spire1a cDNA ORF clone, Danio rerio(zebrafish)

The following spire1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spire1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa03142 NM_001044847.2
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa24280 XM_005159621.4
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa24281 XM_005159622.4
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa24283 XM_005159624.4
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42704 XM_009294320.3
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42705 XM_009294321.3
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42706 XM_009294322.3
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42707 XM_009294323.3
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42708 XM_009294324.3
Latest version!
Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
ODa42704 XM_009294320.2 Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42705 XM_009294321.2 Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa24280 XM_005159621.3 Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42706 XM_009294322.2 Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa24283 XM_005159624.3 Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42707 XM_009294323.2 Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa24281 XM_005159622.3 Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa42708 XM_009294324.2 Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa03142
    Clone ID Related Accession (Same CDS sequence) NM_001044847.2
    Accession Version NM_001044847.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085426.1 and BC124674.1. On Feb 13, 2008 this sequence version replaced NM_001044847.1. ##Evidence-Data-START## Transcript exon combination :: BC085426.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTATAGTCA ACCGATCAAC GAGGAGCAAG CCTGGGCCGT GTGTTACCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TTTGGATTAT GGCCTGAAGG AGCACGAGGA GAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACCGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTCTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGTCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTT CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCAAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGCGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATGAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCTGATGG ACCTCGCAAG TTGGCCATCA GTACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAGG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACACACAC
    ACACTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001038312.2
    CDS140..2308
    Translation

    Target ORF information:

    RefSeq Version NM_001044847.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001044847.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTATAGTCA ACCGATCAAC GAGGAGCAAG CCTGGGCCGT GTGTTACCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TTTGGATTAT GGCCTGAAGG AGCACGAGGA GAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACCGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTCTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGTCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTT CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCAAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGCGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATGAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCTGATGG ACCTCGCAAG TTGGCCATCA GTACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAGG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACACACAC
    ACACTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa24280
    Clone ID Related Accession (Same CDS sequence) XM_005159621.4 , XM_005159621.3
    Accession Version XM_005159621.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159621.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATAAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCAGATGG ACCTCGCAAG TTGGCCATCA GCACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAAG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGATGTCT
    TCAGACGGCA AGGCTCACGC ACTAGGCAGC GGTCATGCGG AGGAGTTCTG CTTCCCTGTC
    GAGTGTTTGA CTCTGACCGT GGAGGAGGTG ATGCACATCA GACAGGTTCT TGTCAAAGCC
    GAACTGGAGA AGTTCCAGCA GTATAAAGAC ATTTATAATG CACTGAAGAA AGGAAAGCTT
    TGCTTCTCAT GTCGGTCGAA GAAGTTCTCA TTATTTACCT GGTCCTACAC CTGTCAGTTC
    TGTAAGAGGC CGGTTTGCTC ACAGTGCTGC AAGAAGATGA AATTGCCCTC CAAGCCTCAC
    GCCAGCCTGC CCATCTCCTC TTTGGGTCCC AGTATCCTGC CCAAAAAAGA GCCCGGAGCA
    TCATCAGCCC CGACAGATAA AACCTCCTCC ACATCCAGCC ACAAGAAAAA CAGCCTTCAG
    CGCTCATTGT CTAGATCATC AAAACACGGC GATCGCTCGT CTTCTAAAGA CGAGCTGGAG
    CTTCCGGAGC AGTTCACCGA GGACTGGAGC ACCATGGAGG TCTGCGTGGA CTGCAAAAAA
    TTCATCAACG ACATCATCAG CAACAGCCGC CGCAACCTGT CCACCAAACG TGCCCGCCTG
    CACCGCCGGA CACACTCCGT CTACTCGTCC AGCACCAGCT CCTCCAACTA CAAACCAACC
    GAGCGCACCA TCAAAGAGGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159678.1
    CDS182..2485
    Translation

    Target ORF information:

    RefSeq Version XM_005159621.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159621.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATAAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCAGATGG ACCTCGCAAG TTGGCCATCA GCACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAAG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGATGTCT
    TCAGACGGCA AGGCTCACGC ACTAGGCAGC GGTCATGCGG AGGAGTTCTG CTTCCCTGTC
    GAGTGTTTGA CTCTGACCGT GGAGGAGGTG ATGCACATCA GACAGGTTCT TGTCAAAGCC
    GAACTGGAGA AGTTCCAGCA GTATAAAGAC ATTTATAATG CACTGAAGAA AGGAAAGCTT
    TGCTTCTCAT GTCGGTCGAA GAAGTTCTCA TTATTTACCT GGTCCTACAC CTGTCAGTTC
    TGTAAGAGGC CGGTTTGCTC ACAGTGCTGC AAGAAGATGA AATTGCCCTC CAAGCCTCAC
    GCCAGCCTGC CCATCTCCTC TTTGGGTCCC AGTATCCTGC CCAAAAAAGA GCCCGGAGCA
    TCATCAGCCC CGACAGATAA AACCTCCTCC ACATCCAGCC ACAAGAAAAA CAGCCTTCAG
    CGCTCATTGT CTAGATCATC AAAACACGGC GATCGCTCGT CTTCTAAAGA CGAGCTGGAG
    CTTCCGGAGC AGTTCACCGA GGACTGGAGC ACCATGGAGG TCTGCGTGGA CTGCAAAAAA
    TTCATCAACG ACATCATCAG CAACAGCCGC CGCAACCTGT CCACCAAACG TGCCCGCCTG
    CACCGCCGGA CACACTCCGT CTACTCGTCC AGCACCAGCT CCTCCAACTA CAAACCAACC
    GAGCGCACCA TCAAAGAGGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa24280
    Clone ID Related Accession (Same CDS sequence) XM_005159621.4 , XM_005159621.3
    Accession Version XM_005159621.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein spire homolog 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159621.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATAAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCAGATGG ACCTCGCAAG TTGGCCATCA GCACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAAG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGATGTCT
    TCAGACGGCA AGGCTCACGC ACTAGGCAGC GGTCATGCGG AGGAGTTCTG CTTCCCTGTC
    GAGTGTTTGA CTCTGACCGT GGAGGAGGTG ATGCACATCA GACAGGTTCT TGTCAAAGCC
    GAACTGGAGA AGTTCCAGCA GTATAAAGAC ATTTATAATG CACTGAAGAA AGGAAAGCTT
    TGCTTCTCAT GTCGGTCGAA GAAGTTCTCA TTATTTACCT GGTCCTACAC CTGTCAGTTC
    TGTAAGAGGC CGGTTTGCTC ACAGTGCTGC AAGAAGATGA AATTGCCCTC CAAGCCTCAC
    GCCAGCCTGC CCATCTCCTC TTTGGGTCCC AGTATCCTGC CCAAAAAAGA GCCCGGAGCA
    TCATCAGCCC CGACAGATAA AACCTCCTCC ACATCCAGCC ACAAGAAAAA CAGCCTTCAG
    CGCTCATTGT CTAGATCATC AAAACACGGC GATCGCTCGT CTTCTAAAGA CGAGCTGGAG
    CTTCCGGAGC AGTTCACCGA GGACTGGAGC ACCATGGAGG TCTGCGTGGA CTGCAAAAAA
    TTCATCAACG ACATCATCAG CAACAGCCGC CGCAACCTGT CCACCAAACG TGCCCGCCTG
    CACCGCCGGA CACACTCCGT CTACTCGTCC AGCACCAGCT CCTCCAACTA CAAACCAACC
    GAGCGCACCA TCAAAGAGGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159678.1
    CDS210..2513
    Translation

    Target ORF information:

    RefSeq Version XM_005159621.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159621.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATAAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCAGATGG ACCTCGCAAG TTGGCCATCA GCACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAAG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGATGTCT
    TCAGACGGCA AGGCTCACGC ACTAGGCAGC GGTCATGCGG AGGAGTTCTG CTTCCCTGTC
    GAGTGTTTGA CTCTGACCGT GGAGGAGGTG ATGCACATCA GACAGGTTCT TGTCAAAGCC
    GAACTGGAGA AGTTCCAGCA GTATAAAGAC ATTTATAATG CACTGAAGAA AGGAAAGCTT
    TGCTTCTCAT GTCGGTCGAA GAAGTTCTCA TTATTTACCT GGTCCTACAC CTGTCAGTTC
    TGTAAGAGGC CGGTTTGCTC ACAGTGCTGC AAGAAGATGA AATTGCCCTC CAAGCCTCAC
    GCCAGCCTGC CCATCTCCTC TTTGGGTCCC AGTATCCTGC CCAAAAAAGA GCCCGGAGCA
    TCATCAGCCC CGACAGATAA AACCTCCTCC ACATCCAGCC ACAAGAAAAA CAGCCTTCAG
    CGCTCATTGT CTAGATCATC AAAACACGGC GATCGCTCGT CTTCTAAAGA CGAGCTGGAG
    CTTCCGGAGC AGTTCACCGA GGACTGGAGC ACCATGGAGG TCTGCGTGGA CTGCAAAAAA
    TTCATCAACG ACATCATCAG CAACAGCCGC CGCAACCTGT CCACCAAACG TGCCCGCCTG
    CACCGCCGGA CACACTCCGT CTACTCGTCC AGCACCAGCT CCTCCAACTA CAAACCAACC
    GAGCGCACCA TCAAAGAGGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa24281
    Clone ID Related Accession (Same CDS sequence) XM_005159622.3 , XM_005159622.4
    Accession Version XM_005159622.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein spire homolog 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATAAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCAGATGG ACCTCGCAAG TTGGCCATCA GCACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAAG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACATCATC
    AGCAACAGCC GCCGCAACCT GTCCACCAAA CGTGCCCGCC TGCACCGCCG GACACACTCC
    GTCTACTCGT CCAGCACCAG CTCCTCCAAC TACAAACCAA CCGAGCGCAC CATCAAAGAG
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159679.1
    CDS210..2495
    Translation

    Target ORF information:

    RefSeq Version XM_005159622.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159622.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATAAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCAGATGG ACCTCGCAAG TTGGCCATCA GCACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAAG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACATCATC
    AGCAACAGCC GCCGCAACCT GTCCACCAAA CGTGCCCGCC TGCACCGCCG GACACACTCC
    GTCTACTCGT CCAGCACCAG CTCCTCCAAC TACAAACCAA CCGAGCGCAC CATCAAAGAG
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa24281
    Clone ID Related Accession (Same CDS sequence) XM_005159622.3 , XM_005159622.4
    Accession Version XM_005159622.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159622.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATAAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCAGATGG ACCTCGCAAG TTGGCCATCA GCACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAAG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACATCATC
    AGCAACAGCC GCCGCAACCT GTCCACCAAA CGTGCCCGCC TGCACCGCCG GACACACTCC
    GTCTACTCGT CCAGCACCAG CTCCTCCAAC TACAAACCAA CCGAGCGCAC CATCAAAGAG
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159679.1
    CDS140..2425
    Translation

    Target ORF information:

    RefSeq Version XM_005159622.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159622.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGG CGCAGGCAAA AGTATAAGCA CTCCACAGGA CTTGTTCAGA
    AGCTCAGATA TCCCAGATGG ACCTCGCAAG TTGGCCATCA GCACACTATC TCTGGCTAAC
    GGCACGTCAC CGGCTCGAAG TCCTGTGAAC GGTGTAGCCG GAGGGCATTC ACTGTCCCAG
    AGGAAAAGGT TACTCAAAGC ACCTACACTA GCCGAACTGG ACAGCTCCGA CTCTGAGGAG
    GAGCAAAGCA CCCGCAAATC TGACAGCAGC TCCAGCATAT CCACCTCACT GGTTGAGGAC
    ACGTCTCCAG AGTCTGTCAT GGGCAAAAAA CCTCCACCTC AGTTCCTGCC CATTTCTTCC
    ACACCTCAGC CTGATAAACG CATCGCTCCT CAGAGGAGAC ACTCCATTGA GAAAGAGGCT
    CCCACCAACA TACGCCACTT CCTGCCTCCA TCTCGACAGA ACTCCAAATC TCTGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACATCATC
    AGCAACAGCC GCCGCAACCT GTCCACCAAA CGTGCCCGCC TGCACCGCCG GACACACTCC
    GTCTACTCGT CCAGCACCAG CTCCTCCAAC TACAAACCAA CCGAGCGCAC CATCAAAGAG
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa24283
    Clone ID Related Accession (Same CDS sequence) XM_005159624.4 , XM_005159624.3
    Accession Version XM_005159624.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159624.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGA TATCCCAGAT GGACCTCGCA AGTTGGCCAT CAGCACACTA
    TCTCTGGCTA ACGGCACGTC ACCGGCTCGA AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT
    TCACTGTCCC AGAGGAAAAG GTTACTCAAA GCACCTACAC TAGCCGAACT GGACAGCTCC
    GACTCTGAGG AGGAGCAAAG CACCCGCAAA TCTGACAGCA GCTCCAGCAT ATCCACCTCA
    CTGGTTGAGG ACACGTCTCC AGAGTCTGTC ATGGGCAAAA AACCTCCACC TCAGTTCCTG
    CCCATTTCTT CCACACCTCA GCCTGATAAA CGCATCGCTC CTCAGAGGAG ACACTCCATT
    GAGAAAGAGG CTCCCACCAA CATACGCCAC TTCCTGCCTC CATCTCGACA GAACTCCAAA
    TCTCTGATGT CTTCAGACGG CAAGGCTCAC GCACTAGGCA GCGGTCATGC GGAGGAGTTC
    TGCTTCCCTG TCGAGTGTTT GACTCTGACC GTGGAGGAGG TGATGCACAT CAGACAGGTT
    CTTGTCAAAG CCGAACTGGA GAAGTTCCAG CAGTATAAAG ACATTTATAA TGCACTGAAG
    AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG AAGAAGTTCT CATTATTTAC CTGGTCCTAC
    ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC TCACAGTGCT GCAAGAAGAT GAAATTGCCC
    TCCAAGCCTC ACGCCAGCCT GCCCATCTCC TCTTTGGGTC CCAGTATCCT GCCCAAAAAA
    GAGCCCGGAG CATCATCAGC CCCGACAGAT AAAACCTCCT CCACATCCAG CCACAAGAAA
    AACAGCCTTC AGCGCTCATT GTCTAGATCA TCAAAACACG GCGATCGCTC GTCTTCTAAA
    GACGAGCTGG AGCTTCCGGA GCAGTTCACC GAGGACTGGA GCACCATGGA GGTCTGCGTG
    GACTGCAAAA AATTCATCAA CGACATCATC AGCAACAGCC GCCGCAACCT GTCCACCAAA
    CGTGCCCGCC TGCACCGCCG GACACACTCC GTCTACTCGT CCAGCACCAG CTCCTCCAAC
    TACAAACCAA CCGAGCGCAC CATCAAAGAG GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159681.1
    CDS181..2436
    Translation

    Target ORF information:

    RefSeq Version XM_005159624.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159624.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGA TATCCCAGAT GGACCTCGCA AGTTGGCCAT CAGCACACTA
    TCTCTGGCTA ACGGCACGTC ACCGGCTCGA AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT
    TCACTGTCCC AGAGGAAAAG GTTACTCAAA GCACCTACAC TAGCCGAACT GGACAGCTCC
    GACTCTGAGG AGGAGCAAAG CACCCGCAAA TCTGACAGCA GCTCCAGCAT ATCCACCTCA
    CTGGTTGAGG ACACGTCTCC AGAGTCTGTC ATGGGCAAAA AACCTCCACC TCAGTTCCTG
    CCCATTTCTT CCACACCTCA GCCTGATAAA CGCATCGCTC CTCAGAGGAG ACACTCCATT
    GAGAAAGAGG CTCCCACCAA CATACGCCAC TTCCTGCCTC CATCTCGACA GAACTCCAAA
    TCTCTGATGT CTTCAGACGG CAAGGCTCAC GCACTAGGCA GCGGTCATGC GGAGGAGTTC
    TGCTTCCCTG TCGAGTGTTT GACTCTGACC GTGGAGGAGG TGATGCACAT CAGACAGGTT
    CTTGTCAAAG CCGAACTGGA GAAGTTCCAG CAGTATAAAG ACATTTATAA TGCACTGAAG
    AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG AAGAAGTTCT CATTATTTAC CTGGTCCTAC
    ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC TCACAGTGCT GCAAGAAGAT GAAATTGCCC
    TCCAAGCCTC ACGCCAGCCT GCCCATCTCC TCTTTGGGTC CCAGTATCCT GCCCAAAAAA
    GAGCCCGGAG CATCATCAGC CCCGACAGAT AAAACCTCCT CCACATCCAG CCACAAGAAA
    AACAGCCTTC AGCGCTCATT GTCTAGATCA TCAAAACACG GCGATCGCTC GTCTTCTAAA
    GACGAGCTGG AGCTTCCGGA GCAGTTCACC GAGGACTGGA GCACCATGGA GGTCTGCGTG
    GACTGCAAAA AATTCATCAA CGACATCATC AGCAACAGCC GCCGCAACCT GTCCACCAAA
    CGTGCCCGCC TGCACCGCCG GACACACTCC GTCTACTCGT CCAGCACCAG CTCCTCCAAC
    TACAAACCAA CCGAGCGCAC CATCAAAGAG GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa24283
    Clone ID Related Accession (Same CDS sequence) XM_005159624.4 , XM_005159624.3
    Accession Version XM_005159624.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein spire homolog 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159624.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGA TATCCCAGAT GGACCTCGCA AGTTGGCCAT CAGCACACTA
    TCTCTGGCTA ACGGCACGTC ACCGGCTCGA AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT
    TCACTGTCCC AGAGGAAAAG GTTACTCAAA GCACCTACAC TAGCCGAACT GGACAGCTCC
    GACTCTGAGG AGGAGCAAAG CACCCGCAAA TCTGACAGCA GCTCCAGCAT ATCCACCTCA
    CTGGTTGAGG ACACGTCTCC AGAGTCTGTC ATGGGCAAAA AACCTCCACC TCAGTTCCTG
    CCCATTTCTT CCACACCTCA GCCTGATAAA CGCATCGCTC CTCAGAGGAG ACACTCCATT
    GAGAAAGAGG CTCCCACCAA CATACGCCAC TTCCTGCCTC CATCTCGACA GAACTCCAAA
    TCTCTGATGT CTTCAGACGG CAAGGCTCAC GCACTAGGCA GCGGTCATGC GGAGGAGTTC
    TGCTTCCCTG TCGAGTGTTT GACTCTGACC GTGGAGGAGG TGATGCACAT CAGACAGGTT
    CTTGTCAAAG CCGAACTGGA GAAGTTCCAG CAGTATAAAG ACATTTATAA TGCACTGAAG
    AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG AAGAAGTTCT CATTATTTAC CTGGTCCTAC
    ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC TCACAGTGCT GCAAGAAGAT GAAATTGCCC
    TCCAAGCCTC ACGCCAGCCT GCCCATCTCC TCTTTGGGTC CCAGTATCCT GCCCAAAAAA
    GAGCCCGGAG CATCATCAGC CCCGACAGAT AAAACCTCCT CCACATCCAG CCACAAGAAA
    AACAGCCTTC AGCGCTCATT GTCTAGATCA TCAAAACACG GCGATCGCTC GTCTTCTAAA
    GACGAGCTGG AGCTTCCGGA GCAGTTCACC GAGGACTGGA GCACCATGGA GGTCTGCGTG
    GACTGCAAAA AATTCATCAA CGACATCATC AGCAACAGCC GCCGCAACCT GTCCACCAAA
    CGTGCCCGCC TGCACCGCCG GACACACTCC GTCTACTCGT CCAGCACCAG CTCCTCCAAC
    TACAAACCAA CCGAGCGCAC CATCAAAGAG GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159681.1
    CDS210..2465
    Translation

    Target ORF information:

    RefSeq Version XM_005159624.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159624.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGA TATCCCAGAT GGACCTCGCA AGTTGGCCAT CAGCACACTA
    TCTCTGGCTA ACGGCACGTC ACCGGCTCGA AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT
    TCACTGTCCC AGAGGAAAAG GTTACTCAAA GCACCTACAC TAGCCGAACT GGACAGCTCC
    GACTCTGAGG AGGAGCAAAG CACCCGCAAA TCTGACAGCA GCTCCAGCAT ATCCACCTCA
    CTGGTTGAGG ACACGTCTCC AGAGTCTGTC ATGGGCAAAA AACCTCCACC TCAGTTCCTG
    CCCATTTCTT CCACACCTCA GCCTGATAAA CGCATCGCTC CTCAGAGGAG ACACTCCATT
    GAGAAAGAGG CTCCCACCAA CATACGCCAC TTCCTGCCTC CATCTCGACA GAACTCCAAA
    TCTCTGATGT CTTCAGACGG CAAGGCTCAC GCACTAGGCA GCGGTCATGC GGAGGAGTTC
    TGCTTCCCTG TCGAGTGTTT GACTCTGACC GTGGAGGAGG TGATGCACAT CAGACAGGTT
    CTTGTCAAAG CCGAACTGGA GAAGTTCCAG CAGTATAAAG ACATTTATAA TGCACTGAAG
    AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG AAGAAGTTCT CATTATTTAC CTGGTCCTAC
    ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC TCACAGTGCT GCAAGAAGAT GAAATTGCCC
    TCCAAGCCTC ACGCCAGCCT GCCCATCTCC TCTTTGGGTC CCAGTATCCT GCCCAAAAAA
    GAGCCCGGAG CATCATCAGC CCCGACAGAT AAAACCTCCT CCACATCCAG CCACAAGAAA
    AACAGCCTTC AGCGCTCATT GTCTAGATCA TCAAAACACG GCGATCGCTC GTCTTCTAAA
    GACGAGCTGG AGCTTCCGGA GCAGTTCACC GAGGACTGGA GCACCATGGA GGTCTGCGTG
    GACTGCAAAA AATTCATCAA CGACATCATC AGCAACAGCC GCCGCAACCT GTCCACCAAA
    CGTGCCCGCC TGCACCGCCG GACACACTCC GTCTACTCGT CCAGCACCAG CTCCTCCAAC
    TACAAACCAA CCGAGCGCAC CATCAAAGAG GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42704
    Clone ID Related Accession (Same CDS sequence) XM_009294320.2 , XM_009294320.3
    Accession Version XM_009294320.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein spire homolog 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGATGT CTTCAGACGG CAAGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACATCATC
    AGCAACAGCC GCCGCAACCT GTCCACCAAA CGTGCCCGCC TGCACCGCCG GACACACTCC
    GTCTACTCGT CCAGCACCAG CTCCTCCAAC TACAAACCAA CCGAGCGCAC CATCAAAGAG
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292595.1
    CDS210..2555
    Translation

    Target ORF information:

    RefSeq Version XM_009294320.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294320.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGATGT CTTCAGACGG CAAGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACATCATC
    AGCAACAGCC GCCGCAACCT GTCCACCAAA CGTGCCCGCC TGCACCGCCG GACACACTCC
    GTCTACTCGT CCAGCACCAG CTCCTCCAAC TACAAACCAA CCGAGCGCAC CATCAAAGAG
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42704
    Clone ID Related Accession (Same CDS sequence) XM_009294320.2 , XM_009294320.3
    Accession Version XM_009294320.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294320.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGATGT CTTCAGACGG CAAGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACATCATC
    AGCAACAGCC GCCGCAACCT GTCCACCAAA CGTGCCCGCC TGCACCGCCG GACACACTCC
    GTCTACTCGT CCAGCACCAG CTCCTCCAAC TACAAACCAA CCGAGCGCAC CATCAAAGAG
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292595.1
    CDS182..2527
    Translation

    Target ORF information:

    RefSeq Version XM_009294320.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294320.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGATGT CTTCAGACGG CAAGGCTCAC
    GCACTAGGCA GCGGTCATGC GGAGGAGTTC TGCTTCCCTG TCGAGTGTTT GACTCTGACC
    GTGGAGGAGG TGATGCACAT CAGACAGGTT CTTGTCAAAG CCGAACTGGA GAAGTTCCAG
    CAGTATAAAG ACATTTATAA TGCACTGAAG AAAGGAAAGC TTTGCTTCTC ATGTCGGTCG
    AAGAAGTTCT CATTATTTAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCGGTTTGC
    TCACAGTGCT GCAAGAAGAT GAAATTGCCC TCCAAGCCTC ACGCCAGCCT GCCCATCTCC
    TCTTTGGGTC CCAGTATCCT GCCCAAAAAA GAGCCCGGAG CATCATCAGC CCCGACAGAT
    AAAACCTCCT CCACATCCAG CCACAAGAAA AACAGCCTTC AGCGCTCATT GTCTAGATCA
    TCAAAACACG GCGATCGCTC GTCTTCTAAA GACGAGCTGG AGCTTCCGGA GCAGTTCACC
    GAGGACTGGA GCACCATGGA GGTCTGCGTG GACTGCAAAA AATTCATCAA CGACATCATC
    AGCAACAGCC GCCGCAACCT GTCCACCAAA CGTGCCCGCC TGCACCGCCG GACACACTCC
    GTCTACTCGT CCAGCACCAG CTCCTCCAAC TACAAACCAA CCGAGCGCAC CATCAAAGAG
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42705
    Clone ID Related Accession (Same CDS sequence) XM_009294321.3 , XM_009294321.2
    Accession Version XM_009294321.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGGCTC ACGCACTAGG CAGCGGTCAT
    GCGGAGGAGT TCTGCTTCCC TGTCGAGTGT TTGACTCTGA CCGTGGAGGA GGTGATGCAC
    ATCAGACAGG TTCTTGTCAA AGCCGAACTG GAGAAGTTCC AGCAGTATAA AGACATTTAT
    AATGCACTGA AGAAAGGAAA GCTTTGCTTC TCATGTCGGT CGAAGAAGTT CTCATTATTT
    ACCTGGTCCT ACACCTGTCA GTTCTGTAAG AGGCCGGTTT GCTCACAGTG CTGCAAGAAG
    ATGAAATTGC CCTCCAAGCC TCACGCCAGC CTGCCCATCT CCTCTTTGGG TCCCAGTATC
    CTGCCCAAAA AAGAGCCCGG AGCATCATCA GCCCCGACAG ATAAAACCTC CTCCACATCC
    AGCCACAAGA AAAACAGCCT TCAGCGCTCA TTGTCTAGAT CATCAAAACA CGGCGATCGC
    TCGTCTTCTA AAGACGAGCT GGAGCTTCCG GAGCAGTTCA CCGAGGACTG GAGCACCATG
    GAGGTCTGCG TGGACTGCAA AAAATTCATC AACGACATCA TCAGCAACAG CCGCCGCAAC
    CTGTCCACCA AACGTGCCCG CCTGCACCGC CGGACACACT CCGTCTACTC GTCCAGCACC
    AGCTCCTCCA ACTACAAACC AACCGAGCGC ACCATCAAAG AGGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292596.1
    CDS182..2509
    Translation

    Target ORF information:

    RefSeq Version XM_009294321.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294321.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGGCTC ACGCACTAGG CAGCGGTCAT
    GCGGAGGAGT TCTGCTTCCC TGTCGAGTGT TTGACTCTGA CCGTGGAGGA GGTGATGCAC
    ATCAGACAGG TTCTTGTCAA AGCCGAACTG GAGAAGTTCC AGCAGTATAA AGACATTTAT
    AATGCACTGA AGAAAGGAAA GCTTTGCTTC TCATGTCGGT CGAAGAAGTT CTCATTATTT
    ACCTGGTCCT ACACCTGTCA GTTCTGTAAG AGGCCGGTTT GCTCACAGTG CTGCAAGAAG
    ATGAAATTGC CCTCCAAGCC TCACGCCAGC CTGCCCATCT CCTCTTTGGG TCCCAGTATC
    CTGCCCAAAA AAGAGCCCGG AGCATCATCA GCCCCGACAG ATAAAACCTC CTCCACATCC
    AGCCACAAGA AAAACAGCCT TCAGCGCTCA TTGTCTAGAT CATCAAAACA CGGCGATCGC
    TCGTCTTCTA AAGACGAGCT GGAGCTTCCG GAGCAGTTCA CCGAGGACTG GAGCACCATG
    GAGGTCTGCG TGGACTGCAA AAAATTCATC AACGACATCA TCAGCAACAG CCGCCGCAAC
    CTGTCCACCA AACGTGCCCG CCTGCACCGC CGGACACACT CCGTCTACTC GTCCAGCACC
    AGCTCCTCCA ACTACAAACC AACCGAGCGC ACCATCAAAG AGGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42705
    Clone ID Related Accession (Same CDS sequence) XM_009294321.3 , XM_009294321.2
    Accession Version XM_009294321.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein spire homolog 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGGCTC ACGCACTAGG CAGCGGTCAT
    GCGGAGGAGT TCTGCTTCCC TGTCGAGTGT TTGACTCTGA CCGTGGAGGA GGTGATGCAC
    ATCAGACAGG TTCTTGTCAA AGCCGAACTG GAGAAGTTCC AGCAGTATAA AGACATTTAT
    AATGCACTGA AGAAAGGAAA GCTTTGCTTC TCATGTCGGT CGAAGAAGTT CTCATTATTT
    ACCTGGTCCT ACACCTGTCA GTTCTGTAAG AGGCCGGTTT GCTCACAGTG CTGCAAGAAG
    ATGAAATTGC CCTCCAAGCC TCACGCCAGC CTGCCCATCT CCTCTTTGGG TCCCAGTATC
    CTGCCCAAAA AAGAGCCCGG AGCATCATCA GCCCCGACAG ATAAAACCTC CTCCACATCC
    AGCCACAAGA AAAACAGCCT TCAGCGCTCA TTGTCTAGAT CATCAAAACA CGGCGATCGC
    TCGTCTTCTA AAGACGAGCT GGAGCTTCCG GAGCAGTTCA CCGAGGACTG GAGCACCATG
    GAGGTCTGCG TGGACTGCAA AAAATTCATC AACGACATCA TCAGCAACAG CCGCCGCAAC
    CTGTCCACCA AACGTGCCCG CCTGCACCGC CGGACACACT CCGTCTACTC GTCCAGCACC
    AGCTCCTCCA ACTACAAACC AACCGAGCGC ACCATCAAAG AGGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292596.1
    CDS210..2537
    Translation

    Target ORF information:

    RefSeq Version XM_009294321.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294321.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGGCTC ACGCACTAGG CAGCGGTCAT
    GCGGAGGAGT TCTGCTTCCC TGTCGAGTGT TTGACTCTGA CCGTGGAGGA GGTGATGCAC
    ATCAGACAGG TTCTTGTCAA AGCCGAACTG GAGAAGTTCC AGCAGTATAA AGACATTTAT
    AATGCACTGA AGAAAGGAAA GCTTTGCTTC TCATGTCGGT CGAAGAAGTT CTCATTATTT
    ACCTGGTCCT ACACCTGTCA GTTCTGTAAG AGGCCGGTTT GCTCACAGTG CTGCAAGAAG
    ATGAAATTGC CCTCCAAGCC TCACGCCAGC CTGCCCATCT CCTCTTTGGG TCCCAGTATC
    CTGCCCAAAA AAGAGCCCGG AGCATCATCA GCCCCGACAG ATAAAACCTC CTCCACATCC
    AGCCACAAGA AAAACAGCCT TCAGCGCTCA TTGTCTAGAT CATCAAAACA CGGCGATCGC
    TCGTCTTCTA AAGACGAGCT GGAGCTTCCG GAGCAGTTCA CCGAGGACTG GAGCACCATG
    GAGGTCTGCG TGGACTGCAA AAAATTCATC AACGACATCA TCAGCAACAG CCGCCGCAAC
    CTGTCCACCA AACGTGCCCG CCTGCACCGC CGGACACACT CCGTCTACTC GTCCAGCACC
    AGCTCCTCCA ACTACAAACC AACCGAGCGC ACCATCAAAG AGGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42706
    Clone ID Related Accession (Same CDS sequence) XM_009294322.3 , XM_009294322.2
    Accession Version XM_009294322.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294322.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGATGT CTTCAGACGG CAAGGAGGAG
    TTCTGCTTCC CTGTCGAGTG TTTGACTCTG ACCGTGGAGG AGGTGATGCA CATCAGACAG
    GTTCTTGTCA AAGCCGAACT GGAGAAGTTC CAGCAGTATA AAGACATTTA TAATGCACTG
    AAGAAAGGAA AGCTTTGCTT CTCATGTCGG TCGAAGAAGT TCTCATTATT TACCTGGTCC
    TACACCTGTC AGTTCTGTAA GAGGCCGGTT TGCTCACAGT GCTGCAAGAA GATGAAATTG
    CCCTCCAAGC CTCACGCCAG CCTGCCCATC TCCTCTTTGG GTCCCAGTAT CCTGCCCAAA
    AAAGAGCCCG GAGCATCATC AGCCCCGACA GATAAAACCT CCTCCACATC CAGCCACAAG
    AAAAACAGCC TTCAGCGCTC ATTGTCTAGA TCATCAAAAC ACGGCGATCG CTCGTCTTCT
    AAAGACGAGC TGGAGCTTCC GGAGCAGTTC ACCGAGGACT GGAGCACCAT GGAGGTCTGC
    GTGGACTGCA AAAAATTCAT CAACGACATC ATCAGCAACA GCCGCCGCAA CCTGTCCACC
    AAACGTGCCC GCCTGCACCG CCGGACACAC TCCGTCTACT CGTCCAGCAC CAGCTCCTCC
    AACTACAAAC CAACCGAGCG CACCATCAAA GAGGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292597.1
    CDS182..2500
    Translation

    Target ORF information:

    RefSeq Version XM_009294322.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294322.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGATGT CTTCAGACGG CAAGGAGGAG
    TTCTGCTTCC CTGTCGAGTG TTTGACTCTG ACCGTGGAGG AGGTGATGCA CATCAGACAG
    GTTCTTGTCA AAGCCGAACT GGAGAAGTTC CAGCAGTATA AAGACATTTA TAATGCACTG
    AAGAAAGGAA AGCTTTGCTT CTCATGTCGG TCGAAGAAGT TCTCATTATT TACCTGGTCC
    TACACCTGTC AGTTCTGTAA GAGGCCGGTT TGCTCACAGT GCTGCAAGAA GATGAAATTG
    CCCTCCAAGC CTCACGCCAG CCTGCCCATC TCCTCTTTGG GTCCCAGTAT CCTGCCCAAA
    AAAGAGCCCG GAGCATCATC AGCCCCGACA GATAAAACCT CCTCCACATC CAGCCACAAG
    AAAAACAGCC TTCAGCGCTC ATTGTCTAGA TCATCAAAAC ACGGCGATCG CTCGTCTTCT
    AAAGACGAGC TGGAGCTTCC GGAGCAGTTC ACCGAGGACT GGAGCACCAT GGAGGTCTGC
    GTGGACTGCA AAAAATTCAT CAACGACATC ATCAGCAACA GCCGCCGCAA CCTGTCCACC
    AAACGTGCCC GCCTGCACCG CCGGACACAC TCCGTCTACT CGTCCAGCAC CAGCTCCTCC
    AACTACAAAC CAACCGAGCG CACCATCAAA GAGGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42706
    Clone ID Related Accession (Same CDS sequence) XM_009294322.3 , XM_009294322.2
    Accession Version XM_009294322.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein spire homolog 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGATGT CTTCAGACGG CAAGGAGGAG
    TTCTGCTTCC CTGTCGAGTG TTTGACTCTG ACCGTGGAGG AGGTGATGCA CATCAGACAG
    GTTCTTGTCA AAGCCGAACT GGAGAAGTTC CAGCAGTATA AAGACATTTA TAATGCACTG
    AAGAAAGGAA AGCTTTGCTT CTCATGTCGG TCGAAGAAGT TCTCATTATT TACCTGGTCC
    TACACCTGTC AGTTCTGTAA GAGGCCGGTT TGCTCACAGT GCTGCAAGAA GATGAAATTG
    CCCTCCAAGC CTCACGCCAG CCTGCCCATC TCCTCTTTGG GTCCCAGTAT CCTGCCCAAA
    AAAGAGCCCG GAGCATCATC AGCCCCGACA GATAAAACCT CCTCCACATC CAGCCACAAG
    AAAAACAGCC TTCAGCGCTC ATTGTCTAGA TCATCAAAAC ACGGCGATCG CTCGTCTTCT
    AAAGACGAGC TGGAGCTTCC GGAGCAGTTC ACCGAGGACT GGAGCACCAT GGAGGTCTGC
    GTGGACTGCA AAAAATTCAT CAACGACATC ATCAGCAACA GCCGCCGCAA CCTGTCCACC
    AAACGTGCCC GCCTGCACCG CCGGACACAC TCCGTCTACT CGTCCAGCAC CAGCTCCTCC
    AACTACAAAC CAACCGAGCG CACCATCAAA GAGGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292597.1
    CDS210..2528
    Translation

    Target ORF information:

    RefSeq Version XM_009294322.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294322.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGATGT CTTCAGACGG CAAGGAGGAG
    TTCTGCTTCC CTGTCGAGTG TTTGACTCTG ACCGTGGAGG AGGTGATGCA CATCAGACAG
    GTTCTTGTCA AAGCCGAACT GGAGAAGTTC CAGCAGTATA AAGACATTTA TAATGCACTG
    AAGAAAGGAA AGCTTTGCTT CTCATGTCGG TCGAAGAAGT TCTCATTATT TACCTGGTCC
    TACACCTGTC AGTTCTGTAA GAGGCCGGTT TGCTCACAGT GCTGCAAGAA GATGAAATTG
    CCCTCCAAGC CTCACGCCAG CCTGCCCATC TCCTCTTTGG GTCCCAGTAT CCTGCCCAAA
    AAAGAGCCCG GAGCATCATC AGCCCCGACA GATAAAACCT CCTCCACATC CAGCCACAAG
    AAAAACAGCC TTCAGCGCTC ATTGTCTAGA TCATCAAAAC ACGGCGATCG CTCGTCTTCT
    AAAGACGAGC TGGAGCTTCC GGAGCAGTTC ACCGAGGACT GGAGCACCAT GGAGGTCTGC
    GTGGACTGCA AAAAATTCAT CAACGACATC ATCAGCAACA GCCGCCGCAA CCTGTCCACC
    AAACGTGCCC GCCTGCACCG CCGGACACAC TCCGTCTACT CGTCCAGCAC CAGCTCCTCC
    AACTACAAAC CAACCGAGCG CACCATCAAA GAGGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42707
    Clone ID Related Accession (Same CDS sequence) XM_009294323.2 , XM_009294323.3
    Accession Version XM_009294323.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein spire homolog 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGGAGG AGTTCTGCTT CCCTGTCGAG
    TGTTTGACTC TGACCGTGGA GGAGGTGATG CACATCAGAC AGGTTCTTGT CAAAGCCGAA
    CTGGAGAAGT TCCAGCAGTA TAAAGACATT TATAATGCAC TGAAGAAAGG AAAGCTTTGC
    TTCTCATGTC GGTCGAAGAA GTTCTCATTA TTTACCTGGT CCTACACCTG TCAGTTCTGT
    AAGAGGCCGG TTTGCTCACA GTGCTGCAAG AAGATGAAAT TGCCCTCCAA GCCTCACGCC
    AGCCTGCCCA TCTCCTCTTT GGGTCCCAGT ATCCTGCCCA AAAAAGAGCC CGGAGCATCA
    TCAGCCCCGA CAGATAAAAC CTCCTCCACA TCCAGCCACA AGAAAAACAG CCTTCAGCGC
    TCATTGTCTA GATCATCAAA ACACGGCGAT CGCTCGTCTT CTAAAGACGA GCTGGAGCTT
    CCGGAGCAGT TCACCGAGGA CTGGAGCACC ATGGAGGTCT GCGTGGACTG CAAAAAATTC
    ATCAACGACA TCATCAGCAA CAGCCGCCGC AACCTGTCCA CCAAACGTGC CCGCCTGCAC
    CGCCGGACAC ACTCCGTCTA CTCGTCCAGC ACCAGCTCCT CCAACTACAA ACCAACCGAG
    CGCACCATCA AAGAGGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292598.1
    CDS210..2510
    Translation

    Target ORF information:

    RefSeq Version XM_009294323.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294323.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGGAGG AGTTCTGCTT CCCTGTCGAG
    TGTTTGACTC TGACCGTGGA GGAGGTGATG CACATCAGAC AGGTTCTTGT CAAAGCCGAA
    CTGGAGAAGT TCCAGCAGTA TAAAGACATT TATAATGCAC TGAAGAAAGG AAAGCTTTGC
    TTCTCATGTC GGTCGAAGAA GTTCTCATTA TTTACCTGGT CCTACACCTG TCAGTTCTGT
    AAGAGGCCGG TTTGCTCACA GTGCTGCAAG AAGATGAAAT TGCCCTCCAA GCCTCACGCC
    AGCCTGCCCA TCTCCTCTTT GGGTCCCAGT ATCCTGCCCA AAAAAGAGCC CGGAGCATCA
    TCAGCCCCGA CAGATAAAAC CTCCTCCACA TCCAGCCACA AGAAAAACAG CCTTCAGCGC
    TCATTGTCTA GATCATCAAA ACACGGCGAT CGCTCGTCTT CTAAAGACGA GCTGGAGCTT
    CCGGAGCAGT TCACCGAGGA CTGGAGCACC ATGGAGGTCT GCGTGGACTG CAAAAAATTC
    ATCAACGACA TCATCAGCAA CAGCCGCCGC AACCTGTCCA CCAAACGTGC CCGCCTGCAC
    CGCCGGACAC ACTCCGTCTA CTCGTCCAGC ACCAGCTCCT CCAACTACAA ACCAACCGAG
    CGCACCATCA AAGAGGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42707
    Clone ID Related Accession (Same CDS sequence) XM_009294323.2 , XM_009294323.3
    Accession Version XM_009294323.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294323.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGGAGG AGTTCTGCTT CCCTGTCGAG
    TGTTTGACTC TGACCGTGGA GGAGGTGATG CACATCAGAC AGGTTCTTGT CAAAGCCGAA
    CTGGAGAAGT TCCAGCAGTA TAAAGACATT TATAATGCAC TGAAGAAAGG AAAGCTTTGC
    TTCTCATGTC GGTCGAAGAA GTTCTCATTA TTTACCTGGT CCTACACCTG TCAGTTCTGT
    AAGAGGCCGG TTTGCTCACA GTGCTGCAAG AAGATGAAAT TGCCCTCCAA GCCTCACGCC
    AGCCTGCCCA TCTCCTCTTT GGGTCCCAGT ATCCTGCCCA AAAAAGAGCC CGGAGCATCA
    TCAGCCCCGA CAGATAAAAC CTCCTCCACA TCCAGCCACA AGAAAAACAG CCTTCAGCGC
    TCATTGTCTA GATCATCAAA ACACGGCGAT CGCTCGTCTT CTAAAGACGA GCTGGAGCTT
    CCGGAGCAGT TCACCGAGGA CTGGAGCACC ATGGAGGTCT GCGTGGACTG CAAAAAATTC
    ATCAACGACA TCATCAGCAA CAGCCGCCGC AACCTGTCCA CCAAACGTGC CCGCCTGCAC
    CGCCGGACAC ACTCCGTCTA CTCGTCCAGC ACCAGCTCCT CCAACTACAA ACCAACCGAG
    CGCACCATCA AAGAGGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292598.1
    CDS182..2482
    Translation

    Target ORF information:

    RefSeq Version XM_009294323.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294323.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGT AATGCGTCCT CTTAGCATGT CTGTCAGTTT TGACTCATCA
    GGCGCAGGCA AAAGTATAAG CACTCCACAG GACTTGTTCA GAAGCTCAGA TATCCCAGAT
    GGACCTCGCA AGTTGGCCAT CAGCACACTA TCTCTGGCTA ACGGCACGTC ACCGGCTCGA
    AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT TCACTGTCCC AGAGGAAAAG GTTACTCAAA
    GCACCTACAC TAGCCGAACT GGACAGCTCC GACTCTGAGG AGGAGCAAAG CACCCGCAAA
    TCTGACAGCA GCTCCAGCAT ATCCACCTCA CTGGTTGAGG ACACGTCTCC AGAGTCTGTC
    ATGGGCAAAA AACCTCCACC TCAGTTCCTG CCCATTTCTT CCACACCTCA GCCTGATAAA
    CGCATCGCTC CTCAGAGGAG ACACTCCATT GAGAAAGAGG CTCCCACCAA CATACGCCAC
    TTCCTGCCTC CATCTCGACA GAACTCCAAA TCTCTGGAGG AGTTCTGCTT CCCTGTCGAG
    TGTTTGACTC TGACCGTGGA GGAGGTGATG CACATCAGAC AGGTTCTTGT CAAAGCCGAA
    CTGGAGAAGT TCCAGCAGTA TAAAGACATT TATAATGCAC TGAAGAAAGG AAAGCTTTGC
    TTCTCATGTC GGTCGAAGAA GTTCTCATTA TTTACCTGGT CCTACACCTG TCAGTTCTGT
    AAGAGGCCGG TTTGCTCACA GTGCTGCAAG AAGATGAAAT TGCCCTCCAA GCCTCACGCC
    AGCCTGCCCA TCTCCTCTTT GGGTCCCAGT ATCCTGCCCA AAAAAGAGCC CGGAGCATCA
    TCAGCCCCGA CAGATAAAAC CTCCTCCACA TCCAGCCACA AGAAAAACAG CCTTCAGCGC
    TCATTGTCTA GATCATCAAA ACACGGCGAT CGCTCGTCTT CTAAAGACGA GCTGGAGCTT
    CCGGAGCAGT TCACCGAGGA CTGGAGCACC ATGGAGGTCT GCGTGGACTG CAAAAAATTC
    ATCAACGACA TCATCAGCAA CAGCCGCCGC AACCTGTCCA CCAAACGTGC CCGCCTGCAC
    CGCCGGACAC ACTCCGTCTA CTCGTCCAGC ACCAGCTCCT CCAACTACAA ACCAACCGAG
    CGCACCATCA AAGAGGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42708
    Clone ID Related Accession (Same CDS sequence) XM_009294324.2 , XM_009294324.3
    Accession Version XM_009294324.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein spire homolog 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGA TATCCCAGAT GGACCTCGCA AGTTGGCCAT CAGCACACTA
    TCTCTGGCTA ACGGCACGTC ACCGGCTCGA AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT
    TCACTGTCCC AGAGGAAAAG GTTACTCAAA GCACCTACAC TAGCCGAACT GGACAGCTCC
    GACTCTGAGG AGGAGCAAAG CACCCGCAAA TCTGACAGCA GCTCCAGCAT ATCCACCTCA
    CTGGTTGAGG ACACGTCTCC AGAGTCTGTC ATGGGCAAAA AACCTCCACC TCAGTTCCTG
    CCCATTTCTT CCACACCTCA GCCTGATAAA CGCATCGCTC CTCAGAGGAG ACACTCCATT
    GAGAAAGAGG CTCCCACCAA CATACGCCAC TTCCTGCCTC CATCTCGACA GAACTCCAAA
    TCTCTGGAGG AGTTCTGCTT CCCTGTCGAG TGTTTGACTC TGACCGTGGA GGAGGTGATG
    CACATCAGAC AGGTTCTTGT CAAAGCCGAA CTGGAGAAGT TCCAGCAGTA TAAAGACATT
    TATAATGCAC TGAAGAAAGG AAAGCTTTGC TTCTCATGTC GGTCGAAGAA GTTCTCATTA
    TTTACCTGGT CCTACACCTG TCAGTTCTGT AAGAGGCCGG TTTGCTCACA GTGCTGCAAG
    AAGATGAAAT TGCCCTCCAA GCCTCACGCC AGCCTGCCCA TCTCCTCTTT GGGTCCCAGT
    ATCCTGCCCA AAAAAGAGCC CGGAGCATCA TCAGCCCCGA CAGATAAAAC CTCCTCCACA
    TCCAGCCACA AGAAAAACAG CCTTCAGCGC TCATTGTCTA GATCATCAAA ACACGGCGAT
    CGCTCGTCTT CTAAAGACGA GCTGGAGCTT CCGGAGCAGT TCACCGAGGA CTGGAGCACC
    ATGGAGGTCT GCGTGGACTG CAAAAAATTC ATCAACGACA TCATCAGCAA CAGCCGCCGC
    AACCTGTCCA CCAAACGTGC CCGCCTGCAC CGCCGGACAC ACTCCGTCTA CTCGTCCAGC
    ACCAGCTCCT CCAACTACAA ACCAACCGAG CGCACCATCA AAGAGGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292599.1
    CDS209..2419
    Translation

    Target ORF information:

    RefSeq Version XM_009294324.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294324.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGA TATCCCAGAT GGACCTCGCA AGTTGGCCAT CAGCACACTA
    TCTCTGGCTA ACGGCACGTC ACCGGCTCGA AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT
    TCACTGTCCC AGAGGAAAAG GTTACTCAAA GCACCTACAC TAGCCGAACT GGACAGCTCC
    GACTCTGAGG AGGAGCAAAG CACCCGCAAA TCTGACAGCA GCTCCAGCAT ATCCACCTCA
    CTGGTTGAGG ACACGTCTCC AGAGTCTGTC ATGGGCAAAA AACCTCCACC TCAGTTCCTG
    CCCATTTCTT CCACACCTCA GCCTGATAAA CGCATCGCTC CTCAGAGGAG ACACTCCATT
    GAGAAAGAGG CTCCCACCAA CATACGCCAC TTCCTGCCTC CATCTCGACA GAACTCCAAA
    TCTCTGGAGG AGTTCTGCTT CCCTGTCGAG TGTTTGACTC TGACCGTGGA GGAGGTGATG
    CACATCAGAC AGGTTCTTGT CAAAGCCGAA CTGGAGAAGT TCCAGCAGTA TAAAGACATT
    TATAATGCAC TGAAGAAAGG AAAGCTTTGC TTCTCATGTC GGTCGAAGAA GTTCTCATTA
    TTTACCTGGT CCTACACCTG TCAGTTCTGT AAGAGGCCGG TTTGCTCACA GTGCTGCAAG
    AAGATGAAAT TGCCCTCCAA GCCTCACGCC AGCCTGCCCA TCTCCTCTTT GGGTCCCAGT
    ATCCTGCCCA AAAAAGAGCC CGGAGCATCA TCAGCCCCGA CAGATAAAAC CTCCTCCACA
    TCCAGCCACA AGAAAAACAG CCTTCAGCGC TCATTGTCTA GATCATCAAA ACACGGCGAT
    CGCTCGTCTT CTAAAGACGA GCTGGAGCTT CCGGAGCAGT TCACCGAGGA CTGGAGCACC
    ATGGAGGTCT GCGTGGACTG CAAAAAATTC ATCAACGACA TCATCAGCAA CAGCCGCCGC
    AACCTGTCCA CCAAACGTGC CCGCCTGCAC CGCCGGACAC ACTCCGTCTA CTCGTCCAGC
    ACCAGCTCCT CCAACTACAA ACCAACCGAG CGCACCATCA AAGAGGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42708
    Clone ID Related Accession (Same CDS sequence) XM_009294324.2 , XM_009294324.3
    Accession Version XM_009294324.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein spire homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGA TATCCCAGAT GGACCTCGCA AGTTGGCCAT CAGCACACTA
    TCTCTGGCTA ACGGCACGTC ACCGGCTCGA AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT
    TCACTGTCCC AGAGGAAAAG GTTACTCAAA GCACCTACAC TAGCCGAACT GGACAGCTCC
    GACTCTGAGG AGGAGCAAAG CACCCGCAAA TCTGACAGCA GCTCCAGCAT ATCCACCTCA
    CTGGTTGAGG ACACGTCTCC AGAGTCTGTC ATGGGCAAAA AACCTCCACC TCAGTTCCTG
    CCCATTTCTT CCACACCTCA GCCTGATAAA CGCATCGCTC CTCAGAGGAG ACACTCCATT
    GAGAAAGAGG CTCCCACCAA CATACGCCAC TTCCTGCCTC CATCTCGACA GAACTCCAAA
    TCTCTGGAGG AGTTCTGCTT CCCTGTCGAG TGTTTGACTC TGACCGTGGA GGAGGTGATG
    CACATCAGAC AGGTTCTTGT CAAAGCCGAA CTGGAGAAGT TCCAGCAGTA TAAAGACATT
    TATAATGCAC TGAAGAAAGG AAAGCTTTGC TTCTCATGTC GGTCGAAGAA GTTCTCATTA
    TTTACCTGGT CCTACACCTG TCAGTTCTGT AAGAGGCCGG TTTGCTCACA GTGCTGCAAG
    AAGATGAAAT TGCCCTCCAA GCCTCACGCC AGCCTGCCCA TCTCCTCTTT GGGTCCCAGT
    ATCCTGCCCA AAAAAGAGCC CGGAGCATCA TCAGCCCCGA CAGATAAAAC CTCCTCCACA
    TCCAGCCACA AGAAAAACAG CCTTCAGCGC TCATTGTCTA GATCATCAAA ACACGGCGAT
    CGCTCGTCTT CTAAAGACGA GCTGGAGCTT CCGGAGCAGT TCACCGAGGA CTGGAGCACC
    ATGGAGGTCT GCGTGGACTG CAAAAAATTC ATCAACGACA TCATCAGCAA CAGCCGCCGC
    AACCTGTCCA CCAAACGTGC CCGCCTGCAC CGCCGGACAC ACTCCGTCTA CTCGTCCAGC
    ACCAGCTCCT CCAACTACAA ACCAACCGAG CGCACCATCA AAGAGGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292599.1
    CDS181..2391
    Translation

    Target ORF information:

    RefSeq Version XM_009294324.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spire-type actin nucleation factor 1a (spire1a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294324.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACTGACG GAGGAATGTT AATCTCACCG TCGGCTCTCC AGGATCCCGG AGATGGAGCG 
    CGACCTGAAG ACATCGCTAT GGATTGTACG GACGGGGAAG AGGAATTGTG CTTGGAGGAG
    ATTTTAACAC TGTACAGTCA ACCGATCAAC GAGGAACAAG CCTGGGCCGT GTGTTATCAG
    TGCTGCAGGT GGTTAACGCA AAAACACAGG CGGAAAGAGA CAGGCGTGAG TCCACCTGGA
    CGGATTGCGG GTCCTGGAGA TGTGAGGATC CGGAAGGACG GGAATGTGAA ATTATATCAG
    CCGAGCAATC CAGATAAGCA CACACCGCCC AGCTCATCAA TAGAGATCAT CGAGTCTCTG
    GGCATCATGA TCTATAAGGC TCTGGATTAT GGCCTGAAGG AGCACGAGGA AAGAGAACTG
    AGTCCTCCTC TGGAGCAACT TATCGACCTC ATGACCAACA TGGCGGACAC AGAAACAGAC
    TGTCCTGATG AGGGATACGA GGCCACGGAG GAGGAGGATG AGGGAGAAGA AGAGAACGCT
    GAAGTTTCCA ATGTTCGAGG ATACCGAGAC ATAATTTCTC TCTGTTTGTC TCACCTTCCC
    AGTCCATCTG ATGCTCCAAA CCACTACCAG GCCGTGTGTC GAGCCCTGTA CGCTGAGACC
    AAGGAGCTCC GCACTTTCCT AGAGAAGATC AAGAGTGCCA AAGAGAATTT GCGGAAGATG
    GAAGGAGAAA CAGAGGAGCC CGTCAGAGAC CTGAATGAGC TGCAGAATGC TGACTGGGCT
    CGGTTTTGGG TGCAGGTGAT GAGGGACCTG AGGCACGGCG TGAAGCTGAA GAAGGTGCAG
    GAGCGTCAGT ATAACCCTCT AGCCATCGAG TACCAGCTCA CGCCTTACGA GATGCTCATG
    GATGACATCC GATCCAAACG CTACAAACTC CGCAAGGTCA TGGTTAATGG TGACATTCCT
    CCGAGGTTAA AGAAAAGTGC TCACGAGATC ATCCTGGAGT TCATTCGATC GAGGCCTCCA
    TTAAATCCTG TTTCTGCTCG TAAACTGAAG CCTCATGCTC CTCAACCTCC GACTCTCCAT
    GAGCGTATCC TTGAGGAGAT CCGATCAGAG AGAAAGCTTA GGCCCGTGTC TCCTGATATG
    ATCCGCAGGA GTCGACTCGA TATCCCAGAT GGACCTCGCA AGTTGGCCAT CAGCACACTA
    TCTCTGGCTA ACGGCACGTC ACCGGCTCGA AGTCCTGTGA ACGGTGTAGC CGGAGGGCAT
    TCACTGTCCC AGAGGAAAAG GTTACTCAAA GCACCTACAC TAGCCGAACT GGACAGCTCC
    GACTCTGAGG AGGAGCAAAG CACCCGCAAA TCTGACAGCA GCTCCAGCAT ATCCACCTCA
    CTGGTTGAGG ACACGTCTCC AGAGTCTGTC ATGGGCAAAA AACCTCCACC TCAGTTCCTG
    CCCATTTCTT CCACACCTCA GCCTGATAAA CGCATCGCTC CTCAGAGGAG ACACTCCATT
    GAGAAAGAGG CTCCCACCAA CATACGCCAC TTCCTGCCTC CATCTCGACA GAACTCCAAA
    TCTCTGGAGG AGTTCTGCTT CCCTGTCGAG TGTTTGACTC TGACCGTGGA GGAGGTGATG
    CACATCAGAC AGGTTCTTGT CAAAGCCGAA CTGGAGAAGT TCCAGCAGTA TAAAGACATT
    TATAATGCAC TGAAGAAAGG AAAGCTTTGC TTCTCATGTC GGTCGAAGAA GTTCTCATTA
    TTTACCTGGT CCTACACCTG TCAGTTCTGT AAGAGGCCGG TTTGCTCACA GTGCTGCAAG
    AAGATGAAAT TGCCCTCCAA GCCTCACGCC AGCCTGCCCA TCTCCTCTTT GGGTCCCAGT
    ATCCTGCCCA AAAAAGAGCC CGGAGCATCA TCAGCCCCGA CAGATAAAAC CTCCTCCACA
    TCCAGCCACA AGAAAAACAG CCTTCAGCGC TCATTGTCTA GATCATCAAA ACACGGCGAT
    CGCTCGTCTT CTAAAGACGA GCTGGAGCTT CCGGAGCAGT TCACCGAGGA CTGGAGCACC
    ATGGAGGTCT GCGTGGACTG CAAAAAATTC ATCAACGACA TCATCAGCAA CAGCCGCCGC
    AACCTGTCCA CCAAACGTGC CCGCCTGCAC CGCCGGACAC ACTCCGTCTA CTCGTCCAGC
    ACCAGCTCCT CCAACTACAA ACCAACCGAG CGCACCATCA AAGAGGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.