col11a2 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009294049.2	XP_009292324.1	collagen alpha-2(XI) chain isoform X7
NM_001079992.2	NP_001073461.2	collagen alpha-2(XI) chain isoform 1
NM_001328265.1	NP_001315194.1	collagen alpha-2(XI) chain isoform 2
XM_017352252.1	XP_017207741.1	collagen alpha-2(XI) chain isoform X2
XM_005159443.3	XP_005159500.1	collagen alpha-2(XI) chain isoform X1
XM_017352253.1	XP_017207742.1	collagen alpha-2(XI) chain isoform X6
XM_005159445.4	XP_005159502.1	collagen alpha-2(XI) chain isoform X3
XM_017352253.2	XP_017207742.1	collagen alpha-2(XI) chain isoform X4
XM_005159448.4	XP_005159505.1	collagen alpha-2(XI) chain isoform X5
XM_005159443.2	XP_005159500.1	collagen alpha-2(XI) chain isoform X1
XM_005159445.3	XP_005159502.1	collagen alpha-2(XI) chain isoform X3
XM_005159448.3	XP_005159505.1	collagen alpha-2(XI) chain isoform X4
XM_017352252.2	XP_017207741.1	collagen alpha-2(XI) chain isoform X2
XM_009294049.3	XP_009292324.1	collagen alpha-2(XI) chain isoform X7
XM_005159442.3	XP_005159499.1	collagen alpha-2(XI) chain isoform X1
XM_005159450.3	XP_005159507.1	collagen alpha-2(XI) chain isoform X5
XM_005159442.4	XP_005159499.1	collagen alpha-2(XI) chain isoform X1
XM_005159450.4	XP_005159507.1	collagen alpha-2(XI) chain isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23892	NM_001328265.1 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa43572	NM_001079992.2 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa23891	XM_005159445.4 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa23892	XM_017352253.2 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa23893	XM_005159448.4 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa23895	XM_005159450.4 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa43572	XM_009294049.3 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa56071	XM_017352252.2 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa23889	XM_005159442.4 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa23889	XM_005159443.3 Latest version!	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODa23892	XM_017352253.1	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa23889	XM_005159443.2	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa23891	XM_005159445.3	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa23893	XM_005159448.3	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa56071	XM_017352252.1	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa43572	XM_009294049.2	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa23889	XM_005159442.3	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa23895	XM_005159450.3	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

col11a2 ( NM_001328265.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315194.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa23892
Clone ID Related Accession (Same CDS sequence)	NM_001328265.1 , XM_017352253.1 , XM_017352253.2
Accession Version	NM_001328265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP017221.4 and FP085392.6. On Jun 14, 2016 this sequence version replaced XM_005159447.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ485666.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCACTTTGAT GAAGAGCAGG ATGATTATGA ATACCCCTAT 
TATTATGAAG GAACTACCAG TGTCCCTCCC TCCCCATCCA AACCTCCCTC CGTCCCAGCC 
GAGACACTCA CTGAACAGGA CGTAGATCCT GCTTACTCAG AGGCTGACCA CACCCCCACA 
GAGACCGATT ATTATTACAC AGTAGAAGAA GAGGCGGCTC CACAGCCAGA GAGTGATCTG 
ACTGGATATG ACGAAAGCAT TAACCAGGTC GACGAGACTG TCCCCGTTGA GGAAATGGAT 
GCTACCAAGA CAGGTGGTGA GGTGGCGTCT CAAACGCCAG AAGAGCCCTT CACTGAGGAG 
TACATTACAA GCGACTTTGG GATGAAGGAG TATGACTATT CCTACAAGGA CTACAATGAG 
CCCATCATTG AGAGCAAGAC GGAGGACACT CTAGGCCCTG CCCTGTCCGC TGTGACAGAG 
GAGGGGGGTG CCTCAGTGCG AGCCATTAAG GGAGAGAAGG GAGAGCCTGC TGTCCTTGAA 
CCTGGCATGC TGATTGAAGG ACCACCAGGA CCAGAAGGAC CAGCAGGTCT CCCTGGCCCC 
TCTGGCCCCC AAGGACAACC TGGTTCTGTT GGTGATCCTG GTGAGAGGGG TCCTACTGGT 
AAGGCTGGTC TACCTGGAGC AGATGGTGTT CCAGGACCTC CAGGAACCTC TGTCATGCTT 
CCTTTCCGCT TTGGACAGAG CGCTGGAGAG AAAGGACCTG TTGCCTCTGC ACAGGAAGCC 
CAAGCTCAAG CCATTCTGTC TCAAGCTCGG CTTGCCCTGA AAGGACCATC CGGGCCAATG 
GGTTTCACTG GCCGTCCTGG ACCTTTGGGA ACACCTGGAA GTCCTGGCTT TAAGGGAGAA 
AGGGGGGACC CTGGTGCTCA GGGTCCTAGA GGTCCTCAGG GTGTGTCTGG TCCTCCTGGC 
AAAGCTGGTC GGAGGGGTCG CGCTGGTGCT GATGGAGCCA GAGGGATGCC AGGAGAGTCT 
GGAATTAAGG GTGACCGTGG TTTTGATGGC CTTCCTGGTT TGCCTGGTGA CAAGGGTTAT 
CGGGGTCAAC CTGGAGGAAT GGGACCCCCA GGACCACATG GAGAGGACGG AGAGAGGGGA 
GATGATGGAG ATGTTGGACC CAGAGGTCTT CCTGGCGAAC CTGGACCCCG TGGTTTGTTG 
GGGCCTAAAG GGCCTTCTGG ACTTCCTGGA CCTCCTGGTG TTCGTGGAAA TGATGGTCCA 
CATGGTCCAA AAGGAAACTT AGGACCACAA GGAGAGCCCG GTCCTCCAGG CCAGCAGGGG 
GCTCCAGGAA CACAGGGAAT GCCAGGTCCA CAGGGTGCCA CTGGACCACC AGGAGAGAAA 
GGACCCACAG GAAAGCCAGG TCTACCAGGA ATGCCAGGAG CTGATGGTCC TCCCGGTCAT 
CCCGGGAAGG AAGGTCCTGG TGGAACCAAA GGAAATCAAG GTCCTAATGG TCCTCAGGGC 
TCTATTGGCT ATCCTGGCCC TCGGGGATTG AAGGGAGCTC AAGGTATCCG TGGACTGAAA 
GGTCATAAAG GAGAGAAGGG AGAGGATGGA TTCCCAGGAA TTAAAGGAGA CTTTGGTGTG 
AAGGGAGAGA GGGGAGAGGT GGGCGTGCCC GGCCCAAGAG GAGAAGATGG TCCTGAGGGG 
CCAAAGGGTC GCGTCGGACC CCCTGGTGAA ATTGGTCCCC TCGGCCTGTT GGGAGAGAAG 
GGTAAACTTG GTGTTCCTGG ACTTCCTGGA TATCCTGGAC GTCAAGGAAT AAAGGGGTCC 
CTTGGTTTCC CAGGATTCCC TGGTACAAAT GGCGAGAAGG GCACAAGGGG TTTGATTGGA 
AAACCTGGAC CAAGAGGACA AAGAGGGCCG ACAGGTCCTA GAGGGCAGAG AGGACCACGT 
GGGGCAACTG GGAAATCAGG TGCTAAGGGT GGATCAGGAA GTGACGGGCC TCCTGGACCA 
CCAGGAGAGA GAGGGCTAAC TGGACCTCAG GGTGCTAATG GATTCCCTGG GCCAAAGGGA 
CCTCCCGGAC CTCCAGGGAA AGATGGACTT CCTGGACACC CTGGACAGAG AGGCGAAGTC 
GGTTTCCAAG GCAAAGTTGG ACCACCTGGC CCACCTGGAG TTGTTGGCCC ACACGGTCCC 
TCTGGTGAGA CTGGTCAGAT GGGTGAGCGT GGCCACCCTG GACCTCCAGG ACCACCTGGT 
GAGCAAGGCC TTTCTGGCTC CTCTGGTAAA GAAGGAACCA AAGGAGATCC CGGTCCTCCT 
GGTGGCCCCG GTAAAGATGG ACCCCCTGGA CTTAGAGGAT TCCCAGGAGA GAGAGGACTA 
CCAGGAACTC CAGGTTCTGG AGGACTGAAA GGAAATGAAG GCCCTGCTGG ACCACCTGGT 
CCTGCGGGTT CATCTGGGGA GAGAGGTGGT GCTGGCACTG CAGGCCCTGT CGGCCCACCT 
GGACGACCTG GACCTCAGGG ACCCCCTGGA ACTTCAGGTG AAAAGGGAGT ACCAGGCGAG 
AAAGGTCCGG TTGGTCCAGC TGGTCGTGAT GGCATCCAAG GCCCAGTTGG TCTCCCAGGC 
CCTGCCGGAC CTCCAGGTAT TTCTGGAGAA GATGGAGACA AGGGTGAGGT TGGAGAGCCA 
GGACAAAAGG GAGCTAAAGG GTCCAAAGGA GAACATGGTC CTCCTGGTCC ACCTGGGCCG 
ATGGGTCCTG TTGGACAGCC TGGTGCAGCG GGTGCTGATG GTGAGACGGG TCCAAGAGGT 
CAACAGGGAC AGTTTGGTGC CAAGGGAGAT GAAGGCACAA GAGGTTTCCC TGGTCCCCCT 
GGCCCCATAG GATTGCAGGG TTTGCCAGGC CCAGGTGGAG AAAAGGGAGA GACAGGAGAT 
GTTGGACCCT TGGGTCCTCC TGGTCCTCCA GGGCCGCGTG GTCCTGCTGG TCCTAATGGT 
GCTGATGGTC CCCAAGGTCC TCCAGGAGGT TTGGGTAATC CAGGTCCCAT AGGAGAGAAG 
GGTGAGCCTG GTGAATCTGG ACCACCTGGT GTTGGTGGAG AGCCAGGAAA ATTGGGCCCA 
AGAGGAGAGC GTGGTGAGAA GGGAGAGTCC GGGCAGCCTG GCACCCCTGG TCCTCCTGGA 
GGAAAGGGAC CCACTGGAGA TGACGGACCC AAAGGAAACC CTGGTCCTGT TGGATTCCCT 
GGTGATCCTG GACCCCCTGG TGAAGTTGGA CCACGAGGTC AAGATGGTGC AAAGGGTGAC 
AGAGGAGAGG ATGGAGAACA AGGAGAAGCC GGCTCACCTG GACCTACTGG AGAGAACGGA 
CCACCTGGAC CTCCAGGAAA GAGAGGTCCT GCAGGCACAA GGGGACCGGA GGGCCGTCAA 
GGAGAGAAAG GAAGCAAGGG TGATTCAGGT GCTCTTGGTC CTCCCGGAAA AACTGGCCCT 
GTGGGGCCAC AGGGACAACC CGGAAAACCA GGAACTGAGG GTCTAAGAGG GTTGCCTGGA 
ACAGTGGGTG AGCAAGGTTC TCCAGGTGCT GCAGGCCCTA AAGGACCTCC TGGACCTCTG 
GGTCCTCCAG GTCTGGCTGG TCTTCGCGGT GATCCCGGTG CTAAAGGTGA GAAGGGACAT 
CCAGGTATGC TCGGACTGAT CGGGCCTGCT GGAGAACAAG GAGAGAAGGG AGACAGAGGC 
ATGCCAGGAC CACAGGGATC AACAGGACCC AAGGGAGAAA CGGGTATATC TGGAGGAACT 
GGTCCACTTG GTCCTGCAGG TCCTGCTGGT ATGCCTGGCC CGAGGGGTGT CAAAGGAGCT 
AAAGGTGCCA GTGGTGGGGC TGGTCCTAAA GGAGAAAAGG GTGTACAAGG ACCACCAGGA 
CCACCTGGTC CCCCTGGTGA TGTCATCCAG CCGCTGCCCA TTCAGATCCC CAAGAAGTCC 
AAGCGCTCCA TCGACGCCAG TAAGATTCTG TCTGAGGCTG ACGCTGAGGC TGCCGACACC 
ACTGGCTCTG AGTTCCTGAC TGGTAATGAA GGAATGGAGG AGATCTTCGG CTCACTTAAT 
TCCCTGCGCC AGGAGATCGA GAGCATGCGC TTCCCATTGG GCACAAAGGA GAGCCCCGGC 
CGCACCTGCC AGGATCTCCA TCTCAGCCAA CCAGATCTGA AAGACGGCGA GTATTGGATT 
GATCCAAATC AAGGCTGTGC TCGCGACTCT TTCAAGGTTT ACTGTAACTT CACTGCCGGC 
GGAGAGACCT GCCTGTATCC CAGCAAAGCA GTGGAGAATG TAAAGATGAA CTCTTGGATA 
AAGGAGAAGC CTGGGTCCTT GTTCAGTCAG TTTGCAAAGG GCAACAAGTT TTCATATGTA 
GACTCGGTTG GAGAGGCTGT GGGTGTTGTT CAGCTGGGCT TCTTGCGGCT GCTCAGTGTC 
CAGGCCAGAC AGAACCTCAC CTACCACTGC CAACGCTCGG TGGCCTGGAC TGACCAAACC 
ACCGACGGCG GATACAAGCG GGCGCTGCGT CTGCAGGGGG CCAATGATGA GGAGATCAGC 
TATGAGACTA GCCCATATAT CAAAGCCCTC ATCGACGGCT GCTCCTATCG TAAGGGTCTG 
GACAGGACGG TGCTGGAGGT GAACACACCT CAGGTGGAGC AGCTCCCTCT GCTGGACATC 
AGGATCTCAG ACTTTGGGGA GGACAATCAG AAATTTGGTT TTGAAGTAGG ACCTGTCTGT 
TTCCTGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315194.1
CDS	317..5728
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYHFDEEQDDYEYPYYYEGTTSVPPSPSKPPSVPAETLTEQDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	NM_001328265.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328265.1

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCACTTTGAT GAAGAGCAGG ATGATTATGA ATACCCCTAT 
TATTATGAAG GAACTACCAG TGTCCCTCCC TCCCCATCCA AACCTCCCTC CGTCCCAGCC 
GAGACACTCA CTGAACAGGA CGTAGATCCT GCTTACTCAG AGGCTGACCA CACCCCCACA 
GAGACCGATT ATTATTACAC AGTAGAAGAA GAGGCGGCTC CACAGCCAGA GAGTGATCTG 
ACTGGATATG ACGAAAGCAT TAACCAGGTC GACGAGACTG TCCCCGTTGA GGAAATGGAT 
GCTACCAAGA CAGGTGGTGA GGTGGCGTCT CAAACGCCAG AAGAGCCCTT CACTGAGGAG 
TACATTACAA GCGACTTTGG GATGAAGGAG TATGACTATT CCTACAAGGA CTACAATGAG 
CCCATCATTG AGAGCAAGAC GGAGGACACT CTAGGCCCTG CCCTGTCCGC TGTGACAGAG 
GAGGGGGGTG CCTCAGTGCG AGCCATTAAG GGAGAGAAGG GAGAGCCTGC TGTCCTTGAA 
CCTGGCATGC TGATTGAAGG ACCACCAGGA CCAGAAGGAC CAGCAGGTCT CCCTGGCCCC 
TCTGGCCCCC AAGGACAACC TGGTTCTGTT GGTGATCCTG GTGAGAGGGG TCCTACTGGT 
AAGGCTGGTC TACCTGGAGC AGATGGTGTT CCAGGACCTC CAGGAACCTC TGTCATGCTT 
CCTTTCCGCT TTGGACAGAG CGCTGGAGAG AAAGGACCTG TTGCCTCTGC ACAGGAAGCC 
CAAGCTCAAG CCATTCTGTC TCAAGCTCGG CTTGCCCTGA AAGGACCATC CGGGCCAATG 
GGTTTCACTG GCCGTCCTGG ACCTTTGGGA ACACCTGGAA GTCCTGGCTT TAAGGGAGAA 
AGGGGGGACC CTGGTGCTCA GGGTCCTAGA GGTCCTCAGG GTGTGTCTGG TCCTCCTGGC 
AAAGCTGGTC GGAGGGGTCG CGCTGGTGCT GATGGAGCCA GAGGGATGCC AGGAGAGTCT 
GGAATTAAGG GTGACCGTGG TTTTGATGGC CTTCCTGGTT TGCCTGGTGA CAAGGGTTAT 
CGGGGTCAAC CTGGAGGAAT GGGACCCCCA GGACCACATG GAGAGGACGG AGAGAGGGGA 
GATGATGGAG ATGTTGGACC CAGAGGTCTT CCTGGCGAAC CTGGACCCCG TGGTTTGTTG 
GGGCCTAAAG GGCCTTCTGG ACTTCCTGGA CCTCCTGGTG TTCGTGGAAA TGATGGTCCA 
CATGGTCCAA AAGGAAACTT AGGACCACAA GGAGAGCCCG GTCCTCCAGG CCAGCAGGGG 
GCTCCAGGAA CACAGGGAAT GCCAGGTCCA CAGGGTGCCA CTGGACCACC AGGAGAGAAA 
GGACCCACAG GAAAGCCAGG TCTACCAGGA ATGCCAGGAG CTGATGGTCC TCCCGGTCAT 
CCCGGGAAGG AAGGTCCTGG TGGAACCAAA GGAAATCAAG GTCCTAATGG TCCTCAGGGC 
TCTATTGGCT ATCCTGGCCC TCGGGGATTG AAGGGAGCTC AAGGTATCCG TGGACTGAAA 
GGTCATAAAG GAGAGAAGGG AGAGGATGGA TTCCCAGGAA TTAAAGGAGA CTTTGGTGTG 
AAGGGAGAGA GGGGAGAGGT GGGCGTGCCC GGCCCAAGAG GAGAAGATGG TCCTGAGGGG 
CCAAAGGGTC GCGTCGGACC CCCTGGTGAA ATTGGTCCCC TCGGCCTGTT GGGAGAGAAG 
GGTAAACTTG GTGTTCCTGG ACTTCCTGGA TATCCTGGAC GTCAAGGAAT AAAGGGGTCC 
CTTGGTTTCC CAGGATTCCC TGGTACAAAT GGCGAGAAGG GCACAAGGGG TTTGATTGGA 
AAACCTGGAC CAAGAGGACA AAGAGGGCCG ACAGGTCCTA GAGGGCAGAG AGGACCACGT 
GGGGCAACTG GGAAATCAGG TGCTAAGGGT GGATCAGGAA GTGACGGGCC TCCTGGACCA 
CCAGGAGAGA GAGGGCTAAC TGGACCTCAG GGTGCTAATG GATTCCCTGG GCCAAAGGGA 
CCTCCCGGAC CTCCAGGGAA AGATGGACTT CCTGGACACC CTGGACAGAG AGGCGAAGTC 
GGTTTCCAAG GCAAAGTTGG ACCACCTGGC CCACCTGGAG TTGTTGGCCC ACACGGTCCC 
TCTGGTGAGA CTGGTCAGAT GGGTGAGCGT GGCCACCCTG GACCTCCAGG ACCACCTGGT 
GAGCAAGGCC TTTCTGGCTC CTCTGGTAAA GAAGGAACCA AAGGAGATCC CGGTCCTCCT 
GGTGGCCCCG GTAAAGATGG ACCCCCTGGA CTTAGAGGAT TCCCAGGAGA GAGAGGACTA 
CCAGGAACTC CAGGTTCTGG AGGACTGAAA GGAAATGAAG GCCCTGCTGG ACCACCTGGT 
CCTGCGGGTT CATCTGGGGA GAGAGGTGGT GCTGGCACTG CAGGCCCTGT CGGCCCACCT 
GGACGACCTG GACCTCAGGG ACCCCCTGGA ACTTCAGGTG AAAAGGGAGT ACCAGGCGAG 
AAAGGTCCGG TTGGTCCAGC TGGTCGTGAT GGCATCCAAG GCCCAGTTGG TCTCCCAGGC 
CCTGCCGGAC CTCCAGGTAT TTCTGGAGAA GATGGAGACA AGGGTGAGGT TGGAGAGCCA 
GGACAAAAGG GAGCTAAAGG GTCCAAAGGA GAACATGGTC CTCCTGGTCC ACCTGGGCCG 
ATGGGTCCTG TTGGACAGCC TGGTGCAGCG GGTGCTGATG GTGAGACGGG TCCAAGAGGT 
CAACAGGGAC AGTTTGGTGC CAAGGGAGAT GAAGGCACAA GAGGTTTCCC TGGTCCCCCT 
GGCCCCATAG GATTGCAGGG TTTGCCAGGC CCAGGTGGAG AAAAGGGAGA GACAGGAGAT 
GTTGGACCCT TGGGTCCTCC TGGTCCTCCA GGGCCGCGTG GTCCTGCTGG TCCTAATGGT 
GCTGATGGTC CCCAAGGTCC TCCAGGAGGT TTGGGTAATC CAGGTCCCAT AGGAGAGAAG 
GGTGAGCCTG GTGAATCTGG ACCACCTGGT GTTGGTGGAG AGCCAGGAAA ATTGGGCCCA 
AGAGGAGAGC GTGGTGAGAA GGGAGAGTCC GGGCAGCCTG GCACCCCTGG TCCTCCTGGA 
GGAAAGGGAC CCACTGGAGA TGACGGACCC AAAGGAAACC CTGGTCCTGT TGGATTCCCT 
GGTGATCCTG GACCCCCTGG TGAAGTTGGA CCACGAGGTC AAGATGGTGC AAAGGGTGAC 
AGAGGAGAGG ATGGAGAACA AGGAGAAGCC GGCTCACCTG GACCTACTGG AGAGAACGGA 
CCACCTGGAC CTCCAGGAAA GAGAGGTCCT GCAGGCACAA GGGGACCGGA GGGCCGTCAA 
GGAGAGAAAG GAAGCAAGGG TGATTCAGGT GCTCTTGGTC CTCCCGGAAA AACTGGCCCT 
GTGGGGCCAC AGGGACAACC CGGAAAACCA GGAACTGAGG GTCTAAGAGG GTTGCCTGGA 
ACAGTGGGTG AGCAAGGTTC TCCAGGTGCT GCAGGCCCTA AAGGACCTCC TGGACCTCTG 
GGTCCTCCAG GTCTGGCTGG TCTTCGCGGT GATCCCGGTG CTAAAGGTGA GAAGGGACAT 
CCAGGTATGC TCGGACTGAT CGGGCCTGCT GGAGAACAAG GAGAGAAGGG AGACAGAGGC 
ATGCCAGGAC CACAGGGATC AACAGGACCC AAGGGAGAAA CGGGTATATC TGGAGGAACT 
GGTCCACTTG GTCCTGCAGG TCCTGCTGGT ATGCCTGGCC CGAGGGGTGT CAAAGGAGCT 
AAAGGTGCCA GTGGTGGGGC TGGTCCTAAA GGAGAAAAGG GTGTACAAGG ACCACCAGGA 
CCACCTGGTC CCCCTGGTGA TGTCATCCAG CCGCTGCCCA TTCAGATCCC CAAGAAGTCC 
AAGCGCTCCA TCGACGCCAG TAAGATTCTG TCTGAGGCTG ACGCTGAGGC TGCCGACACC 
ACTGGCTCTG AGTTCCTGAC TGGTAATGAA GGAATGGAGG AGATCTTCGG CTCACTTAAT 
TCCCTGCGCC AGGAGATCGA GAGCATGCGC TTCCCATTGG GCACAAAGGA GAGCCCCGGC 
CGCACCTGCC AGGATCTCCA TCTCAGCCAA CCAGATCTGA AAGACGGCGA GTATTGGATT 
GATCCAAATC AAGGCTGTGC TCGCGACTCT TTCAAGGTTT ACTGTAACTT CACTGCCGGC 
GGAGAGACCT GCCTGTATCC CAGCAAAGCA GTGGAGAATG TAAAGATGAA CTCTTGGATA 
AAGGAGAAGC CTGGGTCCTT GTTCAGTCAG TTTGCAAAGG GCAACAAGTT TTCATATGTA 
GACTCGGTTG GAGAGGCTGT GGGTGTTGTT CAGCTGGGCT TCTTGCGGCT GCTCAGTGTC 
CAGGCCAGAC AGAACCTCAC CTACCACTGC CAACGCTCGG TGGCCTGGAC TGACCAAACC 
ACCGACGGCG GATACAAGCG GGCGCTGCGT CTGCAGGGGG CCAATGATGA GGAGATCAGC 
TATGAGACTA GCCCATATAT CAAAGCCCTC ATCGACGGCT GCTCCTATCG TAAGGGTCTG 
GACAGGACGG TGCTGGAGGT GAACACACCT CAGGTGGAGC AGCTCCCTCT GCTGGACATC 
AGGATCTCAG ACTTTGGGGA GGACAATCAG AAATTTGGTT TTGAAGTAGG ACCTGTCTGT 
TTCCTGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( NM_001079992.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073461.2 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant 1, mRNA.

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CloneID	ODa43572
Clone ID Related Accession (Same CDS sequence)	XM_009294049.2 , NM_001079992.2 , XM_009294049.3
Accession Version	NM_001079992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP017221.4 and FP085392.6. On Jun 10, 2016 this sequence version replaced NM_001079992.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AM287214.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCGCAGGTCA AAGCACAACC AACCAAAAGC AAGACAGACA 
AAACGAAAGC CAATGCGTCC CCCGCAAAAC CAAAAGACAC CAAAGCCAAA GCAGCCTCCG 
TCACTCAAAA ACCTGCTAAA TTTCCCACCC TTCGACAAGG CTGTTGCCTC AGTGCGAGCC 
ATTAAGGGAG AGAAGGGAGA GCCTGCTGTC CTTGAACCTG GCATGCTGAT TGAAGGACCA 
CCAGGACCAG AAGGACCAGC AGGTCTCCCT GGCCCCTCTG GCCCCCAAGG ACAACCTGGT 
TCTGTTGGTG ATCCTGGTGA GAGGGGTCCT ACTGGTAAGG CTGGTCTACC TGGAGCAGAT 
GGTGTTCCAG GACCTCCAGG AACCTCTGTC ATGCTTCCTT TCCGCTTTGG ACAGAGCGCT 
GGAGAGAAAG GACCTGTTGC CTCTGCACAG GAAGCCCAAG CTCAAGCCAT TCTGTCTCAA 
GCTCGGCTTG CCCTGAAAGG ACCATCCGGG CCAATGGGTT TCACTGGCCG TCCTGGACCT 
TTGGGAACAC CTGGAAGTCC TGGCTTTAAG GGAGAAAGGG GGGACCCTGG TGCTCAGGGT 
CCTAGAGGTC CTCAGGGTGT GTCTGGTCCT CCTGGCAAAG CTGGTCGGAG GGGTCGCGCT 
GGTGCTGATG GAGCCAGAGG GATGCCAGGA GAGTCTGGAA TTAAGGGTGA CCGTGGTTTT 
GATGGCCTTC CTGGTTTGCC TGGTGACAAG GGTTATCGGG GTCAACCTGG AGGAATGGGA 
CCCCCAGGAC CACATGGAGA GGACGGAGAG AGGGGAGATG ATGGAGATGT TGGACCCAGA 
GGTCTTCCTG GCGAACCTGG ACCCCGTGGT TTGTTGGGGC CTAAAGGGCC TTCTGGACTT 
CCTGGACCTC CTGGTGTTCG TGGAAATGAT GGTCCACATG GTCCAAAAGG AAACTTAGGA 
CCACAAGGAG AGCCCGGTCC TCCAGGCCAG CAGGGGGCTC CAGGAACACA GGGAATGCCA 
GGTCCACAGG GTGCCACTGG ACCACCAGGA GAGAAAGGAC CCACAGGAAA GCCAGGTCTA 
CCAGGAATGC CAGGAGCTGA TGGTCCTCCC GGTCATCCCG GGAAGGAAGG TCCTGGTGGA 
ACCAAAGGAA ATCAAGGTCC TAATGGTCCT CAGGGCTCTA TTGGCTATCC TGGCCCTCGG 
GGATTGAAGG GAGCTCAAGG TATCCGTGGA CTGAAAGGTC ATAAAGGAGA GAAGGGAGAG 
GATGGATTCC CAGGAATTAA AGGAGACTTT GGTGTGAAGG GAGAGAGGGG AGAGGTGGGC 
GTGCCCGGCC CAAGAGGAGA AGATGGTCCT GAGGGGCCAA AGGGTCGCGT CGGACCCCCT 
GGTGAAATTG GTCCCCTCGG CCTGTTGGGA GAGAAGGGTA AACTTGGTGT TCCTGGACTT 
CCTGGATATC CTGGACGTCA AGGAATAAAG GGGTCCCTTG GTTTCCCAGG ATTCCCTGGT 
ACAAATGGCG AGAAGGGCAC AAGGGGTTTG ATTGGAAAAC CTGGACCAAG AGGACAAAGA 
GGGCCGACAG GTCCTAGAGG GCAGAGAGGA CCACGTGGGG CAACTGGGAA ATCAGGTGCT 
AAGGGTGGAT CAGGAAGTGA CGGGCCTCCT GGACCACCAG GAGAGAGAGG GCTAACTGGA 
CCTCAGGGTG CTAATGGATT CCCTGGGCCA AAGGGACCTC CCGGACCTCC AGGGAAAGAT 
GGACTTCCTG GACACCCTGG ACAGAGAGGC GAAGTCGGTT TCCAAGGCAA AGTTGGACCA 
CCTGGCCCAC CTGGAGTTGT TGGCCCACAC GGTCCCTCTG GTGAGACTGG TCAGATGGGT 
GAGCGTGGCC ACCCTGGACC TCCAGGACCA CCTGGTGAGC AAGGCCTTTC TGGCTCCTCT 
GGTAAAGAAG GAACCAAAGG AGATCCCGGT CCTCCTGGTG GCCCCGGTAA AGATGGACCC 
CCTGGACTTA GAGGATTCCC AGGAGAGAGA GGACTACCAG GAACTCCAGG TTCTGGAGGA 
CTGAAAGGAA ATGAAGGCCC TGCTGGACCA CCTGGTCCTG CGGGTTCATC TGGGGAGAGA 
GGTGGTGCTG GCACTGCAGG CCCTGTCGGC CCACCTGGAC GACCTGGACC TCAGGGACCC 
CCTGGAACTT CAGGTGAAAA GGGAGTACCA GGCGAGAAAG GTCCGGTTGG TCCAGCTGGT 
CGTGATGGCA TCCAAGGCCC AGTTGGTCTC CCAGGCCCTG CCGGACCTCC AGGTATTTCT 
GGAGAAGATG GAGACAAGGG TGAGGTTGGA GAGCCAGGAC AAAAGGGAGC TAAAGGGTCC 
AAAGGAGAAC ATGGTCCTCC TGGTCCACCT GGGCCGATGG GTCCTGTTGG ACAGCCTGGT 
GCAGCGGGTG CTGATGGTGA GACGGGTCCA AGAGGTCAAC AGGGACAGTT TGGTGCCAAG 
GGAGATGAAG GCACAAGAGG TTTCCCTGGT CCCCCTGGCC CCATAGGATT GCAGGGTTTG 
CCAGGCCCAG GTGGAGAAAA GGGAGAGACA GGAGATGTTG GACCCTTGGG TCCTCCTGGT 
CCTCCAGGGC CGCGTGGTCC TGCTGGTCCT AATGGTGCTG ATGGTCCCCA AGGTCCTCCA 
GGAGGTTTGG GTAATCCAGG TCCCATAGGA GAGAAGGGTG AGCCTGGTGA ATCTGGACCA 
CCTGGTGTTG GTGGAGAGCC AGGAAAATTG GGCCCAAGAG GAGAGCGTGG TGAGAAGGGA 
GAGTCCGGGC AGCCTGGCAC CCCTGGTCCT CCTGGAGGAA AGGGACCCAC TGGAGATGAC 
GGACCCAAAG GAAACCCTGG TCCTGTTGGA TTCCCTGGTG ATCCTGGACC CCCTGGTGAA 
GTTGGACCAC GAGGTCAAGA TGGTGCAAAG GGTGACAGAG GAGAGGATGG AGAACAAGGA 
GAAGCCGGCT CACCTGGACC TACTGGAGAG AACGGACCAC CTGGACCTCC AGGAAAGAGA 
GGTCCTGCAG GCACAAGGGG ACCGGAGGGC CGTCAAGGAG AGAAAGGAAG CAAGGGTGAT 
TCAGGTGCTC TTGGTCCTCC CGGAAAAACT GGCCCTGTGG GGCCACAGGG ACAACCCGGA 
AAACCAGGAA CTGAGGGTCT AAGAGGGTTG CCTGGAACAG TGGGTGAGCA AGGTTCTCCA 
GGTGCTGCAG GCCCTAAAGG ACCTCCTGGA CCTCTGGGTC CTCCAGGTCT GGCTGGTCTT 
CGCGGTGATC CCGGTGCTAA AGGTGAGAAG GGACATCCAG GTATGCTCGG ACTGATCGGG 
CCTGCTGGAG AACAAGGAGA GAAGGGAGAC AGAGGCATGC CAGGACCACA GGGATCAACA 
GGACCCAAGG GAGAAACGGG TATATCTGGA GGAACTGGTC CACTTGGTCC TGCAGGTCCT 
GCTGGTATGC CTGGCCCGAG GGGTGTCAAA GGAGCTAAAG GTGCCAGTGG TGGGGCTGGT 
CCTAAAGGAG AAAAGGGTGT ACAAGGACCA CCAGGACCAC CTGGTCCCCC TGGTGATGTC 
ATCCAGCCGC TGCCCATTCA GATCCCCAAG AAGTCCAAGC GCTCCATCGA CGCCAGTAAG 
ATTCTGTCTG AGGCTGACGC TGAGGCTGCC GACACCACTG GCTCTGAGTT CCTGACTGGT 
AATGAAGGAA TGGAGGAGAT CTTCGGCTCA CTTAATTCCC TGCGCCAGGA GATCGAGAGC 
ATGCGCTTCC CATTGGGCAC AAAGGAGAGC CCCGGCCGCA CCTGCCAGGA TCTCCATCTC 
AGCCAACCAG ATCTGAAAGA CGGCGAGTAT TGGATTGATC CAAATCAAGG CTGTGCTCGC 
GACTCTTTCA AGGTTTACTG TAACTTCACT GCCGGCGGAG AGACCTGCCT GTATCCCAGC 
AAAGCAGTGG AGAATGTAAA GATGAACTCT TGGATAAAGG AGAAGCCTGG GTCCTTGTTC 
AGTCAGTTTG CAAAGGGCAA CAAGTTTTCA TATGTAGACT CGGTTGGAGA GGCTGTGGGT 
GTTGTTCAGC TGGGCTTCTT GCGGCTGCTC AGTGTCCAGG CCAGACAGAA CCTCACCTAC 
CACTGCCAAC GCTCGGTGGC CTGGACTGAC CAAACCACCG ACGGCGGATA CAAGCGGGCG 
CTGCGTCTGC AGGGGGCCAA TGATGAGGAG ATCAGCTATG AGACTAGCCC ATATATCAAA 
GCCCTCATCG ACGGCTGCTC CTATCGTAAG GGTCTGGACA GGACGGTGCT GGAGGTGAAC 
ACACCTCAGG TGGAGCAGCT CCCTCTGCTG GACATCAGGA TCTCAGACTT TGGGGAGGAC 
AATCAGAAAT TTGGTTTTGA AGTAGGACCT GTCTGTTTCC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073461.2
CDS	317..5404
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYRRSKHNQPKARQTKRKPMRPPQNQKTPKPKQPPSLKNLLNFPPFDKAVASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	NM_001079992.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079992.2

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCGCAGGTCA AAGCACAACC AACCAAAAGC AAGACAGACA 
AAACGAAAGC CAATGCGTCC CCCGCAAAAC CAAAAGACAC CAAAGCCAAA GCAGCCTCCG 
TCACTCAAAA ACCTGCTAAA TTTCCCACCC TTCGACAAGG CTGTTGCCTC AGTGCGAGCC 
ATTAAGGGAG AGAAGGGAGA GCCTGCTGTC CTTGAACCTG GCATGCTGAT TGAAGGACCA 
CCAGGACCAG AAGGACCAGC AGGTCTCCCT GGCCCCTCTG GCCCCCAAGG ACAACCTGGT 
TCTGTTGGTG ATCCTGGTGA GAGGGGTCCT ACTGGTAAGG CTGGTCTACC TGGAGCAGAT 
GGTGTTCCAG GACCTCCAGG AACCTCTGTC ATGCTTCCTT TCCGCTTTGG ACAGAGCGCT 
GGAGAGAAAG GACCTGTTGC CTCTGCACAG GAAGCCCAAG CTCAAGCCAT TCTGTCTCAA 
GCTCGGCTTG CCCTGAAAGG ACCATCCGGG CCAATGGGTT TCACTGGCCG TCCTGGACCT 
TTGGGAACAC CTGGAAGTCC TGGCTTTAAG GGAGAAAGGG GGGACCCTGG TGCTCAGGGT 
CCTAGAGGTC CTCAGGGTGT GTCTGGTCCT CCTGGCAAAG CTGGTCGGAG GGGTCGCGCT 
GGTGCTGATG GAGCCAGAGG GATGCCAGGA GAGTCTGGAA TTAAGGGTGA CCGTGGTTTT 
GATGGCCTTC CTGGTTTGCC TGGTGACAAG GGTTATCGGG GTCAACCTGG AGGAATGGGA 
CCCCCAGGAC CACATGGAGA GGACGGAGAG AGGGGAGATG ATGGAGATGT TGGACCCAGA 
GGTCTTCCTG GCGAACCTGG ACCCCGTGGT TTGTTGGGGC CTAAAGGGCC TTCTGGACTT 
CCTGGACCTC CTGGTGTTCG TGGAAATGAT GGTCCACATG GTCCAAAAGG AAACTTAGGA 
CCACAAGGAG AGCCCGGTCC TCCAGGCCAG CAGGGGGCTC CAGGAACACA GGGAATGCCA 
GGTCCACAGG GTGCCACTGG ACCACCAGGA GAGAAAGGAC CCACAGGAAA GCCAGGTCTA 
CCAGGAATGC CAGGAGCTGA TGGTCCTCCC GGTCATCCCG GGAAGGAAGG TCCTGGTGGA 
ACCAAAGGAA ATCAAGGTCC TAATGGTCCT CAGGGCTCTA TTGGCTATCC TGGCCCTCGG 
GGATTGAAGG GAGCTCAAGG TATCCGTGGA CTGAAAGGTC ATAAAGGAGA GAAGGGAGAG 
GATGGATTCC CAGGAATTAA AGGAGACTTT GGTGTGAAGG GAGAGAGGGG AGAGGTGGGC 
GTGCCCGGCC CAAGAGGAGA AGATGGTCCT GAGGGGCCAA AGGGTCGCGT CGGACCCCCT 
GGTGAAATTG GTCCCCTCGG CCTGTTGGGA GAGAAGGGTA AACTTGGTGT TCCTGGACTT 
CCTGGATATC CTGGACGTCA AGGAATAAAG GGGTCCCTTG GTTTCCCAGG ATTCCCTGGT 
ACAAATGGCG AGAAGGGCAC AAGGGGTTTG ATTGGAAAAC CTGGACCAAG AGGACAAAGA 
GGGCCGACAG GTCCTAGAGG GCAGAGAGGA CCACGTGGGG CAACTGGGAA ATCAGGTGCT 
AAGGGTGGAT CAGGAAGTGA CGGGCCTCCT GGACCACCAG GAGAGAGAGG GCTAACTGGA 
CCTCAGGGTG CTAATGGATT CCCTGGGCCA AAGGGACCTC CCGGACCTCC AGGGAAAGAT 
GGACTTCCTG GACACCCTGG ACAGAGAGGC GAAGTCGGTT TCCAAGGCAA AGTTGGACCA 
CCTGGCCCAC CTGGAGTTGT TGGCCCACAC GGTCCCTCTG GTGAGACTGG TCAGATGGGT 
GAGCGTGGCC ACCCTGGACC TCCAGGACCA CCTGGTGAGC AAGGCCTTTC TGGCTCCTCT 
GGTAAAGAAG GAACCAAAGG AGATCCCGGT CCTCCTGGTG GCCCCGGTAA AGATGGACCC 
CCTGGACTTA GAGGATTCCC AGGAGAGAGA GGACTACCAG GAACTCCAGG TTCTGGAGGA 
CTGAAAGGAA ATGAAGGCCC TGCTGGACCA CCTGGTCCTG CGGGTTCATC TGGGGAGAGA 
GGTGGTGCTG GCACTGCAGG CCCTGTCGGC CCACCTGGAC GACCTGGACC TCAGGGACCC 
CCTGGAACTT CAGGTGAAAA GGGAGTACCA GGCGAGAAAG GTCCGGTTGG TCCAGCTGGT 
CGTGATGGCA TCCAAGGCCC AGTTGGTCTC CCAGGCCCTG CCGGACCTCC AGGTATTTCT 
GGAGAAGATG GAGACAAGGG TGAGGTTGGA GAGCCAGGAC AAAAGGGAGC TAAAGGGTCC 
AAAGGAGAAC ATGGTCCTCC TGGTCCACCT GGGCCGATGG GTCCTGTTGG ACAGCCTGGT 
GCAGCGGGTG CTGATGGTGA GACGGGTCCA AGAGGTCAAC AGGGACAGTT TGGTGCCAAG 
GGAGATGAAG GCACAAGAGG TTTCCCTGGT CCCCCTGGCC CCATAGGATT GCAGGGTTTG 
CCAGGCCCAG GTGGAGAAAA GGGAGAGACA GGAGATGTTG GACCCTTGGG TCCTCCTGGT 
CCTCCAGGGC CGCGTGGTCC TGCTGGTCCT AATGGTGCTG ATGGTCCCCA AGGTCCTCCA 
GGAGGTTTGG GTAATCCAGG TCCCATAGGA GAGAAGGGTG AGCCTGGTGA ATCTGGACCA 
CCTGGTGTTG GTGGAGAGCC AGGAAAATTG GGCCCAAGAG GAGAGCGTGG TGAGAAGGGA 
GAGTCCGGGC AGCCTGGCAC CCCTGGTCCT CCTGGAGGAA AGGGACCCAC TGGAGATGAC 
GGACCCAAAG GAAACCCTGG TCCTGTTGGA TTCCCTGGTG ATCCTGGACC CCCTGGTGAA 
GTTGGACCAC GAGGTCAAGA TGGTGCAAAG GGTGACAGAG GAGAGGATGG AGAACAAGGA 
GAAGCCGGCT CACCTGGACC TACTGGAGAG AACGGACCAC CTGGACCTCC AGGAAAGAGA 
GGTCCTGCAG GCACAAGGGG ACCGGAGGGC CGTCAAGGAG AGAAAGGAAG CAAGGGTGAT 
TCAGGTGCTC TTGGTCCTCC CGGAAAAACT GGCCCTGTGG GGCCACAGGG ACAACCCGGA 
AAACCAGGAA CTGAGGGTCT AAGAGGGTTG CCTGGAACAG TGGGTGAGCA AGGTTCTCCA 
GGTGCTGCAG GCCCTAAAGG ACCTCCTGGA CCTCTGGGTC CTCCAGGTCT GGCTGGTCTT 
CGCGGTGATC CCGGTGCTAA AGGTGAGAAG GGACATCCAG GTATGCTCGG ACTGATCGGG 
CCTGCTGGAG AACAAGGAGA GAAGGGAGAC AGAGGCATGC CAGGACCACA GGGATCAACA 
GGACCCAAGG GAGAAACGGG TATATCTGGA GGAACTGGTC CACTTGGTCC TGCAGGTCCT 
GCTGGTATGC CTGGCCCGAG GGGTGTCAAA GGAGCTAAAG GTGCCAGTGG TGGGGCTGGT 
CCTAAAGGAG AAAAGGGTGT ACAAGGACCA CCAGGACCAC CTGGTCCCCC TGGTGATGTC 
ATCCAGCCGC TGCCCATTCA GATCCCCAAG AAGTCCAAGC GCTCCATCGA CGCCAGTAAG 
ATTCTGTCTG AGGCTGACGC TGAGGCTGCC GACACCACTG GCTCTGAGTT CCTGACTGGT 
AATGAAGGAA TGGAGGAGAT CTTCGGCTCA CTTAATTCCC TGCGCCAGGA GATCGAGAGC 
ATGCGCTTCC CATTGGGCAC AAAGGAGAGC CCCGGCCGCA CCTGCCAGGA TCTCCATCTC 
AGCCAACCAG ATCTGAAAGA CGGCGAGTAT TGGATTGATC CAAATCAAGG CTGTGCTCGC 
GACTCTTTCA AGGTTTACTG TAACTTCACT GCCGGCGGAG AGACCTGCCT GTATCCCAGC 
AAAGCAGTGG AGAATGTAAA GATGAACTCT TGGATAAAGG AGAAGCCTGG GTCCTTGTTC 
AGTCAGTTTG CAAAGGGCAA CAAGTTTTCA TATGTAGACT CGGTTGGAGA GGCTGTGGGT 
GTTGTTCAGC TGGGCTTCTT GCGGCTGCTC AGTGTCCAGG CCAGACAGAA CCTCACCTAC 
CACTGCCAAC GCTCGGTGGC CTGGACTGAC CAAACCACCG ACGGCGGATA CAAGCGGGCG 
CTGCGTCTGC AGGGGGCCAA TGATGAGGAG ATCAGCTATG AGACTAGCCC ATATATCAAA 
GCCCTCATCG ACGGCTGCTC CTATCGTAAG GGTCTGGACA GGACGGTGCT GGAGGTGAAC 
ACACCTCAGG TGGAGCAGCT CCCTCTGCTG GACATCAGGA TCTCAGACTT TGGGGAGGAC 
AATCAGAAAT TTGGTTTTGA AGTAGGACCT GTCTGTTTCC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159445.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159502.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa23891
Clone ID Related Accession (Same CDS sequence)	XM_005159445.4 , XM_005159445.3
Accession Version	XM_005159445.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159445.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGCCTCAGT GCGAGCCATT AAGGGAGAGA AGGGAGAGCC TGCTGTCCTT 
GAACCTGGCA TGCTGATTGA AGGACCACCA GGACCAGAAG GACCAGCAGG TCTCCCTGGC 
CCCTCTGGCC CCCAAGGACA ACCTGGTTCT GTTGGTGATC CTGGTGAGAG GGGTCCTACT 
GGTAAGGCTG GTCTACCTGG AGCAGATGGT GTTCCAGGAC CTCCAGGAAC CTCTGTCATG 
CTTCCTTTCC GCTTTGGACA GAGCGCTGGA GAGAAAGGAC CTGTTGCCTC TGCACAGGAA 
GCCCAAGCTC AAGCCATTCT GTCTCAAGCT CGGCTTGCCC TGAAAGGACC ATCCGGGCCA 
ATGGGTTTCA CTGGCCGTCC TGGACCTTTG GGAACACCTG GAAGTCCTGG CTTTAAGGGA 
GAAAGGGGGG ACCCTGGTGC TCAGGGTCCT AGAGGTCCTC AGGGTGTGTC TGGTCCTCCT 
GGCAAAGCTG GTCGGAGGGG TCGCGCTGGT GCTGATGGAG CCAGAGGGAT GCCAGGAGAG 
TCTGGAATTA AGGGTGACCG TGGTTTTGAT GGCCTTCCTG GTTTGCCTGG TGACAAGGGT 
TATCGGGGTC AACCTGGAGG AATGGGACCC CCAGGACCAC ATGGAGAGGA CGGAGAGAGG 
GGAGATGATG GAGATGTTGG ACCCAGAGGT CTTCCTGGCG AACCTGGACC CCGTGGTTTG 
TTGGGGCCTA AAGGGCCTTC TGGACTTCCT GGACCTCCTG GTGTTCGTGG AAATGATGGT 
CCACATGGTC CAAAAGGAAA CTTAGGACCA CAAGGAGAGC CCGGTCCTCC AGGCCAGCAG 
GGGGCTCCAG GAACACAGGG AATGCCAGGT CCACAGGGTG CCACTGGACC ACCAGGAGAG 
AAAGGACCCA CAGGAAAGCC AGGTCTACCA GGAATGCCAG GAGCTGATGG TCCTCCCGGT 
CATCCCGGGA AGGAAGGTCC TGGTGGAACC AAAGGAAATC AAGGTCCTAA TGGTCCTCAG 
GGCTCTATTG GCTATCCTGG CCCTCGGGGA TTGAAGGGAG CTCAAGGTAT CCGTGGACTG 
AAAGGTCATA AAGGAGAGAA GGGAGAGGAT GGATTCCCAG GAATTAAAGG AGACTTTGGT 
GTGAAGGGAG AGAGGGGAGA GGTGGGCGTG CCCGGCCCAA GAGGAGAAGA TGGTCCTGAG 
GGGCCAAAGG GTCGCGTCGG ACCCCCTGGT GAAATTGGTC CCCTCGGCCT GTTGGGAGAG 
AAGGGTAAAC TTGGTGTTCC TGGACTTCCT GGATATCCTG GACGTCAAGG AATAAAGGGG 
TCCCTTGGTT TCCCAGGATT CCCTGGTACA AATGGCGAGA AGGGCACAAG GGGTTTGATT 
GGAAAACCTG GACCAAGAGG ACAAAGAGGG CCGACAGGTC CTAGAGGGCA GAGAGGACCA 
CGTGGGGCAA CTGGGAAATC AGGTGCTAAG GGTGGATCAG GAAGTGACGG GCCTCCTGGA 
CCACCAGGAG AGAGAGGGCT AACTGGACCT CAGGGTGCTA ATGGATTCCC TGGGCCAAAG 
GGACCTCCCG GACCTCCAGG GAAAGATGGA CTTCCTGGAC ACCCTGGACA GAGAGGCGAA 
GTCGGTTTCC AAGGCAAAGT TGGACCACCT GGCCCACCTG GAGTTGTTGG CCCACACGGT 
CCCTCTGGTG AGACTGGTCA GATGGGTGAG CGTGGCCACC CTGGACCTCC AGGACCACCT 
GGTGAGCAAG GCCTTTCTGG CTCCTCTGGT AAAGAAGGAA CCAAAGGAGA TCCCGGTCCT 
CCTGGTGGCC CCGGTAAAGA TGGACCCCCT GGACTTAGAG GATTCCCAGG AGAGAGAGGA 
CTACCAGGAA CTCCAGGTTC TGGAGGACTG AAAGGAAATG AAGGCCCTGC TGGACCACCT 
GGTCCTGCGG GTTCATCTGG GGAGAGAGGT GGTGCTGGCA CTGCAGGCCC TGTCGGCCCA 
CCTGGACGAC CTGGACCTCA GGGACCCCCT GGAACTTCAG GTGAAAAGGG AGTACCAGGC 
GAGAAAGGTC CGGTTGGTCC AGCTGGTCGT GATGGCATCC AAGGCCCAGT TGGTCTCCCA 
GGCCCTGCCG GACCTCCAGG TATTTCTGGA GAAGATGGAG ACAAGGGTGA GGTTGGAGAG 
CCAGGACAAA AGGGAGCTAA AGGGTCCAAA GGAGAACATG GTCCTCCTGG TCCACCTGGG 
CCGATGGGTC CTGTTGGACA GCCTGGTGCA GCGGGTGCTG ATGGTGAGAC GGGTCCAAGA 
GGTCAACAGG GACAGTTTGG TGCCAAGGGA GATGAAGGCA CAAGAGGTTT CCCTGGTCCC 
CCTGGCCCCA TAGGATTGCA GGGTTTGCCA GGCCCAGGTG GAGAAAAGGG AGAGACAGGA 
GATGTTGGAC CCTTGGGTCC TCCTGGTCCT CCAGGGCCGC GTGGTCCTGC TGGTCCTAAT 
GGTGCTGATG GTCCCCAAGG TCCTCCAGGA GGTTTGGGTA ATCCAGGTCC CATAGGAGAG 
AAGGGTGAGC CTGGTGAATC TGGACCACCT GGTGTTGGTG GAGAGCCAGG AAAATTGGGC 
CCAAGAGGAG AGCGTGGTGA GAAGGGAGAG TCCGGGCAGC CTGGCACCCC TGGTCCTCCT 
GGAGGAAAGG GACCCACTGG AGATGACGGA CCCAAAGGAA ACCCTGGTCC TGTTGGATTC 
CCTGGTGATC CTGGACCCCC TGGTGAAGTT GGACCACGAG GTCAAGATGG TGCAAAGGGT 
GACAGAGGAG AGGATGGAGA ACAAGGAGAA GCCGGCTCAC CTGGACCTAC TGGAGAGAAC 
GGACCACCTG GACCTCCAGG AAAGAGAGGT CCTGCAGGCA CAAGGGGACC GGAGGGCCGT 
CAAGGAGAGA AAGGAAGCAA GGGTGATTCA GGTGCTCTTG GTCCTCCCGG AAAAACTGGC 
CCTGTGGGGC CACAGGGACA ACCCGGAAAA CCAGGAACTG AGGGTCTAAG AGGGTTGCCT 
GGAACAGTGG GTGAGCAAGG TTCTCCAGGT GCTGCAGGCC CTAAAGGACC TCCTGGACCT 
CTGGGTCCTC CAGGTCTGGC TGGTCTTCGC GGTGATCCCG GTGCTAAAGG TGAGAAGGGA 
CATCCAGGTA TGCTCGGACT GATCGGGCCT GCTGGAGAAC AAGGAGAGAA GGGAGACAGA 
GGCATGCCAG GACCACAGGG ATCAACAGGA CCCAAGGGAG AAACGGGTAT ATCTGGAGGA 
ACTGGTCCAC TTGGTCCTGC AGGTCCTGCT GGTATGCCTG GCCCGAGGGG TGTCAAAGGA 
GCTAAAGGTG CCAGTGGTGG GGCTGGTCCT AAAGGAGAAA AGGGTGTACA AGGACCACCA 
GGACCACCTG GTCCCCCTGG TGATGTCATC CAGCCGCTGC CCATTCAGAT CCCCAAGAAG 
TCCAAGCGCT CCATCGACGC CAGTAAGATT CTGTCTGAGG CTGACGCTGA GGCTGCCGAC 
ACCACTGGCT CTGAGTTCCT GACTGGTAAT GAAGGAATGG AGGAGATCTT CGGCTCACTT 
AATTCCCTGC GCCAGGAGAT CGAGAGCATG CGCTTCCCAT TGGGCACAAA GGAGAGCCCC 
GGCCGCACCT GCCAGGATCT CCATCTCAGC CAACCAGATC TGAAAGACGG CGAGTATTGG 
ATTGATCCAA ATCAAGGCTG TGCTCGCGAC TCTTTCAAGG TTTACTGTAA CTTCACTGCC 
GGCGGAGAGA CCTGCCTGTA TCCCAGCAAA GCAGTGGAGA ATGTAAAGAT GAACTCTTGG 
ATAAAGGAGA AGCCTGGGTC CTTGTTCAGT CAGTTTGCAA AGGGCAACAA GTTTTCATAT 
GTAGACTCGG TTGGAGAGGC TGTGGGTGTT GTTCAGCTGG GCTTCTTGCG GCTGCTCAGT 
GTCCAGGCCA GACAGAACCT CACCTACCAC TGCCAACGCT CGGTGGCCTG GACTGACCAA 
ACCACCGACG GCGGATACAA GCGGGCGCTG CGTCTGCAGG GGGCCAATGA TGAGGAGATC 
AGCTATGAGA CTAGCCCATA TATCAAAGCC CTCATCGACG GCTGCTCCTA TCGTAAGGGT 
CTGGACAGGA CGGTGCTGGA GGTGAACACA CCTCAGGTGG AGCAGCTCCC TCTGCTGGAC 
ATCAGGATCT CAGACTTTGG GGAGGACAAT CAGAAATTTG GTTTTGAAGT AGGACCTGTC 
TGTTTCCTGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159502.1
CDS	395..6109
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYAGKANNGKVTKQKTAPAAKPAPAKPAKSKPTPAPKTAKSKPTAAQLLISKIKPTPASKLAAKPKATPAPKNDKKKTNDNGKVNNGQTKVNGNGNGKSNGNGKDTNGKAKAGSNGKPNNNGNGNGKKTNGDAKINGNGPKTVKTVTKVASAKKSETPATKTVTGKPKAQVKAQPTKSKTDKTKANASPAKPKDTKAKAASVTQKPAVFKPTPVPLPRPSKTILDVNNVKSMSRNFPPFDKAVVSATTIKKKPTNGYQQASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159445.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159445.4

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGCCTCAGT GCGAGCCATT AAGGGAGAGA AGGGAGAGCC TGCTGTCCTT 
GAACCTGGCA TGCTGATTGA AGGACCACCA GGACCAGAAG GACCAGCAGG TCTCCCTGGC 
CCCTCTGGCC CCCAAGGACA ACCTGGTTCT GTTGGTGATC CTGGTGAGAG GGGTCCTACT 
GGTAAGGCTG GTCTACCTGG AGCAGATGGT GTTCCAGGAC CTCCAGGAAC CTCTGTCATG 
CTTCCTTTCC GCTTTGGACA GAGCGCTGGA GAGAAAGGAC CTGTTGCCTC TGCACAGGAA 
GCCCAAGCTC AAGCCATTCT GTCTCAAGCT CGGCTTGCCC TGAAAGGACC ATCCGGGCCA 
ATGGGTTTCA CTGGCCGTCC TGGACCTTTG GGAACACCTG GAAGTCCTGG CTTTAAGGGA 
GAAAGGGGGG ACCCTGGTGC TCAGGGTCCT AGAGGTCCTC AGGGTGTGTC TGGTCCTCCT 
GGCAAAGCTG GTCGGAGGGG TCGCGCTGGT GCTGATGGAG CCAGAGGGAT GCCAGGAGAG 
TCTGGAATTA AGGGTGACCG TGGTTTTGAT GGCCTTCCTG GTTTGCCTGG TGACAAGGGT 
TATCGGGGTC AACCTGGAGG AATGGGACCC CCAGGACCAC ATGGAGAGGA CGGAGAGAGG 
GGAGATGATG GAGATGTTGG ACCCAGAGGT CTTCCTGGCG AACCTGGACC CCGTGGTTTG 
TTGGGGCCTA AAGGGCCTTC TGGACTTCCT GGACCTCCTG GTGTTCGTGG AAATGATGGT 
CCACATGGTC CAAAAGGAAA CTTAGGACCA CAAGGAGAGC CCGGTCCTCC AGGCCAGCAG 
GGGGCTCCAG GAACACAGGG AATGCCAGGT CCACAGGGTG CCACTGGACC ACCAGGAGAG 
AAAGGACCCA CAGGAAAGCC AGGTCTACCA GGAATGCCAG GAGCTGATGG TCCTCCCGGT 
CATCCCGGGA AGGAAGGTCC TGGTGGAACC AAAGGAAATC AAGGTCCTAA TGGTCCTCAG 
GGCTCTATTG GCTATCCTGG CCCTCGGGGA TTGAAGGGAG CTCAAGGTAT CCGTGGACTG 
AAAGGTCATA AAGGAGAGAA GGGAGAGGAT GGATTCCCAG GAATTAAAGG AGACTTTGGT 
GTGAAGGGAG AGAGGGGAGA GGTGGGCGTG CCCGGCCCAA GAGGAGAAGA TGGTCCTGAG 
GGGCCAAAGG GTCGCGTCGG ACCCCCTGGT GAAATTGGTC CCCTCGGCCT GTTGGGAGAG 
AAGGGTAAAC TTGGTGTTCC TGGACTTCCT GGATATCCTG GACGTCAAGG AATAAAGGGG 
TCCCTTGGTT TCCCAGGATT CCCTGGTACA AATGGCGAGA AGGGCACAAG GGGTTTGATT 
GGAAAACCTG GACCAAGAGG ACAAAGAGGG CCGACAGGTC CTAGAGGGCA GAGAGGACCA 
CGTGGGGCAA CTGGGAAATC AGGTGCTAAG GGTGGATCAG GAAGTGACGG GCCTCCTGGA 
CCACCAGGAG AGAGAGGGCT AACTGGACCT CAGGGTGCTA ATGGATTCCC TGGGCCAAAG 
GGACCTCCCG GACCTCCAGG GAAAGATGGA CTTCCTGGAC ACCCTGGACA GAGAGGCGAA 
GTCGGTTTCC AAGGCAAAGT TGGACCACCT GGCCCACCTG GAGTTGTTGG CCCACACGGT 
CCCTCTGGTG AGACTGGTCA GATGGGTGAG CGTGGCCACC CTGGACCTCC AGGACCACCT 
GGTGAGCAAG GCCTTTCTGG CTCCTCTGGT AAAGAAGGAA CCAAAGGAGA TCCCGGTCCT 
CCTGGTGGCC CCGGTAAAGA TGGACCCCCT GGACTTAGAG GATTCCCAGG AGAGAGAGGA 
CTACCAGGAA CTCCAGGTTC TGGAGGACTG AAAGGAAATG AAGGCCCTGC TGGACCACCT 
GGTCCTGCGG GTTCATCTGG GGAGAGAGGT GGTGCTGGCA CTGCAGGCCC TGTCGGCCCA 
CCTGGACGAC CTGGACCTCA GGGACCCCCT GGAACTTCAG GTGAAAAGGG AGTACCAGGC 
GAGAAAGGTC CGGTTGGTCC AGCTGGTCGT GATGGCATCC AAGGCCCAGT TGGTCTCCCA 
GGCCCTGCCG GACCTCCAGG TATTTCTGGA GAAGATGGAG ACAAGGGTGA GGTTGGAGAG 
CCAGGACAAA AGGGAGCTAA AGGGTCCAAA GGAGAACATG GTCCTCCTGG TCCACCTGGG 
CCGATGGGTC CTGTTGGACA GCCTGGTGCA GCGGGTGCTG ATGGTGAGAC GGGTCCAAGA 
GGTCAACAGG GACAGTTTGG TGCCAAGGGA GATGAAGGCA CAAGAGGTTT CCCTGGTCCC 
CCTGGCCCCA TAGGATTGCA GGGTTTGCCA GGCCCAGGTG GAGAAAAGGG AGAGACAGGA 
GATGTTGGAC CCTTGGGTCC TCCTGGTCCT CCAGGGCCGC GTGGTCCTGC TGGTCCTAAT 
GGTGCTGATG GTCCCCAAGG TCCTCCAGGA GGTTTGGGTA ATCCAGGTCC CATAGGAGAG 
AAGGGTGAGC CTGGTGAATC TGGACCACCT GGTGTTGGTG GAGAGCCAGG AAAATTGGGC 
CCAAGAGGAG AGCGTGGTGA GAAGGGAGAG TCCGGGCAGC CTGGCACCCC TGGTCCTCCT 
GGAGGAAAGG GACCCACTGG AGATGACGGA CCCAAAGGAA ACCCTGGTCC TGTTGGATTC 
CCTGGTGATC CTGGACCCCC TGGTGAAGTT GGACCACGAG GTCAAGATGG TGCAAAGGGT 
GACAGAGGAG AGGATGGAGA ACAAGGAGAA GCCGGCTCAC CTGGACCTAC TGGAGAGAAC 
GGACCACCTG GACCTCCAGG AAAGAGAGGT CCTGCAGGCA CAAGGGGACC GGAGGGCCGT 
CAAGGAGAGA AAGGAAGCAA GGGTGATTCA GGTGCTCTTG GTCCTCCCGG AAAAACTGGC 
CCTGTGGGGC CACAGGGACA ACCCGGAAAA CCAGGAACTG AGGGTCTAAG AGGGTTGCCT 
GGAACAGTGG GTGAGCAAGG TTCTCCAGGT GCTGCAGGCC CTAAAGGACC TCCTGGACCT 
CTGGGTCCTC CAGGTCTGGC TGGTCTTCGC GGTGATCCCG GTGCTAAAGG TGAGAAGGGA 
CATCCAGGTA TGCTCGGACT GATCGGGCCT GCTGGAGAAC AAGGAGAGAA GGGAGACAGA 
GGCATGCCAG GACCACAGGG ATCAACAGGA CCCAAGGGAG AAACGGGTAT ATCTGGAGGA 
ACTGGTCCAC TTGGTCCTGC AGGTCCTGCT GGTATGCCTG GCCCGAGGGG TGTCAAAGGA 
GCTAAAGGTG CCAGTGGTGG GGCTGGTCCT AAAGGAGAAA AGGGTGTACA AGGACCACCA 
GGACCACCTG GTCCCCCTGG TGATGTCATC CAGCCGCTGC CCATTCAGAT CCCCAAGAAG 
TCCAAGCGCT CCATCGACGC CAGTAAGATT CTGTCTGAGG CTGACGCTGA GGCTGCCGAC 
ACCACTGGCT CTGAGTTCCT GACTGGTAAT GAAGGAATGG AGGAGATCTT CGGCTCACTT 
AATTCCCTGC GCCAGGAGAT CGAGAGCATG CGCTTCCCAT TGGGCACAAA GGAGAGCCCC 
GGCCGCACCT GCCAGGATCT CCATCTCAGC CAACCAGATC TGAAAGACGG CGAGTATTGG 
ATTGATCCAA ATCAAGGCTG TGCTCGCGAC TCTTTCAAGG TTTACTGTAA CTTCACTGCC 
GGCGGAGAGA CCTGCCTGTA TCCCAGCAAA GCAGTGGAGA ATGTAAAGAT GAACTCTTGG 
ATAAAGGAGA AGCCTGGGTC CTTGTTCAGT CAGTTTGCAA AGGGCAACAA GTTTTCATAT 
GTAGACTCGG TTGGAGAGGC TGTGGGTGTT GTTCAGCTGG GCTTCTTGCG GCTGCTCAGT 
GTCCAGGCCA GACAGAACCT CACCTACCAC TGCCAACGCT CGGTGGCCTG GACTGACCAA 
ACCACCGACG GCGGATACAA GCGGGCGCTG CGTCTGCAGG GGGCCAATGA TGAGGAGATC 
AGCTATGAGA CTAGCCCATA TATCAAAGCC CTCATCGACG GCTGCTCCTA TCGTAAGGGT 
CTGGACAGGA CGGTGCTGGA GGTGAACACA CCTCAGGTGG AGCAGCTCCC TCTGCTGGAC 
ATCAGGATCT CAGACTTTGG GGAGGACAAT CAGAAATTTG GTTTTGAAGT AGGACCTGTC 
TGTTTCCTGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159445.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159502.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa23891
Clone ID Related Accession (Same CDS sequence)	XM_005159445.4 , XM_005159445.3
Accession Version	XM_005159445.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(XI) chain isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159445.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGCCTCAGT GCGAGCCATT AAGGGAGAGA AGGGAGAGCC TGCTGTCCTT 
GAACCTGGCA TGCTGATTGA AGGACCACCA GGACCAGAAG GACCAGCAGG TCTCCCTGGC 
CCCTCTGGCC CCCAAGGACA ACCTGGTTCT GTTGGTGATC CTGGTGAGAG GGGTCCTACT 
GGTAAGGCTG GTCTACCTGG AGCAGATGGT GTTCCAGGAC CTCCAGGAAC CTCTGTCATG 
CTTCCTTTCC GCTTTGGACA GAGCGCTGGA GAGAAAGGAC CTGTTGCCTC TGCACAGGAA 
GCCCAAGCTC AAGCCATTCT GTCTCAAGCT CGGCTTGCCC TGAAAGGACC ATCCGGGCCA 
ATGGGTTTCA CTGGCCGTCC TGGACCTTTG GGAACACCTG GAAGTCCTGG CTTTAAGGGA 
GAAAGGGGGG ACCCTGGTGC TCAGGGTCCT AGAGGTCCTC AGGGTGTGTC TGGTCCTCCT 
GGCAAAGCTG GTCGGAGGGG TCGCGCTGGT GCTGATGGAG CCAGAGGGAT GCCAGGAGAG 
TCTGGAATTA AGGGTGACCG TGGTTTTGAT GGCCTTCCTG GTTTGCCTGG TGACAAGGGT 
TATCGGGGTC AACCTGGAGG AATGGGACCC CCAGGACCAC ATGGAGAGGA CGGAGAGAGG 
GGAGATGATG GAGATGTTGG ACCCAGAGGT CTTCCTGGCG AACCTGGACC CCGTGGTTTG 
TTGGGGCCTA AAGGGCCTTC TGGACTTCCT GGACCTCCTG GTGTTCGTGG AAATGATGGT 
CCACATGGTC CAAAAGGAAA CTTAGGACCA CAAGGAGAGC CCGGTCCTCC AGGCCAGCAG 
GGGGCTCCAG GAACACAGGG AATGCCAGGT CCACAGGGTG CCACTGGACC ACCAGGAGAG 
AAAGGACCCA CAGGAAAGCC AGGTCTACCA GGAATGCCAG GAGCTGATGG TCCTCCCGGT 
CATCCCGGGA AGGAAGGTCC TGGTGGAACC AAAGGAAATC AAGGTCCTAA TGGTCCTCAG 
GGCTCTATTG GCTATCCTGG CCCTCGGGGA TTGAAGGGAG CTCAAGGTAT CCGTGGACTG 
AAAGGTCATA AAGGAGAGAA GGGAGAGGAT GGATTCCCAG GAATTAAAGG AGACTTTGGT 
GTGAAGGGAG AGAGGGGAGA GGTGGGCGTG CCCGGCCCAA GAGGAGAAGA TGGTCCTGAG 
GGGCCAAAGG GTCGCGTCGG ACCCCCTGGT GAAATTGGTC CCCTCGGCCT GTTGGGAGAG 
AAGGGTAAAC TTGGTGTTCC TGGACTTCCT GGATATCCTG GACGTCAAGG AATAAAGGGG 
TCCCTTGGTT TCCCAGGATT CCCTGGTACA AATGGCGAGA AGGGCACAAG GGGTTTGATT 
GGAAAACCTG GACCAAGAGG ACAAAGAGGG CCGACAGGTC CTAGAGGGCA GAGAGGACCA 
CGTGGGGCAA CTGGGAAATC AGGTGCTAAG GGTGGATCAG GAAGTGACGG GCCTCCTGGA 
CCACCAGGAG AGAGAGGGCT AACTGGACCT CAGGGTGCTA ATGGATTCCC TGGGCCAAAG 
GGACCTCCCG GACCTCCAGG GAAAGATGGA CTTCCTGGAC ACCCTGGACA GAGAGGCGAA 
GTCGGTTTCC AAGGCAAAGT TGGACCACCT GGCCCACCTG GAGTTGTTGG CCCACACGGT 
CCCTCTGGTG AGACTGGTCA GATGGGTGAG CGTGGCCACC CTGGACCTCC AGGACCACCT 
GGTGAGCAAG GCCTTTCTGG CTCCTCTGGT AAAGAAGGAA CCAAAGGAGA TCCCGGTCCT 
CCTGGTGGCC CCGGTAAAGA TGGACCCCCT GGACTTAGAG GATTCCCAGG AGAGAGAGGA 
CTACCAGGAA CTCCAGGTTC TGGAGGACTG AAAGGAAATG AAGGCCCTGC TGGACCACCT 
GGTCCTGCGG GTTCATCTGG GGAGAGAGGT GGTGCTGGCA CTGCAGGCCC TGTCGGCCCA 
CCTGGACGAC CTGGACCTCA GGGACCCCCT GGAACTTCAG GTGAAAAGGG AGTACCAGGC 
GAGAAAGGTC CGGTTGGTCC AGCTGGTCGT GATGGCATCC AAGGCCCAGT TGGTCTCCCA 
GGCCCTGCCG GACCTCCAGG TATTTCTGGA GAAGATGGAG ACAAGGGTGA GGTTGGAGAG 
CCAGGACAAA AGGGAGCTAA AGGGTCCAAA GGAGAACATG GTCCTCCTGG TCCACCTGGG 
CCGATGGGTC CTGTTGGACA GCCTGGTGCA GCGGGTGCTG ATGGTGAGAC GGGTCCAAGA 
GGTCAACAGG GACAGTTTGG TGCCAAGGGA GATGAAGGCA CAAGAGGTTT CCCTGGTCCC 
CCTGGCCCCA TAGGATTGCA GGGTTTGCCA GGCCCAGGTG GAGAAAAGGG AGAGACAGGA 
GATGTTGGAC CCTTGGGTCC TCCTGGTCCT CCAGGGCCGC GTGGTCCTGC TGGTCCTAAT 
GGTGCTGATG GTCCCCAAGG TCCTCCAGGA GGTTTGGGTA ATCCAGGTCC CATAGGAGAG 
AAGGGTGAGC CTGGTGAATC TGGACCACCT GGTGTTGGTG GAGAGCCAGG AAAATTGGGC 
CCAAGAGGAG AGCGTGGTGA GAAGGGAGAG TCCGGGCAGC CTGGCACCCC TGGTCCTCCT 
GGAGGAAAGG GACCCACTGG AGATGACGGA CCCAAAGGAA ACCCTGGTCC TGTTGGATTC 
CCTGGTGATC CTGGACCCCC TGGTGAAGTT GGACCACGAG GTCAAGATGG TGCAAAGGGT 
GACAGAGGAG AGGATGGAGA ACAAGGAGAA GCCGGCTCAC CTGGACCTAC TGGAGAGAAC 
GGACCACCTG GACCTCCAGG AAAGAGAGGT CCTGCAGGCA CAAGGGGACC GGAGGGCCGT 
CAAGGAGAGA AAGGAAGCAA GGGTGATTCA GGTGCTCTTG GTCCTCCCGG AAAAACTGGC 
CCTGTGGGGC CACAGGGACA ACCCGGAAAA CCAGGAACTG AGGGTCTAAG AGGGTTGCCT 
GGAACAGTGG GTGAGCAAGG TTCTCCAGGT GCTGCAGGCC CTAAAGGACC TCCTGGACCT 
CTGGGTCCTC CAGGTCTGGC TGGTCTTCGC GGTGATCCCG GTGCTAAAGG TGAGAAGGGA 
CATCCAGGTA TGCTCGGACT GATCGGGCCT GCTGGAGAAC AAGGAGAGAA GGGAGACAGA 
GGCATGCCAG GACCACAGGG ATCAACAGGA CCCAAGGGAG AAACGGGTAT ATCTGGAGGA 
ACTGGTCCAC TTGGTCCTGC AGGTCCTGCT GGTATGCCTG GCCCGAGGGG TGTCAAAGGA 
GCTAAAGGTG CCAGTGGTGG GGCTGGTCCT AAAGGAGAAA AGGGTGTACA AGGACCACCA 
GGACCACCTG GTCCCCCTGG TGATGTCATC CAGCCGCTGC CCATTCAGAT CCCCAAGAAG 
TCCAAGCGCT CCATCGACGC CAGTAAGATT CTGTCTGAGG CTGACGCTGA GGCTGCCGAC 
ACCACTGGCT CTGAGTTCCT GACTGGTAAT GAAGGAATGG AGGAGATCTT CGGCTCACTT 
AATTCCCTGC GCCAGGAGAT CGAGAGCATG CGCTTCCCAT TGGGCACAAA GGAGAGCCCC 
GGCCGCACCT GCCAGGATCT CCATCTCAGC CAACCAGATC TGAAAGACGG CGAGTATTGG 
ATTGATCCAA ATCAAGGCTG TGCTCGCGAC TCTTTCAAGG TTTACTGTAA CTTCACTGCC 
GGCGGAGAGA CCTGCCTGTA TCCCAGCAAA GCAGTGGAGA ATGTAAAGAT GAACTCTTGG 
ATAAAGGAGA AGCCTGGGTC CTTGTTCAGT CAGTTTGCAA AGGGCAACAA GTTTTCATAT 
GTAGACTCGG TTGGAGAGGC TGTGGGTGTT GTTCAGCTGG GCTTCTTGCG GCTGCTCAGT 
GTCCAGGCCA GACAGAACCT CACCTACCAC TGCCAACGCT CGGTGGCCTG GACTGACCAA 
ACCACCGACG GCGGATACAA GCGGGCGCTG CGTCTGCAGG GGGCCAATGA TGAGGAGATC 
AGCTATGAGA CTAGCCCATA TATCAAAGCC CTCATCGACG GCTGCTCCTA TCGTAAGGGT 
CTGGACAGGA CGGTGCTGGA GGTGAACACA CCTCAGGTGG AGCAGCTCCC TCTGCTGGAC 
ATCAGGATCT CAGACTTTGG GGAGGACAAT CAGAAATTTG GTTTTGAAGT AGGACCTGTC 
TGTTTCCTGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159502.1
CDS	384..6098
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYAGKANNGKVTKQKTAPAAKPAPAKPAKSKPTPAPKTAKSKPTAAQLLISKIKPTPASKLAAKPKATPAPKNDKKKTNDNGKVNNGQTKVNGNGNGKSNGNGKDTNGKAKAGSNGKPNNNGNGNGKKTNGDAKINGNGPKTVKTVTKVASAKKSETPATKTVTGKPKAQVKAQPTKSKTDKTKANASPAKPKDTKAKAASVTQKPAVFKPTPVPLPRPSKTILDVNNVKSMSRNFPPFDKAVVSATTIKKKPTNGYQQASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159445.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159445.3

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGCCTCAGT GCGAGCCATT AAGGGAGAGA AGGGAGAGCC TGCTGTCCTT 
GAACCTGGCA TGCTGATTGA AGGACCACCA GGACCAGAAG GACCAGCAGG TCTCCCTGGC 
CCCTCTGGCC CCCAAGGACA ACCTGGTTCT GTTGGTGATC CTGGTGAGAG GGGTCCTACT 
GGTAAGGCTG GTCTACCTGG AGCAGATGGT GTTCCAGGAC CTCCAGGAAC CTCTGTCATG 
CTTCCTTTCC GCTTTGGACA GAGCGCTGGA GAGAAAGGAC CTGTTGCCTC TGCACAGGAA 
GCCCAAGCTC AAGCCATTCT GTCTCAAGCT CGGCTTGCCC TGAAAGGACC ATCCGGGCCA 
ATGGGTTTCA CTGGCCGTCC TGGACCTTTG GGAACACCTG GAAGTCCTGG CTTTAAGGGA 
GAAAGGGGGG ACCCTGGTGC TCAGGGTCCT AGAGGTCCTC AGGGTGTGTC TGGTCCTCCT 
GGCAAAGCTG GTCGGAGGGG TCGCGCTGGT GCTGATGGAG CCAGAGGGAT GCCAGGAGAG 
TCTGGAATTA AGGGTGACCG TGGTTTTGAT GGCCTTCCTG GTTTGCCTGG TGACAAGGGT 
TATCGGGGTC AACCTGGAGG AATGGGACCC CCAGGACCAC ATGGAGAGGA CGGAGAGAGG 
GGAGATGATG GAGATGTTGG ACCCAGAGGT CTTCCTGGCG AACCTGGACC CCGTGGTTTG 
TTGGGGCCTA AAGGGCCTTC TGGACTTCCT GGACCTCCTG GTGTTCGTGG AAATGATGGT 
CCACATGGTC CAAAAGGAAA CTTAGGACCA CAAGGAGAGC CCGGTCCTCC AGGCCAGCAG 
GGGGCTCCAG GAACACAGGG AATGCCAGGT CCACAGGGTG CCACTGGACC ACCAGGAGAG 
AAAGGACCCA CAGGAAAGCC AGGTCTACCA GGAATGCCAG GAGCTGATGG TCCTCCCGGT 
CATCCCGGGA AGGAAGGTCC TGGTGGAACC AAAGGAAATC AAGGTCCTAA TGGTCCTCAG 
GGCTCTATTG GCTATCCTGG CCCTCGGGGA TTGAAGGGAG CTCAAGGTAT CCGTGGACTG 
AAAGGTCATA AAGGAGAGAA GGGAGAGGAT GGATTCCCAG GAATTAAAGG AGACTTTGGT 
GTGAAGGGAG AGAGGGGAGA GGTGGGCGTG CCCGGCCCAA GAGGAGAAGA TGGTCCTGAG 
GGGCCAAAGG GTCGCGTCGG ACCCCCTGGT GAAATTGGTC CCCTCGGCCT GTTGGGAGAG 
AAGGGTAAAC TTGGTGTTCC TGGACTTCCT GGATATCCTG GACGTCAAGG AATAAAGGGG 
TCCCTTGGTT TCCCAGGATT CCCTGGTACA AATGGCGAGA AGGGCACAAG GGGTTTGATT 
GGAAAACCTG GACCAAGAGG ACAAAGAGGG CCGACAGGTC CTAGAGGGCA GAGAGGACCA 
CGTGGGGCAA CTGGGAAATC AGGTGCTAAG GGTGGATCAG GAAGTGACGG GCCTCCTGGA 
CCACCAGGAG AGAGAGGGCT AACTGGACCT CAGGGTGCTA ATGGATTCCC TGGGCCAAAG 
GGACCTCCCG GACCTCCAGG GAAAGATGGA CTTCCTGGAC ACCCTGGACA GAGAGGCGAA 
GTCGGTTTCC AAGGCAAAGT TGGACCACCT GGCCCACCTG GAGTTGTTGG CCCACACGGT 
CCCTCTGGTG AGACTGGTCA GATGGGTGAG CGTGGCCACC CTGGACCTCC AGGACCACCT 
GGTGAGCAAG GCCTTTCTGG CTCCTCTGGT AAAGAAGGAA CCAAAGGAGA TCCCGGTCCT 
CCTGGTGGCC CCGGTAAAGA TGGACCCCCT GGACTTAGAG GATTCCCAGG AGAGAGAGGA 
CTACCAGGAA CTCCAGGTTC TGGAGGACTG AAAGGAAATG AAGGCCCTGC TGGACCACCT 
GGTCCTGCGG GTTCATCTGG GGAGAGAGGT GGTGCTGGCA CTGCAGGCCC TGTCGGCCCA 
CCTGGACGAC CTGGACCTCA GGGACCCCCT GGAACTTCAG GTGAAAAGGG AGTACCAGGC 
GAGAAAGGTC CGGTTGGTCC AGCTGGTCGT GATGGCATCC AAGGCCCAGT TGGTCTCCCA 
GGCCCTGCCG GACCTCCAGG TATTTCTGGA GAAGATGGAG ACAAGGGTGA GGTTGGAGAG 
CCAGGACAAA AGGGAGCTAA AGGGTCCAAA GGAGAACATG GTCCTCCTGG TCCACCTGGG 
CCGATGGGTC CTGTTGGACA GCCTGGTGCA GCGGGTGCTG ATGGTGAGAC GGGTCCAAGA 
GGTCAACAGG GACAGTTTGG TGCCAAGGGA GATGAAGGCA CAAGAGGTTT CCCTGGTCCC 
CCTGGCCCCA TAGGATTGCA GGGTTTGCCA GGCCCAGGTG GAGAAAAGGG AGAGACAGGA 
GATGTTGGAC CCTTGGGTCC TCCTGGTCCT CCAGGGCCGC GTGGTCCTGC TGGTCCTAAT 
GGTGCTGATG GTCCCCAAGG TCCTCCAGGA GGTTTGGGTA ATCCAGGTCC CATAGGAGAG 
AAGGGTGAGC CTGGTGAATC TGGACCACCT GGTGTTGGTG GAGAGCCAGG AAAATTGGGC 
CCAAGAGGAG AGCGTGGTGA GAAGGGAGAG TCCGGGCAGC CTGGCACCCC TGGTCCTCCT 
GGAGGAAAGG GACCCACTGG AGATGACGGA CCCAAAGGAA ACCCTGGTCC TGTTGGATTC 
CCTGGTGATC CTGGACCCCC TGGTGAAGTT GGACCACGAG GTCAAGATGG TGCAAAGGGT 
GACAGAGGAG AGGATGGAGA ACAAGGAGAA GCCGGCTCAC CTGGACCTAC TGGAGAGAAC 
GGACCACCTG GACCTCCAGG AAAGAGAGGT CCTGCAGGCA CAAGGGGACC GGAGGGCCGT 
CAAGGAGAGA AAGGAAGCAA GGGTGATTCA GGTGCTCTTG GTCCTCCCGG AAAAACTGGC 
CCTGTGGGGC CACAGGGACA ACCCGGAAAA CCAGGAACTG AGGGTCTAAG AGGGTTGCCT 
GGAACAGTGG GTGAGCAAGG TTCTCCAGGT GCTGCAGGCC CTAAAGGACC TCCTGGACCT 
CTGGGTCCTC CAGGTCTGGC TGGTCTTCGC GGTGATCCCG GTGCTAAAGG TGAGAAGGGA 
CATCCAGGTA TGCTCGGACT GATCGGGCCT GCTGGAGAAC AAGGAGAGAA GGGAGACAGA 
GGCATGCCAG GACCACAGGG ATCAACAGGA CCCAAGGGAG AAACGGGTAT ATCTGGAGGA 
ACTGGTCCAC TTGGTCCTGC AGGTCCTGCT GGTATGCCTG GCCCGAGGGG TGTCAAAGGA 
GCTAAAGGTG CCAGTGGTGG GGCTGGTCCT AAAGGAGAAA AGGGTGTACA AGGACCACCA 
GGACCACCTG GTCCCCCTGG TGATGTCATC CAGCCGCTGC CCATTCAGAT CCCCAAGAAG 
TCCAAGCGCT CCATCGACGC CAGTAAGATT CTGTCTGAGG CTGACGCTGA GGCTGCCGAC 
ACCACTGGCT CTGAGTTCCT GACTGGTAAT GAAGGAATGG AGGAGATCTT CGGCTCACTT 
AATTCCCTGC GCCAGGAGAT CGAGAGCATG CGCTTCCCAT TGGGCACAAA GGAGAGCCCC 
GGCCGCACCT GCCAGGATCT CCATCTCAGC CAACCAGATC TGAAAGACGG CGAGTATTGG 
ATTGATCCAA ATCAAGGCTG TGCTCGCGAC TCTTTCAAGG TTTACTGTAA CTTCACTGCC 
GGCGGAGAGA CCTGCCTGTA TCCCAGCAAA GCAGTGGAGA ATGTAAAGAT GAACTCTTGG 
ATAAAGGAGA AGCCTGGGTC CTTGTTCAGT CAGTTTGCAA AGGGCAACAA GTTTTCATAT 
GTAGACTCGG TTGGAGAGGC TGTGGGTGTT GTTCAGCTGG GCTTCTTGCG GCTGCTCAGT 
GTCCAGGCCA GACAGAACCT CACCTACCAC TGCCAACGCT CGGTGGCCTG GACTGACCAA 
ACCACCGACG GCGGATACAA GCGGGCGCTG CGTCTGCAGG GGGCCAATGA TGAGGAGATC 
AGCTATGAGA CTAGCCCATA TATCAAAGCC CTCATCGACG GCTGCTCCTA TCGTAAGGGT 
CTGGACAGGA CGGTGCTGGA GGTGAACACA CCTCAGGTGG AGCAGCTCCC TCTGCTGGAC 
ATCAGGATCT CAGACTTTGG GGAGGACAAT CAGAAATTTG GTTTTGAAGT AGGACCTGTC 
TGTTTCCTGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_017352253.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207742.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X7, mRNA.

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CloneID	ODa23892
Clone ID Related Accession (Same CDS sequence)	NM_001328265.1 , XM_017352253.1 , XM_017352253.2
Accession Version	XM_017352253.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(XI) chain isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCACTTTGAT GAAGAGCAGG ATGATTATGA ATACCCCTAT 
TATTATGAAG GAACTACCAG TGTCCCTCCC TCCCCATCCA AACCTCCCTC CGTCCCAGCC 
GAGACACTCA CTGAACAGGA CGTAGATCCT GCTTACTCAG AGGCTGACCA CACCCCCACA 
GAGACCGATT ATTATTACAC AGTAGAAGAA GAGGCGGCTC CACAGCCAGA GAGTGATCTG 
ACTGGATATG ACGAAAGCAT TAACCAGGTC GACGAGACTG TCCCCGTTGA GGAAATGGAT 
GCTACCAAGA CAGGTGGTGA GGTGGCGTCT CAAACGCCAG AAGAGCCCTT CACTGAGGAG 
TACATTACAA GCGACTTTGG GATGAAGGAG TATGACTATT CCTACAAGGA CTACAATGAG 
CCCATCATTG AGAGCAAGAC GGAGGACACT CTAGGCCCTG CCCTGTCCGC TGTGACAGAG 
GAGGGGGGTG CCTCAGTGCG AGCCATTAAG GGAGAGAAGG GAGAGCCTGC TGTCCTTGAA 
CCTGGCATGC TGATTGAAGG ACCACCAGGA CCAGAAGGAC CAGCAGGTCT CCCTGGCCCC 
TCTGGCCCCC AAGGACAACC TGGTTCTGTT GGTGATCCTG GTGAGAGGGG TCCTACTGGT 
AAGGCTGGTC TACCTGGAGC AGATGGTGTT CCAGGACCTC CAGGAACCTC TGTCATGCTT 
CCTTTCCGCT TTGGACAGAG CGCTGGAGAG AAAGGACCTG TTGCCTCTGC ACAGGAAGCC 
CAAGCTCAAG CCATTCTGTC TCAAGCTCGG CTTGCCCTGA AAGGACCATC CGGGCCAATG 
GGTTTCACTG GCCGTCCTGG ACCTTTGGGA ACACCTGGAA GTCCTGGCTT TAAGGGAGAA 
AGGGGGGACC CTGGTGCTCA GGGTCCTAGA GGTCCTCAGG GTGTGTCTGG TCCTCCTGGC 
AAAGCTGGTC GGAGGGGTCG CGCTGGTGCT GATGGAGCCA GAGGGATGCC AGGAGAGTCT 
GGAATTAAGG GTGACCGTGG TTTTGATGGC CTTCCTGGTT TGCCTGGTGA CAAGGGTTAT 
CGGGGTCAAC CTGGAGGAAT GGGACCCCCA GGACCACATG GAGAGGACGG AGAGAGGGGA 
GATGATGGAG ATGTTGGACC CAGAGGTCTT CCTGGCGAAC CTGGACCCCG TGGTTTGTTG 
GGGCCTAAAG GGCCTTCTGG ACTTCCTGGA CCTCCTGGTG TTCGTGGAAA TGATGGTCCA 
CATGGTCCAA AAGGAAACTT AGGACCACAA GGAGAGCCCG GTCCTCCAGG CCAGCAGGGG 
GCTCCAGGAA CACAGGGAAT GCCAGGTCCA CAGGGTGCCA CTGGACCACC AGGAGAGAAA 
GGACCCACAG GAAAGCCAGG TCTACCAGGA ATGCCAGGAG CTGATGGTCC TCCCGGTCAT 
CCCGGGAAGG AAGGTCCTGG TGGAACCAAA GGAAATCAAG GTCCTAATGG TCCTCAGGGC 
TCTATTGGCT ATCCTGGCCC TCGGGGATTG AAGGGAGCTC AAGGTATCCG TGGACTGAAA 
GGTCATAAAG GAGAGAAGGG AGAGGATGGA TTCCCAGGAA TTAAAGGAGA CTTTGGTGTG 
AAGGGAGAGA GGGGAGAGGT GGGCGTGCCC GGCCCAAGAG GAGAAGATGG TCCTGAGGGG 
CCAAAGGGTC GCGTCGGACC CCCTGGTGAA ATTGGTCCCC TCGGCCTGTT GGGAGAGAAG 
GGTAAACTTG GTGTTCCTGG ACTTCCTGGA TATCCTGGAC GTCAAGGAAT AAAGGGGTCC 
CTTGGTTTCC CAGGATTCCC TGGTACAAAT GGCGAGAAGG GCACAAGGGG TTTGATTGGA 
AAACCTGGAC CAAGAGGACA AAGAGGGCCG ACAGGTCCTA GAGGGCAGAG AGGACCACGT 
GGGGCAACTG GGAAATCAGG TGCTAAGGGT GGATCAGGAA GTGACGGGCC TCCTGGACCA 
CCAGGAGAGA GAGGGCTAAC TGGACCTCAG GGTGCTAATG GATTCCCTGG GCCAAAGGGA 
CCTCCCGGAC CTCCAGGGAA AGATGGACTT CCTGGACACC CTGGACAGAG AGGCGAAGTC 
GGTTTCCAAG GCAAAGTTGG ACCACCTGGC CCACCTGGAG TTGTTGGCCC ACACGGTCCC 
TCTGGTGAGA CTGGTCAGAT GGGTGAGCGT GGCCACCCTG GACCTCCAGG ACCACCTGGT 
GAGCAAGGCC TTTCTGGCTC CTCTGGTAAA GAAGGAACCA AAGGAGATCC CGGTCCTCCT 
GGTGGCCCCG GTAAAGATGG ACCCCCTGGA CTTAGAGGAT TCCCAGGAGA GAGAGGACTA 
CCAGGAACTC CAGGTTCTGG AGGACTGAAA GGAAATGAAG GCCCTGCTGG ACCACCTGGT 
CCTGCGGGTT CATCTGGGGA GAGAGGTGGT GCTGGCACTG CAGGCCCTGT CGGCCCACCT 
GGACGACCTG GACCTCAGGG ACCCCCTGGA ACTTCAGGTG AAAAGGGAGT ACCAGGCGAG 
AAAGGTCCGG TTGGTCCAGC TGGTCGTGAT GGCATCCAAG GCCCAGTTGG TCTCCCAGGC 
CCTGCCGGAC CTCCAGGTAT TTCTGGAGAA GATGGAGACA AGGGTGAGGT TGGAGAGCCA 
GGACAAAAGG GAGCTAAAGG GTCCAAAGGA GAACATGGTC CTCCTGGTCC ACCTGGGCCG 
ATGGGTCCTG TTGGACAGCC TGGTGCAGCG GGTGCTGATG GTGAGACGGG TCCAAGAGGT 
CAACAGGGAC AGTTTGGTGC CAAGGGAGAT GAAGGCACAA GAGGTTTCCC TGGTCCCCCT 
GGCCCCATAG GATTGCAGGG TTTGCCAGGC CCAGGTGGAG AAAAGGGAGA GACAGGAGAT 
GTTGGACCCT TGGGTCCTCC TGGTCCTCCA GGGCCGCGTG GTCCTGCTGG TCCTAATGGT 
GCTGATGGTC CCCAAGGTCC TCCAGGAGGT TTGGGTAATC CAGGTCCCAT AGGAGAGAAG 
GGTGAGCCTG GTGAATCTGG ACCACCTGGT GTTGGTGGAG AGCCAGGAAA ATTGGGCCCA 
AGAGGAGAGC GTGGTGAGAA GGGAGAGTCC GGGCAGCCTG GCACCCCTGG TCCTCCTGGA 
GGAAAGGGAC CCACTGGAGA TGACGGACCC AAAGGAAACC CTGGTCCTGT TGGATTCCCT 
GGTGATCCTG GACCCCCTGG TGAAGTTGGA CCACGAGGTC AAGATGGTGC AAAGGGTGAC 
AGAGGAGAGG ATGGAGAACA AGGAGAAGCC GGCTCACCTG GACCTACTGG AGAGAACGGA 
CCACCTGGAC CTCCAGGAAA GAGAGGTCCT GCAGGCACAA GGGGACCGGA GGGCCGTCAA 
GGAGAGAAAG GAAGCAAGGG TGATTCAGGT GCTCTTGGTC CTCCCGGAAA AACTGGCCCT 
GTGGGGCCAC AGGGACAACC CGGAAAACCA GGAACTGAGG GTCTAAGAGG GTTGCCTGGA 
ACAGTGGGTG AGCAAGGTTC TCCAGGTGCT GCAGGCCCTA AAGGACCTCC TGGACCTCTG 
GGTCCTCCAG GTCTGGCTGG TCTTCGCGGT GATCCCGGTG CTAAAGGTGA GAAGGGACAT 
CCAGGTATGC TCGGACTGAT CGGGCCTGCT GGAGAACAAG GAGAGAAGGG AGACAGAGGC 
ATGCCAGGAC CACAGGGATC AACAGGACCC AAGGGAGAAA CGGGTATATC TGGAGGAACT 
GGTCCACTTG GTCCTGCAGG TCCTGCTGGT ATGCCTGGCC CGAGGGGTGT CAAAGGAGCT 
AAAGGTGCCA GTGGTGGGGC TGGTCCTAAA GGAGAAAAGG GTGTACAAGG ACCACCAGGA 
CCACCTGGTC CCCCTGGTGA TGTCATCCAG CCGCTGCCCA TTCAGATCCC CAAGAAGTCC 
AAGCGCTCCA TCGACGCCAG TAAGATTCTG TCTGAGGCTG ACGCTGAGGC TGCCGACACC 
ACTGGCTCTG AGTTCCTGAC TGGTAATGAA GGAATGGAGG AGATCTTCGG CTCACTTAAT 
TCCCTGCGCC AGGAGATCGA GAGCATGCGC TTCCCATTGG GCACAAAGGA GAGCCCCGGC 
CGCACCTGCC AGGATCTCCA TCTCAGCCAA CCAGATCTGA AAGACGGCGA GTATTGGATT 
GATCCAAATC AAGGCTGTGC TCGCGACTCT TTCAAGGTTT ACTGTAACTT CACTGCCGGC 
GGAGAGACCT GCCTGTATCC CAGCAAAGCA GTGGAGAATG TAAAGATGAA CTCTTGGATA 
AAGGAGAAGC CTGGGTCCTT GTTCAGTCAG TTTGCAAAGG GCAACAAGTT TTCATATGTA 
GACTCGGTTG GAGAGGCTGT GGGTGTTGTT CAGCTGGGCT TCTTGCGGCT GCTCAGTGTC 
CAGGCCAGAC AGAACCTCAC CTACCACTGC CAACGCTCGG TGGCCTGGAC TGACCAAACC 
ACCGACGGCG GATACAAGCG GGCGCTGCGT CTGCAGGGGG CCAATGATGA GGAGATCAGC 
TATGAGACTA GCCCATATAT CAAAGCCCTC ATCGACGGCT GCTCCTATCG TAAGGGTCTG 
GACAGGACGG TGCTGGAGGT GAACACACCT CAGGTGGAGC AGCTCCCTCT GCTGGACATC 
AGGATCTCAG ACTTTGGGGA GGACAATCAG AAATTTGGTT TTGAAGTAGG ACCTGTCTGT 
TTCCTGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207742.1
CDS	384..5795
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYHFDEEQDDYEYPYYYEGTTSVPPSPSKPPSVPAETLTEQDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_017352253.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352253.1

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCACTTTGAT GAAGAGCAGG ATGATTATGA ATACCCCTAT 
TATTATGAAG GAACTACCAG TGTCCCTCCC TCCCCATCCA AACCTCCCTC CGTCCCAGCC 
GAGACACTCA CTGAACAGGA CGTAGATCCT GCTTACTCAG AGGCTGACCA CACCCCCACA 
GAGACCGATT ATTATTACAC AGTAGAAGAA GAGGCGGCTC CACAGCCAGA GAGTGATCTG 
ACTGGATATG ACGAAAGCAT TAACCAGGTC GACGAGACTG TCCCCGTTGA GGAAATGGAT 
GCTACCAAGA CAGGTGGTGA GGTGGCGTCT CAAACGCCAG AAGAGCCCTT CACTGAGGAG 
TACATTACAA GCGACTTTGG GATGAAGGAG TATGACTATT CCTACAAGGA CTACAATGAG 
CCCATCATTG AGAGCAAGAC GGAGGACACT CTAGGCCCTG CCCTGTCCGC TGTGACAGAG 
GAGGGGGGTG CCTCAGTGCG AGCCATTAAG GGAGAGAAGG GAGAGCCTGC TGTCCTTGAA 
CCTGGCATGC TGATTGAAGG ACCACCAGGA CCAGAAGGAC CAGCAGGTCT CCCTGGCCCC 
TCTGGCCCCC AAGGACAACC TGGTTCTGTT GGTGATCCTG GTGAGAGGGG TCCTACTGGT 
AAGGCTGGTC TACCTGGAGC AGATGGTGTT CCAGGACCTC CAGGAACCTC TGTCATGCTT 
CCTTTCCGCT TTGGACAGAG CGCTGGAGAG AAAGGACCTG TTGCCTCTGC ACAGGAAGCC 
CAAGCTCAAG CCATTCTGTC TCAAGCTCGG CTTGCCCTGA AAGGACCATC CGGGCCAATG 
GGTTTCACTG GCCGTCCTGG ACCTTTGGGA ACACCTGGAA GTCCTGGCTT TAAGGGAGAA 
AGGGGGGACC CTGGTGCTCA GGGTCCTAGA GGTCCTCAGG GTGTGTCTGG TCCTCCTGGC 
AAAGCTGGTC GGAGGGGTCG CGCTGGTGCT GATGGAGCCA GAGGGATGCC AGGAGAGTCT 
GGAATTAAGG GTGACCGTGG TTTTGATGGC CTTCCTGGTT TGCCTGGTGA CAAGGGTTAT 
CGGGGTCAAC CTGGAGGAAT GGGACCCCCA GGACCACATG GAGAGGACGG AGAGAGGGGA 
GATGATGGAG ATGTTGGACC CAGAGGTCTT CCTGGCGAAC CTGGACCCCG TGGTTTGTTG 
GGGCCTAAAG GGCCTTCTGG ACTTCCTGGA CCTCCTGGTG TTCGTGGAAA TGATGGTCCA 
CATGGTCCAA AAGGAAACTT AGGACCACAA GGAGAGCCCG GTCCTCCAGG CCAGCAGGGG 
GCTCCAGGAA CACAGGGAAT GCCAGGTCCA CAGGGTGCCA CTGGACCACC AGGAGAGAAA 
GGACCCACAG GAAAGCCAGG TCTACCAGGA ATGCCAGGAG CTGATGGTCC TCCCGGTCAT 
CCCGGGAAGG AAGGTCCTGG TGGAACCAAA GGAAATCAAG GTCCTAATGG TCCTCAGGGC 
TCTATTGGCT ATCCTGGCCC TCGGGGATTG AAGGGAGCTC AAGGTATCCG TGGACTGAAA 
GGTCATAAAG GAGAGAAGGG AGAGGATGGA TTCCCAGGAA TTAAAGGAGA CTTTGGTGTG 
AAGGGAGAGA GGGGAGAGGT GGGCGTGCCC GGCCCAAGAG GAGAAGATGG TCCTGAGGGG 
CCAAAGGGTC GCGTCGGACC CCCTGGTGAA ATTGGTCCCC TCGGCCTGTT GGGAGAGAAG 
GGTAAACTTG GTGTTCCTGG ACTTCCTGGA TATCCTGGAC GTCAAGGAAT AAAGGGGTCC 
CTTGGTTTCC CAGGATTCCC TGGTACAAAT GGCGAGAAGG GCACAAGGGG TTTGATTGGA 
AAACCTGGAC CAAGAGGACA AAGAGGGCCG ACAGGTCCTA GAGGGCAGAG AGGACCACGT 
GGGGCAACTG GGAAATCAGG TGCTAAGGGT GGATCAGGAA GTGACGGGCC TCCTGGACCA 
CCAGGAGAGA GAGGGCTAAC TGGACCTCAG GGTGCTAATG GATTCCCTGG GCCAAAGGGA 
CCTCCCGGAC CTCCAGGGAA AGATGGACTT CCTGGACACC CTGGACAGAG AGGCGAAGTC 
GGTTTCCAAG GCAAAGTTGG ACCACCTGGC CCACCTGGAG TTGTTGGCCC ACACGGTCCC 
TCTGGTGAGA CTGGTCAGAT GGGTGAGCGT GGCCACCCTG GACCTCCAGG ACCACCTGGT 
GAGCAAGGCC TTTCTGGCTC CTCTGGTAAA GAAGGAACCA AAGGAGATCC CGGTCCTCCT 
GGTGGCCCCG GTAAAGATGG ACCCCCTGGA CTTAGAGGAT TCCCAGGAGA GAGAGGACTA 
CCAGGAACTC CAGGTTCTGG AGGACTGAAA GGAAATGAAG GCCCTGCTGG ACCACCTGGT 
CCTGCGGGTT CATCTGGGGA GAGAGGTGGT GCTGGCACTG CAGGCCCTGT CGGCCCACCT 
GGACGACCTG GACCTCAGGG ACCCCCTGGA ACTTCAGGTG AAAAGGGAGT ACCAGGCGAG 
AAAGGTCCGG TTGGTCCAGC TGGTCGTGAT GGCATCCAAG GCCCAGTTGG TCTCCCAGGC 
CCTGCCGGAC CTCCAGGTAT TTCTGGAGAA GATGGAGACA AGGGTGAGGT TGGAGAGCCA 
GGACAAAAGG GAGCTAAAGG GTCCAAAGGA GAACATGGTC CTCCTGGTCC ACCTGGGCCG 
ATGGGTCCTG TTGGACAGCC TGGTGCAGCG GGTGCTGATG GTGAGACGGG TCCAAGAGGT 
CAACAGGGAC AGTTTGGTGC CAAGGGAGAT GAAGGCACAA GAGGTTTCCC TGGTCCCCCT 
GGCCCCATAG GATTGCAGGG TTTGCCAGGC CCAGGTGGAG AAAAGGGAGA GACAGGAGAT 
GTTGGACCCT TGGGTCCTCC TGGTCCTCCA GGGCCGCGTG GTCCTGCTGG TCCTAATGGT 
GCTGATGGTC CCCAAGGTCC TCCAGGAGGT TTGGGTAATC CAGGTCCCAT AGGAGAGAAG 
GGTGAGCCTG GTGAATCTGG ACCACCTGGT GTTGGTGGAG AGCCAGGAAA ATTGGGCCCA 
AGAGGAGAGC GTGGTGAGAA GGGAGAGTCC GGGCAGCCTG GCACCCCTGG TCCTCCTGGA 
GGAAAGGGAC CCACTGGAGA TGACGGACCC AAAGGAAACC CTGGTCCTGT TGGATTCCCT 
GGTGATCCTG GACCCCCTGG TGAAGTTGGA CCACGAGGTC AAGATGGTGC AAAGGGTGAC 
AGAGGAGAGG ATGGAGAACA AGGAGAAGCC GGCTCACCTG GACCTACTGG AGAGAACGGA 
CCACCTGGAC CTCCAGGAAA GAGAGGTCCT GCAGGCACAA GGGGACCGGA GGGCCGTCAA 
GGAGAGAAAG GAAGCAAGGG TGATTCAGGT GCTCTTGGTC CTCCCGGAAA AACTGGCCCT 
GTGGGGCCAC AGGGACAACC CGGAAAACCA GGAACTGAGG GTCTAAGAGG GTTGCCTGGA 
ACAGTGGGTG AGCAAGGTTC TCCAGGTGCT GCAGGCCCTA AAGGACCTCC TGGACCTCTG 
GGTCCTCCAG GTCTGGCTGG TCTTCGCGGT GATCCCGGTG CTAAAGGTGA GAAGGGACAT 
CCAGGTATGC TCGGACTGAT CGGGCCTGCT GGAGAACAAG GAGAGAAGGG AGACAGAGGC 
ATGCCAGGAC CACAGGGATC AACAGGACCC AAGGGAGAAA CGGGTATATC TGGAGGAACT 
GGTCCACTTG GTCCTGCAGG TCCTGCTGGT ATGCCTGGCC CGAGGGGTGT CAAAGGAGCT 
AAAGGTGCCA GTGGTGGGGC TGGTCCTAAA GGAGAAAAGG GTGTACAAGG ACCACCAGGA 
CCACCTGGTC CCCCTGGTGA TGTCATCCAG CCGCTGCCCA TTCAGATCCC CAAGAAGTCC 
AAGCGCTCCA TCGACGCCAG TAAGATTCTG TCTGAGGCTG ACGCTGAGGC TGCCGACACC 
ACTGGCTCTG AGTTCCTGAC TGGTAATGAA GGAATGGAGG AGATCTTCGG CTCACTTAAT 
TCCCTGCGCC AGGAGATCGA GAGCATGCGC TTCCCATTGG GCACAAAGGA GAGCCCCGGC 
CGCACCTGCC AGGATCTCCA TCTCAGCCAA CCAGATCTGA AAGACGGCGA GTATTGGATT 
GATCCAAATC AAGGCTGTGC TCGCGACTCT TTCAAGGTTT ACTGTAACTT CACTGCCGGC 
GGAGAGACCT GCCTGTATCC CAGCAAAGCA GTGGAGAATG TAAAGATGAA CTCTTGGATA 
AAGGAGAAGC CTGGGTCCTT GTTCAGTCAG TTTGCAAAGG GCAACAAGTT TTCATATGTA 
GACTCGGTTG GAGAGGCTGT GGGTGTTGTT CAGCTGGGCT TCTTGCGGCT GCTCAGTGTC 
CAGGCCAGAC AGAACCTCAC CTACCACTGC CAACGCTCGG TGGCCTGGAC TGACCAAACC 
ACCGACGGCG GATACAAGCG GGCGCTGCGT CTGCAGGGGG CCAATGATGA GGAGATCAGC 
TATGAGACTA GCCCATATAT CAAAGCCCTC ATCGACGGCT GCTCCTATCG TAAGGGTCTG 
GACAGGACGG TGCTGGAGGT GAACACACCT CAGGTGGAGC AGCTCCCTCT GCTGGACATC 
AGGATCTCAG ACTTTGGGGA GGACAATCAG AAATTTGGTT TTGAAGTAGG ACCTGTCTGT 
TTCCTGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_017352253.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207742.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.

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CloneID	ODa23892
Clone ID Related Accession (Same CDS sequence)	NM_001328265.1 , XM_017352253.1 , XM_017352253.2
Accession Version	XM_017352253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCACTTTGAT GAAGAGCAGG ATGATTATGA ATACCCCTAT 
TATTATGAAG GAACTACCAG TGTCCCTCCC TCCCCATCCA AACCTCCCTC CGTCCCAGCC 
GAGACACTCA CTGAACAGGA CGTAGATCCT GCTTACTCAG AGGCTGACCA CACCCCCACA 
GAGACCGATT ATTATTACAC AGTAGAAGAA GAGGCGGCTC CACAGCCAGA GAGTGATCTG 
ACTGGATATG ACGAAAGCAT TAACCAGGTC GACGAGACTG TCCCCGTTGA GGAAATGGAT 
GCTACCAAGA CAGGTGGTGA GGTGGCGTCT CAAACGCCAG AAGAGCCCTT CACTGAGGAG 
TACATTACAA GCGACTTTGG GATGAAGGAG TATGACTATT CCTACAAGGA CTACAATGAG 
CCCATCATTG AGAGCAAGAC GGAGGACACT CTAGGCCCTG CCCTGTCCGC TGTGACAGAG 
GAGGGGGGTG CCTCAGTGCG AGCCATTAAG GGAGAGAAGG GAGAGCCTGC TGTCCTTGAA 
CCTGGCATGC TGATTGAAGG ACCACCAGGA CCAGAAGGAC CAGCAGGTCT CCCTGGCCCC 
TCTGGCCCCC AAGGACAACC TGGTTCTGTT GGTGATCCTG GTGAGAGGGG TCCTACTGGT 
AAGGCTGGTC TACCTGGAGC AGATGGTGTT CCAGGACCTC CAGGAACCTC TGTCATGCTT 
CCTTTCCGCT TTGGACAGAG CGCTGGAGAG AAAGGACCTG TTGCCTCTGC ACAGGAAGCC 
CAAGCTCAAG CCATTCTGTC TCAAGCTCGG CTTGCCCTGA AAGGACCATC CGGGCCAATG 
GGTTTCACTG GCCGTCCTGG ACCTTTGGGA ACACCTGGAA GTCCTGGCTT TAAGGGAGAA 
AGGGGGGACC CTGGTGCTCA GGGTCCTAGA GGTCCTCAGG GTGTGTCTGG TCCTCCTGGC 
AAAGCTGGTC GGAGGGGTCG CGCTGGTGCT GATGGAGCCA GAGGGATGCC AGGAGAGTCT 
GGAATTAAGG GTGACCGTGG TTTTGATGGC CTTCCTGGTT TGCCTGGTGA CAAGGGTTAT 
CGGGGTCAAC CTGGAGGAAT GGGACCCCCA GGACCACATG GAGAGGACGG AGAGAGGGGA 
GATGATGGAG ATGTTGGACC CAGAGGTCTT CCTGGCGAAC CTGGACCCCG TGGTTTGTTG 
GGGCCTAAAG GGCCTTCTGG ACTTCCTGGA CCTCCTGGTG TTCGTGGAAA TGATGGTCCA 
CATGGTCCAA AAGGAAACTT AGGACCACAA GGAGAGCCCG GTCCTCCAGG CCAGCAGGGG 
GCTCCAGGAA CACAGGGAAT GCCAGGTCCA CAGGGTGCCA CTGGACCACC AGGAGAGAAA 
GGACCCACAG GAAAGCCAGG TCTACCAGGA ATGCCAGGAG CTGATGGTCC TCCCGGTCAT 
CCCGGGAAGG AAGGTCCTGG TGGAACCAAA GGAAATCAAG GTCCTAATGG TCCTCAGGGC 
TCTATTGGCT ATCCTGGCCC TCGGGGATTG AAGGGAGCTC AAGGTATCCG TGGACTGAAA 
GGTCATAAAG GAGAGAAGGG AGAGGATGGA TTCCCAGGAA TTAAAGGAGA CTTTGGTGTG 
AAGGGAGAGA GGGGAGAGGT GGGCGTGCCC GGCCCAAGAG GAGAAGATGG TCCTGAGGGG 
CCAAAGGGTC GCGTCGGACC CCCTGGTGAA ATTGGTCCCC TCGGCCTGTT GGGAGAGAAG 
GGTAAACTTG GTGTTCCTGG ACTTCCTGGA TATCCTGGAC GTCAAGGAAT AAAGGGGTCC 
CTTGGTTTCC CAGGATTCCC TGGTACAAAT GGCGAGAAGG GCACAAGGGG TTTGATTGGA 
AAACCTGGAC CAAGAGGACA AAGAGGGCCG ACAGGTCCTA GAGGGCAGAG AGGACCACGT 
GGGGCAACTG GGAAATCAGG TGCTAAGGGT GGATCAGGAA GTGACGGGCC TCCTGGACCA 
CCAGGAGAGA GAGGGCTAAC TGGACCTCAG GGTGCTAATG GATTCCCTGG GCCAAAGGGA 
CCTCCCGGAC CTCCAGGGAA AGATGGACTT CCTGGACACC CTGGACAGAG AGGCGAAGTC 
GGTTTCCAAG GCAAAGTTGG ACCACCTGGC CCACCTGGAG TTGTTGGCCC ACACGGTCCC 
TCTGGTGAGA CTGGTCAGAT GGGTGAGCGT GGCCACCCTG GACCTCCAGG ACCACCTGGT 
GAGCAAGGCC TTTCTGGCTC CTCTGGTAAA GAAGGAACCA AAGGAGATCC CGGTCCTCCT 
GGTGGCCCCG GTAAAGATGG ACCCCCTGGA CTTAGAGGAT TCCCAGGAGA GAGAGGACTA 
CCAGGAACTC CAGGTTCTGG AGGACTGAAA GGAAATGAAG GCCCTGCTGG ACCACCTGGT 
CCTGCGGGTT CATCTGGGGA GAGAGGTGGT GCTGGCACTG CAGGCCCTGT CGGCCCACCT 
GGACGACCTG GACCTCAGGG ACCCCCTGGA ACTTCAGGTG AAAAGGGAGT ACCAGGCGAG 
AAAGGTCCGG TTGGTCCAGC TGGTCGTGAT GGCATCCAAG GCCCAGTTGG TCTCCCAGGC 
CCTGCCGGAC CTCCAGGTAT TTCTGGAGAA GATGGAGACA AGGGTGAGGT TGGAGAGCCA 
GGACAAAAGG GAGCTAAAGG GTCCAAAGGA GAACATGGTC CTCCTGGTCC ACCTGGGCCG 
ATGGGTCCTG TTGGACAGCC TGGTGCAGCG GGTGCTGATG GTGAGACGGG TCCAAGAGGT 
CAACAGGGAC AGTTTGGTGC CAAGGGAGAT GAAGGCACAA GAGGTTTCCC TGGTCCCCCT 
GGCCCCATAG GATTGCAGGG TTTGCCAGGC CCAGGTGGAG AAAAGGGAGA GACAGGAGAT 
GTTGGACCCT TGGGTCCTCC TGGTCCTCCA GGGCCGCGTG GTCCTGCTGG TCCTAATGGT 
GCTGATGGTC CCCAAGGTCC TCCAGGAGGT TTGGGTAATC CAGGTCCCAT AGGAGAGAAG 
GGTGAGCCTG GTGAATCTGG ACCACCTGGT GTTGGTGGAG AGCCAGGAAA ATTGGGCCCA 
AGAGGAGAGC GTGGTGAGAA GGGAGAGTCC GGGCAGCCTG GCACCCCTGG TCCTCCTGGA 
GGAAAGGGAC CCACTGGAGA TGACGGACCC AAAGGAAACC CTGGTCCTGT TGGATTCCCT 
GGTGATCCTG GACCCCCTGG TGAAGTTGGA CCACGAGGTC AAGATGGTGC AAAGGGTGAC 
AGAGGAGAGG ATGGAGAACA AGGAGAAGCC GGCTCACCTG GACCTACTGG AGAGAACGGA 
CCACCTGGAC CTCCAGGAAA GAGAGGTCCT GCAGGCACAA GGGGACCGGA GGGCCGTCAA 
GGAGAGAAAG GAAGCAAGGG TGATTCAGGT GCTCTTGGTC CTCCCGGAAA AACTGGCCCT 
GTGGGGCCAC AGGGACAACC CGGAAAACCA GGAACTGAGG GTCTAAGAGG GTTGCCTGGA 
ACAGTGGGTG AGCAAGGTTC TCCAGGTGCT GCAGGCCCTA AAGGACCTCC TGGACCTCTG 
GGTCCTCCAG GTCTGGCTGG TCTTCGCGGT GATCCCGGTG CTAAAGGTGA GAAGGGACAT 
CCAGGTATGC TCGGACTGAT CGGGCCTGCT GGAGAACAAG GAGAGAAGGG AGACAGAGGC 
ATGCCAGGAC CACAGGGATC AACAGGACCC AAGGGAGAAA CGGGTATATC TGGAGGAACT 
GGTCCACTTG GTCCTGCAGG TCCTGCTGGT ATGCCTGGCC CGAGGGGTGT CAAAGGAGCT 
AAAGGTGCCA GTGGTGGGGC TGGTCCTAAA GGAGAAAAGG GTGTACAAGG ACCACCAGGA 
CCACCTGGTC CCCCTGGTGA TGTCATCCAG CCGCTGCCCA TTCAGATCCC CAAGAAGTCC 
AAGCGCTCCA TCGACGCCAG TAAGATTCTG TCTGAGGCTG ACGCTGAGGC TGCCGACACC 
ACTGGCTCTG AGTTCCTGAC TGGTAATGAA GGAATGGAGG AGATCTTCGG CTCACTTAAT 
TCCCTGCGCC AGGAGATCGA GAGCATGCGC TTCCCATTGG GCACAAAGGA GAGCCCCGGC 
CGCACCTGCC AGGATCTCCA TCTCAGCCAA CCAGATCTGA AAGACGGCGA GTATTGGATT 
GATCCAAATC AAGGCTGTGC TCGCGACTCT TTCAAGGTTT ACTGTAACTT CACTGCCGGC 
GGAGAGACCT GCCTGTATCC CAGCAAAGCA GTGGAGAATG TAAAGATGAA CTCTTGGATA 
AAGGAGAAGC CTGGGTCCTT GTTCAGTCAG TTTGCAAAGG GCAACAAGTT TTCATATGTA 
GACTCGGTTG GAGAGGCTGT GGGTGTTGTT CAGCTGGGCT TCTTGCGGCT GCTCAGTGTC 
CAGGCCAGAC AGAACCTCAC CTACCACTGC CAACGCTCGG TGGCCTGGAC TGACCAAACC 
ACCGACGGCG GATACAAGCG GGCGCTGCGT CTGCAGGGGG CCAATGATGA GGAGATCAGC 
TATGAGACTA GCCCATATAT CAAAGCCCTC ATCGACGGCT GCTCCTATCG TAAGGGTCTG 
GACAGGACGG TGCTGGAGGT GAACACACCT CAGGTGGAGC AGCTCCCTCT GCTGGACATC 
AGGATCTCAG ACTTTGGGGA GGACAATCAG AAATTTGGTT TTGAAGTAGG ACCTGTCTGT 
TTCCTGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207742.1
CDS	393..5804
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYHFDEEQDDYEYPYYYEGTTSVPPSPSKPPSVPAETLTEQDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_017352253.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352253.2

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCACTTTGAT GAAGAGCAGG ATGATTATGA ATACCCCTAT 
TATTATGAAG GAACTACCAG TGTCCCTCCC TCCCCATCCA AACCTCCCTC CGTCCCAGCC 
GAGACACTCA CTGAACAGGA CGTAGATCCT GCTTACTCAG AGGCTGACCA CACCCCCACA 
GAGACCGATT ATTATTACAC AGTAGAAGAA GAGGCGGCTC CACAGCCAGA GAGTGATCTG 
ACTGGATATG ACGAAAGCAT TAACCAGGTC GACGAGACTG TCCCCGTTGA GGAAATGGAT 
GCTACCAAGA CAGGTGGTGA GGTGGCGTCT CAAACGCCAG AAGAGCCCTT CACTGAGGAG 
TACATTACAA GCGACTTTGG GATGAAGGAG TATGACTATT CCTACAAGGA CTACAATGAG 
CCCATCATTG AGAGCAAGAC GGAGGACACT CTAGGCCCTG CCCTGTCCGC TGTGACAGAG 
GAGGGGGGTG CCTCAGTGCG AGCCATTAAG GGAGAGAAGG GAGAGCCTGC TGTCCTTGAA 
CCTGGCATGC TGATTGAAGG ACCACCAGGA CCAGAAGGAC CAGCAGGTCT CCCTGGCCCC 
TCTGGCCCCC AAGGACAACC TGGTTCTGTT GGTGATCCTG GTGAGAGGGG TCCTACTGGT 
AAGGCTGGTC TACCTGGAGC AGATGGTGTT CCAGGACCTC CAGGAACCTC TGTCATGCTT 
CCTTTCCGCT TTGGACAGAG CGCTGGAGAG AAAGGACCTG TTGCCTCTGC ACAGGAAGCC 
CAAGCTCAAG CCATTCTGTC TCAAGCTCGG CTTGCCCTGA AAGGACCATC CGGGCCAATG 
GGTTTCACTG GCCGTCCTGG ACCTTTGGGA ACACCTGGAA GTCCTGGCTT TAAGGGAGAA 
AGGGGGGACC CTGGTGCTCA GGGTCCTAGA GGTCCTCAGG GTGTGTCTGG TCCTCCTGGC 
AAAGCTGGTC GGAGGGGTCG CGCTGGTGCT GATGGAGCCA GAGGGATGCC AGGAGAGTCT 
GGAATTAAGG GTGACCGTGG TTTTGATGGC CTTCCTGGTT TGCCTGGTGA CAAGGGTTAT 
CGGGGTCAAC CTGGAGGAAT GGGACCCCCA GGACCACATG GAGAGGACGG AGAGAGGGGA 
GATGATGGAG ATGTTGGACC CAGAGGTCTT CCTGGCGAAC CTGGACCCCG TGGTTTGTTG 
GGGCCTAAAG GGCCTTCTGG ACTTCCTGGA CCTCCTGGTG TTCGTGGAAA TGATGGTCCA 
CATGGTCCAA AAGGAAACTT AGGACCACAA GGAGAGCCCG GTCCTCCAGG CCAGCAGGGG 
GCTCCAGGAA CACAGGGAAT GCCAGGTCCA CAGGGTGCCA CTGGACCACC AGGAGAGAAA 
GGACCCACAG GAAAGCCAGG TCTACCAGGA ATGCCAGGAG CTGATGGTCC TCCCGGTCAT 
CCCGGGAAGG AAGGTCCTGG TGGAACCAAA GGAAATCAAG GTCCTAATGG TCCTCAGGGC 
TCTATTGGCT ATCCTGGCCC TCGGGGATTG AAGGGAGCTC AAGGTATCCG TGGACTGAAA 
GGTCATAAAG GAGAGAAGGG AGAGGATGGA TTCCCAGGAA TTAAAGGAGA CTTTGGTGTG 
AAGGGAGAGA GGGGAGAGGT GGGCGTGCCC GGCCCAAGAG GAGAAGATGG TCCTGAGGGG 
CCAAAGGGTC GCGTCGGACC CCCTGGTGAA ATTGGTCCCC TCGGCCTGTT GGGAGAGAAG 
GGTAAACTTG GTGTTCCTGG ACTTCCTGGA TATCCTGGAC GTCAAGGAAT AAAGGGGTCC 
CTTGGTTTCC CAGGATTCCC TGGTACAAAT GGCGAGAAGG GCACAAGGGG TTTGATTGGA 
AAACCTGGAC CAAGAGGACA AAGAGGGCCG ACAGGTCCTA GAGGGCAGAG AGGACCACGT 
GGGGCAACTG GGAAATCAGG TGCTAAGGGT GGATCAGGAA GTGACGGGCC TCCTGGACCA 
CCAGGAGAGA GAGGGCTAAC TGGACCTCAG GGTGCTAATG GATTCCCTGG GCCAAAGGGA 
CCTCCCGGAC CTCCAGGGAA AGATGGACTT CCTGGACACC CTGGACAGAG AGGCGAAGTC 
GGTTTCCAAG GCAAAGTTGG ACCACCTGGC CCACCTGGAG TTGTTGGCCC ACACGGTCCC 
TCTGGTGAGA CTGGTCAGAT GGGTGAGCGT GGCCACCCTG GACCTCCAGG ACCACCTGGT 
GAGCAAGGCC TTTCTGGCTC CTCTGGTAAA GAAGGAACCA AAGGAGATCC CGGTCCTCCT 
GGTGGCCCCG GTAAAGATGG ACCCCCTGGA CTTAGAGGAT TCCCAGGAGA GAGAGGACTA 
CCAGGAACTC CAGGTTCTGG AGGACTGAAA GGAAATGAAG GCCCTGCTGG ACCACCTGGT 
CCTGCGGGTT CATCTGGGGA GAGAGGTGGT GCTGGCACTG CAGGCCCTGT CGGCCCACCT 
GGACGACCTG GACCTCAGGG ACCCCCTGGA ACTTCAGGTG AAAAGGGAGT ACCAGGCGAG 
AAAGGTCCGG TTGGTCCAGC TGGTCGTGAT GGCATCCAAG GCCCAGTTGG TCTCCCAGGC 
CCTGCCGGAC CTCCAGGTAT TTCTGGAGAA GATGGAGACA AGGGTGAGGT TGGAGAGCCA 
GGACAAAAGG GAGCTAAAGG GTCCAAAGGA GAACATGGTC CTCCTGGTCC ACCTGGGCCG 
ATGGGTCCTG TTGGACAGCC TGGTGCAGCG GGTGCTGATG GTGAGACGGG TCCAAGAGGT 
CAACAGGGAC AGTTTGGTGC CAAGGGAGAT GAAGGCACAA GAGGTTTCCC TGGTCCCCCT 
GGCCCCATAG GATTGCAGGG TTTGCCAGGC CCAGGTGGAG AAAAGGGAGA GACAGGAGAT 
GTTGGACCCT TGGGTCCTCC TGGTCCTCCA GGGCCGCGTG GTCCTGCTGG TCCTAATGGT 
GCTGATGGTC CCCAAGGTCC TCCAGGAGGT TTGGGTAATC CAGGTCCCAT AGGAGAGAAG 
GGTGAGCCTG GTGAATCTGG ACCACCTGGT GTTGGTGGAG AGCCAGGAAA ATTGGGCCCA 
AGAGGAGAGC GTGGTGAGAA GGGAGAGTCC GGGCAGCCTG GCACCCCTGG TCCTCCTGGA 
GGAAAGGGAC CCACTGGAGA TGACGGACCC AAAGGAAACC CTGGTCCTGT TGGATTCCCT 
GGTGATCCTG GACCCCCTGG TGAAGTTGGA CCACGAGGTC AAGATGGTGC AAAGGGTGAC 
AGAGGAGAGG ATGGAGAACA AGGAGAAGCC GGCTCACCTG GACCTACTGG AGAGAACGGA 
CCACCTGGAC CTCCAGGAAA GAGAGGTCCT GCAGGCACAA GGGGACCGGA GGGCCGTCAA 
GGAGAGAAAG GAAGCAAGGG TGATTCAGGT GCTCTTGGTC CTCCCGGAAA AACTGGCCCT 
GTGGGGCCAC AGGGACAACC CGGAAAACCA GGAACTGAGG GTCTAAGAGG GTTGCCTGGA 
ACAGTGGGTG AGCAAGGTTC TCCAGGTGCT GCAGGCCCTA AAGGACCTCC TGGACCTCTG 
GGTCCTCCAG GTCTGGCTGG TCTTCGCGGT GATCCCGGTG CTAAAGGTGA GAAGGGACAT 
CCAGGTATGC TCGGACTGAT CGGGCCTGCT GGAGAACAAG GAGAGAAGGG AGACAGAGGC 
ATGCCAGGAC CACAGGGATC AACAGGACCC AAGGGAGAAA CGGGTATATC TGGAGGAACT 
GGTCCACTTG GTCCTGCAGG TCCTGCTGGT ATGCCTGGCC CGAGGGGTGT CAAAGGAGCT 
AAAGGTGCCA GTGGTGGGGC TGGTCCTAAA GGAGAAAAGG GTGTACAAGG ACCACCAGGA 
CCACCTGGTC CCCCTGGTGA TGTCATCCAG CCGCTGCCCA TTCAGATCCC CAAGAAGTCC 
AAGCGCTCCA TCGACGCCAG TAAGATTCTG TCTGAGGCTG ACGCTGAGGC TGCCGACACC 
ACTGGCTCTG AGTTCCTGAC TGGTAATGAA GGAATGGAGG AGATCTTCGG CTCACTTAAT 
TCCCTGCGCC AGGAGATCGA GAGCATGCGC TTCCCATTGG GCACAAAGGA GAGCCCCGGC 
CGCACCTGCC AGGATCTCCA TCTCAGCCAA CCAGATCTGA AAGACGGCGA GTATTGGATT 
GATCCAAATC AAGGCTGTGC TCGCGACTCT TTCAAGGTTT ACTGTAACTT CACTGCCGGC 
GGAGAGACCT GCCTGTATCC CAGCAAAGCA GTGGAGAATG TAAAGATGAA CTCTTGGATA 
AAGGAGAAGC CTGGGTCCTT GTTCAGTCAG TTTGCAAAGG GCAACAAGTT TTCATATGTA 
GACTCGGTTG GAGAGGCTGT GGGTGTTGTT CAGCTGGGCT TCTTGCGGCT GCTCAGTGTC 
CAGGCCAGAC AGAACCTCAC CTACCACTGC CAACGCTCGG TGGCCTGGAC TGACCAAACC 
ACCGACGGCG GATACAAGCG GGCGCTGCGT CTGCAGGGGG CCAATGATGA GGAGATCAGC 
TATGAGACTA GCCCATATAT CAAAGCCCTC ATCGACGGCT GCTCCTATCG TAAGGGTCTG 
GACAGGACGG TGCTGGAGGT GAACACACCT CAGGTGGAGC AGCTCCCTCT GCTGGACATC 
AGGATCTCAG ACTTTGGGGA GGACAATCAG AAATTTGGTT TTGAAGTAGG ACCTGTCTGT 
TTCCTGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159448.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159505.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.

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CloneID	ODa23893
Clone ID Related Accession (Same CDS sequence)	XM_005159448.4 , XM_005159448.3
Accession Version	XM_005159448.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159448.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGACGTAGAT CCTGCTTACT CAGAGGCTGA CCACACCCCC 
ACAGAGACCG ATTATTATTA CACAGTAGAA GAAGAGGCGG CTCCACAGCC AGAGAGTGAT 
CTGACTGGAT ATGACGAAAG CATTAACCAG GTCGACGAGA CTGTCCCCGT TGAGGAAATG 
GATGCTACCA AGACAGGTGG TGAGGTGGCG TCTCAAACGC CAGAAGAGCC CTTCACTGAG 
GAGTACATTA CAAGCGACTT TGGGATGAAG GAGTATGACT ATTCCTACAA GGACTACAAT 
GAGCCCATCA TTGAGAGCAA GACGGAGGAC ACTCTAGGCC CTGCCCTGTC CGCTGTGACA 
GAGGAGGGGG GTGCCTCAGT GCGAGCCATT AAGGGAGAGA AGGGAGAGCC TGCTGTCCTT 
GAACCTGGCA TGCTGATTGA AGGACCACCA GGACCAGAAG GACCAGCAGG TCTCCCTGGC 
CCCTCTGGCC CCCAAGGACA ACCTGGTTCT GTTGGTGATC CTGGTGAGAG GGGTCCTACT 
GGTAAGGCTG GTCTACCTGG AGCAGATGGT GTTCCAGGAC CTCCAGGAAC CTCTGTCATG 
CTTCCTTTCC GCTTTGGACA GAGCGCTGGA GAGAAAGGAC CTGTTGCCTC TGCACAGGAA 
GCCCAAGCTC AAGCCATTCT GTCTCAAGCT CGGCTTGCCC TGAAAGGACC ATCCGGGCCA 
ATGGGTTTCA CTGGCCGTCC TGGACCTTTG GGAACACCTG GAAGTCCTGG CTTTAAGGGA 
GAAAGGGGGG ACCCTGGTGC TCAGGGTCCT AGAGGTCCTC AGGGTGTGTC TGGTCCTCCT 
GGCAAAGCTG GTCGGAGGGG TCGCGCTGGT GCTGATGGAG CCAGAGGGAT GCCAGGAGAG 
TCTGGAATTA AGGGTGACCG TGGTTTTGAT GGCCTTCCTG GTTTGCCTGG TGACAAGGGT 
TATCGGGGTC AACCTGGAGG AATGGGACCC CCAGGACCAC ATGGAGAGGA CGGAGAGAGG 
GGAGATGATG GAGATGTTGG ACCCAGAGGT CTTCCTGGCG AACCTGGACC CCGTGGTTTG 
TTGGGGCCTA AAGGGCCTTC TGGACTTCCT GGACCTCCTG GTGTTCGTGG AAATGATGGT 
CCACATGGTC CAAAAGGAAA CTTAGGACCA CAAGGAGAGC CCGGTCCTCC AGGCCAGCAG 
GGGGCTCCAG GAACACAGGG AATGCCAGGT CCACAGGGTG CCACTGGACC ACCAGGAGAG 
AAAGGACCCA CAGGAAAGCC AGGTCTACCA GGAATGCCAG GAGCTGATGG TCCTCCCGGT 
CATCCCGGGA AGGAAGGTCC TGGTGGAACC AAAGGAAATC AAGGTCCTAA TGGTCCTCAG 
GGCTCTATTG GCTATCCTGG CCCTCGGGGA TTGAAGGGAG CTCAAGGTAT CCGTGGACTG 
AAAGGTCATA AAGGAGAGAA GGGAGAGGAT GGATTCCCAG GAATTAAAGG AGACTTTGGT 
GTGAAGGGAG AGAGGGGAGA GGTGGGCGTG CCCGGCCCAA GAGGAGAAGA TGGTCCTGAG 
GGGCCAAAGG GTCGCGTCGG ACCCCCTGGT GAAATTGGTC CCCTCGGCCT GTTGGGAGAG 
AAGGGTAAAC TTGGTGTTCC TGGACTTCCT GGATATCCTG GACGTCAAGG AATAAAGGGG 
TCCCTTGGTT TCCCAGGATT CCCTGGTACA AATGGCGAGA AGGGCACAAG GGGTTTGATT 
GGAAAACCTG GACCAAGAGG ACAAAGAGGG CCGACAGGTC CTAGAGGGCA GAGAGGACCA 
CGTGGGGCAA CTGGGAAATC AGGTGCTAAG GGTGGATCAG GAAGTGACGG GCCTCCTGGA 
CCACCAGGAG AGAGAGGGCT AACTGGACCT CAGGGTGCTA ATGGATTCCC TGGGCCAAAG 
GGACCTCCCG GACCTCCAGG GAAAGATGGA CTTCCTGGAC ACCCTGGACA GAGAGGCGAA 
GTCGGTTTCC AAGGCAAAGT TGGACCACCT GGCCCACCTG GAGTTGTTGG CCCACACGGT 
CCCTCTGGTG AGACTGGTCA GATGGGTGAG CGTGGCCACC CTGGACCTCC AGGACCACCT 
GGTGAGCAAG GCCTTTCTGG CTCCTCTGGT AAAGAAGGAA CCAAAGGAGA TCCCGGTCCT 
CCTGGTGGCC CCGGTAAAGA TGGACCCCCT GGACTTAGAG GATTCCCAGG AGAGAGAGGA 
CTACCAGGAA CTCCAGGTTC TGGAGGACTG AAAGGAAATG AAGGCCCTGC TGGACCACCT 
GGTCCTGCGG GTTCATCTGG GGAGAGAGGT GGTGCTGGCA CTGCAGGCCC TGTCGGCCCA 
CCTGGACGAC CTGGACCTCA GGGACCCCCT GGAACTTCAG GTGAAAAGGG AGTACCAGGC 
GAGAAAGGTC CGGTTGGTCC AGCTGGTCGT GATGGCATCC AAGGCCCAGT TGGTCTCCCA 
GGCCCTGCCG GACCTCCAGG TATTTCTGGA GAAGATGGAG ACAAGGGTGA GGTTGGAGAG 
CCAGGACAAA AGGGAGCTAA AGGGTCCAAA GGAGAACATG GTCCTCCTGG TCCACCTGGG 
CCGATGGGTC CTGTTGGACA GCCTGGTGCA GCGGGTGCTG ATGGTGAGAC GGGTCCAAGA 
GGTCAACAGG GACAGTTTGG TGCCAAGGGA GATGAAGGCA CAAGAGGTTT CCCTGGTCCC 
CCTGGCCCCA TAGGATTGCA GGGTTTGCCA GGCCCAGGTG GAGAAAAGGG AGAGACAGGA 
GATGTTGGAC CCTTGGGTCC TCCTGGTCCT CCAGGGCCGC GTGGTCCTGC TGGTCCTAAT 
GGTGCTGATG GTCCCCAAGG TCCTCCAGGA GGTTTGGGTA ATCCAGGTCC CATAGGAGAG 
AAGGGTGAGC CTGGTGAATC TGGACCACCT GGTGTTGGTG GAGAGCCAGG AAAATTGGGC 
CCAAGAGGAG AGCGTGGTGA GAAGGGAGAG TCCGGGCAGC CTGGCACCCC TGGTCCTCCT 
GGAGGAAAGG GACCCACTGG AGATGACGGA CCCAAAGGAA ACCCTGGTCC TGTTGGATTC 
CCTGGTGATC CTGGACCCCC TGGTGAAGTT GGACCACGAG GTCAAGATGG TGCAAAGGGT 
GACAGAGGAG AGGATGGAGA ACAAGGAGAA GCCGGCTCAC CTGGACCTAC TGGAGAGAAC 
GGACCACCTG GACCTCCAGG AAAGAGAGGT CCTGCAGGCA CAAGGGGACC GGAGGGCCGT 
CAAGGAGAGA AAGGAAGCAA GGGTGATTCA GGTGCTCTTG GTCCTCCCGG AAAAACTGGC 
CCTGTGGGGC CACAGGGACA ACCCGGAAAA CCAGGAACTG AGGGTCTAAG AGGGTTGCCT 
GGAACAGTGG GTGAGCAAGG TTCTCCAGGT GCTGCAGGCC CTAAAGGACC TCCTGGACCT 
CTGGGTCCTC CAGGTCTGGC TGGTCTTCGC GGTGATCCCG GTGCTAAAGG TGAGAAGGGA 
CATCCAGGTA TGCTCGGACT GATCGGGCCT GCTGGAGAAC AAGGAGAGAA GGGAGACAGA 
GGCATGCCAG GACCACAGGG ATCAACAGGA CCCAAGGGAG AAACGGGTAT ATCTGGAGGA 
ACTGGTCCAC TTGGTCCTGC AGGTCCTGCT GGTATGCCTG GCCCGAGGGG TGTCAAAGGA 
GCTAAAGGTG CCAGTGGTGG GGCTGGTCCT AAAGGAGAAA AGGGTGTACA AGGACCACCA 
GGACCACCTG GTCCCCCTGG TGATGTCATC CAGCCGCTGC CCATTCAGAT CCCCAAGAAG 
TCCAAGCGCT CCATCGACGC CAGTAAGATT CTGTCTGAGG CTGACGCTGA GGCTGCCGAC 
ACCACTGGCT CTGAGTTCCT GACTGGTAAT GAAGGAATGG AGGAGATCTT CGGCTCACTT 
AATTCCCTGC GCCAGGAGAT CGAGAGCATG CGCTTCCCAT TGGGCACAAA GGAGAGCCCC 
GGCCGCACCT GCCAGGATCT CCATCTCAGC CAACCAGATC TGAAAGACGG CGAGTATTGG 
ATTGATCCAA ATCAAGGCTG TGCTCGCGAC TCTTTCAAGG TTTACTGTAA CTTCACTGCC 
GGCGGAGAGA CCTGCCTGTA TCCCAGCAAA GCAGTGGAGA ATGTAAAGAT GAACTCTTGG 
ATAAAGGAGA AGCCTGGGTC CTTGTTCAGT CAGTTTGCAA AGGGCAACAA GTTTTCATAT 
GTAGACTCGG TTGGAGAGGC TGTGGGTGTT GTTCAGCTGG GCTTCTTGCG GCTGCTCAGT 
GTCCAGGCCA GACAGAACCT CACCTACCAC TGCCAACGCT CGGTGGCCTG GACTGACCAA 
ACCACCGACG GCGGATACAA GCGGGCGCTG CGTCTGCAGG GGGCCAATGA TGAGGAGATC 
AGCTATGAGA CTAGCCCATA TATCAAAGCC CTCATCGACG GCTGCTCCTA TCGTAAGGGT 
CTGGACAGGA CGGTGCTGGA GGTGAACACA CCTCAGGTGG AGCAGCTCCC TCTGCTGGAC 
ATCAGGATCT CAGACTTTGG GGAGGACAAT CAGAAATTTG GTTTTGAAGT AGGACCTGTC 
TGTTTCCTGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159505.1
CDS	393..5687
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159448.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159448.4

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGACGTAGAT CCTGCTTACT CAGAGGCTGA CCACACCCCC 
ACAGAGACCG ATTATTATTA CACAGTAGAA GAAGAGGCGG CTCCACAGCC AGAGAGTGAT 
CTGACTGGAT ATGACGAAAG CATTAACCAG GTCGACGAGA CTGTCCCCGT TGAGGAAATG 
GATGCTACCA AGACAGGTGG TGAGGTGGCG TCTCAAACGC CAGAAGAGCC CTTCACTGAG 
GAGTACATTA CAAGCGACTT TGGGATGAAG GAGTATGACT ATTCCTACAA GGACTACAAT 
GAGCCCATCA TTGAGAGCAA GACGGAGGAC ACTCTAGGCC CTGCCCTGTC CGCTGTGACA 
GAGGAGGGGG GTGCCTCAGT GCGAGCCATT AAGGGAGAGA AGGGAGAGCC TGCTGTCCTT 
GAACCTGGCA TGCTGATTGA AGGACCACCA GGACCAGAAG GACCAGCAGG TCTCCCTGGC 
CCCTCTGGCC CCCAAGGACA ACCTGGTTCT GTTGGTGATC CTGGTGAGAG GGGTCCTACT 
GGTAAGGCTG GTCTACCTGG AGCAGATGGT GTTCCAGGAC CTCCAGGAAC CTCTGTCATG 
CTTCCTTTCC GCTTTGGACA GAGCGCTGGA GAGAAAGGAC CTGTTGCCTC TGCACAGGAA 
GCCCAAGCTC AAGCCATTCT GTCTCAAGCT CGGCTTGCCC TGAAAGGACC ATCCGGGCCA 
ATGGGTTTCA CTGGCCGTCC TGGACCTTTG GGAACACCTG GAAGTCCTGG CTTTAAGGGA 
GAAAGGGGGG ACCCTGGTGC TCAGGGTCCT AGAGGTCCTC AGGGTGTGTC TGGTCCTCCT 
GGCAAAGCTG GTCGGAGGGG TCGCGCTGGT GCTGATGGAG CCAGAGGGAT GCCAGGAGAG 
TCTGGAATTA AGGGTGACCG TGGTTTTGAT GGCCTTCCTG GTTTGCCTGG TGACAAGGGT 
TATCGGGGTC AACCTGGAGG AATGGGACCC CCAGGACCAC ATGGAGAGGA CGGAGAGAGG 
GGAGATGATG GAGATGTTGG ACCCAGAGGT CTTCCTGGCG AACCTGGACC CCGTGGTTTG 
TTGGGGCCTA AAGGGCCTTC TGGACTTCCT GGACCTCCTG GTGTTCGTGG AAATGATGGT 
CCACATGGTC CAAAAGGAAA CTTAGGACCA CAAGGAGAGC CCGGTCCTCC AGGCCAGCAG 
GGGGCTCCAG GAACACAGGG AATGCCAGGT CCACAGGGTG CCACTGGACC ACCAGGAGAG 
AAAGGACCCA CAGGAAAGCC AGGTCTACCA GGAATGCCAG GAGCTGATGG TCCTCCCGGT 
CATCCCGGGA AGGAAGGTCC TGGTGGAACC AAAGGAAATC AAGGTCCTAA TGGTCCTCAG 
GGCTCTATTG GCTATCCTGG CCCTCGGGGA TTGAAGGGAG CTCAAGGTAT CCGTGGACTG 
AAAGGTCATA AAGGAGAGAA GGGAGAGGAT GGATTCCCAG GAATTAAAGG AGACTTTGGT 
GTGAAGGGAG AGAGGGGAGA GGTGGGCGTG CCCGGCCCAA GAGGAGAAGA TGGTCCTGAG 
GGGCCAAAGG GTCGCGTCGG ACCCCCTGGT GAAATTGGTC CCCTCGGCCT GTTGGGAGAG 
AAGGGTAAAC TTGGTGTTCC TGGACTTCCT GGATATCCTG GACGTCAAGG AATAAAGGGG 
TCCCTTGGTT TCCCAGGATT CCCTGGTACA AATGGCGAGA AGGGCACAAG GGGTTTGATT 
GGAAAACCTG GACCAAGAGG ACAAAGAGGG CCGACAGGTC CTAGAGGGCA GAGAGGACCA 
CGTGGGGCAA CTGGGAAATC AGGTGCTAAG GGTGGATCAG GAAGTGACGG GCCTCCTGGA 
CCACCAGGAG AGAGAGGGCT AACTGGACCT CAGGGTGCTA ATGGATTCCC TGGGCCAAAG 
GGACCTCCCG GACCTCCAGG GAAAGATGGA CTTCCTGGAC ACCCTGGACA GAGAGGCGAA 
GTCGGTTTCC AAGGCAAAGT TGGACCACCT GGCCCACCTG GAGTTGTTGG CCCACACGGT 
CCCTCTGGTG AGACTGGTCA GATGGGTGAG CGTGGCCACC CTGGACCTCC AGGACCACCT 
GGTGAGCAAG GCCTTTCTGG CTCCTCTGGT AAAGAAGGAA CCAAAGGAGA TCCCGGTCCT 
CCTGGTGGCC CCGGTAAAGA TGGACCCCCT GGACTTAGAG GATTCCCAGG AGAGAGAGGA 
CTACCAGGAA CTCCAGGTTC TGGAGGACTG AAAGGAAATG AAGGCCCTGC TGGACCACCT 
GGTCCTGCGG GTTCATCTGG GGAGAGAGGT GGTGCTGGCA CTGCAGGCCC TGTCGGCCCA 
CCTGGACGAC CTGGACCTCA GGGACCCCCT GGAACTTCAG GTGAAAAGGG AGTACCAGGC 
GAGAAAGGTC CGGTTGGTCC AGCTGGTCGT GATGGCATCC AAGGCCCAGT TGGTCTCCCA 
GGCCCTGCCG GACCTCCAGG TATTTCTGGA GAAGATGGAG ACAAGGGTGA GGTTGGAGAG 
CCAGGACAAA AGGGAGCTAA AGGGTCCAAA GGAGAACATG GTCCTCCTGG TCCACCTGGG 
CCGATGGGTC CTGTTGGACA GCCTGGTGCA GCGGGTGCTG ATGGTGAGAC GGGTCCAAGA 
GGTCAACAGG GACAGTTTGG TGCCAAGGGA GATGAAGGCA CAAGAGGTTT CCCTGGTCCC 
CCTGGCCCCA TAGGATTGCA GGGTTTGCCA GGCCCAGGTG GAGAAAAGGG AGAGACAGGA 
GATGTTGGAC CCTTGGGTCC TCCTGGTCCT CCAGGGCCGC GTGGTCCTGC TGGTCCTAAT 
GGTGCTGATG GTCCCCAAGG TCCTCCAGGA GGTTTGGGTA ATCCAGGTCC CATAGGAGAG 
AAGGGTGAGC CTGGTGAATC TGGACCACCT GGTGTTGGTG GAGAGCCAGG AAAATTGGGC 
CCAAGAGGAG AGCGTGGTGA GAAGGGAGAG TCCGGGCAGC CTGGCACCCC TGGTCCTCCT 
GGAGGAAAGG GACCCACTGG AGATGACGGA CCCAAAGGAA ACCCTGGTCC TGTTGGATTC 
CCTGGTGATC CTGGACCCCC TGGTGAAGTT GGACCACGAG GTCAAGATGG TGCAAAGGGT 
GACAGAGGAG AGGATGGAGA ACAAGGAGAA GCCGGCTCAC CTGGACCTAC TGGAGAGAAC 
GGACCACCTG GACCTCCAGG AAAGAGAGGT CCTGCAGGCA CAAGGGGACC GGAGGGCCGT 
CAAGGAGAGA AAGGAAGCAA GGGTGATTCA GGTGCTCTTG GTCCTCCCGG AAAAACTGGC 
CCTGTGGGGC CACAGGGACA ACCCGGAAAA CCAGGAACTG AGGGTCTAAG AGGGTTGCCT 
GGAACAGTGG GTGAGCAAGG TTCTCCAGGT GCTGCAGGCC CTAAAGGACC TCCTGGACCT 
CTGGGTCCTC CAGGTCTGGC TGGTCTTCGC GGTGATCCCG GTGCTAAAGG TGAGAAGGGA 
CATCCAGGTA TGCTCGGACT GATCGGGCCT GCTGGAGAAC AAGGAGAGAA GGGAGACAGA 
GGCATGCCAG GACCACAGGG ATCAACAGGA CCCAAGGGAG AAACGGGTAT ATCTGGAGGA 
ACTGGTCCAC TTGGTCCTGC AGGTCCTGCT GGTATGCCTG GCCCGAGGGG TGTCAAAGGA 
GCTAAAGGTG CCAGTGGTGG GGCTGGTCCT AAAGGAGAAA AGGGTGTACA AGGACCACCA 
GGACCACCTG GTCCCCCTGG TGATGTCATC CAGCCGCTGC CCATTCAGAT CCCCAAGAAG 
TCCAAGCGCT CCATCGACGC CAGTAAGATT CTGTCTGAGG CTGACGCTGA GGCTGCCGAC 
ACCACTGGCT CTGAGTTCCT GACTGGTAAT GAAGGAATGG AGGAGATCTT CGGCTCACTT 
AATTCCCTGC GCCAGGAGAT CGAGAGCATG CGCTTCCCAT TGGGCACAAA GGAGAGCCCC 
GGCCGCACCT GCCAGGATCT CCATCTCAGC CAACCAGATC TGAAAGACGG CGAGTATTGG 
ATTGATCCAA ATCAAGGCTG TGCTCGCGAC TCTTTCAAGG TTTACTGTAA CTTCACTGCC 
GGCGGAGAGA CCTGCCTGTA TCCCAGCAAA GCAGTGGAGA ATGTAAAGAT GAACTCTTGG 
ATAAAGGAGA AGCCTGGGTC CTTGTTCAGT CAGTTTGCAA AGGGCAACAA GTTTTCATAT 
GTAGACTCGG TTGGAGAGGC TGTGGGTGTT GTTCAGCTGG GCTTCTTGCG GCTGCTCAGT 
GTCCAGGCCA GACAGAACCT CACCTACCAC TGCCAACGCT CGGTGGCCTG GACTGACCAA 
ACCACCGACG GCGGATACAA GCGGGCGCTG CGTCTGCAGG GGGCCAATGA TGAGGAGATC 
AGCTATGAGA CTAGCCCATA TATCAAAGCC CTCATCGACG GCTGCTCCTA TCGTAAGGGT 
CTGGACAGGA CGGTGCTGGA GGTGAACACA CCTCAGGTGG AGCAGCTCCC TCTGCTGGAC 
ATCAGGATCT CAGACTTTGG GGAGGACAAT CAGAAATTTG GTTTTGAAGT AGGACCTGTC 
TGTTTCCTGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159448.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159505.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.

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CloneID	ODa23893
Clone ID Related Accession (Same CDS sequence)	XM_005159448.4 , XM_005159448.3
Accession Version	XM_005159448.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(XI) chain isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159448.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGACGTAGAT CCTGCTTACT CAGAGGCTGA CCACACCCCC 
ACAGAGACCG ATTATTATTA CACAGTAGAA GAAGAGGCGG CTCCACAGCC AGAGAGTGAT 
CTGACTGGAT ATGACGAAAG CATTAACCAG GTCGACGAGA CTGTCCCCGT TGAGGAAATG 
GATGCTACCA AGACAGGTGG TGAGGTGGCG TCTCAAACGC CAGAAGAGCC CTTCACTGAG 
GAGTACATTA CAAGCGACTT TGGGATGAAG GAGTATGACT ATTCCTACAA GGACTACAAT 
GAGCCCATCA TTGAGAGCAA GACGGAGGAC ACTCTAGGCC CTGCCCTGTC CGCTGTGACA 
GAGGAGGGGG GTGCCTCAGT GCGAGCCATT AAGGGAGAGA AGGGAGAGCC TGCTGTCCTT 
GAACCTGGCA TGCTGATTGA AGGACCACCA GGACCAGAAG GACCAGCAGG TCTCCCTGGC 
CCCTCTGGCC CCCAAGGACA ACCTGGTTCT GTTGGTGATC CTGGTGAGAG GGGTCCTACT 
GGTAAGGCTG GTCTACCTGG AGCAGATGGT GTTCCAGGAC CTCCAGGAAC CTCTGTCATG 
CTTCCTTTCC GCTTTGGACA GAGCGCTGGA GAGAAAGGAC CTGTTGCCTC TGCACAGGAA 
GCCCAAGCTC AAGCCATTCT GTCTCAAGCT CGGCTTGCCC TGAAAGGACC ATCCGGGCCA 
ATGGGTTTCA CTGGCCGTCC TGGACCTTTG GGAACACCTG GAAGTCCTGG CTTTAAGGGA 
GAAAGGGGGG ACCCTGGTGC TCAGGGTCCT AGAGGTCCTC AGGGTGTGTC TGGTCCTCCT 
GGCAAAGCTG GTCGGAGGGG TCGCGCTGGT GCTGATGGAG CCAGAGGGAT GCCAGGAGAG 
TCTGGAATTA AGGGTGACCG TGGTTTTGAT GGCCTTCCTG GTTTGCCTGG TGACAAGGGT 
TATCGGGGTC AACCTGGAGG AATGGGACCC CCAGGACCAC ATGGAGAGGA CGGAGAGAGG 
GGAGATGATG GAGATGTTGG ACCCAGAGGT CTTCCTGGCG AACCTGGACC CCGTGGTTTG 
TTGGGGCCTA AAGGGCCTTC TGGACTTCCT GGACCTCCTG GTGTTCGTGG AAATGATGGT 
CCACATGGTC CAAAAGGAAA CTTAGGACCA CAAGGAGAGC CCGGTCCTCC AGGCCAGCAG 
GGGGCTCCAG GAACACAGGG AATGCCAGGT CCACAGGGTG CCACTGGACC ACCAGGAGAG 
AAAGGACCCA CAGGAAAGCC AGGTCTACCA GGAATGCCAG GAGCTGATGG TCCTCCCGGT 
CATCCCGGGA AGGAAGGTCC TGGTGGAACC AAAGGAAATC AAGGTCCTAA TGGTCCTCAG 
GGCTCTATTG GCTATCCTGG CCCTCGGGGA TTGAAGGGAG CTCAAGGTAT CCGTGGACTG 
AAAGGTCATA AAGGAGAGAA GGGAGAGGAT GGATTCCCAG GAATTAAAGG AGACTTTGGT 
GTGAAGGGAG AGAGGGGAGA GGTGGGCGTG CCCGGCCCAA GAGGAGAAGA TGGTCCTGAG 
GGGCCAAAGG GTCGCGTCGG ACCCCCTGGT GAAATTGGTC CCCTCGGCCT GTTGGGAGAG 
AAGGGTAAAC TTGGTGTTCC TGGACTTCCT GGATATCCTG GACGTCAAGG AATAAAGGGG 
TCCCTTGGTT TCCCAGGATT CCCTGGTACA AATGGCGAGA AGGGCACAAG GGGTTTGATT 
GGAAAACCTG GACCAAGAGG ACAAAGAGGG CCGACAGGTC CTAGAGGGCA GAGAGGACCA 
CGTGGGGCAA CTGGGAAATC AGGTGCTAAG GGTGGATCAG GAAGTGACGG GCCTCCTGGA 
CCACCAGGAG AGAGAGGGCT AACTGGACCT CAGGGTGCTA ATGGATTCCC TGGGCCAAAG 
GGACCTCCCG GACCTCCAGG GAAAGATGGA CTTCCTGGAC ACCCTGGACA GAGAGGCGAA 
GTCGGTTTCC AAGGCAAAGT TGGACCACCT GGCCCACCTG GAGTTGTTGG CCCACACGGT 
CCCTCTGGTG AGACTGGTCA GATGGGTGAG CGTGGCCACC CTGGACCTCC AGGACCACCT 
GGTGAGCAAG GCCTTTCTGG CTCCTCTGGT AAAGAAGGAA CCAAAGGAGA TCCCGGTCCT 
CCTGGTGGCC CCGGTAAAGA TGGACCCCCT GGACTTAGAG GATTCCCAGG AGAGAGAGGA 
CTACCAGGAA CTCCAGGTTC TGGAGGACTG AAAGGAAATG AAGGCCCTGC TGGACCACCT 
GGTCCTGCGG GTTCATCTGG GGAGAGAGGT GGTGCTGGCA CTGCAGGCCC TGTCGGCCCA 
CCTGGACGAC CTGGACCTCA GGGACCCCCT GGAACTTCAG GTGAAAAGGG AGTACCAGGC 
GAGAAAGGTC CGGTTGGTCC AGCTGGTCGT GATGGCATCC AAGGCCCAGT TGGTCTCCCA 
GGCCCTGCCG GACCTCCAGG TATTTCTGGA GAAGATGGAG ACAAGGGTGA GGTTGGAGAG 
CCAGGACAAA AGGGAGCTAA AGGGTCCAAA GGAGAACATG GTCCTCCTGG TCCACCTGGG 
CCGATGGGTC CTGTTGGACA GCCTGGTGCA GCGGGTGCTG ATGGTGAGAC GGGTCCAAGA 
GGTCAACAGG GACAGTTTGG TGCCAAGGGA GATGAAGGCA CAAGAGGTTT CCCTGGTCCC 
CCTGGCCCCA TAGGATTGCA GGGTTTGCCA GGCCCAGGTG GAGAAAAGGG AGAGACAGGA 
GATGTTGGAC CCTTGGGTCC TCCTGGTCCT CCAGGGCCGC GTGGTCCTGC TGGTCCTAAT 
GGTGCTGATG GTCCCCAAGG TCCTCCAGGA GGTTTGGGTA ATCCAGGTCC CATAGGAGAG 
AAGGGTGAGC CTGGTGAATC TGGACCACCT GGTGTTGGTG GAGAGCCAGG AAAATTGGGC 
CCAAGAGGAG AGCGTGGTGA GAAGGGAGAG TCCGGGCAGC CTGGCACCCC TGGTCCTCCT 
GGAGGAAAGG GACCCACTGG AGATGACGGA CCCAAAGGAA ACCCTGGTCC TGTTGGATTC 
CCTGGTGATC CTGGACCCCC TGGTGAAGTT GGACCACGAG GTCAAGATGG TGCAAAGGGT 
GACAGAGGAG AGGATGGAGA ACAAGGAGAA GCCGGCTCAC CTGGACCTAC TGGAGAGAAC 
GGACCACCTG GACCTCCAGG AAAGAGAGGT CCTGCAGGCA CAAGGGGACC GGAGGGCCGT 
CAAGGAGAGA AAGGAAGCAA GGGTGATTCA GGTGCTCTTG GTCCTCCCGG AAAAACTGGC 
CCTGTGGGGC CACAGGGACA ACCCGGAAAA CCAGGAACTG AGGGTCTAAG AGGGTTGCCT 
GGAACAGTGG GTGAGCAAGG TTCTCCAGGT GCTGCAGGCC CTAAAGGACC TCCTGGACCT 
CTGGGTCCTC CAGGTCTGGC TGGTCTTCGC GGTGATCCCG GTGCTAAAGG TGAGAAGGGA 
CATCCAGGTA TGCTCGGACT GATCGGGCCT GCTGGAGAAC AAGGAGAGAA GGGAGACAGA 
GGCATGCCAG GACCACAGGG ATCAACAGGA CCCAAGGGAG AAACGGGTAT ATCTGGAGGA 
ACTGGTCCAC TTGGTCCTGC AGGTCCTGCT GGTATGCCTG GCCCGAGGGG TGTCAAAGGA 
GCTAAAGGTG CCAGTGGTGG GGCTGGTCCT AAAGGAGAAA AGGGTGTACA AGGACCACCA 
GGACCACCTG GTCCCCCTGG TGATGTCATC CAGCCGCTGC CCATTCAGAT CCCCAAGAAG 
TCCAAGCGCT CCATCGACGC CAGTAAGATT CTGTCTGAGG CTGACGCTGA GGCTGCCGAC 
ACCACTGGCT CTGAGTTCCT GACTGGTAAT GAAGGAATGG AGGAGATCTT CGGCTCACTT 
AATTCCCTGC GCCAGGAGAT CGAGAGCATG CGCTTCCCAT TGGGCACAAA GGAGAGCCCC 
GGCCGCACCT GCCAGGATCT CCATCTCAGC CAACCAGATC TGAAAGACGG CGAGTATTGG 
ATTGATCCAA ATCAAGGCTG TGCTCGCGAC TCTTTCAAGG TTTACTGTAA CTTCACTGCC 
GGCGGAGAGA CCTGCCTGTA TCCCAGCAAA GCAGTGGAGA ATGTAAAGAT GAACTCTTGG 
ATAAAGGAGA AGCCTGGGTC CTTGTTCAGT CAGTTTGCAA AGGGCAACAA GTTTTCATAT 
GTAGACTCGG TTGGAGAGGC TGTGGGTGTT GTTCAGCTGG GCTTCTTGCG GCTGCTCAGT 
GTCCAGGCCA GACAGAACCT CACCTACCAC TGCCAACGCT CGGTGGCCTG GACTGACCAA 
ACCACCGACG GCGGATACAA GCGGGCGCTG CGTCTGCAGG GGGCCAATGA TGAGGAGATC 
AGCTATGAGA CTAGCCCATA TATCAAAGCC CTCATCGACG GCTGCTCCTA TCGTAAGGGT 
CTGGACAGGA CGGTGCTGGA GGTGAACACA CCTCAGGTGG AGCAGCTCCC TCTGCTGGAC 
ATCAGGATCT CAGACTTTGG GGAGGACAAT CAGAAATTTG GTTTTGAAGT AGGACCTGTC 
TGTTTCCTGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159505.1
CDS	384..5678
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159448.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159448.3

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGACGTAGAT CCTGCTTACT CAGAGGCTGA CCACACCCCC 
ACAGAGACCG ATTATTATTA CACAGTAGAA GAAGAGGCGG CTCCACAGCC AGAGAGTGAT 
CTGACTGGAT ATGACGAAAG CATTAACCAG GTCGACGAGA CTGTCCCCGT TGAGGAAATG 
GATGCTACCA AGACAGGTGG TGAGGTGGCG TCTCAAACGC CAGAAGAGCC CTTCACTGAG 
GAGTACATTA CAAGCGACTT TGGGATGAAG GAGTATGACT ATTCCTACAA GGACTACAAT 
GAGCCCATCA TTGAGAGCAA GACGGAGGAC ACTCTAGGCC CTGCCCTGTC CGCTGTGACA 
GAGGAGGGGG GTGCCTCAGT GCGAGCCATT AAGGGAGAGA AGGGAGAGCC TGCTGTCCTT 
GAACCTGGCA TGCTGATTGA AGGACCACCA GGACCAGAAG GACCAGCAGG TCTCCCTGGC 
CCCTCTGGCC CCCAAGGACA ACCTGGTTCT GTTGGTGATC CTGGTGAGAG GGGTCCTACT 
GGTAAGGCTG GTCTACCTGG AGCAGATGGT GTTCCAGGAC CTCCAGGAAC CTCTGTCATG 
CTTCCTTTCC GCTTTGGACA GAGCGCTGGA GAGAAAGGAC CTGTTGCCTC TGCACAGGAA 
GCCCAAGCTC AAGCCATTCT GTCTCAAGCT CGGCTTGCCC TGAAAGGACC ATCCGGGCCA 
ATGGGTTTCA CTGGCCGTCC TGGACCTTTG GGAACACCTG GAAGTCCTGG CTTTAAGGGA 
GAAAGGGGGG ACCCTGGTGC TCAGGGTCCT AGAGGTCCTC AGGGTGTGTC TGGTCCTCCT 
GGCAAAGCTG GTCGGAGGGG TCGCGCTGGT GCTGATGGAG CCAGAGGGAT GCCAGGAGAG 
TCTGGAATTA AGGGTGACCG TGGTTTTGAT GGCCTTCCTG GTTTGCCTGG TGACAAGGGT 
TATCGGGGTC AACCTGGAGG AATGGGACCC CCAGGACCAC ATGGAGAGGA CGGAGAGAGG 
GGAGATGATG GAGATGTTGG ACCCAGAGGT CTTCCTGGCG AACCTGGACC CCGTGGTTTG 
TTGGGGCCTA AAGGGCCTTC TGGACTTCCT GGACCTCCTG GTGTTCGTGG AAATGATGGT 
CCACATGGTC CAAAAGGAAA CTTAGGACCA CAAGGAGAGC CCGGTCCTCC AGGCCAGCAG 
GGGGCTCCAG GAACACAGGG AATGCCAGGT CCACAGGGTG CCACTGGACC ACCAGGAGAG 
AAAGGACCCA CAGGAAAGCC AGGTCTACCA GGAATGCCAG GAGCTGATGG TCCTCCCGGT 
CATCCCGGGA AGGAAGGTCC TGGTGGAACC AAAGGAAATC AAGGTCCTAA TGGTCCTCAG 
GGCTCTATTG GCTATCCTGG CCCTCGGGGA TTGAAGGGAG CTCAAGGTAT CCGTGGACTG 
AAAGGTCATA AAGGAGAGAA GGGAGAGGAT GGATTCCCAG GAATTAAAGG AGACTTTGGT 
GTGAAGGGAG AGAGGGGAGA GGTGGGCGTG CCCGGCCCAA GAGGAGAAGA TGGTCCTGAG 
GGGCCAAAGG GTCGCGTCGG ACCCCCTGGT GAAATTGGTC CCCTCGGCCT GTTGGGAGAG 
AAGGGTAAAC TTGGTGTTCC TGGACTTCCT GGATATCCTG GACGTCAAGG AATAAAGGGG 
TCCCTTGGTT TCCCAGGATT CCCTGGTACA AATGGCGAGA AGGGCACAAG GGGTTTGATT 
GGAAAACCTG GACCAAGAGG ACAAAGAGGG CCGACAGGTC CTAGAGGGCA GAGAGGACCA 
CGTGGGGCAA CTGGGAAATC AGGTGCTAAG GGTGGATCAG GAAGTGACGG GCCTCCTGGA 
CCACCAGGAG AGAGAGGGCT AACTGGACCT CAGGGTGCTA ATGGATTCCC TGGGCCAAAG 
GGACCTCCCG GACCTCCAGG GAAAGATGGA CTTCCTGGAC ACCCTGGACA GAGAGGCGAA 
GTCGGTTTCC AAGGCAAAGT TGGACCACCT GGCCCACCTG GAGTTGTTGG CCCACACGGT 
CCCTCTGGTG AGACTGGTCA GATGGGTGAG CGTGGCCACC CTGGACCTCC AGGACCACCT 
GGTGAGCAAG GCCTTTCTGG CTCCTCTGGT AAAGAAGGAA CCAAAGGAGA TCCCGGTCCT 
CCTGGTGGCC CCGGTAAAGA TGGACCCCCT GGACTTAGAG GATTCCCAGG AGAGAGAGGA 
CTACCAGGAA CTCCAGGTTC TGGAGGACTG AAAGGAAATG AAGGCCCTGC TGGACCACCT 
GGTCCTGCGG GTTCATCTGG GGAGAGAGGT GGTGCTGGCA CTGCAGGCCC TGTCGGCCCA 
CCTGGACGAC CTGGACCTCA GGGACCCCCT GGAACTTCAG GTGAAAAGGG AGTACCAGGC 
GAGAAAGGTC CGGTTGGTCC AGCTGGTCGT GATGGCATCC AAGGCCCAGT TGGTCTCCCA 
GGCCCTGCCG GACCTCCAGG TATTTCTGGA GAAGATGGAG ACAAGGGTGA GGTTGGAGAG 
CCAGGACAAA AGGGAGCTAA AGGGTCCAAA GGAGAACATG GTCCTCCTGG TCCACCTGGG 
CCGATGGGTC CTGTTGGACA GCCTGGTGCA GCGGGTGCTG ATGGTGAGAC GGGTCCAAGA 
GGTCAACAGG GACAGTTTGG TGCCAAGGGA GATGAAGGCA CAAGAGGTTT CCCTGGTCCC 
CCTGGCCCCA TAGGATTGCA GGGTTTGCCA GGCCCAGGTG GAGAAAAGGG AGAGACAGGA 
GATGTTGGAC CCTTGGGTCC TCCTGGTCCT CCAGGGCCGC GTGGTCCTGC TGGTCCTAAT 
GGTGCTGATG GTCCCCAAGG TCCTCCAGGA GGTTTGGGTA ATCCAGGTCC CATAGGAGAG 
AAGGGTGAGC CTGGTGAATC TGGACCACCT GGTGTTGGTG GAGAGCCAGG AAAATTGGGC 
CCAAGAGGAG AGCGTGGTGA GAAGGGAGAG TCCGGGCAGC CTGGCACCCC TGGTCCTCCT 
GGAGGAAAGG GACCCACTGG AGATGACGGA CCCAAAGGAA ACCCTGGTCC TGTTGGATTC 
CCTGGTGATC CTGGACCCCC TGGTGAAGTT GGACCACGAG GTCAAGATGG TGCAAAGGGT 
GACAGAGGAG AGGATGGAGA ACAAGGAGAA GCCGGCTCAC CTGGACCTAC TGGAGAGAAC 
GGACCACCTG GACCTCCAGG AAAGAGAGGT CCTGCAGGCA CAAGGGGACC GGAGGGCCGT 
CAAGGAGAGA AAGGAAGCAA GGGTGATTCA GGTGCTCTTG GTCCTCCCGG AAAAACTGGC 
CCTGTGGGGC CACAGGGACA ACCCGGAAAA CCAGGAACTG AGGGTCTAAG AGGGTTGCCT 
GGAACAGTGG GTGAGCAAGG TTCTCCAGGT GCTGCAGGCC CTAAAGGACC TCCTGGACCT 
CTGGGTCCTC CAGGTCTGGC TGGTCTTCGC GGTGATCCCG GTGCTAAAGG TGAGAAGGGA 
CATCCAGGTA TGCTCGGACT GATCGGGCCT GCTGGAGAAC AAGGAGAGAA GGGAGACAGA 
GGCATGCCAG GACCACAGGG ATCAACAGGA CCCAAGGGAG AAACGGGTAT ATCTGGAGGA 
ACTGGTCCAC TTGGTCCTGC AGGTCCTGCT GGTATGCCTG GCCCGAGGGG TGTCAAAGGA 
GCTAAAGGTG CCAGTGGTGG GGCTGGTCCT AAAGGAGAAA AGGGTGTACA AGGACCACCA 
GGACCACCTG GTCCCCCTGG TGATGTCATC CAGCCGCTGC CCATTCAGAT CCCCAAGAAG 
TCCAAGCGCT CCATCGACGC CAGTAAGATT CTGTCTGAGG CTGACGCTGA GGCTGCCGAC 
ACCACTGGCT CTGAGTTCCT GACTGGTAAT GAAGGAATGG AGGAGATCTT CGGCTCACTT 
AATTCCCTGC GCCAGGAGAT CGAGAGCATG CGCTTCCCAT TGGGCACAAA GGAGAGCCCC 
GGCCGCACCT GCCAGGATCT CCATCTCAGC CAACCAGATC TGAAAGACGG CGAGTATTGG 
ATTGATCCAA ATCAAGGCTG TGCTCGCGAC TCTTTCAAGG TTTACTGTAA CTTCACTGCC 
GGCGGAGAGA CCTGCCTGTA TCCCAGCAAA GCAGTGGAGA ATGTAAAGAT GAACTCTTGG 
ATAAAGGAGA AGCCTGGGTC CTTGTTCAGT CAGTTTGCAA AGGGCAACAA GTTTTCATAT 
GTAGACTCGG TTGGAGAGGC TGTGGGTGTT GTTCAGCTGG GCTTCTTGCG GCTGCTCAGT 
GTCCAGGCCA GACAGAACCT CACCTACCAC TGCCAACGCT CGGTGGCCTG GACTGACCAA 
ACCACCGACG GCGGATACAA GCGGGCGCTG CGTCTGCAGG GGGCCAATGA TGAGGAGATC 
AGCTATGAGA CTAGCCCATA TATCAAAGCC CTCATCGACG GCTGCTCCTA TCGTAAGGGT 
CTGGACAGGA CGGTGCTGGA GGTGAACACA CCTCAGGTGG AGCAGCTCCC TCTGCTGGAC 
ATCAGGATCT CAGACTTTGG GGAGGACAAT CAGAAATTTG GTTTTGAAGT AGGACCTGTC 
TGTTTCCTGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159450.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159507.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.

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CloneID	ODa23895
Clone ID Related Accession (Same CDS sequence)	XM_005159450.3 , XM_005159450.4
Accession Version	XM_005159450.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(XI) chain isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159507.1
CDS	384..5549
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159450.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159450.3

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159450.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159507.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X7, mRNA.

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CloneID	ODa23895
Clone ID Related Accession (Same CDS sequence)	XM_005159450.3 , XM_005159450.4
Accession Version	XM_005159450.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159450.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159507.1
CDS	392..5557
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159450.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159450.4

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_009294049.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292324.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X8, mRNA.

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CloneID	ODa43572
Clone ID Related Accession (Same CDS sequence)	XM_009294049.2 , NM_001079992.2 , XM_009294049.3
Accession Version	XM_009294049.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(XI) chain isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCGCAGGTCA AAGCACAACC AACCAAAAGC AAGACAGACA 
AAACGAAAGC CAATGCGTCC CCCGCAAAAC CAAAAGACAC CAAAGCCAAA GCAGCCTCCG 
TCACTCAAAA ACCTGCTAAA TTTCCCACCC TTCGACAAGG CTGTTGCCTC AGTGCGAGCC 
ATTAAGGGAG AGAAGGGAGA GCCTGCTGTC CTTGAACCTG GCATGCTGAT TGAAGGACCA 
CCAGGACCAG AAGGACCAGC AGGTCTCCCT GGCCCCTCTG GCCCCCAAGG ACAACCTGGT 
TCTGTTGGTG ATCCTGGTGA GAGGGGTCCT ACTGGTAAGG CTGGTCTACC TGGAGCAGAT 
GGTGTTCCAG GACCTCCAGG AACCTCTGTC ATGCTTCCTT TCCGCTTTGG ACAGAGCGCT 
GGAGAGAAAG GACCTGTTGC CTCTGCACAG GAAGCCCAAG CTCAAGCCAT TCTGTCTCAA 
GCTCGGCTTG CCCTGAAAGG ACCATCCGGG CCAATGGGTT TCACTGGCCG TCCTGGACCT 
TTGGGAACAC CTGGAAGTCC TGGCTTTAAG GGAGAAAGGG GGGACCCTGG TGCTCAGGGT 
CCTAGAGGTC CTCAGGGTGT GTCTGGTCCT CCTGGCAAAG CTGGTCGGAG GGGTCGCGCT 
GGTGCTGATG GAGCCAGAGG GATGCCAGGA GAGTCTGGAA TTAAGGGTGA CCGTGGTTTT 
GATGGCCTTC CTGGTTTGCC TGGTGACAAG GGTTATCGGG GTCAACCTGG AGGAATGGGA 
CCCCCAGGAC CACATGGAGA GGACGGAGAG AGGGGAGATG ATGGAGATGT TGGACCCAGA 
GGTCTTCCTG GCGAACCTGG ACCCCGTGGT TTGTTGGGGC CTAAAGGGCC TTCTGGACTT 
CCTGGACCTC CTGGTGTTCG TGGAAATGAT GGTCCACATG GTCCAAAAGG AAACTTAGGA 
CCACAAGGAG AGCCCGGTCC TCCAGGCCAG CAGGGGGCTC CAGGAACACA GGGAATGCCA 
GGTCCACAGG GTGCCACTGG ACCACCAGGA GAGAAAGGAC CCACAGGAAA GCCAGGTCTA 
CCAGGAATGC CAGGAGCTGA TGGTCCTCCC GGTCATCCCG GGAAGGAAGG TCCTGGTGGA 
ACCAAAGGAA ATCAAGGTCC TAATGGTCCT CAGGGCTCTA TTGGCTATCC TGGCCCTCGG 
GGATTGAAGG GAGCTCAAGG TATCCGTGGA CTGAAAGGTC ATAAAGGAGA GAAGGGAGAG 
GATGGATTCC CAGGAATTAA AGGAGACTTT GGTGTGAAGG GAGAGAGGGG AGAGGTGGGC 
GTGCCCGGCC CAAGAGGAGA AGATGGTCCT GAGGGGCCAA AGGGTCGCGT CGGACCCCCT 
GGTGAAATTG GTCCCCTCGG CCTGTTGGGA GAGAAGGGTA AACTTGGTGT TCCTGGACTT 
CCTGGATATC CTGGACGTCA AGGAATAAAG GGGTCCCTTG GTTTCCCAGG ATTCCCTGGT 
ACAAATGGCG AGAAGGGCAC AAGGGGTTTG ATTGGAAAAC CTGGACCAAG AGGACAAAGA 
GGGCCGACAG GTCCTAGAGG GCAGAGAGGA CCACGTGGGG CAACTGGGAA ATCAGGTGCT 
AAGGGTGGAT CAGGAAGTGA CGGGCCTCCT GGACCACCAG GAGAGAGAGG GCTAACTGGA 
CCTCAGGGTG CTAATGGATT CCCTGGGCCA AAGGGACCTC CCGGACCTCC AGGGAAAGAT 
GGACTTCCTG GACACCCTGG ACAGAGAGGC GAAGTCGGTT TCCAAGGCAA AGTTGGACCA 
CCTGGCCCAC CTGGAGTTGT TGGCCCACAC GGTCCCTCTG GTGAGACTGG TCAGATGGGT 
GAGCGTGGCC ACCCTGGACC TCCAGGACCA CCTGGTGAGC AAGGCCTTTC TGGCTCCTCT 
GGTAAAGAAG GAACCAAAGG AGATCCCGGT CCTCCTGGTG GCCCCGGTAA AGATGGACCC 
CCTGGACTTA GAGGATTCCC AGGAGAGAGA GGACTACCAG GAACTCCAGG TTCTGGAGGA 
CTGAAAGGAA ATGAAGGCCC TGCTGGACCA CCTGGTCCTG CGGGTTCATC TGGGGAGAGA 
GGTGGTGCTG GCACTGCAGG CCCTGTCGGC CCACCTGGAC GACCTGGACC TCAGGGACCC 
CCTGGAACTT CAGGTGAAAA GGGAGTACCA GGCGAGAAAG GTCCGGTTGG TCCAGCTGGT 
CGTGATGGCA TCCAAGGCCC AGTTGGTCTC CCAGGCCCTG CCGGACCTCC AGGTATTTCT 
GGAGAAGATG GAGACAAGGG TGAGGTTGGA GAGCCAGGAC AAAAGGGAGC TAAAGGGTCC 
AAAGGAGAAC ATGGTCCTCC TGGTCCACCT GGGCCGATGG GTCCTGTTGG ACAGCCTGGT 
GCAGCGGGTG CTGATGGTGA GACGGGTCCA AGAGGTCAAC AGGGACAGTT TGGTGCCAAG 
GGAGATGAAG GCACAAGAGG TTTCCCTGGT CCCCCTGGCC CCATAGGATT GCAGGGTTTG 
CCAGGCCCAG GTGGAGAAAA GGGAGAGACA GGAGATGTTG GACCCTTGGG TCCTCCTGGT 
CCTCCAGGGC CGCGTGGTCC TGCTGGTCCT AATGGTGCTG ATGGTCCCCA AGGTCCTCCA 
GGAGGTTTGG GTAATCCAGG TCCCATAGGA GAGAAGGGTG AGCCTGGTGA ATCTGGACCA 
CCTGGTGTTG GTGGAGAGCC AGGAAAATTG GGCCCAAGAG GAGAGCGTGG TGAGAAGGGA 
GAGTCCGGGC AGCCTGGCAC CCCTGGTCCT CCTGGAGGAA AGGGACCCAC TGGAGATGAC 
GGACCCAAAG GAAACCCTGG TCCTGTTGGA TTCCCTGGTG ATCCTGGACC CCCTGGTGAA 
GTTGGACCAC GAGGTCAAGA TGGTGCAAAG GGTGACAGAG GAGAGGATGG AGAACAAGGA 
GAAGCCGGCT CACCTGGACC TACTGGAGAG AACGGACCAC CTGGACCTCC AGGAAAGAGA 
GGTCCTGCAG GCACAAGGGG ACCGGAGGGC CGTCAAGGAG AGAAAGGAAG CAAGGGTGAT 
TCAGGTGCTC TTGGTCCTCC CGGAAAAACT GGCCCTGTGG GGCCACAGGG ACAACCCGGA 
AAACCAGGAA CTGAGGGTCT AAGAGGGTTG CCTGGAACAG TGGGTGAGCA AGGTTCTCCA 
GGTGCTGCAG GCCCTAAAGG ACCTCCTGGA CCTCTGGGTC CTCCAGGTCT GGCTGGTCTT 
CGCGGTGATC CCGGTGCTAA AGGTGAGAAG GGACATCCAG GTATGCTCGG ACTGATCGGG 
CCTGCTGGAG AACAAGGAGA GAAGGGAGAC AGAGGCATGC CAGGACCACA GGGATCAACA 
GGACCCAAGG GAGAAACGGG TATATCTGGA GGAACTGGTC CACTTGGTCC TGCAGGTCCT 
GCTGGTATGC CTGGCCCGAG GGGTGTCAAA GGAGCTAAAG GTGCCAGTGG TGGGGCTGGT 
CCTAAAGGAG AAAAGGGTGT ACAAGGACCA CCAGGACCAC CTGGTCCCCC TGGTGATGTC 
ATCCAGCCGC TGCCCATTCA GATCCCCAAG AAGTCCAAGC GCTCCATCGA CGCCAGTAAG 
ATTCTGTCTG AGGCTGACGC TGAGGCTGCC GACACCACTG GCTCTGAGTT CCTGACTGGT 
AATGAAGGAA TGGAGGAGAT CTTCGGCTCA CTTAATTCCC TGCGCCAGGA GATCGAGAGC 
ATGCGCTTCC CATTGGGCAC AAAGGAGAGC CCCGGCCGCA CCTGCCAGGA TCTCCATCTC 
AGCCAACCAG ATCTGAAAGA CGGCGAGTAT TGGATTGATC CAAATCAAGG CTGTGCTCGC 
GACTCTTTCA AGGTTTACTG TAACTTCACT GCCGGCGGAG AGACCTGCCT GTATCCCAGC 
AAAGCAGTGG AGAATGTAAA GATGAACTCT TGGATAAAGG AGAAGCCTGG GTCCTTGTTC 
AGTCAGTTTG CAAAGGGCAA CAAGTTTTCA TATGTAGACT CGGTTGGAGA GGCTGTGGGT 
GTTGTTCAGC TGGGCTTCTT GCGGCTGCTC AGTGTCCAGG CCAGACAGAA CCTCACCTAC 
CACTGCCAAC GCTCGGTGGC CTGGACTGAC CAAACCACCG ACGGCGGATA CAAGCGGGCG 
CTGCGTCTGC AGGGGGCCAA TGATGAGGAG ATCAGCTATG AGACTAGCCC ATATATCAAA 
GCCCTCATCG ACGGCTGCTC CTATCGTAAG GGTCTGGACA GGACGGTGCT GGAGGTGAAC 
ACACCTCAGG TGGAGCAGCT CCCTCTGCTG GACATCAGGA TCTCAGACTT TGGGGAGGAC 
AATCAGAAAT TTGGTTTTGA AGTAGGACCT GTCTGTTTCC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292324.1
CDS	384..5471
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYRRSKHNQPKARQTKRKPMRPPQNQKTPKPKQPPSLKNLLNFPPFDKAVASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_009294049.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294049.2

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCGCAGGTCA AAGCACAACC AACCAAAAGC AAGACAGACA 
AAACGAAAGC CAATGCGTCC CCCGCAAAAC CAAAAGACAC CAAAGCCAAA GCAGCCTCCG 
TCACTCAAAA ACCTGCTAAA TTTCCCACCC TTCGACAAGG CTGTTGCCTC AGTGCGAGCC 
ATTAAGGGAG AGAAGGGAGA GCCTGCTGTC CTTGAACCTG GCATGCTGAT TGAAGGACCA 
CCAGGACCAG AAGGACCAGC AGGTCTCCCT GGCCCCTCTG GCCCCCAAGG ACAACCTGGT 
TCTGTTGGTG ATCCTGGTGA GAGGGGTCCT ACTGGTAAGG CTGGTCTACC TGGAGCAGAT 
GGTGTTCCAG GACCTCCAGG AACCTCTGTC ATGCTTCCTT TCCGCTTTGG ACAGAGCGCT 
GGAGAGAAAG GACCTGTTGC CTCTGCACAG GAAGCCCAAG CTCAAGCCAT TCTGTCTCAA 
GCTCGGCTTG CCCTGAAAGG ACCATCCGGG CCAATGGGTT TCACTGGCCG TCCTGGACCT 
TTGGGAACAC CTGGAAGTCC TGGCTTTAAG GGAGAAAGGG GGGACCCTGG TGCTCAGGGT 
CCTAGAGGTC CTCAGGGTGT GTCTGGTCCT CCTGGCAAAG CTGGTCGGAG GGGTCGCGCT 
GGTGCTGATG GAGCCAGAGG GATGCCAGGA GAGTCTGGAA TTAAGGGTGA CCGTGGTTTT 
GATGGCCTTC CTGGTTTGCC TGGTGACAAG GGTTATCGGG GTCAACCTGG AGGAATGGGA 
CCCCCAGGAC CACATGGAGA GGACGGAGAG AGGGGAGATG ATGGAGATGT TGGACCCAGA 
GGTCTTCCTG GCGAACCTGG ACCCCGTGGT TTGTTGGGGC CTAAAGGGCC TTCTGGACTT 
CCTGGACCTC CTGGTGTTCG TGGAAATGAT GGTCCACATG GTCCAAAAGG AAACTTAGGA 
CCACAAGGAG AGCCCGGTCC TCCAGGCCAG CAGGGGGCTC CAGGAACACA GGGAATGCCA 
GGTCCACAGG GTGCCACTGG ACCACCAGGA GAGAAAGGAC CCACAGGAAA GCCAGGTCTA 
CCAGGAATGC CAGGAGCTGA TGGTCCTCCC GGTCATCCCG GGAAGGAAGG TCCTGGTGGA 
ACCAAAGGAA ATCAAGGTCC TAATGGTCCT CAGGGCTCTA TTGGCTATCC TGGCCCTCGG 
GGATTGAAGG GAGCTCAAGG TATCCGTGGA CTGAAAGGTC ATAAAGGAGA GAAGGGAGAG 
GATGGATTCC CAGGAATTAA AGGAGACTTT GGTGTGAAGG GAGAGAGGGG AGAGGTGGGC 
GTGCCCGGCC CAAGAGGAGA AGATGGTCCT GAGGGGCCAA AGGGTCGCGT CGGACCCCCT 
GGTGAAATTG GTCCCCTCGG CCTGTTGGGA GAGAAGGGTA AACTTGGTGT TCCTGGACTT 
CCTGGATATC CTGGACGTCA AGGAATAAAG GGGTCCCTTG GTTTCCCAGG ATTCCCTGGT 
ACAAATGGCG AGAAGGGCAC AAGGGGTTTG ATTGGAAAAC CTGGACCAAG AGGACAAAGA 
GGGCCGACAG GTCCTAGAGG GCAGAGAGGA CCACGTGGGG CAACTGGGAA ATCAGGTGCT 
AAGGGTGGAT CAGGAAGTGA CGGGCCTCCT GGACCACCAG GAGAGAGAGG GCTAACTGGA 
CCTCAGGGTG CTAATGGATT CCCTGGGCCA AAGGGACCTC CCGGACCTCC AGGGAAAGAT 
GGACTTCCTG GACACCCTGG ACAGAGAGGC GAAGTCGGTT TCCAAGGCAA AGTTGGACCA 
CCTGGCCCAC CTGGAGTTGT TGGCCCACAC GGTCCCTCTG GTGAGACTGG TCAGATGGGT 
GAGCGTGGCC ACCCTGGACC TCCAGGACCA CCTGGTGAGC AAGGCCTTTC TGGCTCCTCT 
GGTAAAGAAG GAACCAAAGG AGATCCCGGT CCTCCTGGTG GCCCCGGTAA AGATGGACCC 
CCTGGACTTA GAGGATTCCC AGGAGAGAGA GGACTACCAG GAACTCCAGG TTCTGGAGGA 
CTGAAAGGAA ATGAAGGCCC TGCTGGACCA CCTGGTCCTG CGGGTTCATC TGGGGAGAGA 
GGTGGTGCTG GCACTGCAGG CCCTGTCGGC CCACCTGGAC GACCTGGACC TCAGGGACCC 
CCTGGAACTT CAGGTGAAAA GGGAGTACCA GGCGAGAAAG GTCCGGTTGG TCCAGCTGGT 
CGTGATGGCA TCCAAGGCCC AGTTGGTCTC CCAGGCCCTG CCGGACCTCC AGGTATTTCT 
GGAGAAGATG GAGACAAGGG TGAGGTTGGA GAGCCAGGAC AAAAGGGAGC TAAAGGGTCC 
AAAGGAGAAC ATGGTCCTCC TGGTCCACCT GGGCCGATGG GTCCTGTTGG ACAGCCTGGT 
GCAGCGGGTG CTGATGGTGA GACGGGTCCA AGAGGTCAAC AGGGACAGTT TGGTGCCAAG 
GGAGATGAAG GCACAAGAGG TTTCCCTGGT CCCCCTGGCC CCATAGGATT GCAGGGTTTG 
CCAGGCCCAG GTGGAGAAAA GGGAGAGACA GGAGATGTTG GACCCTTGGG TCCTCCTGGT 
CCTCCAGGGC CGCGTGGTCC TGCTGGTCCT AATGGTGCTG ATGGTCCCCA AGGTCCTCCA 
GGAGGTTTGG GTAATCCAGG TCCCATAGGA GAGAAGGGTG AGCCTGGTGA ATCTGGACCA 
CCTGGTGTTG GTGGAGAGCC AGGAAAATTG GGCCCAAGAG GAGAGCGTGG TGAGAAGGGA 
GAGTCCGGGC AGCCTGGCAC CCCTGGTCCT CCTGGAGGAA AGGGACCCAC TGGAGATGAC 
GGACCCAAAG GAAACCCTGG TCCTGTTGGA TTCCCTGGTG ATCCTGGACC CCCTGGTGAA 
GTTGGACCAC GAGGTCAAGA TGGTGCAAAG GGTGACAGAG GAGAGGATGG AGAACAAGGA 
GAAGCCGGCT CACCTGGACC TACTGGAGAG AACGGACCAC CTGGACCTCC AGGAAAGAGA 
GGTCCTGCAG GCACAAGGGG ACCGGAGGGC CGTCAAGGAG AGAAAGGAAG CAAGGGTGAT 
TCAGGTGCTC TTGGTCCTCC CGGAAAAACT GGCCCTGTGG GGCCACAGGG ACAACCCGGA 
AAACCAGGAA CTGAGGGTCT AAGAGGGTTG CCTGGAACAG TGGGTGAGCA AGGTTCTCCA 
GGTGCTGCAG GCCCTAAAGG ACCTCCTGGA CCTCTGGGTC CTCCAGGTCT GGCTGGTCTT 
CGCGGTGATC CCGGTGCTAA AGGTGAGAAG GGACATCCAG GTATGCTCGG ACTGATCGGG 
CCTGCTGGAG AACAAGGAGA GAAGGGAGAC AGAGGCATGC CAGGACCACA GGGATCAACA 
GGACCCAAGG GAGAAACGGG TATATCTGGA GGAACTGGTC CACTTGGTCC TGCAGGTCCT 
GCTGGTATGC CTGGCCCGAG GGGTGTCAAA GGAGCTAAAG GTGCCAGTGG TGGGGCTGGT 
CCTAAAGGAG AAAAGGGTGT ACAAGGACCA CCAGGACCAC CTGGTCCCCC TGGTGATGTC 
ATCCAGCCGC TGCCCATTCA GATCCCCAAG AAGTCCAAGC GCTCCATCGA CGCCAGTAAG 
ATTCTGTCTG AGGCTGACGC TGAGGCTGCC GACACCACTG GCTCTGAGTT CCTGACTGGT 
AATGAAGGAA TGGAGGAGAT CTTCGGCTCA CTTAATTCCC TGCGCCAGGA GATCGAGAGC 
ATGCGCTTCC CATTGGGCAC AAAGGAGAGC CCCGGCCGCA CCTGCCAGGA TCTCCATCTC 
AGCCAACCAG ATCTGAAAGA CGGCGAGTAT TGGATTGATC CAAATCAAGG CTGTGCTCGC 
GACTCTTTCA AGGTTTACTG TAACTTCACT GCCGGCGGAG AGACCTGCCT GTATCCCAGC 
AAAGCAGTGG AGAATGTAAA GATGAACTCT TGGATAAAGG AGAAGCCTGG GTCCTTGTTC 
AGTCAGTTTG CAAAGGGCAA CAAGTTTTCA TATGTAGACT CGGTTGGAGA GGCTGTGGGT 
GTTGTTCAGC TGGGCTTCTT GCGGCTGCTC AGTGTCCAGG CCAGACAGAA CCTCACCTAC 
CACTGCCAAC GCTCGGTGGC CTGGACTGAC CAAACCACCG ACGGCGGATA CAAGCGGGCG 
CTGCGTCTGC AGGGGGCCAA TGATGAGGAG ATCAGCTATG AGACTAGCCC ATATATCAAA 
GCCCTCATCG ACGGCTGCTC CTATCGTAAG GGTCTGGACA GGACGGTGCT GGAGGTGAAC 
ACACCTCAGG TGGAGCAGCT CCCTCTGCTG GACATCAGGA TCTCAGACTT TGGGGAGGAC 
AATCAGAAAT TTGGTTTTGA AGTAGGACCT GTCTGTTTCC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_009294049.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292324.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X8, mRNA.

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CloneID	ODa43572
Clone ID Related Accession (Same CDS sequence)	XM_009294049.2 , NM_001079992.2 , XM_009294049.3
Accession Version	XM_009294049.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCGCAGGTCA AAGCACAACC AACCAAAAGC AAGACAGACA 
AAACGAAAGC CAATGCGTCC CCCGCAAAAC CAAAAGACAC CAAAGCCAAA GCAGCCTCCG 
TCACTCAAAA ACCTGCTAAA TTTCCCACCC TTCGACAAGG CTGTTGCCTC AGTGCGAGCC 
ATTAAGGGAG AGAAGGGAGA GCCTGCTGTC CTTGAACCTG GCATGCTGAT TGAAGGACCA 
CCAGGACCAG AAGGACCAGC AGGTCTCCCT GGCCCCTCTG GCCCCCAAGG ACAACCTGGT 
TCTGTTGGTG ATCCTGGTGA GAGGGGTCCT ACTGGTAAGG CTGGTCTACC TGGAGCAGAT 
GGTGTTCCAG GACCTCCAGG AACCTCTGTC ATGCTTCCTT TCCGCTTTGG ACAGAGCGCT 
GGAGAGAAAG GACCTGTTGC CTCTGCACAG GAAGCCCAAG CTCAAGCCAT TCTGTCTCAA 
GCTCGGCTTG CCCTGAAAGG ACCATCCGGG CCAATGGGTT TCACTGGCCG TCCTGGACCT 
TTGGGAACAC CTGGAAGTCC TGGCTTTAAG GGAGAAAGGG GGGACCCTGG TGCTCAGGGT 
CCTAGAGGTC CTCAGGGTGT GTCTGGTCCT CCTGGCAAAG CTGGTCGGAG GGGTCGCGCT 
GGTGCTGATG GAGCCAGAGG GATGCCAGGA GAGTCTGGAA TTAAGGGTGA CCGTGGTTTT 
GATGGCCTTC CTGGTTTGCC TGGTGACAAG GGTTATCGGG GTCAACCTGG AGGAATGGGA 
CCCCCAGGAC CACATGGAGA GGACGGAGAG AGGGGAGATG ATGGAGATGT TGGACCCAGA 
GGTCTTCCTG GCGAACCTGG ACCCCGTGGT TTGTTGGGGC CTAAAGGGCC TTCTGGACTT 
CCTGGACCTC CTGGTGTTCG TGGAAATGAT GGTCCACATG GTCCAAAAGG AAACTTAGGA 
CCACAAGGAG AGCCCGGTCC TCCAGGCCAG CAGGGGGCTC CAGGAACACA GGGAATGCCA 
GGTCCACAGG GTGCCACTGG ACCACCAGGA GAGAAAGGAC CCACAGGAAA GCCAGGTCTA 
CCAGGAATGC CAGGAGCTGA TGGTCCTCCC GGTCATCCCG GGAAGGAAGG TCCTGGTGGA 
ACCAAAGGAA ATCAAGGTCC TAATGGTCCT CAGGGCTCTA TTGGCTATCC TGGCCCTCGG 
GGATTGAAGG GAGCTCAAGG TATCCGTGGA CTGAAAGGTC ATAAAGGAGA GAAGGGAGAG 
GATGGATTCC CAGGAATTAA AGGAGACTTT GGTGTGAAGG GAGAGAGGGG AGAGGTGGGC 
GTGCCCGGCC CAAGAGGAGA AGATGGTCCT GAGGGGCCAA AGGGTCGCGT CGGACCCCCT 
GGTGAAATTG GTCCCCTCGG CCTGTTGGGA GAGAAGGGTA AACTTGGTGT TCCTGGACTT 
CCTGGATATC CTGGACGTCA AGGAATAAAG GGGTCCCTTG GTTTCCCAGG ATTCCCTGGT 
ACAAATGGCG AGAAGGGCAC AAGGGGTTTG ATTGGAAAAC CTGGACCAAG AGGACAAAGA 
GGGCCGACAG GTCCTAGAGG GCAGAGAGGA CCACGTGGGG CAACTGGGAA ATCAGGTGCT 
AAGGGTGGAT CAGGAAGTGA CGGGCCTCCT GGACCACCAG GAGAGAGAGG GCTAACTGGA 
CCTCAGGGTG CTAATGGATT CCCTGGGCCA AAGGGACCTC CCGGACCTCC AGGGAAAGAT 
GGACTTCCTG GACACCCTGG ACAGAGAGGC GAAGTCGGTT TCCAAGGCAA AGTTGGACCA 
CCTGGCCCAC CTGGAGTTGT TGGCCCACAC GGTCCCTCTG GTGAGACTGG TCAGATGGGT 
GAGCGTGGCC ACCCTGGACC TCCAGGACCA CCTGGTGAGC AAGGCCTTTC TGGCTCCTCT 
GGTAAAGAAG GAACCAAAGG AGATCCCGGT CCTCCTGGTG GCCCCGGTAA AGATGGACCC 
CCTGGACTTA GAGGATTCCC AGGAGAGAGA GGACTACCAG GAACTCCAGG TTCTGGAGGA 
CTGAAAGGAA ATGAAGGCCC TGCTGGACCA CCTGGTCCTG CGGGTTCATC TGGGGAGAGA 
GGTGGTGCTG GCACTGCAGG CCCTGTCGGC CCACCTGGAC GACCTGGACC TCAGGGACCC 
CCTGGAACTT CAGGTGAAAA GGGAGTACCA GGCGAGAAAG GTCCGGTTGG TCCAGCTGGT 
CGTGATGGCA TCCAAGGCCC AGTTGGTCTC CCAGGCCCTG CCGGACCTCC AGGTATTTCT 
GGAGAAGATG GAGACAAGGG TGAGGTTGGA GAGCCAGGAC AAAAGGGAGC TAAAGGGTCC 
AAAGGAGAAC ATGGTCCTCC TGGTCCACCT GGGCCGATGG GTCCTGTTGG ACAGCCTGGT 
GCAGCGGGTG CTGATGGTGA GACGGGTCCA AGAGGTCAAC AGGGACAGTT TGGTGCCAAG 
GGAGATGAAG GCACAAGAGG TTTCCCTGGT CCCCCTGGCC CCATAGGATT GCAGGGTTTG 
CCAGGCCCAG GTGGAGAAAA GGGAGAGACA GGAGATGTTG GACCCTTGGG TCCTCCTGGT 
CCTCCAGGGC CGCGTGGTCC TGCTGGTCCT AATGGTGCTG ATGGTCCCCA AGGTCCTCCA 
GGAGGTTTGG GTAATCCAGG TCCCATAGGA GAGAAGGGTG AGCCTGGTGA ATCTGGACCA 
CCTGGTGTTG GTGGAGAGCC AGGAAAATTG GGCCCAAGAG GAGAGCGTGG TGAGAAGGGA 
GAGTCCGGGC AGCCTGGCAC CCCTGGTCCT CCTGGAGGAA AGGGACCCAC TGGAGATGAC 
GGACCCAAAG GAAACCCTGG TCCTGTTGGA TTCCCTGGTG ATCCTGGACC CCCTGGTGAA 
GTTGGACCAC GAGGTCAAGA TGGTGCAAAG GGTGACAGAG GAGAGGATGG AGAACAAGGA 
GAAGCCGGCT CACCTGGACC TACTGGAGAG AACGGACCAC CTGGACCTCC AGGAAAGAGA 
GGTCCTGCAG GCACAAGGGG ACCGGAGGGC CGTCAAGGAG AGAAAGGAAG CAAGGGTGAT 
TCAGGTGCTC TTGGTCCTCC CGGAAAAACT GGCCCTGTGG GGCCACAGGG ACAACCCGGA 
AAACCAGGAA CTGAGGGTCT AAGAGGGTTG CCTGGAACAG TGGGTGAGCA AGGTTCTCCA 
GGTGCTGCAG GCCCTAAAGG ACCTCCTGGA CCTCTGGGTC CTCCAGGTCT GGCTGGTCTT 
CGCGGTGATC CCGGTGCTAA AGGTGAGAAG GGACATCCAG GTATGCTCGG ACTGATCGGG 
CCTGCTGGAG AACAAGGAGA GAAGGGAGAC AGAGGCATGC CAGGACCACA GGGATCAACA 
GGACCCAAGG GAGAAACGGG TATATCTGGA GGAACTGGTC CACTTGGTCC TGCAGGTCCT 
GCTGGTATGC CTGGCCCGAG GGGTGTCAAA GGAGCTAAAG GTGCCAGTGG TGGGGCTGGT 
CCTAAAGGAG AAAAGGGTGT ACAAGGACCA CCAGGACCAC CTGGTCCCCC TGGTGATGTC 
ATCCAGCCGC TGCCCATTCA GATCCCCAAG AAGTCCAAGC GCTCCATCGA CGCCAGTAAG 
ATTCTGTCTG AGGCTGACGC TGAGGCTGCC GACACCACTG GCTCTGAGTT CCTGACTGGT 
AATGAAGGAA TGGAGGAGAT CTTCGGCTCA CTTAATTCCC TGCGCCAGGA GATCGAGAGC 
ATGCGCTTCC CATTGGGCAC AAAGGAGAGC CCCGGCCGCA CCTGCCAGGA TCTCCATCTC 
AGCCAACCAG ATCTGAAAGA CGGCGAGTAT TGGATTGATC CAAATCAAGG CTGTGCTCGC 
GACTCTTTCA AGGTTTACTG TAACTTCACT GCCGGCGGAG AGACCTGCCT GTATCCCAGC 
AAAGCAGTGG AGAATGTAAA GATGAACTCT TGGATAAAGG AGAAGCCTGG GTCCTTGTTC 
AGTCAGTTTG CAAAGGGCAA CAAGTTTTCA TATGTAGACT CGGTTGGAGA GGCTGTGGGT 
GTTGTTCAGC TGGGCTTCTT GCGGCTGCTC AGTGTCCAGG CCAGACAGAA CCTCACCTAC 
CACTGCCAAC GCTCGGTGGC CTGGACTGAC CAAACCACCG ACGGCGGATA CAAGCGGGCG 
CTGCGTCTGC AGGGGGCCAA TGATGAGGAG ATCAGCTATG AGACTAGCCC ATATATCAAA 
GCCCTCATCG ACGGCTGCTC CTATCGTAAG GGTCTGGACA GGACGGTGCT GGAGGTGAAC 
ACACCTCAGG TGGAGCAGCT CCCTCTGCTG GACATCAGGA TCTCAGACTT TGGGGAGGAC 
AATCAGAAAT TTGGTTTTGA AGTAGGACCT GTCTGTTTCC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292324.1
CDS	392..5479
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYRRSKHNQPKARQTKRKPMRPPQNQKTPKPKQPPSLKNLLNFPPFDKAVASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_009294049.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294049.3

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TCGCAGGTCA AAGCACAACC AACCAAAAGC AAGACAGACA 
AAACGAAAGC CAATGCGTCC CCCGCAAAAC CAAAAGACAC CAAAGCCAAA GCAGCCTCCG 
TCACTCAAAA ACCTGCTAAA TTTCCCACCC TTCGACAAGG CTGTTGCCTC AGTGCGAGCC 
ATTAAGGGAG AGAAGGGAGA GCCTGCTGTC CTTGAACCTG GCATGCTGAT TGAAGGACCA 
CCAGGACCAG AAGGACCAGC AGGTCTCCCT GGCCCCTCTG GCCCCCAAGG ACAACCTGGT 
TCTGTTGGTG ATCCTGGTGA GAGGGGTCCT ACTGGTAAGG CTGGTCTACC TGGAGCAGAT 
GGTGTTCCAG GACCTCCAGG AACCTCTGTC ATGCTTCCTT TCCGCTTTGG ACAGAGCGCT 
GGAGAGAAAG GACCTGTTGC CTCTGCACAG GAAGCCCAAG CTCAAGCCAT TCTGTCTCAA 
GCTCGGCTTG CCCTGAAAGG ACCATCCGGG CCAATGGGTT TCACTGGCCG TCCTGGACCT 
TTGGGAACAC CTGGAAGTCC TGGCTTTAAG GGAGAAAGGG GGGACCCTGG TGCTCAGGGT 
CCTAGAGGTC CTCAGGGTGT GTCTGGTCCT CCTGGCAAAG CTGGTCGGAG GGGTCGCGCT 
GGTGCTGATG GAGCCAGAGG GATGCCAGGA GAGTCTGGAA TTAAGGGTGA CCGTGGTTTT 
GATGGCCTTC CTGGTTTGCC TGGTGACAAG GGTTATCGGG GTCAACCTGG AGGAATGGGA 
CCCCCAGGAC CACATGGAGA GGACGGAGAG AGGGGAGATG ATGGAGATGT TGGACCCAGA 
GGTCTTCCTG GCGAACCTGG ACCCCGTGGT TTGTTGGGGC CTAAAGGGCC TTCTGGACTT 
CCTGGACCTC CTGGTGTTCG TGGAAATGAT GGTCCACATG GTCCAAAAGG AAACTTAGGA 
CCACAAGGAG AGCCCGGTCC TCCAGGCCAG CAGGGGGCTC CAGGAACACA GGGAATGCCA 
GGTCCACAGG GTGCCACTGG ACCACCAGGA GAGAAAGGAC CCACAGGAAA GCCAGGTCTA 
CCAGGAATGC CAGGAGCTGA TGGTCCTCCC GGTCATCCCG GGAAGGAAGG TCCTGGTGGA 
ACCAAAGGAA ATCAAGGTCC TAATGGTCCT CAGGGCTCTA TTGGCTATCC TGGCCCTCGG 
GGATTGAAGG GAGCTCAAGG TATCCGTGGA CTGAAAGGTC ATAAAGGAGA GAAGGGAGAG 
GATGGATTCC CAGGAATTAA AGGAGACTTT GGTGTGAAGG GAGAGAGGGG AGAGGTGGGC 
GTGCCCGGCC CAAGAGGAGA AGATGGTCCT GAGGGGCCAA AGGGTCGCGT CGGACCCCCT 
GGTGAAATTG GTCCCCTCGG CCTGTTGGGA GAGAAGGGTA AACTTGGTGT TCCTGGACTT 
CCTGGATATC CTGGACGTCA AGGAATAAAG GGGTCCCTTG GTTTCCCAGG ATTCCCTGGT 
ACAAATGGCG AGAAGGGCAC AAGGGGTTTG ATTGGAAAAC CTGGACCAAG AGGACAAAGA 
GGGCCGACAG GTCCTAGAGG GCAGAGAGGA CCACGTGGGG CAACTGGGAA ATCAGGTGCT 
AAGGGTGGAT CAGGAAGTGA CGGGCCTCCT GGACCACCAG GAGAGAGAGG GCTAACTGGA 
CCTCAGGGTG CTAATGGATT CCCTGGGCCA AAGGGACCTC CCGGACCTCC AGGGAAAGAT 
GGACTTCCTG GACACCCTGG ACAGAGAGGC GAAGTCGGTT TCCAAGGCAA AGTTGGACCA 
CCTGGCCCAC CTGGAGTTGT TGGCCCACAC GGTCCCTCTG GTGAGACTGG TCAGATGGGT 
GAGCGTGGCC ACCCTGGACC TCCAGGACCA CCTGGTGAGC AAGGCCTTTC TGGCTCCTCT 
GGTAAAGAAG GAACCAAAGG AGATCCCGGT CCTCCTGGTG GCCCCGGTAA AGATGGACCC 
CCTGGACTTA GAGGATTCCC AGGAGAGAGA GGACTACCAG GAACTCCAGG TTCTGGAGGA 
CTGAAAGGAA ATGAAGGCCC TGCTGGACCA CCTGGTCCTG CGGGTTCATC TGGGGAGAGA 
GGTGGTGCTG GCACTGCAGG CCCTGTCGGC CCACCTGGAC GACCTGGACC TCAGGGACCC 
CCTGGAACTT CAGGTGAAAA GGGAGTACCA GGCGAGAAAG GTCCGGTTGG TCCAGCTGGT 
CGTGATGGCA TCCAAGGCCC AGTTGGTCTC CCAGGCCCTG CCGGACCTCC AGGTATTTCT 
GGAGAAGATG GAGACAAGGG TGAGGTTGGA GAGCCAGGAC AAAAGGGAGC TAAAGGGTCC 
AAAGGAGAAC ATGGTCCTCC TGGTCCACCT GGGCCGATGG GTCCTGTTGG ACAGCCTGGT 
GCAGCGGGTG CTGATGGTGA GACGGGTCCA AGAGGTCAAC AGGGACAGTT TGGTGCCAAG 
GGAGATGAAG GCACAAGAGG TTTCCCTGGT CCCCCTGGCC CCATAGGATT GCAGGGTTTG 
CCAGGCCCAG GTGGAGAAAA GGGAGAGACA GGAGATGTTG GACCCTTGGG TCCTCCTGGT 
CCTCCAGGGC CGCGTGGTCC TGCTGGTCCT AATGGTGCTG ATGGTCCCCA AGGTCCTCCA 
GGAGGTTTGG GTAATCCAGG TCCCATAGGA GAGAAGGGTG AGCCTGGTGA ATCTGGACCA 
CCTGGTGTTG GTGGAGAGCC AGGAAAATTG GGCCCAAGAG GAGAGCGTGG TGAGAAGGGA 
GAGTCCGGGC AGCCTGGCAC CCCTGGTCCT CCTGGAGGAA AGGGACCCAC TGGAGATGAC 
GGACCCAAAG GAAACCCTGG TCCTGTTGGA TTCCCTGGTG ATCCTGGACC CCCTGGTGAA 
GTTGGACCAC GAGGTCAAGA TGGTGCAAAG GGTGACAGAG GAGAGGATGG AGAACAAGGA 
GAAGCCGGCT CACCTGGACC TACTGGAGAG AACGGACCAC CTGGACCTCC AGGAAAGAGA 
GGTCCTGCAG GCACAAGGGG ACCGGAGGGC CGTCAAGGAG AGAAAGGAAG CAAGGGTGAT 
TCAGGTGCTC TTGGTCCTCC CGGAAAAACT GGCCCTGTGG GGCCACAGGG ACAACCCGGA 
AAACCAGGAA CTGAGGGTCT AAGAGGGTTG CCTGGAACAG TGGGTGAGCA AGGTTCTCCA 
GGTGCTGCAG GCCCTAAAGG ACCTCCTGGA CCTCTGGGTC CTCCAGGTCT GGCTGGTCTT 
CGCGGTGATC CCGGTGCTAA AGGTGAGAAG GGACATCCAG GTATGCTCGG ACTGATCGGG 
CCTGCTGGAG AACAAGGAGA GAAGGGAGAC AGAGGCATGC CAGGACCACA GGGATCAACA 
GGACCCAAGG GAGAAACGGG TATATCTGGA GGAACTGGTC CACTTGGTCC TGCAGGTCCT 
GCTGGTATGC CTGGCCCGAG GGGTGTCAAA GGAGCTAAAG GTGCCAGTGG TGGGGCTGGT 
CCTAAAGGAG AAAAGGGTGT ACAAGGACCA CCAGGACCAC CTGGTCCCCC TGGTGATGTC 
ATCCAGCCGC TGCCCATTCA GATCCCCAAG AAGTCCAAGC GCTCCATCGA CGCCAGTAAG 
ATTCTGTCTG AGGCTGACGC TGAGGCTGCC GACACCACTG GCTCTGAGTT CCTGACTGGT 
AATGAAGGAA TGGAGGAGAT CTTCGGCTCA CTTAATTCCC TGCGCCAGGA GATCGAGAGC 
ATGCGCTTCC CATTGGGCAC AAAGGAGAGC CCCGGCCGCA CCTGCCAGGA TCTCCATCTC 
AGCCAACCAG ATCTGAAAGA CGGCGAGTAT TGGATTGATC CAAATCAAGG CTGTGCTCGC 
GACTCTTTCA AGGTTTACTG TAACTTCACT GCCGGCGGAG AGACCTGCCT GTATCCCAGC 
AAAGCAGTGG AGAATGTAAA GATGAACTCT TGGATAAAGG AGAAGCCTGG GTCCTTGTTC 
AGTCAGTTTG CAAAGGGCAA CAAGTTTTCA TATGTAGACT CGGTTGGAGA GGCTGTGGGT 
GTTGTTCAGC TGGGCTTCTT GCGGCTGCTC AGTGTCCAGG CCAGACAGAA CCTCACCTAC 
CACTGCCAAC GCTCGGTGGC CTGGACTGAC CAAACCACCG ACGGCGGATA CAAGCGGGCG 
CTGCGTCTGC AGGGGGCCAA TGATGAGGAG ATCAGCTATG AGACTAGCCC ATATATCAAA 
GCCCTCATCG ACGGCTGCTC CTATCGTAAG GGTCTGGACA GGACGGTGCT GGAGGTGAAC 
ACACCTCAGG TGGAGCAGCT CCCTCTGCTG GACATCAGGA TCTCAGACTT TGGGGAGGAC 
AATCAGAAAT TTGGTTTTGA AGTAGGACCT GTCTGTTTCC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_017352252.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207741.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.

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CloneID	ODa56071
Clone ID Related Accession (Same CDS sequence)	XM_017352252.1 , XM_017352252.2
Accession Version	XM_017352252.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(XI) chain isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGTCGACGA GACTGTCCCC GTTGAGGAAA TGGATGCTAC CAAGACAGGT 
GGTGAGGTGG CGTCTCAAAC GCCAGAAGAG CCCTTCACTG AGGAGTACAT TACAAGCGAC 
TTTGGGATGA AGGAGTATGA CTATTCCTAC AAGGACTACA ATGAGCCCAT CATTGAGAGC 
AAGACGGAGG ACACTCTAGG CCCTGCCCTG TCCGCTGTGA CAGAGGAGGG GGGTGCCTCA 
GTGCGAGCCA TTAAGGGAGA GAAGGGAGAG CCTGCTGTCC TTGAACCTGG CATGCTGATT 
GAAGGACCAC CAGGACCAGA AGGACCAGCA GGTCTCCCTG GCCCCTCTGG CCCCCAAGGA 
CAACCTGGTT CTGTTGGTGA TCCTGGTGAG AGGGGTCCTA CTGGTAAGGC TGGTCTACCT 
GGAGCAGATG GTGTTCCAGG ACCTCCAGGA ACCTCTGTCA TGCTTCCTTT CCGCTTTGGA 
CAGAGCGCTG GAGAGAAAGG ACCTGTTGCC TCTGCACAGG AAGCCCAAGC TCAAGCCATT 
CTGTCTCAAG CTCGGCTTGC CCTGAAAGGA CCATCCGGGC CAATGGGTTT CACTGGCCGT 
CCTGGACCTT TGGGAACACC TGGAAGTCCT GGCTTTAAGG GAGAAAGGGG GGACCCTGGT 
GCTCAGGGTC CTAGAGGTCC TCAGGGTGTG TCTGGTCCTC CTGGCAAAGC TGGTCGGAGG 
GGTCGCGCTG GTGCTGATGG AGCCAGAGGG ATGCCAGGAG AGTCTGGAAT TAAGGGTGAC 
CGTGGTTTTG ATGGCCTTCC TGGTTTGCCT GGTGACAAGG GTTATCGGGG TCAACCTGGA 
GGAATGGGAC CCCCAGGACC ACATGGAGAG GACGGAGAGA GGGGAGATGA TGGAGATGTT 
GGACCCAGAG GTCTTCCTGG CGAACCTGGA CCCCGTGGTT TGTTGGGGCC TAAAGGGCCT 
TCTGGACTTC CTGGACCTCC TGGTGTTCGT GGAAATGATG GTCCACATGG TCCAAAAGGA 
AACTTAGGAC CACAAGGAGA GCCCGGTCCT CCAGGCCAGC AGGGGGCTCC AGGAACACAG 
GGAATGCCAG GTCCACAGGG TGCCACTGGA CCACCAGGAG AGAAAGGACC CACAGGAAAG 
CCAGGTCTAC CAGGAATGCC AGGAGCTGAT GGTCCTCCCG GTCATCCCGG GAAGGAAGGT 
CCTGGTGGAA CCAAAGGAAA TCAAGGTCCT AATGGTCCTC AGGGCTCTAT TGGCTATCCT 
GGCCCTCGGG GATTGAAGGG AGCTCAAGGT ATCCGTGGAC TGAAAGGTCA TAAAGGAGAG 
AAGGGAGAGG ATGGATTCCC AGGAATTAAA GGAGACTTTG GTGTGAAGGG AGAGAGGGGA 
GAGGTGGGCG TGCCCGGCCC AAGAGGAGAA GATGGTCCTG AGGGGCCAAA GGGTCGCGTC 
GGACCCCCTG GTGAAATTGG TCCCCTCGGC CTGTTGGGAG AGAAGGGTAA ACTTGGTGTT 
CCTGGACTTC CTGGATATCC TGGACGTCAA GGAATAAAGG GGTCCCTTGG TTTCCCAGGA 
TTCCCTGGTA CAAATGGCGA GAAGGGCACA AGGGGTTTGA TTGGAAAACC TGGACCAAGA 
GGACAAAGAG GGCCGACAGG TCCTAGAGGG CAGAGAGGAC CACGTGGGGC AACTGGGAAA 
TCAGGTGCTA AGGGTGGATC AGGAAGTGAC GGGCCTCCTG GACCACCAGG AGAGAGAGGG 
CTAACTGGAC CTCAGGGTGC TAATGGATTC CCTGGGCCAA AGGGACCTCC CGGACCTCCA 
GGGAAAGATG GACTTCCTGG ACACCCTGGA CAGAGAGGCG AAGTCGGTTT CCAAGGCAAA 
GTTGGACCAC CTGGCCCACC TGGAGTTGTT GGCCCACACG GTCCCTCTGG TGAGACTGGT 
CAGATGGGTG AGCGTGGCCA CCCTGGACCT CCAGGACCAC CTGGTGAGCA AGGCCTTTCT 
GGCTCCTCTG GTAAAGAAGG AACCAAAGGA GATCCCGGTC CTCCTGGTGG CCCCGGTAAA 
GATGGACCCC CTGGACTTAG AGGATTCCCA GGAGAGAGAG GACTACCAGG AACTCCAGGT 
TCTGGAGGAC TGAAAGGAAA TGAAGGCCCT GCTGGACCAC CTGGTCCTGC GGGTTCATCT 
GGGGAGAGAG GTGGTGCTGG CACTGCAGGC CCTGTCGGCC CACCTGGACG ACCTGGACCT 
CAGGGACCCC CTGGAACTTC AGGTGAAAAG GGAGTACCAG GCGAGAAAGG TCCGGTTGGT 
CCAGCTGGTC GTGATGGCAT CCAAGGCCCA GTTGGTCTCC CAGGCCCTGC CGGACCTCCA 
GGTATTTCTG GAGAAGATGG AGACAAGGGT GAGGTTGGAG AGCCAGGACA AAAGGGAGCT 
AAAGGGTCCA AAGGAGAACA TGGTCCTCCT GGTCCACCTG GGCCGATGGG TCCTGTTGGA 
CAGCCTGGTG CAGCGGGTGC TGATGGTGAG ACGGGTCCAA GAGGTCAACA GGGACAGTTT 
GGTGCCAAGG GAGATGAAGG CACAAGAGGT TTCCCTGGTC CCCCTGGCCC CATAGGATTG 
CAGGGTTTGC CAGGCCCAGG TGGAGAAAAG GGAGAGACAG GAGATGTTGG ACCCTTGGGT 
CCTCCTGGTC CTCCAGGGCC GCGTGGTCCT GCTGGTCCTA ATGGTGCTGA TGGTCCCCAA 
GGTCCTCCAG GAGGTTTGGG TAATCCAGGT CCCATAGGAG AGAAGGGTGA GCCTGGTGAA 
TCTGGACCAC CTGGTGTTGG TGGAGAGCCA GGAAAATTGG GCCCAAGAGG AGAGCGTGGT 
GAGAAGGGAG AGTCCGGGCA GCCTGGCACC CCTGGTCCTC CTGGAGGAAA GGGACCCACT 
GGAGATGACG GACCCAAAGG AAACCCTGGT CCTGTTGGAT TCCCTGGTGA TCCTGGACCC 
CCTGGTGAAG TTGGACCACG AGGTCAAGAT GGTGCAAAGG GTGACAGAGG AGAGGATGGA 
GAACAAGGAG AAGCCGGCTC ACCTGGACCT ACTGGAGAGA ACGGACCACC TGGACCTCCA 
GGAAAGAGAG GTCCTGCAGG CACAAGGGGA CCGGAGGGCC GTCAAGGAGA GAAAGGAAGC 
AAGGGTGATT CAGGTGCTCT TGGTCCTCCC GGAAAAACTG GCCCTGTGGG GCCACAGGGA 
CAACCCGGAA AACCAGGAAC TGAGGGTCTA AGAGGGTTGC CTGGAACAGT GGGTGAGCAA 
GGTTCTCCAG GTGCTGCAGG CCCTAAAGGA CCTCCTGGAC CTCTGGGTCC TCCAGGTCTG 
GCTGGTCTTC GCGGTGATCC CGGTGCTAAA GGTGAGAAGG GACATCCAGG TATGCTCGGA 
CTGATCGGGC CTGCTGGAGA ACAAGGAGAG AAGGGAGACA GAGGCATGCC AGGACCACAG 
GGATCAACAG GACCCAAGGG AGAAACGGGT ATATCTGGAG GAACTGGTCC ACTTGGTCCT 
GCAGGTCCTG CTGGTATGCC TGGCCCGAGG GGTGTCAAAG GAGCTAAAGG TGCCAGTGGT 
GGGGCTGGTC CTAAAGGAGA AAAGGGTGTA CAAGGACCAC CAGGACCACC TGGTCCCCCT 
GGTGATGTCA TCCAGCCGCT GCCCATTCAG ATCCCCAAGA AGTCCAAGCG CTCCATCGAC 
GCCAGTAAGA TTCTGTCTGA GGCTGACGCT GAGGCTGCCG ACACCACTGG CTCTGAGTTC 
CTGACTGGTA ATGAAGGAAT GGAGGAGATC TTCGGCTCAC TTAATTCCCT GCGCCAGGAG 
ATCGAGAGCA TGCGCTTCCC ATTGGGCACA AAGGAGAGCC CCGGCCGCAC CTGCCAGGAT 
CTCCATCTCA GCCAACCAGA TCTGAAAGAC GGCGAGTATT GGATTGATCC AAATCAAGGC 
TGTGCTCGCG ACTCTTTCAA GGTTTACTGT AACTTCACTG CCGGCGGAGA GACCTGCCTG 
TATCCCAGCA AAGCAGTGGA GAATGTAAAG ATGAACTCTT GGATAAAGGA GAAGCCTGGG 
TCCTTGTTCA GTCAGTTTGC AAAGGGCAAC AAGTTTTCAT ATGTAGACTC GGTTGGAGAG 
GCTGTGGGTG TTGTTCAGCT GGGCTTCTTG CGGCTGCTCA GTGTCCAGGC CAGACAGAAC 
CTCACCTACC ACTGCCAACG CTCGGTGGCC TGGACTGACC AAACCACCGA CGGCGGATAC 
AAGCGGGCGC TGCGTCTGCA GGGGGCCAAT GATGAGGAGA TCAGCTATGA GACTAGCCCA 
TATATCAAAG CCCTCATCGA CGGCTGCTCC TATCGTAAGG GTCTGGACAG GACGGTGCTG 
GAGGTGAACA CACCTCAGGT GGAGCAGCTC CCTCTGCTGG ACATCAGGAT CTCAGACTTT 
GGGGAGGACA ATCAGAAATT TGGTTTTGAA GTAGGACCTG TCTGTTTCCT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207741.1
CDS	384..6320
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYAGKANNGKVTKQKTAPAAKPAPAKPAKSKPTPAPKTAKSKPTAAQLLISKIKPTPASKLAAKPKATPAPKNDKKKTNDNGKVNNGQTKVNGNGNGKSNGNGKDTNGKAKAGSNGKPNNNGNGNGKKTNGDAKINGNGPKTVKTVTKVASAKKSETPATKTVTGKPKAQVKAQPTKSKTDKTKANASPAKPKDTKAKAASVTQKPAVFKPTPVPLPRPSKTILDVNNVKSMSRNFPPFDKAVVSATTIKKKPTNGYQQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_017352252.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352252.1

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGTCGACGA GACTGTCCCC GTTGAGGAAA TGGATGCTAC CAAGACAGGT 
GGTGAGGTGG CGTCTCAAAC GCCAGAAGAG CCCTTCACTG AGGAGTACAT TACAAGCGAC 
TTTGGGATGA AGGAGTATGA CTATTCCTAC AAGGACTACA ATGAGCCCAT CATTGAGAGC 
AAGACGGAGG ACACTCTAGG CCCTGCCCTG TCCGCTGTGA CAGAGGAGGG GGGTGCCTCA 
GTGCGAGCCA TTAAGGGAGA GAAGGGAGAG CCTGCTGTCC TTGAACCTGG CATGCTGATT 
GAAGGACCAC CAGGACCAGA AGGACCAGCA GGTCTCCCTG GCCCCTCTGG CCCCCAAGGA 
CAACCTGGTT CTGTTGGTGA TCCTGGTGAG AGGGGTCCTA CTGGTAAGGC TGGTCTACCT 
GGAGCAGATG GTGTTCCAGG ACCTCCAGGA ACCTCTGTCA TGCTTCCTTT CCGCTTTGGA 
CAGAGCGCTG GAGAGAAAGG ACCTGTTGCC TCTGCACAGG AAGCCCAAGC TCAAGCCATT 
CTGTCTCAAG CTCGGCTTGC CCTGAAAGGA CCATCCGGGC CAATGGGTTT CACTGGCCGT 
CCTGGACCTT TGGGAACACC TGGAAGTCCT GGCTTTAAGG GAGAAAGGGG GGACCCTGGT 
GCTCAGGGTC CTAGAGGTCC TCAGGGTGTG TCTGGTCCTC CTGGCAAAGC TGGTCGGAGG 
GGTCGCGCTG GTGCTGATGG AGCCAGAGGG ATGCCAGGAG AGTCTGGAAT TAAGGGTGAC 
CGTGGTTTTG ATGGCCTTCC TGGTTTGCCT GGTGACAAGG GTTATCGGGG TCAACCTGGA 
GGAATGGGAC CCCCAGGACC ACATGGAGAG GACGGAGAGA GGGGAGATGA TGGAGATGTT 
GGACCCAGAG GTCTTCCTGG CGAACCTGGA CCCCGTGGTT TGTTGGGGCC TAAAGGGCCT 
TCTGGACTTC CTGGACCTCC TGGTGTTCGT GGAAATGATG GTCCACATGG TCCAAAAGGA 
AACTTAGGAC CACAAGGAGA GCCCGGTCCT CCAGGCCAGC AGGGGGCTCC AGGAACACAG 
GGAATGCCAG GTCCACAGGG TGCCACTGGA CCACCAGGAG AGAAAGGACC CACAGGAAAG 
CCAGGTCTAC CAGGAATGCC AGGAGCTGAT GGTCCTCCCG GTCATCCCGG GAAGGAAGGT 
CCTGGTGGAA CCAAAGGAAA TCAAGGTCCT AATGGTCCTC AGGGCTCTAT TGGCTATCCT 
GGCCCTCGGG GATTGAAGGG AGCTCAAGGT ATCCGTGGAC TGAAAGGTCA TAAAGGAGAG 
AAGGGAGAGG ATGGATTCCC AGGAATTAAA GGAGACTTTG GTGTGAAGGG AGAGAGGGGA 
GAGGTGGGCG TGCCCGGCCC AAGAGGAGAA GATGGTCCTG AGGGGCCAAA GGGTCGCGTC 
GGACCCCCTG GTGAAATTGG TCCCCTCGGC CTGTTGGGAG AGAAGGGTAA ACTTGGTGTT 
CCTGGACTTC CTGGATATCC TGGACGTCAA GGAATAAAGG GGTCCCTTGG TTTCCCAGGA 
TTCCCTGGTA CAAATGGCGA GAAGGGCACA AGGGGTTTGA TTGGAAAACC TGGACCAAGA 
GGACAAAGAG GGCCGACAGG TCCTAGAGGG CAGAGAGGAC CACGTGGGGC AACTGGGAAA 
TCAGGTGCTA AGGGTGGATC AGGAAGTGAC GGGCCTCCTG GACCACCAGG AGAGAGAGGG 
CTAACTGGAC CTCAGGGTGC TAATGGATTC CCTGGGCCAA AGGGACCTCC CGGACCTCCA 
GGGAAAGATG GACTTCCTGG ACACCCTGGA CAGAGAGGCG AAGTCGGTTT CCAAGGCAAA 
GTTGGACCAC CTGGCCCACC TGGAGTTGTT GGCCCACACG GTCCCTCTGG TGAGACTGGT 
CAGATGGGTG AGCGTGGCCA CCCTGGACCT CCAGGACCAC CTGGTGAGCA AGGCCTTTCT 
GGCTCCTCTG GTAAAGAAGG AACCAAAGGA GATCCCGGTC CTCCTGGTGG CCCCGGTAAA 
GATGGACCCC CTGGACTTAG AGGATTCCCA GGAGAGAGAG GACTACCAGG AACTCCAGGT 
TCTGGAGGAC TGAAAGGAAA TGAAGGCCCT GCTGGACCAC CTGGTCCTGC GGGTTCATCT 
GGGGAGAGAG GTGGTGCTGG CACTGCAGGC CCTGTCGGCC CACCTGGACG ACCTGGACCT 
CAGGGACCCC CTGGAACTTC AGGTGAAAAG GGAGTACCAG GCGAGAAAGG TCCGGTTGGT 
CCAGCTGGTC GTGATGGCAT CCAAGGCCCA GTTGGTCTCC CAGGCCCTGC CGGACCTCCA 
GGTATTTCTG GAGAAGATGG AGACAAGGGT GAGGTTGGAG AGCCAGGACA AAAGGGAGCT 
AAAGGGTCCA AAGGAGAACA TGGTCCTCCT GGTCCACCTG GGCCGATGGG TCCTGTTGGA 
CAGCCTGGTG CAGCGGGTGC TGATGGTGAG ACGGGTCCAA GAGGTCAACA GGGACAGTTT 
GGTGCCAAGG GAGATGAAGG CACAAGAGGT TTCCCTGGTC CCCCTGGCCC CATAGGATTG 
CAGGGTTTGC CAGGCCCAGG TGGAGAAAAG GGAGAGACAG GAGATGTTGG ACCCTTGGGT 
CCTCCTGGTC CTCCAGGGCC GCGTGGTCCT GCTGGTCCTA ATGGTGCTGA TGGTCCCCAA 
GGTCCTCCAG GAGGTTTGGG TAATCCAGGT CCCATAGGAG AGAAGGGTGA GCCTGGTGAA 
TCTGGACCAC CTGGTGTTGG TGGAGAGCCA GGAAAATTGG GCCCAAGAGG AGAGCGTGGT 
GAGAAGGGAG AGTCCGGGCA GCCTGGCACC CCTGGTCCTC CTGGAGGAAA GGGACCCACT 
GGAGATGACG GACCCAAAGG AAACCCTGGT CCTGTTGGAT TCCCTGGTGA TCCTGGACCC 
CCTGGTGAAG TTGGACCACG AGGTCAAGAT GGTGCAAAGG GTGACAGAGG AGAGGATGGA 
GAACAAGGAG AAGCCGGCTC ACCTGGACCT ACTGGAGAGA ACGGACCACC TGGACCTCCA 
GGAAAGAGAG GTCCTGCAGG CACAAGGGGA CCGGAGGGCC GTCAAGGAGA GAAAGGAAGC 
AAGGGTGATT CAGGTGCTCT TGGTCCTCCC GGAAAAACTG GCCCTGTGGG GCCACAGGGA 
CAACCCGGAA AACCAGGAAC TGAGGGTCTA AGAGGGTTGC CTGGAACAGT GGGTGAGCAA 
GGTTCTCCAG GTGCTGCAGG CCCTAAAGGA CCTCCTGGAC CTCTGGGTCC TCCAGGTCTG 
GCTGGTCTTC GCGGTGATCC CGGTGCTAAA GGTGAGAAGG GACATCCAGG TATGCTCGGA 
CTGATCGGGC CTGCTGGAGA ACAAGGAGAG AAGGGAGACA GAGGCATGCC AGGACCACAG 
GGATCAACAG GACCCAAGGG AGAAACGGGT ATATCTGGAG GAACTGGTCC ACTTGGTCCT 
GCAGGTCCTG CTGGTATGCC TGGCCCGAGG GGTGTCAAAG GAGCTAAAGG TGCCAGTGGT 
GGGGCTGGTC CTAAAGGAGA AAAGGGTGTA CAAGGACCAC CAGGACCACC TGGTCCCCCT 
GGTGATGTCA TCCAGCCGCT GCCCATTCAG ATCCCCAAGA AGTCCAAGCG CTCCATCGAC 
GCCAGTAAGA TTCTGTCTGA GGCTGACGCT GAGGCTGCCG ACACCACTGG CTCTGAGTTC 
CTGACTGGTA ATGAAGGAAT GGAGGAGATC TTCGGCTCAC TTAATTCCCT GCGCCAGGAG 
ATCGAGAGCA TGCGCTTCCC ATTGGGCACA AAGGAGAGCC CCGGCCGCAC CTGCCAGGAT 
CTCCATCTCA GCCAACCAGA TCTGAAAGAC GGCGAGTATT GGATTGATCC AAATCAAGGC 
TGTGCTCGCG ACTCTTTCAA GGTTTACTGT AACTTCACTG CCGGCGGAGA GACCTGCCTG 
TATCCCAGCA AAGCAGTGGA GAATGTAAAG ATGAACTCTT GGATAAAGGA GAAGCCTGGG 
TCCTTGTTCA GTCAGTTTGC AAAGGGCAAC AAGTTTTCAT ATGTAGACTC GGTTGGAGAG 
GCTGTGGGTG TTGTTCAGCT GGGCTTCTTG CGGCTGCTCA GTGTCCAGGC CAGACAGAAC 
CTCACCTACC ACTGCCAACG CTCGGTGGCC TGGACTGACC AAACCACCGA CGGCGGATAC 
AAGCGGGCGC TGCGTCTGCA GGGGGCCAAT GATGAGGAGA TCAGCTATGA GACTAGCCCA 
TATATCAAAG CCCTCATCGA CGGCTGCTCC TATCGTAAGG GTCTGGACAG GACGGTGCTG 
GAGGTGAACA CACCTCAGGT GGAGCAGCTC CCTCTGCTGG ACATCAGGAT CTCAGACTTT 
GGGGAGGACA ATCAGAAATT TGGTTTTGAA GTAGGACCTG TCTGTTTCCT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_017352252.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207741.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.

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CloneID	ODa56071
Clone ID Related Accession (Same CDS sequence)	XM_017352252.1 , XM_017352252.2
Accession Version	XM_017352252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGTCGACGA GACTGTCCCC GTTGAGGAAA TGGATGCTAC CAAGACAGGT 
GGTGAGGTGG CGTCTCAAAC GCCAGAAGAG CCCTTCACTG AGGAGTACAT TACAAGCGAC 
TTTGGGATGA AGGAGTATGA CTATTCCTAC AAGGACTACA ATGAGCCCAT CATTGAGAGC 
AAGACGGAGG ACACTCTAGG CCCTGCCCTG TCCGCTGTGA CAGAGGAGGG GGGTGCCTCA 
GTGCGAGCCA TTAAGGGAGA GAAGGGAGAG CCTGCTGTCC TTGAACCTGG CATGCTGATT 
GAAGGACCAC CAGGACCAGA AGGACCAGCA GGTCTCCCTG GCCCCTCTGG CCCCCAAGGA 
CAACCTGGTT CTGTTGGTGA TCCTGGTGAG AGGGGTCCTA CTGGTAAGGC TGGTCTACCT 
GGAGCAGATG GTGTTCCAGG ACCTCCAGGA ACCTCTGTCA TGCTTCCTTT CCGCTTTGGA 
CAGAGCGCTG GAGAGAAAGG ACCTGTTGCC TCTGCACAGG AAGCCCAAGC TCAAGCCATT 
CTGTCTCAAG CTCGGCTTGC CCTGAAAGGA CCATCCGGGC CAATGGGTTT CACTGGCCGT 
CCTGGACCTT TGGGAACACC TGGAAGTCCT GGCTTTAAGG GAGAAAGGGG GGACCCTGGT 
GCTCAGGGTC CTAGAGGTCC TCAGGGTGTG TCTGGTCCTC CTGGCAAAGC TGGTCGGAGG 
GGTCGCGCTG GTGCTGATGG AGCCAGAGGG ATGCCAGGAG AGTCTGGAAT TAAGGGTGAC 
CGTGGTTTTG ATGGCCTTCC TGGTTTGCCT GGTGACAAGG GTTATCGGGG TCAACCTGGA 
GGAATGGGAC CCCCAGGACC ACATGGAGAG GACGGAGAGA GGGGAGATGA TGGAGATGTT 
GGACCCAGAG GTCTTCCTGG CGAACCTGGA CCCCGTGGTT TGTTGGGGCC TAAAGGGCCT 
TCTGGACTTC CTGGACCTCC TGGTGTTCGT GGAAATGATG GTCCACATGG TCCAAAAGGA 
AACTTAGGAC CACAAGGAGA GCCCGGTCCT CCAGGCCAGC AGGGGGCTCC AGGAACACAG 
GGAATGCCAG GTCCACAGGG TGCCACTGGA CCACCAGGAG AGAAAGGACC CACAGGAAAG 
CCAGGTCTAC CAGGAATGCC AGGAGCTGAT GGTCCTCCCG GTCATCCCGG GAAGGAAGGT 
CCTGGTGGAA CCAAAGGAAA TCAAGGTCCT AATGGTCCTC AGGGCTCTAT TGGCTATCCT 
GGCCCTCGGG GATTGAAGGG AGCTCAAGGT ATCCGTGGAC TGAAAGGTCA TAAAGGAGAG 
AAGGGAGAGG ATGGATTCCC AGGAATTAAA GGAGACTTTG GTGTGAAGGG AGAGAGGGGA 
GAGGTGGGCG TGCCCGGCCC AAGAGGAGAA GATGGTCCTG AGGGGCCAAA GGGTCGCGTC 
GGACCCCCTG GTGAAATTGG TCCCCTCGGC CTGTTGGGAG AGAAGGGTAA ACTTGGTGTT 
CCTGGACTTC CTGGATATCC TGGACGTCAA GGAATAAAGG GGTCCCTTGG TTTCCCAGGA 
TTCCCTGGTA CAAATGGCGA GAAGGGCACA AGGGGTTTGA TTGGAAAACC TGGACCAAGA 
GGACAAAGAG GGCCGACAGG TCCTAGAGGG CAGAGAGGAC CACGTGGGGC AACTGGGAAA 
TCAGGTGCTA AGGGTGGATC AGGAAGTGAC GGGCCTCCTG GACCACCAGG AGAGAGAGGG 
CTAACTGGAC CTCAGGGTGC TAATGGATTC CCTGGGCCAA AGGGACCTCC CGGACCTCCA 
GGGAAAGATG GACTTCCTGG ACACCCTGGA CAGAGAGGCG AAGTCGGTTT CCAAGGCAAA 
GTTGGACCAC CTGGCCCACC TGGAGTTGTT GGCCCACACG GTCCCTCTGG TGAGACTGGT 
CAGATGGGTG AGCGTGGCCA CCCTGGACCT CCAGGACCAC CTGGTGAGCA AGGCCTTTCT 
GGCTCCTCTG GTAAAGAAGG AACCAAAGGA GATCCCGGTC CTCCTGGTGG CCCCGGTAAA 
GATGGACCCC CTGGACTTAG AGGATTCCCA GGAGAGAGAG GACTACCAGG AACTCCAGGT 
TCTGGAGGAC TGAAAGGAAA TGAAGGCCCT GCTGGACCAC CTGGTCCTGC GGGTTCATCT 
GGGGAGAGAG GTGGTGCTGG CACTGCAGGC CCTGTCGGCC CACCTGGACG ACCTGGACCT 
CAGGGACCCC CTGGAACTTC AGGTGAAAAG GGAGTACCAG GCGAGAAAGG TCCGGTTGGT 
CCAGCTGGTC GTGATGGCAT CCAAGGCCCA GTTGGTCTCC CAGGCCCTGC CGGACCTCCA 
GGTATTTCTG GAGAAGATGG AGACAAGGGT GAGGTTGGAG AGCCAGGACA AAAGGGAGCT 
AAAGGGTCCA AAGGAGAACA TGGTCCTCCT GGTCCACCTG GGCCGATGGG TCCTGTTGGA 
CAGCCTGGTG CAGCGGGTGC TGATGGTGAG ACGGGTCCAA GAGGTCAACA GGGACAGTTT 
GGTGCCAAGG GAGATGAAGG CACAAGAGGT TTCCCTGGTC CCCCTGGCCC CATAGGATTG 
CAGGGTTTGC CAGGCCCAGG TGGAGAAAAG GGAGAGACAG GAGATGTTGG ACCCTTGGGT 
CCTCCTGGTC CTCCAGGGCC GCGTGGTCCT GCTGGTCCTA ATGGTGCTGA TGGTCCCCAA 
GGTCCTCCAG GAGGTTTGGG TAATCCAGGT CCCATAGGAG AGAAGGGTGA GCCTGGTGAA 
TCTGGACCAC CTGGTGTTGG TGGAGAGCCA GGAAAATTGG GCCCAAGAGG AGAGCGTGGT 
GAGAAGGGAG AGTCCGGGCA GCCTGGCACC CCTGGTCCTC CTGGAGGAAA GGGACCCACT 
GGAGATGACG GACCCAAAGG AAACCCTGGT CCTGTTGGAT TCCCTGGTGA TCCTGGACCC 
CCTGGTGAAG TTGGACCACG AGGTCAAGAT GGTGCAAAGG GTGACAGAGG AGAGGATGGA 
GAACAAGGAG AAGCCGGCTC ACCTGGACCT ACTGGAGAGA ACGGACCACC TGGACCTCCA 
GGAAAGAGAG GTCCTGCAGG CACAAGGGGA CCGGAGGGCC GTCAAGGAGA GAAAGGAAGC 
AAGGGTGATT CAGGTGCTCT TGGTCCTCCC GGAAAAACTG GCCCTGTGGG GCCACAGGGA 
CAACCCGGAA AACCAGGAAC TGAGGGTCTA AGAGGGTTGC CTGGAACAGT GGGTGAGCAA 
GGTTCTCCAG GTGCTGCAGG CCCTAAAGGA CCTCCTGGAC CTCTGGGTCC TCCAGGTCTG 
GCTGGTCTTC GCGGTGATCC CGGTGCTAAA GGTGAGAAGG GACATCCAGG TATGCTCGGA 
CTGATCGGGC CTGCTGGAGA ACAAGGAGAG AAGGGAGACA GAGGCATGCC AGGACCACAG 
GGATCAACAG GACCCAAGGG AGAAACGGGT ATATCTGGAG GAACTGGTCC ACTTGGTCCT 
GCAGGTCCTG CTGGTATGCC TGGCCCGAGG GGTGTCAAAG GAGCTAAAGG TGCCAGTGGT 
GGGGCTGGTC CTAAAGGAGA AAAGGGTGTA CAAGGACCAC CAGGACCACC TGGTCCCCCT 
GGTGATGTCA TCCAGCCGCT GCCCATTCAG ATCCCCAAGA AGTCCAAGCG CTCCATCGAC 
GCCAGTAAGA TTCTGTCTGA GGCTGACGCT GAGGCTGCCG ACACCACTGG CTCTGAGTTC 
CTGACTGGTA ATGAAGGAAT GGAGGAGATC TTCGGCTCAC TTAATTCCCT GCGCCAGGAG 
ATCGAGAGCA TGCGCTTCCC ATTGGGCACA AAGGAGAGCC CCGGCCGCAC CTGCCAGGAT 
CTCCATCTCA GCCAACCAGA TCTGAAAGAC GGCGAGTATT GGATTGATCC AAATCAAGGC 
TGTGCTCGCG ACTCTTTCAA GGTTTACTGT AACTTCACTG CCGGCGGAGA GACCTGCCTG 
TATCCCAGCA AAGCAGTGGA GAATGTAAAG ATGAACTCTT GGATAAAGGA GAAGCCTGGG 
TCCTTGTTCA GTCAGTTTGC AAAGGGCAAC AAGTTTTCAT ATGTAGACTC GGTTGGAGAG 
GCTGTGGGTG TTGTTCAGCT GGGCTTCTTG CGGCTGCTCA GTGTCCAGGC CAGACAGAAC 
CTCACCTACC ACTGCCAACG CTCGGTGGCC TGGACTGACC AAACCACCGA CGGCGGATAC 
AAGCGGGCGC TGCGTCTGCA GGGGGCCAAT GATGAGGAGA TCAGCTATGA GACTAGCCCA 
TATATCAAAG CCCTCATCGA CGGCTGCTCC TATCGTAAGG GTCTGGACAG GACGGTGCTG 
GAGGTGAACA CACCTCAGGT GGAGCAGCTC CCTCTGCTGG ACATCAGGAT CTCAGACTTT 
GGGGAGGACA ATCAGAAATT TGGTTTTGAA GTAGGACCTG TCTGTTTCCT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207741.1
CDS	395..6331
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYAGKANNGKVTKQKTAPAAKPAPAKPAKSKPTPAPKTAKSKPTAAQLLISKIKPTPASKLAAKPKATPAPKNDKKKTNDNGKVNNGQTKVNGNGNGKSNGNGKDTNGKAKAGSNGKPNNNGNGNGKKTNGDAKINGNGPKTVKTVTKVASAKKSETPATKTVTGKPKAQVKAQPTKSKTDKTKANASPAKPKDTKAKAASVTQKPAVFKPTPVPLPRPSKTILDVNNVKSMSRNFPPFDKAVVSATTIKKKPTNGYQQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_017352252.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352252.2

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGTCGACGA GACTGTCCCC GTTGAGGAAA TGGATGCTAC CAAGACAGGT 
GGTGAGGTGG CGTCTCAAAC GCCAGAAGAG CCCTTCACTG AGGAGTACAT TACAAGCGAC 
TTTGGGATGA AGGAGTATGA CTATTCCTAC AAGGACTACA ATGAGCCCAT CATTGAGAGC 
AAGACGGAGG ACACTCTAGG CCCTGCCCTG TCCGCTGTGA CAGAGGAGGG GGGTGCCTCA 
GTGCGAGCCA TTAAGGGAGA GAAGGGAGAG CCTGCTGTCC TTGAACCTGG CATGCTGATT 
GAAGGACCAC CAGGACCAGA AGGACCAGCA GGTCTCCCTG GCCCCTCTGG CCCCCAAGGA 
CAACCTGGTT CTGTTGGTGA TCCTGGTGAG AGGGGTCCTA CTGGTAAGGC TGGTCTACCT 
GGAGCAGATG GTGTTCCAGG ACCTCCAGGA ACCTCTGTCA TGCTTCCTTT CCGCTTTGGA 
CAGAGCGCTG GAGAGAAAGG ACCTGTTGCC TCTGCACAGG AAGCCCAAGC TCAAGCCATT 
CTGTCTCAAG CTCGGCTTGC CCTGAAAGGA CCATCCGGGC CAATGGGTTT CACTGGCCGT 
CCTGGACCTT TGGGAACACC TGGAAGTCCT GGCTTTAAGG GAGAAAGGGG GGACCCTGGT 
GCTCAGGGTC CTAGAGGTCC TCAGGGTGTG TCTGGTCCTC CTGGCAAAGC TGGTCGGAGG 
GGTCGCGCTG GTGCTGATGG AGCCAGAGGG ATGCCAGGAG AGTCTGGAAT TAAGGGTGAC 
CGTGGTTTTG ATGGCCTTCC TGGTTTGCCT GGTGACAAGG GTTATCGGGG TCAACCTGGA 
GGAATGGGAC CCCCAGGACC ACATGGAGAG GACGGAGAGA GGGGAGATGA TGGAGATGTT 
GGACCCAGAG GTCTTCCTGG CGAACCTGGA CCCCGTGGTT TGTTGGGGCC TAAAGGGCCT 
TCTGGACTTC CTGGACCTCC TGGTGTTCGT GGAAATGATG GTCCACATGG TCCAAAAGGA 
AACTTAGGAC CACAAGGAGA GCCCGGTCCT CCAGGCCAGC AGGGGGCTCC AGGAACACAG 
GGAATGCCAG GTCCACAGGG TGCCACTGGA CCACCAGGAG AGAAAGGACC CACAGGAAAG 
CCAGGTCTAC CAGGAATGCC AGGAGCTGAT GGTCCTCCCG GTCATCCCGG GAAGGAAGGT 
CCTGGTGGAA CCAAAGGAAA TCAAGGTCCT AATGGTCCTC AGGGCTCTAT TGGCTATCCT 
GGCCCTCGGG GATTGAAGGG AGCTCAAGGT ATCCGTGGAC TGAAAGGTCA TAAAGGAGAG 
AAGGGAGAGG ATGGATTCCC AGGAATTAAA GGAGACTTTG GTGTGAAGGG AGAGAGGGGA 
GAGGTGGGCG TGCCCGGCCC AAGAGGAGAA GATGGTCCTG AGGGGCCAAA GGGTCGCGTC 
GGACCCCCTG GTGAAATTGG TCCCCTCGGC CTGTTGGGAG AGAAGGGTAA ACTTGGTGTT 
CCTGGACTTC CTGGATATCC TGGACGTCAA GGAATAAAGG GGTCCCTTGG TTTCCCAGGA 
TTCCCTGGTA CAAATGGCGA GAAGGGCACA AGGGGTTTGA TTGGAAAACC TGGACCAAGA 
GGACAAAGAG GGCCGACAGG TCCTAGAGGG CAGAGAGGAC CACGTGGGGC AACTGGGAAA 
TCAGGTGCTA AGGGTGGATC AGGAAGTGAC GGGCCTCCTG GACCACCAGG AGAGAGAGGG 
CTAACTGGAC CTCAGGGTGC TAATGGATTC CCTGGGCCAA AGGGACCTCC CGGACCTCCA 
GGGAAAGATG GACTTCCTGG ACACCCTGGA CAGAGAGGCG AAGTCGGTTT CCAAGGCAAA 
GTTGGACCAC CTGGCCCACC TGGAGTTGTT GGCCCACACG GTCCCTCTGG TGAGACTGGT 
CAGATGGGTG AGCGTGGCCA CCCTGGACCT CCAGGACCAC CTGGTGAGCA AGGCCTTTCT 
GGCTCCTCTG GTAAAGAAGG AACCAAAGGA GATCCCGGTC CTCCTGGTGG CCCCGGTAAA 
GATGGACCCC CTGGACTTAG AGGATTCCCA GGAGAGAGAG GACTACCAGG AACTCCAGGT 
TCTGGAGGAC TGAAAGGAAA TGAAGGCCCT GCTGGACCAC CTGGTCCTGC GGGTTCATCT 
GGGGAGAGAG GTGGTGCTGG CACTGCAGGC CCTGTCGGCC CACCTGGACG ACCTGGACCT 
CAGGGACCCC CTGGAACTTC AGGTGAAAAG GGAGTACCAG GCGAGAAAGG TCCGGTTGGT 
CCAGCTGGTC GTGATGGCAT CCAAGGCCCA GTTGGTCTCC CAGGCCCTGC CGGACCTCCA 
GGTATTTCTG GAGAAGATGG AGACAAGGGT GAGGTTGGAG AGCCAGGACA AAAGGGAGCT 
AAAGGGTCCA AAGGAGAACA TGGTCCTCCT GGTCCACCTG GGCCGATGGG TCCTGTTGGA 
CAGCCTGGTG CAGCGGGTGC TGATGGTGAG ACGGGTCCAA GAGGTCAACA GGGACAGTTT 
GGTGCCAAGG GAGATGAAGG CACAAGAGGT TTCCCTGGTC CCCCTGGCCC CATAGGATTG 
CAGGGTTTGC CAGGCCCAGG TGGAGAAAAG GGAGAGACAG GAGATGTTGG ACCCTTGGGT 
CCTCCTGGTC CTCCAGGGCC GCGTGGTCCT GCTGGTCCTA ATGGTGCTGA TGGTCCCCAA 
GGTCCTCCAG GAGGTTTGGG TAATCCAGGT CCCATAGGAG AGAAGGGTGA GCCTGGTGAA 
TCTGGACCAC CTGGTGTTGG TGGAGAGCCA GGAAAATTGG GCCCAAGAGG AGAGCGTGGT 
GAGAAGGGAG AGTCCGGGCA GCCTGGCACC CCTGGTCCTC CTGGAGGAAA GGGACCCACT 
GGAGATGACG GACCCAAAGG AAACCCTGGT CCTGTTGGAT TCCCTGGTGA TCCTGGACCC 
CCTGGTGAAG TTGGACCACG AGGTCAAGAT GGTGCAAAGG GTGACAGAGG AGAGGATGGA 
GAACAAGGAG AAGCCGGCTC ACCTGGACCT ACTGGAGAGA ACGGACCACC TGGACCTCCA 
GGAAAGAGAG GTCCTGCAGG CACAAGGGGA CCGGAGGGCC GTCAAGGAGA GAAAGGAAGC 
AAGGGTGATT CAGGTGCTCT TGGTCCTCCC GGAAAAACTG GCCCTGTGGG GCCACAGGGA 
CAACCCGGAA AACCAGGAAC TGAGGGTCTA AGAGGGTTGC CTGGAACAGT GGGTGAGCAA 
GGTTCTCCAG GTGCTGCAGG CCCTAAAGGA CCTCCTGGAC CTCTGGGTCC TCCAGGTCTG 
GCTGGTCTTC GCGGTGATCC CGGTGCTAAA GGTGAGAAGG GACATCCAGG TATGCTCGGA 
CTGATCGGGC CTGCTGGAGA ACAAGGAGAG AAGGGAGACA GAGGCATGCC AGGACCACAG 
GGATCAACAG GACCCAAGGG AGAAACGGGT ATATCTGGAG GAACTGGTCC ACTTGGTCCT 
GCAGGTCCTG CTGGTATGCC TGGCCCGAGG GGTGTCAAAG GAGCTAAAGG TGCCAGTGGT 
GGGGCTGGTC CTAAAGGAGA AAAGGGTGTA CAAGGACCAC CAGGACCACC TGGTCCCCCT 
GGTGATGTCA TCCAGCCGCT GCCCATTCAG ATCCCCAAGA AGTCCAAGCG CTCCATCGAC 
GCCAGTAAGA TTCTGTCTGA GGCTGACGCT GAGGCTGCCG ACACCACTGG CTCTGAGTTC 
CTGACTGGTA ATGAAGGAAT GGAGGAGATC TTCGGCTCAC TTAATTCCCT GCGCCAGGAG 
ATCGAGAGCA TGCGCTTCCC ATTGGGCACA AAGGAGAGCC CCGGCCGCAC CTGCCAGGAT 
CTCCATCTCA GCCAACCAGA TCTGAAAGAC GGCGAGTATT GGATTGATCC AAATCAAGGC 
TGTGCTCGCG ACTCTTTCAA GGTTTACTGT AACTTCACTG CCGGCGGAGA GACCTGCCTG 
TATCCCAGCA AAGCAGTGGA GAATGTAAAG ATGAACTCTT GGATAAAGGA GAAGCCTGGG 
TCCTTGTTCA GTCAGTTTGC AAAGGGCAAC AAGTTTTCAT ATGTAGACTC GGTTGGAGAG 
GCTGTGGGTG TTGTTCAGCT GGGCTTCTTG CGGCTGCTCA GTGTCCAGGC CAGACAGAAC 
CTCACCTACC ACTGCCAACG CTCGGTGGCC TGGACTGACC AAACCACCGA CGGCGGATAC 
AAGCGGGCGC TGCGTCTGCA GGGGGCCAAT GATGAGGAGA TCAGCTATGA GACTAGCCCA 
TATATCAAAG CCCTCATCGA CGGCTGCTCC TATCGTAAGG GTCTGGACAG GACGGTGCTG 
GAGGTGAACA CACCTCAGGT GGAGCAGCTC CCTCTGCTGG ACATCAGGAT CTCAGACTTT 
GGGGAGGACA ATCAGAAATT TGGTTTTGAA GTAGGACCTG TCTGTTTCCT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159443.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159500.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.

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CloneID	ODa23889
Clone ID Related Accession (Same CDS sequence)	XM_005159443.3 , XM_005159443.2 , XM_005159442.3 , XM_005159442.4
Accession Version	XM_005159443.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGACGTAGA TCCTGCTTAC TCAGAGGCTG ACCACACCCC CACAGAGACC 
GATTATTATT ACACAGTAGA AGAAGAGGCG GCTCCACAGC CAGAGAGTGA TCTGACTGGA 
TATGACGAAA GCATTAACCA GGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159500.1
CDS	312..6377
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYAGKANNGKVTKQKTAPAAKPAPAKPAKSKPTPAPKTAKSKPTAAQLLISKIKPTPASKLAAKPKATPAPKNDKKKTNDNGKVNNGQTKVNGNGNGKSNGNGKDTNGKAKAGSNGKPNNNGNGNGKKTNGDAKINGNGPKTVKTVTKVASAKKSETPATKTVTGKPKAQVKAQPTKSKTDKTKANASPAKPKDTKAKAASVTQKPAVFKPTPVPLPRPSKTILDVNNVKSMSRNFPPFDKAVVSATTIKKKPTNGYQQDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159443.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159443.3

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGACGTAGA TCCTGCTTAC TCAGAGGCTG ACCACACCCC CACAGAGACC 
GATTATTATT ACACAGTAGA AGAAGAGGCG GCTCCACAGC CAGAGAGTGA TCTGACTGGA 
TATGACGAAA GCATTAACCA GGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159443.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159500.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.

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CloneID	ODa23889
Clone ID Related Accession (Same CDS sequence)	XM_005159443.3 , XM_005159443.2 , XM_005159442.3 , XM_005159442.4
Accession Version	XM_005159443.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(XI) chain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGACGTAGA TCCTGCTTAC TCAGAGGCTG ACCACACCCC CACAGAGACC 
GATTATTATT ACACAGTAGA AGAAGAGGCG GCTCCACAGC CAGAGAGTGA TCTGACTGGA 
TATGACGAAA GCATTAACCA GGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159500.1
CDS	317..6382
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYAGKANNGKVTKQKTAPAAKPAPAKPAKSKPTPAPKTAKSKPTAAQLLISKIKPTPASKLAAKPKATPAPKNDKKKTNDNGKVNNGQTKVNGNGNGKSNGNGKDTNGKAKAGSNGKPNNNGNGNGKKTNGDAKINGNGPKTVKTVTKVASAKKSETPATKTVTGKPKAQVKAQPTKSKTDKTKANASPAKPKDTKAKAASVTQKPAVFKPTPVPLPRPSKTILDVNNVKSMSRNFPPFDKAVVSATTIKKKPTNGYQQDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159443.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159443.2

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGACGTAGA TCCTGCTTAC TCAGAGGCTG ACCACACCCC CACAGAGACC 
GATTATTATT ACACAGTAGA AGAAGAGGCG GCTCCACAGC CAGAGAGTGA TCTGACTGGA 
TATGACGAAA GCATTAACCA GGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159442.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159499.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.

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CloneID	ODa23889
Clone ID Related Accession (Same CDS sequence)	XM_005159443.3 , XM_005159443.2 , XM_005159442.3 , XM_005159442.4
Accession Version	XM_005159442.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(XI) chain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGACGTAGA TCCTGCTTAC TCAGAGGCTG ACCACACCCC CACAGAGACC 
GATTATTATT ACACAGTAGA AGAAGAGGCG GCTCCACAGC CAGAGAGTGA TCTGACTGGA 
TATGACGAAA GCATTAACCA GGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159499.1
CDS	384..6449
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYAGKANNGKVTKQKTAPAAKPAPAKPAKSKPTPAPKTAKSKPTAAQLLISKIKPTPASKLAAKPKATPAPKNDKKKTNDNGKVNNGQTKVNGNGNGKSNGNGKDTNGKAKAGSNGKPNNNGNGNGKKTNGDAKINGNGPKTVKTVTKVASAKKSETPATKTVTGKPKAQVKAQPTKSKTDKTKANASPAKPKDTKAKAASVTQKPAVFKPTPVPLPRPSKTILDVNNVKSMSRNFPPFDKAVVSATTIKKKPTNGYQQDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159442.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159442.3

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGACGTAGA TCCTGCTTAC TCAGAGGCTG ACCACACCCC CACAGAGACC 
GATTATTATT ACACAGTAGA AGAAGAGGCG GCTCCACAGC CAGAGAGTGA TCTGACTGGA 
TATGACGAAA GCATTAACCA GGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a2 ( XM_005159442.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159499.1 Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.

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CloneID	ODa23889
Clone ID Related Accession (Same CDS sequence)	XM_005159443.3 , XM_005159443.2 , XM_005159442.3 , XM_005159442.4
Accession Version	XM_005159442.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(XI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159442.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGACGTAGA TCCTGCTTAC TCAGAGGCTG ACCACACCCC CACAGAGACC 
GATTATTATT ACACAGTAGA AGAAGAGGCG GCTCCACAGC CAGAGAGTGA TCTGACTGGA 
TATGACGAAA GCATTAACCA GGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159499.1
CDS	396..6461
Translation	MDIRKKRRPWCMETSFVLLLCLSMAQADPVDVLRVLQLPSLPEGVQKVPGFCTSRLSGTPDHAYRITKKAQISAPTKQLFSGRFPENFSIMTLVKAKAGLQAFLLSIYNEQGVQQLGLELGRSPIFLYEDQHRKPAPEDYPLFKGVNLADGKWHRIAISVQKKNITLILDCKNRITKTLPRSNNPVLDTKGITVFGARLLDEEVFQGEIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQSQDPNTYAGKANNGKVTKQKTAPAAKPAPAKPAKSKPTPAPKTAKSKPTAAQLLISKIKPTPASKLAAKPKATPAPKNDKKKTNDNGKVNNGQTKVNGNGNGKSNGNGKDTNGKAKAGSNGKPNNNGNGNGKKTNGDAKINGNGPKTVKTVTKVASAKKSETPATKTVTGKPKAQVKAQPTKSKTDKTKANASPAKPKDTKAKAASVTQKPAVFKPTPVPLPRPSKTILDVNNVKSMSRNFPPFDKAVVSATTIKKKPTNGYQQDVDPAYSEADHTPTETDYYYTVEEEAAPQPESDLTGYDESINQVDETVPVEEMDATKTGGEVASQTPEEPFTEEYITSDFGMKEYDYSYKDYNEPIIESKTEDTLGPALSAVTEEGGASVRAIKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPQGQPGSVGDPGERGPTGKAGLPGADGVPGPPGTSVMLPFRFGQSAGEKGPVASAQEAQAQAILSQARLALKGPSGPMGFTGRPGPLGTPGSPGFKGERGDPGAQGPRGPQGVSGPPGKAGRRGRAGADGARGMPGESGIKGDRGFDGLPGLPGDKGYRGQPGGMGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPSGLPGPPGVRGNDGPHGPKGNLGPQGEPGPPGQQGAPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPGGTKGNQGPNGPQGSIGYPGPRGLKGAQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGPLGLLGEKGKLGVPGLPGYPGRQGIKGSLGFPGFPGTNGEKGTRGLIGKPGPRGQRGPTGPRGQRGPRGATGKSGAKGGSGSDGPPGPPGERGLTGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKVGPPGPPGVVGPHGPSGETGQMGERGHPGPPGPPGEQGLSGSSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGSGGLKGNEGPAGPPGPAGSSGERGGAGTAGPVGPPGRPGPQGPPGTSGEKGVPGEKGPVGPAGRDGIQGPVGLPGPAGPPGISGEDGDKGEVGEPGQKGAKGSKGEHGPPGPPGPMGPVGQPGAAGADGETGPRGQQGQFGAKGDEGTRGFPGPPGPIGLQGLPGPGGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGVGGEPGKLGPRGERGEKGESGQPGTPGPPGGKGPTGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGDRGEDGEQGEAGSPGPTGENGPPGPPGKRGPAGTRGPEGRQGEKGSKGDSGALGPPGKTGPVGPQGQPGKPGTEGLRGLPGTVGEQGSPGAAGPKGPPGPLGPPGLAGLRGDPGAKGEKGHPGMLGLIGPAGEQGEKGDRGMPGPQGSTGPKGETGISGGTGPLGPAGPAGMPGPRGVKGAKGASGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASKILSEADAEAADTTGSEFLTGNEGMEEIFGSLNSLRQEIESMRFPLGTKESPGRTCQDLHLSQPDLKDGEYWIDPNQGCARDSFKVYCNFTAGGETCLYPSKAVENVKMNSWIKEKPGSLFSQFAKGNKFSYVDSVGEAVGVVQLGFLRLLSVQARQNLTYHCQRSVAWTDQTTDGGYKRALRLQGANDEEISYETSPYIKALIDGCSYRKGLDRTVLEVNTPQVEQLPLLDIRISDFGEDNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005159442.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159442.4

ORF Insert Sequence:

ATGGATATTC GGAAGAAGCG GAGGCCGTGG TGCATGGAGA CCTCGTTTGT GCTCCTCCTG 
TGCCTTTCTA TGGCACAAGC AGATCCAGTA GATGTTTTGC GGGTGCTGCA GTTGCCTTCC 
CTTCCTGAAG GTGTGCAGAA GGTTCCGGGT TTCTGTACCT CCCGATTGTC TGGGACTCCT 
GATCATGCCT ACCGCATCAC TAAAAAAGCC CAGATCTCTG CCCCCACCAA ACAGCTATTC 
TCAGGACGTT TCCCGGAGAA CTTCTCCATC ATGACTCTGG TAAAGGCCAA GGCTGGTCTT 
CAAGCCTTCC TGTTGTCCAT CTATAATGAG CAGGGTGTGC AGCAGCTTGG GCTGGAGCTG 
GGACGCTCAC CCATCTTCCT GTATGAGGAC CAGCATCGCA AACCTGCCCC AGAGGACTAC 
CCACTGTTCA AAGGCGTCAA CCTGGCTGAC GGCAAGTGGC ACCGCATTGC CATTTCTGTG 
CAGAAGAAGA ACATCACTCT GATCCTGGAC TGCAAGAATC GCATCACCAA AACTCTGCCA 
AGGAGCAACA ACCCAGTGTT GGACACCAAG GGCATCACTG TTTTTGGAGC CCGTCTCCTG 
GATGAGGAGG TTTTCCAGGG TGAGATCCAG CAGCTGCTGA TCGCATCCAA CCCTCAGGCC 
GCATATGACT TCTGTGAGCA TTACAGCCCT GACTGCGACT CTCCTCTGCC CAAAGTCCAG 
TCCCAGGACC CAAACACCTA TGCGGGCAAG GCAAACAATG GCAAAGTCAC TAAGCAAAAA 
ACTGCTCCAG CTGCAAAGCC TGCTCCAGCT AAACCAGCTA AATCCAAGCC AACACCAGCA 
CCCAAAACAG CAAAGAGCAA ACCTACAGCA GCACAATTAT TGATTTCAAA GATAAAACCT 
ACTCCTGCTT CTAAACTAGC AGCTAAACCA AAAGCAACTC CAGCACCAAA GAATGACAAA 
AAGAAGACCA ACGACAATGG AAAGGTCAAT AATGGTCAAA CTAAAGTAAA TGGAAATGGT 
AATGGCAAGA GCAATGGAAA TGGTAAAGAC ACTAATGGGA AGGCTAAGGC TGGCAGCAAT 
GGTAAACCCA ATAACAACGG CAACGGCAAT GGAAAAAAAA CAAACGGAGA TGCTAAAATT 
AATGGCAATG GCCCGAAAAC AGTAAAGACT GTGACCAAAG TAGCATCAGC CAAGAAGTCA 
GAAACACCCG CTACGAAAAC TGTAACAGGA AAACCTAAAG CGCAGGTCAA AGCACAACCA 
ACCAAAAGCA AGACAGACAA AACGAAAGCC AATGCGTCCC CCGCAAAACC AAAAGACACC 
AAAGCCAAAG CAGCCTCCGT CACTCAAAAA CCTGCTGTTT TCAAACCCAC CCCCGTGCCT 
TTGCCTCGAC CTTCAAAAAC CATCCTCGAT GTCAATAACG TCAAATCAAT GTCCAGAAAT 
TTCCCACCCT TCGACAAGGC TGTTGTGAGT GCCACCACCA TCAAGAAGAA ACCGACCAAT 
GGTTACCAAC AGGACGTAGA TCCTGCTTAC TCAGAGGCTG ACCACACCCC CACAGAGACC 
GATTATTATT ACACAGTAGA AGAAGAGGCG GCTCCACAGC CAGAGAGTGA TCTGACTGGA 
TATGACGAAA GCATTAACCA GGTCGACGAG ACTGTCCCCG TTGAGGAAAT GGATGCTACC 
AAGACAGGTG GTGAGGTGGC GTCTCAAACG CCAGAAGAGC CCTTCACTGA GGAGTACATT 
ACAAGCGACT TTGGGATGAA GGAGTATGAC TATTCCTACA AGGACTACAA TGAGCCCATC 
ATTGAGAGCA AGACGGAGGA CACTCTAGGC CCTGCCCTGT CCGCTGTGAC AGAGGAGGGG 
GGTGCCTCAG TGCGAGCCAT TAAGGGAGAG AAGGGAGAGC CTGCTGTCCT TGAACCTGGC 
ATGCTGATTG AAGGACCACC AGGACCAGAA GGACCAGCAG GTCTCCCTGG CCCCTCTGGC 
CCCCAAGGAC AACCTGGTTC TGTTGGTGAT CCTGGTGAGA GGGGTCCTAC TGGTAAGGCT 
GGTCTACCTG GAGCAGATGG TGTTCCAGGA CCTCCAGGAA CCTCTGTCAT GCTTCCTTTC 
CGCTTTGGAC AGAGCGCTGG AGAGAAAGGA CCTGTTGCCT CTGCACAGGA AGCCCAAGCT 
CAAGCCATTC TGTCTCAAGC TCGGCTTGCC CTGAAAGGAC CATCCGGGCC AATGGGTTTC 
ACTGGCCGTC CTGGACCTTT GGGAACACCT GGAAGTCCTG GCTTTAAGGG AGAAAGGGGG 
GACCCTGGTG CTCAGGGTCC TAGAGGTCCT CAGGGTGTGT CTGGTCCTCC TGGCAAAGCT 
GGTCGGAGGG GTCGCGCTGG TGCTGATGGA GCCAGAGGGA TGCCAGGAGA GTCTGGAATT 
AAGGGTGACC GTGGTTTTGA TGGCCTTCCT GGTTTGCCTG GTGACAAGGG TTATCGGGGT 
CAACCTGGAG GAATGGGACC CCCAGGACCA CATGGAGAGG ACGGAGAGAG GGGAGATGAT 
GGAGATGTTG GACCCAGAGG TCTTCCTGGC GAACCTGGAC CCCGTGGTTT GTTGGGGCCT 
AAAGGGCCTT CTGGACTTCC TGGACCTCCT GGTGTTCGTG GAAATGATGG TCCACATGGT 
CCAAAAGGAA ACTTAGGACC ACAAGGAGAG CCCGGTCCTC CAGGCCAGCA GGGGGCTCCA 
GGAACACAGG GAATGCCAGG TCCACAGGGT GCCACTGGAC CACCAGGAGA GAAAGGACCC 
ACAGGAAAGC CAGGTCTACC AGGAATGCCA GGAGCTGATG GTCCTCCCGG TCATCCCGGG 
AAGGAAGGTC CTGGTGGAAC CAAAGGAAAT CAAGGTCCTA ATGGTCCTCA GGGCTCTATT 
GGCTATCCTG GCCCTCGGGG ATTGAAGGGA GCTCAAGGTA TCCGTGGACT GAAAGGTCAT 
AAAGGAGAGA AGGGAGAGGA TGGATTCCCA GGAATTAAAG GAGACTTTGG TGTGAAGGGA 
GAGAGGGGAG AGGTGGGCGT GCCCGGCCCA AGAGGAGAAG ATGGTCCTGA GGGGCCAAAG 
GGTCGCGTCG GACCCCCTGG TGAAATTGGT CCCCTCGGCC TGTTGGGAGA GAAGGGTAAA 
CTTGGTGTTC CTGGACTTCC TGGATATCCT GGACGTCAAG GAATAAAGGG GTCCCTTGGT 
TTCCCAGGAT TCCCTGGTAC AAATGGCGAG AAGGGCACAA GGGGTTTGAT TGGAAAACCT 
GGACCAAGAG GACAAAGAGG GCCGACAGGT CCTAGAGGGC AGAGAGGACC ACGTGGGGCA 
ACTGGGAAAT CAGGTGCTAA GGGTGGATCA GGAAGTGACG GGCCTCCTGG ACCACCAGGA 
GAGAGAGGGC TAACTGGACC TCAGGGTGCT AATGGATTCC CTGGGCCAAA GGGACCTCCC 
GGACCTCCAG GGAAAGATGG ACTTCCTGGA CACCCTGGAC AGAGAGGCGA AGTCGGTTTC 
CAAGGCAAAG TTGGACCACC TGGCCCACCT GGAGTTGTTG GCCCACACGG TCCCTCTGGT 
GAGACTGGTC AGATGGGTGA GCGTGGCCAC CCTGGACCTC CAGGACCACC TGGTGAGCAA 
GGCCTTTCTG GCTCCTCTGG TAAAGAAGGA ACCAAAGGAG ATCCCGGTCC TCCTGGTGGC 
CCCGGTAAAG ATGGACCCCC TGGACTTAGA GGATTCCCAG GAGAGAGAGG ACTACCAGGA 
ACTCCAGGTT CTGGAGGACT GAAAGGAAAT GAAGGCCCTG CTGGACCACC TGGTCCTGCG 
GGTTCATCTG GGGAGAGAGG TGGTGCTGGC ACTGCAGGCC CTGTCGGCCC ACCTGGACGA 
CCTGGACCTC AGGGACCCCC TGGAACTTCA GGTGAAAAGG GAGTACCAGG CGAGAAAGGT 
CCGGTTGGTC CAGCTGGTCG TGATGGCATC CAAGGCCCAG TTGGTCTCCC AGGCCCTGCC 
GGACCTCCAG GTATTTCTGG AGAAGATGGA GACAAGGGTG AGGTTGGAGA GCCAGGACAA 
AAGGGAGCTA AAGGGTCCAA AGGAGAACAT GGTCCTCCTG GTCCACCTGG GCCGATGGGT 
CCTGTTGGAC AGCCTGGTGC AGCGGGTGCT GATGGTGAGA CGGGTCCAAG AGGTCAACAG 
GGACAGTTTG GTGCCAAGGG AGATGAAGGC ACAAGAGGTT TCCCTGGTCC CCCTGGCCCC 
ATAGGATTGC AGGGTTTGCC AGGCCCAGGT GGAGAAAAGG GAGAGACAGG AGATGTTGGA 
CCCTTGGGTC CTCCTGGTCC TCCAGGGCCG CGTGGTCCTG CTGGTCCTAA TGGTGCTGAT 
GGTCCCCAAG GTCCTCCAGG AGGTTTGGGT AATCCAGGTC CCATAGGAGA GAAGGGTGAG 
CCTGGTGAAT CTGGACCACC TGGTGTTGGT GGAGAGCCAG GAAAATTGGG CCCAAGAGGA 
GAGCGTGGTG AGAAGGGAGA GTCCGGGCAG CCTGGCACCC CTGGTCCTCC TGGAGGAAAG 
GGACCCACTG GAGATGACGG ACCCAAAGGA AACCCTGGTC CTGTTGGATT CCCTGGTGAT 
CCTGGACCCC CTGGTGAAGT TGGACCACGA GGTCAAGATG GTGCAAAGGG TGACAGAGGA 
GAGGATGGAG AACAAGGAGA AGCCGGCTCA CCTGGACCTA CTGGAGAGAA CGGACCACCT 
GGACCTCCAG GAAAGAGAGG TCCTGCAGGC ACAAGGGGAC CGGAGGGCCG TCAAGGAGAG 
AAAGGAAGCA AGGGTGATTC AGGTGCTCTT GGTCCTCCCG GAAAAACTGG CCCTGTGGGG 
CCACAGGGAC AACCCGGAAA ACCAGGAACT GAGGGTCTAA GAGGGTTGCC TGGAACAGTG 
GGTGAGCAAG GTTCTCCAGG TGCTGCAGGC CCTAAAGGAC CTCCTGGACC TCTGGGTCCT 
CCAGGTCTGG CTGGTCTTCG CGGTGATCCC GGTGCTAAAG GTGAGAAGGG ACATCCAGGT 
ATGCTCGGAC TGATCGGGCC TGCTGGAGAA CAAGGAGAGA AGGGAGACAG AGGCATGCCA 
GGACCACAGG GATCAACAGG ACCCAAGGGA GAAACGGGTA TATCTGGAGG AACTGGTCCA 
CTTGGTCCTG CAGGTCCTGC TGGTATGCCT GGCCCGAGGG GTGTCAAAGG AGCTAAAGGT 
GCCAGTGGTG GGGCTGGTCC TAAAGGAGAA AAGGGTGTAC AAGGACCACC AGGACCACCT 
GGTCCCCCTG GTGATGTCAT CCAGCCGCTG CCCATTCAGA TCCCCAAGAA GTCCAAGCGC 
TCCATCGACG CCAGTAAGAT TCTGTCTGAG GCTGACGCTG AGGCTGCCGA CACCACTGGC 
TCTGAGTTCC TGACTGGTAA TGAAGGAATG GAGGAGATCT TCGGCTCACT TAATTCCCTG 
CGCCAGGAGA TCGAGAGCAT GCGCTTCCCA TTGGGCACAA AGGAGAGCCC CGGCCGCACC 
TGCCAGGATC TCCATCTCAG CCAACCAGAT CTGAAAGACG GCGAGTATTG GATTGATCCA 
AATCAAGGCT GTGCTCGCGA CTCTTTCAAG GTTTACTGTA ACTTCACTGC CGGCGGAGAG 
ACCTGCCTGT ATCCCAGCAA AGCAGTGGAG AATGTAAAGA TGAACTCTTG GATAAAGGAG 
AAGCCTGGGT CCTTGTTCAG TCAGTTTGCA AAGGGCAACA AGTTTTCATA TGTAGACTCG 
GTTGGAGAGG CTGTGGGTGT TGTTCAGCTG GGCTTCTTGC GGCTGCTCAG TGTCCAGGCC 
AGACAGAACC TCACCTACCA CTGCCAACGC TCGGTGGCCT GGACTGACCA AACCACCGAC 
GGCGGATACA AGCGGGCGCT GCGTCTGCAG GGGGCCAATG ATGAGGAGAT CAGCTATGAG 
ACTAGCCCAT ATATCAAAGC CCTCATCGAC GGCTGCTCCT ATCGTAAGGG TCTGGACAGG 
ACGGTGCTGG AGGTGAACAC ACCTCAGGTG GAGCAGCTCC CTCTGCTGGA CATCAGGATC 
TCAGACTTTG GGGAGGACAA TCAGAAATTT GGTTTTGAAG TAGGACCTGT CTGTTTCCTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	col11a2
Entrez Gene ID	561679
Full Name	collagen, type XI, alpha 2
Synonyms	col11a1
General protein information	Preferred Names collagen, type XI, alpha 2 Names collagen alpha-2(XI) chain collagen XI alpha 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Canis lupus familiaris (dog)	COL11A2	XP_005627252.1
Mus musculus (house mouse)	Col11a2	NP_034056.1
Homo sapiens (human)	COL11A2	NP_542411.2
Danio rerio (zebrafish)	col11a2	NP_001073461.1
Bos taurus (cattle)	COL11A2	NP_001039664.1
Rattus norvegicus (Norway rat)	Col11a2	NP_997693.1
	COL11A2	XP_002808428.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

col11a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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