flnb cDNA ORF clone, Danio rerio(zebrafish)

The following flnb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the flnb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa146814 XM_021474538.1
Latest version!
Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa151550 XM_021469934.1
Latest version!
Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa146811 XM_021474535.1
Latest version!
Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa146812 XM_021474536.1
Latest version!
Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa146813 XM_021474537.1
Latest version!
Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa27643 XM_021469933.1
Latest version!
Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa27643 XM_686387.7
Latest version!
Danio rerio filamin B, beta (actin binding protein 278) (flnb), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa146814
    Clone ID Related Accession (Same CDS sequence) XM_021474538.1
    Accession Version XM_021474538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filamin-B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG
    CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT
    CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA
    AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT
    GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT
    GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT
    TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC
    AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG
    AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA
    AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA
    GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT
    GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT
    GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA
    GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG
    GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA
    GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT
    GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA
    TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT
    GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT
    GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA
    GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC
    AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT
    CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA
    ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT
    GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT
    TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC
    AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT
    AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT
    ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC
    ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA
    CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC
    AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT
    CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT
    GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC
    AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC
    GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA
    AGTATTCACA GGTTTAATGC CATTCCTCCT CACTCTGCCA GAAAGGATAA AGACATTGGA
    AATAAAAAGG AAAATTCGAG TCAGGAGGGA AATGTCCACT CTAAGCCTCC ACATACATTC
    ACTAACGGCT GCCGTGGGGA ACCAGAAGCT AATGCTAAAG CTAATGGCTC AATAAAAGTC
    GCAGATATAA TGGACAACAG GGATAAAAAA TGTGAGATGC CTTGCACGCC AAAAATGTCA
    GGCTGTAAGT CTGTAATGGA CAAATACAAC AGCAAAGAAG CTTTCAGAAG CCTCCAGACA
    GATCCGACTG TAAATACAGA GCAATCAGTC TGTGCTCAAA GTCCTCAGTT GTTCAGGATC
    AGCTGTAGTA AATCCGGGAA GCCCCCTGAC TGTGTGCTGA TGATCAGACC TGATGGAGAG
    ACTGAACTGA CAGAGGTCAA AGAAAACCCT GACGGGACTT ACTCTGTCAA ATACTGTCCC
    ACTAATGAAG GTCTTCATTC CTTGATGGTC AAATATGTTG ATGATGATTC ATTTTACAAT
    CCTTTGAGGT TTCATGTCCT TCCTCACGCT GATAGTCATG ACAAACATGT GATTAATACA
    ACTGAAGCTT GTAGCAGACA ATTAGAGGAA CGTGTCAGCG CTCAAATTCC TCAGACTTTC
    ACAACTTGCT GCAACACTAA CGAGGCCTCA GGGACCGAGA TGTTGAAAGC TGATGATCAT
    GTTGAAGTGT TAAATAATGA GAACACAACC AGTGAAACAA CACACACACC TGAACCCTCA
    GCGGTCAGAA ATGCCACACT GGAGGATTTT CAAAGGCACC AGCCACCATC CTCTCACACT
    TCAAGTGGGT ACAGGGGTTT TCAAAACAAG GTTGAGGGCA GGAATGCAAT CCCTCCTGCT
    GCCATTAATC AAGCAGGGTT TATATCCTGT GATATAGTCC TGCCAGGAGA CTTAAGTGAA
    GGTGAAATTA CAGGAGAGGT GATCACGCCG TCTGGAAAGA GAGCACAGCC TGACATGATT
    GATAATAAAG ATGGGACTGT CGTTATGAAG TTTGATCCTT CAGAGGAGGG TCTTCATCAA
    ATCCTTATTA AATCAGCAGA AAATGATATA CCAGAACTTC CACTGCAATA TTATGCAAAT
    AGTTTGGCTA ATCGCAGCCC TGTGGCCTAT GGGCGTGGCC TTGTGTGTGG AATTGCAAAT
    GAGACTGCAA CATTCACCAT CTGTCAGGAG GATTCAGCCT CGAGTGAACT GGACATCACA
    ATTGAGGGTC CGTCTGAGGC TGATGTGCAC TGCTTGGACA ATGAAGATGG CACTTGTTCT
    GTGAGTTACC TTCCAACTGA GCCTGGAGAT TATGAGATAC AAGTTCAACA TAATGACGTG
    CCTATTCCAG GCAGTCCTTT CAAGGCCAAA ATTACAGTTT TTATAAACCC AATACAGATG
    GACATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330213.1
    CDS996..6224
    Translation

    Target ORF information:

    RefSeq Version XM_021474538.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG
    CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT
    CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA
    AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT
    GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT
    GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT
    TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC
    AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG
    AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA
    AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA
    GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT
    GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT
    GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA
    GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG
    GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA
    GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT
    GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA
    TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT
    GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT
    GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA
    GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC
    AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT
    CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA
    ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT
    GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT
    TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC
    AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT
    AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT
    ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC
    ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA
    CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC
    AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT
    CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT
    GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC
    AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC
    GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA
    AGTATTCACA GGTTTAATGC CATTCCTCCT CACTCTGCCA GAAAGGATAA AGACATTGGA
    AATAAAAAGG AAAATTCGAG TCAGGAGGGA AATGTCCACT CTAAGCCTCC ACATACATTC
    ACTAACGGCT GCCGTGGGGA ACCAGAAGCT AATGCTAAAG CTAATGGCTC AATAAAAGTC
    GCAGATATAA TGGACAACAG GGATAAAAAA TGTGAGATGC CTTGCACGCC AAAAATGTCA
    GGCTGTAAGT CTGTAATGGA CAAATACAAC AGCAAAGAAG CTTTCAGAAG CCTCCAGACA
    GATCCGACTG TAAATACAGA GCAATCAGTC TGTGCTCAAA GTCCTCAGTT GTTCAGGATC
    AGCTGTAGTA AATCCGGGAA GCCCCCTGAC TGTGTGCTGA TGATCAGACC TGATGGAGAG
    ACTGAACTGA CAGAGGTCAA AGAAAACCCT GACGGGACTT ACTCTGTCAA ATACTGTCCC
    ACTAATGAAG GTCTTCATTC CTTGATGGTC AAATATGTTG ATGATGATTC ATTTTACAAT
    CCTTTGAGGT TTCATGTCCT TCCTCACGCT GATAGTCATG ACAAACATGT GATTAATACA
    ACTGAAGCTT GTAGCAGACA ATTAGAGGAA CGTGTCAGCG CTCAAATTCC TCAGACTTTC
    ACAACTTGCT GCAACACTAA CGAGGCCTCA GGGACCGAGA TGTTGAAAGC TGATGATCAT
    GTTGAAGTGT TAAATAATGA GAACACAACC AGTGAAACAA CACACACACC TGAACCCTCA
    GCGGTCAGAA ATGCCACACT GGAGGATTTT CAAAGGCACC AGCCACCATC CTCTCACACT
    TCAAGTGGGT ACAGGGGTTT TCAAAACAAG GTTGAGGGCA GGAATGCAAT CCCTCCTGCT
    GCCATTAATC AAGCAGGGTT TATATCCTGT GATATAGTCC TGCCAGGAGA CTTAAGTGAA
    GGTGAAATTA CAGGAGAGGT GATCACGCCG TCTGGAAAGA GAGCACAGCC TGACATGATT
    GATAATAAAG ATGGGACTGT CGTTATGAAG TTTGATCCTT CAGAGGAGGG TCTTCATCAA
    ATCCTTATTA AATCAGCAGA AAATGATATA CCAGAACTTC CACTGCAATA TTATGCAAAT
    AGTTTGGCTA ATCGCAGCCC TGTGGCCTAT GGGCGTGGCC TTGTGTGTGG AATTGCAAAT
    GAGACTGCAA CATTCACCAT CTGTCAGGAG GATTCAGCCT CGAGTGAACT GGACATCACA
    ATTGAGGGTC CGTCTGAGGC TGATGTGCAC TGCTTGGACA ATGAAGATGG CACTTGTTCT
    GTGAGTTACC TTCCAACTGA GCCTGGAGAT TATGAGATAC AAGTTCAACA TAATGACGTG
    CCTATTCCAG GCAGTCCTTT CAAGGCCAAA ATTACAGTTT TTATAAACCC AATACAGATG
    GACATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151550
    Clone ID Related Accession (Same CDS sequence) XM_021469934.1
    Accession Version XM_021469934.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filamin-C isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG
    CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT
    GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC
    ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG
    TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC
    AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC
    CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA
    TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC
    TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG
    GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC
    GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT
    GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG
    GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC
    GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC
    ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA
    ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT
    GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG
    AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC
    TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA
    ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC
    AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA
    GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC
    GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA
    ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA
    TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA
    CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT
    AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT
    CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA
    AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG
    GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT
    TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA
    GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC
    TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT
    GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT
    TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT
    CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT
    GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG
    AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG
    GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC
    AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG
    GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA
    GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG
    AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC
    AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT
    TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC
    CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA
    CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT
    AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT
    GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA
    CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT
    TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG
    TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG
    GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT
    GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA
    GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC
    CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC
    ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG
    GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT
    GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT
    TTCAAGGCCA AAATTACAGT TTTTATAAAC CCAATACAGA TGGACATATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325609.1
    CDS559..5769
    Translation

    Target ORF information:

    RefSeq Version XM_021469934.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG
    CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT
    GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC
    ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG
    TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC
    AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC
    CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA
    TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC
    TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG
    GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC
    GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT
    GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG
    GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC
    GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC
    ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA
    ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT
    GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG
    AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC
    TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA
    ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC
    AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA
    GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC
    GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA
    ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA
    TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA
    CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT
    AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT
    CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA
    AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG
    GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT
    TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA
    GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC
    TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT
    GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT
    TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT
    CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT
    GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG
    AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG
    GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC
    AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG
    GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA
    GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG
    AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC
    AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT
    TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC
    CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA
    CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT
    AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT
    GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA
    CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT
    TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG
    TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG
    GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT
    GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA
    GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC
    CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC
    ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG
    GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT
    GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT
    TTCAAGGCCA AAATTACAGT TTTTATAAAC CCAATACAGA TGGACATATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146811
    Clone ID Related Accession (Same CDS sequence) XM_021474535.1
    Accession Version XM_021474535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filamin-A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG
    CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT
    CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA
    AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT
    GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT
    GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT
    TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC
    AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG
    AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA
    AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA
    GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT
    GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT
    GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA
    GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG
    GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA
    GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT
    GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA
    TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT
    GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT
    GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA
    GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC
    AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT
    CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA
    ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT
    GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT
    TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC
    AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT
    AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT
    ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC
    ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA
    CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC
    AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT
    CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT
    GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC
    AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC
    GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA
    AGTATTCACA GGTTTAATGC CATTCCTCCT CACTCTGCCA GAAAGGATAA AGACATTGGA
    AATAAAAAGG AAAATTCGAG TCAGGAGGGA AATGTCCACT CTAAGCCTCC ACATACATTC
    ACTAACGGCT GCCGTGGGGA ACCAGAAGCT AATGCTAAAG CTAATGGCTC AATAAAAGTC
    GCAGATATAA TGGACAACAG GGATAAAAAA TGTGAGATGC CTTGCACGCC AAAAATGTCA
    GGCTGTAAGT CTGTAATGGA CAAATACAAC AGCAAAGAAG CTTTCAGAAG CCTCCAGACA
    GATCCGACTG TAAATACAGA GCAATCAGTC TGTGCTCAAA GTCCTCAGTT GTTCAGGATC
    AGCTGTAGTA AATCCGGGAA GCCCCCTGAC TGTGTGCTGA TGATCAGACC TGATGGAGAG
    ACTGAACTGA CAGAGGTCAA AGAAAACCCT GACGGGACTT ACTCTGTCAA ATACTGTCCC
    ACTAATGAAG GTCTTCATTC CTTGATGGTC AAATATGTTG ATGATGATTC ATTTTACAAT
    CCTTTGAGGT TTCATGTCCT TCCTCACGCT GATAGTCATG ACAAACATGT GATTAATACA
    ACTGAAGCTT GTAGCAGACA ATTAGAGGAA CGTGTCAGCG CTCAAATTCC TCAGACTTTC
    ACAACTTGCT GCAACACTAA CGAGGCCTCA GGGACCGAGA TGTTGAAAGC TGATGATCAT
    GTTGAAGTGT TAAATAATGA GAACACAACC AGTGAAACAA CACACACACC TGAACCCTCA
    GCGGTCAGAA ATGCCACACT GGAGGATTTT CAAAGGCACC AGCCACCATC CTCTCACACT
    TCAAGTGGGT ACAGGGGTTT TCAAAACAAG GTTGAGGGCA GGAATGCAAT CCCTCCTGCT
    GCCATTAATC AAGCAGGGTT TATATCCTGT GATATAGTCC TGCCAGGAGA CTTAAGTGAA
    GGTGAAATTA CAGGAGAGGT GATCACGCCG TCTGGAAAGA GAGCACAGCC TGACATGATT
    GATAATAAAG ATGGGACTGT CGTTATGAAG TTTGATCCTT CAGAGGAGGG TCTTCATCAA
    ATCCTTATTA AATCAGCAGA AAATGATATA CCAGAACTTC CACTGCAATA TTATGCAAAT
    AGTTTGGCTA ATCGCAGCCC TGTGGCCTAT GGGCGTGGCC TTGTGTGTGG AATTGCAAAT
    GAGACTGCAA CATTCACCAT CTGTCAGGAG GATTCAGCCT CGAGTGAACT GGACATCACA
    ATTGAGGGTC CGTCTGAGGC TGATGTGCAC TGCTTGGACA ATGAAGATGG CACTTGTTCT
    GTGAGTTACC TTCCAACTGA GCCTGGAGAT TATGAGATAC AAGTTCAACA TAATGACGTG
    CCTATTCCAG GCAGTCCTTT CAAGGCCAAA ATTACAGATG GACATATGAG AAAGTCAGAG
    GTCAAACTGG GCAGTGCGGT CGATTTTACC CTGGACATCA CAGAAGAAGA CATTAGCCAG
    CTCTCCGCTA GCATTATGTC GCCCACAGGC AATGATGTAC CCTGCTTACT GAAAACACAA
    GCTGACAGCC ATCTCGGCGT CTCCTTCATC CCCAGAGAGG CTGGTGAGCA TCTGGTTAGC
    ATCATGAAGG ATGGGGAGCA TGTGAGCAAC AGTCCCATCT CTCTGTCCAT CAGTCAGGCA
    GAGATTGGAG ATGCCAGTAA GGTGAAGGCT TTCGGTCCAG GCTTACACAC CGGGCACACC
    TTCTGCAAGT CTGAATTTGT GGTGGACACT TGGGATGCAG GATATGGTGG GTTAACAGTT
    GTAATTGAAG GTCCCAGTAA AGTAGACGTC CACACAGAAG AGCTGGAGAA TGATGGAACT
    TGCAATATAT CATACTGCCC AATCAAGCCT GGCAGCTATA AAATCTCCAT CAAATTCTCA
    GATGAGCACA TTCCAGGGAG TCCGTTTACA GCTGTGGTGA AAGACCAAGG GCTTATGAGA
    GAGAACATCA CATACAACCA GAAAGCTGCT CCTACTGCCA GCATTGGGAG TGAATGCAGT
    TTAGCCTTCA AACTCCCAGA TGTTGATCCT GGGACTATGT CTGCTCATGT ATATGAACCA
    TCAGGAAGTT TTGGAGAGGC TGTTATCCTT CCTAGAACAG GCCGTGACAT CTATGTAGTG
    AGCTTTCTAG CTAAAGAGAT GGGAGTTTAT AATGTGAGCA TACAGAACAA AGGCCAGACG
    ATTTCCGGCT GCCCACTTCA ATACACCGTC GGCCCGCTGG GACGAGGAGG ATCTGAGAAG
    GTGCAGGTTT GGGGACAAAG CCTTCAGACA GCTCTGGCAA CTGTTCCAGC TGACTTTAAT
    ATTTGGTCGC GTGAGGCTGG AGCAGGGGCT CTGTCTGTCT CCGTAGAGGG TCCTGGAGAG
    ATTGAGCTCC ACTTTGATGA GCAGATGGAC GGGTCTTGCA TTGTGACCTA TACTGCACAA
    CAGCCTGGTG ACTATGAGGT TTCAGTCATG TTTAATGAGG ATCATGTTTC CCAAAGCCCT
    TTTCTTGTGT CTGTCATTGC CGCCACAGAC ACTGCTGTCT CTGATCAAAC TCAGCTGTGT
    AACGGTCATG GAGAAGCCTC TCTGTCAGGA CCGCAGATCA ACACAACATC TGACAGCAGT
    GCTGAGCCAG TTTTGCTGTC TGACGCTAGT AAAGTCATCT GCCACGGCCC AGGCCTGAGT
    AAGGGCTTTC TGGGACGCAA AAACACTTTT TATGTGGACT GCAGTAAAGC TGGACGAAAC
    CTTCTGTTCG TGGGCATGCA TGGCCCTACA GTTCCCTGTG AGCGGGTGTC CATCATTCAC
    ATGGGCCAGT GTCAGTACAG GATCAACTAC GCTGTAAAGG AAAGAGGAAA ATATGTCTTA
    GCAGTGAAGT GGGGAGATGA GCACATTCCT GGATCGCCGT TTTACATTAG TGTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330210.1
    CDS996..7715
    Translation

    Target ORF information:

    RefSeq Version XM_021474535.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG
    CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT
    CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA
    AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT
    GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT
    GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT
    TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC
    AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG
    AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA
    AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA
    GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT
    GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT
    GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA
    GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG
    GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA
    GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT
    GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA
    TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT
    GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT
    GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA
    GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC
    AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT
    CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA
    ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT
    GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT
    TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC
    AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT
    AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT
    ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC
    ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA
    CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC
    AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT
    CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT
    GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC
    AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC
    GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA
    AGTATTCACA GGTTTAATGC CATTCCTCCT CACTCTGCCA GAAAGGATAA AGACATTGGA
    AATAAAAAGG AAAATTCGAG TCAGGAGGGA AATGTCCACT CTAAGCCTCC ACATACATTC
    ACTAACGGCT GCCGTGGGGA ACCAGAAGCT AATGCTAAAG CTAATGGCTC AATAAAAGTC
    GCAGATATAA TGGACAACAG GGATAAAAAA TGTGAGATGC CTTGCACGCC AAAAATGTCA
    GGCTGTAAGT CTGTAATGGA CAAATACAAC AGCAAAGAAG CTTTCAGAAG CCTCCAGACA
    GATCCGACTG TAAATACAGA GCAATCAGTC TGTGCTCAAA GTCCTCAGTT GTTCAGGATC
    AGCTGTAGTA AATCCGGGAA GCCCCCTGAC TGTGTGCTGA TGATCAGACC TGATGGAGAG
    ACTGAACTGA CAGAGGTCAA AGAAAACCCT GACGGGACTT ACTCTGTCAA ATACTGTCCC
    ACTAATGAAG GTCTTCATTC CTTGATGGTC AAATATGTTG ATGATGATTC ATTTTACAAT
    CCTTTGAGGT TTCATGTCCT TCCTCACGCT GATAGTCATG ACAAACATGT GATTAATACA
    ACTGAAGCTT GTAGCAGACA ATTAGAGGAA CGTGTCAGCG CTCAAATTCC TCAGACTTTC
    ACAACTTGCT GCAACACTAA CGAGGCCTCA GGGACCGAGA TGTTGAAAGC TGATGATCAT
    GTTGAAGTGT TAAATAATGA GAACACAACC AGTGAAACAA CACACACACC TGAACCCTCA
    GCGGTCAGAA ATGCCACACT GGAGGATTTT CAAAGGCACC AGCCACCATC CTCTCACACT
    TCAAGTGGGT ACAGGGGTTT TCAAAACAAG GTTGAGGGCA GGAATGCAAT CCCTCCTGCT
    GCCATTAATC AAGCAGGGTT TATATCCTGT GATATAGTCC TGCCAGGAGA CTTAAGTGAA
    GGTGAAATTA CAGGAGAGGT GATCACGCCG TCTGGAAAGA GAGCACAGCC TGACATGATT
    GATAATAAAG ATGGGACTGT CGTTATGAAG TTTGATCCTT CAGAGGAGGG TCTTCATCAA
    ATCCTTATTA AATCAGCAGA AAATGATATA CCAGAACTTC CACTGCAATA TTATGCAAAT
    AGTTTGGCTA ATCGCAGCCC TGTGGCCTAT GGGCGTGGCC TTGTGTGTGG AATTGCAAAT
    GAGACTGCAA CATTCACCAT CTGTCAGGAG GATTCAGCCT CGAGTGAACT GGACATCACA
    ATTGAGGGTC CGTCTGAGGC TGATGTGCAC TGCTTGGACA ATGAAGATGG CACTTGTTCT
    GTGAGTTACC TTCCAACTGA GCCTGGAGAT TATGAGATAC AAGTTCAACA TAATGACGTG
    CCTATTCCAG GCAGTCCTTT CAAGGCCAAA ATTACAGATG GACATATGAG AAAGTCAGAG
    GTCAAACTGG GCAGTGCGGT CGATTTTACC CTGGACATCA CAGAAGAAGA CATTAGCCAG
    CTCTCCGCTA GCATTATGTC GCCCACAGGC AATGATGTAC CCTGCTTACT GAAAACACAA
    GCTGACAGCC ATCTCGGCGT CTCCTTCATC CCCAGAGAGG CTGGTGAGCA TCTGGTTAGC
    ATCATGAAGG ATGGGGAGCA TGTGAGCAAC AGTCCCATCT CTCTGTCCAT CAGTCAGGCA
    GAGATTGGAG ATGCCAGTAA GGTGAAGGCT TTCGGTCCAG GCTTACACAC CGGGCACACC
    TTCTGCAAGT CTGAATTTGT GGTGGACACT TGGGATGCAG GATATGGTGG GTTAACAGTT
    GTAATTGAAG GTCCCAGTAA AGTAGACGTC CACACAGAAG AGCTGGAGAA TGATGGAACT
    TGCAATATAT CATACTGCCC AATCAAGCCT GGCAGCTATA AAATCTCCAT CAAATTCTCA
    GATGAGCACA TTCCAGGGAG TCCGTTTACA GCTGTGGTGA AAGACCAAGG GCTTATGAGA
    GAGAACATCA CATACAACCA GAAAGCTGCT CCTACTGCCA GCATTGGGAG TGAATGCAGT
    TTAGCCTTCA AACTCCCAGA TGTTGATCCT GGGACTATGT CTGCTCATGT ATATGAACCA
    TCAGGAAGTT TTGGAGAGGC TGTTATCCTT CCTAGAACAG GCCGTGACAT CTATGTAGTG
    AGCTTTCTAG CTAAAGAGAT GGGAGTTTAT AATGTGAGCA TACAGAACAA AGGCCAGACG
    ATTTCCGGCT GCCCACTTCA ATACACCGTC GGCCCGCTGG GACGAGGAGG ATCTGAGAAG
    GTGCAGGTTT GGGGACAAAG CCTTCAGACA GCTCTGGCAA CTGTTCCAGC TGACTTTAAT
    ATTTGGTCGC GTGAGGCTGG AGCAGGGGCT CTGTCTGTCT CCGTAGAGGG TCCTGGAGAG
    ATTGAGCTCC ACTTTGATGA GCAGATGGAC GGGTCTTGCA TTGTGACCTA TACTGCACAA
    CAGCCTGGTG ACTATGAGGT TTCAGTCATG TTTAATGAGG ATCATGTTTC CCAAAGCCCT
    TTTCTTGTGT CTGTCATTGC CGCCACAGAC ACTGCTGTCT CTGATCAAAC TCAGCTGTGT
    AACGGTCATG GAGAAGCCTC TCTGTCAGGA CCGCAGATCA ACACAACATC TGACAGCAGT
    GCTGAGCCAG TTTTGCTGTC TGACGCTAGT AAAGTCATCT GCCACGGCCC AGGCCTGAGT
    AAGGGCTTTC TGGGACGCAA AAACACTTTT TATGTGGACT GCAGTAAAGC TGGACGAAAC
    CTTCTGTTCG TGGGCATGCA TGGCCCTACA GTTCCCTGTG AGCGGGTGTC CATCATTCAC
    ATGGGCCAGT GTCAGTACAG GATCAACTAC GCTGTAAAGG AAAGAGGAAA ATATGTCTTA
    GCAGTGAAGT GGGGAGATGA GCACATTCCT GGATCGCCGT TTTACATTAG TGTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146812
    Clone ID Related Accession (Same CDS sequence) XM_021474536.1
    Accession Version XM_021474536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filamin-A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG
    CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT
    CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA
    AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT
    GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT
    GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT
    TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC
    AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG
    AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA
    AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA
    GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT
    GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT
    GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA
    GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG
    GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA
    GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT
    GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA
    TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT
    GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT
    GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA
    GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC
    AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT
    CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA
    ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT
    GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT
    TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC
    AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT
    AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT
    ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC
    ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA
    CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC
    AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT
    CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT
    GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC
    AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC
    GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA
    AGGTTTAATG CCATTCCTCC TCACTCTGCC AGAAAGGATA AAGACATTGG AAATAAAAAG
    GAAAATTCGA GTCAGGAGGG AAATGTCCAC TCTAAGCCTC CACATACATT CACTAACGGC
    TGCCGTGGGG AACCAGAAGC TAATGCTAAA GCTAATGGCT CAATAAAAGT CGCAGATATA
    ATGGACAACA GGGATAAAAA ATGTGAGATG CCTTGCACGC CAAAAATGTC AGGCTGTAAG
    TCTGTAATGG ACAAATACAA CAGCAAAGAA GCTTTCAGAA GCCTCCAGAC AGATCCGACT
    GTAAATACAG AGCAATCAGT CTGTGCTCAA AGTCCTCAGT TGTTCAGGAT CAGCTGTAGT
    AAATCCGGGA AGCCCCCTGA CTGTGTGCTG ATGATCAGAC CTGATGGAGA GACTGAACTG
    ACAGAGGTCA AAGAAAACCC TGACGGGACT TACTCTGTCA AATACTGTCC CACTAATGAA
    GGTCTTCATT CCTTGATGGT CAAATATGTT GATGATGATT CATTTTACAA TCCTTTGAGG
    TTTCATGTCC TTCCTCACGC TGATAGTCAT GACAAACATG TGATTAATAC AACTGAAGCT
    TGTAGCAGAC AATTAGAGGA ACGTGTCAGC GCTCAAATTC CTCAGACTTT CACAACTTGC
    TGCAACACTA ACGAGGCCTC AGGGACCGAG ATGTTGAAAG CTGATGATCA TGTTGAAGTG
    TTAAATAATG AGAACACAAC CAGTGAAACA ACACACACAC CTGAACCCTC AGCGGTCAGA
    AATGCCACAC TGGAGGATTT TCAAAGGCAC CAGCCACCAT CCTCTCACAC TTCAAGTGGG
    TACAGGGGTT TTCAAAACAA GGTTGAGGGC AGGAATGCAA TCCCTCCTGC TGCCATTAAT
    CAAGCAGGGT TTATATCCTG TGATATAGTC CTGCCAGGAG ACTTAAGTGA AGGTGAAATT
    ACAGGAGAGG TGATCACGCC GTCTGGAAAG AGAGCACAGC CTGACATGAT TGATAATAAA
    GATGGGACTG TCGTTATGAA GTTTGATCCT TCAGAGGAGG GTCTTCATCA AATCCTTATT
    AAATCAGCAG AAAATGATAT ACCAGAACTT CCACTGCAAT ATTATGCAAA TAGTTTGGCT
    AATCGCAGCC CTGTGGCCTA TGGGCGTGGC CTTGTGTGTG GAATTGCAAA TGAGACTGCA
    ACATTCACCA TCTGTCAGGA GGATTCAGCC TCGAGTGAAC TGGACATCAC AATTGAGGGT
    CCGTCTGAGG CTGATGTGCA CTGCTTGGAC AATGAAGATG GCACTTGTTC TGTGAGTTAC
    CTTCCAACTG AGCCTGGAGA TTATGAGATA CAAGTTCAAC ATAATGACGT GCCTATTCCA
    GGCAGTCCTT TCAAGGCCAA AATTACAGAT GGACATATGA GAAAGTCAGA GGTCAAACTG
    GGCAGTGCGG TCGATTTTAC CCTGGACATC ACAGAAGAAG ACATTAGCCA GCTCTCCGCT
    AGCATTATGT CGCCCACAGG CAATGATGTA CCCTGCTTAC TGAAAACACA AGCTGACAGC
    CATCTCGGCG TCTCCTTCAT CCCCAGAGAG GCTGGTGAGC ATCTGGTTAG CATCATGAAG
    GATGGGGAGC ATGTGAGCAA CAGTCCCATC TCTCTGTCCA TCAGTCAGGC AGAGATTGGA
    GATGCCAGTA AGGTGAAGGC TTTCGGTCCA GGCTTACACA CCGGGCACAC CTTCTGCAAG
    TCTGAATTTG TGGTGGACAC TTGGGATGCA GGATATGGTG GGTTAACAGT TGTAATTGAA
    GGTCCCAGTA AAGTAGACGT CCACACAGAA GAGCTGGAGA ATGATGGAAC TTGCAATATA
    TCATACTGCC CAATCAAGCC TGGCAGCTAT AAAATCTCCA TCAAATTCTC AGATGAGCAC
    ATTCCAGGGA GTCCGTTTAC AGCTGTGGTG AAAGACCAAG GGCTTATGAG AGAGAACATC
    ACATACAACC AGAAAGCTGC TCCTACTGCC AGCATTGGGA GTGAATGCAG TTTAGCCTTC
    AAACTCCCAG ATGTTGATCC TGGGACTATG TCTGCTCATG TATATGAACC ATCAGGAAGT
    TTTGGAGAGG CTGTTATCCT TCCTAGAACA GGCCGTGACA TCTATGTAGT GAGCTTTCTA
    GCTAAAGAGA TGGGAGTTTA TAATGTGAGC ATACAGAACA AAGGCCAGAC GATTTCCGGC
    TGCCCACTTC AATACACCGT CGGCCCGCTG GGACGAGGAG GATCTGAGAA GGTGCAGGTT
    TGGGGACAAA GCCTTCAGAC AGCTCTGGCA ACTGTTCCAG CTGACTTTAA TATTTGGTCG
    CGTGAGGCTG GAGCAGGGGC TCTGTCTGTC TCCGTAGAGG GTCCTGGAGA GATTGAGCTC
    CACTTTGATG AGCAGATGGA CGGGTCTTGC ATTGTGACCT ATACTGCACA ACAGCCTGGT
    GACTATGAGG TTTCAGTCAT GTTTAATGAG GATCATGTTT CCCAAAGCCC TTTTCTTGTG
    TCTGTCATTG CCGCCACAGA CACTGCTGTC TCTGATCAAA CTCAGCTGTG TAACGGTCAT
    GGAGAAGCCT CTCTGTCAGG ACCGCAGATC AACACAACAT CTGACAGCAG TGCTGAGCCA
    GTTTTGCTGT CTGACGCTAG TAAAGTCATC TGCCACGGCC CAGGCCTGAG TAAGGGCTTT
    CTGGGACGCA AAAACACTTT TTATGTGGAC TGCAGTAAAG CTGGACGAAA CCTTCTGTTC
    GTGGGCATGC ATGGCCCTAC AGTTCCCTGT GAGCGGGTGT CCATCATTCA CATGGGCCAG
    TGTCAGTACA GGATCAACTA CGCTGTAAAG GAAAGAGGAA AATATGTCTT AGCAGTGAAG
    TGGGGAGATG AGCACATTCC TGGATCGCCG TTTTACATTA GTGTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330211.1
    CDS996..7706
    Translation

    Target ORF information:

    RefSeq Version XM_021474536.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG
    CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT
    CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA
    AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT
    GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT
    GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT
    TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC
    AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG
    AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA
    AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA
    GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT
    GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT
    GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA
    GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG
    GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA
    GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT
    GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA
    TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT
    GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT
    GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA
    GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC
    AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT
    CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA
    ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT
    GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT
    TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC
    AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT
    AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT
    ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC
    ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA
    CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC
    AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT
    CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT
    GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC
    AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC
    GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA
    AGGTTTAATG CCATTCCTCC TCACTCTGCC AGAAAGGATA AAGACATTGG AAATAAAAAG
    GAAAATTCGA GTCAGGAGGG AAATGTCCAC TCTAAGCCTC CACATACATT CACTAACGGC
    TGCCGTGGGG AACCAGAAGC TAATGCTAAA GCTAATGGCT CAATAAAAGT CGCAGATATA
    ATGGACAACA GGGATAAAAA ATGTGAGATG CCTTGCACGC CAAAAATGTC AGGCTGTAAG
    TCTGTAATGG ACAAATACAA CAGCAAAGAA GCTTTCAGAA GCCTCCAGAC AGATCCGACT
    GTAAATACAG AGCAATCAGT CTGTGCTCAA AGTCCTCAGT TGTTCAGGAT CAGCTGTAGT
    AAATCCGGGA AGCCCCCTGA CTGTGTGCTG ATGATCAGAC CTGATGGAGA GACTGAACTG
    ACAGAGGTCA AAGAAAACCC TGACGGGACT TACTCTGTCA AATACTGTCC CACTAATGAA
    GGTCTTCATT CCTTGATGGT CAAATATGTT GATGATGATT CATTTTACAA TCCTTTGAGG
    TTTCATGTCC TTCCTCACGC TGATAGTCAT GACAAACATG TGATTAATAC AACTGAAGCT
    TGTAGCAGAC AATTAGAGGA ACGTGTCAGC GCTCAAATTC CTCAGACTTT CACAACTTGC
    TGCAACACTA ACGAGGCCTC AGGGACCGAG ATGTTGAAAG CTGATGATCA TGTTGAAGTG
    TTAAATAATG AGAACACAAC CAGTGAAACA ACACACACAC CTGAACCCTC AGCGGTCAGA
    AATGCCACAC TGGAGGATTT TCAAAGGCAC CAGCCACCAT CCTCTCACAC TTCAAGTGGG
    TACAGGGGTT TTCAAAACAA GGTTGAGGGC AGGAATGCAA TCCCTCCTGC TGCCATTAAT
    CAAGCAGGGT TTATATCCTG TGATATAGTC CTGCCAGGAG ACTTAAGTGA AGGTGAAATT
    ACAGGAGAGG TGATCACGCC GTCTGGAAAG AGAGCACAGC CTGACATGAT TGATAATAAA
    GATGGGACTG TCGTTATGAA GTTTGATCCT TCAGAGGAGG GTCTTCATCA AATCCTTATT
    AAATCAGCAG AAAATGATAT ACCAGAACTT CCACTGCAAT ATTATGCAAA TAGTTTGGCT
    AATCGCAGCC CTGTGGCCTA TGGGCGTGGC CTTGTGTGTG GAATTGCAAA TGAGACTGCA
    ACATTCACCA TCTGTCAGGA GGATTCAGCC TCGAGTGAAC TGGACATCAC AATTGAGGGT
    CCGTCTGAGG CTGATGTGCA CTGCTTGGAC AATGAAGATG GCACTTGTTC TGTGAGTTAC
    CTTCCAACTG AGCCTGGAGA TTATGAGATA CAAGTTCAAC ATAATGACGT GCCTATTCCA
    GGCAGTCCTT TCAAGGCCAA AATTACAGAT GGACATATGA GAAAGTCAGA GGTCAAACTG
    GGCAGTGCGG TCGATTTTAC CCTGGACATC ACAGAAGAAG ACATTAGCCA GCTCTCCGCT
    AGCATTATGT CGCCCACAGG CAATGATGTA CCCTGCTTAC TGAAAACACA AGCTGACAGC
    CATCTCGGCG TCTCCTTCAT CCCCAGAGAG GCTGGTGAGC ATCTGGTTAG CATCATGAAG
    GATGGGGAGC ATGTGAGCAA CAGTCCCATC TCTCTGTCCA TCAGTCAGGC AGAGATTGGA
    GATGCCAGTA AGGTGAAGGC TTTCGGTCCA GGCTTACACA CCGGGCACAC CTTCTGCAAG
    TCTGAATTTG TGGTGGACAC TTGGGATGCA GGATATGGTG GGTTAACAGT TGTAATTGAA
    GGTCCCAGTA AAGTAGACGT CCACACAGAA GAGCTGGAGA ATGATGGAAC TTGCAATATA
    TCATACTGCC CAATCAAGCC TGGCAGCTAT AAAATCTCCA TCAAATTCTC AGATGAGCAC
    ATTCCAGGGA GTCCGTTTAC AGCTGTGGTG AAAGACCAAG GGCTTATGAG AGAGAACATC
    ACATACAACC AGAAAGCTGC TCCTACTGCC AGCATTGGGA GTGAATGCAG TTTAGCCTTC
    AAACTCCCAG ATGTTGATCC TGGGACTATG TCTGCTCATG TATATGAACC ATCAGGAAGT
    TTTGGAGAGG CTGTTATCCT TCCTAGAACA GGCCGTGACA TCTATGTAGT GAGCTTTCTA
    GCTAAAGAGA TGGGAGTTTA TAATGTGAGC ATACAGAACA AAGGCCAGAC GATTTCCGGC
    TGCCCACTTC AATACACCGT CGGCCCGCTG GGACGAGGAG GATCTGAGAA GGTGCAGGTT
    TGGGGACAAA GCCTTCAGAC AGCTCTGGCA ACTGTTCCAG CTGACTTTAA TATTTGGTCG
    CGTGAGGCTG GAGCAGGGGC TCTGTCTGTC TCCGTAGAGG GTCCTGGAGA GATTGAGCTC
    CACTTTGATG AGCAGATGGA CGGGTCTTGC ATTGTGACCT ATACTGCACA ACAGCCTGGT
    GACTATGAGG TTTCAGTCAT GTTTAATGAG GATCATGTTT CCCAAAGCCC TTTTCTTGTG
    TCTGTCATTG CCGCCACAGA CACTGCTGTC TCTGATCAAA CTCAGCTGTG TAACGGTCAT
    GGAGAAGCCT CTCTGTCAGG ACCGCAGATC AACACAACAT CTGACAGCAG TGCTGAGCCA
    GTTTTGCTGT CTGACGCTAG TAAAGTCATC TGCCACGGCC CAGGCCTGAG TAAGGGCTTT
    CTGGGACGCA AAAACACTTT TTATGTGGAC TGCAGTAAAG CTGGACGAAA CCTTCTGTTC
    GTGGGCATGC ATGGCCCTAC AGTTCCCTGT GAGCGGGTGT CCATCATTCA CATGGGCCAG
    TGTCAGTACA GGATCAACTA CGCTGTAAAG GAAAGAGGAA AATATGTCTT AGCAGTGAAG
    TGGGGAGATG AGCACATTCC TGGATCGCCG TTTTACATTA GTGTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146813
    Clone ID Related Accession (Same CDS sequence) XM_021474537.1
    Accession Version XM_021474537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6681bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filamin-B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG
    CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT
    CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA
    AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT
    GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT
    GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT
    TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC
    AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG
    AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA
    AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA
    GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT
    GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT
    GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA
    GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG
    GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA
    GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT
    GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA
    TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT
    GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT
    GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA
    GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC
    AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT
    CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA
    ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT
    GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT
    TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC
    AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT
    AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT
    ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC
    ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA
    CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC
    AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT
    CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT
    GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC
    AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC
    GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA
    AGAAAGGATA AAGACATTGG AAATAAAAAG GAAAATTCGA GTCAGGAGGG AAATGTCCAC
    TCTAAGCCTC CACATACATT CACTAACGGC TGCCGTGGGG AACCAGAAGC TAATGCTAAA
    GCTAATGGCT CAATAAAAGT CGCAGATATA ATGGACAACA GGGATAAAAA ATGTGAGATG
    CCTTGCACGC CAAAAATGTC AGGCTGTAAG TCTGTAATGG ACAAATACAA CAGCAAAGAA
    GCTTTCAGAA GCCTCCAGAC AGATCCGACT GTAAATACAG AGCAATCAGT CTGTGCTCAA
    AGTCCTCAGT TGTTCAGGAT CAGCTGTAGT AAATCCGGGA AGCCCCCTGA CTGTGTGCTG
    ATGATCAGAC CTGATGGAGA GACTGAACTG ACAGAGGTCA AAGAAAACCC TGACGGGACT
    TACTCTGTCA AATACTGTCC CACTAATGAA GGTCTTCATT CCTTGATGGT CAAATATGTT
    GATGATGATT CATTTTACAA TCCTTTGAGG TTTCATGTCC TTCCTCACGC TGATAGTCAT
    GACAAACATG TGATTAATAC AACTGAAGCT TGTAGCAGAC AATTAGAGGA ACGTGTCAGC
    GCTCAAATTC CTCAGACTTT CACAACTTGC TGCAACACTA ACGAGGCCTC AGGGACCGAG
    ATGTTGAAAG CTGATGATCA TGTTGAAGTG TTAAATAATG AGAACACAAC CAGTGAAACA
    ACACACACAC CTGAACCCTC AGCGGTCAGA AATGCCACAC TGGAGGATTT TCAAAGGCAC
    CAGCCACCAT CCTCTCACAC TTCAAGTGGG TACAGGGGTT TTCAAAACAA GGTTGAGGGC
    AGGAATGCAA TCCCTCCTGC TGCCATTAAT CAAGCAGGGT TTATATCCTG TGATATAGTC
    CTGCCAGGAG ACTTAAGTGA AGGTGAAATT ACAGGAGAGG TGATCACGCC GTCTGGAAAG
    AGAGCACAGC CTGACATGAT TGATAATAAA GATGGGACTG TCGTTATGAA GTTTGATCCT
    TCAGAGGAGG GTCTTCATCA AATCCTTATT AAATCAGCAG AAAATGATAT ACCAGAACTT
    CCACTGCAAT ATTATGCAAA TAGTTTGGCT AATCGCAGCC CTGTGGCCTA TGGGCGTGGC
    CTTGTGTGTG GAATTGCAAA TGAGACTGCA ACATTCACCA TCTGTCAGGA GGATTCAGCC
    TCGAGTGAAC TGGACATCAC AATTGAGGGT CCGTCTGAGG CTGATGTGCA CTGCTTGGAC
    AATGAAGATG GCACTTGTTC TGTGAGTTAC CTTCCAACTG AGCCTGGAGA TTATGAGATA
    CAAGTTCAAC ATAATGACGT GCCTATTCCA GGCAGTCCTT TCAAGGCCAA AATTACAGAT
    GGACATATGA GAAAGTCAGA GGTCAAACTG GGCAGTGCGG TCGATTTTAC CCTGGACATC
    ACAGAAGAAG ACATTAGCCA GCTCTCCGCT AGCATTATGT CGCCCACAGG CAATGATGTA
    CCCTGCTTAC TGAAAACACA AGCTGACAGC CATCTCGGCG TCTCCTTCAT CCCCAGAGAG
    GCTGGTGAGC ATCTGGTTAG CATCATGAAG GATGGGGAGC ATGTGAGCAA CAGTCCCATC
    TCTCTGTCCA TCAGTCAGGC AGAGATTGGA GATGCCAGTA AGGTGAAGGC TTTCGGTCCA
    GGCTTACACA CCGGGCACAC CTTCTGCAAG TCTGAATTTG TGGTGGACAC TTGGGATGCA
    GGATATGGTG GGTTAACAGT TGTAATTGAA GGTCCCAGTA AAGTAGACGT CCACACAGAA
    GAGCTGGAGA ATGATGGAAC TTGCAATATA TCATACTGCC CAATCAAGCC TGGCAGCTAT
    AAAATCTCCA TCAAATTCTC AGATGAGCAC ATTCCAGGGA GTCCGTTTAC AGCTGTGGTG
    AAAGACCAAG GGCTTATGAG AGAGAACATC ACATACAACC AGAAAGCTGC TCCTACTGCC
    AGCATTGGGA GTGAATGCAG TTTAGCCTTC AAACTCCCAG ATGTTGATCC TGGGACTATG
    TCTGCTCATG TATATGAACC ATCAGGAAGT TTTGGAGAGG CTGTTATCCT TCCTAGAACA
    GGCCGTGACA TCTATGTAGT GAGCTTTCTA GCTAAAGAGA TGGGAGTTTA TAATGTGAGC
    ATACAGAACA AAGGCCAGAC GATTTCCGGC TGCCCACTTC AATACACCGT CGGCCCGCTG
    GGACGAGGAG GATCTGAGAA GGTGCAGGTT TGGGGACAAA GCCTTCAGAC AGCTCTGGCA
    ACTGTTCCAG CTGACTTTAA TATTTGGTCG CGTGAGGCTG GAGCAGGGGC TCTGTCTGTC
    TCCGTAGAGG GTCCTGGAGA GATTGAGCTC CACTTTGATG AGCAGATGGA CGGGTCTTGC
    ATTGTGACCT ATACTGCACA ACAGCCTGGT GACTATGAGG TTTCAGTCAT GTTTAATGAG
    GATCATGTTT CCCAAAGCCC TTTTCTTGTG TCTGTCATTG CCGCCACAGA CACTGCTGTC
    TCTGATCAAA CTCAGCTGTG TAACGGTCAT GGAGAAGCCT CTCTGTCAGG ACCGCAGATC
    AACACAACAT CTGACAGCAG TGCTGAGCCA GTTTTGCTGT CTGACGCTAG TAAAGTCATC
    TGCCACGGCC CAGGCCTGAG TAAGGGCTTT CTGGGACGCA AAAACACTTT TTATGTGGAC
    TGCAGTAAAG CTGGACGAAA CCTTCTGTTC GTGGGCATGC ATGGCCCTAC AGTTCCCTGT
    GAGCGGGTGT CCATCATTCA CATGGGCCAG TGTCAGTACA GGATCAACTA CGCTGTAAAG
    GAAAGAGGAA AATATGTCTT AGCAGTGAAG TGGGGAGATG AGCACATTCC TGGATCGCCG
    TTTTACATTA GTGTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330212.1
    CDS996..7676
    Translation

    Target ORF information:

    RefSeq Version XM_021474537.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG
    CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT
    CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA
    AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT
    GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT
    GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT
    TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC
    AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG
    AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA
    AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA
    GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT
    GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT
    GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA
    GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG
    GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA
    GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT
    GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA
    TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT
    GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT
    GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA
    GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC
    AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT
    CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA
    ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT
    GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT
    TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC
    AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT
    AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT
    ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC
    ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA
    CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC
    AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT
    CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT
    GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC
    AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC
    GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA
    AGAAAGGATA AAGACATTGG AAATAAAAAG GAAAATTCGA GTCAGGAGGG AAATGTCCAC
    TCTAAGCCTC CACATACATT CACTAACGGC TGCCGTGGGG AACCAGAAGC TAATGCTAAA
    GCTAATGGCT CAATAAAAGT CGCAGATATA ATGGACAACA GGGATAAAAA ATGTGAGATG
    CCTTGCACGC CAAAAATGTC AGGCTGTAAG TCTGTAATGG ACAAATACAA CAGCAAAGAA
    GCTTTCAGAA GCCTCCAGAC AGATCCGACT GTAAATACAG AGCAATCAGT CTGTGCTCAA
    AGTCCTCAGT TGTTCAGGAT CAGCTGTAGT AAATCCGGGA AGCCCCCTGA CTGTGTGCTG
    ATGATCAGAC CTGATGGAGA GACTGAACTG ACAGAGGTCA AAGAAAACCC TGACGGGACT
    TACTCTGTCA AATACTGTCC CACTAATGAA GGTCTTCATT CCTTGATGGT CAAATATGTT
    GATGATGATT CATTTTACAA TCCTTTGAGG TTTCATGTCC TTCCTCACGC TGATAGTCAT
    GACAAACATG TGATTAATAC AACTGAAGCT TGTAGCAGAC AATTAGAGGA ACGTGTCAGC
    GCTCAAATTC CTCAGACTTT CACAACTTGC TGCAACACTA ACGAGGCCTC AGGGACCGAG
    ATGTTGAAAG CTGATGATCA TGTTGAAGTG TTAAATAATG AGAACACAAC CAGTGAAACA
    ACACACACAC CTGAACCCTC AGCGGTCAGA AATGCCACAC TGGAGGATTT TCAAAGGCAC
    CAGCCACCAT CCTCTCACAC TTCAAGTGGG TACAGGGGTT TTCAAAACAA GGTTGAGGGC
    AGGAATGCAA TCCCTCCTGC TGCCATTAAT CAAGCAGGGT TTATATCCTG TGATATAGTC
    CTGCCAGGAG ACTTAAGTGA AGGTGAAATT ACAGGAGAGG TGATCACGCC GTCTGGAAAG
    AGAGCACAGC CTGACATGAT TGATAATAAA GATGGGACTG TCGTTATGAA GTTTGATCCT
    TCAGAGGAGG GTCTTCATCA AATCCTTATT AAATCAGCAG AAAATGATAT ACCAGAACTT
    CCACTGCAAT ATTATGCAAA TAGTTTGGCT AATCGCAGCC CTGTGGCCTA TGGGCGTGGC
    CTTGTGTGTG GAATTGCAAA TGAGACTGCA ACATTCACCA TCTGTCAGGA GGATTCAGCC
    TCGAGTGAAC TGGACATCAC AATTGAGGGT CCGTCTGAGG CTGATGTGCA CTGCTTGGAC
    AATGAAGATG GCACTTGTTC TGTGAGTTAC CTTCCAACTG AGCCTGGAGA TTATGAGATA
    CAAGTTCAAC ATAATGACGT GCCTATTCCA GGCAGTCCTT TCAAGGCCAA AATTACAGAT
    GGACATATGA GAAAGTCAGA GGTCAAACTG GGCAGTGCGG TCGATTTTAC CCTGGACATC
    ACAGAAGAAG ACATTAGCCA GCTCTCCGCT AGCATTATGT CGCCCACAGG CAATGATGTA
    CCCTGCTTAC TGAAAACACA AGCTGACAGC CATCTCGGCG TCTCCTTCAT CCCCAGAGAG
    GCTGGTGAGC ATCTGGTTAG CATCATGAAG GATGGGGAGC ATGTGAGCAA CAGTCCCATC
    TCTCTGTCCA TCAGTCAGGC AGAGATTGGA GATGCCAGTA AGGTGAAGGC TTTCGGTCCA
    GGCTTACACA CCGGGCACAC CTTCTGCAAG TCTGAATTTG TGGTGGACAC TTGGGATGCA
    GGATATGGTG GGTTAACAGT TGTAATTGAA GGTCCCAGTA AAGTAGACGT CCACACAGAA
    GAGCTGGAGA ATGATGGAAC TTGCAATATA TCATACTGCC CAATCAAGCC TGGCAGCTAT
    AAAATCTCCA TCAAATTCTC AGATGAGCAC ATTCCAGGGA GTCCGTTTAC AGCTGTGGTG
    AAAGACCAAG GGCTTATGAG AGAGAACATC ACATACAACC AGAAAGCTGC TCCTACTGCC
    AGCATTGGGA GTGAATGCAG TTTAGCCTTC AAACTCCCAG ATGTTGATCC TGGGACTATG
    TCTGCTCATG TATATGAACC ATCAGGAAGT TTTGGAGAGG CTGTTATCCT TCCTAGAACA
    GGCCGTGACA TCTATGTAGT GAGCTTTCTA GCTAAAGAGA TGGGAGTTTA TAATGTGAGC
    ATACAGAACA AAGGCCAGAC GATTTCCGGC TGCCCACTTC AATACACCGT CGGCCCGCTG
    GGACGAGGAG GATCTGAGAA GGTGCAGGTT TGGGGACAAA GCCTTCAGAC AGCTCTGGCA
    ACTGTTCCAG CTGACTTTAA TATTTGGTCG CGTGAGGCTG GAGCAGGGGC TCTGTCTGTC
    TCCGTAGAGG GTCCTGGAGA GATTGAGCTC CACTTTGATG AGCAGATGGA CGGGTCTTGC
    ATTGTGACCT ATACTGCACA ACAGCCTGGT GACTATGAGG TTTCAGTCAT GTTTAATGAG
    GATCATGTTT CCCAAAGCCC TTTTCTTGTG TCTGTCATTG CCGCCACAGA CACTGCTGTC
    TCTGATCAAA CTCAGCTGTG TAACGGTCAT GGAGAAGCCT CTCTGTCAGG ACCGCAGATC
    AACACAACAT CTGACAGCAG TGCTGAGCCA GTTTTGCTGT CTGACGCTAG TAAAGTCATC
    TGCCACGGCC CAGGCCTGAG TAAGGGCTTT CTGGGACGCA AAAACACTTT TTATGTGGAC
    TGCAGTAAAG CTGGACGAAA CCTTCTGTTC GTGGGCATGC ATGGCCCTAC AGTTCCCTGT
    GAGCGGGTGT CCATCATTCA CATGGGCCAG TGTCAGTACA GGATCAACTA CGCTGTAAAG
    GAAAGAGGAA AATATGTCTT AGCAGTGAAG TGGGGAGATG AGCACATTCC TGGATCGCCG
    TTTTACATTA GTGTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27643
    Clone ID Related Accession (Same CDS sequence) XM_686387.7 , XM_021469933.1
    Accession Version XM_686387.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product filamin-A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_686387.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG
    CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT
    GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC
    ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG
    TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC
    AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC
    CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA
    TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC
    TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG
    GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC
    GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT
    GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG
    GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC
    GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC
    ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA
    ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT
    GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG
    AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC
    TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA
    ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC
    AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA
    GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC
    GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA
    ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA
    TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA
    CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT
    AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT
    CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA
    AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG
    GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT
    TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA
    GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC
    TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT
    GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT
    TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT
    CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT
    GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG
    AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG
    GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC
    AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG
    GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA
    GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG
    AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC
    AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT
    TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC
    CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA
    CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT
    AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT
    GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA
    CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT
    TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG
    TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG
    GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT
    GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA
    GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC
    CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC
    ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG
    GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT
    GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT
    TTCAAGGCCA AAATTACAGA TGGACATATG AGAAAGTCAG AGGTCAAACT GGGCAGTGCG
    GTCGATTTTA CCCTGGACAT CACCGAAGAA GACATTAGCC AGCTCTCCGC TAGCATTATG
    TCGCCCACAG GCAATGATGT ACCCTGCTTA CTGAAAACAC AAGCTGACAG CCATCTCGGC
    ATCTCCTTCA TCCCCAGAGA GGCTGGTGAG CATCTGGTTA GCATCATGAA GGATGGGGAG
    CATGTGAGCA ACAGTCCCAT CTCTCTGTCC ATCAGTCAGG CAGAGATTGG AGATGCCAGT
    AAGGTGAAGG CCTTCGGTCC AGGCTTACAC ACCGGGCACA CCTTCTGCAA GTCTGAATTT
    GTGGTGGACA CTTGGGATGC AGGATATGGT GGCTTAACAG TTGTAATTGA AGGTCCCAGT
    AAAGTAGACG TCCACACAAA AGAGCTGGAG AATGATGGAA CTTGCAATAT ATCATACTGC
    CCAATCAAGC CTGGCAGCTA TAAAATCTCC ATCAAATTCT CAGATGAGCA CATTCCAGGG
    AGTCCGTTTA CAGCTGTGGT GAAAGACCAA GGGCTTATGA GAGAGAACAT CACATACAAC
    CAGAAAGCTG CTCCTACTGC CAGCATTGGG AGTGAATGCA GTTTAGCCTT CAAACTCCAA
    GATGTTGATC CTGGGACTAT GTCTGCTCAT GTATATGAAC CATCAGGAAG TTTTGGAGAG
    GCTGTTATCC TTCCTAGTAC AGGCCGTGAC ATCTATGTAG TGAGCTTTCT AGCTAAAGAG
    ATGGGAGTTT ATAATGTGAG CATACAGAAC AAAGGCCAGA CGATTTCCGG CTGCCCACTT
    CAATACACCG TCGGCCCGCT GGGACGAGGA GGATCTGAGA AGGTGCAGGT TTGGGGACAA
    AGCCTTCAGA CGGCTCTGGC AACTGTTCCA GCTGACTTTA ATATTTGGTC GCGTGAGGCT
    GGAGCAGGGG CTCTTTCTGT CTCCGTAGAG GGTCCTGGAG AGATTGAGCT CCTCTTTGAT
    GAGCAGATGG ACGGGTCTTG CATTGTGACC TATACTGCAC AACAGCCTGG TGACTATGAG
    GTTTCAGTCA TGTTTGATGA GGATCATGTT TCCCAAAGCC CTTTTCTTGT GTCTGTCATT
    GCCGCCACAG ACACTGCTGT CTCTGATCAA ACTCAGCTGT GTAACGGTCA TGGAGAAGCC
    TCTCTGTCAG GACCGCAGAT CAACACAACA TCTGACAGCA GTGCTGAGCC AGTTTTGCTG
    TCTGACGCTA GTAAAGTCAT CTGCCACGGC CCAGGCCTGA GTAAGGGCTT TCTGGGACGC
    AAAAACACTT TTTATGTGGA CTGCAGTAAA GCTGGACGAA ACCTTCTGTT CGTGGGCATG
    CATGGCCCTA CAGTTCCCTG TGAGCGGGTG TCCATCATTC ACATGGGCCA GTGTCAGTAC
    AGGATCAACT ACGCTGTAAA GGAAAGAGGA AAATATGTCT TAGCAGTGAA GTGGGGAGAT
    GAGCACATTC CTGGATCGCC GTTTTATATT AGTGTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_691479.5
    CDS344..7045
    Translation

    Target ORF information:

    RefSeq Version XM_686387.7
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio filamin B, beta (actin binding protein 278) (flnb), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_686387.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG
    CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT
    GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC
    ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG
    TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC
    AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC
    CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA
    TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC
    TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG
    GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC
    GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT
    GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG
    GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC
    GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC
    ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA
    ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT
    GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG
    AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC
    TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA
    ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC
    AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA
    GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC
    GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA
    ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA
    TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA
    CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT
    AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT
    CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA
    AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG
    GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT
    TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA
    GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC
    TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT
    GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT
    TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT
    CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT
    GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG
    AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG
    GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC
    AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG
    GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA
    GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG
    AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC
    AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT
    TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC
    CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA
    CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT
    AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT
    GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA
    CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT
    TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG
    TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG
    GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT
    GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA
    GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC
    CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC
    ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG
    GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT
    GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT
    TTCAAGGCCA AAATTACAGA TGGACATATG AGAAAGTCAG AGGTCAAACT GGGCAGTGCG
    GTCGATTTTA CCCTGGACAT CACCGAAGAA GACATTAGCC AGCTCTCCGC TAGCATTATG
    TCGCCCACAG GCAATGATGT ACCCTGCTTA CTGAAAACAC AAGCTGACAG CCATCTCGGC
    ATCTCCTTCA TCCCCAGAGA GGCTGGTGAG CATCTGGTTA GCATCATGAA GGATGGGGAG
    CATGTGAGCA ACAGTCCCAT CTCTCTGTCC ATCAGTCAGG CAGAGATTGG AGATGCCAGT
    AAGGTGAAGG CCTTCGGTCC AGGCTTACAC ACCGGGCACA CCTTCTGCAA GTCTGAATTT
    GTGGTGGACA CTTGGGATGC AGGATATGGT GGCTTAACAG TTGTAATTGA AGGTCCCAGT
    AAAGTAGACG TCCACACAAA AGAGCTGGAG AATGATGGAA CTTGCAATAT ATCATACTGC
    CCAATCAAGC CTGGCAGCTA TAAAATCTCC ATCAAATTCT CAGATGAGCA CATTCCAGGG
    AGTCCGTTTA CAGCTGTGGT GAAAGACCAA GGGCTTATGA GAGAGAACAT CACATACAAC
    CAGAAAGCTG CTCCTACTGC CAGCATTGGG AGTGAATGCA GTTTAGCCTT CAAACTCCAA
    GATGTTGATC CTGGGACTAT GTCTGCTCAT GTATATGAAC CATCAGGAAG TTTTGGAGAG
    GCTGTTATCC TTCCTAGTAC AGGCCGTGAC ATCTATGTAG TGAGCTTTCT AGCTAAAGAG
    ATGGGAGTTT ATAATGTGAG CATACAGAAC AAAGGCCAGA CGATTTCCGG CTGCCCACTT
    CAATACACCG TCGGCCCGCT GGGACGAGGA GGATCTGAGA AGGTGCAGGT TTGGGGACAA
    AGCCTTCAGA CGGCTCTGGC AACTGTTCCA GCTGACTTTA ATATTTGGTC GCGTGAGGCT
    GGAGCAGGGG CTCTTTCTGT CTCCGTAGAG GGTCCTGGAG AGATTGAGCT CCTCTTTGAT
    GAGCAGATGG ACGGGTCTTG CATTGTGACC TATACTGCAC AACAGCCTGG TGACTATGAG
    GTTTCAGTCA TGTTTGATGA GGATCATGTT TCCCAAAGCC CTTTTCTTGT GTCTGTCATT
    GCCGCCACAG ACACTGCTGT CTCTGATCAA ACTCAGCTGT GTAACGGTCA TGGAGAAGCC
    TCTCTGTCAG GACCGCAGAT CAACACAACA TCTGACAGCA GTGCTGAGCC AGTTTTGCTG
    TCTGACGCTA GTAAAGTCAT CTGCCACGGC CCAGGCCTGA GTAAGGGCTT TCTGGGACGC
    AAAAACACTT TTTATGTGGA CTGCAGTAAA GCTGGACGAA ACCTTCTGTT CGTGGGCATG
    CATGGCCCTA CAGTTCCCTG TGAGCGGGTG TCCATCATTC ACATGGGCCA GTGTCAGTAC
    AGGATCAACT ACGCTGTAAA GGAAAGAGGA AAATATGTCT TAGCAGTGAA GTGGGGAGAT
    GAGCACATTC CTGGATCGCC GTTTTATATT AGTGTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27643
    Clone ID Related Accession (Same CDS sequence) XM_686387.7 , XM_021469933.1
    Accession Version XM_021469933.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filamin-A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG
    CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT
    GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC
    ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG
    TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC
    AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC
    CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA
    TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC
    TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG
    GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC
    GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT
    GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG
    GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC
    GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC
    ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA
    ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT
    GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG
    AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC
    TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA
    ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC
    AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA
    GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC
    GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA
    ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA
    TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA
    CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT
    AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT
    CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA
    AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG
    GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT
    TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA
    GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC
    TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT
    GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT
    TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT
    CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT
    GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG
    AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG
    GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC
    AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG
    GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA
    GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG
    AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC
    AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT
    TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC
    CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA
    CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT
    AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT
    GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA
    CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT
    TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG
    TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG
    GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT
    GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA
    GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC
    CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC
    ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG
    GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT
    GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT
    TTCAAGGCCA AAATTACAGA TGGACATATG AGAAAGTCAG AGGTCAAACT GGGCAGTGCG
    GTCGATTTTA CCCTGGACAT CACCGAAGAA GACATTAGCC AGCTCTCCGC TAGCATTATG
    TCGCCCACAG GCAATGATGT ACCCTGCTTA CTGAAAACAC AAGCTGACAG CCATCTCGGC
    ATCTCCTTCA TCCCCAGAGA GGCTGGTGAG CATCTGGTTA GCATCATGAA GGATGGGGAG
    CATGTGAGCA ACAGTCCCAT CTCTCTGTCC ATCAGTCAGG CAGAGATTGG AGATGCCAGT
    AAGGTGAAGG CCTTCGGTCC AGGCTTACAC ACCGGGCACA CCTTCTGCAA GTCTGAATTT
    GTGGTGGACA CTTGGGATGC AGGATATGGT GGCTTAACAG TTGTAATTGA AGGTCCCAGT
    AAAGTAGACG TCCACACAAA AGAGCTGGAG AATGATGGAA CTTGCAATAT ATCATACTGC
    CCAATCAAGC CTGGCAGCTA TAAAATCTCC ATCAAATTCT CAGATGAGCA CATTCCAGGG
    AGTCCGTTTA CAGCTGTGGT GAAAGACCAA GGGCTTATGA GAGAGAACAT CACATACAAC
    CAGAAAGCTG CTCCTACTGC CAGCATTGGG AGTGAATGCA GTTTAGCCTT CAAACTCCAA
    GATGTTGATC CTGGGACTAT GTCTGCTCAT GTATATGAAC CATCAGGAAG TTTTGGAGAG
    GCTGTTATCC TTCCTAGTAC AGGCCGTGAC ATCTATGTAG TGAGCTTTCT AGCTAAAGAG
    ATGGGAGTTT ATAATGTGAG CATACAGAAC AAAGGCCAGA CGATTTCCGG CTGCCCACTT
    CAATACACCG TCGGCCCGCT GGGACGAGGA GGATCTGAGA AGGTGCAGGT TTGGGGACAA
    AGCCTTCAGA CGGCTCTGGC AACTGTTCCA GCTGACTTTA ATATTTGGTC GCGTGAGGCT
    GGAGCAGGGG CTCTTTCTGT CTCCGTAGAG GGTCCTGGAG AGATTGAGCT CCTCTTTGAT
    GAGCAGATGG ACGGGTCTTG CATTGTGACC TATACTGCAC AACAGCCTGG TGACTATGAG
    GTTTCAGTCA TGTTTGATGA GGATCATGTT TCCCAAAGCC CTTTTCTTGT GTCTGTCATT
    GCCGCCACAG ACACTGCTGT CTCTGATCAA ACTCAGCTGT GTAACGGTCA TGGAGAAGCC
    TCTCTGTCAG GACCGCAGAT CAACACAACA TCTGACAGCA GTGCTGAGCC AGTTTTGCTG
    TCTGACGCTA GTAAAGTCAT CTGCCACGGC CCAGGCCTGA GTAAGGGCTT TCTGGGACGC
    AAAAACACTT TTTATGTGGA CTGCAGTAAA GCTGGACGAA ACCTTCTGTT CGTGGGCATG
    CATGGCCCTA CAGTTCCCTG TGAGCGGGTG TCCATCATTC ACATGGGCCA GTGTCAGTAC
    AGGATCAACT ACGCTGTAAA GGAAAGAGGA AAATATGTCT TAGCAGTGAA GTGGGGAGAT
    GAGCACATTC CTGGATCGCC GTTTTATATT AGTGTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325608.1
    CDS559..7260
    Translation

    Target ORF information:

    RefSeq Version XM_021469933.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
    TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG
    AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG
    CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG
    CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG
    CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG
    GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC
    GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT
    CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA
    TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT
    CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG
    CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT
    CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG
    CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA
    GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC
    CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT
    GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG
    GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA
    CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG
    TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA
    TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG
    CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC
    AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA
    GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC
    GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA
    GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG
    CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC
    AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT
    AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT
    GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC
    ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG
    TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC
    AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC
    CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA
    TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC
    TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG
    GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC
    GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT
    GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG
    GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC
    GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC
    ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA
    ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT
    GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG
    AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC
    TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA
    ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC
    AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA
    GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC
    GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA
    ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA
    TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA
    CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT
    AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT
    CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA
    AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG
    GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT
    TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA
    GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC
    TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT
    GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT
    TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT
    CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT
    GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG
    AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG
    GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC
    AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG
    GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA
    GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG
    AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC
    AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT
    TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC
    CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA
    CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT
    AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT
    GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA
    CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT
    TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG
    TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG
    GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT
    GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA
    GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC
    CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC
    ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG
    GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT
    GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT
    TTCAAGGCCA AAATTACAGA TGGACATATG AGAAAGTCAG AGGTCAAACT GGGCAGTGCG
    GTCGATTTTA CCCTGGACAT CACCGAAGAA GACATTAGCC AGCTCTCCGC TAGCATTATG
    TCGCCCACAG GCAATGATGT ACCCTGCTTA CTGAAAACAC AAGCTGACAG CCATCTCGGC
    ATCTCCTTCA TCCCCAGAGA GGCTGGTGAG CATCTGGTTA GCATCATGAA GGATGGGGAG
    CATGTGAGCA ACAGTCCCAT CTCTCTGTCC ATCAGTCAGG CAGAGATTGG AGATGCCAGT
    AAGGTGAAGG CCTTCGGTCC AGGCTTACAC ACCGGGCACA CCTTCTGCAA GTCTGAATTT
    GTGGTGGACA CTTGGGATGC AGGATATGGT GGCTTAACAG TTGTAATTGA AGGTCCCAGT
    AAAGTAGACG TCCACACAAA AGAGCTGGAG AATGATGGAA CTTGCAATAT ATCATACTGC
    CCAATCAAGC CTGGCAGCTA TAAAATCTCC ATCAAATTCT CAGATGAGCA CATTCCAGGG
    AGTCCGTTTA CAGCTGTGGT GAAAGACCAA GGGCTTATGA GAGAGAACAT CACATACAAC
    CAGAAAGCTG CTCCTACTGC CAGCATTGGG AGTGAATGCA GTTTAGCCTT CAAACTCCAA
    GATGTTGATC CTGGGACTAT GTCTGCTCAT GTATATGAAC CATCAGGAAG TTTTGGAGAG
    GCTGTTATCC TTCCTAGTAC AGGCCGTGAC ATCTATGTAG TGAGCTTTCT AGCTAAAGAG
    ATGGGAGTTT ATAATGTGAG CATACAGAAC AAAGGCCAGA CGATTTCCGG CTGCCCACTT
    CAATACACCG TCGGCCCGCT GGGACGAGGA GGATCTGAGA AGGTGCAGGT TTGGGGACAA
    AGCCTTCAGA CGGCTCTGGC AACTGTTCCA GCTGACTTTA ATATTTGGTC GCGTGAGGCT
    GGAGCAGGGG CTCTTTCTGT CTCCGTAGAG GGTCCTGGAG AGATTGAGCT CCTCTTTGAT
    GAGCAGATGG ACGGGTCTTG CATTGTGACC TATACTGCAC AACAGCCTGG TGACTATGAG
    GTTTCAGTCA TGTTTGATGA GGATCATGTT TCCCAAAGCC CTTTTCTTGT GTCTGTCATT
    GCCGCCACAG ACACTGCTGT CTCTGATCAA ACTCAGCTGT GTAACGGTCA TGGAGAAGCC
    TCTCTGTCAG GACCGCAGAT CAACACAACA TCTGACAGCA GTGCTGAGCC AGTTTTGCTG
    TCTGACGCTA GTAAAGTCAT CTGCCACGGC CCAGGCCTGA GTAAGGGCTT TCTGGGACGC
    AAAAACACTT TTTATGTGGA CTGCAGTAAA GCTGGACGAA ACCTTCTGTT CGTGGGCATG
    CATGGCCCTA CAGTTCCCTG TGAGCGGGTG TCCATCATTC ACATGGGCCA GTGTCAGTAC
    AGGATCAACT ACGCTGTAAA GGAAAGAGGA AAATATGTCT TAGCAGTGAA GTGGGGAGAT
    GAGCACATTC CTGGATCGCC GTTTTATATT AGTGTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.