GRIN2BB gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_017355013.1	XP_017210502.1	glutamate [NMDA] receptor subunit epsilon-2 isoform X3
XM_017355012.1	XP_017210501.1	glutamate [NMDA] receptor subunit epsilon-2 isoform X2
NM_001128337.2	NP_001121809.1	glutamate [NMDA] receptor subunit epsilon-2
XM_017355011.2	XP_017210500.1	glutamate [NMDA] receptor subunit epsilon-2 isoform X1
XM_017355013.2	XP_017210502.1	glutamate [NMDA] receptor subunit epsilon-2 isoform X3
XM_017355008.2	XP_017210497.1	glutamate [NMDA] receptor subunit epsilon-2 isoform X1
XM_017355012.2	XP_017210501.1	glutamate [NMDA] receptor subunit epsilon-2 isoform X2
XM_017355008.1	XP_017210497.1	glutamate [NMDA] receptor subunit epsilon-2 isoform X1
XM_017355011.1	XP_017210500.1	glutamate [NMDA] receptor subunit epsilon-2 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53707	XM_017355012.2 Latest version!	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa53708	XM_017355013.2 Latest version!	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa34765	NM_001128337.2 Latest version!	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa34765	XM_017355011.2 Latest version!	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa34765	XM_017355008.2 Latest version!	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa53708	XM_017355013.1	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa53707	XM_017355012.1	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa34765	XM_017355008.1	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa34765	XM_017355011.1	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

GRIN2BB ( XM_017355012.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210501.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa53707
Clone ID Related Accession (Same CDS sequence)	XM_017355012.1 , XM_017355012.2
Accession Version	XM_017355012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGAT AAGGTGAACC AGAACCCATT CATCCCAACC 
TTCGGCGACG ACCAGTGTCT ATTACACGGT GCCAAATCTT ACTACATCAA AAAGCAGCAG 
GCACAGCCGC AGCAGCAACA GACGCCGCAG CAGCAGCAGC AACAGCTCCT CAACAACAGT 
CGGGCTGACT TCCGGGGCTC CATGGGGGTG ACTTCCTATT TGCCAGCCTC TGCAACTTCT 
GGGGTGCTTT CCAATGTGGC CCCTCGGTTC CCCAAGGAGT TATGTCTAGG TGGGCCTCTT 
GGCAACCACC ACGGAGGAGG ACCTAGCAAC AACAAGCTGC TGTCAGCAAG GGATGGGTTA 
GGAATGGGTC AAGGACAGAG ACCGTTTAAT GGCTCGAGCA ATGGACATGT TTACGAAAAG 
CTTTCCAGTA TAGAATCTGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210501.1
CDS	1425..5891
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALGMSGPLPNRPRSLGSASSLEGGYDCDSLGGGVAPIFTTQPRQSLTHRNREKFDLIAGHPTQSSFKSGLPDLYGKFSFKGGASSSGFIAGHDRYCGGGGVGSGGDDGNIRSDVSDISTHTVTYGNLEGHSKRRKQYRDSLKKRPASAKSRREQDEIELGFRRRPHHTIHHHHHHHPATQAHRSATPPVERKSQRGGNCTSYLFRDKENLRDFYVDQFRAKEGASPWDLDLSDAPGMGGGVGLGGGSCGGVVSSGGAGGACTSLVPMEDFLKDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_017355012.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355012.1

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGAT AAGGTGAACC AGAACCCATT CATCCCAACC 
TTCGGCGACG ACCAGTGTCT ATTACACGGT GCCAAATCTT ACTACATCAA AAAGCAGCAG 
GCACAGCCGC AGCAGCAACA GACGCCGCAG CAGCAGCAGC AACAGCTCCT CAACAACAGT 
CGGGCTGACT TCCGGGGCTC CATGGGGGTG ACTTCCTATT TGCCAGCCTC TGCAACTTCT 
GGGGTGCTTT CCAATGTGGC CCCTCGGTTC CCCAAGGAGT TATGTCTAGG TGGGCCTCTT 
GGCAACCACC ACGGAGGAGG ACCTAGCAAC AACAAGCTGC TGTCAGCAAG GGATGGGTTA 
GGAATGGGTC AAGGACAGAG ACCGTTTAAT GGCTCGAGCA ATGGACATGT TTACGAAAAG 
CTTTCCAGTA TAGAATCTGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2BB ( XM_017355012.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210501.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa53707
Clone ID Related Accession (Same CDS sequence)	XM_017355012.1 , XM_017355012.2
Accession Version	XM_017355012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGAT AAGGTGAACC AGAACCCATT CATCCCAACC 
TTCGGCGACG ACCAGTGTCT ATTACACGGT GCCAAATCTT ACTACATCAA AAAGCAGCAG 
GCACAGCCGC AGCAGCAACA GACGCCGCAG CAGCAGCAGC AACAGCTCCT CAACAACAGT 
CGGGCTGACT TCCGGGGCTC CATGGGGGTG ACTTCCTATT TGCCAGCCTC TGCAACTTCT 
GGGGTGCTTT CCAATGTGGC CCCTCGGTTC CCCAAGGAGT TATGTCTAGG TGGGCCTCTT 
GGCAACCACC ACGGAGGAGG ACCTAGCAAC AACAAGCTGC TGTCAGCAAG GGATGGGTTA 
GGAATGGGTC AAGGACAGAG ACCGTTTAAT GGCTCGAGCA ATGGACATGT TTACGAAAAG 
CTTTCCAGTA TAGAATCTGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210501.1
CDS	1393..5859
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALGMSGPLPNRPRSLGSASSLEGGYDCDSLGGGVAPIFTTQPRQSLTHRNREKFDLIAGHPTQSSFKSGLPDLYGKFSFKGGASSSGFIAGHDRYCGGGGVGSGGDDGNIRSDVSDISTHTVTYGNLEGHSKRRKQYRDSLKKRPASAKSRREQDEIELGFRRRPHHTIHHHHHHHPATQAHRSATPPVERKSQRGGNCTSYLFRDKENLRDFYVDQFRAKEGASPWDLDLSDAPGMGGGVGLGGGSCGGVVSSGGAGGACTSLVPMEDFLKDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_017355012.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355012.2

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGAT AAGGTGAACC AGAACCCATT CATCCCAACC 
TTCGGCGACG ACCAGTGTCT ATTACACGGT GCCAAATCTT ACTACATCAA AAAGCAGCAG 
GCACAGCCGC AGCAGCAACA GACGCCGCAG CAGCAGCAGC AACAGCTCCT CAACAACAGT 
CGGGCTGACT TCCGGGGCTC CATGGGGGTG ACTTCCTATT TGCCAGCCTC TGCAACTTCT 
GGGGTGCTTT CCAATGTGGC CCCTCGGTTC CCCAAGGAGT TATGTCTAGG TGGGCCTCTT 
GGCAACCACC ACGGAGGAGG ACCTAGCAAC AACAAGCTGC TGTCAGCAAG GGATGGGTTA 
GGAATGGGTC AAGGACAGAG ACCGTTTAAT GGCTCGAGCA ATGGACATGT TTACGAAAAG 
CTTTCCAGTA TAGAATCTGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2BB ( XM_017355013.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210502.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA.

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CloneID	ODa53708
Clone ID Related Accession (Same CDS sequence)	XM_017355013.1 , XM_017355013.2
Accession Version	XM_017355013.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGAT 
AAGGTGAACC AGAACCCATT CATCCCAACC TTCGGCGACG ACCAGTGTCT ATTACACGGT 
GCCAAATCTT ACTACATCAA AAAGCAGCAG GCACAGCCGC AGCAGCAACA GACGCCGCAG 
CAGCAGCAGC AACAGCTCCT CAACAACAGT CGGGCTGACT TCCGGGGCTC CATGGGGGTG 
ACTTCCTATT TGCCAGCCTC TGCAACTTCT GGGGTGCTTT CCAATGTGGC CCCTCGGTTC 
CCCAAGGAGT TATGTCTAGG TGGGCCTCTT GGCAACCACC ACGGAGGAGG ACCTAGCAAC 
AACAAGCTGC TGTCAGCAAG GGATGGGTTA GGAATGGGTC AAGGACAGAG ACCGTTTAAT 
GGCTCGAGCA ATGGACATGT TTACGAAAAG CTTTCCAGTA TAGAATCTGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210502.1
CDS	1425..5081
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_017355013.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355013.1

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGAT 
AAGGTGAACC AGAACCCATT CATCCCAACC TTCGGCGACG ACCAGTGTCT ATTACACGGT 
GCCAAATCTT ACTACATCAA AAAGCAGCAG GCACAGCCGC AGCAGCAACA GACGCCGCAG 
CAGCAGCAGC AACAGCTCCT CAACAACAGT CGGGCTGACT TCCGGGGCTC CATGGGGGTG 
ACTTCCTATT TGCCAGCCTC TGCAACTTCT GGGGTGCTTT CCAATGTGGC CCCTCGGTTC 
CCCAAGGAGT TATGTCTAGG TGGGCCTCTT GGCAACCACC ACGGAGGAGG ACCTAGCAAC 
AACAAGCTGC TGTCAGCAAG GGATGGGTTA GGAATGGGTC AAGGACAGAG ACCGTTTAAT 
GGCTCGAGCA ATGGACATGT TTACGAAAAG CTTTCCAGTA TAGAATCTGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2BB ( XM_017355013.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210502.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA.

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CloneID	ODa53708
Clone ID Related Accession (Same CDS sequence)	XM_017355013.1 , XM_017355013.2
Accession Version	XM_017355013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGAT 
AAGGTGAACC AGAACCCATT CATCCCAACC TTCGGCGACG ACCAGTGTCT ATTACACGGT 
GCCAAATCTT ACTACATCAA AAAGCAGCAG GCACAGCCGC AGCAGCAACA GACGCCGCAG 
CAGCAGCAGC AACAGCTCCT CAACAACAGT CGGGCTGACT TCCGGGGCTC CATGGGGGTG 
ACTTCCTATT TGCCAGCCTC TGCAACTTCT GGGGTGCTTT CCAATGTGGC CCCTCGGTTC 
CCCAAGGAGT TATGTCTAGG TGGGCCTCTT GGCAACCACC ACGGAGGAGG ACCTAGCAAC 
AACAAGCTGC TGTCAGCAAG GGATGGGTTA GGAATGGGTC AAGGACAGAG ACCGTTTAAT 
GGCTCGAGCA ATGGACATGT TTACGAAAAG CTTTCCAGTA TAGAATCTGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210502.1
CDS	1392..5048
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_017355013.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355013.2

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGAT 
AAGGTGAACC AGAACCCATT CATCCCAACC TTCGGCGACG ACCAGTGTCT ATTACACGGT 
GCCAAATCTT ACTACATCAA AAAGCAGCAG GCACAGCCGC AGCAGCAACA GACGCCGCAG 
CAGCAGCAGC AACAGCTCCT CAACAACAGT CGGGCTGACT TCCGGGGCTC CATGGGGGTG 
ACTTCCTATT TGCCAGCCTC TGCAACTTCT GGGGTGCTTT CCAATGTGGC CCCTCGGTTC 
CCCAAGGAGT TATGTCTAGG TGGGCCTCTT GGCAACCACC ACGGAGGAGG ACCTAGCAAC 
AACAAGCTGC TGTCAGCAAG GGATGGGTTA GGAATGGGTC AAGGACAGAG ACCGTTTAAT 
GGCTCGAGCA ATGGACATGT TTACGAAAAG CTTTCCAGTA TAGAATCTGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2BB ( NM_001128337.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001121809.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), mRNA.

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CloneID	ODa34765
Clone ID Related Accession (Same CDS sequence)	NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
Accession Version	NM_001128337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX537268.5 and BX901927.7. On Nov 20, 2014 this sequence version replaced NM_001128337.1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript and protein alignments, and RNA-Seq data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GFIL01023244.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121809.1
CDS	125..5437
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALGMSGPLPNRPRSLGSASSLEGGYDCDSLGGGVAPIFTTQPRQSLTHRNREKFDLIAGHPTQSSFKSGLPDLYGKFSFKGGASSSGFIAGHDRYCGGGGVGSGGDDGNIRSDVSDISTHTVTYGNLEGHSKRRKQYRDSLKKRPASAKSRREQDEIELGFRRRPHHTIHHHHHHHPATQAHRSATPPVERKSQRGGNCTSYLFRDKENLRDFYVDQFRAKEGASPWDLDLSDAPGMGGGVGLGGGSCGGVVSSGGAGGACTSLVPMEDFLKGKSKKTECKGGMGGGSPGQQGHACWEKGIGGVGGLAGGDWECRSCHSGGVGSGGSKPVCMHGGGGAGGYPAAGGVGGSSGGSGQISSRPSSATCKRCDSCKKPGNLYDISEDNLLLDQIAGKHPLESGKGGGGTGAQTQVQRRKFGPGGKVLRRQHSYDTFVELQKEGAGRMGGFGGGGGASMLPPPRSVSLKDKDRYMEGASPYAQMFEQYAGGERETSYFGDRGKGGGSSFSLFRGGEGGLHRRSVGERDMRDRDRGMMGGGVGGTRGVGTYSLSKSLYPDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	NM_001128337.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128337.2

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2BB ( XM_017355011.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210500.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA.

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CloneID	ODa34765
Clone ID Related Accession (Same CDS sequence)	NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
Accession Version	XM_017355011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210500.1
CDS	1061..6373
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALGMSGPLPNRPRSLGSASSLEGGYDCDSLGGGVAPIFTTQPRQSLTHRNREKFDLIAGHPTQSSFKSGLPDLYGKFSFKGGASSSGFIAGHDRYCGGGGVGSGGDDGNIRSDVSDISTHTVTYGNLEGHSKRRKQYRDSLKKRPASAKSRREQDEIELGFRRRPHHTIHHHHHHHPATQAHRSATPPVERKSQRGGNCTSYLFRDKENLRDFYVDQFRAKEGASPWDLDLSDAPGMGGGVGLGGGSCGGVVSSGGAGGACTSLVPMEDFLKGKSKKTECKGGMGGGSPGQQGHACWEKGIGGVGGLAGGDWECRSCHSGGVGSGGSKPVCMHGGGGAGGYPAAGGVGGSSGGSGQISSRPSSATCKRCDSCKKPGNLYDISEDNLLLDQIAGKHPLESGKGGGGTGAQTQVQRRKFGPGGKVLRRQHSYDTFVELQKEGAGRMGGFGGGGGASMLPPPRSVSLKDKDRYMEGASPYAQMFEQYAGGERETSYFGDRGKGGGSSFSLFRGGEGGLHRRSVGERDMRDRDRGMMGGGVGGTRGVGTYSLSKSLYPDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_017355011.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355011.2

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2BB ( XM_017355008.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210497.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA.

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CloneID	ODa34765
Clone ID Related Accession (Same CDS sequence)	NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
Accession Version	XM_017355008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210497.1
CDS	1394..6706
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALGMSGPLPNRPRSLGSASSLEGGYDCDSLGGGVAPIFTTQPRQSLTHRNREKFDLIAGHPTQSSFKSGLPDLYGKFSFKGGASSSGFIAGHDRYCGGGGVGSGGDDGNIRSDVSDISTHTVTYGNLEGHSKRRKQYRDSLKKRPASAKSRREQDEIELGFRRRPHHTIHHHHHHHPATQAHRSATPPVERKSQRGGNCTSYLFRDKENLRDFYVDQFRAKEGASPWDLDLSDAPGMGGGVGLGGGSCGGVVSSGGAGGACTSLVPMEDFLKGKSKKTECKGGMGGGSPGQQGHACWEKGIGGVGGLAGGDWECRSCHSGGVGSGGSKPVCMHGGGGAGGYPAAGGVGGSSGGSGQISSRPSSATCKRCDSCKKPGNLYDISEDNLLLDQIAGKHPLESGKGGGGTGAQTQVQRRKFGPGGKVLRRQHSYDTFVELQKEGAGRMGGFGGGGGASMLPPPRSVSLKDKDRYMEGASPYAQMFEQYAGGERETSYFGDRGKGGGSSFSLFRGGEGGLHRRSVGERDMRDRDRGMMGGGVGGTRGVGTYSLSKSLYPDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_017355008.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355008.2

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2BB ( XM_017355008.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210497.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA.

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CloneID	ODa34765
Clone ID Related Accession (Same CDS sequence)	NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
Accession Version	XM_017355008.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210497.1
CDS	1425..6737
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALGMSGPLPNRPRSLGSASSLEGGYDCDSLGGGVAPIFTTQPRQSLTHRNREKFDLIAGHPTQSSFKSGLPDLYGKFSFKGGASSSGFIAGHDRYCGGGGVGSGGDDGNIRSDVSDISTHTVTYGNLEGHSKRRKQYRDSLKKRPASAKSRREQDEIELGFRRRPHHTIHHHHHHHPATQAHRSATPPVERKSQRGGNCTSYLFRDKENLRDFYVDQFRAKEGASPWDLDLSDAPGMGGGVGLGGGSCGGVVSSGGAGGACTSLVPMEDFLKGKSKKTECKGGMGGGSPGQQGHACWEKGIGGVGGLAGGDWECRSCHSGGVGSGGSKPVCMHGGGGAGGYPAAGGVGGSSGGSGQISSRPSSATCKRCDSCKKPGNLYDISEDNLLLDQIAGKHPLESGKGGGGTGAQTQVQRRKFGPGGKVLRRQHSYDTFVELQKEGAGRMGGFGGGGGASMLPPPRSVSLKDKDRYMEGASPYAQMFEQYAGGERETSYFGDRGKGGGSSFSLFRGGEGGLHRRSVGERDMRDRDRGMMGGGVGGTRGVGTYSLSKSLYPDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_017355008.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355008.1

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2BB ( XM_017355011.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210500.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA.

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CloneID	ODa34765
Clone ID Related Accession (Same CDS sequence)	NM_001128337.2 , XM_017355011.2 , XM_017355008.2 , XM_017355008.1 , XM_017355011.1
Accession Version	XM_017355011.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glutamate [NMDA] receptor subunit epsilon-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210500.1
CDS	792..6104
Translation	MGVGLAMFKGLYLHSSAMLVSLHLSSSPFSDCRVLSFVSLVSSFKLPLYISLLLLSLFFFPTCESRRGGGIGTPTGGMNSQSIISPPHYPPPGVSPVGPPMSPKFAQGLSIAVILVGNSSEVSLSEGLEKEDFLHVPLPPKVELVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGHQDFVNRIRSTVDNSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPSLVAGDTDNVPNVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLLKSGCHGAPDKKGSKSGNPNEVLRYLMNVTFEGRNLSFSEDGFQMHPKLVIILLNKERQYERVGKWENGSLAMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKLQNLTGDSGIYIKRCCKGFCIDILKKIAKSVKFTYDLYLVTNGKHGKKINGTWNGMVGEVVLKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVTGLSDKKFQHPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVNEALHSLKSGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRAVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGMTFSISRGIYSCIHGVQIEENKSALNSPSATMKMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYSHRESAVYDISEHRRSLAGHSDCKPPPYLPEDNMFSDYVSEVERTFGNLHLKDSNLYQDHYLHHHGGSELALGMSGPLPNRPRSLGSASSLEGGYDCDSLGGGVAPIFTTQPRQSLTHRNREKFDLIAGHPTQSSFKSGLPDLYGKFSFKGGASSSGFIAGHDRYCGGGGVGSGGDDGNIRSDVSDISTHTVTYGNLEGHSKRRKQYRDSLKKRPASAKSRREQDEIELGFRRRPHHTIHHHHHHHPATQAHRSATPPVERKSQRGGNCTSYLFRDKENLRDFYVDQFRAKEGASPWDLDLSDAPGMGGGVGLGGGSCGGVVSSGGAGGACTSLVPMEDFLKGKSKKTECKGGMGGGSPGQQGHACWEKGIGGVGGLAGGDWECRSCHSGGVGSGGSKPVCMHGGGGAGGYPAAGGVGGSSGGSGQISSRPSSATCKRCDSCKKPGNLYDISEDNLLLDQIAGKHPLESGKGGGGTGAQTQVQRRKFGPGGKVLRRQHSYDTFVELQKEGAGRMGGFGGGGGASMLPPPRSVSLKDKDRYMEGASPYAQMFEQYAGGERETSYFGDRGKGGGSSFSLFRGGEGGLHRRSVGERDMRDRDRGMMGGGVGGTRGVGTYSLSKSLYPDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQAQPQQQQTPQQQQQQLLNNSRADFRGSMGVTSYLPASATSGVLSNVAPRFPKELCLGGPLGNHHGGGPSNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_017355011.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b (grin2bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355011.1

ORF Insert Sequence:

ATGGGAGTTG GGCTGGCAAT GTTCAAGGGA CTGTACCTTC ACTCCTCCGC CATGCTTGTC 
AGCCTCCACC TCTCCTCTTC CCCTTTCTCC GACTGCCGCG TCCTGTCTTT CGTCTCCCTT 
GTTTCCTCCT TCAAACTGCC CCTCTACATC TCGCTCCTTC TCCTATCGCT CTTCTTCTTC 
CCCACCTGCG AGTCTCGCCG CGGAGGTGGG ATCGGGACGC CCACCGGAGG CATGAACAGC 
CAATCGATTA TCAGTCCTCC TCATTATCCA CCACCAGGAG TCAGTCCAGT GGGTCCTCCC 
ATGTCACCAA AGTTTGCTCA AGGCCTTAGC ATCGCGGTGA TCCTAGTGGG AAACTCCAGC 
GAGGTGTCGC TTTCTGAAGG TCTGGAGAAA GAGGACTTTC TACACGTGCC TCTTCCACCG 
AAAGTCGAGC TGGTCACCAT GAATGAAACC GACCCTAAGA GTATCATCAA CCGCATCTGT 
GCTCTGATGT CACGCAACTG GCTCCAGGGT GTGGTGTTCG GGGATGATAC CGATCAGGAG 
GCCATCGCCC AGATATTGGA CTTCATCTCG GCTCAGACGC ACATCCCCAT TCTGGGTATC 
CGTGGAGGGT CTTCCATGAT CATGGCTGCC AAGGATGACC ATTCCATGTT CTTCCAGTTT 
GGTCCCTCTA TCGAGCAGCA GGCCTCTGTT ATGCTGAACA TAATGGAGGA GTATGATTGG 
TACATTTTCT CCATCGTCAC CACCTATTAC CCTGGACACC AGGACTTTGT CAATCGGATA 
CGGAGTACCG TTGATAACAG TTTTGTCGGC TGGGAGCTGG AAGAGGTGCT GCTGTTGGAC 
ATGTCAGTGG ATGATGGAGA CTCGAAGATC CAGAACCAAA TGAAGAAGCT CCAGAGTCCT 
GTTATACTGC TGTACTGCAC TAAAGAAGAA GCCACCACCA TTTTTGAAGT GGCACATTCT 
GTTGGCCTCA CTGGTTACGG TTATACCTGG ATCGTACCAT CTTTGGTGGC AGGGGATACA 
GATAATGTGC CCAATGTCTT TCCAACAGGG TTGATCTCTG TCTCTTATGA TGAGTGGGAC 
TATGGCCTGG AGGCTCGAGT GCGTGATGCT GTAGCCATAA TCGCCATGGC AACCTCCACA 
ATGATGCTGG ACCGGGGTCC TCACACGCTG CTGAAGTCTG GATGCCACGG CGCACCTGAC 
AAAAAAGGCA GCAAGTCAGG AAACCCCAAC GAGGTGCTGA GGTATCTCAT GAATGTGACG 
TTTGAAGGAC GCAACCTCTC CTTCAGCGAG GACGGTTTTC AGATGCACCC AAAGTTGGTT 
ATTATACTGT TAAATAAGGA GAGGCAGTAC GAAAGGGTCG GTAAATGGGA GAACGGGTCT 
CTGGCGATGA AGTACCACGT GTGGCCCCGC TTCGAGCTGT ACTCGGACGC TGAGGAGCGC 
GAGGACGACC ACCTGTCCAT CGTCACACTG GAGGAGGCAC CGTTCGTGAT TGTGGAGGAC 
GTTGATCCTC TCAGCGGCAC CTGCATGAGG AATACAGTCC CCTGCCGAAA ACAGCTCAAA 
CTCCAAAATC TGACAGGAGA TTCAGGGATC TACATTAAAC GCTGTTGTAA GGGCTTCTGT 
ATAGACATCC TCAAGAAGAT CGCCAAGTCT GTCAAATTCA CCTACGATCT CTACTTGGTG 
ACCAATGGGA AACACGGCAA GAAGATCAAC GGCACATGGA ACGGAATGGT TGGAGAGGTG 
GTGCTGAAGA ATGCCCACAT GGCAGTGGGG TCTCTGACCA TAAACGAAGA GAGGTCAGAG 
GTCATTGACT TCTCAGTGCC CTTCATCGAG ACCGGGATCA GTGTCATGGT ATCTCGTAGC 
AATGGCACAG TATCTCCATC TGCCTTTTTA GAGCCCTTCA GCGCTGATGT GTGGGTAATG 
ATGTTCGTTA TGCTGTTGAT AGTTTCAGCC GTGGCCGTCT TCGTCTTTGA GTACTTCAGT 
CCTGTCGGCT ATAATCGCTG CTTGGCAGAC GGACGAGAGC CTGGCGGTCC CTCCTTCACC 
ATCGGAAAAG CTATTTGGCT TCTTTGGGGT TTGGTCTTCA ATAACTCTGT CCCCGTGCAG 
AACCCCAAAG GCACTACTAG CAAAATCATG GTTTCGGTCT GGGCCTTCTT CGCTGTGATT 
TTCCTGGCCA GCTACACTGC CAACCTGGCT GCATTCATGA TTCAAGAGGA ATATGTGGAC 
CAGGTGACTG GCCTGAGCGA TAAAAAGTTT CAACATCCGA ATGACTTCTC CCCTCCGTTC 
CGCTTCGGTA CTGTGCCCAA TGGCAGCACC GAGAGAAACA TAAGGAACAA CTACAAGGAG 
ATGCACAGTT ATATGACCAG CTTCCATCAG AAGAATGTAA ACGAGGCCCT TCACAGCCTG 
AAGTCGGGTA AATTGGATGC ATTCATCTAC GATGCAGCGG TCCTGAACTA CATGGCCGGA 
CGTGATGAGG GCTGTAAGCT GGTGACCATT GGCAGTGGTT ATATCTTCGC TACTACAGGG 
TATGGCATTG CCATTCAGAA AGACTCAGGC TGGAAAAGAG CAGTGGACCT CGCTATTCTG 
CAGCTCTTTG GAGATGGTGA GATGGAGGAA CTCGAGGCCC TGTGGCTGAC TGGCATCTGC 
CACAATGAGA AAAATGAGGT GATGAGCAGT CAGCTGGATG TAGACAACAT GGCCGGTGTG 
TTCTACATGC TGGGAGCTGC AATGGCCCTG TCCCTTATTA CGTTCATAGC AGAGCACCTC 
TTCTACTGGC AGCTGAGATT CTGCTTCATG GGTGTGTGCT CGGGCAAGCC TGGCATGACC 
TTCTCCATCA GCAGGGGGAT CTACAGTTGC ATCCACGGTG TTCAGATTGA AGAGAATAAA 
TCAGCTCTTA ACTCACCATC AGCCACTATG AAGATGAACA TGAACAACAC CCACTCCAAC 
ATTCTCCGCT TGTTGCGTAC TGCCAAAAAC ATGACCTCTG TGCCTGGGGT CAATGGTTCA 
CCCCACAGCG CATTGGACTA CAGCCACCGT GAATCAGCCG TTTATGACAT CAGCGAACAC 
CGGCGCAGCC TAGCAGGCCA CTCAGACTGC AAGCCTCCAC CGTATCTACC AGAGGACAAC 
ATGTTCAGCG ACTATGTAAG CGAGGTGGAG AGGACTTTTG GGAACTTGCA CCTGAAGGAC 
AGCAACCTGT ACCAGGATCA TTACCTACAC CACCATGGGG GATCTGAACT GGCTCTCGGT 
ATGTCGGGTC CCCTGCCTAA CCGACCTCGT AGCTTAGGGA GTGCCAGCTC ATTGGAGGGT 
GGGTACGATT GTGACAGTTT AGGGGGAGGG GTAGCGCCCA TCTTTACCAC CCAGCCACGG 
CAGTCGCTGA CACATCGGAA CAGGGAAAAG TTTGACCTGA TTGCAGGTCA CCCAACCCAG 
TCAAGCTTCA AGTCTGGCCT GCCTGATCTA TATGGAAAGT TCTCCTTCAA GGGTGGGGCA 
TCCAGTTCAG GGTTTATTGC AGGACATGAC AGGTACTGCG GGGGAGGTGG GGTGGGATCG 
GGAGGGGACG ATGGAAACAT TCGATCCGAC GTGTCAGATA TTTCCACTCA CACTGTGACC 
TACGGGAACC TTGAAGGGCA CAGCAAACGG CGCAAGCAGT ACAGGGACAG CCTAAAAAAG 
AGACCCGCTT CTGCCAAGTC TCGCCGTGAA CAGGATGAAA TTGAGCTTGG CTTCAGGAGA 
CGTCCCCACC ACACTATCCA TCATCATCAC CACCATCATC CAGCAACACA AGCACACCGC 
TCAGCCACCC CTCCTGTGGA GCGCAAGAGC CAGAGAGGTG GTAACTGCAC CTCCTATCTG 
TTCAGAGACA AAGAGAATTT GAGGGACTTT TATGTAGACC AGTTTCGAGC CAAGGAAGGC 
GCATCCCCAT GGGATTTGGA TTTAAGTGAT GCTCCTGGAA TGGGTGGAGG AGTGGGACTG 
GGCGGAGGCA GCTGCGGTGG AGTAGTTAGT AGCGGTGGTG CTGGGGGAGC ATGCACTAGT 
TTGGTTCCCA TGGAAGATTT CCTGAAAGGT AAATCCAAGA AGACAGAATG TAAGGGTGGA 
ATGGGAGGAG GATCACCTGG GCAACAGGGC CATGCATGCT GGGAAAAGGG CATCGGAGGG 
GTGGGAGGGT TGGCCGGCGG GGATTGGGAA TGTCGCAGTT GCCACAGTGG AGGAGTTGGA 
AGTGGAGGAA GCAAGCCAGT TTGCATGCAT GGGGGAGGCG GGGCCGGTGG GTATCCAGCG 
GCTGGGGGAG TTGGGGGATC CAGTGGAGGG AGTGGACAAA TCTCAAGCCG CCCTAGTTCA 
GCCACCTGCA AGCGATGTGA TTCGTGCAAG AAACCGGGCA ACTTGTATGA CATTAGTGAG 
GACAACCTCC TCCTGGATCA AATTGCGGGC AAGCACCCTC TGGAGAGCGG AAAGGGAGGG 
GGAGGCACAG GTGCCCAAAC CCAGGTACAG AGGCGGAAAT TTGGCCCAGG TGGTAAGGTT 
TTGCGCAGGC AGCACTCTTA TGACACATTT GTAGAACTAC AGAAGGAAGG GGCGGGCAGG 
ATGGGTGGGT TTGGAGGCGG TGGTGGAGCA TCAATGCTTC CTCCACCCCG AAGCGTGAGC 
CTAAAAGACA AAGACCGCTA CATGGAGGGC GCGAGCCCCT ACGCCCAGAT GTTTGAGCAG 
TATGCGGGAG GGGAGCGGGA GACATCATAT TTTGGGGACA GAGGGAAGGG TGGGGGTTCA 
TCATTTAGCT TATTCCGTGG GGGTGAAGGT GGGTTGCACC GTCGCTCCGT GGGTGAGAGA 
GACATGAGGG ACAGGGATAG AGGCATGATG GGAGGAGGTG TGGGCGGTAC CCGAGGGGTC 
GGGACTTACT CCTTGTCTAA ATCTCTCTAC CCAGATAAGG TGAACCAGAA CCCATTCATC 
CCAACCTTCG GCGACGACCA GTGTCTATTA CACGGTGCCA AATCTTACTA CATCAAAAAG 
CAGCAGGCAC AGCCGCAGCA GCAACAGACG CCGCAGCAGC AGCAGCAACA GCTCCTCAAC 
AACAGTCGGG CTGACTTCCG GGGCTCCATG GGGGTGACTT CCTATTTGCC AGCCTCTGCA 
ACTTCTGGGG TGCTTTCCAA TGTGGCCCCT CGGTTCCCCA AGGAGTTATG TCTAGGTGGG 
CCTCTTGGCA ACCACCACGG AGGAGGACCT AGCAACAACA AGCTGCTGTC AGCAAGGGAT 
GGGTTAGGAA TGGGTCAAGG ACAGAGACCG TTTAATGGCT CGAGCAATGG ACATGTTTAC 
GAAAAGCTTT CCAGTATAGA ATCTGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GRIN2BB
Entrez Gene ID	559976
Full Name	glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b
Synonyms	si:ch211-243a20.1
General protein information	Preferred Names glutamate receptor, ionotropic, N-methyl D-aspartate 2B, genome duplicate b Names glutamate [NMDA] receptor subunit epsilon-2 NR2B.2 grin2b
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

	GRIN2B	XP_001088140.1
Bos taurus (cattle)	GRIN2B	NP_001179850.1
Danio rerio (zebrafish)	LOC100334883	XP_003198060.2
Xenopus tropicalis (tropical clawed frog)	grin2b	XP_002932208.2
Pan troglodytes (chimpanzee)	GRIN2B	XP_528744.2
Canis lupus familiaris (dog)	GRIN2B	NP_001008719.1
Mus musculus (house mouse)	Grin2b	NP_032197.3
Danio rerio (zebrafish)	LOC559976	NP_001121809.1
Homo sapiens (human)	GRIN2B	NP_000825.2
Rattus norvegicus (Norway rat)	Grin2b	NP_036706.1
Gallus gallus (chicken)	GRIN2B	XP_416204.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GRIN2BB cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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