ide cDNA ORF clone, Danio rerio(zebrafish)

The following ide gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ide cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa31317 NM_001089525.1
Latest version!
Danio rerio insulin-degrading enzyme (ide), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa19075 XM_005169527.4
Latest version!
Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa19076 XM_005169528.4
Latest version!
Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
ODa19076 XM_005169528.3 Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa19075 XM_005169527.3 Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa31317
    Clone ID Related Accession (Same CDS sequence) NM_001089525.1
    Accession Version NM_001089525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Danio rerio(zebrafish)
    Product insulin-degrading enzyme
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139607.1. On Nov 18, 2007 this sequence version replaced XM_679644.2. ##Evidence-Data-START## Transcript exon combination :: BC139607.1, AB303424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC GTCAATGGTG GTGAAGCTTT TTGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCTGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTCATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG
    TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG
    GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA
    AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GATGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GGTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TTTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCC
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001082994.1
    CDS144..3080
    Translation

    Target ORF information:

    RefSeq Version NM_001089525.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio insulin-degrading enzyme (ide), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001089525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC GTCAATGGTG GTGAAGCTTT TTGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCTGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTCATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG
    TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG
    GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA
    AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GATGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GGTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TTTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCC
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa19075
    Clone ID Related Accession (Same CDS sequence) XM_005169527.3 , XM_005169527.4
    Accession Version XM_005169527.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product insulin-degrading enzyme isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169527.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG
    TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG
    GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA
    AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169584.1
    CDS333..3329
    Translation

    Target ORF information:

    RefSeq Version XM_005169527.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169527.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG
    TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG
    GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA
    AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa19075
    Clone ID Related Accession (Same CDS sequence) XM_005169527.3 , XM_005169527.4
    Accession Version XM_005169527.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product insulin-degrading enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169527.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG
    TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG
    GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA
    AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169584.1
    CDS333..3329
    Translation

    Target ORF information:

    RefSeq Version XM_005169527.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169527.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG
    TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG
    GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA
    AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa19076
    Clone ID Related Accession (Same CDS sequence) XM_005169528.3 , XM_005169528.4
    Accession Version XM_005169528.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product insulin-degrading enzyme isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGTCGC
    TACCTTTATG CAGACCCAGT GCACTGCAAC ATGACTTACC TGTTTCTCAG GTTGTTGAAG
    GATGATTTGA GAGAGTATAC ATATGCAGCC CGCCTGGCAG GGTTGGTCTA TGGCATAGCC
    TCAGGAATGA ATGCCATCCT TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169585.1
    CDS333..3329
    Translation

    Target ORF information:

    RefSeq Version XM_005169528.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169528.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGTCGC
    TACCTTTATG CAGACCCAGT GCACTGCAAC ATGACTTACC TGTTTCTCAG GTTGTTGAAG
    GATGATTTGA GAGAGTATAC ATATGCAGCC CGCCTGGCAG GGTTGGTCTA TGGCATAGCC
    TCAGGAATGA ATGCCATCCT TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa19076
    Clone ID Related Accession (Same CDS sequence) XM_005169528.3 , XM_005169528.4
    Accession Version XM_005169528.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product insulin-degrading enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169528.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGTCGC
    TACCTTTATG CAGACCCAGT GCACTGCAAC ATGACTTACC TGTTTCTCAG GTTGTTGAAG
    GATGATTTGA GAGAGTATAC ATATGCAGCC CGCCTGGCAG GGTTGGTCTA TGGCATAGCC
    TCAGGAATGA ATGCCATCCT TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169585.1
    CDS333..3329
    Translation

    Target ORF information:

    RefSeq Version XM_005169528.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169528.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
    ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA
    CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC
    ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG
    AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT
    CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC
    ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG
    TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA
    GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG
    TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC
    AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG
    CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA
    GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG
    AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC
    TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC
    CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG
    GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA
    GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA
    GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG
    CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT
    TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG
    CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA
    GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC
    TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA
    CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG
    TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG
    AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC
    AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGTCGC
    TACCTTTATG CAGACCCAGT GCACTGCAAC ATGACTTACC TGTTTCTCAG GTTGTTGAAG
    GATGATTTGA GAGAGTATAC ATATGCAGCC CGCCTGGCAG GGTTGGTCTA TGGCATAGCC
    TCAGGAATGA ATGCCATCCT TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG
    CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC
    ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC
    GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA
    GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA
    CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG
    ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT
    CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG
    AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC
    ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC
    ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT
    GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT
    CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC
    TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG
    GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT
    AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG
    GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA
    GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT
    CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT
    CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.