FREM1B cDNA ORF clone, Danio rerio(Zebrafish)

The following FREM1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa33803 NM_001137658.1
Latest version!
Danio rerio Fras1 related extracellular matrix 1b (frem1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa42552 XM_009296245.3
Latest version!
Danio rerio Fras1 related extracellular matrix 1b (frem1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
ODa42552 XM_009296245.2 Danio rerio Fras1 related extracellular matrix 1b (frem1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa33803
    Clone ID Related Accession (Same CDS sequence) NM_001137658.1
    Accession Version NM_001137658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product Fras-related extracellular matrix protein 1b precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK006470.1. On Nov 14, 2008 this sequence version replaced XM_680234.3. ##Evidence-Data-START## Transcript exon combination :: BK006470.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505376, SAMEA3505377 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGGGGTTT TCTTCCTCGC TCTAACAGTG CTCACCTCGA GTCACCTGTG GTCACATGTC 
    CAAGCTTCGA GTCTAGTGCA GTTAAATCGA GTTATTCGAG TAGCCAGAGG ACAATCTGTG
    TTCATCACAG CCAACGAACT TCAGTTCCAC ATGGATCACA AATCCGAGGC CTGTAAAGTA
    GAAGTGGTGC TTAATGAACC CATCACACAA AGAGTGGGAA AACTCACTCC ACAGGTTTTC
    GACTGCCAGT TCTTTCCAGA TGAGGTGAAG TATGTCCACA ATGGCAGTCC ACTTCTTGAG
    GAAGACACCG TCATGTTGAG AGTCTACAGG TTTACAGACA CCGAGACTTT TGTTGAGTCT
    ATGTTGCTGA AGGTACGTGT GTTTGAGCCT CAGAGAAGCC TTGTAGAGCT TGGAAATGTC
    CCTCTGGTGG TTCCTGAGTT CTATGGCCTT TCCAATGCCA TCAACGCCAG CGTTCTCACC
    TTTAAAACCC AGCCAGATGT GATCTGCACG CTTCGTCTTC TCAGCTCTGA GATCAGTGTT
    CCAGCACTGG GTCAGCTGGT GGTTGAGGAG TCTGGAACAA TGGTGGACAC TGAGGCTGGA
    CCAAGAAAAG TGTTTTTTTG TCAGTTTTTT TTTGGACGTC ACACTGCAAT CGCCTGCCCT
    GGAAATAAAG CCTGTGACCT CAAGACAAGG CAAGTGGAGT TCCTGAAGAC CGACTGCAGT
    GAATTCCTCT CTTCAGGTCT GAAGTACCAG CATCTCAGTC CTCCTTCACC TGAGATTGAC
    TACATCCCAG TCCGGCTGGA GTTTCGAGAC CAGACCTCCC GAGCCCTGCT AGAGACCGAG
    AGTGTCTGGT TACCGGTATT GATCCAGGGG GCGATGCAGA ACCAGCCGCC TCACGCTGCC
    TTCATGTCCA CCTTCATCCT GGAGGTAGAT CAGTTCATCC TGACGCCTCT GAGCACCGCT
    GCGCTGGATG CCAAAGACGA CGAGACACCG CAGGACCAGC TCATTTTCAG TGTCACCAAA
    CCTCCAGCCG AAGGATATAT AACTCATTTA GATGATCACA CTAAAATGGC CTCCTCCTTC
    TCATGGCAAG ACCTGAATGA GATGAAAATC GCATACCAGC CCCCAAACAG CAGTCATACA
    GCCAGGAGGA ACTATGAGGT TGAATTTCAG GCCATTGATG GCTCATTTGC CCCGAGTCTT
    CCTATCCTGA TGCACATCTC CATACGCACT GCAGAGACAA ACGCACCTCG AGTCTCCTGG
    AATATGGGCT TGGATCTGCT GGAAGGCCAA TCCAGACCAA TCACATGGGA GCATCTACAG
    ATTGTAGATA AGGACAATAT TGACGCCGTC ACCTTGATCG CAATGGACGG TCCACTTAAT
    GGACACTTAA GTGTTAGAGG TGGAAAAGGC TTCATGTTTA AGGTCAAGGA TCTCCGAGAG
    GGCGTTGTGG TCTATCATCA CTCGGACAGC GACACCACCA GGGATTACGT CGTCTTCCGA
    ATCACAGATG GCCGCCACAG CATCAGACAT AAATTCCCCA TCTACATCCT TCCCAAAGAC
    GACACGCCGC CTTTTTTAAT CAACAACGTG GCCTTCGAGG TGCAAGAAGG TGGCACGGTT
    AGGGTGGAGG AGTACATGCT GATGGCCTCA GATCTGGACT CCAGCGACGA CTACATTCAG
    TATCAGCTGA TTACTTTTCC CAGAGCTGGA CAGCTGATTA AGAAGTCTTC TCCACATGAA
    CCAGGTCTCC CAGTCAAGAG TTTCCTGCAG AGGGACCTTT TCCAAGGGCT AATCTACTAC
    AAGCACTCGG GAGAGGAAGT GTTTGAGGAC TCGTTTGATT TCATCCTCTC AGATGTCCAT
    CAGCCTCCAA ACCTGTCGGA CAGACATACT GTGGTGATCC ACGTGTTTCC AGTGAAGGAC
    CAGCTGCCTG TGGAAGTTTC TGGAACGGTT CGCTCCATCA CGGTTAAGGA AACGGAAGTT
    GTTTACATCA CTCAGAGCCT TCTGCACTTC AGAGATACAG AGCACCCGGA TACTGACCTC
    ATGTATGTGA TCACCCACCC CTGCTTCAGT CCAGGAAACA CCAGGTTATC AGATGCTGGC
    AGGCTGTTTT ATACTGACAG CACAAATGCA ATGAAAAAAG ATGCTATGGT TCCAGTGCTG
    AAGTCCTTTA CCCAGCACTT TATTAATGTA CAATATCATT TTGCTGCCTC TTCTGTAAGT
    TCTCTTTTTT GTTGTCCGCA GCATGCAGTG AACCACCACA AAGTGGCATA CATGCCTCCA
    ATAGAGGATA TTGGGCCTGA GCCTCTGTTT GTGCAGTTTG TGTTTTCTGT GAGTGACCAT
    CAGGGCGGCG CTCTTACAGG ACTCACCTTC AACATCACAG TCACGCCTGT TGACAACCAG
    GCCCCAGAAA TGTTTACTAA CTTGCTGAGG GTTGAGGAAG GTGGTGGTAG CTTCCTGATG
    GAGGAGCATC TTTTGGTTCA GGATGTGGAC AGCTCGGAAG ATCAGCTCAG GATACACATG
    AAGACCAAAC CCCAACATGG ACGGCTGGAG CTGCAGGGAA CGGCCATGCT AGAAGGAGAC
    AGCTTCACTC TGCGGGACCT CAAAGCATTG AGGGTCAGAT ACATTCATGA TGACTCTGAG
    ACCCAAAAGG ATAGCATTGG ACTTACAATC ACAGACGGAA TCAACTCAGC ACATGGAGCA
    CTTTTAATTC AGATCCTGCC TGTGAATGAT GAACCGCCAC AGTTGGGCTC TGATTTGAAG
    GCAAAGCTGT CCTGCAAAGA GGGGGGTCAG GTTCAGATTA CGGTCGAGTA CCTCTCTGCT
    ACAGATGTTG ACAGCGATGA CACTCGGCTA ACATACATGC TTGCCAGAAC ACCTGGGCGT
    GGGGTCATAC AGCGAAACGG GGTCACTGTG GACAAGTTCT CCCAGCTGGA TGTGCTCAAT
    GGACTCATCT TCTACCTGCA CACAGGTGGT GAGATTGGAC CGGATCCTGT CTCGGACACT
    GTCACCCTCA TTGTGTCAGA TGGAGAGGCT GGAACAATGG ACGGCTGCTG CCAGGAGGAC
    GCTCTCCCCC CTCCCGTCCC CCTGCATGGC ACGCTTCCGG TCTATGACCT GAACATCACT
    GTGATGCCTG TCAACAACCA GGTCCCAACC ATTACGCTGG GCGGCATGAT TGTGGTAGAT
    GAAGGTGCCA GAGCATGTCT GTGTGGGGGG GTGTTGGAGG CCAGCGATCC TGATAGTCGT
    CCAGAAGAAC TCATGTTTCA CTTGGTCACC CCGCCTCAGT ATGGGTTCCT GGAGAACACC
    CTTCCATCCC CTGGCTTCGA GAAAAGCAAT GCTGGACTGA GAGTGGTTTT TTTCAGCCAG
    CTCCACCTCA GTTCGGGTTT CATCAACTAT GTCCAGTCGG TGCACCAGGG TGTGGAGCCT
    ACAGCAGACT TCTTTACCAT CAGTGTCAGT GACGGAATGC AAAGATCTGC ACCATTGCAC
    GTCTACATCA TCATCAACCC CACCAATGAT GAGGTTCCAT CACTGCTGCT TAGCAACTTC
    ACAGTCATGG AGGGAGGAAT GAAGGATCTC AGCCCTGATA TTCTAAACGC AGTGGACGTT
    GATATCCCAG CAGAAAGTCT CACCCTGACC ATCTTGGATC CCCCAGCTCA TGGCACTCTG
    ATCAATGGCA TATATGGTCT TGAGATGAAT CGTTACAAAA GCATGAACCC AGAGGTTCTC
    CAACAAACCT TAGCAATCCA GTCCTTCACC TTGGAGGAGC TACAGCAAGG AATGAAGATC
    ATGTATATGC ACGATGACAC AGAAACCCTC AAGGACGCTT TCACCATACA GTTGACAGAT
    GGTGGGCACA CAGTCCAGGG GACTGCGTGT GTCCGCATCA TACCAGTCAA TGATGAAAAG
    CCATGGCTAC TAAAAAATGC AGGCGTTGAG GTCGAGGCCC TGGAGAAGAG GGTAATCTCC
    AGTGTAGTGC TGGAAGCAGG GGATCAGGAC ACTCCCAGTG ACCATCTTCT CTACATCCTC
    AACGCAGGTC CAAGATTTGG CCTTCTTCAG CTCAGGGTTA AGGCTGGGTG GGTTGATCTG
    AGCCCAGGTC AGAATTTCAC CCAGGAGGAT GTGGAGATGA ATCGCCTGTG GTACTCTCAC
    ACTACAGTTA GTGGCTTCAA AGGTCACGAC AGGTTTCACT TTGTCCTCAC CGATGGGGAA
    AACACAACAC CTCCACAGAG TTTCTTCATC TCGGTCCGCA CAGTTCAGAA GGGCGACATT
    GCTCTCATCA CTAAGCCTGT GACCTTGCTG GAGGGAGAGA GAGTCATTCT GACCACTGAC
    ATTCTGACGG CCGCAGACAG TGGAGGCCGG CCAGATGAGC TCATATACAC TGTAGCAGTG
    CCACCTGAAC ATGGTCACCT GCACATGGTC CAAACGCCAG GAGTACCAGT GTTCTCCTTC
    TCCCAAATGG ATGTGGCTGC CAACCGGGTA TGCTACACTC ATGACAACAG CCGTTTTGCT
    GACCGTGATT CTTTCAGCTT TGCCATTAGT AACGGTGTAG CTTCCAGAAG TGGTACAGTT
    CATTTCACCA TTGAACACAG TGACCGGATC CCACCAACCC TGAGCACCAA CAAAGGCCTT
    GAGCTCACAG AGGGCTCTAT GAAGACCATC TCCACGGAAG AGCTAAAACT GACTGATCCA
    GATACAGCTC TAGAGAATCT CACCTATGTA GTTACTCAAA GCCCACAGTA TGGAAAGCTG
    CTCTTTAAAG GATTGCCTCT CTCCAAGCCT CGTTTCACAC AACTGGACAT CAACAATATG
    GATCTAGCTT ACCAACATCT GAATGGACGA GCCACGATCG ACAGGTTCGC CTTTCAGCCC
    ACTGATGGAA CGAACAAGGG CTATTTGGAG TACGGACAGC TAAAGAGTGA CCCGGCTGTT
    TTTACAATAC AGATAGAGAT ACTGGACAAG ACGCCACCCA GTATTATAAA CAAAGGCATC
    CCAAGTACTG TGGAGAACCT TCCGGATGGC AAACATGGCA TCTATATCAC TTCCAAAGAG
    CTGCAGGCCT CAGATCCAGA CAGTCCCGAC GACACACTCG AGTTCACCAT CACTAGACCT
    CCACACTTCG GCTTCCTCGA AAACACCCTC ACAGGCGCGT TTATCAAAGG ACGCTTCACT
    CAGAAGGATG TGGACCAGAA GGCTGTGCGC TATGTCATTC CTGTGGATGT GGAAGTGACG
    GCGGACAGCT TTGAATTTCA AGTCACAGAC CCAGCAGGAA ACACAATACT GCCTGAAGTG
    CTGGAGCTGC GCTGGTCTCG TGTGGATCTG TCAGCTTCCT GTTACCGTGT GTGTGAGACT
    GCAGGAACTC TGGCTGTGCA GGTTCTGCGC TCCGGAAACA GCAAAGATCC GGCTTACATC
    GGCATACAGG TGGAAGAAGG AACAGCCAAA GTCGGGAAAG ACTTCACTCA CAGCTCGGCC
    AGTCTGATAC AGTTCGATCC AGGTGTGAAT GTCAAAATGT GGAACATTTA CCTGAAAGAT
    GATGGACTGG AAGAGAACCA TGAGAGATTT GACATCATTC TGAAGGCTCC AAAAAATGCT
    GTTTTGGGAC AGAGGAACAA GGCCTCTGTT GAGATTGTGG ATCCTAGAAA TGGCAGGTGT
    AATCCAGATG ACCTTATTGT GGAGGAAGAT GAGAATCAAC ATACTTACCA AATCCAGCCA
    CACATCCCAG AGCCAGAAAC ACCCGTGCTT GAGGAGTACA CTCCGGACCC AAGAAGCGGG
    ATCCTCTGGG AGAACTATCC TCCAAGAGGA GACGTCCCGT ATCGCACTGA TTTTAACCAT
    TACAGCCTTA CAGGTCAACA TATGGAGCAG GAGGCCTTTC ACAGTCCAGG CCGGAGACAA
    CTGAGGGTCC TGGAAGGAAA CAGACAGAGA GCTGGTCTGT CAGAGGTGGA CAGCAGAAAT
    CAGGAAAGGG TTTGGAGATT TCACGGTGTG ATCCCGGTGA GCCAGCAGGA AGCGGCTCCT
    CGTGTTCACT CTGAACTGGA GATCACTCCC ATCTGGAGCT GGCCTGGAGA TTCAGCAGAA
    ACCCCTCAGC GTGACTCCGC CTCTGACATT CAACAACACA AATCTCAGAC ATCTGTTAGC
    ATCGATTGTC CAAATGGTTG GACTCACTAC CGGAGGAACT GCTATATTTT GGGGCCTGGT
    ACCGCTAGCT GGAGTAGCGC ACAACATGCC TGCACACTGC TTGAAGGCCA ATTGACAGGT
    ATCCACTCAA AGAATGATAT GAAGTGGATC TGGAAGTTTG CTGGGAAGCA GCCATTTTGG
    ATTGGACTGG TAGGTGGTCC GGAGCACGGA TGGATATGGA CGGATGGGAA AGTGCTGTCG
    TTCTCCAGAC TCAGGAAAGA TGAAGAGGAT CAAACTCGCT CTGTTTGTGT TTTAGCTCAG
    AGCCAGAAGA TGTGGATTCC TAAAGGTTGC ATTGATGGAT CCGAACACAG ATACGTTTGT
    TCAGCACCTG CACAAATCAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001131130.1
    CDS1..6504
    Translation

    Target ORF information:

    RefSeq Version NM_001137658.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Fras1 related extracellular matrix 1b (frem1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001137658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGGGGTTT TCTTCCTCGC TCTAACAGTG CTCACCTCGA GTCACCTGTG GTCACATGTC 
    CAAGCTTCGA GTCTAGTGCA GTTAAATCGA GTTATTCGAG TAGCCAGAGG ACAATCTGTG
    TTCATCACAG CCAACGAACT TCAGTTCCAC ATGGATCACA AATCCGAGGC CTGTAAAGTA
    GAAGTGGTGC TTAATGAACC CATCACACAA AGAGTGGGAA AACTCACTCC ACAGGTTTTC
    GACTGCCAGT TCTTTCCAGA TGAGGTGAAG TATGTCCACA ATGGCAGTCC ACTTCTTGAG
    GAAGACACCG TCATGTTGAG AGTCTACAGG TTTACAGACA CCGAGACTTT TGTTGAGTCT
    ATGTTGCTGA AGGTACGTGT GTTTGAGCCT CAGAGAAGCC TTGTAGAGCT TGGAAATGTC
    CCTCTGGTGG TTCCTGAGTT CTATGGCCTT TCCAATGCCA TCAACGCCAG CGTTCTCACC
    TTTAAAACCC AGCCAGATGT GATCTGCACG CTTCGTCTTC TCAGCTCTGA GATCAGTGTT
    CCAGCACTGG GTCAGCTGGT GGTTGAGGAG TCTGGAACAA TGGTGGACAC TGAGGCTGGA
    CCAAGAAAAG TGTTTTTTTG TCAGTTTTTT TTTGGACGTC ACACTGCAAT CGCCTGCCCT
    GGAAATAAAG CCTGTGACCT CAAGACAAGG CAAGTGGAGT TCCTGAAGAC CGACTGCAGT
    GAATTCCTCT CTTCAGGTCT GAAGTACCAG CATCTCAGTC CTCCTTCACC TGAGATTGAC
    TACATCCCAG TCCGGCTGGA GTTTCGAGAC CAGACCTCCC GAGCCCTGCT AGAGACCGAG
    AGTGTCTGGT TACCGGTATT GATCCAGGGG GCGATGCAGA ACCAGCCGCC TCACGCTGCC
    TTCATGTCCA CCTTCATCCT GGAGGTAGAT CAGTTCATCC TGACGCCTCT GAGCACCGCT
    GCGCTGGATG CCAAAGACGA CGAGACACCG CAGGACCAGC TCATTTTCAG TGTCACCAAA
    CCTCCAGCCG AAGGATATAT AACTCATTTA GATGATCACA CTAAAATGGC CTCCTCCTTC
    TCATGGCAAG ACCTGAATGA GATGAAAATC GCATACCAGC CCCCAAACAG CAGTCATACA
    GCCAGGAGGA ACTATGAGGT TGAATTTCAG GCCATTGATG GCTCATTTGC CCCGAGTCTT
    CCTATCCTGA TGCACATCTC CATACGCACT GCAGAGACAA ACGCACCTCG AGTCTCCTGG
    AATATGGGCT TGGATCTGCT GGAAGGCCAA TCCAGACCAA TCACATGGGA GCATCTACAG
    ATTGTAGATA AGGACAATAT TGACGCCGTC ACCTTGATCG CAATGGACGG TCCACTTAAT
    GGACACTTAA GTGTTAGAGG TGGAAAAGGC TTCATGTTTA AGGTCAAGGA TCTCCGAGAG
    GGCGTTGTGG TCTATCATCA CTCGGACAGC GACACCACCA GGGATTACGT CGTCTTCCGA
    ATCACAGATG GCCGCCACAG CATCAGACAT AAATTCCCCA TCTACATCCT TCCCAAAGAC
    GACACGCCGC CTTTTTTAAT CAACAACGTG GCCTTCGAGG TGCAAGAAGG TGGCACGGTT
    AGGGTGGAGG AGTACATGCT GATGGCCTCA GATCTGGACT CCAGCGACGA CTACATTCAG
    TATCAGCTGA TTACTTTTCC CAGAGCTGGA CAGCTGATTA AGAAGTCTTC TCCACATGAA
    CCAGGTCTCC CAGTCAAGAG TTTCCTGCAG AGGGACCTTT TCCAAGGGCT AATCTACTAC
    AAGCACTCGG GAGAGGAAGT GTTTGAGGAC TCGTTTGATT TCATCCTCTC AGATGTCCAT
    CAGCCTCCAA ACCTGTCGGA CAGACATACT GTGGTGATCC ACGTGTTTCC AGTGAAGGAC
    CAGCTGCCTG TGGAAGTTTC TGGAACGGTT CGCTCCATCA CGGTTAAGGA AACGGAAGTT
    GTTTACATCA CTCAGAGCCT TCTGCACTTC AGAGATACAG AGCACCCGGA TACTGACCTC
    ATGTATGTGA TCACCCACCC CTGCTTCAGT CCAGGAAACA CCAGGTTATC AGATGCTGGC
    AGGCTGTTTT ATACTGACAG CACAAATGCA ATGAAAAAAG ATGCTATGGT TCCAGTGCTG
    AAGTCCTTTA CCCAGCACTT TATTAATGTA CAATATCATT TTGCTGCCTC TTCTGTAAGT
    TCTCTTTTTT GTTGTCCGCA GCATGCAGTG AACCACCACA AAGTGGCATA CATGCCTCCA
    ATAGAGGATA TTGGGCCTGA GCCTCTGTTT GTGCAGTTTG TGTTTTCTGT GAGTGACCAT
    CAGGGCGGCG CTCTTACAGG ACTCACCTTC AACATCACAG TCACGCCTGT TGACAACCAG
    GCCCCAGAAA TGTTTACTAA CTTGCTGAGG GTTGAGGAAG GTGGTGGTAG CTTCCTGATG
    GAGGAGCATC TTTTGGTTCA GGATGTGGAC AGCTCGGAAG ATCAGCTCAG GATACACATG
    AAGACCAAAC CCCAACATGG ACGGCTGGAG CTGCAGGGAA CGGCCATGCT AGAAGGAGAC
    AGCTTCACTC TGCGGGACCT CAAAGCATTG AGGGTCAGAT ACATTCATGA TGACTCTGAG
    ACCCAAAAGG ATAGCATTGG ACTTACAATC ACAGACGGAA TCAACTCAGC ACATGGAGCA
    CTTTTAATTC AGATCCTGCC TGTGAATGAT GAACCGCCAC AGTTGGGCTC TGATTTGAAG
    GCAAAGCTGT CCTGCAAAGA GGGGGGTCAG GTTCAGATTA CGGTCGAGTA CCTCTCTGCT
    ACAGATGTTG ACAGCGATGA CACTCGGCTA ACATACATGC TTGCCAGAAC ACCTGGGCGT
    GGGGTCATAC AGCGAAACGG GGTCACTGTG GACAAGTTCT CCCAGCTGGA TGTGCTCAAT
    GGACTCATCT TCTACCTGCA CACAGGTGGT GAGATTGGAC CGGATCCTGT CTCGGACACT
    GTCACCCTCA TTGTGTCAGA TGGAGAGGCT GGAACAATGG ACGGCTGCTG CCAGGAGGAC
    GCTCTCCCCC CTCCCGTCCC CCTGCATGGC ACGCTTCCGG TCTATGACCT GAACATCACT
    GTGATGCCTG TCAACAACCA GGTCCCAACC ATTACGCTGG GCGGCATGAT TGTGGTAGAT
    GAAGGTGCCA GAGCATGTCT GTGTGGGGGG GTGTTGGAGG CCAGCGATCC TGATAGTCGT
    CCAGAAGAAC TCATGTTTCA CTTGGTCACC CCGCCTCAGT ATGGGTTCCT GGAGAACACC
    CTTCCATCCC CTGGCTTCGA GAAAAGCAAT GCTGGACTGA GAGTGGTTTT TTTCAGCCAG
    CTCCACCTCA GTTCGGGTTT CATCAACTAT GTCCAGTCGG TGCACCAGGG TGTGGAGCCT
    ACAGCAGACT TCTTTACCAT CAGTGTCAGT GACGGAATGC AAAGATCTGC ACCATTGCAC
    GTCTACATCA TCATCAACCC CACCAATGAT GAGGTTCCAT CACTGCTGCT TAGCAACTTC
    ACAGTCATGG AGGGAGGAAT GAAGGATCTC AGCCCTGATA TTCTAAACGC AGTGGACGTT
    GATATCCCAG CAGAAAGTCT CACCCTGACC ATCTTGGATC CCCCAGCTCA TGGCACTCTG
    ATCAATGGCA TATATGGTCT TGAGATGAAT CGTTACAAAA GCATGAACCC AGAGGTTCTC
    CAACAAACCT TAGCAATCCA GTCCTTCACC TTGGAGGAGC TACAGCAAGG AATGAAGATC
    ATGTATATGC ACGATGACAC AGAAACCCTC AAGGACGCTT TCACCATACA GTTGACAGAT
    GGTGGGCACA CAGTCCAGGG GACTGCGTGT GTCCGCATCA TACCAGTCAA TGATGAAAAG
    CCATGGCTAC TAAAAAATGC AGGCGTTGAG GTCGAGGCCC TGGAGAAGAG GGTAATCTCC
    AGTGTAGTGC TGGAAGCAGG GGATCAGGAC ACTCCCAGTG ACCATCTTCT CTACATCCTC
    AACGCAGGTC CAAGATTTGG CCTTCTTCAG CTCAGGGTTA AGGCTGGGTG GGTTGATCTG
    AGCCCAGGTC AGAATTTCAC CCAGGAGGAT GTGGAGATGA ATCGCCTGTG GTACTCTCAC
    ACTACAGTTA GTGGCTTCAA AGGTCACGAC AGGTTTCACT TTGTCCTCAC CGATGGGGAA
    AACACAACAC CTCCACAGAG TTTCTTCATC TCGGTCCGCA CAGTTCAGAA GGGCGACATT
    GCTCTCATCA CTAAGCCTGT GACCTTGCTG GAGGGAGAGA GAGTCATTCT GACCACTGAC
    ATTCTGACGG CCGCAGACAG TGGAGGCCGG CCAGATGAGC TCATATACAC TGTAGCAGTG
    CCACCTGAAC ATGGTCACCT GCACATGGTC CAAACGCCAG GAGTACCAGT GTTCTCCTTC
    TCCCAAATGG ATGTGGCTGC CAACCGGGTA TGCTACACTC ATGACAACAG CCGTTTTGCT
    GACCGTGATT CTTTCAGCTT TGCCATTAGT AACGGTGTAG CTTCCAGAAG TGGTACAGTT
    CATTTCACCA TTGAACACAG TGACCGGATC CCACCAACCC TGAGCACCAA CAAAGGCCTT
    GAGCTCACAG AGGGCTCTAT GAAGACCATC TCCACGGAAG AGCTAAAACT GACTGATCCA
    GATACAGCTC TAGAGAATCT CACCTATGTA GTTACTCAAA GCCCACAGTA TGGAAAGCTG
    CTCTTTAAAG GATTGCCTCT CTCCAAGCCT CGTTTCACAC AACTGGACAT CAACAATATG
    GATCTAGCTT ACCAACATCT GAATGGACGA GCCACGATCG ACAGGTTCGC CTTTCAGCCC
    ACTGATGGAA CGAACAAGGG CTATTTGGAG TACGGACAGC TAAAGAGTGA CCCGGCTGTT
    TTTACAATAC AGATAGAGAT ACTGGACAAG ACGCCACCCA GTATTATAAA CAAAGGCATC
    CCAAGTACTG TGGAGAACCT TCCGGATGGC AAACATGGCA TCTATATCAC TTCCAAAGAG
    CTGCAGGCCT CAGATCCAGA CAGTCCCGAC GACACACTCG AGTTCACCAT CACTAGACCT
    CCACACTTCG GCTTCCTCGA AAACACCCTC ACAGGCGCGT TTATCAAAGG ACGCTTCACT
    CAGAAGGATG TGGACCAGAA GGCTGTGCGC TATGTCATTC CTGTGGATGT GGAAGTGACG
    GCGGACAGCT TTGAATTTCA AGTCACAGAC CCAGCAGGAA ACACAATACT GCCTGAAGTG
    CTGGAGCTGC GCTGGTCTCG TGTGGATCTG TCAGCTTCCT GTTACCGTGT GTGTGAGACT
    GCAGGAACTC TGGCTGTGCA GGTTCTGCGC TCCGGAAACA GCAAAGATCC GGCTTACATC
    GGCATACAGG TGGAAGAAGG AACAGCCAAA GTCGGGAAAG ACTTCACTCA CAGCTCGGCC
    AGTCTGATAC AGTTCGATCC AGGTGTGAAT GTCAAAATGT GGAACATTTA CCTGAAAGAT
    GATGGACTGG AAGAGAACCA TGAGAGATTT GACATCATTC TGAAGGCTCC AAAAAATGCT
    GTTTTGGGAC AGAGGAACAA GGCCTCTGTT GAGATTGTGG ATCCTAGAAA TGGCAGGTGT
    AATCCAGATG ACCTTATTGT GGAGGAAGAT GAGAATCAAC ATACTTACCA AATCCAGCCA
    CACATCCCAG AGCCAGAAAC ACCCGTGCTT GAGGAGTACA CTCCGGACCC AAGAAGCGGG
    ATCCTCTGGG AGAACTATCC TCCAAGAGGA GACGTCCCGT ATCGCACTGA TTTTAACCAT
    TACAGCCTTA CAGGTCAACA TATGGAGCAG GAGGCCTTTC ACAGTCCAGG CCGGAGACAA
    CTGAGGGTCC TGGAAGGAAA CAGACAGAGA GCTGGTCTGT CAGAGGTGGA CAGCAGAAAT
    CAGGAAAGGG TTTGGAGATT TCACGGTGTG ATCCCGGTGA GCCAGCAGGA AGCGGCTCCT
    CGTGTTCACT CTGAACTGGA GATCACTCCC ATCTGGAGCT GGCCTGGAGA TTCAGCAGAA
    ACCCCTCAGC GTGACTCCGC CTCTGACATT CAACAACACA AATCTCAGAC ATCTGTTAGC
    ATCGATTGTC CAAATGGTTG GACTCACTAC CGGAGGAACT GCTATATTTT GGGGCCTGGT
    ACCGCTAGCT GGAGTAGCGC ACAACATGCC TGCACACTGC TTGAAGGCCA ATTGACAGGT
    ATCCACTCAA AGAATGATAT GAAGTGGATC TGGAAGTTTG CTGGGAAGCA GCCATTTTGG
    ATTGGACTGG TAGGTGGTCC GGAGCACGGA TGGATATGGA CGGATGGGAA AGTGCTGTCG
    TTCTCCAGAC TCAGGAAAGA TGAAGAGGAT CAAACTCGCT CTGTTTGTGT TTTAGCTCAG
    AGCCAGAAGA TGTGGATTCC TAAAGGTTGC ATTGATGGAT CCGAACACAG ATACGTTTGT
    TCAGCACCTG CACAAATCAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42552
    Clone ID Related Accession (Same CDS sequence) XM_009296245.3 , XM_009296245.2
    Accession Version XM_009296245.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product fras-related extracellular matrix protein 1b isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGGGTTT TCTTCCTCGC TCTAACAGTG CTCACCTCGA GTCACCTGTG GTCACATGTC 
    CAAGCTTCGA GTCTAGTGCA GTTAAATCGA GTTATTCGAG TAGCCAGAGG ACAATCTGTG
    TTCATCACAG CCAACGAACT TCAGTTCCAC ATGGATCACA AATCCGAGGC CTGTAAAGTA
    GAAGTGGTGC TTAATGAACC CATCACACAA AGAGTGGGAA AACTCACTCC ACAGGTTTTC
    GACTGCCAGT TCTTTCCAGA TGAGGTGAAG TATGTCCACA ATGGCAGTCC ACTTCTTGAG
    GAAGACACCG TCATGTTGAG AGTCTACAGG TTTACAGACA CCGAGACTTT TGTTGAGTCT
    ATGTTGCTGA AGGTACGTGT GTTTGAGCCT CAGAGAAGCC TTGTAGAGCT TGGAAATGTC
    CCTCTGGTGG TTCCTGAGTT CTATGGCCTT TCCAATGCCA TCAACGCCAG CGTTCTCACC
    TTTAAAACCC AGCCAGATGT GATCTGCACG CTTCGTCTTC TCAGCTCTGA GATCAGTGTT
    CCAGCACTGG GTCAGCTGGT GGTTGAGGAG TCTGGAACAA TGGTGGACAC TGAGGCTGGA
    CCAAGAAAAG GACGTCACAC TGCAATCGCC TGCCCTGGAA ATAAAGCCTG TGACCTCAAG
    ACAAGGCAAG TGGAGTTCCT GAAGACCGAC TGCAGTGAAT TCCTCTCTTC AGGTCTGAAG
    TACCAGCATC TCAGTCCTCC TTCACCTGAG ATTGACTACA TCCCAGTCCG GCTGGAGTTT
    CGAGACCAGA CCTCCCGAGC CCTGCTAGAG ACCGAGAGTG TCTGGTTACC GGTATTGATC
    CAGGGGGCGA TGCAGAACCA GCCGCCTCAC GCTGCCTTCA TGTCCACCTT CATCCTGGAG
    GTAGATCAGT TCATCCTGAC GCCTCTGAGC ACCGCTGCGC TGGATGCCAA AGACGACGAG
    ACACCGCAGG ACCAGCTCAT TTTCAGTGTC ACCAAACCTC CAGCCGAAGG ATATATAACT
    CATTTAGATG ATCACACTAA AATGGCCTCC TCCTTCTCAT GGCAAGACCT GAATGAGATG
    AAAATCGCAT ACCAGCCCCC AAACAGCAGT CATACAGCCA GGAGGAACTA TGAGGTTGAA
    TTTCAGGCCA TTGATGGCTC ATTTGCCCCG AGTCTTCCTA TCCTGATGCA CATCTCCATA
    CGCACTGCAG AGACAAACGC ACCTCGAGTC TCCTGGAATA TGGGCTTGGA TCTGCTGGAA
    GGCCAATCCA GACCAATCAC ATGGGAGCAT CTACAGATTG TAGATAAGGA CAATATTGAC
    GCCGTCACCT TGATCGCAAT GGACGGTCCA CTTAATGGAC ACTTAAGTGT TAGAGGTGGA
    AAAGGCTTCA TGTTTAAGGT CAAGGATCTC CGAGAGGGCG TTGTGGTCTA TCATCACTCG
    GACAGCGACA CCACCAGGGA TTACGTCGTC TTCCGAATCA CAGATGGCCG CCACAGCATC
    AGACATAAAT TCCCCATCTA CATCCTTCCC AAAGACGACA CGCCGCCTTT TTTAATCAAC
    AACGTGGCCT TCGAGGTGCA AGAAGGTGGC ACGGTTAGGG TGGAGGAGTA CATGCTGATG
    GCCTCAGATC TGGACTCCAG CGACGACTAC ATTCAGTATC AGCTGATTAC TTTTCCCAGA
    GCTGGACAGC TGATTAAGAA GTCTTCTCCA CATGAACCAG GTCTCCCAGT CAAGAGTTTC
    CTGCAGAGGG ACCTTTTCCA AGGGCTAATC TACTACAAGC ACTCGGGAGA GGAAGTGTTT
    GAGGACTCGT TTGATTTCAT CCTCTCAGAT GTCCATCAGC CTCCAAACCT GTCGGACAGA
    CATACTGTGG TGATCCACGT GTTTCCAGTG AAGGACCAGC TGCCTGTGGA AGTTTCTGGA
    ACGGTTCGCT CCATCACGGT TAAGGAAACG GAAGTTGTTT ACATCACTCA GAGCCTTCTG
    CACTTCAGAG ATACAGAGCA CCCGGATACT GACCTCATGT ATGTGATCAC CCACCCCTGC
    TTCAGTCCAG GAAACACCAG GTTATCAGAT GCTGGCAGGC TGTTTTATAC TGACAGCACA
    AATGCAATGA AAAAAGATGC TATGGTTCCA GTGCTGAAGT CCTTTACCCA GCATGCAGTG
    AACCACCACA AAGTGGCATA CATGCCTCCA ATAGAGGATA TTGGGCCTGA GCCTCTGTTT
    GTGCAGTTTG TGTTTTCTGT GAGTGACCAT CAGGGCGGCG CTCTTACAGG ACTCACCTTC
    AACATCACAG TCACGCCTGT TGACAACCAG GCCCCAGAAA TGTTTACTAA CTTGCTGAGG
    GTTGAGGAAG GTGGTGGTAG CTTCCTGATG GAGGAGCATC TTTTGGTTCA GGATGTGGAC
    AGCTCGGAAG ATCAGCTCAG GATACACATG AAGACCAAAC CCCAACATGG ACGGCTGGAG
    CTGCAGGGAA CGGCCATGCT AGAAGGAGAC AGCTTCACTC TGCGGGACCT CAAAGCATTG
    AGGGTCAGAT ACATTCATGA TGACTCTGAG ACCCAAAAGG ATAGCATTGG ACTTACAATC
    ACAGACGGAA TCAACTCAGC ACATGGAGCA CTTTTAATTC AGATCCTGCC TGTGAATGAT
    GAACCGCCAC AGTTGGGCTC TGATTTGAAG GCAAAGCTGT CCTGCAAAGA GGGGGGTCAG
    GTTCAGATTA CGGTCGAGTA CCTCTCTGCT ACAGATGTTG ACAGCGATGA CACTCGGCTA
    ACATACATGC TTGCCAGAAC ACCTGGGCGT GGGGTCATAC AGCGAAACGG GGTCACTGTG
    GACAAGTTCT CCCAGCTGGA TGTGCTCAAT GGACTCATCT TCTACCTGCA CACAGGTGGT
    GAGATTGGAC CGGATCCTGT CTCGGACACT GTCACCCTCA TTGTGTCAGA TGGAGAGGCT
    GGAACAATGG ACGGCTGCTG CCAGGAGGAC GCTCTCCCCC CTCCCGTCCC CCTGCATGGC
    ACGCTTCCGG TCTATGACCT GAACATCACT GTGATGCCTG TCAACAACCA GGTCCCAACC
    ATTACGCTGG GCGGCATGAT TGTGGTAGAT GAAGGTGCCA GAGCATGTCT GTGTGGGGGG
    GTGTTGGAGG CCAGCGATCC TGATAGTCGT CCAGAAGAAC TCATGTTTCA CTTGGTCACC
    CCGCCTCAGT ATGGGTTCCT GGAGAACACC CTTCCATCCC CTGGCTTCGA GAAAAGCAAT
    GCTGGACTGA GAGTGGTTTT TTTCAGCCAG CTCCACCTCA GTTCGGGTTT CATCAACTAT
    GTCCAGTCGG TGCACCAGGG TGTGGAGCCT ACAGCAGACT TCTTTACCAT CAGTGTCAGT
    GACGGAATGC AAAGATCTGC ACCATTGCAC GTCTACATCA TCATCAACCC CACCAATGAT
    GAGGTTCCAT CACTGCTGCT TAGCAACTTC ACAGTCATGG AGGGAGGAAT GAAGGATCTC
    AGCCCTGATA TTCTAAACGC AGTGGACGTT GATATCCCAG CAGAAAGTCT CACCCTGACC
    ATCTTGGATC CCCCAGCTCA TGGCACTCTG ATCAATGGCA TATATGGTCT TGAGATGAAT
    CGTTACAAAA GCATGAACCC AGAGGTTCTC CAACAAACCT TAGCAATCCA GTCCTTCACC
    TTGGAGGAGC TACAGCAAGG AATGAAGATC ATGTATATGC ACGATGACAC AGAAACCCTC
    AAGGACGCTT TCACCATACA GTTGACAGAT GGTGGGCACA CAGTCCAGGG GACTGCGTGT
    GTCCGCATCA TACCAGTCAA TGATGAAAAG CCATGGCTAC TAAAAAATGC AGGCGTTGAG
    GTCGAGGCCC TGGAGAAGAG GGTAATCTCC AGTGTAGTGC TGGAAGCAGG GGATCAGGAC
    ACTCCCAGTG ACCATCTTCT CTACATCCTC AACGCAGGTC CAAGATTTGG CCTTCTTCAG
    CTCAGGACAG AGGCTGGGTG GGTTGATCTG AGCCCAGGTC AGAATTTCAC CCAGGAGGAT
    GTGGAGATGA ATCGCCTGTG GTACTCTCAC ACTACAGTTA GTGGCTTCAA AGGTCACGAC
    AGGTTTCACT TTGTCCTCAC CGATGGGGAA AACACAACAC CTCCACAGAG TTTCTTCATC
    TCGGTCCGCA CAGTTCAGAA GGGCGACATT GCTCTCATCA CTAAGCCTGT GACCTTGCTG
    GAGGGAGAGA GAGTCATTCT GACCACTGAC ATTCTGACGG CCGCAGACAG TGGAGGCCGG
    CCAGATGAGC TCATATACAC TGTAGCAGTG CCACCTGAAC ATGGTCACCT GCACATGGTC
    CAAACGCCAG GAGTACCAGT GTTCTCCTTC TCCCAAATGG ATGTGGCTGC CAACCGGGTA
    TGCTACACTC ATGACAACAG CCGTTTTGCT GACCGTGATT CTTTCAGCTT TGCCATTAGT
    AACGGTGTAG CTTCCAGAAG TGGTACAGTT CATTTCACCA TTGAACACAG TGACCGGATC
    CCACCAACCC TGAGCACCAA CAAAGGCCTT GAGCTCACAG AGGGCTCTAT GAAGACCATC
    TCCACGGAAG AGCTAAAACT GACTGATCCA GATACAGCTC TAGAGAATCT CACCTATGTA
    GTTACTCAAA GCCCACAGTA TGGAAAGCTG CTCTTTAAAG GATTGCCTCT CTCCAAGCCT
    CGTTTCACAC AACTGGACAT CAACAATATG GATCTAGCTT ACCAACATCT GAATGGACGA
    GCCACGATCG ACAGGTTCGC CTTTCAGCCC ACTGATGGAA CGAACAAGGG CTATTTGGAG
    TACGGACAGC TAAAGAGTGA CCCGGCTGTT TTTACAATAC AGATAGAGAT ACTGGACAAG
    ACGCCACCCA GTATTATAAA CAAAGGCATC CCAAGTACTG TGGAGAACCT TCCGGATGGC
    AAACATGGCA TCTATATCAC TTCCAAAGAG CTGCAGGCCT CAGATCCAGA CAGTCCCGAC
    GACACACTCG AGTTCACCAT CACTAGACCT CCACACTTCG GCTTCCTCGA AAACACCCTC
    ACAGGTGCGT TTATCAAAGG ACGCTTCACT CAGAAGGATG TGGACCAGAA GGCTGTGCGC
    TATGTCATTC CTGTGGATGT GGAAGTGACG GCGGACAGCT TTGAATTTCA AGTCACAGAC
    CCAGCAGGAA ACACAATACT GCCTGAAGTG CTGGAGCTGC GCTGGTCTCG TGTGGATCTG
    TCAGCTTCCT GTTACCGTGT GTGTGAGACT GCAGGAACTC TGGCTGTGCA GGTTCTGCGC
    TCCGGAAACA GCAAAGATCC GGCTTACATC GGCATACAGG TGGAAGAAGG AACAGCCAAA
    GTCGGGAAAG ACTTCACTCA CAGCTCGGCC AGTCTGATAC AGTTCGATCC AGGTGTGAAT
    GTCAAAATGT GGAACATTTA CCTGAAAGAT GATGGACTGG AAGAGAACCA TGAGAGATTT
    GACATCATTC TGAAGGCTCC AAAAAATGCT GTTTTGGGAC AGAGGAACAA GGCCTCTGTT
    GAGATTGTGG ATCCTAGAAA TGGCAGGTGT AATCCAGATG ACCTTATTGT GGAGGAAGAT
    GAGAATCAAC ATACTTACCA AATCCAGCCA CACATCCCAG AGCCAGAAAC ACCCGTGCTT
    GAGGAGTACA CTCCGGACCC AAGAAGCGGG ATCCTCTGGG AGAACTATCC TCCAAGAGGA
    GACGTCCCGT ATCGCACTGA TTTTAACCAT TACAGCCTTA CAGGTCAACA TATGGAGCAG
    GAGGCCTTTC ACAGTCCAGG CCGGAGACAA CTGAGGGTCC TGGAAGGAAA CAGACAGAGA
    GCTGGTCTGT CAGAGGTGGA CAGCAGAAAT CAGGAAAGGG TTTGGAGATT TCACGGTGTG
    ATCCCGGTGA GCCAGCAGGA AGCGGCTCCT CGTGTTCACT CTGAACTGGA GATCACTCCC
    ATCTGGAGCT GGCCTGGAGA TTCAGCAGAA ACCCCTCAGC GTGACTCCGC CTCTGACATT
    CAACAACACA AATCTCAGAC ATCTGTTAGC ATCGATTGTC CAAATGGTTG GACTCACTAC
    CGGAGGAACT GCTATATTTT GGGGCCTGGT ACCGCTAGCT GGAGTAGCGC ACAACATGCC
    TGCACACTGC TTGAAGGCCA ATTGACAGGT ATCCACTCAA AGAATGATAT GAAGTGGATC
    TGGAAGTTTG CTGGGAAGCA GCCATTTTGG ATTGGACTGG TAGGTGGTCC GGAGCACGGA
    TGGATATGGA CGGATGGGAA AGTGCTGTCG TTCTCCAGAC TCAGGAAAGA TGAAGAGGAT
    CAAACTCGCT CTGTTTGTGT TTTAGCTCAG AGCCAGAAGA TGTGGATTCC TAAAGGTTGC
    ATTGATGGAT CCGAACACAG ATACGTTTGT TCAGCACCTG CACAAATCAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009294520.1
    CDS478..6891
    Translation

    Target ORF information:

    RefSeq Version XM_009296245.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Fras1 related extracellular matrix 1b (frem1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296245.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGGGTTT TCTTCCTCGC TCTAACAGTG CTCACCTCGA GTCACCTGTG GTCACATGTC 
    CAAGCTTCGA GTCTAGTGCA GTTAAATCGA GTTATTCGAG TAGCCAGAGG ACAATCTGTG
    TTCATCACAG CCAACGAACT TCAGTTCCAC ATGGATCACA AATCCGAGGC CTGTAAAGTA
    GAAGTGGTGC TTAATGAACC CATCACACAA AGAGTGGGAA AACTCACTCC ACAGGTTTTC
    GACTGCCAGT TCTTTCCAGA TGAGGTGAAG TATGTCCACA ATGGCAGTCC ACTTCTTGAG
    GAAGACACCG TCATGTTGAG AGTCTACAGG TTTACAGACA CCGAGACTTT TGTTGAGTCT
    ATGTTGCTGA AGGTACGTGT GTTTGAGCCT CAGAGAAGCC TTGTAGAGCT TGGAAATGTC
    CCTCTGGTGG TTCCTGAGTT CTATGGCCTT TCCAATGCCA TCAACGCCAG CGTTCTCACC
    TTTAAAACCC AGCCAGATGT GATCTGCACG CTTCGTCTTC TCAGCTCTGA GATCAGTGTT
    CCAGCACTGG GTCAGCTGGT GGTTGAGGAG TCTGGAACAA TGGTGGACAC TGAGGCTGGA
    CCAAGAAAAG GACGTCACAC TGCAATCGCC TGCCCTGGAA ATAAAGCCTG TGACCTCAAG
    ACAAGGCAAG TGGAGTTCCT GAAGACCGAC TGCAGTGAAT TCCTCTCTTC AGGTCTGAAG
    TACCAGCATC TCAGTCCTCC TTCACCTGAG ATTGACTACA TCCCAGTCCG GCTGGAGTTT
    CGAGACCAGA CCTCCCGAGC CCTGCTAGAG ACCGAGAGTG TCTGGTTACC GGTATTGATC
    CAGGGGGCGA TGCAGAACCA GCCGCCTCAC GCTGCCTTCA TGTCCACCTT CATCCTGGAG
    GTAGATCAGT TCATCCTGAC GCCTCTGAGC ACCGCTGCGC TGGATGCCAA AGACGACGAG
    ACACCGCAGG ACCAGCTCAT TTTCAGTGTC ACCAAACCTC CAGCCGAAGG ATATATAACT
    CATTTAGATG ATCACACTAA AATGGCCTCC TCCTTCTCAT GGCAAGACCT GAATGAGATG
    AAAATCGCAT ACCAGCCCCC AAACAGCAGT CATACAGCCA GGAGGAACTA TGAGGTTGAA
    TTTCAGGCCA TTGATGGCTC ATTTGCCCCG AGTCTTCCTA TCCTGATGCA CATCTCCATA
    CGCACTGCAG AGACAAACGC ACCTCGAGTC TCCTGGAATA TGGGCTTGGA TCTGCTGGAA
    GGCCAATCCA GACCAATCAC ATGGGAGCAT CTACAGATTG TAGATAAGGA CAATATTGAC
    GCCGTCACCT TGATCGCAAT GGACGGTCCA CTTAATGGAC ACTTAAGTGT TAGAGGTGGA
    AAAGGCTTCA TGTTTAAGGT CAAGGATCTC CGAGAGGGCG TTGTGGTCTA TCATCACTCG
    GACAGCGACA CCACCAGGGA TTACGTCGTC TTCCGAATCA CAGATGGCCG CCACAGCATC
    AGACATAAAT TCCCCATCTA CATCCTTCCC AAAGACGACA CGCCGCCTTT TTTAATCAAC
    AACGTGGCCT TCGAGGTGCA AGAAGGTGGC ACGGTTAGGG TGGAGGAGTA CATGCTGATG
    GCCTCAGATC TGGACTCCAG CGACGACTAC ATTCAGTATC AGCTGATTAC TTTTCCCAGA
    GCTGGACAGC TGATTAAGAA GTCTTCTCCA CATGAACCAG GTCTCCCAGT CAAGAGTTTC
    CTGCAGAGGG ACCTTTTCCA AGGGCTAATC TACTACAAGC ACTCGGGAGA GGAAGTGTTT
    GAGGACTCGT TTGATTTCAT CCTCTCAGAT GTCCATCAGC CTCCAAACCT GTCGGACAGA
    CATACTGTGG TGATCCACGT GTTTCCAGTG AAGGACCAGC TGCCTGTGGA AGTTTCTGGA
    ACGGTTCGCT CCATCACGGT TAAGGAAACG GAAGTTGTTT ACATCACTCA GAGCCTTCTG
    CACTTCAGAG ATACAGAGCA CCCGGATACT GACCTCATGT ATGTGATCAC CCACCCCTGC
    TTCAGTCCAG GAAACACCAG GTTATCAGAT GCTGGCAGGC TGTTTTATAC TGACAGCACA
    AATGCAATGA AAAAAGATGC TATGGTTCCA GTGCTGAAGT CCTTTACCCA GCATGCAGTG
    AACCACCACA AAGTGGCATA CATGCCTCCA ATAGAGGATA TTGGGCCTGA GCCTCTGTTT
    GTGCAGTTTG TGTTTTCTGT GAGTGACCAT CAGGGCGGCG CTCTTACAGG ACTCACCTTC
    AACATCACAG TCACGCCTGT TGACAACCAG GCCCCAGAAA TGTTTACTAA CTTGCTGAGG
    GTTGAGGAAG GTGGTGGTAG CTTCCTGATG GAGGAGCATC TTTTGGTTCA GGATGTGGAC
    AGCTCGGAAG ATCAGCTCAG GATACACATG AAGACCAAAC CCCAACATGG ACGGCTGGAG
    CTGCAGGGAA CGGCCATGCT AGAAGGAGAC AGCTTCACTC TGCGGGACCT CAAAGCATTG
    AGGGTCAGAT ACATTCATGA TGACTCTGAG ACCCAAAAGG ATAGCATTGG ACTTACAATC
    ACAGACGGAA TCAACTCAGC ACATGGAGCA CTTTTAATTC AGATCCTGCC TGTGAATGAT
    GAACCGCCAC AGTTGGGCTC TGATTTGAAG GCAAAGCTGT CCTGCAAAGA GGGGGGTCAG
    GTTCAGATTA CGGTCGAGTA CCTCTCTGCT ACAGATGTTG ACAGCGATGA CACTCGGCTA
    ACATACATGC TTGCCAGAAC ACCTGGGCGT GGGGTCATAC AGCGAAACGG GGTCACTGTG
    GACAAGTTCT CCCAGCTGGA TGTGCTCAAT GGACTCATCT TCTACCTGCA CACAGGTGGT
    GAGATTGGAC CGGATCCTGT CTCGGACACT GTCACCCTCA TTGTGTCAGA TGGAGAGGCT
    GGAACAATGG ACGGCTGCTG CCAGGAGGAC GCTCTCCCCC CTCCCGTCCC CCTGCATGGC
    ACGCTTCCGG TCTATGACCT GAACATCACT GTGATGCCTG TCAACAACCA GGTCCCAACC
    ATTACGCTGG GCGGCATGAT TGTGGTAGAT GAAGGTGCCA GAGCATGTCT GTGTGGGGGG
    GTGTTGGAGG CCAGCGATCC TGATAGTCGT CCAGAAGAAC TCATGTTTCA CTTGGTCACC
    CCGCCTCAGT ATGGGTTCCT GGAGAACACC CTTCCATCCC CTGGCTTCGA GAAAAGCAAT
    GCTGGACTGA GAGTGGTTTT TTTCAGCCAG CTCCACCTCA GTTCGGGTTT CATCAACTAT
    GTCCAGTCGG TGCACCAGGG TGTGGAGCCT ACAGCAGACT TCTTTACCAT CAGTGTCAGT
    GACGGAATGC AAAGATCTGC ACCATTGCAC GTCTACATCA TCATCAACCC CACCAATGAT
    GAGGTTCCAT CACTGCTGCT TAGCAACTTC ACAGTCATGG AGGGAGGAAT GAAGGATCTC
    AGCCCTGATA TTCTAAACGC AGTGGACGTT GATATCCCAG CAGAAAGTCT CACCCTGACC
    ATCTTGGATC CCCCAGCTCA TGGCACTCTG ATCAATGGCA TATATGGTCT TGAGATGAAT
    CGTTACAAAA GCATGAACCC AGAGGTTCTC CAACAAACCT TAGCAATCCA GTCCTTCACC
    TTGGAGGAGC TACAGCAAGG AATGAAGATC ATGTATATGC ACGATGACAC AGAAACCCTC
    AAGGACGCTT TCACCATACA GTTGACAGAT GGTGGGCACA CAGTCCAGGG GACTGCGTGT
    GTCCGCATCA TACCAGTCAA TGATGAAAAG CCATGGCTAC TAAAAAATGC AGGCGTTGAG
    GTCGAGGCCC TGGAGAAGAG GGTAATCTCC AGTGTAGTGC TGGAAGCAGG GGATCAGGAC
    ACTCCCAGTG ACCATCTTCT CTACATCCTC AACGCAGGTC CAAGATTTGG CCTTCTTCAG
    CTCAGGACAG AGGCTGGGTG GGTTGATCTG AGCCCAGGTC AGAATTTCAC CCAGGAGGAT
    GTGGAGATGA ATCGCCTGTG GTACTCTCAC ACTACAGTTA GTGGCTTCAA AGGTCACGAC
    AGGTTTCACT TTGTCCTCAC CGATGGGGAA AACACAACAC CTCCACAGAG TTTCTTCATC
    TCGGTCCGCA CAGTTCAGAA GGGCGACATT GCTCTCATCA CTAAGCCTGT GACCTTGCTG
    GAGGGAGAGA GAGTCATTCT GACCACTGAC ATTCTGACGG CCGCAGACAG TGGAGGCCGG
    CCAGATGAGC TCATATACAC TGTAGCAGTG CCACCTGAAC ATGGTCACCT GCACATGGTC
    CAAACGCCAG GAGTACCAGT GTTCTCCTTC TCCCAAATGG ATGTGGCTGC CAACCGGGTA
    TGCTACACTC ATGACAACAG CCGTTTTGCT GACCGTGATT CTTTCAGCTT TGCCATTAGT
    AACGGTGTAG CTTCCAGAAG TGGTACAGTT CATTTCACCA TTGAACACAG TGACCGGATC
    CCACCAACCC TGAGCACCAA CAAAGGCCTT GAGCTCACAG AGGGCTCTAT GAAGACCATC
    TCCACGGAAG AGCTAAAACT GACTGATCCA GATACAGCTC TAGAGAATCT CACCTATGTA
    GTTACTCAAA GCCCACAGTA TGGAAAGCTG CTCTTTAAAG GATTGCCTCT CTCCAAGCCT
    CGTTTCACAC AACTGGACAT CAACAATATG GATCTAGCTT ACCAACATCT GAATGGACGA
    GCCACGATCG ACAGGTTCGC CTTTCAGCCC ACTGATGGAA CGAACAAGGG CTATTTGGAG
    TACGGACAGC TAAAGAGTGA CCCGGCTGTT TTTACAATAC AGATAGAGAT ACTGGACAAG
    ACGCCACCCA GTATTATAAA CAAAGGCATC CCAAGTACTG TGGAGAACCT TCCGGATGGC
    AAACATGGCA TCTATATCAC TTCCAAAGAG CTGCAGGCCT CAGATCCAGA CAGTCCCGAC
    GACACACTCG AGTTCACCAT CACTAGACCT CCACACTTCG GCTTCCTCGA AAACACCCTC
    ACAGGTGCGT TTATCAAAGG ACGCTTCACT CAGAAGGATG TGGACCAGAA GGCTGTGCGC
    TATGTCATTC CTGTGGATGT GGAAGTGACG GCGGACAGCT TTGAATTTCA AGTCACAGAC
    CCAGCAGGAA ACACAATACT GCCTGAAGTG CTGGAGCTGC GCTGGTCTCG TGTGGATCTG
    TCAGCTTCCT GTTACCGTGT GTGTGAGACT GCAGGAACTC TGGCTGTGCA GGTTCTGCGC
    TCCGGAAACA GCAAAGATCC GGCTTACATC GGCATACAGG TGGAAGAAGG AACAGCCAAA
    GTCGGGAAAG ACTTCACTCA CAGCTCGGCC AGTCTGATAC AGTTCGATCC AGGTGTGAAT
    GTCAAAATGT GGAACATTTA CCTGAAAGAT GATGGACTGG AAGAGAACCA TGAGAGATTT
    GACATCATTC TGAAGGCTCC AAAAAATGCT GTTTTGGGAC AGAGGAACAA GGCCTCTGTT
    GAGATTGTGG ATCCTAGAAA TGGCAGGTGT AATCCAGATG ACCTTATTGT GGAGGAAGAT
    GAGAATCAAC ATACTTACCA AATCCAGCCA CACATCCCAG AGCCAGAAAC ACCCGTGCTT
    GAGGAGTACA CTCCGGACCC AAGAAGCGGG ATCCTCTGGG AGAACTATCC TCCAAGAGGA
    GACGTCCCGT ATCGCACTGA TTTTAACCAT TACAGCCTTA CAGGTCAACA TATGGAGCAG
    GAGGCCTTTC ACAGTCCAGG CCGGAGACAA CTGAGGGTCC TGGAAGGAAA CAGACAGAGA
    GCTGGTCTGT CAGAGGTGGA CAGCAGAAAT CAGGAAAGGG TTTGGAGATT TCACGGTGTG
    ATCCCGGTGA GCCAGCAGGA AGCGGCTCCT CGTGTTCACT CTGAACTGGA GATCACTCCC
    ATCTGGAGCT GGCCTGGAGA TTCAGCAGAA ACCCCTCAGC GTGACTCCGC CTCTGACATT
    CAACAACACA AATCTCAGAC ATCTGTTAGC ATCGATTGTC CAAATGGTTG GACTCACTAC
    CGGAGGAACT GCTATATTTT GGGGCCTGGT ACCGCTAGCT GGAGTAGCGC ACAACATGCC
    TGCACACTGC TTGAAGGCCA ATTGACAGGT ATCCACTCAA AGAATGATAT GAAGTGGATC
    TGGAAGTTTG CTGGGAAGCA GCCATTTTGG ATTGGACTGG TAGGTGGTCC GGAGCACGGA
    TGGATATGGA CGGATGGGAA AGTGCTGTCG TTCTCCAGAC TCAGGAAAGA TGAAGAGGAT
    CAAACTCGCT CTGTTTGTGT TTTAGCTCAG AGCCAGAAGA TGTGGATTCC TAAAGGTTGC
    ATTGATGGAT CCGAACACAG ATACGTTTGT TCAGCACCTG CACAAATCAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42552
    Clone ID Related Accession (Same CDS sequence) XM_009296245.3 , XM_009296245.2
    Accession Version XM_009296245.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product fras-related extracellular matrix protein 1b isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGGGTTT TCTTCCTCGC TCTAACAGTG CTCACCTCGA GTCACCTGTG GTCACATGTC 
    CAAGCTTCGA GTCTAGTGCA GTTAAATCGA GTTATTCGAG TAGCCAGAGG ACAATCTGTG
    TTCATCACAG CCAACGAACT TCAGTTCCAC ATGGATCACA AATCCGAGGC CTGTAAAGTA
    GAAGTGGTGC TTAATGAACC CATCACACAA AGAGTGGGAA AACTCACTCC ACAGGTTTTC
    GACTGCCAGT TCTTTCCAGA TGAGGTGAAG TATGTCCACA ATGGCAGTCC ACTTCTTGAG
    GAAGACACCG TCATGTTGAG AGTCTACAGG TTTACAGACA CCGAGACTTT TGTTGAGTCT
    ATGTTGCTGA AGGTACGTGT GTTTGAGCCT CAGAGAAGCC TTGTAGAGCT TGGAAATGTC
    CCTCTGGTGG TTCCTGAGTT CTATGGCCTT TCCAATGCCA TCAACGCCAG CGTTCTCACC
    TTTAAAACCC AGCCAGATGT GATCTGCACG CTTCGTCTTC TCAGCTCTGA GATCAGTGTT
    CCAGCACTGG GTCAGCTGGT GGTTGAGGAG TCTGGAACAA TGGTGGACAC TGAGGCTGGA
    CCAAGAAAAG GACGTCACAC TGCAATCGCC TGCCCTGGAA ATAAAGCCTG TGACCTCAAG
    ACAAGGCAAG TGGAGTTCCT GAAGACCGAC TGCAGTGAAT TCCTCTCTTC AGGTCTGAAG
    TACCAGCATC TCAGTCCTCC TTCACCTGAG ATTGACTACA TCCCAGTCCG GCTGGAGTTT
    CGAGACCAGA CCTCCCGAGC CCTGCTAGAG ACCGAGAGTG TCTGGTTACC GGTATTGATC
    CAGGGGGCGA TGCAGAACCA GCCGCCTCAC GCTGCCTTCA TGTCCACCTT CATCCTGGAG
    GTAGATCAGT TCATCCTGAC GCCTCTGAGC ACCGCTGCGC TGGATGCCAA AGACGACGAG
    ACACCGCAGG ACCAGCTCAT TTTCAGTGTC ACCAAACCTC CAGCCGAAGG ATATATAACT
    CATTTAGATG ATCACACTAA AATGGCCTCC TCCTTCTCAT GGCAAGACCT GAATGAGATG
    AAAATCGCAT ACCAGCCCCC AAACAGCAGT CATACAGCCA GGAGGAACTA TGAGGTTGAA
    TTTCAGGCCA TTGATGGCTC ATTTGCCCCG AGTCTTCCTA TCCTGATGCA CATCTCCATA
    CGCACTGCAG AGACAAACGC ACCTCGAGTC TCCTGGAATA TGGGCTTGGA TCTGCTGGAA
    GGCCAATCCA GACCAATCAC ATGGGAGCAT CTACAGATTG TAGATAAGGA CAATATTGAC
    GCCGTCACCT TGATCGCAAT GGACGGTCCA CTTAATGGAC ACTTAAGTGT TAGAGGTGGA
    AAAGGCTTCA TGTTTAAGGT CAAGGATCTC CGAGAGGGCG TTGTGGTCTA TCATCACTCG
    GACAGCGACA CCACCAGGGA TTACGTCGTC TTCCGAATCA CAGATGGCCG CCACAGCATC
    AGACATAAAT TCCCCATCTA CATCCTTCCC AAAGACGACA CGCCGCCTTT TTTAATCAAC
    AACGTGGCCT TCGAGGTGCA AGAAGGTGGC ACGGTTAGGG TGGAGGAGTA CATGCTGATG
    GCCTCAGATC TGGACTCCAG CGACGACTAC ATTCAGTATC AGCTGATTAC TTTTCCCAGA
    GCTGGACAGC TGATTAAGAA GTCTTCTCCA CATGAACCAG GTCTCCCAGT CAAGAGTTTC
    CTGCAGAGGG ACCTTTTCCA AGGGCTAATC TACTACAAGC ACTCGGGAGA GGAAGTGTTT
    GAGGACTCGT TTGATTTCAT CCTCTCAGAT GTCCATCAGC CTCCAAACCT GTCGGACAGA
    CATACTGTGG TGATCCACGT GTTTCCAGTG AAGGACCAGC TGCCTGTGGA AGTTTCTGGA
    ACGGTTCGCT CCATCACGGT TAAGGAAACG GAAGTTGTTT ACATCACTCA GAGCCTTCTG
    CACTTCAGAG ATACAGAGCA CCCGGATACT GACCTCATGT ATGTGATCAC CCACCCCTGC
    TTCAGTCCAG GAAACACCAG GTTATCAGAT GCTGGCAGGC TGTTTTATAC TGACAGCACA
    AATGCAATGA AAAAAGATGC TATGGTTCCA GTGCTGAAGT CCTTTACCCA GCATGCAGTG
    AACCACCACA AAGTGGCATA CATGCCTCCA ATAGAGGATA TTGGGCCTGA GCCTCTGTTT
    GTGCAGTTTG TGTTTTCTGT GAGTGACCAT CAGGGCGGCG CTCTTACAGG ACTCACCTTC
    AACATCACAG TCACGCCTGT TGACAACCAG GCCCCAGAAA TGTTTACTAA CTTGCTGAGG
    GTTGAGGAAG GTGGTGGTAG CTTCCTGATG GAGGAGCATC TTTTGGTTCA GGATGTGGAC
    AGCTCGGAAG ATCAGCTCAG GATACACATG AAGACCAAAC CCCAACATGG ACGGCTGGAG
    CTGCAGGGAA CGGCCATGCT AGAAGGAGAC AGCTTCACTC TGCGGGACCT CAAAGCATTG
    AGGGTCAGAT ACATTCATGA TGACTCTGAG ACCCAAAAGG ATAGCATTGG ACTTACAATC
    ACAGACGGAA TCAACTCAGC ACATGGAGCA CTTTTAATTC AGATCCTGCC TGTGAATGAT
    GAACCGCCAC AGTTGGGCTC TGATTTGAAG GCAAAGCTGT CCTGCAAAGA GGGGGGTCAG
    GTTCAGATTA CGGTCGAGTA CCTCTCTGCT ACAGATGTTG ACAGCGATGA CACTCGGCTA
    ACATACATGC TTGCCAGAAC ACCTGGGCGT GGGGTCATAC AGCGAAACGG GGTCACTGTG
    GACAAGTTCT CCCAGCTGGA TGTGCTCAAT GGACTCATCT TCTACCTGCA CACAGGTGGT
    GAGATTGGAC CGGATCCTGT CTCGGACACT GTCACCCTCA TTGTGTCAGA TGGAGAGGCT
    GGAACAATGG ACGGCTGCTG CCAGGAGGAC GCTCTCCCCC CTCCCGTCCC CCTGCATGGC
    ACGCTTCCGG TCTATGACCT GAACATCACT GTGATGCCTG TCAACAACCA GGTCCCAACC
    ATTACGCTGG GCGGCATGAT TGTGGTAGAT GAAGGTGCCA GAGCATGTCT GTGTGGGGGG
    GTGTTGGAGG CCAGCGATCC TGATAGTCGT CCAGAAGAAC TCATGTTTCA CTTGGTCACC
    CCGCCTCAGT ATGGGTTCCT GGAGAACACC CTTCCATCCC CTGGCTTCGA GAAAAGCAAT
    GCTGGACTGA GAGTGGTTTT TTTCAGCCAG CTCCACCTCA GTTCGGGTTT CATCAACTAT
    GTCCAGTCGG TGCACCAGGG TGTGGAGCCT ACAGCAGACT TCTTTACCAT CAGTGTCAGT
    GACGGAATGC AAAGATCTGC ACCATTGCAC GTCTACATCA TCATCAACCC CACCAATGAT
    GAGGTTCCAT CACTGCTGCT TAGCAACTTC ACAGTCATGG AGGGAGGAAT GAAGGATCTC
    AGCCCTGATA TTCTAAACGC AGTGGACGTT GATATCCCAG CAGAAAGTCT CACCCTGACC
    ATCTTGGATC CCCCAGCTCA TGGCACTCTG ATCAATGGCA TATATGGTCT TGAGATGAAT
    CGTTACAAAA GCATGAACCC AGAGGTTCTC CAACAAACCT TAGCAATCCA GTCCTTCACC
    TTGGAGGAGC TACAGCAAGG AATGAAGATC ATGTATATGC ACGATGACAC AGAAACCCTC
    AAGGACGCTT TCACCATACA GTTGACAGAT GGTGGGCACA CAGTCCAGGG GACTGCGTGT
    GTCCGCATCA TACCAGTCAA TGATGAAAAG CCATGGCTAC TAAAAAATGC AGGCGTTGAG
    GTCGAGGCCC TGGAGAAGAG GGTAATCTCC AGTGTAGTGC TGGAAGCAGG GGATCAGGAC
    ACTCCCAGTG ACCATCTTCT CTACATCCTC AACGCAGGTC CAAGATTTGG CCTTCTTCAG
    CTCAGGACAG AGGCTGGGTG GGTTGATCTG AGCCCAGGTC AGAATTTCAC CCAGGAGGAT
    GTGGAGATGA ATCGCCTGTG GTACTCTCAC ACTACAGTTA GTGGCTTCAA AGGTCACGAC
    AGGTTTCACT TTGTCCTCAC CGATGGGGAA AACACAACAC CTCCACAGAG TTTCTTCATC
    TCGGTCCGCA CAGTTCAGAA GGGCGACATT GCTCTCATCA CTAAGCCTGT GACCTTGCTG
    GAGGGAGAGA GAGTCATTCT GACCACTGAC ATTCTGACGG CCGCAGACAG TGGAGGCCGG
    CCAGATGAGC TCATATACAC TGTAGCAGTG CCACCTGAAC ATGGTCACCT GCACATGGTC
    CAAACGCCAG GAGTACCAGT GTTCTCCTTC TCCCAAATGG ATGTGGCTGC CAACCGGGTA
    TGCTACACTC ATGACAACAG CCGTTTTGCT GACCGTGATT CTTTCAGCTT TGCCATTAGT
    AACGGTGTAG CTTCCAGAAG TGGTACAGTT CATTTCACCA TTGAACACAG TGACCGGATC
    CCACCAACCC TGAGCACCAA CAAAGGCCTT GAGCTCACAG AGGGCTCTAT GAAGACCATC
    TCCACGGAAG AGCTAAAACT GACTGATCCA GATACAGCTC TAGAGAATCT CACCTATGTA
    GTTACTCAAA GCCCACAGTA TGGAAAGCTG CTCTTTAAAG GATTGCCTCT CTCCAAGCCT
    CGTTTCACAC AACTGGACAT CAACAATATG GATCTAGCTT ACCAACATCT GAATGGACGA
    GCCACGATCG ACAGGTTCGC CTTTCAGCCC ACTGATGGAA CGAACAAGGG CTATTTGGAG
    TACGGACAGC TAAAGAGTGA CCCGGCTGTT TTTACAATAC AGATAGAGAT ACTGGACAAG
    ACGCCACCCA GTATTATAAA CAAAGGCATC CCAAGTACTG TGGAGAACCT TCCGGATGGC
    AAACATGGCA TCTATATCAC TTCCAAAGAG CTGCAGGCCT CAGATCCAGA CAGTCCCGAC
    GACACACTCG AGTTCACCAT CACTAGACCT CCACACTTCG GCTTCCTCGA AAACACCCTC
    ACAGGTGCGT TTATCAAAGG ACGCTTCACT CAGAAGGATG TGGACCAGAA GGCTGTGCGC
    TATGTCATTC CTGTGGATGT GGAAGTGACG GCGGACAGCT TTGAATTTCA AGTCACAGAC
    CCAGCAGGAA ACACAATACT GCCTGAAGTG CTGGAGCTGC GCTGGTCTCG TGTGGATCTG
    TCAGCTTCCT GTTACCGTGT GTGTGAGACT GCAGGAACTC TGGCTGTGCA GGTTCTGCGC
    TCCGGAAACA GCAAAGATCC GGCTTACATC GGCATACAGG TGGAAGAAGG AACAGCCAAA
    GTCGGGAAAG ACTTCACTCA CAGCTCGGCC AGTCTGATAC AGTTCGATCC AGGTGTGAAT
    GTCAAAATGT GGAACATTTA CCTGAAAGAT GATGGACTGG AAGAGAACCA TGAGAGATTT
    GACATCATTC TGAAGGCTCC AAAAAATGCT GTTTTGGGAC AGAGGAACAA GGCCTCTGTT
    GAGATTGTGG ATCCTAGAAA TGGCAGGTGT AATCCAGATG ACCTTATTGT GGAGGAAGAT
    GAGAATCAAC ATACTTACCA AATCCAGCCA CACATCCCAG AGCCAGAAAC ACCCGTGCTT
    GAGGAGTACA CTCCGGACCC AAGAAGCGGG ATCCTCTGGG AGAACTATCC TCCAAGAGGA
    GACGTCCCGT ATCGCACTGA TTTTAACCAT TACAGCCTTA CAGGTCAACA TATGGAGCAG
    GAGGCCTTTC ACAGTCCAGG CCGGAGACAA CTGAGGGTCC TGGAAGGAAA CAGACAGAGA
    GCTGGTCTGT CAGAGGTGGA CAGCAGAAAT CAGGAAAGGG TTTGGAGATT TCACGGTGTG
    ATCCCGGTGA GCCAGCAGGA AGCGGCTCCT CGTGTTCACT CTGAACTGGA GATCACTCCC
    ATCTGGAGCT GGCCTGGAGA TTCAGCAGAA ACCCCTCAGC GTGACTCCGC CTCTGACATT
    CAACAACACA AATCTCAGAC ATCTGTTAGC ATCGATTGTC CAAATGGTTG GACTCACTAC
    CGGAGGAACT GCTATATTTT GGGGCCTGGT ACCGCTAGCT GGAGTAGCGC ACAACATGCC
    TGCACACTGC TTGAAGGCCA ATTGACAGGT ATCCACTCAA AGAATGATAT GAAGTGGATC
    TGGAAGTTTG CTGGGAAGCA GCCATTTTGG ATTGGACTGG TAGGTGGTCC GGAGCACGGA
    TGGATATGGA CGGATGGGAA AGTGCTGTCG TTCTCCAGAC TCAGGAAAGA TGAAGAGGAT
    CAAACTCGCT CTGTTTGTGT TTTAGCTCAG AGCCAGAAGA TGTGGATTCC TAAAGGTTGC
    ATTGATGGAT CCGAACACAG ATACGTTTGT TCAGCACCTG CACAAATCAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009294520.1
    CDS474..6887
    Translation

    Target ORF information:

    RefSeq Version XM_009296245.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Fras1 related extracellular matrix 1b (frem1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGGGTTT TCTTCCTCGC TCTAACAGTG CTCACCTCGA GTCACCTGTG GTCACATGTC 
    CAAGCTTCGA GTCTAGTGCA GTTAAATCGA GTTATTCGAG TAGCCAGAGG ACAATCTGTG
    TTCATCACAG CCAACGAACT TCAGTTCCAC ATGGATCACA AATCCGAGGC CTGTAAAGTA
    GAAGTGGTGC TTAATGAACC CATCACACAA AGAGTGGGAA AACTCACTCC ACAGGTTTTC
    GACTGCCAGT TCTTTCCAGA TGAGGTGAAG TATGTCCACA ATGGCAGTCC ACTTCTTGAG
    GAAGACACCG TCATGTTGAG AGTCTACAGG TTTACAGACA CCGAGACTTT TGTTGAGTCT
    ATGTTGCTGA AGGTACGTGT GTTTGAGCCT CAGAGAAGCC TTGTAGAGCT TGGAAATGTC
    CCTCTGGTGG TTCCTGAGTT CTATGGCCTT TCCAATGCCA TCAACGCCAG CGTTCTCACC
    TTTAAAACCC AGCCAGATGT GATCTGCACG CTTCGTCTTC TCAGCTCTGA GATCAGTGTT
    CCAGCACTGG GTCAGCTGGT GGTTGAGGAG TCTGGAACAA TGGTGGACAC TGAGGCTGGA
    CCAAGAAAAG GACGTCACAC TGCAATCGCC TGCCCTGGAA ATAAAGCCTG TGACCTCAAG
    ACAAGGCAAG TGGAGTTCCT GAAGACCGAC TGCAGTGAAT TCCTCTCTTC AGGTCTGAAG
    TACCAGCATC TCAGTCCTCC TTCACCTGAG ATTGACTACA TCCCAGTCCG GCTGGAGTTT
    CGAGACCAGA CCTCCCGAGC CCTGCTAGAG ACCGAGAGTG TCTGGTTACC GGTATTGATC
    CAGGGGGCGA TGCAGAACCA GCCGCCTCAC GCTGCCTTCA TGTCCACCTT CATCCTGGAG
    GTAGATCAGT TCATCCTGAC GCCTCTGAGC ACCGCTGCGC TGGATGCCAA AGACGACGAG
    ACACCGCAGG ACCAGCTCAT TTTCAGTGTC ACCAAACCTC CAGCCGAAGG ATATATAACT
    CATTTAGATG ATCACACTAA AATGGCCTCC TCCTTCTCAT GGCAAGACCT GAATGAGATG
    AAAATCGCAT ACCAGCCCCC AAACAGCAGT CATACAGCCA GGAGGAACTA TGAGGTTGAA
    TTTCAGGCCA TTGATGGCTC ATTTGCCCCG AGTCTTCCTA TCCTGATGCA CATCTCCATA
    CGCACTGCAG AGACAAACGC ACCTCGAGTC TCCTGGAATA TGGGCTTGGA TCTGCTGGAA
    GGCCAATCCA GACCAATCAC ATGGGAGCAT CTACAGATTG TAGATAAGGA CAATATTGAC
    GCCGTCACCT TGATCGCAAT GGACGGTCCA CTTAATGGAC ACTTAAGTGT TAGAGGTGGA
    AAAGGCTTCA TGTTTAAGGT CAAGGATCTC CGAGAGGGCG TTGTGGTCTA TCATCACTCG
    GACAGCGACA CCACCAGGGA TTACGTCGTC TTCCGAATCA CAGATGGCCG CCACAGCATC
    AGACATAAAT TCCCCATCTA CATCCTTCCC AAAGACGACA CGCCGCCTTT TTTAATCAAC
    AACGTGGCCT TCGAGGTGCA AGAAGGTGGC ACGGTTAGGG TGGAGGAGTA CATGCTGATG
    GCCTCAGATC TGGACTCCAG CGACGACTAC ATTCAGTATC AGCTGATTAC TTTTCCCAGA
    GCTGGACAGC TGATTAAGAA GTCTTCTCCA CATGAACCAG GTCTCCCAGT CAAGAGTTTC
    CTGCAGAGGG ACCTTTTCCA AGGGCTAATC TACTACAAGC ACTCGGGAGA GGAAGTGTTT
    GAGGACTCGT TTGATTTCAT CCTCTCAGAT GTCCATCAGC CTCCAAACCT GTCGGACAGA
    CATACTGTGG TGATCCACGT GTTTCCAGTG AAGGACCAGC TGCCTGTGGA AGTTTCTGGA
    ACGGTTCGCT CCATCACGGT TAAGGAAACG GAAGTTGTTT ACATCACTCA GAGCCTTCTG
    CACTTCAGAG ATACAGAGCA CCCGGATACT GACCTCATGT ATGTGATCAC CCACCCCTGC
    TTCAGTCCAG GAAACACCAG GTTATCAGAT GCTGGCAGGC TGTTTTATAC TGACAGCACA
    AATGCAATGA AAAAAGATGC TATGGTTCCA GTGCTGAAGT CCTTTACCCA GCATGCAGTG
    AACCACCACA AAGTGGCATA CATGCCTCCA ATAGAGGATA TTGGGCCTGA GCCTCTGTTT
    GTGCAGTTTG TGTTTTCTGT GAGTGACCAT CAGGGCGGCG CTCTTACAGG ACTCACCTTC
    AACATCACAG TCACGCCTGT TGACAACCAG GCCCCAGAAA TGTTTACTAA CTTGCTGAGG
    GTTGAGGAAG GTGGTGGTAG CTTCCTGATG GAGGAGCATC TTTTGGTTCA GGATGTGGAC
    AGCTCGGAAG ATCAGCTCAG GATACACATG AAGACCAAAC CCCAACATGG ACGGCTGGAG
    CTGCAGGGAA CGGCCATGCT AGAAGGAGAC AGCTTCACTC TGCGGGACCT CAAAGCATTG
    AGGGTCAGAT ACATTCATGA TGACTCTGAG ACCCAAAAGG ATAGCATTGG ACTTACAATC
    ACAGACGGAA TCAACTCAGC ACATGGAGCA CTTTTAATTC AGATCCTGCC TGTGAATGAT
    GAACCGCCAC AGTTGGGCTC TGATTTGAAG GCAAAGCTGT CCTGCAAAGA GGGGGGTCAG
    GTTCAGATTA CGGTCGAGTA CCTCTCTGCT ACAGATGTTG ACAGCGATGA CACTCGGCTA
    ACATACATGC TTGCCAGAAC ACCTGGGCGT GGGGTCATAC AGCGAAACGG GGTCACTGTG
    GACAAGTTCT CCCAGCTGGA TGTGCTCAAT GGACTCATCT TCTACCTGCA CACAGGTGGT
    GAGATTGGAC CGGATCCTGT CTCGGACACT GTCACCCTCA TTGTGTCAGA TGGAGAGGCT
    GGAACAATGG ACGGCTGCTG CCAGGAGGAC GCTCTCCCCC CTCCCGTCCC CCTGCATGGC
    ACGCTTCCGG TCTATGACCT GAACATCACT GTGATGCCTG TCAACAACCA GGTCCCAACC
    ATTACGCTGG GCGGCATGAT TGTGGTAGAT GAAGGTGCCA GAGCATGTCT GTGTGGGGGG
    GTGTTGGAGG CCAGCGATCC TGATAGTCGT CCAGAAGAAC TCATGTTTCA CTTGGTCACC
    CCGCCTCAGT ATGGGTTCCT GGAGAACACC CTTCCATCCC CTGGCTTCGA GAAAAGCAAT
    GCTGGACTGA GAGTGGTTTT TTTCAGCCAG CTCCACCTCA GTTCGGGTTT CATCAACTAT
    GTCCAGTCGG TGCACCAGGG TGTGGAGCCT ACAGCAGACT TCTTTACCAT CAGTGTCAGT
    GACGGAATGC AAAGATCTGC ACCATTGCAC GTCTACATCA TCATCAACCC CACCAATGAT
    GAGGTTCCAT CACTGCTGCT TAGCAACTTC ACAGTCATGG AGGGAGGAAT GAAGGATCTC
    AGCCCTGATA TTCTAAACGC AGTGGACGTT GATATCCCAG CAGAAAGTCT CACCCTGACC
    ATCTTGGATC CCCCAGCTCA TGGCACTCTG ATCAATGGCA TATATGGTCT TGAGATGAAT
    CGTTACAAAA GCATGAACCC AGAGGTTCTC CAACAAACCT TAGCAATCCA GTCCTTCACC
    TTGGAGGAGC TACAGCAAGG AATGAAGATC ATGTATATGC ACGATGACAC AGAAACCCTC
    AAGGACGCTT TCACCATACA GTTGACAGAT GGTGGGCACA CAGTCCAGGG GACTGCGTGT
    GTCCGCATCA TACCAGTCAA TGATGAAAAG CCATGGCTAC TAAAAAATGC AGGCGTTGAG
    GTCGAGGCCC TGGAGAAGAG GGTAATCTCC AGTGTAGTGC TGGAAGCAGG GGATCAGGAC
    ACTCCCAGTG ACCATCTTCT CTACATCCTC AACGCAGGTC CAAGATTTGG CCTTCTTCAG
    CTCAGGACAG AGGCTGGGTG GGTTGATCTG AGCCCAGGTC AGAATTTCAC CCAGGAGGAT
    GTGGAGATGA ATCGCCTGTG GTACTCTCAC ACTACAGTTA GTGGCTTCAA AGGTCACGAC
    AGGTTTCACT TTGTCCTCAC CGATGGGGAA AACACAACAC CTCCACAGAG TTTCTTCATC
    TCGGTCCGCA CAGTTCAGAA GGGCGACATT GCTCTCATCA CTAAGCCTGT GACCTTGCTG
    GAGGGAGAGA GAGTCATTCT GACCACTGAC ATTCTGACGG CCGCAGACAG TGGAGGCCGG
    CCAGATGAGC TCATATACAC TGTAGCAGTG CCACCTGAAC ATGGTCACCT GCACATGGTC
    CAAACGCCAG GAGTACCAGT GTTCTCCTTC TCCCAAATGG ATGTGGCTGC CAACCGGGTA
    TGCTACACTC ATGACAACAG CCGTTTTGCT GACCGTGATT CTTTCAGCTT TGCCATTAGT
    AACGGTGTAG CTTCCAGAAG TGGTACAGTT CATTTCACCA TTGAACACAG TGACCGGATC
    CCACCAACCC TGAGCACCAA CAAAGGCCTT GAGCTCACAG AGGGCTCTAT GAAGACCATC
    TCCACGGAAG AGCTAAAACT GACTGATCCA GATACAGCTC TAGAGAATCT CACCTATGTA
    GTTACTCAAA GCCCACAGTA TGGAAAGCTG CTCTTTAAAG GATTGCCTCT CTCCAAGCCT
    CGTTTCACAC AACTGGACAT CAACAATATG GATCTAGCTT ACCAACATCT GAATGGACGA
    GCCACGATCG ACAGGTTCGC CTTTCAGCCC ACTGATGGAA CGAACAAGGG CTATTTGGAG
    TACGGACAGC TAAAGAGTGA CCCGGCTGTT TTTACAATAC AGATAGAGAT ACTGGACAAG
    ACGCCACCCA GTATTATAAA CAAAGGCATC CCAAGTACTG TGGAGAACCT TCCGGATGGC
    AAACATGGCA TCTATATCAC TTCCAAAGAG CTGCAGGCCT CAGATCCAGA CAGTCCCGAC
    GACACACTCG AGTTCACCAT CACTAGACCT CCACACTTCG GCTTCCTCGA AAACACCCTC
    ACAGGTGCGT TTATCAAAGG ACGCTTCACT CAGAAGGATG TGGACCAGAA GGCTGTGCGC
    TATGTCATTC CTGTGGATGT GGAAGTGACG GCGGACAGCT TTGAATTTCA AGTCACAGAC
    CCAGCAGGAA ACACAATACT GCCTGAAGTG CTGGAGCTGC GCTGGTCTCG TGTGGATCTG
    TCAGCTTCCT GTTACCGTGT GTGTGAGACT GCAGGAACTC TGGCTGTGCA GGTTCTGCGC
    TCCGGAAACA GCAAAGATCC GGCTTACATC GGCATACAGG TGGAAGAAGG AACAGCCAAA
    GTCGGGAAAG ACTTCACTCA CAGCTCGGCC AGTCTGATAC AGTTCGATCC AGGTGTGAAT
    GTCAAAATGT GGAACATTTA CCTGAAAGAT GATGGACTGG AAGAGAACCA TGAGAGATTT
    GACATCATTC TGAAGGCTCC AAAAAATGCT GTTTTGGGAC AGAGGAACAA GGCCTCTGTT
    GAGATTGTGG ATCCTAGAAA TGGCAGGTGT AATCCAGATG ACCTTATTGT GGAGGAAGAT
    GAGAATCAAC ATACTTACCA AATCCAGCCA CACATCCCAG AGCCAGAAAC ACCCGTGCTT
    GAGGAGTACA CTCCGGACCC AAGAAGCGGG ATCCTCTGGG AGAACTATCC TCCAAGAGGA
    GACGTCCCGT ATCGCACTGA TTTTAACCAT TACAGCCTTA CAGGTCAACA TATGGAGCAG
    GAGGCCTTTC ACAGTCCAGG CCGGAGACAA CTGAGGGTCC TGGAAGGAAA CAGACAGAGA
    GCTGGTCTGT CAGAGGTGGA CAGCAGAAAT CAGGAAAGGG TTTGGAGATT TCACGGTGTG
    ATCCCGGTGA GCCAGCAGGA AGCGGCTCCT CGTGTTCACT CTGAACTGGA GATCACTCCC
    ATCTGGAGCT GGCCTGGAGA TTCAGCAGAA ACCCCTCAGC GTGACTCCGC CTCTGACATT
    CAACAACACA AATCTCAGAC ATCTGTTAGC ATCGATTGTC CAAATGGTTG GACTCACTAC
    CGGAGGAACT GCTATATTTT GGGGCCTGGT ACCGCTAGCT GGAGTAGCGC ACAACATGCC
    TGCACACTGC TTGAAGGCCA ATTGACAGGT ATCCACTCAA AGAATGATAT GAAGTGGATC
    TGGAAGTTTG CTGGGAAGCA GCCATTTTGG ATTGGACTGG TAGGTGGTCC GGAGCACGGA
    TGGATATGGA CGGATGGGAA AGTGCTGTCG TTCTCCAGAC TCAGGAAAGA TGAAGAGGAT
    CAAACTCGCT CTGTTTGTGT TTTAGCTCAG AGCCAGAAGA TGTGGATTCC TAAAGGTTGC
    ATTGATGGAT CCGAACACAG ATACGTTTGT TCAGCACCTG CACAAATCAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.