trim71 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trim71
Entrez Gene ID	561754
Full Name	tripartite motif containing 71, E3 ubiquitin protein ligase
Synonyms	cb205,fb93a12,lin41,sb:cb205,wu:fb93a12
General protein information	Preferred Names tripartite motif containing 71, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase TRIM71 RING-type E3 ubiquitin transferase TRIM71 lin-41 homolog protein lin-41 homolog tripartite motif-containing 71 tripartite motif-containing protein 71
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001301331.1	NP_001288260.1	E3 ubiquitin-protein ligase TRIM71

Pathways from BioSystems

Homologies

	TRIM71	XP_001098706.1
Bos taurus (cattle)	TRIM71	XP_002707823.1
Gallus gallus (chicken)	TRIM71	NP_001032352.2
Pan troglodytes (chimpanzee)	TRIM71	XP_516352.3
Rattus norvegicus (Norway rat)	Trim71	NP_001178730.1
Danio rerio (zebrafish)	trim71	XP_690252.5
Caenorhabditis elegans (roundworm)	lin-41	NP_001020998.1
Mus musculus (house mouse)	Trim71	NP_001035968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005125	XP_314006.4
Homo sapiens (human)	TRIM71	NP_001034200.1
Canis lupus familiaris (dog)	TRIM71	XP_534214.3
Drosophila melanogaster (fruit fly)	wech	NP_001137614.1
Xenopus tropicalis (tropical clawed frog)	LOC100486898	XP_004915429.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The heterochronic genes lin-28a and lin-28b play an essential and evolutionarily conserved role in early zebrafish development.
Ouchi Y, Yamamoto J, Iwamoto T
PloS one9(2)e88086(2014)

Origin and evolution of TRIM proteins: new insights from the complete TRIM repertoire of zebrafish and pufferfish.
Boudinot P, van der Aa LM, Jouneau L, Du Pasquier L, Pontarotti P, Briolat V, Benmansour A, Levraud JP
PloS one6(7)e22022(2011)

Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs.
Hsu RJ, Yang HJ, Tsai HJ
Nucleic acids research37(10)e77(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Repression of human TRIM71 and the zebrafish lin-41 ortholog was abolished when predicted let-7 target sites were mutated. Regulation of TRIM71 expression by let-7 seems to have been evolutionarily conserved.
Title: Human TRIM71 and its nematode homologue are targets of let-7 microRNA and its zebrafish orthologue is essential for development.

The following trim71 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trim71 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa43584	NM_001301331.1 Latest version!	Danio rerio tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trim71 ( NM_001301331.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001288260.1 Danio rerio tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), mRNA.

Price & Availability↑

CloneID	ODa43584
Clone ID Related Accession (Same CDS sequence)	NM_001301331.1
Accession Version	NM_001301331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase TRIM71
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01061337.1 and BX511173.7. On Aug 5, 2014 this sequence version replaced XM_685160.6. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01042008.1, GDQH01009343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476737, SAMEA4476751 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGCGTCTT TCCCAGACTC CGACCTGCAA ACATGTCCGC TCTGCAAGGA GCTGTGCGGC 
TGCTCTGCTC CGATCTCCTC CAACTCATCT ACCTCCTCCT CGTCCTCTCA GACCTCCAAC 
TCCTCATCTA CCTCATCCAC CAGGAGACTG CACGTCCTGC CCTGTCTGCA CGCCTTCTGC 
AGGCAGTGCC TGGAGGGCCA GCGGAGCCCC GGCGACCCCC TGAAGCTCAG ATGCCCCACA 
TGCGACCAAA AGGTGTCTCT GTCGGAGTCG GGCGTGGACG CGCTGCCCTC CTCCAACTTT 
CTCTTCAGCA ACCTGCTGGA CGTGGTGGTG TCCGCTGAAG AGCAGGGCAA GAACGGGCGG 
TCTTCTGCGG TGGTCCATCA CGGTGGTCTT CTGCGACCCC AGCACCTCTC GGACCCCCAG 
TGCAGCTCTT GTGACGAGGG CAACCCCGCT ACCTCCCACT GCCTGGACTG CCAGGAGTAC 
CTGTGCGACA ACTGCGTCAG GGCCCACCAG AGGGTCCGAC TCACCAAAGA CCACTTCATC 
GAGGGCCTGC TGGAGAGCCT ACACCTGGCC AACCGCACCA ACAACTCCAA CACCCCGGTC 
TCCATCTCCC AGTCCTTCCA CAACAGCTTC TCCATGCTCA ATGTTTTCCA GGAGAGGATG 
GACTTTTGCC AGCACCATGA TGACGCGGTC CTGCGGTTCT TCTGCGACAG CTGCACCGTG 
CCGATCTGTA GAGAGTGCAG TTTGGGCAGA CACGCGGGTC ACAGCTTCAC ATACCTACAG 
GACGCATTGC AGGATTCCAG AGCGCTGACC ATCCAGCTCC TCGCAGACGC GCAGCAGGGC 
AGACAGGCCA TACAGCTGAG CATCGAGAAA GCCCAGGCCA TTGCTGAACA GGTGGAGCTG 
AAGGCTAAGG TTGTGCAGTC AGAAGTAAAA ACAATCACTC TCCGTCATAA GAAAGCCCTG 
GAAGAACGGG AATGCGAGCT GCTTTGGAAG GTGGAGAAGA TCCGCCAGGT GAAGGCAAAG 
TCACTCTACC TGCAGGTTGA GAAACTACAT CAGAATCTCA CGAAGCTGGA TAGTACTATT 
GCCACAGTGA CACAGGTTCT TGAAGAGGGC CGTAGCATAG ACGTCCTGCT AGCCCGCGAG 
CACATGTTAA ATCAACTACA GGAGCTCAAA TCCCTCCGTT GCATTCTTCA GCCCCAAGAA 
GACGATCGCA TCATGTTCAC CCCTCCAGAC CAGGCTCTTC TTATTGCCAT CAAGTCCTTG 
GGCCTCATCA GCAGTGGAGC TTTCGCCACA GCATCCAAAG CTCACGGAGA AGGCATCAAG 
AGAGCGCTGC AAGGAAAGCC AGCATCCTTT ACCGTTGTAG GATACGATCA TGATGGAGAG 
CCTAGATTGT CCGGTGGTGA CAGTGTCTCG GTCGTGCTTA TGAGTCCGGA TGGTAATCTG 
TCCAGTGCAG AGGTTTCCGA CCACCAAGAT GGAACGTACA CGGTCAGCTA TCTGCCTAAA 
GGCGAGGGGG AGCATTTGCT GTCTGTGCTT ATATGCAATC AGCACATTGA GGGAAGTCCT 
TTCAAGGTGA TGGTGAAGTC GGGTCGCAGT TACGGTGGTG TCGGTTTGCC CATGGCGTCT 
TTCGGTGGAG AAGGGGATGG GGACGGGCAA CTGTGTCGAC CTTGGGGGAT CTGCGTGGAT 
AAGGAGGGCT ATGTGGTGGT CGCTGACCGA AGCAACAACC GTGTGCAGAT CTTCAAGCCT 
TGTGGCACAT TCCACCACAA GTTTGGCACT TTGGGCTCAC GACCGGGCCA GTTTGACCGC 
CCTGCCGGGG TCGCCTGTGA CAGCCAGAGG AGAATCATTG TGGCAGATAA GGACAATCAC 
CGCATCCAGA TCTTCACCTT CGATGGGCAG TTTCTTCTCA AGTTCGGTGA GAAGGGAACC 
AAAAATGGTC AGTTCAACTA CCCATGGGAT GTGGCCGTCA ACTTCGAGGG GAAGATCTTG 
GTTTCAGATA CACGCAACCA CCGCGTGCAA CTCTTTGGGC CTGACGGAAC TTTCCTCAAC 
AAGTATGGAT TCGAAGGTGC CCTATGGAAA CATTTCGACT CCCCTCGTGG CGTGGCCTTC 
AATCAGGAAG GTCACCTTGT TGTGACTGAC TTCAACAATC ATCGACTGCT CGTCATCAGG 
CCTGACTGCC AGTCTGCACG CTTCCTTGGA TCCGAGGGAA CTGGGAATGG CCAGTTCTTG 
CGGCCACAAG GTGTAGCCGT GGACCAGGAG GATCGTATTA TCGTGGCGGA CTCCCGCAAC 
CATCGCATTC AGGTGTTCGA ACCCAACGGC AACTTTTTAT GCAAGTTTGG GACTCACGGT 
AATGGCTTCG GGCAAATGGA TCGCCCCTCT GGTATAGCAG TGACCCCAGA TGGAGTGATT 
GTGGCCGTGG ATTTCGGAAA CAACCGAATC CTCATGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288260.1
CDS	771..3212
Translation	MASFPDSDLQTCPLCKELCGCSAPISSNSSTSSSSSQTSNSSSTSSTRRLHVLPCLHAFCRQCLEGQRSPGDPLKLRCPTCDQKVSLSESGVDALPSSNFLFSNLLDVVVSAEEQGKNGRSSAVVHHGGLLRPQHLSDPQCSSCDEGNPATSHCLDCQEYLCDNCVRAHQRVRLTKDHFIEGLLESLHLANRTNNSNTPVSISQSFHNSFSMLNVFQERMDFCQHHDDAVLRFFCDSCTVPICRECSLGRHAGHSFTYLQDALQDSRALTIQLLADAQQGRQAIQLSIEKAQAIAEQVELKAKVVQSEVKTITLRHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLHQNLTKLDSTIATVTQVLEEGRSIDVLLAREHMLNQLQELKSLRCILQPQEDDRIMFTPPDQALLIAIKSLGLISSGAFATASKAHGEGIKRALQGKPASFTVVGYDHDGEPRLSGGDSVSVVLMSPDGNLSSAEVSDHQDGTYTVSYLPKGEGEHLLSVLICNQHIEGSPFKVMVKSGRSYGGVGLPMASFGGEGDGDGQLCRPWGICVDKEGYVVVADRSNNRVQIFKPCGTFHHKFGTLGSRPGQFDRPAGVACDSQRRIIVADKDNHRIQIFTFDGQFLLKFGEKGTKNGQFNYPWDVAVNFEGKILVSDTRNHRVQLFGPDGTFLNKYGFEGALWKHFDSPRGVAFNQEGHLVVTDFNNHRLLVIRPDCQSARFLGSEGTGNGQFLRPQGVAVDQEDRIIVADSRNHRIQVFEPNGNFLCKFGTHGNGFGQMDRPSGIAVTPDGVIVAVDFGNNRILMF

Target ORF information:

RefSeq Version	NM_001301331.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301331.1

ORF Insert Sequence:

ATGGCGTCTT TCCCAGACTC CGACCTGCAA ACATGTCCGC TCTGCAAGGA GCTGTGCGGC 
TGCTCTGCTC CGATCTCCTC CAACTCATCT ACCTCCTCCT CGTCCTCTCA GACCTCCAAC 
TCCTCATCTA CCTCATCCAC CAGGAGACTG CACGTCCTGC CCTGTCTGCA CGCCTTCTGC 
AGGCAGTGCC TGGAGGGCCA GCGGAGCCCC GGCGACCCCC TGAAGCTCAG ATGCCCCACA 
TGCGACCAAA AGGTGTCTCT GTCGGAGTCG GGCGTGGACG CGCTGCCCTC CTCCAACTTT 
CTCTTCAGCA ACCTGCTGGA CGTGGTGGTG TCCGCTGAAG AGCAGGGCAA GAACGGGCGG 
TCTTCTGCGG TGGTCCATCA CGGTGGTCTT CTGCGACCCC AGCACCTCTC GGACCCCCAG 
TGCAGCTCTT GTGACGAGGG CAACCCCGCT ACCTCCCACT GCCTGGACTG CCAGGAGTAC 
CTGTGCGACA ACTGCGTCAG GGCCCACCAG AGGGTCCGAC TCACCAAAGA CCACTTCATC 
GAGGGCCTGC TGGAGAGCCT ACACCTGGCC AACCGCACCA ACAACTCCAA CACCCCGGTC 
TCCATCTCCC AGTCCTTCCA CAACAGCTTC TCCATGCTCA ATGTTTTCCA GGAGAGGATG 
GACTTTTGCC AGCACCATGA TGACGCGGTC CTGCGGTTCT TCTGCGACAG CTGCACCGTG 
CCGATCTGTA GAGAGTGCAG TTTGGGCAGA CACGCGGGTC ACAGCTTCAC ATACCTACAG 
GACGCATTGC AGGATTCCAG AGCGCTGACC ATCCAGCTCC TCGCAGACGC GCAGCAGGGC 
AGACAGGCCA TACAGCTGAG CATCGAGAAA GCCCAGGCCA TTGCTGAACA GGTGGAGCTG 
AAGGCTAAGG TTGTGCAGTC AGAAGTAAAA ACAATCACTC TCCGTCATAA GAAAGCCCTG 
GAAGAACGGG AATGCGAGCT GCTTTGGAAG GTGGAGAAGA TCCGCCAGGT GAAGGCAAAG 
TCACTCTACC TGCAGGTTGA GAAACTACAT CAGAATCTCA CGAAGCTGGA TAGTACTATT 
GCCACAGTGA CACAGGTTCT TGAAGAGGGC CGTAGCATAG ACGTCCTGCT AGCCCGCGAG 
CACATGTTAA ATCAACTACA GGAGCTCAAA TCCCTCCGTT GCATTCTTCA GCCCCAAGAA 
GACGATCGCA TCATGTTCAC CCCTCCAGAC CAGGCTCTTC TTATTGCCAT CAAGTCCTTG 
GGCCTCATCA GCAGTGGAGC TTTCGCCACA GCATCCAAAG CTCACGGAGA AGGCATCAAG 
AGAGCGCTGC AAGGAAAGCC AGCATCCTTT ACCGTTGTAG GATACGATCA TGATGGAGAG 
CCTAGATTGT CCGGTGGTGA CAGTGTCTCG GTCGTGCTTA TGAGTCCGGA TGGTAATCTG 
TCCAGTGCAG AGGTTTCCGA CCACCAAGAT GGAACGTACA CGGTCAGCTA TCTGCCTAAA 
GGCGAGGGGG AGCATTTGCT GTCTGTGCTT ATATGCAATC AGCACATTGA GGGAAGTCCT 
TTCAAGGTGA TGGTGAAGTC GGGTCGCAGT TACGGTGGTG TCGGTTTGCC CATGGCGTCT 
TTCGGTGGAG AAGGGGATGG GGACGGGCAA CTGTGTCGAC CTTGGGGGAT CTGCGTGGAT 
AAGGAGGGCT ATGTGGTGGT CGCTGACCGA AGCAACAACC GTGTGCAGAT CTTCAAGCCT 
TGTGGCACAT TCCACCACAA GTTTGGCACT TTGGGCTCAC GACCGGGCCA GTTTGACCGC 
CCTGCCGGGG TCGCCTGTGA CAGCCAGAGG AGAATCATTG TGGCAGATAA GGACAATCAC 
CGCATCCAGA TCTTCACCTT CGATGGGCAG TTTCTTCTCA AGTTCGGTGA GAAGGGAACC 
AAAAATGGTC AGTTCAACTA CCCATGGGAT GTGGCCGTCA ACTTCGAGGG GAAGATCTTG 
GTTTCAGATA CACGCAACCA CCGCGTGCAA CTCTTTGGGC CTGACGGAAC TTTCCTCAAC 
AAGTATGGAT TCGAAGGTGC CCTATGGAAA CATTTCGACT CCCCTCGTGG CGTGGCCTTC 
AATCAGGAAG GTCACCTTGT TGTGACTGAC TTCAACAATC ATCGACTGCT CGTCATCAGG 
CCTGACTGCC AGTCTGCACG CTTCCTTGGA TCCGAGGGAA CTGGGAATGG CCAGTTCTTG 
CGGCCACAAG GTGTAGCCGT GGACCAGGAG GATCGTATTA TCGTGGCGGA CTCCCGCAAC 
CATCGCATTC AGGTGTTCGA ACCCAACGGC AACTTTTTAT GCAAGTTTGG GACTCACGGT 
AATGGCTTCG GGCAAATGGA TCGCCCCTCT GGTATAGCAG TGACCCCAGA TGGAGTGATT 
GTGGCCGTGG ATTTCGGAAA CAACCGAATC CTCATGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The heterochronic genes lin-28a and lin-28b play an essential and evolutionarily conserved role in early zebrafish development.
PloS one9(2)e88086(2014)
Ouchi Y,Yamamoto J,Iwamoto T

Origin and evolution of TRIM proteins: new insights from the complete TRIM repertoire of zebrafish and pufferfish.
PloS one6(7)e22022(2011)
Boudinot P,van der Aa LM,Jouneau L,Du Pasquier L,Pontarotti P,Briolat V,Benmansour A,Levraud JP

Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs.
Nucleic acids research37(10)e77(2009 Jun)
Hsu RJ,Yang HJ,Tsai HJ