ube3b cDNA ORF clone, Danio rerio(zebrafish)

The following ube3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa10273 NM_001114682.1
Latest version!
Danio rerio ubiquitin protein ligase E3B (ube3b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa10273 XM_005165149.4
Latest version!
Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa10273 XM_005165148.4
Latest version!
Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa10273 XM_005165149.3 Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa10273 XM_005165148.3 Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa10273
    Clone ID Related Accession (Same CDS sequence) XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
    Accession Version NM_001114682.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product ubiquitin-protein ligase E3B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX510366.5. On Feb 21, 2008 this sequence version replaced XM_679786.2. ##Evidence-Data-START## CDS exon combination :: GDQH01006749.1, GFIL01042576.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA4476738 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001108154.1
    CDS8..3217
    Translation

    Target ORF information:

    RefSeq Version NM_001114682.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3B (ube3b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001114682.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10273
    Clone ID Related Accession (Same CDS sequence) XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
    Accession Version XM_005165149.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165149.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165206.1
    CDS275..3484
    Translation

    Target ORF information:

    RefSeq Version XM_005165149.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165149.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10273
    Clone ID Related Accession (Same CDS sequence) XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
    Accession Version XM_005165149.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165206.1
    CDS294..3503
    Translation

    Target ORF information:

    RefSeq Version XM_005165149.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165149.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10273
    Clone ID Related Accession (Same CDS sequence) XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
    Accession Version XM_005165148.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165205.1
    CDS208..3417
    Translation

    Target ORF information:

    RefSeq Version XM_005165148.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165148.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10273
    Clone ID Related Accession (Same CDS sequence) XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
    Accession Version XM_005165148.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165148.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165205.1
    CDS189..3398
    Translation

    Target ORF information:

    RefSeq Version XM_005165148.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165148.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
    GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC
    AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC
    ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT
    AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA
    ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT
    CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA
    TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG
    TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG
    GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT
    CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA
    CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC
    GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA
    GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT
    GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT
    GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC
    TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG
    TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA
    GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA
    ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT
    CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA
    TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT
    GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG
    GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG
    CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC
    TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG
    GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC
    ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG
    CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT
    TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG
    GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG
    GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG
    CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC
    ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT
    GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG
    CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA
    GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC
    AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA
    TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG
    AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT
    TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG
    CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT
    GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT
    CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT
    ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC
    ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG
    GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC
    ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC
    CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC
    TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG
    GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC
    ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT
    CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC
    AGCATGAACA CTGGCTTTGA GCTGTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.