NCKAP1 cDNA ORF clone, Danio rerio(Zebrafish)

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa02048 NM_001137665.1
Latest version!
Danio rerio NCK-associated protein 1 (nckap1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa15520 XM_005167921.4
Latest version!
Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa15521 XM_005167922.4
Latest version!
Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa15522 XM_005167923.4
Latest version!
Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa15523 XM_005167924.4
Latest version!
Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa15523 XM_005167924.3 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa15520 XM_005167921.3 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa15522 XM_005167923.3 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa15521 XM_005167922.3 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa02048
    Clone ID Related Accession (Same CDS sequence) NM_001137665.1
    Accession Version NM_001137665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX548025.9 and CR513785.10. On Nov 13, 2008 this sequence version replaced XM_001332244.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA
    AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA
    TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC
    ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC
    CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT
    GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT
    GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG
    CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT
    CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA
    GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC
    GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT
    TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC
    ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT
    GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA
    CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA
    TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC
    ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC
    AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC
    GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC
    AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC
    TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT
    TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT
    TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC
    ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC
    TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG
    TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG
    CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC
    CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC
    ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC
    AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG
    GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG
    TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC
    ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG
    ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC
    GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC
    AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA
    ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC
    GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG
    CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG
    CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA
    GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC
    AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC
    GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA
    GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT
    AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT
    TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG
    AAGTCTGGTA ACCCGGAGGA AGAGTACAAG ATCGCCTGCC TGTTGATGGT CTTCGTGGCC
    GTTTCCATGC CGACCCTGGC CAGCAACGTG ATGTCACAGT ACAGTCCCGC AATAGAAGGT
    CACTGCAACA ACATTCACTG TCTGGCTAAA GCGATAAACC AGATTGCCGC CGCTCTGTTC
    ACGATCCACA AGGGCAGCAT AGAGGATCGC CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC
    AGCCTGCTGA AGATTGGACA GGAGACGGAT AAAACCACTA CACGGAACAG AGAGTCTGTT
    TACCTGCTCC TGGACATGAT CGTTCAAGAA TCGCCATTCC TCACCATGGA CCTGTTGGAG
    TCCTGCTTTC CATACGTCCT GCTCCGAAAC GCCTATCACG CCGTATACAA GCAGTCGGTA
    TCTTCTTCAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001131137.1
    CDS217..3591
    Translation

    Target ORF information:

    RefSeq Version NM_001137665.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001137665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA
    AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA
    TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC
    ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC
    CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT
    GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT
    GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG
    CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT
    CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA
    GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC
    GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT
    TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC
    ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT
    GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA
    CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA
    TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC
    ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC
    AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC
    GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC
    AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC
    TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT
    TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT
    TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC
    ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC
    TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG
    TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG
    CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC
    CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC
    ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC
    AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG
    GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG
    TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC
    ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG
    ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC
    GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC
    AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA
    ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC
    GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG
    CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG
    CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA
    GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC
    AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC
    GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA
    GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT
    AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT
    TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG
    AAGTCTGGTA ACCCGGAGGA AGAGTACAAG ATCGCCTGCC TGTTGATGGT CTTCGTGGCC
    GTTTCCATGC CGACCCTGGC CAGCAACGTG ATGTCACAGT ACAGTCCCGC AATAGAAGGT
    CACTGCAACA ACATTCACTG TCTGGCTAAA GCGATAAACC AGATTGCCGC CGCTCTGTTC
    ACGATCCACA AGGGCAGCAT AGAGGATCGC CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC
    AGCCTGCTGA AGATTGGACA GGAGACGGAT AAAACCACTA CACGGAACAG AGAGTCTGTT
    TACCTGCTCC TGGACATGAT CGTTCAAGAA TCGCCATTCC TCACCATGGA CCTGTTGGAG
    TCCTGCTTTC CATACGTCCT GCTCCGAAAC GCCTATCACG CCGTATACAA GCAGTCGGTA
    TCTTCTTCAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15520
    Clone ID Related Accession (Same CDS sequence) XM_005167921.4 , XM_005167921.3
    Accession Version XM_005167921.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167921.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT
    TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG
    TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCAA
    CAGCTTGCAC AACTACAAAA AGAAAAGTCA GAAATACTGA AGAATTTAGC TCTATATTAC
    TTCACCTTTG TGGACGTTAT GGAATTTAAG GACCACGTTT GTGAACTGCT GAACACTATT
    GATGCTTGTC AAGTTTTCTT TGACATTACT GTAAATTTTG ACCTCACAAA GAACTACCTG
    GACTTGGTAG TGACGTACAC TACCCTGATG TTGCTGCTTT CCCGAATTGA AGAGCGCAAA
    GCGATAATCG GCCTCTACAA CTATGCCCAT GAAATGACAC ACGGTTCAAG CGATCGAGAA
    TACCCCAGGC TTGGACAGAT GATTGTGGAT TACGAGAACC CACTAAAAAA GATGATGGAG
    GAGTTTGTCC CTCATGGAAA ATCGCTGTCA GATGCGCTGG TCTCCTTGCA GATGGTGTAC
    CCACGCCGGA ATCTGTCTGC AGATCAGTGG AGAAACGCTC AGCTGCTCAG TTTGATTAGC
    GCTCCCAGCA CTATGCTCAA CCCAGCTCAA TCTGACACGA TGCCCTGTGA ATACCTATCC
    TTGGACACCA TGGAGAAATG GATCGTCTTC GGCTTCATCT TGTGTCATGC GGCTCTGAAC
    AGTGATCCTG CGGCTCTCAG TCTTTGGAAG TTGGCCCTTC AGAGCAGTAC CTGTCTCTGT
    CTGTTCAGGG ACGAGGTCTT CCACATTCAC AAAGCTGCAG AGGATCTCTT CGTAAACATT
    CGCGGTTACA ACAAACGTGT CAATGATATT CGTGAATGCA AAGAATCTGC TCTTTCACAT
    GCGGGCTCAA TGCACAGAGA ACGACGTAAG TTCTTGAGGT CTGCCCTGAA AGAGCTAGCC
    ACAGTACTGG CAGATCAGCC TGGATTGCTG GGTCCGAAGG CTCTGTTTGT GTTCATGGCA
    CTCTCCTTCG CCCGTGATGA GATCATTTGG CTGCTCAGAC ATGCCGACAA CATCCAAAAG
    AAAAGCACAG ATGACTTCAT TGACAAGCAC ATTGCAGAGT TGATCTTCTA CATGGAGGAG
    TTGAGGGCTC ACGTCCGAAA GTACGGGCCG GTGATGCAGA GGTACTATGT TCAGTACCTG
    TCCGGCTTTG ACGCGGTGGT GCTCAATGAG CTTGTACAGA ACCTGTCCGT GTGCCCAGAG
    GACGAGTCCA TCATAATGTC GTCCTTCGTG AACACTATGA CCTCTCTCAG TGTTAAACAA
    GTTGAAGATG GCGAAGTGTT TGATTTCAGA GGGATGAGGC TGGACTGGTT CAGACTTCAG
    GCCTACACCA GCGTGTCCAA GGCTTCGTTG GGACTGGCAG ATCACAGAGA ACTGGGAAAG
    ATGATGAACA CCATCATCTT CCACACTAAG ATGGTGGACT CTCTGGTGGA GATGCTTGTG
    GAGACTTCTG ACCTGTCCAT ATTCTGTTTT TACAGCCGCG CCTTTGAAAA GATGTTCCAG
    CAGTGTCTTG AACTGCCGTC CCAGTCTCGA TACTCCATCT CCTTCCCACT GCTCTGCACC
    CACTTTATGA GCTGCACTCA TGAGCTCTGC CCTGAAGAGA GGCACCACAT CGGAGACCGC
    AGCCTGTCCC TTTGCAACAT GTTCCTGGAC GAGATGGCCA AGCAAGCCCG CAACCTCATC
    ACAGACATAT GCACAGAGCA GTGCACCCTG AGCGACCAGC TACTGCCAAA ACATTGTGCC
    AAAACCATCA GTCAGGCCGT GAACAAGAAG TCGAAAAAGC AGACAGGGAA GAAGGGTGAA
    CCGGAGCGAG AAAAACCTGG CGTGGAGAGC ATGAGGAAGA ACAGACTCCT GGTCACCAAT
    CTGGATAAAC TGCACACTGC TCTGTCCGAG CTCTGCTTCT CCATCAACTA CGTCCCCAAC
    ATGATGGTCT GGGAGCACAC CTTCACCCCA CGCGAGTACC TGACCTCCCA CCTGGAGATT
    CGCTTCACTA AGTCTATAGT CGGGATGACC ATGTACAACC AGACAACACA GGAGATCGCC
    AAACCATCAG AGCTGCTCAC CAGCGTCCGG GCCTACATGA CAGTGCTGCA GTCCATTGAG
    AATTATGTGC AGATTGACAT CACCAGAGTC TTCAACAATG TGCTGCTGCA GCAAACCCAA
    CACCTGGACA GCCACGGAGA GCCAACCATC ACCAGCCTCT ACACTAACTG GTATCTGGAG
    ACTTTACTGC GGCAGGTCAG CAACGGTCAC ATCGCGTACT TTCCTGCGAT GAAAGCCTTC
    GTCAACTTGC CCACGGAGAA TGAGCTGACC TTCAACGCAG AGGAGTATTC TGACATTTCT
    GAAATGCGCT CTCTGTCGGA GCTGCTGGGT CCATATGGGA TGAAGTTCCT CAGCGAGAGT
    CTCATGTGGC ACATCTCATC CCAAGTTGCA GAACTGAAGA AACTTGTGGT GGACAATGTG
    GAGGTTTTAA CCCAGATGAG GACCAGCTTT GACAAGCCGG ACCATATGGC AGCACTCTTT
    AAGAGGCTAA CATCGGTTGA CAGCGTGCTG AAACGGATGA CCATCATTGG TGTGATTTTG
    TCGTTCCGCT CTCTGGCTCA GGAAGCTCTC CGAGATGTGC TCTCTTGCCA CATTCCCTTC
    CTGGTGAGCT CCGTTGAAGA CTTTAAAGAT CACATTCCTC GTGAAACTGA CATGAAGGTG
    GCCATGAACG TGTACGAGCT GTCTTCTGCT GCAGGCCTGC CCTGTGAGAT CGACCCTGCT
    CTAGTAGTGG CGCTGTCATC CCAGAAGTCT GAAAATATTA GCCCGGAGGA AGAGTACAAG
    ATCGCCTGCC TGTTGATGGT CTTCGTGGCC GTTTCCATGC CGACCCTGGC CAGCAACGTG
    ATGTCACAGT ACAGTCCCGC AATAGAAGGT CACTGCAACA ACATTCACTG TCTGGCTAAA
    GCGATAAACC AGATTGCCGC CGCTCTGTTC ACGATCCACA AGGGCAGCAT AGAGGATCGC
    CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC AGCCTGCTGA AGATTGGACA GGAGACGGAT
    AAAACCACTA CACGGAACAG AGAGTCTGTT TACCTGCTCC TGGACATGAT CGTTCAAGAA
    TCGCCATTCC TCACCATGGA CCTGTTGGAG TCCTGCTTTC CATACGTCCT GCTCCGAAAC
    GCCTATCACG CCGTATACAA GCAGTCGGTA TCTTCTTCAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167978.1
    CDS212..3616
    Translation

    Target ORF information:

    RefSeq Version XM_005167921.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167921.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT
    TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG
    TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCAA
    CAGCTTGCAC AACTACAAAA AGAAAAGTCA GAAATACTGA AGAATTTAGC TCTATATTAC
    TTCACCTTTG TGGACGTTAT GGAATTTAAG GACCACGTTT GTGAACTGCT GAACACTATT
    GATGCTTGTC AAGTTTTCTT TGACATTACT GTAAATTTTG ACCTCACAAA GAACTACCTG
    GACTTGGTAG TGACGTACAC TACCCTGATG TTGCTGCTTT CCCGAATTGA AGAGCGCAAA
    GCGATAATCG GCCTCTACAA CTATGCCCAT GAAATGACAC ACGGTTCAAG CGATCGAGAA
    TACCCCAGGC TTGGACAGAT GATTGTGGAT TACGAGAACC CACTAAAAAA GATGATGGAG
    GAGTTTGTCC CTCATGGAAA ATCGCTGTCA GATGCGCTGG TCTCCTTGCA GATGGTGTAC
    CCACGCCGGA ATCTGTCTGC AGATCAGTGG AGAAACGCTC AGCTGCTCAG TTTGATTAGC
    GCTCCCAGCA CTATGCTCAA CCCAGCTCAA TCTGACACGA TGCCCTGTGA ATACCTATCC
    TTGGACACCA TGGAGAAATG GATCGTCTTC GGCTTCATCT TGTGTCATGC GGCTCTGAAC
    AGTGATCCTG CGGCTCTCAG TCTTTGGAAG TTGGCCCTTC AGAGCAGTAC CTGTCTCTGT
    CTGTTCAGGG ACGAGGTCTT CCACATTCAC AAAGCTGCAG AGGATCTCTT CGTAAACATT
    CGCGGTTACA ACAAACGTGT CAATGATATT CGTGAATGCA AAGAATCTGC TCTTTCACAT
    GCGGGCTCAA TGCACAGAGA ACGACGTAAG TTCTTGAGGT CTGCCCTGAA AGAGCTAGCC
    ACAGTACTGG CAGATCAGCC TGGATTGCTG GGTCCGAAGG CTCTGTTTGT GTTCATGGCA
    CTCTCCTTCG CCCGTGATGA GATCATTTGG CTGCTCAGAC ATGCCGACAA CATCCAAAAG
    AAAAGCACAG ATGACTTCAT TGACAAGCAC ATTGCAGAGT TGATCTTCTA CATGGAGGAG
    TTGAGGGCTC ACGTCCGAAA GTACGGGCCG GTGATGCAGA GGTACTATGT TCAGTACCTG
    TCCGGCTTTG ACGCGGTGGT GCTCAATGAG CTTGTACAGA ACCTGTCCGT GTGCCCAGAG
    GACGAGTCCA TCATAATGTC GTCCTTCGTG AACACTATGA CCTCTCTCAG TGTTAAACAA
    GTTGAAGATG GCGAAGTGTT TGATTTCAGA GGGATGAGGC TGGACTGGTT CAGACTTCAG
    GCCTACACCA GCGTGTCCAA GGCTTCGTTG GGACTGGCAG ATCACAGAGA ACTGGGAAAG
    ATGATGAACA CCATCATCTT CCACACTAAG ATGGTGGACT CTCTGGTGGA GATGCTTGTG
    GAGACTTCTG ACCTGTCCAT ATTCTGTTTT TACAGCCGCG CCTTTGAAAA GATGTTCCAG
    CAGTGTCTTG AACTGCCGTC CCAGTCTCGA TACTCCATCT CCTTCCCACT GCTCTGCACC
    CACTTTATGA GCTGCACTCA TGAGCTCTGC CCTGAAGAGA GGCACCACAT CGGAGACCGC
    AGCCTGTCCC TTTGCAACAT GTTCCTGGAC GAGATGGCCA AGCAAGCCCG CAACCTCATC
    ACAGACATAT GCACAGAGCA GTGCACCCTG AGCGACCAGC TACTGCCAAA ACATTGTGCC
    AAAACCATCA GTCAGGCCGT GAACAAGAAG TCGAAAAAGC AGACAGGGAA GAAGGGTGAA
    CCGGAGCGAG AAAAACCTGG CGTGGAGAGC ATGAGGAAGA ACAGACTCCT GGTCACCAAT
    CTGGATAAAC TGCACACTGC TCTGTCCGAG CTCTGCTTCT CCATCAACTA CGTCCCCAAC
    ATGATGGTCT GGGAGCACAC CTTCACCCCA CGCGAGTACC TGACCTCCCA CCTGGAGATT
    CGCTTCACTA AGTCTATAGT CGGGATGACC ATGTACAACC AGACAACACA GGAGATCGCC
    AAACCATCAG AGCTGCTCAC CAGCGTCCGG GCCTACATGA CAGTGCTGCA GTCCATTGAG
    AATTATGTGC AGATTGACAT CACCAGAGTC TTCAACAATG TGCTGCTGCA GCAAACCCAA
    CACCTGGACA GCCACGGAGA GCCAACCATC ACCAGCCTCT ACACTAACTG GTATCTGGAG
    ACTTTACTGC GGCAGGTCAG CAACGGTCAC ATCGCGTACT TTCCTGCGAT GAAAGCCTTC
    GTCAACTTGC CCACGGAGAA TGAGCTGACC TTCAACGCAG AGGAGTATTC TGACATTTCT
    GAAATGCGCT CTCTGTCGGA GCTGCTGGGT CCATATGGGA TGAAGTTCCT CAGCGAGAGT
    CTCATGTGGC ACATCTCATC CCAAGTTGCA GAACTGAAGA AACTTGTGGT GGACAATGTG
    GAGGTTTTAA CCCAGATGAG GACCAGCTTT GACAAGCCGG ACCATATGGC AGCACTCTTT
    AAGAGGCTAA CATCGGTTGA CAGCGTGCTG AAACGGATGA CCATCATTGG TGTGATTTTG
    TCGTTCCGCT CTCTGGCTCA GGAAGCTCTC CGAGATGTGC TCTCTTGCCA CATTCCCTTC
    CTGGTGAGCT CCGTTGAAGA CTTTAAAGAT CACATTCCTC GTGAAACTGA CATGAAGGTG
    GCCATGAACG TGTACGAGCT GTCTTCTGCT GCAGGCCTGC CCTGTGAGAT CGACCCTGCT
    CTAGTAGTGG CGCTGTCATC CCAGAAGTCT GAAAATATTA GCCCGGAGGA AGAGTACAAG
    ATCGCCTGCC TGTTGATGGT CTTCGTGGCC GTTTCCATGC CGACCCTGGC CAGCAACGTG
    ATGTCACAGT ACAGTCCCGC AATAGAAGGT CACTGCAACA ACATTCACTG TCTGGCTAAA
    GCGATAAACC AGATTGCCGC CGCTCTGTTC ACGATCCACA AGGGCAGCAT AGAGGATCGC
    CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC AGCCTGCTGA AGATTGGACA GGAGACGGAT
    AAAACCACTA CACGGAACAG AGAGTCTGTT TACCTGCTCC TGGACATGAT CGTTCAAGAA
    TCGCCATTCC TCACCATGGA CCTGTTGGAG TCCTGCTTTC CATACGTCCT GCTCCGAAAC
    GCCTATCACG CCGTATACAA GCAGTCGGTA TCTTCTTCAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15520
    Clone ID Related Accession (Same CDS sequence) XM_005167921.4 , XM_005167921.3
    Accession Version XM_005167921.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nck-associated protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT
    TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG
    TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCAA
    CAGCTTGCAC AACTACAAAA AGAAAAGTCA GAAATACTGA AGAATTTAGC TCTATATTAC
    TTCACCTTTG TGGACGTTAT GGAATTTAAG GACCACGTTT GTGAACTGCT GAACACTATT
    GATGCTTGTC AAGTTTTCTT TGACATTACT GTAAATTTTG ACCTCACAAA GAACTACCTG
    GACTTGGTAG TGACGTACAC TACCCTGATG TTGCTGCTTT CCCGAATTGA AGAGCGCAAA
    GCGATAATCG GCCTCTACAA CTATGCCCAT GAAATGACAC ACGGTTCAAG CGATCGAGAA
    TACCCCAGGC TTGGACAGAT GATTGTGGAT TACGAGAACC CACTAAAAAA GATGATGGAG
    GAGTTTGTCC CTCATGGAAA ATCGCTGTCA GATGCGCTGG TCTCCTTGCA GATGGTGTAC
    CCACGCCGGA ATCTGTCTGC AGATCAGTGG AGAAACGCTC AGCTGCTCAG TTTGATTAGC
    GCTCCCAGCA CTATGCTCAA CCCAGCTCAA TCTGACACGA TGCCCTGTGA ATACCTATCC
    TTGGACACCA TGGAGAAATG GATCGTCTTC GGCTTCATCT TGTGTCATGC GGCTCTGAAC
    AGTGATCCTG CGGCTCTCAG TCTTTGGAAG TTGGCCCTTC AGAGCAGTAC CTGTCTCTGT
    CTGTTCAGGG ACGAGGTCTT CCACATTCAC AAAGCTGCAG AGGATCTCTT CGTAAACATT
    CGCGGTTACA ACAAACGTGT CAATGATATT CGTGAATGCA AAGAATCTGC TCTTTCACAT
    GCGGGCTCAA TGCACAGAGA ACGACGTAAG TTCTTGAGGT CTGCCCTGAA AGAGCTAGCC
    ACAGTACTGG CAGATCAGCC TGGATTGCTG GGTCCGAAGG CTCTGTTTGT GTTCATGGCA
    CTCTCCTTCG CCCGTGATGA GATCATTTGG CTGCTCAGAC ATGCCGACAA CATCCAAAAG
    AAAAGCACAG ATGACTTCAT TGACAAGCAC ATTGCAGAGT TGATCTTCTA CATGGAGGAG
    TTGAGGGCTC ACGTCCGAAA GTACGGGCCG GTGATGCAGA GGTACTATGT TCAGTACCTG
    TCCGGCTTTG ACGCGGTGGT GCTCAATGAG CTTGTACAGA ACCTGTCCGT GTGCCCAGAG
    GACGAGTCCA TCATAATGTC GTCCTTCGTG AACACTATGA CCTCTCTCAG TGTTAAACAA
    GTTGAAGATG GCGAAGTGTT TGATTTCAGA GGGATGAGGC TGGACTGGTT CAGACTTCAG
    GCCTACACCA GCGTGTCCAA GGCTTCGTTG GGACTGGCAG ATCACAGAGA ACTGGGAAAG
    ATGATGAACA CCATCATCTT CCACACTAAG ATGGTGGACT CTCTGGTGGA GATGCTTGTG
    GAGACTTCTG ACCTGTCCAT ATTCTGTTTT TACAGCCGCG CCTTTGAAAA GATGTTCCAG
    CAGTGTCTTG AACTGCCGTC CCAGTCTCGA TACTCCATCT CCTTCCCACT GCTCTGCACC
    CACTTTATGA GCTGCACTCA TGAGCTCTGC CCTGAAGAGA GGCACCACAT CGGAGACCGC
    AGCCTGTCCC TTTGCAACAT GTTCCTGGAC GAGATGGCCA AGCAAGCCCG CAACCTCATC
    ACAGACATAT GCACAGAGCA GTGCACCCTG AGCGACCAGC TACTGCCAAA ACATTGTGCC
    AAAACCATCA GTCAGGCCGT GAACAAGAAG TCGAAAAAGC AGACAGGGAA GAAGGGTGAA
    CCGGAGCGAG AAAAACCTGG CGTGGAGAGC ATGAGGAAGA ACAGACTCCT GGTCACCAAT
    CTGGATAAAC TGCACACTGC TCTGTCCGAG CTCTGCTTCT CCATCAACTA CGTCCCCAAC
    ATGATGGTCT GGGAGCACAC CTTCACCCCA CGCGAGTACC TGACCTCCCA CCTGGAGATT
    CGCTTCACTA AGTCTATAGT CGGGATGACC ATGTACAACC AGACAACACA GGAGATCGCC
    AAACCATCAG AGCTGCTCAC CAGCGTCCGG GCCTACATGA CAGTGCTGCA GTCCATTGAG
    AATTATGTGC AGATTGACAT CACCAGAGTC TTCAACAATG TGCTGCTGCA GCAAACCCAA
    CACCTGGACA GCCACGGAGA GCCAACCATC ACCAGCCTCT ACACTAACTG GTATCTGGAG
    ACTTTACTGC GGCAGGTCAG CAACGGTCAC ATCGCGTACT TTCCTGCGAT GAAAGCCTTC
    GTCAACTTGC CCACGGAGAA TGAGCTGACC TTCAACGCAG AGGAGTATTC TGACATTTCT
    GAAATGCGCT CTCTGTCGGA GCTGCTGGGT CCATATGGGA TGAAGTTCCT CAGCGAGAGT
    CTCATGTGGC ACATCTCATC CCAAGTTGCA GAACTGAAGA AACTTGTGGT GGACAATGTG
    GAGGTTTTAA CCCAGATGAG GACCAGCTTT GACAAGCCGG ACCATATGGC AGCACTCTTT
    AAGAGGCTAA CATCGGTTGA CAGCGTGCTG AAACGGATGA CCATCATTGG TGTGATTTTG
    TCGTTCCGCT CTCTGGCTCA GGAAGCTCTC CGAGATGTGC TCTCTTGCCA CATTCCCTTC
    CTGGTGAGCT CCGTTGAAGA CTTTAAAGAT CACATTCCTC GTGAAACTGA CATGAAGGTG
    GCCATGAACG TGTACGAGCT GTCTTCTGCT GCAGGCCTGC CCTGTGAGAT CGACCCTGCT
    CTAGTAGTGG CGCTGTCATC CCAGAAGTCT GAAAATATTA GCCCGGAGGA AGAGTACAAG
    ATCGCCTGCC TGTTGATGGT CTTCGTGGCC GTTTCCATGC CGACCCTGGC CAGCAACGTG
    ATGTCACAGT ACAGTCCCGC AATAGAAGGT CACTGCAACA ACATTCACTG TCTGGCTAAA
    GCGATAAACC AGATTGCCGC CGCTCTGTTC ACGATCCACA AGGGCAGCAT AGAGGATCGC
    CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC AGCCTGCTGA AGATTGGACA GGAGACGGAT
    AAAACCACTA CACGGAACAG AGAGTCTGTT TACCTGCTCC TGGACATGAT CGTTCAAGAA
    TCGCCATTCC TCACCATGGA CCTGTTGGAG TCCTGCTTTC CATACGTCCT GCTCCGAAAC
    GCCTATCACG CCGTATACAA GCAGTCGGTA TCTTCTTCAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167978.1
    CDS1..3405
    Translation

    Target ORF information:

    RefSeq Version XM_005167921.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167921.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT
    TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG
    TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCAA
    CAGCTTGCAC AACTACAAAA AGAAAAGTCA GAAATACTGA AGAATTTAGC TCTATATTAC
    TTCACCTTTG TGGACGTTAT GGAATTTAAG GACCACGTTT GTGAACTGCT GAACACTATT
    GATGCTTGTC AAGTTTTCTT TGACATTACT GTAAATTTTG ACCTCACAAA GAACTACCTG
    GACTTGGTAG TGACGTACAC TACCCTGATG TTGCTGCTTT CCCGAATTGA AGAGCGCAAA
    GCGATAATCG GCCTCTACAA CTATGCCCAT GAAATGACAC ACGGTTCAAG CGATCGAGAA
    TACCCCAGGC TTGGACAGAT GATTGTGGAT TACGAGAACC CACTAAAAAA GATGATGGAG
    GAGTTTGTCC CTCATGGAAA ATCGCTGTCA GATGCGCTGG TCTCCTTGCA GATGGTGTAC
    CCACGCCGGA ATCTGTCTGC AGATCAGTGG AGAAACGCTC AGCTGCTCAG TTTGATTAGC
    GCTCCCAGCA CTATGCTCAA CCCAGCTCAA TCTGACACGA TGCCCTGTGA ATACCTATCC
    TTGGACACCA TGGAGAAATG GATCGTCTTC GGCTTCATCT TGTGTCATGC GGCTCTGAAC
    AGTGATCCTG CGGCTCTCAG TCTTTGGAAG TTGGCCCTTC AGAGCAGTAC CTGTCTCTGT
    CTGTTCAGGG ACGAGGTCTT CCACATTCAC AAAGCTGCAG AGGATCTCTT CGTAAACATT
    CGCGGTTACA ACAAACGTGT CAATGATATT CGTGAATGCA AAGAATCTGC TCTTTCACAT
    GCGGGCTCAA TGCACAGAGA ACGACGTAAG TTCTTGAGGT CTGCCCTGAA AGAGCTAGCC
    ACAGTACTGG CAGATCAGCC TGGATTGCTG GGTCCGAAGG CTCTGTTTGT GTTCATGGCA
    CTCTCCTTCG CCCGTGATGA GATCATTTGG CTGCTCAGAC ATGCCGACAA CATCCAAAAG
    AAAAGCACAG ATGACTTCAT TGACAAGCAC ATTGCAGAGT TGATCTTCTA CATGGAGGAG
    TTGAGGGCTC ACGTCCGAAA GTACGGGCCG GTGATGCAGA GGTACTATGT TCAGTACCTG
    TCCGGCTTTG ACGCGGTGGT GCTCAATGAG CTTGTACAGA ACCTGTCCGT GTGCCCAGAG
    GACGAGTCCA TCATAATGTC GTCCTTCGTG AACACTATGA CCTCTCTCAG TGTTAAACAA
    GTTGAAGATG GCGAAGTGTT TGATTTCAGA GGGATGAGGC TGGACTGGTT CAGACTTCAG
    GCCTACACCA GCGTGTCCAA GGCTTCGTTG GGACTGGCAG ATCACAGAGA ACTGGGAAAG
    ATGATGAACA CCATCATCTT CCACACTAAG ATGGTGGACT CTCTGGTGGA GATGCTTGTG
    GAGACTTCTG ACCTGTCCAT ATTCTGTTTT TACAGCCGCG CCTTTGAAAA GATGTTCCAG
    CAGTGTCTTG AACTGCCGTC CCAGTCTCGA TACTCCATCT CCTTCCCACT GCTCTGCACC
    CACTTTATGA GCTGCACTCA TGAGCTCTGC CCTGAAGAGA GGCACCACAT CGGAGACCGC
    AGCCTGTCCC TTTGCAACAT GTTCCTGGAC GAGATGGCCA AGCAAGCCCG CAACCTCATC
    ACAGACATAT GCACAGAGCA GTGCACCCTG AGCGACCAGC TACTGCCAAA ACATTGTGCC
    AAAACCATCA GTCAGGCCGT GAACAAGAAG TCGAAAAAGC AGACAGGGAA GAAGGGTGAA
    CCGGAGCGAG AAAAACCTGG CGTGGAGAGC ATGAGGAAGA ACAGACTCCT GGTCACCAAT
    CTGGATAAAC TGCACACTGC TCTGTCCGAG CTCTGCTTCT CCATCAACTA CGTCCCCAAC
    ATGATGGTCT GGGAGCACAC CTTCACCCCA CGCGAGTACC TGACCTCCCA CCTGGAGATT
    CGCTTCACTA AGTCTATAGT CGGGATGACC ATGTACAACC AGACAACACA GGAGATCGCC
    AAACCATCAG AGCTGCTCAC CAGCGTCCGG GCCTACATGA CAGTGCTGCA GTCCATTGAG
    AATTATGTGC AGATTGACAT CACCAGAGTC TTCAACAATG TGCTGCTGCA GCAAACCCAA
    CACCTGGACA GCCACGGAGA GCCAACCATC ACCAGCCTCT ACACTAACTG GTATCTGGAG
    ACTTTACTGC GGCAGGTCAG CAACGGTCAC ATCGCGTACT TTCCTGCGAT GAAAGCCTTC
    GTCAACTTGC CCACGGAGAA TGAGCTGACC TTCAACGCAG AGGAGTATTC TGACATTTCT
    GAAATGCGCT CTCTGTCGGA GCTGCTGGGT CCATATGGGA TGAAGTTCCT CAGCGAGAGT
    CTCATGTGGC ACATCTCATC CCAAGTTGCA GAACTGAAGA AACTTGTGGT GGACAATGTG
    GAGGTTTTAA CCCAGATGAG GACCAGCTTT GACAAGCCGG ACCATATGGC AGCACTCTTT
    AAGAGGCTAA CATCGGTTGA CAGCGTGCTG AAACGGATGA CCATCATTGG TGTGATTTTG
    TCGTTCCGCT CTCTGGCTCA GGAAGCTCTC CGAGATGTGC TCTCTTGCCA CATTCCCTTC
    CTGGTGAGCT CCGTTGAAGA CTTTAAAGAT CACATTCCTC GTGAAACTGA CATGAAGGTG
    GCCATGAACG TGTACGAGCT GTCTTCTGCT GCAGGCCTGC CCTGTGAGAT CGACCCTGCT
    CTAGTAGTGG CGCTGTCATC CCAGAAGTCT GAAAATATTA GCCCGGAGGA AGAGTACAAG
    ATCGCCTGCC TGTTGATGGT CTTCGTGGCC GTTTCCATGC CGACCCTGGC CAGCAACGTG
    ATGTCACAGT ACAGTCCCGC AATAGAAGGT CACTGCAACA ACATTCACTG TCTGGCTAAA
    GCGATAAACC AGATTGCCGC CGCTCTGTTC ACGATCCACA AGGGCAGCAT AGAGGATCGC
    CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC AGCCTGCTGA AGATTGGACA GGAGACGGAT
    AAAACCACTA CACGGAACAG AGAGTCTGTT TACCTGCTCC TGGACATGAT CGTTCAAGAA
    TCGCCATTCC TCACCATGGA CCTGTTGGAG TCCTGCTTTC CATACGTCCT GCTCCGAAAC
    GCCTATCACG CCGTATACAA GCAGTCGGTA TCTTCTTCAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15521
    Clone ID Related Accession (Same CDS sequence) XM_005167922.4 , XM_005167922.3
    Accession Version XM_005167922.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167922.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT
    TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG
    TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCTT
    GCACAACTAC AAAAAGAAAA GTCAGAAATA CTGAAGAATT TAGCTCTATA TTACTTCACC
    TTTGTGGACG TTATGGAATT TAAGGACCAC GTTTGTGAAC TGCTGAACAC TATTGATGCT
    TGTCAAGTTT TCTTTGACAT TACTGTAAAT TTTGACCTCA CAAAGAACTA CCTGGACTTG
    GTAGTGACGT ACACTACCCT GATGTTGCTG CTTTCCCGAA TTGAAGAGCG CAAAGCGATA
    ATCGGCCTCT ACAACTATGC CCATGAAATG ACACACGGTT CAAGCGATCG AGAATACCCC
    AGGCTTGGAC AGATGATTGT GGATTACGAG AACCCACTAA AAAAGATGAT GGAGGAGTTT
    GTCCCTCATG GAAAATCGCT GTCAGATGCG CTGGTCTCCT TGCAGATGGT GTACCCACGC
    CGGAATCTGT CTGCAGATCA GTGGAGAAAC GCTCAGCTGC TCAGTTTGAT TAGCGCTCCC
    AGCACTATGC TCAACCCAGC TCAATCTGAC ACGATGCCCT GTGAATACCT ATCCTTGGAC
    ACCATGGAGA AATGGATCGT CTTCGGCTTC ATCTTGTGTC ATGCGGCTCT GAACAGTGAT
    CCTGCGGCTC TCAGTCTTTG GAAGTTGGCC CTTCAGAGCA GTACCTGTCT CTGTCTGTTC
    AGGGACGAGG TCTTCCACAT TCACAAAGCT GCAGAGGATC TCTTCGTAAA CATTCGCGGT
    TACAACAAAC GTGTCAATGA TATTCGTGAA TGCAAAGAAT CTGCTCTTTC ACATGCGGGC
    TCAATGCACA GAGAACGACG TAAGTTCTTG AGGTCTGCCC TGAAAGAGCT AGCCACAGTA
    CTGGCAGATC AGCCTGGATT GCTGGGTCCG AAGGCTCTGT TTGTGTTCAT GGCACTCTCC
    TTCGCCCGTG ATGAGATCAT TTGGCTGCTC AGACATGCCG ACAACATCCA AAAGAAAAGC
    ACAGATGACT TCATTGACAA GCACATTGCA GAGTTGATCT TCTACATGGA GGAGTTGAGG
    GCTCACGTCC GAAAGTACGG GCCGGTGATG CAGAGGTACT ATGTTCAGTA CCTGTCCGGC
    TTTGACGCGG TGGTGCTCAA TGAGCTTGTA CAGAACCTGT CCGTGTGCCC AGAGGACGAG
    TCCATCATAA TGTCGTCCTT CGTGAACACT ATGACCTCTC TCAGTGTTAA ACAAGTTGAA
    GATGGCGAAG TGTTTGATTT CAGAGGGATG AGGCTGGACT GGTTCAGACT TCAGGCCTAC
    ACCAGCGTGT CCAAGGCTTC GTTGGGACTG GCAGATCACA GAGAACTGGG AAAGATGATG
    AACACCATCA TCTTCCACAC TAAGATGGTG GACTCTCTGG TGGAGATGCT TGTGGAGACT
    TCTGACCTGT CCATATTCTG TTTTTACAGC CGCGCCTTTG AAAAGATGTT CCAGCAGTGT
    CTTGAACTGC CGTCCCAGTC TCGATACTCC ATCTCCTTCC CACTGCTCTG CACCCACTTT
    ATGAGCTGCA CTCATGAGCT CTGCCCTGAA GAGAGGCACC ACATCGGAGA CCGCAGCCTG
    TCCCTTTGCA ACATGTTCCT GGACGAGATG GCCAAGCAAG CCCGCAACCT CATCACAGAC
    ATATGCACAG AGCAGTGCAC CCTGAGCGAC CAGCTACTGC CAAAACATTG TGCCAAAACC
    ATCAGTCAGG CCGTGAACAA GAAGTCGAAA AAGCAGACAG GGAAGAAGGG TGAACCGGAG
    CGAGAAAAAC CTGGCGTGGA GAGCATGAGG AAGAACAGAC TCCTGGTCAC CAATCTGGAT
    AAACTGCACA CTGCTCTGTC CGAGCTCTGC TTCTCCATCA ACTACGTCCC CAACATGATG
    GTCTGGGAGC ACACCTTCAC CCCACGCGAG TACCTGACCT CCCACCTGGA GATTCGCTTC
    ACTAAGTCTA TAGTCGGGAT GACCATGTAC AACCAGACAA CACAGGAGAT CGCCAAACCA
    TCAGAGCTGC TCACCAGCGT CCGGGCCTAC ATGACAGTGC TGCAGTCCAT TGAGAATTAT
    GTGCAGATTG ACATCACCAG AGTCTTCAAC AATGTGCTGC TGCAGCAAAC CCAACACCTG
    GACAGCCACG GAGAGCCAAC CATCACCAGC CTCTACACTA ACTGGTATCT GGAGACTTTA
    CTGCGGCAGG TCAGCAACGG TCACATCGCG TACTTTCCTG CGATGAAAGC CTTCGTCAAC
    TTGCCCACGG AGAATGAGCT GACCTTCAAC GCAGAGGAGT ATTCTGACAT TTCTGAAATG
    CGCTCTCTGT CGGAGCTGCT GGGTCCATAT GGGATGAAGT TCCTCAGCGA GAGTCTCATG
    TGGCACATCT CATCCCAAGT TGCAGAACTG AAGAAACTTG TGGTGGACAA TGTGGAGGTT
    TTAACCCAGA TGAGGACCAG CTTTGACAAG CCGGACCATA TGGCAGCACT CTTTAAGAGG
    CTAACATCGG TTGACAGCGT GCTGAAACGG ATGACCATCA TTGGTGTGAT TTTGTCGTTC
    CGCTCTCTGG CTCAGGAAGC TCTCCGAGAT GTGCTCTCTT GCCACATTCC CTTCCTGGTG
    AGCTCCGTTG AAGACTTTAA AGATCACATT CCTCGTGAAA CTGACATGAA GGTGGCCATG
    AACGTGTACG AGCTGTCTTC TGCTGCAGGC CTGCCCTGTG AGATCGACCC TGCTCTAGTA
    GTGGCGCTGT CATCCCAGAA GTCTGAAAAT ATTAGCCCGG AGGAAGAGTA CAAGATCGCC
    TGCCTGTTGA TGGTCTTCGT GGCCGTTTCC ATGCCGACCC TGGCCAGCAA CGTGATGTCA
    CAGTACAGTC CCGCAATAGA AGGTCACTGC AACAACATTC ACTGTCTGGC TAAAGCGATA
    AACCAGATTG CCGCCGCTCT GTTCACGATC CACAAGGGCA GCATAGAGGA TCGCCTCAAA
    GAATTCCTCG CCCTCGCCTC CTCCAGCCTG CTGAAGATTG GACAGGAGAC GGATAAAACC
    ACTACACGGA ACAGAGAGTC TGTTTACCTG CTCCTGGACA TGATCGTTCA AGAATCGCCA
    TTCCTCACCA TGGACCTGTT GGAGTCCTGC TTTCCATACG TCCTGCTCCG AAACGCCTAT
    CACGCCGTAT ACAAGCAGTC GGTATCTTCT TCAGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167979.1
    CDS212..3610
    Translation

    Target ORF information:

    RefSeq Version XM_005167922.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167922.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT
    TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG
    TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCTT
    GCACAACTAC AAAAAGAAAA GTCAGAAATA CTGAAGAATT TAGCTCTATA TTACTTCACC
    TTTGTGGACG TTATGGAATT TAAGGACCAC GTTTGTGAAC TGCTGAACAC TATTGATGCT
    TGTCAAGTTT TCTTTGACAT TACTGTAAAT TTTGACCTCA CAAAGAACTA CCTGGACTTG
    GTAGTGACGT ACACTACCCT GATGTTGCTG CTTTCCCGAA TTGAAGAGCG CAAAGCGATA
    ATCGGCCTCT ACAACTATGC CCATGAAATG ACACACGGTT CAAGCGATCG AGAATACCCC
    AGGCTTGGAC AGATGATTGT GGATTACGAG AACCCACTAA AAAAGATGAT GGAGGAGTTT
    GTCCCTCATG GAAAATCGCT GTCAGATGCG CTGGTCTCCT TGCAGATGGT GTACCCACGC
    CGGAATCTGT CTGCAGATCA GTGGAGAAAC GCTCAGCTGC TCAGTTTGAT TAGCGCTCCC
    AGCACTATGC TCAACCCAGC TCAATCTGAC ACGATGCCCT GTGAATACCT ATCCTTGGAC
    ACCATGGAGA AATGGATCGT CTTCGGCTTC ATCTTGTGTC ATGCGGCTCT GAACAGTGAT
    CCTGCGGCTC TCAGTCTTTG GAAGTTGGCC CTTCAGAGCA GTACCTGTCT CTGTCTGTTC
    AGGGACGAGG TCTTCCACAT TCACAAAGCT GCAGAGGATC TCTTCGTAAA CATTCGCGGT
    TACAACAAAC GTGTCAATGA TATTCGTGAA TGCAAAGAAT CTGCTCTTTC ACATGCGGGC
    TCAATGCACA GAGAACGACG TAAGTTCTTG AGGTCTGCCC TGAAAGAGCT AGCCACAGTA
    CTGGCAGATC AGCCTGGATT GCTGGGTCCG AAGGCTCTGT TTGTGTTCAT GGCACTCTCC
    TTCGCCCGTG ATGAGATCAT TTGGCTGCTC AGACATGCCG ACAACATCCA AAAGAAAAGC
    ACAGATGACT TCATTGACAA GCACATTGCA GAGTTGATCT TCTACATGGA GGAGTTGAGG
    GCTCACGTCC GAAAGTACGG GCCGGTGATG CAGAGGTACT ATGTTCAGTA CCTGTCCGGC
    TTTGACGCGG TGGTGCTCAA TGAGCTTGTA CAGAACCTGT CCGTGTGCCC AGAGGACGAG
    TCCATCATAA TGTCGTCCTT CGTGAACACT ATGACCTCTC TCAGTGTTAA ACAAGTTGAA
    GATGGCGAAG TGTTTGATTT CAGAGGGATG AGGCTGGACT GGTTCAGACT TCAGGCCTAC
    ACCAGCGTGT CCAAGGCTTC GTTGGGACTG GCAGATCACA GAGAACTGGG AAAGATGATG
    AACACCATCA TCTTCCACAC TAAGATGGTG GACTCTCTGG TGGAGATGCT TGTGGAGACT
    TCTGACCTGT CCATATTCTG TTTTTACAGC CGCGCCTTTG AAAAGATGTT CCAGCAGTGT
    CTTGAACTGC CGTCCCAGTC TCGATACTCC ATCTCCTTCC CACTGCTCTG CACCCACTTT
    ATGAGCTGCA CTCATGAGCT CTGCCCTGAA GAGAGGCACC ACATCGGAGA CCGCAGCCTG
    TCCCTTTGCA ACATGTTCCT GGACGAGATG GCCAAGCAAG CCCGCAACCT CATCACAGAC
    ATATGCACAG AGCAGTGCAC CCTGAGCGAC CAGCTACTGC CAAAACATTG TGCCAAAACC
    ATCAGTCAGG CCGTGAACAA GAAGTCGAAA AAGCAGACAG GGAAGAAGGG TGAACCGGAG
    CGAGAAAAAC CTGGCGTGGA GAGCATGAGG AAGAACAGAC TCCTGGTCAC CAATCTGGAT
    AAACTGCACA CTGCTCTGTC CGAGCTCTGC TTCTCCATCA ACTACGTCCC CAACATGATG
    GTCTGGGAGC ACACCTTCAC CCCACGCGAG TACCTGACCT CCCACCTGGA GATTCGCTTC
    ACTAAGTCTA TAGTCGGGAT GACCATGTAC AACCAGACAA CACAGGAGAT CGCCAAACCA
    TCAGAGCTGC TCACCAGCGT CCGGGCCTAC ATGACAGTGC TGCAGTCCAT TGAGAATTAT
    GTGCAGATTG ACATCACCAG AGTCTTCAAC AATGTGCTGC TGCAGCAAAC CCAACACCTG
    GACAGCCACG GAGAGCCAAC CATCACCAGC CTCTACACTA ACTGGTATCT GGAGACTTTA
    CTGCGGCAGG TCAGCAACGG TCACATCGCG TACTTTCCTG CGATGAAAGC CTTCGTCAAC
    TTGCCCACGG AGAATGAGCT GACCTTCAAC GCAGAGGAGT ATTCTGACAT TTCTGAAATG
    CGCTCTCTGT CGGAGCTGCT GGGTCCATAT GGGATGAAGT TCCTCAGCGA GAGTCTCATG
    TGGCACATCT CATCCCAAGT TGCAGAACTG AAGAAACTTG TGGTGGACAA TGTGGAGGTT
    TTAACCCAGA TGAGGACCAG CTTTGACAAG CCGGACCATA TGGCAGCACT CTTTAAGAGG
    CTAACATCGG TTGACAGCGT GCTGAAACGG ATGACCATCA TTGGTGTGAT TTTGTCGTTC
    CGCTCTCTGG CTCAGGAAGC TCTCCGAGAT GTGCTCTCTT GCCACATTCC CTTCCTGGTG
    AGCTCCGTTG AAGACTTTAA AGATCACATT CCTCGTGAAA CTGACATGAA GGTGGCCATG
    AACGTGTACG AGCTGTCTTC TGCTGCAGGC CTGCCCTGTG AGATCGACCC TGCTCTAGTA
    GTGGCGCTGT CATCCCAGAA GTCTGAAAAT ATTAGCCCGG AGGAAGAGTA CAAGATCGCC
    TGCCTGTTGA TGGTCTTCGT GGCCGTTTCC ATGCCGACCC TGGCCAGCAA CGTGATGTCA
    CAGTACAGTC CCGCAATAGA AGGTCACTGC AACAACATTC ACTGTCTGGC TAAAGCGATA
    AACCAGATTG CCGCCGCTCT GTTCACGATC CACAAGGGCA GCATAGAGGA TCGCCTCAAA
    GAATTCCTCG CCCTCGCCTC CTCCAGCCTG CTGAAGATTG GACAGGAGAC GGATAAAACC
    ACTACACGGA ACAGAGAGTC TGTTTACCTG CTCCTGGACA TGATCGTTCA AGAATCGCCA
    TTCCTCACCA TGGACCTGTT GGAGTCCTGC TTTCCATACG TCCTGCTCCG AAACGCCTAT
    CACGCCGTAT ACAAGCAGTC GGTATCTTCT TCAGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15521
    Clone ID Related Accession (Same CDS sequence) XM_005167922.4 , XM_005167922.3
    Accession Version XM_005167922.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nck-associated protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT
    TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG
    TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCTT
    GCACAACTAC AAAAAGAAAA GTCAGAAATA CTGAAGAATT TAGCTCTATA TTACTTCACC
    TTTGTGGACG TTATGGAATT TAAGGACCAC GTTTGTGAAC TGCTGAACAC TATTGATGCT
    TGTCAAGTTT TCTTTGACAT TACTGTAAAT TTTGACCTCA CAAAGAACTA CCTGGACTTG
    GTAGTGACGT ACACTACCCT GATGTTGCTG CTTTCCCGAA TTGAAGAGCG CAAAGCGATA
    ATCGGCCTCT ACAACTATGC CCATGAAATG ACACACGGTT CAAGCGATCG AGAATACCCC
    AGGCTTGGAC AGATGATTGT GGATTACGAG AACCCACTAA AAAAGATGAT GGAGGAGTTT
    GTCCCTCATG GAAAATCGCT GTCAGATGCG CTGGTCTCCT TGCAGATGGT GTACCCACGC
    CGGAATCTGT CTGCAGATCA GTGGAGAAAC GCTCAGCTGC TCAGTTTGAT TAGCGCTCCC
    AGCACTATGC TCAACCCAGC TCAATCTGAC ACGATGCCCT GTGAATACCT ATCCTTGGAC
    ACCATGGAGA AATGGATCGT CTTCGGCTTC ATCTTGTGTC ATGCGGCTCT GAACAGTGAT
    CCTGCGGCTC TCAGTCTTTG GAAGTTGGCC CTTCAGAGCA GTACCTGTCT CTGTCTGTTC
    AGGGACGAGG TCTTCCACAT TCACAAAGCT GCAGAGGATC TCTTCGTAAA CATTCGCGGT
    TACAACAAAC GTGTCAATGA TATTCGTGAA TGCAAAGAAT CTGCTCTTTC ACATGCGGGC
    TCAATGCACA GAGAACGACG TAAGTTCTTG AGGTCTGCCC TGAAAGAGCT AGCCACAGTA
    CTGGCAGATC AGCCTGGATT GCTGGGTCCG AAGGCTCTGT TTGTGTTCAT GGCACTCTCC
    TTCGCCCGTG ATGAGATCAT TTGGCTGCTC AGACATGCCG ACAACATCCA AAAGAAAAGC
    ACAGATGACT TCATTGACAA GCACATTGCA GAGTTGATCT TCTACATGGA GGAGTTGAGG
    GCTCACGTCC GAAAGTACGG GCCGGTGATG CAGAGGTACT ATGTTCAGTA CCTGTCCGGC
    TTTGACGCGG TGGTGCTCAA TGAGCTTGTA CAGAACCTGT CCGTGTGCCC AGAGGACGAG
    TCCATCATAA TGTCGTCCTT CGTGAACACT ATGACCTCTC TCAGTGTTAA ACAAGTTGAA
    GATGGCGAAG TGTTTGATTT CAGAGGGATG AGGCTGGACT GGTTCAGACT TCAGGCCTAC
    ACCAGCGTGT CCAAGGCTTC GTTGGGACTG GCAGATCACA GAGAACTGGG AAAGATGATG
    AACACCATCA TCTTCCACAC TAAGATGGTG GACTCTCTGG TGGAGATGCT TGTGGAGACT
    TCTGACCTGT CCATATTCTG TTTTTACAGC CGCGCCTTTG AAAAGATGTT CCAGCAGTGT
    CTTGAACTGC CGTCCCAGTC TCGATACTCC ATCTCCTTCC CACTGCTCTG CACCCACTTT
    ATGAGCTGCA CTCATGAGCT CTGCCCTGAA GAGAGGCACC ACATCGGAGA CCGCAGCCTG
    TCCCTTTGCA ACATGTTCCT GGACGAGATG GCCAAGCAAG CCCGCAACCT CATCACAGAC
    ATATGCACAG AGCAGTGCAC CCTGAGCGAC CAGCTACTGC CAAAACATTG TGCCAAAACC
    ATCAGTCAGG CCGTGAACAA GAAGTCGAAA AAGCAGACAG GGAAGAAGGG TGAACCGGAG
    CGAGAAAAAC CTGGCGTGGA GAGCATGAGG AAGAACAGAC TCCTGGTCAC CAATCTGGAT
    AAACTGCACA CTGCTCTGTC CGAGCTCTGC TTCTCCATCA ACTACGTCCC CAACATGATG
    GTCTGGGAGC ACACCTTCAC CCCACGCGAG TACCTGACCT CCCACCTGGA GATTCGCTTC
    ACTAAGTCTA TAGTCGGGAT GACCATGTAC AACCAGACAA CACAGGAGAT CGCCAAACCA
    TCAGAGCTGC TCACCAGCGT CCGGGCCTAC ATGACAGTGC TGCAGTCCAT TGAGAATTAT
    GTGCAGATTG ACATCACCAG AGTCTTCAAC AATGTGCTGC TGCAGCAAAC CCAACACCTG
    GACAGCCACG GAGAGCCAAC CATCACCAGC CTCTACACTA ACTGGTATCT GGAGACTTTA
    CTGCGGCAGG TCAGCAACGG TCACATCGCG TACTTTCCTG CGATGAAAGC CTTCGTCAAC
    TTGCCCACGG AGAATGAGCT GACCTTCAAC GCAGAGGAGT ATTCTGACAT TTCTGAAATG
    CGCTCTCTGT CGGAGCTGCT GGGTCCATAT GGGATGAAGT TCCTCAGCGA GAGTCTCATG
    TGGCACATCT CATCCCAAGT TGCAGAACTG AAGAAACTTG TGGTGGACAA TGTGGAGGTT
    TTAACCCAGA TGAGGACCAG CTTTGACAAG CCGGACCATA TGGCAGCACT CTTTAAGAGG
    CTAACATCGG TTGACAGCGT GCTGAAACGG ATGACCATCA TTGGTGTGAT TTTGTCGTTC
    CGCTCTCTGG CTCAGGAAGC TCTCCGAGAT GTGCTCTCTT GCCACATTCC CTTCCTGGTG
    AGCTCCGTTG AAGACTTTAA AGATCACATT CCTCGTGAAA CTGACATGAA GGTGGCCATG
    AACGTGTACG AGCTGTCTTC TGCTGCAGGC CTGCCCTGTG AGATCGACCC TGCTCTAGTA
    GTGGCGCTGT CATCCCAGAA GTCTGAAAAT ATTAGCCCGG AGGAAGAGTA CAAGATCGCC
    TGCCTGTTGA TGGTCTTCGT GGCCGTTTCC ATGCCGACCC TGGCCAGCAA CGTGATGTCA
    CAGTACAGTC CCGCAATAGA AGGTCACTGC AACAACATTC ACTGTCTGGC TAAAGCGATA
    AACCAGATTG CCGCCGCTCT GTTCACGATC CACAAGGGCA GCATAGAGGA TCGCCTCAAA
    GAATTCCTCG CCCTCGCCTC CTCCAGCCTG CTGAAGATTG GACAGGAGAC GGATAAAACC
    ACTACACGGA ACAGAGAGTC TGTTTACCTG CTCCTGGACA TGATCGTTCA AGAATCGCCA
    TTCCTCACCA TGGACCTGTT GGAGTCCTGC TTTCCATACG TCCTGCTCCG AAACGCCTAT
    CACGCCGTAT ACAAGCAGTC GGTATCTTCT TCAGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167979.1
    CDS230..3628
    Translation

    Target ORF information:

    RefSeq Version XM_005167922.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167922.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT
    TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG
    TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCTT
    GCACAACTAC AAAAAGAAAA GTCAGAAATA CTGAAGAATT TAGCTCTATA TTACTTCACC
    TTTGTGGACG TTATGGAATT TAAGGACCAC GTTTGTGAAC TGCTGAACAC TATTGATGCT
    TGTCAAGTTT TCTTTGACAT TACTGTAAAT TTTGACCTCA CAAAGAACTA CCTGGACTTG
    GTAGTGACGT ACACTACCCT GATGTTGCTG CTTTCCCGAA TTGAAGAGCG CAAAGCGATA
    ATCGGCCTCT ACAACTATGC CCATGAAATG ACACACGGTT CAAGCGATCG AGAATACCCC
    AGGCTTGGAC AGATGATTGT GGATTACGAG AACCCACTAA AAAAGATGAT GGAGGAGTTT
    GTCCCTCATG GAAAATCGCT GTCAGATGCG CTGGTCTCCT TGCAGATGGT GTACCCACGC
    CGGAATCTGT CTGCAGATCA GTGGAGAAAC GCTCAGCTGC TCAGTTTGAT TAGCGCTCCC
    AGCACTATGC TCAACCCAGC TCAATCTGAC ACGATGCCCT GTGAATACCT ATCCTTGGAC
    ACCATGGAGA AATGGATCGT CTTCGGCTTC ATCTTGTGTC ATGCGGCTCT GAACAGTGAT
    CCTGCGGCTC TCAGTCTTTG GAAGTTGGCC CTTCAGAGCA GTACCTGTCT CTGTCTGTTC
    AGGGACGAGG TCTTCCACAT TCACAAAGCT GCAGAGGATC TCTTCGTAAA CATTCGCGGT
    TACAACAAAC GTGTCAATGA TATTCGTGAA TGCAAAGAAT CTGCTCTTTC ACATGCGGGC
    TCAATGCACA GAGAACGACG TAAGTTCTTG AGGTCTGCCC TGAAAGAGCT AGCCACAGTA
    CTGGCAGATC AGCCTGGATT GCTGGGTCCG AAGGCTCTGT TTGTGTTCAT GGCACTCTCC
    TTCGCCCGTG ATGAGATCAT TTGGCTGCTC AGACATGCCG ACAACATCCA AAAGAAAAGC
    ACAGATGACT TCATTGACAA GCACATTGCA GAGTTGATCT TCTACATGGA GGAGTTGAGG
    GCTCACGTCC GAAAGTACGG GCCGGTGATG CAGAGGTACT ATGTTCAGTA CCTGTCCGGC
    TTTGACGCGG TGGTGCTCAA TGAGCTTGTA CAGAACCTGT CCGTGTGCCC AGAGGACGAG
    TCCATCATAA TGTCGTCCTT CGTGAACACT ATGACCTCTC TCAGTGTTAA ACAAGTTGAA
    GATGGCGAAG TGTTTGATTT CAGAGGGATG AGGCTGGACT GGTTCAGACT TCAGGCCTAC
    ACCAGCGTGT CCAAGGCTTC GTTGGGACTG GCAGATCACA GAGAACTGGG AAAGATGATG
    AACACCATCA TCTTCCACAC TAAGATGGTG GACTCTCTGG TGGAGATGCT TGTGGAGACT
    TCTGACCTGT CCATATTCTG TTTTTACAGC CGCGCCTTTG AAAAGATGTT CCAGCAGTGT
    CTTGAACTGC CGTCCCAGTC TCGATACTCC ATCTCCTTCC CACTGCTCTG CACCCACTTT
    ATGAGCTGCA CTCATGAGCT CTGCCCTGAA GAGAGGCACC ACATCGGAGA CCGCAGCCTG
    TCCCTTTGCA ACATGTTCCT GGACGAGATG GCCAAGCAAG CCCGCAACCT CATCACAGAC
    ATATGCACAG AGCAGTGCAC CCTGAGCGAC CAGCTACTGC CAAAACATTG TGCCAAAACC
    ATCAGTCAGG CCGTGAACAA GAAGTCGAAA AAGCAGACAG GGAAGAAGGG TGAACCGGAG
    CGAGAAAAAC CTGGCGTGGA GAGCATGAGG AAGAACAGAC TCCTGGTCAC CAATCTGGAT
    AAACTGCACA CTGCTCTGTC CGAGCTCTGC TTCTCCATCA ACTACGTCCC CAACATGATG
    GTCTGGGAGC ACACCTTCAC CCCACGCGAG TACCTGACCT CCCACCTGGA GATTCGCTTC
    ACTAAGTCTA TAGTCGGGAT GACCATGTAC AACCAGACAA CACAGGAGAT CGCCAAACCA
    TCAGAGCTGC TCACCAGCGT CCGGGCCTAC ATGACAGTGC TGCAGTCCAT TGAGAATTAT
    GTGCAGATTG ACATCACCAG AGTCTTCAAC AATGTGCTGC TGCAGCAAAC CCAACACCTG
    GACAGCCACG GAGAGCCAAC CATCACCAGC CTCTACACTA ACTGGTATCT GGAGACTTTA
    CTGCGGCAGG TCAGCAACGG TCACATCGCG TACTTTCCTG CGATGAAAGC CTTCGTCAAC
    TTGCCCACGG AGAATGAGCT GACCTTCAAC GCAGAGGAGT ATTCTGACAT TTCTGAAATG
    CGCTCTCTGT CGGAGCTGCT GGGTCCATAT GGGATGAAGT TCCTCAGCGA GAGTCTCATG
    TGGCACATCT CATCCCAAGT TGCAGAACTG AAGAAACTTG TGGTGGACAA TGTGGAGGTT
    TTAACCCAGA TGAGGACCAG CTTTGACAAG CCGGACCATA TGGCAGCACT CTTTAAGAGG
    CTAACATCGG TTGACAGCGT GCTGAAACGG ATGACCATCA TTGGTGTGAT TTTGTCGTTC
    CGCTCTCTGG CTCAGGAAGC TCTCCGAGAT GTGCTCTCTT GCCACATTCC CTTCCTGGTG
    AGCTCCGTTG AAGACTTTAA AGATCACATT CCTCGTGAAA CTGACATGAA GGTGGCCATG
    AACGTGTACG AGCTGTCTTC TGCTGCAGGC CTGCCCTGTG AGATCGACCC TGCTCTAGTA
    GTGGCGCTGT CATCCCAGAA GTCTGAAAAT ATTAGCCCGG AGGAAGAGTA CAAGATCGCC
    TGCCTGTTGA TGGTCTTCGT GGCCGTTTCC ATGCCGACCC TGGCCAGCAA CGTGATGTCA
    CAGTACAGTC CCGCAATAGA AGGTCACTGC AACAACATTC ACTGTCTGGC TAAAGCGATA
    AACCAGATTG CCGCCGCTCT GTTCACGATC CACAAGGGCA GCATAGAGGA TCGCCTCAAA
    GAATTCCTCG CCCTCGCCTC CTCCAGCCTG CTGAAGATTG GACAGGAGAC GGATAAAACC
    ACTACACGGA ACAGAGAGTC TGTTTACCTG CTCCTGGACA TGATCGTTCA AGAATCGCCA
    TTCCTCACCA TGGACCTGTT GGAGTCCTGC TTTCCATACG TCCTGCTCCG AAACGCCTAT
    CACGCCGTAT ACAAGCAGTC GGTATCTTCT TCAGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15522
    Clone ID Related Accession (Same CDS sequence) XM_005167923.4 , XM_005167923.3
    Accession Version XM_005167923.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167923.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC AACAGCTTGC ACAACTACAA
    AAAGAAAAGT CAGAAATACT GAAGAATTTA GCTCTATATT ACTTCACCTT TGTGGACGTT
    ATGGAATTTA AGGACCACGT TTGTGAACTG CTGAACACTA TTGATGCTTG TCAAGTTTTC
    TTTGACATTA CTGTAAATTT TGACCTCACA AAGAACTACC TGGACTTGGT AGTGACGTAC
    ACTACCCTGA TGTTGCTGCT TTCCCGAATT GAAGAGCGCA AAGCGATAAT CGGCCTCTAC
    AACTATGCCC ATGAAATGAC ACACGGTTCA AGCGATCGAG AATACCCCAG GCTTGGACAG
    ATGATTGTGG ATTACGAGAA CCCACTAAAA AAGATGATGG AGGAGTTTGT CCCTCATGGA
    AAATCGCTGT CAGATGCGCT GGTCTCCTTG CAGATGGTGT ACCCACGCCG GAATCTGTCT
    GCAGATCAGT GGAGAAACGC TCAGCTGCTC AGTTTGATTA GCGCTCCCAG CACTATGCTC
    AACCCAGCTC AATCTGACAC GATGCCCTGT GAATACCTAT CCTTGGACAC CATGGAGAAA
    TGGATCGTCT TCGGCTTCAT CTTGTGTCAT GCGGCTCTGA ACAGTGATCC TGCGGCTCTC
    AGTCTTTGGA AGTTGGCCCT TCAGAGCAGT ACCTGTCTCT GTCTGTTCAG GGACGAGGTC
    TTCCACATTC ACAAAGCTGC AGAGGATCTC TTCGTAAACA TTCGCGGTTA CAACAAACGT
    GTCAATGATA TTCGTGAATG CAAAGAATCT GCTCTTTCAC ATGCGGGCTC AATGCACAGA
    GAACGACGTA AGTTCTTGAG GTCTGCCCTG AAAGAGCTAG CCACAGTACT GGCAGATCAG
    CCTGGATTGC TGGGTCCGAA GGCTCTGTTT GTGTTCATGG CACTCTCCTT CGCCCGTGAT
    GAGATCATTT GGCTGCTCAG ACATGCCGAC AACATCCAAA AGAAAAGCAC AGATGACTTC
    ATTGACAAGC ACATTGCAGA GTTGATCTTC TACATGGAGG AGTTGAGGGC TCACGTCCGA
    AAGTACGGGC CGGTGATGCA GAGGTACTAT GTTCAGTACC TGTCCGGCTT TGACGCGGTG
    GTGCTCAATG AGCTTGTACA GAACCTGTCC GTGTGCCCAG AGGACGAGTC CATCATAATG
    TCGTCCTTCG TGAACACTAT GACCTCTCTC AGTGTTAAAC AAGTTGAAGA TGGCGAAGTG
    TTTGATTTCA GAGGGATGAG GCTGGACTGG TTCAGACTTC AGGCCTACAC CAGCGTGTCC
    AAGGCTTCGT TGGGACTGGC AGATCACAGA GAACTGGGAA AGATGATGAA CACCATCATC
    TTCCACACTA AGATGGTGGA CTCTCTGGTG GAGATGCTTG TGGAGACTTC TGACCTGTCC
    ATATTCTGTT TTTACAGCCG CGCCTTTGAA AAGATGTTCC AGCAGTGTCT TGAACTGCCG
    TCCCAGTCTC GATACTCCAT CTCCTTCCCA CTGCTCTGCA CCCACTTTAT GAGCTGCACT
    CATGAGCTCT GCCCTGAAGA GAGGCACCAC ATCGGAGACC GCAGCCTGTC CCTTTGCAAC
    ATGTTCCTGG ACGAGATGGC CAAGCAAGCC CGCAACCTCA TCACAGACAT ATGCACAGAG
    CAGTGCACCC TGAGCGACCA GCTACTGCCA AAACATTGTG CCAAAACCAT CAGTCAGGCC
    GTGAACAAGA AGTCGAAAAA GCAGACAGGG AAGAAGGGTG AACCGGAGCG AGAAAAACCT
    GGCGTGGAGA GCATGAGGAA GAACAGACTC CTGGTCACCA ATCTGGATAA ACTGCACACT
    GCTCTGTCCG AGCTCTGCTT CTCCATCAAC TACGTCCCCA ACATGATGGT CTGGGAGCAC
    ACCTTCACCC CACGCGAGTA CCTGACCTCC CACCTGGAGA TTCGCTTCAC TAAGTCTATA
    GTCGGGATGA CCATGTACAA CCAGACAACA CAGGAGATCG CCAAACCATC AGAGCTGCTC
    ACCAGCGTCC GGGCCTACAT GACAGTGCTG CAGTCCATTG AGAATTATGT GCAGATTGAC
    ATCACCAGAG TCTTCAACAA TGTGCTGCTG CAGCAAACCC AACACCTGGA CAGCCACGGA
    GAGCCAACCA TCACCAGCCT CTACACTAAC TGGTATCTGG AGACTTTACT GCGGCAGGTC
    AGCAACGGTC ACATCGCGTA CTTTCCTGCG ATGAAAGCCT TCGTCAACTT GCCCACGGAG
    AATGAGCTGA CCTTCAACGC AGAGGAGTAT TCTGACATTT CTGAAATGCG CTCTCTGTCG
    GAGCTGCTGG GTCCATATGG GATGAAGTTC CTCAGCGAGA GTCTCATGTG GCACATCTCA
    TCCCAAGTTG CAGAACTGAA GAAACTTGTG GTGGACAATG TGGAGGTTTT AACCCAGATG
    AGGACCAGCT TTGACAAGCC GGACCATATG GCAGCACTCT TTAAGAGGCT AACATCGGTT
    GACAGCGTGC TGAAACGGAT GACCATCATT GGTGTGATTT TGTCGTTCCG CTCTCTGGCT
    CAGGAAGCTC TCCGAGATGT GCTCTCTTGC CACATTCCCT TCCTGGTGAG CTCCGTTGAA
    GACTTTAAAG ATCACATTCC TCGTGAAACT GACATGAAGG TGGCCATGAA CGTGTACGAG
    CTGTCTTCTG CTGCAGGCCT GCCCTGTGAG ATCGACCCTG CTCTAGTAGT GGCGCTGTCA
    TCCCAGAAGT CTGAAAATAT TAGCCCGGAG GAAGAGTACA AGATCGCCTG CCTGTTGATG
    GTCTTCGTGG CCGTTTCCAT GCCGACCCTG GCCAGCAACG TGATGTCACA GTACAGTCCC
    GCAATAGAAG GTCACTGCAA CAACATTCAC TGTCTGGCTA AAGCGATAAA CCAGATTGCC
    GCCGCTCTGT TCACGATCCA CAAGGGCAGC ATAGAGGATC GCCTCAAAGA ATTCCTCGCC
    CTCGCCTCCT CCAGCCTGCT GAAGATTGGA CAGGAGACGG ATAAAACCAC TACACGGAAC
    AGAGAGTCTG TTTACCTGCT CCTGGACATG ATCGTTCAAG AATCGCCATT CCTCACCATG
    GACCTGTTGG AGTCCTGCTT TCCATACGTC CTGCTCCGAA ACGCCTATCA CGCCGTATAC
    AAGCAGTCGG TATCTTCTTC AGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167980.1
    CDS212..3598
    Translation

    Target ORF information:

    RefSeq Version XM_005167923.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167923.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC AACAGCTTGC ACAACTACAA
    AAAGAAAAGT CAGAAATACT GAAGAATTTA GCTCTATATT ACTTCACCTT TGTGGACGTT
    ATGGAATTTA AGGACCACGT TTGTGAACTG CTGAACACTA TTGATGCTTG TCAAGTTTTC
    TTTGACATTA CTGTAAATTT TGACCTCACA AAGAACTACC TGGACTTGGT AGTGACGTAC
    ACTACCCTGA TGTTGCTGCT TTCCCGAATT GAAGAGCGCA AAGCGATAAT CGGCCTCTAC
    AACTATGCCC ATGAAATGAC ACACGGTTCA AGCGATCGAG AATACCCCAG GCTTGGACAG
    ATGATTGTGG ATTACGAGAA CCCACTAAAA AAGATGATGG AGGAGTTTGT CCCTCATGGA
    AAATCGCTGT CAGATGCGCT GGTCTCCTTG CAGATGGTGT ACCCACGCCG GAATCTGTCT
    GCAGATCAGT GGAGAAACGC TCAGCTGCTC AGTTTGATTA GCGCTCCCAG CACTATGCTC
    AACCCAGCTC AATCTGACAC GATGCCCTGT GAATACCTAT CCTTGGACAC CATGGAGAAA
    TGGATCGTCT TCGGCTTCAT CTTGTGTCAT GCGGCTCTGA ACAGTGATCC TGCGGCTCTC
    AGTCTTTGGA AGTTGGCCCT TCAGAGCAGT ACCTGTCTCT GTCTGTTCAG GGACGAGGTC
    TTCCACATTC ACAAAGCTGC AGAGGATCTC TTCGTAAACA TTCGCGGTTA CAACAAACGT
    GTCAATGATA TTCGTGAATG CAAAGAATCT GCTCTTTCAC ATGCGGGCTC AATGCACAGA
    GAACGACGTA AGTTCTTGAG GTCTGCCCTG AAAGAGCTAG CCACAGTACT GGCAGATCAG
    CCTGGATTGC TGGGTCCGAA GGCTCTGTTT GTGTTCATGG CACTCTCCTT CGCCCGTGAT
    GAGATCATTT GGCTGCTCAG ACATGCCGAC AACATCCAAA AGAAAAGCAC AGATGACTTC
    ATTGACAAGC ACATTGCAGA GTTGATCTTC TACATGGAGG AGTTGAGGGC TCACGTCCGA
    AAGTACGGGC CGGTGATGCA GAGGTACTAT GTTCAGTACC TGTCCGGCTT TGACGCGGTG
    GTGCTCAATG AGCTTGTACA GAACCTGTCC GTGTGCCCAG AGGACGAGTC CATCATAATG
    TCGTCCTTCG TGAACACTAT GACCTCTCTC AGTGTTAAAC AAGTTGAAGA TGGCGAAGTG
    TTTGATTTCA GAGGGATGAG GCTGGACTGG TTCAGACTTC AGGCCTACAC CAGCGTGTCC
    AAGGCTTCGT TGGGACTGGC AGATCACAGA GAACTGGGAA AGATGATGAA CACCATCATC
    TTCCACACTA AGATGGTGGA CTCTCTGGTG GAGATGCTTG TGGAGACTTC TGACCTGTCC
    ATATTCTGTT TTTACAGCCG CGCCTTTGAA AAGATGTTCC AGCAGTGTCT TGAACTGCCG
    TCCCAGTCTC GATACTCCAT CTCCTTCCCA CTGCTCTGCA CCCACTTTAT GAGCTGCACT
    CATGAGCTCT GCCCTGAAGA GAGGCACCAC ATCGGAGACC GCAGCCTGTC CCTTTGCAAC
    ATGTTCCTGG ACGAGATGGC CAAGCAAGCC CGCAACCTCA TCACAGACAT ATGCACAGAG
    CAGTGCACCC TGAGCGACCA GCTACTGCCA AAACATTGTG CCAAAACCAT CAGTCAGGCC
    GTGAACAAGA AGTCGAAAAA GCAGACAGGG AAGAAGGGTG AACCGGAGCG AGAAAAACCT
    GGCGTGGAGA GCATGAGGAA GAACAGACTC CTGGTCACCA ATCTGGATAA ACTGCACACT
    GCTCTGTCCG AGCTCTGCTT CTCCATCAAC TACGTCCCCA ACATGATGGT CTGGGAGCAC
    ACCTTCACCC CACGCGAGTA CCTGACCTCC CACCTGGAGA TTCGCTTCAC TAAGTCTATA
    GTCGGGATGA CCATGTACAA CCAGACAACA CAGGAGATCG CCAAACCATC AGAGCTGCTC
    ACCAGCGTCC GGGCCTACAT GACAGTGCTG CAGTCCATTG AGAATTATGT GCAGATTGAC
    ATCACCAGAG TCTTCAACAA TGTGCTGCTG CAGCAAACCC AACACCTGGA CAGCCACGGA
    GAGCCAACCA TCACCAGCCT CTACACTAAC TGGTATCTGG AGACTTTACT GCGGCAGGTC
    AGCAACGGTC ACATCGCGTA CTTTCCTGCG ATGAAAGCCT TCGTCAACTT GCCCACGGAG
    AATGAGCTGA CCTTCAACGC AGAGGAGTAT TCTGACATTT CTGAAATGCG CTCTCTGTCG
    GAGCTGCTGG GTCCATATGG GATGAAGTTC CTCAGCGAGA GTCTCATGTG GCACATCTCA
    TCCCAAGTTG CAGAACTGAA GAAACTTGTG GTGGACAATG TGGAGGTTTT AACCCAGATG
    AGGACCAGCT TTGACAAGCC GGACCATATG GCAGCACTCT TTAAGAGGCT AACATCGGTT
    GACAGCGTGC TGAAACGGAT GACCATCATT GGTGTGATTT TGTCGTTCCG CTCTCTGGCT
    CAGGAAGCTC TCCGAGATGT GCTCTCTTGC CACATTCCCT TCCTGGTGAG CTCCGTTGAA
    GACTTTAAAG ATCACATTCC TCGTGAAACT GACATGAAGG TGGCCATGAA CGTGTACGAG
    CTGTCTTCTG CTGCAGGCCT GCCCTGTGAG ATCGACCCTG CTCTAGTAGT GGCGCTGTCA
    TCCCAGAAGT CTGAAAATAT TAGCCCGGAG GAAGAGTACA AGATCGCCTG CCTGTTGATG
    GTCTTCGTGG CCGTTTCCAT GCCGACCCTG GCCAGCAACG TGATGTCACA GTACAGTCCC
    GCAATAGAAG GTCACTGCAA CAACATTCAC TGTCTGGCTA AAGCGATAAA CCAGATTGCC
    GCCGCTCTGT TCACGATCCA CAAGGGCAGC ATAGAGGATC GCCTCAAAGA ATTCCTCGCC
    CTCGCCTCCT CCAGCCTGCT GAAGATTGGA CAGGAGACGG ATAAAACCAC TACACGGAAC
    AGAGAGTCTG TTTACCTGCT CCTGGACATG ATCGTTCAAG AATCGCCATT CCTCACCATG
    GACCTGTTGG AGTCCTGCTT TCCATACGTC CTGCTCCGAA ACGCCTATCA CGCCGTATAC
    AAGCAGTCGG TATCTTCTTC AGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15522
    Clone ID Related Accession (Same CDS sequence) XM_005167923.4 , XM_005167923.3
    Accession Version XM_005167923.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nck-associated protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC AACAGCTTGC ACAACTACAA
    AAAGAAAAGT CAGAAATACT GAAGAATTTA GCTCTATATT ACTTCACCTT TGTGGACGTT
    ATGGAATTTA AGGACCACGT TTGTGAACTG CTGAACACTA TTGATGCTTG TCAAGTTTTC
    TTTGACATTA CTGTAAATTT TGACCTCACA AAGAACTACC TGGACTTGGT AGTGACGTAC
    ACTACCCTGA TGTTGCTGCT TTCCCGAATT GAAGAGCGCA AAGCGATAAT CGGCCTCTAC
    AACTATGCCC ATGAAATGAC ACACGGTTCA AGCGATCGAG AATACCCCAG GCTTGGACAG
    ATGATTGTGG ATTACGAGAA CCCACTAAAA AAGATGATGG AGGAGTTTGT CCCTCATGGA
    AAATCGCTGT CAGATGCGCT GGTCTCCTTG CAGATGGTGT ACCCACGCCG GAATCTGTCT
    GCAGATCAGT GGAGAAACGC TCAGCTGCTC AGTTTGATTA GCGCTCCCAG CACTATGCTC
    AACCCAGCTC AATCTGACAC GATGCCCTGT GAATACCTAT CCTTGGACAC CATGGAGAAA
    TGGATCGTCT TCGGCTTCAT CTTGTGTCAT GCGGCTCTGA ACAGTGATCC TGCGGCTCTC
    AGTCTTTGGA AGTTGGCCCT TCAGAGCAGT ACCTGTCTCT GTCTGTTCAG GGACGAGGTC
    TTCCACATTC ACAAAGCTGC AGAGGATCTC TTCGTAAACA TTCGCGGTTA CAACAAACGT
    GTCAATGATA TTCGTGAATG CAAAGAATCT GCTCTTTCAC ATGCGGGCTC AATGCACAGA
    GAACGACGTA AGTTCTTGAG GTCTGCCCTG AAAGAGCTAG CCACAGTACT GGCAGATCAG
    CCTGGATTGC TGGGTCCGAA GGCTCTGTTT GTGTTCATGG CACTCTCCTT CGCCCGTGAT
    GAGATCATTT GGCTGCTCAG ACATGCCGAC AACATCCAAA AGAAAAGCAC AGATGACTTC
    ATTGACAAGC ACATTGCAGA GTTGATCTTC TACATGGAGG AGTTGAGGGC TCACGTCCGA
    AAGTACGGGC CGGTGATGCA GAGGTACTAT GTTCAGTACC TGTCCGGCTT TGACGCGGTG
    GTGCTCAATG AGCTTGTACA GAACCTGTCC GTGTGCCCAG AGGACGAGTC CATCATAATG
    TCGTCCTTCG TGAACACTAT GACCTCTCTC AGTGTTAAAC AAGTTGAAGA TGGCGAAGTG
    TTTGATTTCA GAGGGATGAG GCTGGACTGG TTCAGACTTC AGGCCTACAC CAGCGTGTCC
    AAGGCTTCGT TGGGACTGGC AGATCACAGA GAACTGGGAA AGATGATGAA CACCATCATC
    TTCCACACTA AGATGGTGGA CTCTCTGGTG GAGATGCTTG TGGAGACTTC TGACCTGTCC
    ATATTCTGTT TTTACAGCCG CGCCTTTGAA AAGATGTTCC AGCAGTGTCT TGAACTGCCG
    TCCCAGTCTC GATACTCCAT CTCCTTCCCA CTGCTCTGCA CCCACTTTAT GAGCTGCACT
    CATGAGCTCT GCCCTGAAGA GAGGCACCAC ATCGGAGACC GCAGCCTGTC CCTTTGCAAC
    ATGTTCCTGG ACGAGATGGC CAAGCAAGCC CGCAACCTCA TCACAGACAT ATGCACAGAG
    CAGTGCACCC TGAGCGACCA GCTACTGCCA AAACATTGTG CCAAAACCAT CAGTCAGGCC
    GTGAACAAGA AGTCGAAAAA GCAGACAGGG AAGAAGGGTG AACCGGAGCG AGAAAAACCT
    GGCGTGGAGA GCATGAGGAA GAACAGACTC CTGGTCACCA ATCTGGATAA ACTGCACACT
    GCTCTGTCCG AGCTCTGCTT CTCCATCAAC TACGTCCCCA ACATGATGGT CTGGGAGCAC
    ACCTTCACCC CACGCGAGTA CCTGACCTCC CACCTGGAGA TTCGCTTCAC TAAGTCTATA
    GTCGGGATGA CCATGTACAA CCAGACAACA CAGGAGATCG CCAAACCATC AGAGCTGCTC
    ACCAGCGTCC GGGCCTACAT GACAGTGCTG CAGTCCATTG AGAATTATGT GCAGATTGAC
    ATCACCAGAG TCTTCAACAA TGTGCTGCTG CAGCAAACCC AACACCTGGA CAGCCACGGA
    GAGCCAACCA TCACCAGCCT CTACACTAAC TGGTATCTGG AGACTTTACT GCGGCAGGTC
    AGCAACGGTC ACATCGCGTA CTTTCCTGCG ATGAAAGCCT TCGTCAACTT GCCCACGGAG
    AATGAGCTGA CCTTCAACGC AGAGGAGTAT TCTGACATTT CTGAAATGCG CTCTCTGTCG
    GAGCTGCTGG GTCCATATGG GATGAAGTTC CTCAGCGAGA GTCTCATGTG GCACATCTCA
    TCCCAAGTTG CAGAACTGAA GAAACTTGTG GTGGACAATG TGGAGGTTTT AACCCAGATG
    AGGACCAGCT TTGACAAGCC GGACCATATG GCAGCACTCT TTAAGAGGCT AACATCGGTT
    GACAGCGTGC TGAAACGGAT GACCATCATT GGTGTGATTT TGTCGTTCCG CTCTCTGGCT
    CAGGAAGCTC TCCGAGATGT GCTCTCTTGC CACATTCCCT TCCTGGTGAG CTCCGTTGAA
    GACTTTAAAG ATCACATTCC TCGTGAAACT GACATGAAGG TGGCCATGAA CGTGTACGAG
    CTGTCTTCTG CTGCAGGCCT GCCCTGTGAG ATCGACCCTG CTCTAGTAGT GGCGCTGTCA
    TCCCAGAAGT CTGAAAATAT TAGCCCGGAG GAAGAGTACA AGATCGCCTG CCTGTTGATG
    GTCTTCGTGG CCGTTTCCAT GCCGACCCTG GCCAGCAACG TGATGTCACA GTACAGTCCC
    GCAATAGAAG GTCACTGCAA CAACATTCAC TGTCTGGCTA AAGCGATAAA CCAGATTGCC
    GCCGCTCTGT TCACGATCCA CAAGGGCAGC ATAGAGGATC GCCTCAAAGA ATTCCTCGCC
    CTCGCCTCCT CCAGCCTGCT GAAGATTGGA CAGGAGACGG ATAAAACCAC TACACGGAAC
    AGAGAGTCTG TTTACCTGCT CCTGGACATG ATCGTTCAAG AATCGCCATT CCTCACCATG
    GACCTGTTGG AGTCCTGCTT TCCATACGTC CTGCTCCGAA ACGCCTATCA CGCCGTATAC
    AAGCAGTCGG TATCTTCTTC AGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167980.1
    CDS231..3617
    Translation

    Target ORF information:

    RefSeq Version XM_005167923.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167923.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC AACAGCTTGC ACAACTACAA
    AAAGAAAAGT CAGAAATACT GAAGAATTTA GCTCTATATT ACTTCACCTT TGTGGACGTT
    ATGGAATTTA AGGACCACGT TTGTGAACTG CTGAACACTA TTGATGCTTG TCAAGTTTTC
    TTTGACATTA CTGTAAATTT TGACCTCACA AAGAACTACC TGGACTTGGT AGTGACGTAC
    ACTACCCTGA TGTTGCTGCT TTCCCGAATT GAAGAGCGCA AAGCGATAAT CGGCCTCTAC
    AACTATGCCC ATGAAATGAC ACACGGTTCA AGCGATCGAG AATACCCCAG GCTTGGACAG
    ATGATTGTGG ATTACGAGAA CCCACTAAAA AAGATGATGG AGGAGTTTGT CCCTCATGGA
    AAATCGCTGT CAGATGCGCT GGTCTCCTTG CAGATGGTGT ACCCACGCCG GAATCTGTCT
    GCAGATCAGT GGAGAAACGC TCAGCTGCTC AGTTTGATTA GCGCTCCCAG CACTATGCTC
    AACCCAGCTC AATCTGACAC GATGCCCTGT GAATACCTAT CCTTGGACAC CATGGAGAAA
    TGGATCGTCT TCGGCTTCAT CTTGTGTCAT GCGGCTCTGA ACAGTGATCC TGCGGCTCTC
    AGTCTTTGGA AGTTGGCCCT TCAGAGCAGT ACCTGTCTCT GTCTGTTCAG GGACGAGGTC
    TTCCACATTC ACAAAGCTGC AGAGGATCTC TTCGTAAACA TTCGCGGTTA CAACAAACGT
    GTCAATGATA TTCGTGAATG CAAAGAATCT GCTCTTTCAC ATGCGGGCTC AATGCACAGA
    GAACGACGTA AGTTCTTGAG GTCTGCCCTG AAAGAGCTAG CCACAGTACT GGCAGATCAG
    CCTGGATTGC TGGGTCCGAA GGCTCTGTTT GTGTTCATGG CACTCTCCTT CGCCCGTGAT
    GAGATCATTT GGCTGCTCAG ACATGCCGAC AACATCCAAA AGAAAAGCAC AGATGACTTC
    ATTGACAAGC ACATTGCAGA GTTGATCTTC TACATGGAGG AGTTGAGGGC TCACGTCCGA
    AAGTACGGGC CGGTGATGCA GAGGTACTAT GTTCAGTACC TGTCCGGCTT TGACGCGGTG
    GTGCTCAATG AGCTTGTACA GAACCTGTCC GTGTGCCCAG AGGACGAGTC CATCATAATG
    TCGTCCTTCG TGAACACTAT GACCTCTCTC AGTGTTAAAC AAGTTGAAGA TGGCGAAGTG
    TTTGATTTCA GAGGGATGAG GCTGGACTGG TTCAGACTTC AGGCCTACAC CAGCGTGTCC
    AAGGCTTCGT TGGGACTGGC AGATCACAGA GAACTGGGAA AGATGATGAA CACCATCATC
    TTCCACACTA AGATGGTGGA CTCTCTGGTG GAGATGCTTG TGGAGACTTC TGACCTGTCC
    ATATTCTGTT TTTACAGCCG CGCCTTTGAA AAGATGTTCC AGCAGTGTCT TGAACTGCCG
    TCCCAGTCTC GATACTCCAT CTCCTTCCCA CTGCTCTGCA CCCACTTTAT GAGCTGCACT
    CATGAGCTCT GCCCTGAAGA GAGGCACCAC ATCGGAGACC GCAGCCTGTC CCTTTGCAAC
    ATGTTCCTGG ACGAGATGGC CAAGCAAGCC CGCAACCTCA TCACAGACAT ATGCACAGAG
    CAGTGCACCC TGAGCGACCA GCTACTGCCA AAACATTGTG CCAAAACCAT CAGTCAGGCC
    GTGAACAAGA AGTCGAAAAA GCAGACAGGG AAGAAGGGTG AACCGGAGCG AGAAAAACCT
    GGCGTGGAGA GCATGAGGAA GAACAGACTC CTGGTCACCA ATCTGGATAA ACTGCACACT
    GCTCTGTCCG AGCTCTGCTT CTCCATCAAC TACGTCCCCA ACATGATGGT CTGGGAGCAC
    ACCTTCACCC CACGCGAGTA CCTGACCTCC CACCTGGAGA TTCGCTTCAC TAAGTCTATA
    GTCGGGATGA CCATGTACAA CCAGACAACA CAGGAGATCG CCAAACCATC AGAGCTGCTC
    ACCAGCGTCC GGGCCTACAT GACAGTGCTG CAGTCCATTG AGAATTATGT GCAGATTGAC
    ATCACCAGAG TCTTCAACAA TGTGCTGCTG CAGCAAACCC AACACCTGGA CAGCCACGGA
    GAGCCAACCA TCACCAGCCT CTACACTAAC TGGTATCTGG AGACTTTACT GCGGCAGGTC
    AGCAACGGTC ACATCGCGTA CTTTCCTGCG ATGAAAGCCT TCGTCAACTT GCCCACGGAG
    AATGAGCTGA CCTTCAACGC AGAGGAGTAT TCTGACATTT CTGAAATGCG CTCTCTGTCG
    GAGCTGCTGG GTCCATATGG GATGAAGTTC CTCAGCGAGA GTCTCATGTG GCACATCTCA
    TCCCAAGTTG CAGAACTGAA GAAACTTGTG GTGGACAATG TGGAGGTTTT AACCCAGATG
    AGGACCAGCT TTGACAAGCC GGACCATATG GCAGCACTCT TTAAGAGGCT AACATCGGTT
    GACAGCGTGC TGAAACGGAT GACCATCATT GGTGTGATTT TGTCGTTCCG CTCTCTGGCT
    CAGGAAGCTC TCCGAGATGT GCTCTCTTGC CACATTCCCT TCCTGGTGAG CTCCGTTGAA
    GACTTTAAAG ATCACATTCC TCGTGAAACT GACATGAAGG TGGCCATGAA CGTGTACGAG
    CTGTCTTCTG CTGCAGGCCT GCCCTGTGAG ATCGACCCTG CTCTAGTAGT GGCGCTGTCA
    TCCCAGAAGT CTGAAAATAT TAGCCCGGAG GAAGAGTACA AGATCGCCTG CCTGTTGATG
    GTCTTCGTGG CCGTTTCCAT GCCGACCCTG GCCAGCAACG TGATGTCACA GTACAGTCCC
    GCAATAGAAG GTCACTGCAA CAACATTCAC TGTCTGGCTA AAGCGATAAA CCAGATTGCC
    GCCGCTCTGT TCACGATCCA CAAGGGCAGC ATAGAGGATC GCCTCAAAGA ATTCCTCGCC
    CTCGCCTCCT CCAGCCTGCT GAAGATTGGA CAGGAGACGG ATAAAACCAC TACACGGAAC
    AGAGAGTCTG TTTACCTGCT CCTGGACATG ATCGTTCAAG AATCGCCATT CCTCACCATG
    GACCTGTTGG AGTCCTGCTT TCCATACGTC CTGCTCCGAA ACGCCTATCA CGCCGTATAC
    AAGCAGTCGG TATCTTCTTC AGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15523
    Clone ID Related Accession (Same CDS sequence) XM_005167924.3 , XM_005167924.4
    Accession Version XM_005167924.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nck-associated protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA
    AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA
    TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC
    ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC
    CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT
    GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT
    GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG
    CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT
    CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA
    GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC
    GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT
    TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC
    ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT
    GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA
    CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA
    TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC
    ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC
    AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC
    GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC
    AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC
    TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT
    TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT
    TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC
    ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC
    TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG
    TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG
    CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC
    CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC
    ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC
    AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG
    GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG
    TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC
    ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG
    ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC
    GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC
    AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA
    ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC
    GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG
    CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG
    CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA
    GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC
    AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC
    GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA
    GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT
    AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT
    TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG
    AAGTCTGAAA ATATTAGCCC GGAGGAAGAG TACAAGATCG CCTGCCTGTT GATGGTCTTC
    GTGGCCGTTT CCATGCCGAC CCTGGCCAGC AACGTGATGT CACAGTACAG TCCCGCAATA
    GAAGGTCACT GCAACAACAT TCACTGTCTG GCTAAAGCGA TAAACCAGAT TGCCGCCGCT
    CTGTTCACGA TCCACAAGGG CAGCATAGAG GATCGCCTCA AAGAATTCCT CGCCCTCGCC
    TCCTCCAGCC TGCTGAAGAT TGGACAGGAG ACGGATAAAA CCACTACACG GAACAGAGAG
    TCTGTTTACC TGCTCCTGGA CATGATCGTT CAAGAATCGC CATTCCTCAC CATGGACCTG
    TTGGAGTCCT GCTTTCCATA CGTCCTGCTC CGAAACGCCT ATCACGCCGT ATACAAGCAG
    TCGGTATCTT CTTCAGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167981.1
    CDS230..3610
    Translation

    Target ORF information:

    RefSeq Version XM_005167924.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167924.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA
    AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA
    TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC
    ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC
    CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT
    GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT
    GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG
    CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT
    CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA
    GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC
    GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT
    TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC
    ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT
    GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA
    CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA
    TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC
    ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC
    AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC
    GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC
    AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC
    TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT
    TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT
    TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC
    ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC
    TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG
    TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG
    CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC
    CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC
    ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC
    AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG
    GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG
    TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC
    ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG
    ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC
    GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC
    AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA
    ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC
    GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG
    CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG
    CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA
    GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC
    AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC
    GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA
    GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT
    AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT
    TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG
    AAGTCTGAAA ATATTAGCCC GGAGGAAGAG TACAAGATCG CCTGCCTGTT GATGGTCTTC
    GTGGCCGTTT CCATGCCGAC CCTGGCCAGC AACGTGATGT CACAGTACAG TCCCGCAATA
    GAAGGTCACT GCAACAACAT TCACTGTCTG GCTAAAGCGA TAAACCAGAT TGCCGCCGCT
    CTGTTCACGA TCCACAAGGG CAGCATAGAG GATCGCCTCA AAGAATTCCT CGCCCTCGCC
    TCCTCCAGCC TGCTGAAGAT TGGACAGGAG ACGGATAAAA CCACTACACG GAACAGAGAG
    TCTGTTTACC TGCTCCTGGA CATGATCGTT CAAGAATCGC CATTCCTCAC CATGGACCTG
    TTGGAGTCCT GCTTTCCATA CGTCCTGCTC CGAAACGCCT ATCACGCCGT ATACAAGCAG
    TCGGTATCTT CTTCAGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15523
    Clone ID Related Accession (Same CDS sequence) XM_005167924.3 , XM_005167924.4
    Accession Version XM_005167924.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167924.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA
    AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA
    TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC
    ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC
    CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT
    GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT
    GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG
    CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT
    CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA
    GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC
    GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT
    TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC
    ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT
    GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA
    CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA
    TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC
    ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC
    AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC
    GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC
    AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC
    TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT
    TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT
    TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC
    ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC
    TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG
    TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG
    CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC
    CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC
    ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC
    AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG
    GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG
    TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC
    ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG
    ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC
    GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC
    AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA
    ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC
    GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG
    CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG
    CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA
    GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC
    AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC
    GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA
    GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT
    AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT
    TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG
    AAGTCTGAAA ATATTAGCCC GGAGGAAGAG TACAAGATCG CCTGCCTGTT GATGGTCTTC
    GTGGCCGTTT CCATGCCGAC CCTGGCCAGC AACGTGATGT CACAGTACAG TCCCGCAATA
    GAAGGTCACT GCAACAACAT TCACTGTCTG GCTAAAGCGA TAAACCAGAT TGCCGCCGCT
    CTGTTCACGA TCCACAAGGG CAGCATAGAG GATCGCCTCA AAGAATTCCT CGCCCTCGCC
    TCCTCCAGCC TGCTGAAGAT TGGACAGGAG ACGGATAAAA CCACTACACG GAACAGAGAG
    TCTGTTTACC TGCTCCTGGA CATGATCGTT CAAGAATCGC CATTCCTCAC CATGGACCTG
    TTGGAGTCCT GCTTTCCATA CGTCCTGCTC CGAAACGCCT ATCACGCCGT ATACAAGCAG
    TCGGTATCTT CTTCAGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167981.1
    CDS212..3592
    Translation

    Target ORF information:

    RefSeq Version XM_005167924.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167924.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
    AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT
    CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT
    GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA
    AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA
    TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC
    ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC
    CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT
    GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT
    GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG
    CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT
    CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA
    GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC
    GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT
    TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC
    ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT
    GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA
    CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA
    TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC
    ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC
    AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC
    GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC
    AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC
    TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT
    TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT
    TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC
    ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC
    TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG
    TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG
    CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC
    CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC
    ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC
    AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG
    GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG
    TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC
    ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG
    ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC
    GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC
    AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA
    ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC
    GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG
    CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG
    CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA
    GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC
    AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC
    GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA
    GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT
    AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT
    TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG
    AAGTCTGAAA ATATTAGCCC GGAGGAAGAG TACAAGATCG CCTGCCTGTT GATGGTCTTC
    GTGGCCGTTT CCATGCCGAC CCTGGCCAGC AACGTGATGT CACAGTACAG TCCCGCAATA
    GAAGGTCACT GCAACAACAT TCACTGTCTG GCTAAAGCGA TAAACCAGAT TGCCGCCGCT
    CTGTTCACGA TCCACAAGGG CAGCATAGAG GATCGCCTCA AAGAATTCCT CGCCCTCGCC
    TCCTCCAGCC TGCTGAAGAT TGGACAGGAG ACGGATAAAA CCACTACACG GAACAGAGAG
    TCTGTTTACC TGCTCCTGGA CATGATCGTT CAAGAATCGC CATTCCTCAC CATGGACCTG
    TTGGAGTCCT GCTTTCCATA CGTCCTGCTC CGAAACGCCT ATCACGCCGT ATACAAGCAG
    TCGGTATCTT CTTCAGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.