GRIN2BB cDNA ORF clone, Danio rerio(Zebrafish)

The following GRIN2BB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRIN2BB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa53707 XM_017355012.2
Latest version!
Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa53708 XM_017355013.2
Latest version!
Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa34765 NM_001128337.2
Latest version!
Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa34765 XM_017355011.2
Latest version!
Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa34765 XM_017355008.2
Latest version!
Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
ODa53708 XM_017355013.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa53707 XM_017355012.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa34765 XM_017355008.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa34765 XM_017355011.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa53707
    Clone ID Related Accession (Same CDS sequence) XM_017355012.1 , XM_017355012.2
    Accession Version XM_017355012.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGAT AAGGTGAACC AGAACCCATT CATCCCAACC
    TTCGGCGACG ACCAGTGTCT ATTACACGGT GCCAAATCTT ACTACATCAA AAAGCAGCAG
    GCACAGCCGC AGCAGCAACA GACGCCGCAG CAGCAGCAGC AACAGCTCCT CAACAACAGT
    CGGGCTGACT TCCGGGGCTC CATGGGGGTG ACTTCCTATT TGCCAGCCTC TGCAACTTCT
    GGGGTGCTTT CCAATGTGGC CCCTCGGTTC CCCAAGGAGT TATGTCTAGG TGGGCCTCTT
    GGCAACCACC ACGGAGGAGG ACCTAGCAAC AACAAGCTGC TGTCAGCAAG GGATGGGTTA
    GGAATGGGTC AAGGACAGAG ACCGTTTAAT GGCTCGAGCA ATGGACATGT TTACGAAAAG
    CTTTCCAGTA TAGAATCTGA TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210501.1
    CDS1425..5891
    Translation

    Target ORF information:

    RefSeq Version XM_017355012.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGAT AAGGTGAACC AGAACCCATT CATCCCAACC
    TTCGGCGACG ACCAGTGTCT ATTACACGGT GCCAAATCTT ACTACATCAA AAAGCAGCAG
    GCACAGCCGC AGCAGCAACA GACGCCGCAG CAGCAGCAGC AACAGCTCCT CAACAACAGT
    CGGGCTGACT TCCGGGGCTC CATGGGGGTG ACTTCCTATT TGCCAGCCTC TGCAACTTCT
    GGGGTGCTTT CCAATGTGGC CCCTCGGTTC CCCAAGGAGT TATGTCTAGG TGGGCCTCTT
    GGCAACCACC ACGGAGGAGG ACCTAGCAAC AACAAGCTGC TGTCAGCAAG GGATGGGTTA
    GGAATGGGTC AAGGACAGAG ACCGTTTAAT GGCTCGAGCA ATGGACATGT TTACGAAAAG
    CTTTCCAGTA TAGAATCTGA TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53707
    Clone ID Related Accession (Same CDS sequence) XM_017355012.1 , XM_017355012.2
    Accession Version XM_017355012.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGAT AAGGTGAACC AGAACCCATT CATCCCAACC
    TTCGGCGACG ACCAGTGTCT ATTACACGGT GCCAAATCTT ACTACATCAA AAAGCAGCAG
    GCACAGCCGC AGCAGCAACA GACGCCGCAG CAGCAGCAGC AACAGCTCCT CAACAACAGT
    CGGGCTGACT TCCGGGGCTC CATGGGGGTG ACTTCCTATT TGCCAGCCTC TGCAACTTCT
    GGGGTGCTTT CCAATGTGGC CCCTCGGTTC CCCAAGGAGT TATGTCTAGG TGGGCCTCTT
    GGCAACCACC ACGGAGGAGG ACCTAGCAAC AACAAGCTGC TGTCAGCAAG GGATGGGTTA
    GGAATGGGTC AAGGACAGAG ACCGTTTAAT GGCTCGAGCA ATGGACATGT TTACGAAAAG
    CTTTCCAGTA TAGAATCTGA TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210501.1
    CDS1393..5859
    Translation

    Target ORF information:

    RefSeq Version XM_017355012.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355012.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGAT AAGGTGAACC AGAACCCATT CATCCCAACC
    TTCGGCGACG ACCAGTGTCT ATTACACGGT GCCAAATCTT ACTACATCAA AAAGCAGCAG
    GCACAGCCGC AGCAGCAACA GACGCCGCAG CAGCAGCAGC AACAGCTCCT CAACAACAGT
    CGGGCTGACT TCCGGGGCTC CATGGGGGTG ACTTCCTATT TGCCAGCCTC TGCAACTTCT
    GGGGTGCTTT CCAATGTGGC CCCTCGGTTC CCCAAGGAGT TATGTCTAGG TGGGCCTCTT
    GGCAACCACC ACGGAGGAGG ACCTAGCAAC AACAAGCTGC TGTCAGCAAG GGATGGGTTA
    GGAATGGGTC AAGGACAGAG ACCGTTTAAT GGCTCGAGCA ATGGACATGT TTACGAAAAG
    CTTTCCAGTA TAGAATCTGA TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53708
    Clone ID Related Accession (Same CDS sequence) XM_017355013.1 , XM_017355013.2
    Accession Version XM_017355013.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGAT
    AAGGTGAACC AGAACCCATT CATCCCAACC TTCGGCGACG ACCAGTGTCT ATTACACGGT
    GCCAAATCTT ACTACATCAA AAAGCAGCAG GCACAGCCGC AGCAGCAACA GACGCCGCAG
    CAGCAGCAGC AACAGCTCCT CAACAACAGT CGGGCTGACT TCCGGGGCTC CATGGGGGTG
    ACTTCCTATT TGCCAGCCTC TGCAACTTCT GGGGTGCTTT CCAATGTGGC CCCTCGGTTC
    CCCAAGGAGT TATGTCTAGG TGGGCCTCTT GGCAACCACC ACGGAGGAGG ACCTAGCAAC
    AACAAGCTGC TGTCAGCAAG GGATGGGTTA GGAATGGGTC AAGGACAGAG ACCGTTTAAT
    GGCTCGAGCA ATGGACATGT TTACGAAAAG CTTTCCAGTA TAGAATCTGA TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210502.1
    CDS1425..5081
    Translation

    Target ORF information:

    RefSeq Version XM_017355013.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGAT
    AAGGTGAACC AGAACCCATT CATCCCAACC TTCGGCGACG ACCAGTGTCT ATTACACGGT
    GCCAAATCTT ACTACATCAA AAAGCAGCAG GCACAGCCGC AGCAGCAACA GACGCCGCAG
    CAGCAGCAGC AACAGCTCCT CAACAACAGT CGGGCTGACT TCCGGGGCTC CATGGGGGTG
    ACTTCCTATT TGCCAGCCTC TGCAACTTCT GGGGTGCTTT CCAATGTGGC CCCTCGGTTC
    CCCAAGGAGT TATGTCTAGG TGGGCCTCTT GGCAACCACC ACGGAGGAGG ACCTAGCAAC
    AACAAGCTGC TGTCAGCAAG GGATGGGTTA GGAATGGGTC AAGGACAGAG ACCGTTTAAT
    GGCTCGAGCA ATGGACATGT TTACGAAAAG CTTTCCAGTA TAGAATCTGA TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53708
    Clone ID Related Accession (Same CDS sequence) XM_017355013.1 , XM_017355013.2
    Accession Version XM_017355013.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGAT
    AAGGTGAACC AGAACCCATT CATCCCAACC TTCGGCGACG ACCAGTGTCT ATTACACGGT
    GCCAAATCTT ACTACATCAA AAAGCAGCAG GCACAGCCGC AGCAGCAACA GACGCCGCAG
    CAGCAGCAGC AACAGCTCCT CAACAACAGT CGGGCTGACT TCCGGGGCTC CATGGGGGTG
    ACTTCCTATT TGCCAGCCTC TGCAACTTCT GGGGTGCTTT CCAATGTGGC CCCTCGGTTC
    CCCAAGGAGT TATGTCTAGG TGGGCCTCTT GGCAACCACC ACGGAGGAGG ACCTAGCAAC
    AACAAGCTGC TGTCAGCAAG GGATGGGTTA GGAATGGGTC AAGGACAGAG ACCGTTTAAT
    GGCTCGAGCA ATGGACATGT TTACGAAAAG CTTTCCAGTA TAGAATCTGA TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210502.1
    CDS1392..5048
    Translation

    Target ORF information:

    RefSeq Version XM_017355013.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355013.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGAT
    AAGGTGAACC AGAACCCATT CATCCCAACC TTCGGCGACG ACCAGTGTCT ATTACACGGT
    GCCAAATCTT ACTACATCAA AAAGCAGCAG GCACAGCCGC AGCAGCAACA GACGCCGCAG
    CAGCAGCAGC AACAGCTCCT CAACAACAGT CGGGCTGACT TCCGGGGCTC CATGGGGGTG
    ACTTCCTATT TGCCAGCCTC TGCAACTTCT GGGGTGCTTT CCAATGTGGC CCCTCGGTTC
    CCCAAGGAGT TATGTCTAGG TGGGCCTCTT GGCAACCACC ACGGAGGAGG ACCTAGCAAC
    AACAAGCTGC TGTCAGCAAG GGATGGGTTA GGAATGGGTC AAGGACAGAG ACCGTTTAAT
    GGCTCGAGCA ATGGACATGT TTACGAAAAG CTTTCCAGTA TAGAATCTGA TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa34765
    Clone ID Related Accession (Same CDS sequence) NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
    Accession Version NM_001128337.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-05
    Organism Danio rerio(zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX537268.5 and BX901927.7. On Nov 20, 2014 this sequence version replaced NM_001128337.1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript and protein alignments, and RNA-Seq data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GFIL01023244.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121809.1
    CDS125..5437
    Translation

    Target ORF information:

    RefSeq Version NM_001128337.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128337.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa34765
    Clone ID Related Accession (Same CDS sequence) NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
    Accession Version XM_017355011.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210500.1
    CDS1061..6373
    Translation

    Target ORF information:

    RefSeq Version XM_017355011.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355011.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa34765
    Clone ID Related Accession (Same CDS sequence) NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
    Accession Version XM_017355008.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210497.1
    CDS1394..6706
    Translation

    Target ORF information:

    RefSeq Version XM_017355008.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa34765
    Clone ID Related Accession (Same CDS sequence) NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
    Accession Version XM_017355008.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210497.1
    CDS1425..6737
    Translation

    Target ORF information:

    RefSeq Version XM_017355008.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa34765
    Clone ID Related Accession (Same CDS sequence) NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
    Accession Version XM_017355011.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product glutamate [NMDA] receptor subunit epsilon-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210500.1
    CDS792..6104
    Translation

    Target ORF information:

    RefSeq Version XM_017355011.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
    AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT
    GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC
    CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC
    CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC
    ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC
    GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG
    AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT
    GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG
    GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC
    CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT
    GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG
    TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA
    CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC
    ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT
    GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT
    GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA
    GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC
    TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA
    ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC
    AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG
    TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT
    ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT
    CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC
    GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC
    GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA
    CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT
    ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG
    ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG
    GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG
    GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC
    AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG
    ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT
    CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC
    ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG
    AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT
    TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC
    CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC
    CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG
    ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG
    AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA
    CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG
    TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG
    CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC
    CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG
    TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC
    TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC
    TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA
    TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC
    ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA
    CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC
    CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC
    ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC
    AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT
    ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT
    GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG
    CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG
    TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA
    TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG
    GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC
    TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG
    AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA
    CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC
    TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG
    TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC
    GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG
    GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT
    TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA
    ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG
    GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA
    AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG
    GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA
    GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG
    GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG
    GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT
    TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG
    ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC
    CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG
    TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA
    TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA
    GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC
    GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC
    CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG
    CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC
    AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA
    ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG
    CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT
    GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC
    GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.