mettl13 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mettl13
Entrez Gene ID	557908
Full Name	methyltransferase like 13
Synonyms	si:dkey-19f21.2,zgc:152769
General protein information	Preferred Names methyltransferase like 13 Names methyltransferase-like protein 13
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044769.4	NP_001038234.3	eEF1A lysine and N-terminal methyltransferase

Pathways from BioSystems

Homologies

Homo sapiens (human)	METTL13	NP_057019.3
Mus musculus (house mouse)	Mettl13	NP_659126.1
Danio rerio (zebrafish)	mettl13	NP_001038234.2
Arabidopsis thaliana (thale cress)	AT2G31740	NP_850171.1
Pan troglodytes (chimpanzee)	METTL13	XP_001146989.1
Bos taurus (cattle)	METTL13	NP_001092555.1
Gallus gallus (chicken)	METTL13	XP_004943249.1
Drosophila melanogaster (fruit fly)	CG2614	NP_610045.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008472	XP_316976.2
Caenorhabditis elegans (roundworm)	CELE_C01B10.8	NP_501024.1
Oryza sativa (rice)	Os04g0379300	NP_001052588.1
	METTL13	XP_001100091.1
Canis lupus familiaris (dog)	METTL13	XP_850646.1
Rattus norvegicus (Norway rat)	Mettl13	NP_001127973.1
Xenopus tropicalis (tropical clawed frog)	mettl13	NP_001107964.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Biswas G, Kinoshita S, Kono T, Hikima J, Sakai M
Marine genomics2225-36(2015 Aug)

Evolution and genomic organization of muscle microRNAs in fish genomes.
Nachtigall PG, Dias MC, Pinhal D
BMC evolutionary biology14196(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mettl13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mettl13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00286	NM_001044769.4 Latest version!	Danio rerio methyltransferase like 13 (mettl13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mettl13 ( NM_001044769.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038234.3 Danio rerio methyltransferase like 13 (mettl13), mRNA.

Price & Availability↑

CloneID	ODa00286
Clone ID Related Accession (Same CDS sequence)	NM_001044769.4
Accession Version	NM_001044769.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Danio rerio(zebrafish)
Product	eEF1A lysine and N-terminal methyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX470128.8. On Dec 7, 2013 this sequence version replaced NM_001044769.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01016210.1, GFIL01026251.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTTGT TACCTCGCAC CGCTGAGGAG TTCAGCTCAG CGGATTACTG GGAGCGCTTT 
TTCCGTAAGA GAGGAGAGAA GGCGTTTGAG TGGTACGGAG ACTACAACTC ACTCTGCGGG 
GTTCTGCATA AATACATCAA ACCACGAGAT AAGGTATTGG TGGTGGGCTG TGGTAACTCT 
GAACTCAGTG AACAGCTCTA TGATGTTGGC TACCGTCAGC TGACCAACAT TGACATCAGC 
GAGACGGTGG TCTCCCATAT GAACCAGAGG AATGCAGAGC GCCGTCCAGA CCTCTCCTTC 
CAGCAGCTGG ATGCCACCCA GACCGGCTTC GAGAGCGGGA GCTTTCAGGT GACTCTGGAT 
AAAGGCACTC TGGACGCCAT GGCTTCAGAA GAGGATGGTG CTCTTGCAGG CCGGATGCTG 
GCAGAGGTTG GCAGAGTTTT GGCTGTGGGC GGGAGGTATG TCTGCATAAC GTTAGCCCAG 
GAGCATGTCA TAAAGTTAGC AGTGGAGCAT TTTGTTAAGG GATGGGCAGT GAGAGTACAT 
TGTCTAACCG GACAACAGAA CGAAGAGTCC GATTCCTCCT TTGCACTCCC CGTTTTTGTG 
TTGGTTTGCA CTAAGTTTCG ACAAGCCCCT CCATTTGCTG TTCTTGAGCT GTGCCAGGGG 
GAGGATGGCG CTCCGGCTAG ACTGGCATCA GTAGAGGAGC TGCTGTCTGC AGTCAAGGAG 
AGGCAGGCTT ATAATCTAAT GCTGCATAAA CTCAGAGGAG GAACAGACTC AAGCAGCACA 
CCCTCACTCA CGCTGTGCCA CGCAGCCAGC GGACGCCCAC GGTACACTCT GACCATACAG 
GACGGCCCAC CTTCTGCTAA GACCCCGCGG AGCAATCACT TCGCCATCTT TATTGTTCCT 
CAGGGTCGTG AGTCTGATTG GTTATATGGT TCAGCTGAAG GTCGAGCTCA GTTAGCGTCC 
AGTGCAAAAT TCAGACGTCT TGTCATTGTG GCAATGCATC GAGACCAAGA ATATGAAGAC 
ATGCAGGCTG TCCAATCAGA GCTCTCGCCA GTGGTCATGG AACTCGCCCC TCCTGGAATG 
CCAGCCAATC AGCAGGTGCC GTTTCTGTCA GTGGGAGGAG ATCTAGGCTG GAGGGAAGTG 
ATTGGACGAG GGCTCAGTGC TTTGACTGGA GAGTATTCAG TGGAGGATGT GAGAGGAGAG 
GACGGTTACC TCTATCGCAG GCTTATCTTT ATGAATAATT CTCAGCTGGT GCAGTCAGAG 
AGCAGACTCC AATCTGCTGC TGCAGCTTCT TCTGCTTCTA AAAAGAAGAA CAAGAAAAAA 
GCCAAACAGC CTGCTTCAAC TGGTGCTAAA GATCGCAGTG TGGACAGAGG GTTTCTCTGC 
TGTACGCATC ATGAGGTCAT GGTGGCGGGT TTAGCAATGC TAGGAATGGA TGCAATCAAT 
AATAAAGATC AGCCAGTGTC TGTGTTGCTG GTGGGTCTAG GTGGTGGCGG CCTGCCTCAG 
TTTGTGCGGG ACTTTGTGCC ATGTGCTCGG GTGGAGGTGG TTGAACTGGA TCCGGTGGTG 
CTGGATGTCG CTCAGACCTG GTTTGGATTT CAGATTGACG ACAGGCTAAA AGTTACACTG 
GGAGACGGAC TGGAGCACAT CACAACCCTG GAGAGTGAAG GGGAGCGCTA TTTTGATGTC 
ATCATGTTTG ACGTTGACAG CAAAGACACC ACCCTGGGCA TGAGCTGCCC TCCACCTGCC 
TTTGTAGAAA CATCTCTTCT GAAGAAAGTT TACAGCTTGT TAAGCCCCAG AGGTCTGTTC 
ATGTTGAACC TGGTGTGTCG CGACTCAGCT CTTAGGAAAT CAGTTCTTGA CCGTGTTCAT 
TCTGTGTTCC CATGTGTGTT TTCTCGTGGA ATCGAGGGTG AAGTCAATGA GGTGCTGCTG 
TGTTGCAGAA GCTCAGGAGA ACACAAACCT CACACCGTCC CACAGACATT ACAGCAAACA 
GCAAAAGACC TGCAGAAGAC ACTACGAGCA AACAGCCAGA CTGCACCTCA GATAGACATT 
ACTGCAATGC TGAATGACCT GAAAGTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038234.3
CDS	118..2190
Translation	MSLLPRTAEEFSSADYWERFFRKRGEKAFEWYGDYNSLCGVLHKYIKPRDKVLVVGCGNSELSEQLYDVGYRQLTNIDISETVVSHMNQRNAERRPDLSFQQLDATQTGFESGSFQVTLDKGTLDAMASEEDGALAGRMLAEVGRVLAVGGRYVCITLAQEHVIKLAVEHFVKGWAVRVHCLTGQQNEESDSSFALPVFVLVCTKFRQAPPFAVLELCQGEDGAPARLASVEELLSAVKERQAYNLMLHKLRGGTDSSSTPSLTLCHAASGRPRYTLTIQDGPPSAKTPRSNHFAIFIVPQGRESDWLYGSAEGRAQLASSAKFRRLVIVAMHRDQEYEDMQAVQSELSPVVMELAPPGMPANQQVPFLSVGGDLGWREVIGRGLSALTGEYSVEDVRGEDGYLYRRLIFMNNSQLVQSESRLQSAAAASSASKKKNKKKAKQPASTGAKDRSVDRGFLCCTHHEVMVAGLAMLGMDAINNKDQPVSVLLVGLGGGGLPQFVRDFVPCARVEVVELDPVVLDVAQTWFGFQIDDRLKVTLGDGLEHITTLESEGERYFDVIMFDVDSKDTTLGMSCPPPAFVETSLLKKVYSLLSPRGLFMLNLVCRDSALRKSVLDRVHSVFPCVFSRGIEGEVNEVLLCCRSSGEHKPHTVPQTLQQTAKDLQKTLRANSQTAPQIDITAMLNDLKVV

Target ORF information:

RefSeq Version	NM_001044769.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyltransferase like 13 (mettl13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044769.4

ORF Insert Sequence:

ATGAGTTTGT TACCTCGCAC CGCTGAGGAG TTCAGCTCAG CGGATTACTG GGAGCGCTTT 
TTCCGTAAGA GAGGAGAGAA GGCGTTTGAG TGGTACGGAG ACTACAACTC ACTCTGCGGG 
GTTCTGCATA AATACATCAA ACCACGAGAT AAGGTATTGG TGGTGGGCTG TGGTAACTCT 
GAACTCAGTG AACAGCTCTA TGATGTTGGC TACCGTCAGC TGACCAACAT TGACATCAGC 
GAGACGGTGG TCTCCCATAT GAACCAGAGG AATGCAGAGC GCCGTCCAGA CCTCTCCTTC 
CAGCAGCTGG ATGCCACCCA GACCGGCTTC GAGAGCGGGA GCTTTCAGGT GACTCTGGAT 
AAAGGCACTC TGGACGCCAT GGCTTCAGAA GAGGATGGTG CTCTTGCAGG CCGGATGCTG 
GCAGAGGTTG GCAGAGTTTT GGCTGTGGGC GGGAGGTATG TCTGCATAAC GTTAGCCCAG 
GAGCATGTCA TAAAGTTAGC AGTGGAGCAT TTTGTTAAGG GATGGGCAGT GAGAGTACAT 
TGTCTAACCG GACAACAGAA CGAAGAGTCC GATTCCTCCT TTGCACTCCC CGTTTTTGTG 
TTGGTTTGCA CTAAGTTTCG ACAAGCCCCT CCATTTGCTG TTCTTGAGCT GTGCCAGGGG 
GAGGATGGCG CTCCGGCTAG ACTGGCATCA GTAGAGGAGC TGCTGTCTGC AGTCAAGGAG 
AGGCAGGCTT ATAATCTAAT GCTGCATAAA CTCAGAGGAG GAACAGACTC AAGCAGCACA 
CCCTCACTCA CGCTGTGCCA CGCAGCCAGC GGACGCCCAC GGTACACTCT GACCATACAG 
GACGGCCCAC CTTCTGCTAA GACCCCGCGG AGCAATCACT TCGCCATCTT TATTGTTCCT 
CAGGGTCGTG AGTCTGATTG GTTATATGGT TCAGCTGAAG GTCGAGCTCA GTTAGCGTCC 
AGTGCAAAAT TCAGACGTCT TGTCATTGTG GCAATGCATC GAGACCAAGA ATATGAAGAC 
ATGCAGGCTG TCCAATCAGA GCTCTCGCCA GTGGTCATGG AACTCGCCCC TCCTGGAATG 
CCAGCCAATC AGCAGGTGCC GTTTCTGTCA GTGGGAGGAG ATCTAGGCTG GAGGGAAGTG 
ATTGGACGAG GGCTCAGTGC TTTGACTGGA GAGTATTCAG TGGAGGATGT GAGAGGAGAG 
GACGGTTACC TCTATCGCAG GCTTATCTTT ATGAATAATT CTCAGCTGGT GCAGTCAGAG 
AGCAGACTCC AATCTGCTGC TGCAGCTTCT TCTGCTTCTA AAAAGAAGAA CAAGAAAAAA 
GCCAAACAGC CTGCTTCAAC TGGTGCTAAA GATCGCAGTG TGGACAGAGG GTTTCTCTGC 
TGTACGCATC ATGAGGTCAT GGTGGCGGGT TTAGCAATGC TAGGAATGGA TGCAATCAAT 
AATAAAGATC AGCCAGTGTC TGTGTTGCTG GTGGGTCTAG GTGGTGGCGG CCTGCCTCAG 
TTTGTGCGGG ACTTTGTGCC ATGTGCTCGG GTGGAGGTGG TTGAACTGGA TCCGGTGGTG 
CTGGATGTCG CTCAGACCTG GTTTGGATTT CAGATTGACG ACAGGCTAAA AGTTACACTG 
GGAGACGGAC TGGAGCACAT CACAACCCTG GAGAGTGAAG GGGAGCGCTA TTTTGATGTC 
ATCATGTTTG ACGTTGACAG CAAAGACACC ACCCTGGGCA TGAGCTGCCC TCCACCTGCC 
TTTGTAGAAA CATCTCTTCT GAAGAAAGTT TACAGCTTGT TAAGCCCCAG AGGTCTGTTC 
ATGTTGAACC TGGTGTGTCG CGACTCAGCT CTTAGGAAAT CAGTTCTTGA CCGTGTTCAT 
TCTGTGTTCC CATGTGTGTT TTCTCGTGGA ATCGAGGGTG AAGTCAATGA GGTGCTGCTG 
TGTTGCAGAA GCTCAGGAGA ACACAAACCT CACACCGTCC CACAGACATT ACAGCAAACA 
GCAAAAGACC TGCAGAAGAC ACTACGAGCA AACAGCCAGA CTGCACCTCA GATAGACATT 
ACTGCAATGC TGAATGACCT GAAAGTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Marine genomics2225-36(2015 Aug)
Biswas G,Kinoshita S,Kono T,Hikima J,Sakai M

Evolution and genomic organization of muscle microRNAs in fish genomes.
BMC evolutionary biology14196(2014 Sep)
Nachtigall PG,Dias MC,Pinhal D

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mettl13 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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