NRD1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRD1B
Entrez Gene ID	557565
Full Name	nardilysin b (N-arginine dibasic convertase)
Synonyms	nrd1,si:dkey-171o17.4
General protein information	Preferred Names nardilysin b (N-arginine dibasic convertase) Names nardilysin nardilysin (N-arginine dibasic convertase)
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296247.2	XP_009294522.1	nardilysin isoform X1
XM_009296248.2	XP_009294523.1	nardilysin isoform X1
XM_009296249.3	XP_009294524.1	nardilysin isoform X2
XM_009296248.1	XP_009294523.1	nardilysin isoform X1
XM_009296247.1	XP_009294522.1	nardilysin isoform X1
NM_001044715.2	NP_001038180.2	nardilysin
XM_009296249.2	XP_009294524.1	nardilysin isoform X2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG2025	NP_572757.2
Danio rerio (zebrafish)	nrd1	NP_001038180.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010315	XP_320119.6
Drosophila melanogaster (fruit fly)	CG10588	NP_649271.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NRD1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRD1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42630	XM_009296249.3 Latest version!	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa02234	NM_001044715.2 Latest version!	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42629	XM_009296247.2 Latest version!	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42629	XM_009296248.2 Latest version!	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa42629	XM_009296248.1	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42629	XM_009296247.1	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42630	XM_009296249.2	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRD1B ( XM_009296249.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294524.1 Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42630
Clone ID Related Accession (Same CDS sequence)	XM_009296249.3 , XM_009296249.2
Accession Version	XM_009296249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nardilysin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCTG ATGCTGTAGA CAGAGAGGTG GAGGCTGTAG ACAGCGAGTA CCAGATGGCC 
CAGCCTTTAG ACTCAAACCG TAAAGAGATG CTGTTTGGAA GTCTAGCAAA AGCCGGTCAT 
CCGATGAGCA AGTTCTTCTG GGGAAATGCT CAAACACTAA AACAAGAGCC GAGAGAGAAG 
AAAATCAACA CTTATGAGCG TCTGAGAGAC TTCTGGAGGA GATATTACTC TGCACAATAC 
ATGACACTTG CTGTCCAATC CAAAGAGACA CTTGATACGC TGGAGGAATG GGTGCGAGAG 
ATCTTCGTTC AGATTCCTAA CAATGGTCTG CCAAAGGCTG ATTTCTCAGA CCTTCAGGAC 
CCGTTCGACA CCCCAGATTT TTGCAAACTG TACAGAGTGG TGCCTGTGCA AAAGGTTCAC 
GCTCTGACCA TCAGCTGGGC TCTTCCACCT CAGGCCAAAC ATTACAGAGT GAAACCCTTG 
CATTATATTT CCTGGCTGAT CGGTCATGAA GGAGTGGGAA GTGTCCTGTC TTTACTCAGA 
AAGAGGTGTT GGGCTCTGAG TCTGTTTGGA GGAAACAGTG AGTCGGGCTT TGATCAAAAC 
TCCACGTACT CCATCTTTAG CATTTCTATC ACTTTGAGCG ACGAAGGTCT TCAGAACTTC 
CTCCAGGTCA TTCATATCAT CTTCCAGTAT CTGAAGATGC TTCAGTCTGT TGGACCTCAG 
CAGAGGATCT ACGAGGAGAT CCAGAAGATT GAAGCCAATG AGTTTCACTA TCAAGAGCAG 
ACAGAGCCAA TTGAGTTTGT GGCGAACATG AGTGAGAACA TGCAGCTGTT CCCGAAAGAG 
CACTTCCTGT GTGGAGACCA GCTGATGTTT GACTTCAACC CAGAGGTCAT TTCAGCTGCT 
TTGTCACTCT TGACTCCAGG AAAGGCTAAT ATTTTACTGC TGTCACCTCA GCATGATGGT 
CTCTGCCCAC TCAAGGAGAA GTGGTTCGGG ACACAGTATA GTGTGGAAGA TATTCCTCAG 
GAATTCCGTG ATCTTTGGGC TGGAGATTTT CCTCTGCATC CTGAGCTTCA GCTCCCAGCT 
GAGAACAAGT TTATAGCCAC GGATTTCACC CTCAGAACAT CAGATTGTCC TGATACAGAC 
TTCCCAGTCA AAATAATAGA CAACGAGAGA GGCCGTCTGT GGTTTAGAAA AGACAACAAG 
TTCAAGATAC CCAAAGCTTA TGCCCGCTTC CAGCTGCTCA CTCCATTCAT ACAGGAATCA 
CCTAAAAATT TGGTCCTGTT TGATCTCTTC GTGAATATTG TGGCTCATAA TCTGGCAGAG 
CTGGCATATG ACGCTGAAGT GGCTCAACTA CAGTATAATC TACTGCCAGG AGATCACGGC 
CTGTTCATCA GACTCAAAGG ATTCAACCAC AAACTGCCTT TGCTCCTGAA GCTGATTGTA 
GATCACCTGG CCGACTTCAG CGCCACTCCA GACGTGTTTA ACATGTTCAT TGAGCAGCTG 
AAGAAAACCT ACTACATCAT CCTCATTAGA CCAGAACGCC TTGGAAAAGA TGTTCGGCTG 
CAGATTTTGG AGCACCATCG CTGGTCTGTG ATGCAGAAAT ATGAAGCCAT CATGGCGGAT 
CCGAGTGTGG CTGATCTCAT GACCTTTGCC AATCGCTTTA AGGCTGAGTT ATTTGTGGAA 
GGACTCGTAC AAGGGAACTT CACAAGTGCT GAGTCCAAAG AGTTTCTGCA GTGTTTTATT 
GAGAAACTCA AGTATGCACC TCATCCCATT GAACCTCCTG TGCTGTTTCG AGTGGTGGAG 
TTACCACAAA CACACCACTT ATGTAAAGTC CAGTCGCTCA ATAAAGCAGA CGCAAACTCT 
GAAGTCACCG TATACTATCA GACTGGGCTG AAGAACTTGC GAGAGCATAC ACTGATGGAG 
CTGCTTGTTA TGCACATGGA AGAGCCCTGC TTTGATTTCC TGAGGACAAA GGAAACTCTA 
GGGTATCAGG TCTATCCCAT ATGCAGAAAC ACATCCGGCA TTCTTGGCTT CTCCGTTACT 
GTCGAAACTC AGGCCACCAA ATTCAGCACG GACTTTGTGG AAGGTAAAAT CGAAGCATTT 
CTGGTCAGCT TTGGAGAGAA GTTGGTTCAG CTGTCAGACG AGGCGTTTGG AGCTCAAGTG 
ACAGCGCTGA TCAAGCTGAA GGAGTGTGAA GACACACAGC TCGGGGACGA AGTGGACAGA 
AACTGGTTCG AAGTGGTCAC TCAGCAGTAT GTCTTTGACA GGCTCAATAA AGAAATTGAA 
ATCCTGAAAG ACGTCACCAA AGATGAACTC GTATCATTTT ACATGGAGCA CCGAAAAGAA 
AACAGTAGAA AACTAAGCAT ACATGTCGTT GGATTCGGAG AAGAAGAGAA GGAAATCCAT 
GGAGAAGACC AGTGCAGTTC AAACCCAGAG ACTTCTTCAT ATGCAAATAT TTCCAAACTC 
ACTTTCCTCT CAGCGGCGGC CATGAAGGAG AAGTCCACCA TGATAACAGA CATCCGCGCT 
TTCACATCTT CACTCCATCT GCACCCCTAC CACAAAATCC TGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294524.1
CDS	87..2654
Translation	MIPDAVDREVEAVDSEYQMAQPLDSNRKEMLFGSLAKAGHPMSKFFWGNAQTLKQEPREKKINTYERLRDFWRRYYSAQYMTLAVQSKETLDTLEEWVREIFVQIPNNGLPKADFSDLQDPFDTPDFCKLYRVVPVQKVHALTISWALPPQAKHYRVKPLHYISWLIGHEGVGSVLSLLRKRCWALSLFGGNSESGFDQNSTYSIFSISITLSDEGLQNFLQVIHIIFQYLKMLQSVGPQQRIYEEIQKIEANEFHYQEQTEPIEFVANMSENMQLFPKEHFLCGDQLMFDFNPEVISAALSLLTPGKANILLLSPQHDGLCPLKEKWFGTQYSVEDIPQEFRDLWAGDFPLHPELQLPAENKFIATDFTLRTSDCPDTDFPVKIIDNERGRLWFRKDNKFKIPKAYARFQLLTPFIQESPKNLVLFDLFVNIVAHNLAELAYDAEVAQLQYNLLPGDHGLFIRLKGFNHKLPLLLKLIVDHLADFSATPDVFNMFIEQLKKTYYIILIRPERLGKDVRLQILEHHRWSVMQKYEAIMADPSVADLMTFANRFKAELFVEGLVQGNFTSAESKEFLQCFIEKLKYAPHPIEPPVLFRVVELPQTHHLCKVQSLNKADANSEVTVYYQTGLKNLREHTLMELLVMHMEEPCFDFLRTKETLGYQVYPICRNTSGILGFSVTVETQATKFSTDFVEGKIEAFLVSFGEKLVQLSDEAFGAQVTALIKLKECEDTQLGDEVDRNWFEVVTQQYVFDRLNKEIEILKDVTKDELVSFYMEHRKENSRKLSIHVVGFGEEEKEIHGEDQCSSNPETSSYANISKLTFLSAAAMKEKSTMITDIRAFTSSLHLHPYHKILI

Target ORF information:

RefSeq Version	XM_009296249.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296249.3

ORF Insert Sequence:

ATGATTCCTG ATGCTGTAGA CAGAGAGGTG GAGGCTGTAG ACAGCGAGTA CCAGATGGCC 
CAGCCTTTAG ACTCAAACCG TAAAGAGATG CTGTTTGGAA GTCTAGCAAA AGCCGGTCAT 
CCGATGAGCA AGTTCTTCTG GGGAAATGCT CAAACACTAA AACAAGAGCC GAGAGAGAAG 
AAAATCAACA CTTATGAGCG TCTGAGAGAC TTCTGGAGGA GATATTACTC TGCACAATAC 
ATGACACTTG CTGTCCAATC CAAAGAGACA CTTGATACGC TGGAGGAATG GGTGCGAGAG 
ATCTTCGTTC AGATTCCTAA CAATGGTCTG CCAAAGGCTG ATTTCTCAGA CCTTCAGGAC 
CCGTTCGACA CCCCAGATTT TTGCAAACTG TACAGAGTGG TGCCTGTGCA AAAGGTTCAC 
GCTCTGACCA TCAGCTGGGC TCTTCCACCT CAGGCCAAAC ATTACAGAGT GAAACCCTTG 
CATTATATTT CCTGGCTGAT CGGTCATGAA GGAGTGGGAA GTGTCCTGTC TTTACTCAGA 
AAGAGGTGTT GGGCTCTGAG TCTGTTTGGA GGAAACAGTG AGTCGGGCTT TGATCAAAAC 
TCCACGTACT CCATCTTTAG CATTTCTATC ACTTTGAGCG ACGAAGGTCT TCAGAACTTC 
CTCCAGGTCA TTCATATCAT CTTCCAGTAT CTGAAGATGC TTCAGTCTGT TGGACCTCAG 
CAGAGGATCT ACGAGGAGAT CCAGAAGATT GAAGCCAATG AGTTTCACTA TCAAGAGCAG 
ACAGAGCCAA TTGAGTTTGT GGCGAACATG AGTGAGAACA TGCAGCTGTT CCCGAAAGAG 
CACTTCCTGT GTGGAGACCA GCTGATGTTT GACTTCAACC CAGAGGTCAT TTCAGCTGCT 
TTGTCACTCT TGACTCCAGG AAAGGCTAAT ATTTTACTGC TGTCACCTCA GCATGATGGT 
CTCTGCCCAC TCAAGGAGAA GTGGTTCGGG ACACAGTATA GTGTGGAAGA TATTCCTCAG 
GAATTCCGTG ATCTTTGGGC TGGAGATTTT CCTCTGCATC CTGAGCTTCA GCTCCCAGCT 
GAGAACAAGT TTATAGCCAC GGATTTCACC CTCAGAACAT CAGATTGTCC TGATACAGAC 
TTCCCAGTCA AAATAATAGA CAACGAGAGA GGCCGTCTGT GGTTTAGAAA AGACAACAAG 
TTCAAGATAC CCAAAGCTTA TGCCCGCTTC CAGCTGCTCA CTCCATTCAT ACAGGAATCA 
CCTAAAAATT TGGTCCTGTT TGATCTCTTC GTGAATATTG TGGCTCATAA TCTGGCAGAG 
CTGGCATATG ACGCTGAAGT GGCTCAACTA CAGTATAATC TACTGCCAGG AGATCACGGC 
CTGTTCATCA GACTCAAAGG ATTCAACCAC AAACTGCCTT TGCTCCTGAA GCTGATTGTA 
GATCACCTGG CCGACTTCAG CGCCACTCCA GACGTGTTTA ACATGTTCAT TGAGCAGCTG 
AAGAAAACCT ACTACATCAT CCTCATTAGA CCAGAACGCC TTGGAAAAGA TGTTCGGCTG 
CAGATTTTGG AGCACCATCG CTGGTCTGTG ATGCAGAAAT ATGAAGCCAT CATGGCGGAT 
CCGAGTGTGG CTGATCTCAT GACCTTTGCC AATCGCTTTA AGGCTGAGTT ATTTGTGGAA 
GGACTCGTAC AAGGGAACTT CACAAGTGCT GAGTCCAAAG AGTTTCTGCA GTGTTTTATT 
GAGAAACTCA AGTATGCACC TCATCCCATT GAACCTCCTG TGCTGTTTCG AGTGGTGGAG 
TTACCACAAA CACACCACTT ATGTAAAGTC CAGTCGCTCA ATAAAGCAGA CGCAAACTCT 
GAAGTCACCG TATACTATCA GACTGGGCTG AAGAACTTGC GAGAGCATAC ACTGATGGAG 
CTGCTTGTTA TGCACATGGA AGAGCCCTGC TTTGATTTCC TGAGGACAAA GGAAACTCTA 
GGGTATCAGG TCTATCCCAT ATGCAGAAAC ACATCCGGCA TTCTTGGCTT CTCCGTTACT 
GTCGAAACTC AGGCCACCAA ATTCAGCACG GACTTTGTGG AAGGTAAAAT CGAAGCATTT 
CTGGTCAGCT TTGGAGAGAA GTTGGTTCAG CTGTCAGACG AGGCGTTTGG AGCTCAAGTG 
ACAGCGCTGA TCAAGCTGAA GGAGTGTGAA GACACACAGC TCGGGGACGA AGTGGACAGA 
AACTGGTTCG AAGTGGTCAC TCAGCAGTAT GTCTTTGACA GGCTCAATAA AGAAATTGAA 
ATCCTGAAAG ACGTCACCAA AGATGAACTC GTATCATTTT ACATGGAGCA CCGAAAAGAA 
AACAGTAGAA AACTAAGCAT ACATGTCGTT GGATTCGGAG AAGAAGAGAA GGAAATCCAT 
GGAGAAGACC AGTGCAGTTC AAACCCAGAG ACTTCTTCAT ATGCAAATAT TTCCAAACTC 
ACTTTCCTCT CAGCGGCGGC CATGAAGGAG AAGTCCACCA TGATAACAGA CATCCGCGCT 
TTCACATCTT CACTCCATCT GCACCCCTAC CACAAAATCC TGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRD1B ( XM_009296249.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294524.1 Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42630
Clone ID Related Accession (Same CDS sequence)	XM_009296249.3 , XM_009296249.2
Accession Version	XM_009296249.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nardilysin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCTG ATGCTGTAGA CAGAGAGGTG GAGGCTGTAG ACAGCGAGTA CCAGATGGCC 
CAGCCTTTAG ACTCAAACCG TAAAGAGATG CTGTTTGGAA GTCTAGCAAA AGCCGGTCAT 
CCGATGAGCA AGTTCTTCTG GGGAAATGCT CAAACACTAA AACAAGAGCC GAGAGAGAAG 
AAAATCAACA CTTATGAGCG TCTGAGAGAC TTCTGGAGGA GATATTACTC TGCACAATAC 
ATGACACTTG CTGTCCAATC CAAAGAGACA CTTGATACGC TGGAGGAATG GGTGCGAGAG 
ATCTTCGTTC AGATTCCTAA CAATGGTCTG CCAAAGGCTG ATTTCTCAGA CCTTCAGGAC 
CCGTTCGACA CCCCAGATTT TTGCAAACTG TACAGAGTGG TGCCTGTGCA AAAGGTTCAC 
GCTCTGACCA TCAGCTGGGC TCTTCCACCT CAGGCCAAAC ATTACAGAGT GAAACCCTTG 
CATTATATTT CCTGGCTGAT CGGTCATGAA GGAGTGGGAA GTGTCCTGTC TTTACTCAGA 
AAGAGGTGTT GGGCTCTGAG TCTGTTTGGA GGAAACAGTG AGTCGGGCTT TGATCAAAAC 
TCCACGTACT CCATCTTTAG CATTTCTATC ACTTTGAGCG ACGAAGGTCT TCAGAACTTC 
CTCCAGGTCA TTCATATCAT CTTCCAGTAT CTGAAGATGC TTCAGTCTGT TGGACCTCAG 
CAGAGGATCT ACGAGGAGAT CCAGAAGATT GAAGCCAATG AGTTTCACTA TCAAGAGCAG 
ACAGAGCCAA TTGAGTTTGT GGCGAACATG AGTGAGAACA TGCAGCTGTT CCCGAAAGAG 
CACTTCCTGT GTGGAGACCA GCTGATGTTT GACTTCAACC CAGAGGTCAT TTCAGCTGCT 
TTGTCACTCT TGACTCCAGG AAAGGCTAAT ATTTTACTGC TGTCACCTCA GCATGATGGT 
CTCTGCCCAC TCAAGGAGAA GTGGTTCGGG ACACAGTATA GTGTGGAAGA TATTCCTCAG 
GAATTCCGTG ATCTTTGGGC TGGAGATTTT CCTCTGCATC CTGAGCTTCA GCTCCCAGCT 
GAGAACAAGT TTATAGCCAC GGATTTCACC CTCAGAACAT CAGATTGTCC TGATACAGAC 
TTCCCAGTCA AAATAATAGA CAACGAGAGA GGCCGTCTGT GGTTTAGAAA AGACAACAAG 
TTCAAGATAC CCAAAGCTTA TGCCCGCTTC CAGCTGCTCA CTCCATTCAT ACAGGAATCA 
CCTAAAAATT TGGTCCTGTT TGATCTCTTC GTGAATATTG TGGCTCATAA TCTGGCAGAG 
CTGGCATATG ACGCTGAAGT GGCTCAACTA CAGTATAATC TACTGCCAGG AGATCACGGC 
CTGTTCATCA GACTCAAAGG ATTCAACCAC AAACTGCCTT TGCTCCTGAA GCTGATTGTA 
GATCACCTGG CCGACTTCAG CGCCACTCCA GACGTGTTTA ACATGTTCAT TGAGCAGCTG 
AAGAAAACCT ACTACATCAT CCTCATTAGA CCAGAACGCC TTGGAAAAGA TGTTCGGCTG 
CAGATTTTGG AGCACCATCG CTGGTCTGTG ATGCAGAAAT ATGAAGCCAT CATGGCGGAT 
CCGAGTGTGG CTGATCTCAT GACCTTTGCC AATCGCTTTA AGGCTGAGTT ATTTGTGGAA 
GGACTCGTAC AAGGGAACTT CACAAGTGCT GAGTCCAAAG AGTTTCTGCA GTGTTTTATT 
GAGAAACTCA AGTATGCACC TCATCCCATT GAACCTCCTG TGCTGTTTCG AGTGGTGGAG 
TTACCACAAA CACACCACTT ATGTAAAGTC CAGTCGCTCA ATAAAGCAGA CGCAAACTCT 
GAAGTCACCG TATACTATCA GACTGGGCTG AAGAACTTGC GAGAGCATAC ACTGATGGAG 
CTGCTTGTTA TGCACATGGA AGAGCCCTGC TTTGATTTCC TGAGGACAAA GGAAACTCTA 
GGGTATCAGG TCTATCCCAT ATGCAGAAAC ACATCCGGCA TTCTTGGCTT CTCCGTTACT 
GTCGAAACTC AGGCCACCAA ATTCAGCACG GACTTTGTGG AAGGTAAAAT CGAAGCATTT 
CTGGTCAGCT TTGGAGAGAA GTTGGTTCAG CTGTCAGACG AGGCGTTTGG AGCTCAAGTG 
ACAGCGCTGA TCAAGCTGAA GGAGTGTGAA GACACACAGC TCGGGGACGA AGTGGACAGA 
AACTGGTTCG AAGTGGTCAC TCAGCAGTAT GTCTTTGACA GGCTCAATAA AGAAATTGAA 
ATCCTGAAAG ACGTCACCAA AGATGAACTC GTATCATTTT ACATGGAGCA CCGAAAAGAA 
AACAGTAGAA AACTAAGCAT ACATGTCGTT GGATTCGGAG AAGAAGAGAA GGAAATCCAT 
GGAGAAGACC AGTGCAGTTC AAACCCAGAG ACTTCTTCAT ATGCAAATAT TTCCAAACTC 
ACTTTCCTCT CAGCGGCGGC CATGAAGGAG AAGTCCACCA TGATAACAGA CATCCGCGCT 
TTCACATCTT CACTCCATCT GCACCCCTAC CACAAAATCC TGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294524.1
CDS	95..2662
Translation	MIPDAVDREVEAVDSEYQMAQPLDSNRKEMLFGSLAKAGHPMSKFFWGNAQTLKQEPREKKINTYERLRDFWRRYYSAQYMTLAVQSKETLDTLEEWVREIFVQIPNNGLPKADFSDLQDPFDTPDFCKLYRVVPVQKVHALTISWALPPQAKHYRVKPLHYISWLIGHEGVGSVLSLLRKRCWALSLFGGNSESGFDQNSTYSIFSISITLSDEGLQNFLQVIHIIFQYLKMLQSVGPQQRIYEEIQKIEANEFHYQEQTEPIEFVANMSENMQLFPKEHFLCGDQLMFDFNPEVISAALSLLTPGKANILLLSPQHDGLCPLKEKWFGTQYSVEDIPQEFRDLWAGDFPLHPELQLPAENKFIATDFTLRTSDCPDTDFPVKIIDNERGRLWFRKDNKFKIPKAYARFQLLTPFIQESPKNLVLFDLFVNIVAHNLAELAYDAEVAQLQYNLLPGDHGLFIRLKGFNHKLPLLLKLIVDHLADFSATPDVFNMFIEQLKKTYYIILIRPERLGKDVRLQILEHHRWSVMQKYEAIMADPSVADLMTFANRFKAELFVEGLVQGNFTSAESKEFLQCFIEKLKYAPHPIEPPVLFRVVELPQTHHLCKVQSLNKADANSEVTVYYQTGLKNLREHTLMELLVMHMEEPCFDFLRTKETLGYQVYPICRNTSGILGFSVTVETQATKFSTDFVEGKIEAFLVSFGEKLVQLSDEAFGAQVTALIKLKECEDTQLGDEVDRNWFEVVTQQYVFDRLNKEIEILKDVTKDELVSFYMEHRKENSRKLSIHVVGFGEEEKEIHGEDQCSSNPETSSYANISKLTFLSAAAMKEKSTMITDIRAFTSSLHLHPYHKILI

Target ORF information:

RefSeq Version	XM_009296249.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296249.2

ORF Insert Sequence:

ATGATTCCTG ATGCTGTAGA CAGAGAGGTG GAGGCTGTAG ACAGCGAGTA CCAGATGGCC 
CAGCCTTTAG ACTCAAACCG TAAAGAGATG CTGTTTGGAA GTCTAGCAAA AGCCGGTCAT 
CCGATGAGCA AGTTCTTCTG GGGAAATGCT CAAACACTAA AACAAGAGCC GAGAGAGAAG 
AAAATCAACA CTTATGAGCG TCTGAGAGAC TTCTGGAGGA GATATTACTC TGCACAATAC 
ATGACACTTG CTGTCCAATC CAAAGAGACA CTTGATACGC TGGAGGAATG GGTGCGAGAG 
ATCTTCGTTC AGATTCCTAA CAATGGTCTG CCAAAGGCTG ATTTCTCAGA CCTTCAGGAC 
CCGTTCGACA CCCCAGATTT TTGCAAACTG TACAGAGTGG TGCCTGTGCA AAAGGTTCAC 
GCTCTGACCA TCAGCTGGGC TCTTCCACCT CAGGCCAAAC ATTACAGAGT GAAACCCTTG 
CATTATATTT CCTGGCTGAT CGGTCATGAA GGAGTGGGAA GTGTCCTGTC TTTACTCAGA 
AAGAGGTGTT GGGCTCTGAG TCTGTTTGGA GGAAACAGTG AGTCGGGCTT TGATCAAAAC 
TCCACGTACT CCATCTTTAG CATTTCTATC ACTTTGAGCG ACGAAGGTCT TCAGAACTTC 
CTCCAGGTCA TTCATATCAT CTTCCAGTAT CTGAAGATGC TTCAGTCTGT TGGACCTCAG 
CAGAGGATCT ACGAGGAGAT CCAGAAGATT GAAGCCAATG AGTTTCACTA TCAAGAGCAG 
ACAGAGCCAA TTGAGTTTGT GGCGAACATG AGTGAGAACA TGCAGCTGTT CCCGAAAGAG 
CACTTCCTGT GTGGAGACCA GCTGATGTTT GACTTCAACC CAGAGGTCAT TTCAGCTGCT 
TTGTCACTCT TGACTCCAGG AAAGGCTAAT ATTTTACTGC TGTCACCTCA GCATGATGGT 
CTCTGCCCAC TCAAGGAGAA GTGGTTCGGG ACACAGTATA GTGTGGAAGA TATTCCTCAG 
GAATTCCGTG ATCTTTGGGC TGGAGATTTT CCTCTGCATC CTGAGCTTCA GCTCCCAGCT 
GAGAACAAGT TTATAGCCAC GGATTTCACC CTCAGAACAT CAGATTGTCC TGATACAGAC 
TTCCCAGTCA AAATAATAGA CAACGAGAGA GGCCGTCTGT GGTTTAGAAA AGACAACAAG 
TTCAAGATAC CCAAAGCTTA TGCCCGCTTC CAGCTGCTCA CTCCATTCAT ACAGGAATCA 
CCTAAAAATT TGGTCCTGTT TGATCTCTTC GTGAATATTG TGGCTCATAA TCTGGCAGAG 
CTGGCATATG ACGCTGAAGT GGCTCAACTA CAGTATAATC TACTGCCAGG AGATCACGGC 
CTGTTCATCA GACTCAAAGG ATTCAACCAC AAACTGCCTT TGCTCCTGAA GCTGATTGTA 
GATCACCTGG CCGACTTCAG CGCCACTCCA GACGTGTTTA ACATGTTCAT TGAGCAGCTG 
AAGAAAACCT ACTACATCAT CCTCATTAGA CCAGAACGCC TTGGAAAAGA TGTTCGGCTG 
CAGATTTTGG AGCACCATCG CTGGTCTGTG ATGCAGAAAT ATGAAGCCAT CATGGCGGAT 
CCGAGTGTGG CTGATCTCAT GACCTTTGCC AATCGCTTTA AGGCTGAGTT ATTTGTGGAA 
GGACTCGTAC AAGGGAACTT CACAAGTGCT GAGTCCAAAG AGTTTCTGCA GTGTTTTATT 
GAGAAACTCA AGTATGCACC TCATCCCATT GAACCTCCTG TGCTGTTTCG AGTGGTGGAG 
TTACCACAAA CACACCACTT ATGTAAAGTC CAGTCGCTCA ATAAAGCAGA CGCAAACTCT 
GAAGTCACCG TATACTATCA GACTGGGCTG AAGAACTTGC GAGAGCATAC ACTGATGGAG 
CTGCTTGTTA TGCACATGGA AGAGCCCTGC TTTGATTTCC TGAGGACAAA GGAAACTCTA 
GGGTATCAGG TCTATCCCAT ATGCAGAAAC ACATCCGGCA TTCTTGGCTT CTCCGTTACT 
GTCGAAACTC AGGCCACCAA ATTCAGCACG GACTTTGTGG AAGGTAAAAT CGAAGCATTT 
CTGGTCAGCT TTGGAGAGAA GTTGGTTCAG CTGTCAGACG AGGCGTTTGG AGCTCAAGTG 
ACAGCGCTGA TCAAGCTGAA GGAGTGTGAA GACACACAGC TCGGGGACGA AGTGGACAGA 
AACTGGTTCG AAGTGGTCAC TCAGCAGTAT GTCTTTGACA GGCTCAATAA AGAAATTGAA 
ATCCTGAAAG ACGTCACCAA AGATGAACTC GTATCATTTT ACATGGAGCA CCGAAAAGAA 
AACAGTAGAA AACTAAGCAT ACATGTCGTT GGATTCGGAG AAGAAGAGAA GGAAATCCAT 
GGAGAAGACC AGTGCAGTTC AAACCCAGAG ACTTCTTCAT ATGCAAATAT TTCCAAACTC 
ACTTTCCTCT CAGCGGCGGC CATGAAGGAG AAGTCCACCA TGATAACAGA CATCCGCGCT 
TTCACATCTT CACTCCATCT GCACCCCTAC CACAAAATCC TGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRD1B ( NM_001044715.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001038180.2 Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), mRNA.

Price & Availability↑

CloneID	ODa02234
Clone ID Related Accession (Same CDS sequence)	NM_001044715.2
Accession Version	NM_001044715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	nardilysin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR381682.7. On Jan 20, 2007 this sequence version replaced NM_001044715.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGCATCACAT 
TGTGTCATTT CAGCTGCTTT GTCACTCTTG ACTCCAGGAA AGGCTAATAT TTTACTGCTG 
TCACCTCAGC ATGATGGTCT CTGCCCACTC AAGGAGAAGT GGTTCGGGAC ACAGTATAGT 
GTGGAAGATA TTCCTCAGGA ATTCCGTGAT CTTTGGGCTG GAGATTTTCC TCTGCATCCT 
GAGCTTCAGC TCCCAGCTGA GAACAAGTTT ATAGCCACGG ATTTCACCCT CAGAACATCA 
GATTGTCCTG ATACAGACTT CCCAGTCAAA ATAATAGACA ACGAGAGAGG CCGTCTGTGG 
TTTAGAAAAG ACAACAAGTT CAAGATACCC AAAGCTTATG CCCGCTTCCA GCTGCTCACT 
CCATTCATAC AGGAATCACC TAAAAATTTG GTCCTGTTTG ATCTCTTCGT GAATATTGTG 
GCTCATAATC TGGCAGAGCT GGCATATGAC GCTGAAGTGG CTCAACTACA GTATAATCTA 
CTGCCAGGAG ATCACGGCCT GTTCATCAGA CTCAAAGGAT TCAACCACAA ACTGCCTTTG 
CTCCTGAAGC TGATTGTAGA TCACCTGGCC GACTTCAGCG CCACTCCAGA CGTGTTTAAC 
ATGTTCATTG AGCAGCTGAA GAAAACCTAC TACATCATCC TCATTAGACC AGAACGCCTT 
GGAAAAGATG TTCGGCTGCA GATTTTGGAG CACCATCGCT GGTCTGTGAT GCAGAAATAT 
GAAGCCATCA TGGCGGATCC GAGTGTGGCT GATCTCATGA CCTTTGCCAA TCGCTTTAAG 
GCTGAGTTAT TTGTGGAAGG ACTCGTACAA GGGAACTTCA CAAGTGCTGA GTCCAAAGAG 
TTTCTGCAGT GTTTTATTGA GAAACTCAAG TATGCACCTC ATCCCATTGA ACCTCCTGTG 
CTGTTTCGAG TGGTGGAGTT ACCACAAACA CACCACTTAT GTAAAGTCCA GTCGCTCAAT 
AAAGCAGACG CAAACTCTGA AGTCACCGTA TACTATCAGA CTGGGCTGAA GAACTTGCGA 
GAGCATACAC TGATGGAGCT GCTTGTTATG CACATGGAAG AGCCCTGCTT TGATTTCCTG 
AGGACAAAGG AAACTCTAGG GTATCAGGTC TATCCCATAT GCAGAAACAC ATCCGGCATT 
CTTGGCTTCT CCGTTACTGT CGAAACTCAG GCCACCAAAT TCAGCACGGA CTTTGTGGAA 
GGTAAAATCG AAGCATTTCT GGTCAGCTTT GGAGAGAAGT TGGTTCAGCT GTCAGACGAG 
GCGTTTGGAG CTCAAGTGAC AGCGCTGATC AAGCTGAAGG AGTGTGAAGA CACACAGCTC 
GGGGACGAAG TGGACAGAAA CTGGTTCGAA GTGGTCACTC AGCAGTATGT CTTTGACAGG 
CTCAATAAAG AAATTGAAAT CCTGAAAGAC GTCACCAAAG ATGAACTCGT ATCATTTTAC 
ATGGAGCACC GAAAAGAAAA CAGTAGAAAA CTAAGCATAC ATGTCGTTGG ATTCGGAGAA 
GAAGAGAAGG AAATCCATGG AGAAGACCAG TGCAGTTCAA ACCCAGAGAC TTCTTCATAT 
GCAAATATTT CCAAACTCAC TTTCCTCTCA GCGGCGGCCA TGAAGGAGAA GTCCACCATG 
ATAACAGACA TCCGCGCTTT CACATCTTCA CTCCATCTGC ACCCCTACCA CAAAATCCTG 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038180.2
CDS	41..3226
Translation	MPQMSKAKSCGCNVTCEVSGPDSGEPPPAVTEPQESDAADSNTIIKSPNDYKTYRYIELSNGLKALLISDVSSQSESCRESVDKEVEEEGDRGSASDISKHSERGKQSCRSEKQSAAALCISVGSFSDPADLPGLAHFLEHMVFMGSEKYPVENGFDAFLKKHGGSDNASTDCERTIFQFDVQRKYLREALDRWAQFFICPLMIPDAVDREVEAVDSEYQMAQPLDSNRKEMLFGSLAKAGHPMSKFFWGNAQTLKQEPREKKINTYERLRDFWRRYYSAQYMTLAVQSKETLDTLEEWVREIFVQIPNNGLPKADFSDLQDPFDTPDFCKLYRVVPVQKVHALTISWALPPQAKHYRVKPLHYISWLIGHEGVGSVLSLLRKRCWALSLFGGNSESGFDQNSTYSIFSISITLSDEGLQNFLQVIHIIFQYLKMLQSVGPQQRIYEEIQKIEANEFHYQEQTEPIEFVANMSENMQLFPKEHFLCGDQLMFDFNPEASHCVISAALSLLTPGKANILLLSPQHDGLCPLKEKWFGTQYSVEDIPQEFRDLWAGDFPLHPELQLPAENKFIATDFTLRTSDCPDTDFPVKIIDNERGRLWFRKDNKFKIPKAYARFQLLTPFIQESPKNLVLFDLFVNIVAHNLAELAYDAEVAQLQYNLLPGDHGLFIRLKGFNHKLPLLLKLIVDHLADFSATPDVFNMFIEQLKKTYYIILIRPERLGKDVRLQILEHHRWSVMQKYEAIMADPSVADLMTFANRFKAELFVEGLVQGNFTSAESKEFLQCFIEKLKYAPHPIEPPVLFRVVELPQTHHLCKVQSLNKADANSEVTVYYQTGLKNLREHTLMELLVMHMEEPCFDFLRTKETLGYQVYPICRNTSGILGFSVTVETQATKFSTDFVEGKIEAFLVSFGEKLVQLSDEAFGAQVTALIKLKECEDTQLGDEVDRNWFEVVTQQYVFDRLNKEIEILKDVTKDELVSFYMEHRKENSRKLSIHVVGFGEEEKEIHGEDQCSSNPETSSYANISKLTFLSAAAMKEKSTMITDIRAFTSSLHLHPYHKILI

Target ORF information:

RefSeq Version	NM_001044715.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044715.2

ORF Insert Sequence:

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGCATCACAT 
TGTGTCATTT CAGCTGCTTT GTCACTCTTG ACTCCAGGAA AGGCTAATAT TTTACTGCTG 
TCACCTCAGC ATGATGGTCT CTGCCCACTC AAGGAGAAGT GGTTCGGGAC ACAGTATAGT 
GTGGAAGATA TTCCTCAGGA ATTCCGTGAT CTTTGGGCTG GAGATTTTCC TCTGCATCCT 
GAGCTTCAGC TCCCAGCTGA GAACAAGTTT ATAGCCACGG ATTTCACCCT CAGAACATCA 
GATTGTCCTG ATACAGACTT CCCAGTCAAA ATAATAGACA ACGAGAGAGG CCGTCTGTGG 
TTTAGAAAAG ACAACAAGTT CAAGATACCC AAAGCTTATG CCCGCTTCCA GCTGCTCACT 
CCATTCATAC AGGAATCACC TAAAAATTTG GTCCTGTTTG ATCTCTTCGT GAATATTGTG 
GCTCATAATC TGGCAGAGCT GGCATATGAC GCTGAAGTGG CTCAACTACA GTATAATCTA 
CTGCCAGGAG ATCACGGCCT GTTCATCAGA CTCAAAGGAT TCAACCACAA ACTGCCTTTG 
CTCCTGAAGC TGATTGTAGA TCACCTGGCC GACTTCAGCG CCACTCCAGA CGTGTTTAAC 
ATGTTCATTG AGCAGCTGAA GAAAACCTAC TACATCATCC TCATTAGACC AGAACGCCTT 
GGAAAAGATG TTCGGCTGCA GATTTTGGAG CACCATCGCT GGTCTGTGAT GCAGAAATAT 
GAAGCCATCA TGGCGGATCC GAGTGTGGCT GATCTCATGA CCTTTGCCAA TCGCTTTAAG 
GCTGAGTTAT TTGTGGAAGG ACTCGTACAA GGGAACTTCA CAAGTGCTGA GTCCAAAGAG 
TTTCTGCAGT GTTTTATTGA GAAACTCAAG TATGCACCTC ATCCCATTGA ACCTCCTGTG 
CTGTTTCGAG TGGTGGAGTT ACCACAAACA CACCACTTAT GTAAAGTCCA GTCGCTCAAT 
AAAGCAGACG CAAACTCTGA AGTCACCGTA TACTATCAGA CTGGGCTGAA GAACTTGCGA 
GAGCATACAC TGATGGAGCT GCTTGTTATG CACATGGAAG AGCCCTGCTT TGATTTCCTG 
AGGACAAAGG AAACTCTAGG GTATCAGGTC TATCCCATAT GCAGAAACAC ATCCGGCATT 
CTTGGCTTCT CCGTTACTGT CGAAACTCAG GCCACCAAAT TCAGCACGGA CTTTGTGGAA 
GGTAAAATCG AAGCATTTCT GGTCAGCTTT GGAGAGAAGT TGGTTCAGCT GTCAGACGAG 
GCGTTTGGAG CTCAAGTGAC AGCGCTGATC AAGCTGAAGG AGTGTGAAGA CACACAGCTC 
GGGGACGAAG TGGACAGAAA CTGGTTCGAA GTGGTCACTC AGCAGTATGT CTTTGACAGG 
CTCAATAAAG AAATTGAAAT CCTGAAAGAC GTCACCAAAG ATGAACTCGT ATCATTTTAC 
ATGGAGCACC GAAAAGAAAA CAGTAGAAAA CTAAGCATAC ATGTCGTTGG ATTCGGAGAA 
GAAGAGAAGG AAATCCATGG AGAAGACCAG TGCAGTTCAA ACCCAGAGAC TTCTTCATAT 
GCAAATATTT CCAAACTCAC TTTCCTCTCA GCGGCGGCCA TGAAGGAGAA GTCCACCATG 
ATAACAGACA TCCGCGCTTT CACATCTTCA CTCCATCTGC ACCCCTACCA CAAAATCCTG 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRD1B ( XM_009296247.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294522.1 Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42629
Clone ID Related Accession (Same CDS sequence)	XM_009296247.2 , XM_009296248.2 , XM_009296248.1 , XM_009296247.1
Accession Version	XM_009296247.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nardilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGTCATTTCA 
GCTGCTTTGT CACTCTTGAC TCCAGGAAAG GCTAATATTT TACTGCTGTC ACCTCAGCAT 
GATGGTCTCT GCCCACTCAA GGAGAAGTGG TTCGGGACAC AGTATAGTGT GGAAGATATT 
CCTCAGGAAT TCCGTGATCT TTGGGCTGGA GATTTTCCTC TGCATCCTGA GCTTCAGCTC 
CCAGCTGAGA ACAAGTTTAT AGCCACGGAT TTCACCCTCA GAACATCAGA TTGTCCTGAT 
ACAGACTTCC CAGTCAAAAT AATAGACAAC GAGAGAGGCC GTCTGTGGTT TAGAAAAGAC 
AACAAGTTCA AGATACCCAA AGCTTATGCC CGCTTCCAGC TGCTCACTCC ATTCATACAG 
GAATCACCTA AAAATTTGGT CCTGTTTGAT CTCTTCGTGA ATATTGTGGC TCATAATCTG 
GCAGAGCTGG CATATGACGC TGAAGTGGCT CAACTACAGT ATAATCTACT GCCAGGAGAT 
CACGGCCTGT TCATCAGACT CAAAGGATTC AACCACAAAC TGCCTTTGCT CCTGAAGCTG 
ATTGTAGATC ACCTGGCCGA CTTCAGCGCC ACTCCAGACG TGTTTAACAT GTTCATTGAG 
CAGCTGAAGA AAACCTACTA CATCATCCTC ATTAGACCAG AACGCCTTGG AAAAGATGTT 
CGGCTGCAGA TTTTGGAGCA CCATCGCTGG TCTGTGATGC AGAAATATGA AGCCATCATG 
GCGGATCCGA GTGTGGCTGA TCTCATGACC TTTGCCAATC GCTTTAAGGC TGAGTTATTT 
GTGGAAGGAC TCGTACAAGG GAACTTCACA AGTGCTGAGT CCAAAGAGTT TCTGCAGTGT 
TTTATTGAGA AACTCAAGTA TGCACCTCAT CCCATTGAAC CTCCTGTGCT GTTTCGAGTG 
GTGGAGTTAC CACAAACACA CCACTTATGT AAAGTCCAGT CGCTCAATAA AGCAGACGCA 
AACTCTGAAG TCACCGTATA CTATCAGACT GGGCTGAAGA ACTTGCGAGA GCATACACTG 
ATGGAGCTGC TTGTTATGCA CATGGAAGAG CCCTGCTTTG ATTTCCTGAG GACAAAGGAA 
ACTCTAGGGT ATCAGGTCTA TCCCATATGC AGAAACACAT CCGGCATTCT TGGCTTCTCC 
GTTACTGTCG AAACTCAGGC CACCAAATTC AGCACGGACT TTGTGGAAGG TAAAATCGAA 
GCATTTCTGG TCAGCTTTGG AGAGAAGTTG GTTCAGCTGT CAGACGAGGC GTTTGGAGCT 
CAAGTGACAG CGCTGATCAA GCTGAAGGAG TGTGAAGACA CACAGCTCGG GGACGAAGTG 
GACAGAAACT GGTTCGAAGT GGTCACTCAG CAGTATGTCT TTGACAGGCT CAATAAAGAA 
ATTGAAATCC TGAAAGACGT CACCAAAGAT GAACTCGTAT CATTTTACAT GGAGCACCGA 
AAAGAAAACA GTAGAAAACT AAGCATACAT GTCGTTGGAT TCGGAGAAGA AGAGAAGGAA 
ATCCATGGAG AAGACCAGTG CAGTTCAAAC CCAGAGACTT CTTCATATGC AAATATTTCC 
AAACTCACTT TCCTCTCAGC GGCGGCCATG AAGGAGAAGT CCACCATGAT AACAGACATC 
CGCGCTTTCA CATCTTCACT CCATCTGCAC CCCTACCACA AAATCCTGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294522.1
CDS	98..3271
Translation	MPQMSKAKSCGCNVTCEVSGPDSGEPPPAVTEPQESDAADSNTIIKSPNDYKTYRYIELSNGLKALLISDVSSQSESCRESVDKEVEEEGDRGSASDISKHSERGKQSCRSEKQSAAALCISVGSFSDPADLPGLAHFLEHMVFMGSEKYPVENGFDAFLKKHGGSDNASTDCERTIFQFDVQRKYLREALDRWAQFFICPLMIPDAVDREVEAVDSEYQMAQPLDSNRKEMLFGSLAKAGHPMSKFFWGNAQTLKQEPREKKINTYERLRDFWRRYYSAQYMTLAVQSKETLDTLEEWVREIFVQIPNNGLPKADFSDLQDPFDTPDFCKLYRVVPVQKVHALTISWALPPQAKHYRVKPLHYISWLIGHEGVGSVLSLLRKRCWALSLFGGNSESGFDQNSTYSIFSISITLSDEGLQNFLQVIHIIFQYLKMLQSVGPQQRIYEEIQKIEANEFHYQEQTEPIEFVANMSENMQLFPKEHFLCGDQLMFDFNPEVISAALSLLTPGKANILLLSPQHDGLCPLKEKWFGTQYSVEDIPQEFRDLWAGDFPLHPELQLPAENKFIATDFTLRTSDCPDTDFPVKIIDNERGRLWFRKDNKFKIPKAYARFQLLTPFIQESPKNLVLFDLFVNIVAHNLAELAYDAEVAQLQYNLLPGDHGLFIRLKGFNHKLPLLLKLIVDHLADFSATPDVFNMFIEQLKKTYYIILIRPERLGKDVRLQILEHHRWSVMQKYEAIMADPSVADLMTFANRFKAELFVEGLVQGNFTSAESKEFLQCFIEKLKYAPHPIEPPVLFRVVELPQTHHLCKVQSLNKADANSEVTVYYQTGLKNLREHTLMELLVMHMEEPCFDFLRTKETLGYQVYPICRNTSGILGFSVTVETQATKFSTDFVEGKIEAFLVSFGEKLVQLSDEAFGAQVTALIKLKECEDTQLGDEVDRNWFEVVTQQYVFDRLNKEIEILKDVTKDELVSFYMEHRKENSRKLSIHVVGFGEEEKEIHGEDQCSSNPETSSYANISKLTFLSAAAMKEKSTMITDIRAFTSSLHLHPYHKILI

Target ORF information:

RefSeq Version	XM_009296247.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296247.2

ORF Insert Sequence:

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGTCATTTCA 
GCTGCTTTGT CACTCTTGAC TCCAGGAAAG GCTAATATTT TACTGCTGTC ACCTCAGCAT 
GATGGTCTCT GCCCACTCAA GGAGAAGTGG TTCGGGACAC AGTATAGTGT GGAAGATATT 
CCTCAGGAAT TCCGTGATCT TTGGGCTGGA GATTTTCCTC TGCATCCTGA GCTTCAGCTC 
CCAGCTGAGA ACAAGTTTAT AGCCACGGAT TTCACCCTCA GAACATCAGA TTGTCCTGAT 
ACAGACTTCC CAGTCAAAAT AATAGACAAC GAGAGAGGCC GTCTGTGGTT TAGAAAAGAC 
AACAAGTTCA AGATACCCAA AGCTTATGCC CGCTTCCAGC TGCTCACTCC ATTCATACAG 
GAATCACCTA AAAATTTGGT CCTGTTTGAT CTCTTCGTGA ATATTGTGGC TCATAATCTG 
GCAGAGCTGG CATATGACGC TGAAGTGGCT CAACTACAGT ATAATCTACT GCCAGGAGAT 
CACGGCCTGT TCATCAGACT CAAAGGATTC AACCACAAAC TGCCTTTGCT CCTGAAGCTG 
ATTGTAGATC ACCTGGCCGA CTTCAGCGCC ACTCCAGACG TGTTTAACAT GTTCATTGAG 
CAGCTGAAGA AAACCTACTA CATCATCCTC ATTAGACCAG AACGCCTTGG AAAAGATGTT 
CGGCTGCAGA TTTTGGAGCA CCATCGCTGG TCTGTGATGC AGAAATATGA AGCCATCATG 
GCGGATCCGA GTGTGGCTGA TCTCATGACC TTTGCCAATC GCTTTAAGGC TGAGTTATTT 
GTGGAAGGAC TCGTACAAGG GAACTTCACA AGTGCTGAGT CCAAAGAGTT TCTGCAGTGT 
TTTATTGAGA AACTCAAGTA TGCACCTCAT CCCATTGAAC CTCCTGTGCT GTTTCGAGTG 
GTGGAGTTAC CACAAACACA CCACTTATGT AAAGTCCAGT CGCTCAATAA AGCAGACGCA 
AACTCTGAAG TCACCGTATA CTATCAGACT GGGCTGAAGA ACTTGCGAGA GCATACACTG 
ATGGAGCTGC TTGTTATGCA CATGGAAGAG CCCTGCTTTG ATTTCCTGAG GACAAAGGAA 
ACTCTAGGGT ATCAGGTCTA TCCCATATGC AGAAACACAT CCGGCATTCT TGGCTTCTCC 
GTTACTGTCG AAACTCAGGC CACCAAATTC AGCACGGACT TTGTGGAAGG TAAAATCGAA 
GCATTTCTGG TCAGCTTTGG AGAGAAGTTG GTTCAGCTGT CAGACGAGGC GTTTGGAGCT 
CAAGTGACAG CGCTGATCAA GCTGAAGGAG TGTGAAGACA CACAGCTCGG GGACGAAGTG 
GACAGAAACT GGTTCGAAGT GGTCACTCAG CAGTATGTCT TTGACAGGCT CAATAAAGAA 
ATTGAAATCC TGAAAGACGT CACCAAAGAT GAACTCGTAT CATTTTACAT GGAGCACCGA 
AAAGAAAACA GTAGAAAACT AAGCATACAT GTCGTTGGAT TCGGAGAAGA AGAGAAGGAA 
ATCCATGGAG AAGACCAGTG CAGTTCAAAC CCAGAGACTT CTTCATATGC AAATATTTCC 
AAACTCACTT TCCTCTCAGC GGCGGCCATG AAGGAGAAGT CCACCATGAT AACAGACATC 
CGCGCTTTCA CATCTTCACT CCATCTGCAC CCCTACCACA AAATCCTGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRD1B ( XM_009296248.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294523.1 Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42629
Clone ID Related Accession (Same CDS sequence)	XM_009296247.2 , XM_009296248.2 , XM_009296248.1 , XM_009296247.1
Accession Version	XM_009296248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nardilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGTCATTTCA 
GCTGCTTTGT CACTCTTGAC TCCAGGAAAG GCTAATATTT TACTGCTGTC ACCTCAGCAT 
GATGGTCTCT GCCCACTCAA GGAGAAGTGG TTCGGGACAC AGTATAGTGT GGAAGATATT 
CCTCAGGAAT TCCGTGATCT TTGGGCTGGA GATTTTCCTC TGCATCCTGA GCTTCAGCTC 
CCAGCTGAGA ACAAGTTTAT AGCCACGGAT TTCACCCTCA GAACATCAGA TTGTCCTGAT 
ACAGACTTCC CAGTCAAAAT AATAGACAAC GAGAGAGGCC GTCTGTGGTT TAGAAAAGAC 
AACAAGTTCA AGATACCCAA AGCTTATGCC CGCTTCCAGC TGCTCACTCC ATTCATACAG 
GAATCACCTA AAAATTTGGT CCTGTTTGAT CTCTTCGTGA ATATTGTGGC TCATAATCTG 
GCAGAGCTGG CATATGACGC TGAAGTGGCT CAACTACAGT ATAATCTACT GCCAGGAGAT 
CACGGCCTGT TCATCAGACT CAAAGGATTC AACCACAAAC TGCCTTTGCT CCTGAAGCTG 
ATTGTAGATC ACCTGGCCGA CTTCAGCGCC ACTCCAGACG TGTTTAACAT GTTCATTGAG 
CAGCTGAAGA AAACCTACTA CATCATCCTC ATTAGACCAG AACGCCTTGG AAAAGATGTT 
CGGCTGCAGA TTTTGGAGCA CCATCGCTGG TCTGTGATGC AGAAATATGA AGCCATCATG 
GCGGATCCGA GTGTGGCTGA TCTCATGACC TTTGCCAATC GCTTTAAGGC TGAGTTATTT 
GTGGAAGGAC TCGTACAAGG GAACTTCACA AGTGCTGAGT CCAAAGAGTT TCTGCAGTGT 
TTTATTGAGA AACTCAAGTA TGCACCTCAT CCCATTGAAC CTCCTGTGCT GTTTCGAGTG 
GTGGAGTTAC CACAAACACA CCACTTATGT AAAGTCCAGT CGCTCAATAA AGCAGACGCA 
AACTCTGAAG TCACCGTATA CTATCAGACT GGGCTGAAGA ACTTGCGAGA GCATACACTG 
ATGGAGCTGC TTGTTATGCA CATGGAAGAG CCCTGCTTTG ATTTCCTGAG GACAAAGGAA 
ACTCTAGGGT ATCAGGTCTA TCCCATATGC AGAAACACAT CCGGCATTCT TGGCTTCTCC 
GTTACTGTCG AAACTCAGGC CACCAAATTC AGCACGGACT TTGTGGAAGG TAAAATCGAA 
GCATTTCTGG TCAGCTTTGG AGAGAAGTTG GTTCAGCTGT CAGACGAGGC GTTTGGAGCT 
CAAGTGACAG CGCTGATCAA GCTGAAGGAG TGTGAAGACA CACAGCTCGG GGACGAAGTG 
GACAGAAACT GGTTCGAAGT GGTCACTCAG CAGTATGTCT TTGACAGGCT CAATAAAGAA 
ATTGAAATCC TGAAAGACGT CACCAAAGAT GAACTCGTAT CATTTTACAT GGAGCACCGA 
AAAGAAAACA GTAGAAAACT AAGCATACAT GTCGTTGGAT TCGGAGAAGA AGAGAAGGAA 
ATCCATGGAG AAGACCAGTG CAGTTCAAAC CCAGAGACTT CTTCATATGC AAATATTTCC 
AAACTCACTT TCCTCTCAGC GGCGGCCATG AAGGAGAAGT CCACCATGAT AACAGACATC 
CGCGCTTTCA CATCTTCACT CCATCTGCAC CCCTACCACA AAATCCTGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294523.1
CDS	120..3293
Translation	MPQMSKAKSCGCNVTCEVSGPDSGEPPPAVTEPQESDAADSNTIIKSPNDYKTYRYIELSNGLKALLISDVSSQSESCRESVDKEVEEEGDRGSASDISKHSERGKQSCRSEKQSAAALCISVGSFSDPADLPGLAHFLEHMVFMGSEKYPVENGFDAFLKKHGGSDNASTDCERTIFQFDVQRKYLREALDRWAQFFICPLMIPDAVDREVEAVDSEYQMAQPLDSNRKEMLFGSLAKAGHPMSKFFWGNAQTLKQEPREKKINTYERLRDFWRRYYSAQYMTLAVQSKETLDTLEEWVREIFVQIPNNGLPKADFSDLQDPFDTPDFCKLYRVVPVQKVHALTISWALPPQAKHYRVKPLHYISWLIGHEGVGSVLSLLRKRCWALSLFGGNSESGFDQNSTYSIFSISITLSDEGLQNFLQVIHIIFQYLKMLQSVGPQQRIYEEIQKIEANEFHYQEQTEPIEFVANMSENMQLFPKEHFLCGDQLMFDFNPEVISAALSLLTPGKANILLLSPQHDGLCPLKEKWFGTQYSVEDIPQEFRDLWAGDFPLHPELQLPAENKFIATDFTLRTSDCPDTDFPVKIIDNERGRLWFRKDNKFKIPKAYARFQLLTPFIQESPKNLVLFDLFVNIVAHNLAELAYDAEVAQLQYNLLPGDHGLFIRLKGFNHKLPLLLKLIVDHLADFSATPDVFNMFIEQLKKTYYIILIRPERLGKDVRLQILEHHRWSVMQKYEAIMADPSVADLMTFANRFKAELFVEGLVQGNFTSAESKEFLQCFIEKLKYAPHPIEPPVLFRVVELPQTHHLCKVQSLNKADANSEVTVYYQTGLKNLREHTLMELLVMHMEEPCFDFLRTKETLGYQVYPICRNTSGILGFSVTVETQATKFSTDFVEGKIEAFLVSFGEKLVQLSDEAFGAQVTALIKLKECEDTQLGDEVDRNWFEVVTQQYVFDRLNKEIEILKDVTKDELVSFYMEHRKENSRKLSIHVVGFGEEEKEIHGEDQCSSNPETSSYANISKLTFLSAAAMKEKSTMITDIRAFTSSLHLHPYHKILI

Target ORF information:

RefSeq Version	XM_009296248.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296248.2

ORF Insert Sequence:

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGTCATTTCA 
GCTGCTTTGT CACTCTTGAC TCCAGGAAAG GCTAATATTT TACTGCTGTC ACCTCAGCAT 
GATGGTCTCT GCCCACTCAA GGAGAAGTGG TTCGGGACAC AGTATAGTGT GGAAGATATT 
CCTCAGGAAT TCCGTGATCT TTGGGCTGGA GATTTTCCTC TGCATCCTGA GCTTCAGCTC 
CCAGCTGAGA ACAAGTTTAT AGCCACGGAT TTCACCCTCA GAACATCAGA TTGTCCTGAT 
ACAGACTTCC CAGTCAAAAT AATAGACAAC GAGAGAGGCC GTCTGTGGTT TAGAAAAGAC 
AACAAGTTCA AGATACCCAA AGCTTATGCC CGCTTCCAGC TGCTCACTCC ATTCATACAG 
GAATCACCTA AAAATTTGGT CCTGTTTGAT CTCTTCGTGA ATATTGTGGC TCATAATCTG 
GCAGAGCTGG CATATGACGC TGAAGTGGCT CAACTACAGT ATAATCTACT GCCAGGAGAT 
CACGGCCTGT TCATCAGACT CAAAGGATTC AACCACAAAC TGCCTTTGCT CCTGAAGCTG 
ATTGTAGATC ACCTGGCCGA CTTCAGCGCC ACTCCAGACG TGTTTAACAT GTTCATTGAG 
CAGCTGAAGA AAACCTACTA CATCATCCTC ATTAGACCAG AACGCCTTGG AAAAGATGTT 
CGGCTGCAGA TTTTGGAGCA CCATCGCTGG TCTGTGATGC AGAAATATGA AGCCATCATG 
GCGGATCCGA GTGTGGCTGA TCTCATGACC TTTGCCAATC GCTTTAAGGC TGAGTTATTT 
GTGGAAGGAC TCGTACAAGG GAACTTCACA AGTGCTGAGT CCAAAGAGTT TCTGCAGTGT 
TTTATTGAGA AACTCAAGTA TGCACCTCAT CCCATTGAAC CTCCTGTGCT GTTTCGAGTG 
GTGGAGTTAC CACAAACACA CCACTTATGT AAAGTCCAGT CGCTCAATAA AGCAGACGCA 
AACTCTGAAG TCACCGTATA CTATCAGACT GGGCTGAAGA ACTTGCGAGA GCATACACTG 
ATGGAGCTGC TTGTTATGCA CATGGAAGAG CCCTGCTTTG ATTTCCTGAG GACAAAGGAA 
ACTCTAGGGT ATCAGGTCTA TCCCATATGC AGAAACACAT CCGGCATTCT TGGCTTCTCC 
GTTACTGTCG AAACTCAGGC CACCAAATTC AGCACGGACT TTGTGGAAGG TAAAATCGAA 
GCATTTCTGG TCAGCTTTGG AGAGAAGTTG GTTCAGCTGT CAGACGAGGC GTTTGGAGCT 
CAAGTGACAG CGCTGATCAA GCTGAAGGAG TGTGAAGACA CACAGCTCGG GGACGAAGTG 
GACAGAAACT GGTTCGAAGT GGTCACTCAG CAGTATGTCT TTGACAGGCT CAATAAAGAA 
ATTGAAATCC TGAAAGACGT CACCAAAGAT GAACTCGTAT CATTTTACAT GGAGCACCGA 
AAAGAAAACA GTAGAAAACT AAGCATACAT GTCGTTGGAT TCGGAGAAGA AGAGAAGGAA 
ATCCATGGAG AAGACCAGTG CAGTTCAAAC CCAGAGACTT CTTCATATGC AAATATTTCC 
AAACTCACTT TCCTCTCAGC GGCGGCCATG AAGGAGAAGT CCACCATGAT AACAGACATC 
CGCGCTTTCA CATCTTCACT CCATCTGCAC CCCTACCACA AAATCCTGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRD1B ( XM_009296248.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294523.1 Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42629
Clone ID Related Accession (Same CDS sequence)	XM_009296247.2 , XM_009296248.2 , XM_009296248.1 , XM_009296247.1
Accession Version	XM_009296248.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nardilysin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGTCATTTCA 
GCTGCTTTGT CACTCTTGAC TCCAGGAAAG GCTAATATTT TACTGCTGTC ACCTCAGCAT 
GATGGTCTCT GCCCACTCAA GGAGAAGTGG TTCGGGACAC AGTATAGTGT GGAAGATATT 
CCTCAGGAAT TCCGTGATCT TTGGGCTGGA GATTTTCCTC TGCATCCTGA GCTTCAGCTC 
CCAGCTGAGA ACAAGTTTAT AGCCACGGAT TTCACCCTCA GAACATCAGA TTGTCCTGAT 
ACAGACTTCC CAGTCAAAAT AATAGACAAC GAGAGAGGCC GTCTGTGGTT TAGAAAAGAC 
AACAAGTTCA AGATACCCAA AGCTTATGCC CGCTTCCAGC TGCTCACTCC ATTCATACAG 
GAATCACCTA AAAATTTGGT CCTGTTTGAT CTCTTCGTGA ATATTGTGGC TCATAATCTG 
GCAGAGCTGG CATATGACGC TGAAGTGGCT CAACTACAGT ATAATCTACT GCCAGGAGAT 
CACGGCCTGT TCATCAGACT CAAAGGATTC AACCACAAAC TGCCTTTGCT CCTGAAGCTG 
ATTGTAGATC ACCTGGCCGA CTTCAGCGCC ACTCCAGACG TGTTTAACAT GTTCATTGAG 
CAGCTGAAGA AAACCTACTA CATCATCCTC ATTAGACCAG AACGCCTTGG AAAAGATGTT 
CGGCTGCAGA TTTTGGAGCA CCATCGCTGG TCTGTGATGC AGAAATATGA AGCCATCATG 
GCGGATCCGA GTGTGGCTGA TCTCATGACC TTTGCCAATC GCTTTAAGGC TGAGTTATTT 
GTGGAAGGAC TCGTACAAGG GAACTTCACA AGTGCTGAGT CCAAAGAGTT TCTGCAGTGT 
TTTATTGAGA AACTCAAGTA TGCACCTCAT CCCATTGAAC CTCCTGTGCT GTTTCGAGTG 
GTGGAGTTAC CACAAACACA CCACTTATGT AAAGTCCAGT CGCTCAATAA AGCAGACGCA 
AACTCTGAAG TCACCGTATA CTATCAGACT GGGCTGAAGA ACTTGCGAGA GCATACACTG 
ATGGAGCTGC TTGTTATGCA CATGGAAGAG CCCTGCTTTG ATTTCCTGAG GACAAAGGAA 
ACTCTAGGGT ATCAGGTCTA TCCCATATGC AGAAACACAT CCGGCATTCT TGGCTTCTCC 
GTTACTGTCG AAACTCAGGC CACCAAATTC AGCACGGACT TTGTGGAAGG TAAAATCGAA 
GCATTTCTGG TCAGCTTTGG AGAGAAGTTG GTTCAGCTGT CAGACGAGGC GTTTGGAGCT 
CAAGTGACAG CGCTGATCAA GCTGAAGGAG TGTGAAGACA CACAGCTCGG GGACGAAGTG 
GACAGAAACT GGTTCGAAGT GGTCACTCAG CAGTATGTCT TTGACAGGCT CAATAAAGAA 
ATTGAAATCC TGAAAGACGT CACCAAAGAT GAACTCGTAT CATTTTACAT GGAGCACCGA 
AAAGAAAACA GTAGAAAACT AAGCATACAT GTCGTTGGAT TCGGAGAAGA AGAGAAGGAA 
ATCCATGGAG AAGACCAGTG CAGTTCAAAC CCAGAGACTT CTTCATATGC AAATATTTCC 
AAACTCACTT TCCTCTCAGC GGCGGCCATG AAGGAGAAGT CCACCATGAT AACAGACATC 
CGCGCTTTCA CATCTTCACT CCATCTGCAC CCCTACCACA AAATCCTGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294523.1
CDS	100..3273
Translation	MPQMSKAKSCGCNVTCEVSGPDSGEPPPAVTEPQESDAADSNTIIKSPNDYKTYRYIELSNGLKALLISDVSSQSESCRESVDKEVEEEGDRGSASDISKHSERGKQSCRSEKQSAAALCISVGSFSDPADLPGLAHFLEHMVFMGSEKYPVENGFDAFLKKHGGSDNASTDCERTIFQFDVQRKYLREALDRWAQFFICPLMIPDAVDREVEAVDSEYQMAQPLDSNRKEMLFGSLAKAGHPMSKFFWGNAQTLKQEPREKKINTYERLRDFWRRYYSAQYMTLAVQSKETLDTLEEWVREIFVQIPNNGLPKADFSDLQDPFDTPDFCKLYRVVPVQKVHALTISWALPPQAKHYRVKPLHYISWLIGHEGVGSVLSLLRKRCWALSLFGGNSESGFDQNSTYSIFSISITLSDEGLQNFLQVIHIIFQYLKMLQSVGPQQRIYEEIQKIEANEFHYQEQTEPIEFVANMSENMQLFPKEHFLCGDQLMFDFNPEVISAALSLLTPGKANILLLSPQHDGLCPLKEKWFGTQYSVEDIPQEFRDLWAGDFPLHPELQLPAENKFIATDFTLRTSDCPDTDFPVKIIDNERGRLWFRKDNKFKIPKAYARFQLLTPFIQESPKNLVLFDLFVNIVAHNLAELAYDAEVAQLQYNLLPGDHGLFIRLKGFNHKLPLLLKLIVDHLADFSATPDVFNMFIEQLKKTYYIILIRPERLGKDVRLQILEHHRWSVMQKYEAIMADPSVADLMTFANRFKAELFVEGLVQGNFTSAESKEFLQCFIEKLKYAPHPIEPPVLFRVVELPQTHHLCKVQSLNKADANSEVTVYYQTGLKNLREHTLMELLVMHMEEPCFDFLRTKETLGYQVYPICRNTSGILGFSVTVETQATKFSTDFVEGKIEAFLVSFGEKLVQLSDEAFGAQVTALIKLKECEDTQLGDEVDRNWFEVVTQQYVFDRLNKEIEILKDVTKDELVSFYMEHRKENSRKLSIHVVGFGEEEKEIHGEDQCSSNPETSSYANISKLTFLSAAAMKEKSTMITDIRAFTSSLHLHPYHKILI

Target ORF information:

RefSeq Version	XM_009296248.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296248.1

ORF Insert Sequence:

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGTCATTTCA 
GCTGCTTTGT CACTCTTGAC TCCAGGAAAG GCTAATATTT TACTGCTGTC ACCTCAGCAT 
GATGGTCTCT GCCCACTCAA GGAGAAGTGG TTCGGGACAC AGTATAGTGT GGAAGATATT 
CCTCAGGAAT TCCGTGATCT TTGGGCTGGA GATTTTCCTC TGCATCCTGA GCTTCAGCTC 
CCAGCTGAGA ACAAGTTTAT AGCCACGGAT TTCACCCTCA GAACATCAGA TTGTCCTGAT 
ACAGACTTCC CAGTCAAAAT AATAGACAAC GAGAGAGGCC GTCTGTGGTT TAGAAAAGAC 
AACAAGTTCA AGATACCCAA AGCTTATGCC CGCTTCCAGC TGCTCACTCC ATTCATACAG 
GAATCACCTA AAAATTTGGT CCTGTTTGAT CTCTTCGTGA ATATTGTGGC TCATAATCTG 
GCAGAGCTGG CATATGACGC TGAAGTGGCT CAACTACAGT ATAATCTACT GCCAGGAGAT 
CACGGCCTGT TCATCAGACT CAAAGGATTC AACCACAAAC TGCCTTTGCT CCTGAAGCTG 
ATTGTAGATC ACCTGGCCGA CTTCAGCGCC ACTCCAGACG TGTTTAACAT GTTCATTGAG 
CAGCTGAAGA AAACCTACTA CATCATCCTC ATTAGACCAG AACGCCTTGG AAAAGATGTT 
CGGCTGCAGA TTTTGGAGCA CCATCGCTGG TCTGTGATGC AGAAATATGA AGCCATCATG 
GCGGATCCGA GTGTGGCTGA TCTCATGACC TTTGCCAATC GCTTTAAGGC TGAGTTATTT 
GTGGAAGGAC TCGTACAAGG GAACTTCACA AGTGCTGAGT CCAAAGAGTT TCTGCAGTGT 
TTTATTGAGA AACTCAAGTA TGCACCTCAT CCCATTGAAC CTCCTGTGCT GTTTCGAGTG 
GTGGAGTTAC CACAAACACA CCACTTATGT AAAGTCCAGT CGCTCAATAA AGCAGACGCA 
AACTCTGAAG TCACCGTATA CTATCAGACT GGGCTGAAGA ACTTGCGAGA GCATACACTG 
ATGGAGCTGC TTGTTATGCA CATGGAAGAG CCCTGCTTTG ATTTCCTGAG GACAAAGGAA 
ACTCTAGGGT ATCAGGTCTA TCCCATATGC AGAAACACAT CCGGCATTCT TGGCTTCTCC 
GTTACTGTCG AAACTCAGGC CACCAAATTC AGCACGGACT TTGTGGAAGG TAAAATCGAA 
GCATTTCTGG TCAGCTTTGG AGAGAAGTTG GTTCAGCTGT CAGACGAGGC GTTTGGAGCT 
CAAGTGACAG CGCTGATCAA GCTGAAGGAG TGTGAAGACA CACAGCTCGG GGACGAAGTG 
GACAGAAACT GGTTCGAAGT GGTCACTCAG CAGTATGTCT TTGACAGGCT CAATAAAGAA 
ATTGAAATCC TGAAAGACGT CACCAAAGAT GAACTCGTAT CATTTTACAT GGAGCACCGA 
AAAGAAAACA GTAGAAAACT AAGCATACAT GTCGTTGGAT TCGGAGAAGA AGAGAAGGAA 
ATCCATGGAG AAGACCAGTG CAGTTCAAAC CCAGAGACTT CTTCATATGC AAATATTTCC 
AAACTCACTT TCCTCTCAGC GGCGGCCATG AAGGAGAAGT CCACCATGAT AACAGACATC 
CGCGCTTTCA CATCTTCACT CCATCTGCAC CCCTACCACA AAATCCTGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRD1B ( XM_009296247.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294522.1 Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X1, mRNA.

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CloneID	ODa42629
Clone ID Related Accession (Same CDS sequence)	XM_009296247.2 , XM_009296248.2 , XM_009296248.1 , XM_009296247.1
Accession Version	XM_009296247.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nardilysin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGTCATTTCA 
GCTGCTTTGT CACTCTTGAC TCCAGGAAAG GCTAATATTT TACTGCTGTC ACCTCAGCAT 
GATGGTCTCT GCCCACTCAA GGAGAAGTGG TTCGGGACAC AGTATAGTGT GGAAGATATT 
CCTCAGGAAT TCCGTGATCT TTGGGCTGGA GATTTTCCTC TGCATCCTGA GCTTCAGCTC 
CCAGCTGAGA ACAAGTTTAT AGCCACGGAT TTCACCCTCA GAACATCAGA TTGTCCTGAT 
ACAGACTTCC CAGTCAAAAT AATAGACAAC GAGAGAGGCC GTCTGTGGTT TAGAAAAGAC 
AACAAGTTCA AGATACCCAA AGCTTATGCC CGCTTCCAGC TGCTCACTCC ATTCATACAG 
GAATCACCTA AAAATTTGGT CCTGTTTGAT CTCTTCGTGA ATATTGTGGC TCATAATCTG 
GCAGAGCTGG CATATGACGC TGAAGTGGCT CAACTACAGT ATAATCTACT GCCAGGAGAT 
CACGGCCTGT TCATCAGACT CAAAGGATTC AACCACAAAC TGCCTTTGCT CCTGAAGCTG 
ATTGTAGATC ACCTGGCCGA CTTCAGCGCC ACTCCAGACG TGTTTAACAT GTTCATTGAG 
CAGCTGAAGA AAACCTACTA CATCATCCTC ATTAGACCAG AACGCCTTGG AAAAGATGTT 
CGGCTGCAGA TTTTGGAGCA CCATCGCTGG TCTGTGATGC AGAAATATGA AGCCATCATG 
GCGGATCCGA GTGTGGCTGA TCTCATGACC TTTGCCAATC GCTTTAAGGC TGAGTTATTT 
GTGGAAGGAC TCGTACAAGG GAACTTCACA AGTGCTGAGT CCAAAGAGTT TCTGCAGTGT 
TTTATTGAGA AACTCAAGTA TGCACCTCAT CCCATTGAAC CTCCTGTGCT GTTTCGAGTG 
GTGGAGTTAC CACAAACACA CCACTTATGT AAAGTCCAGT CGCTCAATAA AGCAGACGCA 
AACTCTGAAG TCACCGTATA CTATCAGACT GGGCTGAAGA ACTTGCGAGA GCATACACTG 
ATGGAGCTGC TTGTTATGCA CATGGAAGAG CCCTGCTTTG ATTTCCTGAG GACAAAGGAA 
ACTCTAGGGT ATCAGGTCTA TCCCATATGC AGAAACACAT CCGGCATTCT TGGCTTCTCC 
GTTACTGTCG AAACTCAGGC CACCAAATTC AGCACGGACT TTGTGGAAGG TAAAATCGAA 
GCATTTCTGG TCAGCTTTGG AGAGAAGTTG GTTCAGCTGT CAGACGAGGC GTTTGGAGCT 
CAAGTGACAG CGCTGATCAA GCTGAAGGAG TGTGAAGACA CACAGCTCGG GGACGAAGTG 
GACAGAAACT GGTTCGAAGT GGTCACTCAG CAGTATGTCT TTGACAGGCT CAATAAAGAA 
ATTGAAATCC TGAAAGACGT CACCAAAGAT GAACTCGTAT CATTTTACAT GGAGCACCGA 
AAAGAAAACA GTAGAAAACT AAGCATACAT GTCGTTGGAT TCGGAGAAGA AGAGAAGGAA 
ATCCATGGAG AAGACCAGTG CAGTTCAAAC CCAGAGACTT CTTCATATGC AAATATTTCC 
AAACTCACTT TCCTCTCAGC GGCGGCCATG AAGGAGAAGT CCACCATGAT AACAGACATC 
CGCGCTTTCA CATCTTCACT CCATCTGCAC CCCTACCACA AAATCCTGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294522.1
CDS	80..3253
Translation	MPQMSKAKSCGCNVTCEVSGPDSGEPPPAVTEPQESDAADSNTIIKSPNDYKTYRYIELSNGLKALLISDVSSQSESCRESVDKEVEEEGDRGSASDISKHSERGKQSCRSEKQSAAALCISVGSFSDPADLPGLAHFLEHMVFMGSEKYPVENGFDAFLKKHGGSDNASTDCERTIFQFDVQRKYLREALDRWAQFFICPLMIPDAVDREVEAVDSEYQMAQPLDSNRKEMLFGSLAKAGHPMSKFFWGNAQTLKQEPREKKINTYERLRDFWRRYYSAQYMTLAVQSKETLDTLEEWVREIFVQIPNNGLPKADFSDLQDPFDTPDFCKLYRVVPVQKVHALTISWALPPQAKHYRVKPLHYISWLIGHEGVGSVLSLLRKRCWALSLFGGNSESGFDQNSTYSIFSISITLSDEGLQNFLQVIHIIFQYLKMLQSVGPQQRIYEEIQKIEANEFHYQEQTEPIEFVANMSENMQLFPKEHFLCGDQLMFDFNPEVISAALSLLTPGKANILLLSPQHDGLCPLKEKWFGTQYSVEDIPQEFRDLWAGDFPLHPELQLPAENKFIATDFTLRTSDCPDTDFPVKIIDNERGRLWFRKDNKFKIPKAYARFQLLTPFIQESPKNLVLFDLFVNIVAHNLAELAYDAEVAQLQYNLLPGDHGLFIRLKGFNHKLPLLLKLIVDHLADFSATPDVFNMFIEQLKKTYYIILIRPERLGKDVRLQILEHHRWSVMQKYEAIMADPSVADLMTFANRFKAELFVEGLVQGNFTSAESKEFLQCFIEKLKYAPHPIEPPVLFRVVELPQTHHLCKVQSLNKADANSEVTVYYQTGLKNLREHTLMELLVMHMEEPCFDFLRTKETLGYQVYPICRNTSGILGFSVTVETQATKFSTDFVEGKIEAFLVSFGEKLVQLSDEAFGAQVTALIKLKECEDTQLGDEVDRNWFEVVTQQYVFDRLNKEIEILKDVTKDELVSFYMEHRKENSRKLSIHVVGFGEEEKEIHGEDQCSSNPETSSYANISKLTFLSAAAMKEKSTMITDIRAFTSSLHLHPYHKILI

Target ORF information:

RefSeq Version	XM_009296247.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nardilysin b (N-arginine dibasic convertase) (nrd1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296247.1

ORF Insert Sequence:

ATGCCGCAAA TGAGTAAAGC TAAAAGTTGT GGATGTAACG TTACCTGTGA AGTGTCCGGA 
CCAGACAGCG GAGAACCTCC ACCTGCTGTA ACTGAACCGC AAGAGTCTGA TGCCGCAGAT 
TCAAACACCA TCATCAAATC ACCAAACGAC TACAAAACAT ACAGGTATAT TGAGCTGAGC 
AATGGACTCA AGGCGCTGCT CATATCAGAT GTTTCCAGCC AATCAGAGAG CTGCAGAGAG 
TCAGTGGATA AAGAGGTGGA AGAGGAAGGT GACAGAGGAT CTGCCAGTGA CATCAGTAAA 
CACAGTGAAA GAGGGAAACA GAGCTGTCGT TCAGAGAAGC AGTCTGCAGC TGCACTCTGT 
ATCAGTGTGG GAAGCTTCAG TGACCCTGCA GATCTTCCTG GACTCGCACA TTTTCTCGAA 
CACATGGTCT TCATGGGCAG TGAAAAGTAC CCAGTTGAAA ATGGCTTTGA TGCTTTCTTA 
AAAAAACATG GAGGAAGTGA CAACGCTTCC ACGGACTGTG AGCGAACCAT CTTCCAGTTT 
GATGTTCAGA GGAAATACCT CAGAGAAGCT CTGGATCGAT GGGCGCAGTT TTTCATCTGT 
CCTTTGATGA TTCCTGATGC TGTAGACAGA GAGGTGGAGG CTGTAGACAG CGAGTACCAG 
ATGGCCCAGC CTTTAGACTC AAACCGTAAA GAGATGCTGT TTGGAAGTCT AGCAAAAGCC 
GGTCATCCGA TGAGCAAGTT CTTCTGGGGA AATGCTCAAA CACTAAAACA AGAGCCGAGA 
GAGAAGAAAA TCAACACTTA TGAGCGTCTG AGAGACTTCT GGAGGAGATA TTACTCTGCA 
CAATACATGA CACTTGCTGT CCAATCCAAA GAGACACTTG ATACGCTGGA GGAATGGGTG 
CGAGAGATCT TCGTTCAGAT TCCTAACAAT GGTCTGCCAA AGGCTGATTT CTCAGACCTT 
CAGGACCCGT TCGACACCCC AGATTTTTGC AAACTGTACA GAGTGGTGCC TGTGCAAAAG 
GTTCACGCTC TGACCATCAG CTGGGCTCTT CCACCTCAGG CCAAACATTA CAGAGTGAAA 
CCCTTGCATT ATATTTCCTG GCTGATCGGT CATGAAGGAG TGGGAAGTGT CCTGTCTTTA 
CTCAGAAAGA GGTGTTGGGC TCTGAGTCTG TTTGGAGGAA ACAGTGAGTC GGGCTTTGAT 
CAAAACTCCA CGTACTCCAT CTTTAGCATT TCTATCACTT TGAGCGACGA AGGTCTTCAG 
AACTTCCTCC AGGTCATTCA TATCATCTTC CAGTATCTGA AGATGCTTCA GTCTGTTGGA 
CCTCAGCAGA GGATCTACGA GGAGATCCAG AAGATTGAAG CCAATGAGTT TCACTATCAA 
GAGCAGACAG AGCCAATTGA GTTTGTGGCG AACATGAGTG AGAACATGCA GCTGTTCCCG 
AAAGAGCACT TCCTGTGTGG AGACCAGCTG ATGTTTGACT TCAACCCAGA GGTCATTTCA 
GCTGCTTTGT CACTCTTGAC TCCAGGAAAG GCTAATATTT TACTGCTGTC ACCTCAGCAT 
GATGGTCTCT GCCCACTCAA GGAGAAGTGG TTCGGGACAC AGTATAGTGT GGAAGATATT 
CCTCAGGAAT TCCGTGATCT TTGGGCTGGA GATTTTCCTC TGCATCCTGA GCTTCAGCTC 
CCAGCTGAGA ACAAGTTTAT AGCCACGGAT TTCACCCTCA GAACATCAGA TTGTCCTGAT 
ACAGACTTCC CAGTCAAAAT AATAGACAAC GAGAGAGGCC GTCTGTGGTT TAGAAAAGAC 
AACAAGTTCA AGATACCCAA AGCTTATGCC CGCTTCCAGC TGCTCACTCC ATTCATACAG 
GAATCACCTA AAAATTTGGT CCTGTTTGAT CTCTTCGTGA ATATTGTGGC TCATAATCTG 
GCAGAGCTGG CATATGACGC TGAAGTGGCT CAACTACAGT ATAATCTACT GCCAGGAGAT 
CACGGCCTGT TCATCAGACT CAAAGGATTC AACCACAAAC TGCCTTTGCT CCTGAAGCTG 
ATTGTAGATC ACCTGGCCGA CTTCAGCGCC ACTCCAGACG TGTTTAACAT GTTCATTGAG 
CAGCTGAAGA AAACCTACTA CATCATCCTC ATTAGACCAG AACGCCTTGG AAAAGATGTT 
CGGCTGCAGA TTTTGGAGCA CCATCGCTGG TCTGTGATGC AGAAATATGA AGCCATCATG 
GCGGATCCGA GTGTGGCTGA TCTCATGACC TTTGCCAATC GCTTTAAGGC TGAGTTATTT 
GTGGAAGGAC TCGTACAAGG GAACTTCACA AGTGCTGAGT CCAAAGAGTT TCTGCAGTGT 
TTTATTGAGA AACTCAAGTA TGCACCTCAT CCCATTGAAC CTCCTGTGCT GTTTCGAGTG 
GTGGAGTTAC CACAAACACA CCACTTATGT AAAGTCCAGT CGCTCAATAA AGCAGACGCA 
AACTCTGAAG TCACCGTATA CTATCAGACT GGGCTGAAGA ACTTGCGAGA GCATACACTG 
ATGGAGCTGC TTGTTATGCA CATGGAAGAG CCCTGCTTTG ATTTCCTGAG GACAAAGGAA 
ACTCTAGGGT ATCAGGTCTA TCCCATATGC AGAAACACAT CCGGCATTCT TGGCTTCTCC 
GTTACTGTCG AAACTCAGGC CACCAAATTC AGCACGGACT TTGTGGAAGG TAAAATCGAA 
GCATTTCTGG TCAGCTTTGG AGAGAAGTTG GTTCAGCTGT CAGACGAGGC GTTTGGAGCT 
CAAGTGACAG CGCTGATCAA GCTGAAGGAG TGTGAAGACA CACAGCTCGG GGACGAAGTG 
GACAGAAACT GGTTCGAAGT GGTCACTCAG CAGTATGTCT TTGACAGGCT CAATAAAGAA 
ATTGAAATCC TGAAAGACGT CACCAAAGAT GAACTCGTAT CATTTTACAT GGAGCACCGA 
AAAGAAAACA GTAGAAAACT AAGCATACAT GTCGTTGGAT TCGGAGAAGA AGAGAAGGAA 
ATCCATGGAG AAGACCAGTG CAGTTCAAAC CCAGAGACTT CTTCATATGC AAATATTTCC 
AAACTCACTT TCCTCTCAGC GGCGGCCATG AAGGAGAAGT CCACCATGAT AACAGACATC 
CGCGCTTTCA CATCTTCACT CCATCTGCAC CCCTACCACA AAATCCTGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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NRD1B cDNA ORF clone, Danio rerio(Zebrafish)

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Clones

gRNAs

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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