edem3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	edem3
Entrez Gene ID	559811
Full Name	ER degradation enhancer, mannosidase alpha-like 3
General protein information	Preferred Names ER degradation enhancer, mannosidase alpha-like 3 Names ER degradation-enhancing alpha-mannosidase-like 3 ER degradation-enhancing alpha-mannosidase-like protein 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166932.4	XP_005166989.2	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
XM_005166934.3	XP_005166991.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
XM_683183.7	XP_688275.5	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X4
XM_005166932.3	XP_005166989.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
XM_005166934.4	XP_005166991.2	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
XM_009303906.2	XP_009302181.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
XM_009303906.3	XP_009302181.2	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
XM_683183.8	XP_688275.6	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Edem3	NP_001034733.2
Rattus norvegicus (Norway rat)	Edem3	NP_001178600.1
Danio rerio (zebrafish)	edem3	XP_005166989.1
Pan troglodytes (chimpanzee)	EDEM3	XP_003308683.1
Caenorhabditis elegans (roundworm)	ZC506.1	NP_509671.3
Xenopus tropicalis (tropical clawed frog)	edem3	XP_002932190.2
Homo sapiens (human)	EDEM3	NP_079467.3
	EDEM3	XP_002802060.1
Canis lupus familiaris (dog)	EDEM3	XP_005622487.1
Bos taurus (cattle)	EDEM3	NP_001192782.1
Gallus gallus (chicken)	EDEM3	XP_422293.4
Drosophila melanogaster (fruit fly)	Edem2	NP_609611.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008749	XP_314865.3

Gene Ontology

The following edem3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the edem3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa14097	XM_005166932.3 Latest version!	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa14099	XM_005166934.3 Latest version!	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa14100	XM_683183.7 Latest version!	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa43176	XM_009303906.2 Latest version!	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa143777	XM_005166932.4 Latest version!	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa143778	XM_009303906.3 Latest version!	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa143779	XM_005166934.4 Latest version!	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa143780	XM_683183.8 Latest version!	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

edem3 ( XM_005166932.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166989.1 Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14097
Clone ID Related Accession (Same CDS sequence)	XM_005166932.3
Accession Version	XM_005166932.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTTTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCA 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AATGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT TCTTGAATTT 
GCCGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCCATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GAACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTCCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCACA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTAAGTCTC 
ATCCATCTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCGCAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGCCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGAAGCCATA 
TTTAAGGGAA AAGCCCTCCC GGGCGCCCTG TTGGATGGCA CTACAGATGG AGCAGAAGAA 
GAAGACTGTT CCACTGAGGA GAGAGACCAC AGTTCACCCG CCCCCTCAGA ACCCGTTGAC 
CAATCACAAG TGACCACTCA GGAACAGACA GCAGGCGACT CCGAGTCAGT TCAGCCTGCG 
GAGGGACTGG GCAGCTACGT CTCCGTGGAT GCAGGGGAGG AGCCTCCAGC AGACGAAGAC 
TCCAACAACC AATCGGTGGA CTCGCTGATG GCTGATTGGC AGGAGGACTT GGAAGCATTT 
AAGCAAATGG AGAAGGATGA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166989.1
CDS	1112..3778
Translation	MWMLHSATMLPAFWLLFFMGTCIRLSETMSRDEKSKLRIQVLEMFDHAYRNYMDHAYPADELMPLTCRGRVRGLEPSRGDVDDALGKFSLTLIDTLDTLALLNKTVEFEEAVRRVVKDVRLDNDIVVSVFETNIRVLGGLLGGHSMAVMLKENGQMVWYQNELLHMAKDLGLRLLPAFNTSSGLPYPRVNLRHGVHGPETRTGTETETCTACAGTIILEFAALSRFTGDPTFEAHARRALDFLWEKRQRNSNLVGTTINIHSGEWVRRDSGVGAGIDSYYEYLMKAYILLGDDQLLLRFNTHYASIMKYISQPPLLLDVHIHKPLLPARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVTKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATRDPYYLEVGRTVLENLNRFARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLFLLFAEEEDLPFNVEDYVFTTEAHLLPLSLSTASNSPAPPVINSVVIEELDDLNFEWTCPNTRLLFPDPAFPRNFREPIRSAVDKSCLRPIAHREPGMGRPPLRAQDFMANNPEHLELLRRMGVSLIHLKDGRVQLVQHATQAVSAVAAEDGMRFMQEMMELSSQQQKEQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKSSSGVRGFVTVAEPYNGCSELSNGEIVAGRIALLQRGQCMFAEKARHVQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGRNTDDVTLPLLFLFHKEANILLEALKEYKEVEVLLSDKARDREAIFKGKALPGALLDGTTDGAEEEDCSTEERDHSSPAPSEPVDQSQVTTQEQTAGDSESVQPAEGLGSYVSVDAGEEPPADEDSNNQSVDSLMADWQEDLEAFKQMEKDEL

Target ORF information:

RefSeq Version	XM_005166932.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166932.3

ORF Insert Sequence:

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTTTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCA 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AATGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT TCTTGAATTT 
GCCGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCCATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GAACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTCCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCACA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTAAGTCTC 
ATCCATCTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCGCAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGCCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGAAGCCATA 
TTTAAGGGAA AAGCCCTCCC GGGCGCCCTG TTGGATGGCA CTACAGATGG AGCAGAAGAA 
GAAGACTGTT CCACTGAGGA GAGAGACCAC AGTTCACCCG CCCCCTCAGA ACCCGTTGAC 
CAATCACAAG TGACCACTCA GGAACAGACA GCAGGCGACT CCGAGTCAGT TCAGCCTGCG 
GAGGGACTGG GCAGCTACGT CTCCGTGGAT GCAGGGGAGG AGCCTCCAGC AGACGAAGAC 
TCCAACAACC AATCGGTGGA CTCGCTGATG GCTGATTGGC AGGAGGACTT GGAAGCATTT 
AAGCAAATGG AGAAGGATGA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edem3 ( XM_005166934.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166991.1 Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa14099
Clone ID Related Accession (Same CDS sequence)	XM_005166934.3
Accession Version	XM_005166934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTTTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCA 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AATGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT TCTTGAATTT 
GCCGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCCATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GAACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTCCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCACA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTAAGTCTC 
ATCCATCTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCGCAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGCCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGCTACAGAT 
GGAGCAGAAG AAGAAGACTG TTCCACTGAG GAGAGAGACC ACAGTTCACC CGCCCCCTCA 
GAACCCGTTG ACCAATCACA AGTGACCACT CAGGAACAGA CAGCAGGCGA CTCCGAGTCA 
GTTCAGCCTG CGGAGGGACT GGGCAGCTAC GTCTCCGTGG ATGCAGGGGA GGAGCCTCCA 
GCAGACGAAG ACTCCAACAA CCAATCGGTG GACTCGCTGA TGGCTGATTG GCAGGAGGAC 
TTGGAAGCAT TTAAGCAAAT GGAGAAGGAT GAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166991.1
CDS	1112..3730
Translation	MWMLHSATMLPAFWLLFFMGTCIRLSETMSRDEKSKLRIQVLEMFDHAYRNYMDHAYPADELMPLTCRGRVRGLEPSRGDVDDALGKFSLTLIDTLDTLALLNKTVEFEEAVRRVVKDVRLDNDIVVSVFETNIRVLGGLLGGHSMAVMLKENGQMVWYQNELLHMAKDLGLRLLPAFNTSSGLPYPRVNLRHGVHGPETRTGTETETCTACAGTIILEFAALSRFTGDPTFEAHARRALDFLWEKRQRNSNLVGTTINIHSGEWVRRDSGVGAGIDSYYEYLMKAYILLGDDQLLLRFNTHYASIMKYISQPPLLLDVHIHKPLLPARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVTKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATRDPYYLEVGRTVLENLNRFARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLFLLFAEEEDLPFNVEDYVFTTEAHLLPLSLSTASNSPAPPVINSVVIEELDDLNFEWTCPNTRLLFPDPAFPRNFREPIRSAVDKSCLRPIAHREPGMGRPPLRAQDFMANNPEHLELLRRMGVSLIHLKDGRVQLVQHATQAVSAVAAEDGMRFMQEMMELSSQQQKEQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKSSSGVRGFVTVAEPYNGCSELSNGEIVAGRIALLQRGQCMFAEKARHVQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGRNTDDVTLPLLFLFHKEANILLEALKEYKEVEVLLSDKARDRATDGAEEEDCSTEERDHSSPAPSEPVDQSQVTTQEQTAGDSESVQPAEGLGSYVSVDAGEEPPADEDSNNQSVDSLMADWQEDLEAFKQMEKDEL

Target ORF information:

RefSeq Version	XM_005166934.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166934.3

ORF Insert Sequence:

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTTTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCA 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AATGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT TCTTGAATTT 
GCCGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCCATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GAACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTCCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCACA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTAAGTCTC 
ATCCATCTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCGCAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGCCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGCTACAGAT 
GGAGCAGAAG AAGAAGACTG TTCCACTGAG GAGAGAGACC ACAGTTCACC CGCCCCCTCA 
GAACCCGTTG ACCAATCACA AGTGACCACT CAGGAACAGA CAGCAGGCGA CTCCGAGTCA 
GTTCAGCCTG CGGAGGGACT GGGCAGCTAC GTCTCCGTGG ATGCAGGGGA GGAGCCTCCA 
GCAGACGAAG ACTCCAACAA CCAATCGGTG GACTCGCTGA TGGCTGATTG GCAGGAGGAC 
TTGGAAGCAT TTAAGCAAAT GGAGAAGGAT GAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edem3 ( XM_683183.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_688275.5 Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa14100
Clone ID Related Accession (Same CDS sequence)	XM_683183.7
Accession Version	XM_683183.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_683183.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTTTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCA 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AATGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT TCTTGAATTT 
GCCGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCCATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GAACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTCCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCACA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTAAGTCTC 
ATCCATCTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCGCAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGCCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGATGGAGCA 
GAAGAAGAAG ACTGTTCCAC TGAGGAGAGA GACCACAGTT CACCCGCCCC CTCAGAACCC 
GTTGACCAAT CACAAGTGAC CACTCAGGAA CAGACAGCAG GCGACTCCGA GTCAGTTCAG 
CCTGCGGAGG GACTGGGCAG CTACGTCTCC GTGGATGCAG GGGAGGAGCC TCCAGCAGAC 
GAAGACTCCA ACAACCAATC GGTGGACTCG CTGATGGCTG ATTGGCAGGA GGACTTGGAA 
GCATTTAAGC AAATGGAGAA GGATGAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688275.5
CDS	1112..3724
Translation	MWMLHSATMLPAFWLLFFMGTCIRLSETMSRDEKSKLRIQVLEMFDHAYRNYMDHAYPADELMPLTCRGRVRGLEPSRGDVDDALGKFSLTLIDTLDTLALLNKTVEFEEAVRRVVKDVRLDNDIVVSVFETNIRVLGGLLGGHSMAVMLKENGQMVWYQNELLHMAKDLGLRLLPAFNTSSGLPYPRVNLRHGVHGPETRTGTETETCTACAGTIILEFAALSRFTGDPTFEAHARRALDFLWEKRQRNSNLVGTTINIHSGEWVRRDSGVGAGIDSYYEYLMKAYILLGDDQLLLRFNTHYASIMKYISQPPLLLDVHIHKPLLPARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVTKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATRDPYYLEVGRTVLENLNRFARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLFLLFAEEEDLPFNVEDYVFTTEAHLLPLSLSTASNSPAPPVINSVVIEELDDLNFEWTCPNTRLLFPDPAFPRNFREPIRSAVDKSCLRPIAHREPGMGRPPLRAQDFMANNPEHLELLRRMGVSLIHLKDGRVQLVQHATQAVSAVAAEDGMRFMQEMMELSSQQQKEQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKSSSGVRGFVTVAEPYNGCSELSNGEIVAGRIALLQRGQCMFAEKARHVQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGRNTDDVTLPLLFLFHKEANILLEALKEYKEVEVLLSDKARDRDGAEEEDCSTEERDHSSPAPSEPVDQSQVTTQEQTAGDSESVQPAEGLGSYVSVDAGEEPPADEDSNNQSVDSLMADWQEDLEAFKQMEKDEL

Target ORF information:

RefSeq Version	XM_683183.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683183.7

ORF Insert Sequence:

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTTTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCA 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AATGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT TCTTGAATTT 
GCCGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCCATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GAACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTCCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCACA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTAAGTCTC 
ATCCATCTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCGCAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGCCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGATGGAGCA 
GAAGAAGAAG ACTGTTCCAC TGAGGAGAGA GACCACAGTT CACCCGCCCC CTCAGAACCC 
GTTGACCAAT CACAAGTGAC CACTCAGGAA CAGACAGCAG GCGACTCCGA GTCAGTTCAG 
CCTGCGGAGG GACTGGGCAG CTACGTCTCC GTGGATGCAG GGGAGGAGCC TCCAGCAGAC 
GAAGACTCCA ACAACCAATC GGTGGACTCG CTGATGGCTG ATTGGCAGGA GGACTTGGAA 
GCATTTAAGC AAATGGAGAA GGATGAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edem3 ( XM_009303906.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302181.1 Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43176
Clone ID Related Accession (Same CDS sequence)	XM_009303906.2
Accession Version	XM_009303906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTTTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCA 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AATGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT TCTTGAATTT 
GCCGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCCATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GAACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTCCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCACA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTAAGTCTC 
ATCCATCTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCGCAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGCCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGAAGCCATA 
TTTAAGGGAA AAGCCCTCCC GGGCGCCCTG TTGGATGGCA ATGGAGCAGA AGAAGAAGAC 
TGTTCCACTG AGGAGAGAGA CCACAGTTCA CCCGCCCCCT CAGAACCCGT TGACCAATCA 
CAAGTGACCA CTCAGGAACA GACAGCAGGC GACTCCGAGT CAGTTCAGCC TGCGGAGGGA 
CTGGGCAGCT ACGTCTCCGT GGATGCAGGG GAGGAGCCTC CAGCAGACGA AGACTCCAAC 
AACCAATCGG TGGACTCGCT GATGGCTGAT TGGCAGGAGG ACTTGGAAGC ATTTAAGCAA 
ATGGAGAAGG ATGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302181.1
CDS	1112..3772
Translation	MWMLHSATMLPAFWLLFFMGTCIRLSETMSRDEKSKLRIQVLEMFDHAYRNYMDHAYPADELMPLTCRGRVRGLEPSRGDVDDALGKFSLTLIDTLDTLALLNKTVEFEEAVRRVVKDVRLDNDIVVSVFETNIRVLGGLLGGHSMAVMLKENGQMVWYQNELLHMAKDLGLRLLPAFNTSSGLPYPRVNLRHGVHGPETRTGTETETCTACAGTIILEFAALSRFTGDPTFEAHARRALDFLWEKRQRNSNLVGTTINIHSGEWVRRDSGVGAGIDSYYEYLMKAYILLGDDQLLLRFNTHYASIMKYISQPPLLLDVHIHKPLLPARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVTKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATRDPYYLEVGRTVLENLNRFARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLFLLFAEEEDLPFNVEDYVFTTEAHLLPLSLSTASNSPAPPVINSVVIEELDDLNFEWTCPNTRLLFPDPAFPRNFREPIRSAVDKSCLRPIAHREPGMGRPPLRAQDFMANNPEHLELLRRMGVSLIHLKDGRVQLVQHATQAVSAVAAEDGMRFMQEMMELSSQQQKEQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKSSSGVRGFVTVAEPYNGCSELSNGEIVAGRIALLQRGQCMFAEKARHVQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGRNTDDVTLPLLFLFHKEANILLEALKEYKEVEVLLSDKARDREAIFKGKALPGALLDGNGAEEEDCSTEERDHSSPAPSEPVDQSQVTTQEQTAGDSESVQPAEGLGSYVSVDAGEEPPADEDSNNQSVDSLMADWQEDLEAFKQMEKDEL

Target ORF information:

RefSeq Version	XM_009303906.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303906.2

ORF Insert Sequence:

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTTTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCA 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AATGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT TCTTGAATTT 
GCCGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCCATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GAACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTCCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCACA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTAAGTCTC 
ATCCATCTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCGCAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGCCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGAAGCCATA 
TTTAAGGGAA AAGCCCTCCC GGGCGCCCTG TTGGATGGCA ATGGAGCAGA AGAAGAAGAC 
TGTTCCACTG AGGAGAGAGA CCACAGTTCA CCCGCCCCCT CAGAACCCGT TGACCAATCA 
CAAGTGACCA CTCAGGAACA GACAGCAGGC GACTCCGAGT CAGTTCAGCC TGCGGAGGGA 
CTGGGCAGCT ACGTCTCCGT GGATGCAGGG GAGGAGCCTC CAGCAGACGA AGACTCCAAC 
AACCAATCGG TGGACTCGCT GATGGCTGAT TGGCAGGAGG ACTTGGAAGC ATTTAAGCAA 
ATGGAGAAGG ATGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edem3 ( XM_005166932.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166989.2 Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143777
Clone ID Related Accession (Same CDS sequence)	XM_005166932.4
Accession Version	XM_005166932.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166932.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTCTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCG 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AACGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT CCTTGAATTT 
GCTGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCTATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GGACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTTCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCTCA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTGAGTCTC 
ATCCATTTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCACAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGGCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGAAGCCATA 
TTTAAGGGAA AAGCCCTCCC GGGCGCCCTG TTGGATGGCA CTACAGATGG AGCAGAAGAA 
GAAGACTGTT CCACTGAGGA GAGAGACCAC AGTTCACCCG CCCCCTCAGA ACCCGTTGAC 
CAATCACAAG TGACCACTCA GGAACAGACA GCAGGCGACT CCGAGTCAGT TCAGCCTGCG 
GAGGGACTGG GCAGCTACGT CTCCGTGGAT GCAGGGGAGG AGCCTCCAGC AGACGAAGAC 
TCCAACAACC AATCGGTGGA CTCGCTGATG GCTGATTGGC AGGAGGACTT GGAAGCATTT 
AAGCAAATGG AGAAGGATGA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166989.2
CDS	1117..3783
Translation	MWMLHSATMLPAFWLLFFMGTCIRLSETMSRDEKSKLRIQVLEMFDHAYRNYMDHAYPADELMPLTCRGRVRGLEPSRGDVDDALGKFSLTLIDTLDTLALLNKTVEFEEAVRRVVKDVRLDNDIVVSVFETNIRVLGGLLGGHSMAVMLKENGQMVWYQNELLHMAKDLGLRLLPAFNTSSGLPYPRVNLRHGVHGPETRTGTETETCTACAGTIILEFAALSRFTGDPTFEAHARRALDFLWEKRQRNSNLVGTTINIHSGEWVRRDSGVGAGIDSYYEYLMKAYILLGDDQLLLRFNTHYASIMKYISQPPLLLDVHIHKPLLPARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVTKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATRDPYYLEVGRTVLENLNRFARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLFLLFAEEEDLPFNVEDYVFTTEAHLLPLSLSTASNSPAPPVINSVVIEELDDLNFEWTCPNTRLLFPDPAFPRNFREPIRSAVDKSCLRPIAHREPGMGRPPLRAQDFMANNPEHLELLRRMGVSLIHLKDGRVQLVQHATQAVSAVAAEDGMRFMQEMMELSSQQQKEQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKSSSGVRGFVTVAEPYNGCSELSNGEIVAGRIALLQRGQCMFAEKARHVQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGRNTDDVTLPLLFLFHKEGNILLEALKEYKEVEVLLSDKARDREAIFKGKALPGALLDGTTDGAEEEDCSTEERDHSSPAPSEPVDQSQVTTQEQTAGDSESVQPAEGLGSYVSVDAGEEPPADEDSNNQSVDSLMADWQEDLEAFKQMEKDEL

Target ORF information:

RefSeq Version	XM_005166932.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166932.4

ORF Insert Sequence:

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTCTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCG 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AACGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT CCTTGAATTT 
GCTGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCTATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GGACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTTCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCTCA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTGAGTCTC 
ATCCATTTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCACAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGGCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGAAGCCATA 
TTTAAGGGAA AAGCCCTCCC GGGCGCCCTG TTGGATGGCA CTACAGATGG AGCAGAAGAA 
GAAGACTGTT CCACTGAGGA GAGAGACCAC AGTTCACCCG CCCCCTCAGA ACCCGTTGAC 
CAATCACAAG TGACCACTCA GGAACAGACA GCAGGCGACT CCGAGTCAGT TCAGCCTGCG 
GAGGGACTGG GCAGCTACGT CTCCGTGGAT GCAGGGGAGG AGCCTCCAGC AGACGAAGAC 
TCCAACAACC AATCGGTGGA CTCGCTGATG GCTGATTGGC AGGAGGACTT GGAAGCATTT 
AAGCAAATGG AGAAGGATGA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edem3 ( XM_009303906.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302181.2 Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa143778
Clone ID Related Accession (Same CDS sequence)	XM_009303906.3
Accession Version	XM_009303906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTCTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCG 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AACGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT CCTTGAATTT 
GCTGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCTATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GGACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTTCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCTCA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTGAGTCTC 
ATCCATTTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCACAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGGCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGAAGCCATA 
TTTAAGGGAA AAGCCCTCCC GGGCGCCCTG TTGGATGGCA ATGGAGCAGA AGAAGAAGAC 
TGTTCCACTG AGGAGAGAGA CCACAGTTCA CCCGCCCCCT CAGAACCCGT TGACCAATCA 
CAAGTGACCA CTCAGGAACA GACAGCAGGC GACTCCGAGT CAGTTCAGCC TGCGGAGGGA 
CTGGGCAGCT ACGTCTCCGT GGATGCAGGG GAGGAGCCTC CAGCAGACGA AGACTCCAAC 
AACCAATCGG TGGACTCGCT GATGGCTGAT TGGCAGGAGG ACTTGGAAGC ATTTAAGCAA 
ATGGAGAAGG ATGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302181.2
CDS	1117..3777
Translation	MWMLHSATMLPAFWLLFFMGTCIRLSETMSRDEKSKLRIQVLEMFDHAYRNYMDHAYPADELMPLTCRGRVRGLEPSRGDVDDALGKFSLTLIDTLDTLALLNKTVEFEEAVRRVVKDVRLDNDIVVSVFETNIRVLGGLLGGHSMAVMLKENGQMVWYQNELLHMAKDLGLRLLPAFNTSSGLPYPRVNLRHGVHGPETRTGTETETCTACAGTIILEFAALSRFTGDPTFEAHARRALDFLWEKRQRNSNLVGTTINIHSGEWVRRDSGVGAGIDSYYEYLMKAYILLGDDQLLLRFNTHYASIMKYISQPPLLLDVHIHKPLLPARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVTKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATRDPYYLEVGRTVLENLNRFARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLFLLFAEEEDLPFNVEDYVFTTEAHLLPLSLSTASNSPAPPVINSVVIEELDDLNFEWTCPNTRLLFPDPAFPRNFREPIRSAVDKSCLRPIAHREPGMGRPPLRAQDFMANNPEHLELLRRMGVSLIHLKDGRVQLVQHATQAVSAVAAEDGMRFMQEMMELSSQQQKEQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKSSSGVRGFVTVAEPYNGCSELSNGEIVAGRIALLQRGQCMFAEKARHVQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGRNTDDVTLPLLFLFHKEGNILLEALKEYKEVEVLLSDKARDREAIFKGKALPGALLDGNGAEEEDCSTEERDHSSPAPSEPVDQSQVTTQEQTAGDSESVQPAEGLGSYVSVDAGEEPPADEDSNNQSVDSLMADWQEDLEAFKQMEKDEL

Target ORF information:

RefSeq Version	XM_009303906.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303906.3

ORF Insert Sequence:

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTCTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCG 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AACGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT CCTTGAATTT 
GCTGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCTATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GGACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTTCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCTCA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTGAGTCTC 
ATCCATTTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCACAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGGCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGAAGCCATA 
TTTAAGGGAA AAGCCCTCCC GGGCGCCCTG TTGGATGGCA ATGGAGCAGA AGAAGAAGAC 
TGTTCCACTG AGGAGAGAGA CCACAGTTCA CCCGCCCCCT CAGAACCCGT TGACCAATCA 
CAAGTGACCA CTCAGGAACA GACAGCAGGC GACTCCGAGT CAGTTCAGCC TGCGGAGGGA 
CTGGGCAGCT ACGTCTCCGT GGATGCAGGG GAGGAGCCTC CAGCAGACGA AGACTCCAAC 
AACCAATCGG TGGACTCGCT GATGGCTGAT TGGCAGGAGG ACTTGGAAGC ATTTAAGCAA 
ATGGAGAAGG ATGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edem3 ( XM_005166934.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166991.2 Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa143779
Clone ID Related Accession (Same CDS sequence)	XM_005166934.4
Accession Version	XM_005166934.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166934.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTCTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCG 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AACGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT CCTTGAATTT 
GCTGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCTATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GGACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTTCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCTCA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTGAGTCTC 
ATCCATTTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCACAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGGCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGCTACAGAT 
GGAGCAGAAG AAGAAGACTG TTCCACTGAG GAGAGAGACC ACAGTTCACC CGCCCCCTCA 
GAACCCGTTG ACCAATCACA AGTGACCACT CAGGAACAGA CAGCAGGCGA CTCCGAGTCA 
GTTCAGCCTG CGGAGGGACT GGGCAGCTAC GTCTCCGTGG ATGCAGGGGA GGAGCCTCCA 
GCAGACGAAG ACTCCAACAA CCAATCGGTG GACTCGCTGA TGGCTGATTG GCAGGAGGAC 
TTGGAAGCAT TTAAGCAAAT GGAGAAGGAT GAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166991.2
CDS	1117..3735
Translation	MWMLHSATMLPAFWLLFFMGTCIRLSETMSRDEKSKLRIQVLEMFDHAYRNYMDHAYPADELMPLTCRGRVRGLEPSRGDVDDALGKFSLTLIDTLDTLALLNKTVEFEEAVRRVVKDVRLDNDIVVSVFETNIRVLGGLLGGHSMAVMLKENGQMVWYQNELLHMAKDLGLRLLPAFNTSSGLPYPRVNLRHGVHGPETRTGTETETCTACAGTIILEFAALSRFTGDPTFEAHARRALDFLWEKRQRNSNLVGTTINIHSGEWVRRDSGVGAGIDSYYEYLMKAYILLGDDQLLLRFNTHYASIMKYISQPPLLLDVHIHKPLLPARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVTKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATRDPYYLEVGRTVLENLNRFARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLFLLFAEEEDLPFNVEDYVFTTEAHLLPLSLSTASNSPAPPVINSVVIEELDDLNFEWTCPNTRLLFPDPAFPRNFREPIRSAVDKSCLRPIAHREPGMGRPPLRAQDFMANNPEHLELLRRMGVSLIHLKDGRVQLVQHATQAVSAVAAEDGMRFMQEMMELSSQQQKEQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKSSSGVRGFVTVAEPYNGCSELSNGEIVAGRIALLQRGQCMFAEKARHVQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGRNTDDVTLPLLFLFHKEGNILLEALKEYKEVEVLLSDKARDRATDGAEEEDCSTEERDHSSPAPSEPVDQSQVTTQEQTAGDSESVQPAEGLGSYVSVDAGEEPPADEDSNNQSVDSLMADWQEDLEAFKQMEKDEL

Target ORF information:

RefSeq Version	XM_005166934.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166934.4

ORF Insert Sequence:

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTCTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCG 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AACGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT CCTTGAATTT 
GCTGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCTATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GGACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTTCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCTCA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTGAGTCTC 
ATCCATTTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCACAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGGCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGCTACAGAT 
GGAGCAGAAG AAGAAGACTG TTCCACTGAG GAGAGAGACC ACAGTTCACC CGCCCCCTCA 
GAACCCGTTG ACCAATCACA AGTGACCACT CAGGAACAGA CAGCAGGCGA CTCCGAGTCA 
GTTCAGCCTG CGGAGGGACT GGGCAGCTAC GTCTCCGTGG ATGCAGGGGA GGAGCCTCCA 
GCAGACGAAG ACTCCAACAA CCAATCGGTG GACTCGCTGA TGGCTGATTG GCAGGAGGAC 
TTGGAAGCAT TTAAGCAAAT GGAGAAGGAT GAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edem3 ( XM_683183.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_688275.6 Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa143780
Clone ID Related Accession (Same CDS sequence)	XM_683183.8
Accession Version	XM_683183.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_683183.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTCTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCG 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AACGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT CCTTGAATTT 
GCTGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCTATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GGACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTTCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCTCA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTGAGTCTC 
ATCCATTTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCACAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGGCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGATGGAGCA 
GAAGAAGAAG ACTGTTCCAC TGAGGAGAGA GACCACAGTT CACCCGCCCC CTCAGAACCC 
GTTGACCAAT CACAAGTGAC CACTCAGGAA CAGACAGCAG GCGACTCCGA GTCAGTTCAG 
CCTGCGGAGG GACTGGGCAG CTACGTCTCC GTGGATGCAG GGGAGGAGCC TCCAGCAGAC 
GAAGACTCCA ACAACCAATC GGTGGACTCG CTGATGGCTG ATTGGCAGGA GGACTTGGAA 
GCATTTAAGC AAATGGAGAA GGATGAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688275.6
CDS	1117..3729
Translation	MWMLHSATMLPAFWLLFFMGTCIRLSETMSRDEKSKLRIQVLEMFDHAYRNYMDHAYPADELMPLTCRGRVRGLEPSRGDVDDALGKFSLTLIDTLDTLALLNKTVEFEEAVRRVVKDVRLDNDIVVSVFETNIRVLGGLLGGHSMAVMLKENGQMVWYQNELLHMAKDLGLRLLPAFNTSSGLPYPRVNLRHGVHGPETRTGTETETCTACAGTIILEFAALSRFTGDPTFEAHARRALDFLWEKRQRNSNLVGTTINIHSGEWVRRDSGVGAGIDSYYEYLMKAYILLGDDQLLLRFNTHYASIMKYISQPPLLLDVHIHKPLLPARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVTKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATRDPYYLEVGRTVLENLNRFARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLFLLFAEEEDLPFNVEDYVFTTEAHLLPLSLSTASNSPAPPVINSVVIEELDDLNFEWTCPNTRLLFPDPAFPRNFREPIRSAVDKSCLRPIAHREPGMGRPPLRAQDFMANNPEHLELLRRMGVSLIHLKDGRVQLVQHATQAVSAVAAEDGMRFMQEMMELSSQQQKEQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKSSSGVRGFVTVAEPYNGCSELSNGEIVAGRIALLQRGQCMFAEKARHVQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGRNTDDVTLPLLFLFHKEGNILLEALKEYKEVEVLLSDKARDRDGAEEEDCSTEERDHSSPAPSEPVDQSQVTTQEQTAGDSESVQPAEGLGSYVSVDAGEEPPADEDSNNQSVDSLMADWQEDLEAFKQMEKDEL

Target ORF information:

RefSeq Version	XM_683183.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683183.8

ORF Insert Sequence:

ATGTGGATGC TTCATTCTGC AACGATGTTG CCTGCTTTTT GGCTGCTGTT TTTTATGGGA 
ACCTGTATCA GGTTGTCGGA GACCATGAGC AGAGACGAGA AGAGCAAACT GAGGATCCAA 
GTGCTGGAAA TGTTTGACCA CGCTTATCGG AATTACATGG ACCATGCTTA CCCTGCAGAC 
GAACTGATGC CTTTGACTTG CCGTGGGCGA GTACGTGGAC TAGAGCCCAG TCGAGGGGAC 
GTAGATGATG CCCTGGGGAA GTTCTCCCTC ACGCTCATCG ATACTCTTGA CACCCTTGCG 
CTGTTAAATA AGACGGTGGA ATTTGAAGAG GCGGTGAGGC GAGTGGTGAA GGATGTACGC 
TTGGATAATG ACATCGTGGT GTCTGTATTT GAAACCAACA TCCGGGTTCT GGGTGGACTG 
TTGGGAGGCC ATTCGATGGC TGTCATGTTG AAAGAAAATG GACAAATGGT CTGGTACCAG 
AACGAGCTGC TTCACATGGC CAAAGATCTG GGACTCAGAC TGCTGCCTGC ATTTAACACC 
AGCAGCGGCC TGCCTTACCC TAGAGTGAAT CTGCGTCATG GAGTCCATGG ACCTGAGACC 
CGCACAGGCA CAGAGACCGA AACTTGCACC GCCTGTGCAG GGACCATCAT CCTTGAATTT 
GCTGCTTTGA GTCGCTTTAC TGGAGATCCC ACATTTGAGG CTCATGCAAG AAGGGCTCTT 
GATTTCCTTT GGGAAAAGAG ACAGAGGAAC AGTAATCTAG TGGGAACAAC CATCAACATC 
CACTCTGGAG AATGGGTCCG CAGGGACAGT GGAGTTGGGG CAGGCATCGA TTCTTATTAT 
GAGTACTTGA TGAAGGCATA TATATTATTG GGCGATGACC AACTTTTACT ACGCTTTAAC 
ACTCACTATG CATCTATTAT GAAGTATATC AGCCAACCTC CTCTACTACT GGATGTCCAC 
ATCCACAAAC CCCTGCTTCC AGCACGCACA TGGATGGACT CCTTGCTTGC CTTTTTCCCT 
GGACTGCAGG TGTTAAAGGG AGATATCCGG CCAGCCATAG AGACTCATGA AATGCTTTAT 
CAGGTTACCA AGAAACACAA CTTTCTCCCT GAGGCATTCA CCACTGATTT CAGGGTTCAC 
TGGGCTCAGC ACCCCCTGCG GCCAGAGTTT GCAGAAAGCA CTTACTTCCT TTACAAGGCA 
ACCAGAGACC CATATTACCT GGAAGTGGGC CGTACTGTAC TGGAGAACCT GAATCGATTC 
GCTCGAGTTC CCTGTGGCTT TGCTGCTATG AAGGATGTGA GGACGGGGAG CCACGAGGAT 
CGCATGGACT CGTTTTTCCT GGCGGAGATG TTTAAGTACC TGTTTCTGCT GTTTGCTGAA 
GAGGAGGATT TGCCTTTCAA CGTGGAGGAC TACGTCTTTA CCACTGAAGC TCATCTCCTG 
CCTCTGTCCC TCTCCACTGC TTCAAACTCA CCGGCGCCCC CTGTCATTAA CAGCGTGGTT 
ATAGAAGAGC TCGATGACTT AAATTTTGAG TGGACTTGCC CTAACACCCG CCTACTCTTT 
CCTGATCCCG CCTTCCCACG CAATTTTCGT GAGCCAATCA GAAGCGCTGT GGATAAGAGT 
TGCCTGCGCC CCATCGCTCA TCGTGAACCT GGCATGGGCC GTCCTCCTCT CAGGGCTCAG 
GACTTCATGG CCAATAACCC TGAACATCTG GAGCTGCTGA GGCGGATGGG TGTGAGTCTC 
ATCCATTTGA AAGATGGCAG AGTACAGCTT GTTCAGCACG CCACACAGGC GGTGAGTGCA 
GTTGCTGCAG AGGACGGCAT GCGCTTTATG CAGGAAATGA TGGAGCTTTC TAGCCAGCAG 
CAGAAAGAAC AGCTTCCTCC ACGTGCTGTT CAGATCGTCT CACACCCCTT TTTCGGCAGA 
GTGGTTCTGA CCGCAGGCCC CGCACAGTTT GGAATGGACC TTTCCAAAAG CAGCTCTGGA 
GTGCGTGGGT TTGTGACTGT AGCAGAGCCC TATAATGGCT GTTCTGAGTT GAGTAATGGT 
GAGATTGTGG CAGGTCGTAT CGCTCTGCTG CAGAGAGGCC AGTGCATGTT TGCAGAAAAG 
GCAAGGCATG TGCAGAAAGC TGGAGCCATC GGAGGAATTG TCATTGATGA CAATGAAGGC 
AGCAGCAGTG ATACAGCCCC ATTGTTCCAG ATGGCGGGAG ATGGACGGAA CACTGATGAT 
GTCACTTTAC CTCTCCTCTT CCTGTTTCAC AAAGAGGGCA ACATCCTGCT TGAGGCTCTG 
AAGGAATACA AGGAAGTGGA GGTGCTGCTC AGTGATAAAG CTCGAGACCG AGATGGAGCA 
GAAGAAGAAG ACTGTTCCAC TGAGGAGAGA GACCACAGTT CACCCGCCCC CTCAGAACCC 
GTTGACCAAT CACAAGTGAC CACTCAGGAA CAGACAGCAG GCGACTCCGA GTCAGTTCAG 
CCTGCGGAGG GACTGGGCAG CTACGTCTCC GTGGATGCAG GGGAGGAGCC TCCAGCAGAC 
GAAGACTCCA ACAACCAATC GGTGGACTCG CTGATGGCTG ATTGGCAGGA GGACTTGGAA 
GCATTTAAGC AAATGGAGAA GGATGAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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edem3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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