HFM1 cDNA ORF clone, Danio rerio(Zebrafish)

The following HFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa42494 XM_009298503.1
Latest version!
Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa56422 XM_017351616.1
Latest version!
Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa42490 XM_021467172.1
Latest version!
Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa56421 XM_017351609.1
Latest version!
Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa42492 XM_009298501.3
Latest version!
Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa42493 XM_009298502.2
Latest version!
Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa42493 XM_009298502.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa42492 XM_009298501.2 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa42494
    Clone ID Related Accession (Same CDS sequence) XM_009298503.1
    Accession Version XM_009298503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGGTTTCCA ATAGAAGGGA AGATCAAAAG
    CAACGACATG AAAGTGAATT GTCTGATACA AGCTCAGCTT GGCTGCATCC CCATTCAGGA
    GTTTGGACTC ATACAGGATA CTGGAAAAAT ATTCAGAAAT GCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296778.1
    CDS211..2658
    Translation

    Target ORF information:

    RefSeq Version XM_009298503.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGGTTTCCA ATAGAAGGGA AGATCAAAAG
    CAACGACATG AAAGTGAATT GTCTGATACA AGCTCAGCTT GGCTGCATCC CCATTCAGGA
    GTTTGGACTC ATACAGGATA CTGGAAAAAT ATTCAGAAAT GCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56422
    Clone ID Related Accession (Same CDS sequence) XM_017351616.1
    Accession Version XM_017351616.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable ATP-dependent DNA helicase HFM1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGGTTTCCA ATAGAAGGGA AGATCAAAAG
    CAACGACATG AAAGTGAATT GGTGAAGTCT TGCTTTTGCT GTTTGATTGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207105.1
    CDS211..2604
    Translation

    Target ORF information:

    RefSeq Version XM_017351616.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351616.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGGTTTCCA ATAGAAGGGA AGATCAAAAG
    CAACGACATG AAAGTGAATT GGTGAAGTCT TGCTTTTGCT GTTTGATTGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42490
    Clone ID Related Accession (Same CDS sequence) XM_021467172.1
    Accession Version XM_021467172.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG
    CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT
    CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC
    GACATTCTCT TCGTGGATCA AGTTGCTCCA AACATGGTTA AGAGAAATTG CCATCATCTC
    TGCAAAAACA AAGAGCTCTG TAGACATGAC TGCTGCAAAA TTGGAGTCAA AAGGACTGTA
    CTACAGTCAA ATGTCTCCTC ATACCTTGAC AACCTAAAAA TGAGGAATGA GAAACTTTGT
    GAGACTCCTG TGAAGCGATT GAAGATGATG ATGAATAATG GCACAGCAGC TGTAAGCATG
    CAGCAGTTCT CCTACAAACC CAAAGACACA CATTCTGCTG CCAAGAGGTT TGAAGGAAGT
    CAGTGTTACA CTCAGAGTGC TTATCAAATC CAGGATCTAG AGGCCAGTTT TGCCACTCCA
    TCCCAGAATT CATCATCTGA GGCATGGCAT GATGGTTACA TTGATGAGCT GGGAGATGGA
    TATACTGAAC ATGTAAACCC GGCATTAGAA CATCCTCAAC TGTCTGATTT ACCTCAAAAA
    GGTGAGCAAA GTTCTCTCGG ATTGGAAGGA CATGAGGAGA TGACATTTGA CAGTCAGCCT
    AAGCCACACA GTGATGGAAA CATGCCCAGG ATCACTCTTA ATAAAGCAAG AGTTTATTCA
    GACTTAAACG ACAGCACCGA CAGGACAGGT CTCAGGTCTG ATTTTCCTGA TGTGACCTTT
    GACCTGGGAG TAAACTATTG GGATGATTTT GATGACAGAA ACCTGGTTGA TGCAAGTAAT
    GTATGCTGTG AATCCCAACA ATTTAACCCG ATGATAGAGC ACCATCAGCC TGCACATTCT
    GGTTTGTCCA GTGGTTTGTC TCATTGTCCA GCACAAACGT TGCAAGAAAC TGTGAGTCCT
    GTCTTACCAC AAAGGGTGCA ACCAATGGCC GTTACTCCTC AAACTGCAAT GTGGACAAAC
    TTGAACACAA ACACCCTCTC AAGTGACCAG AGTCAAGCCA GTAAAGGTTC AGTCCAAAAA
    AGATTTGATT TTTTCTCAAG GACAGATGCA GCTTTAAAGG AAAACACACC TGTTGGCAGT
    GATCACACGA AGGAAATCAG CGATGAAGCC CATGTTTTTT TTGGTATGTT TGATGGCCTT
    TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322847.1
    CDS452..4837
    Translation

    Target ORF information:

    RefSeq Version XM_021467172.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG
    CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT
    CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC
    GACATTCTCT TCGTGGATCA AGTTGCTCCA AACATGGTTA AGAGAAATTG CCATCATCTC
    TGCAAAAACA AAGAGCTCTG TAGACATGAC TGCTGCAAAA TTGGAGTCAA AAGGACTGTA
    CTACAGTCAA ATGTCTCCTC ATACCTTGAC AACCTAAAAA TGAGGAATGA GAAACTTTGT
    GAGACTCCTG TGAAGCGATT GAAGATGATG ATGAATAATG GCACAGCAGC TGTAAGCATG
    CAGCAGTTCT CCTACAAACC CAAAGACACA CATTCTGCTG CCAAGAGGTT TGAAGGAAGT
    CAGTGTTACA CTCAGAGTGC TTATCAAATC CAGGATCTAG AGGCCAGTTT TGCCACTCCA
    TCCCAGAATT CATCATCTGA GGCATGGCAT GATGGTTACA TTGATGAGCT GGGAGATGGA
    TATACTGAAC ATGTAAACCC GGCATTAGAA CATCCTCAAC TGTCTGATTT ACCTCAAAAA
    GGTGAGCAAA GTTCTCTCGG ATTGGAAGGA CATGAGGAGA TGACATTTGA CAGTCAGCCT
    AAGCCACACA GTGATGGAAA CATGCCCAGG ATCACTCTTA ATAAAGCAAG AGTTTATTCA
    GACTTAAACG ACAGCACCGA CAGGACAGGT CTCAGGTCTG ATTTTCCTGA TGTGACCTTT
    GACCTGGGAG TAAACTATTG GGATGATTTT GATGACAGAA ACCTGGTTGA TGCAAGTAAT
    GTATGCTGTG AATCCCAACA ATTTAACCCG ATGATAGAGC ACCATCAGCC TGCACATTCT
    GGTTTGTCCA GTGGTTTGTC TCATTGTCCA GCACAAACGT TGCAAGAAAC TGTGAGTCCT
    GTCTTACCAC AAAGGGTGCA ACCAATGGCC GTTACTCCTC AAACTGCAAT GTGGACAAAC
    TTGAACACAA ACACCCTCTC AAGTGACCAG AGTCAAGCCA GTAAAGGTTC AGTCCAAAAA
    AGATTTGATT TTTTCTCAAG GACAGATGCA GCTTTAAAGG AAAACACACC TGTTGGCAGT
    GATCACACGA AGGAAATCAG CGATGAAGCC CATGTTTTTT TTGGTATGTT TGATGGCCTT
    TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56421
    Clone ID Related Accession (Same CDS sequence) XM_017351609.1
    Accession Version XM_017351609.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG
    CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT
    CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC
    GACATTCTCT TCGTGGATCA AGTTGCTCCA AACATGGTTA AGAGAAATTG CCATCATCTC
    TGCAAAAACA AAGAGCTCTG TAGACATGAC TGCTGCAAAA TTGGAGTCAA AAGGACTGTA
    CTACAGTCAA ATGTCTCCTC ATACCTTGAC AACCTAAAAA TGAGGAATGA GAAACTTTGT
    GAGACTCCTG TGAAGCGATT GAAGATGATG ATGAATAATG GCACAGCAGC TGTAAGCATG
    CAGCAGTTCT CCTACAAACC CAAAGACACA CATTCTGCTG CCAAGAGGTT TGAAGGAAGT
    CAGTGTTACA CTCAGAGTGC TTATCAAATC CAGGATCTAG AGGCCAGTTT TGCCACTCCA
    TCCCAGAATT CATCATCTGA GGCATGGCAT GATGGTTACA TTGATGAGCT GGGAGATGGA
    TATACTGAAC ATGTAAACCC GGCATTAGAA CATCCTCAAC TGTCTGATTT ACCTCAAAAG
    ATGACATTTG ACAGTCAGCC TAAGCCACAC AGTGATGGAA ACATGCCCAG GATCACTCTT
    AATAAAGCAA GAGTTTATTC AGACTTAAAC GACAGCACCG ACAGGACAGG TCTCAGGTCT
    GATTTTCCTG ATGTGACCTT TGACCTGGGA GTAAACTATT GGGATGATTT TGATGACAGA
    AACCTGGTTG ATGCAAGTAA TGTATGCTGT GAATCCCAAC AATTTAACCC GATGATAGAG
    CACCATCAGC CTGCACATTC TGGTTTGTCC AGTGGTTTGT CTCATTGTCC AGCACAAACG
    TTGCAAGAAA CTGTGAGTCC TGTCTTACCA CAAAGGGTGC AACCAATGGC CGTTACTCCT
    CAAACTGCAA TGTGGACAAA CTTGAACACA AACACCCTCT CAAGTGACCA GAGTCAAGCC
    AGTAAAGGTT CAGTCCAAAA AAGATTTGAT TTTTTCTCAA GGACAGATGC AGCTTTAAAG
    GAAAACACAC CTGTTGGCAG TGATCACACG AAGGAAATCA GCGATGAAGC CCATGTTTTT
    TTTGGTATGT TTGATGGCCT TTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207098.1
    CDS211..4557
    Translation

    Target ORF information:

    RefSeq Version XM_017351609.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351609.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG
    CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT
    CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC
    GACATTCTCT TCGTGGATCA AGTTGCTCCA AACATGGTTA AGAGAAATTG CCATCATCTC
    TGCAAAAACA AAGAGCTCTG TAGACATGAC TGCTGCAAAA TTGGAGTCAA AAGGACTGTA
    CTACAGTCAA ATGTCTCCTC ATACCTTGAC AACCTAAAAA TGAGGAATGA GAAACTTTGT
    GAGACTCCTG TGAAGCGATT GAAGATGATG ATGAATAATG GCACAGCAGC TGTAAGCATG
    CAGCAGTTCT CCTACAAACC CAAAGACACA CATTCTGCTG CCAAGAGGTT TGAAGGAAGT
    CAGTGTTACA CTCAGAGTGC TTATCAAATC CAGGATCTAG AGGCCAGTTT TGCCACTCCA
    TCCCAGAATT CATCATCTGA GGCATGGCAT GATGGTTACA TTGATGAGCT GGGAGATGGA
    TATACTGAAC ATGTAAACCC GGCATTAGAA CATCCTCAAC TGTCTGATTT ACCTCAAAAG
    ATGACATTTG ACAGTCAGCC TAAGCCACAC AGTGATGGAA ACATGCCCAG GATCACTCTT
    AATAAAGCAA GAGTTTATTC AGACTTAAAC GACAGCACCG ACAGGACAGG TCTCAGGTCT
    GATTTTCCTG ATGTGACCTT TGACCTGGGA GTAAACTATT GGGATGATTT TGATGACAGA
    AACCTGGTTG ATGCAAGTAA TGTATGCTGT GAATCCCAAC AATTTAACCC GATGATAGAG
    CACCATCAGC CTGCACATTC TGGTTTGTCC AGTGGTTTGT CTCATTGTCC AGCACAAACG
    TTGCAAGAAA CTGTGAGTCC TGTCTTACCA CAAAGGGTGC AACCAATGGC CGTTACTCCT
    CAAACTGCAA TGTGGACAAA CTTGAACACA AACACCCTCT CAAGTGACCA GAGTCAAGCC
    AGTAAAGGTT CAGTCCAAAA AAGATTTGAT TTTTTCTCAA GGACAGATGC AGCTTTAAAG
    GAAAACACAC CTGTTGGCAG TGATCACACG AAGGAAATCA GCGATGAAGC CCATGTTTTT
    TTTGGTATGT TTGATGGCCT TTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42492
    Clone ID Related Accession (Same CDS sequence) XM_009298501.3 , XM_009298501.2
    Accession Version XM_009298501.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG
    CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT
    CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC
    GGTCAGACTC AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296776.1
    CDS452..3766
    Translation

    Target ORF information:

    RefSeq Version XM_009298501.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298501.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG
    CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT
    CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC
    GGTCAGACTC AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42492
    Clone ID Related Accession (Same CDS sequence) XM_009298501.3 , XM_009298501.2
    Accession Version XM_009298501.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG
    CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT
    CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC
    GGTCAGACTC AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296776.1
    CDS211..3525
    Translation

    Target ORF information:

    RefSeq Version XM_009298501.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298501.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG
    CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT
    CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC
    GGTCAGACTC AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42493
    Clone ID Related Accession (Same CDS sequence) XM_009298502.2 , XM_009298502.1
    Accession Version XM_009298502.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296777.1
    CDS452..3589
    Translation

    Target ORF information:

    RefSeq Version XM_009298502.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298502.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42493
    Clone ID Related Accession (Same CDS sequence) XM_009298502.2 , XM_009298502.1
    Accession Version XM_009298502.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296777.1
    CDS211..3348
    Translation

    Target ORF information:

    RefSeq Version XM_009298502.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
    ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA
    TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA
    TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT
    CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA
    TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA
    ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC
    ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA
    AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT
    GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT
    CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA
    AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT
    TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA
    AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT
    GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA
    CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT
    GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA
    GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG
    GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA
    GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC
    TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT
    ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG
    TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC
    CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC
    AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA
    GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC
    CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA
    TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC
    GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG
    GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT
    GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA
    CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC
    ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG
    CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG
    ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC
    CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC
    AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA
    GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC
    ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG
    TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT
    AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA
    GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA
    CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT
    CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA
    TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA
    GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG
    CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG
    ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA
    TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG
    GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC
    AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG
    TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA
    AGCTCTGAAT ATGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.