HIPK2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIPK2
Entrez Gene ID	557551
Full Name	homeodomain interacting protein kinase 2
Synonyms	cb1009,si:dkey-16j24.2,si:dkey-239k7.1,wu:fk56d12
General protein information	Preferred Names homeodomain interacting protein kinase 2 Names homeodomain-interacting protein kinase 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005158992.3	XP_005159049.1	homeodomain-interacting protein kinase 2 isoform X2
XM_005158992.4	XP_005159049.1	homeodomain-interacting protein kinase 2 isoform X2
NM_001099985.4	NP_001093455.3	homeodomain-interacting protein kinase 2
XM_005158991.3	XP_005159048.1	homeodomain-interacting protein kinase 2 isoform X1
XM_005158993.4	XP_005159050.1	homeodomain-interacting protein kinase 2 isoform X3
XM_005158991.4	XP_005159048.1	homeodomain-interacting protein kinase 2 isoform X1
XM_005158993.3	XP_005159050.1	homeodomain-interacting protein kinase 2 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HIPK2	XP_001152004.2
Rattus norvegicus (Norway rat)	Hipk2	NP_001102092.1
Homo sapiens (human)	HIPK2	NP_073577.3
Canis lupus familiaris (dog)	HIPK2	NP_001014769.1
Mus musculus (house mouse)	Hipk2	NP_034563.2
Gallus gallus (chicken)	HIPK2	XP_004937904.1
Danio rerio (zebrafish)	hipk2	NP_001093455.2
Xenopus tropicalis (tropical clawed frog)	hipk2	NP_001072750.1
	HIPK2	XP_002803528.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth.
Mayrhofer M, Gourain V, Reischl M, Affaticati P, Jenett A, Joly JS, Benelli M, Demichelis F, Poliani PL, Sieger D, Mione M
Disease models & mechanisms10(1)15-28(2017 01)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Hipk2 and PP1c cooperate to maintain Dvl protein levels required for Wnt signal transduction.
Shimizu N, Ishitani S, Sato A, Shibuya H, Ishitani T
Cell reports8(5)1391-404(2014 Sep)

CtBP2 downregulation during neural crest specification induces expression of Mitf and REST, resulting in melanocyte differentiation and sympathoadrenal lineage suppression.
Liang H, Fekete DM, Andrisani OM
Molecular and cellular biology31(5)955-70(2011 Mar)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hipk2 promotes PP1c-mediated Dvl dephosphorylation via its C-terminal domain and is essential Dvl stability.
Title: Hipk2 and PP1c cooperate to maintain Dvl protein levels required for Wnt signal transduction.

The following HIPK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIPK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23136	NM_001099985.4 Latest version!	Danio rerio homeodomain interacting protein kinase 2 (hipk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa23137	XM_005158991.4 Latest version!	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa23138	XM_005158992.4 Latest version!	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa23139	XM_005158993.4 Latest version!	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa23138	XM_005158992.3	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa23137	XM_005158991.3	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa23139	XM_005158993.3	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIPK2 ( NM_001099985.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001093455.3 Danio rerio homeodomain interacting protein kinase 2 (hipk2), mRNA.

Price & Availability↑

CloneID	ODa23136
Clone ID Related Accession (Same CDS sequence)	NM_001099985.4
Accession Version	NM_001099985.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	homeodomain-interacting protein kinase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX247877.13, CT025920.8 and AL929229.6. On or before Aug 21, 2015 this sequence version replaced XM_005158990.2, NM_001099985.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB830921.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGGCCAC CAACCTGGCT 
CCCTCTTCCA CCAGTGCCAC CCTTTCCCTT ACCAATCCCG ATGTCTCCAT ACTAAATTAC 
CAATCTGCAC TCTACCAGCC TTCTGCGGCC CCCATGGCTG CCGTGGCACC CAGGACGATG 
CCCCTCCAGC CCGGTGCACC GCAACTCTGT GCCGCCCGAC CCGATCCCTT CCAGCAGGCC 
CTCATCGTCT GCCCACCTGG CTTCCAAGGT TTGCAAGCAT CGCCCTCTAA ACACACTGGC 
TACTCTGTGC GGATGGAGAA CGCAGTTCCC ATAGTAACAC AGGCCCCCGG TGCCCAACCT 
CTGCAGATCC AGCCAGGCCT TCTGACACAG CAGGCCTGGC CATCTGGTAC TCAACAGATT 
CTCCTTCCTC CAGCGTGGCA GCAGCTCACA CACACTTCAG TGCAACACGC CACCGTCATC 
CCAGACTCCA TGACCAGCTC ACAGCCTCTG GCTAACTGGA GGAATTCCCA TCCCCATGGC 
AGCCATTACA ATCCCATTAT GCAACAGCCA GCCTTACTGG CAGGTCACGT CACCCTGCCC 
CCTCAGCAAC CTCTTAATGT GGGTGTGGCT CATGTCATGA GACAGCCATC CAACAACCAA 
TCAAACTCCA AAAAGAACAA GCAGCACCAG ATGAACAACA GGAACGTGTC AGGGTACGAC 
GTGTCCTCCA CCCAGCCGGT GACGTCACCT CAGCGCTCCA AACGGGTGAA AGAGAACACC 
CCGCCTCGCT GTGCCGTGGT TCAAAATGGC CCTCCCTCCA CCTGCGTCCC ATCACAGACC 
TGCACAGGCG GTGGATGGGG AGGTGAACAG GCATGTAGCA CTACTCGAGA CCACCACGGT 
CAACACAACC CACCACGCCA AACCATCATC ATCCCCGACA CCCCGAGCCC AGCGGTCAGC 
ATCATCACCA TCAGCAGTGA CACGGATGAG GAGGATGACC TCAAACAACC CACTAACACC 
AGCACATCAT CCAAACAGAG GAAGAATGTC ATCAGCTGTG TGACCGTTCA CGATTCCCCT 
GACTCTGATT CCAGCAATAC CAGCCCTTAC GCTGTGGACT CCCGGACCAA TGGCCCCAAC 
GCCAATGGCT ACGACTCTAA AGGTGCAGTC CTGGATAACT ACAGCAATGG GAACCCCCGC 
ACCATCATCA TCCCCCCGCT GAAGAGCCAG ACCAGCGAAA ATCTGGGAGA GTGTGATAGA 
CTCTTGCCTG CAGATGTCAT GAACCACCAG AACTCCACCT ACAAGTTCAA AACCTCCAAT 
GGAGGCTTCT CTGGCAACAA CCACACATCA GGAGGAGTGA CAGGAGGTGG GGCATACCGG 
CAGCAGCGCA CAGGACCACA CCCCTTTCAG CAGCAGCCTC TAAATCTCAG CCAGGCCCAG 
CAGCACATGG CAGTTGAGCG TAACGGAGGT CACCGGCGGC AGCAGGCATA CATCACCCCC 
ACTATGGCCA CCCAGGCGCA GTACTCCTTC CACAACAGCC CCACCCACAC GGCTAACGTT 
CACCCGCACC TGGCCGCTCC ACATCTGCCT GGGCAACCAC ACCTCTACAC TTACACTGCC 
CCTGCTGCCC TGGGCTCCAC TGGCACAGTG GCCCACCTGG TCGCTTCGCA GGGTTCAGCT 
CGTCACGCAG TCCAGCACGC CAGCTATCCT CCAGGCATTG TCCACCAGGT GCCTGTAAGC 
ATGGGTCACC GCGTGCTGCC CTCGCCCACC CTTCACCATG GCCAATACCA GGCCCAATTT 
GCCCACCAGA CCTACATCAG TGCTTCTCCT GTCTACACTG GATACCCGCT GAGCCCCACC 
AAGATGAACC AGTACCCTTA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093455.3
CDS	338..4024
Translation	MAPVYEGMASQVQVFSPHTLQSSAFFSVKKLKVEQSCNWDMTGYGTHSKVYSQNSKQSVSVAPVGINGASLQVSNSSLSYEQALLFPAGSGHIVVASASSTSGAAGQLLGSTGSSGSGSGGHNLTRRSTVSLLDTYQRCGLKRKSEELDNNNGSGSGSSVHVVEEQQPPAPMIQNNVQSGATVATATASTTATSKNSGANSEGDYQLLQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPARQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPDSTYPLWRLKTPEDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLAEKADRREFIDLLTKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLNTVHSQATNLAPSSTSATLSLTNPDVSILNYQSALYQPSAAPMAAVAPRTMPLQPGAPQLCAARPDPFQQALIVCPPGFQGLQASPSKHTGYSVRMENAVPIVTQAPGAQPLQIQPGLLTQQAWPSGTQQILLPPAWQQLTHTSVQHATVIPDSMTSSQPLANWRNSHPHGSHYNPIMQQPALLAGHVTLPPQQPLNVGVAHVMRQPSNNQSNSKKNKQHQMNNRNVSGYDVSSTQPVTSPQRSKRVKENTPPRCAVVQNGPPSTCVPSQTCTGGGWGGEQACSTTRDHHGQHNPPRQTIIIPDTPSPAVSIITISSDTDEEDDLKQPTNTSTSSKQRKNVISCVTVHDSPDSDSSNTSPYAVDSRTNGPNANGYDSKGAVLDNYSNGNPRTIIIPPLKSQTSENLGECDRLLPADVMNHQNSTYKFKTSNGGFSGNNHTSGGVTGGGAYRQQRTGPHPFQQQPLNLSQAQQHMAVERNGGHRRQQAYITPTMATQAQYSFHNSPTHTANVHPHLAAPHLPGQPHLYTYTAPAALGSTGTVAHLVASQGSARHAVQHASYPPGIVHQVPVSMGHRVLPSPTLHHGQYQAQFAHQTYISASPVYTGYPLSPTKMNQYPYL

Target ORF information:

RefSeq Version	NM_001099985.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio homeodomain interacting protein kinase 2 (hipk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099985.4

ORF Insert Sequence:

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGGCCAC CAACCTGGCT 
CCCTCTTCCA CCAGTGCCAC CCTTTCCCTT ACCAATCCCG ATGTCTCCAT ACTAAATTAC 
CAATCTGCAC TCTACCAGCC TTCTGCGGCC CCCATGGCTG CCGTGGCACC CAGGACGATG 
CCCCTCCAGC CCGGTGCACC GCAACTCTGT GCCGCCCGAC CCGATCCCTT CCAGCAGGCC 
CTCATCGTCT GCCCACCTGG CTTCCAAGGT TTGCAAGCAT CGCCCTCTAA ACACACTGGC 
TACTCTGTGC GGATGGAGAA CGCAGTTCCC ATAGTAACAC AGGCCCCCGG TGCCCAACCT 
CTGCAGATCC AGCCAGGCCT TCTGACACAG CAGGCCTGGC CATCTGGTAC TCAACAGATT 
CTCCTTCCTC CAGCGTGGCA GCAGCTCACA CACACTTCAG TGCAACACGC CACCGTCATC 
CCAGACTCCA TGACCAGCTC ACAGCCTCTG GCTAACTGGA GGAATTCCCA TCCCCATGGC 
AGCCATTACA ATCCCATTAT GCAACAGCCA GCCTTACTGG CAGGTCACGT CACCCTGCCC 
CCTCAGCAAC CTCTTAATGT GGGTGTGGCT CATGTCATGA GACAGCCATC CAACAACCAA 
TCAAACTCCA AAAAGAACAA GCAGCACCAG ATGAACAACA GGAACGTGTC AGGGTACGAC 
GTGTCCTCCA CCCAGCCGGT GACGTCACCT CAGCGCTCCA AACGGGTGAA AGAGAACACC 
CCGCCTCGCT GTGCCGTGGT TCAAAATGGC CCTCCCTCCA CCTGCGTCCC ATCACAGACC 
TGCACAGGCG GTGGATGGGG AGGTGAACAG GCATGTAGCA CTACTCGAGA CCACCACGGT 
CAACACAACC CACCACGCCA AACCATCATC ATCCCCGACA CCCCGAGCCC AGCGGTCAGC 
ATCATCACCA TCAGCAGTGA CACGGATGAG GAGGATGACC TCAAACAACC CACTAACACC 
AGCACATCAT CCAAACAGAG GAAGAATGTC ATCAGCTGTG TGACCGTTCA CGATTCCCCT 
GACTCTGATT CCAGCAATAC CAGCCCTTAC GCTGTGGACT CCCGGACCAA TGGCCCCAAC 
GCCAATGGCT ACGACTCTAA AGGTGCAGTC CTGGATAACT ACAGCAATGG GAACCCCCGC 
ACCATCATCA TCCCCCCGCT GAAGAGCCAG ACCAGCGAAA ATCTGGGAGA GTGTGATAGA 
CTCTTGCCTG CAGATGTCAT GAACCACCAG AACTCCACCT ACAAGTTCAA AACCTCCAAT 
GGAGGCTTCT CTGGCAACAA CCACACATCA GGAGGAGTGA CAGGAGGTGG GGCATACCGG 
CAGCAGCGCA CAGGACCACA CCCCTTTCAG CAGCAGCCTC TAAATCTCAG CCAGGCCCAG 
CAGCACATGG CAGTTGAGCG TAACGGAGGT CACCGGCGGC AGCAGGCATA CATCACCCCC 
ACTATGGCCA CCCAGGCGCA GTACTCCTTC CACAACAGCC CCACCCACAC GGCTAACGTT 
CACCCGCACC TGGCCGCTCC ACATCTGCCT GGGCAACCAC ACCTCTACAC TTACACTGCC 
CCTGCTGCCC TGGGCTCCAC TGGCACAGTG GCCCACCTGG TCGCTTCGCA GGGTTCAGCT 
CGTCACGCAG TCCAGCACGC CAGCTATCCT CCAGGCATTG TCCACCAGGT GCCTGTAAGC 
ATGGGTCACC GCGTGCTGCC CTCGCCCACC CTTCACCATG GCCAATACCA GGCCCAATTT 
GCCCACCAGA CCTACATCAG TGCTTCTCCT GTCTACACTG GATACCCGCT GAGCCCCACC 
AAGATGAACC AGTACCCTTA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIPK2 ( XM_005158991.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159048.1 Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23137
Clone ID Related Accession (Same CDS sequence)	XM_005158991.3 , XM_005158991.4
Accession Version	XM_005158991.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	homeodomain-interacting protein kinase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGGCCAC CAACCTGGCT 
CCCTCTTCCA CCAGTGCCAC CCTTTCCCTT ACCAATCCCG ATGTCTCCAT ACTAAATTAC 
CAATCTGCAC TCTACCAGCC TTCTGCGGCC CCCATGGCTG CCGTGGCACC CAGGACGATG 
CCCCTCCAGC CCGGTGCACC GCAACTCTGT GCCGCCCGAC CCGATCCCTT CCAGCAGGCC 
CTCATCGTCT GCCCACCTGG CTTCCAAGGT TTGCAAGCAT CGCCCTCTAA ACACACTGGC 
TACTCTGTGC GGATGGAGAA CGCAGTTCCC ATAGTAACAC AGGCCCCCGG TGCCCAACCT 
CTGCAGATCC AGCCAGGCCT TCTGACACAG CAGGCCTGGC CATCTGGTAC TCAACAGATT 
CTCCTTCCTC CAGCGTGGCA GCAGCTCACA CACACTTCAG TGCAACACGC CACCGTCATC 
CCAGACTCCA TGACCAGCTC ACAGCCTCTG GCTAACTGGA GGAATTCCCA TCCCCATGGC 
AGCCATTACA ATCCCATTAT GCAACAGCCA GCCTTACTGG CAGGTCACGT CACCCTGCCC 
CCTCAGCAAC CTCTTAATGT GGGTGTGGCT CATGTCATGA GACAGCCATC CAACAACCAA 
TCAAACTCCA AAAAGAACAA GCAGCACCAG ATGAACAACA GGAACGTGTC AGGGTACGAC 
GTGTCCTCCA CCCAGCCGGT GACGTCACCT CAGCGCTCCA AACGGGTGAA AGAGAACACC 
CCGCCTCGCT GTGCCGTGGT TCAAAATGGC CCTCCCTCCA CCTGCGTCCC ATCACAGACC 
TGCACAGGCG GTGGATGGGG AGGTGAACAG GCATGTAGCA CTACTCGAGA CCACCACGGT 
CAACACAACC CACCACGCCA AACCATCATC ATCCCCGACA CCCCGAGCCC AGCGGTCAGC 
ATCATCACCA TCAGCAGTGA CACGGATGAG GAGGATGACC TCAAACAACC CACTAACACC 
AGCACATCAT CCAAACAGAG GAAGAATGTC ATCAGCTGTG TGACCGTTCA CGATTCCCCT 
GACTCTGATT CCAGCAATAC CAGCCCTTAC GCTGTGGACT CCCGGACCAA TGGCCCCAAC 
GCCAATGGCT ACGACTCTAA AGGTGCAGTC CTGGATAACT ACAGCAATGG GAACCCCCGC 
ACCATCATCA TCCCCCCGCT GAAGAGCCAG ACCAGCGAAA ATCTGGGAGA GTGTGATAGA 
CTCTTGCCTG ATGTCATGAA CCACCAGAAC TCCACCTACA AGTTCAAAAC CTCCAATGGA 
GGCTTCTCTG GCAACAACCA CACATCAGGA GGAGTGACAG GAGGTGGGGC ATACCGGCAG 
CAGCGCACAG GACCACACCC CTTTCAGCAG CAGCCTCTAA ATCTCAGCCA GGCCCAGCAG 
CACATGGCAG TTGAGCGTAA CGGAGGTCAC CGGCGGCAGC AGGCATACAT CACCCCCACT 
ATGGCCACCC AGGCGCAGTA CTCCTTCCAC AACAGCCCCA CCCACACGGC TAACGTTCAC 
CCGCACCTGG CCGCTCCACA TCTGCCTGGG CAACCACACC TCTACACTTA CACTGCCCCT 
GCTGCCCTGG GCTCCACTGG CACAGTGGCC CACCTGGTCG CTTCGCAGGG TTCAGCTCGT 
CACGCAGTCC AGCACGCCAG CTATCCTCCA GGCATTGTCC ACCAGGTGCC TGTAAGCATG 
GGTCACCGCG TGCTGCCCTC GCCCACCCTT CACCATGGCC AATACCAGGC CCAATTTGCC 
CACCAGACCT ACATCAGTGC TTCTCCTGTC TACACTGGAT ACCCGCTGAG CCCCACCAAG 
ATGAACCAGT ACCCTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159048.1
CDS	355..4038
Translation	MAPVYEGMASQVQVFSPHTLQSSAFFSVKKLKVEQSCNWDMTGYGTHSKVYSQNSKQSVSVAPVGINGASLQVSNSSLSYEQALLFPAGSGHIVVASASSTSGAAGQLLGSTGSSGSGSGGHNLTRRSTVSLLDTYQRCGLKRKSEELDNNNGSGSGSSVHVVEEQQPPAPMIQNNVQSGATVATATASTTATSKNSGANSEGDYQLLQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPARQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPDSTYPLWRLKTPEDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLAEKADRREFIDLLTKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLNTVHSQATNLAPSSTSATLSLTNPDVSILNYQSALYQPSAAPMAAVAPRTMPLQPGAPQLCAARPDPFQQALIVCPPGFQGLQASPSKHTGYSVRMENAVPIVTQAPGAQPLQIQPGLLTQQAWPSGTQQILLPPAWQQLTHTSVQHATVIPDSMTSSQPLANWRNSHPHGSHYNPIMQQPALLAGHVTLPPQQPLNVGVAHVMRQPSNNQSNSKKNKQHQMNNRNVSGYDVSSTQPVTSPQRSKRVKENTPPRCAVVQNGPPSTCVPSQTCTGGGWGGEQACSTTRDHHGQHNPPRQTIIIPDTPSPAVSIITISSDTDEEDDLKQPTNTSTSSKQRKNVISCVTVHDSPDSDSSNTSPYAVDSRTNGPNANGYDSKGAVLDNYSNGNPRTIIIPPLKSQTSENLGECDRLLPDVMNHQNSTYKFKTSNGGFSGNNHTSGGVTGGGAYRQQRTGPHPFQQQPLNLSQAQQHMAVERNGGHRRQQAYITPTMATQAQYSFHNSPTHTANVHPHLAAPHLPGQPHLYTYTAPAALGSTGTVAHLVASQGSARHAVQHASYPPGIVHQVPVSMGHRVLPSPTLHHGQYQAQFAHQTYISASPVYTGYPLSPTKMNQYPYL

Target ORF information:

RefSeq Version	XM_005158991.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158991.3

ORF Insert Sequence:

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGGCCAC CAACCTGGCT 
CCCTCTTCCA CCAGTGCCAC CCTTTCCCTT ACCAATCCCG ATGTCTCCAT ACTAAATTAC 
CAATCTGCAC TCTACCAGCC TTCTGCGGCC CCCATGGCTG CCGTGGCACC CAGGACGATG 
CCCCTCCAGC CCGGTGCACC GCAACTCTGT GCCGCCCGAC CCGATCCCTT CCAGCAGGCC 
CTCATCGTCT GCCCACCTGG CTTCCAAGGT TTGCAAGCAT CGCCCTCTAA ACACACTGGC 
TACTCTGTGC GGATGGAGAA CGCAGTTCCC ATAGTAACAC AGGCCCCCGG TGCCCAACCT 
CTGCAGATCC AGCCAGGCCT TCTGACACAG CAGGCCTGGC CATCTGGTAC TCAACAGATT 
CTCCTTCCTC CAGCGTGGCA GCAGCTCACA CACACTTCAG TGCAACACGC CACCGTCATC 
CCAGACTCCA TGACCAGCTC ACAGCCTCTG GCTAACTGGA GGAATTCCCA TCCCCATGGC 
AGCCATTACA ATCCCATTAT GCAACAGCCA GCCTTACTGG CAGGTCACGT CACCCTGCCC 
CCTCAGCAAC CTCTTAATGT GGGTGTGGCT CATGTCATGA GACAGCCATC CAACAACCAA 
TCAAACTCCA AAAAGAACAA GCAGCACCAG ATGAACAACA GGAACGTGTC AGGGTACGAC 
GTGTCCTCCA CCCAGCCGGT GACGTCACCT CAGCGCTCCA AACGGGTGAA AGAGAACACC 
CCGCCTCGCT GTGCCGTGGT TCAAAATGGC CCTCCCTCCA CCTGCGTCCC ATCACAGACC 
TGCACAGGCG GTGGATGGGG AGGTGAACAG GCATGTAGCA CTACTCGAGA CCACCACGGT 
CAACACAACC CACCACGCCA AACCATCATC ATCCCCGACA CCCCGAGCCC AGCGGTCAGC 
ATCATCACCA TCAGCAGTGA CACGGATGAG GAGGATGACC TCAAACAACC CACTAACACC 
AGCACATCAT CCAAACAGAG GAAGAATGTC ATCAGCTGTG TGACCGTTCA CGATTCCCCT 
GACTCTGATT CCAGCAATAC CAGCCCTTAC GCTGTGGACT CCCGGACCAA TGGCCCCAAC 
GCCAATGGCT ACGACTCTAA AGGTGCAGTC CTGGATAACT ACAGCAATGG GAACCCCCGC 
ACCATCATCA TCCCCCCGCT GAAGAGCCAG ACCAGCGAAA ATCTGGGAGA GTGTGATAGA 
CTCTTGCCTG ATGTCATGAA CCACCAGAAC TCCACCTACA AGTTCAAAAC CTCCAATGGA 
GGCTTCTCTG GCAACAACCA CACATCAGGA GGAGTGACAG GAGGTGGGGC ATACCGGCAG 
CAGCGCACAG GACCACACCC CTTTCAGCAG CAGCCTCTAA ATCTCAGCCA GGCCCAGCAG 
CACATGGCAG TTGAGCGTAA CGGAGGTCAC CGGCGGCAGC AGGCATACAT CACCCCCACT 
ATGGCCACCC AGGCGCAGTA CTCCTTCCAC AACAGCCCCA CCCACACGGC TAACGTTCAC 
CCGCACCTGG CCGCTCCACA TCTGCCTGGG CAACCACACC TCTACACTTA CACTGCCCCT 
GCTGCCCTGG GCTCCACTGG CACAGTGGCC CACCTGGTCG CTTCGCAGGG TTCAGCTCGT 
CACGCAGTCC AGCACGCCAG CTATCCTCCA GGCATTGTCC ACCAGGTGCC TGTAAGCATG 
GGTCACCGCG TGCTGCCCTC GCCCACCCTT CACCATGGCC AATACCAGGC CCAATTTGCC 
CACCAGACCT ACATCAGTGC TTCTCCTGTC TACACTGGAT ACCCGCTGAG CCCCACCAAG 
ATGAACCAGT ACCCTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIPK2 ( XM_005158991.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159048.1 Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X1, mRNA.

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CloneID	ODa23137
Clone ID Related Accession (Same CDS sequence)	XM_005158991.3 , XM_005158991.4
Accession Version	XM_005158991.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeodomain-interacting protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158991.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGGCCAC CAACCTGGCT 
CCCTCTTCCA CCAGTGCCAC CCTTTCCCTT ACCAATCCCG ATGTCTCCAT ACTAAATTAC 
CAATCTGCAC TCTACCAGCC TTCTGCGGCC CCCATGGCTG CCGTGGCACC CAGGACGATG 
CCCCTCCAGC CCGGTGCACC GCAACTCTGT GCCGCCCGAC CCGATCCCTT CCAGCAGGCC 
CTCATCGTCT GCCCACCTGG CTTCCAAGGT TTGCAAGCAT CGCCCTCTAA ACACACTGGC 
TACTCTGTGC GGATGGAGAA CGCAGTTCCC ATAGTAACAC AGGCCCCCGG TGCCCAACCT 
CTGCAGATCC AGCCAGGCCT TCTGACACAG CAGGCCTGGC CATCTGGTAC TCAACAGATT 
CTCCTTCCTC CAGCGTGGCA GCAGCTCACA CACACTTCAG TGCAACACGC CACCGTCATC 
CCAGACTCCA TGACCAGCTC ACAGCCTCTG GCTAACTGGA GGAATTCCCA TCCCCATGGC 
AGCCATTACA ATCCCATTAT GCAACAGCCA GCCTTACTGG CAGGTCACGT CACCCTGCCC 
CCTCAGCAAC CTCTTAATGT GGGTGTGGCT CATGTCATGA GACAGCCATC CAACAACCAA 
TCAAACTCCA AAAAGAACAA GCAGCACCAG ATGAACAACA GGAACGTGTC AGGGTACGAC 
GTGTCCTCCA CCCAGCCGGT GACGTCACCT CAGCGCTCCA AACGGGTGAA AGAGAACACC 
CCGCCTCGCT GTGCCGTGGT TCAAAATGGC CCTCCCTCCA CCTGCGTCCC ATCACAGACC 
TGCACAGGCG GTGGATGGGG AGGTGAACAG GCATGTAGCA CTACTCGAGA CCACCACGGT 
CAACACAACC CACCACGCCA AACCATCATC ATCCCCGACA CCCCGAGCCC AGCGGTCAGC 
ATCATCACCA TCAGCAGTGA CACGGATGAG GAGGATGACC TCAAACAACC CACTAACACC 
AGCACATCAT CCAAACAGAG GAAGAATGTC ATCAGCTGTG TGACCGTTCA CGATTCCCCT 
GACTCTGATT CCAGCAATAC CAGCCCTTAC GCTGTGGACT CCCGGACCAA TGGCCCCAAC 
GCCAATGGCT ACGACTCTAA AGGTGCAGTC CTGGATAACT ACAGCAATGG GAACCCCCGC 
ACCATCATCA TCCCCCCGCT GAAGAGCCAG ACCAGCGAAA ATCTGGGAGA GTGTGATAGA 
CTCTTGCCTG ATGTCATGAA CCACCAGAAC TCCACCTACA AGTTCAAAAC CTCCAATGGA 
GGCTTCTCTG GCAACAACCA CACATCAGGA GGAGTGACAG GAGGTGGGGC ATACCGGCAG 
CAGCGCACAG GACCACACCC CTTTCAGCAG CAGCCTCTAA ATCTCAGCCA GGCCCAGCAG 
CACATGGCAG TTGAGCGTAA CGGAGGTCAC CGGCGGCAGC AGGCATACAT CACCCCCACT 
ATGGCCACCC AGGCGCAGTA CTCCTTCCAC AACAGCCCCA CCCACACGGC TAACGTTCAC 
CCGCACCTGG CCGCTCCACA TCTGCCTGGG CAACCACACC TCTACACTTA CACTGCCCCT 
GCTGCCCTGG GCTCCACTGG CACAGTGGCC CACCTGGTCG CTTCGCAGGG TTCAGCTCGT 
CACGCAGTCC AGCACGCCAG CTATCCTCCA GGCATTGTCC ACCAGGTGCC TGTAAGCATG 
GGTCACCGCG TGCTGCCCTC GCCCACCCTT CACCATGGCC AATACCAGGC CCAATTTGCC 
CACCAGACCT ACATCAGTGC TTCTCCTGTC TACACTGGAT ACCCGCTGAG CCCCACCAAG 
ATGAACCAGT ACCCTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159048.1
CDS	353..4036
Translation	MAPVYEGMASQVQVFSPHTLQSSAFFSVKKLKVEQSCNWDMTGYGTHSKVYSQNSKQSVSVAPVGINGASLQVSNSSLSYEQALLFPAGSGHIVVASASSTSGAAGQLLGSTGSSGSGSGGHNLTRRSTVSLLDTYQRCGLKRKSEELDNNNGSGSGSSVHVVEEQQPPAPMIQNNVQSGATVATATASTTATSKNSGANSEGDYQLLQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPARQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPDSTYPLWRLKTPEDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLAEKADRREFIDLLTKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLNTVHSQATNLAPSSTSATLSLTNPDVSILNYQSALYQPSAAPMAAVAPRTMPLQPGAPQLCAARPDPFQQALIVCPPGFQGLQASPSKHTGYSVRMENAVPIVTQAPGAQPLQIQPGLLTQQAWPSGTQQILLPPAWQQLTHTSVQHATVIPDSMTSSQPLANWRNSHPHGSHYNPIMQQPALLAGHVTLPPQQPLNVGVAHVMRQPSNNQSNSKKNKQHQMNNRNVSGYDVSSTQPVTSPQRSKRVKENTPPRCAVVQNGPPSTCVPSQTCTGGGWGGEQACSTTRDHHGQHNPPRQTIIIPDTPSPAVSIITISSDTDEEDDLKQPTNTSTSSKQRKNVISCVTVHDSPDSDSSNTSPYAVDSRTNGPNANGYDSKGAVLDNYSNGNPRTIIIPPLKSQTSENLGECDRLLPDVMNHQNSTYKFKTSNGGFSGNNHTSGGVTGGGAYRQQRTGPHPFQQQPLNLSQAQQHMAVERNGGHRRQQAYITPTMATQAQYSFHNSPTHTANVHPHLAAPHLPGQPHLYTYTAPAALGSTGTVAHLVASQGSARHAVQHASYPPGIVHQVPVSMGHRVLPSPTLHHGQYQAQFAHQTYISASPVYTGYPLSPTKMNQYPYL

Target ORF information:

RefSeq Version	XM_005158991.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158991.4

ORF Insert Sequence:

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGGCCAC CAACCTGGCT 
CCCTCTTCCA CCAGTGCCAC CCTTTCCCTT ACCAATCCCG ATGTCTCCAT ACTAAATTAC 
CAATCTGCAC TCTACCAGCC TTCTGCGGCC CCCATGGCTG CCGTGGCACC CAGGACGATG 
CCCCTCCAGC CCGGTGCACC GCAACTCTGT GCCGCCCGAC CCGATCCCTT CCAGCAGGCC 
CTCATCGTCT GCCCACCTGG CTTCCAAGGT TTGCAAGCAT CGCCCTCTAA ACACACTGGC 
TACTCTGTGC GGATGGAGAA CGCAGTTCCC ATAGTAACAC AGGCCCCCGG TGCCCAACCT 
CTGCAGATCC AGCCAGGCCT TCTGACACAG CAGGCCTGGC CATCTGGTAC TCAACAGATT 
CTCCTTCCTC CAGCGTGGCA GCAGCTCACA CACACTTCAG TGCAACACGC CACCGTCATC 
CCAGACTCCA TGACCAGCTC ACAGCCTCTG GCTAACTGGA GGAATTCCCA TCCCCATGGC 
AGCCATTACA ATCCCATTAT GCAACAGCCA GCCTTACTGG CAGGTCACGT CACCCTGCCC 
CCTCAGCAAC CTCTTAATGT GGGTGTGGCT CATGTCATGA GACAGCCATC CAACAACCAA 
TCAAACTCCA AAAAGAACAA GCAGCACCAG ATGAACAACA GGAACGTGTC AGGGTACGAC 
GTGTCCTCCA CCCAGCCGGT GACGTCACCT CAGCGCTCCA AACGGGTGAA AGAGAACACC 
CCGCCTCGCT GTGCCGTGGT TCAAAATGGC CCTCCCTCCA CCTGCGTCCC ATCACAGACC 
TGCACAGGCG GTGGATGGGG AGGTGAACAG GCATGTAGCA CTACTCGAGA CCACCACGGT 
CAACACAACC CACCACGCCA AACCATCATC ATCCCCGACA CCCCGAGCCC AGCGGTCAGC 
ATCATCACCA TCAGCAGTGA CACGGATGAG GAGGATGACC TCAAACAACC CACTAACACC 
AGCACATCAT CCAAACAGAG GAAGAATGTC ATCAGCTGTG TGACCGTTCA CGATTCCCCT 
GACTCTGATT CCAGCAATAC CAGCCCTTAC GCTGTGGACT CCCGGACCAA TGGCCCCAAC 
GCCAATGGCT ACGACTCTAA AGGTGCAGTC CTGGATAACT ACAGCAATGG GAACCCCCGC 
ACCATCATCA TCCCCCCGCT GAAGAGCCAG ACCAGCGAAA ATCTGGGAGA GTGTGATAGA 
CTCTTGCCTG ATGTCATGAA CCACCAGAAC TCCACCTACA AGTTCAAAAC CTCCAATGGA 
GGCTTCTCTG GCAACAACCA CACATCAGGA GGAGTGACAG GAGGTGGGGC ATACCGGCAG 
CAGCGCACAG GACCACACCC CTTTCAGCAG CAGCCTCTAA ATCTCAGCCA GGCCCAGCAG 
CACATGGCAG TTGAGCGTAA CGGAGGTCAC CGGCGGCAGC AGGCATACAT CACCCCCACT 
ATGGCCACCC AGGCGCAGTA CTCCTTCCAC AACAGCCCCA CCCACACGGC TAACGTTCAC 
CCGCACCTGG CCGCTCCACA TCTGCCTGGG CAACCACACC TCTACACTTA CACTGCCCCT 
GCTGCCCTGG GCTCCACTGG CACAGTGGCC CACCTGGTCG CTTCGCAGGG TTCAGCTCGT 
CACGCAGTCC AGCACGCCAG CTATCCTCCA GGCATTGTCC ACCAGGTGCC TGTAAGCATG 
GGTCACCGCG TGCTGCCCTC GCCCACCCTT CACCATGGCC AATACCAGGC CCAATTTGCC 
CACCAGACCT ACATCAGTGC TTCTCCTGTC TACACTGGAT ACCCGCTGAG CCCCACCAAG 
ATGAACCAGT ACCCTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIPK2 ( XM_005158992.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159049.1 Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23138
Clone ID Related Accession (Same CDS sequence)	XM_005158992.3 , XM_005158992.4
Accession Version	XM_005158992.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	homeodomain-interacting protein kinase 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGCCTTC TGCGGCCCCC 
ATGGCTGCCG TGGCACCCAG GACGATGCCC CTCCAGCCCG GTGCACCGCA ACTCTGTGCC 
GCCCGACCCG ATCCCTTCCA GCAGGCCCTC ATCGTCTGCC CACCTGGCTT CCAAGGTTTG 
CAAGCATCGC CCTCTAAACA CACTGGCTAC TCTGTGCGGA TGGAGAACGC AGTTCCCATA 
GTAACACAGG CCCCCGGTGC CCAACCTCTG CAGATCCAGC CAGGCCTTCT GACACAGCAG 
GCCTGGCCAT CTGGTACTCA ACAGATTCTC CTTCCTCCAG CGTGGCAGCA GCTCACACAC 
ACTTCAGTGC AACACGCCAC CGTCATCCCA GACTCCATGA CCAGCTCACA GCCTCTGGCT 
AACTGGAGGA ATTCCCATCC CCATGGCAGC CATTACAATC CCATTATGCA ACAGCCAGCC 
TTACTGGCAG GTCACGTCAC CCTGCCCCCT CAGCAACCTC TTAATGTGGG TGTGGCTCAT 
GTCATGAGAC AGCCATCCAA CAACCAATCA AACTCCAAAA AGAACAAGCA GCACCAGATG 
AACAACAGGA ACGTGTCAGG GTACGACGTG TCCTCCACCC AGCCGGTGAC GTCACCTCAG 
CGCTCCAAAC GGGTGAAAGA GAACACCCCG CCTCGCTGTG CCGTGGTTCA AAATGGCCCT 
CCCTCCACCT GCGTCCCATC ACAGACCTGC ACAGGCGGTG GATGGGGAGG TGAACAGGCA 
TGTAGCACTA CTCGAGACCA CCACGGTCAA CACAACCCAC CACGCCAAAC CATCATCATC 
CCCGACACCC CGAGCCCAGC GGTCAGCATC ATCACCATCA GCAGTGACAC GGATGAGGAG 
GATGACCTCA AACAACCCAC TAACACCAGC ACATCATCCA AACAGAGGAA GAATGTCATC 
AGCTGTGTGA CCGTTCACGA TTCCCCTGAC TCTGATTCCA GCAATACCAG CCCTTACGCT 
GTGGACTCCC GGACCAATGG CCCCAACGCC AATGGCTACG ACTCTAAAGG TGCAGTCCTG 
GATAACTACA GCAATGGGAA CCCCCGCACC ATCATCATCC CCCCGCTGAA GAGCCAGACC 
AGCGAAAATC TGGGAGAGTG TGATAGACTC TTGCCTGCAG ATGTCATGAA CCACCAGAAC 
TCCACCTACA AGTTCAAAAC CTCCAATGGA GGCTTCTCTG GCAACAACCA CACATCAGGA 
GGAGTGACAG GAGGTGGGGC ATACCGGCAG CAGCGCACAG GACCACACCC CTTTCAGCAG 
CAGCCTCTAA ATCTCAGCCA GGCCCAGCAG CACATGGCAG TTGAGCGTAA CGGAGGTCAC 
CGGCGGCAGC AGGCATACAT CACCCCCACT ATGGCCACCC AGGCGCAGTA CTCCTTCCAC 
AACAGCCCCA CCCACACGGC TAACGTTCAC CCGCACCTGG CCGCTCCACA TCTGCCTGGG 
CAACCACACC TCTACACTTA CACTGCCCCT GCTGCCCTGG GCTCCACTGG CACAGTGGCC 
CACCTGGTCG CTTCGCAGGG TTCAGCTCGT CACGCAGTCC AGCACGCCAG CTATCCTCCA 
GGCATTGTCC ACCAGGTGCC TGTAAGCATG GGTCACCGCG TGCTGCCCTC GCCCACCCTT 
CACCATGGCC AATACCAGGC CCAATTTGCC CACCAGACCT ACATCAGTGC TTCTCCTGTC 
TACACTGGAT ACCCGCTGAG CCCCACCAAG ATGAACCAGT ACCCTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159049.1
CDS	355..3948
Translation	MAPVYEGMASQVQVFSPHTLQSSAFFSVKKLKVEQSCNWDMTGYGTHSKVYSQNSKQSVSVAPVGINGASLQVSNSSLSYEQALLFPAGSGHIVVASASSTSGAAGQLLGSTGSSGSGSGGHNLTRRSTVSLLDTYQRCGLKRKSEELDNNNGSGSGSSVHVVEEQQPPAPMIQNNVQSGATVATATASTTATSKNSGANSEGDYQLLQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPARQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPDSTYPLWRLKTPEDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLAEKADRREFIDLLTKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLNTVHSQPSAAPMAAVAPRTMPLQPGAPQLCAARPDPFQQALIVCPPGFQGLQASPSKHTGYSVRMENAVPIVTQAPGAQPLQIQPGLLTQQAWPSGTQQILLPPAWQQLTHTSVQHATVIPDSMTSSQPLANWRNSHPHGSHYNPIMQQPALLAGHVTLPPQQPLNVGVAHVMRQPSNNQSNSKKNKQHQMNNRNVSGYDVSSTQPVTSPQRSKRVKENTPPRCAVVQNGPPSTCVPSQTCTGGGWGGEQACSTTRDHHGQHNPPRQTIIIPDTPSPAVSIITISSDTDEEDDLKQPTNTSTSSKQRKNVISCVTVHDSPDSDSSNTSPYAVDSRTNGPNANGYDSKGAVLDNYSNGNPRTIIIPPLKSQTSENLGECDRLLPADVMNHQNSTYKFKTSNGGFSGNNHTSGGVTGGGAYRQQRTGPHPFQQQPLNLSQAQQHMAVERNGGHRRQQAYITPTMATQAQYSFHNSPTHTANVHPHLAAPHLPGQPHLYTYTAPAALGSTGTVAHLVASQGSARHAVQHASYPPGIVHQVPVSMGHRVLPSPTLHHGQYQAQFAHQTYISASPVYTGYPLSPTKMNQYPYL

Target ORF information:

RefSeq Version	XM_005158992.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158992.3

ORF Insert Sequence:

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGCCTTC TGCGGCCCCC 
ATGGCTGCCG TGGCACCCAG GACGATGCCC CTCCAGCCCG GTGCACCGCA ACTCTGTGCC 
GCCCGACCCG ATCCCTTCCA GCAGGCCCTC ATCGTCTGCC CACCTGGCTT CCAAGGTTTG 
CAAGCATCGC CCTCTAAACA CACTGGCTAC TCTGTGCGGA TGGAGAACGC AGTTCCCATA 
GTAACACAGG CCCCCGGTGC CCAACCTCTG CAGATCCAGC CAGGCCTTCT GACACAGCAG 
GCCTGGCCAT CTGGTACTCA ACAGATTCTC CTTCCTCCAG CGTGGCAGCA GCTCACACAC 
ACTTCAGTGC AACACGCCAC CGTCATCCCA GACTCCATGA CCAGCTCACA GCCTCTGGCT 
AACTGGAGGA ATTCCCATCC CCATGGCAGC CATTACAATC CCATTATGCA ACAGCCAGCC 
TTACTGGCAG GTCACGTCAC CCTGCCCCCT CAGCAACCTC TTAATGTGGG TGTGGCTCAT 
GTCATGAGAC AGCCATCCAA CAACCAATCA AACTCCAAAA AGAACAAGCA GCACCAGATG 
AACAACAGGA ACGTGTCAGG GTACGACGTG TCCTCCACCC AGCCGGTGAC GTCACCTCAG 
CGCTCCAAAC GGGTGAAAGA GAACACCCCG CCTCGCTGTG CCGTGGTTCA AAATGGCCCT 
CCCTCCACCT GCGTCCCATC ACAGACCTGC ACAGGCGGTG GATGGGGAGG TGAACAGGCA 
TGTAGCACTA CTCGAGACCA CCACGGTCAA CACAACCCAC CACGCCAAAC CATCATCATC 
CCCGACACCC CGAGCCCAGC GGTCAGCATC ATCACCATCA GCAGTGACAC GGATGAGGAG 
GATGACCTCA AACAACCCAC TAACACCAGC ACATCATCCA AACAGAGGAA GAATGTCATC 
AGCTGTGTGA CCGTTCACGA TTCCCCTGAC TCTGATTCCA GCAATACCAG CCCTTACGCT 
GTGGACTCCC GGACCAATGG CCCCAACGCC AATGGCTACG ACTCTAAAGG TGCAGTCCTG 
GATAACTACA GCAATGGGAA CCCCCGCACC ATCATCATCC CCCCGCTGAA GAGCCAGACC 
AGCGAAAATC TGGGAGAGTG TGATAGACTC TTGCCTGCAG ATGTCATGAA CCACCAGAAC 
TCCACCTACA AGTTCAAAAC CTCCAATGGA GGCTTCTCTG GCAACAACCA CACATCAGGA 
GGAGTGACAG GAGGTGGGGC ATACCGGCAG CAGCGCACAG GACCACACCC CTTTCAGCAG 
CAGCCTCTAA ATCTCAGCCA GGCCCAGCAG CACATGGCAG TTGAGCGTAA CGGAGGTCAC 
CGGCGGCAGC AGGCATACAT CACCCCCACT ATGGCCACCC AGGCGCAGTA CTCCTTCCAC 
AACAGCCCCA CCCACACGGC TAACGTTCAC CCGCACCTGG CCGCTCCACA TCTGCCTGGG 
CAACCACACC TCTACACTTA CACTGCCCCT GCTGCCCTGG GCTCCACTGG CACAGTGGCC 
CACCTGGTCG CTTCGCAGGG TTCAGCTCGT CACGCAGTCC AGCACGCCAG CTATCCTCCA 
GGCATTGTCC ACCAGGTGCC TGTAAGCATG GGTCACCGCG TGCTGCCCTC GCCCACCCTT 
CACCATGGCC AATACCAGGC CCAATTTGCC CACCAGACCT ACATCAGTGC TTCTCCTGTC 
TACACTGGAT ACCCGCTGAG CCCCACCAAG ATGAACCAGT ACCCTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIPK2 ( XM_005158992.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159049.1 Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23138
Clone ID Related Accession (Same CDS sequence)	XM_005158992.3 , XM_005158992.4
Accession Version	XM_005158992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeodomain-interacting protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158992.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGCCTTC TGCGGCCCCC 
ATGGCTGCCG TGGCACCCAG GACGATGCCC CTCCAGCCCG GTGCACCGCA ACTCTGTGCC 
GCCCGACCCG ATCCCTTCCA GCAGGCCCTC ATCGTCTGCC CACCTGGCTT CCAAGGTTTG 
CAAGCATCGC CCTCTAAACA CACTGGCTAC TCTGTGCGGA TGGAGAACGC AGTTCCCATA 
GTAACACAGG CCCCCGGTGC CCAACCTCTG CAGATCCAGC CAGGCCTTCT GACACAGCAG 
GCCTGGCCAT CTGGTACTCA ACAGATTCTC CTTCCTCCAG CGTGGCAGCA GCTCACACAC 
ACTTCAGTGC AACACGCCAC CGTCATCCCA GACTCCATGA CCAGCTCACA GCCTCTGGCT 
AACTGGAGGA ATTCCCATCC CCATGGCAGC CATTACAATC CCATTATGCA ACAGCCAGCC 
TTACTGGCAG GTCACGTCAC CCTGCCCCCT CAGCAACCTC TTAATGTGGG TGTGGCTCAT 
GTCATGAGAC AGCCATCCAA CAACCAATCA AACTCCAAAA AGAACAAGCA GCACCAGATG 
AACAACAGGA ACGTGTCAGG GTACGACGTG TCCTCCACCC AGCCGGTGAC GTCACCTCAG 
CGCTCCAAAC GGGTGAAAGA GAACACCCCG CCTCGCTGTG CCGTGGTTCA AAATGGCCCT 
CCCTCCACCT GCGTCCCATC ACAGACCTGC ACAGGCGGTG GATGGGGAGG TGAACAGGCA 
TGTAGCACTA CTCGAGACCA CCACGGTCAA CACAACCCAC CACGCCAAAC CATCATCATC 
CCCGACACCC CGAGCCCAGC GGTCAGCATC ATCACCATCA GCAGTGACAC GGATGAGGAG 
GATGACCTCA AACAACCCAC TAACACCAGC ACATCATCCA AACAGAGGAA GAATGTCATC 
AGCTGTGTGA CCGTTCACGA TTCCCCTGAC TCTGATTCCA GCAATACCAG CCCTTACGCT 
GTGGACTCCC GGACCAATGG CCCCAACGCC AATGGCTACG ACTCTAAAGG TGCAGTCCTG 
GATAACTACA GCAATGGGAA CCCCCGCACC ATCATCATCC CCCCGCTGAA GAGCCAGACC 
AGCGAAAATC TGGGAGAGTG TGATAGACTC TTGCCTGCAG ATGTCATGAA CCACCAGAAC 
TCCACCTACA AGTTCAAAAC CTCCAATGGA GGCTTCTCTG GCAACAACCA CACATCAGGA 
GGAGTGACAG GAGGTGGGGC ATACCGGCAG CAGCGCACAG GACCACACCC CTTTCAGCAG 
CAGCCTCTAA ATCTCAGCCA GGCCCAGCAG CACATGGCAG TTGAGCGTAA CGGAGGTCAC 
CGGCGGCAGC AGGCATACAT CACCCCCACT ATGGCCACCC AGGCGCAGTA CTCCTTCCAC 
AACAGCCCCA CCCACACGGC TAACGTTCAC CCGCACCTGG CCGCTCCACA TCTGCCTGGG 
CAACCACACC TCTACACTTA CACTGCCCCT GCTGCCCTGG GCTCCACTGG CACAGTGGCC 
CACCTGGTCG CTTCGCAGGG TTCAGCTCGT CACGCAGTCC AGCACGCCAG CTATCCTCCA 
GGCATTGTCC ACCAGGTGCC TGTAAGCATG GGTCACCGCG TGCTGCCCTC GCCCACCCTT 
CACCATGGCC AATACCAGGC CCAATTTGCC CACCAGACCT ACATCAGTGC TTCTCCTGTC 
TACACTGGAT ACCCGCTGAG CCCCACCAAG ATGAACCAGT ACCCTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159049.1
CDS	352..3945
Translation	MAPVYEGMASQVQVFSPHTLQSSAFFSVKKLKVEQSCNWDMTGYGTHSKVYSQNSKQSVSVAPVGINGASLQVSNSSLSYEQALLFPAGSGHIVVASASSTSGAAGQLLGSTGSSGSGSGGHNLTRRSTVSLLDTYQRCGLKRKSEELDNNNGSGSGSSVHVVEEQQPPAPMIQNNVQSGATVATATASTTATSKNSGANSEGDYQLLQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPARQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPDSTYPLWRLKTPEDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLAEKADRREFIDLLTKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLNTVHSQPSAAPMAAVAPRTMPLQPGAPQLCAARPDPFQQALIVCPPGFQGLQASPSKHTGYSVRMENAVPIVTQAPGAQPLQIQPGLLTQQAWPSGTQQILLPPAWQQLTHTSVQHATVIPDSMTSSQPLANWRNSHPHGSHYNPIMQQPALLAGHVTLPPQQPLNVGVAHVMRQPSNNQSNSKKNKQHQMNNRNVSGYDVSSTQPVTSPQRSKRVKENTPPRCAVVQNGPPSTCVPSQTCTGGGWGGEQACSTTRDHHGQHNPPRQTIIIPDTPSPAVSIITISSDTDEEDDLKQPTNTSTSSKQRKNVISCVTVHDSPDSDSSNTSPYAVDSRTNGPNANGYDSKGAVLDNYSNGNPRTIIIPPLKSQTSENLGECDRLLPADVMNHQNSTYKFKTSNGGFSGNNHTSGGVTGGGAYRQQRTGPHPFQQQPLNLSQAQQHMAVERNGGHRRQQAYITPTMATQAQYSFHNSPTHTANVHPHLAAPHLPGQPHLYTYTAPAALGSTGTVAHLVASQGSARHAVQHASYPPGIVHQVPVSMGHRVLPSPTLHHGQYQAQFAHQTYISASPVYTGYPLSPTKMNQYPYL

Target ORF information:

RefSeq Version	XM_005158992.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158992.4

ORF Insert Sequence:

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGCCTTC TGCGGCCCCC 
ATGGCTGCCG TGGCACCCAG GACGATGCCC CTCCAGCCCG GTGCACCGCA ACTCTGTGCC 
GCCCGACCCG ATCCCTTCCA GCAGGCCCTC ATCGTCTGCC CACCTGGCTT CCAAGGTTTG 
CAAGCATCGC CCTCTAAACA CACTGGCTAC TCTGTGCGGA TGGAGAACGC AGTTCCCATA 
GTAACACAGG CCCCCGGTGC CCAACCTCTG CAGATCCAGC CAGGCCTTCT GACACAGCAG 
GCCTGGCCAT CTGGTACTCA ACAGATTCTC CTTCCTCCAG CGTGGCAGCA GCTCACACAC 
ACTTCAGTGC AACACGCCAC CGTCATCCCA GACTCCATGA CCAGCTCACA GCCTCTGGCT 
AACTGGAGGA ATTCCCATCC CCATGGCAGC CATTACAATC CCATTATGCA ACAGCCAGCC 
TTACTGGCAG GTCACGTCAC CCTGCCCCCT CAGCAACCTC TTAATGTGGG TGTGGCTCAT 
GTCATGAGAC AGCCATCCAA CAACCAATCA AACTCCAAAA AGAACAAGCA GCACCAGATG 
AACAACAGGA ACGTGTCAGG GTACGACGTG TCCTCCACCC AGCCGGTGAC GTCACCTCAG 
CGCTCCAAAC GGGTGAAAGA GAACACCCCG CCTCGCTGTG CCGTGGTTCA AAATGGCCCT 
CCCTCCACCT GCGTCCCATC ACAGACCTGC ACAGGCGGTG GATGGGGAGG TGAACAGGCA 
TGTAGCACTA CTCGAGACCA CCACGGTCAA CACAACCCAC CACGCCAAAC CATCATCATC 
CCCGACACCC CGAGCCCAGC GGTCAGCATC ATCACCATCA GCAGTGACAC GGATGAGGAG 
GATGACCTCA AACAACCCAC TAACACCAGC ACATCATCCA AACAGAGGAA GAATGTCATC 
AGCTGTGTGA CCGTTCACGA TTCCCCTGAC TCTGATTCCA GCAATACCAG CCCTTACGCT 
GTGGACTCCC GGACCAATGG CCCCAACGCC AATGGCTACG ACTCTAAAGG TGCAGTCCTG 
GATAACTACA GCAATGGGAA CCCCCGCACC ATCATCATCC CCCCGCTGAA GAGCCAGACC 
AGCGAAAATC TGGGAGAGTG TGATAGACTC TTGCCTGCAG ATGTCATGAA CCACCAGAAC 
TCCACCTACA AGTTCAAAAC CTCCAATGGA GGCTTCTCTG GCAACAACCA CACATCAGGA 
GGAGTGACAG GAGGTGGGGC ATACCGGCAG CAGCGCACAG GACCACACCC CTTTCAGCAG 
CAGCCTCTAA ATCTCAGCCA GGCCCAGCAG CACATGGCAG TTGAGCGTAA CGGAGGTCAC 
CGGCGGCAGC AGGCATACAT CACCCCCACT ATGGCCACCC AGGCGCAGTA CTCCTTCCAC 
AACAGCCCCA CCCACACGGC TAACGTTCAC CCGCACCTGG CCGCTCCACA TCTGCCTGGG 
CAACCACACC TCTACACTTA CACTGCCCCT GCTGCCCTGG GCTCCACTGG CACAGTGGCC 
CACCTGGTCG CTTCGCAGGG TTCAGCTCGT CACGCAGTCC AGCACGCCAG CTATCCTCCA 
GGCATTGTCC ACCAGGTGCC TGTAAGCATG GGTCACCGCG TGCTGCCCTC GCCCACCCTT 
CACCATGGCC AATACCAGGC CCAATTTGCC CACCAGACCT ACATCAGTGC TTCTCCTGTC 
TACACTGGAT ACCCGCTGAG CCCCACCAAG ATGAACCAGT ACCCTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIPK2 ( XM_005158993.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159050.1 Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X3, mRNA.

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CloneID	ODa23139
Clone ID Related Accession (Same CDS sequence)	XM_005158993.4 , XM_005158993.3
Accession Version	XM_005158993.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeodomain-interacting protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158993.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGCCTTC TGCGGCCCCC 
ATGGCTGCCG TGGCACCCAG GACGATGCCC CTCCAGCCCG GTGCACCGCA ACTCTGTGCC 
GCCCGACCCG ATCCCTTCCA GCAGGCCCTC ATCGTCTGCC CACCTGGCTT CCAAGGTTTG 
CAAGCATCGC CCTCTAAACA CACTGGCTAC TCTGTGCGGA TGGAGAACGC AGTTCCCATA 
GTAACACAGG CCCCCGGTGC CCAACCTCTG CAGATCCAGC CAGGCCTTCT GACACAGCAG 
GCCTGGCCAT CTGGTACTCA ACAGATTCTC CTTCCTCCAG CGTGGCAGCA GCTCACACAC 
ACTTCAGTGC AACACGCCAC CGTCATCCCA GACTCCATGA CCAGCTCACA GCCTCTGGCT 
AACTGGAGGA ATTCCCATCC CCATGGCAGC CATTACAATC CCATTATGCA ACAGCCAGCC 
TTACTGGCAG GTCACGTCAC CCTGCCCCCT CAGCAACCTC TTAATGTGGG TGTGGCTCAT 
GTCATGAGAC AGCCATCCAA CAACCAATCA AACTCCAAAA AGAACAAGCA GCACCAGATG 
AACAACAGGA ACGTGTCAGG GTACGACGTG TCCTCCACCC AGCCGGTGAC GTCACCTCAG 
CGCTCCAAAC GGGTGAAAGA GAACACCCCG CCTCGCTGTG CCGTGGTTCA AAATGGCCCT 
CCCTCCACCT GCGTCCCATC ACAGACCTGC ACAGGCGGTG GATGGGGAGG TGAACAGGCA 
TGTAGCACTA CTCGAGACCA CCACGGTCAA CACAACCCAC CACGCCAAAC CATCATCATC 
CCCGACACCC CGAGCCCAGC GGTCAGCATC ATCACCATCA GCAGTGACAC GGATGAGGAG 
GATGACCTCA AACAACCCAC TAACACCAGC ACATCATCCA AACAGAGGAA GAATGTCATC 
AGCTGTGTGA CCGTTCACGA TTCCCCTGAC TCTGATTCCA GCAATACCAG CCCTTACGCT 
GTGGACTCCC GGACCAATGG CCCCAACGCC AATGGCTACG ACTCTAAAGG TGCAGTCCTG 
GATAACTACA GCAATGGGAA CCCCCGCACC ATCATCATCC CCCCGCTGAA GAGCCAGACC 
AGCGAAAATC TGGGAGAGTG TGATAGACTC TTGCCTGATG TCATGAACCA CCAGAACTCC 
ACCTACAAGT TCAAAACCTC CAATGGAGGC TTCTCTGGCA ACAACCACAC ATCAGGAGGA 
GTGACAGGAG GTGGGGCATA CCGGCAGCAG CGCACAGGAC CACACCCCTT TCAGCAGCAG 
CCTCTAAATC TCAGCCAGGC CCAGCAGCAC ATGGCAGTTG AGCGTAACGG AGGTCACCGG 
CGGCAGCAGG CATACATCAC CCCCACTATG GCCACCCAGG CGCAGTACTC CTTCCACAAC 
AGCCCCACCC ACACGGCTAA CGTTCACCCG CACCTGGCCG CTCCACATCT GCCTGGGCAA 
CCACACCTCT ACACTTACAC TGCCCCTGCT GCCCTGGGCT CCACTGGCAC AGTGGCCCAC 
CTGGTCGCTT CGCAGGGTTC AGCTCGTCAC GCAGTCCAGC ACGCCAGCTA TCCTCCAGGC 
ATTGTCCACC AGGTGCCTGT AAGCATGGGT CACCGCGTGC TGCCCTCGCC CACCCTTCAC 
CATGGCCAAT ACCAGGCCCA ATTTGCCCAC CAGACCTACA TCAGTGCTTC TCCTGTCTAC 
ACTGGATACC CGCTGAGCCC CACCAAGATG AACCAGTACC CTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159050.1
CDS	352..3942
Translation	MAPVYEGMASQVQVFSPHTLQSSAFFSVKKLKVEQSCNWDMTGYGTHSKVYSQNSKQSVSVAPVGINGASLQVSNSSLSYEQALLFPAGSGHIVVASASSTSGAAGQLLGSTGSSGSGSGGHNLTRRSTVSLLDTYQRCGLKRKSEELDNNNGSGSGSSVHVVEEQQPPAPMIQNNVQSGATVATATASTTATSKNSGANSEGDYQLLQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPARQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPDSTYPLWRLKTPEDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLAEKADRREFIDLLTKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLNTVHSQPSAAPMAAVAPRTMPLQPGAPQLCAARPDPFQQALIVCPPGFQGLQASPSKHTGYSVRMENAVPIVTQAPGAQPLQIQPGLLTQQAWPSGTQQILLPPAWQQLTHTSVQHATVIPDSMTSSQPLANWRNSHPHGSHYNPIMQQPALLAGHVTLPPQQPLNVGVAHVMRQPSNNQSNSKKNKQHQMNNRNVSGYDVSSTQPVTSPQRSKRVKENTPPRCAVVQNGPPSTCVPSQTCTGGGWGGEQACSTTRDHHGQHNPPRQTIIIPDTPSPAVSIITISSDTDEEDDLKQPTNTSTSSKQRKNVISCVTVHDSPDSDSSNTSPYAVDSRTNGPNANGYDSKGAVLDNYSNGNPRTIIIPPLKSQTSENLGECDRLLPDVMNHQNSTYKFKTSNGGFSGNNHTSGGVTGGGAYRQQRTGPHPFQQQPLNLSQAQQHMAVERNGGHRRQQAYITPTMATQAQYSFHNSPTHTANVHPHLAAPHLPGQPHLYTYTAPAALGSTGTVAHLVASQGSARHAVQHASYPPGIVHQVPVSMGHRVLPSPTLHHGQYQAQFAHQTYISASPVYTGYPLSPTKMNQYPYL

Target ORF information:

RefSeq Version	XM_005158993.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158993.4

ORF Insert Sequence:

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGCCTTC TGCGGCCCCC 
ATGGCTGCCG TGGCACCCAG GACGATGCCC CTCCAGCCCG GTGCACCGCA ACTCTGTGCC 
GCCCGACCCG ATCCCTTCCA GCAGGCCCTC ATCGTCTGCC CACCTGGCTT CCAAGGTTTG 
CAAGCATCGC CCTCTAAACA CACTGGCTAC TCTGTGCGGA TGGAGAACGC AGTTCCCATA 
GTAACACAGG CCCCCGGTGC CCAACCTCTG CAGATCCAGC CAGGCCTTCT GACACAGCAG 
GCCTGGCCAT CTGGTACTCA ACAGATTCTC CTTCCTCCAG CGTGGCAGCA GCTCACACAC 
ACTTCAGTGC AACACGCCAC CGTCATCCCA GACTCCATGA CCAGCTCACA GCCTCTGGCT 
AACTGGAGGA ATTCCCATCC CCATGGCAGC CATTACAATC CCATTATGCA ACAGCCAGCC 
TTACTGGCAG GTCACGTCAC CCTGCCCCCT CAGCAACCTC TTAATGTGGG TGTGGCTCAT 
GTCATGAGAC AGCCATCCAA CAACCAATCA AACTCCAAAA AGAACAAGCA GCACCAGATG 
AACAACAGGA ACGTGTCAGG GTACGACGTG TCCTCCACCC AGCCGGTGAC GTCACCTCAG 
CGCTCCAAAC GGGTGAAAGA GAACACCCCG CCTCGCTGTG CCGTGGTTCA AAATGGCCCT 
CCCTCCACCT GCGTCCCATC ACAGACCTGC ACAGGCGGTG GATGGGGAGG TGAACAGGCA 
TGTAGCACTA CTCGAGACCA CCACGGTCAA CACAACCCAC CACGCCAAAC CATCATCATC 
CCCGACACCC CGAGCCCAGC GGTCAGCATC ATCACCATCA GCAGTGACAC GGATGAGGAG 
GATGACCTCA AACAACCCAC TAACACCAGC ACATCATCCA AACAGAGGAA GAATGTCATC 
AGCTGTGTGA CCGTTCACGA TTCCCCTGAC TCTGATTCCA GCAATACCAG CCCTTACGCT 
GTGGACTCCC GGACCAATGG CCCCAACGCC AATGGCTACG ACTCTAAAGG TGCAGTCCTG 
GATAACTACA GCAATGGGAA CCCCCGCACC ATCATCATCC CCCCGCTGAA GAGCCAGACC 
AGCGAAAATC TGGGAGAGTG TGATAGACTC TTGCCTGATG TCATGAACCA CCAGAACTCC 
ACCTACAAGT TCAAAACCTC CAATGGAGGC TTCTCTGGCA ACAACCACAC ATCAGGAGGA 
GTGACAGGAG GTGGGGCATA CCGGCAGCAG CGCACAGGAC CACACCCCTT TCAGCAGCAG 
CCTCTAAATC TCAGCCAGGC CCAGCAGCAC ATGGCAGTTG AGCGTAACGG AGGTCACCGG 
CGGCAGCAGG CATACATCAC CCCCACTATG GCCACCCAGG CGCAGTACTC CTTCCACAAC 
AGCCCCACCC ACACGGCTAA CGTTCACCCG CACCTGGCCG CTCCACATCT GCCTGGGCAA 
CCACACCTCT ACACTTACAC TGCCCCTGCT GCCCTGGGCT CCACTGGCAC AGTGGCCCAC 
CTGGTCGCTT CGCAGGGTTC AGCTCGTCAC GCAGTCCAGC ACGCCAGCTA TCCTCCAGGC 
ATTGTCCACC AGGTGCCTGT AAGCATGGGT CACCGCGTGC TGCCCTCGCC CACCCTTCAC 
CATGGCCAAT ACCAGGCCCA ATTTGCCCAC CAGACCTACA TCAGTGCTTC TCCTGTCTAC 
ACTGGATACC CGCTGAGCCC CACCAAGATG AACCAGTACC CTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIPK2 ( XM_005158993.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159050.1 Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X3, mRNA.

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CloneID	ODa23139
Clone ID Related Accession (Same CDS sequence)	XM_005158993.4 , XM_005158993.3
Accession Version	XM_005158993.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	homeodomain-interacting protein kinase 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGCCTTC TGCGGCCCCC 
ATGGCTGCCG TGGCACCCAG GACGATGCCC CTCCAGCCCG GTGCACCGCA ACTCTGTGCC 
GCCCGACCCG ATCCCTTCCA GCAGGCCCTC ATCGTCTGCC CACCTGGCTT CCAAGGTTTG 
CAAGCATCGC CCTCTAAACA CACTGGCTAC TCTGTGCGGA TGGAGAACGC AGTTCCCATA 
GTAACACAGG CCCCCGGTGC CCAACCTCTG CAGATCCAGC CAGGCCTTCT GACACAGCAG 
GCCTGGCCAT CTGGTACTCA ACAGATTCTC CTTCCTCCAG CGTGGCAGCA GCTCACACAC 
ACTTCAGTGC AACACGCCAC CGTCATCCCA GACTCCATGA CCAGCTCACA GCCTCTGGCT 
AACTGGAGGA ATTCCCATCC CCATGGCAGC CATTACAATC CCATTATGCA ACAGCCAGCC 
TTACTGGCAG GTCACGTCAC CCTGCCCCCT CAGCAACCTC TTAATGTGGG TGTGGCTCAT 
GTCATGAGAC AGCCATCCAA CAACCAATCA AACTCCAAAA AGAACAAGCA GCACCAGATG 
AACAACAGGA ACGTGTCAGG GTACGACGTG TCCTCCACCC AGCCGGTGAC GTCACCTCAG 
CGCTCCAAAC GGGTGAAAGA GAACACCCCG CCTCGCTGTG CCGTGGTTCA AAATGGCCCT 
CCCTCCACCT GCGTCCCATC ACAGACCTGC ACAGGCGGTG GATGGGGAGG TGAACAGGCA 
TGTAGCACTA CTCGAGACCA CCACGGTCAA CACAACCCAC CACGCCAAAC CATCATCATC 
CCCGACACCC CGAGCCCAGC GGTCAGCATC ATCACCATCA GCAGTGACAC GGATGAGGAG 
GATGACCTCA AACAACCCAC TAACACCAGC ACATCATCCA AACAGAGGAA GAATGTCATC 
AGCTGTGTGA CCGTTCACGA TTCCCCTGAC TCTGATTCCA GCAATACCAG CCCTTACGCT 
GTGGACTCCC GGACCAATGG CCCCAACGCC AATGGCTACG ACTCTAAAGG TGCAGTCCTG 
GATAACTACA GCAATGGGAA CCCCCGCACC ATCATCATCC CCCCGCTGAA GAGCCAGACC 
AGCGAAAATC TGGGAGAGTG TGATAGACTC TTGCCTGATG TCATGAACCA CCAGAACTCC 
ACCTACAAGT TCAAAACCTC CAATGGAGGC TTCTCTGGCA ACAACCACAC ATCAGGAGGA 
GTGACAGGAG GTGGGGCATA CCGGCAGCAG CGCACAGGAC CACACCCCTT TCAGCAGCAG 
CCTCTAAATC TCAGCCAGGC CCAGCAGCAC ATGGCAGTTG AGCGTAACGG AGGTCACCGG 
CGGCAGCAGG CATACATCAC CCCCACTATG GCCACCCAGG CGCAGTACTC CTTCCACAAC 
AGCCCCACCC ACACGGCTAA CGTTCACCCG CACCTGGCCG CTCCACATCT GCCTGGGCAA 
CCACACCTCT ACACTTACAC TGCCCCTGCT GCCCTGGGCT CCACTGGCAC AGTGGCCCAC 
CTGGTCGCTT CGCAGGGTTC AGCTCGTCAC GCAGTCCAGC ACGCCAGCTA TCCTCCAGGC 
ATTGTCCACC AGGTGCCTGT AAGCATGGGT CACCGCGTGC TGCCCTCGCC CACCCTTCAC 
CATGGCCAAT ACCAGGCCCA ATTTGCCCAC CAGACCTACA TCAGTGCTTC TCCTGTCTAC 
ACTGGATACC CGCTGAGCCC CACCAAGATG AACCAGTACC CTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159050.1
CDS	355..3945
Translation	MAPVYEGMASQVQVFSPHTLQSSAFFSVKKLKVEQSCNWDMTGYGTHSKVYSQNSKQSVSVAPVGINGASLQVSNSSLSYEQALLFPAGSGHIVVASASSTSGAAGQLLGSTGSSGSGSGGHNLTRRSTVSLLDTYQRCGLKRKSEELDNNNGSGSGSSVHVVEEQQPPAPMIQNNVQSGATVATATASTTATSKNSGANSEGDYQLLQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPARQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPDSTYPLWRLKTPEDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLAEKADRREFIDLLTKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLNTVHSQPSAAPMAAVAPRTMPLQPGAPQLCAARPDPFQQALIVCPPGFQGLQASPSKHTGYSVRMENAVPIVTQAPGAQPLQIQPGLLTQQAWPSGTQQILLPPAWQQLTHTSVQHATVIPDSMTSSQPLANWRNSHPHGSHYNPIMQQPALLAGHVTLPPQQPLNVGVAHVMRQPSNNQSNSKKNKQHQMNNRNVSGYDVSSTQPVTSPQRSKRVKENTPPRCAVVQNGPPSTCVPSQTCTGGGWGGEQACSTTRDHHGQHNPPRQTIIIPDTPSPAVSIITISSDTDEEDDLKQPTNTSTSSKQRKNVISCVTVHDSPDSDSSNTSPYAVDSRTNGPNANGYDSKGAVLDNYSNGNPRTIIIPPLKSQTSENLGECDRLLPDVMNHQNSTYKFKTSNGGFSGNNHTSGGVTGGGAYRQQRTGPHPFQQQPLNLSQAQQHMAVERNGGHRRQQAYITPTMATQAQYSFHNSPTHTANVHPHLAAPHLPGQPHLYTYTAPAALGSTGTVAHLVASQGSARHAVQHASYPPGIVHQVPVSMGHRVLPSPTLHHGQYQAQFAHQTYISASPVYTGYPLSPTKMNQYPYL

Target ORF information:

RefSeq Version	XM_005158993.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio homeodomain interacting protein kinase 2 (hipk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158993.3

ORF Insert Sequence:

ATGGCCCCTG TATACGAAGG TATGGCCTCG CAAGTGCAAG TCTTCTCCCC TCACACTCTC 
CAGTCAAGTG CCTTCTTTAG CGTGAAGAAA CTGAAGGTGG AGCAGAGTTG CAACTGGGAT 
ATGACAGGGT ACGGCACGCA CAGCAAGGTC TACAGTCAGA ACAGCAAGCA GAGTGTGTCA 
GTCGCCCCTG TGGGCATCAA CGGCGCCAGT CTCCAGGTTT CCAACTCTTC CCTGTCATAC 
GAACAGGCCC TACTCTTCCC GGCCGGCTCG GGGCACATTG TTGTGGCCTC AGCCAGCAGC 
ACGTCTGGGG CAGCTGGTCA ACTGTTGGGC AGCACTGGTA GCAGCGGAAG CGGCAGCGGA 
GGCCATAACC TGACGCGCCG CAGCACCGTC AGTCTGCTGG ACACCTACCA GCGATGCGGG 
CTTAAGCGCA AAAGTGAGGA ACTTGACAAC AACAACGGCA GTGGTAGTGG GAGTAGTGTG 
CATGTGGTTG AGGAGCAGCA GCCGCCGGCA CCCATGATCC AGAACAACGT GCAGAGCGGG 
GCCACCGTCG CGACCGCGAC TGCCTCCACC ACGGCCACAT CCAAGAACAG TGGAGCCAAC 
AGCGAGGGCG ACTATCAGCT CCTACAACAT GAAGTGCTGT GCTCCATGAC CAACACCTAC 
GAGGTGCTGG AGTTCCTGGG TCGAGGGACG TTCGGGCAAG TGGTGAAGTG CTGGAAACGG 
GGCACCAGTG AGATCGTGGC CATCAAGATC CTGAAGAACC ACCCTTCCTA TGCGAGGCAG 
GGCCAGATTG AGGTGAGCAT ACTGGCACGG CTGAGCACGG AGAGTGCGGA CGACTACAAC 
TTTGTTCGAG CGTACGAGTG CTTCCAGCAC AAGAACCACA CGTGCCTGGT CTTCGAGATG 
TTGGAGCAGA ACCTCTACGA CTTCCTCAAA CAGAACAAGT TCAGCCCGTT GCCGCTCAAG 
TACATCCGAC CGATCCTGCA GCAGGTCGCC ACTGCCCTGA TGAAGCTCAA GAGCCTAGGA 
CTGATCCACG CTGACTTGAA GCCTGAGAAC ATCATGCTTG TAGACCCAGC CCGCCAACCC 
TACAGGGTCA AGGTGATCGA CTTCGGATCT GCTAGTCACG TCTCCAAAGC GGTGTGCTCC 
ACCTACCTGC AATCTAGATA CTATAGGGCT CCAGAGATCA TCCTGGGTCT GCCGTTCTGC 
GAGGCCATAG ACATGTGGTC TTTGGGTTGT GTGATCGCTG AACTGTTTTT GGGATGGCCT 
CTTTATCCCG GAGCATCAGA GTATGACCAG ATCCGCTACA TCTCTCAAAC GCAGGGCTTG 
CCAGCAGAGT ATCTGTTAAG TGCAGGGACG AAAACAACCA GGTTTTTCAA CAGAGACCCC 
GACTCCACCT ATCCTCTATG GAGACTGAAG ACCCCCGAGG ACCACGAGGC AGAGACGGGC 
ATCAAATCTA AAGAGGCTCG CAAATACATC TTCAACTGCC TGGATGATAT GGCGCAGGTG 
AATATGACCA CAGACCTGGA GGGCAGTGAT ATGTTGGCAG AAAAGGCAGA CAGAAGAGAG 
TTCATTGACC TGCTGACCAA AATGCTGACC ATCGACGCAG ACAAGAGAAT AACACCCATC 
GAGACCCTCA ACCACCCCTT CGTCACCATG ACCCACTTGC TGGACTTCCC TCACAGCACA 
CATGTCAAAT CCTGCTTCCA GAACATGGAG ATTTGCAAGC GTCGGGTGAA CATGTACGAT 
ACAGTCAATC AGAGCAAGAC CCCCTTTATA ACACATGTAG CCCCCAGCAC CTCGACCAAT 
CTCACCATGA CCTTCAACAA CCAGCTGAAC ACTGTGCATA GCCAGCCTTC TGCGGCCCCC 
ATGGCTGCCG TGGCACCCAG GACGATGCCC CTCCAGCCCG GTGCACCGCA ACTCTGTGCC 
GCCCGACCCG ATCCCTTCCA GCAGGCCCTC ATCGTCTGCC CACCTGGCTT CCAAGGTTTG 
CAAGCATCGC CCTCTAAACA CACTGGCTAC TCTGTGCGGA TGGAGAACGC AGTTCCCATA 
GTAACACAGG CCCCCGGTGC CCAACCTCTG CAGATCCAGC CAGGCCTTCT GACACAGCAG 
GCCTGGCCAT CTGGTACTCA ACAGATTCTC CTTCCTCCAG CGTGGCAGCA GCTCACACAC 
ACTTCAGTGC AACACGCCAC CGTCATCCCA GACTCCATGA CCAGCTCACA GCCTCTGGCT 
AACTGGAGGA ATTCCCATCC CCATGGCAGC CATTACAATC CCATTATGCA ACAGCCAGCC 
TTACTGGCAG GTCACGTCAC CCTGCCCCCT CAGCAACCTC TTAATGTGGG TGTGGCTCAT 
GTCATGAGAC AGCCATCCAA CAACCAATCA AACTCCAAAA AGAACAAGCA GCACCAGATG 
AACAACAGGA ACGTGTCAGG GTACGACGTG TCCTCCACCC AGCCGGTGAC GTCACCTCAG 
CGCTCCAAAC GGGTGAAAGA GAACACCCCG CCTCGCTGTG CCGTGGTTCA AAATGGCCCT 
CCCTCCACCT GCGTCCCATC ACAGACCTGC ACAGGCGGTG GATGGGGAGG TGAACAGGCA 
TGTAGCACTA CTCGAGACCA CCACGGTCAA CACAACCCAC CACGCCAAAC CATCATCATC 
CCCGACACCC CGAGCCCAGC GGTCAGCATC ATCACCATCA GCAGTGACAC GGATGAGGAG 
GATGACCTCA AACAACCCAC TAACACCAGC ACATCATCCA AACAGAGGAA GAATGTCATC 
AGCTGTGTGA CCGTTCACGA TTCCCCTGAC TCTGATTCCA GCAATACCAG CCCTTACGCT 
GTGGACTCCC GGACCAATGG CCCCAACGCC AATGGCTACG ACTCTAAAGG TGCAGTCCTG 
GATAACTACA GCAATGGGAA CCCCCGCACC ATCATCATCC CCCCGCTGAA GAGCCAGACC 
AGCGAAAATC TGGGAGAGTG TGATAGACTC TTGCCTGATG TCATGAACCA CCAGAACTCC 
ACCTACAAGT TCAAAACCTC CAATGGAGGC TTCTCTGGCA ACAACCACAC ATCAGGAGGA 
GTGACAGGAG GTGGGGCATA CCGGCAGCAG CGCACAGGAC CACACCCCTT TCAGCAGCAG 
CCTCTAAATC TCAGCCAGGC CCAGCAGCAC ATGGCAGTTG AGCGTAACGG AGGTCACCGG 
CGGCAGCAGG CATACATCAC CCCCACTATG GCCACCCAGG CGCAGTACTC CTTCCACAAC 
AGCCCCACCC ACACGGCTAA CGTTCACCCG CACCTGGCCG CTCCACATCT GCCTGGGCAA 
CCACACCTCT ACACTTACAC TGCCCCTGCT GCCCTGGGCT CCACTGGCAC AGTGGCCCAC 
CTGGTCGCTT CGCAGGGTTC AGCTCGTCAC GCAGTCCAGC ACGCCAGCTA TCCTCCAGGC 
ATTGTCCACC AGGTGCCTGT AAGCATGGGT CACCGCGTGC TGCCCTCGCC CACCCTTCAC 
CATGGCCAAT ACCAGGCCCA ATTTGCCCAC CAGACCTACA TCAGTGCTTC TCCTGTCTAC 
ACTGGATACC CGCTGAGCCC CACCAAGATG AACCAGTACC CTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth.
Disease models & mechanisms10(1)15-28(2017 01)
Mayrhofer M,Gourain V,Reischl M,Affaticati P,Jenett A,Joly JS,Benelli M,Demichelis F,Poliani PL,Sieger D,Mione M

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Hipk2 and PP1c cooperate to maintain Dvl protein levels required for Wnt signal transduction.
Cell reports8(5)1391-404(2014 Sep)
Shimizu N,Ishitani S,Sato A,Shibuya H,Ishitani T

CtBP2 downregulation during neural crest specification induces expression of Mitf and REST, resulting in melanocyte differentiation and sympathoadrenal lineage suppression.
Molecular and cellular biology31(5)955-70(2011 Mar)
Liang H,Fekete DM,Andrisani OM

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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HIPK2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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