abca1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	abca1a
Entrez Gene ID	558924
Full Name	ATP-binding cassette, sub-family A (ABC1), member 1A
Synonyms	abca1,cb939,im:6795541,wu:fa03d03,wu:fj13h01
General protein information	Preferred Names ATP-binding cassette, sub-family A (ABC1), member 1A Names ATP-binding cassette sub-family A member 1 fa03d03
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001309465.1	NP_001296394.1	ATP-binding cassette sub-family A member 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABCA1	NP_005493.2
Pan troglodytes (chimpanzee)	ABCA1	XP_001138040.1
Rattus norvegicus (Norway rat)	Abca1	NP_835196.1
Gallus gallus (chicken)	ABCA1	NP_989476.1
Xenopus tropicalis (tropical clawed frog)	abca1	XP_002934028.2
Canis lupus familiaris (dog)	ABCA1	XP_005626946.1
Bos taurus (cattle)	ABCA1	NP_001019864.1
Mus musculus (house mouse)	Abca1	NP_038482.3
Arabidopsis thaliana (thale cress)	ABCA1	NP_850354.2
Danio rerio (zebrafish)	abca1b	NP_001108058.2
Danio rerio (zebrafish)	abca1a	XP_005160198.1
Caenorhabditis elegans (roundworm)	abt-2	NP_490949.3

Gene Ontology
Bibliography

Related articles in PubMed

mRNA processing in mutant zebrafish lines generated by chemical and CRISPR-mediated mutagenesis produces unexpected transcripts that escape nonsense-mediated decay.
Anderson JL, Mulligan TS, Shen MC, Wang H, Scahill CM, Tan FJ, Du SJ, Busch-Nentwich EM, Farber SA
PLoS genetics13(11)e1007105(2017 Nov)

Cholesterol lowering by Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in adult zebrafish is associated with improved memory and involves an interplay between npc1l1 and abca1.
Lim FT, Lim SM, Ramasamy K
Food & function8(8)2817-2828(2017 Aug)

Expression and regulation of drug transporters in vertebrate neutrophils.
Foulkes MJ, Henry KM, Rougeot J, Hooper-Greenhill E, Loynes CA, Jeffrey P, Fleming A, Savage CO, Meijer AH, Jones S, Renshaw SA
Scientific reports7(1)4967(2017 Jul)

Role of Intestinal LXR? in Regulating Post-prandial Lipid Excursion and Diet-Induced Hypercholesterolemia and Hepatic Lipid Accumulation.
Ben?tez-Santana T, Hugo SE, Schlegel A
Frontiers in physiology8280(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following abca1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the abca1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa06220	NM_001309465.1 Latest version!	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 1A (abca1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

abca1a ( NM_001309465.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296394.1 Danio rerio ATP-binding cassette, sub-family A (ABC1), member 1A (abca1a), mRNA.

Price & Availability↑

CloneID	ODa06220
Clone ID Related Accession (Same CDS sequence)	NM_001309465.1
Accession Version	NM_001309465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Danio rerio(zebrafish)
Product	ATP-binding cassette sub-family A member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX004781.7. On Jun 5, 2015 this sequence version replaced XM_005160141.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQH01007727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGTCT CCACTCAGTT GGGTTTACTG TTGTGGAAGA ACTTCACCTA CAGACGGAGA 
CAGACTATTC AGCTGCTGGT CGAGATCATA TGGCCACTTT TCATCTTTTT CATCTTGATA 
TCTGTCAGAC TCTACTATCC GCCATACGAG CAACATGAAT GCCATTTTCC CAATAAGGCT 
ATGCCCTCTG CAGGGACTTT ACCGTGGGTT CAGGGCATCA TCTGCAATGC CAACAACCCC 
TGCTTTCGCT ACCCGACTCC AGGAGAGACG CCCGGGGTTG TGGGCAACTT CAATGACTCC 
ATCATCTCCC GACTGTTCAC CGATGCCAAG AAAATTCTCC TCTACAGCCA ACAAGACAAG 
AGCTTCGAAG GTTTCAAAGG ACTCGTTCGA GCCTTGAGAA AACTGCAGAG AAACACTGAA 
GGTTTCAAAC TGAAGGATTT CCTCCATGAT AATGAAACGC TGTCTGCCTT TCTGGAGAAA 
AATGCCACCC TGCCCAAGCA TGCAGTGAGA CAGATAGTGG AGGCCAACGT CAACTTGGAG 
AAGGTGCTTA TTAAAGGATT CGGCGTTCAT CTTACAGACC TGTGCAATTC CACGTCTCTG 
GAAGAGTTTG TGACTATAGC CGATAAAGAC GTGTCTCAAC TGACGCAAAA CATCATCTGC 
CAGTCGCCCG CTGATTGGCT GAACAACGCA GAGCAACACT TCCTGTCCAA CCTGGACTTC 
CTGAAGCCCA TTCGGAAAGA TGTCAAGTCA GACCCAAAGA TCATCAAAGA TGTGTCCATT 
GCTACAGACA GTCTGCTGGA GAGTCTGGGG GCTTTGGCAG TGGAGTTGGC GAGTATGAAG 
AGCTGGCGCG ACATGCGGAA TGAGATCCTC TACCTGACGG GCAACAGCAC GCAGTCACCA 
AACCACATGT ACCAGGCCGT GTCCCGCATC GTCTGCGGCC ATCCGGAGGG TGGCGGATTA 
AAGATCAAGT CCCTCAACTG GTTCGAAGAC AATAACTACA AAGCCCTGTT CGGAAACAAT 
GGCAACGACA GTGACAGCGA GCCCGCATCT GTTTACGACA ACTCCTCCAC GCCGTACTGC 
AACAACCTGA TGAAGGGTCT TGAGTCGAGT CCCATTTCCC GCATGATCTG GAGGGCCCTG 
AAACCGCTGC TCATGGGAAA GATCTTGTAC ACACCAGACA CTCCGGCCAC ACAGAGGATC 
ATACATGAGG TCAATAAGAC GTTTCAGGAG CTCGGTGTAC TCCGTGACCT CGGCGGCATG 
TGGGAAGAAA TGAGACCCAA AATCTGGAAC TTCATGGAGA ACAGTGAGGA GATGGACCTC 
GTCCGGACGC TGCTCCAGAA TAACGCCAGC GCTCGTTTCT TCAATGCTAA GCTGAGCGGG 
ACAGAATGGC GCGTGGAGGA TGTGTCCCGC TTCTTGTCCA AGTCTTCAGA GGACACTCGT 
CCACATGGGA CAGCGTACAC ATGGAGAGAA GTGTTCAACG AGACTGACCA GGCCATACAG 
ACCATCTCCC GCTTCATGGA GTGTGTGAAC TTGGATAAGT TGGAGCCGGT GGCGAACGAG 
GAGCGTCTGG TCAATAAATC CATGCGTCTG CTGGATGACC GCAAGTTTTG GGCAGGAATC 
GTGTTTCCCG ACATGCAATC CAACACGTCT GATCTGCCAC TCAACGTTAA CTATAAGATC 
CGCATGGACA TCGATAACGT GGAGCGAACC AATAAGATCA AGGACGGATA TTGGGATCCG 
GGTCCTCGAG CTGACCCATT TGAGGACTTG CGTTACATCT GGGGCGGCTT CACATACCTG 
CAGGACGTGA TTGAGCACGG GATCATCAGA GCTCTGACCG GCAGCAAGGA AAAAACCGGA 
GTCTACATCC AGCAGATGCC GTATCCCTGC TATGTGGATG ACATTTTCCT GCGGGTCATG 
AGCCGCTCCA TGCCTCTCTT CATGACTCTG GCCTGGATGT ACTCGGTGGC CATCATCATC 
AAGGGTGTGG TGTATGAAAA AGAGGCCCGA CTCAAGGAGA CCATGCGGAT CATGGGTCTG 
GATAACGGCA TCTTGTGGCT GAGCTGGTTC ATCAGCAGCC TGATACCACT GCTCATCAGC 
GCCGCCCTGC TGGTGCTCAT CCTGAAGATG GGGAATCTCC TGCCGTACAG CGACCCCGGC 
GTCATCTACT TGTTCTTGTC CTCTTTTGCG GTGGTGACCA TCATGCAGTG TTTCCTGATC 
AGCACACTCT TCTCTCGTGC CAATCTTGCC GCCGCCTGCG GAGGCATCAT TTACTTCACG 
CTTTACCTGC CCTATGTGCT CTGCGTCGCC TGGCAGAACT ACGTGGGCTT CGGTGCCAAA 
ATAGTAGTGA GTCTGCTGTC TCCAGTGGCG TTCGGCTTCG GCTGTGAGTA TTTCGCTCTG 
TTTGAGGAGC AGGGTGTGGG CATCCAGTGG AGTAATCTGC TGTCCAGCCC CATGCAGGAG 
GACAGCTACA ACCTGACCAC CTCCATCTGC CTCATGCTCT TCGATTCGGT GCTCTACGCC 
GTCATGACCT GGTACATCGA GGCCGTCTTT CCAGGTCAGT ATGGCATCCC TCGACCCTGG 
TACTTCCCTT TCACCAAGTC ATACTGGTGC GGAGAGAAAT GCGGATGGAC AACATCAGTG 
CCAAGTAACA GGAAAGAAAA CGCGGAAGCT GTGTGCATTG AGGAAGAGCC AGCCCATATA 
GATCCTGGTG TTTACATCGA GAACCTGATG AAGATCTACA GCAATGGTAA AGTGGCAGTG 
GACGGTTTGA CTCTGGGCTT TTATGAGGGC CAGATCACTT CTTTCCTTGG CCACAACGGC 
GCTGGAAAAA CCACAACCAT GTCAATCCTC ACCGGCCTGT TTCCACCGAC TTCTGGCACT 
GCGTACATCC AGGGCAAAGA CATCCGCACA GACCTGAATG CCATCCGCCA AAACCTGGGC 
GTCTGTCCTC AGCATAACGT CCTGTTCAGC ATGCTGACCG TGGAGGAGCA CATCTGGTTT 
TATGCGCGTC TGAAGGGTTT GTCTGCGGAG AAGGTGAAGT CTGAGATGGA GCAGATCGTG 
ATGGACCTCG GCCTTCCTCA TAAACGCACA TCTCGCTCCA ACCAGCTCTC AGGAGGAATG 
CAGAGGAAGT TATCAGTCGC GCTGGCCTTC GTCGGAGGCT CAAAAGTGGT GATTCTGGAT 
GAACCGACTG CTGGAGTTGA TCCTTACGCC CGCCGTGGCA TCTGGGACCT GCTGCTGAAA 
TACCGCCAAG GTCGCACCAT CATCCTGTCC ACACACCACA TGGATGAAGC CGATATACTG 
GGCGACCGCA TCGCCATCAT CTCTCATGGC AAGCTGTGCT GCGTGGGCTC CTCTCTGTTC 
CTCAAGACTC AGCTGGGCAC GGGTTACTAC CTGACGCTGG TCAAGAAAGA CTTCGATCTG 
TCCGCCAGCT CCTGCCGCAC CTCCAGCAGC ACGGTCTCCT ACTCCAAGGG AAGCCTCAAG 
AAGGATGACA GCGTATCTGA GAGCAGTTCT GATGCCGGAC TGGGCAGCGA CCATGAAAGC 
GAAACCACCA CCATCGACAT CTCCTTGATC TCCAATGTGA TCTTCAAGCA CGTTCCTACA 
GCCAGACTGG TGGAGGATCT CGGCCACGAA ATCACGTACG TTCTGCCATA TGAATCTGCC 
AAAGATGGAG CATTCGTGGA GCTTTTCCAC GAGATCGACG ACCGGCTCAC AGACCTCAGC 
ATCTCCAGCT ACGGCATCTC GGACACCACT CTCGAAGAGA TTTTCCTGAA AGTGGCTGAG 
GACTGCGGTG TGGATGCTGA AACCTCAGAC GGGACTCTGC CTGCACGCAG GAACCGCAGA 
CATGCATTCG GGGACCATCA GAGCTGCTTG AAGCCTTTTA CTGAAGACGA CTTTGATTTC 
AACGACTCTG AAGGGGATCC AGAGTCTCGT GAGACTGACT GGTTGGATGG TGCTGATGGT 
AAAGGCTCGT ATCAGGTGAA GGGCTGGAGT CTGAAGCGGC AGCAGTTTGT GGCTTTGCTC 
TGGAAACGTT TACTGTACGC CCGACGCTCA CGCAAAGGTT TCTTTGCTCA GATTGTGCTT 
CCCGCTGTGT TCGTGTGCAT CGCTCTGGTC TTCAGCCTCA TCGTTCCTCC TTTCGGGAAA 
TATCCCAGCC TCGCTCTGGA GCCCAGCATG TATGAGGAGC AGTTCACCTT TATCAGTAAT 
GACGCACCAG AAGACAGACA CACAAACAGG CTGCTTGATG CTCTGAAGGA CAGTCCGGCT 
TATACTGACC AGTGCACAGA TGGGAAAAAC ACAGCTAGCA GATCGTGTCC GATTAAAGAT 
GGAGAATGGC TGGTTCCAGA GGTTACCGAG AGCATTGAGG CAATATTCCT GAATGGAAAC 
TGGAGTATGG AAAACCCTTC TCCTCTGTGT GAGTGCAGCT GTGAAGGACG GAAAAAGATG 
CTTCCGGAGT GTCCGCCTGG AGCAGGAGGC CTTCCACCGC CTCAGATTAA AGTCACCCAT 
GATGAAACCC TGCAGAATCT GTCTGGAAGA AACATTTCAG ACTATTTGGT GAAAACATAC 
GCACAGATTA TTGGGAAAAG TTTGAAGAAC AAGCTCTGGG TCAACGAATT CAGATATGGT 
GGCTTCTCAT TGGGTGCACG AAACACACAG GCTCTCCCAC CTGGCGATGA GCTCACTGAT 
GCCATCTCTC ACATCCGCAG CCGCTTCAGT CTTGAGTCGG GCACCGCAGC CGACCGCTTT 
CTGGGAAGCC TGTCAACTTT TATCCGAGGT CTGGATACCA ATAACAATGT GAAGATCTGG 
TTCAATAATA AAGGCTGGCA CAGTATCGGA TCATTCCTCA ACGTCATGAA CAATGCCGTC 
CTGCGTGCTA ATCTTCCTCC CGGCCTGGAT CGCTCCAAAT TTGGCATCAA CGCATTTAAC 
CATCCACTCA ACCTCACCAA AGAGCAGCTC TCGCATGTGG CACTAATGAC CACCTCGGTG 
GATGTGCTGG TGTCCATCTG CGTCATCTTC GCCATGTCCT TCGTTCCAGC CAGTTTCGTG 
GTGTTCCTCA TCCAGGAGAG AGTGAATAAA GCCAAACACA TGCAGTTCAT CAGCGGAGTG 
CAGCCGTTTC TCTACTGGCT GGCCAACTTT GTCTGGGACA TGTGCAACTA CATTGTCCCG 
GCGACTCTGG TGATCATCAT CTTCGTCTGT TTCCAACAAG AGGCATATGT GTCTTCGACA 
AACCTGCCGG TTCTTGCTCT TCTGCTGCTT CTGTATGGAT GGTCCATAAC GCCCCTGATG 
TACCCAGCTT CATTCTTCTT CAAGATCCCA AGTACAGCAT ATGTGGTTCT GACCAGCGTC 
AACATCCTCA TTGGGATAAA CGGCAGCGTA TCCACATTCG TCCTGGAGCT TTTCGGCAGC 
AATGAGATCG GCGGCATCAA TGACATCTTG AAGAACGTGT TTCTGATCTT CCCTCATTTC 
TGTCTGGGTC GAGGACTCAT TGATATGGTG AAGAACCAAG CTATGGCTGA CGCGCTGGAG 
CGATTCGGAG AGAATCGTTT CCGCTCTCCT CTGGCGTGGG ACATGGTGGG CAAGAATCTG 
TTCGCTATGG CCATCGAGGG CGTGATTTTC TTCAGCATCA CCGTCCTCAT CCAGTACCGC 
TTCTGCATTA AGGCCAGGTC ACTTAGCACT AAGCTGAAGC CAATCGGAGA GGAGGATGAG 
GACGTAGCCC GAGAGAGGCA AAGGATTCTG GGAGGAGGAG GACAGACTGA TATCCTGGAG 
CTCAGGCAGC TCACCAAGGT GTATAAGAGG AAGCAGAAGC CAGCTGTAGA CAGACTGTGT 
GTGGGAATCC CACCTGGAGA GTGTTTTGGG TTGCTGGGTG TGAATGGAGC CGGAAAGACG 
AGCACCTTCA AGATGCTAAC CGGAGACTCT GTGGTCACCA GCGGAGAGGC CTACTTAGCA 
GGAAACAGCG TGCTGACGGA GATTGATGAA GTGCATCAGA ATATGGGCTA CTGCCCGCAG 
TTTGATGCCA TCAATGACCT GCTGACGGGC AGAGAGCATC TGGAGTTTTA TGCCATCCTG 
CGTGGCGTCC CTGAGAAAGA AGTGTGCAAG GTCGCAGATT GGGGCATCCG CAAACTGGGC 
CTGATGAAGT ATGTGGACAA AGCAGCAGGA AGCTACAGCG GAGGAAACAT GAGGAAGCTT 
TCCACCGCCA TGGCGCTCAT TGGAGGACCG CCGGTGGTGT TCTTGGACGA ACCCACCACT 
GGGATGGACC CCAAAGCACG GCGGGCCCTG TGGAACTGCA TCCTCAGCAT CATTAAAGAG 
GGACGCTCCG TCGTGCTGAC CTCACACAGT ATGGAGGAAT GTGAAGCTCT TTGCACTCGG 
ATGGCCATCA TGGTGAACGG CAGGTTCCGC TGTCTGGGCA GTGTGCAGCA TTTAAAGAAC 
AAGTTTGGTG ACGGGTACAC CATCATCCTG CGGGTGGCAG GCGCCGATCC GCGGCTGGAG 
CCGGTGATGG AGTTCATCGA GCGGGAGCTG CCGGGCAGTA CGCTGAAGGA GAAACACCGA 
AACATGCTGC AGTACCAGCT GTCCTCATCC CTGACCTCCC TCGCTCGCAT CTTCAACATC 
CTCTCCAAGA ACAAAGAGCA GCTCCACATC GAGGACTACT CCGTCTCACA GACCACTTTA 
GACCAAGTTT TTGTCAATTT TGCCAAGGAC CAAAGTGACG AGGACCACTT AAAAGACATT 
TCCATGAACA AAAATGATGC CGTTGTGGAC ATTTCGAAAC TGAACGCCTT CCTCACAGAC 
GAGAAGGCCA AAGAGACAGT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296394.1
CDS	199..7005
Translation	MAVSTQLGLLLWKNFTYRRRQTIQLLVEIIWPLFIFFILISVRLYYPPYEQHECHFPNKAMPSAGTLPWVQGIICNANNPCFRYPTPGETPGVVGNFNDSIISRLFTDAKKILLYSQQDKSFEGFKGLVRALRKLQRNTEGFKLKDFLHDNETLSAFLEKNATLPKHAVRQIVEANVNLEKVLIKGFGVHLTDLCNSTSLEEFVTIADKDVSQLTQNIICQSPADWLNNAEQHFLSNLDFLKPIRKDVKSDPKIIKDVSIATDSLLESLGALAVELASMKSWRDMRNEILYLTGNSTQSPNHMYQAVSRIVCGHPEGGGLKIKSLNWFEDNNYKALFGNNGNDSDSEPASVYDNSSTPYCNNLMKGLESSPISRMIWRALKPLLMGKILYTPDTPATQRIIHEVNKTFQELGVLRDLGGMWEEMRPKIWNFMENSEEMDLVRTLLQNNASARFFNAKLSGTEWRVEDVSRFLSKSSEDTRPHGTAYTWREVFNETDQAIQTISRFMECVNLDKLEPVANEERLVNKSMRLLDDRKFWAGIVFPDMQSNTSDLPLNVNYKIRMDIDNVERTNKIKDGYWDPGPRADPFEDLRYIWGGFTYLQDVIEHGIIRALTGSKEKTGVYIQQMPYPCYVDDIFLRVMSRSMPLFMTLAWMYSVAIIIKGVVYEKEARLKETMRIMGLDNGILWLSWFISSLIPLLISAALLVLILKMGNLLPYSDPGVIYLFLSSFAVVTIMQCFLISTLFSRANLAAACGGIIYFTLYLPYVLCVAWQNYVGFGAKIVVSLLSPVAFGFGCEYFALFEEQGVGIQWSNLLSSPMQEDSYNLTTSICLMLFDSVLYAVMTWYIEAVFPGQYGIPRPWYFPFTKSYWCGEKCGWTTSVPSNRKENAEAVCIEEEPAHIDPGVYIENLMKIYSNGKVAVDGLTLGFYEGQITSFLGHNGAGKTTTMSILTGLFPPTSGTAYIQGKDIRTDLNAIRQNLGVCPQHNVLFSMLTVEEHIWFYARLKGLSAEKVKSEMEQIVMDLGLPHKRTSRSNQLSGGMQRKLSVALAFVGGSKVVILDEPTAGVDPYARRGIWDLLLKYRQGRTIILSTHHMDEADILGDRIAIISHGKLCCVGSSLFLKTQLGTGYYLTLVKKDFDLSASSCRTSSSTVSYSKGSLKKDDSVSESSSDAGLGSDHESETTTIDISLISNVIFKHVPTARLVEDLGHEITYVLPYESAKDGAFVELFHEIDDRLTDLSISSYGISDTTLEEIFLKVAEDCGVDAETSDGTLPARRNRRHAFGDHQSCLKPFTEDDFDFNDSEGDPESRETDWLDGADGKGSYQVKGWSLKRQQFVALLWKRLLYARRSRKGFFAQIVLPAVFVCIALVFSLIVPPFGKYPSLALEPSMYEEQFTFISNDAPEDRHTNRLLDALKDSPAYTDQCTDGKNTASRSCPIKDGEWLVPEVTESIEAIFLNGNWSMENPSPLCECSCEGRKKMLPECPPGAGGLPPPQIKVTHDETLQNLSGRNISDYLVKTYAQIIGKSLKNKLWVNEFRYGGFSLGARNTQALPPGDELTDAISHIRSRFSLESGTAADRFLGSLSTFIRGLDTNNNVKIWFNNKGWHSIGSFLNVMNNAVLRANLPPGLDRSKFGINAFNHPLNLTKEQLSHVALMTTSVDVLVSICVIFAMSFVPASFVVFLIQERVNKAKHMQFISGVQPFLYWLANFVWDMCNYIVPATLVIIIFVCFQQEAYVSSTNLPVLALLLLLYGWSITPLMYPASFFFKIPSTAYVVLTSVNILIGINGSVSTFVLELFGSNEIGGINDILKNVFLIFPHFCLGRGLIDMVKNQAMADALERFGENRFRSPLAWDMVGKNLFAMAIEGVIFFSITVLIQYRFCIKARSLSTKLKPIGEEDEDVARERQRILGGGGQTDILELRQLTKVYKRKQKPAVDRLCVGIPPGECFGLLGVNGAGKTSTFKMLTGDSVVTSGEAYLAGNSVLTEIDEVHQNMGYCPQFDAINDLLTGREHLEFYAILRGVPEKEVCKVADWGIRKLGLMKYVDKAAGSYSGGNMRKLSTAMALIGGPPVVFLDEPTTGMDPKARRALWNCILSIIKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLKNKFGDGYTIILRVAGADPRLEPVMEFIERELPGSTLKEKHRNMLQYQLSSSLTSLARIFNILSKNKEQLHIEDYSVSQTTLDQVFVNFAKDQSDEDHLKDISMNKNDAVVDISKLNAFLTDEKAKETVV

Target ORF information:

RefSeq Version	NM_001309465.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 1A (abca1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309465.1

ORF Insert Sequence:

ATGGCTGTCT CCACTCAGTT GGGTTTACTG TTGTGGAAGA ACTTCACCTA CAGACGGAGA 
CAGACTATTC AGCTGCTGGT CGAGATCATA TGGCCACTTT TCATCTTTTT CATCTTGATA 
TCTGTCAGAC TCTACTATCC GCCATACGAG CAACATGAAT GCCATTTTCC CAATAAGGCT 
ATGCCCTCTG CAGGGACTTT ACCGTGGGTT CAGGGCATCA TCTGCAATGC CAACAACCCC 
TGCTTTCGCT ACCCGACTCC AGGAGAGACG CCCGGGGTTG TGGGCAACTT CAATGACTCC 
ATCATCTCCC GACTGTTCAC CGATGCCAAG AAAATTCTCC TCTACAGCCA ACAAGACAAG 
AGCTTCGAAG GTTTCAAAGG ACTCGTTCGA GCCTTGAGAA AACTGCAGAG AAACACTGAA 
GGTTTCAAAC TGAAGGATTT CCTCCATGAT AATGAAACGC TGTCTGCCTT TCTGGAGAAA 
AATGCCACCC TGCCCAAGCA TGCAGTGAGA CAGATAGTGG AGGCCAACGT CAACTTGGAG 
AAGGTGCTTA TTAAAGGATT CGGCGTTCAT CTTACAGACC TGTGCAATTC CACGTCTCTG 
GAAGAGTTTG TGACTATAGC CGATAAAGAC GTGTCTCAAC TGACGCAAAA CATCATCTGC 
CAGTCGCCCG CTGATTGGCT GAACAACGCA GAGCAACACT TCCTGTCCAA CCTGGACTTC 
CTGAAGCCCA TTCGGAAAGA TGTCAAGTCA GACCCAAAGA TCATCAAAGA TGTGTCCATT 
GCTACAGACA GTCTGCTGGA GAGTCTGGGG GCTTTGGCAG TGGAGTTGGC GAGTATGAAG 
AGCTGGCGCG ACATGCGGAA TGAGATCCTC TACCTGACGG GCAACAGCAC GCAGTCACCA 
AACCACATGT ACCAGGCCGT GTCCCGCATC GTCTGCGGCC ATCCGGAGGG TGGCGGATTA 
AAGATCAAGT CCCTCAACTG GTTCGAAGAC AATAACTACA AAGCCCTGTT CGGAAACAAT 
GGCAACGACA GTGACAGCGA GCCCGCATCT GTTTACGACA ACTCCTCCAC GCCGTACTGC 
AACAACCTGA TGAAGGGTCT TGAGTCGAGT CCCATTTCCC GCATGATCTG GAGGGCCCTG 
AAACCGCTGC TCATGGGAAA GATCTTGTAC ACACCAGACA CTCCGGCCAC ACAGAGGATC 
ATACATGAGG TCAATAAGAC GTTTCAGGAG CTCGGTGTAC TCCGTGACCT CGGCGGCATG 
TGGGAAGAAA TGAGACCCAA AATCTGGAAC TTCATGGAGA ACAGTGAGGA GATGGACCTC 
GTCCGGACGC TGCTCCAGAA TAACGCCAGC GCTCGTTTCT TCAATGCTAA GCTGAGCGGG 
ACAGAATGGC GCGTGGAGGA TGTGTCCCGC TTCTTGTCCA AGTCTTCAGA GGACACTCGT 
CCACATGGGA CAGCGTACAC ATGGAGAGAA GTGTTCAACG AGACTGACCA GGCCATACAG 
ACCATCTCCC GCTTCATGGA GTGTGTGAAC TTGGATAAGT TGGAGCCGGT GGCGAACGAG 
GAGCGTCTGG TCAATAAATC CATGCGTCTG CTGGATGACC GCAAGTTTTG GGCAGGAATC 
GTGTTTCCCG ACATGCAATC CAACACGTCT GATCTGCCAC TCAACGTTAA CTATAAGATC 
CGCATGGACA TCGATAACGT GGAGCGAACC AATAAGATCA AGGACGGATA TTGGGATCCG 
GGTCCTCGAG CTGACCCATT TGAGGACTTG CGTTACATCT GGGGCGGCTT CACATACCTG 
CAGGACGTGA TTGAGCACGG GATCATCAGA GCTCTGACCG GCAGCAAGGA AAAAACCGGA 
GTCTACATCC AGCAGATGCC GTATCCCTGC TATGTGGATG ACATTTTCCT GCGGGTCATG 
AGCCGCTCCA TGCCTCTCTT CATGACTCTG GCCTGGATGT ACTCGGTGGC CATCATCATC 
AAGGGTGTGG TGTATGAAAA AGAGGCCCGA CTCAAGGAGA CCATGCGGAT CATGGGTCTG 
GATAACGGCA TCTTGTGGCT GAGCTGGTTC ATCAGCAGCC TGATACCACT GCTCATCAGC 
GCCGCCCTGC TGGTGCTCAT CCTGAAGATG GGGAATCTCC TGCCGTACAG CGACCCCGGC 
GTCATCTACT TGTTCTTGTC CTCTTTTGCG GTGGTGACCA TCATGCAGTG TTTCCTGATC 
AGCACACTCT TCTCTCGTGC CAATCTTGCC GCCGCCTGCG GAGGCATCAT TTACTTCACG 
CTTTACCTGC CCTATGTGCT CTGCGTCGCC TGGCAGAACT ACGTGGGCTT CGGTGCCAAA 
ATAGTAGTGA GTCTGCTGTC TCCAGTGGCG TTCGGCTTCG GCTGTGAGTA TTTCGCTCTG 
TTTGAGGAGC AGGGTGTGGG CATCCAGTGG AGTAATCTGC TGTCCAGCCC CATGCAGGAG 
GACAGCTACA ACCTGACCAC CTCCATCTGC CTCATGCTCT TCGATTCGGT GCTCTACGCC 
GTCATGACCT GGTACATCGA GGCCGTCTTT CCAGGTCAGT ATGGCATCCC TCGACCCTGG 
TACTTCCCTT TCACCAAGTC ATACTGGTGC GGAGAGAAAT GCGGATGGAC AACATCAGTG 
CCAAGTAACA GGAAAGAAAA CGCGGAAGCT GTGTGCATTG AGGAAGAGCC AGCCCATATA 
GATCCTGGTG TTTACATCGA GAACCTGATG AAGATCTACA GCAATGGTAA AGTGGCAGTG 
GACGGTTTGA CTCTGGGCTT TTATGAGGGC CAGATCACTT CTTTCCTTGG CCACAACGGC 
GCTGGAAAAA CCACAACCAT GTCAATCCTC ACCGGCCTGT TTCCACCGAC TTCTGGCACT 
GCGTACATCC AGGGCAAAGA CATCCGCACA GACCTGAATG CCATCCGCCA AAACCTGGGC 
GTCTGTCCTC AGCATAACGT CCTGTTCAGC ATGCTGACCG TGGAGGAGCA CATCTGGTTT 
TATGCGCGTC TGAAGGGTTT GTCTGCGGAG AAGGTGAAGT CTGAGATGGA GCAGATCGTG 
ATGGACCTCG GCCTTCCTCA TAAACGCACA TCTCGCTCCA ACCAGCTCTC AGGAGGAATG 
CAGAGGAAGT TATCAGTCGC GCTGGCCTTC GTCGGAGGCT CAAAAGTGGT GATTCTGGAT 
GAACCGACTG CTGGAGTTGA TCCTTACGCC CGCCGTGGCA TCTGGGACCT GCTGCTGAAA 
TACCGCCAAG GTCGCACCAT CATCCTGTCC ACACACCACA TGGATGAAGC CGATATACTG 
GGCGACCGCA TCGCCATCAT CTCTCATGGC AAGCTGTGCT GCGTGGGCTC CTCTCTGTTC 
CTCAAGACTC AGCTGGGCAC GGGTTACTAC CTGACGCTGG TCAAGAAAGA CTTCGATCTG 
TCCGCCAGCT CCTGCCGCAC CTCCAGCAGC ACGGTCTCCT ACTCCAAGGG AAGCCTCAAG 
AAGGATGACA GCGTATCTGA GAGCAGTTCT GATGCCGGAC TGGGCAGCGA CCATGAAAGC 
GAAACCACCA CCATCGACAT CTCCTTGATC TCCAATGTGA TCTTCAAGCA CGTTCCTACA 
GCCAGACTGG TGGAGGATCT CGGCCACGAA ATCACGTACG TTCTGCCATA TGAATCTGCC 
AAAGATGGAG CATTCGTGGA GCTTTTCCAC GAGATCGACG ACCGGCTCAC AGACCTCAGC 
ATCTCCAGCT ACGGCATCTC GGACACCACT CTCGAAGAGA TTTTCCTGAA AGTGGCTGAG 
GACTGCGGTG TGGATGCTGA AACCTCAGAC GGGACTCTGC CTGCACGCAG GAACCGCAGA 
CATGCATTCG GGGACCATCA GAGCTGCTTG AAGCCTTTTA CTGAAGACGA CTTTGATTTC 
AACGACTCTG AAGGGGATCC AGAGTCTCGT GAGACTGACT GGTTGGATGG TGCTGATGGT 
AAAGGCTCGT ATCAGGTGAA GGGCTGGAGT CTGAAGCGGC AGCAGTTTGT GGCTTTGCTC 
TGGAAACGTT TACTGTACGC CCGACGCTCA CGCAAAGGTT TCTTTGCTCA GATTGTGCTT 
CCCGCTGTGT TCGTGTGCAT CGCTCTGGTC TTCAGCCTCA TCGTTCCTCC TTTCGGGAAA 
TATCCCAGCC TCGCTCTGGA GCCCAGCATG TATGAGGAGC AGTTCACCTT TATCAGTAAT 
GACGCACCAG AAGACAGACA CACAAACAGG CTGCTTGATG CTCTGAAGGA CAGTCCGGCT 
TATACTGACC AGTGCACAGA TGGGAAAAAC ACAGCTAGCA GATCGTGTCC GATTAAAGAT 
GGAGAATGGC TGGTTCCAGA GGTTACCGAG AGCATTGAGG CAATATTCCT GAATGGAAAC 
TGGAGTATGG AAAACCCTTC TCCTCTGTGT GAGTGCAGCT GTGAAGGACG GAAAAAGATG 
CTTCCGGAGT GTCCGCCTGG AGCAGGAGGC CTTCCACCGC CTCAGATTAA AGTCACCCAT 
GATGAAACCC TGCAGAATCT GTCTGGAAGA AACATTTCAG ACTATTTGGT GAAAACATAC 
GCACAGATTA TTGGGAAAAG TTTGAAGAAC AAGCTCTGGG TCAACGAATT CAGATATGGT 
GGCTTCTCAT TGGGTGCACG AAACACACAG GCTCTCCCAC CTGGCGATGA GCTCACTGAT 
GCCATCTCTC ACATCCGCAG CCGCTTCAGT CTTGAGTCGG GCACCGCAGC CGACCGCTTT 
CTGGGAAGCC TGTCAACTTT TATCCGAGGT CTGGATACCA ATAACAATGT GAAGATCTGG 
TTCAATAATA AAGGCTGGCA CAGTATCGGA TCATTCCTCA ACGTCATGAA CAATGCCGTC 
CTGCGTGCTA ATCTTCCTCC CGGCCTGGAT CGCTCCAAAT TTGGCATCAA CGCATTTAAC 
CATCCACTCA ACCTCACCAA AGAGCAGCTC TCGCATGTGG CACTAATGAC CACCTCGGTG 
GATGTGCTGG TGTCCATCTG CGTCATCTTC GCCATGTCCT TCGTTCCAGC CAGTTTCGTG 
GTGTTCCTCA TCCAGGAGAG AGTGAATAAA GCCAAACACA TGCAGTTCAT CAGCGGAGTG 
CAGCCGTTTC TCTACTGGCT GGCCAACTTT GTCTGGGACA TGTGCAACTA CATTGTCCCG 
GCGACTCTGG TGATCATCAT CTTCGTCTGT TTCCAACAAG AGGCATATGT GTCTTCGACA 
AACCTGCCGG TTCTTGCTCT TCTGCTGCTT CTGTATGGAT GGTCCATAAC GCCCCTGATG 
TACCCAGCTT CATTCTTCTT CAAGATCCCA AGTACAGCAT ATGTGGTTCT GACCAGCGTC 
AACATCCTCA TTGGGATAAA CGGCAGCGTA TCCACATTCG TCCTGGAGCT TTTCGGCAGC 
AATGAGATCG GCGGCATCAA TGACATCTTG AAGAACGTGT TTCTGATCTT CCCTCATTTC 
TGTCTGGGTC GAGGACTCAT TGATATGGTG AAGAACCAAG CTATGGCTGA CGCGCTGGAG 
CGATTCGGAG AGAATCGTTT CCGCTCTCCT CTGGCGTGGG ACATGGTGGG CAAGAATCTG 
TTCGCTATGG CCATCGAGGG CGTGATTTTC TTCAGCATCA CCGTCCTCAT CCAGTACCGC 
TTCTGCATTA AGGCCAGGTC ACTTAGCACT AAGCTGAAGC CAATCGGAGA GGAGGATGAG 
GACGTAGCCC GAGAGAGGCA AAGGATTCTG GGAGGAGGAG GACAGACTGA TATCCTGGAG 
CTCAGGCAGC TCACCAAGGT GTATAAGAGG AAGCAGAAGC CAGCTGTAGA CAGACTGTGT 
GTGGGAATCC CACCTGGAGA GTGTTTTGGG TTGCTGGGTG TGAATGGAGC CGGAAAGACG 
AGCACCTTCA AGATGCTAAC CGGAGACTCT GTGGTCACCA GCGGAGAGGC CTACTTAGCA 
GGAAACAGCG TGCTGACGGA GATTGATGAA GTGCATCAGA ATATGGGCTA CTGCCCGCAG 
TTTGATGCCA TCAATGACCT GCTGACGGGC AGAGAGCATC TGGAGTTTTA TGCCATCCTG 
CGTGGCGTCC CTGAGAAAGA AGTGTGCAAG GTCGCAGATT GGGGCATCCG CAAACTGGGC 
CTGATGAAGT ATGTGGACAA AGCAGCAGGA AGCTACAGCG GAGGAAACAT GAGGAAGCTT 
TCCACCGCCA TGGCGCTCAT TGGAGGACCG CCGGTGGTGT TCTTGGACGA ACCCACCACT 
GGGATGGACC CCAAAGCACG GCGGGCCCTG TGGAACTGCA TCCTCAGCAT CATTAAAGAG 
GGACGCTCCG TCGTGCTGAC CTCACACAGT ATGGAGGAAT GTGAAGCTCT TTGCACTCGG 
ATGGCCATCA TGGTGAACGG CAGGTTCCGC TGTCTGGGCA GTGTGCAGCA TTTAAAGAAC 
AAGTTTGGTG ACGGGTACAC CATCATCCTG CGGGTGGCAG GCGCCGATCC GCGGCTGGAG 
CCGGTGATGG AGTTCATCGA GCGGGAGCTG CCGGGCAGTA CGCTGAAGGA GAAACACCGA 
AACATGCTGC AGTACCAGCT GTCCTCATCC CTGACCTCCC TCGCTCGCAT CTTCAACATC 
CTCTCCAAGA ACAAAGAGCA GCTCCACATC GAGGACTACT CCGTCTCACA GACCACTTTA 
GACCAAGTTT TTGTCAATTT TGCCAAGGAC CAAAGTGACG AGGACCACTT AAAAGACATT 
TCCATGAACA AAAATGATGC CGTTGTGGAC ATTTCGAAAC TGAACGCCTT CCTCACAGAC 
GAGAAGGCCA AAGAGACAGT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

mRNA processing in mutant zebrafish lines generated by chemical and CRISPR-mediated mutagenesis produces unexpected transcripts that escape nonsense-mediated decay.
PLoS genetics13(11)e1007105(2017 Nov)
Anderson JL,Mulligan TS,Shen MC,Wang H,Scahill CM,Tan FJ,Du SJ,Busch-Nentwich EM,Farber SA

Cholesterol lowering by Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in adult zebrafish is associated with improved memory and involves an interplay between npc1l1 and abca1.
Food & function8(8)2817-2828(2017 Aug)
Lim FT,Lim SM,Ramasamy K

Expression and regulation of drug transporters in vertebrate neutrophils.
Scientific reports7(1)4967(2017 Jul)
Foulkes MJ,Henry KM,Rougeot J,Hooper-Greenhill E,Loynes CA,Jeffrey P,Fleming A,Savage CO,Meijer AH,Jones S,Renshaw SA

Role of Intestinal LXR? in Regulating Post-prandial Lipid Excursion and Diet-Induced Hypercholesterolemia and Hepatic Lipid Accumulation.
Frontiers in physiology8280(2017)
Ben?tez-Santana T,Hugo SE,Schlegel A