cpda cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cpda
Entrez Gene ID	563008
Full Name	carboxypeptidase D, a
Synonyms	cpd
General protein information	Preferred Names carboxypeptidase D, a Names carboxypeptidase D carboxypeptidase D, a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005157619.3	XP_005157676.1	carboxypeptidase D isoform X1
XM_005157619.4	XP_005157676.1	carboxypeptidase D isoform X1
XM_021472946.1	XP_021328621.1	carboxypeptidase D isoform X1
XM_021472947.1	XP_021328622.1	carboxypeptidase D isoform X2
XM_021474349.1	XP_021330024.1	carboxypeptidase D isoform X2
XM_686372.8	XP_691464.3	carboxypeptidase D isoform X2
XM_686372.9	XP_691464.3	carboxypeptidase D isoform X2
XM_021474348.1	XP_021330023.1	carboxypeptidase D isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cpd	NP_036968.1
Drosophila melanogaster (fruit fly)	svr	NP_996319.1
Xenopus tropicalis (tropical clawed frog)	LOC101730225	XP_004912387.1
	LOC712407	XP_002808165.1
Canis lupus familiaris (dog)	CPD	XP_537756.2
Mus musculus (house mouse)	Cpd	NP_031780.2
Gallus gallus (chicken)	CPD	XP_004946700.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002414	XP_312538.5
Homo sapiens (human)	CPD	NP_001295.2
Pan troglodytes (chimpanzee)	CPD	XP_003315614.1
Danio rerio (zebrafish)	cpda	XP_691464.3
Caenorhabditis elegans (roundworm)	cpd-2	NP_510625.2

Gene Ontology

The following cpda gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cpda cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa20527	XM_021472946.1 Latest version!	Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa20527	XM_005157619.4 Latest version!	Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa20527	XM_021474348.1 Latest version!	Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa20528	XM_021474349.1 Latest version!	Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa20528	XM_686372.9 Latest version!	Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa20528	XM_021472947.1 Latest version!	Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa20527	XM_005157619.3	Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa20528	XM_686372.8	Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cpda ( XM_005157619.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157676.1 Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20527
Clone ID Related Accession (Same CDS sequence)	XM_005157619.3 , XM_005157619.4 , XM_021472946.1 , XM_021474348.1
Accession Version	XM_005157619.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	carboxypeptidase D isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGTAGGTA ATGCATCTCA GCGAGTTTCA 
GAACCTCAGC CAGAGACACG CGCTGTGATG AATCTGATTC AGGAAAGAGG CTTCACTCTC 
TCTGTGGCTC TGGATGGAGG CTCATTGTTG GTCACTTATC CATATGACAA ACCAGTGCAA 
ACCGTTGAAA ATGATGACAC ACTGAGATAT CTGGCCACTG TGTATGCTGA TCACCATCCC 
ACAATGCACT TGGGTAACAC TGGATGTCCA AACAGCGTCC AAAGTAACAT CCCTGGTGGC 
GTCCTTCGAG CAGCAGTGTG GCACAGTCAC ATGGGCAGCA TGAAGGACTT CAGTGTGGAC 
TTTGGTCACT GTCCCGAGAT CACAGTCTAC ACCAGCTGCT GTCTGTTTCC TTCAGCTGAA 
ATGCTGCCCT CGCTCTGGGC TGAGAACAGG AAGTCTCTTC TGAGCATGCT GGTTGAGGTG 
CACAAAGGGG TTCGTGGAAT AGTCAAGGAT AAGAACGGTA AGCCCATCGT GGGTGCTGCA 
ATTGTGTTGA ACGGTGGTGT TCGAGTCTAC ACTAGTGAAG GAGGGTATTT CCATGCTCTT 
CTGGCTCCTG GTTTACACAG CATTGAAGCG GTGGCTGATG GATACCAACA GCAATCACAG 
AAGGTGTCTG TGTCGCAATT TGAAGCAGCA AGTTCCATTA TCATTGAGTT TGACATGGAG 
AACGATATAT TTGGCCTTCC CAGAGAATTA GTAGTGGCGA GTGCAGCTGC CGCAATGACG 
GCACTAGTCT TGACCGCCTG TATCATCTGG TGCGTCTGCT CGGCCAAATC CAACAGACAT 
AAAGATGGCT TCCATCGACT ACGGCAGCAT CGAGATGATT ATGACGACGA GGTCCGCCTC 
ATGTCTATGG GCTCCAAAAA GTCTCTCCTC AGTCATGAGT TTCAAGATGA GAGCGAAAGT 
GATGAGGATA CACTGTACAC CAACAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157676.1
CDS	152..4204
Translation	MSGQRARDLCSANKCPLLLLSLLVVYISSINSVSAGELKTETYNKYYNYDELTELLKSLAVKHPSIANLTSIGRSVEGRELWVMRVTKDISAADGTGKPKFKYVGNMHGDETVSRQVLVYLLEDLLEKYGEDQRVTELVNTTDIYILPSMNPDGFERSVEGDCLGKDEGRHNAKNIDLNRSFPDQFEEIHLNAEDIPEVTAVIKWILENKFVLSGNLHGGTVVASYPYDDSAGHSTDGTYSRSPDDALFRHLALVYAENNPVMKTGQPKCEDNVNESFPDGITNGAKWYDVPGGMQDFNYLKGNCLEITMELSCCKYPPSSQLKTEWENNRDALLAYMEQIHIGVRGFVRAAKNEAPIADAVIMVADIKHNVSTSRFGDYYRLLLPGTYSITAVAPGYIPMTVAGVEVKEGKATVLNITLLSENDERSTATPNPESSTDTSASSSNSGSTDNNSNSGASDKAADLTIQPQEFRHHRYIDMELFMKKFSSEYSSITRLYSIGKSVQKRLLWVMEISNNPGVHELGEPEFKYIGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVDTTRIHIMPSMNPDGYEVSQKGDVEGIKGRNNSKNYDLNRNFPDRFKLITDPIQPETLAVMNWSKNYSFVLSANLHGGSLVVNYPFDDNLEDVIGYSKSPDDAVFRMVALAYSQENSEMHEGHPCKEMSSYPEYFQDGITNGAAWYNVHGGMQDWNYMNTNCFEVTIELGCHKYPPVADLQKYWEQNRKSLLQFIHQVHRGVKGMVIDSKDGSGIPNATITVETIHHQVTSSSLGDYWRLLVPGKYKLTASAQGYMSDSSSVTVPKDGLEIVNFTLTRIPTHANGQETENTTPVLDPSVREFQALIKQLSTQSGLDKLIQDTPTESSFRYQQYGEVSEFLRGLTLNFPEITSLRSLGQSVEIRNIWALEISNNPKVQEPSKPKIRFVAGIHGNAPVGTELLLEFAESLCVNYGKNAAINRLINETRIVILPSINPDGRELAKERDCTSTVGMTNVHGKDLDTDFIVGNASQRVSEPQPETRAVMNLIQERGFTLSVALDGGSLLVTYPYDKPVQTVENDDTLRYLATVYADHHPTMHLGNTGCPNSVQSNIPGGVLRAAVWHSHMGSMKDFSVDFGHCPEITVYTSCCLFPSAEMLPSLWAENRKSLLSMLVEVHKGVRGIVKDKNGKPIVGAAIVLNGGVRVYTSEGGYFHALLAPGLHSIEAVADGYQQQSQKVSVSQFEAASSIIIEFDMENDIFGLPRELVVASAAAAMTALVLTACIIWCVCSAKSNRHKDGFHRLRQHRDDYDDEVRLMSMGSKKSLLSHEFQDESESDEDTLYTNKL

Target ORF information:

RefSeq Version	XM_005157619.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157619.3

ORF Insert Sequence:

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGTAGGTA ATGCATCTCA GCGAGTTTCA 
GAACCTCAGC CAGAGACACG CGCTGTGATG AATCTGATTC AGGAAAGAGG CTTCACTCTC 
TCTGTGGCTC TGGATGGAGG CTCATTGTTG GTCACTTATC CATATGACAA ACCAGTGCAA 
ACCGTTGAAA ATGATGACAC ACTGAGATAT CTGGCCACTG TGTATGCTGA TCACCATCCC 
ACAATGCACT TGGGTAACAC TGGATGTCCA AACAGCGTCC AAAGTAACAT CCCTGGTGGC 
GTCCTTCGAG CAGCAGTGTG GCACAGTCAC ATGGGCAGCA TGAAGGACTT CAGTGTGGAC 
TTTGGTCACT GTCCCGAGAT CACAGTCTAC ACCAGCTGCT GTCTGTTTCC TTCAGCTGAA 
ATGCTGCCCT CGCTCTGGGC TGAGAACAGG AAGTCTCTTC TGAGCATGCT GGTTGAGGTG 
CACAAAGGGG TTCGTGGAAT AGTCAAGGAT AAGAACGGTA AGCCCATCGT GGGTGCTGCA 
ATTGTGTTGA ACGGTGGTGT TCGAGTCTAC ACTAGTGAAG GAGGGTATTT CCATGCTCTT 
CTGGCTCCTG GTTTACACAG CATTGAAGCG GTGGCTGATG GATACCAACA GCAATCACAG 
AAGGTGTCTG TGTCGCAATT TGAAGCAGCA AGTTCCATTA TCATTGAGTT TGACATGGAG 
AACGATATAT TTGGCCTTCC CAGAGAATTA GTAGTGGCGA GTGCAGCTGC CGCAATGACG 
GCACTAGTCT TGACCGCCTG TATCATCTGG TGCGTCTGCT CGGCCAAATC CAACAGACAT 
AAAGATGGCT TCCATCGACT ACGGCAGCAT CGAGATGATT ATGACGACGA GGTCCGCCTC 
ATGTCTATGG GCTCCAAAAA GTCTCTCCTC AGTCATGAGT TTCAAGATGA GAGCGAAAGT 
GATGAGGATA CACTGTACAC CAACAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpda ( XM_005157619.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157676.1 Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20527
Clone ID Related Accession (Same CDS sequence)	XM_005157619.3 , XM_005157619.4 , XM_021472946.1 , XM_021474348.1
Accession Version	XM_005157619.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157619.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGTAGGTA ATGCATCTCA GCGAGTTTCA 
GAACCTCAGC CAGAGACACG CGCTGTGATG AATCTGATTC AGGAAAGAGG CTTCACTCTC 
TCTGTGGCTC TGGATGGAGG CTCATTGTTG GTCACTTATC CATATGACAA ACCAGTGCAA 
ACCGTTGAAA ATGATGACAC ACTGAGATAT CTGGCCACTG TGTATGCTGA TCACCATCCC 
ACAATGCACT TGGGTAACAC TGGATGTCCA AACAGCGTCC AAAGTAACAT CCCTGGTGGC 
GTCCTTCGAG CAGCAGTGTG GCACAGTCAC ATGGGCAGCA TGAAGGACTT CAGTGTGGAC 
TTTGGTCACT GTCCCGAGAT CACAGTCTAC ACCAGCTGCT GTCTGTTTCC TTCAGCTGAA 
ATGCTGCCCT CGCTCTGGGC TGAGAACAGG AAGTCTCTTC TGAGCATGCT GGTTGAGGTG 
CACAAAGGGG TTCGTGGAAT AGTCAAGGAT AAGAACGGTA AGCCCATCGT GGGTGCTGCA 
ATTGTGTTGA ACGGTGGTGT TCGAGTCTAC ACTAGTGAAG GAGGGTATTT CCATGCTCTT 
CTGGCTCCTG GTTTACACAG CATTGAAGCG GTGGCTGATG GATACCAACA GCAATCACAG 
AAGGTGTCTG TGTCGCAATT TGAAGCAGCA AGTTCCATTA TCATTGAGTT TGACATGGAG 
AACGATATAT TTGGCCTTCC CAGAGAATTA GTAGTGGCGA GTGCAGCTGC CGCAATGACG 
GCACTAGTCT TGACCGCCTG TATCATCTGG TGCGTCTGCT CGGCCAAATC CAACAGACAT 
AAAGATGGCT TCCATCGACT ACGGCAGCAT CGAGATGATT ATGACGACGA GGTCCGCCTC 
ATGTCTATGG GCTCCAAAAA GTCTCTCCTC AGTCATGAGT TTCAAGATGA GAGCGAAAGT 
GATGAGGATA CACTGTACAC CAACAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157676.1
CDS	126..4178
Translation	MSGQRARDLCSANKCPLLLLSLLVVYISSINSVSAGELKTETYNKYYNYDELTELLKSLAVKHPSIANLTSIGRSVEGRELWVMRVTKDISAADGTGKPKFKYVGNMHGDETVSRQVLVYLLEDLLEKYGEDQRVTELVNTTDIYILPSMNPDGFERSVEGDCLGKDEGRHNAKNIDLNRSFPDQFEEIHLNAEDIPEVTAVIKWILENKFVLSGNLHGGTVVASYPYDDSAGHSTDGTYSRSPDDALFRHLALVYAENNPVMKTGQPKCEDNVNESFPDGITNGAKWYDVPGGMQDFNYLKGNCLEITMELSCCKYPPSSQLKTEWENNRDALLAYMEQIHIGVRGFVRAAKNEAPIADAVIMVADIKHNVSTSRFGDYYRLLLPGTYSITAVAPGYIPMTVAGVEVKEGKATVLNITLLSENDERSTATPNPESSTDTSASSSNSGSTDNNSNSGASDKAADLTIQPQEFRHHRYIDMELFMKKFSSEYSSITRLYSIGKSVQKRLLWVMEISNNPGVHELGEPEFKYIGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVDTTRIHIMPSMNPDGYEVSQKGDVEGIKGRNNSKNYDLNRNFPDRFKLITDPIQPETLAVMNWSKNYSFVLSANLHGGSLVVNYPFDDNLEDVIGYSKSPDDAVFRMVALAYSQENSEMHEGHPCKEMSSYPEYFQDGITNGAAWYNVHGGMQDWNYMNTNCFEVTIELGCHKYPPVADLQKYWEQNRKSLLQFIHQVHRGVKGMVIDSKDGSGIPNATITVETIHHQVTSSSLGDYWRLLVPGKYKLTASAQGYMSDSSSVTVPKDGLEIVNFTLTRIPTHANGQETENTTPVLDPSVREFQALIKQLSTQSGLDKLIQDTPTESSFRYQQYGEVSEFLRGLTLNFPEITSLRSLGQSVEIRNIWALEISNNPKVQEPSKPKIRFVAGIHGNAPVGTELLLEFAESLCVNYGKNAAINRLINETRIVILPSINPDGRELAKERDCTSTVGMTNVHGKDLDTDFIVGNASQRVSEPQPETRAVMNLIQERGFTLSVALDGGSLLVTYPYDKPVQTVENDDTLRYLATVYADHHPTMHLGNTGCPNSVQSNIPGGVLRAAVWHSHMGSMKDFSVDFGHCPEITVYTSCCLFPSAEMLPSLWAENRKSLLSMLVEVHKGVRGIVKDKNGKPIVGAAIVLNGGVRVYTSEGGYFHALLAPGLHSIEAVADGYQQQSQKVSVSQFEAASSIIIEFDMENDIFGLPRELVVASAAAAMTALVLTACIIWCVCSAKSNRHKDGFHRLRQHRDDYDDEVRLMSMGSKKSLLSHEFQDESESDEDTLYTNKL

Target ORF information:

RefSeq Version	XM_005157619.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157619.4

ORF Insert Sequence:

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGTAGGTA ATGCATCTCA GCGAGTTTCA 
GAACCTCAGC CAGAGACACG CGCTGTGATG AATCTGATTC AGGAAAGAGG CTTCACTCTC 
TCTGTGGCTC TGGATGGAGG CTCATTGTTG GTCACTTATC CATATGACAA ACCAGTGCAA 
ACCGTTGAAA ATGATGACAC ACTGAGATAT CTGGCCACTG TGTATGCTGA TCACCATCCC 
ACAATGCACT TGGGTAACAC TGGATGTCCA AACAGCGTCC AAAGTAACAT CCCTGGTGGC 
GTCCTTCGAG CAGCAGTGTG GCACAGTCAC ATGGGCAGCA TGAAGGACTT CAGTGTGGAC 
TTTGGTCACT GTCCCGAGAT CACAGTCTAC ACCAGCTGCT GTCTGTTTCC TTCAGCTGAA 
ATGCTGCCCT CGCTCTGGGC TGAGAACAGG AAGTCTCTTC TGAGCATGCT GGTTGAGGTG 
CACAAAGGGG TTCGTGGAAT AGTCAAGGAT AAGAACGGTA AGCCCATCGT GGGTGCTGCA 
ATTGTGTTGA ACGGTGGTGT TCGAGTCTAC ACTAGTGAAG GAGGGTATTT CCATGCTCTT 
CTGGCTCCTG GTTTACACAG CATTGAAGCG GTGGCTGATG GATACCAACA GCAATCACAG 
AAGGTGTCTG TGTCGCAATT TGAAGCAGCA AGTTCCATTA TCATTGAGTT TGACATGGAG 
AACGATATAT TTGGCCTTCC CAGAGAATTA GTAGTGGCGA GTGCAGCTGC CGCAATGACG 
GCACTAGTCT TGACCGCCTG TATCATCTGG TGCGTCTGCT CGGCCAAATC CAACAGACAT 
AAAGATGGCT TCCATCGACT ACGGCAGCAT CGAGATGATT ATGACGACGA GGTCCGCCTC 
ATGTCTATGG GCTCCAAAAA GTCTCTCCTC AGTCATGAGT TTCAAGATGA GAGCGAAAGT 
GATGAGGATA CACTGTACAC CAACAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpda ( XM_021472946.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328621.1 Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.

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CloneID	ODa20527
Clone ID Related Accession (Same CDS sequence)	XM_005157619.3 , XM_005157619.4 , XM_021472946.1 , XM_021474348.1
Accession Version	XM_021472946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394947.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGTAGGTA ATGCATCTCA GCGAGTTTCA 
GAACCTCAGC CAGAGACACG CGCTGTGATG AATCTGATTC AGGAAAGAGG CTTCACTCTC 
TCTGTGGCTC TGGATGGAGG CTCATTGTTG GTCACTTATC CATATGACAA ACCAGTGCAA 
ACCGTTGAAA ATGATGACAC ACTGAGATAT CTGGCCACTG TGTATGCTGA TCACCATCCC 
ACAATGCACT TGGGTAACAC TGGATGTCCA AACAGCGTCC AAAGTAACAT CCCTGGTGGC 
GTCCTTCGAG CAGCAGTGTG GCACAGTCAC ATGGGCAGCA TGAAGGACTT CAGTGTGGAC 
TTTGGTCACT GTCCCGAGAT CACAGTCTAC ACCAGCTGCT GTCTGTTTCC TTCAGCTGAA 
ATGCTGCCCT CGCTCTGGGC TGAGAACAGG AAGTCTCTTC TGAGCATGCT GGTTGAGGTG 
CACAAAGGGG TTCGTGGAAT AGTCAAGGAT AAGAACGGTA AGCCCATCGT GGGTGCTGCA 
ATTGTGTTGA ACGGTGGTGT TCGAGTCTAC ACTAGTGAAG GAGGGTATTT CCATGCTCTT 
CTGGCTCCTG GTTTACACAG CATTGAAGCG GTGGCTGATG GATACCAACA GCAATCACAG 
AAGGTGTCTG TGTCGCAATT TGAAGCAGCA AGTTCCATTA TCATTGAGTT TGACATGGAG 
AACGATATAT TTGGCCTTCC CAGAGAATTA GTAGTGGCGA GTGCAGCTGC CGCAATGACG 
GCACTAGTCT TGACCGCCTG TATCATCTGG TGCGTCTGCT CGGCCAAATC CAACAGACAT 
AAAGATGGCT TCCATCGACT ACGGCAGCAT CGAGATGATT ATGACGACGA GGTCCGCCTC 
ATGTCTATGG GCTCCAAAAA GTCTCTCCTC AGTCATGAGT TTCAAGATGA GAGCGAAAGT 
GATGAGGATA CACTGTACAC CAACAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328621.1
CDS	126..4178
Translation	MSGQRARDLCSANKCPLLLLSLLVVYISSINSVSAGELKTETYNKYYNYDELTELLKSLAVKHPSIANLTSIGRSVEGRELWVMRVTKDISAADGTGKPKFKYVGNMHGDETVSRQVLVYLLEDLLEKYGEDQRVTELVNTTDIYILPSMNPDGFERSVEGDCLGKDEGRHNAKNIDLNRSFPDQFEEIHLNAEDIPEVTAVIKWILENKFVLSGNLHGGTVVASYPYDDSAGHSTDGTYSRSPDDALFRHLALVYAENNPVMKTGQPKCEDNVNESFPDGITNGAKWYDVPGGMQDFNYLKGNCLEITMELSCCKYPPSSQLKTEWENNRDALLAYMEQIHIGVRGFVRAAKNEAPIADAVIMVADIKHNVSTSRFGDYYRLLLPGTYSITAVAPGYIPMTVAGVEVKEGKATVLNITLLSENDERSTATPNPESSTDTSASSSNSGSTDNNSNSGASDKAADLTIQPQEFRHHRYIDMELFMKKFSSEYSSITRLYSIGKSVQKRLLWVMEISNNPGVHELGEPEFKYIGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVDTTRIHIMPSMNPDGYEVSQKGDVEGIKGRNNSKNYDLNRNFPDRFKLITDPIQPETLAVMNWSKNYSFVLSANLHGGSLVVNYPFDDNLEDVIGYSKSPDDAVFRMVALAYSQENSEMHEGHPCKEMSSYPEYFQDGITNGAAWYNVHGGMQDWNYMNTNCFEVTIELGCHKYPPVADLQKYWEQNRKSLLQFIHQVHRGVKGMVIDSKDGSGIPNATITVETIHHQVTSSSLGDYWRLLVPGKYKLTASAQGYMSDSSSVTVPKDGLEIVNFTLTRIPTHANGQETENTTPVLDPSVREFQALIKQLSTQSGLDKLIQDTPTESSFRYQQYGEVSEFLRGLTLNFPEITSLRSLGQSVEIRNIWALEISNNPKVQEPSKPKIRFVAGIHGNAPVGTELLLEFAESLCVNYGKNAAINRLINETRIVILPSINPDGRELAKERDCTSTVGMTNVHGKDLDTDFIVGNASQRVSEPQPETRAVMNLIQERGFTLSVALDGGSLLVTYPYDKPVQTVENDDTLRYLATVYADHHPTMHLGNTGCPNSVQSNIPGGVLRAAVWHSHMGSMKDFSVDFGHCPEITVYTSCCLFPSAEMLPSLWAENRKSLLSMLVEVHKGVRGIVKDKNGKPIVGAAIVLNGGVRVYTSEGGYFHALLAPGLHSIEAVADGYQQQSQKVSVSQFEAASSIIIEFDMENDIFGLPRELVVASAAAAMTALVLTACIIWCVCSAKSNRHKDGFHRLRQHRDDYDDEVRLMSMGSKKSLLSHEFQDESESDEDTLYTNKL

Target ORF information:

RefSeq Version	XM_021472946.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472946.1

ORF Insert Sequence:

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGTAGGTA ATGCATCTCA GCGAGTTTCA 
GAACCTCAGC CAGAGACACG CGCTGTGATG AATCTGATTC AGGAAAGAGG CTTCACTCTC 
TCTGTGGCTC TGGATGGAGG CTCATTGTTG GTCACTTATC CATATGACAA ACCAGTGCAA 
ACCGTTGAAA ATGATGACAC ACTGAGATAT CTGGCCACTG TGTATGCTGA TCACCATCCC 
ACAATGCACT TGGGTAACAC TGGATGTCCA AACAGCGTCC AAAGTAACAT CCCTGGTGGC 
GTCCTTCGAG CAGCAGTGTG GCACAGTCAC ATGGGCAGCA TGAAGGACTT CAGTGTGGAC 
TTTGGTCACT GTCCCGAGAT CACAGTCTAC ACCAGCTGCT GTCTGTTTCC TTCAGCTGAA 
ATGCTGCCCT CGCTCTGGGC TGAGAACAGG AAGTCTCTTC TGAGCATGCT GGTTGAGGTG 
CACAAAGGGG TTCGTGGAAT AGTCAAGGAT AAGAACGGTA AGCCCATCGT GGGTGCTGCA 
ATTGTGTTGA ACGGTGGTGT TCGAGTCTAC ACTAGTGAAG GAGGGTATTT CCATGCTCTT 
CTGGCTCCTG GTTTACACAG CATTGAAGCG GTGGCTGATG GATACCAACA GCAATCACAG 
AAGGTGTCTG TGTCGCAATT TGAAGCAGCA AGTTCCATTA TCATTGAGTT TGACATGGAG 
AACGATATAT TTGGCCTTCC CAGAGAATTA GTAGTGGCGA GTGCAGCTGC CGCAATGACG 
GCACTAGTCT TGACCGCCTG TATCATCTGG TGCGTCTGCT CGGCCAAATC CAACAGACAT 
AAAGATGGCT TCCATCGACT ACGGCAGCAT CGAGATGATT ATGACGACGA GGTCCGCCTC 
ATGTCTATGG GCTCCAAAAA GTCTCTCCTC AGTCATGAGT TTCAAGATGA GAGCGAAAGT 
GATGAGGATA CACTGTACAC CAACAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpda ( XM_021474348.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330023.1 Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.

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CloneID	ODa20527
Clone ID Related Accession (Same CDS sequence)	XM_005157619.3 , XM_005157619.4 , XM_021472946.1 , XM_021474348.1
Accession Version	XM_021474348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395323.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGTAGGTA ATGCATCTCA GCGAGTTTCA 
GAACCTCAGC CAGAGACACG CGCTGTGATG AATCTGATTC AGGAAAGAGG CTTCACTCTC 
TCTGTGGCTC TGGATGGAGG CTCATTGTTG GTCACTTATC CATATGACAA ACCAGTGCAA 
ACCGTTGAAA ATGATGACAC ACTGAGATAT CTGGCCACTG TGTATGCTGA TCACCATCCC 
ACAATGCACT TGGGTAACAC TGGATGTCCA AACAGCGTCC AAAGTAACAT CCCTGGTGGC 
GTCCTTCGAG CAGCAGTGTG GCACAGTCAC ATGGGCAGCA TGAAGGACTT CAGTGTGGAC 
TTTGGTCACT GTCCCGAGAT CACAGTCTAC ACCAGCTGCT GTCTGTTTCC TTCAGCTGAA 
ATGCTGCCCT CGCTCTGGGC TGAGAACAGG AAGTCTCTTC TGAGCATGCT GGTTGAGGTG 
CACAAAGGGG TTCGTGGAAT AGTCAAGGAT AAGAACGGTA AGCCCATCGT GGGTGCTGCA 
ATTGTGTTGA ACGGTGGTGT TCGAGTCTAC ACTAGTGAAG GAGGGTATTT CCATGCTCTT 
CTGGCTCCTG GTTTACACAG CATTGAAGCG GTGGCTGATG GATACCAACA GCAATCACAG 
AAGGTGTCTG TGTCGCAATT TGAAGCAGCA AGTTCCATTA TCATTGAGTT TGACATGGAG 
AACGATATAT TTGGCCTTCC CAGAGAATTA GTAGTGGCGA GTGCAGCTGC CGCAATGACG 
GCACTAGTCT TGACCGCCTG TATCATCTGG TGCGTCTGCT CGGCCAAATC CAACAGACAT 
AAAGATGGCT TCCATCGACT ACGGCAGCAT CGAGATGATT ATGACGACGA GGTCCGCCTC 
ATGTCTATGG GCTCCAAAAA GTCTCTCCTC AGTCATGAGT TTCAAGATGA GAGCGAAAGT 
GATGAGGATA CACTGTACAC CAACAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330023.1
CDS	126..4178
Translation	MSGQRARDLCSANKCPLLLLSLLVVYISSINSVSAGELKTETYNKYYNYDELTELLKSLAVKHPSIANLTSIGRSVEGRELWVMRVTKDISAADGTGKPKFKYVGNMHGDETVSRQVLVYLLEDLLEKYGEDQRVTELVNTTDIYILPSMNPDGFERSVEGDCLGKDEGRHNAKNIDLNRSFPDQFEEIHLNAEDIPEVTAVIKWILENKFVLSGNLHGGTVVASYPYDDSAGHSTDGTYSRSPDDALFRHLALVYAENNPVMKTGQPKCEDNVNESFPDGITNGAKWYDVPGGMQDFNYLKGNCLEITMELSCCKYPPSSQLKTEWENNRDALLAYMEQIHIGVRGFVRAAKNEAPIADAVIMVADIKHNVSTSRFGDYYRLLLPGTYSITAVAPGYIPMTVAGVEVKEGKATVLNITLLSENDERSTATPNPESSTDTSASSSNSGSTDNNSNSGASDKAADLTIQPQEFRHHRYIDMELFMKKFSSEYSSITRLYSIGKSVQKRLLWVMEISNNPGVHELGEPEFKYIGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVDTTRIHIMPSMNPDGYEVSQKGDVEGIKGRNNSKNYDLNRNFPDRFKLITDPIQPETLAVMNWSKNYSFVLSANLHGGSLVVNYPFDDNLEDVIGYSKSPDDAVFRMVALAYSQENSEMHEGHPCKEMSSYPEYFQDGITNGAAWYNVHGGMQDWNYMNTNCFEVTIELGCHKYPPVADLQKYWEQNRKSLLQFIHQVHRGVKGMVIDSKDGSGIPNATITVETIHHQVTSSSLGDYWRLLVPGKYKLTASAQGYMSDSSSVTVPKDGLEIVNFTLTRIPTHANGQETENTTPVLDPSVREFQALIKQLSTQSGLDKLIQDTPTESSFRYQQYGEVSEFLRGLTLNFPEITSLRSLGQSVEIRNIWALEISNNPKVQEPSKPKIRFVAGIHGNAPVGTELLLEFAESLCVNYGKNAAINRLINETRIVILPSINPDGRELAKERDCTSTVGMTNVHGKDLDTDFIVGNASQRVSEPQPETRAVMNLIQERGFTLSVALDGGSLLVTYPYDKPVQTVENDDTLRYLATVYADHHPTMHLGNTGCPNSVQSNIPGGVLRAAVWHSHMGSMKDFSVDFGHCPEITVYTSCCLFPSAEMLPSLWAENRKSLLSMLVEVHKGVRGIVKDKNGKPIVGAAIVLNGGVRVYTSEGGYFHALLAPGLHSIEAVADGYQQQSQKVSVSQFEAASSIIIEFDMENDIFGLPRELVVASAAAAMTALVLTACIIWCVCSAKSNRHKDGFHRLRQHRDDYDDEVRLMSMGSKKSLLSHEFQDESESDEDTLYTNKL

Target ORF information:

RefSeq Version	XM_021474348.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase D, a (cpda), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474348.1

ORF Insert Sequence:

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGTAGGTA ATGCATCTCA GCGAGTTTCA 
GAACCTCAGC CAGAGACACG CGCTGTGATG AATCTGATTC AGGAAAGAGG CTTCACTCTC 
TCTGTGGCTC TGGATGGAGG CTCATTGTTG GTCACTTATC CATATGACAA ACCAGTGCAA 
ACCGTTGAAA ATGATGACAC ACTGAGATAT CTGGCCACTG TGTATGCTGA TCACCATCCC 
ACAATGCACT TGGGTAACAC TGGATGTCCA AACAGCGTCC AAAGTAACAT CCCTGGTGGC 
GTCCTTCGAG CAGCAGTGTG GCACAGTCAC ATGGGCAGCA TGAAGGACTT CAGTGTGGAC 
TTTGGTCACT GTCCCGAGAT CACAGTCTAC ACCAGCTGCT GTCTGTTTCC TTCAGCTGAA 
ATGCTGCCCT CGCTCTGGGC TGAGAACAGG AAGTCTCTTC TGAGCATGCT GGTTGAGGTG 
CACAAAGGGG TTCGTGGAAT AGTCAAGGAT AAGAACGGTA AGCCCATCGT GGGTGCTGCA 
ATTGTGTTGA ACGGTGGTGT TCGAGTCTAC ACTAGTGAAG GAGGGTATTT CCATGCTCTT 
CTGGCTCCTG GTTTACACAG CATTGAAGCG GTGGCTGATG GATACCAACA GCAATCACAG 
AAGGTGTCTG TGTCGCAATT TGAAGCAGCA AGTTCCATTA TCATTGAGTT TGACATGGAG 
AACGATATAT TTGGCCTTCC CAGAGAATTA GTAGTGGCGA GTGCAGCTGC CGCAATGACG 
GCACTAGTCT TGACCGCCTG TATCATCTGG TGCGTCTGCT CGGCCAAATC CAACAGACAT 
AAAGATGGCT TCCATCGACT ACGGCAGCAT CGAGATGATT ATGACGACGA GGTCCGCCTC 
ATGTCTATGG GCTCCAAAAA GTCTCTCCTC AGTCATGAGT TTCAAGATGA GAGCGAAAGT 
GATGAGGATA CACTGTACAC CAACAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpda ( XM_021472947.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328622.1 Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20528
Clone ID Related Accession (Same CDS sequence)	XM_021472947.1 , XM_021474349.1 , XM_686372.8 , XM_686372.9
Accession Version	XM_021472947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394947.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGGTAATG CATCTCAGCG AGTTTCAGAA 
CCTCAGCCAG AGACACGCGC TGTGATGAAT CTGATTCAGG AAAGAGGCTT CACTCTCTCT 
GTGGCTCTGG ATGGAGGCTC ATTGTTGGTC ACTTATCCAT ATGACAAACC AGTGCAAACC 
GTTGAAAATG ATGACACACT GAGATATCTG GCCACTGTGT ATGCTGATCA CCATCCCACA 
ATGCACTTGG GTAACACTGG ATGTCCAAAC AGCGTCCAAA GTAACATCCC TGGTGGCGTC 
CTTCGAGCAG CAGTGTGGCA CAGTCACATG GGCAGCATGA AGGACTTCAG TGTGGACTTT 
GGTCACTGTC CCGAGATCAC AGTCTACACC AGCTGCTGTC TGTTTCCTTC AGCTGAAATG 
CTGCCCTCGC TCTGGGCTGA GAACAGGAAG TCTCTTCTGA GCATGCTGGT TGAGGTGCAC 
AAAGGGGTTC GTGGAATAGT CAAGGATAAG AACGGTAAGC CCATCGTGGG TGCTGCAATT 
GTGTTGAACG GTGGTGTTCG AGTCTACACT AGTGAAGGAG GGTATTTCCA TGCTCTTCTG 
GCTCCTGGTT TACACAGCAT TGAAGCGGTG GCTGATGGAT ACCAACAGCA ATCACAGAAG 
GTGTCTGTGT CGCAATTTGA AGCAGCAAGT TCCATTATCA TTGAGTTTGA CATGGAGAAC 
GATATATTTG GCCTTCCCAG AGAATTAGTA GTGGCGAGTG CAGCTGCCGC AATGACGGCA 
CTAGTCTTGA CCGCCTGTAT CATCTGGTGC GTCTGCTCGG CCAAATCCAA CAGACATAAA 
GATGGCTTCC ATCGACTACG GCAGCATCGA GATGATTATG ACGACGAGGT CCGCCTCATG 
TCTATGGGCT CCAAAAAGTC TCTCCTCAGT CATGAGTTTC AAGATGAGAG CGAAAGTGAT 
GAGGATACAC TGTACACCAA CAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328622.1
CDS	126..4175
Translation	MSGQRARDLCSANKCPLLLLSLLVVYISSINSVSAGELKTETYNKYYNYDELTELLKSLAVKHPSIANLTSIGRSVEGRELWVMRVTKDISAADGTGKPKFKYVGNMHGDETVSRQVLVYLLEDLLEKYGEDQRVTELVNTTDIYILPSMNPDGFERSVEGDCLGKDEGRHNAKNIDLNRSFPDQFEEIHLNAEDIPEVTAVIKWILENKFVLSGNLHGGTVVASYPYDDSAGHSTDGTYSRSPDDALFRHLALVYAENNPVMKTGQPKCEDNVNESFPDGITNGAKWYDVPGGMQDFNYLKGNCLEITMELSCCKYPPSSQLKTEWENNRDALLAYMEQIHIGVRGFVRAAKNEAPIADAVIMVADIKHNVSTSRFGDYYRLLLPGTYSITAVAPGYIPMTVAGVEVKEGKATVLNITLLSENDERSTATPNPESSTDTSASSSNSGSTDNNSNSGASDKAADLTIQPQEFRHHRYIDMELFMKKFSSEYSSITRLYSIGKSVQKRLLWVMEISNNPGVHELGEPEFKYIGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVDTTRIHIMPSMNPDGYEVSQKGDVEGIKGRNNSKNYDLNRNFPDRFKLITDPIQPETLAVMNWSKNYSFVLSANLHGGSLVVNYPFDDNLEDVIGYSKSPDDAVFRMVALAYSQENSEMHEGHPCKEMSSYPEYFQDGITNGAAWYNVHGGMQDWNYMNTNCFEVTIELGCHKYPPVADLQKYWEQNRKSLLQFIHQVHRGVKGMVIDSKDGSGIPNATITVETIHHQVTSSSLGDYWRLLVPGKYKLTASAQGYMSDSSSVTVPKDGLEIVNFTLTRIPTHANGQETENTTPVLDPSVREFQALIKQLSTQSGLDKLIQDTPTESSFRYQQYGEVSEFLRGLTLNFPEITSLRSLGQSVEIRNIWALEISNNPKVQEPSKPKIRFVAGIHGNAPVGTELLLEFAESLCVNYGKNAAINRLINETRIVILPSINPDGRELAKERDCTSTVGMTNVHGKDLDTDFIGNASQRVSEPQPETRAVMNLIQERGFTLSVALDGGSLLVTYPYDKPVQTVENDDTLRYLATVYADHHPTMHLGNTGCPNSVQSNIPGGVLRAAVWHSHMGSMKDFSVDFGHCPEITVYTSCCLFPSAEMLPSLWAENRKSLLSMLVEVHKGVRGIVKDKNGKPIVGAAIVLNGGVRVYTSEGGYFHALLAPGLHSIEAVADGYQQQSQKVSVSQFEAASSIIIEFDMENDIFGLPRELVVASAAAAMTALVLTACIIWCVCSAKSNRHKDGFHRLRQHRDDYDDEVRLMSMGSKKSLLSHEFQDESESDEDTLYTNKL

Target ORF information:

RefSeq Version	XM_021472947.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472947.1

ORF Insert Sequence:

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGGTAATG CATCTCAGCG AGTTTCAGAA 
CCTCAGCCAG AGACACGCGC TGTGATGAAT CTGATTCAGG AAAGAGGCTT CACTCTCTCT 
GTGGCTCTGG ATGGAGGCTC ATTGTTGGTC ACTTATCCAT ATGACAAACC AGTGCAAACC 
GTTGAAAATG ATGACACACT GAGATATCTG GCCACTGTGT ATGCTGATCA CCATCCCACA 
ATGCACTTGG GTAACACTGG ATGTCCAAAC AGCGTCCAAA GTAACATCCC TGGTGGCGTC 
CTTCGAGCAG CAGTGTGGCA CAGTCACATG GGCAGCATGA AGGACTTCAG TGTGGACTTT 
GGTCACTGTC CCGAGATCAC AGTCTACACC AGCTGCTGTC TGTTTCCTTC AGCTGAAATG 
CTGCCCTCGC TCTGGGCTGA GAACAGGAAG TCTCTTCTGA GCATGCTGGT TGAGGTGCAC 
AAAGGGGTTC GTGGAATAGT CAAGGATAAG AACGGTAAGC CCATCGTGGG TGCTGCAATT 
GTGTTGAACG GTGGTGTTCG AGTCTACACT AGTGAAGGAG GGTATTTCCA TGCTCTTCTG 
GCTCCTGGTT TACACAGCAT TGAAGCGGTG GCTGATGGAT ACCAACAGCA ATCACAGAAG 
GTGTCTGTGT CGCAATTTGA AGCAGCAAGT TCCATTATCA TTGAGTTTGA CATGGAGAAC 
GATATATTTG GCCTTCCCAG AGAATTAGTA GTGGCGAGTG CAGCTGCCGC AATGACGGCA 
CTAGTCTTGA CCGCCTGTAT CATCTGGTGC GTCTGCTCGG CCAAATCCAA CAGACATAAA 
GATGGCTTCC ATCGACTACG GCAGCATCGA GATGATTATG ACGACGAGGT CCGCCTCATG 
TCTATGGGCT CCAAAAAGTC TCTCCTCAGT CATGAGTTTC AAGATGAGAG CGAAAGTGAT 
GAGGATACAC TGTACACCAA CAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpda ( XM_021474349.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330024.1 Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20528
Clone ID Related Accession (Same CDS sequence)	XM_021472947.1 , XM_021474349.1 , XM_686372.8 , XM_686372.9
Accession Version	XM_021474349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395323.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGGTAATG CATCTCAGCG AGTTTCAGAA 
CCTCAGCCAG AGACACGCGC TGTGATGAAT CTGATTCAGG AAAGAGGCTT CACTCTCTCT 
GTGGCTCTGG ATGGAGGCTC ATTGTTGGTC ACTTATCCAT ATGACAAACC AGTGCAAACC 
GTTGAAAATG ATGACACACT GAGATATCTG GCCACTGTGT ATGCTGATCA CCATCCCACA 
ATGCACTTGG GTAACACTGG ATGTCCAAAC AGCGTCCAAA GTAACATCCC TGGTGGCGTC 
CTTCGAGCAG CAGTGTGGCA CAGTCACATG GGCAGCATGA AGGACTTCAG TGTGGACTTT 
GGTCACTGTC CCGAGATCAC AGTCTACACC AGCTGCTGTC TGTTTCCTTC AGCTGAAATG 
CTGCCCTCGC TCTGGGCTGA GAACAGGAAG TCTCTTCTGA GCATGCTGGT TGAGGTGCAC 
AAAGGGGTTC GTGGAATAGT CAAGGATAAG AACGGTAAGC CCATCGTGGG TGCTGCAATT 
GTGTTGAACG GTGGTGTTCG AGTCTACACT AGTGAAGGAG GGTATTTCCA TGCTCTTCTG 
GCTCCTGGTT TACACAGCAT TGAAGCGGTG GCTGATGGAT ACCAACAGCA ATCACAGAAG 
GTGTCTGTGT CGCAATTTGA AGCAGCAAGT TCCATTATCA TTGAGTTTGA CATGGAGAAC 
GATATATTTG GCCTTCCCAG AGAATTAGTA GTGGCGAGTG CAGCTGCCGC AATGACGGCA 
CTAGTCTTGA CCGCCTGTAT CATCTGGTGC GTCTGCTCGG CCAAATCCAA CAGACATAAA 
GATGGCTTCC ATCGACTACG GCAGCATCGA GATGATTATG ACGACGAGGT CCGCCTCATG 
TCTATGGGCT CCAAAAAGTC TCTCCTCAGT CATGAGTTTC AAGATGAGAG CGAAAGTGAT 
GAGGATACAC TGTACACCAA CAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330024.1
CDS	126..4175
Translation	MSGQRARDLCSANKCPLLLLSLLVVYISSINSVSAGELKTETYNKYYNYDELTELLKSLAVKHPSIANLTSIGRSVEGRELWVMRVTKDISAADGTGKPKFKYVGNMHGDETVSRQVLVYLLEDLLEKYGEDQRVTELVNTTDIYILPSMNPDGFERSVEGDCLGKDEGRHNAKNIDLNRSFPDQFEEIHLNAEDIPEVTAVIKWILENKFVLSGNLHGGTVVASYPYDDSAGHSTDGTYSRSPDDALFRHLALVYAENNPVMKTGQPKCEDNVNESFPDGITNGAKWYDVPGGMQDFNYLKGNCLEITMELSCCKYPPSSQLKTEWENNRDALLAYMEQIHIGVRGFVRAAKNEAPIADAVIMVADIKHNVSTSRFGDYYRLLLPGTYSITAVAPGYIPMTVAGVEVKEGKATVLNITLLSENDERSTATPNPESSTDTSASSSNSGSTDNNSNSGASDKAADLTIQPQEFRHHRYIDMELFMKKFSSEYSSITRLYSIGKSVQKRLLWVMEISNNPGVHELGEPEFKYIGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVDTTRIHIMPSMNPDGYEVSQKGDVEGIKGRNNSKNYDLNRNFPDRFKLITDPIQPETLAVMNWSKNYSFVLSANLHGGSLVVNYPFDDNLEDVIGYSKSPDDAVFRMVALAYSQENSEMHEGHPCKEMSSYPEYFQDGITNGAAWYNVHGGMQDWNYMNTNCFEVTIELGCHKYPPVADLQKYWEQNRKSLLQFIHQVHRGVKGMVIDSKDGSGIPNATITVETIHHQVTSSSLGDYWRLLVPGKYKLTASAQGYMSDSSSVTVPKDGLEIVNFTLTRIPTHANGQETENTTPVLDPSVREFQALIKQLSTQSGLDKLIQDTPTESSFRYQQYGEVSEFLRGLTLNFPEITSLRSLGQSVEIRNIWALEISNNPKVQEPSKPKIRFVAGIHGNAPVGTELLLEFAESLCVNYGKNAAINRLINETRIVILPSINPDGRELAKERDCTSTVGMTNVHGKDLDTDFIGNASQRVSEPQPETRAVMNLIQERGFTLSVALDGGSLLVTYPYDKPVQTVENDDTLRYLATVYADHHPTMHLGNTGCPNSVQSNIPGGVLRAAVWHSHMGSMKDFSVDFGHCPEITVYTSCCLFPSAEMLPSLWAENRKSLLSMLVEVHKGVRGIVKDKNGKPIVGAAIVLNGGVRVYTSEGGYFHALLAPGLHSIEAVADGYQQQSQKVSVSQFEAASSIIIEFDMENDIFGLPRELVVASAAAAMTALVLTACIIWCVCSAKSNRHKDGFHRLRQHRDDYDDEVRLMSMGSKKSLLSHEFQDESESDEDTLYTNKL

Target ORF information:

RefSeq Version	XM_021474349.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474349.1

ORF Insert Sequence:

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGGTAATG CATCTCAGCG AGTTTCAGAA 
CCTCAGCCAG AGACACGCGC TGTGATGAAT CTGATTCAGG AAAGAGGCTT CACTCTCTCT 
GTGGCTCTGG ATGGAGGCTC ATTGTTGGTC ACTTATCCAT ATGACAAACC AGTGCAAACC 
GTTGAAAATG ATGACACACT GAGATATCTG GCCACTGTGT ATGCTGATCA CCATCCCACA 
ATGCACTTGG GTAACACTGG ATGTCCAAAC AGCGTCCAAA GTAACATCCC TGGTGGCGTC 
CTTCGAGCAG CAGTGTGGCA CAGTCACATG GGCAGCATGA AGGACTTCAG TGTGGACTTT 
GGTCACTGTC CCGAGATCAC AGTCTACACC AGCTGCTGTC TGTTTCCTTC AGCTGAAATG 
CTGCCCTCGC TCTGGGCTGA GAACAGGAAG TCTCTTCTGA GCATGCTGGT TGAGGTGCAC 
AAAGGGGTTC GTGGAATAGT CAAGGATAAG AACGGTAAGC CCATCGTGGG TGCTGCAATT 
GTGTTGAACG GTGGTGTTCG AGTCTACACT AGTGAAGGAG GGTATTTCCA TGCTCTTCTG 
GCTCCTGGTT TACACAGCAT TGAAGCGGTG GCTGATGGAT ACCAACAGCA ATCACAGAAG 
GTGTCTGTGT CGCAATTTGA AGCAGCAAGT TCCATTATCA TTGAGTTTGA CATGGAGAAC 
GATATATTTG GCCTTCCCAG AGAATTAGTA GTGGCGAGTG CAGCTGCCGC AATGACGGCA 
CTAGTCTTGA CCGCCTGTAT CATCTGGTGC GTCTGCTCGG CCAAATCCAA CAGACATAAA 
GATGGCTTCC ATCGACTACG GCAGCATCGA GATGATTATG ACGACGAGGT CCGCCTCATG 
TCTATGGGCT CCAAAAAGTC TCTCCTCAGT CATGAGTTTC AAGATGAGAG CGAAAGTGAT 
GAGGATACAC TGTACACCAA CAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpda ( XM_686372.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_691464.3 Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.

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CloneID	ODa20528
Clone ID Related Accession (Same CDS sequence)	XM_021472947.1 , XM_021474349.1 , XM_686372.8 , XM_686372.9
Accession Version	XM_686372.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	carboxypeptidase D isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_686372.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGGTAATG CATCTCAGCG AGTTTCAGAA 
CCTCAGCCAG AGACACGCGC TGTGATGAAT CTGATTCAGG AAAGAGGCTT CACTCTCTCT 
GTGGCTCTGG ATGGAGGCTC ATTGTTGGTC ACTTATCCAT ATGACAAACC AGTGCAAACC 
GTTGAAAATG ATGACACACT GAGATATCTG GCCACTGTGT ATGCTGATCA CCATCCCACA 
ATGCACTTGG GTAACACTGG ATGTCCAAAC AGCGTCCAAA GTAACATCCC TGGTGGCGTC 
CTTCGAGCAG CAGTGTGGCA CAGTCACATG GGCAGCATGA AGGACTTCAG TGTGGACTTT 
GGTCACTGTC CCGAGATCAC AGTCTACACC AGCTGCTGTC TGTTTCCTTC AGCTGAAATG 
CTGCCCTCGC TCTGGGCTGA GAACAGGAAG TCTCTTCTGA GCATGCTGGT TGAGGTGCAC 
AAAGGGGTTC GTGGAATAGT CAAGGATAAG AACGGTAAGC CCATCGTGGG TGCTGCAATT 
GTGTTGAACG GTGGTGTTCG AGTCTACACT AGTGAAGGAG GGTATTTCCA TGCTCTTCTG 
GCTCCTGGTT TACACAGCAT TGAAGCGGTG GCTGATGGAT ACCAACAGCA ATCACAGAAG 
GTGTCTGTGT CGCAATTTGA AGCAGCAAGT TCCATTATCA TTGAGTTTGA CATGGAGAAC 
GATATATTTG GCCTTCCCAG AGAATTAGTA GTGGCGAGTG CAGCTGCCGC AATGACGGCA 
CTAGTCTTGA CCGCCTGTAT CATCTGGTGC GTCTGCTCGG CCAAATCCAA CAGACATAAA 
GATGGCTTCC ATCGACTACG GCAGCATCGA GATGATTATG ACGACGAGGT CCGCCTCATG 
TCTATGGGCT CCAAAAAGTC TCTCCTCAGT CATGAGTTTC AAGATGAGAG CGAAAGTGAT 
GAGGATACAC TGTACACCAA CAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_691464.3
CDS	153..4202
Translation	MSGQRARDLCSANKCPLLLLSLLVVYISSINSVSAGELKTETYNKYYNYDELTELLKSLAVKHPSIANLTSIGRSVEGRELWVMRVTKDISAADGTGKPKFKYVGNMHGDETVSRQVLVYLLEDLLEKYGEDQRVTELVNTTDIYILPSMNPDGFERSVEGDCLGKDEGRHNAKNIDLNRSFPDQFEEIHLNAEDIPEVTAVIKWILENKFVLSGNLHGGTVVASYPYDDSAGHSTDGTYSRSPDDALFRHLALVYAENNPVMKTGQPKCEDNVNESFPDGITNGAKWYDVPGGMQDFNYLKGNCLEITMELSCCKYPPSSQLKTEWENNRDALLAYMEQIHIGVRGFVRAAKNEAPIADAVIMVADIKHNVSTSRFGDYYRLLLPGTYSITAVAPGYIPMTVAGVEVKEGKATVLNITLLSENDERSTATPNPESSTDTSASSSNSGSTDNNSNSGASDKAADLTIQPQEFRHHRYIDMELFMKKFSSEYSSITRLYSIGKSVQKRLLWVMEISNNPGVHELGEPEFKYIGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVDTTRIHIMPSMNPDGYEVSQKGDVEGIKGRNNSKNYDLNRNFPDRFKLITDPIQPETLAVMNWSKNYSFVLSANLHGGSLVVNYPFDDNLEDVIGYSKSPDDAVFRMVALAYSQENSEMHEGHPCKEMSSYPEYFQDGITNGAAWYNVHGGMQDWNYMNTNCFEVTIELGCHKYPPVADLQKYWEQNRKSLLQFIHQVHRGVKGMVIDSKDGSGIPNATITVETIHHQVTSSSLGDYWRLLVPGKYKLTASAQGYMSDSSSVTVPKDGLEIVNFTLTRIPTHANGQETENTTPVLDPSVREFQALIKQLSTQSGLDKLIQDTPTESSFRYQQYGEVSEFLRGLTLNFPEITSLRSLGQSVEIRNIWALEISNNPKVQEPSKPKIRFVAGIHGNAPVGTELLLEFAESLCVNYGKNAAINRLINETRIVILPSINPDGRELAKERDCTSTVGMTNVHGKDLDTDFIGNASQRVSEPQPETRAVMNLIQERGFTLSVALDGGSLLVTYPYDKPVQTVENDDTLRYLATVYADHHPTMHLGNTGCPNSVQSNIPGGVLRAAVWHSHMGSMKDFSVDFGHCPEITVYTSCCLFPSAEMLPSLWAENRKSLLSMLVEVHKGVRGIVKDKNGKPIVGAAIVLNGGVRVYTSEGGYFHALLAPGLHSIEAVADGYQQQSQKVSVSQFEAASSIIIEFDMENDIFGLPRELVVASAAAAMTALVLTACIIWCVCSAKSNRHKDGFHRLRQHRDDYDDEVRLMSMGSKKSLLSHEFQDESESDEDTLYTNKL

Target ORF information:

RefSeq Version	XM_686372.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_686372.8

ORF Insert Sequence:

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGGTAATG CATCTCAGCG AGTTTCAGAA 
CCTCAGCCAG AGACACGCGC TGTGATGAAT CTGATTCAGG AAAGAGGCTT CACTCTCTCT 
GTGGCTCTGG ATGGAGGCTC ATTGTTGGTC ACTTATCCAT ATGACAAACC AGTGCAAACC 
GTTGAAAATG ATGACACACT GAGATATCTG GCCACTGTGT ATGCTGATCA CCATCCCACA 
ATGCACTTGG GTAACACTGG ATGTCCAAAC AGCGTCCAAA GTAACATCCC TGGTGGCGTC 
CTTCGAGCAG CAGTGTGGCA CAGTCACATG GGCAGCATGA AGGACTTCAG TGTGGACTTT 
GGTCACTGTC CCGAGATCAC AGTCTACACC AGCTGCTGTC TGTTTCCTTC AGCTGAAATG 
CTGCCCTCGC TCTGGGCTGA GAACAGGAAG TCTCTTCTGA GCATGCTGGT TGAGGTGCAC 
AAAGGGGTTC GTGGAATAGT CAAGGATAAG AACGGTAAGC CCATCGTGGG TGCTGCAATT 
GTGTTGAACG GTGGTGTTCG AGTCTACACT AGTGAAGGAG GGTATTTCCA TGCTCTTCTG 
GCTCCTGGTT TACACAGCAT TGAAGCGGTG GCTGATGGAT ACCAACAGCA ATCACAGAAG 
GTGTCTGTGT CGCAATTTGA AGCAGCAAGT TCCATTATCA TTGAGTTTGA CATGGAGAAC 
GATATATTTG GCCTTCCCAG AGAATTAGTA GTGGCGAGTG CAGCTGCCGC AATGACGGCA 
CTAGTCTTGA CCGCCTGTAT CATCTGGTGC GTCTGCTCGG CCAAATCCAA CAGACATAAA 
GATGGCTTCC ATCGACTACG GCAGCATCGA GATGATTATG ACGACGAGGT CCGCCTCATG 
TCTATGGGCT CCAAAAAGTC TCTCCTCAGT CATGAGTTTC AAGATGAGAG CGAAAGTGAT 
GAGGATACAC TGTACACCAA CAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpda ( XM_686372.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_691464.3 Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.

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CloneID	ODa20528
Clone ID Related Accession (Same CDS sequence)	XM_021472947.1 , XM_021474349.1 , XM_686372.8 , XM_686372.9
Accession Version	XM_686372.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_686372.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGGTAATG CATCTCAGCG AGTTTCAGAA 
CCTCAGCCAG AGACACGCGC TGTGATGAAT CTGATTCAGG AAAGAGGCTT CACTCTCTCT 
GTGGCTCTGG ATGGAGGCTC ATTGTTGGTC ACTTATCCAT ATGACAAACC AGTGCAAACC 
GTTGAAAATG ATGACACACT GAGATATCTG GCCACTGTGT ATGCTGATCA CCATCCCACA 
ATGCACTTGG GTAACACTGG ATGTCCAAAC AGCGTCCAAA GTAACATCCC TGGTGGCGTC 
CTTCGAGCAG CAGTGTGGCA CAGTCACATG GGCAGCATGA AGGACTTCAG TGTGGACTTT 
GGTCACTGTC CCGAGATCAC AGTCTACACC AGCTGCTGTC TGTTTCCTTC AGCTGAAATG 
CTGCCCTCGC TCTGGGCTGA GAACAGGAAG TCTCTTCTGA GCATGCTGGT TGAGGTGCAC 
AAAGGGGTTC GTGGAATAGT CAAGGATAAG AACGGTAAGC CCATCGTGGG TGCTGCAATT 
GTGTTGAACG GTGGTGTTCG AGTCTACACT AGTGAAGGAG GGTATTTCCA TGCTCTTCTG 
GCTCCTGGTT TACACAGCAT TGAAGCGGTG GCTGATGGAT ACCAACAGCA ATCACAGAAG 
GTGTCTGTGT CGCAATTTGA AGCAGCAAGT TCCATTATCA TTGAGTTTGA CATGGAGAAC 
GATATATTTG GCCTTCCCAG AGAATTAGTA GTGGCGAGTG CAGCTGCCGC AATGACGGCA 
CTAGTCTTGA CCGCCTGTAT CATCTGGTGC GTCTGCTCGG CCAAATCCAA CAGACATAAA 
GATGGCTTCC ATCGACTACG GCAGCATCGA GATGATTATG ACGACGAGGT CCGCCTCATG 
TCTATGGGCT CCAAAAAGTC TCTCCTCAGT CATGAGTTTC AAGATGAGAG CGAAAGTGAT 
GAGGATACAC TGTACACCAA CAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_691464.3
CDS	126..4175
Translation	MSGQRARDLCSANKCPLLLLSLLVVYISSINSVSAGELKTETYNKYYNYDELTELLKSLAVKHPSIANLTSIGRSVEGRELWVMRVTKDISAADGTGKPKFKYVGNMHGDETVSRQVLVYLLEDLLEKYGEDQRVTELVNTTDIYILPSMNPDGFERSVEGDCLGKDEGRHNAKNIDLNRSFPDQFEEIHLNAEDIPEVTAVIKWILENKFVLSGNLHGGTVVASYPYDDSAGHSTDGTYSRSPDDALFRHLALVYAENNPVMKTGQPKCEDNVNESFPDGITNGAKWYDVPGGMQDFNYLKGNCLEITMELSCCKYPPSSQLKTEWENNRDALLAYMEQIHIGVRGFVRAAKNEAPIADAVIMVADIKHNVSTSRFGDYYRLLLPGTYSITAVAPGYIPMTVAGVEVKEGKATVLNITLLSENDERSTATPNPESSTDTSASSSNSGSTDNNSNSGASDKAADLTIQPQEFRHHRYIDMELFMKKFSSEYSSITRLYSIGKSVQKRLLWVMEISNNPGVHELGEPEFKYIGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVDTTRIHIMPSMNPDGYEVSQKGDVEGIKGRNNSKNYDLNRNFPDRFKLITDPIQPETLAVMNWSKNYSFVLSANLHGGSLVVNYPFDDNLEDVIGYSKSPDDAVFRMVALAYSQENSEMHEGHPCKEMSSYPEYFQDGITNGAAWYNVHGGMQDWNYMNTNCFEVTIELGCHKYPPVADLQKYWEQNRKSLLQFIHQVHRGVKGMVIDSKDGSGIPNATITVETIHHQVTSSSLGDYWRLLVPGKYKLTASAQGYMSDSSSVTVPKDGLEIVNFTLTRIPTHANGQETENTTPVLDPSVREFQALIKQLSTQSGLDKLIQDTPTESSFRYQQYGEVSEFLRGLTLNFPEITSLRSLGQSVEIRNIWALEISNNPKVQEPSKPKIRFVAGIHGNAPVGTELLLEFAESLCVNYGKNAAINRLINETRIVILPSINPDGRELAKERDCTSTVGMTNVHGKDLDTDFIGNASQRVSEPQPETRAVMNLIQERGFTLSVALDGGSLLVTYPYDKPVQTVENDDTLRYLATVYADHHPTMHLGNTGCPNSVQSNIPGGVLRAAVWHSHMGSMKDFSVDFGHCPEITVYTSCCLFPSAEMLPSLWAENRKSLLSMLVEVHKGVRGIVKDKNGKPIVGAAIVLNGGVRVYTSEGGYFHALLAPGLHSIEAVADGYQQQSQKVSVSQFEAASSIIIEFDMENDIFGLPRELVVASAAAAMTALVLTACIIWCVCSAKSNRHKDGFHRLRQHRDDYDDEVRLMSMGSKKSLLSHEFQDESESDEDTLYTNKL

Target ORF information:

RefSeq Version	XM_686372.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase D, a (cpda), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_686372.9

ORF Insert Sequence:

ATGTCGGGAC AGAGGGCAAG AGACCTGTGT TCAGCGAATA AATGCCCGCT GCTGCTTTTA 
TCTCTGCTCG TTGTTTATAT CAGCTCCATC AACTCAGTTT CCGCAGGCGA GCTGAAGACA 
GAGACTTACA ACAAATACTA CAATTACGAC GAACTTACCG AGCTTCTCAA AAGTCTCGCC 
GTTAAACACC CCAGCATCGC CAATCTGACC AGCATCGGCA GGTCCGTTGA AGGCAGAGAG 
CTGTGGGTGA TGAGAGTCAC CAAAGACATC AGTGCTGCTG ATGGAACAGG AAAACCCAAA 
TTCAAGTATG TGGGCAACAT GCACGGAGAC GAGACCGTGT CCCGACAGGT GTTGGTTTAC 
CTGCTGGAGG ACCTACTGGA GAAGTACGGG GAGGATCAGC GGGTCACGGA GCTCGTCAAC 
ACCACCGATA TTTACATCCT GCCGAGTATG AATCCTGACG GCTTTGAAAG GTCCGTGGAG 
GGGGATTGTT TGGGCAAAGA TGAGGGTCGG CATAATGCTA AAAACATCGA CCTGAATAGA 
AGTTTCCCAG ATCAGTTTGA GGAAATACAC TTGAATGCAG AAGACATCCC TGAAGTTACT 
GCTGTCATAA AGTGGATCCT GGAGAACAAA TTTGTTCTCT CTGGTAATCT GCACGGCGGG 
ACGGTAGTGG CCAGTTATCC CTATGATGAC TCTGCAGGTC ATTCTACTGA TGGCACCTAT 
AGCAGATCTC CCGATGATGC CCTCTTCAGG CACTTGGCCC TTGTTTACGC AGAGAATAAC 
CCGGTTATGA AAACCGGGCA GCCAAAGTGT GAGGACAACG TGAACGAATC CTTCCCGGAT 
GGCATCACAA ATGGAGCAAA GTGGTATGAC GTACCAGGGG GTATGCAGGA TTTCAATTAC 
CTGAAGGGGA ACTGTTTGGA AATCACCATG GAACTCAGCT GCTGCAAATA CCCACCCTCA 
TCCCAACTCA AGACCGAATG GGAAAACAAT CGTGACGCCC TACTGGCCTA CATGGAGCAG 
ATTCATATTG GAGTGCGTGG CTTTGTAAGG GCCGCCAAAA ATGAAGCTCC TATCGCTGAT 
GCTGTGATTA TGGTTGCGGA CATTAAACAC AATGTGAGCA CCTCACGTTT TGGAGACTAC 
TATCGTCTGT TGCTGCCTGG CACCTACAGC ATCACCGCGG TAGCACCGGG CTACATCCCT 
ATGACTGTAG CTGGGGTGGA GGTAAAAGAG GGAAAAGCAA CTGTGCTGAA TATCACCCTG 
TTGTCGGAGA ATGACGAGAG ATCCACAGCC ACCCCAAACC CAGAATCCTC CACTGACACC 
TCTGCCAGCA GCTCTAACAG TGGCAGCACA GACAACAACA GCAACTCTGG TGCCAGCGAC 
AAAGCAGCAG ATCTCACAAT ACAGCCTCAG GAGTTCCGGC ATCACCGGTA CATTGACATG 
GAGCTGTTCA TGAAGAAATT CAGCTCTGAG TACAGCTCCA TCACACGCCT CTATTCCATT 
GGGAAGTCTG TGCAGAAACG TCTCTTGTGG GTCATGGAGA TCTCCAACAA TCCTGGTGTC 
CACGAGTTAG GTGAACCGGA GTTTAAGTAT ATCGGTAACA TGCATGGCAA TGAAGTGGTT 
GGTCGTGAAT TGCTCCTCAA CCTCATTGAG TACCTGTGTC GCAACTATGG CACTGACCCT 
GAGGTCACCC AGCTTGTCGA CACCACACGG ATCCACATCA TGCCTTCAAT GAACCCGGAT 
GGTTATGAAG TGTCCCAAAA GGGAGATGTC GAAGGCATAA AGGGCCGTAA CAACAGTAAG 
AACTACGACC TGAACCGTAA CTTTCCTGAC CGGTTCAAAC TGATCACCGA TCCCATCCAG 
CCAGAGACCC TGGCTGTGAT GAACTGGTCA AAGAACTACT CATTTGTGTT GTCTGCCAAC 
CTTCATGGAG GCTCTCTGGT GGTGAATTAT CCATTTGACG ATAATCTTGA GGACGTTATC 
GGATACAGCA AGTCCCCAGA TGATGCTGTA TTCAGGATGG TGGCTCTCGC TTACTCTCAG 
GAAAACTCTG AAATGCATGA AGGTCACCCC TGTAAGGAAA TGAGTTCATA CCCGGAATAT 
TTTCAAGATG GCATCACCAA TGGCGCAGCA TGGTACAATG TCCACGGAGG AATGCAGGAC 
TGGAACTACA TGAACACAAA CTGCTTTGAG GTGACTATTG AACTGGGCTG TCACAAATAT 
CCGCCAGTTG CGGATCTGCA GAAATACTGG GAACAAAACC GCAAATCACT TCTGCAGTTC 
ATCCATCAGG TCCACCGTGG AGTAAAAGGA ATGGTAATCG ACAGCAAAGA TGGATCTGGC 
ATTCCCAATG CTACCATCAC TGTGGAAACC ATCCATCATC AAGTCACATC CAGTTCTTTA 
GGGGACTACT GGCGCTTACT CGTTCCAGGA AAATACAAGC TGACTGCCTC TGCCCAAGGC 
TACATGTCAG ATAGTTCTTC TGTCACCGTG CCTAAGGACG GATTGGAGAT TGTTAATTTC 
ACACTGACCC GGATTCCCAC ACATGCAAAC GGACAGGAGA CAGAAAACAC AACCCCAGTT 
TTGGACCCCA GTGTAAGGGA GTTTCAGGCC CTCATCAAGC AGCTGTCAAC CCAATCCGGT 
TTGGACAAAC TGATCCAGGA CACTCCTACC GAGAGTAGCT TCCGGTACCA GCAGTACGGC 
GAGGTTTCCG AGTTCCTGCG TGGACTGACA CTTAACTTTC CAGAGATTAC TTCTCTCCGC 
AGTCTTGGTC AGAGTGTGGA GATCAGGAAT ATCTGGGCTC TGGAGATCTC CAACAATCCT 
AAAGTCCAAG AACCGTCAAA GCCCAAGATC CGATTTGTAG CAGGAATCCA TGGAAACGCA 
CCAGTGGGCA CAGAGCTGCT TCTGGAGTTT GCTGAAAGTC TCTGTGTAAA CTACGGCAAG 
AATGCAGCCA TCAACAGGCT CATAAATGAG ACAAGAATTG TTATCCTGCC AAGCATCAAC 
CCAGATGGAC GTGAGCTGGC GAAGGAAAGA GACTGCACCT CCACTGTTGG CATGACCAAT 
GTGCATGGCA AAGACCTTGA CACTGACTTC ATTGGTAATG CATCTCAGCG AGTTTCAGAA 
CCTCAGCCAG AGACACGCGC TGTGATGAAT CTGATTCAGG AAAGAGGCTT CACTCTCTCT 
GTGGCTCTGG ATGGAGGCTC ATTGTTGGTC ACTTATCCAT ATGACAAACC AGTGCAAACC 
GTTGAAAATG ATGACACACT GAGATATCTG GCCACTGTGT ATGCTGATCA CCATCCCACA 
ATGCACTTGG GTAACACTGG ATGTCCAAAC AGCGTCCAAA GTAACATCCC TGGTGGCGTC 
CTTCGAGCAG CAGTGTGGCA CAGTCACATG GGCAGCATGA AGGACTTCAG TGTGGACTTT 
GGTCACTGTC CCGAGATCAC AGTCTACACC AGCTGCTGTC TGTTTCCTTC AGCTGAAATG 
CTGCCCTCGC TCTGGGCTGA GAACAGGAAG TCTCTTCTGA GCATGCTGGT TGAGGTGCAC 
AAAGGGGTTC GTGGAATAGT CAAGGATAAG AACGGTAAGC CCATCGTGGG TGCTGCAATT 
GTGTTGAACG GTGGTGTTCG AGTCTACACT AGTGAAGGAG GGTATTTCCA TGCTCTTCTG 
GCTCCTGGTT TACACAGCAT TGAAGCGGTG GCTGATGGAT ACCAACAGCA ATCACAGAAG 
GTGTCTGTGT CGCAATTTGA AGCAGCAAGT TCCATTATCA TTGAGTTTGA CATGGAGAAC 
GATATATTTG GCCTTCCCAG AGAATTAGTA GTGGCGAGTG CAGCTGCCGC AATGACGGCA 
CTAGTCTTGA CCGCCTGTAT CATCTGGTGC GTCTGCTCGG CCAAATCCAA CAGACATAAA 
GATGGCTTCC ATCGACTACG GCAGCATCGA GATGATTATG ACGACGAGGT CCGCCTCATG 
TCTATGGGCT CCAAAAAGTC TCTCCTCAGT CATGAGTTTC AAGATGAGAG CGAAAGTGAT 
GAGGATACAC TGTACACCAA CAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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cpda cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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