si:ch211-79l20.4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:ch211-79l20.4
Entrez Gene ID	560260
Full Name	vir like m6A methyltransferase associated
Synonyms	fa20f07,si:ch211-79l20.4,wu:fa20f07
General protein information	Preferred Names vir like m6A methyltransferase associated Names protein virilizer homolog vir like m6A methyltransferase associated
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005158035.4	XP_005158092.1	protein virilizer homolog isoform X2
XM_005158034.3	XP_005158091.1	protein virilizer homolog isoform X1
XM_683656.7	XP_688748.4	protein virilizer homolog isoform X3
XM_005158034.4	XP_005158091.1	protein virilizer homolog isoform X1
XM_005158035.3	XP_005158092.1	protein virilizer homolog isoform X2
XM_683656.8	XP_688748.4	protein virilizer homolog isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KIAA1429	NP_001192355.1
Mus musculus (house mouse)	1110037F02Rik	NP_001074652.1
Rattus norvegicus (Norway rat)	RGD1559904	NP_001101385.1
Danio rerio (zebrafish)	si:ch211-79l20.4	XP_005158091.1
	KIAA1429	XP_001089437.1
Homo sapiens (human)	KIAA1429	NP_056311.2
Canis lupus familiaris (dog)	KIAA1429	XP_005638215.1
Gallus gallus (chicken)	KIAA1429	XP_418337.3
Pan troglodytes (chimpanzee)	KIAA1429	XP_519860.2
Xenopus tropicalis (tropical clawed frog)	kiaa1429	XP_002934458.2

Gene Ontology

The following si:ch211-79l20.4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-79l20.4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa21521	XM_005158034.4 Latest version!	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa21522	XM_005158035.4 Latest version!	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa21523	XM_683656.8 Latest version!	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa21521	XM_005158034.3	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa21522	XM_005158035.3	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa21523	XM_683656.7	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:ch211-79l20.4 ( XM_005158034.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158091.1 Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X1, mRNA.

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CloneID	ODa21521
Clone ID Related Accession (Same CDS sequence)	XM_005158034.3 , XM_005158034.4
Accession Version	XM_005158034.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein virilizer homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGTTT CTCAGATCTG CATTCTATAG GCATCATCAC 
GCAGATGAAG ACATGGACAG CCGCTCTTTG GCTCTTAGCG TGAGTGACAT GAAGCAGCTG 
TTGCAGTGGA AGGAGGACTC GGGAGAGCAT CTGCTATGTG AACTGGAGAA ACATATTACG 
AAGCTCTGTA AGGAGGACGA GTCTTTGGAG GCTCTGCTTG AGAATGTAGT TGGACTGCGA 
CAGATTATTG AAAGTTCTGG AGACGCACAG ACTAATGCTG AGCCTGAGAC TGAGCCCTCT 
CTACCTGCGC CCGAGCCCCT TCAGACACAG TTCAACCACA GGACAGTGTA TGTACTTTCT 
GATGTCCTGG ATGAGCAGTT GAAGGCCCTG TGGTTTTCTC CATTCCAGCC AGATGAGATG 
GAGACAGAAG TAGACATGGT GAAAGTAGAC CTGGTTGCGC TGGCCCAGCA GTGTTGTACA 
GAATTAGACC TGAAGGCAGA GTTAGAGCGC TCATTCCTCA GTGAACCTTC TTCTCCAGGA 
CACAGCAAAC CAAGCAAAGG CTTCAGACTG GGCAAACACA AACACGAGAC CTTTATTACA 
TCCAGGTACA CAAGCGGAAG GTCTGACTAT GTGGAGCCAG CGAAGCGAGC TCATATCATG 
GCGGCCCCTC GGGGTCGTGG CCGCGGTTCG TTTGGCCAGC TCTCTCGACC ACATGACATT 
TTCCGCCAGC GCAAGCAGAA CACCAGTCGG CCTCCTTCCA TGCATGTAGA TGATTTCCTG 
GCTGCCGAGT TCAAGGATGT GGGTCCACCA ACAGGACTGA CGCTGCCCAA ACGCCCACCA 
AAGGGTGGAG GAGCCAAACC CCCCACCAGA GGCATGTTCA CTGGGGGAAC CAGAGGAAGA 
GGCTTCCAGA GTCACACACG CTTCTTCACT CCACCGGCCC CCAAGAGTGT GCTGCTCGCT 
GGTAACTATC CTCGTCGGGA AGGAGGTCGT GGATCCACTT GGAGCACACC AGTGACTGCT 
GTTACTCACA GAGGGACTTA CAGTGAGCCT CGTGGAGGAC AGAGCAACTT CACTCGACCA 
CTTCCTTCCA GACAGCCACC TACAGGTGCT TACAGACTGG CTCCTCGAGA CAGGGCCACC 
AGAGGCAGAG GGACGGGCCT GTCCTGGCTG AGCGCTGGAG TGAGTGTTGG AGGAGGCGGT 
GGGGTGAGCG GCGGTGGACG CGGCCAAGGC AAGTTCAGCA GCGGAGGCAG TGGAGGGGGA 
CGAGGCCGAC ACGTCCGCTC ATTCACTCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158091.1
CDS	20..5572
Translation	MAGDSAMELLFLDTFKHQNAELTNVDVVRFPYGVLITEVRVIPPGIKAHSNLPDSRAFGETAPHAFQLDLFFNNVSKPNSPVFDRLGSLEYDENKSIIFRPNGKINTDGLVLRGWYTCLTLAVYGTAERPHSHERDSPPPPPPPPPQQQQIGSKRLIKLEWENEDQFNGSPPRPQPRGPRTPPGPPPPDDDDEEAMPAPGQCPVKVENTEHPGDDYLEPVSPERGSLPADETYSDAEQEDDEGPADEEEDDVQSEGTEGDEDDEEDEVGDDVTDEDGEGDDGYEQISSDEEDLESGAFKLPAFDLDYTPEDLASLPTVQYDPYERELRPLQHFTPPHITRYEAELKRLKTVDIEDSSSSWAESAAKLSELLEAWGEAQGAERGAGWVTALEEVPAFLVKGMSFLSIKDPDGQSEKLKQLVDWSCEALSLDVALAQPIALNLRQLKAGTKLVSALADCGAQAVQDLLEKGIVGRLLGLLFAEHVSSTLKLNVLRSLDSIISEPQGIEAMLRDRHHHDDDGTSGYQRLLDLFLLDQTVRVATAGAAILHKGHLYEVLTDLQKTASVWAERHPTVGEVAEREGSESEAGERDRESTEREGEERERETQEAERESPMDIDTLLESASLSEADLERLQGLLEELLHLLETAPHCMVQPPGKAFPTNVRITGPPERDDPYPTLYRYMHACHLLESLGVVLSYPATCAHIGVLQAVRELMRFLSQSQQGLLFLLSQHETINLLLRLLTPLTHLTPPSHLAPLTPLSEVEGEEAAAGGEGLMDDGFWVWLMQTLHALQGIAELNGAELEDGDNPDVLATLHSLYLISFSSTGRNAVAHTFSLDNNLSCLINLIEFHAKDGQGDGKARKSVTYNYACMLVLLVIQTSSDLRMMEHYAAPLLLVCKADENNAKLQELSKWLEPLEKLHFDISGIPMLIDYIKQNLENLMTADGVGLVTALRVLCHIACPPATVPGQQKDLKWNQAIIALFSAEGMDTFIKVLQKLTSLLLLPWRLHGPMGPTAQRLMMLSVASCSLRLIRAMLTELLCGGTCEFRDVRVPSVCVTLHKILCSTPSTGRLDAEELRIQGDVTEILLTFTHAVSEQETGSEETVAGNSWSLMLKEVLNSILAAPENIYSSLSLLSELLPLPLPMQTTQALSVQDIGVAMNTRKLWSVHLHAQARVLQEVLRCACVSSCPPLFSMLRRVCVQLCDLAAPTATLVMRTLLEQLQEEMQQEAAGSRLIRVLALLDSLASQRACKVAMLALLSGSARSDTRSEGKVEDKLSDILPSLLAMLVLPADVGLSAQQNAELMASILQSLCDQDVSLVVSGSGEASVSEAELLANALPTKEMMTSICEGMLDVLGNTHSSYTLQLTCLRTMMFLTEHDYGLYHLKSSLKKHTTAISSLIRREITSFNKEFAELLSALFDFLRQILNSEAMFLRSAFYRHHHADEDMDSRSLALSVSDMKQLLQWKEDSGEHLLCELEKHITKLCKEDESLEALLENVVGLRQIIESSGDAQTNAEPETEPSLPAPEPLQTQFNHRTVYVLSDVLDEQLKALWFSPFQPDEMETEVDMVKVDLVALAQQCCTELDLKAELERSFLSEPSSPGHSKPSKGFRLGKHKHETFITSRYTSGRSDYVEPAKRAHIMAAPRGRGRGSFGQLSRPHDIFRQRKQNTSRPPSMHVDDFLAAEFKDVGPPTGLTLPKRPPKGGGAKPPTRGMFTGGTRGRGFQSHTRFFTPPAPKSVLLAGNYPRREGGRGSTWSTPVTAVTHRGTYSEPRGGQSNFTRPLPSRQPPTGAYRLAPRDRATRGRGTGLSWLSAGVSVGGGGGVSGGGRGQGKFSSGGSGGGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_005158034.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158034.3

ORF Insert Sequence:

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGTTT CTCAGATCTG CATTCTATAG GCATCATCAC 
GCAGATGAAG ACATGGACAG CCGCTCTTTG GCTCTTAGCG TGAGTGACAT GAAGCAGCTG 
TTGCAGTGGA AGGAGGACTC GGGAGAGCAT CTGCTATGTG AACTGGAGAA ACATATTACG 
AAGCTCTGTA AGGAGGACGA GTCTTTGGAG GCTCTGCTTG AGAATGTAGT TGGACTGCGA 
CAGATTATTG AAAGTTCTGG AGACGCACAG ACTAATGCTG AGCCTGAGAC TGAGCCCTCT 
CTACCTGCGC CCGAGCCCCT TCAGACACAG TTCAACCACA GGACAGTGTA TGTACTTTCT 
GATGTCCTGG ATGAGCAGTT GAAGGCCCTG TGGTTTTCTC CATTCCAGCC AGATGAGATG 
GAGACAGAAG TAGACATGGT GAAAGTAGAC CTGGTTGCGC TGGCCCAGCA GTGTTGTACA 
GAATTAGACC TGAAGGCAGA GTTAGAGCGC TCATTCCTCA GTGAACCTTC TTCTCCAGGA 
CACAGCAAAC CAAGCAAAGG CTTCAGACTG GGCAAACACA AACACGAGAC CTTTATTACA 
TCCAGGTACA CAAGCGGAAG GTCTGACTAT GTGGAGCCAG CGAAGCGAGC TCATATCATG 
GCGGCCCCTC GGGGTCGTGG CCGCGGTTCG TTTGGCCAGC TCTCTCGACC ACATGACATT 
TTCCGCCAGC GCAAGCAGAA CACCAGTCGG CCTCCTTCCA TGCATGTAGA TGATTTCCTG 
GCTGCCGAGT TCAAGGATGT GGGTCCACCA ACAGGACTGA CGCTGCCCAA ACGCCCACCA 
AAGGGTGGAG GAGCCAAACC CCCCACCAGA GGCATGTTCA CTGGGGGAAC CAGAGGAAGA 
GGCTTCCAGA GTCACACACG CTTCTTCACT CCACCGGCCC CCAAGAGTGT GCTGCTCGCT 
GGTAACTATC CTCGTCGGGA AGGAGGTCGT GGATCCACTT GGAGCACACC AGTGACTGCT 
GTTACTCACA GAGGGACTTA CAGTGAGCCT CGTGGAGGAC AGAGCAACTT CACTCGACCA 
CTTCCTTCCA GACAGCCACC TACAGGTGCT TACAGACTGG CTCCTCGAGA CAGGGCCACC 
AGAGGCAGAG GGACGGGCCT GTCCTGGCTG AGCGCTGGAG TGAGTGTTGG AGGAGGCGGT 
GGGGTGAGCG GCGGTGGACG CGGCCAAGGC AAGTTCAGCA GCGGAGGCAG TGGAGGGGGA 
CGAGGCCGAC ACGTCCGCTC ATTCACTCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-79l20.4 ( XM_005158034.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158091.1 Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X1, mRNA.

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CloneID	ODa21521
Clone ID Related Accession (Same CDS sequence)	XM_005158034.3 , XM_005158034.4
Accession Version	XM_005158034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein virilizer homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGTTT CTCAGATCTG CATTCTATAG GCATCATCAC 
GCAGATGAAG ACATGGACAG CCGCTCTTTG GCTCTTAGCG TGAGTGACAT GAAGCAGCTG 
TTGCAGTGGA AGGAGGACTC GGGAGAGCAT CTGCTATGTG AACTGGAGAA ACATATTACG 
AAGCTCTGTA AGGAGGACGA GTCTTTGGAG GCTCTGCTTG AGAATGTAGT TGGACTGCGA 
CAGATTATTG AAAGTTCTGG AGACGCACAG ACTAATGCTG AGCCTGAGAC TGAGCCCTCT 
CTACCTGCGC CCGAGCCCCT TCAGACACAG TTCAACCACA GGACAGTGTA TGTACTTTCT 
GATGTCCTGG ATGAGCAGTT GAAGGCCCTG TGGTTTTCTC CATTCCAGCC AGATGAGATG 
GAGACAGAAG TAGACATGGT GAAAGTAGAC CTGGTTGCGC TGGCCCAGCA GTGTTGTACA 
GAATTAGACC TGAAGGCAGA GTTAGAGCGC TCATTCCTCA GTGAACCTTC TTCTCCAGGA 
CACAGCAAAC CAAGCAAAGG CTTCAGACTG GGCAAACACA AACACGAGAC CTTTATTACA 
TCCAGGTACA CAAGCGGAAG GTCTGACTAT GTGGAGCCAG CGAAGCGAGC TCATATCATG 
GCGGCCCCTC GGGGTCGTGG CCGCGGTTCG TTTGGCCAGC TCTCTCGACC ACATGACATT 
TTCCGCCAGC GCAAGCAGAA CACCAGTCGG CCTCCTTCCA TGCATGTAGA TGATTTCCTG 
GCTGCCGAGT TCAAGGATGT GGGTCCACCA ACAGGACTGA CGCTGCCCAA ACGCCCACCA 
AAGGGTGGAG GAGCCAAACC CCCCACCAGA GGCATGTTCA CTGGGGGAAC CAGAGGAAGA 
GGCTTCCAGA GTCACACACG CTTCTTCACT CCACCGGCCC CCAAGAGTGT GCTGCTCGCT 
GGTAACTATC CTCGTCGGGA AGGAGGTCGT GGATCCACTT GGAGCACACC AGTGACTGCT 
GTTACTCACA GAGGGACTTA CAGTGAGCCT CGTGGAGGAC AGAGCAACTT CACTCGACCA 
CTTCCTTCCA GACAGCCACC TACAGGTGCT TACAGACTGG CTCCTCGAGA CAGGGCCACC 
AGAGGCAGAG GGACGGGCCT GTCCTGGCTG AGCGCTGGAG TGAGTGTTGG AGGAGGCGGT 
GGGGTGAGCG GCGGTGGACG CGGCCAAGGC AAGTTCAGCA GCGGAGGCAG TGGAGGGGGA 
CGAGGCCGAC ACGTCCGCTC ATTCACTCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158091.1
CDS	31..5583
Translation	MAGDSAMELLFLDTFKHQNAELTNVDVVRFPYGVLITEVRVIPPGIKAHSNLPDSRAFGETAPHAFQLDLFFNNVSKPNSPVFDRLGSLEYDENKSIIFRPNGKINTDGLVLRGWYTCLTLAVYGTAERPHSHERDSPPPPPPPPPQQQQIGSKRLIKLEWENEDQFNGSPPRPQPRGPRTPPGPPPPDDDDEEAMPAPGQCPVKVENTEHPGDDYLEPVSPERGSLPADETYSDAEQEDDEGPADEEEDDVQSEGTEGDEDDEEDEVGDDVTDEDGEGDDGYEQISSDEEDLESGAFKLPAFDLDYTPEDLASLPTVQYDPYERELRPLQHFTPPHITRYEAELKRLKTVDIEDSSSSWAESAAKLSELLEAWGEAQGAERGAGWVTALEEVPAFLVKGMSFLSIKDPDGQSEKLKQLVDWSCEALSLDVALAQPIALNLRQLKAGTKLVSALADCGAQAVQDLLEKGIVGRLLGLLFAEHVSSTLKLNVLRSLDSIISEPQGIEAMLRDRHHHDDDGTSGYQRLLDLFLLDQTVRVATAGAAILHKGHLYEVLTDLQKTASVWAERHPTVGEVAEREGSESEAGERDRESTEREGEERERETQEAERESPMDIDTLLESASLSEADLERLQGLLEELLHLLETAPHCMVQPPGKAFPTNVRITGPPERDDPYPTLYRYMHACHLLESLGVVLSYPATCAHIGVLQAVRELMRFLSQSQQGLLFLLSQHETINLLLRLLTPLTHLTPPSHLAPLTPLSEVEGEEAAAGGEGLMDDGFWVWLMQTLHALQGIAELNGAELEDGDNPDVLATLHSLYLISFSSTGRNAVAHTFSLDNNLSCLINLIEFHAKDGQGDGKARKSVTYNYACMLVLLVIQTSSDLRMMEHYAAPLLLVCKADENNAKLQELSKWLEPLEKLHFDISGIPMLIDYIKQNLENLMTADGVGLVTALRVLCHIACPPATVPGQQKDLKWNQAIIALFSAEGMDTFIKVLQKLTSLLLLPWRLHGPMGPTAQRLMMLSVASCSLRLIRAMLTELLCGGTCEFRDVRVPSVCVTLHKILCSTPSTGRLDAEELRIQGDVTEILLTFTHAVSEQETGSEETVAGNSWSLMLKEVLNSILAAPENIYSSLSLLSELLPLPLPMQTTQALSVQDIGVAMNTRKLWSVHLHAQARVLQEVLRCACVSSCPPLFSMLRRVCVQLCDLAAPTATLVMRTLLEQLQEEMQQEAAGSRLIRVLALLDSLASQRACKVAMLALLSGSARSDTRSEGKVEDKLSDILPSLLAMLVLPADVGLSAQQNAELMASILQSLCDQDVSLVVSGSGEASVSEAELLANALPTKEMMTSICEGMLDVLGNTHSSYTLQLTCLRTMMFLTEHDYGLYHLKSSLKKHTTAISSLIRREITSFNKEFAELLSALFDFLRQILNSEAMFLRSAFYRHHHADEDMDSRSLALSVSDMKQLLQWKEDSGEHLLCELEKHITKLCKEDESLEALLENVVGLRQIIESSGDAQTNAEPETEPSLPAPEPLQTQFNHRTVYVLSDVLDEQLKALWFSPFQPDEMETEVDMVKVDLVALAQQCCTELDLKAELERSFLSEPSSPGHSKPSKGFRLGKHKHETFITSRYTSGRSDYVEPAKRAHIMAAPRGRGRGSFGQLSRPHDIFRQRKQNTSRPPSMHVDDFLAAEFKDVGPPTGLTLPKRPPKGGGAKPPTRGMFTGGTRGRGFQSHTRFFTPPAPKSVLLAGNYPRREGGRGSTWSTPVTAVTHRGTYSEPRGGQSNFTRPLPSRQPPTGAYRLAPRDRATRGRGTGLSWLSAGVSVGGGGGVSGGGRGQGKFSSGGSGGGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_005158034.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158034.4

ORF Insert Sequence:

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGTTT CTCAGATCTG CATTCTATAG GCATCATCAC 
GCAGATGAAG ACATGGACAG CCGCTCTTTG GCTCTTAGCG TGAGTGACAT GAAGCAGCTG 
TTGCAGTGGA AGGAGGACTC GGGAGAGCAT CTGCTATGTG AACTGGAGAA ACATATTACG 
AAGCTCTGTA AGGAGGACGA GTCTTTGGAG GCTCTGCTTG AGAATGTAGT TGGACTGCGA 
CAGATTATTG AAAGTTCTGG AGACGCACAG ACTAATGCTG AGCCTGAGAC TGAGCCCTCT 
CTACCTGCGC CCGAGCCCCT TCAGACACAG TTCAACCACA GGACAGTGTA TGTACTTTCT 
GATGTCCTGG ATGAGCAGTT GAAGGCCCTG TGGTTTTCTC CATTCCAGCC AGATGAGATG 
GAGACAGAAG TAGACATGGT GAAAGTAGAC CTGGTTGCGC TGGCCCAGCA GTGTTGTACA 
GAATTAGACC TGAAGGCAGA GTTAGAGCGC TCATTCCTCA GTGAACCTTC TTCTCCAGGA 
CACAGCAAAC CAAGCAAAGG CTTCAGACTG GGCAAACACA AACACGAGAC CTTTATTACA 
TCCAGGTACA CAAGCGGAAG GTCTGACTAT GTGGAGCCAG CGAAGCGAGC TCATATCATG 
GCGGCCCCTC GGGGTCGTGG CCGCGGTTCG TTTGGCCAGC TCTCTCGACC ACATGACATT 
TTCCGCCAGC GCAAGCAGAA CACCAGTCGG CCTCCTTCCA TGCATGTAGA TGATTTCCTG 
GCTGCCGAGT TCAAGGATGT GGGTCCACCA ACAGGACTGA CGCTGCCCAA ACGCCCACCA 
AAGGGTGGAG GAGCCAAACC CCCCACCAGA GGCATGTTCA CTGGGGGAAC CAGAGGAAGA 
GGCTTCCAGA GTCACACACG CTTCTTCACT CCACCGGCCC CCAAGAGTGT GCTGCTCGCT 
GGTAACTATC CTCGTCGGGA AGGAGGTCGT GGATCCACTT GGAGCACACC AGTGACTGCT 
GTTACTCACA GAGGGACTTA CAGTGAGCCT CGTGGAGGAC AGAGCAACTT CACTCGACCA 
CTTCCTTCCA GACAGCCACC TACAGGTGCT TACAGACTGG CTCCTCGAGA CAGGGCCACC 
AGAGGCAGAG GGACGGGCCT GTCCTGGCTG AGCGCTGGAG TGAGTGTTGG AGGAGGCGGT 
GGGGTGAGCG GCGGTGGACG CGGCCAAGGC AAGTTCAGCA GCGGAGGCAG TGGAGGGGGA 
CGAGGCCGAC ACGTCCGCTC ATTCACTCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-79l20.4 ( XM_005158035.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158092.1 Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21522
Clone ID Related Accession (Same CDS sequence)	XM_005158035.4 , XM_005158035.3
Accession Version	XM_005158035.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein virilizer homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158035.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGTTT CTCAGATCTG CATTCTATAG GCATCATCAC 
GCAGATGAAG ACATGGACAG CCGCTCTTTG GCTCTTAGCG TGAGTGACAT GAAGCAGCTG 
TTGCAGTGGA AGGAGGACTC GGGAGAGCAT CTGCTATGTG AACTGGAGAA ACATATTACG 
AAGCTCTGTA AGGAGGACGA GTCTTTGGAG GCTCTGCTTG AGAATGTAGT TGGACTGCGA 
CAGATTATTG AAAGTTCTGG AGACGCACAG ACTAATGCTG AGCCTGAGAC TGAGCCCTCT 
CTACCTGCGC CCGAGCCCCT TCAGACACAG TTCAACCACA GGACAGTGTA TGTACTTTCT 
GATGTCCTGG ATGAGCAGTT GAAGGCCCTG TGGTTTTCTC CATTCCAGCC AGATGAGATG 
GAGACAGAAG TAGACATGGT GAAAGTAGAC CTGGTTGCGC TGGCCCAGCA GTGTTGTACA 
GAATTAGACC TGAAGGCAGA GTTAGAGCGC TCATTCCTCA GTGAACCTTC TTCTCCAGGA 
CACAGCAAAC CAAGCAAAGG CTTCAGACTG GGCAAACACA AACACGAGAC CTTTATTACA 
TCCAGCGGAA GGTCTGACTA TGTGGAGCCA GCGAAGCGAG CTCATATCAT GGCGGCCCCT 
CGGGGTCGTG GCCGCGGTTC GTTTGGCCAG CTCTCTCGAC CACATGACAT TTTCCGCCAG 
CGCAAGCAGA ACACCAGTCG GCCTCCTTCC ATGCATGTAG ATGATTTCCT GGCTGCCGAG 
TTCAAGGATG TGGGTCCACC AACAGGACTG ACGCTGCCCA AACGCCCACC AAAGGGTGGA 
GGAGCCAAAC CCCCCACCAG AGGCATGTTC ACTGGGGGAA CCAGAGGAAG AGGCTTCCAG 
AGTCACACAC GCTTCTTCAC TCCACCGGCC CCCAAGAGTG TGCTGCTCGC TGGTAACTAT 
CCTCGTCGGG AAGGAGGTCG TGGATCCACT TGGAGCACAC CAGTGACTGC TGTTACTCAC 
AGAGGGACTT ACAGTGAGCC TCGTGGAGGA CAGAGCAACT TCACTCGACC ACTTCCTTCC 
AGACAGCCAC CTACAGGTGC TTACAGACTG GCTCCTCGAG ACAGGGCCAC CAGAGGCAGA 
GGGACGGGCC TGTCCTGGCT GAGCGCTGGA GTGAGTGTTG GAGGAGGCGG TGGGGTGAGC 
GGCGGTGGAC GCGGCCAAGG CAAGTTCAGC AGCGGAGGCA GTGGAGGGGG ACGAGGCCGA 
CACGTCCGCT CATTCACTCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158092.1
CDS	31..5574
Translation	MAGDSAMELLFLDTFKHQNAELTNVDVVRFPYGVLITEVRVIPPGIKAHSNLPDSRAFGETAPHAFQLDLFFNNVSKPNSPVFDRLGSLEYDENKSIIFRPNGKINTDGLVLRGWYTCLTLAVYGTAERPHSHERDSPPPPPPPPPQQQQIGSKRLIKLEWENEDQFNGSPPRPQPRGPRTPPGPPPPDDDDEEAMPAPGQCPVKVENTEHPGDDYLEPVSPERGSLPADETYSDAEQEDDEGPADEEEDDVQSEGTEGDEDDEEDEVGDDVTDEDGEGDDGYEQISSDEEDLESGAFKLPAFDLDYTPEDLASLPTVQYDPYERELRPLQHFTPPHITRYEAELKRLKTVDIEDSSSSWAESAAKLSELLEAWGEAQGAERGAGWVTALEEVPAFLVKGMSFLSIKDPDGQSEKLKQLVDWSCEALSLDVALAQPIALNLRQLKAGTKLVSALADCGAQAVQDLLEKGIVGRLLGLLFAEHVSSTLKLNVLRSLDSIISEPQGIEAMLRDRHHHDDDGTSGYQRLLDLFLLDQTVRVATAGAAILHKGHLYEVLTDLQKTASVWAERHPTVGEVAEREGSESEAGERDRESTEREGEERERETQEAERESPMDIDTLLESASLSEADLERLQGLLEELLHLLETAPHCMVQPPGKAFPTNVRITGPPERDDPYPTLYRYMHACHLLESLGVVLSYPATCAHIGVLQAVRELMRFLSQSQQGLLFLLSQHETINLLLRLLTPLTHLTPPSHLAPLTPLSEVEGEEAAAGGEGLMDDGFWVWLMQTLHALQGIAELNGAELEDGDNPDVLATLHSLYLISFSSTGRNAVAHTFSLDNNLSCLINLIEFHAKDGQGDGKARKSVTYNYACMLVLLVIQTSSDLRMMEHYAAPLLLVCKADENNAKLQELSKWLEPLEKLHFDISGIPMLIDYIKQNLENLMTADGVGLVTALRVLCHIACPPATVPGQQKDLKWNQAIIALFSAEGMDTFIKVLQKLTSLLLLPWRLHGPMGPTAQRLMMLSVASCSLRLIRAMLTELLCGGTCEFRDVRVPSVCVTLHKILCSTPSTGRLDAEELRIQGDVTEILLTFTHAVSEQETGSEETVAGNSWSLMLKEVLNSILAAPENIYSSLSLLSELLPLPLPMQTTQALSVQDIGVAMNTRKLWSVHLHAQARVLQEVLRCACVSSCPPLFSMLRRVCVQLCDLAAPTATLVMRTLLEQLQEEMQQEAAGSRLIRVLALLDSLASQRACKVAMLALLSGSARSDTRSEGKVEDKLSDILPSLLAMLVLPADVGLSAQQNAELMASILQSLCDQDVSLVVSGSGEASVSEAELLANALPTKEMMTSICEGMLDVLGNTHSSYTLQLTCLRTMMFLTEHDYGLYHLKSSLKKHTTAISSLIRREITSFNKEFAELLSALFDFLRQILNSEAMFLRSAFYRHHHADEDMDSRSLALSVSDMKQLLQWKEDSGEHLLCELEKHITKLCKEDESLEALLENVVGLRQIIESSGDAQTNAEPETEPSLPAPEPLQTQFNHRTVYVLSDVLDEQLKALWFSPFQPDEMETEVDMVKVDLVALAQQCCTELDLKAELERSFLSEPSSPGHSKPSKGFRLGKHKHETFITSSGRSDYVEPAKRAHIMAAPRGRGRGSFGQLSRPHDIFRQRKQNTSRPPSMHVDDFLAAEFKDVGPPTGLTLPKRPPKGGGAKPPTRGMFTGGTRGRGFQSHTRFFTPPAPKSVLLAGNYPRREGGRGSTWSTPVTAVTHRGTYSEPRGGQSNFTRPLPSRQPPTGAYRLAPRDRATRGRGTGLSWLSAGVSVGGGGGVSGGGRGQGKFSSGGSGGGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_005158035.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158035.4

ORF Insert Sequence:

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGTTT CTCAGATCTG CATTCTATAG GCATCATCAC 
GCAGATGAAG ACATGGACAG CCGCTCTTTG GCTCTTAGCG TGAGTGACAT GAAGCAGCTG 
TTGCAGTGGA AGGAGGACTC GGGAGAGCAT CTGCTATGTG AACTGGAGAA ACATATTACG 
AAGCTCTGTA AGGAGGACGA GTCTTTGGAG GCTCTGCTTG AGAATGTAGT TGGACTGCGA 
CAGATTATTG AAAGTTCTGG AGACGCACAG ACTAATGCTG AGCCTGAGAC TGAGCCCTCT 
CTACCTGCGC CCGAGCCCCT TCAGACACAG TTCAACCACA GGACAGTGTA TGTACTTTCT 
GATGTCCTGG ATGAGCAGTT GAAGGCCCTG TGGTTTTCTC CATTCCAGCC AGATGAGATG 
GAGACAGAAG TAGACATGGT GAAAGTAGAC CTGGTTGCGC TGGCCCAGCA GTGTTGTACA 
GAATTAGACC TGAAGGCAGA GTTAGAGCGC TCATTCCTCA GTGAACCTTC TTCTCCAGGA 
CACAGCAAAC CAAGCAAAGG CTTCAGACTG GGCAAACACA AACACGAGAC CTTTATTACA 
TCCAGCGGAA GGTCTGACTA TGTGGAGCCA GCGAAGCGAG CTCATATCAT GGCGGCCCCT 
CGGGGTCGTG GCCGCGGTTC GTTTGGCCAG CTCTCTCGAC CACATGACAT TTTCCGCCAG 
CGCAAGCAGA ACACCAGTCG GCCTCCTTCC ATGCATGTAG ATGATTTCCT GGCTGCCGAG 
TTCAAGGATG TGGGTCCACC AACAGGACTG ACGCTGCCCA AACGCCCACC AAAGGGTGGA 
GGAGCCAAAC CCCCCACCAG AGGCATGTTC ACTGGGGGAA CCAGAGGAAG AGGCTTCCAG 
AGTCACACAC GCTTCTTCAC TCCACCGGCC CCCAAGAGTG TGCTGCTCGC TGGTAACTAT 
CCTCGTCGGG AAGGAGGTCG TGGATCCACT TGGAGCACAC CAGTGACTGC TGTTACTCAC 
AGAGGGACTT ACAGTGAGCC TCGTGGAGGA CAGAGCAACT TCACTCGACC ACTTCCTTCC 
AGACAGCCAC CTACAGGTGC TTACAGACTG GCTCCTCGAG ACAGGGCCAC CAGAGGCAGA 
GGGACGGGCC TGTCCTGGCT GAGCGCTGGA GTGAGTGTTG GAGGAGGCGG TGGGGTGAGC 
GGCGGTGGAC GCGGCCAAGG CAAGTTCAGC AGCGGAGGCA GTGGAGGGGG ACGAGGCCGA 
CACGTCCGCT CATTCACTCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-79l20.4 ( XM_005158035.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158092.1 Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21522
Clone ID Related Accession (Same CDS sequence)	XM_005158035.4 , XM_005158035.3
Accession Version	XM_005158035.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein virilizer homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGTTT CTCAGATCTG CATTCTATAG GCATCATCAC 
GCAGATGAAG ACATGGACAG CCGCTCTTTG GCTCTTAGCG TGAGTGACAT GAAGCAGCTG 
TTGCAGTGGA AGGAGGACTC GGGAGAGCAT CTGCTATGTG AACTGGAGAA ACATATTACG 
AAGCTCTGTA AGGAGGACGA GTCTTTGGAG GCTCTGCTTG AGAATGTAGT TGGACTGCGA 
CAGATTATTG AAAGTTCTGG AGACGCACAG ACTAATGCTG AGCCTGAGAC TGAGCCCTCT 
CTACCTGCGC CCGAGCCCCT TCAGACACAG TTCAACCACA GGACAGTGTA TGTACTTTCT 
GATGTCCTGG ATGAGCAGTT GAAGGCCCTG TGGTTTTCTC CATTCCAGCC AGATGAGATG 
GAGACAGAAG TAGACATGGT GAAAGTAGAC CTGGTTGCGC TGGCCCAGCA GTGTTGTACA 
GAATTAGACC TGAAGGCAGA GTTAGAGCGC TCATTCCTCA GTGAACCTTC TTCTCCAGGA 
CACAGCAAAC CAAGCAAAGG CTTCAGACTG GGCAAACACA AACACGAGAC CTTTATTACA 
TCCAGCGGAA GGTCTGACTA TGTGGAGCCA GCGAAGCGAG CTCATATCAT GGCGGCCCCT 
CGGGGTCGTG GCCGCGGTTC GTTTGGCCAG CTCTCTCGAC CACATGACAT TTTCCGCCAG 
CGCAAGCAGA ACACCAGTCG GCCTCCTTCC ATGCATGTAG ATGATTTCCT GGCTGCCGAG 
TTCAAGGATG TGGGTCCACC AACAGGACTG ACGCTGCCCA AACGCCCACC AAAGGGTGGA 
GGAGCCAAAC CCCCCACCAG AGGCATGTTC ACTGGGGGAA CCAGAGGAAG AGGCTTCCAG 
AGTCACACAC GCTTCTTCAC TCCACCGGCC CCCAAGAGTG TGCTGCTCGC TGGTAACTAT 
CCTCGTCGGG AAGGAGGTCG TGGATCCACT TGGAGCACAC CAGTGACTGC TGTTACTCAC 
AGAGGGACTT ACAGTGAGCC TCGTGGAGGA CAGAGCAACT TCACTCGACC ACTTCCTTCC 
AGACAGCCAC CTACAGGTGC TTACAGACTG GCTCCTCGAG ACAGGGCCAC CAGAGGCAGA 
GGGACGGGCC TGTCCTGGCT GAGCGCTGGA GTGAGTGTTG GAGGAGGCGG TGGGGTGAGC 
GGCGGTGGAC GCGGCCAAGG CAAGTTCAGC AGCGGAGGCA GTGGAGGGGG ACGAGGCCGA 
CACGTCCGCT CATTCACTCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158092.1
CDS	20..5563
Translation	MAGDSAMELLFLDTFKHQNAELTNVDVVRFPYGVLITEVRVIPPGIKAHSNLPDSRAFGETAPHAFQLDLFFNNVSKPNSPVFDRLGSLEYDENKSIIFRPNGKINTDGLVLRGWYTCLTLAVYGTAERPHSHERDSPPPPPPPPPQQQQIGSKRLIKLEWENEDQFNGSPPRPQPRGPRTPPGPPPPDDDDEEAMPAPGQCPVKVENTEHPGDDYLEPVSPERGSLPADETYSDAEQEDDEGPADEEEDDVQSEGTEGDEDDEEDEVGDDVTDEDGEGDDGYEQISSDEEDLESGAFKLPAFDLDYTPEDLASLPTVQYDPYERELRPLQHFTPPHITRYEAELKRLKTVDIEDSSSSWAESAAKLSELLEAWGEAQGAERGAGWVTALEEVPAFLVKGMSFLSIKDPDGQSEKLKQLVDWSCEALSLDVALAQPIALNLRQLKAGTKLVSALADCGAQAVQDLLEKGIVGRLLGLLFAEHVSSTLKLNVLRSLDSIISEPQGIEAMLRDRHHHDDDGTSGYQRLLDLFLLDQTVRVATAGAAILHKGHLYEVLTDLQKTASVWAERHPTVGEVAEREGSESEAGERDRESTEREGEERERETQEAERESPMDIDTLLESASLSEADLERLQGLLEELLHLLETAPHCMVQPPGKAFPTNVRITGPPERDDPYPTLYRYMHACHLLESLGVVLSYPATCAHIGVLQAVRELMRFLSQSQQGLLFLLSQHETINLLLRLLTPLTHLTPPSHLAPLTPLSEVEGEEAAAGGEGLMDDGFWVWLMQTLHALQGIAELNGAELEDGDNPDVLATLHSLYLISFSSTGRNAVAHTFSLDNNLSCLINLIEFHAKDGQGDGKARKSVTYNYACMLVLLVIQTSSDLRMMEHYAAPLLLVCKADENNAKLQELSKWLEPLEKLHFDISGIPMLIDYIKQNLENLMTADGVGLVTALRVLCHIACPPATVPGQQKDLKWNQAIIALFSAEGMDTFIKVLQKLTSLLLLPWRLHGPMGPTAQRLMMLSVASCSLRLIRAMLTELLCGGTCEFRDVRVPSVCVTLHKILCSTPSTGRLDAEELRIQGDVTEILLTFTHAVSEQETGSEETVAGNSWSLMLKEVLNSILAAPENIYSSLSLLSELLPLPLPMQTTQALSVQDIGVAMNTRKLWSVHLHAQARVLQEVLRCACVSSCPPLFSMLRRVCVQLCDLAAPTATLVMRTLLEQLQEEMQQEAAGSRLIRVLALLDSLASQRACKVAMLALLSGSARSDTRSEGKVEDKLSDILPSLLAMLVLPADVGLSAQQNAELMASILQSLCDQDVSLVVSGSGEASVSEAELLANALPTKEMMTSICEGMLDVLGNTHSSYTLQLTCLRTMMFLTEHDYGLYHLKSSLKKHTTAISSLIRREITSFNKEFAELLSALFDFLRQILNSEAMFLRSAFYRHHHADEDMDSRSLALSVSDMKQLLQWKEDSGEHLLCELEKHITKLCKEDESLEALLENVVGLRQIIESSGDAQTNAEPETEPSLPAPEPLQTQFNHRTVYVLSDVLDEQLKALWFSPFQPDEMETEVDMVKVDLVALAQQCCTELDLKAELERSFLSEPSSPGHSKPSKGFRLGKHKHETFITSSGRSDYVEPAKRAHIMAAPRGRGRGSFGQLSRPHDIFRQRKQNTSRPPSMHVDDFLAAEFKDVGPPTGLTLPKRPPKGGGAKPPTRGMFTGGTRGRGFQSHTRFFTPPAPKSVLLAGNYPRREGGRGSTWSTPVTAVTHRGTYSEPRGGQSNFTRPLPSRQPPTGAYRLAPRDRATRGRGTGLSWLSAGVSVGGGGGVSGGGRGQGKFSSGGSGGGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_005158035.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158035.3

ORF Insert Sequence:

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGTTT CTCAGATCTG CATTCTATAG GCATCATCAC 
GCAGATGAAG ACATGGACAG CCGCTCTTTG GCTCTTAGCG TGAGTGACAT GAAGCAGCTG 
TTGCAGTGGA AGGAGGACTC GGGAGAGCAT CTGCTATGTG AACTGGAGAA ACATATTACG 
AAGCTCTGTA AGGAGGACGA GTCTTTGGAG GCTCTGCTTG AGAATGTAGT TGGACTGCGA 
CAGATTATTG AAAGTTCTGG AGACGCACAG ACTAATGCTG AGCCTGAGAC TGAGCCCTCT 
CTACCTGCGC CCGAGCCCCT TCAGACACAG TTCAACCACA GGACAGTGTA TGTACTTTCT 
GATGTCCTGG ATGAGCAGTT GAAGGCCCTG TGGTTTTCTC CATTCCAGCC AGATGAGATG 
GAGACAGAAG TAGACATGGT GAAAGTAGAC CTGGTTGCGC TGGCCCAGCA GTGTTGTACA 
GAATTAGACC TGAAGGCAGA GTTAGAGCGC TCATTCCTCA GTGAACCTTC TTCTCCAGGA 
CACAGCAAAC CAAGCAAAGG CTTCAGACTG GGCAAACACA AACACGAGAC CTTTATTACA 
TCCAGCGGAA GGTCTGACTA TGTGGAGCCA GCGAAGCGAG CTCATATCAT GGCGGCCCCT 
CGGGGTCGTG GCCGCGGTTC GTTTGGCCAG CTCTCTCGAC CACATGACAT TTTCCGCCAG 
CGCAAGCAGA ACACCAGTCG GCCTCCTTCC ATGCATGTAG ATGATTTCCT GGCTGCCGAG 
TTCAAGGATG TGGGTCCACC AACAGGACTG ACGCTGCCCA AACGCCCACC AAAGGGTGGA 
GGAGCCAAAC CCCCCACCAG AGGCATGTTC ACTGGGGGAA CCAGAGGAAG AGGCTTCCAG 
AGTCACACAC GCTTCTTCAC TCCACCGGCC CCCAAGAGTG TGCTGCTCGC TGGTAACTAT 
CCTCGTCGGG AAGGAGGTCG TGGATCCACT TGGAGCACAC CAGTGACTGC TGTTACTCAC 
AGAGGGACTT ACAGTGAGCC TCGTGGAGGA CAGAGCAACT TCACTCGACC ACTTCCTTCC 
AGACAGCCAC CTACAGGTGC TTACAGACTG GCTCCTCGAG ACAGGGCCAC CAGAGGCAGA 
GGGACGGGCC TGTCCTGGCT GAGCGCTGGA GTGAGTGTTG GAGGAGGCGG TGGGGTGAGC 
GGCGGTGGAC GCGGCCAAGG CAAGTTCAGC AGCGGAGGCA GTGGAGGGGG ACGAGGCCGA 
CACGTCCGCT CATTCACTCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-79l20.4 ( XM_683656.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_688748.4 Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X3, mRNA.

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CloneID	ODa21523
Clone ID Related Accession (Same CDS sequence)	XM_683656.7 , XM_683656.8
Accession Version	XM_683656.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein virilizer homolog isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_683656.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGGAT GAAGACATGG ACAGCCGCTC TTTGGCTCTT 
AGCGTGAGTG ACATGAAGCA GCTGTTGCAG TGGAAGGAGG ACTCGGGAGA GCATCTGCTA 
TGTGAACTGG AGAAACATAT TACGAAGCTC TGTAAGGAGG ACGAGTCTTT GGAGGCTCTG 
CTTGAGAATG TAGTTGGACT GCGACAGATT ATTGAAAGTT CTGGAGACGC ACAGACTAAT 
GCTGAGCCTG AGACTGAGCC CTCTCTACCT GCGCCCGAGC CCCTTCAGAC ACAGTTCAAC 
CACAGGACAG TGTATGTACT TTCTGATGTC CTGGATGAGC AGTTGAAGGC CCTGTGGTTT 
TCTCCATTCC AGCCAGATGA GATGGAGACA GAAGTAGACA TGGTGAAAGT AGACCTGGTT 
GCGCTGGCCC AGCAGTGTTG TACAGAATTA GACCTGAAGG CAGAGTTAGA GCGCTCATTC 
CTCAGTGAAC CTTCTTCTCC AGGACACAGC AAACCAAGCA AAGGCTTCAG ACTGGGCAAA 
CACAAACACG AGACCTTTAT TACATCCAGG TACACAAGCG GAAGGTCTGA CTATGTGGAG 
CCAGCGAAGC GAGCTCATAT CATGGCGGCC CCTCGGGGTC GTGGCCGCGG TTCGTTTGGC 
CAGCTCTCTC GACCACATGA CATTTTCCGC CAGCGCAAGC AGAACACCAG TCGGCCTCCT 
TCCATGCATG TAGATGATTT CCTGGCTGCC GAGTTCAAGG ATGTGGGTCC ACCAACAGGA 
CTGACGCTGC CCAAACGCCC ACCAAAGGGT GGAGGAGCCA AACCCCCCAC CAGAGGCATG 
TTCACTGGGG GAACCAGAGG AAGAGGCTTC CAGAGTCACA CACGCTTCTT CACTCCACCG 
GCCCCCAAGA GTGTGCTGCT CGCTGGTAAC TATCCTCGTC GGGAAGGAGG TCGTGGATCC 
ACTTGGAGCA CACCAGTGAC TGCTGTTACT CACAGAGGGA CTTACAGTGA GCCTCGTGGA 
GGACAGAGCA ACTTCACTCG ACCACTTCCT TCCAGACAGC CACCTACAGG TGCTTACAGA 
CTGGCTCCTC GAGACAGGGC CACCAGAGGC AGAGGGACGG GCCTGTCCTG GCTGAGCGCT 
GGAGTGAGTG TTGGAGGAGG CGGTGGGGTG AGCGGCGGTG GACGCGGCCA AGGCAAGTTC 
AGCAGCGGAG GCAGTGGAGG GGGACGAGGC CGACACGTCC GCTCATTCAC TCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688748.4
CDS	20..5536
Translation	MAGDSAMELLFLDTFKHQNAELTNVDVVRFPYGVLITEVRVIPPGIKAHSNLPDSRAFGETAPHAFQLDLFFNNVSKPNSPVFDRLGSLEYDENKSIIFRPNGKINTDGLVLRGWYTCLTLAVYGTAERPHSHERDSPPPPPPPPPQQQQIGSKRLIKLEWENEDQFNGSPPRPQPRGPRTPPGPPPPDDDDEEAMPAPGQCPVKVENTEHPGDDYLEPVSPERGSLPADETYSDAEQEDDEGPADEEEDDVQSEGTEGDEDDEEDEVGDDVTDEDGEGDDGYEQISSDEEDLESGAFKLPAFDLDYTPEDLASLPTVQYDPYERELRPLQHFTPPHITRYEAELKRLKTVDIEDSSSSWAESAAKLSELLEAWGEAQGAERGAGWVTALEEVPAFLVKGMSFLSIKDPDGQSEKLKQLVDWSCEALSLDVALAQPIALNLRQLKAGTKLVSALADCGAQAVQDLLEKGIVGRLLGLLFAEHVSSTLKLNVLRSLDSIISEPQGIEAMLRDRHHHDDDGTSGYQRLLDLFLLDQTVRVATAGAAILHKGHLYEVLTDLQKTASVWAERHPTVGEVAEREGSESEAGERDRESTEREGEERERETQEAERESPMDIDTLLESASLSEADLERLQGLLEELLHLLETAPHCMVQPPGKAFPTNVRITGPPERDDPYPTLYRYMHACHLLESLGVVLSYPATCAHIGVLQAVRELMRFLSQSQQGLLFLLSQHETINLLLRLLTPLTHLTPPSHLAPLTPLSEVEGEEAAAGGEGLMDDGFWVWLMQTLHALQGIAELNGAELEDGDNPDVLATLHSLYLISFSSTGRNAVAHTFSLDNNLSCLINLIEFHAKDGQGDGKARKSVTYNYACMLVLLVIQTSSDLRMMEHYAAPLLLVCKADENNAKLQELSKWLEPLEKLHFDISGIPMLIDYIKQNLENLMTADGVGLVTALRVLCHIACPPATVPGQQKDLKWNQAIIALFSAEGMDTFIKVLQKLTSLLLLPWRLHGPMGPTAQRLMMLSVASCSLRLIRAMLTELLCGGTCEFRDVRVPSVCVTLHKILCSTPSTGRLDAEELRIQGDVTEILLTFTHAVSEQETGSEETVAGNSWSLMLKEVLNSILAAPENIYSSLSLLSELLPLPLPMQTTQALSVQDIGVAMNTRKLWSVHLHAQARVLQEVLRCACVSSCPPLFSMLRRVCVQLCDLAAPTATLVMRTLLEQLQEEMQQEAAGSRLIRVLALLDSLASQRACKVAMLALLSGSARSDTRSEGKVEDKLSDILPSLLAMLVLPADVGLSAQQNAELMASILQSLCDQDVSLVVSGSGEASVSEAELLANALPTKEMMTSICEGMLDVLGNTHSSYTLQLTCLRTMMFLTEHDYGLYHLKSSLKKHTTAISSLIRREITSFNKEFAELLSALFDFLRQILNSEAMDEDMDSRSLALSVSDMKQLLQWKEDSGEHLLCELEKHITKLCKEDESLEALLENVVGLRQIIESSGDAQTNAEPETEPSLPAPEPLQTQFNHRTVYVLSDVLDEQLKALWFSPFQPDEMETEVDMVKVDLVALAQQCCTELDLKAELERSFLSEPSSPGHSKPSKGFRLGKHKHETFITSRYTSGRSDYVEPAKRAHIMAAPRGRGRGSFGQLSRPHDIFRQRKQNTSRPPSMHVDDFLAAEFKDVGPPTGLTLPKRPPKGGGAKPPTRGMFTGGTRGRGFQSHTRFFTPPAPKSVLLAGNYPRREGGRGSTWSTPVTAVTHRGTYSEPRGGQSNFTRPLPSRQPPTGAYRLAPRDRATRGRGTGLSWLSAGVSVGGGGGVSGGGRGQGKFSSGGSGGGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_683656.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683656.7

ORF Insert Sequence:

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGGAT GAAGACATGG ACAGCCGCTC TTTGGCTCTT 
AGCGTGAGTG ACATGAAGCA GCTGTTGCAG TGGAAGGAGG ACTCGGGAGA GCATCTGCTA 
TGTGAACTGG AGAAACATAT TACGAAGCTC TGTAAGGAGG ACGAGTCTTT GGAGGCTCTG 
CTTGAGAATG TAGTTGGACT GCGACAGATT ATTGAAAGTT CTGGAGACGC ACAGACTAAT 
GCTGAGCCTG AGACTGAGCC CTCTCTACCT GCGCCCGAGC CCCTTCAGAC ACAGTTCAAC 
CACAGGACAG TGTATGTACT TTCTGATGTC CTGGATGAGC AGTTGAAGGC CCTGTGGTTT 
TCTCCATTCC AGCCAGATGA GATGGAGACA GAAGTAGACA TGGTGAAAGT AGACCTGGTT 
GCGCTGGCCC AGCAGTGTTG TACAGAATTA GACCTGAAGG CAGAGTTAGA GCGCTCATTC 
CTCAGTGAAC CTTCTTCTCC AGGACACAGC AAACCAAGCA AAGGCTTCAG ACTGGGCAAA 
CACAAACACG AGACCTTTAT TACATCCAGG TACACAAGCG GAAGGTCTGA CTATGTGGAG 
CCAGCGAAGC GAGCTCATAT CATGGCGGCC CCTCGGGGTC GTGGCCGCGG TTCGTTTGGC 
CAGCTCTCTC GACCACATGA CATTTTCCGC CAGCGCAAGC AGAACACCAG TCGGCCTCCT 
TCCATGCATG TAGATGATTT CCTGGCTGCC GAGTTCAAGG ATGTGGGTCC ACCAACAGGA 
CTGACGCTGC CCAAACGCCC ACCAAAGGGT GGAGGAGCCA AACCCCCCAC CAGAGGCATG 
TTCACTGGGG GAACCAGAGG AAGAGGCTTC CAGAGTCACA CACGCTTCTT CACTCCACCG 
GCCCCCAAGA GTGTGCTGCT CGCTGGTAAC TATCCTCGTC GGGAAGGAGG TCGTGGATCC 
ACTTGGAGCA CACCAGTGAC TGCTGTTACT CACAGAGGGA CTTACAGTGA GCCTCGTGGA 
GGACAGAGCA ACTTCACTCG ACCACTTCCT TCCAGACAGC CACCTACAGG TGCTTACAGA 
CTGGCTCCTC GAGACAGGGC CACCAGAGGC AGAGGGACGG GCCTGTCCTG GCTGAGCGCT 
GGAGTGAGTG TTGGAGGAGG CGGTGGGGTG AGCGGCGGTG GACGCGGCCA AGGCAAGTTC 
AGCAGCGGAG GCAGTGGAGG GGGACGAGGC CGACACGTCC GCTCATTCAC TCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-79l20.4 ( XM_683656.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_688748.4 Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X3, mRNA.

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CloneID	ODa21523
Clone ID Related Accession (Same CDS sequence)	XM_683656.7 , XM_683656.8
Accession Version	XM_683656.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein virilizer homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_683656.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGGAT GAAGACATGG ACAGCCGCTC TTTGGCTCTT 
AGCGTGAGTG ACATGAAGCA GCTGTTGCAG TGGAAGGAGG ACTCGGGAGA GCATCTGCTA 
TGTGAACTGG AGAAACATAT TACGAAGCTC TGTAAGGAGG ACGAGTCTTT GGAGGCTCTG 
CTTGAGAATG TAGTTGGACT GCGACAGATT ATTGAAAGTT CTGGAGACGC ACAGACTAAT 
GCTGAGCCTG AGACTGAGCC CTCTCTACCT GCGCCCGAGC CCCTTCAGAC ACAGTTCAAC 
CACAGGACAG TGTATGTACT TTCTGATGTC CTGGATGAGC AGTTGAAGGC CCTGTGGTTT 
TCTCCATTCC AGCCAGATGA GATGGAGACA GAAGTAGACA TGGTGAAAGT AGACCTGGTT 
GCGCTGGCCC AGCAGTGTTG TACAGAATTA GACCTGAAGG CAGAGTTAGA GCGCTCATTC 
CTCAGTGAAC CTTCTTCTCC AGGACACAGC AAACCAAGCA AAGGCTTCAG ACTGGGCAAA 
CACAAACACG AGACCTTTAT TACATCCAGG TACACAAGCG GAAGGTCTGA CTATGTGGAG 
CCAGCGAAGC GAGCTCATAT CATGGCGGCC CCTCGGGGTC GTGGCCGCGG TTCGTTTGGC 
CAGCTCTCTC GACCACATGA CATTTTCCGC CAGCGCAAGC AGAACACCAG TCGGCCTCCT 
TCCATGCATG TAGATGATTT CCTGGCTGCC GAGTTCAAGG ATGTGGGTCC ACCAACAGGA 
CTGACGCTGC CCAAACGCCC ACCAAAGGGT GGAGGAGCCA AACCCCCCAC CAGAGGCATG 
TTCACTGGGG GAACCAGAGG AAGAGGCTTC CAGAGTCACA CACGCTTCTT CACTCCACCG 
GCCCCCAAGA GTGTGCTGCT CGCTGGTAAC TATCCTCGTC GGGAAGGAGG TCGTGGATCC 
ACTTGGAGCA CACCAGTGAC TGCTGTTACT CACAGAGGGA CTTACAGTGA GCCTCGTGGA 
GGACAGAGCA ACTTCACTCG ACCACTTCCT TCCAGACAGC CACCTACAGG TGCTTACAGA 
CTGGCTCCTC GAGACAGGGC CACCAGAGGC AGAGGGACGG GCCTGTCCTG GCTGAGCGCT 
GGAGTGAGTG TTGGAGGAGG CGGTGGGGTG AGCGGCGGTG GACGCGGCCA AGGCAAGTTC 
AGCAGCGGAG GCAGTGGAGG GGGACGAGGC CGACACGTCC GCTCATTCAC TCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688748.4
CDS	31..5547
Translation	MAGDSAMELLFLDTFKHQNAELTNVDVVRFPYGVLITEVRVIPPGIKAHSNLPDSRAFGETAPHAFQLDLFFNNVSKPNSPVFDRLGSLEYDENKSIIFRPNGKINTDGLVLRGWYTCLTLAVYGTAERPHSHERDSPPPPPPPPPQQQQIGSKRLIKLEWENEDQFNGSPPRPQPRGPRTPPGPPPPDDDDEEAMPAPGQCPVKVENTEHPGDDYLEPVSPERGSLPADETYSDAEQEDDEGPADEEEDDVQSEGTEGDEDDEEDEVGDDVTDEDGEGDDGYEQISSDEEDLESGAFKLPAFDLDYTPEDLASLPTVQYDPYERELRPLQHFTPPHITRYEAELKRLKTVDIEDSSSSWAESAAKLSELLEAWGEAQGAERGAGWVTALEEVPAFLVKGMSFLSIKDPDGQSEKLKQLVDWSCEALSLDVALAQPIALNLRQLKAGTKLVSALADCGAQAVQDLLEKGIVGRLLGLLFAEHVSSTLKLNVLRSLDSIISEPQGIEAMLRDRHHHDDDGTSGYQRLLDLFLLDQTVRVATAGAAILHKGHLYEVLTDLQKTASVWAERHPTVGEVAEREGSESEAGERDRESTEREGEERERETQEAERESPMDIDTLLESASLSEADLERLQGLLEELLHLLETAPHCMVQPPGKAFPTNVRITGPPERDDPYPTLYRYMHACHLLESLGVVLSYPATCAHIGVLQAVRELMRFLSQSQQGLLFLLSQHETINLLLRLLTPLTHLTPPSHLAPLTPLSEVEGEEAAAGGEGLMDDGFWVWLMQTLHALQGIAELNGAELEDGDNPDVLATLHSLYLISFSSTGRNAVAHTFSLDNNLSCLINLIEFHAKDGQGDGKARKSVTYNYACMLVLLVIQTSSDLRMMEHYAAPLLLVCKADENNAKLQELSKWLEPLEKLHFDISGIPMLIDYIKQNLENLMTADGVGLVTALRVLCHIACPPATVPGQQKDLKWNQAIIALFSAEGMDTFIKVLQKLTSLLLLPWRLHGPMGPTAQRLMMLSVASCSLRLIRAMLTELLCGGTCEFRDVRVPSVCVTLHKILCSTPSTGRLDAEELRIQGDVTEILLTFTHAVSEQETGSEETVAGNSWSLMLKEVLNSILAAPENIYSSLSLLSELLPLPLPMQTTQALSVQDIGVAMNTRKLWSVHLHAQARVLQEVLRCACVSSCPPLFSMLRRVCVQLCDLAAPTATLVMRTLLEQLQEEMQQEAAGSRLIRVLALLDSLASQRACKVAMLALLSGSARSDTRSEGKVEDKLSDILPSLLAMLVLPADVGLSAQQNAELMASILQSLCDQDVSLVVSGSGEASVSEAELLANALPTKEMMTSICEGMLDVLGNTHSSYTLQLTCLRTMMFLTEHDYGLYHLKSSLKKHTTAISSLIRREITSFNKEFAELLSALFDFLRQILNSEAMDEDMDSRSLALSVSDMKQLLQWKEDSGEHLLCELEKHITKLCKEDESLEALLENVVGLRQIIESSGDAQTNAEPETEPSLPAPEPLQTQFNHRTVYVLSDVLDEQLKALWFSPFQPDEMETEVDMVKVDLVALAQQCCTELDLKAELERSFLSEPSSPGHSKPSKGFRLGKHKHETFITSRYTSGRSDYVEPAKRAHIMAAPRGRGRGSFGQLSRPHDIFRQRKQNTSRPPSMHVDDFLAAEFKDVGPPTGLTLPKRPPKGGGAKPPTRGMFTGGTRGRGFQSHTRFFTPPAPKSVLLAGNYPRREGGRGSTWSTPVTAVTHRGTYSEPRGGQSNFTRPLPSRQPPTGAYRLAPRDRATRGRGTGLSWLSAGVSVGGGGGVSGGGRGQGKFSSGGSGGGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_683656.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-79l20.4 (si:ch211-79l20.4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683656.8

ORF Insert Sequence:

ATGGCGGGGG ATTCTGCAAT GGAGCTGCTC TTTTTAGATA CGTTCAAGCA TCAAAATGCA 
GAGCTGACAA ATGTGGATGT GGTGCGGTTT CCTTACGGTG TCTTGATCAC AGAAGTGCGG 
GTGATTCCCC CTGGCATCAA AGCACACAGC AACCTGCCTG ACAGCAGAGC ATTTGGGGAA 
ACTGCCCCTC ACGCATTCCA GTTGGACCTG TTCTTCAACA ATGTGTCCAA ACCCAACTCC 
CCGGTCTTTG ACAGGCTGGG CAGCCTTGAA TATGATGAAA ACAAATCAAT CATCTTCAGG 
CCGAATGGAA AGATAAACAC AGATGGGCTG GTTCTGCGTG GCTGGTACAC ATGCTTGACG 
TTGGCGGTGT ATGGTACAGC CGAACGCCCT CACAGTCATG AGCGGGACTC TCCACCGCCT 
CCTCCACCCC CTCCTCCCCA ACAGCAGCAG ATCGGGTCCA AGAGGCTCAT TAAGCTTGAG 
TGGGAGAATG AAGATCAGTT TAATGGCAGC CCACCCAGAC CACAACCCAG AGGTCCACGG 
ACACCACCAG GACCACCTCC CCCAGATGAT GATGATGAGG AAGCAATGCC TGCACCTGGT 
CAATGTCCAG TGAAGGTGGA GAACACAGAA CATCCGGGTG ATGATTACTT GGAACCCGTT 
TCACCTGAGC GAGGATCTCT TCCTGCTGAT GAGACGTACT CTGATGCAGA GCAGGAAGAC 
GATGAGGGTC CAGCTGATGA AGAGGAGGAT GATGTGCAGT CTGAAGGAAC TGAAGGGGAT 
GAAGATGATG AGGAAGATGA AGTGGGTGAT GATGTGACAG ATGAAGATGG TGAAGGTGAT 
GATGGATATG AGCAGATATC CAGTGATGAG GAGGATTTAG AGAGTGGGGC TTTCAAGCTT 
CCTGCTTTTG ACCTGGACTA TACTCCTGAA GACCTCGCCT CACTACCAAC TGTGCAGTAC 
GACCCATATG AGCGTGAACT CAGACCCCTT CAGCACTTCA CCCCACCACA CATCACACGC 
TATGAGGCAG AGCTAAAACG CCTCAAAACG GTGGACATCG AGGACTCGAG CTCCTCATGG 
GCAGAGTCTG CCGCTAAACT CTCAGAACTC CTGGAGGCCT GGGGGGAGGC GCAGGGGGCG 
GAGAGAGGTG CAGGCTGGGT AACTGCTTTA GAGGAAGTCC CTGCCTTTCT AGTAAAAGGA 
ATGAGCTTCC TGTCTATTAA GGACCCTGAT GGGCAGAGTG AAAAGTTGAA GCAATTGGTC 
GACTGGAGCT GTGAAGCTCT TAGTTTGGAC GTTGCTCTGG CGCAGCCCAT AGCACTGAAC 
CTGCGCCAGC TTAAAGCGGG AACCAAACTG GTCTCTGCCT TGGCTGACTG TGGTGCTCAG 
GCTGTGCAGG ATCTGCTGGA GAAGGGGATT GTTGGCAGGC TCCTAGGCCT GCTGTTTGCA 
GAACACGTTT CATCCACTCT GAAACTCAAC GTGCTGCGCT CCCTCGACAG CATCATCAGC 
GAGCCACAGG GCATCGAGGC TATGCTCAGA GACCGCCACC ATCATGATGA TGATGGCACA 
AGCGGGTATC AGCGTCTGCT CGATCTGTTT CTATTGGACC AGACTGTTCG TGTCGCAACT 
GCAGGTGCAG CCATATTACA CAAAGGACAT TTATATGAGG TTTTGACTGA CCTGCAGAAA 
ACCGCCAGTG TTTGGGCTGA ACGGCATCCT ACTGTAGGGG AGGTGGCTGA GCGCGAGGGC 
AGCGAGAGCG AGGCTGGAGA GAGAGACAGA GAAAGCACTG AGAGGGAAGG GGAGGAGAGA 
GAGAGGGAAA CCCAGGAGGC AGAGCGGGAA AGCCCAATGG ACATCGATAC CTTGTTGGAA 
TCTGCCTCTT TGTCAGAGGC TGACCTGGAG AGGCTGCAGG GTCTCCTGGA GGAACTGCTC 
CACCTGCTGG AAACAGCACC TCACTGTATG GTGCAGCCTC CTGGGAAAGC TTTTCCCACC 
AATGTCCGGA TCACTGGGCC ACCAGAGCGA GATGATCCCT ATCCTACACT CTACAGATAC 
ATGCATGCAT GTCACCTTCT GGAATCTCTG GGAGTTGTCT TGTCATATCC AGCTACATGT 
GCCCACATTG GAGTTCTACA GGCCGTTCGA GAACTGATGC GTTTCTTGTC CCAGTCCCAG 
CAGGGCCTCC TCTTCCTCCT TAGTCAACAC GAAACCATTA ATTTGCTGCT CCGCCTCCTC 
ACGCCCCTTA CCCATCTGAC CCCACCCTCT CATTTGGCTC CACTGACGCC CCTTTCTGAG 
GTTGAGGGGG AAGAAGCTGC AGCAGGTGGA GAGGGGCTGA TGGATGATGG CTTTTGGGTG 
TGGCTTATGC AGACTCTACA TGCACTACAG GGTATTGCTG AATTAAACGG GGCAGAGCTT 
GAAGATGGCG ATAATCCAGA TGTTTTGGCA ACACTTCACA GTCTGTATCT CATTAGCTTT 
AGCAGCACGG GCCGAAATGC TGTTGCACAC ACTTTCAGCT TGGATAACAA CCTCAGCTGT 
CTGATAAACC TGATAGAGTT CCATGCCAAA GATGGCCAAG GGGACGGCAA AGCAAGGAAA 
TCTGTAACGT ATAACTATGC CTGTATGCTT GTACTGCTGG TGATTCAGAC ATCAAGTGAT 
CTGCGGATGA TGGAGCACTA TGCCGCTCCA CTTCTTCTTG TTTGCAAAGC TGATGAAAAC 
AACGCCAAGC TTCAAGAGCT CTCTAAGTGG TTGGAGCCAC TTGAAAAATT ACATTTCGAT 
ATCAGTGGCA TTCCTATGCT TATTGACTAC ATAAAGCAGA ATCTGGAGAA CTTGATGACT 
GCAGATGGTG TGGGTCTTGT TACTGCTCTC AGAGTTCTGT GCCACATTGC CTGCCCACCA 
GCAACAGTGC CAGGCCAGCA GAAAGACTTG AAGTGGAACC AGGCCATCAT AGCTCTCTTC 
AGTGCAGAGG GAATGGACAC TTTTATTAAA GTCCTCCAGA AGCTTACATC ACTGTTATTG 
CTGCCATGGC GACTGCATGG GCCAATGGGG CCCACAGCGC AACGACTCAT GATGCTGTCA 
GTGGCCTCCT GCTCGCTGAG ACTCATCCGA GCCATGCTGA CAGAGCTGCT GTGCGGGGGA 
ACCTGCGAGT TTCGTGACGT GCGTGTGCCC TCCGTCTGTG TCACCCTTCA CAAGATCCTC 
TGCTCCACCC CGTCAACCGG CCGCCTGGAT GCAGAGGAGC TGCGTATCCA AGGAGACGTG 
ACGGAGATCC TGTTGACGTT CACACATGCT GTTAGTGAAC AGGAGACAGG TTCTGAGGAG 
ACCGTGGCTG GCAACAGCTG GTCTCTGATG CTGAAGGAAG TGCTGAACTC TATTCTGGCT 
GCTCCAGAGA ATATCTATAG TAGTCTCAGT CTGCTGTCTG AACTACTTCC TCTTCCTCTC 
CCCATGCAGA CCACACAGGC GCTGTCAGTC CAAGACATCG GGGTTGCGAT GAACACACGA 
AAGCTGTGGA GTGTGCACTT ACACGCTCAA GCTCGAGTGT TACAGGAAGT GCTGAGGTGC 
GCGTGTGTGA GCAGTTGTCC GCCGCTTTTC TCCATGCTCC GCCGTGTGTG TGTTCAGCTG 
TGTGATCTTG CTGCACCCAC AGCCACTCTG GTCATGAGGA CACTGCTGGA GCAGCTGCAG 
GAGGAGATGC AACAGGAAGC AGCTGGCTCG CGTTTAATAA GGGTACTCGC CCTCCTCGAC 
TCCCTGGCAT CTCAACGGGC CTGCAAGGTA GCCATGTTGG CTCTGCTCAG TGGCTCTGCA 
CGCAGTGACA CCAGGAGTGA AGGCAAAGTG GAGGACAAAC TGTCAGACAT TCTTCCCTCG 
CTGCTTGCAA TGCTAGTCCT GCCAGCGGAT GTGGGCCTTT CAGCACAGCA GAACGCTGAA 
CTCATGGCTT CCATCCTTCA GTCCCTGTGT GACCAGGACG TGTCGTTAGT AGTGAGCGGT 
TCCGGTGAAG CGTCAGTGTC GGAGGCGGAG CTACTTGCCA ATGCGCTGCC GACCAAAGAA 
ATGATGACCA GTATTTGTGA GGGGATGCTG GACGTCTTGG GGAACACACA TAGCAGCTAC 
ACACTTCAAC TCACATGTCT GCGAACTATG ATGTTTCTCA CAGAGCACGA CTACGGTCTC 
TATCACCTGA AAAGCTCTTT GAAGAAACAC ACAACGGCCA TTTCCTCTCT AATCCGCAGA 
GAAATTACGT CCTTCAACAA AGAGTTTGCG GAGCTCCTTT CAGCCCTCTT TGACTTTTTG 
AGACAGATTC TGAACAGTGA AGCTATGGAT GAAGACATGG ACAGCCGCTC TTTGGCTCTT 
AGCGTGAGTG ACATGAAGCA GCTGTTGCAG TGGAAGGAGG ACTCGGGAGA GCATCTGCTA 
TGTGAACTGG AGAAACATAT TACGAAGCTC TGTAAGGAGG ACGAGTCTTT GGAGGCTCTG 
CTTGAGAATG TAGTTGGACT GCGACAGATT ATTGAAAGTT CTGGAGACGC ACAGACTAAT 
GCTGAGCCTG AGACTGAGCC CTCTCTACCT GCGCCCGAGC CCCTTCAGAC ACAGTTCAAC 
CACAGGACAG TGTATGTACT TTCTGATGTC CTGGATGAGC AGTTGAAGGC CCTGTGGTTT 
TCTCCATTCC AGCCAGATGA GATGGAGACA GAAGTAGACA TGGTGAAAGT AGACCTGGTT 
GCGCTGGCCC AGCAGTGTTG TACAGAATTA GACCTGAAGG CAGAGTTAGA GCGCTCATTC 
CTCAGTGAAC CTTCTTCTCC AGGACACAGC AAACCAAGCA AAGGCTTCAG ACTGGGCAAA 
CACAAACACG AGACCTTTAT TACATCCAGG TACACAAGCG GAAGGTCTGA CTATGTGGAG 
CCAGCGAAGC GAGCTCATAT CATGGCGGCC CCTCGGGGTC GTGGCCGCGG TTCGTTTGGC 
CAGCTCTCTC GACCACATGA CATTTTCCGC CAGCGCAAGC AGAACACCAG TCGGCCTCCT 
TCCATGCATG TAGATGATTT CCTGGCTGCC GAGTTCAAGG ATGTGGGTCC ACCAACAGGA 
CTGACGCTGC CCAAACGCCC ACCAAAGGGT GGAGGAGCCA AACCCCCCAC CAGAGGCATG 
TTCACTGGGG GAACCAGAGG AAGAGGCTTC CAGAGTCACA CACGCTTCTT CACTCCACCG 
GCCCCCAAGA GTGTGCTGCT CGCTGGTAAC TATCCTCGTC GGGAAGGAGG TCGTGGATCC 
ACTTGGAGCA CACCAGTGAC TGCTGTTACT CACAGAGGGA CTTACAGTGA GCCTCGTGGA 
GGACAGAGCA ACTTCACTCG ACCACTTCCT TCCAGACAGC CACCTACAGG TGCTTACAGA 
CTGGCTCCTC GAGACAGGGC CACCAGAGGC AGAGGGACGG GCCTGTCCTG GCTGAGCGCT 
GGAGTGAGTG TTGGAGGAGG CGGTGGGGTG AGCGGCGGTG GACGCGGCCA AGGCAAGTTC 
AGCAGCGGAG GCAGTGGAGG GGGACGAGGC CGACACGTCC GCTCATTCAC TCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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si:ch211-79l20.4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

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FAX

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