sema5a cDNA ORF clone, Danio rerio(zebrafish)

The following sema5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa43475 XM_021470194.1
Latest version!
Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa35434 NM_001271670.1
Latest version!
Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa28490 XM_017353840.1
Latest version!
Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa28490 XM_005162619.4
Latest version!
Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa28490 XM_005162619.3 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa43475
    Clone ID Related Accession (Same CDS sequence) XM_021470194.1
    Accession Version XM_021470194.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
    ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA
    GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT
    GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG
    GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA
    GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG
    GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA
    ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT
    GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC
    ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA
    GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA
    TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC
    TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC
    GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG
    AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG
    GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC
    AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC
    GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC
    CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT
    CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG
    CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC
    AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG
    CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC
    CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT
    GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT
    AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA
    TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT
    CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT
    AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT
    CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC
    TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG
    ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC
    GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG
    CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA
    ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA
    TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA
    TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC
    TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG
    AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT
    GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG
    TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA
    CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC
    ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG
    ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT
    GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT
    CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT
    CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT
    GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA
    AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG
    AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC
    CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC
    AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGCCT
    GATGTTTCCG TTCTCTTGCC TTTGGACGAG CACCACATTT GCCAGCCTTT GTCTTCTAAC
    ACGGGTGTAG CGCGAGGCCT GGAGATTTTC TACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325869.1
    CDS465..3680
    Translation

    Target ORF information:

    RefSeq Version XM_021470194.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470194.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
    ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA
    GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT
    GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG
    GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA
    GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG
    GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA
    ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT
    GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC
    ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA
    GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA
    TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC
    TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC
    GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG
    AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG
    GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC
    AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC
    GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC
    CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT
    CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG
    CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC
    AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG
    CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC
    CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT
    GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT
    AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA
    TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT
    CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT
    AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT
    CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC
    TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG
    ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC
    GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG
    CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA
    ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA
    TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA
    TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC
    TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG
    AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT
    GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG
    TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA
    CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC
    ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG
    ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT
    GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT
    CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT
    CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT
    GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA
    AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG
    AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC
    CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC
    AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGCCT
    GATGTTTCCG TTCTCTTGCC TTTGGACGAG CACCACATTT GCCAGCCTTT GTCTTCTAAC
    ACGGGTGTAG CGCGAGGCCT GGAGATTTTC TACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa35434
    Clone ID Related Accession (Same CDS sequence) NM_001271670.1
    Accession Version NM_001271670.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product semaphorin-5A
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX323577.16, CR354550.8 and CT573359.10. On Nov 4, 2012 this sequence version replaced XM_001922538.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTCCTGG TGCAGATTCC TTGTCCTCAT CTATTCTTGA ATGCTGCCCG TTTGTGTCAG 
    GATCCAGTTC ACCTGGCTAA GGATGCAACA AAAACAGGCT CAATCTCCAG AATGCAAACC
    ATTCTGGTGG AAAGAAGGTG TCCGATGGTG CAGTCAAACT CTGGTTCAAA ACCAAAAAAA
    GAACCATCAA CAATATCCAG AGAGGAGGCA GAATGGCATT GTGATGAGTT TACCAAAGGA
    GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG GAGTGCCAGA ATTACATCCG TGTCCTCCTC
    GTCAATGGTG ACAGGCTGTT TACCTGCGGA ACAAATGCAT TCACTCCTAT TTGCACCAAT
    CGCACGCTGA CTAACCTGAC TGAGGTCCAT GATCAAATCA GTGGGATGGC ACGGTGCCCC
    TATAACCCTC TGCATAATTC CACCGCCCTC ATCACTTCCA GTGGAGAACT GTATGCTGCA
    ACTGCAATGG ACTTTTCAGG CAGAGACCCA GCCATCTACC GCAGCTTGGG AGGGCTTCCA
    CCTCTGCGTA CTGCTCAGTA CAACTCCAAA TGGCTCAATG AGCCCAACTT CGTCTCCTCC
    TATGACATCG GCAACTTCAC GTACTTCTTC TTCCGTGAAA ATGCTGTGGA GCACGACTGC
    GGCAGGACTG TTTTCTCTCG GGCTGCCCGC GTCTGCAAGA ATGATATCGG GGGCCGTTTC
    CTTCTGGAGG ACACCTGGAC TACCTTTATG AAAGCCCGGC TCAACTGCTC ACGGCCTGGC
    GAGATCCCAT TCAACTACAA TGAGTTGCAG GGAACCTTCT TTCTGCCTGA GCTCGAGCTC
    CTCTATGGGA TTTTCACCAC TAATGTTAAC AGTATTGCGG CCTCAGCAGT GTGTGCCTTC
    AATCTGAGCG CTATTACCCA AGTGTTCAGC GGCCCTTTCA AGTACCAAGA GAACTCGCGC
    TCTGCTTGGC TTCCTTACCC CAATCCTAAC CCCGACTTCC AGTGTGGTAC TATAGATTTT
    GGCTCGTATG TGAACTTAAC GGAGAGGAAT CTGCAGGATG CTCAGAAGTT CATCCTGATG
    CATGAGGTGG TGCAGCCTGT GGTTCCTGTG CCGTATTTCA TGGAGGACAA TGTGCGCTTC
    TCTCATGTGG CTGTGGACGT GGTGCAGGGC AAAGACATGC TTTACCACAT CATTTATCTG
    GCAACAGATT ACGGCACCAT TAAGAAGGTG CTCTCCCCTC TCAACCAGAC CACGGGCAGC
    TGCTTGCTGG ACGAGATTGA GCTTTTCCCC CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG
    CTCATCCTTC ACAGCCACAG TGAGCTATTT GTAGGAGTCA GAGAGCAGGT CATCAAAATC
    CCCCTGATGC GCTGCAACTT CCACAAGTCT AGAGAAGCCT GTGTGGGGGC CAGAGATCCA
    TACTGCGGCT GGGATCTGGT GCTCAAGAAA TGCACCACGC TGGAAGAGAG CGTCAGCATG
    AGCCAATGGG AGCAGAGCAT TACACGCTGT CCTGTGAGAA ACGTGACTGT AGATGGTCAT
    TATGGAGCCT GGTCAGGGTG GAAAACTTGT AGTCACAGTG ATGGTGGCAG TGTGGGTTCG
    TGCCAGTGTC GAACCCGAGC GTGTGACAGT CCAAGTCCTC AATGTGGCGG ACAGCCTTGC
    CAAGGAATCA GTGTTGAAGT GGCAAACTGC TCCAGAAATG GGGCATGGAC ACCGTGGACT
    GCCTGGGCTC CATGCAGCAC CAGCTGTGGG ATTGGATTCC AGGTTCGGCA GCGTTCCTGC
    AGTAACCCCA CTCCCAGACA TGGAGGACGC GTCTGTGTGG GCCAGAACCG AGAGGAGAGA
    TACTGCAATG AACACCTGCC GTGTCCTCCG CATGTCTATT GGTCAGCTTG GTCTCCTTGG
    GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT
    GGAAATGAAT GCCCTGGATG CAGCACTGAA TTCCAGTCCT GTAACACCCT CCCCTGTCCG
    GATCTGAAGA AGACCACGCC CTGGACCCCA TGGACCCCAG TGAATATCTC AGACAATGGT
    GGCCACTACG AGCAGCGCTT CAGATACACC TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG
    CTGGAAGTTG GACGACAGCG AATCGAAATG AGGTACTGCT CCAGCGACGG CTCCACGGGC
    TGCTCCACTG ACGGAGATTT GCTGCGTTCT GGACGTATCA CTGGATTGAC AGTAAATGGA
    GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA
    AATCGCAAAC GCACCTGCTC CAACCCCAAA CCCAAGTATG GAGGAATTAC CTGCCTCGGG
    CCAGCCCAAG AGTTCCAGGA GTGCAATACC ACACCATGTC CAGTGGACGG GAGCTGGTCG
    TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG ACATGCGGCG GAGGCCATTA CATGCGCACA
    CGCTCCTGTA ACAACCCTCC ACCTGCCTAT GGAGGTGACA TCTGCCTGGG TTTGCATACT
    GAAGAGGCCC TCTGCAACAT CCAGCCCTGT CCTGAGAGCT GGTCTGATTG GTCAGAATGG
    TCCCCCTGTG ACTCATCAGG GTCTCAGGTT CGCCTCCGGC ATTGTGACGT CTTGTTTCCC
    ACCGGCAACC AGTGCTCTGG CAACAGCAGT GAAACTCGTG CCTGTCTGCC CATCTCCAAC
    TTCATCCCAG AAACGTCTGT TGCACGTTCA AGTCAGGAGG AAAGATGGTG TGGAGACTTC
    AATGTGTTCC ACATGATCGC CGTGGGTCTG AGCAGCTCCA TACTCGGCTG CTTGGTCACT
    TTACTGGTGT ACACATACTG CCAGAGATAC CAGCAGCAAT CCCATGATGC CACAGTCATC
    CATCCTATCT CTGCTGCCCC GCTCAACACC AGCATCAGCA ACCATATCAA CAAACTGGAC
    AAGTATGACT CAGTTGAAGC CATTAAGGCT TTCAATAAGA ATAACTTGAT CCTGGAGGAA
    AGAAATAAGT ATTTCAACCC TCAGCTAGCA GGAAAAACAT ATACCAACGC ATATTTTACT
    GACGTCAACA CATACGACGA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258599.1
    CDS265..3411
    Translation

    Target ORF information:

    RefSeq Version NM_001271670.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271670.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTCCTGG TGCAGATTCC TTGTCCTCAT CTATTCTTGA ATGCTGCCCG TTTGTGTCAG 
    GATCCAGTTC ACCTGGCTAA GGATGCAACA AAAACAGGCT CAATCTCCAG AATGCAAACC
    ATTCTGGTGG AAAGAAGGTG TCCGATGGTG CAGTCAAACT CTGGTTCAAA ACCAAAAAAA
    GAACCATCAA CAATATCCAG AGAGGAGGCA GAATGGCATT GTGATGAGTT TACCAAAGGA
    GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG GAGTGCCAGA ATTACATCCG TGTCCTCCTC
    GTCAATGGTG ACAGGCTGTT TACCTGCGGA ACAAATGCAT TCACTCCTAT TTGCACCAAT
    CGCACGCTGA CTAACCTGAC TGAGGTCCAT GATCAAATCA GTGGGATGGC ACGGTGCCCC
    TATAACCCTC TGCATAATTC CACCGCCCTC ATCACTTCCA GTGGAGAACT GTATGCTGCA
    ACTGCAATGG ACTTTTCAGG CAGAGACCCA GCCATCTACC GCAGCTTGGG AGGGCTTCCA
    CCTCTGCGTA CTGCTCAGTA CAACTCCAAA TGGCTCAATG AGCCCAACTT CGTCTCCTCC
    TATGACATCG GCAACTTCAC GTACTTCTTC TTCCGTGAAA ATGCTGTGGA GCACGACTGC
    GGCAGGACTG TTTTCTCTCG GGCTGCCCGC GTCTGCAAGA ATGATATCGG GGGCCGTTTC
    CTTCTGGAGG ACACCTGGAC TACCTTTATG AAAGCCCGGC TCAACTGCTC ACGGCCTGGC
    GAGATCCCAT TCAACTACAA TGAGTTGCAG GGAACCTTCT TTCTGCCTGA GCTCGAGCTC
    CTCTATGGGA TTTTCACCAC TAATGTTAAC AGTATTGCGG CCTCAGCAGT GTGTGCCTTC
    AATCTGAGCG CTATTACCCA AGTGTTCAGC GGCCCTTTCA AGTACCAAGA GAACTCGCGC
    TCTGCTTGGC TTCCTTACCC CAATCCTAAC CCCGACTTCC AGTGTGGTAC TATAGATTTT
    GGCTCGTATG TGAACTTAAC GGAGAGGAAT CTGCAGGATG CTCAGAAGTT CATCCTGATG
    CATGAGGTGG TGCAGCCTGT GGTTCCTGTG CCGTATTTCA TGGAGGACAA TGTGCGCTTC
    TCTCATGTGG CTGTGGACGT GGTGCAGGGC AAAGACATGC TTTACCACAT CATTTATCTG
    GCAACAGATT ACGGCACCAT TAAGAAGGTG CTCTCCCCTC TCAACCAGAC CACGGGCAGC
    TGCTTGCTGG ACGAGATTGA GCTTTTCCCC CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG
    CTCATCCTTC ACAGCCACAG TGAGCTATTT GTAGGAGTCA GAGAGCAGGT CATCAAAATC
    CCCCTGATGC GCTGCAACTT CCACAAGTCT AGAGAAGCCT GTGTGGGGGC CAGAGATCCA
    TACTGCGGCT GGGATCTGGT GCTCAAGAAA TGCACCACGC TGGAAGAGAG CGTCAGCATG
    AGCCAATGGG AGCAGAGCAT TACACGCTGT CCTGTGAGAA ACGTGACTGT AGATGGTCAT
    TATGGAGCCT GGTCAGGGTG GAAAACTTGT AGTCACAGTG ATGGTGGCAG TGTGGGTTCG
    TGCCAGTGTC GAACCCGAGC GTGTGACAGT CCAAGTCCTC AATGTGGCGG ACAGCCTTGC
    CAAGGAATCA GTGTTGAAGT GGCAAACTGC TCCAGAAATG GGGCATGGAC ACCGTGGACT
    GCCTGGGCTC CATGCAGCAC CAGCTGTGGG ATTGGATTCC AGGTTCGGCA GCGTTCCTGC
    AGTAACCCCA CTCCCAGACA TGGAGGACGC GTCTGTGTGG GCCAGAACCG AGAGGAGAGA
    TACTGCAATG AACACCTGCC GTGTCCTCCG CATGTCTATT GGTCAGCTTG GTCTCCTTGG
    GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT
    GGAAATGAAT GCCCTGGATG CAGCACTGAA TTCCAGTCCT GTAACACCCT CCCCTGTCCG
    GATCTGAAGA AGACCACGCC CTGGACCCCA TGGACCCCAG TGAATATCTC AGACAATGGT
    GGCCACTACG AGCAGCGCTT CAGATACACC TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG
    CTGGAAGTTG GACGACAGCG AATCGAAATG AGGTACTGCT CCAGCGACGG CTCCACGGGC
    TGCTCCACTG ACGGAGATTT GCTGCGTTCT GGACGTATCA CTGGATTGAC AGTAAATGGA
    GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA
    AATCGCAAAC GCACCTGCTC CAACCCCAAA CCCAAGTATG GAGGAATTAC CTGCCTCGGG
    CCAGCCCAAG AGTTCCAGGA GTGCAATACC ACACCATGTC CAGTGGACGG GAGCTGGTCG
    TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG ACATGCGGCG GAGGCCATTA CATGCGCACA
    CGCTCCTGTA ACAACCCTCC ACCTGCCTAT GGAGGTGACA TCTGCCTGGG TTTGCATACT
    GAAGAGGCCC TCTGCAACAT CCAGCCCTGT CCTGAGAGCT GGTCTGATTG GTCAGAATGG
    TCCCCCTGTG ACTCATCAGG GTCTCAGGTT CGCCTCCGGC ATTGTGACGT CTTGTTTCCC
    ACCGGCAACC AGTGCTCTGG CAACAGCAGT GAAACTCGTG CCTGTCTGCC CATCTCCAAC
    TTCATCCCAG AAACGTCTGT TGCACGTTCA AGTCAGGAGG AAAGATGGTG TGGAGACTTC
    AATGTGTTCC ACATGATCGC CGTGGGTCTG AGCAGCTCCA TACTCGGCTG CTTGGTCACT
    TTACTGGTGT ACACATACTG CCAGAGATAC CAGCAGCAAT CCCATGATGC CACAGTCATC
    CATCCTATCT CTGCTGCCCC GCTCAACACC AGCATCAGCA ACCATATCAA CAAACTGGAC
    AAGTATGACT CAGTTGAAGC CATTAAGGCT TTCAATAAGA ATAACTTGAT CCTGGAGGAA
    AGAAATAAGT ATTTCAACCC TCAGCTAGCA GGAAAAACAT ATACCAACGC ATATTTTACT
    GACGTCAACA CATACGACGA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28490
    Clone ID Related Accession (Same CDS sequence) XM_017353840.1 , XM_005162619.3 , XM_005162619.4
    Accession Version XM_017353840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-5A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
    ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA
    GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT
    GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG
    GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA
    GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG
    GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA
    ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT
    GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC
    ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA
    GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA
    TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC
    TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC
    GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG
    AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG
    GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC
    AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC
    GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC
    CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT
    CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG
    CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC
    AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG
    CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC
    CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT
    GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT
    AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA
    TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT
    CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT
    AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT
    CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC
    TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG
    ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC
    GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG
    CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA
    ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA
    TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA
    TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC
    TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG
    AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT
    GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG
    TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA
    CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC
    ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG
    ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT
    GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT
    CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT
    CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT
    GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA
    AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG
    AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC
    CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC
    AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT
    TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA
    GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209329.1
    CDS112..3348
    Translation

    Target ORF information:

    RefSeq Version XM_017353840.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
    ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA
    GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT
    GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG
    GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA
    GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG
    GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA
    ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT
    GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC
    ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA
    GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA
    TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC
    TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC
    GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG
    AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG
    GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC
    AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC
    GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC
    CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT
    CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG
    CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC
    AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG
    CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC
    CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT
    GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT
    AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA
    TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT
    CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT
    AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT
    CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC
    TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG
    ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC
    GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG
    CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA
    ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA
    TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA
    TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC
    TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG
    AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT
    GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG
    TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA
    CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC
    ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG
    ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT
    GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT
    CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT
    CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT
    GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA
    AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG
    AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC
    CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC
    AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT
    TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA
    GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28490
    Clone ID Related Accession (Same CDS sequence) XM_017353840.1 , XM_005162619.3 , XM_005162619.4
    Accession Version XM_005162619.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-5A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
    ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA
    GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT
    GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG
    GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA
    GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG
    GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA
    ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT
    GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC
    ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA
    GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA
    TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC
    TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC
    GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG
    AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG
    GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC
    AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC
    GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC
    CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT
    CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG
    CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC
    AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG
    CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC
    CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT
    GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT
    AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA
    TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT
    CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT
    AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT
    CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC
    TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG
    ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC
    GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG
    CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA
    ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA
    TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA
    TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC
    TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG
    AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT
    GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG
    TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA
    CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC
    ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG
    ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT
    GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT
    CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT
    CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT
    GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA
    AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG
    AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC
    CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC
    AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT
    TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA
    GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162676.1
    CDS442..3678
    Translation

    Target ORF information:

    RefSeq Version XM_005162619.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162619.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
    ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA
    GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT
    GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG
    GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA
    GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG
    GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA
    ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT
    GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC
    ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA
    GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA
    TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC
    TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC
    GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG
    AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG
    GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC
    AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC
    GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC
    CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT
    CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG
    CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC
    AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG
    CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC
    CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT
    GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT
    AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA
    TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT
    CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT
    AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT
    CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC
    TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG
    ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC
    GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG
    CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA
    ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA
    TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA
    TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC
    TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG
    AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT
    GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG
    TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA
    CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC
    ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG
    ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT
    GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT
    CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT
    CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT
    GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA
    AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG
    AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC
    CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC
    AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT
    TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA
    GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28490
    Clone ID Related Accession (Same CDS sequence) XM_017353840.1 , XM_005162619.3 , XM_005162619.4
    Accession Version XM_005162619.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162619.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
    ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA
    GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT
    GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG
    GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA
    GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG
    GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA
    ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT
    GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC
    ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA
    GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA
    TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC
    TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC
    GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG
    AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG
    GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC
    AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC
    GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC
    CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT
    CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG
    CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC
    AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG
    CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC
    CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT
    GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT
    AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA
    TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT
    CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT
    AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT
    CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC
    TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG
    ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC
    GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG
    CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA
    ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA
    TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA
    TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC
    TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG
    AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT
    GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG
    TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA
    CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC
    ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG
    ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT
    GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT
    CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT
    CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT
    GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA
    AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG
    AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC
    CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC
    AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT
    TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA
    GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162676.1
    CDS463..3699
    Translation

    Target ORF information:

    RefSeq Version XM_005162619.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162619.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
    ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA
    GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT
    GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG
    GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA
    GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG
    GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA
    ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT
    GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC
    ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA
    GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA
    TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC
    TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC
    GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG
    AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG
    GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC
    AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC
    GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC
    CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT
    CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG
    CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC
    AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG
    CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC
    CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT
    GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT
    AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA
    TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT
    CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT
    AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT
    CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC
    TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG
    ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC
    GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG
    CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA
    ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA
    TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA
    TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC
    TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG
    AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT
    GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG
    TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA
    CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC
    ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG
    ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT
    GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT
    CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT
    CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT
    GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA
    AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG
    AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC
    CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC
    AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT
    TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA
    GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.