cacna1bb cDNA ORF clone, Danio rerio(zebrafish)

The following cacna1bb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1bb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa56945 XM_017352996.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa56944 XM_017352995.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa146096 XM_021473395.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa146097 XM_021473396.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa146098 XM_021473397.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa146099 XM_021473398.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa151030 XM_021469036.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa151031 XM_017352992.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa151032 XM_017352993.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa151033 XM_017352994.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa56940 XM_017352991.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa56941 XM_017352992.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa56942 XM_017352993.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa56943 XM_017352994.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa56945
    Clone ID Related Accession (Same CDS sequence) XM_017352996.1
    Accession Version XM_017352996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC
    ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC
    TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC
    ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT
    CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG
    GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT
    GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC
    TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA
    TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC
    TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG
    CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC
    AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC
    ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT
    CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA
    TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG
    GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA
    CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT
    CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT
    TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT
    GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA
    GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC
    ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC
    GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC
    TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG
    AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC
    CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC
    AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC
    AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA
    GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC
    TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT
    TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC
    ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC
    TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG
    ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG
    CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG
    CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT
    GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC
    TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC
    GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA
    GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA
    GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208485.1
    CDS180..4004
    Translation

    Target ORF information:

    RefSeq Version XM_017352996.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC
    ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC
    TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC
    ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT
    CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG
    GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT
    GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC
    TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA
    TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC
    TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG
    CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC
    AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC
    ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT
    CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA
    TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG
    GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA
    CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT
    CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT
    TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT
    GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA
    GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC
    ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC
    GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC
    TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG
    AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC
    CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC
    AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC
    AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA
    GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC
    TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT
    TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC
    ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC
    TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG
    ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG
    CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG
    CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT
    GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC
    TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC
    GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA
    GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA
    GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56944
    Clone ID Related Accession (Same CDS sequence) XM_017352995.1
    Accession Version XM_017352995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTGCAGTT TTGTAAAGCA AAATCGAGAT GCAATCTCTC GCAGGTCTTC AGTCTGCAGC 
    GTTCATTCAC CTAAAGAGCA GCAGAGGTCA CTGCAGAAGT TGTCCATATG GGAGCAGCGC
    ACTAACCAAC TGCGCAGACA CAACCTGCGT AGCAGCAGCG AGGCACTTTA CAACGAATTG
    GAGCCAGAAG AGCGTCTGCG TGTCTCCTCT GCCCTGCATC TGCGGCCTGA CATGAAGACC
    CACCACGATC GGCCGCTGGT TGTGGAGCAC CGAGATTCTG TGGACAATCT CAGATCTGAC
    AAAGACGGAG ACACTTCAGA GGGTCAGCCA GCATCTAACC ACCAGAGAAA ATATCATAAA
    TGTCGAGGGG AGAACGGAGA CACCGGCGAG GGACGGCTTC ACAGACACCA CAGCCAAAAT
    CGAGAGAACC AGGGTGGCCT AGAGCGCAGC AACAGTCAAG ATGGAGGACA GAGGAACCAC
    CACAGATCTT CTGGATCTCC AGAGGAGGGC ATTGGGATTG AGGAGAGAGA ACATAGACAT
    CATCATTCTA GAAGACCCAG AGAAGTCAAC GGGAACGGGA ATGGTGTGAT TGGTAATGGA
    GGCAGGGGCG AGAGGATACG AGGTCACAGA GAGGGTGAAG GAGGGAATGG AGAGAGAAGG
    AGACACCGGC CACGGGTTAA AGCCCAGTCC ACATTGGATG GAGACGAATG TAGAGAGAAT
    GGTGGTAAAC GCAGGAGCAG ACCAACAGAA ACAGACAGCC TCACAACCAG CCCTCTCCAG
    TCTCCTTCTG AAGAGAAGCC AGAAGACAAA GATAATCTAA AAAACAGCAC TCGAGTGGGT
    CCAAGCGGAG TCAACATCCC TGTCACCATA ACTGCGCCAC CAGGAGAGAC CACTGTCATT
    CCAATGAACA ACATTGATGG TGACAGTCTG CTGCTGAATG AGGAAAAGAA GGATCTTGAT
    GAGCTAAATC AGAATGCGCC TAAACACATT TTGCCATACA GCTCCATGTT TGTGTTTGGC
    CCAACCAACC CAGTGCGACG ACTGTGTCAT TATGTGGTGA ATCTGCGGTA TTTTGAGATG
    TGCATCCTCA CAGTCATCAC CATGAGCAGT ATCGCTCTCG CTGCCGAAGA CCCCGTGCAG
    GCTCATGCCC CTCGCAATGA TGTGCTGAAA TACCTTGATT ACGTCTTCAC TGGTGTGTTC
    ACATTTGAGA TGGTTATAAA GATGATTGAT CTTGGACTGC TGCTGCATCC CGGCTCTTAC
    TTCAGGGACC TGTGGAACAT CCTGGACTTC ATCGTAGTGA GCGGCGCTCT GGTGGCTTTC
    GCCTGCTCGA GCTTCATGGG AGGAACTAAA GGAAAGGACA TAAACACCAT CAAGTCCCTG
    AGGGTTCTTC GAGTCTTGAG ACCCTTGAAG ACAATCAAAA GATTGCCCAA ACTCAAGGCC
    GTGTTTGACT GCGTGGTGAA CTCTCTGAAG AATGTGCTCA ACATCCTCAT CGTCTACATC
    CTCTTCATGT TCATCTTCGC GGTCATCGCC GTGCAGCTTT TCAAGGGGAA GTTTTTCCAC
    TGCACCGATG AGTCCAAAGC CCTGGAGAAG GACTGCAGGG GTCAGTTCTT GGAGTACGGC
    AGTGATGGAG TGGCCATGAC GCAGCCGCGC GAGTGGAAGA AGTACGACTT CCATTATGAC
    AACGTCCTGT GGGCCTTTCT GACCCTCTTC ACTGTGTCTA CAGGAGAGGG CTGGCCACTT
    GTGCTGAAAC ACTCTGTTGA TGCCACGTAT GAAGACCAGG GTCCCAGTCC AGGATATCGC
    ATGGAAACCT CCATCTTCTA TGTGGTCTAC TTCATCGTCT TTCCCTTCTT CTTTGTCAAC
    ATCTTTGTGG CTTTGATCAT CATCACATTC CAGGAGCAGG GGGATAAAGC CATGTCCGAG
    TGCAGCTTGG AAAAGAACGA GCGAGCTTGT ATTGACTTTG CCATCAATGC TAAACCTCTG
    ACTCGCTACA TGCCGCAAGA CAAGAACTCT TACCAGTACA AGATGTGGAA GTTTGTGGTG
    TCGACTCCGT TTGAGTACTC CATCCTGACC ATGATCGCCA TCAATACAGT GGTGCTCATG
    ATGAAGTTCC ATGGTGCTCC TAAACCTTAT GAGGACATGT TGAAGTGGCT GAACATCATC
    TTCACTGCAC TCTTCACACT GGAGTGTGTT CTCAAGGTCA TCGCCTTCGG TCCTCTGAAC
    TACCTGAAAG AGGCTTGGAA CATTTTTGAC TTTGTTACTG TTCTGGGAAG CATCACAGAC
    ATCCTGGTTA CTGAGATCAA GACGGATAAG AGGATTAATC TGAGTTTCCT GAGACTCTTC
    CGGGCGGCTC GTCTCATTAA ACTCCTTCGG CAAGGATACA CCATTCGGAT CCTTCTCTGG
    ACCTTTGTTC AGTCCTTTAA GGCTCTTCCA TATGTGTGTC TCCTTATCGC AATGCTTTTT
    TTCATTTACG CCATCATCGG GATGCAGGTG TTTGGTAACA TCGCGTTGAA CAATGAAGAT
    TCGGCCATCA ACATTCACAA TAACTTCCGC ACCTTTTTTC AGGCCCTCAT GTTGTTGTTC
    AGAAGTGCTA CAGGGGAGGC TTGGCACGAG ATCATGTTGG CTTGTCTGAG TGAAAGATAT
    TGCGATATAG AATCTGGCTA CAGTGGGAAA GAGTGCGGCA GCGATTTTGC ATACTTCTAC
    TTCGTCTCCT TCATCTTTCT TTGCTCGTTT CTGATGCTGA ATCTGTTTGT GGCTGTCATC
    ATGGATAATT TTGAATATCT GACACGAGAT GCTTCTATTC TGGGGCCTCA TCACCTAGAT
    GAGTTCATCC GCGTCTGGGC TGAGTACGAC CCTGCCGCCT GTGGACGCAT GTCCTACCTC
    GATATGTACG AGATGCTCCG CCACATGTCC CCTCCGCTAG GTTTGGGAAA GAAATGTCCT
    CCACGAATCG CCTACAAGCG ATTGGTGAGG ATGAACATGC CCATAGCCGA GGACAACACG
    GTTCACTTTA CCTCCACTTT AATGGCTCTC ATCCGGACTG CCCTGGAGAT CAAACTGGCA
    TCAGGAGTTC TGGCTCAGCG CATTTGTGAT GCTGAATTAA GGAAGGAAAT CAATAAAATT
    TGGCCCAGTT TGTCATCTAA AACAGTAGAC CTGCTGGTTA CACCTCATAA ACATAACGAA
    TTGACTGTCG GGAAGGTCTA TGCAGCGCTC ATGATTTTTG ACTACTATAA GCAGAACAGA
    GCCAAAAGAC TCCAACAGCA GCAAGCTGCA CCAGGCACAC AGAGTAAAGT CGGTGTTCTC
    TTCAGGCCGA TGCTTCCTCT CACACACCTG CATGACCAGG AGCCTTTAGT GCCAAAAGGC
    CCGCCTCCTT CTCATTTAGA TATCACCCCT CAGTCAGATG CCACACCCAC TTCTTTTCAC
    TTGACCAATG GGGATGCAGT TCAAAGCCAG AGCAGTGCCA TGAAAAAGTC TCCTTCGGGA
    GGCCCATCTC AGGAAGTCAC ACAGGAAACG AACAGAAGGC CTATCCAACG TGGTCAATCG
    GAAGACGTGC CAAACACTTA CAGATCTCAG GAACTAGTAG AGATGACTGA CATGGAGAAT
    GATTCGGATG TTGGCGGGTA TCATGTGCTG GAGGGTCATG GCAGAGCTGC CTCTATGCCC
    AGACTGAATG CTGGCTTCCA GCGGAGCCAC TTACGCCACA CCGCCGGAGC GTATCTAGCA
    CCAATTGCAG ATGTGAGTCC CATGCGGCGA TCCACATCCT CGCTGACTCC ACAAAGAGGT
    GCGCGAGAGG TCAACCTGAG CGAATACGAC CTGGAGAGAC CCAGGATGAG CCCGAGCCAG
    ACTTTCGCTC AGGACAGAGA GAGAGAGAGA GTCCACCATC ATCATCACCA CCATCATCAT
    CGCTGTCAGA GACGCAGAGA CAAGAAGCAC AAGTCACTGG ATCGAGCCAT CAGCGAGGAG
    CAGCCGCCAC CTGCAGCTGA TCCAAACACA GAGTCGACGC CCAGGGAACG AGCCCGGGAG
    CGAGACCGTG GCCGCTCACA CGAGAGGAAG CACCACTCAT CCTCAGCAGC AGAAAGGAAG
    CAGCGATACT ACTCTTGTGA CCGCTACGGC AGCCATGAAC ATGGCCACAG CCTGTCCGCC
    GGGCCTAGCC GATCCACCTC TCCTGGAGAA CCACATGACA GGCTTAAACA GGGAATTAAC
    GCAGCTAAAG GAAGCTCAGT GGGTCATACC TCAGGCACCA GCACACCCTG CCGCGGGCGT
    AGACAGCTTC CCCAGACACC TCTTACCCCT CGGCCTGCTG TTGCCTATAA GACTGCCAAC
    TCTTCTCCGG TCCAGCTCAA CACTGCCCAA TCTACAGGGC CCTGCCCCGC TCGCCTTAGT
    CGTGGCCTGT CAGAGCACGA TACCTTACTC AGCGGTAGCA TCCACCGCCA ATCCCCTGTG
    CCTGTCACTC GTATTGGCTC TGACCCCAGC TTAGGTCCCC TCCAGCAAGA CTCGCACTTG
    TCTGAAGCGG AAGACTTTCA CGATGCCTTG AGCACGTACG GCACCGGTCG CTCCCCTAGA
    ACTACCGCCA TCATCGCCCA CACGTCATCT GGTGCGACCG CCACTTCTAC GCTGCTGTCC
    CGAAGCCAGA GCGGAGTCCC CAACGGGTAC CACTACTCCC TCGGAGTGAA CGCGGCTCCG
    GGATTGAGTG GGAGAGCGTC TGGAAGCTAC CAGGAGACTG AGAGAGATGA CTGGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208484.1
    CDS52..4791
    Translation

    Target ORF information:

    RefSeq Version XM_017352995.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTGCAGTT TTGTAAAGCA AAATCGAGAT GCAATCTCTC GCAGGTCTTC AGTCTGCAGC 
    GTTCATTCAC CTAAAGAGCA GCAGAGGTCA CTGCAGAAGT TGTCCATATG GGAGCAGCGC
    ACTAACCAAC TGCGCAGACA CAACCTGCGT AGCAGCAGCG AGGCACTTTA CAACGAATTG
    GAGCCAGAAG AGCGTCTGCG TGTCTCCTCT GCCCTGCATC TGCGGCCTGA CATGAAGACC
    CACCACGATC GGCCGCTGGT TGTGGAGCAC CGAGATTCTG TGGACAATCT CAGATCTGAC
    AAAGACGGAG ACACTTCAGA GGGTCAGCCA GCATCTAACC ACCAGAGAAA ATATCATAAA
    TGTCGAGGGG AGAACGGAGA CACCGGCGAG GGACGGCTTC ACAGACACCA CAGCCAAAAT
    CGAGAGAACC AGGGTGGCCT AGAGCGCAGC AACAGTCAAG ATGGAGGACA GAGGAACCAC
    CACAGATCTT CTGGATCTCC AGAGGAGGGC ATTGGGATTG AGGAGAGAGA ACATAGACAT
    CATCATTCTA GAAGACCCAG AGAAGTCAAC GGGAACGGGA ATGGTGTGAT TGGTAATGGA
    GGCAGGGGCG AGAGGATACG AGGTCACAGA GAGGGTGAAG GAGGGAATGG AGAGAGAAGG
    AGACACCGGC CACGGGTTAA AGCCCAGTCC ACATTGGATG GAGACGAATG TAGAGAGAAT
    GGTGGTAAAC GCAGGAGCAG ACCAACAGAA ACAGACAGCC TCACAACCAG CCCTCTCCAG
    TCTCCTTCTG AAGAGAAGCC AGAAGACAAA GATAATCTAA AAAACAGCAC TCGAGTGGGT
    CCAAGCGGAG TCAACATCCC TGTCACCATA ACTGCGCCAC CAGGAGAGAC CACTGTCATT
    CCAATGAACA ACATTGATGG TGACAGTCTG CTGCTGAATG AGGAAAAGAA GGATCTTGAT
    GAGCTAAATC AGAATGCGCC TAAACACATT TTGCCATACA GCTCCATGTT TGTGTTTGGC
    CCAACCAACC CAGTGCGACG ACTGTGTCAT TATGTGGTGA ATCTGCGGTA TTTTGAGATG
    TGCATCCTCA CAGTCATCAC CATGAGCAGT ATCGCTCTCG CTGCCGAAGA CCCCGTGCAG
    GCTCATGCCC CTCGCAATGA TGTGCTGAAA TACCTTGATT ACGTCTTCAC TGGTGTGTTC
    ACATTTGAGA TGGTTATAAA GATGATTGAT CTTGGACTGC TGCTGCATCC CGGCTCTTAC
    TTCAGGGACC TGTGGAACAT CCTGGACTTC ATCGTAGTGA GCGGCGCTCT GGTGGCTTTC
    GCCTGCTCGA GCTTCATGGG AGGAACTAAA GGAAAGGACA TAAACACCAT CAAGTCCCTG
    AGGGTTCTTC GAGTCTTGAG ACCCTTGAAG ACAATCAAAA GATTGCCCAA ACTCAAGGCC
    GTGTTTGACT GCGTGGTGAA CTCTCTGAAG AATGTGCTCA ACATCCTCAT CGTCTACATC
    CTCTTCATGT TCATCTTCGC GGTCATCGCC GTGCAGCTTT TCAAGGGGAA GTTTTTCCAC
    TGCACCGATG AGTCCAAAGC CCTGGAGAAG GACTGCAGGG GTCAGTTCTT GGAGTACGGC
    AGTGATGGAG TGGCCATGAC GCAGCCGCGC GAGTGGAAGA AGTACGACTT CCATTATGAC
    AACGTCCTGT GGGCCTTTCT GACCCTCTTC ACTGTGTCTA CAGGAGAGGG CTGGCCACTT
    GTGCTGAAAC ACTCTGTTGA TGCCACGTAT GAAGACCAGG GTCCCAGTCC AGGATATCGC
    ATGGAAACCT CCATCTTCTA TGTGGTCTAC TTCATCGTCT TTCCCTTCTT CTTTGTCAAC
    ATCTTTGTGG CTTTGATCAT CATCACATTC CAGGAGCAGG GGGATAAAGC CATGTCCGAG
    TGCAGCTTGG AAAAGAACGA GCGAGCTTGT ATTGACTTTG CCATCAATGC TAAACCTCTG
    ACTCGCTACA TGCCGCAAGA CAAGAACTCT TACCAGTACA AGATGTGGAA GTTTGTGGTG
    TCGACTCCGT TTGAGTACTC CATCCTGACC ATGATCGCCA TCAATACAGT GGTGCTCATG
    ATGAAGTTCC ATGGTGCTCC TAAACCTTAT GAGGACATGT TGAAGTGGCT GAACATCATC
    TTCACTGCAC TCTTCACACT GGAGTGTGTT CTCAAGGTCA TCGCCTTCGG TCCTCTGAAC
    TACCTGAAAG AGGCTTGGAA CATTTTTGAC TTTGTTACTG TTCTGGGAAG CATCACAGAC
    ATCCTGGTTA CTGAGATCAA GACGGATAAG AGGATTAATC TGAGTTTCCT GAGACTCTTC
    CGGGCGGCTC GTCTCATTAA ACTCCTTCGG CAAGGATACA CCATTCGGAT CCTTCTCTGG
    ACCTTTGTTC AGTCCTTTAA GGCTCTTCCA TATGTGTGTC TCCTTATCGC AATGCTTTTT
    TTCATTTACG CCATCATCGG GATGCAGGTG TTTGGTAACA TCGCGTTGAA CAATGAAGAT
    TCGGCCATCA ACATTCACAA TAACTTCCGC ACCTTTTTTC AGGCCCTCAT GTTGTTGTTC
    AGAAGTGCTA CAGGGGAGGC TTGGCACGAG ATCATGTTGG CTTGTCTGAG TGAAAGATAT
    TGCGATATAG AATCTGGCTA CAGTGGGAAA GAGTGCGGCA GCGATTTTGC ATACTTCTAC
    TTCGTCTCCT TCATCTTTCT TTGCTCGTTT CTGATGCTGA ATCTGTTTGT GGCTGTCATC
    ATGGATAATT TTGAATATCT GACACGAGAT GCTTCTATTC TGGGGCCTCA TCACCTAGAT
    GAGTTCATCC GCGTCTGGGC TGAGTACGAC CCTGCCGCCT GTGGACGCAT GTCCTACCTC
    GATATGTACG AGATGCTCCG CCACATGTCC CCTCCGCTAG GTTTGGGAAA GAAATGTCCT
    CCACGAATCG CCTACAAGCG ATTGGTGAGG ATGAACATGC CCATAGCCGA GGACAACACG
    GTTCACTTTA CCTCCACTTT AATGGCTCTC ATCCGGACTG CCCTGGAGAT CAAACTGGCA
    TCAGGAGTTC TGGCTCAGCG CATTTGTGAT GCTGAATTAA GGAAGGAAAT CAATAAAATT
    TGGCCCAGTT TGTCATCTAA AACAGTAGAC CTGCTGGTTA CACCTCATAA ACATAACGAA
    TTGACTGTCG GGAAGGTCTA TGCAGCGCTC ATGATTTTTG ACTACTATAA GCAGAACAGA
    GCCAAAAGAC TCCAACAGCA GCAAGCTGCA CCAGGCACAC AGAGTAAAGT CGGTGTTCTC
    TTCAGGCCGA TGCTTCCTCT CACACACCTG CATGACCAGG AGCCTTTAGT GCCAAAAGGC
    CCGCCTCCTT CTCATTTAGA TATCACCCCT CAGTCAGATG CCACACCCAC TTCTTTTCAC
    TTGACCAATG GGGATGCAGT TCAAAGCCAG AGCAGTGCCA TGAAAAAGTC TCCTTCGGGA
    GGCCCATCTC AGGAAGTCAC ACAGGAAACG AACAGAAGGC CTATCCAACG TGGTCAATCG
    GAAGACGTGC CAAACACTTA CAGATCTCAG GAACTAGTAG AGATGACTGA CATGGAGAAT
    GATTCGGATG TTGGCGGGTA TCATGTGCTG GAGGGTCATG GCAGAGCTGC CTCTATGCCC
    AGACTGAATG CTGGCTTCCA GCGGAGCCAC TTACGCCACA CCGCCGGAGC GTATCTAGCA
    CCAATTGCAG ATGTGAGTCC CATGCGGCGA TCCACATCCT CGCTGACTCC ACAAAGAGGT
    GCGCGAGAGG TCAACCTGAG CGAATACGAC CTGGAGAGAC CCAGGATGAG CCCGAGCCAG
    ACTTTCGCTC AGGACAGAGA GAGAGAGAGA GTCCACCATC ATCATCACCA CCATCATCAT
    CGCTGTCAGA GACGCAGAGA CAAGAAGCAC AAGTCACTGG ATCGAGCCAT CAGCGAGGAG
    CAGCCGCCAC CTGCAGCTGA TCCAAACACA GAGTCGACGC CCAGGGAACG AGCCCGGGAG
    CGAGACCGTG GCCGCTCACA CGAGAGGAAG CACCACTCAT CCTCAGCAGC AGAAAGGAAG
    CAGCGATACT ACTCTTGTGA CCGCTACGGC AGCCATGAAC ATGGCCACAG CCTGTCCGCC
    GGGCCTAGCC GATCCACCTC TCCTGGAGAA CCACATGACA GGCTTAAACA GGGAATTAAC
    GCAGCTAAAG GAAGCTCAGT GGGTCATACC TCAGGCACCA GCACACCCTG CCGCGGGCGT
    AGACAGCTTC CCCAGACACC TCTTACCCCT CGGCCTGCTG TTGCCTATAA GACTGCCAAC
    TCTTCTCCGG TCCAGCTCAA CACTGCCCAA TCTACAGGGC CCTGCCCCGC TCGCCTTAGT
    CGTGGCCTGT CAGAGCACGA TACCTTACTC AGCGGTAGCA TCCACCGCCA ATCCCCTGTG
    CCTGTCACTC GTATTGGCTC TGACCCCAGC TTAGGTCCCC TCCAGCAAGA CTCGCACTTG
    TCTGAAGCGG AAGACTTTCA CGATGCCTTG AGCACGTACG GCACCGGTCG CTCCCCTAGA
    ACTACCGCCA TCATCGCCCA CACGTCATCT GGTGCGACCG CCACTTCTAC GCTGCTGTCC
    CGAAGCCAGA GCGGAGTCCC CAACGGGTAC CACTACTCCC TCGGAGTGAA CGCGGCTCCG
    GGATTGAGTG GGAGAGCGTC TGGAAGCTAC CAGGAGACTG AGAGAGATGA CTGGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146096
    Clone ID Related Accession (Same CDS sequence) XM_021473395.1
    Accession Version XM_021473395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCGAGCT TCATGGGATC GCAGCAAAGC GTGACCAGAG GAACTAAAGG AAAGGACATA
    AACACCATCA AGTCCCTGAG GGTTCTTCGA GTCTTGAGAC CCTTGAAGAC AATCAAAAGA
    TTGCCCAAAC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTGAAGAA TGTGCTCAAC
    ATCCTCATCG TCTACATCCT CTTCATGTTC ATCTTCGCGG TCATCGCCGT GCAGCTTTTC
    AAGGGGAAGT TTTTCCACTG CACCGATGAG TCCAAAGCCC TGGAGAAGGA CTGCAGGGGT
    CAGTTCTTGG AGTACGGCAG TGATGGAGTG GCCATGACGC AGCCGCGCGA GTGGAAGAAG
    TACGACTTCC ATTATGACAA CGTCCTGTGG GCCTTTCTGA CCCTCTTCAC TGTGTCTACA
    GGAGAGGGCT GGCCACTTGT GCTGAAACAC TCTGTTGATG CCACGTATGA AGACCAGGGT
    CCCAGTCCAG GATATCGCAT GGAAACCTCC ATCTTCTATG TGGTCTACTT CATCGTCTTT
    CCCTTCTTCT TTGTCAACAT CTTTGTGGCT TTGATCATCA TCACATTCCA GGAGCAGGGG
    GATAAAGCCA TGTCCGAGTG CAGCTTGGAA AAGAACGAGC GAGCTTGTAT TGACTTTGCC
    ATCAATGCTA AACCTCTGAC TCGCTACATG CCGCAAGACA AGAACTCTTA CCAGTACAAG
    ATGTGGAAGT TTGTGGTGTC GACTCCGTTT GAGTACTCCA TCCTGACCAT GATCGCCATC
    AATACAGTGG TGCTCATGAT GAAGTTCCAT GGTGCTCCTA AACCTTATGA GGACATGTTG
    AAGTGGCTGA ACATCATCTT CACTGCACTC TTCACACTGG AGTGTGTTCT CAAGGTCATC
    GCCTTCGGTC CTCTGAACTA CCTGAAAGAG GCTTGGAACA TTTTTGACTT TGTTACTGTT
    CTGGGAAGCA TCACAGACAT CCTGGTTACT GAGATCAAGA CGGATAAGAG GATTAATCTG
    AGTTTCCTGA GACTCTTCCG GGCGGCTCGT CTCATTAAAC TCCTTCGGCA AGGATACACC
    ATTCGGATCC TTCTCTGGAC CTTTGTTCAG TCCTTTAAGG CTCTTCCATA TGTGTGTCTC
    CTTATCGCAA TGCTTTTTTT CATTTACGCC ATCATCGGGA TGCAGGTGTT TGGTAACATC
    GCGTTGAACA ATGAAGATTC GGCCATCAAC ATTCACAATA ACTTCCGCAC CTTTTTTCAG
    GCCCTCATGT TGTTGTTCAG AAGTGCTACA GGGGAGGCTT GGCACGAGAT CATGTTGGCT
    TGTCTGAGTG AAAGATATTG CGATATAGAA TCTGGCTACA GTGGGAAAGA GTGCGGCAGC
    GATTTTGCAT ACTTCTACTT CGTCTCCTTC ATCTTTCTTT GCTCGTTTCT GATGCTGAAT
    CTGTTTGTGG CTGTCATCAT GGATAATTTT GAATATCTGA CACGAGATGC TTCTATTCTG
    GGGCCTCATC ACCTAGATGA GTTCATCCGC GTCTGGGCTG AGTACGACCC TGCCGCCTGT
    GGACGCATGT CCTACCTCGA TATGTACGAG ATGCTCCGCC ACATGTCCCC TCCGCTAGGT
    TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGAT TGGTGAGGAT GAACATGCCC
    ATAGCCGAGG ACAACACGGT TCACTTTACC TCCACTTTAA TGGCTCTCAT CCGGACTGCC
    CTGGAGATCA AACTGGCATC AGGAGTTCTG GCTCAGCGCA TTTGTGATGC TGAATTAAGG
    AAGGAAATCA ATAAAATTTG GCCCAGTTTG TCATCTAAAA CAGTAGACCT GCTGGTTACA
    CCTCATAAAC ATAACGAATT GACTGTCGGG AAGGTCTATG CAGCGCTCAT GATTTTTGAC
    TACTATAAGC AGAACAGAGC CAAAAGACTC CAACAGCAGC AAGCTGCACC AGGCACACAG
    AGTAAAGTCG GTGTTCTCTT CAGGCCGATG CTTCCTCTCA CACACCTGCA TGACCAGGAG
    CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT CATTTAGATA TCACCCCTCA GTCAGATGCC
    ACACCCACTT CTTTTCACTT GACCAATGGG GATGCAGTTC AAAGCCAGAG CAGTGCCATG
    AAAAAGTCTC CTTCGGGAGG CCCATCTCAG GAAGTCACAC AGGAAACGAA CAGAAGGCCT
    ATCCAACGTG GTCAATCGGA AGACGTGCCA AACACTTACA GATCTCAGGA ACTAGTAGAG
    ATGACTGACA TGGAGAATGA TTCGGATGTT GGCGGGTATC ATGTGCTGGA GGGTCATGGC
    AGAGCTGCCT CTATGCCCAG ACTGAATGCT GGCTTCCAGC GGAGCCACTT ACGCCACACC
    GCCGGAGCGT ATCTAGCACC AATTGCAGAT GTGAGTCCCA TGCGGCGATC CACATCCTCG
    CTGACTCCAC AAAGAGGTGC GCGAGAGGTC AACCTGAGCG AATACGACCT GGAGAGACCC
    AGGATGAGCC CGAGCCAGAC TTTCGCTCAG GACAGAGAGA GAGAGAGAGT CCACCATCAT
    CATCACCACC ATCATCATCG CTGTCAGAGA CGCAGAGACA AGAAGCACAA GTCACTGGAT
    CGAGCCATCA GCGAGGAGCA GCCGCCACCT GCAGCTGATC CAAACACAGA GTCGACGCCC
    AGGGAACGAG CCCGGGAGCG AGACCGTGGC CGCTCACACG AGAGGAAGCA CCACTCATCC
    TCAGCAGCAG AAAGGAAGCA GCGATACTAC TCTTGTGACC GCTACGGCAG CCATGAACAT
    GGCCACAGCC TGTCCGCCGG GCCTAGCCGA TCCACCTCTC CTGGAGAACC ACATGACAGG
    CTTAAACAGG GAATTAACGC AGCTAAAGGA AGCTCAGTGG GTCATACCTC AGGCACCAGC
    ACACCCTGCC GCGGGCGTAG ACAGCTTCCC CAGACACCTC TTACCCCTCG GCCTGCTGTT
    GCCTATAAGA CTGCCAACTC TTCTCCGGTC CAGCTCAACA CTGCCCAATC TACAGGGCCC
    TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA GAGCACGATA CCTTACTCAG CGGTAGCATC
    CACCGCCAAT CCCCTGTGCC TGTCACTCGT ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC
    CAGCAAGACT CGCACTTGTC TGAAGCGGAA GACTTTCACG ATGCCTTGAG CACGTACGGC
    ACCGGTCGCT CCCCTAGAAC TACCGCCATC ATCGCCCACA CGTCATCTGG TGCGACCGCC
    ACTTCTACGC TGCTGTCCCG AAGCCAGAGC GGAGTCCCCA ACGGGTACCA CTACTCCCTC
    GGAGTGAACG CGGCTCCGGG ATTGAGTGGG AGAGCGTCTG GAAGCTACCA GGAGACTGAG
    AGAGATGACT GGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329070.1
    CDS565..7602
    Translation

    Target ORF information:

    RefSeq Version XM_021473395.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCGAGCT TCATGGGATC GCAGCAAAGC GTGACCAGAG GAACTAAAGG AAAGGACATA
    AACACCATCA AGTCCCTGAG GGTTCTTCGA GTCTTGAGAC CCTTGAAGAC AATCAAAAGA
    TTGCCCAAAC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTGAAGAA TGTGCTCAAC
    ATCCTCATCG TCTACATCCT CTTCATGTTC ATCTTCGCGG TCATCGCCGT GCAGCTTTTC
    AAGGGGAAGT TTTTCCACTG CACCGATGAG TCCAAAGCCC TGGAGAAGGA CTGCAGGGGT
    CAGTTCTTGG AGTACGGCAG TGATGGAGTG GCCATGACGC AGCCGCGCGA GTGGAAGAAG
    TACGACTTCC ATTATGACAA CGTCCTGTGG GCCTTTCTGA CCCTCTTCAC TGTGTCTACA
    GGAGAGGGCT GGCCACTTGT GCTGAAACAC TCTGTTGATG CCACGTATGA AGACCAGGGT
    CCCAGTCCAG GATATCGCAT GGAAACCTCC ATCTTCTATG TGGTCTACTT CATCGTCTTT
    CCCTTCTTCT TTGTCAACAT CTTTGTGGCT TTGATCATCA TCACATTCCA GGAGCAGGGG
    GATAAAGCCA TGTCCGAGTG CAGCTTGGAA AAGAACGAGC GAGCTTGTAT TGACTTTGCC
    ATCAATGCTA AACCTCTGAC TCGCTACATG CCGCAAGACA AGAACTCTTA CCAGTACAAG
    ATGTGGAAGT TTGTGGTGTC GACTCCGTTT GAGTACTCCA TCCTGACCAT GATCGCCATC
    AATACAGTGG TGCTCATGAT GAAGTTCCAT GGTGCTCCTA AACCTTATGA GGACATGTTG
    AAGTGGCTGA ACATCATCTT CACTGCACTC TTCACACTGG AGTGTGTTCT CAAGGTCATC
    GCCTTCGGTC CTCTGAACTA CCTGAAAGAG GCTTGGAACA TTTTTGACTT TGTTACTGTT
    CTGGGAAGCA TCACAGACAT CCTGGTTACT GAGATCAAGA CGGATAAGAG GATTAATCTG
    AGTTTCCTGA GACTCTTCCG GGCGGCTCGT CTCATTAAAC TCCTTCGGCA AGGATACACC
    ATTCGGATCC TTCTCTGGAC CTTTGTTCAG TCCTTTAAGG CTCTTCCATA TGTGTGTCTC
    CTTATCGCAA TGCTTTTTTT CATTTACGCC ATCATCGGGA TGCAGGTGTT TGGTAACATC
    GCGTTGAACA ATGAAGATTC GGCCATCAAC ATTCACAATA ACTTCCGCAC CTTTTTTCAG
    GCCCTCATGT TGTTGTTCAG AAGTGCTACA GGGGAGGCTT GGCACGAGAT CATGTTGGCT
    TGTCTGAGTG AAAGATATTG CGATATAGAA TCTGGCTACA GTGGGAAAGA GTGCGGCAGC
    GATTTTGCAT ACTTCTACTT CGTCTCCTTC ATCTTTCTTT GCTCGTTTCT GATGCTGAAT
    CTGTTTGTGG CTGTCATCAT GGATAATTTT GAATATCTGA CACGAGATGC TTCTATTCTG
    GGGCCTCATC ACCTAGATGA GTTCATCCGC GTCTGGGCTG AGTACGACCC TGCCGCCTGT
    GGACGCATGT CCTACCTCGA TATGTACGAG ATGCTCCGCC ACATGTCCCC TCCGCTAGGT
    TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGAT TGGTGAGGAT GAACATGCCC
    ATAGCCGAGG ACAACACGGT TCACTTTACC TCCACTTTAA TGGCTCTCAT CCGGACTGCC
    CTGGAGATCA AACTGGCATC AGGAGTTCTG GCTCAGCGCA TTTGTGATGC TGAATTAAGG
    AAGGAAATCA ATAAAATTTG GCCCAGTTTG TCATCTAAAA CAGTAGACCT GCTGGTTACA
    CCTCATAAAC ATAACGAATT GACTGTCGGG AAGGTCTATG CAGCGCTCAT GATTTTTGAC
    TACTATAAGC AGAACAGAGC CAAAAGACTC CAACAGCAGC AAGCTGCACC AGGCACACAG
    AGTAAAGTCG GTGTTCTCTT CAGGCCGATG CTTCCTCTCA CACACCTGCA TGACCAGGAG
    CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT CATTTAGATA TCACCCCTCA GTCAGATGCC
    ACACCCACTT CTTTTCACTT GACCAATGGG GATGCAGTTC AAAGCCAGAG CAGTGCCATG
    AAAAAGTCTC CTTCGGGAGG CCCATCTCAG GAAGTCACAC AGGAAACGAA CAGAAGGCCT
    ATCCAACGTG GTCAATCGGA AGACGTGCCA AACACTTACA GATCTCAGGA ACTAGTAGAG
    ATGACTGACA TGGAGAATGA TTCGGATGTT GGCGGGTATC ATGTGCTGGA GGGTCATGGC
    AGAGCTGCCT CTATGCCCAG ACTGAATGCT GGCTTCCAGC GGAGCCACTT ACGCCACACC
    GCCGGAGCGT ATCTAGCACC AATTGCAGAT GTGAGTCCCA TGCGGCGATC CACATCCTCG
    CTGACTCCAC AAAGAGGTGC GCGAGAGGTC AACCTGAGCG AATACGACCT GGAGAGACCC
    AGGATGAGCC CGAGCCAGAC TTTCGCTCAG GACAGAGAGA GAGAGAGAGT CCACCATCAT
    CATCACCACC ATCATCATCG CTGTCAGAGA CGCAGAGACA AGAAGCACAA GTCACTGGAT
    CGAGCCATCA GCGAGGAGCA GCCGCCACCT GCAGCTGATC CAAACACAGA GTCGACGCCC
    AGGGAACGAG CCCGGGAGCG AGACCGTGGC CGCTCACACG AGAGGAAGCA CCACTCATCC
    TCAGCAGCAG AAAGGAAGCA GCGATACTAC TCTTGTGACC GCTACGGCAG CCATGAACAT
    GGCCACAGCC TGTCCGCCGG GCCTAGCCGA TCCACCTCTC CTGGAGAACC ACATGACAGG
    CTTAAACAGG GAATTAACGC AGCTAAAGGA AGCTCAGTGG GTCATACCTC AGGCACCAGC
    ACACCCTGCC GCGGGCGTAG ACAGCTTCCC CAGACACCTC TTACCCCTCG GCCTGCTGTT
    GCCTATAAGA CTGCCAACTC TTCTCCGGTC CAGCTCAACA CTGCCCAATC TACAGGGCCC
    TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA GAGCACGATA CCTTACTCAG CGGTAGCATC
    CACCGCCAAT CCCCTGTGCC TGTCACTCGT ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC
    CAGCAAGACT CGCACTTGTC TGAAGCGGAA GACTTTCACG ATGCCTTGAG CACGTACGGC
    ACCGGTCGCT CCCCTAGAAC TACCGCCATC ATCGCCCACA CGTCATCTGG TGCGACCGCC
    ACTTCTACGC TGCTGTCCCG AAGCCAGAGC GGAGTCCCCA ACGGGTACCA CTACTCCCTC
    GGAGTGAACG CGGCTCCGGG ATTGAGTGGG AGAGCGTCTG GAAGCTACCA GGAGACTGAG
    AGAGATGACT GGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146097
    Clone ID Related Accession (Same CDS sequence) XM_021473396.1
    Accession Version XM_021473396.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC
    ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC
    TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC
    ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT
    CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG
    GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT
    GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC
    TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA
    TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC
    TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG
    CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC
    AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC
    ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT
    CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA
    TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG
    GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA
    CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT
    CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT
    TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT
    GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA
    GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC
    ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC
    GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC
    TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG
    AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC
    CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC
    AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC
    AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA
    GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC
    TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT
    TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC
    ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC
    TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG
    ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG
    CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG
    CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT
    GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC
    TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC
    GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA
    GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA
    GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329071.1
    CDS565..7569
    Translation

    Target ORF information:

    RefSeq Version XM_021473396.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473396.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC
    ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC
    TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC
    ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT
    CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG
    GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT
    GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC
    TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA
    TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC
    TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG
    CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC
    AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC
    ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT
    CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA
    TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG
    GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA
    CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT
    CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT
    TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT
    GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA
    GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC
    ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC
    GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC
    TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG
    AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC
    CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC
    AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC
    AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA
    GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC
    TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT
    TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC
    ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC
    TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG
    ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG
    CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG
    CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT
    GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC
    TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC
    GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA
    GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA
    GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146098
    Clone ID Related Accession (Same CDS sequence) XM_021473397.1
    Accession Version XM_021473397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT
    AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT
    AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC
    GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC
    AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG
    GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC
    TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT
    CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT
    CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG
    GCTGAGTACG ACCCTGCCGC CTGTGGACGC ATGTCCTACC TCGATATGTA CGAGATGCTC
    CGCCACATGT CCCCTCCGCT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT
    TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG
    CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT
    AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC
    TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG
    CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT
    CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA
    GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA
    GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC
    ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT
    TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG
    TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC
    CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT
    CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG
    AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA
    GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA
    GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT
    GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA
    CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT
    GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC
    TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA
    GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA
    CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC
    AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC
    GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC
    TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT
    CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC
    CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC
    CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG
    TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329072.1
    CDS566..7567
    Translation

    Target ORF information:

    RefSeq Version XM_021473397.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT
    AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT
    AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC
    GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC
    AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG
    GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC
    TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT
    CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT
    CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG
    GCTGAGTACG ACCCTGCCGC CTGTGGACGC ATGTCCTACC TCGATATGTA CGAGATGCTC
    CGCCACATGT CCCCTCCGCT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT
    TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG
    CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT
    AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC
    TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG
    CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT
    CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA
    GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA
    GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC
    ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT
    TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG
    TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC
    CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT
    CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG
    AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA
    GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA
    GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT
    GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA
    CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT
    GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC
    TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA
    GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA
    CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC
    AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC
    GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC
    TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT
    CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC
    CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC
    CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG
    TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146099
    Clone ID Related Accession (Same CDS sequence) XM_021473398.1
    Accession Version XM_021473398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT
    AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT
    AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC
    GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC
    AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG
    GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC
    TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT
    CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT
    CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG
    GCTGAGTACG ACCCTGCCGC CTGTGGAAGG ATTCCCTATA TGGATATGTA CAATTTGTTG
    CGTGTAATCT CACCTCCCCT TGGCTTAGGA AAGAACTGCC CTAATAGGGT GGCATACAAG
    CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT
    TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG
    CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT
    AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC
    TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG
    CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT
    CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA
    GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA
    GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC
    ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT
    TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG
    TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC
    CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT
    CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG
    AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA
    GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA
    GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT
    GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA
    CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT
    GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC
    TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA
    GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA
    CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC
    AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC
    GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC
    TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT
    CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC
    CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC
    CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG
    TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329073.1
    CDS566..7567
    Translation

    Target ORF information:

    RefSeq Version XM_021473398.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT
    AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT
    AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC
    GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC
    AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG
    GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC
    TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT
    CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT
    CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG
    GCTGAGTACG ACCCTGCCGC CTGTGGAAGG ATTCCCTATA TGGATATGTA CAATTTGTTG
    CGTGTAATCT CACCTCCCCT TGGCTTAGGA AAGAACTGCC CTAATAGGGT GGCATACAAG
    CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT
    TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG
    CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT
    AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC
    TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG
    CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT
    CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA
    GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA
    GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC
    ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT
    TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG
    TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC
    CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT
    CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG
    AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA
    GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA
    GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT
    GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA
    CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT
    GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC
    TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA
    GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA
    CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC
    AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC
    GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC
    TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT
    CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC
    CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC
    CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG
    TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151030
    Clone ID Related Accession (Same CDS sequence) XM_021469036.1
    Accession Version XM_021469036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCGAGCT TCATGGGATC GCAGCAAAGC GTGACCAGAG GAACTAAAGG AAAGGACATA
    AACACCATCA AGTCCCTGAG GGTTCTTCGA GTCTTGAGAC CCTTGAAGAC AATCAAAAGA
    TTGCCCAAAC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTGAAGAA TGTGCTCAAC
    ATCCTCATCG TCTACATCCT CTTCATGTTC ATCTTCGCGG TCATCGCCGT GCAGCTTTTC
    AAGGGGAAGT TTTTCCACTG CACCGATGAG TCCAAAGCCC TGGAGAAGGA CTGCAGGGGT
    CAGTTCTTGG AGTACGGCAG TGATGGAGTG GCCATGACGC AGCCGCGCGA GTGGAAGAAG
    TACGACTTCC ATTATGACAA CGTCCTGTGG GCCTTTCTGA CCCTCTTCAC TGTGTCTACA
    GGAGAGGGCT GGCCACTTGT GCTGAAACAC TCTGTTGATG CCACGTATGA AGACCAGGGT
    CCCAGTCCAG GATATCGCAT GGAAACCTCC ATCTTCTATG TGGTCTACTT CATCGTCTTT
    CCCTTCTTCT TTGTCAACAT CTTTGTGGCT TTGATCATCA TCACATTCCA GGAGCAGGGG
    GATAAAGCCA TGTCCGAGTG CAGCTTGGAA AAGAACGAGC GAGCTTGTAT TGACTTTGCC
    ATCAATGCTA AACCTCTGAC TCGCTACATG CCGCAAGACA AGAACTCTTA CCAGTACAAG
    ATGTGGAAGT TTGTGGTGTC GACTCCGTTT GAGTACTCCA TCCTGACCAT GATCGCCATC
    AATACAGTGG TGCTCATGAT GAAGTTCCAT GGTGCTCCTA AACCTTATGA GGACATGTTG
    AAGTGGCTGA ACATCATCTT CACTGCACTC TTCACACTGG AGTGTGTTCT CAAGGTCATC
    GCCTTCGGTC CTCTGAACTA CCTGAAAGAG GCTTGGAACA TTTTTGACTT TGTTACTGTT
    CTGGGAAGCA TCACAGACAT CCTGGTTACT GAGATCAAGA CGGATAAGAG GATTAATCTG
    AGTTTCCTGA GACTCTTCCG GGCGGCTCGT CTCATTAAAC TCCTTCGGCA AGGATACACC
    ATTCGGATCC TTCTCTGGAC CTTTGTTCAG TCCTTTAAGG CTCTTCCATA TGTGTGTCTC
    CTTATCGCAA TGCTTTTTTT CATTTACGCC ATCATCGGGA TGCAGGTGTT TGGTAACATC
    GCGTTGAACA ATGAAGATTC GGCCATCAAC ATTCACAATA ACTTCCGCAC CTTTTTTCAG
    GCCCTCATGT TGTTGTTCAG AAGTGCTACA GGGGAGGCTT GGCACGAGAT CATGTTGGCT
    TGTCTGAGTG AAAGATATTG CGATATAGAA TCTGGCTACA GTGGGAAAGA GTGCGGCAGC
    GATTTTGCAT ACTTCTACTT CGTCTCCTTC ATCTTTCTTT GCTCGTTTCT GATGCTGAAT
    CTGTTTGTGG CTGTCATCAT GGATAATTTT GAATATCTGA CACGAGATGC TTCTATTCTG
    GGGCCTCATC ACCTAGATGA GTTCATCCGC GTCTGGGCTG AGTACGACCC TGCCGCCTGT
    GGACGCATGT CCTACCTCGA TATGTACGAG ATGCTCCGCC ACATGTCCCC TCCGCTAGGT
    TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGAT TGGTGAGGAT GAACATGCCC
    ATAGCCGAGG ACAACACGGT TCACTTTACC TCCACTTTAA TGGCTCTCAT CCGGACTGCC
    CTGGAGATCA AACTGGCATC AGGAGTTCTG GCTCAGCGCA TTTGTGATGC TGAATTAAGG
    AAGGAAATCA ATAAAATTTG GCCCAGTTTG TCATCTAAAA CAGTAGACCT GCTGGTTACA
    CCTCATAAAC ATAACGAATT GACTGTCGGG AAGGTCTATG CAGCGCTCAT GATTTTTGAC
    TACTATAAGC AGAACAGAGC CAAAAGACTC CAACAGCAGC AAGCTGCACC AGGCACACAG
    AGTAAAGTCG GTGTTCTCTT CAGGCCGATG CTTCCTCTCA CACACCTGCA TGACCAGGAG
    CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT CATTTAGATA TCACCCCTCA GTCAGATGCC
    ACACCCACTT CTTTTCACTT GACCAATGGG GATGCAGTTC AAAGCCAGAG CAGTGCCATG
    AAAAAGTCTC CTTCGGGAGG CCCATCTCAG GAAGTCACAC AGGAAACGAA CAGAAGGCCT
    ATCCAACGTG GTCAATCGGA AGACGTGCCA AACACTTACA GATCTCAGGA ACTAGTAGAG
    ATGACTGACA TGGAGAATGA TTCGGATGTT GGCGGGTATC ATGTGCTGGA GGGTCATGGC
    AGAGCTGCCT CTATGCCCAG ACTGAATGCT GGCTTCCAGC GGAGCCACTT ACGCCACACC
    GCCGGAGCGT ATCTAGCACC AATTGCAGAT GTGAGTCCCA TGCGGCGATC CACATCCTCG
    CTGACTCCAC AAAGAGGTGC GCGAGAGGTC AACCTGAGCG AATACGACCT GGAGAGACCC
    AGGATGAGCC CGAGCCAGAC TTTCGCTCAG GACAGAGAGA GAGAGAGAGT CCACCATCAT
    CATCACCACC ATCATCATCG CTGTCAGAGA CGCAGAGACA AGAAGCACAA GTCACTGGAT
    CGAGCCATCA GCGAGGAGCA GCCGCCACCT GCAGCTGATC CAAACACAGA GTCGACGCCC
    AGGGAACGAG CCCGGGAGCG AGACCGTGGC CGCTCACACG AGAGGAAGCA CCACTCATCC
    TCAGCAGCAG AAAGGAAGCA GCGATACTAC TCTTGTGACC GCTACGGCAG CCATGAACAT
    GGCCACAGCC TGTCCGCCGG GCCTAGCCGA TCCACCTCTC CTGGAGAACC ACATGACAGG
    CTTAAACAGG GAATTAACGC AGCTAAAGGA AGCTCAGTGG GTCATACCTC AGGCACCAGC
    ACACCCTGCC GCGGGCGTAG ACAGCTTCCC CAGACACCTC TTACCCCTCG GCCTGCTGTT
    GCCTATAAGA CTGCCAACTC TTCTCCGGTC CAGCTCAACA CTGCCCAATC TACAGGGCCC
    TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA GAGCACGATA CCTTACTCAG CGGTAGCATC
    CACCGCCAAT CCCCTGTGCC TGTCACTCGT ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC
    CAGCAAGACT CGCACTTGTC TGAAGCGGAA GACTTTCACG ATGCCTTGAG CACGTACGGC
    ACCGGTCGCT CCCCTAGAAC TACCGCCATC ATCGCCCACA CGTCATCTGG TGCGACCGCC
    ACTTCTACGC TGCTGTCCCG AAGCCAGAGC GGAGTCCCCA ACGGGTACCA CTACTCCCTC
    GGAGTGAACG CGGCTCCGGG ATTGAGTGGG AGAGCGTCTG GAAGCTACCA GGAGACTGAG
    AGAGATGACT GGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324711.1
    CDS556..7593
    Translation

    Target ORF information:

    RefSeq Version XM_021469036.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCGAGCT TCATGGGATC GCAGCAAAGC GTGACCAGAG GAACTAAAGG AAAGGACATA
    AACACCATCA AGTCCCTGAG GGTTCTTCGA GTCTTGAGAC CCTTGAAGAC AATCAAAAGA
    TTGCCCAAAC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTGAAGAA TGTGCTCAAC
    ATCCTCATCG TCTACATCCT CTTCATGTTC ATCTTCGCGG TCATCGCCGT GCAGCTTTTC
    AAGGGGAAGT TTTTCCACTG CACCGATGAG TCCAAAGCCC TGGAGAAGGA CTGCAGGGGT
    CAGTTCTTGG AGTACGGCAG TGATGGAGTG GCCATGACGC AGCCGCGCGA GTGGAAGAAG
    TACGACTTCC ATTATGACAA CGTCCTGTGG GCCTTTCTGA CCCTCTTCAC TGTGTCTACA
    GGAGAGGGCT GGCCACTTGT GCTGAAACAC TCTGTTGATG CCACGTATGA AGACCAGGGT
    CCCAGTCCAG GATATCGCAT GGAAACCTCC ATCTTCTATG TGGTCTACTT CATCGTCTTT
    CCCTTCTTCT TTGTCAACAT CTTTGTGGCT TTGATCATCA TCACATTCCA GGAGCAGGGG
    GATAAAGCCA TGTCCGAGTG CAGCTTGGAA AAGAACGAGC GAGCTTGTAT TGACTTTGCC
    ATCAATGCTA AACCTCTGAC TCGCTACATG CCGCAAGACA AGAACTCTTA CCAGTACAAG
    ATGTGGAAGT TTGTGGTGTC GACTCCGTTT GAGTACTCCA TCCTGACCAT GATCGCCATC
    AATACAGTGG TGCTCATGAT GAAGTTCCAT GGTGCTCCTA AACCTTATGA GGACATGTTG
    AAGTGGCTGA ACATCATCTT CACTGCACTC TTCACACTGG AGTGTGTTCT CAAGGTCATC
    GCCTTCGGTC CTCTGAACTA CCTGAAAGAG GCTTGGAACA TTTTTGACTT TGTTACTGTT
    CTGGGAAGCA TCACAGACAT CCTGGTTACT GAGATCAAGA CGGATAAGAG GATTAATCTG
    AGTTTCCTGA GACTCTTCCG GGCGGCTCGT CTCATTAAAC TCCTTCGGCA AGGATACACC
    ATTCGGATCC TTCTCTGGAC CTTTGTTCAG TCCTTTAAGG CTCTTCCATA TGTGTGTCTC
    CTTATCGCAA TGCTTTTTTT CATTTACGCC ATCATCGGGA TGCAGGTGTT TGGTAACATC
    GCGTTGAACA ATGAAGATTC GGCCATCAAC ATTCACAATA ACTTCCGCAC CTTTTTTCAG
    GCCCTCATGT TGTTGTTCAG AAGTGCTACA GGGGAGGCTT GGCACGAGAT CATGTTGGCT
    TGTCTGAGTG AAAGATATTG CGATATAGAA TCTGGCTACA GTGGGAAAGA GTGCGGCAGC
    GATTTTGCAT ACTTCTACTT CGTCTCCTTC ATCTTTCTTT GCTCGTTTCT GATGCTGAAT
    CTGTTTGTGG CTGTCATCAT GGATAATTTT GAATATCTGA CACGAGATGC TTCTATTCTG
    GGGCCTCATC ACCTAGATGA GTTCATCCGC GTCTGGGCTG AGTACGACCC TGCCGCCTGT
    GGACGCATGT CCTACCTCGA TATGTACGAG ATGCTCCGCC ACATGTCCCC TCCGCTAGGT
    TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGAT TGGTGAGGAT GAACATGCCC
    ATAGCCGAGG ACAACACGGT TCACTTTACC TCCACTTTAA TGGCTCTCAT CCGGACTGCC
    CTGGAGATCA AACTGGCATC AGGAGTTCTG GCTCAGCGCA TTTGTGATGC TGAATTAAGG
    AAGGAAATCA ATAAAATTTG GCCCAGTTTG TCATCTAAAA CAGTAGACCT GCTGGTTACA
    CCTCATAAAC ATAACGAATT GACTGTCGGG AAGGTCTATG CAGCGCTCAT GATTTTTGAC
    TACTATAAGC AGAACAGAGC CAAAAGACTC CAACAGCAGC AAGCTGCACC AGGCACACAG
    AGTAAAGTCG GTGTTCTCTT CAGGCCGATG CTTCCTCTCA CACACCTGCA TGACCAGGAG
    CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT CATTTAGATA TCACCCCTCA GTCAGATGCC
    ACACCCACTT CTTTTCACTT GACCAATGGG GATGCAGTTC AAAGCCAGAG CAGTGCCATG
    AAAAAGTCTC CTTCGGGAGG CCCATCTCAG GAAGTCACAC AGGAAACGAA CAGAAGGCCT
    ATCCAACGTG GTCAATCGGA AGACGTGCCA AACACTTACA GATCTCAGGA ACTAGTAGAG
    ATGACTGACA TGGAGAATGA TTCGGATGTT GGCGGGTATC ATGTGCTGGA GGGTCATGGC
    AGAGCTGCCT CTATGCCCAG ACTGAATGCT GGCTTCCAGC GGAGCCACTT ACGCCACACC
    GCCGGAGCGT ATCTAGCACC AATTGCAGAT GTGAGTCCCA TGCGGCGATC CACATCCTCG
    CTGACTCCAC AAAGAGGTGC GCGAGAGGTC AACCTGAGCG AATACGACCT GGAGAGACCC
    AGGATGAGCC CGAGCCAGAC TTTCGCTCAG GACAGAGAGA GAGAGAGAGT CCACCATCAT
    CATCACCACC ATCATCATCG CTGTCAGAGA CGCAGAGACA AGAAGCACAA GTCACTGGAT
    CGAGCCATCA GCGAGGAGCA GCCGCCACCT GCAGCTGATC CAAACACAGA GTCGACGCCC
    AGGGAACGAG CCCGGGAGCG AGACCGTGGC CGCTCACACG AGAGGAAGCA CCACTCATCC
    TCAGCAGCAG AAAGGAAGCA GCGATACTAC TCTTGTGACC GCTACGGCAG CCATGAACAT
    GGCCACAGCC TGTCCGCCGG GCCTAGCCGA TCCACCTCTC CTGGAGAACC ACATGACAGG
    CTTAAACAGG GAATTAACGC AGCTAAAGGA AGCTCAGTGG GTCATACCTC AGGCACCAGC
    ACACCCTGCC GCGGGCGTAG ACAGCTTCCC CAGACACCTC TTACCCCTCG GCCTGCTGTT
    GCCTATAAGA CTGCCAACTC TTCTCCGGTC CAGCTCAACA CTGCCCAATC TACAGGGCCC
    TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA GAGCACGATA CCTTACTCAG CGGTAGCATC
    CACCGCCAAT CCCCTGTGCC TGTCACTCGT ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC
    CAGCAAGACT CGCACTTGTC TGAAGCGGAA GACTTTCACG ATGCCTTGAG CACGTACGGC
    ACCGGTCGCT CCCCTAGAAC TACCGCCATC ATCGCCCACA CGTCATCTGG TGCGACCGCC
    ACTTCTACGC TGCTGTCCCG AAGCCAGAGC GGAGTCCCCA ACGGGTACCA CTACTCCCTC
    GGAGTGAACG CGGCTCCGGG ATTGAGTGGG AGAGCGTCTG GAAGCTACCA GGAGACTGAG
    AGAGATGACT GGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151031
    Clone ID Related Accession (Same CDS sequence) XM_017352992.2
    Accession Version XM_017352992.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC
    ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC
    TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC
    ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT
    CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG
    GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT
    GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC
    TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA
    TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC
    TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG
    CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC
    AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC
    ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT
    CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA
    TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG
    GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA
    CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT
    CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT
    TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT
    GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA
    GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC
    ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC
    GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC
    TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG
    AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC
    CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC
    AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC
    AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA
    GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC
    TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT
    TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC
    ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC
    TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG
    ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG
    CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG
    CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT
    GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC
    TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC
    GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA
    GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA
    GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208481.2
    CDS556..7560
    Translation

    Target ORF information:

    RefSeq Version XM_017352992.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352992.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC
    ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC
    TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC
    ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT
    CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG
    GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT
    GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC
    TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA
    TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC
    TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG
    CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC
    AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC
    ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT
    CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA
    TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG
    GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA
    CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT
    CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT
    TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT
    GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA
    GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC
    ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC
    GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC
    TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG
    AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC
    CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC
    AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC
    AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA
    GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC
    TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT
    TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC
    ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC
    TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG
    ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG
    CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG
    CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT
    GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC
    TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC
    GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA
    GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA
    GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151032
    Clone ID Related Accession (Same CDS sequence) XM_017352993.2
    Accession Version XM_017352993.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT
    AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT
    AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC
    GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC
    AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG
    GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC
    TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT
    CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT
    CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG
    GCTGAGTACG ACCCTGCCGC CTGTGGACGC ATGTCCTACC TCGATATGTA CGAGATGCTC
    CGCCACATGT CCCCTCCGCT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT
    TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG
    CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT
    AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC
    TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG
    CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT
    CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA
    GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA
    GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC
    ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT
    TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG
    TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC
    CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT
    CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG
    AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA
    GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA
    GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT
    GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA
    CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT
    GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC
    TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA
    GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA
    CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC
    AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC
    GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC
    TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT
    CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC
    CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC
    CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG
    TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208482.2
    CDS556..7557
    Translation

    Target ORF information:

    RefSeq Version XM_017352993.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352993.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT
    AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT
    AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC
    GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC
    AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG
    GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC
    TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT
    CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT
    CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG
    GCTGAGTACG ACCCTGCCGC CTGTGGACGC ATGTCCTACC TCGATATGTA CGAGATGCTC
    CGCCACATGT CCCCTCCGCT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT
    TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG
    CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT
    AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC
    TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG
    CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT
    CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA
    GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA
    GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC
    ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT
    TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG
    TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC
    CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT
    CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG
    AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA
    GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA
    GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT
    GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA
    CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT
    GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC
    TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA
    GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA
    CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC
    AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC
    GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC
    TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT
    CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC
    CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC
    CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG
    TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151033
    Clone ID Related Accession (Same CDS sequence) XM_017352994.2
    Accession Version XM_017352994.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT
    AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT
    AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC
    GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC
    AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG
    GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC
    TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT
    CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT
    CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG
    GCTGAGTACG ACCCTGCCGC CTGTGGAAGG ATTCCCTATA TGGATATGTA CAATTTGTTG
    CGTGTAATCT CACCTCCCCT TGGCTTAGGA AAGAACTGCC CTAATAGGGT GGCATACAAG
    CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT
    TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG
    CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT
    AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC
    TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG
    CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT
    CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA
    GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA
    GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC
    ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT
    TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG
    TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC
    CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT
    CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG
    AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA
    GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA
    GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT
    GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA
    CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT
    GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC
    TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA
    GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA
    CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC
    AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC
    GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC
    TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT
    CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC
    CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC
    CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG
    TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208483.2
    CDS556..7557
    Translation

    Target ORF information:

    RefSeq Version XM_017352994.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352994.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
    GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG
    TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC
    TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT
    GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC
    AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT
    GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT
    AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC
    ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC
    TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA
    AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA
    TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC
    AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT
    GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA
    GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC
    CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA
    AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT
    AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG
    AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC
    TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG
    GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA
    AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT
    TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG
    AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC
    ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG
    AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG
    TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC
    GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT
    GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC
    AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT
    TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC
    CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG
    TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG
    ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG
    TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC
    TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT
    GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT
    CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC
    GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT
    CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT
    AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG
    CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC
    CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA
    GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT
    CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA
    GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC
    AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT
    CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC
    AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA
    CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT
    GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT
    CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA
    AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA
    ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG
    CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA
    ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC
    ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT
    CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA
    TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC
    AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC
    TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC
    TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG
    GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC
    TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC
    AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC
    ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC
    TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT
    GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC
    TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG
    ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG
    AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG
    CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG
    TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT
    GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC
    ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT
    TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG
    ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT
    AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT
    AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC
    GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC
    AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG
    GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC
    TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT
    CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT
    CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG
    GCTGAGTACG ACCCTGCCGC CTGTGGAAGG ATTCCCTATA TGGATATGTA CAATTTGTTG
    CGTGTAATCT CACCTCCCCT TGGCTTAGGA AAGAACTGCC CTAATAGGGT GGCATACAAG
    CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT
    TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG
    CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT
    AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC
    TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG
    CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT
    CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA
    GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA
    GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC
    ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT
    TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG
    TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC
    CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT
    CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG
    AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA
    GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA
    GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT
    GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA
    CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT
    GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC
    TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA
    GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA
    CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC
    AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC
    GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC
    TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT
    CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC
    CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC
    CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG
    TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56940
    Clone ID Related Accession (Same CDS sequence) XM_017352991.1
    Accession Version XM_017352991.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
    GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC
    TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG
    TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA
    ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG
    ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC
    ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT
    CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC
    TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA
    GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC
    GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC
    ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG
    GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC
    GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC
    GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT
    CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC
    GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC
    CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC
    TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG
    GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG
    GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG
    ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG
    GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT
    GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG
    AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG
    CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG
    GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC
    ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC
    ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT
    ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT
    GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT
    CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC
    TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC
    AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG
    CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA
    TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG
    ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT
    GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT
    AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA
    AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC
    CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA
    CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT
    GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA
    AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG
    AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC
    CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG
    GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC
    ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT
    GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT
    GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG
    ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG
    CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG
    TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT
    TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT
    TTCGCCTGCT CGAGCTTCAT GGGAGGAACT AAAGGAAAGG ACATAAACAC CATCAAGTCC
    CTGAGGGTTC TTCGAGTCTT GAGACCCTTG AAGACAATCA AAAGATTGCC CAAACTCAAG
    GCCGTGTTTG ACTGCGTGGT GAACTCTCTG AAGAATGTGC TCAACATCCT CATCGTCTAC
    ATCCTCTTCA TGTTCATCTT CGCGGTCATC GCCGTGCAGC TTTTCAAGGG GAAGTTTTTC
    CACTGCACCG ATGAGTCCAA AGCCCTGGAG AAGGACTGCA GGGGTCAGTT CTTGGAGTAC
    GGCAGTGATG GAGTGGCCAT GACGCAGCCG CGCGAGTGGA AGAAGTACGA CTTCCATTAT
    GACAACGTCC TGTGGGCCTT TCTGACCCTC TTCACTGTGT CTACAGGAGA GGGCTGGCCA
    CTTGTGCTGA AACACTCTGT TGATGCCACG TATGAAGACC AGGGTCCCAG TCCAGGATAT
    CGCATGGAAA CCTCCATCTT CTATGTGGTC TACTTCATCG TCTTTCCCTT CTTCTTTGTC
    AACATCTTTG TGGCTTTGAT CATCATCACA TTCCAGGAGC AGGGGGATAA AGCCATGTCC
    GAGTGCAGCT TGGAAAAGAA CGAGCGAGCT TGTATTGACT TTGCCATCAA TGCTAAACCT
    CTGACTCGCT ACATGCCGCA AGACAAGAAC TCTTACCAGT ACAAGATGTG GAAGTTTGTG
    GTGTCGACTC CGTTTGAGTA CTCCATCCTG ACCATGATCG CCATCAATAC AGTGGTGCTC
    ATGATGAAGT TCCATGGTGC TCCTAAACCT TATGAGGACA TGTTGAAGTG GCTGAACATC
    ATCTTCACTG CACTCTTCAC ACTGGAGTGT GTTCTCAAGG TCATCGCCTT CGGTCCTCTG
    AACTACCTGA AAGAGGCTTG GAACATTTTT GACTTTGTTA CTGTTCTGGG AAGCATCACA
    GACATCCTGG TTACTGAGAT CAAGACGGAT AAGAGGATTA ATCTGAGTTT CCTGAGACTC
    TTCCGGGCGG CTCGTCTCAT TAAACTCCTT CGGCAAGGAT ACACCATTCG GATCCTTCTC
    TGGACCTTTG TTCAGTCCTT TAAGGCTCTT CCATATGTGT GTCTCCTTAT CGCAATGCTT
    TTTTTCATTT ACGCCATCAT CGGGATGCAG GTGTTTGGTA ACATCGCGTT GAACAATGAA
    GATTCGGCCA TCAACATTCA CAATAACTTC CGCACCTTTT TTCAGGCCCT CATGTTGTTG
    TTCAGAAGTG CTACAGGGGA GGCTTGGCAC GAGATCATGT TGGCTTGTCT GAGTGAAAGA
    TATTGCGATA TAGAATCTGG CTACAGTGGG AAAGAGTGCG GCAGCGATTT TGCATACTTC
    TACTTCGTCT CCTTCATCTT TCTTTGCTCG TTTCTGATGC TGAATCTGTT TGTGGCTGTC
    ATCATGGATA ATTTTGAATA TCTGACACGA GATGCTTCTA TTCTGGGGCC TCATCACCTA
    GATGAGTTCA TCCGCGTCTG GGCTGAGTAC GACCCTGCCG CCTGTGGAAG GATTCCCTAT
    ATGGATATGT ACAATTTGTT GCGTGTAATC TCACCTCCCC TTGGCTTAGG AAAGAACTGC
    CCTAATAGGG TGGCATACAA GCGATTGGTG AGGATGAACA TGCCCATAGC CGAGGACAAC
    ACGGTTCACT TTACCTCCAC TTTAATGGCT CTCATCCGGA CTGCCCTGGA GATCAAACTG
    GCATCAGGAG TTCTGGCTCA GCGCATTTGT GATGCTGAAT TAAGGAAGGA AATCAATAAA
    ATTTGGCCCA GTTTGTCATC TAAAACAGTA GACCTGCTGG TTACACCTCA TAAACATAAC
    GAATTGACTG TCGGGAAGGT CTATGCAGCG CTCATGATTT TTGACTACTA TAAGCAGAAC
    AGAGCCAAAA GACTCCAACA GCAGCAAGCT GCACCAGGCA CACAGAGTAA AGTCGGTGTT
    CTCTTCAGGC CGATGCTTCC TCTCACACAC CTGCATGACC AGGAGCCTTT AGTGCCAAAA
    GGCCCGCCTC CTTCTCATTT AGATATCACC CCTCAGTCAG ATGCCACACC CACTTCTTTT
    CACTTGACCA ATGGGGATGC AGTTCAAAGC CAGAGCAGTG CCATGAAAAA GTCTCCTTCG
    GGAGGCCCAT CTCAGGAAGT CACACAGGAA ACGAACAGAA GGCCTATCCA ACGTGGTCAA
    TCGGAAGACG TGCCAAACAC TTACAGATCT CAGGAACTAG TAGAGATGAC TGACATGGAG
    AATGATTCGG ATGTTGGCGG GTATCATGTG CTGGAGGGTC ATGGCAGAGC TGCCTCTATG
    CCCAGACTGA ATGCTGGCTT CCAGCGGAGC CACTTACGCC ACACCGCCGG AGCGTATCTA
    GCACCAATTG CAGATGTGAG TCCCATGCGG CGATCCACAT CCTCGCTGAC TCCACAAAGA
    GGTGCGCGAG AGGTCAACCT GAGCGAATAC GACCTGGAGA GACCCAGGAT GAGCCCGAGC
    CAGACTTTCG CTCAGGACAG AGAGAGAGAG AGAGTCCACC ATCATCATCA CCACCATCAT
    CATCGCTGTC AGAGACGCAG AGACAAGAAG CACAAGTCAC TGGATCGAGC CATCAGCGAG
    GAGCAGCCGC CACCTGCAGC TGATCCAAAC ACAGAGTCGA CGCCCAGGGA ACGAGCCCGG
    GAGCGAGACC GTGGCCGCTC ACACGAGAGG AAGCACCACT CATCCTCAGC AGCAGAAAGG
    AAGCAGCGAT ACTACTCTTG TGACCGCTAC GGCAGCCATG AACATGGCCA CAGCCTGTCC
    GCCGGGCCTA GCCGATCCAC CTCTCCTGGA GAACCACATG ACAGGCTTAA ACAGGGAATT
    AACGCAGCTA AAGGAAGCTC AGTGGGTCAT ACCTCAGGCA CCAGCACACC CTGCCGCGGG
    CGTAGACAGC TTCCCCAGAC ACCTCTTACC CCTCGGCCTG CTGTTGCCTA TAAGACTGCC
    AACTCTTCTC CGGTCCAGCT CAACACTGCC CAATCTACAG GGCCCTGCCC CGCTCGCCTT
    AGTCGTGGCC TGTCAGAGCA CGATACCTTA CTCAGCGGTA GCATCCACCG CCAATCCCCT
    GTGCCTGTCA CTCGTATTGG CTCTGACCCC AGCTTAGGTC CCCTCCAGCA AGACTCGCAC
    TTGTCTGAAG CGGAAGACTT TCACGATGCC TTGAGCACGT ACGGCACCGG TCGCTCCCCT
    AGAACTACCG CCATCATCGC CCACACGTCA TCTGGTGCGA CCGCCACTTC TACGCTGCTG
    TCCCGAAGCC AGAGCGGAGT CCCCAACGGG TACCACTACT CCCTCGGAGT GAACGCGGCT
    CCGGGATTGA GTGGGAGAGC GTCTGGAAGC TACCAGGAGA CTGAGAGAGA TGACTGGTGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208480.1
    CDS87..6749
    Translation

    Target ORF information:

    RefSeq Version XM_017352991.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
    GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC
    TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG
    TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA
    ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG
    ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC
    ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT
    CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC
    TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA
    GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC
    GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC
    ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG
    GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC
    GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC
    GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT
    CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC
    GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC
    CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC
    TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG
    GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG
    GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG
    ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG
    GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT
    GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG
    AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG
    CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG
    GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC
    ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC
    ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT
    ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT
    GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT
    CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC
    TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC
    AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG
    CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA
    TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG
    ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT
    GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT
    AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA
    AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC
    CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA
    CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT
    GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA
    AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG
    AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC
    CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG
    GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC
    ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT
    GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT
    GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG
    ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG
    CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG
    TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT
    TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT
    TTCGCCTGCT CGAGCTTCAT GGGAGGAACT AAAGGAAAGG ACATAAACAC CATCAAGTCC
    CTGAGGGTTC TTCGAGTCTT GAGACCCTTG AAGACAATCA AAAGATTGCC CAAACTCAAG
    GCCGTGTTTG ACTGCGTGGT GAACTCTCTG AAGAATGTGC TCAACATCCT CATCGTCTAC
    ATCCTCTTCA TGTTCATCTT CGCGGTCATC GCCGTGCAGC TTTTCAAGGG GAAGTTTTTC
    CACTGCACCG ATGAGTCCAA AGCCCTGGAG AAGGACTGCA GGGGTCAGTT CTTGGAGTAC
    GGCAGTGATG GAGTGGCCAT GACGCAGCCG CGCGAGTGGA AGAAGTACGA CTTCCATTAT
    GACAACGTCC TGTGGGCCTT TCTGACCCTC TTCACTGTGT CTACAGGAGA GGGCTGGCCA
    CTTGTGCTGA AACACTCTGT TGATGCCACG TATGAAGACC AGGGTCCCAG TCCAGGATAT
    CGCATGGAAA CCTCCATCTT CTATGTGGTC TACTTCATCG TCTTTCCCTT CTTCTTTGTC
    AACATCTTTG TGGCTTTGAT CATCATCACA TTCCAGGAGC AGGGGGATAA AGCCATGTCC
    GAGTGCAGCT TGGAAAAGAA CGAGCGAGCT TGTATTGACT TTGCCATCAA TGCTAAACCT
    CTGACTCGCT ACATGCCGCA AGACAAGAAC TCTTACCAGT ACAAGATGTG GAAGTTTGTG
    GTGTCGACTC CGTTTGAGTA CTCCATCCTG ACCATGATCG CCATCAATAC AGTGGTGCTC
    ATGATGAAGT TCCATGGTGC TCCTAAACCT TATGAGGACA TGTTGAAGTG GCTGAACATC
    ATCTTCACTG CACTCTTCAC ACTGGAGTGT GTTCTCAAGG TCATCGCCTT CGGTCCTCTG
    AACTACCTGA AAGAGGCTTG GAACATTTTT GACTTTGTTA CTGTTCTGGG AAGCATCACA
    GACATCCTGG TTACTGAGAT CAAGACGGAT AAGAGGATTA ATCTGAGTTT CCTGAGACTC
    TTCCGGGCGG CTCGTCTCAT TAAACTCCTT CGGCAAGGAT ACACCATTCG GATCCTTCTC
    TGGACCTTTG TTCAGTCCTT TAAGGCTCTT CCATATGTGT GTCTCCTTAT CGCAATGCTT
    TTTTTCATTT ACGCCATCAT CGGGATGCAG GTGTTTGGTA ACATCGCGTT GAACAATGAA
    GATTCGGCCA TCAACATTCA CAATAACTTC CGCACCTTTT TTCAGGCCCT CATGTTGTTG
    TTCAGAAGTG CTACAGGGGA GGCTTGGCAC GAGATCATGT TGGCTTGTCT GAGTGAAAGA
    TATTGCGATA TAGAATCTGG CTACAGTGGG AAAGAGTGCG GCAGCGATTT TGCATACTTC
    TACTTCGTCT CCTTCATCTT TCTTTGCTCG TTTCTGATGC TGAATCTGTT TGTGGCTGTC
    ATCATGGATA ATTTTGAATA TCTGACACGA GATGCTTCTA TTCTGGGGCC TCATCACCTA
    GATGAGTTCA TCCGCGTCTG GGCTGAGTAC GACCCTGCCG CCTGTGGAAG GATTCCCTAT
    ATGGATATGT ACAATTTGTT GCGTGTAATC TCACCTCCCC TTGGCTTAGG AAAGAACTGC
    CCTAATAGGG TGGCATACAA GCGATTGGTG AGGATGAACA TGCCCATAGC CGAGGACAAC
    ACGGTTCACT TTACCTCCAC TTTAATGGCT CTCATCCGGA CTGCCCTGGA GATCAAACTG
    GCATCAGGAG TTCTGGCTCA GCGCATTTGT GATGCTGAAT TAAGGAAGGA AATCAATAAA
    ATTTGGCCCA GTTTGTCATC TAAAACAGTA GACCTGCTGG TTACACCTCA TAAACATAAC
    GAATTGACTG TCGGGAAGGT CTATGCAGCG CTCATGATTT TTGACTACTA TAAGCAGAAC
    AGAGCCAAAA GACTCCAACA GCAGCAAGCT GCACCAGGCA CACAGAGTAA AGTCGGTGTT
    CTCTTCAGGC CGATGCTTCC TCTCACACAC CTGCATGACC AGGAGCCTTT AGTGCCAAAA
    GGCCCGCCTC CTTCTCATTT AGATATCACC CCTCAGTCAG ATGCCACACC CACTTCTTTT
    CACTTGACCA ATGGGGATGC AGTTCAAAGC CAGAGCAGTG CCATGAAAAA GTCTCCTTCG
    GGAGGCCCAT CTCAGGAAGT CACACAGGAA ACGAACAGAA GGCCTATCCA ACGTGGTCAA
    TCGGAAGACG TGCCAAACAC TTACAGATCT CAGGAACTAG TAGAGATGAC TGACATGGAG
    AATGATTCGG ATGTTGGCGG GTATCATGTG CTGGAGGGTC ATGGCAGAGC TGCCTCTATG
    CCCAGACTGA ATGCTGGCTT CCAGCGGAGC CACTTACGCC ACACCGCCGG AGCGTATCTA
    GCACCAATTG CAGATGTGAG TCCCATGCGG CGATCCACAT CCTCGCTGAC TCCACAAAGA
    GGTGCGCGAG AGGTCAACCT GAGCGAATAC GACCTGGAGA GACCCAGGAT GAGCCCGAGC
    CAGACTTTCG CTCAGGACAG AGAGAGAGAG AGAGTCCACC ATCATCATCA CCACCATCAT
    CATCGCTGTC AGAGACGCAG AGACAAGAAG CACAAGTCAC TGGATCGAGC CATCAGCGAG
    GAGCAGCCGC CACCTGCAGC TGATCCAAAC ACAGAGTCGA CGCCCAGGGA ACGAGCCCGG
    GAGCGAGACC GTGGCCGCTC ACACGAGAGG AAGCACCACT CATCCTCAGC AGCAGAAAGG
    AAGCAGCGAT ACTACTCTTG TGACCGCTAC GGCAGCCATG AACATGGCCA CAGCCTGTCC
    GCCGGGCCTA GCCGATCCAC CTCTCCTGGA GAACCACATG ACAGGCTTAA ACAGGGAATT
    AACGCAGCTA AAGGAAGCTC AGTGGGTCAT ACCTCAGGCA CCAGCACACC CTGCCGCGGG
    CGTAGACAGC TTCCCCAGAC ACCTCTTACC CCTCGGCCTG CTGTTGCCTA TAAGACTGCC
    AACTCTTCTC CGGTCCAGCT CAACACTGCC CAATCTACAG GGCCCTGCCC CGCTCGCCTT
    AGTCGTGGCC TGTCAGAGCA CGATACCTTA CTCAGCGGTA GCATCCACCG CCAATCCCCT
    GTGCCTGTCA CTCGTATTGG CTCTGACCCC AGCTTAGGTC CCCTCCAGCA AGACTCGCAC
    TTGTCTGAAG CGGAAGACTT TCACGATGCC TTGAGCACGT ACGGCACCGG TCGCTCCCCT
    AGAACTACCG CCATCATCGC CCACACGTCA TCTGGTGCGA CCGCCACTTC TACGCTGCTG
    TCCCGAAGCC AGAGCGGAGT CCCCAACGGG TACCACTACT CCCTCGGAGT GAACGCGGCT
    CCGGGATTGA GTGGGAGAGC GTCTGGAAGC TACCAGGAGA CTGAGAGAGA TGACTGGTGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56941
    Clone ID Related Accession (Same CDS sequence) XM_017352992.1
    Accession Version XM_017352992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
    GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC
    TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG
    TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA
    ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG
    ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC
    ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT
    CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC
    TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA
    GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC
    GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC
    ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG
    GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC
    GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC
    GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT
    CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC
    GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC
    CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC
    TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG
    GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG
    GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG
    ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG
    GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT
    GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG
    AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG
    CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG
    GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC
    ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC
    ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT
    ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT
    GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT
    CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC
    TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC
    AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG
    CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA
    TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG
    ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT
    GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT
    AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA
    AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC
    CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA
    CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT
    GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA
    AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG
    AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC
    CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG
    GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC
    ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT
    GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT
    GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG
    ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG
    CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG
    TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT
    TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT
    TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT
    CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC
    TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG
    TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT
    GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA
    GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG
    TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA
    CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC
    TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG
    GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG
    GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC
    ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG
    TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC
    CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA
    CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA
    GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT
    ACTGAGATCA AGACGGATAA GAGGATTAAT CTGAGTTTCC TGAGACTCTT CCGGGCGGCT
    CGTCTCATTA AACTCCTTCG GCAAGGATAC ACCATTCGGA TCCTTCTCTG GACCTTTGTT
    CAGTCCTTTA AGGCTCTTCC ATATGTGTGT CTCCTTATCG CAATGCTTTT TTTCATTTAC
    GCCATCATCG GGATGCAGGT GTTTGGTAAC ATCGCGTTGA ACAATGAAGA TTCGGCCATC
    AACATTCACA ATAACTTCCG CACCTTTTTT CAGGCCCTCA TGTTGTTGTT CAGAAGTGCT
    ACAGGGGAGG CTTGGCACGA GATCATGTTG GCTTGTCTGA GTGAAAGATA TTGCGATATA
    GAATCTGGCT ACAGTGGGAA AGAGTGCGGC AGCGATTTTG CATACTTCTA CTTCGTCTCC
    TTCATCTTTC TTTGCTCGTT TCTGATGCTG AATCTGTTTG TGGCTGTCAT CATGGATAAT
    TTTGAATATC TGACACGAGA TGCTTCTATT CTGGGGCCTC ATCACCTAGA TGAGTTCATC
    CGCGTCTGGG CTGAGTACGA CCCTGCCGCC TGTGGACGCA TGTCCTACCT CGATATGTAC
    GAGATGCTCC GCCACATGTC CCCTCCGCTA GGTTTGGGAA AGAAATGTCC TCCACGAATC
    GCCTACAAGC GATTGGTGAG GATGAACATG CCCATAGCCG AGGACAACAC GGTTCACTTT
    ACCTCCACTT TAATGGCTCT CATCCGGACT GCCCTGGAGA TCAAACTGGC ATCAGGAGTT
    CTGGCTCAGC GCATTTGTGA TGCTGAATTA AGGAAGGAAA TCAATAAAAT TTGGCCCAGT
    TTGTCATCTA AAACAGTAGA CCTGCTGGTT ACACCTCATA AACATAACGA ATTGACTGTC
    GGGAAGGTCT ATGCAGCGCT CATGATTTTT GACTACTATA AGCAGAACAG AGCCAAAAGA
    CTCCAACAGC AGCAAGCTGC ACCAGGCACA CAGAGTAAAG TCGGTGTTCT CTTCAGGCCG
    ATGCTTCCTC TCACACACCT GCATGACCAG GAGCCTTTAG TGCCAAAAGG CCCGCCTCCT
    TCTCATTTAG ATATCACCCC TCAGTCAGAT GCCACACCCA CTTCTTTTCA CTTGACCAAT
    GGGGATGCAG TTCAAAGCCA GAGCAGTGCC ATGAAAAAGT CTCCTTCGGG AGGCCCATCT
    CAGGAAGTCA CACAGGAAAC GAACAGAAGG CCTATCCAAC GTGGTCAATC GGAAGACGTG
    CCAAACACTT ACAGATCTCA GGAACTAGTA GAGATGACTG ACATGGAGAA TGATTCGGAT
    GTTGGCGGGT ATCATGTGCT GGAGGGTCAT GGCAGAGCTG CCTCTATGCC CAGACTGAAT
    GCTGGCTTCC AGCGGAGCCA CTTACGCCAC ACCGCCGGAG CGTATCTAGC ACCAATTGCA
    GATGTGAGTC CCATGCGGCG ATCCACATCC TCGCTGACTC CACAAAGAGG TGCGCGAGAG
    GTCAACCTGA GCGAATACGA CCTGGAGAGA CCCAGGATGA GCCCGAGCCA GACTTTCGCT
    CAGGACAGAG AGAGAGAGAG AGTCCACCAT CATCATCACC ACCATCATCA TCGCTGTCAG
    AGACGCAGAG ACAAGAAGCA CAAGTCACTG GATCGAGCCA TCAGCGAGGA GCAGCCGCCA
    CCTGCAGCTG ATCCAAACAC AGAGTCGACG CCCAGGGAAC GAGCCCGGGA GCGAGACCGT
    GGCCGCTCAC ACGAGAGGAA GCACCACTCA TCCTCAGCAG CAGAAAGGAA GCAGCGATAC
    TACTCTTGTG ACCGCTACGG CAGCCATGAA CATGGCCACA GCCTGTCCGC CGGGCCTAGC
    CGATCCACCT CTCCTGGAGA ACCACATGAC AGGCTTAAAC AGGGAATTAA CGCAGCTAAA
    GGAAGCTCAG TGGGTCATAC CTCAGGCACC AGCACACCCT GCCGCGGGCG TAGACAGCTT
    CCCCAGACAC CTCTTACCCC TCGGCCTGCT GTTGCCTATA AGACTGCCAA CTCTTCTCCG
    GTCCAGCTCA ACACTGCCCA ATCTACAGGG CCCTGCCCCG CTCGCCTTAG TCGTGGCCTG
    TCAGAGCACG ATACCTTACT CAGCGGTAGC ATCCACCGCC AATCCCCTGT GCCTGTCACT
    CGTATTGGCT CTGACCCCAG CTTAGGTCCC CTCCAGCAAG ACTCGCACTT GTCTGAAGCG
    GAAGACTTTC ACGATGCCTT GAGCACGTAC GGCACCGGTC GCTCCCCTAG AACTACCGCC
    ATCATCGCCC ACACGTCATC TGGTGCGACC GCCACTTCTA CGCTGCTGTC CCGAAGCCAG
    AGCGGAGTCC CCAACGGGTA CCACTACTCC CTCGGAGTGA ACGCGGCTCC GGGATTGAGT
    GGGAGAGCGT CTGGAAGCTA CCAGGAGACT GAGAGAGATG ACTGGTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208481.1
    CDS87..6737
    Translation

    Target ORF information:

    RefSeq Version XM_017352992.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
    GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC
    TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG
    TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA
    ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG
    ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC
    ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT
    CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC
    TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA
    GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC
    GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC
    ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG
    GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC
    GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC
    GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT
    CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC
    GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC
    CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC
    TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG
    GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG
    GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG
    ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG
    GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT
    GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG
    AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG
    CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG
    GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC
    ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC
    ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT
    ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT
    GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT
    CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC
    TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC
    AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG
    CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA
    TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG
    ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT
    GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT
    AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA
    AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC
    CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA
    CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT
    GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA
    AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG
    AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC
    CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG
    GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC
    ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT
    GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT
    GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG
    ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG
    CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG
    TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT
    TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT
    TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT
    CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC
    TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG
    TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT
    GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA
    GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG
    TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA
    CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC
    TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG
    GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG
    GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC
    ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG
    TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC
    CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA
    CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA
    GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT
    ACTGAGATCA AGACGGATAA GAGGATTAAT CTGAGTTTCC TGAGACTCTT CCGGGCGGCT
    CGTCTCATTA AACTCCTTCG GCAAGGATAC ACCATTCGGA TCCTTCTCTG GACCTTTGTT
    CAGTCCTTTA AGGCTCTTCC ATATGTGTGT CTCCTTATCG CAATGCTTTT TTTCATTTAC
    GCCATCATCG GGATGCAGGT GTTTGGTAAC ATCGCGTTGA ACAATGAAGA TTCGGCCATC
    AACATTCACA ATAACTTCCG CACCTTTTTT CAGGCCCTCA TGTTGTTGTT CAGAAGTGCT
    ACAGGGGAGG CTTGGCACGA GATCATGTTG GCTTGTCTGA GTGAAAGATA TTGCGATATA
    GAATCTGGCT ACAGTGGGAA AGAGTGCGGC AGCGATTTTG CATACTTCTA CTTCGTCTCC
    TTCATCTTTC TTTGCTCGTT TCTGATGCTG AATCTGTTTG TGGCTGTCAT CATGGATAAT
    TTTGAATATC TGACACGAGA TGCTTCTATT CTGGGGCCTC ATCACCTAGA TGAGTTCATC
    CGCGTCTGGG CTGAGTACGA CCCTGCCGCC TGTGGACGCA TGTCCTACCT CGATATGTAC
    GAGATGCTCC GCCACATGTC CCCTCCGCTA GGTTTGGGAA AGAAATGTCC TCCACGAATC
    GCCTACAAGC GATTGGTGAG GATGAACATG CCCATAGCCG AGGACAACAC GGTTCACTTT
    ACCTCCACTT TAATGGCTCT CATCCGGACT GCCCTGGAGA TCAAACTGGC ATCAGGAGTT
    CTGGCTCAGC GCATTTGTGA TGCTGAATTA AGGAAGGAAA TCAATAAAAT TTGGCCCAGT
    TTGTCATCTA AAACAGTAGA CCTGCTGGTT ACACCTCATA AACATAACGA ATTGACTGTC
    GGGAAGGTCT ATGCAGCGCT CATGATTTTT GACTACTATA AGCAGAACAG AGCCAAAAGA
    CTCCAACAGC AGCAAGCTGC ACCAGGCACA CAGAGTAAAG TCGGTGTTCT CTTCAGGCCG
    ATGCTTCCTC TCACACACCT GCATGACCAG GAGCCTTTAG TGCCAAAAGG CCCGCCTCCT
    TCTCATTTAG ATATCACCCC TCAGTCAGAT GCCACACCCA CTTCTTTTCA CTTGACCAAT
    GGGGATGCAG TTCAAAGCCA GAGCAGTGCC ATGAAAAAGT CTCCTTCGGG AGGCCCATCT
    CAGGAAGTCA CACAGGAAAC GAACAGAAGG CCTATCCAAC GTGGTCAATC GGAAGACGTG
    CCAAACACTT ACAGATCTCA GGAACTAGTA GAGATGACTG ACATGGAGAA TGATTCGGAT
    GTTGGCGGGT ATCATGTGCT GGAGGGTCAT GGCAGAGCTG CCTCTATGCC CAGACTGAAT
    GCTGGCTTCC AGCGGAGCCA CTTACGCCAC ACCGCCGGAG CGTATCTAGC ACCAATTGCA
    GATGTGAGTC CCATGCGGCG ATCCACATCC TCGCTGACTC CACAAAGAGG TGCGCGAGAG
    GTCAACCTGA GCGAATACGA CCTGGAGAGA CCCAGGATGA GCCCGAGCCA GACTTTCGCT
    CAGGACAGAG AGAGAGAGAG AGTCCACCAT CATCATCACC ACCATCATCA TCGCTGTCAG
    AGACGCAGAG ACAAGAAGCA CAAGTCACTG GATCGAGCCA TCAGCGAGGA GCAGCCGCCA
    CCTGCAGCTG ATCCAAACAC AGAGTCGACG CCCAGGGAAC GAGCCCGGGA GCGAGACCGT
    GGCCGCTCAC ACGAGAGGAA GCACCACTCA TCCTCAGCAG CAGAAAGGAA GCAGCGATAC
    TACTCTTGTG ACCGCTACGG CAGCCATGAA CATGGCCACA GCCTGTCCGC CGGGCCTAGC
    CGATCCACCT CTCCTGGAGA ACCACATGAC AGGCTTAAAC AGGGAATTAA CGCAGCTAAA
    GGAAGCTCAG TGGGTCATAC CTCAGGCACC AGCACACCCT GCCGCGGGCG TAGACAGCTT
    CCCCAGACAC CTCTTACCCC TCGGCCTGCT GTTGCCTATA AGACTGCCAA CTCTTCTCCG
    GTCCAGCTCA ACACTGCCCA ATCTACAGGG CCCTGCCCCG CTCGCCTTAG TCGTGGCCTG
    TCAGAGCACG ATACCTTACT CAGCGGTAGC ATCCACCGCC AATCCCCTGT GCCTGTCACT
    CGTATTGGCT CTGACCCCAG CTTAGGTCCC CTCCAGCAAG ACTCGCACTT GTCTGAAGCG
    GAAGACTTTC ACGATGCCTT GAGCACGTAC GGCACCGGTC GCTCCCCTAG AACTACCGCC
    ATCATCGCCC ACACGTCATC TGGTGCGACC GCCACTTCTA CGCTGCTGTC CCGAAGCCAG
    AGCGGAGTCC CCAACGGGTA CCACTACTCC CTCGGAGTGA ACGCGGCTCC GGGATTGAGT
    GGGAGAGCGT CTGGAAGCTA CCAGGAGACT GAGAGAGATG ACTGGTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56942
    Clone ID Related Accession (Same CDS sequence) XM_017352993.1
    Accession Version XM_017352993.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
    GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC
    TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG
    TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA
    ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG
    ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC
    ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT
    CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC
    TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA
    GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC
    GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC
    ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG
    GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC
    GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC
    GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT
    CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC
    GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC
    CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC
    TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG
    GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG
    GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG
    ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG
    GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT
    GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG
    AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG
    CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG
    GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC
    ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC
    ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT
    ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT
    GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT
    CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC
    TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC
    AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG
    CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA
    TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG
    ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT
    GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT
    AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA
    AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC
    CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA
    CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT
    GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA
    AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG
    AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC
    CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG
    GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC
    ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT
    GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT
    GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG
    ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG
    CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG
    TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT
    TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT
    TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT
    CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC
    TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG
    TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT
    GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA
    GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG
    TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA
    CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC
    TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG
    GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG
    GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC
    ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG
    TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC
    CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA
    CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA
    GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT
    ACTGAGATCA AGGATAAGAG GATTAATCTG AGTTTCCTGA GACTCTTCCG GGCGGCTCGT
    CTCATTAAAC TCCTTCGGCA AGGATACACC ATTCGGATCC TTCTCTGGAC CTTTGTTCAG
    TCCTTTAAGG CTCTTCCATA TGTGTGTCTC CTTATCGCAA TGCTTTTTTT CATTTACGCC
    ATCATCGGGA TGCAGGTGTT TGGTAACATC GCGTTGAACA ATGAAGATTC GGCCATCAAC
    ATTCACAATA ACTTCCGCAC CTTTTTTCAG GCCCTCATGT TGTTGTTCAG AAGTGCTACA
    GGGGAGGCTT GGCACGAGAT CATGTTGGCT TGTCTGAGTG AAAGATATTG CGATATAGAA
    TCTGGCTACA GTGGGAAAGA GTGCGGCAGC GATTTTGCAT ACTTCTACTT CGTCTCCTTC
    ATCTTTCTTT GCTCGTTTCT GATGCTGAAT CTGTTTGTGG CTGTCATCAT GGATAATTTT
    GAATATCTGA CACGAGATGC TTCTATTCTG GGGCCTCATC ACCTAGATGA GTTCATCCGC
    GTCTGGGCTG AGTACGACCC TGCCGCCTGT GGACGCATGT CCTACCTCGA TATGTACGAG
    ATGCTCCGCC ACATGTCCCC TCCGCTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC
    TACAAGCGAT TGGTGAGGAT GAACATGCCC ATAGCCGAGG ACAACACGGT TCACTTTACC
    TCCACTTTAA TGGCTCTCAT CCGGACTGCC CTGGAGATCA AACTGGCATC AGGAGTTCTG
    GCTCAGCGCA TTTGTGATGC TGAATTAAGG AAGGAAATCA ATAAAATTTG GCCCAGTTTG
    TCATCTAAAA CAGTAGACCT GCTGGTTACA CCTCATAAAC ATAACGAATT GACTGTCGGG
    AAGGTCTATG CAGCGCTCAT GATTTTTGAC TACTATAAGC AGAACAGAGC CAAAAGACTC
    CAACAGCAGC AAGCTGCACC AGGCACACAG AGTAAAGTCG GTGTTCTCTT CAGGCCGATG
    CTTCCTCTCA CACACCTGCA TGACCAGGAG CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT
    CATTTAGATA TCACCCCTCA GTCAGATGCC ACACCCACTT CTTTTCACTT GACCAATGGG
    GATGCAGTTC AAAGCCAGAG CAGTGCCATG AAAAAGTCTC CTTCGGGAGG CCCATCTCAG
    GAAGTCACAC AGGAAACGAA CAGAAGGCCT ATCCAACGTG GTCAATCGGA AGACGTGCCA
    AACACTTACA GATCTCAGGA ACTAGTAGAG ATGACTGACA TGGAGAATGA TTCGGATGTT
    GGCGGGTATC ATGTGCTGGA GGGTCATGGC AGAGCTGCCT CTATGCCCAG ACTGAATGCT
    GGCTTCCAGC GGAGCCACTT ACGCCACACC GCCGGAGCGT ATCTAGCACC AATTGCAGAT
    GTGAGTCCCA TGCGGCGATC CACATCCTCG CTGACTCCAC AAAGAGGTGC GCGAGAGGTC
    AACCTGAGCG AATACGACCT GGAGAGACCC AGGATGAGCC CGAGCCAGAC TTTCGCTCAG
    GACAGAGAGA GAGAGAGAGT CCACCATCAT CATCACCACC ATCATCATCG CTGTCAGAGA
    CGCAGAGACA AGAAGCACAA GTCACTGGAT CGAGCCATCA GCGAGGAGCA GCCGCCACCT
    GCAGCTGATC CAAACACAGA GTCGACGCCC AGGGAACGAG CCCGGGAGCG AGACCGTGGC
    CGCTCACACG AGAGGAAGCA CCACTCATCC TCAGCAGCAG AAAGGAAGCA GCGATACTAC
    TCTTGTGACC GCTACGGCAG CCATGAACAT GGCCACAGCC TGTCCGCCGG GCCTAGCCGA
    TCCACCTCTC CTGGAGAACC ACATGACAGG CTTAAACAGG GAATTAACGC AGCTAAAGGA
    AGCTCAGTGG GTCATACCTC AGGCACCAGC ACACCCTGCC GCGGGCGTAG ACAGCTTCCC
    CAGACACCTC TTACCCCTCG GCCTGCTGTT GCCTATAAGA CTGCCAACTC TTCTCCGGTC
    CAGCTCAACA CTGCCCAATC TACAGGGCCC TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA
    GAGCACGATA CCTTACTCAG CGGTAGCATC CACCGCCAAT CCCCTGTGCC TGTCACTCGT
    ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC CAGCAAGACT CGCACTTGTC TGAAGCGGAA
    GACTTTCACG ATGCCTTGAG CACGTACGGC ACCGGTCGCT CCCCTAGAAC TACCGCCATC
    ATCGCCCACA CGTCATCTGG TGCGACCGCC ACTTCTACGC TGCTGTCCCG AAGCCAGAGC
    GGAGTCCCCA ACGGGTACCA CTACTCCCTC GGAGTGAACG CGGCTCCGGG ATTGAGTGGG
    AGAGCGTCTG GAAGCTACCA GGAGACTGAG AGAGATGACT GGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208482.1
    CDS87..6734
    Translation

    Target ORF information:

    RefSeq Version XM_017352993.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
    GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC
    TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG
    TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA
    ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG
    ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC
    ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT
    CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC
    TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA
    GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC
    GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC
    ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG
    GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC
    GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC
    GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT
    CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC
    GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC
    CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC
    TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG
    GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG
    GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG
    ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG
    GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT
    GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG
    AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG
    CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG
    GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC
    ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC
    ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT
    ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT
    GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT
    CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC
    TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC
    AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG
    CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA
    TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG
    ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT
    GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT
    AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA
    AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC
    CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA
    CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT
    GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA
    AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG
    AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC
    CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG
    GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC
    ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT
    GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT
    GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG
    ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG
    CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG
    TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT
    TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT
    TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT
    CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC
    TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG
    TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT
    GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA
    GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG
    TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA
    CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC
    TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG
    GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG
    GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC
    ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG
    TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC
    CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA
    CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA
    GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT
    ACTGAGATCA AGGATAAGAG GATTAATCTG AGTTTCCTGA GACTCTTCCG GGCGGCTCGT
    CTCATTAAAC TCCTTCGGCA AGGATACACC ATTCGGATCC TTCTCTGGAC CTTTGTTCAG
    TCCTTTAAGG CTCTTCCATA TGTGTGTCTC CTTATCGCAA TGCTTTTTTT CATTTACGCC
    ATCATCGGGA TGCAGGTGTT TGGTAACATC GCGTTGAACA ATGAAGATTC GGCCATCAAC
    ATTCACAATA ACTTCCGCAC CTTTTTTCAG GCCCTCATGT TGTTGTTCAG AAGTGCTACA
    GGGGAGGCTT GGCACGAGAT CATGTTGGCT TGTCTGAGTG AAAGATATTG CGATATAGAA
    TCTGGCTACA GTGGGAAAGA GTGCGGCAGC GATTTTGCAT ACTTCTACTT CGTCTCCTTC
    ATCTTTCTTT GCTCGTTTCT GATGCTGAAT CTGTTTGTGG CTGTCATCAT GGATAATTTT
    GAATATCTGA CACGAGATGC TTCTATTCTG GGGCCTCATC ACCTAGATGA GTTCATCCGC
    GTCTGGGCTG AGTACGACCC TGCCGCCTGT GGACGCATGT CCTACCTCGA TATGTACGAG
    ATGCTCCGCC ACATGTCCCC TCCGCTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC
    TACAAGCGAT TGGTGAGGAT GAACATGCCC ATAGCCGAGG ACAACACGGT TCACTTTACC
    TCCACTTTAA TGGCTCTCAT CCGGACTGCC CTGGAGATCA AACTGGCATC AGGAGTTCTG
    GCTCAGCGCA TTTGTGATGC TGAATTAAGG AAGGAAATCA ATAAAATTTG GCCCAGTTTG
    TCATCTAAAA CAGTAGACCT GCTGGTTACA CCTCATAAAC ATAACGAATT GACTGTCGGG
    AAGGTCTATG CAGCGCTCAT GATTTTTGAC TACTATAAGC AGAACAGAGC CAAAAGACTC
    CAACAGCAGC AAGCTGCACC AGGCACACAG AGTAAAGTCG GTGTTCTCTT CAGGCCGATG
    CTTCCTCTCA CACACCTGCA TGACCAGGAG CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT
    CATTTAGATA TCACCCCTCA GTCAGATGCC ACACCCACTT CTTTTCACTT GACCAATGGG
    GATGCAGTTC AAAGCCAGAG CAGTGCCATG AAAAAGTCTC CTTCGGGAGG CCCATCTCAG
    GAAGTCACAC AGGAAACGAA CAGAAGGCCT ATCCAACGTG GTCAATCGGA AGACGTGCCA
    AACACTTACA GATCTCAGGA ACTAGTAGAG ATGACTGACA TGGAGAATGA TTCGGATGTT
    GGCGGGTATC ATGTGCTGGA GGGTCATGGC AGAGCTGCCT CTATGCCCAG ACTGAATGCT
    GGCTTCCAGC GGAGCCACTT ACGCCACACC GCCGGAGCGT ATCTAGCACC AATTGCAGAT
    GTGAGTCCCA TGCGGCGATC CACATCCTCG CTGACTCCAC AAAGAGGTGC GCGAGAGGTC
    AACCTGAGCG AATACGACCT GGAGAGACCC AGGATGAGCC CGAGCCAGAC TTTCGCTCAG
    GACAGAGAGA GAGAGAGAGT CCACCATCAT CATCACCACC ATCATCATCG CTGTCAGAGA
    CGCAGAGACA AGAAGCACAA GTCACTGGAT CGAGCCATCA GCGAGGAGCA GCCGCCACCT
    GCAGCTGATC CAAACACAGA GTCGACGCCC AGGGAACGAG CCCGGGAGCG AGACCGTGGC
    CGCTCACACG AGAGGAAGCA CCACTCATCC TCAGCAGCAG AAAGGAAGCA GCGATACTAC
    TCTTGTGACC GCTACGGCAG CCATGAACAT GGCCACAGCC TGTCCGCCGG GCCTAGCCGA
    TCCACCTCTC CTGGAGAACC ACATGACAGG CTTAAACAGG GAATTAACGC AGCTAAAGGA
    AGCTCAGTGG GTCATACCTC AGGCACCAGC ACACCCTGCC GCGGGCGTAG ACAGCTTCCC
    CAGACACCTC TTACCCCTCG GCCTGCTGTT GCCTATAAGA CTGCCAACTC TTCTCCGGTC
    CAGCTCAACA CTGCCCAATC TACAGGGCCC TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA
    GAGCACGATA CCTTACTCAG CGGTAGCATC CACCGCCAAT CCCCTGTGCC TGTCACTCGT
    ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC CAGCAAGACT CGCACTTGTC TGAAGCGGAA
    GACTTTCACG ATGCCTTGAG CACGTACGGC ACCGGTCGCT CCCCTAGAAC TACCGCCATC
    ATCGCCCACA CGTCATCTGG TGCGACCGCC ACTTCTACGC TGCTGTCCCG AAGCCAGAGC
    GGAGTCCCCA ACGGGTACCA CTACTCCCTC GGAGTGAACG CGGCTCCGGG ATTGAGTGGG
    AGAGCGTCTG GAAGCTACCA GGAGACTGAG AGAGATGACT GGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56943
    Clone ID Related Accession (Same CDS sequence) XM_017352994.1
    Accession Version XM_017352994.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
    GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC
    TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG
    TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA
    ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG
    ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC
    ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT
    CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC
    TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA
    GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC
    GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC
    ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG
    GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC
    GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC
    GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT
    CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC
    GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC
    CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC
    TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG
    GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG
    GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG
    ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG
    GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT
    GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG
    AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG
    CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG
    GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC
    ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC
    ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT
    ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT
    GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT
    CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC
    TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC
    AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG
    CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA
    TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG
    ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT
    GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT
    AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA
    AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC
    CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA
    CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT
    GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA
    AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG
    AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC
    CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG
    GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC
    ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT
    GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT
    GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG
    ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG
    CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG
    TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT
    TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT
    TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT
    CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC
    TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG
    TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT
    GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA
    GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG
    TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA
    CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC
    TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG
    GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG
    GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC
    ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG
    TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC
    CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA
    CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA
    GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT
    ACTGAGATCA AGGATAAGAG GATTAATCTG AGTTTCCTGA GACTCTTCCG GGCGGCTCGT
    CTCATTAAAC TCCTTCGGCA AGGATACACC ATTCGGATCC TTCTCTGGAC CTTTGTTCAG
    TCCTTTAAGG CTCTTCCATA TGTGTGTCTC CTTATCGCAA TGCTTTTTTT CATTTACGCC
    ATCATCGGGA TGCAGGTGTT TGGTAACATC GCGTTGAACA ATGAAGATTC GGCCATCAAC
    ATTCACAATA ACTTCCGCAC CTTTTTTCAG GCCCTCATGT TGTTGTTCAG AAGTGCTACA
    GGGGAGGCTT GGCACGAGAT CATGTTGGCT TGTCTGAGTG AAAGATATTG CGATATAGAA
    TCTGGCTACA GTGGGAAAGA GTGCGGCAGC GATTTTGCAT ACTTCTACTT CGTCTCCTTC
    ATCTTTCTTT GCTCGTTTCT GATGCTGAAT CTGTTTGTGG CTGTCATCAT GGATAATTTT
    GAATATCTGA CACGAGATGC TTCTATTCTG GGGCCTCATC ACCTAGATGA GTTCATCCGC
    GTCTGGGCTG AGTACGACCC TGCCGCCTGT GGAAGGATTC CCTATATGGA TATGTACAAT
    TTGTTGCGTG TAATCTCACC TCCCCTTGGC TTAGGAAAGA ACTGCCCTAA TAGGGTGGCA
    TACAAGCGAT TGGTGAGGAT GAACATGCCC ATAGCCGAGG ACAACACGGT TCACTTTACC
    TCCACTTTAA TGGCTCTCAT CCGGACTGCC CTGGAGATCA AACTGGCATC AGGAGTTCTG
    GCTCAGCGCA TTTGTGATGC TGAATTAAGG AAGGAAATCA ATAAAATTTG GCCCAGTTTG
    TCATCTAAAA CAGTAGACCT GCTGGTTACA CCTCATAAAC ATAACGAATT GACTGTCGGG
    AAGGTCTATG CAGCGCTCAT GATTTTTGAC TACTATAAGC AGAACAGAGC CAAAAGACTC
    CAACAGCAGC AAGCTGCACC AGGCACACAG AGTAAAGTCG GTGTTCTCTT CAGGCCGATG
    CTTCCTCTCA CACACCTGCA TGACCAGGAG CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT
    CATTTAGATA TCACCCCTCA GTCAGATGCC ACACCCACTT CTTTTCACTT GACCAATGGG
    GATGCAGTTC AAAGCCAGAG CAGTGCCATG AAAAAGTCTC CTTCGGGAGG CCCATCTCAG
    GAAGTCACAC AGGAAACGAA CAGAAGGCCT ATCCAACGTG GTCAATCGGA AGACGTGCCA
    AACACTTACA GATCTCAGGA ACTAGTAGAG ATGACTGACA TGGAGAATGA TTCGGATGTT
    GGCGGGTATC ATGTGCTGGA GGGTCATGGC AGAGCTGCCT CTATGCCCAG ACTGAATGCT
    GGCTTCCAGC GGAGCCACTT ACGCCACACC GCCGGAGCGT ATCTAGCACC AATTGCAGAT
    GTGAGTCCCA TGCGGCGATC CACATCCTCG CTGACTCCAC AAAGAGGTGC GCGAGAGGTC
    AACCTGAGCG AATACGACCT GGAGAGACCC AGGATGAGCC CGAGCCAGAC TTTCGCTCAG
    GACAGAGAGA GAGAGAGAGT CCACCATCAT CATCACCACC ATCATCATCG CTGTCAGAGA
    CGCAGAGACA AGAAGCACAA GTCACTGGAT CGAGCCATCA GCGAGGAGCA GCCGCCACCT
    GCAGCTGATC CAAACACAGA GTCGACGCCC AGGGAACGAG CCCGGGAGCG AGACCGTGGC
    CGCTCACACG AGAGGAAGCA CCACTCATCC TCAGCAGCAG AAAGGAAGCA GCGATACTAC
    TCTTGTGACC GCTACGGCAG CCATGAACAT GGCCACAGCC TGTCCGCCGG GCCTAGCCGA
    TCCACCTCTC CTGGAGAACC ACATGACAGG CTTAAACAGG GAATTAACGC AGCTAAAGGA
    AGCTCAGTGG GTCATACCTC AGGCACCAGC ACACCCTGCC GCGGGCGTAG ACAGCTTCCC
    CAGACACCTC TTACCCCTCG GCCTGCTGTT GCCTATAAGA CTGCCAACTC TTCTCCGGTC
    CAGCTCAACA CTGCCCAATC TACAGGGCCC TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA
    GAGCACGATA CCTTACTCAG CGGTAGCATC CACCGCCAAT CCCCTGTGCC TGTCACTCGT
    ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC CAGCAAGACT CGCACTTGTC TGAAGCGGAA
    GACTTTCACG ATGCCTTGAG CACGTACGGC ACCGGTCGCT CCCCTAGAAC TACCGCCATC
    ATCGCCCACA CGTCATCTGG TGCGACCGCC ACTTCTACGC TGCTGTCCCG AAGCCAGAGC
    GGAGTCCCCA ACGGGTACCA CTACTCCCTC GGAGTGAACG CGGCTCCGGG ATTGAGTGGG
    AGAGCGTCTG GAAGCTACCA GGAGACTGAG AGAGATGACT GGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208483.1
    CDS87..6734
    Translation

    Target ORF information:

    RefSeq Version XM_017352994.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352994.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
    GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC
    TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG
    TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA
    ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG
    ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC
    ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT
    CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC
    TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA
    GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC
    GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC
    ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG
    GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC
    GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC
    GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT
    CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC
    GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC
    CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC
    TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG
    GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG
    GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG
    ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG
    GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT
    GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG
    AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG
    CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG
    GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC
    ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC
    ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT
    ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT
    GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT
    CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC
    TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC
    AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG
    CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA
    TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG
    ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT
    GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT
    AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA
    AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC
    CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA
    CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT
    GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA
    AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG
    AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC
    CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG
    GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC
    ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT
    GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT
    GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG
    ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG
    CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG
    TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT
    TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT
    TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT
    CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC
    TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG
    TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT
    GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA
    GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG
    TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA
    CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC
    TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG
    GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG
    GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC
    ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG
    TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC
    CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA
    CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA
    GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT
    ACTGAGATCA AGGATAAGAG GATTAATCTG AGTTTCCTGA GACTCTTCCG GGCGGCTCGT
    CTCATTAAAC TCCTTCGGCA AGGATACACC ATTCGGATCC TTCTCTGGAC CTTTGTTCAG
    TCCTTTAAGG CTCTTCCATA TGTGTGTCTC CTTATCGCAA TGCTTTTTTT CATTTACGCC
    ATCATCGGGA TGCAGGTGTT TGGTAACATC GCGTTGAACA ATGAAGATTC GGCCATCAAC
    ATTCACAATA ACTTCCGCAC CTTTTTTCAG GCCCTCATGT TGTTGTTCAG AAGTGCTACA
    GGGGAGGCTT GGCACGAGAT CATGTTGGCT TGTCTGAGTG AAAGATATTG CGATATAGAA
    TCTGGCTACA GTGGGAAAGA GTGCGGCAGC GATTTTGCAT ACTTCTACTT CGTCTCCTTC
    ATCTTTCTTT GCTCGTTTCT GATGCTGAAT CTGTTTGTGG CTGTCATCAT GGATAATTTT
    GAATATCTGA CACGAGATGC TTCTATTCTG GGGCCTCATC ACCTAGATGA GTTCATCCGC
    GTCTGGGCTG AGTACGACCC TGCCGCCTGT GGAAGGATTC CCTATATGGA TATGTACAAT
    TTGTTGCGTG TAATCTCACC TCCCCTTGGC TTAGGAAAGA ACTGCCCTAA TAGGGTGGCA
    TACAAGCGAT TGGTGAGGAT GAACATGCCC ATAGCCGAGG ACAACACGGT TCACTTTACC
    TCCACTTTAA TGGCTCTCAT CCGGACTGCC CTGGAGATCA AACTGGCATC AGGAGTTCTG
    GCTCAGCGCA TTTGTGATGC TGAATTAAGG AAGGAAATCA ATAAAATTTG GCCCAGTTTG
    TCATCTAAAA CAGTAGACCT GCTGGTTACA CCTCATAAAC ATAACGAATT GACTGTCGGG
    AAGGTCTATG CAGCGCTCAT GATTTTTGAC TACTATAAGC AGAACAGAGC CAAAAGACTC
    CAACAGCAGC AAGCTGCACC AGGCACACAG AGTAAAGTCG GTGTTCTCTT CAGGCCGATG
    CTTCCTCTCA CACACCTGCA TGACCAGGAG CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT
    CATTTAGATA TCACCCCTCA GTCAGATGCC ACACCCACTT CTTTTCACTT GACCAATGGG
    GATGCAGTTC AAAGCCAGAG CAGTGCCATG AAAAAGTCTC CTTCGGGAGG CCCATCTCAG
    GAAGTCACAC AGGAAACGAA CAGAAGGCCT ATCCAACGTG GTCAATCGGA AGACGTGCCA
    AACACTTACA GATCTCAGGA ACTAGTAGAG ATGACTGACA TGGAGAATGA TTCGGATGTT
    GGCGGGTATC ATGTGCTGGA GGGTCATGGC AGAGCTGCCT CTATGCCCAG ACTGAATGCT
    GGCTTCCAGC GGAGCCACTT ACGCCACACC GCCGGAGCGT ATCTAGCACC AATTGCAGAT
    GTGAGTCCCA TGCGGCGATC CACATCCTCG CTGACTCCAC AAAGAGGTGC GCGAGAGGTC
    AACCTGAGCG AATACGACCT GGAGAGACCC AGGATGAGCC CGAGCCAGAC TTTCGCTCAG
    GACAGAGAGA GAGAGAGAGT CCACCATCAT CATCACCACC ATCATCATCG CTGTCAGAGA
    CGCAGAGACA AGAAGCACAA GTCACTGGAT CGAGCCATCA GCGAGGAGCA GCCGCCACCT
    GCAGCTGATC CAAACACAGA GTCGACGCCC AGGGAACGAG CCCGGGAGCG AGACCGTGGC
    CGCTCACACG AGAGGAAGCA CCACTCATCC TCAGCAGCAG AAAGGAAGCA GCGATACTAC
    TCTTGTGACC GCTACGGCAG CCATGAACAT GGCCACAGCC TGTCCGCCGG GCCTAGCCGA
    TCCACCTCTC CTGGAGAACC ACATGACAGG CTTAAACAGG GAATTAACGC AGCTAAAGGA
    AGCTCAGTGG GTCATACCTC AGGCACCAGC ACACCCTGCC GCGGGCGTAG ACAGCTTCCC
    CAGACACCTC TTACCCCTCG GCCTGCTGTT GCCTATAAGA CTGCCAACTC TTCTCCGGTC
    CAGCTCAACA CTGCCCAATC TACAGGGCCC TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA
    GAGCACGATA CCTTACTCAG CGGTAGCATC CACCGCCAAT CCCCTGTGCC TGTCACTCGT
    ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC CAGCAAGACT CGCACTTGTC TGAAGCGGAA
    GACTTTCACG ATGCCTTGAG CACGTACGGC ACCGGTCGCT CCCCTAGAAC TACCGCCATC
    ATCGCCCACA CGTCATCTGG TGCGACCGCC ACTTCTACGC TGCTGTCCCG AAGCCAGAGC
    GGAGTCCCCA ACGGGTACCA CTACTCCCTC GGAGTGAACG CGGCTCCGGG ATTGAGTGGG
    AGAGCGTCTG GAAGCTACCA GGAGACTGAG AGAGATGACT GGTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.