kdm6bb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kdm6bb
Entrez Gene ID	563644
Full Name	lysine (K)-specific demethylase 6B, b
Synonyms	jmjd3,kdm6b,si:ch211-237l4.7,zC237L4.7
General protein information	Preferred Names lysine (K)-specific demethylase 6B, b Names lysine (K)-specific demethylase 6B, b jumonji domain containing 3, histone lysine demethylase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030178.2	NP_001025349.2	lysine (K)-specific demethylase 6B, b
XM_005172623.3	XP_005172680.1	lysine (K)-specific demethylase 6B, b isoform X1
XM_005172621.3	XP_005172678.1	lysine (K)-specific demethylase 6B, b isoform X1
XM_005172621.4	XP_005172678.1	lysine (K)-specific demethylase 6B, b isoform X1
XM_021479186.1	XP_021334861.1	lysine (K)-specific demethylase 6B, b isoform X1
XM_005172623.4	XP_005172680.1	lysine (K)-specific demethylase 6B, b isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	kdm6bb	NP_001025349.1
Danio rerio (zebrafish)	kdm6ba	XP_005172298.1

Gene Ontology
Bibliography

Related articles in PubMed

Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation.
Akerberg AA, Henner A, Stewart S, Stankunas K
Developmental biology426(1)84-96(2017 06)

Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
Brocal I, White RJ, Dooley CM, Carruthers SN, Clark R, Hall A, Busch-Nentwich EM, Stemple DL, Kettleborough RN
BMC genomics17259(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

loss of both kdm6ba and kdm6bb shows Kdm6b proteins share redundant and pleiotropic roles in organogenesis without impacting initial cell fate specification.
Title: Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation.

Data demonstrate that histone modifications silence promoters of numerous genes involved in zebrafish caudal fin regeneration, and that Kdm6b.1 may be the histone demethylase required during regeneration.
Title: A histone demethylase is necessary for regeneration in zebrafish.

The following kdm6bb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm6bb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa16069	NM_001030178.2 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa16069	XM_005172621.4 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa16069	XM_005172623.4 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa16069	XM_021479186.1 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa16069	XM_005172621.3	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa16069	XM_005172623.3	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kdm6bb ( NM_001030178.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025349.2 Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), mRNA.

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CloneID	ODa16069
Clone ID Related Accession (Same CDS sequence)	NM_001030178.2 , XM_005172623.3 , XM_005172621.3 , XM_005172621.4 , XM_021479186.1 , XM_005172623.4
Accession Version	NM_001030178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	lysine (K)-specific demethylase 6B, b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL807389.7. On or before Jun 16, 2016 this sequence version replaced XM_005172620.2, NM_001030178.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01008623.1, GFIL01013919.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025349.2
CDS	274..5433
Translation	MYHIAEQYSGRNSWDSFPSSGPNRAPWAPGNNRHWAPPSSGGNYQSPSQNYMAGRSYPNKAYNNRPPGFSRDRLHAHPRDRGNGKEQRVPRSCGPTQNQHHNPERSTLNYSSGYEGHLSSSNNHGNQPHRYGGPHQQQRSTGRPPQTQGEKWTHSAQSRSFQGPSLKRPVPPHNLSQPLREPSPSRDDCPSKRSRSINSHQNFHPRDKYFINRPPPSHPPRPWSPAYKSSMSWSLSEKRSSPSRFQESSHSVTRDHTYRPYSHSSPPSSVPNQGLHNRKLTKQGPAHHPVHDHPDSPPRGAKGGWDCPSPANLTVPYSQQHQLPPPQRHTGPSSSPATSVPQPRSNTAQHGWKTQNRSGKHSNSEMGRTTYELDSHQSVGGDRKRSSGSKHISRQRSPIQPYNSAEGRVRDCTEWRNPETGLKKHDQKDSDSPSEPTTQTSERGQRAQRSEMKKSRHSKHQNKLKSTHKSALSKLDSELLKHIQAKKRHKERLRRKQKKEEGELTEDTKERIEKKIEERKKKKTREGRKGHDKKTIHKSGRKLGQVKKRAKEERKQGDRKSETSISKTASPNLGTLYPSDSDPLLDDLLADPLHLSYIEEENEFSQHTSGFILPCKSPVNNNHCSPCQSTLEINTKVLEVDEKSDSEPDHVDTSDDDSVGTHDVSGSNPSLLNTHVSEEEPIRCKEVVPGLHTAPDLLPAHCTYSEELLRLDPPTSPPVLSWQGSPVLDLGDDDEDVKEEDMIGVLRRPVLQPSPTHSSPLREAIEISTGVDYCHSDLAKLYGLPESSKAMDTEEEDEEKTDGEQKDGSPDASGSSETNKPHLHQMDTFKSPSSAMASHRYTYRGGPFGRPPPSALVGVKYSSSLSLGPEIHPPDQQSPPASSPTTEIPDQVIHSLIQPTDKDKESETEEEIMKTGAKEDKIEVKGEETKVQKMDEQMEVKLAEPTKDPASTSLEEKESVMSPATVQAKLVHSCELLLNQNSSPVSKVMKVSGSKSDQRENEGDRQKDQLKTPRKAIRTMKEEEPETCVGRSIKAKHHKKGDRGENKKDIMKDKAETNSSSLSSEDAQKQLVEIRTKQVVPENQQIEIKVGKTESHKIKREKETDVKKDAIKEDAIKADSSATGCTSCTTNLQSSSTMTLFTRPPMLERVNLRNQHELSKIPLKELKICLVKIESRGRQTFRASEIEEKSIPLDAINIRNNAAEVIRACKGADIKNKFRESYLLPSLSVKPDLSCRTPIPRDKLNPPTPSIYLESKRDAFSPVLLQFCTDSKNAVTVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWNHSGSAQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKDSEDEDEEDKSKEKNEAKSNSQNNIGTTSTTTSSKQKAVGKIIKFGTNIDLSDPKRWKAQLQELLKLPAFMRVSSTANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWKAISDFCEKHGVDYLTGSWWPVLEDLYRSNIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLNAYQYQLALERFEWNEVKKVKSIVPMIHVSWNVARTIKITDPNTFKMIKHCLMQSIKHIQILRDQLVSAGKKISYQSRVKDEPAYYCNECDLEVFDLLFVTSENSSRKTYVVHCEDCARERSPGLNGVVVLEQYRIDELMNMYDNFTLSAVPCCR

Target ORF information:

RefSeq Version	NM_001030178.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030178.2

ORF Insert Sequence:

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6bb ( XM_005172623.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172680.1 Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X2, mRNA.

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CloneID	ODa16069
Clone ID Related Accession (Same CDS sequence)	NM_001030178.2 , XM_005172623.3 , XM_005172621.3 , XM_005172621.4 , XM_021479186.1 , XM_005172623.4
Accession Version	XM_005172623.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine (K)-specific demethylase 6B, b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172623.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172680.1
CDS	286..5445
Translation	MYHIAEQYSGRNSWDSFPSSGPNRAPWAPGNNRHWAPPSSGGNYQSPSQNYMAGRSYPNKAYNNRPPGFSRDRLHAHPRDRGNGKEQRVPRSCGPTQNQHHNPERSTLNYSSGYEGHLSSSNNHGNQPHRYGGPHQQQRSTGRPPQTQGEKWTHSAQSRSFQGPSLKRPVPPHNLSQPLREPSPSRDDCPSKRSRSINSHQNFHPRDKYFINRPPPSHPPRPWSPAYKSSMSWSLSEKRSSPSRFQESSHSVTRDHTYRPYSHSSPPSSVPNQGLHNRKLTKQGPAHHPVHDHPDSPPRGAKGGWDCPSPANLTVPYSQQHQLPPPQRHTGPSSSPATSVPQPRSNTAQHGWKTQNRSGKHSNSEMGRTTYELDSHQSVGGDRKRSSGSKHISRQRSPIQPYNSAEGRVRDCTEWRNPETGLKKHDQKDSDSPSEPTTQTSERGQRAQRSEMKKSRHSKHQNKLKSTHKSALSKLDSELLKHIQAKKRHKERLRRKQKKEEGELTEDTKERIEKKIEERKKKKTREGRKGHDKKTIHKSGRKLGQVKKRAKEERKQGDRKSETSISKTASPNLGTLYPSDSDPLLDDLLADPLHLSYIEEENEFSQHTSGFILPCKSPVNNNHCSPCQSTLEINTKVLEVDEKSDSEPDHVDTSDDDSVGTHDVSGSNPSLLNTHVSEEEPIRCKEVVPGLHTAPDLLPAHCTYSEELLRLDPPTSPPVLSWQGSPVLDLGDDDEDVKEEDMIGVLRRPVLQPSPTHSSPLREAIEISTGVDYCHSDLAKLYGLPESSKAMDTEEEDEEKTDGEQKDGSPDASGSSETNKPHLHQMDTFKSPSSAMASHRYTYRGGPFGRPPPSALVGVKYSSSLSLGPEIHPPDQQSPPASSPTTEIPDQVIHSLIQPTDKDKESETEEEIMKTGAKEDKIEVKGEETKVQKMDEQMEVKLAEPTKDPASTSLEEKESVMSPATVQAKLVHSCELLLNQNSSPVSKVMKVSGSKSDQRENEGDRQKDQLKTPRKAIRTMKEEEPETCVGRSIKAKHHKKGDRGENKKDIMKDKAETNSSSLSSEDAQKQLVEIRTKQVVPENQQIEIKVGKTESHKIKREKETDVKKDAIKEDAIKADSSATGCTSCTTNLQSSSTMTLFTRPPMLERVNLRNQHELSKIPLKELKICLVKIESRGRQTFRASEIEEKSIPLDAINIRNNAAEVIRACKGADIKNKFRESYLLPSLSVKPDLSCRTPIPRDKLNPPTPSIYLESKRDAFSPVLLQFCTDSKNAVTVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWNHSGSAQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKDSEDEDEEDKSKEKNEAKSNSQNNIGTTSTTTSSKQKAVGKIIKFGTNIDLSDPKRWKAQLQELLKLPAFMRVSSTANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWKAISDFCEKHGVDYLTGSWWPVLEDLYRSNIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLNAYQYQLALERFEWNEVKKVKSIVPMIHVSWNVARTIKITDPNTFKMIKHCLMQSIKHIQILRDQLVSAGKKISYQSRVKDEPAYYCNECDLEVFDLLFVTSENSSRKTYVVHCEDCARERSPGLNGVVVLEQYRIDELMNMYDNFTLSAVPCCR

Target ORF information:

RefSeq Version	XM_005172623.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172623.3

ORF Insert Sequence:

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6bb ( XM_005172621.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172678.1 Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16069
Clone ID Related Accession (Same CDS sequence)	NM_001030178.2 , XM_005172623.3 , XM_005172621.3 , XM_005172621.4 , XM_021479186.1 , XM_005172623.4
Accession Version	XM_005172621.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine (K)-specific demethylase 6B, b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172621.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172678.1
CDS	290..5449
Translation	MYHIAEQYSGRNSWDSFPSSGPNRAPWAPGNNRHWAPPSSGGNYQSPSQNYMAGRSYPNKAYNNRPPGFSRDRLHAHPRDRGNGKEQRVPRSCGPTQNQHHNPERSTLNYSSGYEGHLSSSNNHGNQPHRYGGPHQQQRSTGRPPQTQGEKWTHSAQSRSFQGPSLKRPVPPHNLSQPLREPSPSRDDCPSKRSRSINSHQNFHPRDKYFINRPPPSHPPRPWSPAYKSSMSWSLSEKRSSPSRFQESSHSVTRDHTYRPYSHSSPPSSVPNQGLHNRKLTKQGPAHHPVHDHPDSPPRGAKGGWDCPSPANLTVPYSQQHQLPPPQRHTGPSSSPATSVPQPRSNTAQHGWKTQNRSGKHSNSEMGRTTYELDSHQSVGGDRKRSSGSKHISRQRSPIQPYNSAEGRVRDCTEWRNPETGLKKHDQKDSDSPSEPTTQTSERGQRAQRSEMKKSRHSKHQNKLKSTHKSALSKLDSELLKHIQAKKRHKERLRRKQKKEEGELTEDTKERIEKKIEERKKKKTREGRKGHDKKTIHKSGRKLGQVKKRAKEERKQGDRKSETSISKTASPNLGTLYPSDSDPLLDDLLADPLHLSYIEEENEFSQHTSGFILPCKSPVNNNHCSPCQSTLEINTKVLEVDEKSDSEPDHVDTSDDDSVGTHDVSGSNPSLLNTHVSEEEPIRCKEVVPGLHTAPDLLPAHCTYSEELLRLDPPTSPPVLSWQGSPVLDLGDDDEDVKEEDMIGVLRRPVLQPSPTHSSPLREAIEISTGVDYCHSDLAKLYGLPESSKAMDTEEEDEEKTDGEQKDGSPDASGSSETNKPHLHQMDTFKSPSSAMASHRYTYRGGPFGRPPPSALVGVKYSSSLSLGPEIHPPDQQSPPASSPTTEIPDQVIHSLIQPTDKDKESETEEEIMKTGAKEDKIEVKGEETKVQKMDEQMEVKLAEPTKDPASTSLEEKESVMSPATVQAKLVHSCELLLNQNSSPVSKVMKVSGSKSDQRENEGDRQKDQLKTPRKAIRTMKEEEPETCVGRSIKAKHHKKGDRGENKKDIMKDKAETNSSSLSSEDAQKQLVEIRTKQVVPENQQIEIKVGKTESHKIKREKETDVKKDAIKEDAIKADSSATGCTSCTTNLQSSSTMTLFTRPPMLERVNLRNQHELSKIPLKELKICLVKIESRGRQTFRASEIEEKSIPLDAINIRNNAAEVIRACKGADIKNKFRESYLLPSLSVKPDLSCRTPIPRDKLNPPTPSIYLESKRDAFSPVLLQFCTDSKNAVTVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWNHSGSAQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKDSEDEDEEDKSKEKNEAKSNSQNNIGTTSTTTSSKQKAVGKIIKFGTNIDLSDPKRWKAQLQELLKLPAFMRVSSTANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWKAISDFCEKHGVDYLTGSWWPVLEDLYRSNIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLNAYQYQLALERFEWNEVKKVKSIVPMIHVSWNVARTIKITDPNTFKMIKHCLMQSIKHIQILRDQLVSAGKKISYQSRVKDEPAYYCNECDLEVFDLLFVTSENSSRKTYVVHCEDCARERSPGLNGVVVLEQYRIDELMNMYDNFTLSAVPCCR

Target ORF information:

RefSeq Version	XM_005172621.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172621.3

ORF Insert Sequence:

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6bb ( XM_005172621.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172678.1 Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X1, mRNA.

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CloneID	ODa16069
Clone ID Related Accession (Same CDS sequence)	NM_001030178.2 , XM_005172623.3 , XM_005172621.3 , XM_005172621.4 , XM_021479186.1 , XM_005172623.4
Accession Version	XM_005172621.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine (K)-specific demethylase 6B, b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172621.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172678.1
CDS	329..5488
Translation	MYHIAEQYSGRNSWDSFPSSGPNRAPWAPGNNRHWAPPSSGGNYQSPSQNYMAGRSYPNKAYNNRPPGFSRDRLHAHPRDRGNGKEQRVPRSCGPTQNQHHNPERSTLNYSSGYEGHLSSSNNHGNQPHRYGGPHQQQRSTGRPPQTQGEKWTHSAQSRSFQGPSLKRPVPPHNLSQPLREPSPSRDDCPSKRSRSINSHQNFHPRDKYFINRPPPSHPPRPWSPAYKSSMSWSLSEKRSSPSRFQESSHSVTRDHTYRPYSHSSPPSSVPNQGLHNRKLTKQGPAHHPVHDHPDSPPRGAKGGWDCPSPANLTVPYSQQHQLPPPQRHTGPSSSPATSVPQPRSNTAQHGWKTQNRSGKHSNSEMGRTTYELDSHQSVGGDRKRSSGSKHISRQRSPIQPYNSAEGRVRDCTEWRNPETGLKKHDQKDSDSPSEPTTQTSERGQRAQRSEMKKSRHSKHQNKLKSTHKSALSKLDSELLKHIQAKKRHKERLRRKQKKEEGELTEDTKERIEKKIEERKKKKTREGRKGHDKKTIHKSGRKLGQVKKRAKEERKQGDRKSETSISKTASPNLGTLYPSDSDPLLDDLLADPLHLSYIEEENEFSQHTSGFILPCKSPVNNNHCSPCQSTLEINTKVLEVDEKSDSEPDHVDTSDDDSVGTHDVSGSNPSLLNTHVSEEEPIRCKEVVPGLHTAPDLLPAHCTYSEELLRLDPPTSPPVLSWQGSPVLDLGDDDEDVKEEDMIGVLRRPVLQPSPTHSSPLREAIEISTGVDYCHSDLAKLYGLPESSKAMDTEEEDEEKTDGEQKDGSPDASGSSETNKPHLHQMDTFKSPSSAMASHRYTYRGGPFGRPPPSALVGVKYSSSLSLGPEIHPPDQQSPPASSPTTEIPDQVIHSLIQPTDKDKESETEEEIMKTGAKEDKIEVKGEETKVQKMDEQMEVKLAEPTKDPASTSLEEKESVMSPATVQAKLVHSCELLLNQNSSPVSKVMKVSGSKSDQRENEGDRQKDQLKTPRKAIRTMKEEEPETCVGRSIKAKHHKKGDRGENKKDIMKDKAETNSSSLSSEDAQKQLVEIRTKQVVPENQQIEIKVGKTESHKIKREKETDVKKDAIKEDAIKADSSATGCTSCTTNLQSSSTMTLFTRPPMLERVNLRNQHELSKIPLKELKICLVKIESRGRQTFRASEIEEKSIPLDAINIRNNAAEVIRACKGADIKNKFRESYLLPSLSVKPDLSCRTPIPRDKLNPPTPSIYLESKRDAFSPVLLQFCTDSKNAVTVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWNHSGSAQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKDSEDEDEEDKSKEKNEAKSNSQNNIGTTSTTTSSKQKAVGKIIKFGTNIDLSDPKRWKAQLQELLKLPAFMRVSSTANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWKAISDFCEKHGVDYLTGSWWPVLEDLYRSNIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLNAYQYQLALERFEWNEVKKVKSIVPMIHVSWNVARTIKITDPNTFKMIKHCLMQSIKHIQILRDQLVSAGKKISYQSRVKDEPAYYCNECDLEVFDLLFVTSENSSRKTYVVHCEDCARERSPGLNGVVVLEQYRIDELMNMYDNFTLSAVPCCR

Target ORF information:

RefSeq Version	XM_005172621.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172621.4

ORF Insert Sequence:

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6bb ( XM_021479186.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334861.1 Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X3, mRNA.

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CloneID	ODa16069
Clone ID Related Accession (Same CDS sequence)	NM_001030178.2 , XM_005172623.3 , XM_005172621.3 , XM_005172621.4 , XM_021479186.1 , XM_005172623.4
Accession Version	XM_021479186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine (K)-specific demethylase 6B, b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334861.1
CDS	237..5396
Translation	MYHIAEQYSGRNSWDSFPSSGPNRAPWAPGNNRHWAPPSSGGNYQSPSQNYMAGRSYPNKAYNNRPPGFSRDRLHAHPRDRGNGKEQRVPRSCGPTQNQHHNPERSTLNYSSGYEGHLSSSNNHGNQPHRYGGPHQQQRSTGRPPQTQGEKWTHSAQSRSFQGPSLKRPVPPHNLSQPLREPSPSRDDCPSKRSRSINSHQNFHPRDKYFINRPPPSHPPRPWSPAYKSSMSWSLSEKRSSPSRFQESSHSVTRDHTYRPYSHSSPPSSVPNQGLHNRKLTKQGPAHHPVHDHPDSPPRGAKGGWDCPSPANLTVPYSQQHQLPPPQRHTGPSSSPATSVPQPRSNTAQHGWKTQNRSGKHSNSEMGRTTYELDSHQSVGGDRKRSSGSKHISRQRSPIQPYNSAEGRVRDCTEWRNPETGLKKHDQKDSDSPSEPTTQTSERGQRAQRSEMKKSRHSKHQNKLKSTHKSALSKLDSELLKHIQAKKRHKERLRRKQKKEEGELTEDTKERIEKKIEERKKKKTREGRKGHDKKTIHKSGRKLGQVKKRAKEERKQGDRKSETSISKTASPNLGTLYPSDSDPLLDDLLADPLHLSYIEEENEFSQHTSGFILPCKSPVNNNHCSPCQSTLEINTKVLEVDEKSDSEPDHVDTSDDDSVGTHDVSGSNPSLLNTHVSEEEPIRCKEVVPGLHTAPDLLPAHCTYSEELLRLDPPTSPPVLSWQGSPVLDLGDDDEDVKEEDMIGVLRRPVLQPSPTHSSPLREAIEISTGVDYCHSDLAKLYGLPESSKAMDTEEEDEEKTDGEQKDGSPDASGSSETNKPHLHQMDTFKSPSSAMASHRYTYRGGPFGRPPPSALVGVKYSSSLSLGPEIHPPDQQSPPASSPTTEIPDQVIHSLIQPTDKDKESETEEEIMKTGAKEDKIEVKGEETKVQKMDEQMEVKLAEPTKDPASTSLEEKESVMSPATVQAKLVHSCELLLNQNSSPVSKVMKVSGSKSDQRENEGDRQKDQLKTPRKAIRTMKEEEPETCVGRSIKAKHHKKGDRGENKKDIMKDKAETNSSSLSSEDAQKQLVEIRTKQVVPENQQIEIKVGKTESHKIKREKETDVKKDAIKEDAIKADSSATGCTSCTTNLQSSSTMTLFTRPPMLERVNLRNQHELSKIPLKELKICLVKIESRGRQTFRASEIEEKSIPLDAINIRNNAAEVIRACKGADIKNKFRESYLLPSLSVKPDLSCRTPIPRDKLNPPTPSIYLESKRDAFSPVLLQFCTDSKNAVTVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWNHSGSAQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKDSEDEDEEDKSKEKNEAKSNSQNNIGTTSTTTSSKQKAVGKIIKFGTNIDLSDPKRWKAQLQELLKLPAFMRVSSTANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWKAISDFCEKHGVDYLTGSWWPVLEDLYRSNIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLNAYQYQLALERFEWNEVKKVKSIVPMIHVSWNVARTIKITDPNTFKMIKHCLMQSIKHIQILRDQLVSAGKKISYQSRVKDEPAYYCNECDLEVFDLLFVTSENSSRKTYVVHCEDCARERSPGLNGVVVLEQYRIDELMNMYDNFTLSAVPCCR

Target ORF information:

RefSeq Version	XM_021479186.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479186.1

ORF Insert Sequence:

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6bb ( XM_005172623.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172680.1 Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X2, mRNA.

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CloneID	ODa16069
Clone ID Related Accession (Same CDS sequence)	NM_001030178.2 , XM_005172623.3 , XM_005172621.3 , XM_005172621.4 , XM_021479186.1 , XM_005172623.4
Accession Version	XM_005172623.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine (K)-specific demethylase 6B, b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172623.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172680.1
CDS	325..5484
Translation	MYHIAEQYSGRNSWDSFPSSGPNRAPWAPGNNRHWAPPSSGGNYQSPSQNYMAGRSYPNKAYNNRPPGFSRDRLHAHPRDRGNGKEQRVPRSCGPTQNQHHNPERSTLNYSSGYEGHLSSSNNHGNQPHRYGGPHQQQRSTGRPPQTQGEKWTHSAQSRSFQGPSLKRPVPPHNLSQPLREPSPSRDDCPSKRSRSINSHQNFHPRDKYFINRPPPSHPPRPWSPAYKSSMSWSLSEKRSSPSRFQESSHSVTRDHTYRPYSHSSPPSSVPNQGLHNRKLTKQGPAHHPVHDHPDSPPRGAKGGWDCPSPANLTVPYSQQHQLPPPQRHTGPSSSPATSVPQPRSNTAQHGWKTQNRSGKHSNSEMGRTTYELDSHQSVGGDRKRSSGSKHISRQRSPIQPYNSAEGRVRDCTEWRNPETGLKKHDQKDSDSPSEPTTQTSERGQRAQRSEMKKSRHSKHQNKLKSTHKSALSKLDSELLKHIQAKKRHKERLRRKQKKEEGELTEDTKERIEKKIEERKKKKTREGRKGHDKKTIHKSGRKLGQVKKRAKEERKQGDRKSETSISKTASPNLGTLYPSDSDPLLDDLLADPLHLSYIEEENEFSQHTSGFILPCKSPVNNNHCSPCQSTLEINTKVLEVDEKSDSEPDHVDTSDDDSVGTHDVSGSNPSLLNTHVSEEEPIRCKEVVPGLHTAPDLLPAHCTYSEELLRLDPPTSPPVLSWQGSPVLDLGDDDEDVKEEDMIGVLRRPVLQPSPTHSSPLREAIEISTGVDYCHSDLAKLYGLPESSKAMDTEEEDEEKTDGEQKDGSPDASGSSETNKPHLHQMDTFKSPSSAMASHRYTYRGGPFGRPPPSALVGVKYSSSLSLGPEIHPPDQQSPPASSPTTEIPDQVIHSLIQPTDKDKESETEEEIMKTGAKEDKIEVKGEETKVQKMDEQMEVKLAEPTKDPASTSLEEKESVMSPATVQAKLVHSCELLLNQNSSPVSKVMKVSGSKSDQRENEGDRQKDQLKTPRKAIRTMKEEEPETCVGRSIKAKHHKKGDRGENKKDIMKDKAETNSSSLSSEDAQKQLVEIRTKQVVPENQQIEIKVGKTESHKIKREKETDVKKDAIKEDAIKADSSATGCTSCTTNLQSSSTMTLFTRPPMLERVNLRNQHELSKIPLKELKICLVKIESRGRQTFRASEIEEKSIPLDAINIRNNAAEVIRACKGADIKNKFRESYLLPSLSVKPDLSCRTPIPRDKLNPPTPSIYLESKRDAFSPVLLQFCTDSKNAVTVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWNHSGSAQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKDSEDEDEEDKSKEKNEAKSNSQNNIGTTSTTTSSKQKAVGKIIKFGTNIDLSDPKRWKAQLQELLKLPAFMRVSSTANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWKAISDFCEKHGVDYLTGSWWPVLEDLYRSNIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLNAYQYQLALERFEWNEVKKVKSIVPMIHVSWNVARTIKITDPNTFKMIKHCLMQSIKHIQILRDQLVSAGKKISYQSRVKDEPAYYCNECDLEVFDLLFVTSENSSRKTYVVHCEDCARERSPGLNGVVVLEQYRIDELMNMYDNFTLSAVPCCR

Target ORF information:

RefSeq Version	XM_005172623.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, b (kdm6bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172623.4

ORF Insert Sequence:

ATGTACCACA TAGCAGAGCA GTATTCTGGA CGTAACTCGT GGGACTCCTT CCCCTCTTCT 
GGGCCGAATC GAGCACCATG GGCCCCAGGC AACAACCGGC ACTGGGCCCC TCCATCCAGC 
GGAGGGAACT ATCAATCCCC ATCACAAAAC TACATGGCAG GAAGAAGCTA CCCAAACAAA 
GCTTATAACA ACAGACCTCC AGGTTTCAGC AGGGACAGAC TCCATGCTCA CCCCAGAGAC 
CGAGGCAATG GAAAGGAGCA GAGGGTTCCT AGGAGCTGTG GACCAACCCA GAACCAGCAC 
CACAACCCAG AACGCTCTAC CCTAAACTAC AGCTCAGGGT ATGAAGGACA CCTCTCTAGC 
AGTAACAATC ATGGCAACCA GCCACACAGG TATGGGGGTC CGCACCAGCA GCAGCGTTCC 
ACTGGGCGCC CCCCACAGAC CCAGGGAGAG AAGTGGACAC ATTCAGCTCA ATCCAGGTCT 
TTCCAAGGGC CCTCTCTCAA ACGTCCAGTA CCTCCTCACA ATCTCTCACA ACCCCTCCGT 
GAACCGTCCC CTTCACGAGA TGACTGTCCT AGCAAGAGGA GTAGGAGCAT CAACTCACAC 
CAGAATTTCC ACCCTAGAGA CAAATATTTC ATCAACCGTC CACCTCCATC TCATCCACCT 
CGTCCCTGGA GCCCAGCATA CAAAAGCTCA ATGTCCTGGA GCCTGTCTGA GAAGAGGTCC 
TCCCCCTCCC GCTTTCAGGA GTCCAGTCAT TCAGTCACAA GAGATCACAC CTATAGACCA 
TATTCCCACT CATCACCACC CAGCAGTGTC CCAAACCAGG GCCTCCACAA CAGGAAACTC 
ACCAAACAAG GGCCCGCCCA TCATCCGGTC CATGATCATC CAGACTCCCC TCCCCGCGGG 
GCAAAAGGGG GCTGGGATTG CCCATCACCA GCAAACCTCA CTGTGCCTTA CAGTCAGCAG 
CACCAGCTCC CTCCACCACA GAGGCACACC GGCCCCTCCA GCAGCCCAGC CACCTCAGTA 
CCTCAACCCA GATCCAACAC AGCACAGCAT GGCTGGAAAA CGCAGAACCG CTCTGGAAAA 
CACAGTAACA GCGAGATGGG CCGTACAACC TATGAACTGG ACTCCCATCA GTCTGTGGGT 
GGGGACAGAA AACGATCAAG CGGATCCAAA CACATTAGCC GTCAACGAAG TCCTATCCAA 
CCATACAACA GTGCTGAGGG TAGAGTCAGA GATTGTACTG AATGGAGGAA CCCAGAGACT 
GGGTTAAAGA AACATGACCA GAAAGACTCA GACTCTCCTT CTGAGCCTAC CACTCAAACC 
TCAGAAAGAG GTCAGAGGGC ACAGAGGTCA GAAATGAAGA AAAGCAGGCA CTCTAAACAC 
CAGAATAAAT TAAAATCCAC CCACAAGAGT GCCCTCAGCA AGCTGGACTC TGAACTCTTG 
AAACACATTC AGGCCAAGAA GAGGCACAAG GAGCGTTTGA GGAGGAAGCA GAAAAAGGAG 
GAAGGAGAAC TGACAGAAGA CACAAAGGAA CGGATAGAGA AAAAAATAGA AGAAAGGAAA 
AAGAAAAAGA CGAGGGAAGG AAGAAAAGGC CATGATAAGA AAACCATACA TAAAAGTGGG 
AGAAAGTTAG GGCAGGTTAA AAAAAGAGCA AAAGAGGAAA GAAAACAAGG AGACAGAAAA 
AGTGAGACAT CCATTTCAAA AACAGCATCT CCTAATTTGG GAACTTTGTA TCCCAGTGAT 
TCTGATCCTC TCTTGGATGA CCTGCTGGCT GATCCACTTC ATCTCAGTTA CATAGAGGAA 
GAGAACGAGT TCTCTCAACA TACCTCAGGG TTCATCCTAC CTTGTAAATC TCCTGTCAAC 
AATAACCATT GTTCTCCATG CCAATCAACC CTTGAAATCA ATACTAAAGT CTTGGAGGTT 
GATGAAAAGT CAGATTCTGA GCCTGACCAT GTAGACACTA GCGATGATGA CAGTGTAGGA 
ACCCATGATG TTAGTGGATC CAATCCCTCT CTTCTTAATA CACATGTCTC TGAAGAAGAA 
CCCATCAGAT GCAAGGAGGT TGTCCCAGGG TTGCATACTG CTCCAGACCT CCTACCGGCT 
CACTGCACCT ATAGCGAGGA ACTCCTAAGG TTGGACCCAC CTACAAGCCC ACCTGTTTTA 
AGCTGGCAGG GTTCTCCTGT TTTAGACTTG GGTGATGATG ATGAGGATGT GAAAGAAGAA 
GATATGATTG GAGTGTTGAG AAGGCCAGTG CTTCAACCTA GTCCCACACA CTCATCTCCA 
CTTAGAGAAG CAATTGAAAT CAGCACTGGG GTTGATTATT GCCACAGTGA CCTGGCCAAG 
TTGTATGGAC TTCCTGAGTC CTCTAAAGCA ATGGACACTG AGGAGGAGGA TGAAGAAAAG 
ACAGATGGGG AGCAAAAAGA TGGTAGTCCT GATGCATCAG GAAGTTCTGA GACCAACAAA 
CCACATTTGC ACCAAATGGA CACATTCAAA TCTCCATCCT CAGCAATGGC CAGCCATAGA 
TATACTTATA GGGGTGGGCC ATTTGGTCGG CCACCCCCTA GTGCTTTAGT TGGGGTCAAA 
TACTCCTCAT CTCTGTCCTT GGGTCCTGAG ATCCACCCAC CAGACCAGCA GAGTCCACCT 
GCCTCATCCC CAACCACAGA GATTCCAGAT CAGGTTATAC ATTCGCTGAT CCAGCCCACA 
GACAAGGACA AAGAAAGTGA AACAGAAGAA GAAATAATGA AAACTGGGGC AAAAGAGGAC 
AAAATAGAAG TAAAAGGAGA GGAAACTAAG GTACAGAAGA TGGATGAACA AATGGAGGTA 
AAATTAGCCG AACCAACTAA AGACCCAGCC AGCACATCAC TGGAAGAGAA AGAATCTGTG 
ATGTCTCCAG CCACTGTTCA AGCTAAACTT GTACACAGCT GTGAACTACT GCTCAATCAA 
AACTCCAGCC CAGTGTCAAA GGTCATGAAA GTCAGTGGAT CCAAGTCTGA TCAGAGAGAA 
AATGAAGGAG ACAGACAGAA AGATCAGTTG AAGACTCCAA GAAAAGCGAT TAGGACCATG 
AAAGAAGAAG AACCTGAAAC TTGTGTTGGT AGAAGCATTA AAGCAAAACA TCATAAAAAA 
GGTGACAGAG GAGAGAATAA AAAGGACATA ATGAAAGATA AGGCTGAGAC AAATTCTTCT 
TCTTTGTCTT CTGAAGATGC ACAGAAGCAA TTGGTGGAAA TCAGGACCAA ACAAGTTGTC 
CCAGAAAACC AACAAATTGA AATCAAAGTT GGAAAAACCG AATCTCATAA AATTAAGAGA 
GAAAAAGAGA CAGATGTTAA AAAAGATGCC ATAAAAGAAG ACGCTATTAA GGCTGATTCA 
TCCGCTACAG GATGTACATC TTGCACAACT AACCTGCAAT CCTCTAGTAC TATGACTCTC 
TTCACCAGAC CACCAATGCT GGAGCGGGTA AATCTCCGAA ATCAACACGA GCTCTCTAAA 
ATTCCCTTAA AAGAACTAAA GATCTGTTTG GTCAAGATTG AGAGCAGAGG TCGGCAGACT 
TTCAGAGCCT CAGAGATTGA GGAGAAAAGC ATTCCACTTG ATGCCATCAA CATCAGAAAC 
AATGCAGCCG AGGTCATACG AGCTTGCAAA GGGGCAGACA TCAAGAACAA GTTTCGTGAA 
TCATACCTGC TCCCGTCGCT TTCAGTGAAA CCTGACCTGT CATGCAGGAC CCCAATTCCC 
AGAGATAAAC TGAACCCTCC TACACCAAGC ATTTATCTGG AGAGTAAAAG GGATGCTTTC 
TCTCCCGTGC TCCTGCAGTT CTGCACGGAC TCTAAAAATG CAGTTACTGT AATCAGAGGA 
CTGGCTGGAT CCCTCCGTCT CAATCTGGGT TTGTTCTCCA CAAAATCTCT GGTGGAAGCC 
AACTCTGAGC ATGCAGTGGA GGTGAGGACC CAGGTGCAGC AGCCTGCAGA TGAGAACTGG 
AACCATTCAG GATCCGCCCA GACTTGGCCC TGCGAAAGTA GCAGATCTCA CACCACCATT 
GCTAAATACG CACAGTATCA AGCATCCAGT TTCCAGGAGA GCCTACAAGA AGAGAAAGAC 
AGTGAAGATG AAGATGAGGA AGACAAATCA AAGGAAAAAA ATGAGGCAAA ATCCAACTCA 
CAAAACAACA TTGGGACAAC TTCAACCACA ACCAGCTCAA AGCAGAAAGC TGTTGGGAAA 
ATTATTAAAT TTGGAACCAA CATTGACTTA TCTGACCCAA AACGGTGGAA GGCGCAGTTG 
CAGGAGCTGT TGAAGTTGCC TGCGTTCATG CGTGTGTCCT CCACCGCAAA CATGCTGAGT 
CATGTTGGCC ACACTATACT GGGCATGAAC ACTGTTCAGC TTTATATGAA GGTCCCTGGC 
AGCCGCACAC CAGGACATCA GGAAAATAAT AACTTCTGTT CAGTGAATAT CAATATTGGC 
CCTGGTGACT GTGAATGGTT TGCTGTCCAT GAACACTACT GGAAAGCCAT CAGTGACTTC 
TGTGAAAAGC ATGGCGTGGA CTATCTGACA GGGTCATGGT GGCCGGTTCT GGAGGACCTT 
TACCGTTCCA ACATCCCTGT GTACCGCTTC ATTCAGAGGC CAGGTGACCT GGTGTGGATC 
AACGCTGGCA CTGTGCACTG GGTCCAGGCT GTGGGCTGGT GCAACAACAT CGCCTGGAAT 
GTGGGCCCTT TAAATGCCTA TCAATACCAG CTAGCTCTTG AGCGCTTTGA ATGGAACGAG 
GTCAAGAAGG TCAAATCAAT CGTCCCTATG ATTCACGTGT CCTGGAACGT TGCCCGCACA 
ATCAAAATCA CAGACCCAAA CACATTCAAG ATGATCAAAC ATTGTCTCAT GCAGTCCATC 
AAACACATTC AGATCTTGAG GGACCAGCTG GTGTCTGCAG GGAAGAAGAT CTCCTATCAG 
AGTAGGGTGA AGGATGAACC GGCGTATTAC TGCAATGAGT GTGATTTGGA GGTGTTTGAC 
CTGCTGTTTG TGACTAGTGA GAACAGCAGT AGGAAAACAT ATGTGGTTCA CTGTGAGGAC 
TGCGCTCGGG AACGAAGCCC CGGTCTGAAC GGGGTGGTGG TGCTGGAGCA GTACCGCATC 
GATGAGCTGA TGAACATGTA TGATAACTTT ACCCTGTCTG CAGTTCCATG CTGTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation.
Developmental biology426(1)84-96(2017 06)
Akerberg AA,Henner A,Stewart S,Stankunas K

Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
BMC genomics17259(2016 Mar)
Brocal I,White RJ,Dooley CM,Carruthers SN,Clark R,Hall A,Busch-Nentwich EM,Stemple DL,Kettleborough RN

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kdm6bb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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