phka1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	phka1b
Entrez Gene ID	562402
Full Name	phosphorylase kinase, alpha 1b (muscle)
Synonyms	si:dkey-22f5.10
General protein information	Preferred Names phosphorylase kinase, alpha 1b (muscle) Names phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like si:dkey-22f5.10
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009295578.2	XP_009293853.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like
XM_009295578.3	XP_009293853.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like isoform X1
XM_021469189.1	XP_021324864.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like isoform X2
XM_021469190.1	XP_021324865.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like isoform X3
XM_021469191.1	XP_021324866.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like isoform X4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	phka1	XP_005166575.1
Pan troglodytes (chimpanzee)	PHKA1	XP_003317571.2
Mus musculus (house mouse)	Phka1	NP_032858.2
Gallus gallus (chicken)	PHKA1	XP_004940655.1
	PHKA1	XP_001094193.1
Xenopus tropicalis (tropical clawed frog)	phka1	NP_001121510.1
Homo sapiens (human)	PHKA1	NP_002628.2
Canis lupus familiaris (dog)	PHKA1	XP_005641551.1
Bos taurus (cattle)	PHKA1	XP_005228049.1
Rattus norvegicus (Norway rat)	Phka1	NP_072148.1
Danio rerio (zebrafish)	si:dkey-22f5.10	XP_690880.4

Gene Ontology

The following phka1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phka1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151101	XM_021469191.1 Latest version!	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa43811	XM_021469189.1 Latest version!	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa43812	XM_021469190.1 Latest version!	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa43809	XM_009295578.3 Latest version!	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa43809	XM_009295578.2	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phka1b ( XM_021469191.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324866.1 Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa151101
Clone ID Related Accession (Same CDS sequence)	XM_021469191.1
Accession Version	XM_021469191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGAAGTGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324866.1
CDS	79..1881
Translation	MRSRSNSGVKLDNYARTLQQTILCQQDPVTGLLPGDEKLPHAWVRDNVYCILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQLDKVEKFKYSKSTKDCLHAKYHTGTCATVVGDQEWGHLQVDATSIFLLFLGQMTASGLNIVYTRDEVDVVQNLIFYIESAYKVADFGMWERGDKTNKGIPEINASSIGMAKAALEALDGLNLFGAKGGPESVVHALADDIQHCNSILNSMLPRASTSKEVDAGVLSIISYPAFAVEDIDVVNITKEEIISKLQGRYGCCRFLRDGHKTPREDPNRLHYESSELKLFENIECEWPLFWTYLILDGIFIKSPEQVQEYREALDGILIRGKNGLRLVPELYSVPLDKVDEEYVNPHTVERAPVGKCPLKWGQSLYILGNLLAEGFLAPGEIDPLNRRLSTIPKPDVVVQVSILAENDEIKHLLQGHGIDSETLEDIHPIRVQPSRVLSHIYARLGRNQRLGLTGRPYRRIGVLGTSKFYIIRDTIFTFTPQFVDHQEFYLALDNHMIVEMLRVDLSYLCSHWRMTGRPTVTFPISHGMLSDDYKQVNPTVLATLKKLQDGYFGGARSG

Target ORF information:

RefSeq Version	XM_021469191.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469191.1

ORF Insert Sequence:

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGAAGTGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1b ( XM_021469189.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324864.1 Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43811
Clone ID Related Accession (Same CDS sequence)	XM_021469189.1
Accession Version	XM_021469189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGGGTTAAG 
ACTGGAAAAC TGACTGATTT TCTTGACACC TCCTGTTGTG CTCATCTTAC TTTTATGGAC 
TGTGAAGTGG ATGACCTGGC TGTTTATCTG GACCAGCTGC TGGCAGTTCC TCAACAGCAT 
CCCATTAGTG CCACTAAAGG AGGCCTGAGC CGCTTCAGGG CAGCAGCCAG AAAGGTTCGA 
GAGATGGTGT CTCTAGTGCA CAAAGCCAGA GAGCTCAATA TACACAATGT GCATATGTAC 
CTTCCAACTA AACTCTTCCA TTCTCCGGCA CCAGACCTTA ATCTGCTGGT TGACAAAGAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324864.1
CDS	79..2184
Translation	MRSRSNSGVKLDNYARTLQQTILCQQDPVTGLLPGDEKLPHAWVRDNVYCILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQLDKVEKFKYSKSTKDCLHAKYHTGTCATVVGDQEWGHLQVDATSIFLLFLGQMTASGLNIVYTRDEVDVVQNLIFYIESAYKVADFGMWERGDKTNKGIPEINASSIGMAKAALEALDGLNLFGAKGGPESVVHALADDIQHCNSILNSMLPRASTSKEVDAGVLSIISYPAFAVEDIDVVNITKEEIISKLQGRYGCCRFLRDGHKTPREDPNRLHYESSELKLFENIECEWPLFWTYLILDGIFIKSPEQVQEYREALDGILIRGKNGLRLVPELYSVPLDKVDEEYVNPHTVERAPVGKCPLKWGQSLYILGNLLAEGFLAPGEIDPLNRRLSTIPKPDVVVQVSILAENDEIKHLLQGHGIDSETLEDIHPIRVQPSRVLSHIYARLGRNQRLGLTGRPYRRIGVLGTSKFYIIRDTIFTFTPQFVDHQEFYLALDNHMIVEMLRVDLSYLCSHWRMTGRPTVTFPISHGMLSDDYKQVNPTVLATLKKLQDGYFGGARVKTGKLTDFLDTSCCAHLTFMDCEVDDLAVYLDQLLAVPQQHPISATKGGLSRFRAAARKVREMVSLVHKARELNIHNVHMYLPTKLFHSPAPDLNLLVDKEK

Target ORF information:

RefSeq Version	XM_021469189.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469189.1

ORF Insert Sequence:

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGGGTTAAG 
ACTGGAAAAC TGACTGATTT TCTTGACACC TCCTGTTGTG CTCATCTTAC TTTTATGGAC 
TGTGAAGTGG ATGACCTGGC TGTTTATCTG GACCAGCTGC TGGCAGTTCC TCAACAGCAT 
CCCATTAGTG CCACTAAAGG AGGCCTGAGC CGCTTCAGGG CAGCAGCCAG AAAGGTTCGA 
GAGATGGTGT CTCTAGTGCA CAAAGCCAGA GAGCTCAATA TACACAATGT GCATATGTAC 
CTTCCAACTA AACTCTTCCA TTCTCCGGCA CCAGACCTTA ATCTGCTGGT TGACAAAGAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1b ( XM_021469190.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324865.1 Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa43812
Clone ID Related Accession (Same CDS sequence)	XM_021469190.1
Accession Version	XM_021469190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGGGTTAAG 
ACTGGAAAAC TGACTGATTT TCTTGACACC TCCTGTTGTG CTCATCTTAC TTTTATGGAC 
TGTGGCATAC AAGACTGTGC TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324865.1
CDS	79..1965
Translation	MRSRSNSGVKLDNYARTLQQTILCQQDPVTGLLPGDEKLPHAWVRDNVYCILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQLDKVEKFKYSKSTKDCLHAKYHTGTCATVVGDQEWGHLQVDATSIFLLFLGQMTASGLNIVYTRDEVDVVQNLIFYIESAYKVADFGMWERGDKTNKGIPEINASSIGMAKAALEALDGLNLFGAKGGPESVVHALADDIQHCNSILNSMLPRASTSKEVDAGVLSIISYPAFAVEDIDVVNITKEEIISKLQGRYGCCRFLRDGHKTPREDPNRLHYESSELKLFENIECEWPLFWTYLILDGIFIKSPEQVQEYREALDGILIRGKNGLRLVPELYSVPLDKVDEEYVNPHTVERAPVGKCPLKWGQSLYILGNLLAEGFLAPGEIDPLNRRLSTIPKPDVVVQVSILAENDEIKHLLQGHGIDSETLEDIHPIRVQPSRVLSHIYARLGRNQRLGLTGRPYRRIGVLGTSKFYIIRDTIFTFTPQFVDHQEFYLALDNHMIVEMLRVDLSYLCSHWRMTGRPTVTFPISHGMLSDDYKQVNPTVLATLKKLQDGYFGGARVKTGKLTDFLDTSCCAHLTFMDCGIQDCAN

Target ORF information:

RefSeq Version	XM_021469190.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469190.1

ORF Insert Sequence:

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGGGTTAAG 
ACTGGAAAAC TGACTGATTT TCTTGACACC TCCTGTTGTG CTCATCTTAC TTTTATGGAC 
TGTGGCATAC AAGACTGTGC TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1b ( XM_009295578.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293853.1 Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), mRNA.

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CloneID	ODa43809
Clone ID Related Accession (Same CDS sequence)	XM_009295578.2 , XM_009295578.3
Accession Version	XM_009295578.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGGGTTAAG 
ACTGGAAAAC TGACTGATTT TCTTGACACC TCCTGTTGTG CTCATCTTAC TTTTATGGAC 
TGTGAAGTGG ATGACCTGGC TGTTTATCTG GACCAGCTGC TGGCAGTTCC TCAACAGCAT 
CCCATTAGTG CCACTAAAGG AGGCCTGAGC CGCTTCAGGG CAGCAGCCAG AAAGGTTCGA 
GAGATGGTGT CTCTAGTGCA CAAAGCCAGA GAGCTCAATA TACACAATGT GCATATGTAC 
CTTCCAACTA AACTCTTCCA TTCTCCGGCA CCAGACCTTA ATCTGCTGGT TGACAAAGAG 
AAGGTGCCTG AGACGCAGAC TCCTCCGTGT CTGAGTCTGC CCAGAGACGT CAATGGCGGG 
ATCGATTACT CAGCTCTCGT GCAGATGCTG AAGCAGAATC TGAACCTGCA AGACAAGGCT 
GATATCCTCT ACATCCTTTT CAAAGACAAG GGAATAGACT GGGACACCAA GCTACACGGG 
ATAGGTTGGC CTGTAAGACG ACTGCTGAGT GATTTGTATG AAAAAGCAGG AAGCCTGAAG 
CACTGGGGCC TCATCCGGAT GATCTGCGGG ATGCTGCGCA AGAAAGTGGA AGAGCTGGAT 
GCTGCTTGCT CATACTTATT AATCCATCAG AAACATCTAA CAGTCGGCCT TCCTCCTGAA 
CCAAGAGAGA AAACTATTTC AGCGCCGATG CCTCCAGATA AGCTGGCGAG GCTGATCGAT 
GATGCCAGTG AGAACAACCT CACCATCGCC ATCCTCACTC AGGAGATCAT GGTGTTTCTG 
GGTATGATCA TTCGCACCCA GCCCAAGATC TTTAGCGAAA TGTTTCGTCT GCGCATTGGT 
CTCATTCTTC AGGTCATGGT GACTGAACTG GCCCAGTCTC TCCAATGTTC AGGTGAGGAG 
GCTACAGAGA ATTTGATGAA CATGAGCCCA TTTGAGATGA AGAACCTGCT GCTTAATATT 
CTCAGTGGCA AAGAGTTTGG AGTTCAAAAA AGCGTTCGTT CAGTAGGTAA TGCGACCACC 
TCAGAGGTCA ATATCCAAGA CAAGAGCAAG TCCAGCTCCA CCAAAACCGA AGTGAAAGTA 
TCAAGTAAAC AGGCTGATAA TATCAAACTG GCTGTGTCAG TCCACTCGCT GGATATTGGA 
AAGATTGAAT CAGGGAGATA CAGACTTCCA TCTATCGAGT CATTTCAGGG CTCATTAGGA 
GCCACACTGG TGGGTGACAG CAGACACGGC CAGTGGCTTC GGCGCAGACG TCTGGATGGA 
GCGCTAAACA GAGTGCCGGT CGGTTTCTAT CAGAATGTCT GGAAGATTCT GCAGAAGTGC 
CACGGTCTGT CCATTGAGGG CTTTGTGCTT CCATCTTCAA CCACAAGAGA GATGACTCCT 
GGAGAAATAA AGTTTGCTGT GCATGTGGAG TCCGTGCTAA ACCGTGTTGC TCAACCCGAG 
TACCGTCAGC TGCTGGTGGA GGCCATCCTG GTGCTGACCA TGTTGGCGGA GGTGGACATC 
CAGAGCGTGG GTGGTGTCAT TCAGGTGGAG AGGATCGTTC AGATGGCCAG TGACCTGTTT 
TACAATGATC AGAAAGAGCT GGGAGCTGAA GAAAATCTCC TTGAGAAGGA TCACATGACT 
GGAATCTGCA GGTTGATCTA CGACAGCGCC CCCAGCGGCC GCTTTGGGAG CATGACGTAC 
CTCTCCAAAG CAGTGGCCAG CTATGTTGAA GACTTTCTTC CCAGTGGAGC CTGTGCCCTT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293853.1
CDS	59..3604
Translation	MRSRSNSGVKLDNYARTLQQTILCQQDPVTGLLPGDEKLPHAWVRDNVYCILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQLDKVEKFKYSKSTKDCLHAKYHTGTCATVVGDQEWGHLQVDATSIFLLFLGQMTASGLNIVYTRDEVDVVQNLIFYIESAYKVADFGMWERGDKTNKGIPEINASSIGMAKAALEALDGLNLFGAKGGPESVVHALADDIQHCNSILNSMLPRASTSKEVDAGVLSIISYPAFAVEDIDVVNITKEEIISKLQGRYGCCRFLRDGHKTPREDPNRLHYESSELKLFENIECEWPLFWTYLILDGIFIKSPEQVQEYREALDGILIRGKNGLRLVPELYSVPLDKVDEEYVNPHTVERAPVGKCPLKWGQSLYILGNLLAEGFLAPGEIDPLNRRLSTIPKPDVVVQVSILAENDEIKHLLQGHGIDSETLEDIHPIRVQPSRVLSHIYARLGRNQRLGLTGRPYRRIGVLGTSKFYIIRDTIFTFTPQFVDHQEFYLALDNHMIVEMLRVDLSYLCSHWRMTGRPTVTFPISHGMLSDDYKQVNPTVLATLKKLQDGYFGGARVKTGKLTDFLDTSCCAHLTFMDCEVDDLAVYLDQLLAVPQQHPISATKGGLSRFRAAARKVREMVSLVHKARELNIHNVHMYLPTKLFHSPAPDLNLLVDKEKVPETQTPPCLSLPRDVNGGIDYSALVQMLKQNLNLQDKADILYILFKDKGIDWDTKLHGIGWPVRRLLSDLYEKAGSLKHWGLIRMICGMLRKKVEELDAACSYLLIHQKHLTVGLPPEPREKTISAPMPPDKLARLIDDASENNLTIAILTQEIMVFLGMIIRTQPKIFSEMFRLRIGLILQVMVTELAQSLQCSGEEATENLMNMSPFEMKNLLLNILSGKEFGVQKSVRSVGNATTSEVNIQDKSKSSSTKTEVKVSSKQADNIKLAVSVHSLDIGKIESGRYRLPSIESFQGSLGATLVGDSRHGQWLRRRRLDGALNRVPVGFYQNVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFAVHVESVLNRVAQPEYRQLLVEAILVLTMLAEVDIQSVGGVIQVERIVQMASDLFYNDQKELGAEENLLEKDHMTGICRLIYDSAPSGRFGSMTYLSKAVASYVEDFLPSGACALQ

Target ORF information:

RefSeq Version	XM_009295578.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295578.2

ORF Insert Sequence:

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGGGTTAAG 
ACTGGAAAAC TGACTGATTT TCTTGACACC TCCTGTTGTG CTCATCTTAC TTTTATGGAC 
TGTGAAGTGG ATGACCTGGC TGTTTATCTG GACCAGCTGC TGGCAGTTCC TCAACAGCAT 
CCCATTAGTG CCACTAAAGG AGGCCTGAGC CGCTTCAGGG CAGCAGCCAG AAAGGTTCGA 
GAGATGGTGT CTCTAGTGCA CAAAGCCAGA GAGCTCAATA TACACAATGT GCATATGTAC 
CTTCCAACTA AACTCTTCCA TTCTCCGGCA CCAGACCTTA ATCTGCTGGT TGACAAAGAG 
AAGGTGCCTG AGACGCAGAC TCCTCCGTGT CTGAGTCTGC CCAGAGACGT CAATGGCGGG 
ATCGATTACT CAGCTCTCGT GCAGATGCTG AAGCAGAATC TGAACCTGCA AGACAAGGCT 
GATATCCTCT ACATCCTTTT CAAAGACAAG GGAATAGACT GGGACACCAA GCTACACGGG 
ATAGGTTGGC CTGTAAGACG ACTGCTGAGT GATTTGTATG AAAAAGCAGG AAGCCTGAAG 
CACTGGGGCC TCATCCGGAT GATCTGCGGG ATGCTGCGCA AGAAAGTGGA AGAGCTGGAT 
GCTGCTTGCT CATACTTATT AATCCATCAG AAACATCTAA CAGTCGGCCT TCCTCCTGAA 
CCAAGAGAGA AAACTATTTC AGCGCCGATG CCTCCAGATA AGCTGGCGAG GCTGATCGAT 
GATGCCAGTG AGAACAACCT CACCATCGCC ATCCTCACTC AGGAGATCAT GGTGTTTCTG 
GGTATGATCA TTCGCACCCA GCCCAAGATC TTTAGCGAAA TGTTTCGTCT GCGCATTGGT 
CTCATTCTTC AGGTCATGGT GACTGAACTG GCCCAGTCTC TCCAATGTTC AGGTGAGGAG 
GCTACAGAGA ATTTGATGAA CATGAGCCCA TTTGAGATGA AGAACCTGCT GCTTAATATT 
CTCAGTGGCA AAGAGTTTGG AGTTCAAAAA AGCGTTCGTT CAGTAGGTAA TGCGACCACC 
TCAGAGGTCA ATATCCAAGA CAAGAGCAAG TCCAGCTCCA CCAAAACCGA AGTGAAAGTA 
TCAAGTAAAC AGGCTGATAA TATCAAACTG GCTGTGTCAG TCCACTCGCT GGATATTGGA 
AAGATTGAAT CAGGGAGATA CAGACTTCCA TCTATCGAGT CATTTCAGGG CTCATTAGGA 
GCCACACTGG TGGGTGACAG CAGACACGGC CAGTGGCTTC GGCGCAGACG TCTGGATGGA 
GCGCTAAACA GAGTGCCGGT CGGTTTCTAT CAGAATGTCT GGAAGATTCT GCAGAAGTGC 
CACGGTCTGT CCATTGAGGG CTTTGTGCTT CCATCTTCAA CCACAAGAGA GATGACTCCT 
GGAGAAATAA AGTTTGCTGT GCATGTGGAG TCCGTGCTAA ACCGTGTTGC TCAACCCGAG 
TACCGTCAGC TGCTGGTGGA GGCCATCCTG GTGCTGACCA TGTTGGCGGA GGTGGACATC 
CAGAGCGTGG GTGGTGTCAT TCAGGTGGAG AGGATCGTTC AGATGGCCAG TGACCTGTTT 
TACAATGATC AGAAAGAGCT GGGAGCTGAA GAAAATCTCC TTGAGAAGGA TCACATGACT 
GGAATCTGCA GGTTGATCTA CGACAGCGCC CCCAGCGGCC GCTTTGGGAG CATGACGTAC 
CTCTCCAAAG CAGTGGCCAG CTATGTTGAA GACTTTCTTC CCAGTGGAGC CTGTGCCCTT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1b ( XM_009295578.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293853.1 Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43809
Clone ID Related Accession (Same CDS sequence)	XM_009295578.2 , XM_009295578.3
Accession Version	XM_009295578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGGGTTAAG 
ACTGGAAAAC TGACTGATTT TCTTGACACC TCCTGTTGTG CTCATCTTAC TTTTATGGAC 
TGTGAAGTGG ATGACCTGGC TGTTTATCTG GACCAGCTGC TGGCAGTTCC TCAACAGCAT 
CCCATTAGTG CCACTAAAGG AGGCCTGAGC CGCTTCAGGG CAGCAGCCAG AAAGGTTCGA 
GAGATGGTGT CTCTAGTGCA CAAAGCCAGA GAGCTCAATA TACACAATGT GCATATGTAC 
CTTCCAACTA AACTCTTCCA TTCTCCGGCA CCAGACCTTA ATCTGCTGGT TGACAAAGAG 
AAGGTGCCTG AGACGCAGAC TCCTCCGTGT CTGAGTCTGC CCAGAGACGT CAATGGCGGG 
ATCGATTACT CAGCTCTCGT GCAGATGCTG AAGCAGAATC TGAACCTGCA AGACAAGGCT 
GATATCCTCT ACATCCTTTT CAAAGACAAG GGAATAGACT GGGACACCAA GCTACACGGG 
ATAGGTTGGC CTGTAAGACG ACTGCTGAGT GATTTGTATG AAAAAGCAGG AAGCCTGAAG 
CACTGGGGCC TCATCCGGAT GATCTGCGGG ATGCTGCGCA AGAAAGTGGA AGAGCTGGAT 
GCTGCTTGCT CATACTTATT AATCCATCAG AAACATCTAA CAGTCGGCCT TCCTCCTGAA 
CCAAGAGAGA AAACTATTTC AGCGCCGATG CCTCCAGATA AGCTGGCGAG GCTGATCGAT 
GATGCCAGTG AGAACAACCT CACCATCGCC ATCCTCACTC AGGAGATCAT GGTGTTTCTG 
GGTATGATCA TTCGCACCCA GCCCAAGATC TTTAGCGAAA TGTTTCGTCT GCGCATTGGT 
CTCATTCTTC AGGTCATGGT GACTGAACTG GCCCAGTCTC TCCAATGTTC AGGTGAGGAG 
GCTACAGAGA ATTTGATGAA CATGAGCCCA TTTGAGATGA AGAACCTGCT GCTTAATATT 
CTCAGTGGCA AAGAGTTTGG AGTTCAAAAA AGCGTTCGTT CAGTAGGTAA TGCGACCACC 
TCAGAGGTCA ATATCCAAGA CAAGAGCAAG TCCAGCTCCA CCAAAACCGA AGTGAAAGTA 
TCAAGTAAAC AGGCTGATAA TATCAAACTG GCTGTGTCAG TCCACTCGCT GGATATTGGA 
AAGATTGAAT CAGGGAGATA CAGACTTCCA TCTATCGAGT CATTTCAGGG CTCATTAGGA 
GCCACACTGG TGGGTGACAG CAGACACGGC CAGTGGCTTC GGCGCAGACG TCTGGATGGA 
GCGCTAAACA GAGTGCCGGT CGGTTTCTAT CAGAATGTCT GGAAGATTCT GCAGAAGTGC 
CACGGTCTGT CCATTGAGGG CTTTGTGCTT CCATCTTCAA CCACAAGAGA GATGACTCCT 
GGAGAAATAA AGTTTGCTGT GCATGTGGAG TCCGTGCTAA ACCGTGTTGC TCAACCCGAG 
TACCGTCAGC TGCTGGTGGA GGCCATCCTG GTGCTGACCA TGTTGGCGGA GGTGGACATC 
CAGAGCGTGG GTGGTGTCAT TCAGGTGGAG AGGATCGTTC AGATGGCCAG TGACCTGTTT 
TACAATGATC AGAAAGAGCT GGGAGCTGAA GAAAATCTCC TTGAGAAGGA TCACATGACT 
GGAATCTGCA GGTTGATCTA CGACAGCGCC CCCAGCGGCC GCTTTGGGAG CATGACGTAC 
CTCTCCAAAG CAGTGGCCAG CTATGTTGAA GACTTTCTTC CCAGTGGAGC CTGTGCCCTT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293853.1
CDS	78..3623
Translation	MRSRSNSGVKLDNYARTLQQTILCQQDPVTGLLPGDEKLPHAWVRDNVYCILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQLDKVEKFKYSKSTKDCLHAKYHTGTCATVVGDQEWGHLQVDATSIFLLFLGQMTASGLNIVYTRDEVDVVQNLIFYIESAYKVADFGMWERGDKTNKGIPEINASSIGMAKAALEALDGLNLFGAKGGPESVVHALADDIQHCNSILNSMLPRASTSKEVDAGVLSIISYPAFAVEDIDVVNITKEEIISKLQGRYGCCRFLRDGHKTPREDPNRLHYESSELKLFENIECEWPLFWTYLILDGIFIKSPEQVQEYREALDGILIRGKNGLRLVPELYSVPLDKVDEEYVNPHTVERAPVGKCPLKWGQSLYILGNLLAEGFLAPGEIDPLNRRLSTIPKPDVVVQVSILAENDEIKHLLQGHGIDSETLEDIHPIRVQPSRVLSHIYARLGRNQRLGLTGRPYRRIGVLGTSKFYIIRDTIFTFTPQFVDHQEFYLALDNHMIVEMLRVDLSYLCSHWRMTGRPTVTFPISHGMLSDDYKQVNPTVLATLKKLQDGYFGGARVKTGKLTDFLDTSCCAHLTFMDCEVDDLAVYLDQLLAVPQQHPISATKGGLSRFRAAARKVREMVSLVHKARELNIHNVHMYLPTKLFHSPAPDLNLLVDKEKVPETQTPPCLSLPRDVNGGIDYSALVQMLKQNLNLQDKADILYILFKDKGIDWDTKLHGIGWPVRRLLSDLYEKAGSLKHWGLIRMICGMLRKKVEELDAACSYLLIHQKHLTVGLPPEPREKTISAPMPPDKLARLIDDASENNLTIAILTQEIMVFLGMIIRTQPKIFSEMFRLRIGLILQVMVTELAQSLQCSGEEATENLMNMSPFEMKNLLLNILSGKEFGVQKSVRSVGNATTSEVNIQDKSKSSSTKTEVKVSSKQADNIKLAVSVHSLDIGKIESGRYRLPSIESFQGSLGATLVGDSRHGQWLRRRRLDGALNRVPVGFYQNVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFAVHVESVLNRVAQPEYRQLLVEAILVLTMLAEVDIQSVGGVIQVERIVQMASDLFYNDQKELGAEENLLEKDHMTGICRLIYDSAPSGRFGSMTYLSKAVASYVEDFLPSGACALQ

Target ORF information:

RefSeq Version	XM_009295578.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1b (muscle) (phka1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295578.3

ORF Insert Sequence:

ATGAGAAGTC GCAGTAATTC TGGAGTCAAA CTCGACAATT ATGCCAGAAC TTTACAACAG 
ACGATCCTCT GTCAGCAGGA TCCGGTGACA GGTTTACTCC CGGGAGATGA GAAGTTACCT 
CACGCCTGGG TTCGAGACAA CGTGTACTGC ATCTTGTCAG TGTGGGCTCT GAGTTTGGCC 
TACAGGAAGA ACGCAGACAG AGATGAAGAC AAAGCCAAAG CCTATGAACT GGAGCAGAGC 
GTGGTCAAGC TCATGCGAGG ATTACTGCAG TGCATGATCA GACAGCTTGA TAAGGTGGAG 
AAGTTCAAGT ACAGCAAAAG CACTAAAGAC TGCCTTCATG CCAAGTACCA TACCGGCACA 
TGTGCCACTG TAGTCGGTGA TCAGGAATGG GGTCATCTAC AGGTGGACGC TACATCCATC 
TTTCTGCTCT TCCTGGGCCA AATGACAGCA TCAGGGCTGA ATATCGTCTA TACTCGTGAT 
GAAGTCGATG TTGTCCAGAA TCTGATTTTT TACATCGAAT CTGCATACAA AGTAGCGGAT 
TTTGGAATGT GGGAACGAGG AGACAAGACC AACAAAGGGA TCCCTGAGAT AAATGCCAGC 
TCTATCGGGA TGGCAAAGGC AGCTTTGGAG GCCTTGGACG GACTGAACCT GTTTGGTGCC 
AAAGGAGGCC CAGAGTCCGT TGTTCACGCT TTAGCTGACG ACATTCAGCA CTGCAATTCT 
ATTCTCAACT CCATGCTGCC GAGGGCATCA ACATCTAAAG AGGTGGACGC TGGAGTTCTC 
TCCATCATCT CTTATCCAGC GTTCGCAGTT GAAGACATTG ATGTTGTTAA TATCACCAAA 
GAAGAAATCA TTTCAAAGCT TCAGGGCAGA TACGGATGCT GTCGCTTTCT TCGAGATGGA 
CACAAAACTC CCAGGGAGGA TCCCAATCGA CTGCACTACG AGTCATCAGA GCTGAAGCTC 
TTCGAAAACA TAGAGTGCGA ATGGCCGCTG TTCTGGACAT ACCTCATCCT GGATGGCATT 
TTTATCAAGA GTCCAGAACA GGTCCAGGAG TACAGAGAGG CTTTGGACGG CATTCTCATC 
AGGGGAAAGA ATGGATTACG ATTGGTCCCT GAACTATATA GTGTTCCTCT GGATAAGGTT 
GATGAGGAGT ATGTAAATCC TCACACCGTG GAGAGAGCGC CAGTGGGCAA GTGTCCACTG 
AAATGGGGCC AGTCTCTGTA CATATTGGGT AACCTGCTGG CAGAGGGTTT TTTAGCACCT 
GGAGAAATCG ATCCTCTCAA CAGGCGCCTC TCCACTATCC CTAAACCTGA TGTGGTGGTT 
CAAGTGTCAA TCCTGGCAGA AAACGATGAG ATTAAACATC TCCTGCAGGG CCATGGCATT 
GACTCTGAAA CCCTGGAGGA CATTCACCCA ATCCGAGTTC AGCCCTCTAG AGTCCTCAGC 
CACATCTATG CCAGACTAGG GCGAAACCAA AGGCTGGGAC TGACTGGACG ACCGTATAGG 
CGAATTGGTG TGTTGGGAAC GTCTAAATTC TACATTATTC GCGACACCAT CTTTACCTTC 
ACACCTCAAT TTGTTGACCA TCAAGAGTTT TATCTGGCCC TGGACAATCA CATGATCGTG 
GAGATGCTGC GTGTCGACCT CTCCTACCTG TGCTCTCACT GGAGGATGAC AGGACGACCC 
ACTGTCACCT TCCCCATTTC ACACGGCATG CTCAGCGATG ATTACAAACA AGTTAATCCT 
ACGGTTTTGG CAACCCTCAA AAAATTACAA GATGGTTATT TTGGAGGAGC AAGGGTTAAG 
ACTGGAAAAC TGACTGATTT TCTTGACACC TCCTGTTGTG CTCATCTTAC TTTTATGGAC 
TGTGAAGTGG ATGACCTGGC TGTTTATCTG GACCAGCTGC TGGCAGTTCC TCAACAGCAT 
CCCATTAGTG CCACTAAAGG AGGCCTGAGC CGCTTCAGGG CAGCAGCCAG AAAGGTTCGA 
GAGATGGTGT CTCTAGTGCA CAAAGCCAGA GAGCTCAATA TACACAATGT GCATATGTAC 
CTTCCAACTA AACTCTTCCA TTCTCCGGCA CCAGACCTTA ATCTGCTGGT TGACAAAGAG 
AAGGTGCCTG AGACGCAGAC TCCTCCGTGT CTGAGTCTGC CCAGAGACGT CAATGGCGGG 
ATCGATTACT CAGCTCTCGT GCAGATGCTG AAGCAGAATC TGAACCTGCA AGACAAGGCT 
GATATCCTCT ACATCCTTTT CAAAGACAAG GGAATAGACT GGGACACCAA GCTACACGGG 
ATAGGTTGGC CTGTAAGACG ACTGCTGAGT GATTTGTATG AAAAAGCAGG AAGCCTGAAG 
CACTGGGGCC TCATCCGGAT GATCTGCGGG ATGCTGCGCA AGAAAGTGGA AGAGCTGGAT 
GCTGCTTGCT CATACTTATT AATCCATCAG AAACATCTAA CAGTCGGCCT TCCTCCTGAA 
CCAAGAGAGA AAACTATTTC AGCGCCGATG CCTCCAGATA AGCTGGCGAG GCTGATCGAT 
GATGCCAGTG AGAACAACCT CACCATCGCC ATCCTCACTC AGGAGATCAT GGTGTTTCTG 
GGTATGATCA TTCGCACCCA GCCCAAGATC TTTAGCGAAA TGTTTCGTCT GCGCATTGGT 
CTCATTCTTC AGGTCATGGT GACTGAACTG GCCCAGTCTC TCCAATGTTC AGGTGAGGAG 
GCTACAGAGA ATTTGATGAA CATGAGCCCA TTTGAGATGA AGAACCTGCT GCTTAATATT 
CTCAGTGGCA AAGAGTTTGG AGTTCAAAAA AGCGTTCGTT CAGTAGGTAA TGCGACCACC 
TCAGAGGTCA ATATCCAAGA CAAGAGCAAG TCCAGCTCCA CCAAAACCGA AGTGAAAGTA 
TCAAGTAAAC AGGCTGATAA TATCAAACTG GCTGTGTCAG TCCACTCGCT GGATATTGGA 
AAGATTGAAT CAGGGAGATA CAGACTTCCA TCTATCGAGT CATTTCAGGG CTCATTAGGA 
GCCACACTGG TGGGTGACAG CAGACACGGC CAGTGGCTTC GGCGCAGACG TCTGGATGGA 
GCGCTAAACA GAGTGCCGGT CGGTTTCTAT CAGAATGTCT GGAAGATTCT GCAGAAGTGC 
CACGGTCTGT CCATTGAGGG CTTTGTGCTT CCATCTTCAA CCACAAGAGA GATGACTCCT 
GGAGAAATAA AGTTTGCTGT GCATGTGGAG TCCGTGCTAA ACCGTGTTGC TCAACCCGAG 
TACCGTCAGC TGCTGGTGGA GGCCATCCTG GTGCTGACCA TGTTGGCGGA GGTGGACATC 
CAGAGCGTGG GTGGTGTCAT TCAGGTGGAG AGGATCGTTC AGATGGCCAG TGACCTGTTT 
TACAATGATC AGAAAGAGCT GGGAGCTGAA GAAAATCTCC TTGAGAAGGA TCACATGACT 
GGAATCTGCA GGTTGATCTA CGACAGCGCC CCCAGCGGCC GCTTTGGGAG CATGACGTAC 
CTCTCCAAAG CAGTGGCCAG CTATGTTGAA GACTTTCTTC CCAGTGGAGC CTGTGCCCTT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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phka1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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