NCKAP1 gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_005167922.4	XP_005167979.1	nck-associated protein 1 isoform X2
XM_005167924.3	XP_005167981.1	nck-associated protein 1 isoform X4
NM_001137665.1	NP_001131137.1	nck-associated protein 1
XM_005167923.4	XP_005167980.1	nck-associated protein 1 isoform X3
XM_005167924.4	XP_005167981.1	nck-associated protein 1 isoform X4
XM_005167921.4	XP_005167978.1	nck-associated protein 1 isoform X1
XM_005167921.3	XP_005167978.1	nck-associated protein 1 isoform X1
XM_005167923.3	XP_005167980.1	nck-associated protein 1 isoform X3
XM_005167922.3	XP_005167979.1	nck-associated protein 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02048	NM_001137665.1 Latest version!	Danio rerio NCK-associated protein 1 (nckap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15520	XM_005167921.4 Latest version!	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15521	XM_005167922.4 Latest version!	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15522	XM_005167923.4 Latest version!	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15523	XM_005167924.4 Latest version!	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa15523	XM_005167924.3	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15520	XM_005167921.3	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15522	XM_005167923.3	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15521	XM_005167922.3	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

NCKAP1 ( NM_001137665.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001131137.1 Danio rerio NCK-associated protein 1 (nckap1), mRNA.

Price & Availability↑

CloneID	ODa02048
Clone ID Related Accession (Same CDS sequence)	NM_001137665.1
Accession Version	NM_001137665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Danio rerio(zebrafish)
Product	nck-associated protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX548025.9 and CR513785.10. On Nov 13, 2008 this sequence version replaced XM_001332244.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA 
AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA 
TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC 
ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC 
CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT 
GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT 
GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG 
CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT 
CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA 
GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC 
GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT 
TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC 
ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT 
GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA 
CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA 
TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC 
ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC 
AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC 
GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC 
AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC 
TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT 
TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT 
TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC 
ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC 
TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG 
TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG 
CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC 
CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC 
ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC 
AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG 
GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG 
TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC 
ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG 
ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC 
GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC 
AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA 
ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC 
GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG 
CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG 
CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA 
GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC 
AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC 
GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA 
GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT 
AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT 
TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG 
AAGTCTGGTA ACCCGGAGGA AGAGTACAAG ATCGCCTGCC TGTTGATGGT CTTCGTGGCC 
GTTTCCATGC CGACCCTGGC CAGCAACGTG ATGTCACAGT ACAGTCCCGC AATAGAAGGT 
CACTGCAACA ACATTCACTG TCTGGCTAAA GCGATAAACC AGATTGCCGC CGCTCTGTTC 
ACGATCCACA AGGGCAGCAT AGAGGATCGC CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC 
AGCCTGCTGA AGATTGGACA GGAGACGGAT AAAACCACTA CACGGAACAG AGAGTCTGTT 
TACCTGCTCC TGGACATGAT CGTTCAAGAA TCGCCATTCC TCACCATGGA CCTGTTGGAG 
TCCTGCTTTC CATACGTCCT GCTCCGAAAC GCCTATCACG CCGTATACAA GCAGTCGGTA 
TCTTCTTCAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131137.1
CDS	217..3591
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSGNPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	NM_001137665.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137665.1

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA 
AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA 
TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC 
ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC 
CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT 
GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT 
GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG 
CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT 
CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA 
GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC 
GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT 
TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC 
ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT 
GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA 
CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA 
TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC 
ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC 
AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC 
GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC 
AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC 
TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT 
TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT 
TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC 
ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC 
TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG 
TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG 
CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC 
CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC 
ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC 
AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG 
GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG 
TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC 
ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG 
ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC 
GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC 
AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA 
ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC 
GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG 
CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG 
CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA 
GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC 
AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC 
GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA 
GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT 
AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT 
TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG 
AAGTCTGGTA ACCCGGAGGA AGAGTACAAG ATCGCCTGCC TGTTGATGGT CTTCGTGGCC 
GTTTCCATGC CGACCCTGGC CAGCAACGTG ATGTCACAGT ACAGTCCCGC AATAGAAGGT 
CACTGCAACA ACATTCACTG TCTGGCTAAA GCGATAAACC AGATTGCCGC CGCTCTGTTC 
ACGATCCACA AGGGCAGCAT AGAGGATCGC CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC 
AGCCTGCTGA AGATTGGACA GGAGACGGAT AAAACCACTA CACGGAACAG AGAGTCTGTT 
TACCTGCTCC TGGACATGAT CGTTCAAGAA TCGCCATTCC TCACCATGGA CCTGTTGGAG 
TCCTGCTTTC CATACGTCCT GCTCCGAAAC GCCTATCACG CCGTATACAA GCAGTCGGTA 
TCTTCTTCAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_005167921.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167978.1 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15520
Clone ID Related Accession (Same CDS sequence)	XM_005167921.4 , XM_005167921.3
Accession Version	XM_005167921.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nck-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167921.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT 
TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG 
TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAAAA AGAAAAGTCA GAAATACTGA AGAATTTAGC TCTATATTAC 
TTCACCTTTG TGGACGTTAT GGAATTTAAG GACCACGTTT GTGAACTGCT GAACACTATT 
GATGCTTGTC AAGTTTTCTT TGACATTACT GTAAATTTTG ACCTCACAAA GAACTACCTG 
GACTTGGTAG TGACGTACAC TACCCTGATG TTGCTGCTTT CCCGAATTGA AGAGCGCAAA 
GCGATAATCG GCCTCTACAA CTATGCCCAT GAAATGACAC ACGGTTCAAG CGATCGAGAA 
TACCCCAGGC TTGGACAGAT GATTGTGGAT TACGAGAACC CACTAAAAAA GATGATGGAG 
GAGTTTGTCC CTCATGGAAA ATCGCTGTCA GATGCGCTGG TCTCCTTGCA GATGGTGTAC 
CCACGCCGGA ATCTGTCTGC AGATCAGTGG AGAAACGCTC AGCTGCTCAG TTTGATTAGC 
GCTCCCAGCA CTATGCTCAA CCCAGCTCAA TCTGACACGA TGCCCTGTGA ATACCTATCC 
TTGGACACCA TGGAGAAATG GATCGTCTTC GGCTTCATCT TGTGTCATGC GGCTCTGAAC 
AGTGATCCTG CGGCTCTCAG TCTTTGGAAG TTGGCCCTTC AGAGCAGTAC CTGTCTCTGT 
CTGTTCAGGG ACGAGGTCTT CCACATTCAC AAAGCTGCAG AGGATCTCTT CGTAAACATT 
CGCGGTTACA ACAAACGTGT CAATGATATT CGTGAATGCA AAGAATCTGC TCTTTCACAT 
GCGGGCTCAA TGCACAGAGA ACGACGTAAG TTCTTGAGGT CTGCCCTGAA AGAGCTAGCC 
ACAGTACTGG CAGATCAGCC TGGATTGCTG GGTCCGAAGG CTCTGTTTGT GTTCATGGCA 
CTCTCCTTCG CCCGTGATGA GATCATTTGG CTGCTCAGAC ATGCCGACAA CATCCAAAAG 
AAAAGCACAG ATGACTTCAT TGACAAGCAC ATTGCAGAGT TGATCTTCTA CATGGAGGAG 
TTGAGGGCTC ACGTCCGAAA GTACGGGCCG GTGATGCAGA GGTACTATGT TCAGTACCTG 
TCCGGCTTTG ACGCGGTGGT GCTCAATGAG CTTGTACAGA ACCTGTCCGT GTGCCCAGAG 
GACGAGTCCA TCATAATGTC GTCCTTCGTG AACACTATGA CCTCTCTCAG TGTTAAACAA 
GTTGAAGATG GCGAAGTGTT TGATTTCAGA GGGATGAGGC TGGACTGGTT CAGACTTCAG 
GCCTACACCA GCGTGTCCAA GGCTTCGTTG GGACTGGCAG ATCACAGAGA ACTGGGAAAG 
ATGATGAACA CCATCATCTT CCACACTAAG ATGGTGGACT CTCTGGTGGA GATGCTTGTG 
GAGACTTCTG ACCTGTCCAT ATTCTGTTTT TACAGCCGCG CCTTTGAAAA GATGTTCCAG 
CAGTGTCTTG AACTGCCGTC CCAGTCTCGA TACTCCATCT CCTTCCCACT GCTCTGCACC 
CACTTTATGA GCTGCACTCA TGAGCTCTGC CCTGAAGAGA GGCACCACAT CGGAGACCGC 
AGCCTGTCCC TTTGCAACAT GTTCCTGGAC GAGATGGCCA AGCAAGCCCG CAACCTCATC 
ACAGACATAT GCACAGAGCA GTGCACCCTG AGCGACCAGC TACTGCCAAA ACATTGTGCC 
AAAACCATCA GTCAGGCCGT GAACAAGAAG TCGAAAAAGC AGACAGGGAA GAAGGGTGAA 
CCGGAGCGAG AAAAACCTGG CGTGGAGAGC ATGAGGAAGA ACAGACTCCT GGTCACCAAT 
CTGGATAAAC TGCACACTGC TCTGTCCGAG CTCTGCTTCT CCATCAACTA CGTCCCCAAC 
ATGATGGTCT GGGAGCACAC CTTCACCCCA CGCGAGTACC TGACCTCCCA CCTGGAGATT 
CGCTTCACTA AGTCTATAGT CGGGATGACC ATGTACAACC AGACAACACA GGAGATCGCC 
AAACCATCAG AGCTGCTCAC CAGCGTCCGG GCCTACATGA CAGTGCTGCA GTCCATTGAG 
AATTATGTGC AGATTGACAT CACCAGAGTC TTCAACAATG TGCTGCTGCA GCAAACCCAA 
CACCTGGACA GCCACGGAGA GCCAACCATC ACCAGCCTCT ACACTAACTG GTATCTGGAG 
ACTTTACTGC GGCAGGTCAG CAACGGTCAC ATCGCGTACT TTCCTGCGAT GAAAGCCTTC 
GTCAACTTGC CCACGGAGAA TGAGCTGACC TTCAACGCAG AGGAGTATTC TGACATTTCT 
GAAATGCGCT CTCTGTCGGA GCTGCTGGGT CCATATGGGA TGAAGTTCCT CAGCGAGAGT 
CTCATGTGGC ACATCTCATC CCAAGTTGCA GAACTGAAGA AACTTGTGGT GGACAATGTG 
GAGGTTTTAA CCCAGATGAG GACCAGCTTT GACAAGCCGG ACCATATGGC AGCACTCTTT 
AAGAGGCTAA CATCGGTTGA CAGCGTGCTG AAACGGATGA CCATCATTGG TGTGATTTTG 
TCGTTCCGCT CTCTGGCTCA GGAAGCTCTC CGAGATGTGC TCTCTTGCCA CATTCCCTTC 
CTGGTGAGCT CCGTTGAAGA CTTTAAAGAT CACATTCCTC GTGAAACTGA CATGAAGGTG 
GCCATGAACG TGTACGAGCT GTCTTCTGCT GCAGGCCTGC CCTGTGAGAT CGACCCTGCT 
CTAGTAGTGG CGCTGTCATC CCAGAAGTCT GAAAATATTA GCCCGGAGGA AGAGTACAAG 
ATCGCCTGCC TGTTGATGGT CTTCGTGGCC GTTTCCATGC CGACCCTGGC CAGCAACGTG 
ATGTCACAGT ACAGTCCCGC AATAGAAGGT CACTGCAACA ACATTCACTG TCTGGCTAAA 
GCGATAAACC AGATTGCCGC CGCTCTGTTC ACGATCCACA AGGGCAGCAT AGAGGATCGC 
CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC AGCCTGCTGA AGATTGGACA GGAGACGGAT 
AAAACCACTA CACGGAACAG AGAGTCTGTT TACCTGCTCC TGGACATGAT CGTTCAAGAA 
TCGCCATTCC TCACCATGGA CCTGTTGGAG TCCTGCTTTC CATACGTCCT GCTCCGAAAC 
GCCTATCACG CCGTATACAA GCAGTCGGTA TCTTCTTCAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167978.1
CDS	212..3616
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	XM_005167921.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167921.4

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT 
TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG 
TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAAAA AGAAAAGTCA GAAATACTGA AGAATTTAGC TCTATATTAC 
TTCACCTTTG TGGACGTTAT GGAATTTAAG GACCACGTTT GTGAACTGCT GAACACTATT 
GATGCTTGTC AAGTTTTCTT TGACATTACT GTAAATTTTG ACCTCACAAA GAACTACCTG 
GACTTGGTAG TGACGTACAC TACCCTGATG TTGCTGCTTT CCCGAATTGA AGAGCGCAAA 
GCGATAATCG GCCTCTACAA CTATGCCCAT GAAATGACAC ACGGTTCAAG CGATCGAGAA 
TACCCCAGGC TTGGACAGAT GATTGTGGAT TACGAGAACC CACTAAAAAA GATGATGGAG 
GAGTTTGTCC CTCATGGAAA ATCGCTGTCA GATGCGCTGG TCTCCTTGCA GATGGTGTAC 
CCACGCCGGA ATCTGTCTGC AGATCAGTGG AGAAACGCTC AGCTGCTCAG TTTGATTAGC 
GCTCCCAGCA CTATGCTCAA CCCAGCTCAA TCTGACACGA TGCCCTGTGA ATACCTATCC 
TTGGACACCA TGGAGAAATG GATCGTCTTC GGCTTCATCT TGTGTCATGC GGCTCTGAAC 
AGTGATCCTG CGGCTCTCAG TCTTTGGAAG TTGGCCCTTC AGAGCAGTAC CTGTCTCTGT 
CTGTTCAGGG ACGAGGTCTT CCACATTCAC AAAGCTGCAG AGGATCTCTT CGTAAACATT 
CGCGGTTACA ACAAACGTGT CAATGATATT CGTGAATGCA AAGAATCTGC TCTTTCACAT 
GCGGGCTCAA TGCACAGAGA ACGACGTAAG TTCTTGAGGT CTGCCCTGAA AGAGCTAGCC 
ACAGTACTGG CAGATCAGCC TGGATTGCTG GGTCCGAAGG CTCTGTTTGT GTTCATGGCA 
CTCTCCTTCG CCCGTGATGA GATCATTTGG CTGCTCAGAC ATGCCGACAA CATCCAAAAG 
AAAAGCACAG ATGACTTCAT TGACAAGCAC ATTGCAGAGT TGATCTTCTA CATGGAGGAG 
TTGAGGGCTC ACGTCCGAAA GTACGGGCCG GTGATGCAGA GGTACTATGT TCAGTACCTG 
TCCGGCTTTG ACGCGGTGGT GCTCAATGAG CTTGTACAGA ACCTGTCCGT GTGCCCAGAG 
GACGAGTCCA TCATAATGTC GTCCTTCGTG AACACTATGA CCTCTCTCAG TGTTAAACAA 
GTTGAAGATG GCGAAGTGTT TGATTTCAGA GGGATGAGGC TGGACTGGTT CAGACTTCAG 
GCCTACACCA GCGTGTCCAA GGCTTCGTTG GGACTGGCAG ATCACAGAGA ACTGGGAAAG 
ATGATGAACA CCATCATCTT CCACACTAAG ATGGTGGACT CTCTGGTGGA GATGCTTGTG 
GAGACTTCTG ACCTGTCCAT ATTCTGTTTT TACAGCCGCG CCTTTGAAAA GATGTTCCAG 
CAGTGTCTTG AACTGCCGTC CCAGTCTCGA TACTCCATCT CCTTCCCACT GCTCTGCACC 
CACTTTATGA GCTGCACTCA TGAGCTCTGC CCTGAAGAGA GGCACCACAT CGGAGACCGC 
AGCCTGTCCC TTTGCAACAT GTTCCTGGAC GAGATGGCCA AGCAAGCCCG CAACCTCATC 
ACAGACATAT GCACAGAGCA GTGCACCCTG AGCGACCAGC TACTGCCAAA ACATTGTGCC 
AAAACCATCA GTCAGGCCGT GAACAAGAAG TCGAAAAAGC AGACAGGGAA GAAGGGTGAA 
CCGGAGCGAG AAAAACCTGG CGTGGAGAGC ATGAGGAAGA ACAGACTCCT GGTCACCAAT 
CTGGATAAAC TGCACACTGC TCTGTCCGAG CTCTGCTTCT CCATCAACTA CGTCCCCAAC 
ATGATGGTCT GGGAGCACAC CTTCACCCCA CGCGAGTACC TGACCTCCCA CCTGGAGATT 
CGCTTCACTA AGTCTATAGT CGGGATGACC ATGTACAACC AGACAACACA GGAGATCGCC 
AAACCATCAG AGCTGCTCAC CAGCGTCCGG GCCTACATGA CAGTGCTGCA GTCCATTGAG 
AATTATGTGC AGATTGACAT CACCAGAGTC TTCAACAATG TGCTGCTGCA GCAAACCCAA 
CACCTGGACA GCCACGGAGA GCCAACCATC ACCAGCCTCT ACACTAACTG GTATCTGGAG 
ACTTTACTGC GGCAGGTCAG CAACGGTCAC ATCGCGTACT TTCCTGCGAT GAAAGCCTTC 
GTCAACTTGC CCACGGAGAA TGAGCTGACC TTCAACGCAG AGGAGTATTC TGACATTTCT 
GAAATGCGCT CTCTGTCGGA GCTGCTGGGT CCATATGGGA TGAAGTTCCT CAGCGAGAGT 
CTCATGTGGC ACATCTCATC CCAAGTTGCA GAACTGAAGA AACTTGTGGT GGACAATGTG 
GAGGTTTTAA CCCAGATGAG GACCAGCTTT GACAAGCCGG ACCATATGGC AGCACTCTTT 
AAGAGGCTAA CATCGGTTGA CAGCGTGCTG AAACGGATGA CCATCATTGG TGTGATTTTG 
TCGTTCCGCT CTCTGGCTCA GGAAGCTCTC CGAGATGTGC TCTCTTGCCA CATTCCCTTC 
CTGGTGAGCT CCGTTGAAGA CTTTAAAGAT CACATTCCTC GTGAAACTGA CATGAAGGTG 
GCCATGAACG TGTACGAGCT GTCTTCTGCT GCAGGCCTGC CCTGTGAGAT CGACCCTGCT 
CTAGTAGTGG CGCTGTCATC CCAGAAGTCT GAAAATATTA GCCCGGAGGA AGAGTACAAG 
ATCGCCTGCC TGTTGATGGT CTTCGTGGCC GTTTCCATGC CGACCCTGGC CAGCAACGTG 
ATGTCACAGT ACAGTCCCGC AATAGAAGGT CACTGCAACA ACATTCACTG TCTGGCTAAA 
GCGATAAACC AGATTGCCGC CGCTCTGTTC ACGATCCACA AGGGCAGCAT AGAGGATCGC 
CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC AGCCTGCTGA AGATTGGACA GGAGACGGAT 
AAAACCACTA CACGGAACAG AGAGTCTGTT TACCTGCTCC TGGACATGAT CGTTCAAGAA 
TCGCCATTCC TCACCATGGA CCTGTTGGAG TCCTGCTTTC CATACGTCCT GCTCCGAAAC 
GCCTATCACG CCGTATACAA GCAGTCGGTA TCTTCTTCAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_005167921.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167978.1 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

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CloneID	ODa15520
Clone ID Related Accession (Same CDS sequence)	XM_005167921.4 , XM_005167921.3
Accession Version	XM_005167921.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nck-associated protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT 
TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG 
TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAAAA AGAAAAGTCA GAAATACTGA AGAATTTAGC TCTATATTAC 
TTCACCTTTG TGGACGTTAT GGAATTTAAG GACCACGTTT GTGAACTGCT GAACACTATT 
GATGCTTGTC AAGTTTTCTT TGACATTACT GTAAATTTTG ACCTCACAAA GAACTACCTG 
GACTTGGTAG TGACGTACAC TACCCTGATG TTGCTGCTTT CCCGAATTGA AGAGCGCAAA 
GCGATAATCG GCCTCTACAA CTATGCCCAT GAAATGACAC ACGGTTCAAG CGATCGAGAA 
TACCCCAGGC TTGGACAGAT GATTGTGGAT TACGAGAACC CACTAAAAAA GATGATGGAG 
GAGTTTGTCC CTCATGGAAA ATCGCTGTCA GATGCGCTGG TCTCCTTGCA GATGGTGTAC 
CCACGCCGGA ATCTGTCTGC AGATCAGTGG AGAAACGCTC AGCTGCTCAG TTTGATTAGC 
GCTCCCAGCA CTATGCTCAA CCCAGCTCAA TCTGACACGA TGCCCTGTGA ATACCTATCC 
TTGGACACCA TGGAGAAATG GATCGTCTTC GGCTTCATCT TGTGTCATGC GGCTCTGAAC 
AGTGATCCTG CGGCTCTCAG TCTTTGGAAG TTGGCCCTTC AGAGCAGTAC CTGTCTCTGT 
CTGTTCAGGG ACGAGGTCTT CCACATTCAC AAAGCTGCAG AGGATCTCTT CGTAAACATT 
CGCGGTTACA ACAAACGTGT CAATGATATT CGTGAATGCA AAGAATCTGC TCTTTCACAT 
GCGGGCTCAA TGCACAGAGA ACGACGTAAG TTCTTGAGGT CTGCCCTGAA AGAGCTAGCC 
ACAGTACTGG CAGATCAGCC TGGATTGCTG GGTCCGAAGG CTCTGTTTGT GTTCATGGCA 
CTCTCCTTCG CCCGTGATGA GATCATTTGG CTGCTCAGAC ATGCCGACAA CATCCAAAAG 
AAAAGCACAG ATGACTTCAT TGACAAGCAC ATTGCAGAGT TGATCTTCTA CATGGAGGAG 
TTGAGGGCTC ACGTCCGAAA GTACGGGCCG GTGATGCAGA GGTACTATGT TCAGTACCTG 
TCCGGCTTTG ACGCGGTGGT GCTCAATGAG CTTGTACAGA ACCTGTCCGT GTGCCCAGAG 
GACGAGTCCA TCATAATGTC GTCCTTCGTG AACACTATGA CCTCTCTCAG TGTTAAACAA 
GTTGAAGATG GCGAAGTGTT TGATTTCAGA GGGATGAGGC TGGACTGGTT CAGACTTCAG 
GCCTACACCA GCGTGTCCAA GGCTTCGTTG GGACTGGCAG ATCACAGAGA ACTGGGAAAG 
ATGATGAACA CCATCATCTT CCACACTAAG ATGGTGGACT CTCTGGTGGA GATGCTTGTG 
GAGACTTCTG ACCTGTCCAT ATTCTGTTTT TACAGCCGCG CCTTTGAAAA GATGTTCCAG 
CAGTGTCTTG AACTGCCGTC CCAGTCTCGA TACTCCATCT CCTTCCCACT GCTCTGCACC 
CACTTTATGA GCTGCACTCA TGAGCTCTGC CCTGAAGAGA GGCACCACAT CGGAGACCGC 
AGCCTGTCCC TTTGCAACAT GTTCCTGGAC GAGATGGCCA AGCAAGCCCG CAACCTCATC 
ACAGACATAT GCACAGAGCA GTGCACCCTG AGCGACCAGC TACTGCCAAA ACATTGTGCC 
AAAACCATCA GTCAGGCCGT GAACAAGAAG TCGAAAAAGC AGACAGGGAA GAAGGGTGAA 
CCGGAGCGAG AAAAACCTGG CGTGGAGAGC ATGAGGAAGA ACAGACTCCT GGTCACCAAT 
CTGGATAAAC TGCACACTGC TCTGTCCGAG CTCTGCTTCT CCATCAACTA CGTCCCCAAC 
ATGATGGTCT GGGAGCACAC CTTCACCCCA CGCGAGTACC TGACCTCCCA CCTGGAGATT 
CGCTTCACTA AGTCTATAGT CGGGATGACC ATGTACAACC AGACAACACA GGAGATCGCC 
AAACCATCAG AGCTGCTCAC CAGCGTCCGG GCCTACATGA CAGTGCTGCA GTCCATTGAG 
AATTATGTGC AGATTGACAT CACCAGAGTC TTCAACAATG TGCTGCTGCA GCAAACCCAA 
CACCTGGACA GCCACGGAGA GCCAACCATC ACCAGCCTCT ACACTAACTG GTATCTGGAG 
ACTTTACTGC GGCAGGTCAG CAACGGTCAC ATCGCGTACT TTCCTGCGAT GAAAGCCTTC 
GTCAACTTGC CCACGGAGAA TGAGCTGACC TTCAACGCAG AGGAGTATTC TGACATTTCT 
GAAATGCGCT CTCTGTCGGA GCTGCTGGGT CCATATGGGA TGAAGTTCCT CAGCGAGAGT 
CTCATGTGGC ACATCTCATC CCAAGTTGCA GAACTGAAGA AACTTGTGGT GGACAATGTG 
GAGGTTTTAA CCCAGATGAG GACCAGCTTT GACAAGCCGG ACCATATGGC AGCACTCTTT 
AAGAGGCTAA CATCGGTTGA CAGCGTGCTG AAACGGATGA CCATCATTGG TGTGATTTTG 
TCGTTCCGCT CTCTGGCTCA GGAAGCTCTC CGAGATGTGC TCTCTTGCCA CATTCCCTTC 
CTGGTGAGCT CCGTTGAAGA CTTTAAAGAT CACATTCCTC GTGAAACTGA CATGAAGGTG 
GCCATGAACG TGTACGAGCT GTCTTCTGCT GCAGGCCTGC CCTGTGAGAT CGACCCTGCT 
CTAGTAGTGG CGCTGTCATC CCAGAAGTCT GAAAATATTA GCCCGGAGGA AGAGTACAAG 
ATCGCCTGCC TGTTGATGGT CTTCGTGGCC GTTTCCATGC CGACCCTGGC CAGCAACGTG 
ATGTCACAGT ACAGTCCCGC AATAGAAGGT CACTGCAACA ACATTCACTG TCTGGCTAAA 
GCGATAAACC AGATTGCCGC CGCTCTGTTC ACGATCCACA AGGGCAGCAT AGAGGATCGC 
CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC AGCCTGCTGA AGATTGGACA GGAGACGGAT 
AAAACCACTA CACGGAACAG AGAGTCTGTT TACCTGCTCC TGGACATGAT CGTTCAAGAA 
TCGCCATTCC TCACCATGGA CCTGTTGGAG TCCTGCTTTC CATACGTCCT GCTCCGAAAC 
GCCTATCACG CCGTATACAA GCAGTCGGTA TCTTCTTCAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167978.1
CDS	1..3405
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	XM_005167921.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167921.3

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT 
TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG 
TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAAAA AGAAAAGTCA GAAATACTGA AGAATTTAGC TCTATATTAC 
TTCACCTTTG TGGACGTTAT GGAATTTAAG GACCACGTTT GTGAACTGCT GAACACTATT 
GATGCTTGTC AAGTTTTCTT TGACATTACT GTAAATTTTG ACCTCACAAA GAACTACCTG 
GACTTGGTAG TGACGTACAC TACCCTGATG TTGCTGCTTT CCCGAATTGA AGAGCGCAAA 
GCGATAATCG GCCTCTACAA CTATGCCCAT GAAATGACAC ACGGTTCAAG CGATCGAGAA 
TACCCCAGGC TTGGACAGAT GATTGTGGAT TACGAGAACC CACTAAAAAA GATGATGGAG 
GAGTTTGTCC CTCATGGAAA ATCGCTGTCA GATGCGCTGG TCTCCTTGCA GATGGTGTAC 
CCACGCCGGA ATCTGTCTGC AGATCAGTGG AGAAACGCTC AGCTGCTCAG TTTGATTAGC 
GCTCCCAGCA CTATGCTCAA CCCAGCTCAA TCTGACACGA TGCCCTGTGA ATACCTATCC 
TTGGACACCA TGGAGAAATG GATCGTCTTC GGCTTCATCT TGTGTCATGC GGCTCTGAAC 
AGTGATCCTG CGGCTCTCAG TCTTTGGAAG TTGGCCCTTC AGAGCAGTAC CTGTCTCTGT 
CTGTTCAGGG ACGAGGTCTT CCACATTCAC AAAGCTGCAG AGGATCTCTT CGTAAACATT 
CGCGGTTACA ACAAACGTGT CAATGATATT CGTGAATGCA AAGAATCTGC TCTTTCACAT 
GCGGGCTCAA TGCACAGAGA ACGACGTAAG TTCTTGAGGT CTGCCCTGAA AGAGCTAGCC 
ACAGTACTGG CAGATCAGCC TGGATTGCTG GGTCCGAAGG CTCTGTTTGT GTTCATGGCA 
CTCTCCTTCG CCCGTGATGA GATCATTTGG CTGCTCAGAC ATGCCGACAA CATCCAAAAG 
AAAAGCACAG ATGACTTCAT TGACAAGCAC ATTGCAGAGT TGATCTTCTA CATGGAGGAG 
TTGAGGGCTC ACGTCCGAAA GTACGGGCCG GTGATGCAGA GGTACTATGT TCAGTACCTG 
TCCGGCTTTG ACGCGGTGGT GCTCAATGAG CTTGTACAGA ACCTGTCCGT GTGCCCAGAG 
GACGAGTCCA TCATAATGTC GTCCTTCGTG AACACTATGA CCTCTCTCAG TGTTAAACAA 
GTTGAAGATG GCGAAGTGTT TGATTTCAGA GGGATGAGGC TGGACTGGTT CAGACTTCAG 
GCCTACACCA GCGTGTCCAA GGCTTCGTTG GGACTGGCAG ATCACAGAGA ACTGGGAAAG 
ATGATGAACA CCATCATCTT CCACACTAAG ATGGTGGACT CTCTGGTGGA GATGCTTGTG 
GAGACTTCTG ACCTGTCCAT ATTCTGTTTT TACAGCCGCG CCTTTGAAAA GATGTTCCAG 
CAGTGTCTTG AACTGCCGTC CCAGTCTCGA TACTCCATCT CCTTCCCACT GCTCTGCACC 
CACTTTATGA GCTGCACTCA TGAGCTCTGC CCTGAAGAGA GGCACCACAT CGGAGACCGC 
AGCCTGTCCC TTTGCAACAT GTTCCTGGAC GAGATGGCCA AGCAAGCCCG CAACCTCATC 
ACAGACATAT GCACAGAGCA GTGCACCCTG AGCGACCAGC TACTGCCAAA ACATTGTGCC 
AAAACCATCA GTCAGGCCGT GAACAAGAAG TCGAAAAAGC AGACAGGGAA GAAGGGTGAA 
CCGGAGCGAG AAAAACCTGG CGTGGAGAGC ATGAGGAAGA ACAGACTCCT GGTCACCAAT 
CTGGATAAAC TGCACACTGC TCTGTCCGAG CTCTGCTTCT CCATCAACTA CGTCCCCAAC 
ATGATGGTCT GGGAGCACAC CTTCACCCCA CGCGAGTACC TGACCTCCCA CCTGGAGATT 
CGCTTCACTA AGTCTATAGT CGGGATGACC ATGTACAACC AGACAACACA GGAGATCGCC 
AAACCATCAG AGCTGCTCAC CAGCGTCCGG GCCTACATGA CAGTGCTGCA GTCCATTGAG 
AATTATGTGC AGATTGACAT CACCAGAGTC TTCAACAATG TGCTGCTGCA GCAAACCCAA 
CACCTGGACA GCCACGGAGA GCCAACCATC ACCAGCCTCT ACACTAACTG GTATCTGGAG 
ACTTTACTGC GGCAGGTCAG CAACGGTCAC ATCGCGTACT TTCCTGCGAT GAAAGCCTTC 
GTCAACTTGC CCACGGAGAA TGAGCTGACC TTCAACGCAG AGGAGTATTC TGACATTTCT 
GAAATGCGCT CTCTGTCGGA GCTGCTGGGT CCATATGGGA TGAAGTTCCT CAGCGAGAGT 
CTCATGTGGC ACATCTCATC CCAAGTTGCA GAACTGAAGA AACTTGTGGT GGACAATGTG 
GAGGTTTTAA CCCAGATGAG GACCAGCTTT GACAAGCCGG ACCATATGGC AGCACTCTTT 
AAGAGGCTAA CATCGGTTGA CAGCGTGCTG AAACGGATGA CCATCATTGG TGTGATTTTG 
TCGTTCCGCT CTCTGGCTCA GGAAGCTCTC CGAGATGTGC TCTCTTGCCA CATTCCCTTC 
CTGGTGAGCT CCGTTGAAGA CTTTAAAGAT CACATTCCTC GTGAAACTGA CATGAAGGTG 
GCCATGAACG TGTACGAGCT GTCTTCTGCT GCAGGCCTGC CCTGTGAGAT CGACCCTGCT 
CTAGTAGTGG CGCTGTCATC CCAGAAGTCT GAAAATATTA GCCCGGAGGA AGAGTACAAG 
ATCGCCTGCC TGTTGATGGT CTTCGTGGCC GTTTCCATGC CGACCCTGGC CAGCAACGTG 
ATGTCACAGT ACAGTCCCGC AATAGAAGGT CACTGCAACA ACATTCACTG TCTGGCTAAA 
GCGATAAACC AGATTGCCGC CGCTCTGTTC ACGATCCACA AGGGCAGCAT AGAGGATCGC 
CTCAAAGAAT TCCTCGCCCT CGCCTCCTCC AGCCTGCTGA AGATTGGACA GGAGACGGAT 
AAAACCACTA CACGGAACAG AGAGTCTGTT TACCTGCTCC TGGACATGAT CGTTCAAGAA 
TCGCCATTCC TCACCATGGA CCTGTTGGAG TCCTGCTTTC CATACGTCCT GCTCCGAAAC 
GCCTATCACG CCGTATACAA GCAGTCGGTA TCTTCTTCAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_005167922.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167979.1 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15521
Clone ID Related Accession (Same CDS sequence)	XM_005167922.4 , XM_005167922.3
Accession Version	XM_005167922.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nck-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167922.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT 
TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG 
TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCTT 
GCACAACTAC AAAAAGAAAA GTCAGAAATA CTGAAGAATT TAGCTCTATA TTACTTCACC 
TTTGTGGACG TTATGGAATT TAAGGACCAC GTTTGTGAAC TGCTGAACAC TATTGATGCT 
TGTCAAGTTT TCTTTGACAT TACTGTAAAT TTTGACCTCA CAAAGAACTA CCTGGACTTG 
GTAGTGACGT ACACTACCCT GATGTTGCTG CTTTCCCGAA TTGAAGAGCG CAAAGCGATA 
ATCGGCCTCT ACAACTATGC CCATGAAATG ACACACGGTT CAAGCGATCG AGAATACCCC 
AGGCTTGGAC AGATGATTGT GGATTACGAG AACCCACTAA AAAAGATGAT GGAGGAGTTT 
GTCCCTCATG GAAAATCGCT GTCAGATGCG CTGGTCTCCT TGCAGATGGT GTACCCACGC 
CGGAATCTGT CTGCAGATCA GTGGAGAAAC GCTCAGCTGC TCAGTTTGAT TAGCGCTCCC 
AGCACTATGC TCAACCCAGC TCAATCTGAC ACGATGCCCT GTGAATACCT ATCCTTGGAC 
ACCATGGAGA AATGGATCGT CTTCGGCTTC ATCTTGTGTC ATGCGGCTCT GAACAGTGAT 
CCTGCGGCTC TCAGTCTTTG GAAGTTGGCC CTTCAGAGCA GTACCTGTCT CTGTCTGTTC 
AGGGACGAGG TCTTCCACAT TCACAAAGCT GCAGAGGATC TCTTCGTAAA CATTCGCGGT 
TACAACAAAC GTGTCAATGA TATTCGTGAA TGCAAAGAAT CTGCTCTTTC ACATGCGGGC 
TCAATGCACA GAGAACGACG TAAGTTCTTG AGGTCTGCCC TGAAAGAGCT AGCCACAGTA 
CTGGCAGATC AGCCTGGATT GCTGGGTCCG AAGGCTCTGT TTGTGTTCAT GGCACTCTCC 
TTCGCCCGTG ATGAGATCAT TTGGCTGCTC AGACATGCCG ACAACATCCA AAAGAAAAGC 
ACAGATGACT TCATTGACAA GCACATTGCA GAGTTGATCT TCTACATGGA GGAGTTGAGG 
GCTCACGTCC GAAAGTACGG GCCGGTGATG CAGAGGTACT ATGTTCAGTA CCTGTCCGGC 
TTTGACGCGG TGGTGCTCAA TGAGCTTGTA CAGAACCTGT CCGTGTGCCC AGAGGACGAG 
TCCATCATAA TGTCGTCCTT CGTGAACACT ATGACCTCTC TCAGTGTTAA ACAAGTTGAA 
GATGGCGAAG TGTTTGATTT CAGAGGGATG AGGCTGGACT GGTTCAGACT TCAGGCCTAC 
ACCAGCGTGT CCAAGGCTTC GTTGGGACTG GCAGATCACA GAGAACTGGG AAAGATGATG 
AACACCATCA TCTTCCACAC TAAGATGGTG GACTCTCTGG TGGAGATGCT TGTGGAGACT 
TCTGACCTGT CCATATTCTG TTTTTACAGC CGCGCCTTTG AAAAGATGTT CCAGCAGTGT 
CTTGAACTGC CGTCCCAGTC TCGATACTCC ATCTCCTTCC CACTGCTCTG CACCCACTTT 
ATGAGCTGCA CTCATGAGCT CTGCCCTGAA GAGAGGCACC ACATCGGAGA CCGCAGCCTG 
TCCCTTTGCA ACATGTTCCT GGACGAGATG GCCAAGCAAG CCCGCAACCT CATCACAGAC 
ATATGCACAG AGCAGTGCAC CCTGAGCGAC CAGCTACTGC CAAAACATTG TGCCAAAACC 
ATCAGTCAGG CCGTGAACAA GAAGTCGAAA AAGCAGACAG GGAAGAAGGG TGAACCGGAG 
CGAGAAAAAC CTGGCGTGGA GAGCATGAGG AAGAACAGAC TCCTGGTCAC CAATCTGGAT 
AAACTGCACA CTGCTCTGTC CGAGCTCTGC TTCTCCATCA ACTACGTCCC CAACATGATG 
GTCTGGGAGC ACACCTTCAC CCCACGCGAG TACCTGACCT CCCACCTGGA GATTCGCTTC 
ACTAAGTCTA TAGTCGGGAT GACCATGTAC AACCAGACAA CACAGGAGAT CGCCAAACCA 
TCAGAGCTGC TCACCAGCGT CCGGGCCTAC ATGACAGTGC TGCAGTCCAT TGAGAATTAT 
GTGCAGATTG ACATCACCAG AGTCTTCAAC AATGTGCTGC TGCAGCAAAC CCAACACCTG 
GACAGCCACG GAGAGCCAAC CATCACCAGC CTCTACACTA ACTGGTATCT GGAGACTTTA 
CTGCGGCAGG TCAGCAACGG TCACATCGCG TACTTTCCTG CGATGAAAGC CTTCGTCAAC 
TTGCCCACGG AGAATGAGCT GACCTTCAAC GCAGAGGAGT ATTCTGACAT TTCTGAAATG 
CGCTCTCTGT CGGAGCTGCT GGGTCCATAT GGGATGAAGT TCCTCAGCGA GAGTCTCATG 
TGGCACATCT CATCCCAAGT TGCAGAACTG AAGAAACTTG TGGTGGACAA TGTGGAGGTT 
TTAACCCAGA TGAGGACCAG CTTTGACAAG CCGGACCATA TGGCAGCACT CTTTAAGAGG 
CTAACATCGG TTGACAGCGT GCTGAAACGG ATGACCATCA TTGGTGTGAT TTTGTCGTTC 
CGCTCTCTGG CTCAGGAAGC TCTCCGAGAT GTGCTCTCTT GCCACATTCC CTTCCTGGTG 
AGCTCCGTTG AAGACTTTAA AGATCACATT CCTCGTGAAA CTGACATGAA GGTGGCCATG 
AACGTGTACG AGCTGTCTTC TGCTGCAGGC CTGCCCTGTG AGATCGACCC TGCTCTAGTA 
GTGGCGCTGT CATCCCAGAA GTCTGAAAAT ATTAGCCCGG AGGAAGAGTA CAAGATCGCC 
TGCCTGTTGA TGGTCTTCGT GGCCGTTTCC ATGCCGACCC TGGCCAGCAA CGTGATGTCA 
CAGTACAGTC CCGCAATAGA AGGTCACTGC AACAACATTC ACTGTCTGGC TAAAGCGATA 
AACCAGATTG CCGCCGCTCT GTTCACGATC CACAAGGGCA GCATAGAGGA TCGCCTCAAA 
GAATTCCTCG CCCTCGCCTC CTCCAGCCTG CTGAAGATTG GACAGGAGAC GGATAAAACC 
ACTACACGGA ACAGAGAGTC TGTTTACCTG CTCCTGGACA TGATCGTTCA AGAATCGCCA 
TTCCTCACCA TGGACCTGTT GGAGTCCTGC TTTCCATACG TCCTGCTCCG AAACGCCTAT 
CACGCCGTAT ACAAGCAGTC GGTATCTTCT TCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167979.1
CDS	212..3610
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	XM_005167922.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167922.4

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT 
TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG 
TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCTT 
GCACAACTAC AAAAAGAAAA GTCAGAAATA CTGAAGAATT TAGCTCTATA TTACTTCACC 
TTTGTGGACG TTATGGAATT TAAGGACCAC GTTTGTGAAC TGCTGAACAC TATTGATGCT 
TGTCAAGTTT TCTTTGACAT TACTGTAAAT TTTGACCTCA CAAAGAACTA CCTGGACTTG 
GTAGTGACGT ACACTACCCT GATGTTGCTG CTTTCCCGAA TTGAAGAGCG CAAAGCGATA 
ATCGGCCTCT ACAACTATGC CCATGAAATG ACACACGGTT CAAGCGATCG AGAATACCCC 
AGGCTTGGAC AGATGATTGT GGATTACGAG AACCCACTAA AAAAGATGAT GGAGGAGTTT 
GTCCCTCATG GAAAATCGCT GTCAGATGCG CTGGTCTCCT TGCAGATGGT GTACCCACGC 
CGGAATCTGT CTGCAGATCA GTGGAGAAAC GCTCAGCTGC TCAGTTTGAT TAGCGCTCCC 
AGCACTATGC TCAACCCAGC TCAATCTGAC ACGATGCCCT GTGAATACCT ATCCTTGGAC 
ACCATGGAGA AATGGATCGT CTTCGGCTTC ATCTTGTGTC ATGCGGCTCT GAACAGTGAT 
CCTGCGGCTC TCAGTCTTTG GAAGTTGGCC CTTCAGAGCA GTACCTGTCT CTGTCTGTTC 
AGGGACGAGG TCTTCCACAT TCACAAAGCT GCAGAGGATC TCTTCGTAAA CATTCGCGGT 
TACAACAAAC GTGTCAATGA TATTCGTGAA TGCAAAGAAT CTGCTCTTTC ACATGCGGGC 
TCAATGCACA GAGAACGACG TAAGTTCTTG AGGTCTGCCC TGAAAGAGCT AGCCACAGTA 
CTGGCAGATC AGCCTGGATT GCTGGGTCCG AAGGCTCTGT TTGTGTTCAT GGCACTCTCC 
TTCGCCCGTG ATGAGATCAT TTGGCTGCTC AGACATGCCG ACAACATCCA AAAGAAAAGC 
ACAGATGACT TCATTGACAA GCACATTGCA GAGTTGATCT TCTACATGGA GGAGTTGAGG 
GCTCACGTCC GAAAGTACGG GCCGGTGATG CAGAGGTACT ATGTTCAGTA CCTGTCCGGC 
TTTGACGCGG TGGTGCTCAA TGAGCTTGTA CAGAACCTGT CCGTGTGCCC AGAGGACGAG 
TCCATCATAA TGTCGTCCTT CGTGAACACT ATGACCTCTC TCAGTGTTAA ACAAGTTGAA 
GATGGCGAAG TGTTTGATTT CAGAGGGATG AGGCTGGACT GGTTCAGACT TCAGGCCTAC 
ACCAGCGTGT CCAAGGCTTC GTTGGGACTG GCAGATCACA GAGAACTGGG AAAGATGATG 
AACACCATCA TCTTCCACAC TAAGATGGTG GACTCTCTGG TGGAGATGCT TGTGGAGACT 
TCTGACCTGT CCATATTCTG TTTTTACAGC CGCGCCTTTG AAAAGATGTT CCAGCAGTGT 
CTTGAACTGC CGTCCCAGTC TCGATACTCC ATCTCCTTCC CACTGCTCTG CACCCACTTT 
ATGAGCTGCA CTCATGAGCT CTGCCCTGAA GAGAGGCACC ACATCGGAGA CCGCAGCCTG 
TCCCTTTGCA ACATGTTCCT GGACGAGATG GCCAAGCAAG CCCGCAACCT CATCACAGAC 
ATATGCACAG AGCAGTGCAC CCTGAGCGAC CAGCTACTGC CAAAACATTG TGCCAAAACC 
ATCAGTCAGG CCGTGAACAA GAAGTCGAAA AAGCAGACAG GGAAGAAGGG TGAACCGGAG 
CGAGAAAAAC CTGGCGTGGA GAGCATGAGG AAGAACAGAC TCCTGGTCAC CAATCTGGAT 
AAACTGCACA CTGCTCTGTC CGAGCTCTGC TTCTCCATCA ACTACGTCCC CAACATGATG 
GTCTGGGAGC ACACCTTCAC CCCACGCGAG TACCTGACCT CCCACCTGGA GATTCGCTTC 
ACTAAGTCTA TAGTCGGGAT GACCATGTAC AACCAGACAA CACAGGAGAT CGCCAAACCA 
TCAGAGCTGC TCACCAGCGT CCGGGCCTAC ATGACAGTGC TGCAGTCCAT TGAGAATTAT 
GTGCAGATTG ACATCACCAG AGTCTTCAAC AATGTGCTGC TGCAGCAAAC CCAACACCTG 
GACAGCCACG GAGAGCCAAC CATCACCAGC CTCTACACTA ACTGGTATCT GGAGACTTTA 
CTGCGGCAGG TCAGCAACGG TCACATCGCG TACTTTCCTG CGATGAAAGC CTTCGTCAAC 
TTGCCCACGG AGAATGAGCT GACCTTCAAC GCAGAGGAGT ATTCTGACAT TTCTGAAATG 
CGCTCTCTGT CGGAGCTGCT GGGTCCATAT GGGATGAAGT TCCTCAGCGA GAGTCTCATG 
TGGCACATCT CATCCCAAGT TGCAGAACTG AAGAAACTTG TGGTGGACAA TGTGGAGGTT 
TTAACCCAGA TGAGGACCAG CTTTGACAAG CCGGACCATA TGGCAGCACT CTTTAAGAGG 
CTAACATCGG TTGACAGCGT GCTGAAACGG ATGACCATCA TTGGTGTGAT TTTGTCGTTC 
CGCTCTCTGG CTCAGGAAGC TCTCCGAGAT GTGCTCTCTT GCCACATTCC CTTCCTGGTG 
AGCTCCGTTG AAGACTTTAA AGATCACATT CCTCGTGAAA CTGACATGAA GGTGGCCATG 
AACGTGTACG AGCTGTCTTC TGCTGCAGGC CTGCCCTGTG AGATCGACCC TGCTCTAGTA 
GTGGCGCTGT CATCCCAGAA GTCTGAAAAT ATTAGCCCGG AGGAAGAGTA CAAGATCGCC 
TGCCTGTTGA TGGTCTTCGT GGCCGTTTCC ATGCCGACCC TGGCCAGCAA CGTGATGTCA 
CAGTACAGTC CCGCAATAGA AGGTCACTGC AACAACATTC ACTGTCTGGC TAAAGCGATA 
AACCAGATTG CCGCCGCTCT GTTCACGATC CACAAGGGCA GCATAGAGGA TCGCCTCAAA 
GAATTCCTCG CCCTCGCCTC CTCCAGCCTG CTGAAGATTG GACAGGAGAC GGATAAAACC 
ACTACACGGA ACAGAGAGTC TGTTTACCTG CTCCTGGACA TGATCGTTCA AGAATCGCCA 
TTCCTCACCA TGGACCTGTT GGAGTCCTGC TTTCCATACG TCCTGCTCCG AAACGCCTAT 
CACGCCGTAT ACAAGCAGTC GGTATCTTCT TCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_005167922.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167979.1 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

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CloneID	ODa15521
Clone ID Related Accession (Same CDS sequence)	XM_005167922.4 , XM_005167922.3
Accession Version	XM_005167922.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nck-associated protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT 
TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG 
TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCTT 
GCACAACTAC AAAAAGAAAA GTCAGAAATA CTGAAGAATT TAGCTCTATA TTACTTCACC 
TTTGTGGACG TTATGGAATT TAAGGACCAC GTTTGTGAAC TGCTGAACAC TATTGATGCT 
TGTCAAGTTT TCTTTGACAT TACTGTAAAT TTTGACCTCA CAAAGAACTA CCTGGACTTG 
GTAGTGACGT ACACTACCCT GATGTTGCTG CTTTCCCGAA TTGAAGAGCG CAAAGCGATA 
ATCGGCCTCT ACAACTATGC CCATGAAATG ACACACGGTT CAAGCGATCG AGAATACCCC 
AGGCTTGGAC AGATGATTGT GGATTACGAG AACCCACTAA AAAAGATGAT GGAGGAGTTT 
GTCCCTCATG GAAAATCGCT GTCAGATGCG CTGGTCTCCT TGCAGATGGT GTACCCACGC 
CGGAATCTGT CTGCAGATCA GTGGAGAAAC GCTCAGCTGC TCAGTTTGAT TAGCGCTCCC 
AGCACTATGC TCAACCCAGC TCAATCTGAC ACGATGCCCT GTGAATACCT ATCCTTGGAC 
ACCATGGAGA AATGGATCGT CTTCGGCTTC ATCTTGTGTC ATGCGGCTCT GAACAGTGAT 
CCTGCGGCTC TCAGTCTTTG GAAGTTGGCC CTTCAGAGCA GTACCTGTCT CTGTCTGTTC 
AGGGACGAGG TCTTCCACAT TCACAAAGCT GCAGAGGATC TCTTCGTAAA CATTCGCGGT 
TACAACAAAC GTGTCAATGA TATTCGTGAA TGCAAAGAAT CTGCTCTTTC ACATGCGGGC 
TCAATGCACA GAGAACGACG TAAGTTCTTG AGGTCTGCCC TGAAAGAGCT AGCCACAGTA 
CTGGCAGATC AGCCTGGATT GCTGGGTCCG AAGGCTCTGT TTGTGTTCAT GGCACTCTCC 
TTCGCCCGTG ATGAGATCAT TTGGCTGCTC AGACATGCCG ACAACATCCA AAAGAAAAGC 
ACAGATGACT TCATTGACAA GCACATTGCA GAGTTGATCT TCTACATGGA GGAGTTGAGG 
GCTCACGTCC GAAAGTACGG GCCGGTGATG CAGAGGTACT ATGTTCAGTA CCTGTCCGGC 
TTTGACGCGG TGGTGCTCAA TGAGCTTGTA CAGAACCTGT CCGTGTGCCC AGAGGACGAG 
TCCATCATAA TGTCGTCCTT CGTGAACACT ATGACCTCTC TCAGTGTTAA ACAAGTTGAA 
GATGGCGAAG TGTTTGATTT CAGAGGGATG AGGCTGGACT GGTTCAGACT TCAGGCCTAC 
ACCAGCGTGT CCAAGGCTTC GTTGGGACTG GCAGATCACA GAGAACTGGG AAAGATGATG 
AACACCATCA TCTTCCACAC TAAGATGGTG GACTCTCTGG TGGAGATGCT TGTGGAGACT 
TCTGACCTGT CCATATTCTG TTTTTACAGC CGCGCCTTTG AAAAGATGTT CCAGCAGTGT 
CTTGAACTGC CGTCCCAGTC TCGATACTCC ATCTCCTTCC CACTGCTCTG CACCCACTTT 
ATGAGCTGCA CTCATGAGCT CTGCCCTGAA GAGAGGCACC ACATCGGAGA CCGCAGCCTG 
TCCCTTTGCA ACATGTTCCT GGACGAGATG GCCAAGCAAG CCCGCAACCT CATCACAGAC 
ATATGCACAG AGCAGTGCAC CCTGAGCGAC CAGCTACTGC CAAAACATTG TGCCAAAACC 
ATCAGTCAGG CCGTGAACAA GAAGTCGAAA AAGCAGACAG GGAAGAAGGG TGAACCGGAG 
CGAGAAAAAC CTGGCGTGGA GAGCATGAGG AAGAACAGAC TCCTGGTCAC CAATCTGGAT 
AAACTGCACA CTGCTCTGTC CGAGCTCTGC TTCTCCATCA ACTACGTCCC CAACATGATG 
GTCTGGGAGC ACACCTTCAC CCCACGCGAG TACCTGACCT CCCACCTGGA GATTCGCTTC 
ACTAAGTCTA TAGTCGGGAT GACCATGTAC AACCAGACAA CACAGGAGAT CGCCAAACCA 
TCAGAGCTGC TCACCAGCGT CCGGGCCTAC ATGACAGTGC TGCAGTCCAT TGAGAATTAT 
GTGCAGATTG ACATCACCAG AGTCTTCAAC AATGTGCTGC TGCAGCAAAC CCAACACCTG 
GACAGCCACG GAGAGCCAAC CATCACCAGC CTCTACACTA ACTGGTATCT GGAGACTTTA 
CTGCGGCAGG TCAGCAACGG TCACATCGCG TACTTTCCTG CGATGAAAGC CTTCGTCAAC 
TTGCCCACGG AGAATGAGCT GACCTTCAAC GCAGAGGAGT ATTCTGACAT TTCTGAAATG 
CGCTCTCTGT CGGAGCTGCT GGGTCCATAT GGGATGAAGT TCCTCAGCGA GAGTCTCATG 
TGGCACATCT CATCCCAAGT TGCAGAACTG AAGAAACTTG TGGTGGACAA TGTGGAGGTT 
TTAACCCAGA TGAGGACCAG CTTTGACAAG CCGGACCATA TGGCAGCACT CTTTAAGAGG 
CTAACATCGG TTGACAGCGT GCTGAAACGG ATGACCATCA TTGGTGTGAT TTTGTCGTTC 
CGCTCTCTGG CTCAGGAAGC TCTCCGAGAT GTGCTCTCTT GCCACATTCC CTTCCTGGTG 
AGCTCCGTTG AAGACTTTAA AGATCACATT CCTCGTGAAA CTGACATGAA GGTGGCCATG 
AACGTGTACG AGCTGTCTTC TGCTGCAGGC CTGCCCTGTG AGATCGACCC TGCTCTAGTA 
GTGGCGCTGT CATCCCAGAA GTCTGAAAAT ATTAGCCCGG AGGAAGAGTA CAAGATCGCC 
TGCCTGTTGA TGGTCTTCGT GGCCGTTTCC ATGCCGACCC TGGCCAGCAA CGTGATGTCA 
CAGTACAGTC CCGCAATAGA AGGTCACTGC AACAACATTC ACTGTCTGGC TAAAGCGATA 
AACCAGATTG CCGCCGCTCT GTTCACGATC CACAAGGGCA GCATAGAGGA TCGCCTCAAA 
GAATTCCTCG CCCTCGCCTC CTCCAGCCTG CTGAAGATTG GACAGGAGAC GGATAAAACC 
ACTACACGGA ACAGAGAGTC TGTTTACCTG CTCCTGGACA TGATCGTTCA AGAATCGCCA 
TTCCTCACCA TGGACCTGTT GGAGTCCTGC TTTCCATACG TCCTGCTCCG AAACGCCTAT 
CACGCCGTAT ACAAGCAGTC GGTATCTTCT TCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167979.1
CDS	230..3628
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	XM_005167922.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167922.3

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGCA AGGACAAGTT 
TGGAAGGCCT GTGGAGATCC CAAGGCAAAG CCTTCCTACC TGATTGACAA AAACCTGGAG 
TCAGCCGTGA AATTCATTGT CAGAAAATTC CCAGCCGTTG AGACCCGCAA CAACAATCTT 
GCACAACTAC AAAAAGAAAA GTCAGAAATA CTGAAGAATT TAGCTCTATA TTACTTCACC 
TTTGTGGACG TTATGGAATT TAAGGACCAC GTTTGTGAAC TGCTGAACAC TATTGATGCT 
TGTCAAGTTT TCTTTGACAT TACTGTAAAT TTTGACCTCA CAAAGAACTA CCTGGACTTG 
GTAGTGACGT ACACTACCCT GATGTTGCTG CTTTCCCGAA TTGAAGAGCG CAAAGCGATA 
ATCGGCCTCT ACAACTATGC CCATGAAATG ACACACGGTT CAAGCGATCG AGAATACCCC 
AGGCTTGGAC AGATGATTGT GGATTACGAG AACCCACTAA AAAAGATGAT GGAGGAGTTT 
GTCCCTCATG GAAAATCGCT GTCAGATGCG CTGGTCTCCT TGCAGATGGT GTACCCACGC 
CGGAATCTGT CTGCAGATCA GTGGAGAAAC GCTCAGCTGC TCAGTTTGAT TAGCGCTCCC 
AGCACTATGC TCAACCCAGC TCAATCTGAC ACGATGCCCT GTGAATACCT ATCCTTGGAC 
ACCATGGAGA AATGGATCGT CTTCGGCTTC ATCTTGTGTC ATGCGGCTCT GAACAGTGAT 
CCTGCGGCTC TCAGTCTTTG GAAGTTGGCC CTTCAGAGCA GTACCTGTCT CTGTCTGTTC 
AGGGACGAGG TCTTCCACAT TCACAAAGCT GCAGAGGATC TCTTCGTAAA CATTCGCGGT 
TACAACAAAC GTGTCAATGA TATTCGTGAA TGCAAAGAAT CTGCTCTTTC ACATGCGGGC 
TCAATGCACA GAGAACGACG TAAGTTCTTG AGGTCTGCCC TGAAAGAGCT AGCCACAGTA 
CTGGCAGATC AGCCTGGATT GCTGGGTCCG AAGGCTCTGT TTGTGTTCAT GGCACTCTCC 
TTCGCCCGTG ATGAGATCAT TTGGCTGCTC AGACATGCCG ACAACATCCA AAAGAAAAGC 
ACAGATGACT TCATTGACAA GCACATTGCA GAGTTGATCT TCTACATGGA GGAGTTGAGG 
GCTCACGTCC GAAAGTACGG GCCGGTGATG CAGAGGTACT ATGTTCAGTA CCTGTCCGGC 
TTTGACGCGG TGGTGCTCAA TGAGCTTGTA CAGAACCTGT CCGTGTGCCC AGAGGACGAG 
TCCATCATAA TGTCGTCCTT CGTGAACACT ATGACCTCTC TCAGTGTTAA ACAAGTTGAA 
GATGGCGAAG TGTTTGATTT CAGAGGGATG AGGCTGGACT GGTTCAGACT TCAGGCCTAC 
ACCAGCGTGT CCAAGGCTTC GTTGGGACTG GCAGATCACA GAGAACTGGG AAAGATGATG 
AACACCATCA TCTTCCACAC TAAGATGGTG GACTCTCTGG TGGAGATGCT TGTGGAGACT 
TCTGACCTGT CCATATTCTG TTTTTACAGC CGCGCCTTTG AAAAGATGTT CCAGCAGTGT 
CTTGAACTGC CGTCCCAGTC TCGATACTCC ATCTCCTTCC CACTGCTCTG CACCCACTTT 
ATGAGCTGCA CTCATGAGCT CTGCCCTGAA GAGAGGCACC ACATCGGAGA CCGCAGCCTG 
TCCCTTTGCA ACATGTTCCT GGACGAGATG GCCAAGCAAG CCCGCAACCT CATCACAGAC 
ATATGCACAG AGCAGTGCAC CCTGAGCGAC CAGCTACTGC CAAAACATTG TGCCAAAACC 
ATCAGTCAGG CCGTGAACAA GAAGTCGAAA AAGCAGACAG GGAAGAAGGG TGAACCGGAG 
CGAGAAAAAC CTGGCGTGGA GAGCATGAGG AAGAACAGAC TCCTGGTCAC CAATCTGGAT 
AAACTGCACA CTGCTCTGTC CGAGCTCTGC TTCTCCATCA ACTACGTCCC CAACATGATG 
GTCTGGGAGC ACACCTTCAC CCCACGCGAG TACCTGACCT CCCACCTGGA GATTCGCTTC 
ACTAAGTCTA TAGTCGGGAT GACCATGTAC AACCAGACAA CACAGGAGAT CGCCAAACCA 
TCAGAGCTGC TCACCAGCGT CCGGGCCTAC ATGACAGTGC TGCAGTCCAT TGAGAATTAT 
GTGCAGATTG ACATCACCAG AGTCTTCAAC AATGTGCTGC TGCAGCAAAC CCAACACCTG 
GACAGCCACG GAGAGCCAAC CATCACCAGC CTCTACACTA ACTGGTATCT GGAGACTTTA 
CTGCGGCAGG TCAGCAACGG TCACATCGCG TACTTTCCTG CGATGAAAGC CTTCGTCAAC 
TTGCCCACGG AGAATGAGCT GACCTTCAAC GCAGAGGAGT ATTCTGACAT TTCTGAAATG 
CGCTCTCTGT CGGAGCTGCT GGGTCCATAT GGGATGAAGT TCCTCAGCGA GAGTCTCATG 
TGGCACATCT CATCCCAAGT TGCAGAACTG AAGAAACTTG TGGTGGACAA TGTGGAGGTT 
TTAACCCAGA TGAGGACCAG CTTTGACAAG CCGGACCATA TGGCAGCACT CTTTAAGAGG 
CTAACATCGG TTGACAGCGT GCTGAAACGG ATGACCATCA TTGGTGTGAT TTTGTCGTTC 
CGCTCTCTGG CTCAGGAAGC TCTCCGAGAT GTGCTCTCTT GCCACATTCC CTTCCTGGTG 
AGCTCCGTTG AAGACTTTAA AGATCACATT CCTCGTGAAA CTGACATGAA GGTGGCCATG 
AACGTGTACG AGCTGTCTTC TGCTGCAGGC CTGCCCTGTG AGATCGACCC TGCTCTAGTA 
GTGGCGCTGT CATCCCAGAA GTCTGAAAAT ATTAGCCCGG AGGAAGAGTA CAAGATCGCC 
TGCCTGTTGA TGGTCTTCGT GGCCGTTTCC ATGCCGACCC TGGCCAGCAA CGTGATGTCA 
CAGTACAGTC CCGCAATAGA AGGTCACTGC AACAACATTC ACTGTCTGGC TAAAGCGATA 
AACCAGATTG CCGCCGCTCT GTTCACGATC CACAAGGGCA GCATAGAGGA TCGCCTCAAA 
GAATTCCTCG CCCTCGCCTC CTCCAGCCTG CTGAAGATTG GACAGGAGAC GGATAAAACC 
ACTACACGGA ACAGAGAGTC TGTTTACCTG CTCCTGGACA TGATCGTTCA AGAATCGCCA 
TTCCTCACCA TGGACCTGTT GGAGTCCTGC TTTCCATACG TCCTGCTCCG AAACGCCTAT 
CACGCCGTAT ACAAGCAGTC GGTATCTTCT TCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_005167923.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167980.1 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa15522
Clone ID Related Accession (Same CDS sequence)	XM_005167923.4 , XM_005167923.3
Accession Version	XM_005167923.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nck-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167923.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC AACAGCTTGC ACAACTACAA 
AAAGAAAAGT CAGAAATACT GAAGAATTTA GCTCTATATT ACTTCACCTT TGTGGACGTT 
ATGGAATTTA AGGACCACGT TTGTGAACTG CTGAACACTA TTGATGCTTG TCAAGTTTTC 
TTTGACATTA CTGTAAATTT TGACCTCACA AAGAACTACC TGGACTTGGT AGTGACGTAC 
ACTACCCTGA TGTTGCTGCT TTCCCGAATT GAAGAGCGCA AAGCGATAAT CGGCCTCTAC 
AACTATGCCC ATGAAATGAC ACACGGTTCA AGCGATCGAG AATACCCCAG GCTTGGACAG 
ATGATTGTGG ATTACGAGAA CCCACTAAAA AAGATGATGG AGGAGTTTGT CCCTCATGGA 
AAATCGCTGT CAGATGCGCT GGTCTCCTTG CAGATGGTGT ACCCACGCCG GAATCTGTCT 
GCAGATCAGT GGAGAAACGC TCAGCTGCTC AGTTTGATTA GCGCTCCCAG CACTATGCTC 
AACCCAGCTC AATCTGACAC GATGCCCTGT GAATACCTAT CCTTGGACAC CATGGAGAAA 
TGGATCGTCT TCGGCTTCAT CTTGTGTCAT GCGGCTCTGA ACAGTGATCC TGCGGCTCTC 
AGTCTTTGGA AGTTGGCCCT TCAGAGCAGT ACCTGTCTCT GTCTGTTCAG GGACGAGGTC 
TTCCACATTC ACAAAGCTGC AGAGGATCTC TTCGTAAACA TTCGCGGTTA CAACAAACGT 
GTCAATGATA TTCGTGAATG CAAAGAATCT GCTCTTTCAC ATGCGGGCTC AATGCACAGA 
GAACGACGTA AGTTCTTGAG GTCTGCCCTG AAAGAGCTAG CCACAGTACT GGCAGATCAG 
CCTGGATTGC TGGGTCCGAA GGCTCTGTTT GTGTTCATGG CACTCTCCTT CGCCCGTGAT 
GAGATCATTT GGCTGCTCAG ACATGCCGAC AACATCCAAA AGAAAAGCAC AGATGACTTC 
ATTGACAAGC ACATTGCAGA GTTGATCTTC TACATGGAGG AGTTGAGGGC TCACGTCCGA 
AAGTACGGGC CGGTGATGCA GAGGTACTAT GTTCAGTACC TGTCCGGCTT TGACGCGGTG 
GTGCTCAATG AGCTTGTACA GAACCTGTCC GTGTGCCCAG AGGACGAGTC CATCATAATG 
TCGTCCTTCG TGAACACTAT GACCTCTCTC AGTGTTAAAC AAGTTGAAGA TGGCGAAGTG 
TTTGATTTCA GAGGGATGAG GCTGGACTGG TTCAGACTTC AGGCCTACAC CAGCGTGTCC 
AAGGCTTCGT TGGGACTGGC AGATCACAGA GAACTGGGAA AGATGATGAA CACCATCATC 
TTCCACACTA AGATGGTGGA CTCTCTGGTG GAGATGCTTG TGGAGACTTC TGACCTGTCC 
ATATTCTGTT TTTACAGCCG CGCCTTTGAA AAGATGTTCC AGCAGTGTCT TGAACTGCCG 
TCCCAGTCTC GATACTCCAT CTCCTTCCCA CTGCTCTGCA CCCACTTTAT GAGCTGCACT 
CATGAGCTCT GCCCTGAAGA GAGGCACCAC ATCGGAGACC GCAGCCTGTC CCTTTGCAAC 
ATGTTCCTGG ACGAGATGGC CAAGCAAGCC CGCAACCTCA TCACAGACAT ATGCACAGAG 
CAGTGCACCC TGAGCGACCA GCTACTGCCA AAACATTGTG CCAAAACCAT CAGTCAGGCC 
GTGAACAAGA AGTCGAAAAA GCAGACAGGG AAGAAGGGTG AACCGGAGCG AGAAAAACCT 
GGCGTGGAGA GCATGAGGAA GAACAGACTC CTGGTCACCA ATCTGGATAA ACTGCACACT 
GCTCTGTCCG AGCTCTGCTT CTCCATCAAC TACGTCCCCA ACATGATGGT CTGGGAGCAC 
ACCTTCACCC CACGCGAGTA CCTGACCTCC CACCTGGAGA TTCGCTTCAC TAAGTCTATA 
GTCGGGATGA CCATGTACAA CCAGACAACA CAGGAGATCG CCAAACCATC AGAGCTGCTC 
ACCAGCGTCC GGGCCTACAT GACAGTGCTG CAGTCCATTG AGAATTATGT GCAGATTGAC 
ATCACCAGAG TCTTCAACAA TGTGCTGCTG CAGCAAACCC AACACCTGGA CAGCCACGGA 
GAGCCAACCA TCACCAGCCT CTACACTAAC TGGTATCTGG AGACTTTACT GCGGCAGGTC 
AGCAACGGTC ACATCGCGTA CTTTCCTGCG ATGAAAGCCT TCGTCAACTT GCCCACGGAG 
AATGAGCTGA CCTTCAACGC AGAGGAGTAT TCTGACATTT CTGAAATGCG CTCTCTGTCG 
GAGCTGCTGG GTCCATATGG GATGAAGTTC CTCAGCGAGA GTCTCATGTG GCACATCTCA 
TCCCAAGTTG CAGAACTGAA GAAACTTGTG GTGGACAATG TGGAGGTTTT AACCCAGATG 
AGGACCAGCT TTGACAAGCC GGACCATATG GCAGCACTCT TTAAGAGGCT AACATCGGTT 
GACAGCGTGC TGAAACGGAT GACCATCATT GGTGTGATTT TGTCGTTCCG CTCTCTGGCT 
CAGGAAGCTC TCCGAGATGT GCTCTCTTGC CACATTCCCT TCCTGGTGAG CTCCGTTGAA 
GACTTTAAAG ATCACATTCC TCGTGAAACT GACATGAAGG TGGCCATGAA CGTGTACGAG 
CTGTCTTCTG CTGCAGGCCT GCCCTGTGAG ATCGACCCTG CTCTAGTAGT GGCGCTGTCA 
TCCCAGAAGT CTGAAAATAT TAGCCCGGAG GAAGAGTACA AGATCGCCTG CCTGTTGATG 
GTCTTCGTGG CCGTTTCCAT GCCGACCCTG GCCAGCAACG TGATGTCACA GTACAGTCCC 
GCAATAGAAG GTCACTGCAA CAACATTCAC TGTCTGGCTA AAGCGATAAA CCAGATTGCC 
GCCGCTCTGT TCACGATCCA CAAGGGCAGC ATAGAGGATC GCCTCAAAGA ATTCCTCGCC 
CTCGCCTCCT CCAGCCTGCT GAAGATTGGA CAGGAGACGG ATAAAACCAC TACACGGAAC 
AGAGAGTCTG TTTACCTGCT CCTGGACATG ATCGTTCAAG AATCGCCATT CCTCACCATG 
GACCTGTTGG AGTCCTGCTT TCCATACGTC CTGCTCCGAA ACGCCTATCA CGCCGTATAC 
AAGCAGTCGG TATCTTCTTC AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167980.1
CDS	212..3598
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	XM_005167923.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167923.4

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC AACAGCTTGC ACAACTACAA 
AAAGAAAAGT CAGAAATACT GAAGAATTTA GCTCTATATT ACTTCACCTT TGTGGACGTT 
ATGGAATTTA AGGACCACGT TTGTGAACTG CTGAACACTA TTGATGCTTG TCAAGTTTTC 
TTTGACATTA CTGTAAATTT TGACCTCACA AAGAACTACC TGGACTTGGT AGTGACGTAC 
ACTACCCTGA TGTTGCTGCT TTCCCGAATT GAAGAGCGCA AAGCGATAAT CGGCCTCTAC 
AACTATGCCC ATGAAATGAC ACACGGTTCA AGCGATCGAG AATACCCCAG GCTTGGACAG 
ATGATTGTGG ATTACGAGAA CCCACTAAAA AAGATGATGG AGGAGTTTGT CCCTCATGGA 
AAATCGCTGT CAGATGCGCT GGTCTCCTTG CAGATGGTGT ACCCACGCCG GAATCTGTCT 
GCAGATCAGT GGAGAAACGC TCAGCTGCTC AGTTTGATTA GCGCTCCCAG CACTATGCTC 
AACCCAGCTC AATCTGACAC GATGCCCTGT GAATACCTAT CCTTGGACAC CATGGAGAAA 
TGGATCGTCT TCGGCTTCAT CTTGTGTCAT GCGGCTCTGA ACAGTGATCC TGCGGCTCTC 
AGTCTTTGGA AGTTGGCCCT TCAGAGCAGT ACCTGTCTCT GTCTGTTCAG GGACGAGGTC 
TTCCACATTC ACAAAGCTGC AGAGGATCTC TTCGTAAACA TTCGCGGTTA CAACAAACGT 
GTCAATGATA TTCGTGAATG CAAAGAATCT GCTCTTTCAC ATGCGGGCTC AATGCACAGA 
GAACGACGTA AGTTCTTGAG GTCTGCCCTG AAAGAGCTAG CCACAGTACT GGCAGATCAG 
CCTGGATTGC TGGGTCCGAA GGCTCTGTTT GTGTTCATGG CACTCTCCTT CGCCCGTGAT 
GAGATCATTT GGCTGCTCAG ACATGCCGAC AACATCCAAA AGAAAAGCAC AGATGACTTC 
ATTGACAAGC ACATTGCAGA GTTGATCTTC TACATGGAGG AGTTGAGGGC TCACGTCCGA 
AAGTACGGGC CGGTGATGCA GAGGTACTAT GTTCAGTACC TGTCCGGCTT TGACGCGGTG 
GTGCTCAATG AGCTTGTACA GAACCTGTCC GTGTGCCCAG AGGACGAGTC CATCATAATG 
TCGTCCTTCG TGAACACTAT GACCTCTCTC AGTGTTAAAC AAGTTGAAGA TGGCGAAGTG 
TTTGATTTCA GAGGGATGAG GCTGGACTGG TTCAGACTTC AGGCCTACAC CAGCGTGTCC 
AAGGCTTCGT TGGGACTGGC AGATCACAGA GAACTGGGAA AGATGATGAA CACCATCATC 
TTCCACACTA AGATGGTGGA CTCTCTGGTG GAGATGCTTG TGGAGACTTC TGACCTGTCC 
ATATTCTGTT TTTACAGCCG CGCCTTTGAA AAGATGTTCC AGCAGTGTCT TGAACTGCCG 
TCCCAGTCTC GATACTCCAT CTCCTTCCCA CTGCTCTGCA CCCACTTTAT GAGCTGCACT 
CATGAGCTCT GCCCTGAAGA GAGGCACCAC ATCGGAGACC GCAGCCTGTC CCTTTGCAAC 
ATGTTCCTGG ACGAGATGGC CAAGCAAGCC CGCAACCTCA TCACAGACAT ATGCACAGAG 
CAGTGCACCC TGAGCGACCA GCTACTGCCA AAACATTGTG CCAAAACCAT CAGTCAGGCC 
GTGAACAAGA AGTCGAAAAA GCAGACAGGG AAGAAGGGTG AACCGGAGCG AGAAAAACCT 
GGCGTGGAGA GCATGAGGAA GAACAGACTC CTGGTCACCA ATCTGGATAA ACTGCACACT 
GCTCTGTCCG AGCTCTGCTT CTCCATCAAC TACGTCCCCA ACATGATGGT CTGGGAGCAC 
ACCTTCACCC CACGCGAGTA CCTGACCTCC CACCTGGAGA TTCGCTTCAC TAAGTCTATA 
GTCGGGATGA CCATGTACAA CCAGACAACA CAGGAGATCG CCAAACCATC AGAGCTGCTC 
ACCAGCGTCC GGGCCTACAT GACAGTGCTG CAGTCCATTG AGAATTATGT GCAGATTGAC 
ATCACCAGAG TCTTCAACAA TGTGCTGCTG CAGCAAACCC AACACCTGGA CAGCCACGGA 
GAGCCAACCA TCACCAGCCT CTACACTAAC TGGTATCTGG AGACTTTACT GCGGCAGGTC 
AGCAACGGTC ACATCGCGTA CTTTCCTGCG ATGAAAGCCT TCGTCAACTT GCCCACGGAG 
AATGAGCTGA CCTTCAACGC AGAGGAGTAT TCTGACATTT CTGAAATGCG CTCTCTGTCG 
GAGCTGCTGG GTCCATATGG GATGAAGTTC CTCAGCGAGA GTCTCATGTG GCACATCTCA 
TCCCAAGTTG CAGAACTGAA GAAACTTGTG GTGGACAATG TGGAGGTTTT AACCCAGATG 
AGGACCAGCT TTGACAAGCC GGACCATATG GCAGCACTCT TTAAGAGGCT AACATCGGTT 
GACAGCGTGC TGAAACGGAT GACCATCATT GGTGTGATTT TGTCGTTCCG CTCTCTGGCT 
CAGGAAGCTC TCCGAGATGT GCTCTCTTGC CACATTCCCT TCCTGGTGAG CTCCGTTGAA 
GACTTTAAAG ATCACATTCC TCGTGAAACT GACATGAAGG TGGCCATGAA CGTGTACGAG 
CTGTCTTCTG CTGCAGGCCT GCCCTGTGAG ATCGACCCTG CTCTAGTAGT GGCGCTGTCA 
TCCCAGAAGT CTGAAAATAT TAGCCCGGAG GAAGAGTACA AGATCGCCTG CCTGTTGATG 
GTCTTCGTGG CCGTTTCCAT GCCGACCCTG GCCAGCAACG TGATGTCACA GTACAGTCCC 
GCAATAGAAG GTCACTGCAA CAACATTCAC TGTCTGGCTA AAGCGATAAA CCAGATTGCC 
GCCGCTCTGT TCACGATCCA CAAGGGCAGC ATAGAGGATC GCCTCAAAGA ATTCCTCGCC 
CTCGCCTCCT CCAGCCTGCT GAAGATTGGA CAGGAGACGG ATAAAACCAC TACACGGAAC 
AGAGAGTCTG TTTACCTGCT CCTGGACATG ATCGTTCAAG AATCGCCATT CCTCACCATG 
GACCTGTTGG AGTCCTGCTT TCCATACGTC CTGCTCCGAA ACGCCTATCA CGCCGTATAC 
AAGCAGTCGG TATCTTCTTC AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_005167923.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167980.1 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa15522
Clone ID Related Accession (Same CDS sequence)	XM_005167923.4 , XM_005167923.3
Accession Version	XM_005167923.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nck-associated protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC AACAGCTTGC ACAACTACAA 
AAAGAAAAGT CAGAAATACT GAAGAATTTA GCTCTATATT ACTTCACCTT TGTGGACGTT 
ATGGAATTTA AGGACCACGT TTGTGAACTG CTGAACACTA TTGATGCTTG TCAAGTTTTC 
TTTGACATTA CTGTAAATTT TGACCTCACA AAGAACTACC TGGACTTGGT AGTGACGTAC 
ACTACCCTGA TGTTGCTGCT TTCCCGAATT GAAGAGCGCA AAGCGATAAT CGGCCTCTAC 
AACTATGCCC ATGAAATGAC ACACGGTTCA AGCGATCGAG AATACCCCAG GCTTGGACAG 
ATGATTGTGG ATTACGAGAA CCCACTAAAA AAGATGATGG AGGAGTTTGT CCCTCATGGA 
AAATCGCTGT CAGATGCGCT GGTCTCCTTG CAGATGGTGT ACCCACGCCG GAATCTGTCT 
GCAGATCAGT GGAGAAACGC TCAGCTGCTC AGTTTGATTA GCGCTCCCAG CACTATGCTC 
AACCCAGCTC AATCTGACAC GATGCCCTGT GAATACCTAT CCTTGGACAC CATGGAGAAA 
TGGATCGTCT TCGGCTTCAT CTTGTGTCAT GCGGCTCTGA ACAGTGATCC TGCGGCTCTC 
AGTCTTTGGA AGTTGGCCCT TCAGAGCAGT ACCTGTCTCT GTCTGTTCAG GGACGAGGTC 
TTCCACATTC ACAAAGCTGC AGAGGATCTC TTCGTAAACA TTCGCGGTTA CAACAAACGT 
GTCAATGATA TTCGTGAATG CAAAGAATCT GCTCTTTCAC ATGCGGGCTC AATGCACAGA 
GAACGACGTA AGTTCTTGAG GTCTGCCCTG AAAGAGCTAG CCACAGTACT GGCAGATCAG 
CCTGGATTGC TGGGTCCGAA GGCTCTGTTT GTGTTCATGG CACTCTCCTT CGCCCGTGAT 
GAGATCATTT GGCTGCTCAG ACATGCCGAC AACATCCAAA AGAAAAGCAC AGATGACTTC 
ATTGACAAGC ACATTGCAGA GTTGATCTTC TACATGGAGG AGTTGAGGGC TCACGTCCGA 
AAGTACGGGC CGGTGATGCA GAGGTACTAT GTTCAGTACC TGTCCGGCTT TGACGCGGTG 
GTGCTCAATG AGCTTGTACA GAACCTGTCC GTGTGCCCAG AGGACGAGTC CATCATAATG 
TCGTCCTTCG TGAACACTAT GACCTCTCTC AGTGTTAAAC AAGTTGAAGA TGGCGAAGTG 
TTTGATTTCA GAGGGATGAG GCTGGACTGG TTCAGACTTC AGGCCTACAC CAGCGTGTCC 
AAGGCTTCGT TGGGACTGGC AGATCACAGA GAACTGGGAA AGATGATGAA CACCATCATC 
TTCCACACTA AGATGGTGGA CTCTCTGGTG GAGATGCTTG TGGAGACTTC TGACCTGTCC 
ATATTCTGTT TTTACAGCCG CGCCTTTGAA AAGATGTTCC AGCAGTGTCT TGAACTGCCG 
TCCCAGTCTC GATACTCCAT CTCCTTCCCA CTGCTCTGCA CCCACTTTAT GAGCTGCACT 
CATGAGCTCT GCCCTGAAGA GAGGCACCAC ATCGGAGACC GCAGCCTGTC CCTTTGCAAC 
ATGTTCCTGG ACGAGATGGC CAAGCAAGCC CGCAACCTCA TCACAGACAT ATGCACAGAG 
CAGTGCACCC TGAGCGACCA GCTACTGCCA AAACATTGTG CCAAAACCAT CAGTCAGGCC 
GTGAACAAGA AGTCGAAAAA GCAGACAGGG AAGAAGGGTG AACCGGAGCG AGAAAAACCT 
GGCGTGGAGA GCATGAGGAA GAACAGACTC CTGGTCACCA ATCTGGATAA ACTGCACACT 
GCTCTGTCCG AGCTCTGCTT CTCCATCAAC TACGTCCCCA ACATGATGGT CTGGGAGCAC 
ACCTTCACCC CACGCGAGTA CCTGACCTCC CACCTGGAGA TTCGCTTCAC TAAGTCTATA 
GTCGGGATGA CCATGTACAA CCAGACAACA CAGGAGATCG CCAAACCATC AGAGCTGCTC 
ACCAGCGTCC GGGCCTACAT GACAGTGCTG CAGTCCATTG AGAATTATGT GCAGATTGAC 
ATCACCAGAG TCTTCAACAA TGTGCTGCTG CAGCAAACCC AACACCTGGA CAGCCACGGA 
GAGCCAACCA TCACCAGCCT CTACACTAAC TGGTATCTGG AGACTTTACT GCGGCAGGTC 
AGCAACGGTC ACATCGCGTA CTTTCCTGCG ATGAAAGCCT TCGTCAACTT GCCCACGGAG 
AATGAGCTGA CCTTCAACGC AGAGGAGTAT TCTGACATTT CTGAAATGCG CTCTCTGTCG 
GAGCTGCTGG GTCCATATGG GATGAAGTTC CTCAGCGAGA GTCTCATGTG GCACATCTCA 
TCCCAAGTTG CAGAACTGAA GAAACTTGTG GTGGACAATG TGGAGGTTTT AACCCAGATG 
AGGACCAGCT TTGACAAGCC GGACCATATG GCAGCACTCT TTAAGAGGCT AACATCGGTT 
GACAGCGTGC TGAAACGGAT GACCATCATT GGTGTGATTT TGTCGTTCCG CTCTCTGGCT 
CAGGAAGCTC TCCGAGATGT GCTCTCTTGC CACATTCCCT TCCTGGTGAG CTCCGTTGAA 
GACTTTAAAG ATCACATTCC TCGTGAAACT GACATGAAGG TGGCCATGAA CGTGTACGAG 
CTGTCTTCTG CTGCAGGCCT GCCCTGTGAG ATCGACCCTG CTCTAGTAGT GGCGCTGTCA 
TCCCAGAAGT CTGAAAATAT TAGCCCGGAG GAAGAGTACA AGATCGCCTG CCTGTTGATG 
GTCTTCGTGG CCGTTTCCAT GCCGACCCTG GCCAGCAACG TGATGTCACA GTACAGTCCC 
GCAATAGAAG GTCACTGCAA CAACATTCAC TGTCTGGCTA AAGCGATAAA CCAGATTGCC 
GCCGCTCTGT TCACGATCCA CAAGGGCAGC ATAGAGGATC GCCTCAAAGA ATTCCTCGCC 
CTCGCCTCCT CCAGCCTGCT GAAGATTGGA CAGGAGACGG ATAAAACCAC TACACGGAAC 
AGAGAGTCTG TTTACCTGCT CCTGGACATG ATCGTTCAAG AATCGCCATT CCTCACCATG 
GACCTGTTGG AGTCCTGCTT TCCATACGTC CTGCTCCGAA ACGCCTATCA CGCCGTATAC 
AAGCAGTCGG TATCTTCTTC AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167980.1
CDS	231..3617
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	XM_005167923.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167923.3

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC AACAGCTTGC ACAACTACAA 
AAAGAAAAGT CAGAAATACT GAAGAATTTA GCTCTATATT ACTTCACCTT TGTGGACGTT 
ATGGAATTTA AGGACCACGT TTGTGAACTG CTGAACACTA TTGATGCTTG TCAAGTTTTC 
TTTGACATTA CTGTAAATTT TGACCTCACA AAGAACTACC TGGACTTGGT AGTGACGTAC 
ACTACCCTGA TGTTGCTGCT TTCCCGAATT GAAGAGCGCA AAGCGATAAT CGGCCTCTAC 
AACTATGCCC ATGAAATGAC ACACGGTTCA AGCGATCGAG AATACCCCAG GCTTGGACAG 
ATGATTGTGG ATTACGAGAA CCCACTAAAA AAGATGATGG AGGAGTTTGT CCCTCATGGA 
AAATCGCTGT CAGATGCGCT GGTCTCCTTG CAGATGGTGT ACCCACGCCG GAATCTGTCT 
GCAGATCAGT GGAGAAACGC TCAGCTGCTC AGTTTGATTA GCGCTCCCAG CACTATGCTC 
AACCCAGCTC AATCTGACAC GATGCCCTGT GAATACCTAT CCTTGGACAC CATGGAGAAA 
TGGATCGTCT TCGGCTTCAT CTTGTGTCAT GCGGCTCTGA ACAGTGATCC TGCGGCTCTC 
AGTCTTTGGA AGTTGGCCCT TCAGAGCAGT ACCTGTCTCT GTCTGTTCAG GGACGAGGTC 
TTCCACATTC ACAAAGCTGC AGAGGATCTC TTCGTAAACA TTCGCGGTTA CAACAAACGT 
GTCAATGATA TTCGTGAATG CAAAGAATCT GCTCTTTCAC ATGCGGGCTC AATGCACAGA 
GAACGACGTA AGTTCTTGAG GTCTGCCCTG AAAGAGCTAG CCACAGTACT GGCAGATCAG 
CCTGGATTGC TGGGTCCGAA GGCTCTGTTT GTGTTCATGG CACTCTCCTT CGCCCGTGAT 
GAGATCATTT GGCTGCTCAG ACATGCCGAC AACATCCAAA AGAAAAGCAC AGATGACTTC 
ATTGACAAGC ACATTGCAGA GTTGATCTTC TACATGGAGG AGTTGAGGGC TCACGTCCGA 
AAGTACGGGC CGGTGATGCA GAGGTACTAT GTTCAGTACC TGTCCGGCTT TGACGCGGTG 
GTGCTCAATG AGCTTGTACA GAACCTGTCC GTGTGCCCAG AGGACGAGTC CATCATAATG 
TCGTCCTTCG TGAACACTAT GACCTCTCTC AGTGTTAAAC AAGTTGAAGA TGGCGAAGTG 
TTTGATTTCA GAGGGATGAG GCTGGACTGG TTCAGACTTC AGGCCTACAC CAGCGTGTCC 
AAGGCTTCGT TGGGACTGGC AGATCACAGA GAACTGGGAA AGATGATGAA CACCATCATC 
TTCCACACTA AGATGGTGGA CTCTCTGGTG GAGATGCTTG TGGAGACTTC TGACCTGTCC 
ATATTCTGTT TTTACAGCCG CGCCTTTGAA AAGATGTTCC AGCAGTGTCT TGAACTGCCG 
TCCCAGTCTC GATACTCCAT CTCCTTCCCA CTGCTCTGCA CCCACTTTAT GAGCTGCACT 
CATGAGCTCT GCCCTGAAGA GAGGCACCAC ATCGGAGACC GCAGCCTGTC CCTTTGCAAC 
ATGTTCCTGG ACGAGATGGC CAAGCAAGCC CGCAACCTCA TCACAGACAT ATGCACAGAG 
CAGTGCACCC TGAGCGACCA GCTACTGCCA AAACATTGTG CCAAAACCAT CAGTCAGGCC 
GTGAACAAGA AGTCGAAAAA GCAGACAGGG AAGAAGGGTG AACCGGAGCG AGAAAAACCT 
GGCGTGGAGA GCATGAGGAA GAACAGACTC CTGGTCACCA ATCTGGATAA ACTGCACACT 
GCTCTGTCCG AGCTCTGCTT CTCCATCAAC TACGTCCCCA ACATGATGGT CTGGGAGCAC 
ACCTTCACCC CACGCGAGTA CCTGACCTCC CACCTGGAGA TTCGCTTCAC TAAGTCTATA 
GTCGGGATGA CCATGTACAA CCAGACAACA CAGGAGATCG CCAAACCATC AGAGCTGCTC 
ACCAGCGTCC GGGCCTACAT GACAGTGCTG CAGTCCATTG AGAATTATGT GCAGATTGAC 
ATCACCAGAG TCTTCAACAA TGTGCTGCTG CAGCAAACCC AACACCTGGA CAGCCACGGA 
GAGCCAACCA TCACCAGCCT CTACACTAAC TGGTATCTGG AGACTTTACT GCGGCAGGTC 
AGCAACGGTC ACATCGCGTA CTTTCCTGCG ATGAAAGCCT TCGTCAACTT GCCCACGGAG 
AATGAGCTGA CCTTCAACGC AGAGGAGTAT TCTGACATTT CTGAAATGCG CTCTCTGTCG 
GAGCTGCTGG GTCCATATGG GATGAAGTTC CTCAGCGAGA GTCTCATGTG GCACATCTCA 
TCCCAAGTTG CAGAACTGAA GAAACTTGTG GTGGACAATG TGGAGGTTTT AACCCAGATG 
AGGACCAGCT TTGACAAGCC GGACCATATG GCAGCACTCT TTAAGAGGCT AACATCGGTT 
GACAGCGTGC TGAAACGGAT GACCATCATT GGTGTGATTT TGTCGTTCCG CTCTCTGGCT 
CAGGAAGCTC TCCGAGATGT GCTCTCTTGC CACATTCCCT TCCTGGTGAG CTCCGTTGAA 
GACTTTAAAG ATCACATTCC TCGTGAAACT GACATGAAGG TGGCCATGAA CGTGTACGAG 
CTGTCTTCTG CTGCAGGCCT GCCCTGTGAG ATCGACCCTG CTCTAGTAGT GGCGCTGTCA 
TCCCAGAAGT CTGAAAATAT TAGCCCGGAG GAAGAGTACA AGATCGCCTG CCTGTTGATG 
GTCTTCGTGG CCGTTTCCAT GCCGACCCTG GCCAGCAACG TGATGTCACA GTACAGTCCC 
GCAATAGAAG GTCACTGCAA CAACATTCAC TGTCTGGCTA AAGCGATAAA CCAGATTGCC 
GCCGCTCTGT TCACGATCCA CAAGGGCAGC ATAGAGGATC GCCTCAAAGA ATTCCTCGCC 
CTCGCCTCCT CCAGCCTGCT GAAGATTGGA CAGGAGACGG ATAAAACCAC TACACGGAAC 
AGAGAGTCTG TTTACCTGCT CCTGGACATG ATCGTTCAAG AATCGCCATT CCTCACCATG 
GACCTGTTGG AGTCCTGCTT TCCATACGTC CTGCTCCGAA ACGCCTATCA CGCCGTATAC 
AAGCAGTCGG TATCTTCTTC AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_005167924.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167981.1 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa15523
Clone ID Related Accession (Same CDS sequence)	XM_005167924.3 , XM_005167924.4
Accession Version	XM_005167924.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nck-associated protein 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA 
AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA 
TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC 
ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC 
CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT 
GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT 
GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG 
CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT 
CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA 
GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC 
GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT 
TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC 
ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT 
GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA 
CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA 
TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC 
ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC 
AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC 
GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC 
AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC 
TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT 
TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT 
TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC 
ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC 
TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG 
TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG 
CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC 
CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC 
ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC 
AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG 
GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG 
TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC 
ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG 
ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC 
GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC 
AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA 
ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC 
GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG 
CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG 
CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA 
GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC 
AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC 
GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA 
GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT 
AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT 
TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG 
AAGTCTGAAA ATATTAGCCC GGAGGAAGAG TACAAGATCG CCTGCCTGTT GATGGTCTTC 
GTGGCCGTTT CCATGCCGAC CCTGGCCAGC AACGTGATGT CACAGTACAG TCCCGCAATA 
GAAGGTCACT GCAACAACAT TCACTGTCTG GCTAAAGCGA TAAACCAGAT TGCCGCCGCT 
CTGTTCACGA TCCACAAGGG CAGCATAGAG GATCGCCTCA AAGAATTCCT CGCCCTCGCC 
TCCTCCAGCC TGCTGAAGAT TGGACAGGAG ACGGATAAAA CCACTACACG GAACAGAGAG 
TCTGTTTACC TGCTCCTGGA CATGATCGTT CAAGAATCGC CATTCCTCAC CATGGACCTG 
TTGGAGTCCT GCTTTCCATA CGTCCTGCTC CGAAACGCCT ATCACGCCGT ATACAAGCAG 
TCGGTATCTT CTTCAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167981.1
CDS	230..3610
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	XM_005167924.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167924.3

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA 
AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA 
TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC 
ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC 
CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT 
GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT 
GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG 
CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT 
CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA 
GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC 
GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT 
TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC 
ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT 
GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA 
CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA 
TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC 
ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC 
AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC 
GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC 
AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC 
TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT 
TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT 
TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC 
ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC 
TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG 
TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG 
CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC 
CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC 
ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC 
AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG 
GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG 
TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC 
ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG 
ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC 
GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC 
AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA 
ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC 
GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG 
CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG 
CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA 
GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC 
AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC 
GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA 
GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT 
AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT 
TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG 
AAGTCTGAAA ATATTAGCCC GGAGGAAGAG TACAAGATCG CCTGCCTGTT GATGGTCTTC 
GTGGCCGTTT CCATGCCGAC CCTGGCCAGC AACGTGATGT CACAGTACAG TCCCGCAATA 
GAAGGTCACT GCAACAACAT TCACTGTCTG GCTAAAGCGA TAAACCAGAT TGCCGCCGCT 
CTGTTCACGA TCCACAAGGG CAGCATAGAG GATCGCCTCA AAGAATTCCT CGCCCTCGCC 
TCCTCCAGCC TGCTGAAGAT TGGACAGGAG ACGGATAAAA CCACTACACG GAACAGAGAG 
TCTGTTTACC TGCTCCTGGA CATGATCGTT CAAGAATCGC CATTCCTCAC CATGGACCTG 
TTGGAGTCCT GCTTTCCATA CGTCCTGCTC CGAAACGCCT ATCACGCCGT ATACAAGCAG 
TCGGTATCTT CTTCAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_005167924.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167981.1 Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa15523
Clone ID Related Accession (Same CDS sequence)	XM_005167924.3 , XM_005167924.4
Accession Version	XM_005167924.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nck-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167924.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA 
AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA 
TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC 
ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC 
CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT 
GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT 
GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG 
CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT 
CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA 
GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC 
GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT 
TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC 
ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT 
GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA 
CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA 
TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC 
ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC 
AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC 
GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC 
AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC 
TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT 
TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT 
TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC 
ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC 
TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG 
TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG 
CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC 
CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC 
ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC 
AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG 
GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG 
TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC 
ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG 
ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC 
GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC 
AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA 
ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC 
GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG 
CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG 
CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA 
GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC 
AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC 
GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA 
GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT 
AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT 
TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG 
AAGTCTGAAA ATATTAGCCC GGAGGAAGAG TACAAGATCG CCTGCCTGTT GATGGTCTTC 
GTGGCCGTTT CCATGCCGAC CCTGGCCAGC AACGTGATGT CACAGTACAG TCCCGCAATA 
GAAGGTCACT GCAACAACAT TCACTGTCTG GCTAAAGCGA TAAACCAGAT TGCCGCCGCT 
CTGTTCACGA TCCACAAGGG CAGCATAGAG GATCGCCTCA AAGAATTCCT CGCCCTCGCC 
TCCTCCAGCC TGCTGAAGAT TGGACAGGAG ACGGATAAAA CCACTACACG GAACAGAGAG 
TCTGTTTACC TGCTCCTGGA CATGATCGTT CAAGAATCGC CATTCCTCAC CATGGACCTG 
TTGGAGTCCT GCTTTCCATA CGTCCTGCTC CGAAACGCCT ATCACGCCGT ATACAAGCAG 
TCGGTATCTT CTTCAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167981.1
CDS	212..3592
Translation	MSRGAMQPSQQKLAEKLTILNDRGIGMLTRIYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDACQVFFDITVNFDLTKNYLDLVVTYTTLMLLLSRIEERKAIIGLYNYAHEMTHGSSDREYPRLGQMIVDYENPLKKMMEEFVPHGKSLSDALVSLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDTMEKWIVFGFILCHAALNSDPAALSLWKLALQSSTCLCLFRDEVFHIHKAAEDLFVNIRGYNKRVNDIRECKESALSHAGSMHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIQKKSTDDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLLVTNLDKLHTALSELCFSINYVPNMMVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQTTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVDNVEVLTQMRTSFDKPDHMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSCHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSMPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVSSSA

Target ORF information:

RefSeq Version	XM_005167924.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167924.4

ORF Insert Sequence:

ATGTCTCGGG GCGCGATGCA GCCGAGCCAG CAGAAGCTGG CGGAGAAATT GACGATCCTC 
AACGACCGCG GCATCGGGAT GCTCACTCGC ATCTACAACA TCAAAAAGGC CTGTGGAGAT 
CCCAAGGCAA AGCCTTCCTA CCTGATTGAC AAAAACCTGG AGTCAGCCGT GAAATTCATT 
GTCAGAAAAT TCCCAGCCGT TGAGACCCGC AACAACAATC TTGCACAACT ACAAAAAGAA 
AAGTCAGAAA TACTGAAGAA TTTAGCTCTA TATTACTTCA CCTTTGTGGA CGTTATGGAA 
TTTAAGGACC ACGTTTGTGA ACTGCTGAAC ACTATTGATG CTTGTCAAGT TTTCTTTGAC 
ATTACTGTAA ATTTTGACCT CACAAAGAAC TACCTGGACT TGGTAGTGAC GTACACTACC 
CTGATGTTGC TGCTTTCCCG AATTGAAGAG CGCAAAGCGA TAATCGGCCT CTACAACTAT 
GCCCATGAAA TGACACACGG TTCAAGCGAT CGAGAATACC CCAGGCTTGG ACAGATGATT 
GTGGATTACG AGAACCCACT AAAAAAGATG ATGGAGGAGT TTGTCCCTCA TGGAAAATCG 
CTGTCAGATG CGCTGGTCTC CTTGCAGATG GTGTACCCAC GCCGGAATCT GTCTGCAGAT 
CAGTGGAGAA ACGCTCAGCT GCTCAGTTTG ATTAGCGCTC CCAGCACTAT GCTCAACCCA 
GCTCAATCTG ACACGATGCC CTGTGAATAC CTATCCTTGG ACACCATGGA GAAATGGATC 
GTCTTCGGCT TCATCTTGTG TCATGCGGCT CTGAACAGTG ATCCTGCGGC TCTCAGTCTT 
TGGAAGTTGG CCCTTCAGAG CAGTACCTGT CTCTGTCTGT TCAGGGACGA GGTCTTCCAC 
ATTCACAAAG CTGCAGAGGA TCTCTTCGTA AACATTCGCG GTTACAACAA ACGTGTCAAT 
GATATTCGTG AATGCAAAGA ATCTGCTCTT TCACATGCGG GCTCAATGCA CAGAGAACGA 
CGTAAGTTCT TGAGGTCTGC CCTGAAAGAG CTAGCCACAG TACTGGCAGA TCAGCCTGGA 
TTGCTGGGTC CGAAGGCTCT GTTTGTGTTC ATGGCACTCT CCTTCGCCCG TGATGAGATC 
ATTTGGCTGC TCAGACATGC CGACAACATC CAAAAGAAAA GCACAGATGA CTTCATTGAC 
AAGCACATTG CAGAGTTGAT CTTCTACATG GAGGAGTTGA GGGCTCACGT CCGAAAGTAC 
GGGCCGGTGA TGCAGAGGTA CTATGTTCAG TACCTGTCCG GCTTTGACGC GGTGGTGCTC 
AATGAGCTTG TACAGAACCT GTCCGTGTGC CCAGAGGACG AGTCCATCAT AATGTCGTCC 
TTCGTGAACA CTATGACCTC TCTCAGTGTT AAACAAGTTG AAGATGGCGA AGTGTTTGAT 
TTCAGAGGGA TGAGGCTGGA CTGGTTCAGA CTTCAGGCCT ACACCAGCGT GTCCAAGGCT 
TCGTTGGGAC TGGCAGATCA CAGAGAACTG GGAAAGATGA TGAACACCAT CATCTTCCAC 
ACTAAGATGG TGGACTCTCT GGTGGAGATG CTTGTGGAGA CTTCTGACCT GTCCATATTC 
TGTTTTTACA GCCGCGCCTT TGAAAAGATG TTCCAGCAGT GTCTTGAACT GCCGTCCCAG 
TCTCGATACT CCATCTCCTT CCCACTGCTC TGCACCCACT TTATGAGCTG CACTCATGAG 
CTCTGCCCTG AAGAGAGGCA CCACATCGGA GACCGCAGCC TGTCCCTTTG CAACATGTTC 
CTGGACGAGA TGGCCAAGCA AGCCCGCAAC CTCATCACAG ACATATGCAC AGAGCAGTGC 
ACCCTGAGCG ACCAGCTACT GCCAAAACAT TGTGCCAAAA CCATCAGTCA GGCCGTGAAC 
AAGAAGTCGA AAAAGCAGAC AGGGAAGAAG GGTGAACCGG AGCGAGAAAA ACCTGGCGTG 
GAGAGCATGA GGAAGAACAG ACTCCTGGTC ACCAATCTGG ATAAACTGCA CACTGCTCTG 
TCCGAGCTCT GCTTCTCCAT CAACTACGTC CCCAACATGA TGGTCTGGGA GCACACCTTC 
ACCCCACGCG AGTACCTGAC CTCCCACCTG GAGATTCGCT TCACTAAGTC TATAGTCGGG 
ATGACCATGT ACAACCAGAC AACACAGGAG ATCGCCAAAC CATCAGAGCT GCTCACCAGC 
GTCCGGGCCT ACATGACAGT GCTGCAGTCC ATTGAGAATT ATGTGCAGAT TGACATCACC 
AGAGTCTTCA ACAATGTGCT GCTGCAGCAA ACCCAACACC TGGACAGCCA CGGAGAGCCA 
ACCATCACCA GCCTCTACAC TAACTGGTAT CTGGAGACTT TACTGCGGCA GGTCAGCAAC 
GGTCACATCG CGTACTTTCC TGCGATGAAA GCCTTCGTCA ACTTGCCCAC GGAGAATGAG 
CTGACCTTCA ACGCAGAGGA GTATTCTGAC ATTTCTGAAA TGCGCTCTCT GTCGGAGCTG 
CTGGGTCCAT ATGGGATGAA GTTCCTCAGC GAGAGTCTCA TGTGGCACAT CTCATCCCAA 
GTTGCAGAAC TGAAGAAACT TGTGGTGGAC AATGTGGAGG TTTTAACCCA GATGAGGACC 
AGCTTTGACA AGCCGGACCA TATGGCAGCA CTCTTTAAGA GGCTAACATC GGTTGACAGC 
GTGCTGAAAC GGATGACCAT CATTGGTGTG ATTTTGTCGT TCCGCTCTCT GGCTCAGGAA 
GCTCTCCGAG ATGTGCTCTC TTGCCACATT CCCTTCCTGG TGAGCTCCGT TGAAGACTTT 
AAAGATCACA TTCCTCGTGA AACTGACATG AAGGTGGCCA TGAACGTGTA CGAGCTGTCT 
TCTGCTGCAG GCCTGCCCTG TGAGATCGAC CCTGCTCTAG TAGTGGCGCT GTCATCCCAG 
AAGTCTGAAA ATATTAGCCC GGAGGAAGAG TACAAGATCG CCTGCCTGTT GATGGTCTTC 
GTGGCCGTTT CCATGCCGAC CCTGGCCAGC AACGTGATGT CACAGTACAG TCCCGCAATA 
GAAGGTCACT GCAACAACAT TCACTGTCTG GCTAAAGCGA TAAACCAGAT TGCCGCCGCT 
CTGTTCACGA TCCACAAGGG CAGCATAGAG GATCGCCTCA AAGAATTCCT CGCCCTCGCC 
TCCTCCAGCC TGCTGAAGAT TGGACAGGAG ACGGATAAAA CCACTACACG GAACAGAGAG 
TCTGTTTACC TGCTCCTGGA CATGATCGTT CAAGAATCGC CATTCCTCAC CATGGACCTG 
TTGGAGTCCT GCTTTCCATA CGTCCTGCTC CGAAACGCCT ATCACGCCGT ATACAAGCAG 
TCGGTATCTT CTTCAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NCKAP1
Entrez Gene ID	561894
Full Name	NCK-associated protein 1
Synonyms	si:dkey-234n3.1,wu:fd05c01
General protein information	Preferred Names NCK-associated protein 1 Names nck-associated protein 1 NAP 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

Canis lupus familiaris (dog)	NCKAP1	XP_535990.3
Xenopus tropicalis (tropical clawed frog)	nckap1	XP_004917966.1
Pan troglodytes (chimpanzee)	NCKAP1	XP_001148485.2
Rattus norvegicus (Norway rat)	Nckap1	NP_113806.1
Drosophila melanogaster (fruit fly)	Hem	NP_524214.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004946	XP_315044.2
	NCKAP1	XP_001099879.2
Bos taurus (cattle)	LOC101907541	XP_005202363.1
Mus musculus (house mouse)	Nckap1	NP_058661.1
Arabidopsis thaliana (thale cress)	GRL	NP_001031485.1
Oryza sativa (rice)	Os08g0544500	NP_001062406.2
Homo sapiens (human)	NCKAP1	NP_995314.1
Gallus gallus (chicken)	NCKAP1	XP_001232275.2
Danio rerio (zebrafish)	nckap1	NP_001131137.1
Caenorhabditis elegans (roundworm)	gex-3	NP_502368.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NCKAP1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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