT TCAGCCACAG GACACTTATG AGATCACGCC GCTCAACAGG 
CCCATGTACA TACTTGCCTT CATCGCCGTG GATCTGGCTC TAAGGTTTTC AGATTCTGGT 
GTGGTGGAAC TGCAGGTTGC ATCTCAGGTT CTTCATGTGC TGTTTGTAGT TCTGCCAGTA 
CTCTGGGCTT TGGGAATGCT CTCTCCGCTG GACGCTCTTC TACTGTGGGC CATGGAGCAG 
ATCTTGGTGT ATGGTCTGGG TGGATCGGCC ATGTCCACCA ACATCAGGCT CTTAGTGATG 
TTTTTGTCAT CAGTGTGTGT TGCAATCTGC ACCTACTTCA TTCCCACTTC ACTCGGTGTG 
GTTCTGTTCA CCGTGGCCAT GGGTTTCCTC TTCAGCCAGG ACCTGACCCA GCTGCTGGTG 
CTGGTAAAAG GGGTCTCTCC GACCCTGGGA CTGGGCTGGT GGGGGCTTCC TCTTTCCCTG 
CTCTTCATAA TAGGAGCCTT GACCGAGGCT GGACTTCTTC ATCAGCTGAA CCTTGTCAAC 
TCAGGGAATC TAACATTGAA TACGAACTCT GTAGAGAACT GGGAGTCTTC CGGTGTGGTT 
CCTCCAAACC CACAGGAGGT GCTTGGCTGG CTTCTAATTG CTCTGTTTTT CATCACTCGT 
CTGCTGAGAG AGCTTCAGGG GGCTTGCATT ATTGGAGGAG CCATGCTCAA CCCACTCTAC 
CCCAAACGGG TCACAACTGC ACAGGCTTTC AGGAGGAAAA CCCGTGGCCT GCGTGTTGCA 
GGAATCATTC GGAGGATCCT ATTGAACCTT GTAAGTCCGT TGACGATGAT TGCGTTCTTG 
TCCGTGGATG AATCTTTAGG GAAGATACAC ACAGCCTCTC TTGCCATTGG CTTCACTCGG 
GCATTCAGGA TGGTGTGGCA GAGCTCTGAG GCGGCTCTAC TGCAGATGGT GTTGGTGGTG 
ATCATCAAAC TTGCAGGGGG ACACAGAGTT GCACACTGGC ATGACTTGGG CACTACAGTA 
CAGTTAATAC TGGTCTCTCT GGTTGTGAAT CGTGTCTCAC AGTTTGTATG TAAGCTGAGT 
TTTGCTCTTA CGGTGTTGAT CACATCCTGG ACTGAGAGTA AACAGCGTCG ACAGTCAGCA 
GGAACTCTGC TGGCACTCAA TGCTGCTTTG TGCCCACTTC TACTGGCCGT CGTGGCTCTA 
TCTGCTCTTC TCTCTGCCCC TCTCCTTCCA CTATTCACAC TACCGGTGTT CCTGGTGGGA 
TTTCCCAGGC CGCTGCGCTC ATGGCCAGGC ACTCCTGGCA CCGCCTGCCC TTGCCCAGAT 
TCTGTGTATT ACCAGCAGCT CTGCAAAAGG CTGGCATCAG CTATAAGACC TGCACTTGCT 
AATGGTGCAT TTGGATCCTG CACTCCAGGG TCTCATTACC TAGGCCGCTT TCAGGGTCGT 
CTTCTGTGGA TCTCGATTCT AGAAAAGGGC TATGGCTATT GCACAGTCAA CATTAAGGGT 
CTGGAACTAC AGGAGACATC ATGCCACACA GTGGAAGCTC GGCGTGTGGA CGAGGTTTTC 
GAAGGAGCGT TTGATCACGT GGAGCGGCCA GGCCAGTGTT TCAGACTATT GAACCCTAAC 
TGGGGTAACG CTCTTATCCC CTGCTCTCTG TTGCCCGTCA GGGTTTACTC TGATGCCCAG 
AGCGTTCTAA CGGGAATTAT TGACTCACCC GATCACCTGC GTCAGCTAAA CTCTGACTTC 
CTCAAAACAC TTGTCTGGAT TCTATTGCGT TACTGTGTAC AGGAATTCAC ATGCGGTGCT 
CAGCGAACTA AAGGTCAGAC TTTACAGACC TCTGGACGTA AGTCACAAAG CTCCCAGCAT 
CCTGTGCCCT CAGAGGCAGG TCCAGCTGTG ATCAAAGTGG AGACTCAAGT ACGCCCTGAA 
TCCTCCTCGT CCTCTCACTT GAGGGGTCTG GACAGCTCCA GCCTGTCGTC CTTTGCTGAT 
TGGTCAGACG AGGATGATCT GTTTGGTCCA GTCCCTGTGC GACGACCGAT GAGAATGATG 
GTGCGGACAG ATGAAGCGCA GACAGAGTTA GGGCTGGGTG TGTCTCTTCC AGGTTCTGTC 
GAAATCCACA GCTTGTATGA GAGCATGGCT CTGTCTTCTC TACCGACCCT TAGACCTCTG 
GGTCTGGGTT TGGGACTTGG CCTGCCCATT ATAGATAAAG GGAAAGAAAA CATCCCTGCA 
CCTACTTCGA CAACTGTCCG GACCAACTCC CAGCCTCTTC TCTTTACCAG CCCTCACTCA 
GAGCTCTTCT CTCTGCCCGC AGACTGGCGA ACCGCCAACT TGGCTTCCTC TAAACTCCAG 
GTTTTGCGAC CCTGCTTCCC AGAGGCCTGG TTCTGCTTCT GTTTATCCCA GCTGGTGCTA 
GAGAGTTTGG GAGAAGGAGA CTTTGAGCAG GTGACGCAGG GTTTACAGGA GGACCGTGCC 
TTGCAAGAGC TTTATACCCA GGTGGTGCTA TCATGCTGTG TGGCGCTAGG GGCAGATGCT 
CAGGTCTTCA GCCCAAATCT GCTGTTCAGG CTGTACTGTG GGGACGGCCC CTGGACTGAG 
GGGCTTGAGT GGCTCCGAGC CAATAAAGAG CTGCATCAGC TCACACTCAG AGCTTTCAGG 
TACAGCGTAA AACTATTGGT AGACCAGGCA TCTCTTGGTC CAGTAGAGAG TCTGGATGAG 
CTCTACACTA CTTTGCAGGA TTACCATAAA AACTGGTTCA TCGGCCTGGT ATCAGATCGC 
AGTTGGCAAG ATAGTGTTGT ACAGGAGAAG CCCTTCCTGT TCTCTCTTGG ACATGACCTC 
ACCATGGGGA CGTACACCAG CCGTGTGTTG TCCCTGCAGG AGAATCTGGT CCAAGTGGGA 
GCTCTAAATG AGGAGGGGGT GAGGGGCCAG TGGGCCAACC TGTCATGGGA ACTATTGTAT 
GCCACTAATG ACGATGAGGA GCGCTACAGC ATTCAGGCCC ATCCAGTGAT GCTGAGGAAC 
CTCACTATTC AAGCAGCAGA TCCGCCTCTG GGATATCCCA TATACTCATC CCCTCCAATT 
CACCTCCGCT GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305607.1
CDS	306..3983
Translation	MGPDVPLLNDYKQEFFWKRFPQTVLGGPRLKLGYCAPPYVYVHQLVLFLTPWLLGGIGTLLYQLRVLDEAFAGIVSGALMLGVGAIVQGMARCMARRTGVVQRLPAANNILADEEEVEFTHCVAPETVRFVVPGKRFMGNVVLHTCLAGALCGLSTWYLLPHRLSGLYGHTGAGLGAVVSLFALSWVTVCIGEYSLIVNTATETATFQPQDTYEITPLNRPMYILAFIAVDLALRFSDSGVVELQVASQVLHVLFVVLPVLWALGMLSPLDALLLWAMEQILVYGLGGSAMSTNIRLLVMFLSSVCVAICTYFIPTSLGVVLFTVAMGFLFSQDLTQLLVLVKGVSPTLGLGWWGLPLSLLFIIGALTEAGLLHQLNLVNSGNLTLNTNSVENWESSGVVPPNPQEVLGWLLIALFFITRLLRELQGACIIGGAMLNPLYPKRVTTAQAFRRKTRGLRVAGIIRRILLNLVSPLTMIAFLSVDESLGKIHTASLAIGFTRAFRMVWQSSEAALLQMVLVVIIKLAGGHRVAHWHDLGTTVQLILVSLVVNRVSQFVCKLSFALTVLITSWTESKQRRQSAGTLLALNAALCPLLLAVVALSALLSAPLLPLFTLPVFLVGFPRPLRSWPGTPGTACPCPDSVYYQQLCKRLASAIRPALANGAFGSCTPGSHYLGRFQGRLLWISILEKGYGYCTVNIKGLELQETSCHTVEARRVDEVFEGAFDHVERPGQCFRLLNPNWGNALIPCSLLPVRVYSDAQSVLTGIIDSPDHLRQLNSDFLKTLVWILLRYCVQEFTCGAQRTKGQTLQTSGRKSQSSQHPVPSEAGPAVIKVETQVRPESSSSSHLRGLDSSSLSSFADWSDEDDLFGPVPVRRPMRMMVRTDEAQTELGLGVSLPGSVEIHSLYESMALSSLPTLRPLGLGLGLGLPIIDKGKENIPAPTSTTVRTNSQPLLFTSPHSELFSLPADWRTANLASSKLQVLRPCFPEAWFCFCLSQLVLESLGEGDFEQVTQGLQEDRALQELYTQVVLSCCVALGADAQVFSPNLLFRLYCGDGPWTEGLEWLRANKELHQLTLRAFRYSVKLLVDQASLGPVESLDELYTTLQDYHKNWFIGLVSDRSWQDSVVQEKPFLFSLGHDLTMGTYTSRVLSLQENLVQVGALNEEGVRGQWANLSWELLYATNDDEERYSIQAHPVMLRNLTIQAADPPLGYPIYSSPPIHLRCL

Target ORF information:

RefSeq Version	XM_009307332.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307332.3

ORF Insert Sequence:

ATGGGGCCTG ATGTGCCCCT GCTCAATGAC TACAAACAGG AGTTCTTTTG GAAGCGCTTT 
CCTCAGACTG TCCTGGGGGG ACCTAGGCTG AAACTCGGCT ACTGTGCCCC GCCATATGTC 
TATGTGCACC AGCTGGTGTT ATTCCTGACA CCATGGCTAT TGGGAGGCAT CGGGACCCTC 
CTTTACCAGC TGAGGGTGCT GGACGAAGCA TTTGCGGGCA TTGTTTCCGG TGCGCTCATG 
CTGGGAGTTG GTGCCATCGT TCAGGGAATG GCACGGTGCA TGGCACGTAG GACCGGAGTT 
GTACAAAGAC TGCCTGCTGC CAATAACATC CTGGCAGACG AGGAAGAGGT GGAGTTTACT 
CACTGTGTGG CCCCTGAGAC AGTACGATTC GTGGTACCTG GGAAGAGATT TATGGGTAAT 
GTAGTTTTGC ACACGTGTCT CGCTGGAGCT CTCTGTGGGT TGTCAACATG GTACCTGCTG 
CCGCACAGAC TGAGCGGTTT GTATGGGCAC ACGGGTGCAG GATTGGGTGC CGTTGTGTCT 
TTGTTTGCTC TGAGCTGGGT GACGGTCTGT ATTGGGGAGT ATTCTCTCAT CGTGAACACG 
GCTACCGAAA CGGCCACATT TCAGCCACAG GACACTTATG AGATCACGCC GCTCAACAGG 
CCCATGTACA TACTTGCCTT CATCGCCGTG GATCTGGCTC TAAGGTTTTC AGATTCTGGT 
GTGGTGGAAC TGCAGGTTGC ATCTCAGGTT CTTCATGTGC TGTTTGTAGT TCTGCCAGTA 
CTCTGGGCTT TGGGAATGCT CTCTCCGCTG GACGCTCTTC TACTGTGGGC CATGGAGCAG 
ATCTTGGTGT ATGGTCTGGG TGGATCGGCC ATGTCCACCA ACATCAGGCT CTTAGTGATG 
TTTTTGTCAT CAGTGTGTGT TGCAATCTGC ACCTACTTCA TTCCCACTTC ACTCGGTGTG 
GTTCTGTTCA CCGTGGCCAT GGGTTTCCTC TTCAGCCAGG ACCTGACCCA GCTGCTGGTG 
CTGGTAAAAG GGGTCTCTCC GACCCTGGGA CTGGGCTGGT GGGGGCTTCC TCTTTCCCTG 
CTCTTCATAA TAGGAGCCTT GACCGAGGCT GGACTTCTTC ATCAGCTGAA CCTTGTCAAC 
TCAGGGAATC TAACATTGAA TACGAACTCT GTAGAGAACT GGGAGTCTTC CGGTGTGGTT 
CCTCCAAACC CACAGGAGGT GCTTGGCTGG CTTCTAATTG CTCTGTTTTT CATCACTCGT 
CTGCTGAGAG AGCTTCAGGG GGCTTGCATT ATTGGAGGAG CCATGCTCAA CCCACTCTAC 
CCCAAACGGG TCACAACTGC ACAGGCTTTC AGGAGGAAAA CCCGTGGCCT GCGTGTTGCA 
GGAATCATTC GGAGGATCCT ATTGAACCTT GTAAGTCCGT TGACGATGAT TGCGTTCTTG 
TCCGTGGATG AATCTTTAGG GAAGATACAC ACAGCCTCTC TTGCCATTGG CTTCACTCGG 
GCATTCAGGA TGGTGTGGCA GAGCTCTGAG GCGGCTCTAC TGCAGATGGT GTTGGTGGTG 
ATCATCAAAC TTGCAGGGGG ACACAGAGTT GCACACTGGC ATGACTTGGG CACTACAGTA 
CAGTTAATAC TGGTCTCTCT GGTTGTGAAT CGTGTCTCAC AGTTTGTATG TAAGCTGAGT 
TTTGCTCTTA CGGTGTTGAT CACATCCTGG ACTGAGAGTA AACAGCGTCG ACAGTCAGCA 
GGAACTCTGC TGGCACTCAA TGCTGCTTTG TGCCCACTTC TACTGGCCGT CGTGGCTCTA 
TCTGCTCTTC TCTCTGCCCC TCTCCTTCCA CTATTCACAC TACCGGTGTT CCTGGTGGGA 
TTTCCCAGGC CGCTGCGCTC ATGGCCAGGC ACTCCTGGCA CCGCCTGCCC TTGCCCAGAT 
TCTGTGTATT ACCAGCAGCT CTGCAAAAGG CTGGCATCAG CTATAAGACC TGCACTTGCT 
AATGGTGCAT TTGGATCCTG CACTCCAGGG TCTCATTACC TAGGCCGCTT TCAGGGTCGT 
CTTCTGTGGA TCTCGATTCT AGAAAAGGGC TATGGCTATT GCACAGTCAA CATTAAGGGT 
CTGGAACTAC AGGAGACATC ATGCCACACA GTGGAAGCTC GGCGTGTGGA CGAGGTTTTC 
GAAGGAGCGT TTGATCACGT GGAGCGGCCA GGCCAGTGTT TCAGACTATT GAACCCTAAC 
TGGGGTAACG CTCTTATCCC CTGCTCTCTG TTGCCCGTCA GGGTTTACTC TGATGCCCAG 
AGCGTTCTAA CGGGAATTAT TGACTCACCC GATCACCTGC GTCAGCTAAA CTCTGACTTC 
CTCAAAACAC TTGTCTGGAT TCTATTGCGT TACTGTGTAC AGGAATTCAC ATGCGGTGCT 
CAGCGAACTA AAGGTCAGAC TTTACAGACC TCTGGACGTA AGTCACAAAG CTCCCAGCAT 
CCTGTGCCCT CAGAGGCAGG TCCAGCTGTG ATCAAAGTGG AGACTCAAGT ACGCCCTGAA 
TCCTCCTCGT CCTCTCACTT GAGGGGTCTG GACAGCTCCA GCCTGTCGTC CTTTGCTGAT 
TGGTCAGACG AGGATGATCT GTTTGGTCCA GTCCCTGTGC GACGACCGAT GAGAATGATG 
GTGCGGACAG ATGAAGCGCA GACAGAGTTA GGGCTGGGTG TGTCTCTTCC AGGTTCTGTC 
GAAATCCACA GCTTGTATGA GAGCATGGCT CTGTCTTCTC TACCGACCCT TAGACCTCTG 
GGTCTGGGTT TGGGACTTGG CCTGCCCATT ATAGATAAAG GGAAAGAAAA CATCCCTGCA 
CCTACTTCGA CAACTGTCCG GACCAACTCC CAGCCTCTTC TCTTTACCAG CCCTCACTCA 
GAGCTCTTCT CTCTGCCCGC AGACTGGCGA ACCGCCAACT TGGCTTCCTC TAAACTCCAG 
GTTTTGCGAC CCTGCTTCCC AGAGGCCTGG TTCTGCTTCT GTTTATCCCA GCTGGTGCTA 
GAGAGTTTGG GAGAAGGAGA CTTTGAGCAG GTGACGCAGG GTTTACAGGA GGACCGTGCC 
TTGCAAGAGC TTTATACCCA GGTGGTGCTA TCATGCTGTG TGGCGCTAGG GGCAGATGCT 
CAGGTCTTCA GCCCAAATCT GCTGTTCAGG CTGTACTGTG GGGACGGCCC CTGGACTGAG 
GGGCTTGAGT GGCTCCGAGC CAATAAAGAG CTGCATCAGC TCACACTCAG AGCTTTCAGG 
TACAGCGTAA AACTATTGGT AGACCAGGCA TCTCTTGGTC CAGTAGAGAG TCTGGATGAG 
CTCTACACTA CTTTGCAGGA TTACCATAAA AACTGGTTCA TCGGCCTGGT ATCAGATCGC 
AGTTGGCAAG ATAGTGTTGT ACAGGAGAAG CCCTTCCTGT TCTCTCTTGG ACATGACCTC 
ACCATGGGGA CGTACACCAG CCGTGTGTTG TCCCTGCAGG AGAATCTGGT CCAAGTGGGA 
GCTCTAAATG AGGAGGGGGT GAGGGGCCAG TGGGCCAACC TGTCATGGGA ACTATTGTAT 
GCCACTAATG ACGATGAGGA GCGCTACAGC ATTCAGGCCC ATCCAGTGAT GCTGAGGAAC 
CTCACTATTC AAGCAGCAGA TCCGCCTCTG GGATATCCCA TATACTCATC CCCTCCAATT 
CACCTCCGCT GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx4 ( XM_009307332.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305607.1 Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X1, mRNA.

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CloneID	ODa37983
Clone ID Related Accession (Same CDS sequence)	XM_009307332.3 , XM_009307332.2 , NM_001081574.1
Accession Version	XM_009307332.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pecanex-like protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307332.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCTG ATGTGCCCCT GCTCAATGAC TACAAACAGG AGTTCTTTTG GAAGCGCTTT 
CCTCAGACTG TCCTGGGGGG ACCTAGGCTG AAACTCGGCT ACTGTGCCCC GCCATATGTC 
TATGTGCACC AGCTGGTGTT ATTCCTGACA CCATGGCTAT TGGGAGGCAT CGGGACCCTC 
CTTTACCAGC TGAGGGTGCT GGACGAAGCA TTTGCGGGCA TTGTTTCCGG TGCGCTCATG 
CTGGGAGTTG GTGCCATCGT TCAGGGAATG GCACGGTGCA TGGCACGTAG GACCGGAGTT 
GTACAAAGAC TGCCTGCTGC CAATAACATC CTGGCAGACG AGGAAGAGGT GGAGTTTACT 
CACTGTGTGG CCCCTGAGAC AGTACGATTC GTGGTACCTG GGAAGAGATT TATGGGTAAT 
GTAGTTTTGC ACACGTGTCT CGCTGGAGCT CTCTGTGGGT TGTCAACATG GTACCTGCTG 
CCGCACAGAC TGAGCGGTTT GTATGGGCAC ACGGGTGCAG GATTGGGTGC CGTTGTGTCT 
TTGTTTGCTC TGAGCTGGGT GACGGTCTGT ATTGGGGAGT ATTCTCTCAT CGTGAACACG 
GCTACCGAAA CGGCCACATT TCAGCCACAG GACACTTATG AGATCACGCC GCTCAACAGG 
CCCATGTACA TACTTGCCTT CATCGCCGTG GATCTGGCTC TAAGGTTTTC AGATTCTGGT 
GTGGTGGAAC TGCAGGTTGC ATCTCAGGTT CTTCATGTGC TGTTTGTAGT TCTGCCAGTA 
CTCTGGGCTT TGGGAATGCT CTCTCCGCTG GACGCTCTTC TACTGTGGGC CATGGAGCAG 
ATCTTGGTGT ATGGTCTGGG TGGATCGGCC ATGTCCACCA ACATCAGGCT CTTAGTGATG 
TTTTTGTCAT CAGTGTGTGT TGCAATCTGC ACCTACTTCA TTCCCACTTC ACTCGGTGTG 
GTTCTGTTCA CCGTGGCCAT GGGTTTCCTC TTCAGCCAGG ACCTGACCCA GCTGCTGGTG 
CTGGTAAAAG GGGTCTCTCC GACCCTGGGA CTGGGCTGGT GGGGGCTTCC TCTTTCCCTG 
CTCTTCATAA TAGGAGCCTT GACCGAGGCT GGACTTCTTC ATCAGCTGAA CCTTGTCAAC 
TCAGGGAATC TAACATTGAA TACGAACTCT GTAGAGAACT GGGAGTCTTC CGGTGTGGTT 
CCTCCAAACC CACAGGAGGT GCTTGGCTGG CTTCTAATTG CTCTGTTTTT CATCACTCGT 
CTGCTGAGAG AGCTTCAGGG GGCTTGCATT ATTGGAGGAG CCATGCTCAA CCCACTCTAC 
CCCAAACGGG TCACAACTGC ACAGGCTTTC AGGAGGAAAA CCCGTGGCCT GCGTGTTGCA 
GGAATCATTC GGAGGATCCT ATTGAACCTT GTAAGTCCGT TGACGATGAT TGCGTTCTTG 
TCCGTGGATG AATCTTTAGG GAAGATACAC ACAGCCTCTC TTGCCATTGG CTTCACTCGG 
GCATTCAGGA TGGTGTGGCA GAGCTCTGAG GCGGCTCTAC TGCAGATGGT GTTGGTGGTG 
ATCATCAAAC TTGCAGGGGG ACACAGAGTT GCACACTGGC ATGACTTGGG CACTACAGTA 
CAGTTAATAC TGGTCTCTCT GGTTGTGAAT CGTGTCTCAC AGTTTGTATG TAAGCTGAGT 
TTTGCTCTTA CGGTGTTGAT CACATCCTGG ACTGAGAGTA AACAGCGTCG ACAGTCAGCA 
GGAACTCTGC TGGCACTCAA TGCTGCTTTG TGCCCACTTC TACTGGCCGT CGTGGCTCTA 
TCTGCTCTTC TCTCTGCCCC TCTCCTTCCA CTATTCACAC TACCGGTGTT CCTGGTGGGA 
TTTCCCAGGC CGCTGCGCTC ATGGCCAGGC ACTCCTGGCA CCGCCTGCCC TTGCCCAGAT 
TCTGTGTATT ACCAGCAGCT CTGCAAAAGG CTGGCATCAG CTATAAGACC TGCACTTGCT 
AATGGTGCAT TTGGATCCTG CACTCCAGGG TCTCATTACC TAGGCCGCTT TCAGGGTCGT 
CTTCTGTGGA TCTCGATTCT AGAAAAGGGC TATGGCTATT GCACAGTCAA CATTAAGGGT 
CTGGAACTAC AGGAGACATC ATGCCACACA GTGGAAGCTC GGCGTGTGGA CGAGGTTTTC 
GAAGGAGCGT TTGATCACGT GGAGCGGCCA GGCCAGTGTT TCAGACTATT GAACCCTAAC 
TGGGGTAACG CTCTTATCCC CTGCTCTCTG TTGCCCGTCA GGGTTTACTC TGATGCCCAG 
AGCGTTCTAA CGGGAATTAT TGACTCACCC GATCACCTGC GTCAGCTAAA CTCTGACTTC 
CTCAAAACAC TTGTCTGGAT TCTATTGCGT TACTGTGTAC AGGAATTCAC ATGCGGTGCT 
CAGCGAACTA AAGGTCAGAC TTTACAGACC TCTGGACGTA AGTCACAAAG CTCCCAGCAT 
CCTGTGCCCT CAGAGGCAGG TCCAGCTGTG ATCAAAGTGG AGACTCAAGT ACGCCCTGAA 
TCCTCCTCGT CCTCTCACTT GAGGGGTCTG GACAGCTCCA GCCTGTCGTC CTTTGCTGAT 
TGGTCAGACG AGGATGATCT GTTTGGTCCA GTCCCTGTGC GACGACCGAT GAGAATGATG 
GTGCGGACAG ATGAAGCGCA GACAGAGTTA GGGCTGGGTG TGTCTCTTCC AGGTTCTGTC 
GAAATCCACA GCTTGTATGA GAGCATGGCT CTGTCTTCTC TACCGACCCT TAGACCTCTG 
GGTCTGGGTT TGGGACTTGG CCTGCCCATT ATAGATAAAG GGAAAGAAAA CATCCCTGCA 
CCTACTTCGA CAACTGTCCG GACCAACTCC CAGCCTCTTC TCTTTACCAG CCCTCACTCA 
GAGCTCTTCT CTCTGCCCGC AGACTGGCGA ACCGCCAACT TGGCTTCCTC TAAACTCCAG 
GTTTTGCGAC CCTGCTTCCC AGAGGCCTGG TTCTGCTTCT GTTTATCCCA GCTGGTGCTA 
GAGAGTTTGG GAGAAGGAGA CTTTGAGCAG GTGACGCAGG GTTTACAGGA GGACCGTGCC 
TTGCAAGAGC TTTATACCCA GGTGGTGCTA TCATGCTGTG TGGCGCTAGG GGCAGATGCT 
CAGGTCTTCA GCCCAAATCT GCTGTTCAGG CTGTACTGTG GGGACGGCCC CTGGACTGAG 
GGGCTTGAGT GGCTCCGAGC CAATAAAGAG CTGCATCAGC TCACACTCAG AGCTTTCAGG 
TACAGCGTAA AACTATTGGT AGACCAGGCA TCTCTTGGTC CAGTAGAGAG TCTGGATGAG 
CTCTACACTA CTTTGCAGGA TTACCATAAA AACTGGTTCA TCGGCCTGGT ATCAGATCGC 
AGTTGGCAAG ATAGTGTTGT ACAGGAGAAG CCCTTCCTGT TCTCTCTTGG ACATGACCTC 
ACCATGGGGA CGTACACCAG CCGTGTGTTG TCCCTGCAGG AGAATCTGGT CCAAGTGGGA 
GCTCTAAATG AGGAGGGGGT GAGGGGCCAG TGGGCCAACC TGTCATGGGA ACTATTGTAT 
GCCACTAATG ACGATGAGGA GCGCTACAGC ATTCAGGCCC ATCCAGTGAT GCTGAGGAAC 
CTCACTATTC AAGCAGCAGA TCCGCCTCTG GGATATCCCA TATACTCATC CCCTCCAATT 
CACCTCCGCT GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305607.1
CDS	305..3982
Translation	MGPDVPLLNDYKQEFFWKRFPQTVLGGPRLKLGYCAPPYVYVHQLVLFLTPWLLGGIGTLLYQLRVLDEAFAGIVSGALMLGVGAIVQGMARCMARRTGVVQRLPAANNILADEEEVEFTHCVAPETVRFVVPGKRFMGNVVLHTCLAGALCGLSTWYLLPHRLSGLYGHTGAGLGAVVSLFALSWVTVCIGEYSLIVNTATETATFQPQDTYEITPLNRPMYILAFIAVDLALRFSDSGVVELQVASQVLHVLFVVLPVLWALGMLSPLDALLLWAMEQILVYGLGGSAMSTNIRLLVMFLSSVCVAICTYFIPTSLGVVLFTVAMGFLFSQDLTQLLVLVKGVSPTLGLGWWGLPLSLLFIIGALTEAGLLHQLNLVNSGNLTLNTNSVENWESSGVVPPNPQEVLGWLLIALFFITRLLRELQGACIIGGAMLNPLYPKRVTTAQAFRRKTRGLRVAGIIRRILLNLVSPLTMIAFLSVDESLGKIHTASLAIGFTRAFRMVWQSSEAALLQMVLVVIIKLAGGHRVAHWHDLGTTVQLILVSLVVNRVSQFVCKLSFALTVLITSWTESKQRRQSAGTLLALNAALCPLLLAVVALSALLSAPLLPLFTLPVFLVGFPRPLRSWPGTPGTACPCPDSVYYQQLCKRLASAIRPALANGAFGSCTPGSHYLGRFQGRLLWISILEKGYGYCTVNIKGLELQETSCHTVEARRVDEVFEGAFDHVERPGQCFRLLNPNWGNALIPCSLLPVRVYSDAQSVLTGIIDSPDHLRQLNSDFLKTLVWILLRYCVQEFTCGAQRTKGQTLQTSGRKSQSSQHPVPSEAGPAVIKVETQVRPESSSSSHLRGLDSSSLSSFADWSDEDDLFGPVPVRRPMRMMVRTDEAQTELGLGVSLPGSVEIHSLYESMALSSLPTLRPLGLGLGLGLPIIDKGKENIPAPTSTTVRTNSQPLLFTSPHSELFSLPADWRTANLASSKLQVLRPCFPEAWFCFCLSQLVLESLGEGDFEQVTQGLQEDRALQELYTQVVLSCCVALGADAQVFSPNLLFRLYCGDGPWTEGLEWLRANKELHQLTLRAFRYSVKLLVDQASLGPVESLDELYTTLQDYHKNWFIGLVSDRSWQDSVVQEKPFLFSLGHDLTMGTYTSRVLSLQENLVQVGALNEEGVRGQWANLSWELLYATNDDEERYSIQAHPVMLRNLTIQAADPPLGYPIYSSPPIHLRCL

Target ORF information:

RefSeq Version	XM_009307332.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pecanex-like 4 (Drosophila) (pcnxl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307332.2

ORF Insert Sequence:

ATGGGGCCTG ATGTGCCCCT GCTCAATGAC TACAAACAGG AGTTCTTTTG GAAGCGCTTT 
CCTCAGACTG TCCTGGGGGG ACCTAGGCTG AAACTCGGCT ACTGTGCCCC GCCATATGTC 
TATGTGCACC AGCTGGTGTT ATTCCTGACA CCATGGCTAT TGGGAGGCAT CGGGACCCTC 
CTTTACCAGC TGAGGGTGCT GGACGAAGCA TTTGCGGGCA TTGTTTCCGG TGCGCTCATG 
CTGGGAGTTG GTGCCATCGT TCAGGGAATG GCACGGTGCA TGGCACGTAG GACCGGAGTT 
GTACAAAGAC TGCCTGCTGC CAATAACATC CTGGCAGACG AGGAAGAGGT GGAGTTTACT 
CACTGTGTGG CCCCTGAGAC AGTACGATTC GTGGTACCTG GGAAGAGATT TATGGGTAAT 
GTAGTTTTGC ACACGTGTCT CGCTGGAGCT CTCTGTGGGT TGTCAACATG GTACCTGCTG 
CCGCACAGAC TGAGCGGTTT GTATGGGCAC ACGGGTGCAG GATTGGGTGC CGTTGTGTCT 
TTGTTTGCTC TGAGCTGGGT GACGGTCTGT ATTGGGGAGT ATTCTCTCAT CGTGAACACG 
GCTACCGAAA CGGCCACATT TCAGCCACAG GACACTTATG AGATCACGCC GCTCAACAGG 
CCCATGTACA TACTTGCCTT CATCGCCGTG GATCTGGCTC TAAGGTTTTC AGATTCTGGT 
GTGGTGGAAC TGCAGGTTGC ATCTCAGGTT CTTCATGTGC TGTTTGTAGT TCTGCCAGTA 
CTCTGGGCTT TGGGAATGCT CTCTCCGCTG GACGCTCTTC TACTGTGGGC CATGGAGCAG 
ATCTTGGTGT ATGGTCTGGG TGGATCGGCC ATGTCCACCA ACATCAGGCT CTTAGTGATG 
TTTTTGTCAT CAGTGTGTGT TGCAATCTGC ACCTACTTCA TTCCCACTTC ACTCGGTGTG 
GTTCTGTTCA CCGTGGCCAT GGGTTTCCTC TTCAGCCAGG ACCTGACCCA GCTGCTGGTG 
CTGGTAAAAG GGGTCTCTCC GACCCTGGGA CTGGGCTGGT GGGGGCTTCC TCTTTCCCTG 
CTCTTCATAA TAGGAGCCTT GACCGAGGCT GGACTTCTTC ATCAGCTGAA CCTTGTCAAC 
TCAGGGAATC TAACATTGAA TACGAACTCT GTAGAGAACT GGGAGTCTTC CGGTGTGGTT 
CCTCCAAACC CACAGGAGGT GCTTGGCTGG CTTCTAATTG CTCTGTTTTT CATCACTCGT 
CTGCTGAGAG AGCTTCAGGG GGCTTGCATT ATTGGAGGAG CCATGCTCAA CCCACTCTAC 
CCCAAACGGG TCACAACTGC ACAGGCTTTC AGGAGGAAAA CCCGTGGCCT GCGTGTTGCA 
GGAATCATTC GGAGGATCCT ATTGAACCTT GTAAGTCCGT TGACGATGAT TGCGTTCTTG 
TCCGTGGATG AATCTTTAGG GAAGATACAC ACAGCCTCTC TTGCCATTGG CTTCACTCGG 
GCATTCAGGA TGGTGTGGCA GAGCTCTGAG GCGGCTCTAC TGCAGATGGT GTTGGTGGTG 
ATCATCAAAC TTGCAGGGGG ACACAGAGTT GCACACTGGC ATGACTTGGG CACTACAGTA 
CAGTTAATAC TGGTCTCTCT GGTTGTGAAT CGTGTCTCAC AGTTTGTATG TAAGCTGAGT 
TTTGCTCTTA CGGTGTTGAT CACATCCTGG ACTGAGAGTA AACAGCGTCG ACAGTCAGCA 
GGAACTCTGC TGGCACTCAA TGCTGCTTTG TGCCCACTTC TACTGGCCGT CGTGGCTCTA 
TCTGCTCTTC TCTCTGCCCC TCTCCTTCCA CTATTCACAC TACCGGTGTT CCTGGTGGGA 
TTTCCCAGGC CGCTGCGCTC ATGGCCAGGC ACTCCTGGCA CCGCCTGCCC TTGCCCAGAT 
TCTGTGTATT ACCAGCAGCT CTGCAAAAGG CTGGCATCAG CTATAAGACC TGCACTTGCT 
AATGGTGCAT TTGGATCCTG CACTCCAGGG TCTCATTACC TAGGCCGCTT TCAGGGTCGT 
CTTCTGTGGA TCTCGATTCT AGAAAAGGGC TATGGCTATT GCACAGTCAA CATTAAGGGT 
CTGGAACTAC AGGAGACATC ATGCCACACA GTGGAAGCTC GGCGTGTGGA CGAGGTTTTC 
GAAGGAGCGT TTGATCACGT GGAGCGGCCA GGCCAGTGTT TCAGACTATT GAACCCTAAC 
TGGGGTAACG CTCTTATCCC CTGCTCTCTG TTGCCCGTCA GGGTTTACTC TGATGCCCAG 
AGCGTTCTAA CGGGAATTAT TGACTCACCC GATCACCTGC GTCAGCTAAA CTCTGACTTC 
CTCAAAACAC TTGTCTGGAT TCTATTGCGT TACTGTGTAC AGGAATTCAC ATGCGGTGCT 
CAGCGAACTA AAGGTCAGAC TTTACAGACC TCTGGACGTA AGTCACAAAG CTCCCAGCAT 
CCTGTGCCCT CAGAGGCAGG TCCAGCTGTG ATCAAAGTGG AGACTCAAGT ACGCCCTGAA 
TCCTCCTCGT CCTCTCACTT GAGGGGTCTG GACAGCTCCA GCCTGTCGTC CTTTGCTGAT 
TGGTCAGACG AGGATGATCT GTTTGGTCCA GTCCCTGTGC GACGACCGAT GAGAATGATG 
GTGCGGACAG ATGAAGCGCA GACAGAGTTA GGGCTGGGTG TGTCTCTTCC AGGTTCTGTC 
GAAATCCACA GCTTGTATGA GAGCATGGCT CTGTCTTCTC TACCGACCCT TAGACCTCTG 
GGTCTGGGTT TGGGACTTGG CCTGCCCATT ATAGATAAAG GGAAAGAAAA CATCCCTGCA 
CCTACTTCGA CAACTGTCCG GACCAACTCC CAGCCTCTTC TCTTTACCAG CCCTCACTCA 
GAGCTCTTCT CTCTGCCCGC AGACTGGCGA ACCGCCAACT TGGCTTCCTC TAAACTCCAG 
GTTTTGCGAC CCTGCTTCCC AGAGGCCTGG TTCTGCTTCT GTTTATCCCA GCTGGTGCTA 
GAGAGTTTGG GAGAAGGAGA CTTTGAGCAG GTGACGCAGG GTTTACAGGA GGACCGTGCC 
TTGCAAGAGC TTTATACCCA GGTGGTGCTA TCATGCTGTG TGGCGCTAGG GGCAGATGCT 
CAGGTCTTCA GCCCAAATCT GCTGTTCAGG CTGTACTGTG GGGACGGCCC CTGGACTGAG 
GGGCTTGAGT GGCTCCGAGC CAATAAAGAG CTGCATCAGC TCACACTCAG AGCTTTCAGG 
TACAGCGTAA AACTATTGGT AGACCAGGCA TCTCTTGGTC CAGTAGAGAG TCTGGATGAG 
CTCTACACTA CTTTGCAGGA TTACCATAAA AACTGGTTCA TCGGCCTGGT ATCAGATCGC 
AGTTGGCAAG ATAGTGTTGT ACAGGAGAAG CCCTTCCTGT TCTCTCTTGG ACATGACCTC 
ACCATGGGGA CGTACACCAG CCGTGTGTTG TCCCTGCAGG AGAATCTGGT CCAAGTGGGA 
GCTCTAAATG AGGAGGGGGT GAGGGGCCAG TGGGCCAACC TGTCATGGGA ACTATTGTAT 
GCCACTAATG ACGATGAGGA GCGCTACAGC ATTCAGGCCC ATCCAGTGAT GCTGAGGAAC 
CTCACTATTC AAGCAGCAGA TCCGCCTCTG GGATATCCCA TATACTCATC CCCTCCAATT 
CACCTCCGCT GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Genetic association analyses highlight biological pathways underlying mitral valve prolapse.
Nature genetics47(10)1206-11(2015 Oct)
Dina C,Bouatia-Naji N,Tucker N,Delling FN,Toomer K,Durst R,Perrocheau M,Fernandez-Friera L,Solis J,,Le Tourneau T,Chen MH,Probst V,Bosse Y,Pibarot P,Zelenika D,Lathrop M,Hercberg S,Roussel R,Benjamin EJ,Bonnet F,Lo SH,Dolmatova E,Simonet F,Lecointe S,Kyndt F,Redon R,Le Marec H,Froguel P,Ellinor PT,Vasan RS,Bruneval P,Markwald RR,Norris RA,Milan DJ,Slaugenhaupt SA,Levine RA,Schott JJ,Hagege AA,,Jeunemaitre X,

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pcnx4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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