arhgef28 cDNA ORF clone, Danio rerio(zebrafish)

The following arhgef28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa144580 XM_017356273.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa59223 XM_017356273.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa59223 XM_021476535.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa144575 XM_017356269.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144576 XM_009301598.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144577 XM_017356270.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144578 XM_017356271.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144579 XM_017356272.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa59219 XM_021476529.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa59219 XM_017356269.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa59220 XM_021476532.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa59220 XM_017356270.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa59221 XM_017356271.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa59221 XM_021476533.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa59222 XM_017356272.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa59222 XM_021476534.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa43200 XM_009301598.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa43200 XM_021476530.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa144580
    Clone ID Related Accession (Same CDS sequence) XM_017356273.2
    Accession Version XM_017356273.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394590.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCTG ACTCAGACTC TCCGTTTAAC TACTCGTGGC CATCCTTTCC AAAGATGAGA 
    ATCCGGAGAC GGACATCAAA AGCAGAGAAG CAGTTTTCGT CTCCGGGCTC CTGCACTCCG
    TTTCACCTTC ATTCCTCAGC CGCTGCAGCC GTCAGGCTCA CTGCCATGCT CAAAGGCTCC
    ACTAAGGACA GTGAGCTGAA TATGAAGTAC GCCGTTCCAG GTGCCACAGA GGACAGCTCT
    GGGACCGATA GTGGCATGTG GCGGATGTCA GATACGGGGG TCTCATCACC AGGGACAATA
    GATATATCTC CTGCTGTTAA AGATGATGAA GGGCAGAGAG GACGGCACCC CGCCTTTCGT
    TCTTTCTCTC CCACCTCTCC AGCAGGACTG ATGCTGGATA GATTCATGCG GGCCACGAGA
    ACCACTGAAC TGACCTACAA CAGCTCAAGC TCACCTACAG AGTCATGTGA CCTCAGAAGT
    CCAATCCTCT CTCTAAGCCC GAGTCTGGTT GCCTTGGAAA TGGACAGCGA GGAGGATGAC
    GATGATGACA TTTTAATGAA GAAGCCCCTC CCACAACTGC AACCGGATTC CAAAGACAGC
    ACAGATGAGC AGGTTATGTC CAGCCCCGCA CCTGTACTCA TCTGCACGCG CTCTCACTCT
    GCCTCCTCTG CCTGTAACCC CGCCCCTGCA AAGGATGTTG AGCAGGGTGT CCGACTCCGA
    TCCTATTCCT ACACCTCCCC CAAAATCAGC CTTCTGCCTC CACGTTTCAG CCGAGACGCC
    CATCCTCCAC CCGCCGACCT CAGCCAGGAT GGCGTTCTCT CCAGCAGCAG TGGCAGCAAG
    TCTCTTCTAC ACGCCCTGTC TCTGTCTAAA TCTCTGTCTC TGCTCAACCC TGTTAAGCAC
    CGCTCTTCCA GCACATCAGA GCAGCCTCAA GAGAAAAGGG AGTTAAGGTT TCGCAGGCGA
    GCTCAGTCGG CTGATGATGA AAGCAGTGCA GAGCTGGCCG ACTCGCTCCA ACATCTCACG
    CTCTCCGAGT TCCTCAAAGA GATTGAGGAA GAAGAATGGG ATAAATATAT CATCCCTTCC
    AAAACAGAGT CAGAGAAGTA CAAAGTCAGC CGCACATTCA GCTTCATAAA GAGCCGAATG
    TATAGCACTC GCAACAAGAA CAAGGGGAAA GGAAAAGAGA AAGAGACCAA AGACAAGCAG
    GTCAACGGAC ACCAGTTCAC AGCGGGATCT GTCCTGGGGT CGACACTCTG TGAGATCTGC
    AGCAAGCCAG CTTCAGGGAA AGAGGTTGTC CATTGCACAC ACTGTGCAGT GAGTGTCCAT
    AAGGGCTGTA AGGACTCATC TACGCCATGC CTGAAGAAAC CTCAAGACAA ATATTCAGTG
    ATGACAGTAA AAAGCAAGAC AGCCTCTCTT CCTCAAAATT TTATTGTGCG TGAGAGTTCA
    TCGTCGTCCC TTCAGATCTC TACCTCTGCT TCTCTCCCTG TCATGACATC ACGAGACTCG
    GCTCCTCTTC CTTGTCCTCT GTCCAGAAGT GTGCCCTTAA CCACTGAAAG GTTTAGCGAG
    AGCCCAGATG CTGATAGTGA TTCTCCTCTT TGGAGGAACC ACATCCAATC AGAGGAGCTT
    CTCCAAACCA TGGAGTCCTC AACATCCACA GACTCGTCCA TCATTGAAGA CTCGGTTGAT
    TCCCCTTTAC AGGTTGAGCT GCTGATGAAT GCTGTAGATT TGGAGGCAGA GTCATGGAGT
    TTAGCTGTGG AGGCCCAGTT CTGTAGGATG CAAGAAAAAC GCATCATCAA GAGACAGGAT
    GTGATTTACG AGTTCATGCA GACCGAGCTC CATCACGTGC AGACACTCAT GGTGATGGCA
    GAGGTCTTCA GAAGGGGAAT GCGGGATGAA GTGGGTCTGG ACGCAGAGAC CATTGGCCGG
    ATTTTTCCTT GCTTAGATGA GCTGCTCCAA CTCCATCGTG ATTTTCTGAC AGCAATGAGG
    GAACGAAGGC AAAGCTGCGT CCAGAACAGC AGCAGCAAAA ACTTTCTCAT CCACCGTGTG
    GGTGATATTT TTCTGCAACA GTTCTCCCAG GAGAACGCAG AGAAGATGAA GCAGGTGTAT
    GGAGAGTTTT GCAGTCATCA CACAGAAGCA GTCAGTTACT TCAAAGAGCT TCAGCAGCAG
    AACAAGAGAT TTCAGCTCTT CATCAAACAA CAGAGTAGTA ACTCACTGGT GAAGAGGCGA
    GAGATCCCAG AATGTATTTT GTTAGTGACG CAGCGAATTA CAAAGTACCC TGTCCTCCTA
    GAGAGAATAC TCCAGTACAC AGACGAGGGC ACAGAAGAGC ACTCTGACCT CTCTCGAGCT
    CTAGTCCTGA TCCGAGAGCT GATCTCCGCT GTGGACCAAT ATGTGAGTCA GTATGAGCAG
    AACCAGAAGC TGAGTGAAGT CTTGGGCCGC ATGGAGAACA AGTGCTCTAC CAAACTGAAG
    ACCGGCTGCA CTTTCCGCAA GCAGGACATC ACCAGCGGCA GGAATCTGCT GCACCAGGGC
    CCGCTGCTCT GGAAGACAGC CACTGGAAGA CTGAAAGATG TTCTGGCATT TTTGCTGTCT
    GATTCACTGG TGTTCCTTCA GGAGAAAGAT CAGAAATACA TTTTTGCTAC TGTGGACCAG
    AAGCCTCCAG TGATCTCTCT GCAGAAACTA ATTGTGCGTG AGGTGGCCAA TGAAGAGAGA
    GGCATGTTTC TGATCAGTGC ATCTTCAGCC GGGCCTGAGA TGTACGAGGT GCACGCAGCC
    TCTAAAGATG AACGCAACGC CTGGATGAGA CTCATCAGAG AGGCCGTGGA GAATTGCCCT
    GAAGAGGAGG ATGAAACTAC AAGTGAGTCA GAAGAGGAAA GGAGAGTCAC AGAAGCAAAG
    GTCCAAAAGA TCCACCGGCT GCAAGAGACG CTGTGTGGCC ATGATCACCT CATCTGTTCC
    AATCTGGAGG AGAAGCTGCA CATCTATGCC GAGCTGTCAG TGATGGCAGG CCAGCGAGAG
    GCAGGACCAG AGCCCAGGCT GCTGGTACGA CCCAGCACTG AAGACGTTCC CCAGGCTGGA
    GTACTACTCA GCGCCGCTCT CAGAGAGGCG GAGAGCCTAA GAGCAGCTCT CTCCTCTCAT
    TGTGCTTCCA CTCCTCCCGT CCCGTCCGGT CCAGAAGATC TAAACAAACC TGAACCCTGC
    ACTCCTCCAT CTCCTCCTCT GCCTCCTGAA AGTCCACTGA CAGACCCCAT CCAGAGTGAG
    GAGGAGGAGT GGAGAACTGA GGAAAGCTCA GAAGCACAGA CCTTTAATCA CAACTCGGAC
    TCTGACCACA ATCTACAGCT GAAGGTGAGT CAGAGTGTGC AGAGCTTGAC TCAGCTGCTG
    TACAGTCTAC AGGCTGCAGT TACCATCCAA GACAGTTGCT ATGAAGTGCA GAAGCTTCTT
    CTCCTGCTGA TGTCAAGCCC CGCCCCTAAC CAACAGTCCC GCCCACTATC CATCACCCGC
    AGCGGGCAGG CTCTGCATGA GCAGGAGCGC CAGCGGGAGG CTGAGCGCCG CCGGGAAGAG
    CTGGCGGATG TGGCTCGTCT GCGGGGAGCC CTGGTACGAG AGCGCCAGCA GTGGGAGAGG
    GAGTGCCAGG CGCGGCAGCT TCAGCACTCA GAACTGGAGA CCACGCTTGA GCAGAGGGAG
    CTCTTGTGCC ACCTAGAGGA GCGGAGACTG CAGAGCGAGC GGCAGGAGCT CCAGGAGCAG
    CTGCAGGAGT ACCAACAGAG TCTGGAGAGG CTCAGAGAAG GCCAGAGGAA TGTGGAGAAG
    GAGAAGGAGA AACTGGAGAC TCAGAAGATG CTGCTGGAGG GCTTGAAGCA CGGGTGCCAG
    CAGAACCTTC CAGCTATGGT CATACCACTG GACGGACAGC AGACACAGAA TCACCTGGGC
    ATTCAGGACG AGCATGGCTC TATTTTTGTC AATGAGGCAG CGTTCATATC TTCTTCTGTA
    AATAACAGGC ACCTCCATCT TCAGCATCAT CCACAGCATC ATCACCAGAC TGTCCATGTG
    CACTCAGGAT CTATAGGATA CTCAGATTCT CCCAGCGCTC AGAACAGTCT TAATTCACTG
    CTAGCCCGAT CCAACCACAG ACCCAGCTCT GCTGGAGCGT CCGACCACAG CTGGACGTCT
    CTGAGGAGTC TGACAGAGAG TGCAAACAAC AATGTGGGTC AGATCAGCCA TGTTGAAGAG
    TCTTGGTCAG CAGGGGTAAC GGGAGCGGGA CTCCATCAAA CTGATGTCCT GCTCGTCAGC
    CAGCCCATCC TAAGCATGGA GACAGAGAAC AGGGATACTG ATGGAGAGGA GACCATCGTG
    TATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211762.2
    CDS310..4638
    Translation

    Target ORF information:

    RefSeq Version XM_017356273.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356273.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCTG ACTCAGACTC TCCGTTTAAC TACTCGTGGC CATCCTTTCC AAAGATGAGA 
    ATCCGGAGAC GGACATCAAA AGCAGAGAAG CAGTTTTCGT CTCCGGGCTC CTGCACTCCG
    TTTCACCTTC ATTCCTCAGC CGCTGCAGCC GTCAGGCTCA CTGCCATGCT CAAAGGCTCC
    ACTAAGGACA GTGAGCTGAA TATGAAGTAC GCCGTTCCAG GTGCCACAGA GGACAGCTCT
    GGGACCGATA GTGGCATGTG GCGGATGTCA GATACGGGGG TCTCATCACC AGGGACAATA
    GATATATCTC CTGCTGTTAA AGATGATGAA GGGCAGAGAG GACGGCACCC CGCCTTTCGT
    TCTTTCTCTC CCACCTCTCC AGCAGGACTG ATGCTGGATA GATTCATGCG GGCCACGAGA
    ACCACTGAAC TGACCTACAA CAGCTCAAGC TCACCTACAG AGTCATGTGA CCTCAGAAGT
    CCAATCCTCT CTCTAAGCCC GAGTCTGGTT GCCTTGGAAA TGGACAGCGA GGAGGATGAC
    GATGATGACA TTTTAATGAA GAAGCCCCTC CCACAACTGC AACCGGATTC CAAAGACAGC
    ACAGATGAGC AGGTTATGTC CAGCCCCGCA CCTGTACTCA TCTGCACGCG CTCTCACTCT
    GCCTCCTCTG CCTGTAACCC CGCCCCTGCA AAGGATGTTG AGCAGGGTGT CCGACTCCGA
    TCCTATTCCT ACACCTCCCC CAAAATCAGC CTTCTGCCTC CACGTTTCAG CCGAGACGCC
    CATCCTCCAC CCGCCGACCT CAGCCAGGAT GGCGTTCTCT CCAGCAGCAG TGGCAGCAAG
    TCTCTTCTAC ACGCCCTGTC TCTGTCTAAA TCTCTGTCTC TGCTCAACCC TGTTAAGCAC
    CGCTCTTCCA GCACATCAGA GCAGCCTCAA GAGAAAAGGG AGTTAAGGTT TCGCAGGCGA
    GCTCAGTCGG CTGATGATGA AAGCAGTGCA GAGCTGGCCG ACTCGCTCCA ACATCTCACG
    CTCTCCGAGT TCCTCAAAGA GATTGAGGAA GAAGAATGGG ATAAATATAT CATCCCTTCC
    AAAACAGAGT CAGAGAAGTA CAAAGTCAGC CGCACATTCA GCTTCATAAA GAGCCGAATG
    TATAGCACTC GCAACAAGAA CAAGGGGAAA GGAAAAGAGA AAGAGACCAA AGACAAGCAG
    GTCAACGGAC ACCAGTTCAC AGCGGGATCT GTCCTGGGGT CGACACTCTG TGAGATCTGC
    AGCAAGCCAG CTTCAGGGAA AGAGGTTGTC CATTGCACAC ACTGTGCAGT GAGTGTCCAT
    AAGGGCTGTA AGGACTCATC TACGCCATGC CTGAAGAAAC CTCAAGACAA ATATTCAGTG
    ATGACAGTAA AAAGCAAGAC AGCCTCTCTT CCTCAAAATT TTATTGTGCG TGAGAGTTCA
    TCGTCGTCCC TTCAGATCTC TACCTCTGCT TCTCTCCCTG TCATGACATC ACGAGACTCG
    GCTCCTCTTC CTTGTCCTCT GTCCAGAAGT GTGCCCTTAA CCACTGAAAG GTTTAGCGAG
    AGCCCAGATG CTGATAGTGA TTCTCCTCTT TGGAGGAACC ACATCCAATC AGAGGAGCTT
    CTCCAAACCA TGGAGTCCTC AACATCCACA GACTCGTCCA TCATTGAAGA CTCGGTTGAT
    TCCCCTTTAC AGGTTGAGCT GCTGATGAAT GCTGTAGATT TGGAGGCAGA GTCATGGAGT
    TTAGCTGTGG AGGCCCAGTT CTGTAGGATG CAAGAAAAAC GCATCATCAA GAGACAGGAT
    GTGATTTACG AGTTCATGCA GACCGAGCTC CATCACGTGC AGACACTCAT GGTGATGGCA
    GAGGTCTTCA GAAGGGGAAT GCGGGATGAA GTGGGTCTGG ACGCAGAGAC CATTGGCCGG
    ATTTTTCCTT GCTTAGATGA GCTGCTCCAA CTCCATCGTG ATTTTCTGAC AGCAATGAGG
    GAACGAAGGC AAAGCTGCGT CCAGAACAGC AGCAGCAAAA ACTTTCTCAT CCACCGTGTG
    GGTGATATTT TTCTGCAACA GTTCTCCCAG GAGAACGCAG AGAAGATGAA GCAGGTGTAT
    GGAGAGTTTT GCAGTCATCA CACAGAAGCA GTCAGTTACT TCAAAGAGCT TCAGCAGCAG
    AACAAGAGAT TTCAGCTCTT CATCAAACAA CAGAGTAGTA ACTCACTGGT GAAGAGGCGA
    GAGATCCCAG AATGTATTTT GTTAGTGACG CAGCGAATTA CAAAGTACCC TGTCCTCCTA
    GAGAGAATAC TCCAGTACAC AGACGAGGGC ACAGAAGAGC ACTCTGACCT CTCTCGAGCT
    CTAGTCCTGA TCCGAGAGCT GATCTCCGCT GTGGACCAAT ATGTGAGTCA GTATGAGCAG
    AACCAGAAGC TGAGTGAAGT CTTGGGCCGC ATGGAGAACA AGTGCTCTAC CAAACTGAAG
    ACCGGCTGCA CTTTCCGCAA GCAGGACATC ACCAGCGGCA GGAATCTGCT GCACCAGGGC
    CCGCTGCTCT GGAAGACAGC CACTGGAAGA CTGAAAGATG TTCTGGCATT TTTGCTGTCT
    GATTCACTGG TGTTCCTTCA GGAGAAAGAT CAGAAATACA TTTTTGCTAC TGTGGACCAG
    AAGCCTCCAG TGATCTCTCT GCAGAAACTA ATTGTGCGTG AGGTGGCCAA TGAAGAGAGA
    GGCATGTTTC TGATCAGTGC ATCTTCAGCC GGGCCTGAGA TGTACGAGGT GCACGCAGCC
    TCTAAAGATG AACGCAACGC CTGGATGAGA CTCATCAGAG AGGCCGTGGA GAATTGCCCT
    GAAGAGGAGG ATGAAACTAC AAGTGAGTCA GAAGAGGAAA GGAGAGTCAC AGAAGCAAAG
    GTCCAAAAGA TCCACCGGCT GCAAGAGACG CTGTGTGGCC ATGATCACCT CATCTGTTCC
    AATCTGGAGG AGAAGCTGCA CATCTATGCC GAGCTGTCAG TGATGGCAGG CCAGCGAGAG
    GCAGGACCAG AGCCCAGGCT GCTGGTACGA CCCAGCACTG AAGACGTTCC CCAGGCTGGA
    GTACTACTCA GCGCCGCTCT CAGAGAGGCG GAGAGCCTAA GAGCAGCTCT CTCCTCTCAT
    TGTGCTTCCA CTCCTCCCGT CCCGTCCGGT CCAGAAGATC TAAACAAACC TGAACCCTGC
    ACTCCTCCAT CTCCTCCTCT GCCTCCTGAA AGTCCACTGA CAGACCCCAT CCAGAGTGAG
    GAGGAGGAGT GGAGAACTGA GGAAAGCTCA GAAGCACAGA CCTTTAATCA CAACTCGGAC
    TCTGACCACA ATCTACAGCT GAAGGTGAGT CAGAGTGTGC AGAGCTTGAC TCAGCTGCTG
    TACAGTCTAC AGGCTGCAGT TACCATCCAA GACAGTTGCT ATGAAGTGCA GAAGCTTCTT
    CTCCTGCTGA TGTCAAGCCC CGCCCCTAAC CAACAGTCCC GCCCACTATC CATCACCCGC
    AGCGGGCAGG CTCTGCATGA GCAGGAGCGC CAGCGGGAGG CTGAGCGCCG CCGGGAAGAG
    CTGGCGGATG TGGCTCGTCT GCGGGGAGCC CTGGTACGAG AGCGCCAGCA GTGGGAGAGG
    GAGTGCCAGG CGCGGCAGCT TCAGCACTCA GAACTGGAGA CCACGCTTGA GCAGAGGGAG
    CTCTTGTGCC ACCTAGAGGA GCGGAGACTG CAGAGCGAGC GGCAGGAGCT CCAGGAGCAG
    CTGCAGGAGT ACCAACAGAG TCTGGAGAGG CTCAGAGAAG GCCAGAGGAA TGTGGAGAAG
    GAGAAGGAGA AACTGGAGAC TCAGAAGATG CTGCTGGAGG GCTTGAAGCA CGGGTGCCAG
    CAGAACCTTC CAGCTATGGT CATACCACTG GACGGACAGC AGACACAGAA TCACCTGGGC
    ATTCAGGACG AGCATGGCTC TATTTTTGTC AATGAGGCAG CGTTCATATC TTCTTCTGTA
    AATAACAGGC ACCTCCATCT TCAGCATCAT CCACAGCATC ATCACCAGAC TGTCCATGTG
    CACTCAGGAT CTATAGGATA CTCAGATTCT CCCAGCGCTC AGAACAGTCT TAATTCACTG
    CTAGCCCGAT CCAACCACAG ACCCAGCTCT GCTGGAGCGT CCGACCACAG CTGGACGTCT
    CTGAGGAGTC TGACAGAGAG TGCAAACAAC AATGTGGGTC AGATCAGCCA TGTTGAAGAG
    TCTTGGTCAG CAGGGGTAAC GGGAGCGGGA CTCCATCAAA CTGATGTCCT GCTCGTCAGC
    CAGCCCATCC TAAGCATGGA GACAGAGAAC AGGGATACTG ATGGAGAGGA GACCATCGTG
    TATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59223
    Clone ID Related Accession (Same CDS sequence) XM_017356273.1 , XM_021476535.1
    Accession Version XM_017356273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCTG ACTCAGACTC TCCGTTTAAC TACTCGTGGC CATCCTTTCC AAAGATGAGA 
    ATCCGGAGAC GGACATCAAA AGCAGAGAAG CAGTTTTCGT CTCCGGGCTC CTGCACTCCG
    TTTCACCTTC ATTCCTCAGC CGCTGCAGCC GTCAGGCTCA CTGCCATGCT CAAAGGCTCC
    ACTAAGGACA GTGAGCTGAA TATGAAGTAC GCCGTTCCAG GTGCCACAGA GGACAGCTCT
    GGGACCGATA GTGGCATGTG GCGGATGTCA GATACGGGGG TCTCATCACC AGGGACAATA
    GATATATCTC CAACTGTTAA AGATGATGAA GGGCAGAGAG GACGGTACCC CGCCTCTCGT
    TCTTTCTCTC CCACCTCTCC AGCAGGACTG ATGCTGGATA GATTCATGCG GGCCACGAGA
    ACCACTGAAC TGACCTACAA CAGCTCAAGC TCACCTACAG AGTCATGTGA CCTCAGAAGT
    CCAATCCTCT CTCTAAGCCC GAGTCTGGTT GCCTTGGAAA TGGACAGCGA GGAGGATGAC
    GATGATGACA TTTTAATGAA GAAGCCCCTC CCACAACTGC AACCGGATTC CAAAGACAGC
    ACAGATGAGC AGGTTATGTC CAGCCCCGCA CCTGTACTCA TCTGCACGCG CTCTCACTCT
    GCCTCCTCTG CCTGTAACCC CGCCCCTGCA AAGGATGTTG AGCAGGGTGT CCGACTCCGA
    TCCTATTCCT ACACCTCCCC CAAAATCAGC CTTCTGCCTC CACGTTTCAG CCGAGACGCC
    CATCCTCCAC CCGCCGACCT CAGCCAGGAT GGCGTTCTCT CCAGCAGCAG TGGCAGCAAG
    TCTCTTCTAC ACGCCCTGTC TCTGTCTAAA TCTCTGTCTC TGTTCAACCC TGTTAAGCAC
    CGCTCTTCCA GCACATCAGA ACAGCCTCAA GAGAAAAGGG AGTTAAGGTT TCGCAGGCGA
    GCTCAGTCGG CTGATGATGA AAGCAGTGCA GAGCTGGCCG ACTCGCTCCA ACATCTCACG
    CTCTCAGAGT TCCTCAAAGA GATTGAGGAA GAAGAATGGG ATAAATATAT CATCCCTTCC
    AAAACAGAGT CAGAAAAGTA CAAAGTCAGC CGCACATTCA GCTTCATAAA GAGCCGAATG
    TATAGCACTC GCAACAAGAA CAAGGGGAAA GGAAAAGAGA AAGAGACCAA AGACAAGCAG
    GTCAACGGAC ACCAGTTCAC AGCGGGATCT GTCCTGGGGT CGACACTCTG TGAGATCTGC
    AGCAAGCCAG CTTCAGGGAA AGAGGTTGTC CATTGCACAC ACTGTGCAGT GAGTGTCCAT
    AAGGGCTGTA AGGACTCATC TACGCCATGC CTGAAGAAAC CTCAAGACAA ATATTCAGTG
    ATGACAGTAA AAAGCAAGAC AGCCTCTCTT CCTCAAAATT TTATTGTGCG TGAGAGTTCA
    TCGTCGTCCC TTCAGATCTC TACCTCTGCT TCTCTCCCTG TGATGACATC ACGAGACTCG
    GCTCCTCTTC CTTGTCCTCT GTCCAGAAGT GTGCCCTTAA CCACTGAAAG GCTTAGCGAG
    AGCCCAGAAG CTGATAGTGA TTCTAATCTT TGGAGGAACC ACATCCAATC AGAGGAGCTT
    CTCCAAACCA TGGAGTCCTC AACATCCACA GACTCGTCCA TCATTGAAGA CTCGGTTGAT
    TCCCCTTTAC AGATTGAGCT GCTGATGAAT GCTGTAGATT TGGAGGCAGA GTCATGGAGT
    TTAGCTGTGG AGGCCCAGTT CTGTAGGATG CAAGAAAAAC GCATCATCAA GAGACAGGAT
    GTGATTTACG AGTTCATGCA GACCGAGCTC CATCACGTGC AGACACTCAT GGTGATGGCA
    GAGGTCTTCA GAAGGGGAAT GCGGGATGAA GTGGGTCTGG ACGCAGAGAC CATTGGCCGG
    ATTTTTCCTT GCTTAGATGA GCTACTCCAA CTCCATCGTG ATTTTCTGAC AGCAATGAGG
    GAACGAAGGC AAAGCTGCGT CCAGAACAAC AGCAGCAAAA ACTTTCTCAT CCACCGTGTG
    GGTGATATTT TTCTGCAACA GTTCTCCCAG GAGAACGCAG AGAAGATGAA GCAGGTGTAT
    GGAGAGTTTT GCAGTCATCA CACAGAAGCA GTCAGTTACT TCAAAGAGCT TCAGCAGCAG
    AACAAGAGAT TTCAGCTCTT CATCAAACAA CAGAGTAGTA ACTCGCTGGT GAAGAGGCGA
    GAGATCCCAG AATGTATTTT GTTAGTGACG CAGCGAATTA CAAAGTACCC TGTCCTCCTA
    GAGAGAATAC TCCAGTACAC AGACGAGGGC ACAGAAGAGC ACTCTGACCT CTCTCGAGCT
    CTAGTCCTGA TCCGAGAGCT GATCTCCGCT GTGGACCAAT ATGTGAGTCA GTATGAGCAG
    AACCAGAAGC TGAGTGAAGT CTTGGGCCGC ATGGAGAACA AGTGCTCTAC CAAACTGAAG
    ACCGGCTGCA CTTTCCGCAA GCAGGACATC ACCAGCGGCA GGAATCTGCT GCACCAGGGC
    CCGCTGCTCT GGAAGACAGC CACTGGAAGA CTGAAAGATG TTCTGGCATT TTTGCTGTCT
    GATTCACTGG TGTTCCTTCA GGAGAAAGAT CAGAAATACA TTTTTGCTAC TGTGGACCAG
    AAGCCTCCAG TGATCTCTTT GCAGAAACTA ATTGTGCGTG AGGTGGCCAA TGAAGAGAGA
    GGCATGTTTC TGATCAGTGC ATCTTCAGCC GGGCCTGAGA TGTACGAGGT GCATGCAGCC
    TCTAAAGATG AACGCAACGC CTGGATGAGA CTCATCAGAG AGGCCGTGGA GAATTGCCCT
    GAAGAGGAGG ATGAAACTAC AAGTGAGTCA GAAGAGGAAA GGAGAGTCAC AGAAGCAAAG
    GTCCAAAAGA TCCACCGGCT GCAAGAGACG CTGTGTGGCC ATGATCACCT CATCTGTTCC
    AATCTGGAGG AGAAGCTGCA CATCTATGCC GAGCTGTCAG TGATGGCAGG CCAGCGAGAG
    GCAGGACCAG AGCCCAGGCT GCTGGTACGA CCCAGCACTG AAGACGTTCC CCAGGCTGGA
    GTACTACTCA GCGCCGCTCT CAGAGAGGCG GAGAGCCTAA GAGCAGCTCT CTCCTCTCAT
    TGTGCTTCCA CTCCTCCCGT CCCGTCCGGT CCAGAAGATC TAAACAAACC TGAACCCTGC
    ACTCCTCCAT CTCCTCTGCC TCCTGAAAGT CCACTGACAG ACCCCATCCA GAGTGAGGAG
    GAGGAGTGGA GAACTGAGGA AAGCTCAGAA GCACAGACCT TTAATCACAA CTCGGACTCT
    GACCACAATC TACAGCTGAA GGTGAGTCAG AGTGTGCAGA GCTTGACTCA GCTGCTGTAC
    AGTCTACAGG CTGCAGTTAC CATCCAAGAC AGTTGCTATG AAGTGCAGAA GCTTCTTCTC
    CTGCTGATGT CAAGCCCCGC CCCTAACCAA CAGTCCCGCC CACTATCCAT CACCCGCAGC
    GGGCAGGCTC TGCATGAGCA GGAGCGCCAG CGGGAGGCCG AGCGCCGCCG GGAAGAGCTG
    GCGGATGTGG CTCGTCTGCG GGGAGCCCTG GTACGAGAGC GCCAGCAGTG GGAGAGGGAG
    TGCCAGGCGC GGCAGCTTCA GCACTCAGAA CTGGAGACCA CGCTTGAGCA GAGGGAGCTC
    TTGTGCCACC TAGAGGAGCG GAGGCTGCAG AGCGAGCGGC AGGAGCTCCA GGAGCAGCTG
    CAGGAATACC AACAGAGTCT GGAGAGGCTC AGAGAAGGCC AGAGGAATGT GGAGAAGGAG
    AAGGAGAAAC TGGAGACTCA GAAGATGCTG CTGGAGGGCT TGAAGCACGG GTGCCAGCAG
    AACCTTCCAG CTATGGTCAT ACCACTGGAC GGACAGCAGA CACAGAATCA CCTGGGCATT
    CAGGACGAGC ATGGCTCTAT TTTTGTCAAT GAGGCAGCGT TCATATCTTC TTCTGTAAAT
    AACAGGCACC TCCATCTTCA GCATCATCCA CAGCATCATC ACCAGACTGT CCATGTGCAC
    TCAGGATCTA TAGGATACTC AGATTCTCCC AGCGCTCAGA ACAGTCTTAA TTCACTGCTA
    GCCCGATCCA ACCACAGACC CAGCTCTGCT GGAGCATCCG ACCACAGCTG GACGTCTCTG
    AGGAGTCTGA CAGAGAGTGC AAACAACAAT GTGGGTCAGA TCAGCCATGT TGAAGAGTCT
    TGGTCAGCAG GGGTAACGGG AGCGGGACTC CATCAAACTG ATGTCCTGCT CGTCAGCCAG
    CCCTTCCTAA GCATGGAGAC AGAGAACAGA GATACTGATG GAGAGGAGAC CATCGTGTAT
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211762.1
    CDS295..4620
    Translation

    Target ORF information:

    RefSeq Version XM_017356273.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCTG ACTCAGACTC TCCGTTTAAC TACTCGTGGC CATCCTTTCC AAAGATGAGA 
    ATCCGGAGAC GGACATCAAA AGCAGAGAAG CAGTTTTCGT CTCCGGGCTC CTGCACTCCG
    TTTCACCTTC ATTCCTCAGC CGCTGCAGCC GTCAGGCTCA CTGCCATGCT CAAAGGCTCC
    ACTAAGGACA GTGAGCTGAA TATGAAGTAC GCCGTTCCAG GTGCCACAGA GGACAGCTCT
    GGGACCGATA GTGGCATGTG GCGGATGTCA GATACGGGGG TCTCATCACC AGGGACAATA
    GATATATCTC CAACTGTTAA AGATGATGAA GGGCAGAGAG GACGGTACCC CGCCTCTCGT
    TCTTTCTCTC CCACCTCTCC AGCAGGACTG ATGCTGGATA GATTCATGCG GGCCACGAGA
    ACCACTGAAC TGACCTACAA CAGCTCAAGC TCACCTACAG AGTCATGTGA CCTCAGAAGT
    CCAATCCTCT CTCTAAGCCC GAGTCTGGTT GCCTTGGAAA TGGACAGCGA GGAGGATGAC
    GATGATGACA TTTTAATGAA GAAGCCCCTC CCACAACTGC AACCGGATTC CAAAGACAGC
    ACAGATGAGC AGGTTATGTC CAGCCCCGCA CCTGTACTCA TCTGCACGCG CTCTCACTCT
    GCCTCCTCTG CCTGTAACCC CGCCCCTGCA AAGGATGTTG AGCAGGGTGT CCGACTCCGA
    TCCTATTCCT ACACCTCCCC CAAAATCAGC CTTCTGCCTC CACGTTTCAG CCGAGACGCC
    CATCCTCCAC CCGCCGACCT CAGCCAGGAT GGCGTTCTCT CCAGCAGCAG TGGCAGCAAG
    TCTCTTCTAC ACGCCCTGTC TCTGTCTAAA TCTCTGTCTC TGTTCAACCC TGTTAAGCAC
    CGCTCTTCCA GCACATCAGA ACAGCCTCAA GAGAAAAGGG AGTTAAGGTT TCGCAGGCGA
    GCTCAGTCGG CTGATGATGA AAGCAGTGCA GAGCTGGCCG ACTCGCTCCA ACATCTCACG
    CTCTCAGAGT TCCTCAAAGA GATTGAGGAA GAAGAATGGG ATAAATATAT CATCCCTTCC
    AAAACAGAGT CAGAAAAGTA CAAAGTCAGC CGCACATTCA GCTTCATAAA GAGCCGAATG
    TATAGCACTC GCAACAAGAA CAAGGGGAAA GGAAAAGAGA AAGAGACCAA AGACAAGCAG
    GTCAACGGAC ACCAGTTCAC AGCGGGATCT GTCCTGGGGT CGACACTCTG TGAGATCTGC
    AGCAAGCCAG CTTCAGGGAA AGAGGTTGTC CATTGCACAC ACTGTGCAGT GAGTGTCCAT
    AAGGGCTGTA AGGACTCATC TACGCCATGC CTGAAGAAAC CTCAAGACAA ATATTCAGTG
    ATGACAGTAA AAAGCAAGAC AGCCTCTCTT CCTCAAAATT TTATTGTGCG TGAGAGTTCA
    TCGTCGTCCC TTCAGATCTC TACCTCTGCT TCTCTCCCTG TGATGACATC ACGAGACTCG
    GCTCCTCTTC CTTGTCCTCT GTCCAGAAGT GTGCCCTTAA CCACTGAAAG GCTTAGCGAG
    AGCCCAGAAG CTGATAGTGA TTCTAATCTT TGGAGGAACC ACATCCAATC AGAGGAGCTT
    CTCCAAACCA TGGAGTCCTC AACATCCACA GACTCGTCCA TCATTGAAGA CTCGGTTGAT
    TCCCCTTTAC AGATTGAGCT GCTGATGAAT GCTGTAGATT TGGAGGCAGA GTCATGGAGT
    TTAGCTGTGG AGGCCCAGTT CTGTAGGATG CAAGAAAAAC GCATCATCAA GAGACAGGAT
    GTGATTTACG AGTTCATGCA GACCGAGCTC CATCACGTGC AGACACTCAT GGTGATGGCA
    GAGGTCTTCA GAAGGGGAAT GCGGGATGAA GTGGGTCTGG ACGCAGAGAC CATTGGCCGG
    ATTTTTCCTT GCTTAGATGA GCTACTCCAA CTCCATCGTG ATTTTCTGAC AGCAATGAGG
    GAACGAAGGC AAAGCTGCGT CCAGAACAAC AGCAGCAAAA ACTTTCTCAT CCACCGTGTG
    GGTGATATTT TTCTGCAACA GTTCTCCCAG GAGAACGCAG AGAAGATGAA GCAGGTGTAT
    GGAGAGTTTT GCAGTCATCA CACAGAAGCA GTCAGTTACT TCAAAGAGCT TCAGCAGCAG
    AACAAGAGAT TTCAGCTCTT CATCAAACAA CAGAGTAGTA ACTCGCTGGT GAAGAGGCGA
    GAGATCCCAG AATGTATTTT GTTAGTGACG CAGCGAATTA CAAAGTACCC TGTCCTCCTA
    GAGAGAATAC TCCAGTACAC AGACGAGGGC ACAGAAGAGC ACTCTGACCT CTCTCGAGCT
    CTAGTCCTGA TCCGAGAGCT GATCTCCGCT GTGGACCAAT ATGTGAGTCA GTATGAGCAG
    AACCAGAAGC TGAGTGAAGT CTTGGGCCGC ATGGAGAACA AGTGCTCTAC CAAACTGAAG
    ACCGGCTGCA CTTTCCGCAA GCAGGACATC ACCAGCGGCA GGAATCTGCT GCACCAGGGC
    CCGCTGCTCT GGAAGACAGC CACTGGAAGA CTGAAAGATG TTCTGGCATT TTTGCTGTCT
    GATTCACTGG TGTTCCTTCA GGAGAAAGAT CAGAAATACA TTTTTGCTAC TGTGGACCAG
    AAGCCTCCAG TGATCTCTTT GCAGAAACTA ATTGTGCGTG AGGTGGCCAA TGAAGAGAGA
    GGCATGTTTC TGATCAGTGC ATCTTCAGCC GGGCCTGAGA TGTACGAGGT GCATGCAGCC
    TCTAAAGATG AACGCAACGC CTGGATGAGA CTCATCAGAG AGGCCGTGGA GAATTGCCCT
    GAAGAGGAGG ATGAAACTAC AAGTGAGTCA GAAGAGGAAA GGAGAGTCAC AGAAGCAAAG
    GTCCAAAAGA TCCACCGGCT GCAAGAGACG CTGTGTGGCC ATGATCACCT CATCTGTTCC
    AATCTGGAGG AGAAGCTGCA CATCTATGCC GAGCTGTCAG TGATGGCAGG CCAGCGAGAG
    GCAGGACCAG AGCCCAGGCT GCTGGTACGA CCCAGCACTG AAGACGTTCC CCAGGCTGGA
    GTACTACTCA GCGCCGCTCT CAGAGAGGCG GAGAGCCTAA GAGCAGCTCT CTCCTCTCAT
    TGTGCTTCCA CTCCTCCCGT CCCGTCCGGT CCAGAAGATC TAAACAAACC TGAACCCTGC
    ACTCCTCCAT CTCCTCTGCC TCCTGAAAGT CCACTGACAG ACCCCATCCA GAGTGAGGAG
    GAGGAGTGGA GAACTGAGGA AAGCTCAGAA GCACAGACCT TTAATCACAA CTCGGACTCT
    GACCACAATC TACAGCTGAA GGTGAGTCAG AGTGTGCAGA GCTTGACTCA GCTGCTGTAC
    AGTCTACAGG CTGCAGTTAC CATCCAAGAC AGTTGCTATG AAGTGCAGAA GCTTCTTCTC
    CTGCTGATGT CAAGCCCCGC CCCTAACCAA CAGTCCCGCC CACTATCCAT CACCCGCAGC
    GGGCAGGCTC TGCATGAGCA GGAGCGCCAG CGGGAGGCCG AGCGCCGCCG GGAAGAGCTG
    GCGGATGTGG CTCGTCTGCG GGGAGCCCTG GTACGAGAGC GCCAGCAGTG GGAGAGGGAG
    TGCCAGGCGC GGCAGCTTCA GCACTCAGAA CTGGAGACCA CGCTTGAGCA GAGGGAGCTC
    TTGTGCCACC TAGAGGAGCG GAGGCTGCAG AGCGAGCGGC AGGAGCTCCA GGAGCAGCTG
    CAGGAATACC AACAGAGTCT GGAGAGGCTC AGAGAAGGCC AGAGGAATGT GGAGAAGGAG
    AAGGAGAAAC TGGAGACTCA GAAGATGCTG CTGGAGGGCT TGAAGCACGG GTGCCAGCAG
    AACCTTCCAG CTATGGTCAT ACCACTGGAC GGACAGCAGA CACAGAATCA CCTGGGCATT
    CAGGACGAGC ATGGCTCTAT TTTTGTCAAT GAGGCAGCGT TCATATCTTC TTCTGTAAAT
    AACAGGCACC TCCATCTTCA GCATCATCCA CAGCATCATC ACCAGACTGT CCATGTGCAC
    TCAGGATCTA TAGGATACTC AGATTCTCCC AGCGCTCAGA ACAGTCTTAA TTCACTGCTA
    GCCCGATCCA ACCACAGACC CAGCTCTGCT GGAGCATCCG ACCACAGCTG GACGTCTCTG
    AGGAGTCTGA CAGAGAGTGC AAACAACAAT GTGGGTCAGA TCAGCCATGT TGAAGAGTCT
    TGGTCAGCAG GGGTAACGGG AGCGGGACTC CATCAAACTG ATGTCCTGCT CGTCAGCCAG
    CCCTTCCTAA GCATGGAGAC AGAGAACAGA GATACTGATG GAGAGGAGAC CATCGTGTAT
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59223
    Clone ID Related Accession (Same CDS sequence) XM_017356273.1 , XM_021476535.1
    Accession Version XM_021476535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCTG ACTCAGACTC TCCGTTTAAC TACTCGTGGC CATCCTTTCC AAAGATGAGA 
    ATCCGGAGAC GGACATCAAA AGCAGAGAAG CAGTTTTCGT CTCCGGGCTC CTGCACTCCG
    TTTCACCTTC ATTCCTCAGC CGCTGCAGCC GTCAGGCTCA CTGCCATGCT CAAAGGCTCC
    ACTAAGGACA GTGAGCTGAA TATGAAGTAC GCCGTTCCAG GTGCCACAGA GGACAGCTCT
    GGGACCGATA GTGGCATGTG GCGGATGTCA GATACGGGGG TCTCATCACC AGGGACAATA
    GATATATCTC CAACTGTTAA AGATGATGAA GGGCAGAGAG GACGGTACCC CGCCTCTCGT
    TCTTTCTCTC CCACCTCTCC AGCAGGACTG ATGCTGGATA GATTCATGCG GGCCACGAGA
    ACCACTGAAC TGACCTACAA CAGCTCAAGC TCACCTACAG AGTCATGTGA CCTCAGAAGT
    CCAATCCTCT CTCTAAGCCC GAGTCTGGTT GCCTTGGAAA TGGACAGCGA GGAGGATGAC
    GATGATGACA TTTTAATGAA GAAGCCCCTC CCACAACTGC AACCGGATTC CAAAGACAGC
    ACAGATGAGC AGGTTATGTC CAGCCCCGCA CCTGTACTCA TCTGCACGCG CTCTCACTCT
    GCCTCCTCTG CCTGTAACCC CGCCCCTGCA AAGGATGTTG AGCAGGGTGT CCGACTCCGA
    TCCTATTCCT ACACCTCCCC CAAAATCAGC CTTCTGCCTC CACGTTTCAG CCGAGACGCC
    CATCCTCCAC CCGCCGACCT CAGCCAGGAT GGCGTTCTCT CCAGCAGCAG TGGCAGCAAG
    TCTCTTCTAC ACGCCCTGTC TCTGTCTAAA TCTCTGTCTC TGTTCAACCC TGTTAAGCAC
    CGCTCTTCCA GCACATCAGA ACAGCCTCAA GAGAAAAGGG AGTTAAGGTT TCGCAGGCGA
    GCTCAGTCGG CTGATGATGA AAGCAGTGCA GAGCTGGCCG ACTCGCTCCA ACATCTCACG
    CTCTCAGAGT TCCTCAAAGA GATTGAGGAA GAAGAATGGG ATAAATATAT CATCCCTTCC
    AAAACAGAGT CAGAAAAGTA CAAAGTCAGC CGCACATTCA GCTTCATAAA GAGCCGAATG
    TATAGCACTC GCAACAAGAA CAAGGGGAAA GGAAAAGAGA AAGAGACCAA AGACAAGCAG
    GTCAACGGAC ACCAGTTCAC AGCGGGATCT GTCCTGGGGT CGACACTCTG TGAGATCTGC
    AGCAAGCCAG CTTCAGGGAA AGAGGTTGTC CATTGCACAC ACTGTGCAGT GAGTGTCCAT
    AAGGGCTGTA AGGACTCATC TACGCCATGC CTGAAGAAAC CTCAAGACAA ATATTCAGTG
    ATGACAGTAA AAAGCAAGAC AGCCTCTCTT CCTCAAAATT TTATTGTGCG TGAGAGTTCA
    TCGTCGTCCC TTCAGATCTC TACCTCTGCT TCTCTCCCTG TGATGACATC ACGAGACTCG
    GCTCCTCTTC CTTGTCCTCT GTCCAGAAGT GTGCCCTTAA CCACTGAAAG GCTTAGCGAG
    AGCCCAGAAG CTGATAGTGA TTCTAATCTT TGGAGGAACC ACATCCAATC AGAGGAGCTT
    CTCCAAACCA TGGAGTCCTC AACATCCACA GACTCGTCCA TCATTGAAGA CTCGGTTGAT
    TCCCCTTTAC AGATTGAGCT GCTGATGAAT GCTGTAGATT TGGAGGCAGA GTCATGGAGT
    TTAGCTGTGG AGGCCCAGTT CTGTAGGATG CAAGAAAAAC GCATCATCAA GAGACAGGAT
    GTGATTTACG AGTTCATGCA GACCGAGCTC CATCACGTGC AGACACTCAT GGTGATGGCA
    GAGGTCTTCA GAAGGGGAAT GCGGGATGAA GTGGGTCTGG ACGCAGAGAC CATTGGCCGG
    ATTTTTCCTT GCTTAGATGA GCTACTCCAA CTCCATCGTG ATTTTCTGAC AGCAATGAGG
    GAACGAAGGC AAAGCTGCGT CCAGAACAAC AGCAGCAAAA ACTTTCTCAT CCACCGTGTG
    GGTGATATTT TTCTGCAACA GTTCTCCCAG GAGAACGCAG AGAAGATGAA GCAGGTGTAT
    GGAGAGTTTT GCAGTCATCA CACAGAAGCA GTCAGTTACT TCAAAGAGCT TCAGCAGCAG
    AACAAGAGAT TTCAGCTCTT CATCAAACAA CAGAGTAGTA ACTCGCTGGT GAAGAGGCGA
    GAGATCCCAG AATGTATTTT GTTAGTGACG CAGCGAATTA CAAAGTACCC TGTCCTCCTA
    GAGAGAATAC TCCAGTACAC AGACGAGGGC ACAGAAGAGC ACTCTGACCT CTCTCGAGCT
    CTAGTCCTGA TCCGAGAGCT GATCTCCGCT GTGGACCAAT ATGTGAGTCA GTATGAGCAG
    AACCAGAAGC TGAGTGAAGT CTTGGGCCGC ATGGAGAACA AGTGCTCTAC CAAACTGAAG
    ACCGGCTGCA CTTTCCGCAA GCAGGACATC ACCAGCGGCA GGAATCTGCT GCACCAGGGC
    CCGCTGCTCT GGAAGACAGC CACTGGAAGA CTGAAAGATG TTCTGGCATT TTTGCTGTCT
    GATTCACTGG TGTTCCTTCA GGAGAAAGAT CAGAAATACA TTTTTGCTAC TGTGGACCAG
    AAGCCTCCAG TGATCTCTTT GCAGAAACTA ATTGTGCGTG AGGTGGCCAA TGAAGAGAGA
    GGCATGTTTC TGATCAGTGC ATCTTCAGCC GGGCCTGAGA TGTACGAGGT GCATGCAGCC
    TCTAAAGATG AACGCAACGC CTGGATGAGA CTCATCAGAG AGGCCGTGGA GAATTGCCCT
    GAAGAGGAGG ATGAAACTAC AAGTGAGTCA GAAGAGGAAA GGAGAGTCAC AGAAGCAAAG
    GTCCAAAAGA TCCACCGGCT GCAAGAGACG CTGTGTGGCC ATGATCACCT CATCTGTTCC
    AATCTGGAGG AGAAGCTGCA CATCTATGCC GAGCTGTCAG TGATGGCAGG CCAGCGAGAG
    GCAGGACCAG AGCCCAGGCT GCTGGTACGA CCCAGCACTG AAGACGTTCC CCAGGCTGGA
    GTACTACTCA GCGCCGCTCT CAGAGAGGCG GAGAGCCTAA GAGCAGCTCT CTCCTCTCAT
    TGTGCTTCCA CTCCTCCCGT CCCGTCCGGT CCAGAAGATC TAAACAAACC TGAACCCTGC
    ACTCCTCCAT CTCCTCTGCC TCCTGAAAGT CCACTGACAG ACCCCATCCA GAGTGAGGAG
    GAGGAGTGGA GAACTGAGGA AAGCTCAGAA GCACAGACCT TTAATCACAA CTCGGACTCT
    GACCACAATC TACAGCTGAA GGTGAGTCAG AGTGTGCAGA GCTTGACTCA GCTGCTGTAC
    AGTCTACAGG CTGCAGTTAC CATCCAAGAC AGTTGCTATG AAGTGCAGAA GCTTCTTCTC
    CTGCTGATGT CAAGCCCCGC CCCTAACCAA CAGTCCCGCC CACTATCCAT CACCCGCAGC
    GGGCAGGCTC TGCATGAGCA GGAGCGCCAG CGGGAGGCCG AGCGCCGCCG GGAAGAGCTG
    GCGGATGTGG CTCGTCTGCG GGGAGCCCTG GTACGAGAGC GCCAGCAGTG GGAGAGGGAG
    TGCCAGGCGC GGCAGCTTCA GCACTCAGAA CTGGAGACCA CGCTTGAGCA GAGGGAGCTC
    TTGTGCCACC TAGAGGAGCG GAGGCTGCAG AGCGAGCGGC AGGAGCTCCA GGAGCAGCTG
    CAGGAATACC AACAGAGTCT GGAGAGGCTC AGAGAAGGCC AGAGGAATGT GGAGAAGGAG
    AAGGAGAAAC TGGAGACTCA GAAGATGCTG CTGGAGGGCT TGAAGCACGG GTGCCAGCAG
    AACCTTCCAG CTATGGTCAT ACCACTGGAC GGACAGCAGA CACAGAATCA CCTGGGCATT
    CAGGACGAGC ATGGCTCTAT TTTTGTCAAT GAGGCAGCGT TCATATCTTC TTCTGTAAAT
    AACAGGCACC TCCATCTTCA GCATCATCCA CAGCATCATC ACCAGACTGT CCATGTGCAC
    TCAGGATCTA TAGGATACTC AGATTCTCCC AGCGCTCAGA ACAGTCTTAA TTCACTGCTA
    GCCCGATCCA ACCACAGACC CAGCTCTGCT GGAGCATCCG ACCACAGCTG GACGTCTCTG
    AGGAGTCTGA CAGAGAGTGC AAACAACAAT GTGGGTCAGA TCAGCCATGT TGAAGAGTCT
    TGGTCAGCAG GGGTAACGGG AGCGGGACTC CATCAAACTG ATGTCCTGCT CGTCAGCCAG
    CCCTTCCTAA GCATGGAGAC AGAGAACAGA GATACTGATG GAGAGGAGAC CATCGTGTAT
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332210.1
    CDS1024..5349
    Translation

    Target ORF information:

    RefSeq Version XM_021476535.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCTG ACTCAGACTC TCCGTTTAAC TACTCGTGGC CATCCTTTCC AAAGATGAGA 
    ATCCGGAGAC GGACATCAAA AGCAGAGAAG CAGTTTTCGT CTCCGGGCTC CTGCACTCCG
    TTTCACCTTC ATTCCTCAGC CGCTGCAGCC GTCAGGCTCA CTGCCATGCT CAAAGGCTCC
    ACTAAGGACA GTGAGCTGAA TATGAAGTAC GCCGTTCCAG GTGCCACAGA GGACAGCTCT
    GGGACCGATA GTGGCATGTG GCGGATGTCA GATACGGGGG TCTCATCACC AGGGACAATA
    GATATATCTC CAACTGTTAA AGATGATGAA GGGCAGAGAG GACGGTACCC CGCCTCTCGT
    TCTTTCTCTC CCACCTCTCC AGCAGGACTG ATGCTGGATA GATTCATGCG GGCCACGAGA
    ACCACTGAAC TGACCTACAA CAGCTCAAGC TCACCTACAG AGTCATGTGA CCTCAGAAGT
    CCAATCCTCT CTCTAAGCCC GAGTCTGGTT GCCTTGGAAA TGGACAGCGA GGAGGATGAC
    GATGATGACA TTTTAATGAA GAAGCCCCTC CCACAACTGC AACCGGATTC CAAAGACAGC
    ACAGATGAGC AGGTTATGTC CAGCCCCGCA CCTGTACTCA TCTGCACGCG CTCTCACTCT
    GCCTCCTCTG CCTGTAACCC CGCCCCTGCA AAGGATGTTG AGCAGGGTGT CCGACTCCGA
    TCCTATTCCT ACACCTCCCC CAAAATCAGC CTTCTGCCTC CACGTTTCAG CCGAGACGCC
    CATCCTCCAC CCGCCGACCT CAGCCAGGAT GGCGTTCTCT CCAGCAGCAG TGGCAGCAAG
    TCTCTTCTAC ACGCCCTGTC TCTGTCTAAA TCTCTGTCTC TGTTCAACCC TGTTAAGCAC
    CGCTCTTCCA GCACATCAGA ACAGCCTCAA GAGAAAAGGG AGTTAAGGTT TCGCAGGCGA
    GCTCAGTCGG CTGATGATGA AAGCAGTGCA GAGCTGGCCG ACTCGCTCCA ACATCTCACG
    CTCTCAGAGT TCCTCAAAGA GATTGAGGAA GAAGAATGGG ATAAATATAT CATCCCTTCC
    AAAACAGAGT CAGAAAAGTA CAAAGTCAGC CGCACATTCA GCTTCATAAA GAGCCGAATG
    TATAGCACTC GCAACAAGAA CAAGGGGAAA GGAAAAGAGA AAGAGACCAA AGACAAGCAG
    GTCAACGGAC ACCAGTTCAC AGCGGGATCT GTCCTGGGGT CGACACTCTG TGAGATCTGC
    AGCAAGCCAG CTTCAGGGAA AGAGGTTGTC CATTGCACAC ACTGTGCAGT GAGTGTCCAT
    AAGGGCTGTA AGGACTCATC TACGCCATGC CTGAAGAAAC CTCAAGACAA ATATTCAGTG
    ATGACAGTAA AAAGCAAGAC AGCCTCTCTT CCTCAAAATT TTATTGTGCG TGAGAGTTCA
    TCGTCGTCCC TTCAGATCTC TACCTCTGCT TCTCTCCCTG TGATGACATC ACGAGACTCG
    GCTCCTCTTC CTTGTCCTCT GTCCAGAAGT GTGCCCTTAA CCACTGAAAG GCTTAGCGAG
    AGCCCAGAAG CTGATAGTGA TTCTAATCTT TGGAGGAACC ACATCCAATC AGAGGAGCTT
    CTCCAAACCA TGGAGTCCTC AACATCCACA GACTCGTCCA TCATTGAAGA CTCGGTTGAT
    TCCCCTTTAC AGATTGAGCT GCTGATGAAT GCTGTAGATT TGGAGGCAGA GTCATGGAGT
    TTAGCTGTGG AGGCCCAGTT CTGTAGGATG CAAGAAAAAC GCATCATCAA GAGACAGGAT
    GTGATTTACG AGTTCATGCA GACCGAGCTC CATCACGTGC AGACACTCAT GGTGATGGCA
    GAGGTCTTCA GAAGGGGAAT GCGGGATGAA GTGGGTCTGG ACGCAGAGAC CATTGGCCGG
    ATTTTTCCTT GCTTAGATGA GCTACTCCAA CTCCATCGTG ATTTTCTGAC AGCAATGAGG
    GAACGAAGGC AAAGCTGCGT CCAGAACAAC AGCAGCAAAA ACTTTCTCAT CCACCGTGTG
    GGTGATATTT TTCTGCAACA GTTCTCCCAG GAGAACGCAG AGAAGATGAA GCAGGTGTAT
    GGAGAGTTTT GCAGTCATCA CACAGAAGCA GTCAGTTACT TCAAAGAGCT TCAGCAGCAG
    AACAAGAGAT TTCAGCTCTT CATCAAACAA CAGAGTAGTA ACTCGCTGGT GAAGAGGCGA
    GAGATCCCAG AATGTATTTT GTTAGTGACG CAGCGAATTA CAAAGTACCC TGTCCTCCTA
    GAGAGAATAC TCCAGTACAC AGACGAGGGC ACAGAAGAGC ACTCTGACCT CTCTCGAGCT
    CTAGTCCTGA TCCGAGAGCT GATCTCCGCT GTGGACCAAT ATGTGAGTCA GTATGAGCAG
    AACCAGAAGC TGAGTGAAGT CTTGGGCCGC ATGGAGAACA AGTGCTCTAC CAAACTGAAG
    ACCGGCTGCA CTTTCCGCAA GCAGGACATC ACCAGCGGCA GGAATCTGCT GCACCAGGGC
    CCGCTGCTCT GGAAGACAGC CACTGGAAGA CTGAAAGATG TTCTGGCATT TTTGCTGTCT
    GATTCACTGG TGTTCCTTCA GGAGAAAGAT CAGAAATACA TTTTTGCTAC TGTGGACCAG
    AAGCCTCCAG TGATCTCTTT GCAGAAACTA ATTGTGCGTG AGGTGGCCAA TGAAGAGAGA
    GGCATGTTTC TGATCAGTGC ATCTTCAGCC GGGCCTGAGA TGTACGAGGT GCATGCAGCC
    TCTAAAGATG AACGCAACGC CTGGATGAGA CTCATCAGAG AGGCCGTGGA GAATTGCCCT
    GAAGAGGAGG ATGAAACTAC AAGTGAGTCA GAAGAGGAAA GGAGAGTCAC AGAAGCAAAG
    GTCCAAAAGA TCCACCGGCT GCAAGAGACG CTGTGTGGCC ATGATCACCT CATCTGTTCC
    AATCTGGAGG AGAAGCTGCA CATCTATGCC GAGCTGTCAG TGATGGCAGG CCAGCGAGAG
    GCAGGACCAG AGCCCAGGCT GCTGGTACGA CCCAGCACTG AAGACGTTCC CCAGGCTGGA
    GTACTACTCA GCGCCGCTCT CAGAGAGGCG GAGAGCCTAA GAGCAGCTCT CTCCTCTCAT
    TGTGCTTCCA CTCCTCCCGT CCCGTCCGGT CCAGAAGATC TAAACAAACC TGAACCCTGC
    ACTCCTCCAT CTCCTCTGCC TCCTGAAAGT CCACTGACAG ACCCCATCCA GAGTGAGGAG
    GAGGAGTGGA GAACTGAGGA AAGCTCAGAA GCACAGACCT TTAATCACAA CTCGGACTCT
    GACCACAATC TACAGCTGAA GGTGAGTCAG AGTGTGCAGA GCTTGACTCA GCTGCTGTAC
    AGTCTACAGG CTGCAGTTAC CATCCAAGAC AGTTGCTATG AAGTGCAGAA GCTTCTTCTC
    CTGCTGATGT CAAGCCCCGC CCCTAACCAA CAGTCCCGCC CACTATCCAT CACCCGCAGC
    GGGCAGGCTC TGCATGAGCA GGAGCGCCAG CGGGAGGCCG AGCGCCGCCG GGAAGAGCTG
    GCGGATGTGG CTCGTCTGCG GGGAGCCCTG GTACGAGAGC GCCAGCAGTG GGAGAGGGAG
    TGCCAGGCGC GGCAGCTTCA GCACTCAGAA CTGGAGACCA CGCTTGAGCA GAGGGAGCTC
    TTGTGCCACC TAGAGGAGCG GAGGCTGCAG AGCGAGCGGC AGGAGCTCCA GGAGCAGCTG
    CAGGAATACC AACAGAGTCT GGAGAGGCTC AGAGAAGGCC AGAGGAATGT GGAGAAGGAG
    AAGGAGAAAC TGGAGACTCA GAAGATGCTG CTGGAGGGCT TGAAGCACGG GTGCCAGCAG
    AACCTTCCAG CTATGGTCAT ACCACTGGAC GGACAGCAGA CACAGAATCA CCTGGGCATT
    CAGGACGAGC ATGGCTCTAT TTTTGTCAAT GAGGCAGCGT TCATATCTTC TTCTGTAAAT
    AACAGGCACC TCCATCTTCA GCATCATCCA CAGCATCATC ACCAGACTGT CCATGTGCAC
    TCAGGATCTA TAGGATACTC AGATTCTCCC AGCGCTCAGA ACAGTCTTAA TTCACTGCTA
    GCCCGATCCA ACCACAGACC CAGCTCTGCT GGAGCATCCG ACCACAGCTG GACGTCTCTG
    AGGAGTCTGA CAGAGAGTGC AAACAACAAT GTGGGTCAGA TCAGCCATGT TGAAGAGTCT
    TGGTCAGCAG GGGTAACGGG AGCGGGACTC CATCAAACTG ATGTCCTGCT CGTCAGCCAG
    CCCTTCCTAA GCATGGAGAC AGAGAACAGA GATACTGATG GAGAGGAGAC CATCGTGTAT
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144575
    Clone ID Related Accession (Same CDS sequence) XM_017356269.2
    Accession Version XM_017356269.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394590.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGCTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAGCAGC CTCAAGAGAA AAGGGAGTTA AGGTTTCGCA GGCGAGCTCA GTCGGCTGAT
    GATGAAAGCA GTGCAGAGCT GGCCGACTCG CTCCAACATC TCACGCTCTC CGAGTTCCTC
    AAAGAGATTG AGGAAGAAGA ATGGGATAAA TATATCATCC CTTCCAAAAC AGAGTCAGAG
    AAGTACAAAG TCAGCCGCAC ATTCAGCTTC ATAAAGAGCC GAATGTATAG CACTCGCAAC
    AAGAACAAGG GGAAAGGAAA AGAGAAAGAG ACCAAAGACA AGCAGGTCAA CGGACACCAG
    TTCACAGCGG GATCTGTCCT GGGGTCGACA CTCTGTGAGA TCTGCAGCAA GCCAGCTTCA
    GGGAAAGAGG TTGTCCATTG CACACACTGT GCAGTGAGTG TCCATAAGGG CTGTAAGGAC
    TCATCTACGC CATGCCTGAA GAAACCTCAA GACAAATATT CAGTGATGAC AGTAAAAAGC
    AAGACAGCCT CTCTTCCTCA AAATTTTATT GTGCGTGAGA GTTCATCGTC GTCCCTTCAG
    ATCTCTACCT CTGCTTCTCT CCCTGTCATG ACATCACGAG ACTCGGCTCC TCTTCCTTGT
    CCTCTGTCCA GAAGTGTGCC CTTAACCACT GAAAGGTTTA GCGAGAGCCC AGATGCTGAT
    AGTGATTCTC CTCTTTGGAG GAACCACATC CAATCAGAGG AGCTTCTCCA AACCATGGAG
    TCCTCAACAT CCACAGACTC GTCCATCATT GAAGACTCGG TTGATTCCCC TTTACAGGTT
    GAGCTGCTGA TGAATGCTGT AGATTTGGAG GCAGAGTCAT GGAGTTTAGC TGTGGAGGCC
    CAGTTCTGTA GGATGCAAGA AAAACGCATC ATCAAGAGAC AGGATGTGAT TTACGAGTTC
    ATGCAGACCG AGCTCCATCA CGTGCAGACA CTCATGGTGA TGGCAGAGGT CTTCAGAAGG
    GGAATGCGGG ATGAAGTGGG TCTGGACGCA GAGACCATTG GCCGGATTTT TCCTTGCTTA
    GATGAGCTGC TCCAACTCCA TCGTGATTTT CTGACAGCAA TGAGGGAACG AAGGCAAAGC
    TGCGTCCAGA ACAGCAGCAG CAAAAACTTT CTCATCCACC GTGTGGGTGA TATTTTTCTG
    CAACAGTTCT CCCAGGAGAA CGCAGAGAAG ATGAAGCAGG TGTATGGAGA GTTTTGCAGT
    CATCACACAG AAGCAGTCAG TTACTTCAAA GAGCTTCAGC AGCAGAACAA GAGATTTCAG
    CTCTTCATCA AACAACAGAG TAGTAACTCA CTGGTGAAGA GGCGAGAGAT CCCAGAATGT
    ATTTTGTTAG TGACGCAGCG AATTACAAAG TACCCTGTCC TCCTAGAGAG AATACTCCAG
    TACACAGACG AGGGCACAGA AGAGCACTCT GACCTCTCTC GAGCTCTAGT CCTGATCCGA
    GAGCTGATCT CCGCTGTGGA CCAATATGTG AGTCAGTATG AGCAGAACCA GAAGCTGAGT
    GAAGTCTTGG GCCGCATGGA GAACAAGTGC TCTACCAAAC TGAAGACCGG CTGCACTTTC
    CGCAAGCAGG ACATCACCAG CGGCAGGAAT CTGCTGCACC AGGGCCCGCT GCTCTGGAAG
    ACAGCCACTG GAAGACTGAA AGATGTTCTG GCATTTTTGC TGTCTGATTC ACTGGTGTTC
    CTTCAGGAGA AAGATCAGAA ATACATTTTT GCTACTGTGG ACCAGAAGCC TCCAGTGATC
    TCTCTGCAGA AACTAATTGT GCGTGAGGTG GCCAATGAAG AGAGAGGCAT GTTTCTGATC
    AGTGCATCTT CAGCCGGGCC TGAGATGTAC GAGGTGCACG CAGCCTCTAA AGATGAACGC
    AACGCCTGGA TGAGACTCAT CAGAGAGGCC GTGGAGAATT GCCCTGAAGA GGAGGATGAA
    ACTACAAGTG AGTCAGAAGA GGAAAGGAGA GTCACAGAAG CAAAGGTCCA AAAGATCCAC
    CGGCTGCAAG AGACGCTGTG TGGCCATGAT CACCTCATCT GTTCCAATCT GGAGGAGAAG
    CTGCACATCT ATGCCGAGCT GTCAGTGATG GCAGGCCAGC GAGAGGCAGG ACCAGAGCCC
    AGGCTGCTGG TACGACCCAG CACTGAAGAC GTTCCCCAGG CTGGAGTACT ACTCAGCGCC
    GCTCTCAGAG AGGCGGAGAG CCTAAGAGCA GCTCTCTCCT CTCATTGTGC TTCCACTCCT
    CCCGTCCCGT CCGGTCCAGA AGATCTAAAC AAACCTGAAC CCTGCACTCC TCCATCTCCT
    CCTCTGCCTC CTGAAAGTCC ACTGACAGAC CCCATCCAGA GTGAGGAGGA GGAGTGGAGA
    ACTGAGGAAA GCTCAGAAGC ACAGACCTTT AATCACAACT CGGACTCTGA CCACAATCTA
    CAGCTGAAGG TGAGTCAGAG TGTGCAGAGC TTGACTCAGC TGCTGTACAG TCTACAGGCT
    GCAGTTACCA TCCAAGACAG TTGCTATGAA GTGCAGAAGC TTCTTCTCCT GCTGATGTCA
    AGCCCCGCCC CTAACCAACA GTCCCGCCCA CTATCCATCA CCCGCAGCGG GCAGGCTCTG
    CATGAGCAGG AGCGCCAGCG GGAGGCTGAG CGCCGCCGGG AAGAGCTGGC GGATGTGGCT
    CGTCTGCGGG GAGCCCTGGT ACGAGAGCGC CAGCAGTGGG AGAGGGAGTG CCAGGCGCGG
    CAGCTTCAGC ACTCAGAACT GGAGACCACG CTTGAGCAGA GGGAGCTCTT GTGCCACCTA
    GAGGAGCGGA GACTGCAGAG CGAGCGGCAG GAGCTCCAGG AGCAGCTGCA GGAGTACCAA
    CAGAGTCTGG AGAGGCTCAG AGAAGGCCAG AGGAATGTGG AGAAGGAGAA GGAGAAACTG
    GAGACTCAGA AGATGCTGCT GGAGGGCTTG AAGCACGGGT GCCAGCAGAA CCTTCCAGCT
    ATGGTCATAC CACTGGACGG ACAGCAGACA CAGAATCACC TGGGCATTCA GGACGAGCAT
    GGCTCTATTT TTGTCAATGA GGCAGCGTTC ATATCTTCTT CTGTAAATAA CAGGCACCTC
    CATCTTCAGC ATCATCCACA GCATCATCAC CAGACTGTCC ATGTGCACTC AGGATCTATA
    GGATACTCAG ATTCTCCCAG CGCTCAGAAC AGTCTTAATT CACTGCTAGC CCGATCCAAC
    CACAGACCCA GCTCTGCTGG AGCGTCCGAC CACAGCTGGA CGTCTCTGAG GAGTCTGACA
    GAGAGTGCAA ACAACAATGT GGGTCAGATC AGCCATGTTG AAGAGTCTTG GTCAGCAGGG
    GTAACGGGAG CGGGACTCCA TCAAACTGAT GTCCTGCTCG TCAGCCAGCC CATCCTAAGC
    ATGGAGACAG AGAACAGGGA TACTGATGGA GAGGAGACCA TCGTGTATCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211758.2
    CDS207..5660
    Translation

    Target ORF information:

    RefSeq Version XM_017356269.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356269.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGCTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAGCAGC CTCAAGAGAA AAGGGAGTTA AGGTTTCGCA GGCGAGCTCA GTCGGCTGAT
    GATGAAAGCA GTGCAGAGCT GGCCGACTCG CTCCAACATC TCACGCTCTC CGAGTTCCTC
    AAAGAGATTG AGGAAGAAGA ATGGGATAAA TATATCATCC CTTCCAAAAC AGAGTCAGAG
    AAGTACAAAG TCAGCCGCAC ATTCAGCTTC ATAAAGAGCC GAATGTATAG CACTCGCAAC
    AAGAACAAGG GGAAAGGAAA AGAGAAAGAG ACCAAAGACA AGCAGGTCAA CGGACACCAG
    TTCACAGCGG GATCTGTCCT GGGGTCGACA CTCTGTGAGA TCTGCAGCAA GCCAGCTTCA
    GGGAAAGAGG TTGTCCATTG CACACACTGT GCAGTGAGTG TCCATAAGGG CTGTAAGGAC
    TCATCTACGC CATGCCTGAA GAAACCTCAA GACAAATATT CAGTGATGAC AGTAAAAAGC
    AAGACAGCCT CTCTTCCTCA AAATTTTATT GTGCGTGAGA GTTCATCGTC GTCCCTTCAG
    ATCTCTACCT CTGCTTCTCT CCCTGTCATG ACATCACGAG ACTCGGCTCC TCTTCCTTGT
    CCTCTGTCCA GAAGTGTGCC CTTAACCACT GAAAGGTTTA GCGAGAGCCC AGATGCTGAT
    AGTGATTCTC CTCTTTGGAG GAACCACATC CAATCAGAGG AGCTTCTCCA AACCATGGAG
    TCCTCAACAT CCACAGACTC GTCCATCATT GAAGACTCGG TTGATTCCCC TTTACAGGTT
    GAGCTGCTGA TGAATGCTGT AGATTTGGAG GCAGAGTCAT GGAGTTTAGC TGTGGAGGCC
    CAGTTCTGTA GGATGCAAGA AAAACGCATC ATCAAGAGAC AGGATGTGAT TTACGAGTTC
    ATGCAGACCG AGCTCCATCA CGTGCAGACA CTCATGGTGA TGGCAGAGGT CTTCAGAAGG
    GGAATGCGGG ATGAAGTGGG TCTGGACGCA GAGACCATTG GCCGGATTTT TCCTTGCTTA
    GATGAGCTGC TCCAACTCCA TCGTGATTTT CTGACAGCAA TGAGGGAACG AAGGCAAAGC
    TGCGTCCAGA ACAGCAGCAG CAAAAACTTT CTCATCCACC GTGTGGGTGA TATTTTTCTG
    CAACAGTTCT CCCAGGAGAA CGCAGAGAAG ATGAAGCAGG TGTATGGAGA GTTTTGCAGT
    CATCACACAG AAGCAGTCAG TTACTTCAAA GAGCTTCAGC AGCAGAACAA GAGATTTCAG
    CTCTTCATCA AACAACAGAG TAGTAACTCA CTGGTGAAGA GGCGAGAGAT CCCAGAATGT
    ATTTTGTTAG TGACGCAGCG AATTACAAAG TACCCTGTCC TCCTAGAGAG AATACTCCAG
    TACACAGACG AGGGCACAGA AGAGCACTCT GACCTCTCTC GAGCTCTAGT CCTGATCCGA
    GAGCTGATCT CCGCTGTGGA CCAATATGTG AGTCAGTATG AGCAGAACCA GAAGCTGAGT
    GAAGTCTTGG GCCGCATGGA GAACAAGTGC TCTACCAAAC TGAAGACCGG CTGCACTTTC
    CGCAAGCAGG ACATCACCAG CGGCAGGAAT CTGCTGCACC AGGGCCCGCT GCTCTGGAAG
    ACAGCCACTG GAAGACTGAA AGATGTTCTG GCATTTTTGC TGTCTGATTC ACTGGTGTTC
    CTTCAGGAGA AAGATCAGAA ATACATTTTT GCTACTGTGG ACCAGAAGCC TCCAGTGATC
    TCTCTGCAGA AACTAATTGT GCGTGAGGTG GCCAATGAAG AGAGAGGCAT GTTTCTGATC
    AGTGCATCTT CAGCCGGGCC TGAGATGTAC GAGGTGCACG CAGCCTCTAA AGATGAACGC
    AACGCCTGGA TGAGACTCAT CAGAGAGGCC GTGGAGAATT GCCCTGAAGA GGAGGATGAA
    ACTACAAGTG AGTCAGAAGA GGAAAGGAGA GTCACAGAAG CAAAGGTCCA AAAGATCCAC
    CGGCTGCAAG AGACGCTGTG TGGCCATGAT CACCTCATCT GTTCCAATCT GGAGGAGAAG
    CTGCACATCT ATGCCGAGCT GTCAGTGATG GCAGGCCAGC GAGAGGCAGG ACCAGAGCCC
    AGGCTGCTGG TACGACCCAG CACTGAAGAC GTTCCCCAGG CTGGAGTACT ACTCAGCGCC
    GCTCTCAGAG AGGCGGAGAG CCTAAGAGCA GCTCTCTCCT CTCATTGTGC TTCCACTCCT
    CCCGTCCCGT CCGGTCCAGA AGATCTAAAC AAACCTGAAC CCTGCACTCC TCCATCTCCT
    CCTCTGCCTC CTGAAAGTCC ACTGACAGAC CCCATCCAGA GTGAGGAGGA GGAGTGGAGA
    ACTGAGGAAA GCTCAGAAGC ACAGACCTTT AATCACAACT CGGACTCTGA CCACAATCTA
    CAGCTGAAGG TGAGTCAGAG TGTGCAGAGC TTGACTCAGC TGCTGTACAG TCTACAGGCT
    GCAGTTACCA TCCAAGACAG TTGCTATGAA GTGCAGAAGC TTCTTCTCCT GCTGATGTCA
    AGCCCCGCCC CTAACCAACA GTCCCGCCCA CTATCCATCA CCCGCAGCGG GCAGGCTCTG
    CATGAGCAGG AGCGCCAGCG GGAGGCTGAG CGCCGCCGGG AAGAGCTGGC GGATGTGGCT
    CGTCTGCGGG GAGCCCTGGT ACGAGAGCGC CAGCAGTGGG AGAGGGAGTG CCAGGCGCGG
    CAGCTTCAGC ACTCAGAACT GGAGACCACG CTTGAGCAGA GGGAGCTCTT GTGCCACCTA
    GAGGAGCGGA GACTGCAGAG CGAGCGGCAG GAGCTCCAGG AGCAGCTGCA GGAGTACCAA
    CAGAGTCTGG AGAGGCTCAG AGAAGGCCAG AGGAATGTGG AGAAGGAGAA GGAGAAACTG
    GAGACTCAGA AGATGCTGCT GGAGGGCTTG AAGCACGGGT GCCAGCAGAA CCTTCCAGCT
    ATGGTCATAC CACTGGACGG ACAGCAGACA CAGAATCACC TGGGCATTCA GGACGAGCAT
    GGCTCTATTT TTGTCAATGA GGCAGCGTTC ATATCTTCTT CTGTAAATAA CAGGCACCTC
    CATCTTCAGC ATCATCCACA GCATCATCAC CAGACTGTCC ATGTGCACTC AGGATCTATA
    GGATACTCAG ATTCTCCCAG CGCTCAGAAC AGTCTTAATT CACTGCTAGC CCGATCCAAC
    CACAGACCCA GCTCTGCTGG AGCGTCCGAC CACAGCTGGA CGTCTCTGAG GAGTCTGACA
    GAGAGTGCAA ACAACAATGT GGGTCAGATC AGCCATGTTG AAGAGTCTTG GTCAGCAGGG
    GTAACGGGAG CGGGACTCCA TCAAACTGAT GTCCTGCTCG TCAGCCAGCC CATCCTAAGC
    ATGGAGACAG AGAACAGGGA TACTGATGGA GAGGAGACCA TCGTGTATCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144576
    Clone ID Related Accession (Same CDS sequence) XM_009301598.3
    Accession Version XM_009301598.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394590.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301598.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GCCCGAGTCT GGTTGCCTTG
    GAAATGGACA GCGAGGAGGA TGACGATGAT GACATTTTAA TGAAGAAGCC CCTCCCACAA
    CTGCAACCGG ATTCCAAAGA CAGCACAGAT GAGCAGGTTA TGTCCAGCCC CGCACCTGTA
    CTCATCTGCA CGCGCTCTCA CTCTGCCTCC TCTGCCTGTA ACCCCGCCCC TGCAAAGGAT
    GTTGAGCAGG GTGTCCGACT CCGATCCTAT TCCTACACCT CCCCCAAAAT CAGCCTTCTG
    CCTCCACGTT TCAGCCGAGA CGCCCATCCT CCACCCGCCG ACCTCAGCCA GGATGGCGTT
    CTCTCCAGCA GCAGTGGCAG CAAGTCTCTT CTACACGCCC TGTCTCTGTC TAAATCTCTG
    TCTCTGCTCA ACCCTGTTAA GCACCGCTCT TCCAGCACAT CAGAGCAGCC TCAAGAGAAA
    AGGGAGTTAA GGTTTCGCAG GCGAGCTCAG TCGGCTGATG ATGAAAGCAG TGCAGAGCTG
    GCCGACTCGC TCCAACATCT CACGCTCTCC GAGTTCCTCA AAGAGATTGA GGAAGAAGAA
    TGGGATAAAT ATATCATCCC TTCCAAAACA GAGTCAGAGA AGTACAAAGT CAGCCGCACA
    TTCAGCTTCA TAAAGAGCCG AATGTATAGC ACTCGCAACA AGAACAAGGG GAAAGGAAAA
    GAGAAAGAGA CCAAAGACAA GCAGGTCAAC GGACACCAGT TCACAGCGGG ATCTGTCCTG
    GGGTCGACAC TCTGTGAGAT CTGCAGCAAG CCAGCTTCAG GGAAAGAGGT TGTCCATTGC
    ACACACTGTG CAGTGAGTGT CCATAAGGGC TGTAAGGACT CATCTACGCC ATGCCTGAAG
    AAACCTCAAG ACAAATATTC AGTGATGACA GTAAAAAGCA AGACAGCCTC TCTTCCTCAA
    AATTTTATTG TGCGTGAGAG TTCATCGTCG TCCCTTCAGA TCTCTACCTC TGCTTCTCTC
    CCTGTCATGA CATCACGAGA CTCGGCTCCT CTTCCTTGTC CTCTGTCCAG AAGTGTGCCC
    TTAACCACTG AAAGGTTTAG CGAGAGCCCA GATGCTGATA GTGATTCTCC TCTTTGGAGG
    AACCACATCC AATCAGAGGA GCTTCTCCAA ACCATGGAGT CCTCAACATC CACAGACTCG
    TCCATCATTG AAGACTCGGT TGATTCCCCT TTACAGGTTG AGCTGCTGAT GAATGCTGTA
    GATTTGGAGG CAGAGTCATG GAGTTTAGCT GTGGAGGCCC AGTTCTGTAG GATGCAAGAA
    AAACGCATCA TCAAGAGACA GGATGTGATT TACGAGTTCA TGCAGACCGA GCTCCATCAC
    GTGCAGACAC TCATGGTGAT GGCAGAGGTC TTCAGAAGGG GAATGCGGGA TGAAGTGGGT
    CTGGACGCAG AGACCATTGG CCGGATTTTT CCTTGCTTAG ATGAGCTGCT CCAACTCCAT
    CGTGATTTTC TGACAGCAAT GAGGGAACGA AGGCAAAGCT GCGTCCAGAA CAGCAGCAGC
    AAAAACTTTC TCATCCACCG TGTGGGTGAT ATTTTTCTGC AACAGTTCTC CCAGGAGAAC
    GCAGAGAAGA TGAAGCAGGT GTATGGAGAG TTTTGCAGTC ATCACACAGA AGCAGTCAGT
    TACTTCAAAG AGCTTCAGCA GCAGAACAAG AGATTTCAGC TCTTCATCAA ACAACAGAGT
    AGTAACTCAC TGGTGAAGAG GCGAGAGATC CCAGAATGTA TTTTGTTAGT GACGCAGCGA
    ATTACAAAGT ACCCTGTCCT CCTAGAGAGA ATACTCCAGT ACACAGACGA GGGCACAGAA
    GAGCACTCTG ACCTCTCTCG AGCTCTAGTC CTGATCCGAG AGCTGATCTC CGCTGTGGAC
    CAATATGTGA GTCAGTATGA GCAGAACCAG AAGCTGAGTG AAGTCTTGGG CCGCATGGAG
    AACAAGTGCT CTACCAAACT GAAGACCGGC TGCACTTTCC GCAAGCAGGA CATCACCAGC
    GGCAGGAATC TGCTGCACCA GGGCCCGCTG CTCTGGAAGA CAGCCACTGG AAGACTGAAA
    GATGTTCTGG CATTTTTGCT GTCTGATTCA CTGGTGTTCC TTCAGGAGAA AGATCAGAAA
    TACATTTTTG CTACTGTGGA CCAGAAGCCT CCAGTGATCT CTCTGCAGAA ACTAATTGTG
    CGTGAGGTGG CCAATGAAGA GAGAGGCATG TTTCTGATCA GTGCATCTTC AGCCGGGCCT
    GAGATGTACG AGGTGCACGC AGCCTCTAAA GATGAACGCA ACGCCTGGAT GAGACTCATC
    AGAGAGGCCG TGGAGAATTG CCCTGAAGAG GAGGATGAAA CTACAAGTGA GTCAGAAGAG
    GAAAGGAGAG TCACAGAAGC AAAGGTCCAA AAGATCCACC GGCTGCAAGA GACGCTGTGT
    GGCCATGATC ACCTCATCTG TTCCAATCTG GAGGAGAAGC TGCACATCTA TGCCGAGCTG
    TCAGTGATGG CAGGCCAGCG AGAGGCAGGA CCAGAGCCCA GGCTGCTGGT ACGACCCAGC
    ACTGAAGACG TTCCCCAGGC TGGAGTACTA CTCAGCGCCG CTCTCAGAGA GGCGGAGAGC
    CTAAGAGCAG CTCTCTCCTC TCATTGTGCT TCCACTCCTC CCGTCCCGTC CGGTCCAGAA
    GATCTAAACA AACCTGAACC CTGCACTCCT CCATCTCCTC CTCTGCCTCC TGAAAGTCCA
    CTGACAGACC CCATCCAGAG TGAGGAGGAG GAGTGGAGAA CTGAGGAAAG CTCAGAAGCA
    CAGACCTTTA ATCACAACTC GGACTCTGAC CACAATCTAC AGCTGAAGGT GAGTCAGAGT
    GTGCAGAGCT TGACTCAGCT GCTGTACAGT CTACAGGCTG CAGTTACCAT CCAAGACAGT
    TGCTATGAAG TGCAGAAGCT TCTTCTCCTG CTGATGTCAA GCCCCGCCCC TAACCAACAG
    TCCCGCCCAC TATCCATCAC CCGCAGCGGG CAGGCTCTGC ATGAGCAGGA GCGCCAGCGG
    GAGGCTGAGC GCCGCCGGGA AGAGCTGGCG GATGTGGCTC GTCTGCGGGG AGCCCTGGTA
    CGAGAGCGCC AGCAGTGGGA GAGGGAGTGC CAGGCGCGGC AGCTTCAGCA CTCAGAACTG
    GAGACCACGC TTGAGCAGAG GGAGCTCTTG TGCCACCTAG AGGAGCGGAG ACTGCAGAGC
    GAGCGGCAGG AGCTCCAGGA GCAGCTGCAG GAGTACCAAC AGAGTCTGGA GAGGCTCAGA
    GAAGGCCAGA GGAATGTGGA GAAGGAGAAG GAGAAACTGG AGACTCAGAA GATGCTGCTG
    GAGGGCTTGA AGCACGGGTG CCAGCAGAAC CTTCCAGCTA TGGTCATACC ACTGGACGGA
    CAGCAGACAC AGAATCACCT GGGCATTCAG GACGAGCATG GCTCTATTTT TGTCAATGAG
    GCAGCGTTCA TATCTTCTTC TGTAAATAAC AGGCACCTCC ATCTTCAGCA TCATCCACAG
    CATCATCACC AGACTGTCCA TGTGCACTCA GGATCTATAG GATACTCAGA TTCTCCCAGC
    GCTCAGAACA GTCTTAATTC ACTGCTAGCC CGATCCAACC ACAGACCCAG CTCTGCTGGA
    GCGTCCGACC ACAGCTGGAC GTCTCTGAGG AGTCTGACAG AGAGTGCAAA CAACAATGTG
    GGTCAGATCA GCCATGTTGA AGAGTCTTGG TCAGCAGGGG TAACGGGAGC GGGACTCCAT
    CAAACTGATG TCCTGCTCGT CAGCCAGCCC ATCCTAAGCA TGGAGACAGA GAACAGGGAT
    ACTGATGGAG AGGAGACCAT CGTGTATCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299873.2
    CDS207..5639
    Translation

    Target ORF information:

    RefSeq Version XM_009301598.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301598.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GCCCGAGTCT GGTTGCCTTG
    GAAATGGACA GCGAGGAGGA TGACGATGAT GACATTTTAA TGAAGAAGCC CCTCCCACAA
    CTGCAACCGG ATTCCAAAGA CAGCACAGAT GAGCAGGTTA TGTCCAGCCC CGCACCTGTA
    CTCATCTGCA CGCGCTCTCA CTCTGCCTCC TCTGCCTGTA ACCCCGCCCC TGCAAAGGAT
    GTTGAGCAGG GTGTCCGACT CCGATCCTAT TCCTACACCT CCCCCAAAAT CAGCCTTCTG
    CCTCCACGTT TCAGCCGAGA CGCCCATCCT CCACCCGCCG ACCTCAGCCA GGATGGCGTT
    CTCTCCAGCA GCAGTGGCAG CAAGTCTCTT CTACACGCCC TGTCTCTGTC TAAATCTCTG
    TCTCTGCTCA ACCCTGTTAA GCACCGCTCT TCCAGCACAT CAGAGCAGCC TCAAGAGAAA
    AGGGAGTTAA GGTTTCGCAG GCGAGCTCAG TCGGCTGATG ATGAAAGCAG TGCAGAGCTG
    GCCGACTCGC TCCAACATCT CACGCTCTCC GAGTTCCTCA AAGAGATTGA GGAAGAAGAA
    TGGGATAAAT ATATCATCCC TTCCAAAACA GAGTCAGAGA AGTACAAAGT CAGCCGCACA
    TTCAGCTTCA TAAAGAGCCG AATGTATAGC ACTCGCAACA AGAACAAGGG GAAAGGAAAA
    GAGAAAGAGA CCAAAGACAA GCAGGTCAAC GGACACCAGT TCACAGCGGG ATCTGTCCTG
    GGGTCGACAC TCTGTGAGAT CTGCAGCAAG CCAGCTTCAG GGAAAGAGGT TGTCCATTGC
    ACACACTGTG CAGTGAGTGT CCATAAGGGC TGTAAGGACT CATCTACGCC ATGCCTGAAG
    AAACCTCAAG ACAAATATTC AGTGATGACA GTAAAAAGCA AGACAGCCTC TCTTCCTCAA
    AATTTTATTG TGCGTGAGAG TTCATCGTCG TCCCTTCAGA TCTCTACCTC TGCTTCTCTC
    CCTGTCATGA CATCACGAGA CTCGGCTCCT CTTCCTTGTC CTCTGTCCAG AAGTGTGCCC
    TTAACCACTG AAAGGTTTAG CGAGAGCCCA GATGCTGATA GTGATTCTCC TCTTTGGAGG
    AACCACATCC AATCAGAGGA GCTTCTCCAA ACCATGGAGT CCTCAACATC CACAGACTCG
    TCCATCATTG AAGACTCGGT TGATTCCCCT TTACAGGTTG AGCTGCTGAT GAATGCTGTA
    GATTTGGAGG CAGAGTCATG GAGTTTAGCT GTGGAGGCCC AGTTCTGTAG GATGCAAGAA
    AAACGCATCA TCAAGAGACA GGATGTGATT TACGAGTTCA TGCAGACCGA GCTCCATCAC
    GTGCAGACAC TCATGGTGAT GGCAGAGGTC TTCAGAAGGG GAATGCGGGA TGAAGTGGGT
    CTGGACGCAG AGACCATTGG CCGGATTTTT CCTTGCTTAG ATGAGCTGCT CCAACTCCAT
    CGTGATTTTC TGACAGCAAT GAGGGAACGA AGGCAAAGCT GCGTCCAGAA CAGCAGCAGC
    AAAAACTTTC TCATCCACCG TGTGGGTGAT ATTTTTCTGC AACAGTTCTC CCAGGAGAAC
    GCAGAGAAGA TGAAGCAGGT GTATGGAGAG TTTTGCAGTC ATCACACAGA AGCAGTCAGT
    TACTTCAAAG AGCTTCAGCA GCAGAACAAG AGATTTCAGC TCTTCATCAA ACAACAGAGT
    AGTAACTCAC TGGTGAAGAG GCGAGAGATC CCAGAATGTA TTTTGTTAGT GACGCAGCGA
    ATTACAAAGT ACCCTGTCCT CCTAGAGAGA ATACTCCAGT ACACAGACGA GGGCACAGAA
    GAGCACTCTG ACCTCTCTCG AGCTCTAGTC CTGATCCGAG AGCTGATCTC CGCTGTGGAC
    CAATATGTGA GTCAGTATGA GCAGAACCAG AAGCTGAGTG AAGTCTTGGG CCGCATGGAG
    AACAAGTGCT CTACCAAACT GAAGACCGGC TGCACTTTCC GCAAGCAGGA CATCACCAGC
    GGCAGGAATC TGCTGCACCA GGGCCCGCTG CTCTGGAAGA CAGCCACTGG AAGACTGAAA
    GATGTTCTGG CATTTTTGCT GTCTGATTCA CTGGTGTTCC TTCAGGAGAA AGATCAGAAA
    TACATTTTTG CTACTGTGGA CCAGAAGCCT CCAGTGATCT CTCTGCAGAA ACTAATTGTG
    CGTGAGGTGG CCAATGAAGA GAGAGGCATG TTTCTGATCA GTGCATCTTC AGCCGGGCCT
    GAGATGTACG AGGTGCACGC AGCCTCTAAA GATGAACGCA ACGCCTGGAT GAGACTCATC
    AGAGAGGCCG TGGAGAATTG CCCTGAAGAG GAGGATGAAA CTACAAGTGA GTCAGAAGAG
    GAAAGGAGAG TCACAGAAGC AAAGGTCCAA AAGATCCACC GGCTGCAAGA GACGCTGTGT
    GGCCATGATC ACCTCATCTG TTCCAATCTG GAGGAGAAGC TGCACATCTA TGCCGAGCTG
    TCAGTGATGG CAGGCCAGCG AGAGGCAGGA CCAGAGCCCA GGCTGCTGGT ACGACCCAGC
    ACTGAAGACG TTCCCCAGGC TGGAGTACTA CTCAGCGCCG CTCTCAGAGA GGCGGAGAGC
    CTAAGAGCAG CTCTCTCCTC TCATTGTGCT TCCACTCCTC CCGTCCCGTC CGGTCCAGAA
    GATCTAAACA AACCTGAACC CTGCACTCCT CCATCTCCTC CTCTGCCTCC TGAAAGTCCA
    CTGACAGACC CCATCCAGAG TGAGGAGGAG GAGTGGAGAA CTGAGGAAAG CTCAGAAGCA
    CAGACCTTTA ATCACAACTC GGACTCTGAC CACAATCTAC AGCTGAAGGT GAGTCAGAGT
    GTGCAGAGCT TGACTCAGCT GCTGTACAGT CTACAGGCTG CAGTTACCAT CCAAGACAGT
    TGCTATGAAG TGCAGAAGCT TCTTCTCCTG CTGATGTCAA GCCCCGCCCC TAACCAACAG
    TCCCGCCCAC TATCCATCAC CCGCAGCGGG CAGGCTCTGC ATGAGCAGGA GCGCCAGCGG
    GAGGCTGAGC GCCGCCGGGA AGAGCTGGCG GATGTGGCTC GTCTGCGGGG AGCCCTGGTA
    CGAGAGCGCC AGCAGTGGGA GAGGGAGTGC CAGGCGCGGC AGCTTCAGCA CTCAGAACTG
    GAGACCACGC TTGAGCAGAG GGAGCTCTTG TGCCACCTAG AGGAGCGGAG ACTGCAGAGC
    GAGCGGCAGG AGCTCCAGGA GCAGCTGCAG GAGTACCAAC AGAGTCTGGA GAGGCTCAGA
    GAAGGCCAGA GGAATGTGGA GAAGGAGAAG GAGAAACTGG AGACTCAGAA GATGCTGCTG
    GAGGGCTTGA AGCACGGGTG CCAGCAGAAC CTTCCAGCTA TGGTCATACC ACTGGACGGA
    CAGCAGACAC AGAATCACCT GGGCATTCAG GACGAGCATG GCTCTATTTT TGTCAATGAG
    GCAGCGTTCA TATCTTCTTC TGTAAATAAC AGGCACCTCC ATCTTCAGCA TCATCCACAG
    CATCATCACC AGACTGTCCA TGTGCACTCA GGATCTATAG GATACTCAGA TTCTCCCAGC
    GCTCAGAACA GTCTTAATTC ACTGCTAGCC CGATCCAACC ACAGACCCAG CTCTGCTGGA
    GCGTCCGACC ACAGCTGGAC GTCTCTGAGG AGTCTGACAG AGAGTGCAAA CAACAATGTG
    GGTCAGATCA GCCATGTTGA AGAGTCTTGG TCAGCAGGGG TAACGGGAGC GGGACTCCAT
    CAAACTGATG TCCTGCTCGT CAGCCAGCCC ATCCTAAGCA TGGAGACAGA GAACAGGGAT
    ACTGATGGAG AGGAGACCAT CGTGTATCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144577
    Clone ID Related Accession (Same CDS sequence) XM_017356270.2
    Accession Version XM_017356270.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394590.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAGC AGCCTCAAGA GAAAAGGGAG
    TTAAGGTTTC GCAGGCGAGC TCAGTCGGCT GATGATGAAA GCAGTGCAGA GCTGGCCGAC
    TCGCTCCAAC ATCTCACGCT CTCCGAGTTC CTCAAAGAGA TTGAGGAAGA AGAATGGGAT
    AAATATATCA TCCCTTCCAA AACAGAGTCA GAGAAGTACA AAGTCAGCCG CACATTCAGC
    TTCATAAAGA GCCGAATGTA TAGCACTCGC AACAAGAACA AGGGGAAAGG AAAAGAGAAA
    GAGACCAAAG ACAAGCAGGT CAACGGACAC CAGTTCACAG CGGGATCTGT CCTGGGGTCG
    ACACTCTGTG AGATCTGCAG CAAGCCAGCT TCAGGGAAAG AGGTTGTCCA TTGCACACAC
    TGTGCAGTGA GTGTCCATAA GGGCTGTAAG GACTCATCTA CGCCATGCCT GAAGAAACCT
    CAAGACAAAT ATTCAGTGAT GACAGTAAAA AGCAAGACAG CCTCTCTTCC TCAAAATTTT
    ATTGTGCGTG AGAGTTCATC GTCGTCCCTT CAGATCTCTA CCTCTGCTTC TCTCCCTGTC
    ATGACATCAC GAGACTCGGC TCCTCTTCCT TGTCCTCTGT CCAGAAGTGT GCCCTTAACC
    ACTGAAAGGT TTAGCGAGAG CCCAGATGCT GATAGTGATT CTCCTCTTTG GAGGAACCAC
    ATCCAATCAG AGGAGCTTCT CCAAACCATG GAGTCCTCAA CATCCACAGA CTCGTCCATC
    ATTGAAGACT CGGTTGATTC CCCTTTACAG GTTGAGCTGC TGATGAATGC TGTAGATTTG
    GAGGCAGAGT CATGGAGTTT AGCTGTGGAG GCCCAGTTCT GTAGGATGCA AGAAAAACGC
    ATCATCAAGA GACAGGATGT GATTTACGAG TTCATGCAGA CCGAGCTCCA TCACGTGCAG
    ACACTCATGG TGATGGCAGA GGTCTTCAGA AGGGGAATGC GGGATGAAGT GGGTCTGGAC
    GCAGAGACCA TTGGCCGGAT TTTTCCTTGC TTAGATGAGC TGCTCCAACT CCATCGTGAT
    TTTCTGACAG CAATGAGGGA ACGAAGGCAA AGCTGCGTCC AGAACAGCAG CAGCAAAAAC
    TTTCTCATCC ACCGTGTGGG TGATATTTTT CTGCAACAGT TCTCCCAGGA GAACGCAGAG
    AAGATGAAGC AGGTGTATGG AGAGTTTTGC AGTCATCACA CAGAAGCAGT CAGTTACTTC
    AAAGAGCTTC AGCAGCAGAA CAAGAGATTT CAGCTCTTCA TCAAACAACA GAGTAGTAAC
    TCACTGGTGA AGAGGCGAGA GATCCCAGAA TGTATTTTGT TAGTGACGCA GCGAATTACA
    AAGTACCCTG TCCTCCTAGA GAGAATACTC CAGTACACAG ACGAGGGCAC AGAAGAGCAC
    TCTGACCTCT CTCGAGCTCT AGTCCTGATC CGAGAGCTGA TCTCCGCTGT GGACCAATAT
    GTGAGTCAGT ATGAGCAGAA CCAGAAGCTG AGTGAAGTCT TGGGCCGCAT GGAGAACAAG
    TGCTCTACCA AACTGAAGAC CGGCTGCACT TTCCGCAAGC AGGACATCAC CAGCGGCAGG
    AATCTGCTGC ACCAGGGCCC GCTGCTCTGG AAGACAGCCA CTGGAAGACT GAAAGATGTT
    CTGGCATTTT TGCTGTCTGA TTCACTGGTG TTCCTTCAGG AGAAAGATCA GAAATACATT
    TTTGCTACTG TGGACCAGAA GCCTCCAGTG ATCTCTCTGC AGAAACTAAT TGTGCGTGAG
    GTGGCCAATG AAGAGAGAGG CATGTTTCTG ATCAGTGCAT CTTCAGCCGG GCCTGAGATG
    TACGAGGTGC ACGCAGCCTC TAAAGATGAA CGCAACGCCT GGATGAGACT CATCAGAGAG
    GCCGTGGAGA ATTGCCCTGA AGAGGAGGAT GAAACTACAA GTGAGTCAGA AGAGGAAAGG
    AGAGTCACAG AAGCAAAGGT CCAAAAGATC CACCGGCTGC AAGAGACGCT GTGTGGCCAT
    GATCACCTCA TCTGTTCCAA TCTGGAGGAG AAGCTGCACA TCTATGCCGA GCTGTCAGTG
    ATGGCAGGCC AGCGAGAGGC AGGACCAGAG CCCAGGCTGC TGGTACGACC CAGCACTGAA
    GACGTTCCCC AGGCTGGAGT ACTACTCAGC GCCGCTCTCA GAGAGGCGGA GAGCCTAAGA
    GCAGCTCTCT CCTCTCATTG TGCTTCCACT CCTCCCGTCC CGTCCGGTCC AGAAGATCTA
    AACAAACCTG AACCCTGCAC TCCTCCATCT CCTCCTCTGC CTCCTGAAAG TCCACTGACA
    GACCCCATCC AGAGTGAGGA GGAGGAGTGG AGAACTGAGG AAAGCTCAGA AGCACAGACC
    TTTAATCACA ACTCGGACTC TGACCACAAT CTACAGCTGA AGGTGAGTCA GAGTGTGCAG
    AGCTTGACTC AGCTGCTGTA CAGTCTACAG GCTGCAGTTA CCATCCAAGA CAGTTGCTAT
    GAAGTGCAGA AGCTTCTTCT CCTGCTGATG TCAAGCCCCG CCCCTAACCA ACAGTCCCGC
    CCACTATCCA TCACCCGCAG CGGGCAGGCT CTGCATGAGC AGGAGCGCCA GCGGGAGGCT
    GAGCGCCGCC GGGAAGAGCT GGCGGATGTG GCTCGTCTGC GGGGAGCCCT GGTACGAGAG
    CGCCAGCAGT GGGAGAGGGA GTGCCAGGCG CGGCAGCTTC AGCACTCAGA ACTGGAGACC
    ACGCTTGAGC AGAGGGAGCT CTTGTGCCAC CTAGAGGAGC GGAGACTGCA GAGCGAGCGG
    CAGGAGCTCC AGGAGCAGCT GCAGGAGTAC CAACAGAGTC TGGAGAGGCT CAGAGAAGGC
    CAGAGGAATG TGGAGAAGGA GAAGGAGAAA CTGGAGACTC AGAAGATGCT GCTGGAGGGC
    TTGAAGCACG GGTGCCAGCA GAACCTTCCA GCTATGGTCA TACCACTGGA CGGACAGCAG
    ACACAGAATC ACCTGGGCAT TCAGGACGAG CATGGCTCTA TTTTTGTCAA TGAGGCAGCG
    TTCATATCTT CTTCTGTAAA TAACAGGCAC CTCCATCTTC AGCATCATCC ACAGCATCAT
    CACCAGACTG TCCATGTGCA CTCAGGATCT ATAGGATACT CAGATTCTCC CAGCGCTCAG
    AACAGTCTTA ATTCACTGCT AGCCCGATCC AACCACAGAC CCAGCTCTGC TGGAGCGTCC
    GACCACAGCT GGACGTCTCT GAGGAGTCTG ACAGAGAGTG CAAACAACAA TGTGGGTCAG
    ATCAGCCATG TTGAAGAGTC TTGGTCAGCA GGGGTAACGG GAGCGGGACT CCATCAAACT
    GATGTCCTGC TCGTCAGCCA GCCCATCCTA AGCATGGAGA CAGAGAACAG GGATACTGAT
    GGAGAGGAGA CCATCGTGTA TCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211759.2
    CDS207..5573
    Translation

    Target ORF information:

    RefSeq Version XM_017356270.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356270.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAGC AGCCTCAAGA GAAAAGGGAG
    TTAAGGTTTC GCAGGCGAGC TCAGTCGGCT GATGATGAAA GCAGTGCAGA GCTGGCCGAC
    TCGCTCCAAC ATCTCACGCT CTCCGAGTTC CTCAAAGAGA TTGAGGAAGA AGAATGGGAT
    AAATATATCA TCCCTTCCAA AACAGAGTCA GAGAAGTACA AAGTCAGCCG CACATTCAGC
    TTCATAAAGA GCCGAATGTA TAGCACTCGC AACAAGAACA AGGGGAAAGG AAAAGAGAAA
    GAGACCAAAG ACAAGCAGGT CAACGGACAC CAGTTCACAG CGGGATCTGT CCTGGGGTCG
    ACACTCTGTG AGATCTGCAG CAAGCCAGCT TCAGGGAAAG AGGTTGTCCA TTGCACACAC
    TGTGCAGTGA GTGTCCATAA GGGCTGTAAG GACTCATCTA CGCCATGCCT GAAGAAACCT
    CAAGACAAAT ATTCAGTGAT GACAGTAAAA AGCAAGACAG CCTCTCTTCC TCAAAATTTT
    ATTGTGCGTG AGAGTTCATC GTCGTCCCTT CAGATCTCTA CCTCTGCTTC TCTCCCTGTC
    ATGACATCAC GAGACTCGGC TCCTCTTCCT TGTCCTCTGT CCAGAAGTGT GCCCTTAACC
    ACTGAAAGGT TTAGCGAGAG CCCAGATGCT GATAGTGATT CTCCTCTTTG GAGGAACCAC
    ATCCAATCAG AGGAGCTTCT CCAAACCATG GAGTCCTCAA CATCCACAGA CTCGTCCATC
    ATTGAAGACT CGGTTGATTC CCCTTTACAG GTTGAGCTGC TGATGAATGC TGTAGATTTG
    GAGGCAGAGT CATGGAGTTT AGCTGTGGAG GCCCAGTTCT GTAGGATGCA AGAAAAACGC
    ATCATCAAGA GACAGGATGT GATTTACGAG TTCATGCAGA CCGAGCTCCA TCACGTGCAG
    ACACTCATGG TGATGGCAGA GGTCTTCAGA AGGGGAATGC GGGATGAAGT GGGTCTGGAC
    GCAGAGACCA TTGGCCGGAT TTTTCCTTGC TTAGATGAGC TGCTCCAACT CCATCGTGAT
    TTTCTGACAG CAATGAGGGA ACGAAGGCAA AGCTGCGTCC AGAACAGCAG CAGCAAAAAC
    TTTCTCATCC ACCGTGTGGG TGATATTTTT CTGCAACAGT TCTCCCAGGA GAACGCAGAG
    AAGATGAAGC AGGTGTATGG AGAGTTTTGC AGTCATCACA CAGAAGCAGT CAGTTACTTC
    AAAGAGCTTC AGCAGCAGAA CAAGAGATTT CAGCTCTTCA TCAAACAACA GAGTAGTAAC
    TCACTGGTGA AGAGGCGAGA GATCCCAGAA TGTATTTTGT TAGTGACGCA GCGAATTACA
    AAGTACCCTG TCCTCCTAGA GAGAATACTC CAGTACACAG ACGAGGGCAC AGAAGAGCAC
    TCTGACCTCT CTCGAGCTCT AGTCCTGATC CGAGAGCTGA TCTCCGCTGT GGACCAATAT
    GTGAGTCAGT ATGAGCAGAA CCAGAAGCTG AGTGAAGTCT TGGGCCGCAT GGAGAACAAG
    TGCTCTACCA AACTGAAGAC CGGCTGCACT TTCCGCAAGC AGGACATCAC CAGCGGCAGG
    AATCTGCTGC ACCAGGGCCC GCTGCTCTGG AAGACAGCCA CTGGAAGACT GAAAGATGTT
    CTGGCATTTT TGCTGTCTGA TTCACTGGTG TTCCTTCAGG AGAAAGATCA GAAATACATT
    TTTGCTACTG TGGACCAGAA GCCTCCAGTG ATCTCTCTGC AGAAACTAAT TGTGCGTGAG
    GTGGCCAATG AAGAGAGAGG CATGTTTCTG ATCAGTGCAT CTTCAGCCGG GCCTGAGATG
    TACGAGGTGC ACGCAGCCTC TAAAGATGAA CGCAACGCCT GGATGAGACT CATCAGAGAG
    GCCGTGGAGA ATTGCCCTGA AGAGGAGGAT GAAACTACAA GTGAGTCAGA AGAGGAAAGG
    AGAGTCACAG AAGCAAAGGT CCAAAAGATC CACCGGCTGC AAGAGACGCT GTGTGGCCAT
    GATCACCTCA TCTGTTCCAA TCTGGAGGAG AAGCTGCACA TCTATGCCGA GCTGTCAGTG
    ATGGCAGGCC AGCGAGAGGC AGGACCAGAG CCCAGGCTGC TGGTACGACC CAGCACTGAA
    GACGTTCCCC AGGCTGGAGT ACTACTCAGC GCCGCTCTCA GAGAGGCGGA GAGCCTAAGA
    GCAGCTCTCT CCTCTCATTG TGCTTCCACT CCTCCCGTCC CGTCCGGTCC AGAAGATCTA
    AACAAACCTG AACCCTGCAC TCCTCCATCT CCTCCTCTGC CTCCTGAAAG TCCACTGACA
    GACCCCATCC AGAGTGAGGA GGAGGAGTGG AGAACTGAGG AAAGCTCAGA AGCACAGACC
    TTTAATCACA ACTCGGACTC TGACCACAAT CTACAGCTGA AGGTGAGTCA GAGTGTGCAG
    AGCTTGACTC AGCTGCTGTA CAGTCTACAG GCTGCAGTTA CCATCCAAGA CAGTTGCTAT
    GAAGTGCAGA AGCTTCTTCT CCTGCTGATG TCAAGCCCCG CCCCTAACCA ACAGTCCCGC
    CCACTATCCA TCACCCGCAG CGGGCAGGCT CTGCATGAGC AGGAGCGCCA GCGGGAGGCT
    GAGCGCCGCC GGGAAGAGCT GGCGGATGTG GCTCGTCTGC GGGGAGCCCT GGTACGAGAG
    CGCCAGCAGT GGGAGAGGGA GTGCCAGGCG CGGCAGCTTC AGCACTCAGA ACTGGAGACC
    ACGCTTGAGC AGAGGGAGCT CTTGTGCCAC CTAGAGGAGC GGAGACTGCA GAGCGAGCGG
    CAGGAGCTCC AGGAGCAGCT GCAGGAGTAC CAACAGAGTC TGGAGAGGCT CAGAGAAGGC
    CAGAGGAATG TGGAGAAGGA GAAGGAGAAA CTGGAGACTC AGAAGATGCT GCTGGAGGGC
    TTGAAGCACG GGTGCCAGCA GAACCTTCCA GCTATGGTCA TACCACTGGA CGGACAGCAG
    ACACAGAATC ACCTGGGCAT TCAGGACGAG CATGGCTCTA TTTTTGTCAA TGAGGCAGCG
    TTCATATCTT CTTCTGTAAA TAACAGGCAC CTCCATCTTC AGCATCATCC ACAGCATCAT
    CACCAGACTG TCCATGTGCA CTCAGGATCT ATAGGATACT CAGATTCTCC CAGCGCTCAG
    AACAGTCTTA ATTCACTGCT AGCCCGATCC AACCACAGAC CCAGCTCTGC TGGAGCGTCC
    GACCACAGCT GGACGTCTCT GAGGAGTCTG ACAGAGAGTG CAAACAACAA TGTGGGTCAG
    ATCAGCCATG TTGAAGAGTC TTGGTCAGCA GGGGTAACGG GAGCGGGACT CCATCAAACT
    GATGTCCTGC TCGTCAGCCA GCCCATCCTA AGCATGGAGA CAGAGAACAG GGATACTGAT
    GGAGAGGAGA CCATCGTGTA TCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144578
    Clone ID Related Accession (Same CDS sequence) XM_017356271.2
    Accession Version XM_017356271.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394590.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGCTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAGCAGC CTCAAGAGAA AAGGATTGAG GAAGAAGAAT GGGATAAATA TATCATCCCT
    TCCAAAACAG AGTCAGAGAA GTACAAAGTC AGCCGCACAT TCAGCTTCAT AAAGAGCCGA
    ATGTATAGCA CTCGCAACAA GAACAAGGGG AAAGGAAAAG AGAAAGAGAC CAAAGACAAG
    CAGGTCAACG GACACCAGTT CACAGCGGGA TCTGTCCTGG GGTCGACACT CTGTGAGATC
    TGCAGCAAGC CAGCTTCAGG GAAAGAGGTT GTCCATTGCA CACACTGTGC AGTGAGTGTC
    CATAAGGGCT GTAAGGACTC ATCTACGCCA TGCCTGAAGA AACCTCAAGA CAAATATTCA
    GTGATGACAG TAAAAAGCAA GACAGCCTCT CTTCCTCAAA ATTTTATTGT GCGTGAGAGT
    TCATCGTCGT CCCTTCAGAT CTCTACCTCT GCTTCTCTCC CTGTCATGAC ATCACGAGAC
    TCGGCTCCTC TTCCTTGTCC TCTGTCCAGA AGTGTGCCCT TAACCACTGA AAGGTTTAGC
    GAGAGCCCAG ATGCTGATAG TGATTCTCCT CTTTGGAGGA ACCACATCCA ATCAGAGGAG
    CTTCTCCAAA CCATGGAGTC CTCAACATCC ACAGACTCGT CCATCATTGA AGACTCGGTT
    GATTCCCCTT TACAGGTTGA GCTGCTGATG AATGCTGTAG ATTTGGAGGC AGAGTCATGG
    AGTTTAGCTG TGGAGGCCCA GTTCTGTAGG ATGCAAGAAA AACGCATCAT CAAGAGACAG
    GATGTGATTT ACGAGTTCAT GCAGACCGAG CTCCATCACG TGCAGACACT CATGGTGATG
    GCAGAGGTCT TCAGAAGGGG AATGCGGGAT GAAGTGGGTC TGGACGCAGA GACCATTGGC
    CGGATTTTTC CTTGCTTAGA TGAGCTGCTC CAACTCCATC GTGATTTTCT GACAGCAATG
    AGGGAACGAA GGCAAAGCTG CGTCCAGAAC AGCAGCAGCA AAAACTTTCT CATCCACCGT
    GTGGGTGATA TTTTTCTGCA ACAGTTCTCC CAGGAGAACG CAGAGAAGAT GAAGCAGGTG
    TATGGAGAGT TTTGCAGTCA TCACACAGAA GCAGTCAGTT ACTTCAAAGA GCTTCAGCAG
    CAGAACAAGA GATTTCAGCT CTTCATCAAA CAACAGAGTA GTAACTCACT GGTGAAGAGG
    CGAGAGATCC CAGAATGTAT TTTGTTAGTG ACGCAGCGAA TTACAAAGTA CCCTGTCCTC
    CTAGAGAGAA TACTCCAGTA CACAGACGAG GGCACAGAAG AGCACTCTGA CCTCTCTCGA
    GCTCTAGTCC TGATCCGAGA GCTGATCTCC GCTGTGGACC AATATGTGAG TCAGTATGAG
    CAGAACCAGA AGCTGAGTGA AGTCTTGGGC CGCATGGAGA ACAAGTGCTC TACCAAACTG
    AAGACCGGCT GCACTTTCCG CAAGCAGGAC ATCACCAGCG GCAGGAATCT GCTGCACCAG
    GGCCCGCTGC TCTGGAAGAC AGCCACTGGA AGACTGAAAG ATGTTCTGGC ATTTTTGCTG
    TCTGATTCAC TGGTGTTCCT TCAGGAGAAA GATCAGAAAT ACATTTTTGC TACTGTGGAC
    CAGAAGCCTC CAGTGATCTC TCTGCAGAAA CTAATTGTGC GTGAGGTGGC CAATGAAGAG
    AGAGGCATGT TTCTGATCAG TGCATCTTCA GCCGGGCCTG AGATGTACGA GGTGCACGCA
    GCCTCTAAAG ATGAACGCAA CGCCTGGATG AGACTCATCA GAGAGGCCGT GGAGAATTGC
    CCTGAAGAGG AGGATGAAAC TACAAGTGAG TCAGAAGAGG AAAGGAGAGT CACAGAAGCA
    AAGGTCCAAA AGATCCACCG GCTGCAAGAG ACGCTGTGTG GCCATGATCA CCTCATCTGT
    TCCAATCTGG AGGAGAAGCT GCACATCTAT GCCGAGCTGT CAGTGATGGC AGGCCAGCGA
    GAGGCAGGAC CAGAGCCCAG GCTGCTGGTA CGACCCAGCA CTGAAGACGT TCCCCAGGCT
    GGAGTACTAC TCAGCGCCGC TCTCAGAGAG GCGGAGAGCC TAAGAGCAGC TCTCTCCTCT
    CATTGTGCTT CCACTCCTCC CGTCCCGTCC GGTCCAGAAG ATCTAAACAA ACCTGAACCC
    TGCACTCCTC CATCTCCTCC TCTGCCTCCT GAAAGTCCAC TGACAGACCC CATCCAGAGT
    GAGGAGGAGG AGTGGAGAAC TGAGGAAAGC TCAGAAGCAC AGACCTTTAA TCACAACTCG
    GACTCTGACC ACAATCTACA GCTGAAGGTG AGTCAGAGTG TGCAGAGCTT GACTCAGCTG
    CTGTACAGTC TACAGGCTGC AGTTACCATC CAAGACAGTT GCTATGAAGT GCAGAAGCTT
    CTTCTCCTGC TGATGTCAAG CCCCGCCCCT AACCAACAGT CCCGCCCACT ATCCATCACC
    CGCAGCGGGC AGGCTCTGCA TGAGCAGGAG CGCCAGCGGG AGGCTGAGCG CCGCCGGGAA
    GAGCTGGCGG ATGTGGCTCG TCTGCGGGGA GCCCTGGTAC GAGAGCGCCA GCAGTGGGAG
    AGGGAGTGCC AGGCGCGGCA GCTTCAGCAC TCAGAACTGG AGACCACGCT TGAGCAGAGG
    GAGCTCTTGT GCCACCTAGA GGAGCGGAGA CTGCAGAGCG AGCGGCAGGA GCTCCAGGAG
    CAGCTGCAGG AGTACCAACA GAGTCTGGAG AGGCTCAGAG AAGGCCAGAG GAATGTGGAG
    AAGGAGAAGG AGAAACTGGA GACTCAGAAG ATGCTGCTGG AGGGCTTGAA GCACGGGTGC
    CAGCAGAACC TTCCAGCTAT GGTCATACCA CTGGACGGAC AGCAGACACA GAATCACCTG
    GGCATTCAGG ACGAGCATGG CTCTATTTTT GTCAATGAGG CAGCGTTCAT ATCTTCTTCT
    GTAAATAACA GGCACCTCCA TCTTCAGCAT CATCCACAGC ATCATCACCA GACTGTCCAT
    GTGCACTCAG GATCTATAGG ATACTCAGAT TCTCCCAGCG CTCAGAACAG TCTTAATTCA
    CTGCTAGCCC GATCCAACCA CAGACCCAGC TCTGCTGGAG CGTCCGACCA CAGCTGGACG
    TCTCTGAGGA GTCTGACAGA GAGTGCAAAC AACAATGTGG GTCAGATCAG CCATGTTGAA
    GAGTCTTGGT CAGCAGGGGT AACGGGAGCG GGACTCCATC AAACTGATGT CCTGCTCGTC
    AGCCAGCCCA TCCTAAGCAT GGAGACAGAG AACAGGGATA CTGATGGAGA GGAGACCATC
    GTGTATCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211760.2
    CDS207..5558
    Translation

    Target ORF information:

    RefSeq Version XM_017356271.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356271.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGCTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAGCAGC CTCAAGAGAA AAGGATTGAG GAAGAAGAAT GGGATAAATA TATCATCCCT
    TCCAAAACAG AGTCAGAGAA GTACAAAGTC AGCCGCACAT TCAGCTTCAT AAAGAGCCGA
    ATGTATAGCA CTCGCAACAA GAACAAGGGG AAAGGAAAAG AGAAAGAGAC CAAAGACAAG
    CAGGTCAACG GACACCAGTT CACAGCGGGA TCTGTCCTGG GGTCGACACT CTGTGAGATC
    TGCAGCAAGC CAGCTTCAGG GAAAGAGGTT GTCCATTGCA CACACTGTGC AGTGAGTGTC
    CATAAGGGCT GTAAGGACTC ATCTACGCCA TGCCTGAAGA AACCTCAAGA CAAATATTCA
    GTGATGACAG TAAAAAGCAA GACAGCCTCT CTTCCTCAAA ATTTTATTGT GCGTGAGAGT
    TCATCGTCGT CCCTTCAGAT CTCTACCTCT GCTTCTCTCC CTGTCATGAC ATCACGAGAC
    TCGGCTCCTC TTCCTTGTCC TCTGTCCAGA AGTGTGCCCT TAACCACTGA AAGGTTTAGC
    GAGAGCCCAG ATGCTGATAG TGATTCTCCT CTTTGGAGGA ACCACATCCA ATCAGAGGAG
    CTTCTCCAAA CCATGGAGTC CTCAACATCC ACAGACTCGT CCATCATTGA AGACTCGGTT
    GATTCCCCTT TACAGGTTGA GCTGCTGATG AATGCTGTAG ATTTGGAGGC AGAGTCATGG
    AGTTTAGCTG TGGAGGCCCA GTTCTGTAGG ATGCAAGAAA AACGCATCAT CAAGAGACAG
    GATGTGATTT ACGAGTTCAT GCAGACCGAG CTCCATCACG TGCAGACACT CATGGTGATG
    GCAGAGGTCT TCAGAAGGGG AATGCGGGAT GAAGTGGGTC TGGACGCAGA GACCATTGGC
    CGGATTTTTC CTTGCTTAGA TGAGCTGCTC CAACTCCATC GTGATTTTCT GACAGCAATG
    AGGGAACGAA GGCAAAGCTG CGTCCAGAAC AGCAGCAGCA AAAACTTTCT CATCCACCGT
    GTGGGTGATA TTTTTCTGCA ACAGTTCTCC CAGGAGAACG CAGAGAAGAT GAAGCAGGTG
    TATGGAGAGT TTTGCAGTCA TCACACAGAA GCAGTCAGTT ACTTCAAAGA GCTTCAGCAG
    CAGAACAAGA GATTTCAGCT CTTCATCAAA CAACAGAGTA GTAACTCACT GGTGAAGAGG
    CGAGAGATCC CAGAATGTAT TTTGTTAGTG ACGCAGCGAA TTACAAAGTA CCCTGTCCTC
    CTAGAGAGAA TACTCCAGTA CACAGACGAG GGCACAGAAG AGCACTCTGA CCTCTCTCGA
    GCTCTAGTCC TGATCCGAGA GCTGATCTCC GCTGTGGACC AATATGTGAG TCAGTATGAG
    CAGAACCAGA AGCTGAGTGA AGTCTTGGGC CGCATGGAGA ACAAGTGCTC TACCAAACTG
    AAGACCGGCT GCACTTTCCG CAAGCAGGAC ATCACCAGCG GCAGGAATCT GCTGCACCAG
    GGCCCGCTGC TCTGGAAGAC AGCCACTGGA AGACTGAAAG ATGTTCTGGC ATTTTTGCTG
    TCTGATTCAC TGGTGTTCCT TCAGGAGAAA GATCAGAAAT ACATTTTTGC TACTGTGGAC
    CAGAAGCCTC CAGTGATCTC TCTGCAGAAA CTAATTGTGC GTGAGGTGGC CAATGAAGAG
    AGAGGCATGT TTCTGATCAG TGCATCTTCA GCCGGGCCTG AGATGTACGA GGTGCACGCA
    GCCTCTAAAG ATGAACGCAA CGCCTGGATG AGACTCATCA GAGAGGCCGT GGAGAATTGC
    CCTGAAGAGG AGGATGAAAC TACAAGTGAG TCAGAAGAGG AAAGGAGAGT CACAGAAGCA
    AAGGTCCAAA AGATCCACCG GCTGCAAGAG ACGCTGTGTG GCCATGATCA CCTCATCTGT
    TCCAATCTGG AGGAGAAGCT GCACATCTAT GCCGAGCTGT CAGTGATGGC AGGCCAGCGA
    GAGGCAGGAC CAGAGCCCAG GCTGCTGGTA CGACCCAGCA CTGAAGACGT TCCCCAGGCT
    GGAGTACTAC TCAGCGCCGC TCTCAGAGAG GCGGAGAGCC TAAGAGCAGC TCTCTCCTCT
    CATTGTGCTT CCACTCCTCC CGTCCCGTCC GGTCCAGAAG ATCTAAACAA ACCTGAACCC
    TGCACTCCTC CATCTCCTCC TCTGCCTCCT GAAAGTCCAC TGACAGACCC CATCCAGAGT
    GAGGAGGAGG AGTGGAGAAC TGAGGAAAGC TCAGAAGCAC AGACCTTTAA TCACAACTCG
    GACTCTGACC ACAATCTACA GCTGAAGGTG AGTCAGAGTG TGCAGAGCTT GACTCAGCTG
    CTGTACAGTC TACAGGCTGC AGTTACCATC CAAGACAGTT GCTATGAAGT GCAGAAGCTT
    CTTCTCCTGC TGATGTCAAG CCCCGCCCCT AACCAACAGT CCCGCCCACT ATCCATCACC
    CGCAGCGGGC AGGCTCTGCA TGAGCAGGAG CGCCAGCGGG AGGCTGAGCG CCGCCGGGAA
    GAGCTGGCGG ATGTGGCTCG TCTGCGGGGA GCCCTGGTAC GAGAGCGCCA GCAGTGGGAG
    AGGGAGTGCC AGGCGCGGCA GCTTCAGCAC TCAGAACTGG AGACCACGCT TGAGCAGAGG
    GAGCTCTTGT GCCACCTAGA GGAGCGGAGA CTGCAGAGCG AGCGGCAGGA GCTCCAGGAG
    CAGCTGCAGG AGTACCAACA GAGTCTGGAG AGGCTCAGAG AAGGCCAGAG GAATGTGGAG
    AAGGAGAAGG AGAAACTGGA GACTCAGAAG ATGCTGCTGG AGGGCTTGAA GCACGGGTGC
    CAGCAGAACC TTCCAGCTAT GGTCATACCA CTGGACGGAC AGCAGACACA GAATCACCTG
    GGCATTCAGG ACGAGCATGG CTCTATTTTT GTCAATGAGG CAGCGTTCAT ATCTTCTTCT
    GTAAATAACA GGCACCTCCA TCTTCAGCAT CATCCACAGC ATCATCACCA GACTGTCCAT
    GTGCACTCAG GATCTATAGG ATACTCAGAT TCTCCCAGCG CTCAGAACAG TCTTAATTCA
    CTGCTAGCCC GATCCAACCA CAGACCCAGC TCTGCTGGAG CGTCCGACCA CAGCTGGACG
    TCTCTGAGGA GTCTGACAGA GAGTGCAAAC AACAATGTGG GTCAGATCAG CCATGTTGAA
    GAGTCTTGGT CAGCAGGGGT AACGGGAGCG GGACTCCATC AAACTGATGT CCTGCTCGTC
    AGCCAGCCCA TCCTAAGCAT GGAGACAGAG AACAGGGATA CTGATGGAGA GGAGACCATC
    GTGTATCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144579
    Clone ID Related Accession (Same CDS sequence) XM_017356272.2
    Accession Version XM_017356272.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394590.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAGC AGCCTCAAGA GAAAAGGATT
    GAGGAAGAAG AATGGGATAA ATATATCATC CCTTCCAAAA CAGAGTCAGA GAAGTACAAA
    GTCAGCCGCA CATTCAGCTT CATAAAGAGC CGAATGTATA GCACTCGCAA CAAGAACAAG
    GGGAAAGGAA AAGAGAAAGA GACCAAAGAC AAGCAGGTCA ACGGACACCA GTTCACAGCG
    GGATCTGTCC TGGGGTCGAC ACTCTGTGAG ATCTGCAGCA AGCCAGCTTC AGGGAAAGAG
    GTTGTCCATT GCACACACTG TGCAGTGAGT GTCCATAAGG GCTGTAAGGA CTCATCTACG
    CCATGCCTGA AGAAACCTCA AGACAAATAT TCAGTGATGA CAGTAAAAAG CAAGACAGCC
    TCTCTTCCTC AAAATTTTAT TGTGCGTGAG AGTTCATCGT CGTCCCTTCA GATCTCTACC
    TCTGCTTCTC TCCCTGTCAT GACATCACGA GACTCGGCTC CTCTTCCTTG TCCTCTGTCC
    AGAAGTGTGC CCTTAACCAC TGAAAGGTTT AGCGAGAGCC CAGATGCTGA TAGTGATTCT
    CCTCTTTGGA GGAACCACAT CCAATCAGAG GAGCTTCTCC AAACCATGGA GTCCTCAACA
    TCCACAGACT CGTCCATCAT TGAAGACTCG GTTGATTCCC CTTTACAGGT TGAGCTGCTG
    ATGAATGCTG TAGATTTGGA GGCAGAGTCA TGGAGTTTAG CTGTGGAGGC CCAGTTCTGT
    AGGATGCAAG AAAAACGCAT CATCAAGAGA CAGGATGTGA TTTACGAGTT CATGCAGACC
    GAGCTCCATC ACGTGCAGAC ACTCATGGTG ATGGCAGAGG TCTTCAGAAG GGGAATGCGG
    GATGAAGTGG GTCTGGACGC AGAGACCATT GGCCGGATTT TTCCTTGCTT AGATGAGCTG
    CTCCAACTCC ATCGTGATTT TCTGACAGCA ATGAGGGAAC GAAGGCAAAG CTGCGTCCAG
    AACAGCAGCA GCAAAAACTT TCTCATCCAC CGTGTGGGTG ATATTTTTCT GCAACAGTTC
    TCCCAGGAGA ACGCAGAGAA GATGAAGCAG GTGTATGGAG AGTTTTGCAG TCATCACACA
    GAAGCAGTCA GTTACTTCAA AGAGCTTCAG CAGCAGAACA AGAGATTTCA GCTCTTCATC
    AAACAACAGA GTAGTAACTC ACTGGTGAAG AGGCGAGAGA TCCCAGAATG TATTTTGTTA
    GTGACGCAGC GAATTACAAA GTACCCTGTC CTCCTAGAGA GAATACTCCA GTACACAGAC
    GAGGGCACAG AAGAGCACTC TGACCTCTCT CGAGCTCTAG TCCTGATCCG AGAGCTGATC
    TCCGCTGTGG ACCAATATGT GAGTCAGTAT GAGCAGAACC AGAAGCTGAG TGAAGTCTTG
    GGCCGCATGG AGAACAAGTG CTCTACCAAA CTGAAGACCG GCTGCACTTT CCGCAAGCAG
    GACATCACCA GCGGCAGGAA TCTGCTGCAC CAGGGCCCGC TGCTCTGGAA GACAGCCACT
    GGAAGACTGA AAGATGTTCT GGCATTTTTG CTGTCTGATT CACTGGTGTT CCTTCAGGAG
    AAAGATCAGA AATACATTTT TGCTACTGTG GACCAGAAGC CTCCAGTGAT CTCTCTGCAG
    AAACTAATTG TGCGTGAGGT GGCCAATGAA GAGAGAGGCA TGTTTCTGAT CAGTGCATCT
    TCAGCCGGGC CTGAGATGTA CGAGGTGCAC GCAGCCTCTA AAGATGAACG CAACGCCTGG
    ATGAGACTCA TCAGAGAGGC CGTGGAGAAT TGCCCTGAAG AGGAGGATGA AACTACAAGT
    GAGTCAGAAG AGGAAAGGAG AGTCACAGAA GCAAAGGTCC AAAAGATCCA CCGGCTGCAA
    GAGACGCTGT GTGGCCATGA TCACCTCATC TGTTCCAATC TGGAGGAGAA GCTGCACATC
    TATGCCGAGC TGTCAGTGAT GGCAGGCCAG CGAGAGGCAG GACCAGAGCC CAGGCTGCTG
    GTACGACCCA GCACTGAAGA CGTTCCCCAG GCTGGAGTAC TACTCAGCGC CGCTCTCAGA
    GAGGCGGAGA GCCTAAGAGC AGCTCTCTCC TCTCATTGTG CTTCCACTCC TCCCGTCCCG
    TCCGGTCCAG AAGATCTAAA CAAACCTGAA CCCTGCACTC CTCCATCTCC TCCTCTGCCT
    CCTGAAAGTC CACTGACAGA CCCCATCCAG AGTGAGGAGG AGGAGTGGAG AACTGAGGAA
    AGCTCAGAAG CACAGACCTT TAATCACAAC TCGGACTCTG ACCACAATCT ACAGCTGAAG
    GTGAGTCAGA GTGTGCAGAG CTTGACTCAG CTGCTGTACA GTCTACAGGC TGCAGTTACC
    ATCCAAGACA GTTGCTATGA AGTGCAGAAG CTTCTTCTCC TGCTGATGTC AAGCCCCGCC
    CCTAACCAAC AGTCCCGCCC ACTATCCATC ACCCGCAGCG GGCAGGCTCT GCATGAGCAG
    GAGCGCCAGC GGGAGGCTGA GCGCCGCCGG GAAGAGCTGG CGGATGTGGC TCGTCTGCGG
    GGAGCCCTGG TACGAGAGCG CCAGCAGTGG GAGAGGGAGT GCCAGGCGCG GCAGCTTCAG
    CACTCAGAAC TGGAGACCAC GCTTGAGCAG AGGGAGCTCT TGTGCCACCT AGAGGAGCGG
    AGACTGCAGA GCGAGCGGCA GGAGCTCCAG GAGCAGCTGC AGGAGTACCA ACAGAGTCTG
    GAGAGGCTCA GAGAAGGCCA GAGGAATGTG GAGAAGGAGA AGGAGAAACT GGAGACTCAG
    AAGATGCTGC TGGAGGGCTT GAAGCACGGG TGCCAGCAGA ACCTTCCAGC TATGGTCATA
    CCACTGGACG GACAGCAGAC ACAGAATCAC CTGGGCATTC AGGACGAGCA TGGCTCTATT
    TTTGTCAATG AGGCAGCGTT CATATCTTCT TCTGTAAATA ACAGGCACCT CCATCTTCAG
    CATCATCCAC AGCATCATCA CCAGACTGTC CATGTGCACT CAGGATCTAT AGGATACTCA
    GATTCTCCCA GCGCTCAGAA CAGTCTTAAT TCACTGCTAG CCCGATCCAA CCACAGACCC
    AGCTCTGCTG GAGCGTCCGA CCACAGCTGG ACGTCTCTGA GGAGTCTGAC AGAGAGTGCA
    AACAACAATG TGGGTCAGAT CAGCCATGTT GAAGAGTCTT GGTCAGCAGG GGTAACGGGA
    GCGGGACTCC ATCAAACTGA TGTCCTGCTC GTCAGCCAGC CCATCCTAAG CATGGAGACA
    GAGAACAGGG ATACTGATGG AGAGGAGACC ATCGTGTATC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211761.2
    CDS207..5471
    Translation

    Target ORF information:

    RefSeq Version XM_017356272.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356272.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCTGCT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG CACCCCGCCT TTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAGC AGCCTCAAGA GAAAAGGATT
    GAGGAAGAAG AATGGGATAA ATATATCATC CCTTCCAAAA CAGAGTCAGA GAAGTACAAA
    GTCAGCCGCA CATTCAGCTT CATAAAGAGC CGAATGTATA GCACTCGCAA CAAGAACAAG
    GGGAAAGGAA AAGAGAAAGA GACCAAAGAC AAGCAGGTCA ACGGACACCA GTTCACAGCG
    GGATCTGTCC TGGGGTCGAC ACTCTGTGAG ATCTGCAGCA AGCCAGCTTC AGGGAAAGAG
    GTTGTCCATT GCACACACTG TGCAGTGAGT GTCCATAAGG GCTGTAAGGA CTCATCTACG
    CCATGCCTGA AGAAACCTCA AGACAAATAT TCAGTGATGA CAGTAAAAAG CAAGACAGCC
    TCTCTTCCTC AAAATTTTAT TGTGCGTGAG AGTTCATCGT CGTCCCTTCA GATCTCTACC
    TCTGCTTCTC TCCCTGTCAT GACATCACGA GACTCGGCTC CTCTTCCTTG TCCTCTGTCC
    AGAAGTGTGC CCTTAACCAC TGAAAGGTTT AGCGAGAGCC CAGATGCTGA TAGTGATTCT
    CCTCTTTGGA GGAACCACAT CCAATCAGAG GAGCTTCTCC AAACCATGGA GTCCTCAACA
    TCCACAGACT CGTCCATCAT TGAAGACTCG GTTGATTCCC CTTTACAGGT TGAGCTGCTG
    ATGAATGCTG TAGATTTGGA GGCAGAGTCA TGGAGTTTAG CTGTGGAGGC CCAGTTCTGT
    AGGATGCAAG AAAAACGCAT CATCAAGAGA CAGGATGTGA TTTACGAGTT CATGCAGACC
    GAGCTCCATC ACGTGCAGAC ACTCATGGTG ATGGCAGAGG TCTTCAGAAG GGGAATGCGG
    GATGAAGTGG GTCTGGACGC AGAGACCATT GGCCGGATTT TTCCTTGCTT AGATGAGCTG
    CTCCAACTCC ATCGTGATTT TCTGACAGCA ATGAGGGAAC GAAGGCAAAG CTGCGTCCAG
    AACAGCAGCA GCAAAAACTT TCTCATCCAC CGTGTGGGTG ATATTTTTCT GCAACAGTTC
    TCCCAGGAGA ACGCAGAGAA GATGAAGCAG GTGTATGGAG AGTTTTGCAG TCATCACACA
    GAAGCAGTCA GTTACTTCAA AGAGCTTCAG CAGCAGAACA AGAGATTTCA GCTCTTCATC
    AAACAACAGA GTAGTAACTC ACTGGTGAAG AGGCGAGAGA TCCCAGAATG TATTTTGTTA
    GTGACGCAGC GAATTACAAA GTACCCTGTC CTCCTAGAGA GAATACTCCA GTACACAGAC
    GAGGGCACAG AAGAGCACTC TGACCTCTCT CGAGCTCTAG TCCTGATCCG AGAGCTGATC
    TCCGCTGTGG ACCAATATGT GAGTCAGTAT GAGCAGAACC AGAAGCTGAG TGAAGTCTTG
    GGCCGCATGG AGAACAAGTG CTCTACCAAA CTGAAGACCG GCTGCACTTT CCGCAAGCAG
    GACATCACCA GCGGCAGGAA TCTGCTGCAC CAGGGCCCGC TGCTCTGGAA GACAGCCACT
    GGAAGACTGA AAGATGTTCT GGCATTTTTG CTGTCTGATT CACTGGTGTT CCTTCAGGAG
    AAAGATCAGA AATACATTTT TGCTACTGTG GACCAGAAGC CTCCAGTGAT CTCTCTGCAG
    AAACTAATTG TGCGTGAGGT GGCCAATGAA GAGAGAGGCA TGTTTCTGAT CAGTGCATCT
    TCAGCCGGGC CTGAGATGTA CGAGGTGCAC GCAGCCTCTA AAGATGAACG CAACGCCTGG
    ATGAGACTCA TCAGAGAGGC CGTGGAGAAT TGCCCTGAAG AGGAGGATGA AACTACAAGT
    GAGTCAGAAG AGGAAAGGAG AGTCACAGAA GCAAAGGTCC AAAAGATCCA CCGGCTGCAA
    GAGACGCTGT GTGGCCATGA TCACCTCATC TGTTCCAATC TGGAGGAGAA GCTGCACATC
    TATGCCGAGC TGTCAGTGAT GGCAGGCCAG CGAGAGGCAG GACCAGAGCC CAGGCTGCTG
    GTACGACCCA GCACTGAAGA CGTTCCCCAG GCTGGAGTAC TACTCAGCGC CGCTCTCAGA
    GAGGCGGAGA GCCTAAGAGC AGCTCTCTCC TCTCATTGTG CTTCCACTCC TCCCGTCCCG
    TCCGGTCCAG AAGATCTAAA CAAACCTGAA CCCTGCACTC CTCCATCTCC TCCTCTGCCT
    CCTGAAAGTC CACTGACAGA CCCCATCCAG AGTGAGGAGG AGGAGTGGAG AACTGAGGAA
    AGCTCAGAAG CACAGACCTT TAATCACAAC TCGGACTCTG ACCACAATCT ACAGCTGAAG
    GTGAGTCAGA GTGTGCAGAG CTTGACTCAG CTGCTGTACA GTCTACAGGC TGCAGTTACC
    ATCCAAGACA GTTGCTATGA AGTGCAGAAG CTTCTTCTCC TGCTGATGTC AAGCCCCGCC
    CCTAACCAAC AGTCCCGCCC ACTATCCATC ACCCGCAGCG GGCAGGCTCT GCATGAGCAG
    GAGCGCCAGC GGGAGGCTGA GCGCCGCCGG GAAGAGCTGG CGGATGTGGC TCGTCTGCGG
    GGAGCCCTGG TACGAGAGCG CCAGCAGTGG GAGAGGGAGT GCCAGGCGCG GCAGCTTCAG
    CACTCAGAAC TGGAGACCAC GCTTGAGCAG AGGGAGCTCT TGTGCCACCT AGAGGAGCGG
    AGACTGCAGA GCGAGCGGCA GGAGCTCCAG GAGCAGCTGC AGGAGTACCA ACAGAGTCTG
    GAGAGGCTCA GAGAAGGCCA GAGGAATGTG GAGAAGGAGA AGGAGAAACT GGAGACTCAG
    AAGATGCTGC TGGAGGGCTT GAAGCACGGG TGCCAGCAGA ACCTTCCAGC TATGGTCATA
    CCACTGGACG GACAGCAGAC ACAGAATCAC CTGGGCATTC AGGACGAGCA TGGCTCTATT
    TTTGTCAATG AGGCAGCGTT CATATCTTCT TCTGTAAATA ACAGGCACCT CCATCTTCAG
    CATCATCCAC AGCATCATCA CCAGACTGTC CATGTGCACT CAGGATCTAT AGGATACTCA
    GATTCTCCCA GCGCTCAGAA CAGTCTTAAT TCACTGCTAG CCCGATCCAA CCACAGACCC
    AGCTCTGCTG GAGCGTCCGA CCACAGCTGG ACGTCTCTGA GGAGTCTGAC AGAGAGTGCA
    AACAACAATG TGGGTCAGAT CAGCCATGTT GAAGAGTCTT GGTCAGCAGG GGTAACGGGA
    GCGGGACTCC ATCAAACTGA TGTCCTGCTC GTCAGCCAGC CCATCCTAAG CATGGAGACA
    GAGAACAGGG ATACTGATGG AGAGGAGACC ATCGTGTATC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59219
    Clone ID Related Accession (Same CDS sequence) XM_021476529.1 , XM_017356269.1
    Accession Version XM_021476529.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGTTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAACAGC CTCAAGAGAA AAGGGAGTTA AGGTTTCGCA GGCGAGCTCA GTCGGCTGAT
    GATGAAAGCA GTGCAGAGCT GGCCGACTCG CTCCAACATC TCACGCTCTC AGAGTTCCTC
    AAAGAGATTG AGGAAGAAGA ATGGGATAAA TATATCATCC CTTCCAAAAC AGAGTCAGAA
    AAGTACAAAG TCAGCCGCAC ATTCAGCTTC ATAAAGAGCC GAATGTATAG CACTCGCAAC
    AAGAACAAGG GGAAAGGAAA AGAGAAAGAG ACCAAAGACA AGCAGGTCAA CGGACACCAG
    TTCACAGCGG GATCTGTCCT GGGGTCGACA CTCTGTGAGA TCTGCAGCAA GCCAGCTTCA
    GGGAAAGAGG TTGTCCATTG CACACACTGT GCAGTGAGTG TCCATAAGGG CTGTAAGGAC
    TCATCTACGC CATGCCTGAA GAAACCTCAA GACAAATATT CAGTGATGAC AGTAAAAAGC
    AAGACAGCCT CTCTTCCTCA AAATTTTATT GTGCGTGAGA GTTCATCGTC GTCCCTTCAG
    ATCTCTACCT CTGCTTCTCT CCCTGTGATG ACATCACGAG ACTCGGCTCC TCTTCCTTGT
    CCTCTGTCCA GAAGTGTGCC CTTAACCACT GAAAGGCTTA GCGAGAGCCC AGAAGCTGAT
    AGTGATTCTA ATCTTTGGAG GAACCACATC CAATCAGAGG AGCTTCTCCA AACCATGGAG
    TCCTCAACAT CCACAGACTC GTCCATCATT GAAGACTCGG TTGATTCCCC TTTACAGATT
    GAGCTGCTGA TGAATGCTGT AGATTTGGAG GCAGAGTCAT GGAGTTTAGC TGTGGAGGCC
    CAGTTCTGTA GGATGCAAGA AAAACGCATC ATCAAGAGAC AGGATGTGAT TTACGAGTTC
    ATGCAGACCG AGCTCCATCA CGTGCAGACA CTCATGGTGA TGGCAGAGGT CTTCAGAAGG
    GGAATGCGGG ATGAAGTGGG TCTGGACGCA GAGACCATTG GCCGGATTTT TCCTTGCTTA
    GATGAGCTAC TCCAACTCCA TCGTGATTTT CTGACAGCAA TGAGGGAACG AAGGCAAAGC
    TGCGTCCAGA ACAACAGCAG CAAAAACTTT CTCATCCACC GTGTGGGTGA TATTTTTCTG
    CAACAGTTCT CCCAGGAGAA CGCAGAGAAG ATGAAGCAGG TGTATGGAGA GTTTTGCAGT
    CATCACACAG AAGCAGTCAG TTACTTCAAA GAGCTTCAGC AGCAGAACAA GAGATTTCAG
    CTCTTCATCA AACAACAGAG TAGTAACTCG CTGGTGAAGA GGCGAGAGAT CCCAGAATGT
    ATTTTGTTAG TGACGCAGCG AATTACAAAG TACCCTGTCC TCCTAGAGAG AATACTCCAG
    TACACAGACG AGGGCACAGA AGAGCACTCT GACCTCTCTC GAGCTCTAGT CCTGATCCGA
    GAGCTGATCT CCGCTGTGGA CCAATATGTG AGTCAGTATG AGCAGAACCA GAAGCTGAGT
    GAAGTCTTGG GCCGCATGGA GAACAAGTGC TCTACCAAAC TGAAGACCGG CTGCACTTTC
    CGCAAGCAGG ACATCACCAG CGGCAGGAAT CTGCTGCACC AGGGCCCGCT GCTCTGGAAG
    ACAGCCACTG GAAGACTGAA AGATGTTCTG GCATTTTTGC TGTCTGATTC ACTGGTGTTC
    CTTCAGGAGA AAGATCAGAA ATACATTTTT GCTACTGTGG ACCAGAAGCC TCCAGTGATC
    TCTTTGCAGA AACTAATTGT GCGTGAGGTG GCCAATGAAG AGAGAGGCAT GTTTCTGATC
    AGTGCATCTT CAGCCGGGCC TGAGATGTAC GAGGTGCATG CAGCCTCTAA AGATGAACGC
    AACGCCTGGA TGAGACTCAT CAGAGAGGCC GTGGAGAATT GCCCTGAAGA GGAGGATGAA
    ACTACAAGTG AGTCAGAAGA GGAAAGGAGA GTCACAGAAG CAAAGGTCCA AAAGATCCAC
    CGGCTGCAAG AGACGCTGTG TGGCCATGAT CACCTCATCT GTTCCAATCT GGAGGAGAAG
    CTGCACATCT ATGCCGAGCT GTCAGTGATG GCAGGCCAGC GAGAGGCAGG ACCAGAGCCC
    AGGCTGCTGG TACGACCCAG CACTGAAGAC GTTCCCCAGG CTGGAGTACT ACTCAGCGCC
    GCTCTCAGAG AGGCGGAGAG CCTAAGAGCA GCTCTCTCCT CTCATTGTGC TTCCACTCCT
    CCCGTCCCGT CCGGTCCAGA AGATCTAAAC AAACCTGAAC CCTGCACTCC TCCATCTCCT
    CTGCCTCCTG AAAGTCCACT GACAGACCCC ATCCAGAGTG AGGAGGAGGA GTGGAGAACT
    GAGGAAAGCT CAGAAGCACA GACCTTTAAT CACAACTCGG ACTCTGACCA CAATCTACAG
    CTGAAGGTGA GTCAGAGTGT GCAGAGCTTG ACTCAGCTGC TGTACAGTCT ACAGGCTGCA
    GTTACCATCC AAGACAGTTG CTATGAAGTG CAGAAGCTTC TTCTCCTGCT GATGTCAAGC
    CCCGCCCCTA ACCAACAGTC CCGCCCACTA TCCATCACCC GCAGCGGGCA GGCTCTGCAT
    GAGCAGGAGC GCCAGCGGGA GGCCGAGCGC CGCCGGGAAG AGCTGGCGGA TGTGGCTCGT
    CTGCGGGGAG CCCTGGTACG AGAGCGCCAG CAGTGGGAGA GGGAGTGCCA GGCGCGGCAG
    CTTCAGCACT CAGAACTGGA GACCACGCTT GAGCAGAGGG AGCTCTTGTG CCACCTAGAG
    GAGCGGAGGC TGCAGAGCGA GCGGCAGGAG CTCCAGGAGC AGCTGCAGGA ATACCAACAG
    AGTCTGGAGA GGCTCAGAGA AGGCCAGAGG AATGTGGAGA AGGAGAAGGA GAAACTGGAG
    ACTCAGAAGA TGCTGCTGGA GGGCTTGAAG CACGGGTGCC AGCAGAACCT TCCAGCTATG
    GTCATACCAC TGGACGGACA GCAGACACAG AATCACCTGG GCATTCAGGA CGAGCATGGC
    TCTATTTTTG TCAATGAGGC AGCGTTCATA TCTTCTTCTG TAAATAACAG GCACCTCCAT
    CTTCAGCATC ATCCACAGCA TCATCACCAG ACTGTCCATG TGCACTCAGG ATCTATAGGA
    TACTCAGATT CTCCCAGCGC TCAGAACAGT CTTAATTCAC TGCTAGCCCG ATCCAACCAC
    AGACCCAGCT CTGCTGGAGC ATCCGACCAC AGCTGGACGT CTCTGAGGAG TCTGACAGAG
    AGTGCAAACA ACAATGTGGG TCAGATCAGC CATGTTGAAG AGTCTTGGTC AGCAGGGGTA
    ACGGGAGCGG GACTCCATCA AACTGATGTC CTGCTCGTCA GCCAGCCCTT CCTAAGCATG
    GAGACAGAGA ACAGAGATAC TGATGGAGAG GAGACCATCG TGTATCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332204.1
    CDS206..5656
    Translation

    Target ORF information:

    RefSeq Version XM_021476529.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGTTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAACAGC CTCAAGAGAA AAGGGAGTTA AGGTTTCGCA GGCGAGCTCA GTCGGCTGAT
    GATGAAAGCA GTGCAGAGCT GGCCGACTCG CTCCAACATC TCACGCTCTC AGAGTTCCTC
    AAAGAGATTG AGGAAGAAGA ATGGGATAAA TATATCATCC CTTCCAAAAC AGAGTCAGAA
    AAGTACAAAG TCAGCCGCAC ATTCAGCTTC ATAAAGAGCC GAATGTATAG CACTCGCAAC
    AAGAACAAGG GGAAAGGAAA AGAGAAAGAG ACCAAAGACA AGCAGGTCAA CGGACACCAG
    TTCACAGCGG GATCTGTCCT GGGGTCGACA CTCTGTGAGA TCTGCAGCAA GCCAGCTTCA
    GGGAAAGAGG TTGTCCATTG CACACACTGT GCAGTGAGTG TCCATAAGGG CTGTAAGGAC
    TCATCTACGC CATGCCTGAA GAAACCTCAA GACAAATATT CAGTGATGAC AGTAAAAAGC
    AAGACAGCCT CTCTTCCTCA AAATTTTATT GTGCGTGAGA GTTCATCGTC GTCCCTTCAG
    ATCTCTACCT CTGCTTCTCT CCCTGTGATG ACATCACGAG ACTCGGCTCC TCTTCCTTGT
    CCTCTGTCCA GAAGTGTGCC CTTAACCACT GAAAGGCTTA GCGAGAGCCC AGAAGCTGAT
    AGTGATTCTA ATCTTTGGAG GAACCACATC CAATCAGAGG AGCTTCTCCA AACCATGGAG
    TCCTCAACAT CCACAGACTC GTCCATCATT GAAGACTCGG TTGATTCCCC TTTACAGATT
    GAGCTGCTGA TGAATGCTGT AGATTTGGAG GCAGAGTCAT GGAGTTTAGC TGTGGAGGCC
    CAGTTCTGTA GGATGCAAGA AAAACGCATC ATCAAGAGAC AGGATGTGAT TTACGAGTTC
    ATGCAGACCG AGCTCCATCA CGTGCAGACA CTCATGGTGA TGGCAGAGGT CTTCAGAAGG
    GGAATGCGGG ATGAAGTGGG TCTGGACGCA GAGACCATTG GCCGGATTTT TCCTTGCTTA
    GATGAGCTAC TCCAACTCCA TCGTGATTTT CTGACAGCAA TGAGGGAACG AAGGCAAAGC
    TGCGTCCAGA ACAACAGCAG CAAAAACTTT CTCATCCACC GTGTGGGTGA TATTTTTCTG
    CAACAGTTCT CCCAGGAGAA CGCAGAGAAG ATGAAGCAGG TGTATGGAGA GTTTTGCAGT
    CATCACACAG AAGCAGTCAG TTACTTCAAA GAGCTTCAGC AGCAGAACAA GAGATTTCAG
    CTCTTCATCA AACAACAGAG TAGTAACTCG CTGGTGAAGA GGCGAGAGAT CCCAGAATGT
    ATTTTGTTAG TGACGCAGCG AATTACAAAG TACCCTGTCC TCCTAGAGAG AATACTCCAG
    TACACAGACG AGGGCACAGA AGAGCACTCT GACCTCTCTC GAGCTCTAGT CCTGATCCGA
    GAGCTGATCT CCGCTGTGGA CCAATATGTG AGTCAGTATG AGCAGAACCA GAAGCTGAGT
    GAAGTCTTGG GCCGCATGGA GAACAAGTGC TCTACCAAAC TGAAGACCGG CTGCACTTTC
    CGCAAGCAGG ACATCACCAG CGGCAGGAAT CTGCTGCACC AGGGCCCGCT GCTCTGGAAG
    ACAGCCACTG GAAGACTGAA AGATGTTCTG GCATTTTTGC TGTCTGATTC ACTGGTGTTC
    CTTCAGGAGA AAGATCAGAA ATACATTTTT GCTACTGTGG ACCAGAAGCC TCCAGTGATC
    TCTTTGCAGA AACTAATTGT GCGTGAGGTG GCCAATGAAG AGAGAGGCAT GTTTCTGATC
    AGTGCATCTT CAGCCGGGCC TGAGATGTAC GAGGTGCATG CAGCCTCTAA AGATGAACGC
    AACGCCTGGA TGAGACTCAT CAGAGAGGCC GTGGAGAATT GCCCTGAAGA GGAGGATGAA
    ACTACAAGTG AGTCAGAAGA GGAAAGGAGA GTCACAGAAG CAAAGGTCCA AAAGATCCAC
    CGGCTGCAAG AGACGCTGTG TGGCCATGAT CACCTCATCT GTTCCAATCT GGAGGAGAAG
    CTGCACATCT ATGCCGAGCT GTCAGTGATG GCAGGCCAGC GAGAGGCAGG ACCAGAGCCC
    AGGCTGCTGG TACGACCCAG CACTGAAGAC GTTCCCCAGG CTGGAGTACT ACTCAGCGCC
    GCTCTCAGAG AGGCGGAGAG CCTAAGAGCA GCTCTCTCCT CTCATTGTGC TTCCACTCCT
    CCCGTCCCGT CCGGTCCAGA AGATCTAAAC AAACCTGAAC CCTGCACTCC TCCATCTCCT
    CTGCCTCCTG AAAGTCCACT GACAGACCCC ATCCAGAGTG AGGAGGAGGA GTGGAGAACT
    GAGGAAAGCT CAGAAGCACA GACCTTTAAT CACAACTCGG ACTCTGACCA CAATCTACAG
    CTGAAGGTGA GTCAGAGTGT GCAGAGCTTG ACTCAGCTGC TGTACAGTCT ACAGGCTGCA
    GTTACCATCC AAGACAGTTG CTATGAAGTG CAGAAGCTTC TTCTCCTGCT GATGTCAAGC
    CCCGCCCCTA ACCAACAGTC CCGCCCACTA TCCATCACCC GCAGCGGGCA GGCTCTGCAT
    GAGCAGGAGC GCCAGCGGGA GGCCGAGCGC CGCCGGGAAG AGCTGGCGGA TGTGGCTCGT
    CTGCGGGGAG CCCTGGTACG AGAGCGCCAG CAGTGGGAGA GGGAGTGCCA GGCGCGGCAG
    CTTCAGCACT CAGAACTGGA GACCACGCTT GAGCAGAGGG AGCTCTTGTG CCACCTAGAG
    GAGCGGAGGC TGCAGAGCGA GCGGCAGGAG CTCCAGGAGC AGCTGCAGGA ATACCAACAG
    AGTCTGGAGA GGCTCAGAGA AGGCCAGAGG AATGTGGAGA AGGAGAAGGA GAAACTGGAG
    ACTCAGAAGA TGCTGCTGGA GGGCTTGAAG CACGGGTGCC AGCAGAACCT TCCAGCTATG
    GTCATACCAC TGGACGGACA GCAGACACAG AATCACCTGG GCATTCAGGA CGAGCATGGC
    TCTATTTTTG TCAATGAGGC AGCGTTCATA TCTTCTTCTG TAAATAACAG GCACCTCCAT
    CTTCAGCATC ATCCACAGCA TCATCACCAG ACTGTCCATG TGCACTCAGG ATCTATAGGA
    TACTCAGATT CTCCCAGCGC TCAGAACAGT CTTAATTCAC TGCTAGCCCG ATCCAACCAC
    AGACCCAGCT CTGCTGGAGC ATCCGACCAC AGCTGGACGT CTCTGAGGAG TCTGACAGAG
    AGTGCAAACA ACAATGTGGG TCAGATCAGC CATGTTGAAG AGTCTTGGTC AGCAGGGGTA
    ACGGGAGCGG GACTCCATCA AACTGATGTC CTGCTCGTCA GCCAGCCCTT CCTAAGCATG
    GAGACAGAGA ACAGAGATAC TGATGGAGAG GAGACCATCG TGTATCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59219
    Clone ID Related Accession (Same CDS sequence) XM_021476529.1 , XM_017356269.1
    Accession Version XM_017356269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGTTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAACAGC CTCAAGAGAA AAGGGAGTTA AGGTTTCGCA GGCGAGCTCA GTCGGCTGAT
    GATGAAAGCA GTGCAGAGCT GGCCGACTCG CTCCAACATC TCACGCTCTC AGAGTTCCTC
    AAAGAGATTG AGGAAGAAGA ATGGGATAAA TATATCATCC CTTCCAAAAC AGAGTCAGAA
    AAGTACAAAG TCAGCCGCAC ATTCAGCTTC ATAAAGAGCC GAATGTATAG CACTCGCAAC
    AAGAACAAGG GGAAAGGAAA AGAGAAAGAG ACCAAAGACA AGCAGGTCAA CGGACACCAG
    TTCACAGCGG GATCTGTCCT GGGGTCGACA CTCTGTGAGA TCTGCAGCAA GCCAGCTTCA
    GGGAAAGAGG TTGTCCATTG CACACACTGT GCAGTGAGTG TCCATAAGGG CTGTAAGGAC
    TCATCTACGC CATGCCTGAA GAAACCTCAA GACAAATATT CAGTGATGAC AGTAAAAAGC
    AAGACAGCCT CTCTTCCTCA AAATTTTATT GTGCGTGAGA GTTCATCGTC GTCCCTTCAG
    ATCTCTACCT CTGCTTCTCT CCCTGTGATG ACATCACGAG ACTCGGCTCC TCTTCCTTGT
    CCTCTGTCCA GAAGTGTGCC CTTAACCACT GAAAGGCTTA GCGAGAGCCC AGAAGCTGAT
    AGTGATTCTA ATCTTTGGAG GAACCACATC CAATCAGAGG AGCTTCTCCA AACCATGGAG
    TCCTCAACAT CCACAGACTC GTCCATCATT GAAGACTCGG TTGATTCCCC TTTACAGATT
    GAGCTGCTGA TGAATGCTGT AGATTTGGAG GCAGAGTCAT GGAGTTTAGC TGTGGAGGCC
    CAGTTCTGTA GGATGCAAGA AAAACGCATC ATCAAGAGAC AGGATGTGAT TTACGAGTTC
    ATGCAGACCG AGCTCCATCA CGTGCAGACA CTCATGGTGA TGGCAGAGGT CTTCAGAAGG
    GGAATGCGGG ATGAAGTGGG TCTGGACGCA GAGACCATTG GCCGGATTTT TCCTTGCTTA
    GATGAGCTAC TCCAACTCCA TCGTGATTTT CTGACAGCAA TGAGGGAACG AAGGCAAAGC
    TGCGTCCAGA ACAACAGCAG CAAAAACTTT CTCATCCACC GTGTGGGTGA TATTTTTCTG
    CAACAGTTCT CCCAGGAGAA CGCAGAGAAG ATGAAGCAGG TGTATGGAGA GTTTTGCAGT
    CATCACACAG AAGCAGTCAG TTACTTCAAA GAGCTTCAGC AGCAGAACAA GAGATTTCAG
    CTCTTCATCA AACAACAGAG TAGTAACTCG CTGGTGAAGA GGCGAGAGAT CCCAGAATGT
    ATTTTGTTAG TGACGCAGCG AATTACAAAG TACCCTGTCC TCCTAGAGAG AATACTCCAG
    TACACAGACG AGGGCACAGA AGAGCACTCT GACCTCTCTC GAGCTCTAGT CCTGATCCGA
    GAGCTGATCT CCGCTGTGGA CCAATATGTG AGTCAGTATG AGCAGAACCA GAAGCTGAGT
    GAAGTCTTGG GCCGCATGGA GAACAAGTGC TCTACCAAAC TGAAGACCGG CTGCACTTTC
    CGCAAGCAGG ACATCACCAG CGGCAGGAAT CTGCTGCACC AGGGCCCGCT GCTCTGGAAG
    ACAGCCACTG GAAGACTGAA AGATGTTCTG GCATTTTTGC TGTCTGATTC ACTGGTGTTC
    CTTCAGGAGA AAGATCAGAA ATACATTTTT GCTACTGTGG ACCAGAAGCC TCCAGTGATC
    TCTTTGCAGA AACTAATTGT GCGTGAGGTG GCCAATGAAG AGAGAGGCAT GTTTCTGATC
    AGTGCATCTT CAGCCGGGCC TGAGATGTAC GAGGTGCATG CAGCCTCTAA AGATGAACGC
    AACGCCTGGA TGAGACTCAT CAGAGAGGCC GTGGAGAATT GCCCTGAAGA GGAGGATGAA
    ACTACAAGTG AGTCAGAAGA GGAAAGGAGA GTCACAGAAG CAAAGGTCCA AAAGATCCAC
    CGGCTGCAAG AGACGCTGTG TGGCCATGAT CACCTCATCT GTTCCAATCT GGAGGAGAAG
    CTGCACATCT ATGCCGAGCT GTCAGTGATG GCAGGCCAGC GAGAGGCAGG ACCAGAGCCC
    AGGCTGCTGG TACGACCCAG CACTGAAGAC GTTCCCCAGG CTGGAGTACT ACTCAGCGCC
    GCTCTCAGAG AGGCGGAGAG CCTAAGAGCA GCTCTCTCCT CTCATTGTGC TTCCACTCCT
    CCCGTCCCGT CCGGTCCAGA AGATCTAAAC AAACCTGAAC CCTGCACTCC TCCATCTCCT
    CTGCCTCCTG AAAGTCCACT GACAGACCCC ATCCAGAGTG AGGAGGAGGA GTGGAGAACT
    GAGGAAAGCT CAGAAGCACA GACCTTTAAT CACAACTCGG ACTCTGACCA CAATCTACAG
    CTGAAGGTGA GTCAGAGTGT GCAGAGCTTG ACTCAGCTGC TGTACAGTCT ACAGGCTGCA
    GTTACCATCC AAGACAGTTG CTATGAAGTG CAGAAGCTTC TTCTCCTGCT GATGTCAAGC
    CCCGCCCCTA ACCAACAGTC CCGCCCACTA TCCATCACCC GCAGCGGGCA GGCTCTGCAT
    GAGCAGGAGC GCCAGCGGGA GGCCGAGCGC CGCCGGGAAG AGCTGGCGGA TGTGGCTCGT
    CTGCGGGGAG CCCTGGTACG AGAGCGCCAG CAGTGGGAGA GGGAGTGCCA GGCGCGGCAG
    CTTCAGCACT CAGAACTGGA GACCACGCTT GAGCAGAGGG AGCTCTTGTG CCACCTAGAG
    GAGCGGAGGC TGCAGAGCGA GCGGCAGGAG CTCCAGGAGC AGCTGCAGGA ATACCAACAG
    AGTCTGGAGA GGCTCAGAGA AGGCCAGAGG AATGTGGAGA AGGAGAAGGA GAAACTGGAG
    ACTCAGAAGA TGCTGCTGGA GGGCTTGAAG CACGGGTGCC AGCAGAACCT TCCAGCTATG
    GTCATACCAC TGGACGGACA GCAGACACAG AATCACCTGG GCATTCAGGA CGAGCATGGC
    TCTATTTTTG TCAATGAGGC AGCGTTCATA TCTTCTTCTG TAAATAACAG GCACCTCCAT
    CTTCAGCATC ATCCACAGCA TCATCACCAG ACTGTCCATG TGCACTCAGG ATCTATAGGA
    TACTCAGATT CTCCCAGCGC TCAGAACAGT CTTAATTCAC TGCTAGCCCG ATCCAACCAC
    AGACCCAGCT CTGCTGGAGC ATCCGACCAC AGCTGGACGT CTCTGAGGAG TCTGACAGAG
    AGTGCAAACA ACAATGTGGG TCAGATCAGC CATGTTGAAG AGTCTTGGTC AGCAGGGGTA
    ACGGGAGCGG GACTCCATCA AACTGATGTC CTGCTCGTCA GCCAGCCCTT CCTAAGCATG
    GAGACAGAGA ACAGAGATAC TGATGGAGAG GAGACCATCG TGTATCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211758.1
    CDS197..5647
    Translation

    Target ORF information:

    RefSeq Version XM_017356269.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGTTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAACAGC CTCAAGAGAA AAGGGAGTTA AGGTTTCGCA GGCGAGCTCA GTCGGCTGAT
    GATGAAAGCA GTGCAGAGCT GGCCGACTCG CTCCAACATC TCACGCTCTC AGAGTTCCTC
    AAAGAGATTG AGGAAGAAGA ATGGGATAAA TATATCATCC CTTCCAAAAC AGAGTCAGAA
    AAGTACAAAG TCAGCCGCAC ATTCAGCTTC ATAAAGAGCC GAATGTATAG CACTCGCAAC
    AAGAACAAGG GGAAAGGAAA AGAGAAAGAG ACCAAAGACA AGCAGGTCAA CGGACACCAG
    TTCACAGCGG GATCTGTCCT GGGGTCGACA CTCTGTGAGA TCTGCAGCAA GCCAGCTTCA
    GGGAAAGAGG TTGTCCATTG CACACACTGT GCAGTGAGTG TCCATAAGGG CTGTAAGGAC
    TCATCTACGC CATGCCTGAA GAAACCTCAA GACAAATATT CAGTGATGAC AGTAAAAAGC
    AAGACAGCCT CTCTTCCTCA AAATTTTATT GTGCGTGAGA GTTCATCGTC GTCCCTTCAG
    ATCTCTACCT CTGCTTCTCT CCCTGTGATG ACATCACGAG ACTCGGCTCC TCTTCCTTGT
    CCTCTGTCCA GAAGTGTGCC CTTAACCACT GAAAGGCTTA GCGAGAGCCC AGAAGCTGAT
    AGTGATTCTA ATCTTTGGAG GAACCACATC CAATCAGAGG AGCTTCTCCA AACCATGGAG
    TCCTCAACAT CCACAGACTC GTCCATCATT GAAGACTCGG TTGATTCCCC TTTACAGATT
    GAGCTGCTGA TGAATGCTGT AGATTTGGAG GCAGAGTCAT GGAGTTTAGC TGTGGAGGCC
    CAGTTCTGTA GGATGCAAGA AAAACGCATC ATCAAGAGAC AGGATGTGAT TTACGAGTTC
    ATGCAGACCG AGCTCCATCA CGTGCAGACA CTCATGGTGA TGGCAGAGGT CTTCAGAAGG
    GGAATGCGGG ATGAAGTGGG TCTGGACGCA GAGACCATTG GCCGGATTTT TCCTTGCTTA
    GATGAGCTAC TCCAACTCCA TCGTGATTTT CTGACAGCAA TGAGGGAACG AAGGCAAAGC
    TGCGTCCAGA ACAACAGCAG CAAAAACTTT CTCATCCACC GTGTGGGTGA TATTTTTCTG
    CAACAGTTCT CCCAGGAGAA CGCAGAGAAG ATGAAGCAGG TGTATGGAGA GTTTTGCAGT
    CATCACACAG AAGCAGTCAG TTACTTCAAA GAGCTTCAGC AGCAGAACAA GAGATTTCAG
    CTCTTCATCA AACAACAGAG TAGTAACTCG CTGGTGAAGA GGCGAGAGAT CCCAGAATGT
    ATTTTGTTAG TGACGCAGCG AATTACAAAG TACCCTGTCC TCCTAGAGAG AATACTCCAG
    TACACAGACG AGGGCACAGA AGAGCACTCT GACCTCTCTC GAGCTCTAGT CCTGATCCGA
    GAGCTGATCT CCGCTGTGGA CCAATATGTG AGTCAGTATG AGCAGAACCA GAAGCTGAGT
    GAAGTCTTGG GCCGCATGGA GAACAAGTGC TCTACCAAAC TGAAGACCGG CTGCACTTTC
    CGCAAGCAGG ACATCACCAG CGGCAGGAAT CTGCTGCACC AGGGCCCGCT GCTCTGGAAG
    ACAGCCACTG GAAGACTGAA AGATGTTCTG GCATTTTTGC TGTCTGATTC ACTGGTGTTC
    CTTCAGGAGA AAGATCAGAA ATACATTTTT GCTACTGTGG ACCAGAAGCC TCCAGTGATC
    TCTTTGCAGA AACTAATTGT GCGTGAGGTG GCCAATGAAG AGAGAGGCAT GTTTCTGATC
    AGTGCATCTT CAGCCGGGCC TGAGATGTAC GAGGTGCATG CAGCCTCTAA AGATGAACGC
    AACGCCTGGA TGAGACTCAT CAGAGAGGCC GTGGAGAATT GCCCTGAAGA GGAGGATGAA
    ACTACAAGTG AGTCAGAAGA GGAAAGGAGA GTCACAGAAG CAAAGGTCCA AAAGATCCAC
    CGGCTGCAAG AGACGCTGTG TGGCCATGAT CACCTCATCT GTTCCAATCT GGAGGAGAAG
    CTGCACATCT ATGCCGAGCT GTCAGTGATG GCAGGCCAGC GAGAGGCAGG ACCAGAGCCC
    AGGCTGCTGG TACGACCCAG CACTGAAGAC GTTCCCCAGG CTGGAGTACT ACTCAGCGCC
    GCTCTCAGAG AGGCGGAGAG CCTAAGAGCA GCTCTCTCCT CTCATTGTGC TTCCACTCCT
    CCCGTCCCGT CCGGTCCAGA AGATCTAAAC AAACCTGAAC CCTGCACTCC TCCATCTCCT
    CTGCCTCCTG AAAGTCCACT GACAGACCCC ATCCAGAGTG AGGAGGAGGA GTGGAGAACT
    GAGGAAAGCT CAGAAGCACA GACCTTTAAT CACAACTCGG ACTCTGACCA CAATCTACAG
    CTGAAGGTGA GTCAGAGTGT GCAGAGCTTG ACTCAGCTGC TGTACAGTCT ACAGGCTGCA
    GTTACCATCC AAGACAGTTG CTATGAAGTG CAGAAGCTTC TTCTCCTGCT GATGTCAAGC
    CCCGCCCCTA ACCAACAGTC CCGCCCACTA TCCATCACCC GCAGCGGGCA GGCTCTGCAT
    GAGCAGGAGC GCCAGCGGGA GGCCGAGCGC CGCCGGGAAG AGCTGGCGGA TGTGGCTCGT
    CTGCGGGGAG CCCTGGTACG AGAGCGCCAG CAGTGGGAGA GGGAGTGCCA GGCGCGGCAG
    CTTCAGCACT CAGAACTGGA GACCACGCTT GAGCAGAGGG AGCTCTTGTG CCACCTAGAG
    GAGCGGAGGC TGCAGAGCGA GCGGCAGGAG CTCCAGGAGC AGCTGCAGGA ATACCAACAG
    AGTCTGGAGA GGCTCAGAGA AGGCCAGAGG AATGTGGAGA AGGAGAAGGA GAAACTGGAG
    ACTCAGAAGA TGCTGCTGGA GGGCTTGAAG CACGGGTGCC AGCAGAACCT TCCAGCTATG
    GTCATACCAC TGGACGGACA GCAGACACAG AATCACCTGG GCATTCAGGA CGAGCATGGC
    TCTATTTTTG TCAATGAGGC AGCGTTCATA TCTTCTTCTG TAAATAACAG GCACCTCCAT
    CTTCAGCATC ATCCACAGCA TCATCACCAG ACTGTCCATG TGCACTCAGG ATCTATAGGA
    TACTCAGATT CTCCCAGCGC TCAGAACAGT CTTAATTCAC TGCTAGCCCG ATCCAACCAC
    AGACCCAGCT CTGCTGGAGC ATCCGACCAC AGCTGGACGT CTCTGAGGAG TCTGACAGAG
    AGTGCAAACA ACAATGTGGG TCAGATCAGC CATGTTGAAG AGTCTTGGTC AGCAGGGGTA
    ACGGGAGCGG GACTCCATCA AACTGATGTC CTGCTCGTCA GCCAGCCCTT CCTAAGCATG
    GAGACAGAGA ACAGAGATAC TGATGGAGAG GAGACCATCG TGTATCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59220
    Clone ID Related Accession (Same CDS sequence) XM_017356270.1 , XM_021476532.1
    Accession Version XM_017356270.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAAC AGCCTCAAGA GAAAAGGGAG
    TTAAGGTTTC GCAGGCGAGC TCAGTCGGCT GATGATGAAA GCAGTGCAGA GCTGGCCGAC
    TCGCTCCAAC ATCTCACGCT CTCAGAGTTC CTCAAAGAGA TTGAGGAAGA AGAATGGGAT
    AAATATATCA TCCCTTCCAA AACAGAGTCA GAAAAGTACA AAGTCAGCCG CACATTCAGC
    TTCATAAAGA GCCGAATGTA TAGCACTCGC AACAAGAACA AGGGGAAAGG AAAAGAGAAA
    GAGACCAAAG ACAAGCAGGT CAACGGACAC CAGTTCACAG CGGGATCTGT CCTGGGGTCG
    ACACTCTGTG AGATCTGCAG CAAGCCAGCT TCAGGGAAAG AGGTTGTCCA TTGCACACAC
    TGTGCAGTGA GTGTCCATAA GGGCTGTAAG GACTCATCTA CGCCATGCCT GAAGAAACCT
    CAAGACAAAT ATTCAGTGAT GACAGTAAAA AGCAAGACAG CCTCTCTTCC TCAAAATTTT
    ATTGTGCGTG AGAGTTCATC GTCGTCCCTT CAGATCTCTA CCTCTGCTTC TCTCCCTGTG
    ATGACATCAC GAGACTCGGC TCCTCTTCCT TGTCCTCTGT CCAGAAGTGT GCCCTTAACC
    ACTGAAAGGC TTAGCGAGAG CCCAGAAGCT GATAGTGATT CTAATCTTTG GAGGAACCAC
    ATCCAATCAG AGGAGCTTCT CCAAACCATG GAGTCCTCAA CATCCACAGA CTCGTCCATC
    ATTGAAGACT CGGTTGATTC CCCTTTACAG ATTGAGCTGC TGATGAATGC TGTAGATTTG
    GAGGCAGAGT CATGGAGTTT AGCTGTGGAG GCCCAGTTCT GTAGGATGCA AGAAAAACGC
    ATCATCAAGA GACAGGATGT GATTTACGAG TTCATGCAGA CCGAGCTCCA TCACGTGCAG
    ACACTCATGG TGATGGCAGA GGTCTTCAGA AGGGGAATGC GGGATGAAGT GGGTCTGGAC
    GCAGAGACCA TTGGCCGGAT TTTTCCTTGC TTAGATGAGC TACTCCAACT CCATCGTGAT
    TTTCTGACAG CAATGAGGGA ACGAAGGCAA AGCTGCGTCC AGAACAACAG CAGCAAAAAC
    TTTCTCATCC ACCGTGTGGG TGATATTTTT CTGCAACAGT TCTCCCAGGA GAACGCAGAG
    AAGATGAAGC AGGTGTATGG AGAGTTTTGC AGTCATCACA CAGAAGCAGT CAGTTACTTC
    AAAGAGCTTC AGCAGCAGAA CAAGAGATTT CAGCTCTTCA TCAAACAACA GAGTAGTAAC
    TCGCTGGTGA AGAGGCGAGA GATCCCAGAA TGTATTTTGT TAGTGACGCA GCGAATTACA
    AAGTACCCTG TCCTCCTAGA GAGAATACTC CAGTACACAG ACGAGGGCAC AGAAGAGCAC
    TCTGACCTCT CTCGAGCTCT AGTCCTGATC CGAGAGCTGA TCTCCGCTGT GGACCAATAT
    GTGAGTCAGT ATGAGCAGAA CCAGAAGCTG AGTGAAGTCT TGGGCCGCAT GGAGAACAAG
    TGCTCTACCA AACTGAAGAC CGGCTGCACT TTCCGCAAGC AGGACATCAC CAGCGGCAGG
    AATCTGCTGC ACCAGGGCCC GCTGCTCTGG AAGACAGCCA CTGGAAGACT GAAAGATGTT
    CTGGCATTTT TGCTGTCTGA TTCACTGGTG TTCCTTCAGG AGAAAGATCA GAAATACATT
    TTTGCTACTG TGGACCAGAA GCCTCCAGTG ATCTCTTTGC AGAAACTAAT TGTGCGTGAG
    GTGGCCAATG AAGAGAGAGG CATGTTTCTG ATCAGTGCAT CTTCAGCCGG GCCTGAGATG
    TACGAGGTGC ATGCAGCCTC TAAAGATGAA CGCAACGCCT GGATGAGACT CATCAGAGAG
    GCCGTGGAGA ATTGCCCTGA AGAGGAGGAT GAAACTACAA GTGAGTCAGA AGAGGAAAGG
    AGAGTCACAG AAGCAAAGGT CCAAAAGATC CACCGGCTGC AAGAGACGCT GTGTGGCCAT
    GATCACCTCA TCTGTTCCAA TCTGGAGGAG AAGCTGCACA TCTATGCCGA GCTGTCAGTG
    ATGGCAGGCC AGCGAGAGGC AGGACCAGAG CCCAGGCTGC TGGTACGACC CAGCACTGAA
    GACGTTCCCC AGGCTGGAGT ACTACTCAGC GCCGCTCTCA GAGAGGCGGA GAGCCTAAGA
    GCAGCTCTCT CCTCTCATTG TGCTTCCACT CCTCCCGTCC CGTCCGGTCC AGAAGATCTA
    AACAAACCTG AACCCTGCAC TCCTCCATCT CCTCTGCCTC CTGAAAGTCC ACTGACAGAC
    CCCATCCAGA GTGAGGAGGA GGAGTGGAGA ACTGAGGAAA GCTCAGAAGC ACAGACCTTT
    AATCACAACT CGGACTCTGA CCACAATCTA CAGCTGAAGG TGAGTCAGAG TGTGCAGAGC
    TTGACTCAGC TGCTGTACAG TCTACAGGCT GCAGTTACCA TCCAAGACAG TTGCTATGAA
    GTGCAGAAGC TTCTTCTCCT GCTGATGTCA AGCCCCGCCC CTAACCAACA GTCCCGCCCA
    CTATCCATCA CCCGCAGCGG GCAGGCTCTG CATGAGCAGG AGCGCCAGCG GGAGGCCGAG
    CGCCGCCGGG AAGAGCTGGC GGATGTGGCT CGTCTGCGGG GAGCCCTGGT ACGAGAGCGC
    CAGCAGTGGG AGAGGGAGTG CCAGGCGCGG CAGCTTCAGC ACTCAGAACT GGAGACCACG
    CTTGAGCAGA GGGAGCTCTT GTGCCACCTA GAGGAGCGGA GGCTGCAGAG CGAGCGGCAG
    GAGCTCCAGG AGCAGCTGCA GGAATACCAA CAGAGTCTGG AGAGGCTCAG AGAAGGCCAG
    AGGAATGTGG AGAAGGAGAA GGAGAAACTG GAGACTCAGA AGATGCTGCT GGAGGGCTTG
    AAGCACGGGT GCCAGCAGAA CCTTCCAGCT ATGGTCATAC CACTGGACGG ACAGCAGACA
    CAGAATCACC TGGGCATTCA GGACGAGCAT GGCTCTATTT TTGTCAATGA GGCAGCGTTC
    ATATCTTCTT CTGTAAATAA CAGGCACCTC CATCTTCAGC ATCATCCACA GCATCATCAC
    CAGACTGTCC ATGTGCACTC AGGATCTATA GGATACTCAG ATTCTCCCAG CGCTCAGAAC
    AGTCTTAATT CACTGCTAGC CCGATCCAAC CACAGACCCA GCTCTGCTGG AGCATCCGAC
    CACAGCTGGA CGTCTCTGAG GAGTCTGACA GAGAGTGCAA ACAACAATGT GGGTCAGATC
    AGCCATGTTG AAGAGTCTTG GTCAGCAGGG GTAACGGGAG CGGGACTCCA TCAAACTGAT
    GTCCTGCTCG TCAGCCAGCC CTTCCTAAGC ATGGAGACAG AGAACAGAGA TACTGATGGA
    GAGGAGACCA TCGTGTATCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211759.1
    CDS197..5560
    Translation

    Target ORF information:

    RefSeq Version XM_017356270.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356270.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAAC AGCCTCAAGA GAAAAGGGAG
    TTAAGGTTTC GCAGGCGAGC TCAGTCGGCT GATGATGAAA GCAGTGCAGA GCTGGCCGAC
    TCGCTCCAAC ATCTCACGCT CTCAGAGTTC CTCAAAGAGA TTGAGGAAGA AGAATGGGAT
    AAATATATCA TCCCTTCCAA AACAGAGTCA GAAAAGTACA AAGTCAGCCG CACATTCAGC
    TTCATAAAGA GCCGAATGTA TAGCACTCGC AACAAGAACA AGGGGAAAGG AAAAGAGAAA
    GAGACCAAAG ACAAGCAGGT CAACGGACAC CAGTTCACAG CGGGATCTGT CCTGGGGTCG
    ACACTCTGTG AGATCTGCAG CAAGCCAGCT TCAGGGAAAG AGGTTGTCCA TTGCACACAC
    TGTGCAGTGA GTGTCCATAA GGGCTGTAAG GACTCATCTA CGCCATGCCT GAAGAAACCT
    CAAGACAAAT ATTCAGTGAT GACAGTAAAA AGCAAGACAG CCTCTCTTCC TCAAAATTTT
    ATTGTGCGTG AGAGTTCATC GTCGTCCCTT CAGATCTCTA CCTCTGCTTC TCTCCCTGTG
    ATGACATCAC GAGACTCGGC TCCTCTTCCT TGTCCTCTGT CCAGAAGTGT GCCCTTAACC
    ACTGAAAGGC TTAGCGAGAG CCCAGAAGCT GATAGTGATT CTAATCTTTG GAGGAACCAC
    ATCCAATCAG AGGAGCTTCT CCAAACCATG GAGTCCTCAA CATCCACAGA CTCGTCCATC
    ATTGAAGACT CGGTTGATTC CCCTTTACAG ATTGAGCTGC TGATGAATGC TGTAGATTTG
    GAGGCAGAGT CATGGAGTTT AGCTGTGGAG GCCCAGTTCT GTAGGATGCA AGAAAAACGC
    ATCATCAAGA GACAGGATGT GATTTACGAG TTCATGCAGA CCGAGCTCCA TCACGTGCAG
    ACACTCATGG TGATGGCAGA GGTCTTCAGA AGGGGAATGC GGGATGAAGT GGGTCTGGAC
    GCAGAGACCA TTGGCCGGAT TTTTCCTTGC TTAGATGAGC TACTCCAACT CCATCGTGAT
    TTTCTGACAG CAATGAGGGA ACGAAGGCAA AGCTGCGTCC AGAACAACAG CAGCAAAAAC
    TTTCTCATCC ACCGTGTGGG TGATATTTTT CTGCAACAGT TCTCCCAGGA GAACGCAGAG
    AAGATGAAGC AGGTGTATGG AGAGTTTTGC AGTCATCACA CAGAAGCAGT CAGTTACTTC
    AAAGAGCTTC AGCAGCAGAA CAAGAGATTT CAGCTCTTCA TCAAACAACA GAGTAGTAAC
    TCGCTGGTGA AGAGGCGAGA GATCCCAGAA TGTATTTTGT TAGTGACGCA GCGAATTACA
    AAGTACCCTG TCCTCCTAGA GAGAATACTC CAGTACACAG ACGAGGGCAC AGAAGAGCAC
    TCTGACCTCT CTCGAGCTCT AGTCCTGATC CGAGAGCTGA TCTCCGCTGT GGACCAATAT
    GTGAGTCAGT ATGAGCAGAA CCAGAAGCTG AGTGAAGTCT TGGGCCGCAT GGAGAACAAG
    TGCTCTACCA AACTGAAGAC CGGCTGCACT TTCCGCAAGC AGGACATCAC CAGCGGCAGG
    AATCTGCTGC ACCAGGGCCC GCTGCTCTGG AAGACAGCCA CTGGAAGACT GAAAGATGTT
    CTGGCATTTT TGCTGTCTGA TTCACTGGTG TTCCTTCAGG AGAAAGATCA GAAATACATT
    TTTGCTACTG TGGACCAGAA GCCTCCAGTG ATCTCTTTGC AGAAACTAAT TGTGCGTGAG
    GTGGCCAATG AAGAGAGAGG CATGTTTCTG ATCAGTGCAT CTTCAGCCGG GCCTGAGATG
    TACGAGGTGC ATGCAGCCTC TAAAGATGAA CGCAACGCCT GGATGAGACT CATCAGAGAG
    GCCGTGGAGA ATTGCCCTGA AGAGGAGGAT GAAACTACAA GTGAGTCAGA AGAGGAAAGG
    AGAGTCACAG AAGCAAAGGT CCAAAAGATC CACCGGCTGC AAGAGACGCT GTGTGGCCAT
    GATCACCTCA TCTGTTCCAA TCTGGAGGAG AAGCTGCACA TCTATGCCGA GCTGTCAGTG
    ATGGCAGGCC AGCGAGAGGC AGGACCAGAG CCCAGGCTGC TGGTACGACC CAGCACTGAA
    GACGTTCCCC AGGCTGGAGT ACTACTCAGC GCCGCTCTCA GAGAGGCGGA GAGCCTAAGA
    GCAGCTCTCT CCTCTCATTG TGCTTCCACT CCTCCCGTCC CGTCCGGTCC AGAAGATCTA
    AACAAACCTG AACCCTGCAC TCCTCCATCT CCTCTGCCTC CTGAAAGTCC ACTGACAGAC
    CCCATCCAGA GTGAGGAGGA GGAGTGGAGA ACTGAGGAAA GCTCAGAAGC ACAGACCTTT
    AATCACAACT CGGACTCTGA CCACAATCTA CAGCTGAAGG TGAGTCAGAG TGTGCAGAGC
    TTGACTCAGC TGCTGTACAG TCTACAGGCT GCAGTTACCA TCCAAGACAG TTGCTATGAA
    GTGCAGAAGC TTCTTCTCCT GCTGATGTCA AGCCCCGCCC CTAACCAACA GTCCCGCCCA
    CTATCCATCA CCCGCAGCGG GCAGGCTCTG CATGAGCAGG AGCGCCAGCG GGAGGCCGAG
    CGCCGCCGGG AAGAGCTGGC GGATGTGGCT CGTCTGCGGG GAGCCCTGGT ACGAGAGCGC
    CAGCAGTGGG AGAGGGAGTG CCAGGCGCGG CAGCTTCAGC ACTCAGAACT GGAGACCACG
    CTTGAGCAGA GGGAGCTCTT GTGCCACCTA GAGGAGCGGA GGCTGCAGAG CGAGCGGCAG
    GAGCTCCAGG AGCAGCTGCA GGAATACCAA CAGAGTCTGG AGAGGCTCAG AGAAGGCCAG
    AGGAATGTGG AGAAGGAGAA GGAGAAACTG GAGACTCAGA AGATGCTGCT GGAGGGCTTG
    AAGCACGGGT GCCAGCAGAA CCTTCCAGCT ATGGTCATAC CACTGGACGG ACAGCAGACA
    CAGAATCACC TGGGCATTCA GGACGAGCAT GGCTCTATTT TTGTCAATGA GGCAGCGTTC
    ATATCTTCTT CTGTAAATAA CAGGCACCTC CATCTTCAGC ATCATCCACA GCATCATCAC
    CAGACTGTCC ATGTGCACTC AGGATCTATA GGATACTCAG ATTCTCCCAG CGCTCAGAAC
    AGTCTTAATT CACTGCTAGC CCGATCCAAC CACAGACCCA GCTCTGCTGG AGCATCCGAC
    CACAGCTGGA CGTCTCTGAG GAGTCTGACA GAGAGTGCAA ACAACAATGT GGGTCAGATC
    AGCCATGTTG AAGAGTCTTG GTCAGCAGGG GTAACGGGAG CGGGACTCCA TCAAACTGAT
    GTCCTGCTCG TCAGCCAGCC CTTCCTAAGC ATGGAGACAG AGAACAGAGA TACTGATGGA
    GAGGAGACCA TCGTGTATCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59220
    Clone ID Related Accession (Same CDS sequence) XM_017356270.1 , XM_021476532.1
    Accession Version XM_021476532.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAAC AGCCTCAAGA GAAAAGGGAG
    TTAAGGTTTC GCAGGCGAGC TCAGTCGGCT GATGATGAAA GCAGTGCAGA GCTGGCCGAC
    TCGCTCCAAC ATCTCACGCT CTCAGAGTTC CTCAAAGAGA TTGAGGAAGA AGAATGGGAT
    AAATATATCA TCCCTTCCAA AACAGAGTCA GAAAAGTACA AAGTCAGCCG CACATTCAGC
    TTCATAAAGA GCCGAATGTA TAGCACTCGC AACAAGAACA AGGGGAAAGG AAAAGAGAAA
    GAGACCAAAG ACAAGCAGGT CAACGGACAC CAGTTCACAG CGGGATCTGT CCTGGGGTCG
    ACACTCTGTG AGATCTGCAG CAAGCCAGCT TCAGGGAAAG AGGTTGTCCA TTGCACACAC
    TGTGCAGTGA GTGTCCATAA GGGCTGTAAG GACTCATCTA CGCCATGCCT GAAGAAACCT
    CAAGACAAAT ATTCAGTGAT GACAGTAAAA AGCAAGACAG CCTCTCTTCC TCAAAATTTT
    ATTGTGCGTG AGAGTTCATC GTCGTCCCTT CAGATCTCTA CCTCTGCTTC TCTCCCTGTG
    ATGACATCAC GAGACTCGGC TCCTCTTCCT TGTCCTCTGT CCAGAAGTGT GCCCTTAACC
    ACTGAAAGGC TTAGCGAGAG CCCAGAAGCT GATAGTGATT CTAATCTTTG GAGGAACCAC
    ATCCAATCAG AGGAGCTTCT CCAAACCATG GAGTCCTCAA CATCCACAGA CTCGTCCATC
    ATTGAAGACT CGGTTGATTC CCCTTTACAG ATTGAGCTGC TGATGAATGC TGTAGATTTG
    GAGGCAGAGT CATGGAGTTT AGCTGTGGAG GCCCAGTTCT GTAGGATGCA AGAAAAACGC
    ATCATCAAGA GACAGGATGT GATTTACGAG TTCATGCAGA CCGAGCTCCA TCACGTGCAG
    ACACTCATGG TGATGGCAGA GGTCTTCAGA AGGGGAATGC GGGATGAAGT GGGTCTGGAC
    GCAGAGACCA TTGGCCGGAT TTTTCCTTGC TTAGATGAGC TACTCCAACT CCATCGTGAT
    TTTCTGACAG CAATGAGGGA ACGAAGGCAA AGCTGCGTCC AGAACAACAG CAGCAAAAAC
    TTTCTCATCC ACCGTGTGGG TGATATTTTT CTGCAACAGT TCTCCCAGGA GAACGCAGAG
    AAGATGAAGC AGGTGTATGG AGAGTTTTGC AGTCATCACA CAGAAGCAGT CAGTTACTTC
    AAAGAGCTTC AGCAGCAGAA CAAGAGATTT CAGCTCTTCA TCAAACAACA GAGTAGTAAC
    TCGCTGGTGA AGAGGCGAGA GATCCCAGAA TGTATTTTGT TAGTGACGCA GCGAATTACA
    AAGTACCCTG TCCTCCTAGA GAGAATACTC CAGTACACAG ACGAGGGCAC AGAAGAGCAC
    TCTGACCTCT CTCGAGCTCT AGTCCTGATC CGAGAGCTGA TCTCCGCTGT GGACCAATAT
    GTGAGTCAGT ATGAGCAGAA CCAGAAGCTG AGTGAAGTCT TGGGCCGCAT GGAGAACAAG
    TGCTCTACCA AACTGAAGAC CGGCTGCACT TTCCGCAAGC AGGACATCAC CAGCGGCAGG
    AATCTGCTGC ACCAGGGCCC GCTGCTCTGG AAGACAGCCA CTGGAAGACT GAAAGATGTT
    CTGGCATTTT TGCTGTCTGA TTCACTGGTG TTCCTTCAGG AGAAAGATCA GAAATACATT
    TTTGCTACTG TGGACCAGAA GCCTCCAGTG ATCTCTTTGC AGAAACTAAT TGTGCGTGAG
    GTGGCCAATG AAGAGAGAGG CATGTTTCTG ATCAGTGCAT CTTCAGCCGG GCCTGAGATG
    TACGAGGTGC ATGCAGCCTC TAAAGATGAA CGCAACGCCT GGATGAGACT CATCAGAGAG
    GCCGTGGAGA ATTGCCCTGA AGAGGAGGAT GAAACTACAA GTGAGTCAGA AGAGGAAAGG
    AGAGTCACAG AAGCAAAGGT CCAAAAGATC CACCGGCTGC AAGAGACGCT GTGTGGCCAT
    GATCACCTCA TCTGTTCCAA TCTGGAGGAG AAGCTGCACA TCTATGCCGA GCTGTCAGTG
    ATGGCAGGCC AGCGAGAGGC AGGACCAGAG CCCAGGCTGC TGGTACGACC CAGCACTGAA
    GACGTTCCCC AGGCTGGAGT ACTACTCAGC GCCGCTCTCA GAGAGGCGGA GAGCCTAAGA
    GCAGCTCTCT CCTCTCATTG TGCTTCCACT CCTCCCGTCC CGTCCGGTCC AGAAGATCTA
    AACAAACCTG AACCCTGCAC TCCTCCATCT CCTCTGCCTC CTGAAAGTCC ACTGACAGAC
    CCCATCCAGA GTGAGGAGGA GGAGTGGAGA ACTGAGGAAA GCTCAGAAGC ACAGACCTTT
    AATCACAACT CGGACTCTGA CCACAATCTA CAGCTGAAGG TGAGTCAGAG TGTGCAGAGC
    TTGACTCAGC TGCTGTACAG TCTACAGGCT GCAGTTACCA TCCAAGACAG TTGCTATGAA
    GTGCAGAAGC TTCTTCTCCT GCTGATGTCA AGCCCCGCCC CTAACCAACA GTCCCGCCCA
    CTATCCATCA CCCGCAGCGG GCAGGCTCTG CATGAGCAGG AGCGCCAGCG GGAGGCCGAG
    CGCCGCCGGG AAGAGCTGGC GGATGTGGCT CGTCTGCGGG GAGCCCTGGT ACGAGAGCGC
    CAGCAGTGGG AGAGGGAGTG CCAGGCGCGG CAGCTTCAGC ACTCAGAACT GGAGACCACG
    CTTGAGCAGA GGGAGCTCTT GTGCCACCTA GAGGAGCGGA GGCTGCAGAG CGAGCGGCAG
    GAGCTCCAGG AGCAGCTGCA GGAATACCAA CAGAGTCTGG AGAGGCTCAG AGAAGGCCAG
    AGGAATGTGG AGAAGGAGAA GGAGAAACTG GAGACTCAGA AGATGCTGCT GGAGGGCTTG
    AAGCACGGGT GCCAGCAGAA CCTTCCAGCT ATGGTCATAC CACTGGACGG ACAGCAGACA
    CAGAATCACC TGGGCATTCA GGACGAGCAT GGCTCTATTT TTGTCAATGA GGCAGCGTTC
    ATATCTTCTT CTGTAAATAA CAGGCACCTC CATCTTCAGC ATCATCCACA GCATCATCAC
    CAGACTGTCC ATGTGCACTC AGGATCTATA GGATACTCAG ATTCTCCCAG CGCTCAGAAC
    AGTCTTAATT CACTGCTAGC CCGATCCAAC CACAGACCCA GCTCTGCTGG AGCATCCGAC
    CACAGCTGGA CGTCTCTGAG GAGTCTGACA GAGAGTGCAA ACAACAATGT GGGTCAGATC
    AGCCATGTTG AAGAGTCTTG GTCAGCAGGG GTAACGGGAG CGGGACTCCA TCAAACTGAT
    GTCCTGCTCG TCAGCCAGCC CTTCCTAAGC ATGGAGACAG AGAACAGAGA TACTGATGGA
    GAGGAGACCA TCGTGTATCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332207.1
    CDS206..5569
    Translation

    Target ORF information:

    RefSeq Version XM_021476532.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAAC AGCCTCAAGA GAAAAGGGAG
    TTAAGGTTTC GCAGGCGAGC TCAGTCGGCT GATGATGAAA GCAGTGCAGA GCTGGCCGAC
    TCGCTCCAAC ATCTCACGCT CTCAGAGTTC CTCAAAGAGA TTGAGGAAGA AGAATGGGAT
    AAATATATCA TCCCTTCCAA AACAGAGTCA GAAAAGTACA AAGTCAGCCG CACATTCAGC
    TTCATAAAGA GCCGAATGTA TAGCACTCGC AACAAGAACA AGGGGAAAGG AAAAGAGAAA
    GAGACCAAAG ACAAGCAGGT CAACGGACAC CAGTTCACAG CGGGATCTGT CCTGGGGTCG
    ACACTCTGTG AGATCTGCAG CAAGCCAGCT TCAGGGAAAG AGGTTGTCCA TTGCACACAC
    TGTGCAGTGA GTGTCCATAA GGGCTGTAAG GACTCATCTA CGCCATGCCT GAAGAAACCT
    CAAGACAAAT ATTCAGTGAT GACAGTAAAA AGCAAGACAG CCTCTCTTCC TCAAAATTTT
    ATTGTGCGTG AGAGTTCATC GTCGTCCCTT CAGATCTCTA CCTCTGCTTC TCTCCCTGTG
    ATGACATCAC GAGACTCGGC TCCTCTTCCT TGTCCTCTGT CCAGAAGTGT GCCCTTAACC
    ACTGAAAGGC TTAGCGAGAG CCCAGAAGCT GATAGTGATT CTAATCTTTG GAGGAACCAC
    ATCCAATCAG AGGAGCTTCT CCAAACCATG GAGTCCTCAA CATCCACAGA CTCGTCCATC
    ATTGAAGACT CGGTTGATTC CCCTTTACAG ATTGAGCTGC TGATGAATGC TGTAGATTTG
    GAGGCAGAGT CATGGAGTTT AGCTGTGGAG GCCCAGTTCT GTAGGATGCA AGAAAAACGC
    ATCATCAAGA GACAGGATGT GATTTACGAG TTCATGCAGA CCGAGCTCCA TCACGTGCAG
    ACACTCATGG TGATGGCAGA GGTCTTCAGA AGGGGAATGC GGGATGAAGT GGGTCTGGAC
    GCAGAGACCA TTGGCCGGAT TTTTCCTTGC TTAGATGAGC TACTCCAACT CCATCGTGAT
    TTTCTGACAG CAATGAGGGA ACGAAGGCAA AGCTGCGTCC AGAACAACAG CAGCAAAAAC
    TTTCTCATCC ACCGTGTGGG TGATATTTTT CTGCAACAGT TCTCCCAGGA GAACGCAGAG
    AAGATGAAGC AGGTGTATGG AGAGTTTTGC AGTCATCACA CAGAAGCAGT CAGTTACTTC
    AAAGAGCTTC AGCAGCAGAA CAAGAGATTT CAGCTCTTCA TCAAACAACA GAGTAGTAAC
    TCGCTGGTGA AGAGGCGAGA GATCCCAGAA TGTATTTTGT TAGTGACGCA GCGAATTACA
    AAGTACCCTG TCCTCCTAGA GAGAATACTC CAGTACACAG ACGAGGGCAC AGAAGAGCAC
    TCTGACCTCT CTCGAGCTCT AGTCCTGATC CGAGAGCTGA TCTCCGCTGT GGACCAATAT
    GTGAGTCAGT ATGAGCAGAA CCAGAAGCTG AGTGAAGTCT TGGGCCGCAT GGAGAACAAG
    TGCTCTACCA AACTGAAGAC CGGCTGCACT TTCCGCAAGC AGGACATCAC CAGCGGCAGG
    AATCTGCTGC ACCAGGGCCC GCTGCTCTGG AAGACAGCCA CTGGAAGACT GAAAGATGTT
    CTGGCATTTT TGCTGTCTGA TTCACTGGTG TTCCTTCAGG AGAAAGATCA GAAATACATT
    TTTGCTACTG TGGACCAGAA GCCTCCAGTG ATCTCTTTGC AGAAACTAAT TGTGCGTGAG
    GTGGCCAATG AAGAGAGAGG CATGTTTCTG ATCAGTGCAT CTTCAGCCGG GCCTGAGATG
    TACGAGGTGC ATGCAGCCTC TAAAGATGAA CGCAACGCCT GGATGAGACT CATCAGAGAG
    GCCGTGGAGA ATTGCCCTGA AGAGGAGGAT GAAACTACAA GTGAGTCAGA AGAGGAAAGG
    AGAGTCACAG AAGCAAAGGT CCAAAAGATC CACCGGCTGC AAGAGACGCT GTGTGGCCAT
    GATCACCTCA TCTGTTCCAA TCTGGAGGAG AAGCTGCACA TCTATGCCGA GCTGTCAGTG
    ATGGCAGGCC AGCGAGAGGC AGGACCAGAG CCCAGGCTGC TGGTACGACC CAGCACTGAA
    GACGTTCCCC AGGCTGGAGT ACTACTCAGC GCCGCTCTCA GAGAGGCGGA GAGCCTAAGA
    GCAGCTCTCT CCTCTCATTG TGCTTCCACT CCTCCCGTCC CGTCCGGTCC AGAAGATCTA
    AACAAACCTG AACCCTGCAC TCCTCCATCT CCTCTGCCTC CTGAAAGTCC ACTGACAGAC
    CCCATCCAGA GTGAGGAGGA GGAGTGGAGA ACTGAGGAAA GCTCAGAAGC ACAGACCTTT
    AATCACAACT CGGACTCTGA CCACAATCTA CAGCTGAAGG TGAGTCAGAG TGTGCAGAGC
    TTGACTCAGC TGCTGTACAG TCTACAGGCT GCAGTTACCA TCCAAGACAG TTGCTATGAA
    GTGCAGAAGC TTCTTCTCCT GCTGATGTCA AGCCCCGCCC CTAACCAACA GTCCCGCCCA
    CTATCCATCA CCCGCAGCGG GCAGGCTCTG CATGAGCAGG AGCGCCAGCG GGAGGCCGAG
    CGCCGCCGGG AAGAGCTGGC GGATGTGGCT CGTCTGCGGG GAGCCCTGGT ACGAGAGCGC
    CAGCAGTGGG AGAGGGAGTG CCAGGCGCGG CAGCTTCAGC ACTCAGAACT GGAGACCACG
    CTTGAGCAGA GGGAGCTCTT GTGCCACCTA GAGGAGCGGA GGCTGCAGAG CGAGCGGCAG
    GAGCTCCAGG AGCAGCTGCA GGAATACCAA CAGAGTCTGG AGAGGCTCAG AGAAGGCCAG
    AGGAATGTGG AGAAGGAGAA GGAGAAACTG GAGACTCAGA AGATGCTGCT GGAGGGCTTG
    AAGCACGGGT GCCAGCAGAA CCTTCCAGCT ATGGTCATAC CACTGGACGG ACAGCAGACA
    CAGAATCACC TGGGCATTCA GGACGAGCAT GGCTCTATTT TTGTCAATGA GGCAGCGTTC
    ATATCTTCTT CTGTAAATAA CAGGCACCTC CATCTTCAGC ATCATCCACA GCATCATCAC
    CAGACTGTCC ATGTGCACTC AGGATCTATA GGATACTCAG ATTCTCCCAG CGCTCAGAAC
    AGTCTTAATT CACTGCTAGC CCGATCCAAC CACAGACCCA GCTCTGCTGG AGCATCCGAC
    CACAGCTGGA CGTCTCTGAG GAGTCTGACA GAGAGTGCAA ACAACAATGT GGGTCAGATC
    AGCCATGTTG AAGAGTCTTG GTCAGCAGGG GTAACGGGAG CGGGACTCCA TCAAACTGAT
    GTCCTGCTCG TCAGCCAGCC CTTCCTAAGC ATGGAGACAG AGAACAGAGA TACTGATGGA
    GAGGAGACCA TCGTGTATCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59221
    Clone ID Related Accession (Same CDS sequence) XM_017356271.1 , XM_021476533.1
    Accession Version XM_017356271.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGTTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAACAGC CTCAAGAGAA AAGGATTGAG GAAGAAGAAT GGGATAAATA TATCATCCCT
    TCCAAAACAG AGTCAGAAAA GTACAAAGTC AGCCGCACAT TCAGCTTCAT AAAGAGCCGA
    ATGTATAGCA CTCGCAACAA GAACAAGGGG AAAGGAAAAG AGAAAGAGAC CAAAGACAAG
    CAGGTCAACG GACACCAGTT CACAGCGGGA TCTGTCCTGG GGTCGACACT CTGTGAGATC
    TGCAGCAAGC CAGCTTCAGG GAAAGAGGTT GTCCATTGCA CACACTGTGC AGTGAGTGTC
    CATAAGGGCT GTAAGGACTC ATCTACGCCA TGCCTGAAGA AACCTCAAGA CAAATATTCA
    GTGATGACAG TAAAAAGCAA GACAGCCTCT CTTCCTCAAA ATTTTATTGT GCGTGAGAGT
    TCATCGTCGT CCCTTCAGAT CTCTACCTCT GCTTCTCTCC CTGTGATGAC ATCACGAGAC
    TCGGCTCCTC TTCCTTGTCC TCTGTCCAGA AGTGTGCCCT TAACCACTGA AAGGCTTAGC
    GAGAGCCCAG AAGCTGATAG TGATTCTAAT CTTTGGAGGA ACCACATCCA ATCAGAGGAG
    CTTCTCCAAA CCATGGAGTC CTCAACATCC ACAGACTCGT CCATCATTGA AGACTCGGTT
    GATTCCCCTT TACAGATTGA GCTGCTGATG AATGCTGTAG ATTTGGAGGC AGAGTCATGG
    AGTTTAGCTG TGGAGGCCCA GTTCTGTAGG ATGCAAGAAA AACGCATCAT CAAGAGACAG
    GATGTGATTT ACGAGTTCAT GCAGACCGAG CTCCATCACG TGCAGACACT CATGGTGATG
    GCAGAGGTCT TCAGAAGGGG AATGCGGGAT GAAGTGGGTC TGGACGCAGA GACCATTGGC
    CGGATTTTTC CTTGCTTAGA TGAGCTACTC CAACTCCATC GTGATTTTCT GACAGCAATG
    AGGGAACGAA GGCAAAGCTG CGTCCAGAAC AACAGCAGCA AAAACTTTCT CATCCACCGT
    GTGGGTGATA TTTTTCTGCA ACAGTTCTCC CAGGAGAACG CAGAGAAGAT GAAGCAGGTG
    TATGGAGAGT TTTGCAGTCA TCACACAGAA GCAGTCAGTT ACTTCAAAGA GCTTCAGCAG
    CAGAACAAGA GATTTCAGCT CTTCATCAAA CAACAGAGTA GTAACTCGCT GGTGAAGAGG
    CGAGAGATCC CAGAATGTAT TTTGTTAGTG ACGCAGCGAA TTACAAAGTA CCCTGTCCTC
    CTAGAGAGAA TACTCCAGTA CACAGACGAG GGCACAGAAG AGCACTCTGA CCTCTCTCGA
    GCTCTAGTCC TGATCCGAGA GCTGATCTCC GCTGTGGACC AATATGTGAG TCAGTATGAG
    CAGAACCAGA AGCTGAGTGA AGTCTTGGGC CGCATGGAGA ACAAGTGCTC TACCAAACTG
    AAGACCGGCT GCACTTTCCG CAAGCAGGAC ATCACCAGCG GCAGGAATCT GCTGCACCAG
    GGCCCGCTGC TCTGGAAGAC AGCCACTGGA AGACTGAAAG ATGTTCTGGC ATTTTTGCTG
    TCTGATTCAC TGGTGTTCCT TCAGGAGAAA GATCAGAAAT ACATTTTTGC TACTGTGGAC
    CAGAAGCCTC CAGTGATCTC TTTGCAGAAA CTAATTGTGC GTGAGGTGGC CAATGAAGAG
    AGAGGCATGT TTCTGATCAG TGCATCTTCA GCCGGGCCTG AGATGTACGA GGTGCATGCA
    GCCTCTAAAG ATGAACGCAA CGCCTGGATG AGACTCATCA GAGAGGCCGT GGAGAATTGC
    CCTGAAGAGG AGGATGAAAC TACAAGTGAG TCAGAAGAGG AAAGGAGAGT CACAGAAGCA
    AAGGTCCAAA AGATCCACCG GCTGCAAGAG ACGCTGTGTG GCCATGATCA CCTCATCTGT
    TCCAATCTGG AGGAGAAGCT GCACATCTAT GCCGAGCTGT CAGTGATGGC AGGCCAGCGA
    GAGGCAGGAC CAGAGCCCAG GCTGCTGGTA CGACCCAGCA CTGAAGACGT TCCCCAGGCT
    GGAGTACTAC TCAGCGCCGC TCTCAGAGAG GCGGAGAGCC TAAGAGCAGC TCTCTCCTCT
    CATTGTGCTT CCACTCCTCC CGTCCCGTCC GGTCCAGAAG ATCTAAACAA ACCTGAACCC
    TGCACTCCTC CATCTCCTCT GCCTCCTGAA AGTCCACTGA CAGACCCCAT CCAGAGTGAG
    GAGGAGGAGT GGAGAACTGA GGAAAGCTCA GAAGCACAGA CCTTTAATCA CAACTCGGAC
    TCTGACCACA ATCTACAGCT GAAGGTGAGT CAGAGTGTGC AGAGCTTGAC TCAGCTGCTG
    TACAGTCTAC AGGCTGCAGT TACCATCCAA GACAGTTGCT ATGAAGTGCA GAAGCTTCTT
    CTCCTGCTGA TGTCAAGCCC CGCCCCTAAC CAACAGTCCC GCCCACTATC CATCACCCGC
    AGCGGGCAGG CTCTGCATGA GCAGGAGCGC CAGCGGGAGG CCGAGCGCCG CCGGGAAGAG
    CTGGCGGATG TGGCTCGTCT GCGGGGAGCC CTGGTACGAG AGCGCCAGCA GTGGGAGAGG
    GAGTGCCAGG CGCGGCAGCT TCAGCACTCA GAACTGGAGA CCACGCTTGA GCAGAGGGAG
    CTCTTGTGCC ACCTAGAGGA GCGGAGGCTG CAGAGCGAGC GGCAGGAGCT CCAGGAGCAG
    CTGCAGGAAT ACCAACAGAG TCTGGAGAGG CTCAGAGAAG GCCAGAGGAA TGTGGAGAAG
    GAGAAGGAGA AACTGGAGAC TCAGAAGATG CTGCTGGAGG GCTTGAAGCA CGGGTGCCAG
    CAGAACCTTC CAGCTATGGT CATACCACTG GACGGACAGC AGACACAGAA TCACCTGGGC
    ATTCAGGACG AGCATGGCTC TATTTTTGTC AATGAGGCAG CGTTCATATC TTCTTCTGTA
    AATAACAGGC ACCTCCATCT TCAGCATCAT CCACAGCATC ATCACCAGAC TGTCCATGTG
    CACTCAGGAT CTATAGGATA CTCAGATTCT CCCAGCGCTC AGAACAGTCT TAATTCACTG
    CTAGCCCGAT CCAACCACAG ACCCAGCTCT GCTGGAGCAT CCGACCACAG CTGGACGTCT
    CTGAGGAGTC TGACAGAGAG TGCAAACAAC AATGTGGGTC AGATCAGCCA TGTTGAAGAG
    TCTTGGTCAG CAGGGGTAAC GGGAGCGGGA CTCCATCAAA CTGATGTCCT GCTCGTCAGC
    CAGCCCTTCC TAAGCATGGA GACAGAGAAC AGAGATACTG ATGGAGAGGA GACCATCGTG
    TATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211760.1
    CDS197..5545
    Translation

    Target ORF information:

    RefSeq Version XM_017356271.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356271.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGTTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAACAGC CTCAAGAGAA AAGGATTGAG GAAGAAGAAT GGGATAAATA TATCATCCCT
    TCCAAAACAG AGTCAGAAAA GTACAAAGTC AGCCGCACAT TCAGCTTCAT AAAGAGCCGA
    ATGTATAGCA CTCGCAACAA GAACAAGGGG AAAGGAAAAG AGAAAGAGAC CAAAGACAAG
    CAGGTCAACG GACACCAGTT CACAGCGGGA TCTGTCCTGG GGTCGACACT CTGTGAGATC
    TGCAGCAAGC CAGCTTCAGG GAAAGAGGTT GTCCATTGCA CACACTGTGC AGTGAGTGTC
    CATAAGGGCT GTAAGGACTC ATCTACGCCA TGCCTGAAGA AACCTCAAGA CAAATATTCA
    GTGATGACAG TAAAAAGCAA GACAGCCTCT CTTCCTCAAA ATTTTATTGT GCGTGAGAGT
    TCATCGTCGT CCCTTCAGAT CTCTACCTCT GCTTCTCTCC CTGTGATGAC ATCACGAGAC
    TCGGCTCCTC TTCCTTGTCC TCTGTCCAGA AGTGTGCCCT TAACCACTGA AAGGCTTAGC
    GAGAGCCCAG AAGCTGATAG TGATTCTAAT CTTTGGAGGA ACCACATCCA ATCAGAGGAG
    CTTCTCCAAA CCATGGAGTC CTCAACATCC ACAGACTCGT CCATCATTGA AGACTCGGTT
    GATTCCCCTT TACAGATTGA GCTGCTGATG AATGCTGTAG ATTTGGAGGC AGAGTCATGG
    AGTTTAGCTG TGGAGGCCCA GTTCTGTAGG ATGCAAGAAA AACGCATCAT CAAGAGACAG
    GATGTGATTT ACGAGTTCAT GCAGACCGAG CTCCATCACG TGCAGACACT CATGGTGATG
    GCAGAGGTCT TCAGAAGGGG AATGCGGGAT GAAGTGGGTC TGGACGCAGA GACCATTGGC
    CGGATTTTTC CTTGCTTAGA TGAGCTACTC CAACTCCATC GTGATTTTCT GACAGCAATG
    AGGGAACGAA GGCAAAGCTG CGTCCAGAAC AACAGCAGCA AAAACTTTCT CATCCACCGT
    GTGGGTGATA TTTTTCTGCA ACAGTTCTCC CAGGAGAACG CAGAGAAGAT GAAGCAGGTG
    TATGGAGAGT TTTGCAGTCA TCACACAGAA GCAGTCAGTT ACTTCAAAGA GCTTCAGCAG
    CAGAACAAGA GATTTCAGCT CTTCATCAAA CAACAGAGTA GTAACTCGCT GGTGAAGAGG
    CGAGAGATCC CAGAATGTAT TTTGTTAGTG ACGCAGCGAA TTACAAAGTA CCCTGTCCTC
    CTAGAGAGAA TACTCCAGTA CACAGACGAG GGCACAGAAG AGCACTCTGA CCTCTCTCGA
    GCTCTAGTCC TGATCCGAGA GCTGATCTCC GCTGTGGACC AATATGTGAG TCAGTATGAG
    CAGAACCAGA AGCTGAGTGA AGTCTTGGGC CGCATGGAGA ACAAGTGCTC TACCAAACTG
    AAGACCGGCT GCACTTTCCG CAAGCAGGAC ATCACCAGCG GCAGGAATCT GCTGCACCAG
    GGCCCGCTGC TCTGGAAGAC AGCCACTGGA AGACTGAAAG ATGTTCTGGC ATTTTTGCTG
    TCTGATTCAC TGGTGTTCCT TCAGGAGAAA GATCAGAAAT ACATTTTTGC TACTGTGGAC
    CAGAAGCCTC CAGTGATCTC TTTGCAGAAA CTAATTGTGC GTGAGGTGGC CAATGAAGAG
    AGAGGCATGT TTCTGATCAG TGCATCTTCA GCCGGGCCTG AGATGTACGA GGTGCATGCA
    GCCTCTAAAG ATGAACGCAA CGCCTGGATG AGACTCATCA GAGAGGCCGT GGAGAATTGC
    CCTGAAGAGG AGGATGAAAC TACAAGTGAG TCAGAAGAGG AAAGGAGAGT CACAGAAGCA
    AAGGTCCAAA AGATCCACCG GCTGCAAGAG ACGCTGTGTG GCCATGATCA CCTCATCTGT
    TCCAATCTGG AGGAGAAGCT GCACATCTAT GCCGAGCTGT CAGTGATGGC AGGCCAGCGA
    GAGGCAGGAC CAGAGCCCAG GCTGCTGGTA CGACCCAGCA CTGAAGACGT TCCCCAGGCT
    GGAGTACTAC TCAGCGCCGC TCTCAGAGAG GCGGAGAGCC TAAGAGCAGC TCTCTCCTCT
    CATTGTGCTT CCACTCCTCC CGTCCCGTCC GGTCCAGAAG ATCTAAACAA ACCTGAACCC
    TGCACTCCTC CATCTCCTCT GCCTCCTGAA AGTCCACTGA CAGACCCCAT CCAGAGTGAG
    GAGGAGGAGT GGAGAACTGA GGAAAGCTCA GAAGCACAGA CCTTTAATCA CAACTCGGAC
    TCTGACCACA ATCTACAGCT GAAGGTGAGT CAGAGTGTGC AGAGCTTGAC TCAGCTGCTG
    TACAGTCTAC AGGCTGCAGT TACCATCCAA GACAGTTGCT ATGAAGTGCA GAAGCTTCTT
    CTCCTGCTGA TGTCAAGCCC CGCCCCTAAC CAACAGTCCC GCCCACTATC CATCACCCGC
    AGCGGGCAGG CTCTGCATGA GCAGGAGCGC CAGCGGGAGG CCGAGCGCCG CCGGGAAGAG
    CTGGCGGATG TGGCTCGTCT GCGGGGAGCC CTGGTACGAG AGCGCCAGCA GTGGGAGAGG
    GAGTGCCAGG CGCGGCAGCT TCAGCACTCA GAACTGGAGA CCACGCTTGA GCAGAGGGAG
    CTCTTGTGCC ACCTAGAGGA GCGGAGGCTG CAGAGCGAGC GGCAGGAGCT CCAGGAGCAG
    CTGCAGGAAT ACCAACAGAG TCTGGAGAGG CTCAGAGAAG GCCAGAGGAA TGTGGAGAAG
    GAGAAGGAGA AACTGGAGAC TCAGAAGATG CTGCTGGAGG GCTTGAAGCA CGGGTGCCAG
    CAGAACCTTC CAGCTATGGT CATACCACTG GACGGACAGC AGACACAGAA TCACCTGGGC
    ATTCAGGACG AGCATGGCTC TATTTTTGTC AATGAGGCAG CGTTCATATC TTCTTCTGTA
    AATAACAGGC ACCTCCATCT TCAGCATCAT CCACAGCATC ATCACCAGAC TGTCCATGTG
    CACTCAGGAT CTATAGGATA CTCAGATTCT CCCAGCGCTC AGAACAGTCT TAATTCACTG
    CTAGCCCGAT CCAACCACAG ACCCAGCTCT GCTGGAGCAT CCGACCACAG CTGGACGTCT
    CTGAGGAGTC TGACAGAGAG TGCAAACAAC AATGTGGGTC AGATCAGCCA TGTTGAAGAG
    TCTTGGTCAG CAGGGGTAAC GGGAGCGGGA CTCCATCAAA CTGATGTCCT GCTCGTCAGC
    CAGCCCTTCC TAAGCATGGA GACAGAGAAC AGAGATACTG ATGGAGAGGA GACCATCGTG
    TATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59221
    Clone ID Related Accession (Same CDS sequence) XM_017356271.1 , XM_021476533.1
    Accession Version XM_021476533.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGTTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAACAGC CTCAAGAGAA AAGGATTGAG GAAGAAGAAT GGGATAAATA TATCATCCCT
    TCCAAAACAG AGTCAGAAAA GTACAAAGTC AGCCGCACAT TCAGCTTCAT AAAGAGCCGA
    ATGTATAGCA CTCGCAACAA GAACAAGGGG AAAGGAAAAG AGAAAGAGAC CAAAGACAAG
    CAGGTCAACG GACACCAGTT CACAGCGGGA TCTGTCCTGG GGTCGACACT CTGTGAGATC
    TGCAGCAAGC CAGCTTCAGG GAAAGAGGTT GTCCATTGCA CACACTGTGC AGTGAGTGTC
    CATAAGGGCT GTAAGGACTC ATCTACGCCA TGCCTGAAGA AACCTCAAGA CAAATATTCA
    GTGATGACAG TAAAAAGCAA GACAGCCTCT CTTCCTCAAA ATTTTATTGT GCGTGAGAGT
    TCATCGTCGT CCCTTCAGAT CTCTACCTCT GCTTCTCTCC CTGTGATGAC ATCACGAGAC
    TCGGCTCCTC TTCCTTGTCC TCTGTCCAGA AGTGTGCCCT TAACCACTGA AAGGCTTAGC
    GAGAGCCCAG AAGCTGATAG TGATTCTAAT CTTTGGAGGA ACCACATCCA ATCAGAGGAG
    CTTCTCCAAA CCATGGAGTC CTCAACATCC ACAGACTCGT CCATCATTGA AGACTCGGTT
    GATTCCCCTT TACAGATTGA GCTGCTGATG AATGCTGTAG ATTTGGAGGC AGAGTCATGG
    AGTTTAGCTG TGGAGGCCCA GTTCTGTAGG ATGCAAGAAA AACGCATCAT CAAGAGACAG
    GATGTGATTT ACGAGTTCAT GCAGACCGAG CTCCATCACG TGCAGACACT CATGGTGATG
    GCAGAGGTCT TCAGAAGGGG AATGCGGGAT GAAGTGGGTC TGGACGCAGA GACCATTGGC
    CGGATTTTTC CTTGCTTAGA TGAGCTACTC CAACTCCATC GTGATTTTCT GACAGCAATG
    AGGGAACGAA GGCAAAGCTG CGTCCAGAAC AACAGCAGCA AAAACTTTCT CATCCACCGT
    GTGGGTGATA TTTTTCTGCA ACAGTTCTCC CAGGAGAACG CAGAGAAGAT GAAGCAGGTG
    TATGGAGAGT TTTGCAGTCA TCACACAGAA GCAGTCAGTT ACTTCAAAGA GCTTCAGCAG
    CAGAACAAGA GATTTCAGCT CTTCATCAAA CAACAGAGTA GTAACTCGCT GGTGAAGAGG
    CGAGAGATCC CAGAATGTAT TTTGTTAGTG ACGCAGCGAA TTACAAAGTA CCCTGTCCTC
    CTAGAGAGAA TACTCCAGTA CACAGACGAG GGCACAGAAG AGCACTCTGA CCTCTCTCGA
    GCTCTAGTCC TGATCCGAGA GCTGATCTCC GCTGTGGACC AATATGTGAG TCAGTATGAG
    CAGAACCAGA AGCTGAGTGA AGTCTTGGGC CGCATGGAGA ACAAGTGCTC TACCAAACTG
    AAGACCGGCT GCACTTTCCG CAAGCAGGAC ATCACCAGCG GCAGGAATCT GCTGCACCAG
    GGCCCGCTGC TCTGGAAGAC AGCCACTGGA AGACTGAAAG ATGTTCTGGC ATTTTTGCTG
    TCTGATTCAC TGGTGTTCCT TCAGGAGAAA GATCAGAAAT ACATTTTTGC TACTGTGGAC
    CAGAAGCCTC CAGTGATCTC TTTGCAGAAA CTAATTGTGC GTGAGGTGGC CAATGAAGAG
    AGAGGCATGT TTCTGATCAG TGCATCTTCA GCCGGGCCTG AGATGTACGA GGTGCATGCA
    GCCTCTAAAG ATGAACGCAA CGCCTGGATG AGACTCATCA GAGAGGCCGT GGAGAATTGC
    CCTGAAGAGG AGGATGAAAC TACAAGTGAG TCAGAAGAGG AAAGGAGAGT CACAGAAGCA
    AAGGTCCAAA AGATCCACCG GCTGCAAGAG ACGCTGTGTG GCCATGATCA CCTCATCTGT
    TCCAATCTGG AGGAGAAGCT GCACATCTAT GCCGAGCTGT CAGTGATGGC AGGCCAGCGA
    GAGGCAGGAC CAGAGCCCAG GCTGCTGGTA CGACCCAGCA CTGAAGACGT TCCCCAGGCT
    GGAGTACTAC TCAGCGCCGC TCTCAGAGAG GCGGAGAGCC TAAGAGCAGC TCTCTCCTCT
    CATTGTGCTT CCACTCCTCC CGTCCCGTCC GGTCCAGAAG ATCTAAACAA ACCTGAACCC
    TGCACTCCTC CATCTCCTCT GCCTCCTGAA AGTCCACTGA CAGACCCCAT CCAGAGTGAG
    GAGGAGGAGT GGAGAACTGA GGAAAGCTCA GAAGCACAGA CCTTTAATCA CAACTCGGAC
    TCTGACCACA ATCTACAGCT GAAGGTGAGT CAGAGTGTGC AGAGCTTGAC TCAGCTGCTG
    TACAGTCTAC AGGCTGCAGT TACCATCCAA GACAGTTGCT ATGAAGTGCA GAAGCTTCTT
    CTCCTGCTGA TGTCAAGCCC CGCCCCTAAC CAACAGTCCC GCCCACTATC CATCACCCGC
    AGCGGGCAGG CTCTGCATGA GCAGGAGCGC CAGCGGGAGG CCGAGCGCCG CCGGGAAGAG
    CTGGCGGATG TGGCTCGTCT GCGGGGAGCC CTGGTACGAG AGCGCCAGCA GTGGGAGAGG
    GAGTGCCAGG CGCGGCAGCT TCAGCACTCA GAACTGGAGA CCACGCTTGA GCAGAGGGAG
    CTCTTGTGCC ACCTAGAGGA GCGGAGGCTG CAGAGCGAGC GGCAGGAGCT CCAGGAGCAG
    CTGCAGGAAT ACCAACAGAG TCTGGAGAGG CTCAGAGAAG GCCAGAGGAA TGTGGAGAAG
    GAGAAGGAGA AACTGGAGAC TCAGAAGATG CTGCTGGAGG GCTTGAAGCA CGGGTGCCAG
    CAGAACCTTC CAGCTATGGT CATACCACTG GACGGACAGC AGACACAGAA TCACCTGGGC
    ATTCAGGACG AGCATGGCTC TATTTTTGTC AATGAGGCAG CGTTCATATC TTCTTCTGTA
    AATAACAGGC ACCTCCATCT TCAGCATCAT CCACAGCATC ATCACCAGAC TGTCCATGTG
    CACTCAGGAT CTATAGGATA CTCAGATTCT CCCAGCGCTC AGAACAGTCT TAATTCACTG
    CTAGCCCGAT CCAACCACAG ACCCAGCTCT GCTGGAGCAT CCGACCACAG CTGGACGTCT
    CTGAGGAGTC TGACAGAGAG TGCAAACAAC AATGTGGGTC AGATCAGCCA TGTTGAAGAG
    TCTTGGTCAG CAGGGGTAAC GGGAGCGGGA CTCCATCAAA CTGATGTCCT GCTCGTCAGC
    CAGCCCTTCC TAAGCATGGA GACAGAGAAC AGAGATACTG ATGGAGAGGA GACCATCGTG
    TATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332208.1
    CDS206..5554
    Translation

    Target ORF information:

    RefSeq Version XM_021476533.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476533.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGATGGCGT TCTCTCCAGC AGCAGTGGCA GCAAGTCTCT TCTACACGCC
    CTGTCTCTGT CTAAATCTCT GTCTCTGTTC AACCCTGTTA AGCACCGCTC TTCCAGCACA
    TCAGAACAGC CTCAAGAGAA AAGGATTGAG GAAGAAGAAT GGGATAAATA TATCATCCCT
    TCCAAAACAG AGTCAGAAAA GTACAAAGTC AGCCGCACAT TCAGCTTCAT AAAGAGCCGA
    ATGTATAGCA CTCGCAACAA GAACAAGGGG AAAGGAAAAG AGAAAGAGAC CAAAGACAAG
    CAGGTCAACG GACACCAGTT CACAGCGGGA TCTGTCCTGG GGTCGACACT CTGTGAGATC
    TGCAGCAAGC CAGCTTCAGG GAAAGAGGTT GTCCATTGCA CACACTGTGC AGTGAGTGTC
    CATAAGGGCT GTAAGGACTC ATCTACGCCA TGCCTGAAGA AACCTCAAGA CAAATATTCA
    GTGATGACAG TAAAAAGCAA GACAGCCTCT CTTCCTCAAA ATTTTATTGT GCGTGAGAGT
    TCATCGTCGT CCCTTCAGAT CTCTACCTCT GCTTCTCTCC CTGTGATGAC ATCACGAGAC
    TCGGCTCCTC TTCCTTGTCC TCTGTCCAGA AGTGTGCCCT TAACCACTGA AAGGCTTAGC
    GAGAGCCCAG AAGCTGATAG TGATTCTAAT CTTTGGAGGA ACCACATCCA ATCAGAGGAG
    CTTCTCCAAA CCATGGAGTC CTCAACATCC ACAGACTCGT CCATCATTGA AGACTCGGTT
    GATTCCCCTT TACAGATTGA GCTGCTGATG AATGCTGTAG ATTTGGAGGC AGAGTCATGG
    AGTTTAGCTG TGGAGGCCCA GTTCTGTAGG ATGCAAGAAA AACGCATCAT CAAGAGACAG
    GATGTGATTT ACGAGTTCAT GCAGACCGAG CTCCATCACG TGCAGACACT CATGGTGATG
    GCAGAGGTCT TCAGAAGGGG AATGCGGGAT GAAGTGGGTC TGGACGCAGA GACCATTGGC
    CGGATTTTTC CTTGCTTAGA TGAGCTACTC CAACTCCATC GTGATTTTCT GACAGCAATG
    AGGGAACGAA GGCAAAGCTG CGTCCAGAAC AACAGCAGCA AAAACTTTCT CATCCACCGT
    GTGGGTGATA TTTTTCTGCA ACAGTTCTCC CAGGAGAACG CAGAGAAGAT GAAGCAGGTG
    TATGGAGAGT TTTGCAGTCA TCACACAGAA GCAGTCAGTT ACTTCAAAGA GCTTCAGCAG
    CAGAACAAGA GATTTCAGCT CTTCATCAAA CAACAGAGTA GTAACTCGCT GGTGAAGAGG
    CGAGAGATCC CAGAATGTAT TTTGTTAGTG ACGCAGCGAA TTACAAAGTA CCCTGTCCTC
    CTAGAGAGAA TACTCCAGTA CACAGACGAG GGCACAGAAG AGCACTCTGA CCTCTCTCGA
    GCTCTAGTCC TGATCCGAGA GCTGATCTCC GCTGTGGACC AATATGTGAG TCAGTATGAG
    CAGAACCAGA AGCTGAGTGA AGTCTTGGGC CGCATGGAGA ACAAGTGCTC TACCAAACTG
    AAGACCGGCT GCACTTTCCG CAAGCAGGAC ATCACCAGCG GCAGGAATCT GCTGCACCAG
    GGCCCGCTGC TCTGGAAGAC AGCCACTGGA AGACTGAAAG ATGTTCTGGC ATTTTTGCTG
    TCTGATTCAC TGGTGTTCCT TCAGGAGAAA GATCAGAAAT ACATTTTTGC TACTGTGGAC
    CAGAAGCCTC CAGTGATCTC TTTGCAGAAA CTAATTGTGC GTGAGGTGGC CAATGAAGAG
    AGAGGCATGT TTCTGATCAG TGCATCTTCA GCCGGGCCTG AGATGTACGA GGTGCATGCA
    GCCTCTAAAG ATGAACGCAA CGCCTGGATG AGACTCATCA GAGAGGCCGT GGAGAATTGC
    CCTGAAGAGG AGGATGAAAC TACAAGTGAG TCAGAAGAGG AAAGGAGAGT CACAGAAGCA
    AAGGTCCAAA AGATCCACCG GCTGCAAGAG ACGCTGTGTG GCCATGATCA CCTCATCTGT
    TCCAATCTGG AGGAGAAGCT GCACATCTAT GCCGAGCTGT CAGTGATGGC AGGCCAGCGA
    GAGGCAGGAC CAGAGCCCAG GCTGCTGGTA CGACCCAGCA CTGAAGACGT TCCCCAGGCT
    GGAGTACTAC TCAGCGCCGC TCTCAGAGAG GCGGAGAGCC TAAGAGCAGC TCTCTCCTCT
    CATTGTGCTT CCACTCCTCC CGTCCCGTCC GGTCCAGAAG ATCTAAACAA ACCTGAACCC
    TGCACTCCTC CATCTCCTCT GCCTCCTGAA AGTCCACTGA CAGACCCCAT CCAGAGTGAG
    GAGGAGGAGT GGAGAACTGA GGAAAGCTCA GAAGCACAGA CCTTTAATCA CAACTCGGAC
    TCTGACCACA ATCTACAGCT GAAGGTGAGT CAGAGTGTGC AGAGCTTGAC TCAGCTGCTG
    TACAGTCTAC AGGCTGCAGT TACCATCCAA GACAGTTGCT ATGAAGTGCA GAAGCTTCTT
    CTCCTGCTGA TGTCAAGCCC CGCCCCTAAC CAACAGTCCC GCCCACTATC CATCACCCGC
    AGCGGGCAGG CTCTGCATGA GCAGGAGCGC CAGCGGGAGG CCGAGCGCCG CCGGGAAGAG
    CTGGCGGATG TGGCTCGTCT GCGGGGAGCC CTGGTACGAG AGCGCCAGCA GTGGGAGAGG
    GAGTGCCAGG CGCGGCAGCT TCAGCACTCA GAACTGGAGA CCACGCTTGA GCAGAGGGAG
    CTCTTGTGCC ACCTAGAGGA GCGGAGGCTG CAGAGCGAGC GGCAGGAGCT CCAGGAGCAG
    CTGCAGGAAT ACCAACAGAG TCTGGAGAGG CTCAGAGAAG GCCAGAGGAA TGTGGAGAAG
    GAGAAGGAGA AACTGGAGAC TCAGAAGATG CTGCTGGAGG GCTTGAAGCA CGGGTGCCAG
    CAGAACCTTC CAGCTATGGT CATACCACTG GACGGACAGC AGACACAGAA TCACCTGGGC
    ATTCAGGACG AGCATGGCTC TATTTTTGTC AATGAGGCAG CGTTCATATC TTCTTCTGTA
    AATAACAGGC ACCTCCATCT TCAGCATCAT CCACAGCATC ATCACCAGAC TGTCCATGTG
    CACTCAGGAT CTATAGGATA CTCAGATTCT CCCAGCGCTC AGAACAGTCT TAATTCACTG
    CTAGCCCGAT CCAACCACAG ACCCAGCTCT GCTGGAGCAT CCGACCACAG CTGGACGTCT
    CTGAGGAGTC TGACAGAGAG TGCAAACAAC AATGTGGGTC AGATCAGCCA TGTTGAAGAG
    TCTTGGTCAG CAGGGGTAAC GGGAGCGGGA CTCCATCAAA CTGATGTCCT GCTCGTCAGC
    CAGCCCTTCC TAAGCATGGA GACAGAGAAC AGAGATACTG ATGGAGAGGA GACCATCGTG
    TATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59222
    Clone ID Related Accession (Same CDS sequence) XM_017356272.1 , XM_021476534.1
    Accession Version XM_017356272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAAC AGCCTCAAGA GAAAAGGATT
    GAGGAAGAAG AATGGGATAA ATATATCATC CCTTCCAAAA CAGAGTCAGA AAAGTACAAA
    GTCAGCCGCA CATTCAGCTT CATAAAGAGC CGAATGTATA GCACTCGCAA CAAGAACAAG
    GGGAAAGGAA AAGAGAAAGA GACCAAAGAC AAGCAGGTCA ACGGACACCA GTTCACAGCG
    GGATCTGTCC TGGGGTCGAC ACTCTGTGAG ATCTGCAGCA AGCCAGCTTC AGGGAAAGAG
    GTTGTCCATT GCACACACTG TGCAGTGAGT GTCCATAAGG GCTGTAAGGA CTCATCTACG
    CCATGCCTGA AGAAACCTCA AGACAAATAT TCAGTGATGA CAGTAAAAAG CAAGACAGCC
    TCTCTTCCTC AAAATTTTAT TGTGCGTGAG AGTTCATCGT CGTCCCTTCA GATCTCTACC
    TCTGCTTCTC TCCCTGTGAT GACATCACGA GACTCGGCTC CTCTTCCTTG TCCTCTGTCC
    AGAAGTGTGC CCTTAACCAC TGAAAGGCTT AGCGAGAGCC CAGAAGCTGA TAGTGATTCT
    AATCTTTGGA GGAACCACAT CCAATCAGAG GAGCTTCTCC AAACCATGGA GTCCTCAACA
    TCCACAGACT CGTCCATCAT TGAAGACTCG GTTGATTCCC CTTTACAGAT TGAGCTGCTG
    ATGAATGCTG TAGATTTGGA GGCAGAGTCA TGGAGTTTAG CTGTGGAGGC CCAGTTCTGT
    AGGATGCAAG AAAAACGCAT CATCAAGAGA CAGGATGTGA TTTACGAGTT CATGCAGACC
    GAGCTCCATC ACGTGCAGAC ACTCATGGTG ATGGCAGAGG TCTTCAGAAG GGGAATGCGG
    GATGAAGTGG GTCTGGACGC AGAGACCATT GGCCGGATTT TTCCTTGCTT AGATGAGCTA
    CTCCAACTCC ATCGTGATTT TCTGACAGCA ATGAGGGAAC GAAGGCAAAG CTGCGTCCAG
    AACAACAGCA GCAAAAACTT TCTCATCCAC CGTGTGGGTG ATATTTTTCT GCAACAGTTC
    TCCCAGGAGA ACGCAGAGAA GATGAAGCAG GTGTATGGAG AGTTTTGCAG TCATCACACA
    GAAGCAGTCA GTTACTTCAA AGAGCTTCAG CAGCAGAACA AGAGATTTCA GCTCTTCATC
    AAACAACAGA GTAGTAACTC GCTGGTGAAG AGGCGAGAGA TCCCAGAATG TATTTTGTTA
    GTGACGCAGC GAATTACAAA GTACCCTGTC CTCCTAGAGA GAATACTCCA GTACACAGAC
    GAGGGCACAG AAGAGCACTC TGACCTCTCT CGAGCTCTAG TCCTGATCCG AGAGCTGATC
    TCCGCTGTGG ACCAATATGT GAGTCAGTAT GAGCAGAACC AGAAGCTGAG TGAAGTCTTG
    GGCCGCATGG AGAACAAGTG CTCTACCAAA CTGAAGACCG GCTGCACTTT CCGCAAGCAG
    GACATCACCA GCGGCAGGAA TCTGCTGCAC CAGGGCCCGC TGCTCTGGAA GACAGCCACT
    GGAAGACTGA AAGATGTTCT GGCATTTTTG CTGTCTGATT CACTGGTGTT CCTTCAGGAG
    AAAGATCAGA AATACATTTT TGCTACTGTG GACCAGAAGC CTCCAGTGAT CTCTTTGCAG
    AAACTAATTG TGCGTGAGGT GGCCAATGAA GAGAGAGGCA TGTTTCTGAT CAGTGCATCT
    TCAGCCGGGC CTGAGATGTA CGAGGTGCAT GCAGCCTCTA AAGATGAACG CAACGCCTGG
    ATGAGACTCA TCAGAGAGGC CGTGGAGAAT TGCCCTGAAG AGGAGGATGA AACTACAAGT
    GAGTCAGAAG AGGAAAGGAG AGTCACAGAA GCAAAGGTCC AAAAGATCCA CCGGCTGCAA
    GAGACGCTGT GTGGCCATGA TCACCTCATC TGTTCCAATC TGGAGGAGAA GCTGCACATC
    TATGCCGAGC TGTCAGTGAT GGCAGGCCAG CGAGAGGCAG GACCAGAGCC CAGGCTGCTG
    GTACGACCCA GCACTGAAGA CGTTCCCCAG GCTGGAGTAC TACTCAGCGC CGCTCTCAGA
    GAGGCGGAGA GCCTAAGAGC AGCTCTCTCC TCTCATTGTG CTTCCACTCC TCCCGTCCCG
    TCCGGTCCAG AAGATCTAAA CAAACCTGAA CCCTGCACTC CTCCATCTCC TCTGCCTCCT
    GAAAGTCCAC TGACAGACCC CATCCAGAGT GAGGAGGAGG AGTGGAGAAC TGAGGAAAGC
    TCAGAAGCAC AGACCTTTAA TCACAACTCG GACTCTGACC ACAATCTACA GCTGAAGGTG
    AGTCAGAGTG TGCAGAGCTT GACTCAGCTG CTGTACAGTC TACAGGCTGC AGTTACCATC
    CAAGACAGTT GCTATGAAGT GCAGAAGCTT CTTCTCCTGC TGATGTCAAG CCCCGCCCCT
    AACCAACAGT CCCGCCCACT ATCCATCACC CGCAGCGGGC AGGCTCTGCA TGAGCAGGAG
    CGCCAGCGGG AGGCCGAGCG CCGCCGGGAA GAGCTGGCGG ATGTGGCTCG TCTGCGGGGA
    GCCCTGGTAC GAGAGCGCCA GCAGTGGGAG AGGGAGTGCC AGGCGCGGCA GCTTCAGCAC
    TCAGAACTGG AGACCACGCT TGAGCAGAGG GAGCTCTTGT GCCACCTAGA GGAGCGGAGG
    CTGCAGAGCG AGCGGCAGGA GCTCCAGGAG CAGCTGCAGG AATACCAACA GAGTCTGGAG
    AGGCTCAGAG AAGGCCAGAG GAATGTGGAG AAGGAGAAGG AGAAACTGGA GACTCAGAAG
    ATGCTGCTGG AGGGCTTGAA GCACGGGTGC CAGCAGAACC TTCCAGCTAT GGTCATACCA
    CTGGACGGAC AGCAGACACA GAATCACCTG GGCATTCAGG ACGAGCATGG CTCTATTTTT
    GTCAATGAGG CAGCGTTCAT ATCTTCTTCT GTAAATAACA GGCACCTCCA TCTTCAGCAT
    CATCCACAGC ATCATCACCA GACTGTCCAT GTGCACTCAG GATCTATAGG ATACTCAGAT
    TCTCCCAGCG CTCAGAACAG TCTTAATTCA CTGCTAGCCC GATCCAACCA CAGACCCAGC
    TCTGCTGGAG CATCCGACCA CAGCTGGACG TCTCTGAGGA GTCTGACAGA GAGTGCAAAC
    AACAATGTGG GTCAGATCAG CCATGTTGAA GAGTCTTGGT CAGCAGGGGT AACGGGAGCG
    GGACTCCATC AAACTGATGT CCTGCTCGTC AGCCAGCCCT TCCTAAGCAT GGAGACAGAG
    AACAGAGATA CTGATGGAGA GGAGACCATC GTGTATCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211761.1
    CDS197..5458
    Translation

    Target ORF information:

    RefSeq Version XM_017356272.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAAC AGCCTCAAGA GAAAAGGATT
    GAGGAAGAAG AATGGGATAA ATATATCATC CCTTCCAAAA CAGAGTCAGA AAAGTACAAA
    GTCAGCCGCA CATTCAGCTT CATAAAGAGC CGAATGTATA GCACTCGCAA CAAGAACAAG
    GGGAAAGGAA AAGAGAAAGA GACCAAAGAC AAGCAGGTCA ACGGACACCA GTTCACAGCG
    GGATCTGTCC TGGGGTCGAC ACTCTGTGAG ATCTGCAGCA AGCCAGCTTC AGGGAAAGAG
    GTTGTCCATT GCACACACTG TGCAGTGAGT GTCCATAAGG GCTGTAAGGA CTCATCTACG
    CCATGCCTGA AGAAACCTCA AGACAAATAT TCAGTGATGA CAGTAAAAAG CAAGACAGCC
    TCTCTTCCTC AAAATTTTAT TGTGCGTGAG AGTTCATCGT CGTCCCTTCA GATCTCTACC
    TCTGCTTCTC TCCCTGTGAT GACATCACGA GACTCGGCTC CTCTTCCTTG TCCTCTGTCC
    AGAAGTGTGC CCTTAACCAC TGAAAGGCTT AGCGAGAGCC CAGAAGCTGA TAGTGATTCT
    AATCTTTGGA GGAACCACAT CCAATCAGAG GAGCTTCTCC AAACCATGGA GTCCTCAACA
    TCCACAGACT CGTCCATCAT TGAAGACTCG GTTGATTCCC CTTTACAGAT TGAGCTGCTG
    ATGAATGCTG TAGATTTGGA GGCAGAGTCA TGGAGTTTAG CTGTGGAGGC CCAGTTCTGT
    AGGATGCAAG AAAAACGCAT CATCAAGAGA CAGGATGTGA TTTACGAGTT CATGCAGACC
    GAGCTCCATC ACGTGCAGAC ACTCATGGTG ATGGCAGAGG TCTTCAGAAG GGGAATGCGG
    GATGAAGTGG GTCTGGACGC AGAGACCATT GGCCGGATTT TTCCTTGCTT AGATGAGCTA
    CTCCAACTCC ATCGTGATTT TCTGACAGCA ATGAGGGAAC GAAGGCAAAG CTGCGTCCAG
    AACAACAGCA GCAAAAACTT TCTCATCCAC CGTGTGGGTG ATATTTTTCT GCAACAGTTC
    TCCCAGGAGA ACGCAGAGAA GATGAAGCAG GTGTATGGAG AGTTTTGCAG TCATCACACA
    GAAGCAGTCA GTTACTTCAA AGAGCTTCAG CAGCAGAACA AGAGATTTCA GCTCTTCATC
    AAACAACAGA GTAGTAACTC GCTGGTGAAG AGGCGAGAGA TCCCAGAATG TATTTTGTTA
    GTGACGCAGC GAATTACAAA GTACCCTGTC CTCCTAGAGA GAATACTCCA GTACACAGAC
    GAGGGCACAG AAGAGCACTC TGACCTCTCT CGAGCTCTAG TCCTGATCCG AGAGCTGATC
    TCCGCTGTGG ACCAATATGT GAGTCAGTAT GAGCAGAACC AGAAGCTGAG TGAAGTCTTG
    GGCCGCATGG AGAACAAGTG CTCTACCAAA CTGAAGACCG GCTGCACTTT CCGCAAGCAG
    GACATCACCA GCGGCAGGAA TCTGCTGCAC CAGGGCCCGC TGCTCTGGAA GACAGCCACT
    GGAAGACTGA AAGATGTTCT GGCATTTTTG CTGTCTGATT CACTGGTGTT CCTTCAGGAG
    AAAGATCAGA AATACATTTT TGCTACTGTG GACCAGAAGC CTCCAGTGAT CTCTTTGCAG
    AAACTAATTG TGCGTGAGGT GGCCAATGAA GAGAGAGGCA TGTTTCTGAT CAGTGCATCT
    TCAGCCGGGC CTGAGATGTA CGAGGTGCAT GCAGCCTCTA AAGATGAACG CAACGCCTGG
    ATGAGACTCA TCAGAGAGGC CGTGGAGAAT TGCCCTGAAG AGGAGGATGA AACTACAAGT
    GAGTCAGAAG AGGAAAGGAG AGTCACAGAA GCAAAGGTCC AAAAGATCCA CCGGCTGCAA
    GAGACGCTGT GTGGCCATGA TCACCTCATC TGTTCCAATC TGGAGGAGAA GCTGCACATC
    TATGCCGAGC TGTCAGTGAT GGCAGGCCAG CGAGAGGCAG GACCAGAGCC CAGGCTGCTG
    GTACGACCCA GCACTGAAGA CGTTCCCCAG GCTGGAGTAC TACTCAGCGC CGCTCTCAGA
    GAGGCGGAGA GCCTAAGAGC AGCTCTCTCC TCTCATTGTG CTTCCACTCC TCCCGTCCCG
    TCCGGTCCAG AAGATCTAAA CAAACCTGAA CCCTGCACTC CTCCATCTCC TCTGCCTCCT
    GAAAGTCCAC TGACAGACCC CATCCAGAGT GAGGAGGAGG AGTGGAGAAC TGAGGAAAGC
    TCAGAAGCAC AGACCTTTAA TCACAACTCG GACTCTGACC ACAATCTACA GCTGAAGGTG
    AGTCAGAGTG TGCAGAGCTT GACTCAGCTG CTGTACAGTC TACAGGCTGC AGTTACCATC
    CAAGACAGTT GCTATGAAGT GCAGAAGCTT CTTCTCCTGC TGATGTCAAG CCCCGCCCCT
    AACCAACAGT CCCGCCCACT ATCCATCACC CGCAGCGGGC AGGCTCTGCA TGAGCAGGAG
    CGCCAGCGGG AGGCCGAGCG CCGCCGGGAA GAGCTGGCGG ATGTGGCTCG TCTGCGGGGA
    GCCCTGGTAC GAGAGCGCCA GCAGTGGGAG AGGGAGTGCC AGGCGCGGCA GCTTCAGCAC
    TCAGAACTGG AGACCACGCT TGAGCAGAGG GAGCTCTTGT GCCACCTAGA GGAGCGGAGG
    CTGCAGAGCG AGCGGCAGGA GCTCCAGGAG CAGCTGCAGG AATACCAACA GAGTCTGGAG
    AGGCTCAGAG AAGGCCAGAG GAATGTGGAG AAGGAGAAGG AGAAACTGGA GACTCAGAAG
    ATGCTGCTGG AGGGCTTGAA GCACGGGTGC CAGCAGAACC TTCCAGCTAT GGTCATACCA
    CTGGACGGAC AGCAGACACA GAATCACCTG GGCATTCAGG ACGAGCATGG CTCTATTTTT
    GTCAATGAGG CAGCGTTCAT ATCTTCTTCT GTAAATAACA GGCACCTCCA TCTTCAGCAT
    CATCCACAGC ATCATCACCA GACTGTCCAT GTGCACTCAG GATCTATAGG ATACTCAGAT
    TCTCCCAGCG CTCAGAACAG TCTTAATTCA CTGCTAGCCC GATCCAACCA CAGACCCAGC
    TCTGCTGGAG CATCCGACCA CAGCTGGACG TCTCTGAGGA GTCTGACAGA GAGTGCAAAC
    AACAATGTGG GTCAGATCAG CCATGTTGAA GAGTCTTGGT CAGCAGGGGT AACGGGAGCG
    GGACTCCATC AAACTGATGT CCTGCTCGTC AGCCAGCCCT TCCTAAGCAT GGAGACAGAG
    AACAGAGATA CTGATGGAGA GGAGACCATC GTGTATCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59222
    Clone ID Related Accession (Same CDS sequence) XM_017356272.1 , XM_021476534.1
    Accession Version XM_021476534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAAC AGCCTCAAGA GAAAAGGATT
    GAGGAAGAAG AATGGGATAA ATATATCATC CCTTCCAAAA CAGAGTCAGA AAAGTACAAA
    GTCAGCCGCA CATTCAGCTT CATAAAGAGC CGAATGTATA GCACTCGCAA CAAGAACAAG
    GGGAAAGGAA AAGAGAAAGA GACCAAAGAC AAGCAGGTCA ACGGACACCA GTTCACAGCG
    GGATCTGTCC TGGGGTCGAC ACTCTGTGAG ATCTGCAGCA AGCCAGCTTC AGGGAAAGAG
    GTTGTCCATT GCACACACTG TGCAGTGAGT GTCCATAAGG GCTGTAAGGA CTCATCTACG
    CCATGCCTGA AGAAACCTCA AGACAAATAT TCAGTGATGA CAGTAAAAAG CAAGACAGCC
    TCTCTTCCTC AAAATTTTAT TGTGCGTGAG AGTTCATCGT CGTCCCTTCA GATCTCTACC
    TCTGCTTCTC TCCCTGTGAT GACATCACGA GACTCGGCTC CTCTTCCTTG TCCTCTGTCC
    AGAAGTGTGC CCTTAACCAC TGAAAGGCTT AGCGAGAGCC CAGAAGCTGA TAGTGATTCT
    AATCTTTGGA GGAACCACAT CCAATCAGAG GAGCTTCTCC AAACCATGGA GTCCTCAACA
    TCCACAGACT CGTCCATCAT TGAAGACTCG GTTGATTCCC CTTTACAGAT TGAGCTGCTG
    ATGAATGCTG TAGATTTGGA GGCAGAGTCA TGGAGTTTAG CTGTGGAGGC CCAGTTCTGT
    AGGATGCAAG AAAAACGCAT CATCAAGAGA CAGGATGTGA TTTACGAGTT CATGCAGACC
    GAGCTCCATC ACGTGCAGAC ACTCATGGTG ATGGCAGAGG TCTTCAGAAG GGGAATGCGG
    GATGAAGTGG GTCTGGACGC AGAGACCATT GGCCGGATTT TTCCTTGCTT AGATGAGCTA
    CTCCAACTCC ATCGTGATTT TCTGACAGCA ATGAGGGAAC GAAGGCAAAG CTGCGTCCAG
    AACAACAGCA GCAAAAACTT TCTCATCCAC CGTGTGGGTG ATATTTTTCT GCAACAGTTC
    TCCCAGGAGA ACGCAGAGAA GATGAAGCAG GTGTATGGAG AGTTTTGCAG TCATCACACA
    GAAGCAGTCA GTTACTTCAA AGAGCTTCAG CAGCAGAACA AGAGATTTCA GCTCTTCATC
    AAACAACAGA GTAGTAACTC GCTGGTGAAG AGGCGAGAGA TCCCAGAATG TATTTTGTTA
    GTGACGCAGC GAATTACAAA GTACCCTGTC CTCCTAGAGA GAATACTCCA GTACACAGAC
    GAGGGCACAG AAGAGCACTC TGACCTCTCT CGAGCTCTAG TCCTGATCCG AGAGCTGATC
    TCCGCTGTGG ACCAATATGT GAGTCAGTAT GAGCAGAACC AGAAGCTGAG TGAAGTCTTG
    GGCCGCATGG AGAACAAGTG CTCTACCAAA CTGAAGACCG GCTGCACTTT CCGCAAGCAG
    GACATCACCA GCGGCAGGAA TCTGCTGCAC CAGGGCCCGC TGCTCTGGAA GACAGCCACT
    GGAAGACTGA AAGATGTTCT GGCATTTTTG CTGTCTGATT CACTGGTGTT CCTTCAGGAG
    AAAGATCAGA AATACATTTT TGCTACTGTG GACCAGAAGC CTCCAGTGAT CTCTTTGCAG
    AAACTAATTG TGCGTGAGGT GGCCAATGAA GAGAGAGGCA TGTTTCTGAT CAGTGCATCT
    TCAGCCGGGC CTGAGATGTA CGAGGTGCAT GCAGCCTCTA AAGATGAACG CAACGCCTGG
    ATGAGACTCA TCAGAGAGGC CGTGGAGAAT TGCCCTGAAG AGGAGGATGA AACTACAAGT
    GAGTCAGAAG AGGAAAGGAG AGTCACAGAA GCAAAGGTCC AAAAGATCCA CCGGCTGCAA
    GAGACGCTGT GTGGCCATGA TCACCTCATC TGTTCCAATC TGGAGGAGAA GCTGCACATC
    TATGCCGAGC TGTCAGTGAT GGCAGGCCAG CGAGAGGCAG GACCAGAGCC CAGGCTGCTG
    GTACGACCCA GCACTGAAGA CGTTCCCCAG GCTGGAGTAC TACTCAGCGC CGCTCTCAGA
    GAGGCGGAGA GCCTAAGAGC AGCTCTCTCC TCTCATTGTG CTTCCACTCC TCCCGTCCCG
    TCCGGTCCAG AAGATCTAAA CAAACCTGAA CCCTGCACTC CTCCATCTCC TCTGCCTCCT
    GAAAGTCCAC TGACAGACCC CATCCAGAGT GAGGAGGAGG AGTGGAGAAC TGAGGAAAGC
    TCAGAAGCAC AGACCTTTAA TCACAACTCG GACTCTGACC ACAATCTACA GCTGAAGGTG
    AGTCAGAGTG TGCAGAGCTT GACTCAGCTG CTGTACAGTC TACAGGCTGC AGTTACCATC
    CAAGACAGTT GCTATGAAGT GCAGAAGCTT CTTCTCCTGC TGATGTCAAG CCCCGCCCCT
    AACCAACAGT CCCGCCCACT ATCCATCACC CGCAGCGGGC AGGCTCTGCA TGAGCAGGAG
    CGCCAGCGGG AGGCCGAGCG CCGCCGGGAA GAGCTGGCGG ATGTGGCTCG TCTGCGGGGA
    GCCCTGGTAC GAGAGCGCCA GCAGTGGGAG AGGGAGTGCC AGGCGCGGCA GCTTCAGCAC
    TCAGAACTGG AGACCACGCT TGAGCAGAGG GAGCTCTTGT GCCACCTAGA GGAGCGGAGG
    CTGCAGAGCG AGCGGCAGGA GCTCCAGGAG CAGCTGCAGG AATACCAACA GAGTCTGGAG
    AGGCTCAGAG AAGGCCAGAG GAATGTGGAG AAGGAGAAGG AGAAACTGGA GACTCAGAAG
    ATGCTGCTGG AGGGCTTGAA GCACGGGTGC CAGCAGAACC TTCCAGCTAT GGTCATACCA
    CTGGACGGAC AGCAGACACA GAATCACCTG GGCATTCAGG ACGAGCATGG CTCTATTTTT
    GTCAATGAGG CAGCGTTCAT ATCTTCTTCT GTAAATAACA GGCACCTCCA TCTTCAGCAT
    CATCCACAGC ATCATCACCA GACTGTCCAT GTGCACTCAG GATCTATAGG ATACTCAGAT
    TCTCCCAGCG CTCAGAACAG TCTTAATTCA CTGCTAGCCC GATCCAACCA CAGACCCAGC
    TCTGCTGGAG CATCCGACCA CAGCTGGACG TCTCTGAGGA GTCTGACAGA GAGTGCAAAC
    AACAATGTGG GTCAGATCAG CCATGTTGAA GAGTCTTGGT CAGCAGGGGT AACGGGAGCG
    GGACTCCATC AAACTGATGT CCTGCTCGTC AGCCAGCCCT TCCTAAGCAT GGAGACAGAG
    AACAGAGATA CTGATGGAGA GGAGACCATC GTGTATCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332209.1
    CDS206..5467
    Translation

    Target ORF information:

    RefSeq Version XM_021476534.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GAAGTCCAAT CCTCTCTCTA
    AGCCCGAGTC TGGTTGCCTT GGAAATGGAC AGCGAGGAGG ATGACGATGA TGACATTTTA
    ATGAAGAAGC CCCTCCCACA ACTGCAACCG GATTCCAAAG ACAGCACAGA TGAGCAGGTT
    ATGTCCAGCC CCGCACCTGT ACTCATCTGC ACGCGCTCTC ACTCTGCCTC CTCTGCCTGT
    AACCCCGCCC CTGCAAAGGA TGTTGAGCAG GGTGTCCGAC TCCGATCCTA TTCCTACACC
    TCCCCCAAAA TCAGCCTTCT GCCTCCACGT TTCAGCCGAG ACGCCCATCC TCCACCCGCC
    GACCTCAGCC AGGAGCACCG CTCTTCCAGC ACATCAGAAC AGCCTCAAGA GAAAAGGATT
    GAGGAAGAAG AATGGGATAA ATATATCATC CCTTCCAAAA CAGAGTCAGA AAAGTACAAA
    GTCAGCCGCA CATTCAGCTT CATAAAGAGC CGAATGTATA GCACTCGCAA CAAGAACAAG
    GGGAAAGGAA AAGAGAAAGA GACCAAAGAC AAGCAGGTCA ACGGACACCA GTTCACAGCG
    GGATCTGTCC TGGGGTCGAC ACTCTGTGAG ATCTGCAGCA AGCCAGCTTC AGGGAAAGAG
    GTTGTCCATT GCACACACTG TGCAGTGAGT GTCCATAAGG GCTGTAAGGA CTCATCTACG
    CCATGCCTGA AGAAACCTCA AGACAAATAT TCAGTGATGA CAGTAAAAAG CAAGACAGCC
    TCTCTTCCTC AAAATTTTAT TGTGCGTGAG AGTTCATCGT CGTCCCTTCA GATCTCTACC
    TCTGCTTCTC TCCCTGTGAT GACATCACGA GACTCGGCTC CTCTTCCTTG TCCTCTGTCC
    AGAAGTGTGC CCTTAACCAC TGAAAGGCTT AGCGAGAGCC CAGAAGCTGA TAGTGATTCT
    AATCTTTGGA GGAACCACAT CCAATCAGAG GAGCTTCTCC AAACCATGGA GTCCTCAACA
    TCCACAGACT CGTCCATCAT TGAAGACTCG GTTGATTCCC CTTTACAGAT TGAGCTGCTG
    ATGAATGCTG TAGATTTGGA GGCAGAGTCA TGGAGTTTAG CTGTGGAGGC CCAGTTCTGT
    AGGATGCAAG AAAAACGCAT CATCAAGAGA CAGGATGTGA TTTACGAGTT CATGCAGACC
    GAGCTCCATC ACGTGCAGAC ACTCATGGTG ATGGCAGAGG TCTTCAGAAG GGGAATGCGG
    GATGAAGTGG GTCTGGACGC AGAGACCATT GGCCGGATTT TTCCTTGCTT AGATGAGCTA
    CTCCAACTCC ATCGTGATTT TCTGACAGCA ATGAGGGAAC GAAGGCAAAG CTGCGTCCAG
    AACAACAGCA GCAAAAACTT TCTCATCCAC CGTGTGGGTG ATATTTTTCT GCAACAGTTC
    TCCCAGGAGA ACGCAGAGAA GATGAAGCAG GTGTATGGAG AGTTTTGCAG TCATCACACA
    GAAGCAGTCA GTTACTTCAA AGAGCTTCAG CAGCAGAACA AGAGATTTCA GCTCTTCATC
    AAACAACAGA GTAGTAACTC GCTGGTGAAG AGGCGAGAGA TCCCAGAATG TATTTTGTTA
    GTGACGCAGC GAATTACAAA GTACCCTGTC CTCCTAGAGA GAATACTCCA GTACACAGAC
    GAGGGCACAG AAGAGCACTC TGACCTCTCT CGAGCTCTAG TCCTGATCCG AGAGCTGATC
    TCCGCTGTGG ACCAATATGT GAGTCAGTAT GAGCAGAACC AGAAGCTGAG TGAAGTCTTG
    GGCCGCATGG AGAACAAGTG CTCTACCAAA CTGAAGACCG GCTGCACTTT CCGCAAGCAG
    GACATCACCA GCGGCAGGAA TCTGCTGCAC CAGGGCCCGC TGCTCTGGAA GACAGCCACT
    GGAAGACTGA AAGATGTTCT GGCATTTTTG CTGTCTGATT CACTGGTGTT CCTTCAGGAG
    AAAGATCAGA AATACATTTT TGCTACTGTG GACCAGAAGC CTCCAGTGAT CTCTTTGCAG
    AAACTAATTG TGCGTGAGGT GGCCAATGAA GAGAGAGGCA TGTTTCTGAT CAGTGCATCT
    TCAGCCGGGC CTGAGATGTA CGAGGTGCAT GCAGCCTCTA AAGATGAACG CAACGCCTGG
    ATGAGACTCA TCAGAGAGGC CGTGGAGAAT TGCCCTGAAG AGGAGGATGA AACTACAAGT
    GAGTCAGAAG AGGAAAGGAG AGTCACAGAA GCAAAGGTCC AAAAGATCCA CCGGCTGCAA
    GAGACGCTGT GTGGCCATGA TCACCTCATC TGTTCCAATC TGGAGGAGAA GCTGCACATC
    TATGCCGAGC TGTCAGTGAT GGCAGGCCAG CGAGAGGCAG GACCAGAGCC CAGGCTGCTG
    GTACGACCCA GCACTGAAGA CGTTCCCCAG GCTGGAGTAC TACTCAGCGC CGCTCTCAGA
    GAGGCGGAGA GCCTAAGAGC AGCTCTCTCC TCTCATTGTG CTTCCACTCC TCCCGTCCCG
    TCCGGTCCAG AAGATCTAAA CAAACCTGAA CCCTGCACTC CTCCATCTCC TCTGCCTCCT
    GAAAGTCCAC TGACAGACCC CATCCAGAGT GAGGAGGAGG AGTGGAGAAC TGAGGAAAGC
    TCAGAAGCAC AGACCTTTAA TCACAACTCG GACTCTGACC ACAATCTACA GCTGAAGGTG
    AGTCAGAGTG TGCAGAGCTT GACTCAGCTG CTGTACAGTC TACAGGCTGC AGTTACCATC
    CAAGACAGTT GCTATGAAGT GCAGAAGCTT CTTCTCCTGC TGATGTCAAG CCCCGCCCCT
    AACCAACAGT CCCGCCCACT ATCCATCACC CGCAGCGGGC AGGCTCTGCA TGAGCAGGAG
    CGCCAGCGGG AGGCCGAGCG CCGCCGGGAA GAGCTGGCGG ATGTGGCTCG TCTGCGGGGA
    GCCCTGGTAC GAGAGCGCCA GCAGTGGGAG AGGGAGTGCC AGGCGCGGCA GCTTCAGCAC
    TCAGAACTGG AGACCACGCT TGAGCAGAGG GAGCTCTTGT GCCACCTAGA GGAGCGGAGG
    CTGCAGAGCG AGCGGCAGGA GCTCCAGGAG CAGCTGCAGG AATACCAACA GAGTCTGGAG
    AGGCTCAGAG AAGGCCAGAG GAATGTGGAG AAGGAGAAGG AGAAACTGGA GACTCAGAAG
    ATGCTGCTGG AGGGCTTGAA GCACGGGTGC CAGCAGAACC TTCCAGCTAT GGTCATACCA
    CTGGACGGAC AGCAGACACA GAATCACCTG GGCATTCAGG ACGAGCATGG CTCTATTTTT
    GTCAATGAGG CAGCGTTCAT ATCTTCTTCT GTAAATAACA GGCACCTCCA TCTTCAGCAT
    CATCCACAGC ATCATCACCA GACTGTCCAT GTGCACTCAG GATCTATAGG ATACTCAGAT
    TCTCCCAGCG CTCAGAACAG TCTTAATTCA CTGCTAGCCC GATCCAACCA CAGACCCAGC
    TCTGCTGGAG CATCCGACCA CAGCTGGACG TCTCTGAGGA GTCTGACAGA GAGTGCAAAC
    AACAATGTGG GTCAGATCAG CCATGTTGAA GAGTCTTGGT CAGCAGGGGT AACGGGAGCG
    GGACTCCATC AAACTGATGT CCTGCTCGTC AGCCAGCCCT TCCTAAGCAT GGAGACAGAG
    AACAGAGATA CTGATGGAGA GGAGACCATC GTGTATCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43200
    Clone ID Related Accession (Same CDS sequence) XM_021476530.1 , XM_009301598.2
    Accession Version XM_021476530.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GCCCGAGTCT GGTTGCCTTG
    GAAATGGACA GCGAGGAGGA TGACGATGAT GACATTTTAA TGAAGAAGCC CCTCCCACAA
    CTGCAACCGG ATTCCAAAGA CAGCACAGAT GAGCAGGTTA TGTCCAGCCC CGCACCTGTA
    CTCATCTGCA CGCGCTCTCA CTCTGCCTCC TCTGCCTGTA ACCCCGCCCC TGCAAAGGAT
    GTTGAGCAGG GTGTCCGACT CCGATCCTAT TCCTACACCT CCCCCAAAAT CAGCCTTCTG
    CCTCCACGTT TCAGCCGAGA CGCCCATCCT CCACCCGCCG ACCTCAGCCA GGATGGCGTT
    CTCTCCAGCA GCAGTGGCAG CAAGTCTCTT CTACACGCCC TGTCTCTGTC TAAATCTCTG
    TCTCTGTTCA ACCCTGTTAA GCACCGCTCT TCCAGCACAT CAGAACAGCC TCAAGAGAAA
    AGGGAGTTAA GGTTTCGCAG GCGAGCTCAG TCGGCTGATG ATGAAAGCAG TGCAGAGCTG
    GCCGACTCGC TCCAACATCT CACGCTCTCA GAGTTCCTCA AAGAGATTGA GGAAGAAGAA
    TGGGATAAAT ATATCATCCC TTCCAAAACA GAGTCAGAAA AGTACAAAGT CAGCCGCACA
    TTCAGCTTCA TAAAGAGCCG AATGTATAGC ACTCGCAACA AGAACAAGGG GAAAGGAAAA
    GAGAAAGAGA CCAAAGACAA GCAGGTCAAC GGACACCAGT TCACAGCGGG ATCTGTCCTG
    GGGTCGACAC TCTGTGAGAT CTGCAGCAAG CCAGCTTCAG GGAAAGAGGT TGTCCATTGC
    ACACACTGTG CAGTGAGTGT CCATAAGGGC TGTAAGGACT CATCTACGCC ATGCCTGAAG
    AAACCTCAAG ACAAATATTC AGTGATGACA GTAAAAAGCA AGACAGCCTC TCTTCCTCAA
    AATTTTATTG TGCGTGAGAG TTCATCGTCG TCCCTTCAGA TCTCTACCTC TGCTTCTCTC
    CCTGTGATGA CATCACGAGA CTCGGCTCCT CTTCCTTGTC CTCTGTCCAG AAGTGTGCCC
    TTAACCACTG AAAGGCTTAG CGAGAGCCCA GAAGCTGATA GTGATTCTAA TCTTTGGAGG
    AACCACATCC AATCAGAGGA GCTTCTCCAA ACCATGGAGT CCTCAACATC CACAGACTCG
    TCCATCATTG AAGACTCGGT TGATTCCCCT TTACAGATTG AGCTGCTGAT GAATGCTGTA
    GATTTGGAGG CAGAGTCATG GAGTTTAGCT GTGGAGGCCC AGTTCTGTAG GATGCAAGAA
    AAACGCATCA TCAAGAGACA GGATGTGATT TACGAGTTCA TGCAGACCGA GCTCCATCAC
    GTGCAGACAC TCATGGTGAT GGCAGAGGTC TTCAGAAGGG GAATGCGGGA TGAAGTGGGT
    CTGGACGCAG AGACCATTGG CCGGATTTTT CCTTGCTTAG ATGAGCTACT CCAACTCCAT
    CGTGATTTTC TGACAGCAAT GAGGGAACGA AGGCAAAGCT GCGTCCAGAA CAACAGCAGC
    AAAAACTTTC TCATCCACCG TGTGGGTGAT ATTTTTCTGC AACAGTTCTC CCAGGAGAAC
    GCAGAGAAGA TGAAGCAGGT GTATGGAGAG TTTTGCAGTC ATCACACAGA AGCAGTCAGT
    TACTTCAAAG AGCTTCAGCA GCAGAACAAG AGATTTCAGC TCTTCATCAA ACAACAGAGT
    AGTAACTCGC TGGTGAAGAG GCGAGAGATC CCAGAATGTA TTTTGTTAGT GACGCAGCGA
    ATTACAAAGT ACCCTGTCCT CCTAGAGAGA ATACTCCAGT ACACAGACGA GGGCACAGAA
    GAGCACTCTG ACCTCTCTCG AGCTCTAGTC CTGATCCGAG AGCTGATCTC CGCTGTGGAC
    CAATATGTGA GTCAGTATGA GCAGAACCAG AAGCTGAGTG AAGTCTTGGG CCGCATGGAG
    AACAAGTGCT CTACCAAACT GAAGACCGGC TGCACTTTCC GCAAGCAGGA CATCACCAGC
    GGCAGGAATC TGCTGCACCA GGGCCCGCTG CTCTGGAAGA CAGCCACTGG AAGACTGAAA
    GATGTTCTGG CATTTTTGCT GTCTGATTCA CTGGTGTTCC TTCAGGAGAA AGATCAGAAA
    TACATTTTTG CTACTGTGGA CCAGAAGCCT CCAGTGATCT CTTTGCAGAA ACTAATTGTG
    CGTGAGGTGG CCAATGAAGA GAGAGGCATG TTTCTGATCA GTGCATCTTC AGCCGGGCCT
    GAGATGTACG AGGTGCATGC AGCCTCTAAA GATGAACGCA ACGCCTGGAT GAGACTCATC
    AGAGAGGCCG TGGAGAATTG CCCTGAAGAG GAGGATGAAA CTACAAGTGA GTCAGAAGAG
    GAAAGGAGAG TCACAGAAGC AAAGGTCCAA AAGATCCACC GGCTGCAAGA GACGCTGTGT
    GGCCATGATC ACCTCATCTG TTCCAATCTG GAGGAGAAGC TGCACATCTA TGCCGAGCTG
    TCAGTGATGG CAGGCCAGCG AGAGGCAGGA CCAGAGCCCA GGCTGCTGGT ACGACCCAGC
    ACTGAAGACG TTCCCCAGGC TGGAGTACTA CTCAGCGCCG CTCTCAGAGA GGCGGAGAGC
    CTAAGAGCAG CTCTCTCCTC TCATTGTGCT TCCACTCCTC CCGTCCCGTC CGGTCCAGAA
    GATCTAAACA AACCTGAACC CTGCACTCCT CCATCTCCTC TGCCTCCTGA AAGTCCACTG
    ACAGACCCCA TCCAGAGTGA GGAGGAGGAG TGGAGAACTG AGGAAAGCTC AGAAGCACAG
    ACCTTTAATC ACAACTCGGA CTCTGACCAC AATCTACAGC TGAAGGTGAG TCAGAGTGTG
    CAGAGCTTGA CTCAGCTGCT GTACAGTCTA CAGGCTGCAG TTACCATCCA AGACAGTTGC
    TATGAAGTGC AGAAGCTTCT TCTCCTGCTG ATGTCAAGCC CCGCCCCTAA CCAACAGTCC
    CGCCCACTAT CCATCACCCG CAGCGGGCAG GCTCTGCATG AGCAGGAGCG CCAGCGGGAG
    GCCGAGCGCC GCCGGGAAGA GCTGGCGGAT GTGGCTCGTC TGCGGGGAGC CCTGGTACGA
    GAGCGCCAGC AGTGGGAGAG GGAGTGCCAG GCGCGGCAGC TTCAGCACTC AGAACTGGAG
    ACCACGCTTG AGCAGAGGGA GCTCTTGTGC CACCTAGAGG AGCGGAGGCT GCAGAGCGAG
    CGGCAGGAGC TCCAGGAGCA GCTGCAGGAA TACCAACAGA GTCTGGAGAG GCTCAGAGAA
    GGCCAGAGGA ATGTGGAGAA GGAGAAGGAG AAACTGGAGA CTCAGAAGAT GCTGCTGGAG
    GGCTTGAAGC ACGGGTGCCA GCAGAACCTT CCAGCTATGG TCATACCACT GGACGGACAG
    CAGACACAGA ATCACCTGGG CATTCAGGAC GAGCATGGCT CTATTTTTGT CAATGAGGCA
    GCGTTCATAT CTTCTTCTGT AAATAACAGG CACCTCCATC TTCAGCATCA TCCACAGCAT
    CATCACCAGA CTGTCCATGT GCACTCAGGA TCTATAGGAT ACTCAGATTC TCCCAGCGCT
    CAGAACAGTC TTAATTCACT GCTAGCCCGA TCCAACCACA GACCCAGCTC TGCTGGAGCA
    TCCGACCACA GCTGGACGTC TCTGAGGAGT CTGACAGAGA GTGCAAACAA CAATGTGGGT
    CAGATCAGCC ATGTTGAAGA GTCTTGGTCA GCAGGGGTAA CGGGAGCGGG ACTCCATCAA
    ACTGATGTCC TGCTCGTCAG CCAGCCCTTC CTAAGCATGG AGACAGAGAA CAGAGATACT
    GATGGAGAGG AGACCATCGT GTATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332205.1
    CDS206..5635
    Translation

    Target ORF information:

    RefSeq Version XM_021476530.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GCCCGAGTCT GGTTGCCTTG
    GAAATGGACA GCGAGGAGGA TGACGATGAT GACATTTTAA TGAAGAAGCC CCTCCCACAA
    CTGCAACCGG ATTCCAAAGA CAGCACAGAT GAGCAGGTTA TGTCCAGCCC CGCACCTGTA
    CTCATCTGCA CGCGCTCTCA CTCTGCCTCC TCTGCCTGTA ACCCCGCCCC TGCAAAGGAT
    GTTGAGCAGG GTGTCCGACT CCGATCCTAT TCCTACACCT CCCCCAAAAT CAGCCTTCTG
    CCTCCACGTT TCAGCCGAGA CGCCCATCCT CCACCCGCCG ACCTCAGCCA GGATGGCGTT
    CTCTCCAGCA GCAGTGGCAG CAAGTCTCTT CTACACGCCC TGTCTCTGTC TAAATCTCTG
    TCTCTGTTCA ACCCTGTTAA GCACCGCTCT TCCAGCACAT CAGAACAGCC TCAAGAGAAA
    AGGGAGTTAA GGTTTCGCAG GCGAGCTCAG TCGGCTGATG ATGAAAGCAG TGCAGAGCTG
    GCCGACTCGC TCCAACATCT CACGCTCTCA GAGTTCCTCA AAGAGATTGA GGAAGAAGAA
    TGGGATAAAT ATATCATCCC TTCCAAAACA GAGTCAGAAA AGTACAAAGT CAGCCGCACA
    TTCAGCTTCA TAAAGAGCCG AATGTATAGC ACTCGCAACA AGAACAAGGG GAAAGGAAAA
    GAGAAAGAGA CCAAAGACAA GCAGGTCAAC GGACACCAGT TCACAGCGGG ATCTGTCCTG
    GGGTCGACAC TCTGTGAGAT CTGCAGCAAG CCAGCTTCAG GGAAAGAGGT TGTCCATTGC
    ACACACTGTG CAGTGAGTGT CCATAAGGGC TGTAAGGACT CATCTACGCC ATGCCTGAAG
    AAACCTCAAG ACAAATATTC AGTGATGACA GTAAAAAGCA AGACAGCCTC TCTTCCTCAA
    AATTTTATTG TGCGTGAGAG TTCATCGTCG TCCCTTCAGA TCTCTACCTC TGCTTCTCTC
    CCTGTGATGA CATCACGAGA CTCGGCTCCT CTTCCTTGTC CTCTGTCCAG AAGTGTGCCC
    TTAACCACTG AAAGGCTTAG CGAGAGCCCA GAAGCTGATA GTGATTCTAA TCTTTGGAGG
    AACCACATCC AATCAGAGGA GCTTCTCCAA ACCATGGAGT CCTCAACATC CACAGACTCG
    TCCATCATTG AAGACTCGGT TGATTCCCCT TTACAGATTG AGCTGCTGAT GAATGCTGTA
    GATTTGGAGG CAGAGTCATG GAGTTTAGCT GTGGAGGCCC AGTTCTGTAG GATGCAAGAA
    AAACGCATCA TCAAGAGACA GGATGTGATT TACGAGTTCA TGCAGACCGA GCTCCATCAC
    GTGCAGACAC TCATGGTGAT GGCAGAGGTC TTCAGAAGGG GAATGCGGGA TGAAGTGGGT
    CTGGACGCAG AGACCATTGG CCGGATTTTT CCTTGCTTAG ATGAGCTACT CCAACTCCAT
    CGTGATTTTC TGACAGCAAT GAGGGAACGA AGGCAAAGCT GCGTCCAGAA CAACAGCAGC
    AAAAACTTTC TCATCCACCG TGTGGGTGAT ATTTTTCTGC AACAGTTCTC CCAGGAGAAC
    GCAGAGAAGA TGAAGCAGGT GTATGGAGAG TTTTGCAGTC ATCACACAGA AGCAGTCAGT
    TACTTCAAAG AGCTTCAGCA GCAGAACAAG AGATTTCAGC TCTTCATCAA ACAACAGAGT
    AGTAACTCGC TGGTGAAGAG GCGAGAGATC CCAGAATGTA TTTTGTTAGT GACGCAGCGA
    ATTACAAAGT ACCCTGTCCT CCTAGAGAGA ATACTCCAGT ACACAGACGA GGGCACAGAA
    GAGCACTCTG ACCTCTCTCG AGCTCTAGTC CTGATCCGAG AGCTGATCTC CGCTGTGGAC
    CAATATGTGA GTCAGTATGA GCAGAACCAG AAGCTGAGTG AAGTCTTGGG CCGCATGGAG
    AACAAGTGCT CTACCAAACT GAAGACCGGC TGCACTTTCC GCAAGCAGGA CATCACCAGC
    GGCAGGAATC TGCTGCACCA GGGCCCGCTG CTCTGGAAGA CAGCCACTGG AAGACTGAAA
    GATGTTCTGG CATTTTTGCT GTCTGATTCA CTGGTGTTCC TTCAGGAGAA AGATCAGAAA
    TACATTTTTG CTACTGTGGA CCAGAAGCCT CCAGTGATCT CTTTGCAGAA ACTAATTGTG
    CGTGAGGTGG CCAATGAAGA GAGAGGCATG TTTCTGATCA GTGCATCTTC AGCCGGGCCT
    GAGATGTACG AGGTGCATGC AGCCTCTAAA GATGAACGCA ACGCCTGGAT GAGACTCATC
    AGAGAGGCCG TGGAGAATTG CCCTGAAGAG GAGGATGAAA CTACAAGTGA GTCAGAAGAG
    GAAAGGAGAG TCACAGAAGC AAAGGTCCAA AAGATCCACC GGCTGCAAGA GACGCTGTGT
    GGCCATGATC ACCTCATCTG TTCCAATCTG GAGGAGAAGC TGCACATCTA TGCCGAGCTG
    TCAGTGATGG CAGGCCAGCG AGAGGCAGGA CCAGAGCCCA GGCTGCTGGT ACGACCCAGC
    ACTGAAGACG TTCCCCAGGC TGGAGTACTA CTCAGCGCCG CTCTCAGAGA GGCGGAGAGC
    CTAAGAGCAG CTCTCTCCTC TCATTGTGCT TCCACTCCTC CCGTCCCGTC CGGTCCAGAA
    GATCTAAACA AACCTGAACC CTGCACTCCT CCATCTCCTC TGCCTCCTGA AAGTCCACTG
    ACAGACCCCA TCCAGAGTGA GGAGGAGGAG TGGAGAACTG AGGAAAGCTC AGAAGCACAG
    ACCTTTAATC ACAACTCGGA CTCTGACCAC AATCTACAGC TGAAGGTGAG TCAGAGTGTG
    CAGAGCTTGA CTCAGCTGCT GTACAGTCTA CAGGCTGCAG TTACCATCCA AGACAGTTGC
    TATGAAGTGC AGAAGCTTCT TCTCCTGCTG ATGTCAAGCC CCGCCCCTAA CCAACAGTCC
    CGCCCACTAT CCATCACCCG CAGCGGGCAG GCTCTGCATG AGCAGGAGCG CCAGCGGGAG
    GCCGAGCGCC GCCGGGAAGA GCTGGCGGAT GTGGCTCGTC TGCGGGGAGC CCTGGTACGA
    GAGCGCCAGC AGTGGGAGAG GGAGTGCCAG GCGCGGCAGC TTCAGCACTC AGAACTGGAG
    ACCACGCTTG AGCAGAGGGA GCTCTTGTGC CACCTAGAGG AGCGGAGGCT GCAGAGCGAG
    CGGCAGGAGC TCCAGGAGCA GCTGCAGGAA TACCAACAGA GTCTGGAGAG GCTCAGAGAA
    GGCCAGAGGA ATGTGGAGAA GGAGAAGGAG AAACTGGAGA CTCAGAAGAT GCTGCTGGAG
    GGCTTGAAGC ACGGGTGCCA GCAGAACCTT CCAGCTATGG TCATACCACT GGACGGACAG
    CAGACACAGA ATCACCTGGG CATTCAGGAC GAGCATGGCT CTATTTTTGT CAATGAGGCA
    GCGTTCATAT CTTCTTCTGT AAATAACAGG CACCTCCATC TTCAGCATCA TCCACAGCAT
    CATCACCAGA CTGTCCATGT GCACTCAGGA TCTATAGGAT ACTCAGATTC TCCCAGCGCT
    CAGAACAGTC TTAATTCACT GCTAGCCCGA TCCAACCACA GACCCAGCTC TGCTGGAGCA
    TCCGACCACA GCTGGACGTC TCTGAGGAGT CTGACAGAGA GTGCAAACAA CAATGTGGGT
    CAGATCAGCC ATGTTGAAGA GTCTTGGTCA GCAGGGGTAA CGGGAGCGGG ACTCCATCAA
    ACTGATGTCC TGCTCGTCAG CCAGCCCTTC CTAAGCATGG AGACAGAGAA CAGAGATACT
    GATGGAGAGG AGACCATCGT GTATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43200
    Clone ID Related Accession (Same CDS sequence) XM_021476530.1 , XM_009301598.2
    Accession Version XM_009301598.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GCCCGAGTCT GGTTGCCTTG
    GAAATGGACA GCGAGGAGGA TGACGATGAT GACATTTTAA TGAAGAAGCC CCTCCCACAA
    CTGCAACCGG ATTCCAAAGA CAGCACAGAT GAGCAGGTTA TGTCCAGCCC CGCACCTGTA
    CTCATCTGCA CGCGCTCTCA CTCTGCCTCC TCTGCCTGTA ACCCCGCCCC TGCAAAGGAT
    GTTGAGCAGG GTGTCCGACT CCGATCCTAT TCCTACACCT CCCCCAAAAT CAGCCTTCTG
    CCTCCACGTT TCAGCCGAGA CGCCCATCCT CCACCCGCCG ACCTCAGCCA GGATGGCGTT
    CTCTCCAGCA GCAGTGGCAG CAAGTCTCTT CTACACGCCC TGTCTCTGTC TAAATCTCTG
    TCTCTGTTCA ACCCTGTTAA GCACCGCTCT TCCAGCACAT CAGAACAGCC TCAAGAGAAA
    AGGGAGTTAA GGTTTCGCAG GCGAGCTCAG TCGGCTGATG ATGAAAGCAG TGCAGAGCTG
    GCCGACTCGC TCCAACATCT CACGCTCTCA GAGTTCCTCA AAGAGATTGA GGAAGAAGAA
    TGGGATAAAT ATATCATCCC TTCCAAAACA GAGTCAGAAA AGTACAAAGT CAGCCGCACA
    TTCAGCTTCA TAAAGAGCCG AATGTATAGC ACTCGCAACA AGAACAAGGG GAAAGGAAAA
    GAGAAAGAGA CCAAAGACAA GCAGGTCAAC GGACACCAGT TCACAGCGGG ATCTGTCCTG
    GGGTCGACAC TCTGTGAGAT CTGCAGCAAG CCAGCTTCAG GGAAAGAGGT TGTCCATTGC
    ACACACTGTG CAGTGAGTGT CCATAAGGGC TGTAAGGACT CATCTACGCC ATGCCTGAAG
    AAACCTCAAG ACAAATATTC AGTGATGACA GTAAAAAGCA AGACAGCCTC TCTTCCTCAA
    AATTTTATTG TGCGTGAGAG TTCATCGTCG TCCCTTCAGA TCTCTACCTC TGCTTCTCTC
    CCTGTGATGA CATCACGAGA CTCGGCTCCT CTTCCTTGTC CTCTGTCCAG AAGTGTGCCC
    TTAACCACTG AAAGGCTTAG CGAGAGCCCA GAAGCTGATA GTGATTCTAA TCTTTGGAGG
    AACCACATCC AATCAGAGGA GCTTCTCCAA ACCATGGAGT CCTCAACATC CACAGACTCG
    TCCATCATTG AAGACTCGGT TGATTCCCCT TTACAGATTG AGCTGCTGAT GAATGCTGTA
    GATTTGGAGG CAGAGTCATG GAGTTTAGCT GTGGAGGCCC AGTTCTGTAG GATGCAAGAA
    AAACGCATCA TCAAGAGACA GGATGTGATT TACGAGTTCA TGCAGACCGA GCTCCATCAC
    GTGCAGACAC TCATGGTGAT GGCAGAGGTC TTCAGAAGGG GAATGCGGGA TGAAGTGGGT
    CTGGACGCAG AGACCATTGG CCGGATTTTT CCTTGCTTAG ATGAGCTACT CCAACTCCAT
    CGTGATTTTC TGACAGCAAT GAGGGAACGA AGGCAAAGCT GCGTCCAGAA CAACAGCAGC
    AAAAACTTTC TCATCCACCG TGTGGGTGAT ATTTTTCTGC AACAGTTCTC CCAGGAGAAC
    GCAGAGAAGA TGAAGCAGGT GTATGGAGAG TTTTGCAGTC ATCACACAGA AGCAGTCAGT
    TACTTCAAAG AGCTTCAGCA GCAGAACAAG AGATTTCAGC TCTTCATCAA ACAACAGAGT
    AGTAACTCGC TGGTGAAGAG GCGAGAGATC CCAGAATGTA TTTTGTTAGT GACGCAGCGA
    ATTACAAAGT ACCCTGTCCT CCTAGAGAGA ATACTCCAGT ACACAGACGA GGGCACAGAA
    GAGCACTCTG ACCTCTCTCG AGCTCTAGTC CTGATCCGAG AGCTGATCTC CGCTGTGGAC
    CAATATGTGA GTCAGTATGA GCAGAACCAG AAGCTGAGTG AAGTCTTGGG CCGCATGGAG
    AACAAGTGCT CTACCAAACT GAAGACCGGC TGCACTTTCC GCAAGCAGGA CATCACCAGC
    GGCAGGAATC TGCTGCACCA GGGCCCGCTG CTCTGGAAGA CAGCCACTGG AAGACTGAAA
    GATGTTCTGG CATTTTTGCT GTCTGATTCA CTGGTGTTCC TTCAGGAGAA AGATCAGAAA
    TACATTTTTG CTACTGTGGA CCAGAAGCCT CCAGTGATCT CTTTGCAGAA ACTAATTGTG
    CGTGAGGTGG CCAATGAAGA GAGAGGCATG TTTCTGATCA GTGCATCTTC AGCCGGGCCT
    GAGATGTACG AGGTGCATGC AGCCTCTAAA GATGAACGCA ACGCCTGGAT GAGACTCATC
    AGAGAGGCCG TGGAGAATTG CCCTGAAGAG GAGGATGAAA CTACAAGTGA GTCAGAAGAG
    GAAAGGAGAG TCACAGAAGC AAAGGTCCAA AAGATCCACC GGCTGCAAGA GACGCTGTGT
    GGCCATGATC ACCTCATCTG TTCCAATCTG GAGGAGAAGC TGCACATCTA TGCCGAGCTG
    TCAGTGATGG CAGGCCAGCG AGAGGCAGGA CCAGAGCCCA GGCTGCTGGT ACGACCCAGC
    ACTGAAGACG TTCCCCAGGC TGGAGTACTA CTCAGCGCCG CTCTCAGAGA GGCGGAGAGC
    CTAAGAGCAG CTCTCTCCTC TCATTGTGCT TCCACTCCTC CCGTCCCGTC CGGTCCAGAA
    GATCTAAACA AACCTGAACC CTGCACTCCT CCATCTCCTC TGCCTCCTGA AAGTCCACTG
    ACAGACCCCA TCCAGAGTGA GGAGGAGGAG TGGAGAACTG AGGAAAGCTC AGAAGCACAG
    ACCTTTAATC ACAACTCGGA CTCTGACCAC AATCTACAGC TGAAGGTGAG TCAGAGTGTG
    CAGAGCTTGA CTCAGCTGCT GTACAGTCTA CAGGCTGCAG TTACCATCCA AGACAGTTGC
    TATGAAGTGC AGAAGCTTCT TCTCCTGCTG ATGTCAAGCC CCGCCCCTAA CCAACAGTCC
    CGCCCACTAT CCATCACCCG CAGCGGGCAG GCTCTGCATG AGCAGGAGCG CCAGCGGGAG
    GCCGAGCGCC GCCGGGAAGA GCTGGCGGAT GTGGCTCGTC TGCGGGGAGC CCTGGTACGA
    GAGCGCCAGC AGTGGGAGAG GGAGTGCCAG GCGCGGCAGC TTCAGCACTC AGAACTGGAG
    ACCACGCTTG AGCAGAGGGA GCTCTTGTGC CACCTAGAGG AGCGGAGGCT GCAGAGCGAG
    CGGCAGGAGC TCCAGGAGCA GCTGCAGGAA TACCAACAGA GTCTGGAGAG GCTCAGAGAA
    GGCCAGAGGA ATGTGGAGAA GGAGAAGGAG AAACTGGAGA CTCAGAAGAT GCTGCTGGAG
    GGCTTGAAGC ACGGGTGCCA GCAGAACCTT CCAGCTATGG TCATACCACT GGACGGACAG
    CAGACACAGA ATCACCTGGG CATTCAGGAC GAGCATGGCT CTATTTTTGT CAATGAGGCA
    GCGTTCATAT CTTCTTCTGT AAATAACAGG CACCTCCATC TTCAGCATCA TCCACAGCAT
    CATCACCAGA CTGTCCATGT GCACTCAGGA TCTATAGGAT ACTCAGATTC TCCCAGCGCT
    CAGAACAGTC TTAATTCACT GCTAGCCCGA TCCAACCACA GACCCAGCTC TGCTGGAGCA
    TCCGACCACA GCTGGACGTC TCTGAGGAGT CTGACAGAGA GTGCAAACAA CAATGTGGGT
    CAGATCAGCC ATGTTGAAGA GTCTTGGTCA GCAGGGGTAA CGGGAGCGGG ACTCCATCAA
    ACTGATGTCC TGCTCGTCAG CCAGCCCTTC CTAAGCATGG AGACAGAGAA CAGAGATACT
    GATGGAGAGG AGACCATCGT GTATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299873.1
    CDS197..5626
    Translation

    Target ORF information:

    RefSeq Version XM_009301598.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 28 (arhgef28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301598.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCTCA GCAGGGGAGA GGTGCCACTT TATGGTCAGG TGGAGGTCTG CGTGAAGCTG 
    CCGCTGTCTG AGTTTGTTCA GGAGGAAACG GACTACTATG TGCTGCTTGT GGGCTCTTCC
    CTTCACCATG TAACTGCAGC TCATAGGACT GAGGATCACA GCTCCATTAG GTTCACTGTA
    CCTGGTCACA TTGTTCCAGA AAAGGTGTCC GTGGAGGTCT ACAGCTGTAC AAAGGAGAGC
    AAAAGTCCCA TCAGCCCCTT CTGCCATTCA GAAAAGCTCT CTGTGGAGTA CATTTTAGAT
    GAGGTACAAG AAGTGGCAGA GCAGCTGATG AACTTTGAAA CCTTGACATC CAGCAGCCAT
    CAGGACATTC TAAAGAAATT ATACCCAAAG AAAAAGGTTT TCTCTGAGAA AAACCATGTA
    GAACAAACTG CCCAGCATGG GACAGATGGT GTTTGTGATC CACATGGGGG ACTGCTTCAG
    TTGGAAAGCC TTGCAGAGAC AGAACAAGGG TTCAATGTTT GCGAACAGGG GAATTTAAGC
    CAGAGTGAAG TTTCAGGAAC GGAGGAGTTT GATCCAGAGC AACACAGTTC TGTTGATGAA
    AAAGTCACAC AGGCCTTGGC CAACTTGCAC TTTTTGGGAT GGGATGGCCA GGCTGGACAG
    GAGAACCAAG TTCTTCTTCC CAGGGAAACT CCTCTCCATG TGGCCATGCG TTTAGGCTTC
    CCCCACCTCA GTCACTTCCT CCTCCGAGAG TTTAATGGTC AGATGATGGT TTCCATTGCC
    AATGAGGAAG GACATACTCC TGTAGCACTT GCTGAGAAAC ATGGAGATCC TGTGCTCATC
    ACTGCTTTAA CACATGGCAG CGCACTGAGG CCAGTATGGA TGTGTCAGAT ATGGACTGAC
    TGCTCTCAAC AGCTCAGAAT CTGCCCCGTC ACCGACTCCA TCAGTCTGAC TGTCAGACAC
    CAGACTCTGA TAAATGTACA AAACAGCATC CAGCTATACA GAGAGAAGAG CAAAGAGCCG
    GACATACTCA CACGGGTGCG GCATCTGAGT GAGCTGTCTG AGGGGAGCGT GCACAGATCT
    GATGAATGTG ATGCCGACCT GAACAACTCT CTCAAAATCG AGGACCAGAC ATTGGTTGAT
    AGTGTCTTTG AAGAACAGCT TGTGCTCTGT CTGGATGAGG AGGATGAAGA TCTAAGCGTG
    TTTAATTCAG AGAAGCAGTT TTCGTCTCCG GGCTCCTGCA CTCCGTTTCA CCTTCATTCC
    TCAGCCGCTG CAGCCGTCAG GCTCACTGCC ATGCTCAAAG GCTCCACTAA GGACAGTGAG
    CTGAATATGA AGTACGCCGT TCCAGGTGCC ACAGAGGACA GCTCTGGGAC CGATAGTGGC
    ATGTGGCGGA TGTCAGATAC GGGGGTCTCA TCACCAGGGA CAATAGATAT ATCTCCAACT
    GTTAAAGATG ATGAAGGGCA GAGAGGACGG TACCCCGCCT CTCGTTCTTT CTCTCCCACC
    TCTCCAGCAG GACTGATGCT GGATAGATTC ATGCGGGCCA CGAGAACCAC TGAACTGACC
    TACAACAGCT CAAGCTCACC TACAGAGTCA TGTGACCTCA GCCCGAGTCT GGTTGCCTTG
    GAAATGGACA GCGAGGAGGA TGACGATGAT GACATTTTAA TGAAGAAGCC CCTCCCACAA
    CTGCAACCGG ATTCCAAAGA CAGCACAGAT GAGCAGGTTA TGTCCAGCCC CGCACCTGTA
    CTCATCTGCA CGCGCTCTCA CTCTGCCTCC TCTGCCTGTA ACCCCGCCCC TGCAAAGGAT
    GTTGAGCAGG GTGTCCGACT CCGATCCTAT TCCTACACCT CCCCCAAAAT CAGCCTTCTG
    CCTCCACGTT TCAGCCGAGA CGCCCATCCT CCACCCGCCG ACCTCAGCCA GGATGGCGTT
    CTCTCCAGCA GCAGTGGCAG CAAGTCTCTT CTACACGCCC TGTCTCTGTC TAAATCTCTG
    TCTCTGTTCA ACCCTGTTAA GCACCGCTCT TCCAGCACAT CAGAACAGCC TCAAGAGAAA
    AGGGAGTTAA GGTTTCGCAG GCGAGCTCAG TCGGCTGATG ATGAAAGCAG TGCAGAGCTG
    GCCGACTCGC TCCAACATCT CACGCTCTCA GAGTTCCTCA AAGAGATTGA GGAAGAAGAA
    TGGGATAAAT ATATCATCCC TTCCAAAACA GAGTCAGAAA AGTACAAAGT CAGCCGCACA
    TTCAGCTTCA TAAAGAGCCG AATGTATAGC ACTCGCAACA AGAACAAGGG GAAAGGAAAA
    GAGAAAGAGA CCAAAGACAA GCAGGTCAAC GGACACCAGT TCACAGCGGG ATCTGTCCTG
    GGGTCGACAC TCTGTGAGAT CTGCAGCAAG CCAGCTTCAG GGAAAGAGGT TGTCCATTGC
    ACACACTGTG CAGTGAGTGT CCATAAGGGC TGTAAGGACT CATCTACGCC ATGCCTGAAG
    AAACCTCAAG ACAAATATTC AGTGATGACA GTAAAAAGCA AGACAGCCTC TCTTCCTCAA
    AATTTTATTG TGCGTGAGAG TTCATCGTCG TCCCTTCAGA TCTCTACCTC TGCTTCTCTC
    CCTGTGATGA CATCACGAGA CTCGGCTCCT CTTCCTTGTC CTCTGTCCAG AAGTGTGCCC
    TTAACCACTG AAAGGCTTAG CGAGAGCCCA GAAGCTGATA GTGATTCTAA TCTTTGGAGG
    AACCACATCC AATCAGAGGA GCTTCTCCAA ACCATGGAGT CCTCAACATC CACAGACTCG
    TCCATCATTG AAGACTCGGT TGATTCCCCT TTACAGATTG AGCTGCTGAT GAATGCTGTA
    GATTTGGAGG CAGAGTCATG GAGTTTAGCT GTGGAGGCCC AGTTCTGTAG GATGCAAGAA
    AAACGCATCA TCAAGAGACA GGATGTGATT TACGAGTTCA TGCAGACCGA GCTCCATCAC
    GTGCAGACAC TCATGGTGAT GGCAGAGGTC TTCAGAAGGG GAATGCGGGA TGAAGTGGGT
    CTGGACGCAG AGACCATTGG CCGGATTTTT CCTTGCTTAG ATGAGCTACT CCAACTCCAT
    CGTGATTTTC TGACAGCAAT GAGGGAACGA AGGCAAAGCT GCGTCCAGAA CAACAGCAGC
    AAAAACTTTC TCATCCACCG TGTGGGTGAT ATTTTTCTGC AACAGTTCTC CCAGGAGAAC
    GCAGAGAAGA TGAAGCAGGT GTATGGAGAG TTTTGCAGTC ATCACACAGA AGCAGTCAGT
    TACTTCAAAG AGCTTCAGCA GCAGAACAAG AGATTTCAGC TCTTCATCAA ACAACAGAGT
    AGTAACTCGC TGGTGAAGAG GCGAGAGATC CCAGAATGTA TTTTGTTAGT GACGCAGCGA
    ATTACAAAGT ACCCTGTCCT CCTAGAGAGA ATACTCCAGT ACACAGACGA GGGCACAGAA
    GAGCACTCTG ACCTCTCTCG AGCTCTAGTC CTGATCCGAG AGCTGATCTC CGCTGTGGAC
    CAATATGTGA GTCAGTATGA GCAGAACCAG AAGCTGAGTG AAGTCTTGGG CCGCATGGAG
    AACAAGTGCT CTACCAAACT GAAGACCGGC TGCACTTTCC GCAAGCAGGA CATCACCAGC
    GGCAGGAATC TGCTGCACCA GGGCCCGCTG CTCTGGAAGA CAGCCACTGG AAGACTGAAA
    GATGTTCTGG CATTTTTGCT GTCTGATTCA CTGGTGTTCC TTCAGGAGAA AGATCAGAAA
    TACATTTTTG CTACTGTGGA CCAGAAGCCT CCAGTGATCT CTTTGCAGAA ACTAATTGTG
    CGTGAGGTGG CCAATGAAGA GAGAGGCATG TTTCTGATCA GTGCATCTTC AGCCGGGCCT
    GAGATGTACG AGGTGCATGC AGCCTCTAAA GATGAACGCA ACGCCTGGAT GAGACTCATC
    AGAGAGGCCG TGGAGAATTG CCCTGAAGAG GAGGATGAAA CTACAAGTGA GTCAGAAGAG
    GAAAGGAGAG TCACAGAAGC AAAGGTCCAA AAGATCCACC GGCTGCAAGA GACGCTGTGT
    GGCCATGATC ACCTCATCTG TTCCAATCTG GAGGAGAAGC TGCACATCTA TGCCGAGCTG
    TCAGTGATGG CAGGCCAGCG AGAGGCAGGA CCAGAGCCCA GGCTGCTGGT ACGACCCAGC
    ACTGAAGACG TTCCCCAGGC TGGAGTACTA CTCAGCGCCG CTCTCAGAGA GGCGGAGAGC
    CTAAGAGCAG CTCTCTCCTC TCATTGTGCT TCCACTCCTC CCGTCCCGTC CGGTCCAGAA
    GATCTAAACA AACCTGAACC CTGCACTCCT CCATCTCCTC TGCCTCCTGA AAGTCCACTG
    ACAGACCCCA TCCAGAGTGA GGAGGAGGAG TGGAGAACTG AGGAAAGCTC AGAAGCACAG
    ACCTTTAATC ACAACTCGGA CTCTGACCAC AATCTACAGC TGAAGGTGAG TCAGAGTGTG
    CAGAGCTTGA CTCAGCTGCT GTACAGTCTA CAGGCTGCAG TTACCATCCA AGACAGTTGC
    TATGAAGTGC AGAAGCTTCT TCTCCTGCTG ATGTCAAGCC CCGCCCCTAA CCAACAGTCC
    CGCCCACTAT CCATCACCCG CAGCGGGCAG GCTCTGCATG AGCAGGAGCG CCAGCGGGAG
    GCCGAGCGCC GCCGGGAAGA GCTGGCGGAT GTGGCTCGTC TGCGGGGAGC CCTGGTACGA
    GAGCGCCAGC AGTGGGAGAG GGAGTGCCAG GCGCGGCAGC TTCAGCACTC AGAACTGGAG
    ACCACGCTTG AGCAGAGGGA GCTCTTGTGC CACCTAGAGG AGCGGAGGCT GCAGAGCGAG
    CGGCAGGAGC TCCAGGAGCA GCTGCAGGAA TACCAACAGA GTCTGGAGAG GCTCAGAGAA
    GGCCAGAGGA ATGTGGAGAA GGAGAAGGAG AAACTGGAGA CTCAGAAGAT GCTGCTGGAG
    GGCTTGAAGC ACGGGTGCCA GCAGAACCTT CCAGCTATGG TCATACCACT GGACGGACAG
    CAGACACAGA ATCACCTGGG CATTCAGGAC GAGCATGGCT CTATTTTTGT CAATGAGGCA
    GCGTTCATAT CTTCTTCTGT AAATAACAGG CACCTCCATC TTCAGCATCA TCCACAGCAT
    CATCACCAGA CTGTCCATGT GCACTCAGGA TCTATAGGAT ACTCAGATTC TCCCAGCGCT
    CAGAACAGTC TTAATTCACT GCTAGCCCGA TCCAACCACA GACCCAGCTC TGCTGGAGCA
    TCCGACCACA GCTGGACGTC TCTGAGGAGT CTGACAGAGA GTGCAAACAA CAATGTGGGT
    CAGATCAGCC ATGTTGAAGA GTCTTGGTCA GCAGGGGTAA CGGGAGCGGG ACTCCATCAA
    ACTGATGTCC TGCTCGTCAG CCAGCCCTTC CTAAGCATGG AGACAGAGAA CAGAGATACT
    GATGGAGAGG AGACCATCGT GTATCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.