helq cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	helq
Entrez Gene ID	562952
Full Name	helicase, POLQ like
Synonyms	hel308,si:ch211-85e10.2
General protein information	Preferred Names helicase, POLQ like Names helicase POLQ-like helicase, mus308-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001282423.1	NP_001269352.1	helicase POLQ-like

Pathways from BioSystems

Homologies

Bos taurus (cattle)	HELQ	XP_002688448.3
Xenopus tropicalis (tropical clawed frog)	helq	XP_002939628.2
Canis lupus familiaris (dog)	HELQ	XP_544959.2
Homo sapiens (human)	HELQ	NP_598375.2
	HELQ	XP_001104832.1
Rattus norvegicus (Norway rat)	Helq	NP_001014156.2
Drosophila melanogaster (fruit fly)	mus301	NP_648178.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012297	XP_551895.3
Pan troglodytes (chimpanzee)	HELQ	XP_003310356.1
Mus musculus (house mouse)	Helq	NP_001074576.1
Gallus gallus (chicken)	HELQ	XP_420565.1
Danio rerio (zebrafish)	helq	XP_691411.3
Caenorhabditis elegans (roundworm)	helq-1	NP_001022911.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following helq gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the helq cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35749	NM_001282423.1 Latest version!	Danio rerio helicase, POLQ like (helq), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

helq ( NM_001282423.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001269352.1 Danio rerio helicase, POLQ like (helq), mRNA.

Price & Availability↑

CloneID	ODa35749
Clone ID Related Accession (Same CDS sequence)	NM_001282423.1
Accession Version	NM_001282423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	helicase POLQ-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX649525.15. On Sep 5, 2013 this sequence version replaced XM_686319.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01012424.1, GDQH01036375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTATACTG ACAACCAACT ACACGACATT GTGGTAAAGC GCAACACTTC GTCCTCAAGA 
AAGAGGTCAA GGGATGGAGT GAGAAGTCAC GTTACTCCTG CAAAGAAAAG GTCCAGTTTC 
ACATCAACGA CATGTTTAGC AGATAAAGAA CAGATTTATA CTCAAGACAT GGCGGAGAAT 
GAAGGAGTAG AGCTGTGCTG CAGTGACAAT GAAGATCTCT TTGAGGGTTA TGACAGCATA 
GTGGCTGACA GCTCTTTTCT GGCAAAGCTT GAAGATGTGG AGCTCCAGAC GAGGCAGTGC 
TATGATCAGC AAACTCCCAA TGCCTGTGCG GATGATCTCT CTGACTCCAT GTTAGCTGAG 
GATTTCAGAG ACTCGTCGCC GAGAGCTTTA CCAAGCTCTC AACTTGAATT TCAAAAAGCT 
ATCACAATGC CACACAAAAG CCCCTCCAGA GGTGCGCCGA GCTCTTCTAC CCCTTATTTG 
ATGAATCCTG GCCCGGCTGT CAATCATGCA GATCTCAGCG TCAACAAGCC TCATCCAAAG 
GCCAGAAGAA GCATGAAGGA CCATCTTAAG AAAGTGCTGA TGGATAATGC AGCAACGGCC 
AGCACAGTCT CCAAGATGGT ACAGCAAAAA GAAGCTGTGA TGAACGAAGA AATGAGTTTT 
GCCATGCAGG CTATGGAGTC CATAACATCT GAGGGAGACC TGGGTCCCTT TTTTGGCCTT 
CCGTCCAAAG TTAAAGATCT TATTTTTAGA TTAAAGGGAA TCCAGGACTT GTATGAGTGG 
CAGAAGACCT GTTTGAGCCT GGATTCAATT CAGCAGAGGA GAAATCTTAT TTATTCGCTA 
CCTACAAGTG GAGGAAAGAC TCTAGTGGCA GAGATTCTCA TATTTAAAGA GCTCTTGTGT 
AGAAAGAAAG ATGCACTACT TATTTTGCCG TACATATCCT TAGTTCAGGA AAAGGTTCGA 
GGGTTGTCTA GTTTTGGAAT TGAGTTGGAT TTCTTGGTGG AGGAGTATGC TGGTAGTAAA 
GGGAAGTTTC CTCCAGTCAA GAGAAGAAAT AAAAACTCTC TCTACATCAC AACCATTGAG 
AAGGGACACA GCCTTGTCAA TTCATTAATC GAAAATGACC GTTTAGACAA CATTGGCCTG 
GTTGTTGTGG ATGAGCTTCA CATGCTTGGT GATGGGAGCA GGGGGGCGAT CCTTGAAATG 
ACTTTGTCCA AGATTCTCTA CATGAGCAAA TCAACCCAAG TTATCGGCAT GAGCGCAACA 
CTAGGCAATG TGAAAGATCT TCAGAGCTTC CTGAGAGCTG AAAATTACAC CAATAACTTC 
AGGCCTGTTG AGCTGAAAGA ATATGTGAAA ATAAAGGACA GCATATATGA AGTTGACCCC 
AAGGAAGAGG CATGCTTCAC TTTCTCACGA CTCCTAAATT TTAAGTACTC CAGTGGAATG 
CAGAAAATGG ACCCGGACCA CATCATAGCT CTGGCTACTG AAGTCATCCC GCAGCAGTCC 
TGCCTCATAT TCTGTGCCAC CAAGAAGAAC TGTGAGAATC TAGCCGGAAT GATCTGCAAG 
TATTTAAATA AAGAGTTCAT AAAACACAAG GAGGCAGAAA AAGCCATTCT TCTGGGTGAG 
CTGAAGAGCA GTGGGAACGG CTCACTGTGT CCTGTCCTGC AGAAGACCAT ACCCTTCGGT 
TTGGCCTACC ATCACAGCGG CCTGACCAGC GATGAGAGGA AGCTGGTAGA AGAGGCTTAT 
TCCTCAGGAG TGCTCTGTCT CCTCACCTGC ACCTCTACTC TTGCTGCAGG CATCAATCTT 
CCTGCACGCA GAGTGATTCT GCGCTCCCCG TATGTGGCTG CAGACTTTTT AAAGAGAAGC 
CAGTACAAAC AGATGGTGGG CAGAGCAGGA CGAGCTGGAA TTGATGCCAT GGGCGAGAGC 
ATCCTGATCC TGCAGGACAA AGATATAAAC ACGGCCAAAA AACTACTGTG TGCTCCAATG 
GAGAAGTGTT ACAGTAACCT GCTGCACGAT GGAGGGCGGG GCCTGCTCAG CCTCATTCTG 
TCTCTCATTG GCCTGAAAAT AACCACAACT GTAGAGCAGG TGAAGGATTT GATGAAAGGC 
ACGTTTCTCG GTGTGCAGGA GGCTCAGGTC AGCCCAGAGA AGAGCTTGTG GGATCTTACA 
GTGGAGTCCA TACAGACACT GAAGCAGAAG AACCTCATCG AAGTCTCTTC TGATGAAAAT 
AATCAAAACA TTCTGCAAAT CACCAGGCTT GGGCGAGCCA CTTATAAAGG TTCAGTAGAC 
CTAAGCTACT GTGATGTGCT CTACCGAGAC CTTTCAAAAG GGCTGGAGGG TTTACTCCTC 
AACAGTTTTC TTCACTTAGT GTATCTAGTT ACTCCATATG ACATGGTGCA TCAGTGCAAA 
CCGGATTGGA TGATCTACTT CAGGCAGTTC ACAAATTTGT CAGCTGCTGA GCAGAAGATG 
GCCACCACGG TCGGAGTTCC TGAAAGCTTT GTGGCCAGAA AGGCGGCAGG ACAGAGCGTG 
AGGAAGTCTG TGGATGTTGT GGTAGTGAAC AGGTTGTACC TGGCTCTGGT CCTGTTCTCC 
TTACTGAAGG AAACAAACCT GTGGATTGTG TCCGAACGAT TCCAGCTGAC CAGAGGTTTT 
GTTCAGACTC TTCTCAGCTC AGCGTCTGCC TTTGGATCAT CTGTGCTGCA CTTCACGGAG 
GAGCTGGAGG AGTTTTGGGC ATACAAAGCT CTTCTCTCTG AGTTGACCCG AAGATTGACT 
TACTGTGTGC AGGCCGAGCT CATTCCTCTC ATGGAGGTGT CTGGAGTCCT GGAGCATCGT 
GCTAAGCAGC TGTACAGTGC CGGATACAAG ACATTATCAC ATCTGGCCAA TGCAGATCCA 
CAAACTCTGG TCCAAACTAT TGAAAACCTC TTTAAGAAAC AAGCGTATCA AATTATTGCA 
TCTGCAAAAA TGTTATTAAC AGAAAAAGCA GAAGCCCTGC AGGAGGAGGT GGACGACCTT 
CTTATGCTGC CATTGGATCT CCCATCATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269352.1
CDS	280..3312
Translation	MYTDNQLHDIVVKRNTSSSRKRSRDGVRSHVTPAKKRSSFTSTTCLADKEQIYTQDMAENEGVELCCSDNEDLFEGYDSIVADSSFLAKLEDVELQTRQCYDQQTPNACADDLSDSMLAEDFRDSSPRALPSSQLEFQKAITMPHKSPSRGAPSSSTPYLMNPGPAVNHADLSVNKPHPKARRSMKDHLKKVLMDNAATASTVSKMVQQKEAVMNEEMSFAMQAMESITSEGDLGPFFGLPSKVKDLIFRLKGIQDLYEWQKTCLSLDSIQQRRNLIYSLPTSGGKTLVAEILIFKELLCRKKDALLILPYISLVQEKVRGLSSFGIELDFLVEEYAGSKGKFPPVKRRNKNSLYITTIEKGHSLVNSLIENDRLDNIGLVVVDELHMLGDGSRGAILEMTLSKILYMSKSTQVIGMSATLGNVKDLQSFLRAENYTNNFRPVELKEYVKIKDSIYEVDPKEEACFTFSRLLNFKYSSGMQKMDPDHIIALATEVIPQQSCLIFCATKKNCENLAGMICKYLNKEFIKHKEAEKAILLGELKSSGNGSLCPVLQKTIPFGLAYHHSGLTSDERKLVEEAYSSGVLCLLTCTSTLAAGINLPARRVILRSPYVAADFLKRSQYKQMVGRAGRAGIDAMGESILILQDKDINTAKKLLCAPMEKCYSNLLHDGGRGLLSLILSLIGLKITTTVEQVKDLMKGTFLGVQEAQVSPEKSLWDLTVESIQTLKQKNLIEVSSDENNQNILQITRLGRATYKGSVDLSYCDVLYRDLSKGLEGLLLNSFLHLVYLVTPYDMVHQCKPDWMIYFRQFTNLSAAEQKMATTVGVPESFVARKAAGQSVRKSVDVVVVNRLYLALVLFSLLKETNLWIVSERFQLTRGFVQTLLSSASAFGSSVLHFTEELEEFWAYKALLSELTRRLTYCVQAELIPLMEVSGVLEHRAKQLYSAGYKTLSHLANADPQTLVQTIENLFKKQAYQIIASAKMLLTEKAEALQEEVDDLLMLPLDLPSL

Target ORF information:

RefSeq Version	NM_001282423.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio helicase, POLQ like (helq), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282423.1

ORF Insert Sequence:

ATGTATACTG ACAACCAACT ACACGACATT GTGGTAAAGC GCAACACTTC GTCCTCAAGA 
AAGAGGTCAA GGGATGGAGT GAGAAGTCAC GTTACTCCTG CAAAGAAAAG GTCCAGTTTC 
ACATCAACGA CATGTTTAGC AGATAAAGAA CAGATTTATA CTCAAGACAT GGCGGAGAAT 
GAAGGAGTAG AGCTGTGCTG CAGTGACAAT GAAGATCTCT TTGAGGGTTA TGACAGCATA 
GTGGCTGACA GCTCTTTTCT GGCAAAGCTT GAAGATGTGG AGCTCCAGAC GAGGCAGTGC 
TATGATCAGC AAACTCCCAA TGCCTGTGCG GATGATCTCT CTGACTCCAT GTTAGCTGAG 
GATTTCAGAG ACTCGTCGCC GAGAGCTTTA CCAAGCTCTC AACTTGAATT TCAAAAAGCT 
ATCACAATGC CACACAAAAG CCCCTCCAGA GGTGCGCCGA GCTCTTCTAC CCCTTATTTG 
ATGAATCCTG GCCCGGCTGT CAATCATGCA GATCTCAGCG TCAACAAGCC TCATCCAAAG 
GCCAGAAGAA GCATGAAGGA CCATCTTAAG AAAGTGCTGA TGGATAATGC AGCAACGGCC 
AGCACAGTCT CCAAGATGGT ACAGCAAAAA GAAGCTGTGA TGAACGAAGA AATGAGTTTT 
GCCATGCAGG CTATGGAGTC CATAACATCT GAGGGAGACC TGGGTCCCTT TTTTGGCCTT 
CCGTCCAAAG TTAAAGATCT TATTTTTAGA TTAAAGGGAA TCCAGGACTT GTATGAGTGG 
CAGAAGACCT GTTTGAGCCT GGATTCAATT CAGCAGAGGA GAAATCTTAT TTATTCGCTA 
CCTACAAGTG GAGGAAAGAC TCTAGTGGCA GAGATTCTCA TATTTAAAGA GCTCTTGTGT 
AGAAAGAAAG ATGCACTACT TATTTTGCCG TACATATCCT TAGTTCAGGA AAAGGTTCGA 
GGGTTGTCTA GTTTTGGAAT TGAGTTGGAT TTCTTGGTGG AGGAGTATGC TGGTAGTAAA 
GGGAAGTTTC CTCCAGTCAA GAGAAGAAAT AAAAACTCTC TCTACATCAC AACCATTGAG 
AAGGGACACA GCCTTGTCAA TTCATTAATC GAAAATGACC GTTTAGACAA CATTGGCCTG 
GTTGTTGTGG ATGAGCTTCA CATGCTTGGT GATGGGAGCA GGGGGGCGAT CCTTGAAATG 
ACTTTGTCCA AGATTCTCTA CATGAGCAAA TCAACCCAAG TTATCGGCAT GAGCGCAACA 
CTAGGCAATG TGAAAGATCT TCAGAGCTTC CTGAGAGCTG AAAATTACAC CAATAACTTC 
AGGCCTGTTG AGCTGAAAGA ATATGTGAAA ATAAAGGACA GCATATATGA AGTTGACCCC 
AAGGAAGAGG CATGCTTCAC TTTCTCACGA CTCCTAAATT TTAAGTACTC CAGTGGAATG 
CAGAAAATGG ACCCGGACCA CATCATAGCT CTGGCTACTG AAGTCATCCC GCAGCAGTCC 
TGCCTCATAT TCTGTGCCAC CAAGAAGAAC TGTGAGAATC TAGCCGGAAT GATCTGCAAG 
TATTTAAATA AAGAGTTCAT AAAACACAAG GAGGCAGAAA AAGCCATTCT TCTGGGTGAG 
CTGAAGAGCA GTGGGAACGG CTCACTGTGT CCTGTCCTGC AGAAGACCAT ACCCTTCGGT 
TTGGCCTACC ATCACAGCGG CCTGACCAGC GATGAGAGGA AGCTGGTAGA AGAGGCTTAT 
TCCTCAGGAG TGCTCTGTCT CCTCACCTGC ACCTCTACTC TTGCTGCAGG CATCAATCTT 
CCTGCACGCA GAGTGATTCT GCGCTCCCCG TATGTGGCTG CAGACTTTTT AAAGAGAAGC 
CAGTACAAAC AGATGGTGGG CAGAGCAGGA CGAGCTGGAA TTGATGCCAT GGGCGAGAGC 
ATCCTGATCC TGCAGGACAA AGATATAAAC ACGGCCAAAA AACTACTGTG TGCTCCAATG 
GAGAAGTGTT ACAGTAACCT GCTGCACGAT GGAGGGCGGG GCCTGCTCAG CCTCATTCTG 
TCTCTCATTG GCCTGAAAAT AACCACAACT GTAGAGCAGG TGAAGGATTT GATGAAAGGC 
ACGTTTCTCG GTGTGCAGGA GGCTCAGGTC AGCCCAGAGA AGAGCTTGTG GGATCTTACA 
GTGGAGTCCA TACAGACACT GAAGCAGAAG AACCTCATCG AAGTCTCTTC TGATGAAAAT 
AATCAAAACA TTCTGCAAAT CACCAGGCTT GGGCGAGCCA CTTATAAAGG TTCAGTAGAC 
CTAAGCTACT GTGATGTGCT CTACCGAGAC CTTTCAAAAG GGCTGGAGGG TTTACTCCTC 
AACAGTTTTC TTCACTTAGT GTATCTAGTT ACTCCATATG ACATGGTGCA TCAGTGCAAA 
CCGGATTGGA TGATCTACTT CAGGCAGTTC ACAAATTTGT CAGCTGCTGA GCAGAAGATG 
GCCACCACGG TCGGAGTTCC TGAAAGCTTT GTGGCCAGAA AGGCGGCAGG ACAGAGCGTG 
AGGAAGTCTG TGGATGTTGT GGTAGTGAAC AGGTTGTACC TGGCTCTGGT CCTGTTCTCC 
TTACTGAAGG AAACAAACCT GTGGATTGTG TCCGAACGAT TCCAGCTGAC CAGAGGTTTT 
GTTCAGACTC TTCTCAGCTC AGCGTCTGCC TTTGGATCAT CTGTGCTGCA CTTCACGGAG 
GAGCTGGAGG AGTTTTGGGC ATACAAAGCT CTTCTCTCTG AGTTGACCCG AAGATTGACT 
TACTGTGTGC AGGCCGAGCT CATTCCTCTC ATGGAGGTGT CTGGAGTCCT GGAGCATCGT 
GCTAAGCAGC TGTACAGTGC CGGATACAAG ACATTATCAC ATCTGGCCAA TGCAGATCCA 
CAAACTCTGG TCCAAACTAT TGAAAACCTC TTTAAGAAAC AAGCGTATCA AATTATTGCA 
TCTGCAAAAA TGTTATTAAC AGAAAAAGCA GAAGCCCTGC AGGAGGAGGT GGACGACCTT 
CTTATGCTGC CATTGGATCT CCCATCATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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helq cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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