mepce cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mepce
Entrez Gene ID	561384
Full Name	methylphosphate capping enzyme
Synonyms	fc59f06,si:ch211-283h6.1,wu:fc59f06,zBCDIN3
General protein information	Preferred Names methylphosphate capping enzyme Names 7SK snRNA methylphosphate capping enzyme bcdin3 bicoid-interacting protein 3 homolog bin3 homolog bin3, bicoid-interacting 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128529.1	NP_001122001.1	7SK snRNA methylphosphate capping enzyme
XM_021476212.1	XP_021331887.1	7SK snRNA methylphosphate capping enzyme isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC100910540	XP_003751202.1
Danio rerio (zebrafish)	si:ch1073-157b13.1	XP_001921729.2
Danio rerio (zebrafish)	mepce	NP_001122001.1
Rattus norvegicus (Norway rat)	Mepce	NP_001094148.1
Schizosaccharomyces pombe (fission yeast)	SPBC2A9.10	NP_596220.1
Xenopus tropicalis (tropical clawed frog)	mepce	XP_002936507.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.
Barboric M, Lenasi T, Chen H, Johansen EB, Guo S, Peterlin BM
Proceedings of the National Academy of Sciences of the United States of America106(19)7798-803(2009 May)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary.
Zeng S, Gong Z
Gene294(1-2)45-53(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mepce gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mepce cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00154	NM_001128529.1 Latest version!	Danio rerio methylphosphate capping enzyme (mepce), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa00154	XM_021476212.1 Latest version!	Danio rerio methylphosphate capping enzyme (mepce), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mepce ( NM_001128529.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001122001.1 Danio rerio methylphosphate capping enzyme (mepce), mRNA.

Price & Availability↑

CloneID	ODa00154
Clone ID Related Accession (Same CDS sequence)	NM_001128529.1 , XM_021476212.1
Accession Version	NM_001128529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Danio rerio(zebrafish)
Product	7SK snRNA methylphosphate capping enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX537304.14. On Jul 31, 2008 this sequence version replaced XM_684795.3. ##Evidence-Data-START## Transcript exon combination :: GFIL01026229.1, GDQH01009879.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATAGAGA TGTCATTTGA CAAAGAAACT GTTTTATCTT GTGAGGGGCA AACAGACCCC 
ACTTCTCAAG AATTTAATAT AAATGATACC AGTGCTCTCC ATCCACCCGG GAACACCACA 
GAGAACAACC TCAGTAAGGA CGCAGGAAGT GAGGCATCCC TAGAGAAATC TGCTACCACC 
GATAACAATG CTTCAAGTGA AAGTGCAGTT CTTGGGGCTG AATCGTCTAA ACAATCGCAC 
AATGGCACCA AACCACAACA GCAGAAAGTC AACAAGCGCC GCAACACTAT GAGCGCTGGG 
TTTAAGCACC CGGGTTATGG CAAACGACGA CGGCGAGCCA ACTCGGAAAG CGAGTCAGTC 
CTGCCAACCA ATTTCCTGCT GGGCGGGAAC ATTTTTGACC CACTCAACCT TAACAGCCTT 
TTAGACGAAG AGGTCAACAA GGCTCTCAAT GCAGAGACGC CCAAGTCTTC GCCATTGCCA 
GCCAAAAACC GAGACCCTGT TGAGATCTTG ATACCCAGAG ATATCACAGA CCCCCTCAAC 
CTCAACTGCC TGAGCACAGG AGACAGTCTG CTGGTGTCTC CATTGAAGAG TGGTGGAGGA 
AGGCGGAGAC ATCGAAACAA ACACCATGGA GCAAACACTG GACGAGTTTC CACTGAAGGA 
ATTGGTTTAA CGGAGTCTAA TGGCTGTGCA GCAGTGAAGG ATGATGGAAC ATCATTGGTC 
TCGATGACTA ATGTGCATTT AGAAAGTACA ATTGCGGATC AAACTCTGTT GGACTCGACT 
CTCGAGTCTT CAAATAAGTC CAGTCTAACT TCAAATGACA ATGAGGTGAA GGAGAAAACC 
GTTGACTGCT CAACCGCACA TCTAACAGCA AACCCAACCA ACCCTCCATC AAGCAGGCAG 
CGCAAACGCA GGCGAGCCGG CAGCAGAACG GAGAACTCCA GTTCTTCTTC TGAGGTGAAA 
AGATCGGGGA GACATTTTCA ACACAATGAA TCCCAAGGAG GAAACTCTCG AAGTGGAGTC 
TCTGATAGGC AGCAACCGTA TCCGAATCAA TGGAGGCCGC AGTACAGCAA CAACAATCAG 
AACCCGCAGC AGCCCAAAAA GAAGTTTCAA TACGGTAACT ATAACAAATA CTACGGCTAT 
CGGAACCCAG GGATGAGCGA GGATCCTCGC ATTCGTGTTA TGAATCCGGA TTGGTTCAGA 
GGGAAGGATG TGTTGGATCT GGGATGCAAC ACTGGCCACC TGACTCTGTT TATCGCAAAA 
AATTGGCGGC CTGCTAGTAT TGTGGGCCTG GATATTGACG GATCGCTGAT ACATGCTGCT 
CGACAGAATA TAAGACACTA CCTCTCTGAG GTCCAAGTGC AGCACTCCCG ACGCTCTGGG 
GAAAATACAA AGGCAGATCG AGGGGAGGTT TCGGGAGAAG AAAAGGACAA AGATAAAACT 
AGTTTGGTTA TGGATGAAAA AAAGGCAAAG CATGTTGACG AAGTGAATAA ATGCATGGAC 
GAAGGAATGG AAGTTAATCA AGAAGAGAAG AGGGAGGCTG ATCGGGGAGA AATAGGGGAT 
GGAGCATCGG TAGATCTCCC TGATGGGAAA CATTCGTTCC CCGTCTCGCT CCGGATCTCA 
AGAGGACCCA TTGCTGGGCC CCCTTTGCCC GAGACAAACA CACACAGTCT CCCTCCTGGA 
GATTTCCCTG CCAACGTGAC CTTTATTAAG GGGAACTATG TGCTGGAGAG TGATGTGCTG 
CTGCAGACAC AGAGGGAGGA GTACGATGTC ATCCTGTGTT TGAGTGTGAC AAAGTGGGTC 
CACTTGAACT GGGGCGATGC AGGACTCAAG CGCTTCTTTC ACAGGGTATA CAAGCACCTG 
CGGCCAGGGG GACTCTTCAT TCTGGAGCCC CAGCCCTGGA GCTCCTACAA CAAGAGGAAG 
AAGCTCACCG AGGCCATCTG TAAGAATTAC CACAGCATCC GGTTAAAACC AGACCAGTTC 
TCCTCTTTCC TGACGACTGA GGTGGGCTTC TCCAGTTATG AGCTCATTGG GACGTCGCAA 
AACTACTCCA AAGGTTTTCA GAGACCAATC TCTCTGTATC ACAAACGACC TTCATCTTTA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122001.1
CDS	562..2667
Translation	MIEMSFDKETVLSCEGQTDPTSQEFNINDTSALHPPGNTTENNLSKDAGSEASLEKSATTDNNASSESAVLGAESSKQSHNGTKPQQQKVNKRRNTMSAGFKHPGYGKRRRRANSESESVLPTNFLLGGNIFDPLNLNSLLDEEVNKALNAETPKSSPLPAKNRDPVEILIPRDITDPLNLNCLSTGDSLLVSPLKSGGGRRRHRNKHHGANTGRVSTEGIGLTESNGCAAVKDDGTSLVSMTNVHLESTIADQTLLDSTLESSNKSSLTSNDNEVKEKTVDCSTAHLTANPTNPPSSRQRKRRRAGSRTENSSSSSEVKRSGRHFQHNESQGGNSRSGVSDRQQPYPNQWRPQYSNNNQNPQQPKKKFQYGNYNKYYGYRNPGMSEDPRIRVMNPDWFRGKDVLDLGCNTGHLTLFIAKNWRPASIVGLDIDGSLIHAARQNIRHYLSEVQVQHSRRSGENTKADRGEVSGEEKDKDKTSLVMDEKKAKHVDEVNKCMDEGMEVNQEEKREADRGEIGDGASVDLPDGKHSFPVSLRISRGPIAGPPLPETNTHSLPPGDFPANVTFIKGNYVLESDVLLQTQREEYDVILCLSVTKWVHLNWGDAGLKRFFHRVYKHLRPGGLFILEPQPWSSYNKRKKLTEAICKNYHSIRLKPDQFSSFLTTEVGFSSYELIGTSQNYSKGFQRPISLYHKRPSSLK

Target ORF information:

RefSeq Version	NM_001128529.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylphosphate capping enzyme (mepce), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128529.1

ORF Insert Sequence:

ATGATAGAGA TGTCATTTGA CAAAGAAACT GTTTTATCTT GTGAGGGGCA AACAGACCCC 
ACTTCTCAAG AATTTAATAT AAATGATACC AGTGCTCTCC ATCCACCCGG GAACACCACA 
GAGAACAACC TCAGTAAGGA CGCAGGAAGT GAGGCATCCC TAGAGAAATC TGCTACCACC 
GATAACAATG CTTCAAGTGA AAGTGCAGTT CTTGGGGCTG AATCGTCTAA ACAATCGCAC 
AATGGCACCA AACCACAACA GCAGAAAGTC AACAAGCGCC GCAACACTAT GAGCGCTGGG 
TTTAAGCACC CGGGTTATGG CAAACGACGA CGGCGAGCCA ACTCGGAAAG CGAGTCAGTC 
CTGCCAACCA ATTTCCTGCT GGGCGGGAAC ATTTTTGACC CACTCAACCT TAACAGCCTT 
TTAGACGAAG AGGTCAACAA GGCTCTCAAT GCAGAGACGC CCAAGTCTTC GCCATTGCCA 
GCCAAAAACC GAGACCCTGT TGAGATCTTG ATACCCAGAG ATATCACAGA CCCCCTCAAC 
CTCAACTGCC TGAGCACAGG AGACAGTCTG CTGGTGTCTC CATTGAAGAG TGGTGGAGGA 
AGGCGGAGAC ATCGAAACAA ACACCATGGA GCAAACACTG GACGAGTTTC CACTGAAGGA 
ATTGGTTTAA CGGAGTCTAA TGGCTGTGCA GCAGTGAAGG ATGATGGAAC ATCATTGGTC 
TCGATGACTA ATGTGCATTT AGAAAGTACA ATTGCGGATC AAACTCTGTT GGACTCGACT 
CTCGAGTCTT CAAATAAGTC CAGTCTAACT TCAAATGACA ATGAGGTGAA GGAGAAAACC 
GTTGACTGCT CAACCGCACA TCTAACAGCA AACCCAACCA ACCCTCCATC AAGCAGGCAG 
CGCAAACGCA GGCGAGCCGG CAGCAGAACG GAGAACTCCA GTTCTTCTTC TGAGGTGAAA 
AGATCGGGGA GACATTTTCA ACACAATGAA TCCCAAGGAG GAAACTCTCG AAGTGGAGTC 
TCTGATAGGC AGCAACCGTA TCCGAATCAA TGGAGGCCGC AGTACAGCAA CAACAATCAG 
AACCCGCAGC AGCCCAAAAA GAAGTTTCAA TACGGTAACT ATAACAAATA CTACGGCTAT 
CGGAACCCAG GGATGAGCGA GGATCCTCGC ATTCGTGTTA TGAATCCGGA TTGGTTCAGA 
GGGAAGGATG TGTTGGATCT GGGATGCAAC ACTGGCCACC TGACTCTGTT TATCGCAAAA 
AATTGGCGGC CTGCTAGTAT TGTGGGCCTG GATATTGACG GATCGCTGAT ACATGCTGCT 
CGACAGAATA TAAGACACTA CCTCTCTGAG GTCCAAGTGC AGCACTCCCG ACGCTCTGGG 
GAAAATACAA AGGCAGATCG AGGGGAGGTT TCGGGAGAAG AAAAGGACAA AGATAAAACT 
AGTTTGGTTA TGGATGAAAA AAAGGCAAAG CATGTTGACG AAGTGAATAA ATGCATGGAC 
GAAGGAATGG AAGTTAATCA AGAAGAGAAG AGGGAGGCTG ATCGGGGAGA AATAGGGGAT 
GGAGCATCGG TAGATCTCCC TGATGGGAAA CATTCGTTCC CCGTCTCGCT CCGGATCTCA 
AGAGGACCCA TTGCTGGGCC CCCTTTGCCC GAGACAAACA CACACAGTCT CCCTCCTGGA 
GATTTCCCTG CCAACGTGAC CTTTATTAAG GGGAACTATG TGCTGGAGAG TGATGTGCTG 
CTGCAGACAC AGAGGGAGGA GTACGATGTC ATCCTGTGTT TGAGTGTGAC AAAGTGGGTC 
CACTTGAACT GGGGCGATGC AGGACTCAAG CGCTTCTTTC ACAGGGTATA CAAGCACCTG 
CGGCCAGGGG GACTCTTCAT TCTGGAGCCC CAGCCCTGGA GCTCCTACAA CAAGAGGAAG 
AAGCTCACCG AGGCCATCTG TAAGAATTAC CACAGCATCC GGTTAAAACC AGACCAGTTC 
TCCTCTTTCC TGACGACTGA GGTGGGCTTC TCCAGTTATG AGCTCATTGG GACGTCGCAA 
AACTACTCCA AAGGTTTTCA GAGACCAATC TCTCTGTATC ACAAACGACC TTCATCTTTA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mepce ( XM_021476212.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331887.1 Danio rerio methylphosphate capping enzyme (mepce), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00154
Clone ID Related Accession (Same CDS sequence)	NM_001128529.1 , XM_021476212.1
Accession Version	XM_021476212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	7SK snRNA methylphosphate capping enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGAGA TGTCATTTGA CAAAGAAACT GTTTTATCTT GTGAGGGGCA AACAGACCCC 
ACTTCTCAAG AATTTAATAT AAATGATACC AGTGCTCTCC ATCCACCCGG GAACACCACA 
GAGAACAACC TCAGTAAGGA CGCAGGAAGT GAGGCATCCC TAGAGAAATC TGCTACCACC 
GATAACAATG CTTCAAGTGA AAGTGCAGTT CTTGGGGCTG AATCGTCTAA ACAATCGCAC 
AATGGCACCA AACCACAACA GCAGAAAGTC AACAAGCGCC GCAACACTAT GAGCGCTGGG 
TTTAAGCACC CGGGTTATGG CAAACGACGA CGGCGAGCCA ACTCGGAAAG CGAGTCAGTC 
CTGCCAACCA ATTTCCTGCT GGGCGGGAAC ATTTTTGACC CACTCAACCT TAACAGCCTT 
TTAGACGAAG AGGTCAACAA GGCTCTCAAT GCAGAGACGC CCAAGTCTTC GCCATTGCCA 
GCCAAAAACC GAGACCCTGT TGAGATCTTG ATACCCAGAG ATATCACAGA CCCCCTCAAC 
CTCAACTGCC TGAGCACAGG AGACAGTCTG CTGGTGTCTC CATTGAAGAG TGGTGGAGGA 
AGGCGGAGAC ATCGAAACAA ACACCATGGA GCAAACACTG GACGAGTTTC CACTGAAGGA 
ATTGGTTTAA CGGAGTCTAA TGGCTGTGCA GCAGTGAAGG ATGATGGAAC ATCATTGGTC 
TCGATGACTA ATGTGCATTT AGAAAGTACA ATTGCGGATC AAACTCTGTT GGACTCGACT 
CTCGAGTCTT CAAATAAGTC CAGTCTAACT TCAAATGACA ATGAGGTGAA GGAGAAAACC 
GTTGACTGCT CAACCGCACA TCTAACAGCA AACCCAACCA ACCCTCCATC AAGCAGGCAG 
CGCAAACGCA GGCGAGCCGG CAGCAGAACG GAGAACTCCA GTTCTTCTTC TGAGGTGAAA 
AGATCGGGGA GACATTTTCA ACACAATGAA TCCCAAGGAG GAAACTCTCG AAGTGGAGTC 
TCTGATAGGC AGCAACCGTA TCCGAATCAA TGGAGGCCGC AGTACAGCAA CAACAATCAG 
AACCCGCAGC AGCCCAAAAA GAAGTTTCAA TACGGTAACT ATAACAAATA CTACGGCTAT 
CGGAACCCAG GGATGAGCGA GGATCCTCGC ATTCGTGTTA TGAATCCGGA TTGGTTCAGA 
GGGAAGGATG TGTTGGATCT GGGATGCAAC ACTGGCCACC TGACTCTGTT TATCGCAAAA 
AATTGGCGGC CTGCTAGTAT TGTGGGCCTG GATATTGACG GATCGCTGAT ACATGCTGCT 
CGACAGAATA TAAGACACTA CCTCTCTGAG GTCCAAGTGC AGCACTCCCG ACGCTCTGGG 
GAAAATACAA AGGCAGATCG AGGGGAGGTT TCGGGAGAAG AAAAGGACAA AGATAAAACT 
AGTTTGGTTA TGGATGAAAA AAAGGCAAAG CATGTTGACG AAGTGAATAA ATGCATGGAC 
GAAGGAATGG AAGTTAATCA AGAAGAGAAG AGGGAGGCTG ATCGGGGAGA AATAGGGGAT 
GGAGCATCGG TAGATCTCCC TGATGGGAAA CATTCGTTCC CCGTCTCGCT CCGGATCTCA 
AGAGGACCCA TTGCTGGGCC CCCTTTGCCC GAGACAAACA CACACAGTCT CCCTCCTGGA 
GATTTCCCTG CCAACGTGAC CTTTATTAAG GGGAACTATG TGCTGGAGAG TGATGTGCTG 
CTGCAGACAC AGAGGGAGGA GTACGATGTC ATCCTGTGTT TGAGTGTGAC AAAGTGGGTC 
CACTTGAACT GGGGCGATGC AGGACTCAAG CGCTTCTTTC ACAGGGTATA CAAGCACCTG 
CGGCCAGGGG GACTCTTCAT TCTGGAGCCC CAGCCCTGGA GCTCCTACAA CAAGAGGAAG 
AAGCTCACCG AGGCCATCTG TAAGAATTAC CACAGCATCC GGTTAAAACC AGACCAGTTC 
TCCTCTTTCC TGACGACTGA GGTGGGCTTC TCCAGTTATG AGCTCATTGG GACGTCGCAA 
AACTACTCCA AAGGTTTTCA GAGACCAATC TCTCTGTATC ACAAACGACC TTCATCTTTA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331887.1
CDS	439..2544
Translation	MIEMSFDKETVLSCEGQTDPTSQEFNINDTSALHPPGNTTENNLSKDAGSEASLEKSATTDNNASSESAVLGAESSKQSHNGTKPQQQKVNKRRNTMSAGFKHPGYGKRRRRANSESESVLPTNFLLGGNIFDPLNLNSLLDEEVNKALNAETPKSSPLPAKNRDPVEILIPRDITDPLNLNCLSTGDSLLVSPLKSGGGRRRHRNKHHGANTGRVSTEGIGLTESNGCAAVKDDGTSLVSMTNVHLESTIADQTLLDSTLESSNKSSLTSNDNEVKEKTVDCSTAHLTANPTNPPSSRQRKRRRAGSRTENSSSSSEVKRSGRHFQHNESQGGNSRSGVSDRQQPYPNQWRPQYSNNNQNPQQPKKKFQYGNYNKYYGYRNPGMSEDPRIRVMNPDWFRGKDVLDLGCNTGHLTLFIAKNWRPASIVGLDIDGSLIHAARQNIRHYLSEVQVQHSRRSGENTKADRGEVSGEEKDKDKTSLVMDEKKAKHVDEVNKCMDEGMEVNQEEKREADRGEIGDGASVDLPDGKHSFPVSLRISRGPIAGPPLPETNTHSLPPGDFPANVTFIKGNYVLESDVLLQTQREEYDVILCLSVTKWVHLNWGDAGLKRFFHRVYKHLRPGGLFILEPQPWSSYNKRKKLTEAICKNYHSIRLKPDQFSSFLTTEVGFSSYELIGTSQNYSKGFQRPISLYHKRPSSLK

Target ORF information:

RefSeq Version	XM_021476212.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylphosphate capping enzyme (mepce), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476212.1

ORF Insert Sequence:

ATGATAGAGA TGTCATTTGA CAAAGAAACT GTTTTATCTT GTGAGGGGCA AACAGACCCC 
ACTTCTCAAG AATTTAATAT AAATGATACC AGTGCTCTCC ATCCACCCGG GAACACCACA 
GAGAACAACC TCAGTAAGGA CGCAGGAAGT GAGGCATCCC TAGAGAAATC TGCTACCACC 
GATAACAATG CTTCAAGTGA AAGTGCAGTT CTTGGGGCTG AATCGTCTAA ACAATCGCAC 
AATGGCACCA AACCACAACA GCAGAAAGTC AACAAGCGCC GCAACACTAT GAGCGCTGGG 
TTTAAGCACC CGGGTTATGG CAAACGACGA CGGCGAGCCA ACTCGGAAAG CGAGTCAGTC 
CTGCCAACCA ATTTCCTGCT GGGCGGGAAC ATTTTTGACC CACTCAACCT TAACAGCCTT 
TTAGACGAAG AGGTCAACAA GGCTCTCAAT GCAGAGACGC CCAAGTCTTC GCCATTGCCA 
GCCAAAAACC GAGACCCTGT TGAGATCTTG ATACCCAGAG ATATCACAGA CCCCCTCAAC 
CTCAACTGCC TGAGCACAGG AGACAGTCTG CTGGTGTCTC CATTGAAGAG TGGTGGAGGA 
AGGCGGAGAC ATCGAAACAA ACACCATGGA GCAAACACTG GACGAGTTTC CACTGAAGGA 
ATTGGTTTAA CGGAGTCTAA TGGCTGTGCA GCAGTGAAGG ATGATGGAAC ATCATTGGTC 
TCGATGACTA ATGTGCATTT AGAAAGTACA ATTGCGGATC AAACTCTGTT GGACTCGACT 
CTCGAGTCTT CAAATAAGTC CAGTCTAACT TCAAATGACA ATGAGGTGAA GGAGAAAACC 
GTTGACTGCT CAACCGCACA TCTAACAGCA AACCCAACCA ACCCTCCATC AAGCAGGCAG 
CGCAAACGCA GGCGAGCCGG CAGCAGAACG GAGAACTCCA GTTCTTCTTC TGAGGTGAAA 
AGATCGGGGA GACATTTTCA ACACAATGAA TCCCAAGGAG GAAACTCTCG AAGTGGAGTC 
TCTGATAGGC AGCAACCGTA TCCGAATCAA TGGAGGCCGC AGTACAGCAA CAACAATCAG 
AACCCGCAGC AGCCCAAAAA GAAGTTTCAA TACGGTAACT ATAACAAATA CTACGGCTAT 
CGGAACCCAG GGATGAGCGA GGATCCTCGC ATTCGTGTTA TGAATCCGGA TTGGTTCAGA 
GGGAAGGATG TGTTGGATCT GGGATGCAAC ACTGGCCACC TGACTCTGTT TATCGCAAAA 
AATTGGCGGC CTGCTAGTAT TGTGGGCCTG GATATTGACG GATCGCTGAT ACATGCTGCT 
CGACAGAATA TAAGACACTA CCTCTCTGAG GTCCAAGTGC AGCACTCCCG ACGCTCTGGG 
GAAAATACAA AGGCAGATCG AGGGGAGGTT TCGGGAGAAG AAAAGGACAA AGATAAAACT 
AGTTTGGTTA TGGATGAAAA AAAGGCAAAG CATGTTGACG AAGTGAATAA ATGCATGGAC 
GAAGGAATGG AAGTTAATCA AGAAGAGAAG AGGGAGGCTG ATCGGGGAGA AATAGGGGAT 
GGAGCATCGG TAGATCTCCC TGATGGGAAA CATTCGTTCC CCGTCTCGCT CCGGATCTCA 
AGAGGACCCA TTGCTGGGCC CCCTTTGCCC GAGACAAACA CACACAGTCT CCCTCCTGGA 
GATTTCCCTG CCAACGTGAC CTTTATTAAG GGGAACTATG TGCTGGAGAG TGATGTGCTG 
CTGCAGACAC AGAGGGAGGA GTACGATGTC ATCCTGTGTT TGAGTGTGAC AAAGTGGGTC 
CACTTGAACT GGGGCGATGC AGGACTCAAG CGCTTCTTTC ACAGGGTATA CAAGCACCTG 
CGGCCAGGGG GACTCTTCAT TCTGGAGCCC CAGCCCTGGA GCTCCTACAA CAAGAGGAAG 
AAGCTCACCG AGGCCATCTG TAAGAATTAC CACAGCATCC GGTTAAAACC AGACCAGTTC 
TCCTCTTTCC TGACGACTGA GGTGGGCTTC TCCAGTTATG AGCTCATTGG GACGTCGCAA 
AACTACTCCA AAGGTTTTCA GAGACCAATC TCTCTGTATC ACAAACGACC TTCATCTTTA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.
Proceedings of the National Academy of Sciences of the United States of America106(19)7798-803(2009 May)
Barboric M,Lenasi T,Chen H,Johansen EB,Guo S,Peterlin BM

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary.
Gene294(1-2)45-53(2002 Jul)
Zeng S,Gong Z

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mepce cDNA ORF clone, Danio rerio(zebrafish)

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