rusc2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rusc2
Entrez Gene ID	556081
Full Name	RUN and SH3 domain containing 2
Synonyms	si:ch211-250o15.2
General protein information	Preferred Names RUN and SH3 domain containing 2 Names iporin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165192.3	XP_005165249.1	iporin isoform X3
XM_005165191.3	XP_005165248.1	iporin isoform X2
XM_005165193.2	XP_005165250.1	iporin isoform X4
XM_005165193.3	XP_005165250.1	iporin isoform X4
XM_005165190.3	XP_005165247.1	iporin isoform X1
XM_005165190.4	XP_005165247.1	iporin isoform X1
XM_005165192.2	XP_005165249.1	iporin isoform X3
XM_005165191.4	XP_005165248.1	iporin isoform X2

Pathways from BioSystems

Homologies

	RUSC2	XP_002800131.1
Mus musculus (house mouse)	Rusc2	NP_001032798.1
Gallus gallus (chicken)	RUSC2	XP_003643016.1
Danio rerio (zebrafish)	rusc2	XP_005165247.1
Homo sapiens (human)	RUSC2	NP_001129471.1
Pan troglodytes (chimpanzee)	RUSC2	XP_520556.3
Canis lupus familiaris (dog)	RUSC2	XP_538715.3
Rattus norvegicus (Norway rat)	Rusc2	XP_003749975.1
Bos taurus (cattle)	RUSC2	NP_001075896.1
Xenopus tropicalis (tropical clawed frog)	rusc2	XP_002940237.2

Gene Ontology

The following rusc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rusc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10311	XM_005165192.3 Latest version!	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10312	XM_005165193.3 Latest version!	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa10309	XM_005165190.4 Latest version!	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa10310	XM_005165191.4 Latest version!	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa10312	XM_005165193.2	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa10309	XM_005165190.3	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa10311	XM_005165192.2	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10310	XM_005165191.3	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rusc2 ( XM_005165192.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165249.1 Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10311
Clone ID Related Accession (Same CDS sequence)	XM_005165192.3 , XM_005165192.2
Accession Version	XM_005165192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	iporin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC GAGGGGCTGC AGCACCAGAG GGTGATGAAG ATGACTCTGA AGAACACCAC 
AGGCCAGCTC ACGGCACCTC CAACACACCT ACAACAGTGC GGCCATCTCC ACTGGGAAGC 
TATTCCCCAA TTCGGCTCCA GGGTGCACCT ACCTCTTCTG GAAATTGCTC AACATGCTCT 
CCGACTCTGA ATGGTCCACG GCCACCACGT TCCCTCTCTT GCCCCATCTC TGCAGGTCTT 
CTGCCTCAGC ACACCCCACC TGGAGTGGCC ATACATACAG AGACTCCTAA ACTTGCTCCA 
TTGCCTCCAA CACCACCTCC AGCTCCCCTC ATGAAGCAGA GTCCATTGAT GCCTGCGTTG 
CCCACTAGAA ATCACTGCTT CCACCGGGGA AATTTGCCAG CGCTTCCCAG CTCTGGTCTA 
AGTCCTCTTG GACAGTTGGA GCCTGCACCG CAGGAAGAAC CAGTAAAGGT TTTACCGGCA 
TGCGTGACAC AGCGACAGCC TAATGTGCAC CACCTCTCCC CACAGGCACT CAAATGGAGA 
GAGTACAGGC GCAAGAACCC TCTGGGTGTG GAGCGCTGTA AAGATGGCCC TCATTCTTTC 
TCTTGTTCTT TGGAAACCAA GAGACCTGGG ACTCGTGTCA TGCGGCGCAA TGTCTTTGAT 
TTCCCACCAA CCAATCAAGC ACTCAGCTTC GGCAGACTGA ATGGTCAGTC ATTGAGGCAG 
CTGCCGCAGT GCTACAGCGA CTTCCTGCCT GATTATTTCT CTCAGACAGA GCGCCCTCCT 
GAGGAGTTCT GCTTATCTCC TGACGCTCAT ACTGATGAAT CCATCTCTAT AGACCTGTTG 
CAGAAAAGAG GTTTGGTGAA AGCCATCAAC ACTGCAGTCG ATCTGATAGT TGCTCATTTT 
GGCACCAGTC GGGATGCAGG TGTTAAGGCC AAATTGGGTA ATAGCTCAGT TAGTCCGAAT 
GTGGGTCACC TTATTCTGAA GTACTTGTGT CCGGCCATTC GGGAGATACT GAATGATGGA 
CTGAAAGCAT ACGTACTGGA CCTGATCATT GGACAACGCA AAAACCAGCC CTGGAGTGTG 
GTTGAAGCCT CCACACAACT TGGACCTTCT ACACGAGTTT TGCACAGCCT GTTTTCTAAA 
GTGAGCCAGT ATTCTGAGCT GACCAATCAC AGTATGAGAC TCAATGCCTT CATCTTTGGC 
CTGCTAAACT TGCGATCTCT GGAATTCTGG TTCAATCACA TTTATACGCA TGAAGACATC 
ATAGCAGCAC ATTACCACCC ATGGGGTTTC CTACCGCTGT CTCAAGGAGC TTGCCAGCCT 
CTTTTTGAAG AGTTGATGCT CTTGCTTCAG CCACTGTCAC TGCTTCCCTT TGACCTTGAC 
CTGCTATTTG AGCCACACCA GCTTCAGAAA GGAGAAGAAC ACCTGAGACG CAAAGAACAG 
CTGTGCTCTG CTCGCCAGAG CCTCGACCAA TCAGATCGCT CCACTTTCCA ACTCATGAGA 
GGCTGTGGAA TGTTGGAGTC TCAGGAAACG GGAATGCTGC CTCAGAGAGA AAGCTTCGTG 
CTGAGAGATG AGGAGTGCCG GCTTAGGACT GAGGGAGTGA CATTAAAAAT GAAACAAGAA 
TGGCAGAGGA GTGTGGGAGC AGAACGTGAG TTTCGGAATA AGGAGGATGG AGTTGGTAAA 
GAGTGCTTGC GAAAGAGTGA CACAGGACAG ATGCAAGGTG GAGGGTGCTG TGCTGCCAGA 
ATGAAGGGAG TTGGAGAAGA CAATGAGAAG GACAAAAAGA GAGAAAAAGA TGGAGATGCA 
ACCAAACAGA GAAACCGACA AGCTGGCTGG TGGTATCAGT TGATGCAAAG CTCACAGGTT 
TACATTGACA ACTCTGCCGA GGGGTCGAAA TTTGTCAAGT GGGAGAAGAG GAGGAAAGGA 
GGGGTTGAGA ATCACCGTCA AAGCCATCCA CCTCCTAGAG AAGGTGTTGT TGAAGGAGCT 
GAGGCCATTC AGCAGATGGA TGAACAGGTG GAACACGGTC GGACCAGTAG TAGCAATATT 
AATAGCATTG GCCGTGGGGT TCTAAACCAA TCAGCTTTGT CAGAACCCAC CAAAGCCACA 
AAAGCGAAGC CATCTTGGAT GGGAAGTCCA CCGGAGTCAG TGCTCACTGA GCTGAAAAAG 
AGTAAGGAGA AGCAGCCAGA TTGCCAGGAT CCGAATGAAG GGCTTCAGGC CCAGCCAGAA 
GCAGAGGAGG ATGGAACGGC TCAAGTGCTC CGCTGGGGAC GACTATTTGG AGCTGGAAAT 
GCAAGCAAGA AAGAAAAGAT TGAACAGAAA TCAGTCAGGA AACAAAGATT GCCTTCAGGT 
TGGTTGAGTA TCGATCATTC TGTGTTGGAT CTGGTGGCTC AGTCTGTTGG AGTTCGGAAA 
AGAGCGGAGC AACAATCTTT TACATCTCAA AGCCAAGAAT CCAACCTTGG CCCAACTGAA 
CAAGCACAAA CACAGAGTCC CCAAACAAAG ACACTAGCCC CACGGGAAGT GAAGGCGTTA 
TGCCATCATA TTGCCACAGA GCCAGGGCAG CTCAGTTTCA ACAAAGGTGA CGTGCTGCAG 
GTGCTCAGCA GGGCAGACTC TGATTGGCTG ATGTGTGCTC TGGGCGATAG CCAGGGCCTT 
GTTCCCATCA TATACGTCAC CCTGATTGAA GACAGTCAGG AGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165249.1
CDS	303..2990
Translation	MQSRGAAAPEGDEDDSEEHHRPAHGTSNTPTTVRPSPLGSYSPIRLQGAPTSSGNCSTCSPTLNGPRPPRSLSCPISAGLLPQHTPPGVAIHTETPKLAPLPPTPPPAPLMKQSPLMPALPTRNHCFHRGNLPALPSSGLSPLGQLEPAPQEEPVKVLPACVTQRQPNVHHLSPQALKWREYRRKNPLGVERCKDGPHSFSCSLETKRPGTRVMRRNVFDFPPTNQALSFGRLNGQSLRQLPQCYSDFLPDYFSQTERPPEEFCLSPDAHTDESISIDLLQKRGLVKAINTAVDLIVAHFGTSRDAGVKAKLGNSSVSPNVGHLILKYLCPAIREILNDGLKAYVLDLIIGQRKNQPWSVVEASTQLGPSTRVLHSLFSKVSQYSELTNHSMRLNAFIFGLLNLRSLEFWFNHIYTHEDIIAAHYHPWGFLPLSQGACQPLFEELMLLLQPLSLLPFDLDLLFEPHQLQKGEEHLRRKEQLCSARQSLDQSDRSTFQLMRGCGMLESQETGMLPQRESFVLRDEECRLRTEGVTLKMKQEWQRSVGAEREFRNKEDGVGKECLRKSDTGQMQGGGCCAARMKGVGEDNEKDKKREKDGDATKQRNRQAGWWYQLMQSSQVYIDNSAEGSKFVKWEKRRKGGVENHRQSHPPPREGVVEGAEAIQQMDEQVEHGRTSSSNINSIGRGVLNQSALSEPTKATKAKPSWMGSPPESVLTELKKSKEKQPDCQDPNEGLQAQPEAEEDGTAQVLRWGRLFGAGNASKKEKIEQKSVRKQRLPSGWLSIDHSVLDLVAQSVGVRKRAEQQSFTSQSQESNLGPTEQAQTQSPQTKTLAPREVKALCHHIATEPGQLSFNKGDVLQVLSRADSDWLMCALGDSQGLVPIIYVTLIEDSQER

Target ORF information:

RefSeq Version	XM_005165192.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165192.3

ORF Insert Sequence:

ATGCAGAGTC GAGGGGCTGC AGCACCAGAG GGTGATGAAG ATGACTCTGA AGAACACCAC 
AGGCCAGCTC ACGGCACCTC CAACACACCT ACAACAGTGC GGCCATCTCC ACTGGGAAGC 
TATTCCCCAA TTCGGCTCCA GGGTGCACCT ACCTCTTCTG GAAATTGCTC AACATGCTCT 
CCGACTCTGA ATGGTCCACG GCCACCACGT TCCCTCTCTT GCCCCATCTC TGCAGGTCTT 
CTGCCTCAGC ACACCCCACC TGGAGTGGCC ATACATACAG AGACTCCTAA ACTTGCTCCA 
TTGCCTCCAA CACCACCTCC AGCTCCCCTC ATGAAGCAGA GTCCATTGAT GCCTGCGTTG 
CCCACTAGAA ATCACTGCTT CCACCGGGGA AATTTGCCAG CGCTTCCCAG CTCTGGTCTA 
AGTCCTCTTG GACAGTTGGA GCCTGCACCG CAGGAAGAAC CAGTAAAGGT TTTACCGGCA 
TGCGTGACAC AGCGACAGCC TAATGTGCAC CACCTCTCCC CACAGGCACT CAAATGGAGA 
GAGTACAGGC GCAAGAACCC TCTGGGTGTG GAGCGCTGTA AAGATGGCCC TCATTCTTTC 
TCTTGTTCTT TGGAAACCAA GAGACCTGGG ACTCGTGTCA TGCGGCGCAA TGTCTTTGAT 
TTCCCACCAA CCAATCAAGC ACTCAGCTTC GGCAGACTGA ATGGTCAGTC ATTGAGGCAG 
CTGCCGCAGT GCTACAGCGA CTTCCTGCCT GATTATTTCT CTCAGACAGA GCGCCCTCCT 
GAGGAGTTCT GCTTATCTCC TGACGCTCAT ACTGATGAAT CCATCTCTAT AGACCTGTTG 
CAGAAAAGAG GTTTGGTGAA AGCCATCAAC ACTGCAGTCG ATCTGATAGT TGCTCATTTT 
GGCACCAGTC GGGATGCAGG TGTTAAGGCC AAATTGGGTA ATAGCTCAGT TAGTCCGAAT 
GTGGGTCACC TTATTCTGAA GTACTTGTGT CCGGCCATTC GGGAGATACT GAATGATGGA 
CTGAAAGCAT ACGTACTGGA CCTGATCATT GGACAACGCA AAAACCAGCC CTGGAGTGTG 
GTTGAAGCCT CCACACAACT TGGACCTTCT ACACGAGTTT TGCACAGCCT GTTTTCTAAA 
GTGAGCCAGT ATTCTGAGCT GACCAATCAC AGTATGAGAC TCAATGCCTT CATCTTTGGC 
CTGCTAAACT TGCGATCTCT GGAATTCTGG TTCAATCACA TTTATACGCA TGAAGACATC 
ATAGCAGCAC ATTACCACCC ATGGGGTTTC CTACCGCTGT CTCAAGGAGC TTGCCAGCCT 
CTTTTTGAAG AGTTGATGCT CTTGCTTCAG CCACTGTCAC TGCTTCCCTT TGACCTTGAC 
CTGCTATTTG AGCCACACCA GCTTCAGAAA GGAGAAGAAC ACCTGAGACG CAAAGAACAG 
CTGTGCTCTG CTCGCCAGAG CCTCGACCAA TCAGATCGCT CCACTTTCCA ACTCATGAGA 
GGCTGTGGAA TGTTGGAGTC TCAGGAAACG GGAATGCTGC CTCAGAGAGA AAGCTTCGTG 
CTGAGAGATG AGGAGTGCCG GCTTAGGACT GAGGGAGTGA CATTAAAAAT GAAACAAGAA 
TGGCAGAGGA GTGTGGGAGC AGAACGTGAG TTTCGGAATA AGGAGGATGG AGTTGGTAAA 
GAGTGCTTGC GAAAGAGTGA CACAGGACAG ATGCAAGGTG GAGGGTGCTG TGCTGCCAGA 
ATGAAGGGAG TTGGAGAAGA CAATGAGAAG GACAAAAAGA GAGAAAAAGA TGGAGATGCA 
ACCAAACAGA GAAACCGACA AGCTGGCTGG TGGTATCAGT TGATGCAAAG CTCACAGGTT 
TACATTGACA ACTCTGCCGA GGGGTCGAAA TTTGTCAAGT GGGAGAAGAG GAGGAAAGGA 
GGGGTTGAGA ATCACCGTCA AAGCCATCCA CCTCCTAGAG AAGGTGTTGT TGAAGGAGCT 
GAGGCCATTC AGCAGATGGA TGAACAGGTG GAACACGGTC GGACCAGTAG TAGCAATATT 
AATAGCATTG GCCGTGGGGT TCTAAACCAA TCAGCTTTGT CAGAACCCAC CAAAGCCACA 
AAAGCGAAGC CATCTTGGAT GGGAAGTCCA CCGGAGTCAG TGCTCACTGA GCTGAAAAAG 
AGTAAGGAGA AGCAGCCAGA TTGCCAGGAT CCGAATGAAG GGCTTCAGGC CCAGCCAGAA 
GCAGAGGAGG ATGGAACGGC TCAAGTGCTC CGCTGGGGAC GACTATTTGG AGCTGGAAAT 
GCAAGCAAGA AAGAAAAGAT TGAACAGAAA TCAGTCAGGA AACAAAGATT GCCTTCAGGT 
TGGTTGAGTA TCGATCATTC TGTGTTGGAT CTGGTGGCTC AGTCTGTTGG AGTTCGGAAA 
AGAGCGGAGC AACAATCTTT TACATCTCAA AGCCAAGAAT CCAACCTTGG CCCAACTGAA 
CAAGCACAAA CACAGAGTCC CCAAACAAAG ACACTAGCCC CACGGGAAGT GAAGGCGTTA 
TGCCATCATA TTGCCACAGA GCCAGGGCAG CTCAGTTTCA ACAAAGGTGA CGTGCTGCAG 
GTGCTCAGCA GGGCAGACTC TGATTGGCTG ATGTGTGCTC TGGGCGATAG CCAGGGCCTT 
GTTCCCATCA TATACGTCAC CCTGATTGAA GACAGTCAGG AGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_005165192.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165249.1 Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10311
Clone ID Related Accession (Same CDS sequence)	XM_005165192.3 , XM_005165192.2
Accession Version	XM_005165192.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	iporin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC GAGGGGCTGC AGCACCAGAG GGTGATGAAG ATGACTCTGA AGAACACCAC 
AGGCCAGCTC ACGGCACCTC CAACACACCT ACAACAGTGC GGCCATCTCC ACTGGGAAGC 
TATTCCCCAA TTCGGCTCCA GGGTGCACCT ACCTCTTCTG GAAATTGCTC AACATGCTCT 
CCGACTCTGA ATGGTCCACG GCCACCACGT TCCCTCTCTT GCCCCATCTC TGCAGGTCTT 
CTGCCTCAGC ACACCCCACC TGGAGTGGCC ATACATACAG AGACTCCTAA ACTTGCTCCA 
TTGCCTCCAA CACCACCTCC AGCTCCCCTC ATGAAGCAGA GTCCATTGAT GCCTGCGTTG 
CCCACTAGAA ATCACTGCTT CCACCGGGGA AATTTGCCAG CGCTTCCCAG CTCTGGTCTA 
AGTCCTCTTG GACAGTTGGA GCCTGCACCG CAGGAAGAAC CAGTAAAGGT TTTACCGGCA 
TGCGTGACAC AGCGACAGCC TAATGTGCAC CACCTCTCCC CACAGGCACT CAAATGGAGA 
GAGTACAGGC GCAAGAACCC TCTGGGTGTG GAGCGCTGTA AAGATGGCCC TCATTCTTTC 
TCTTGTTCTT TGGAAACCAA GAGACCTGGG ACTCGTGTCA TGCGGCGCAA TGTCTTTGAT 
TTCCCACCAA CCAATCAAGC ACTCAGCTTC GGCAGACTGA ATGGTCAGTC ATTGAGGCAG 
CTGCCGCAGT GCTACAGCGA CTTCCTGCCT GATTATTTCT CTCAGACAGA GCGCCCTCCT 
GAGGAGTTCT GCTTATCTCC TGACGCTCAT ACTGATGAAT CCATCTCTAT AGACCTGTTG 
CAGAAAAGAG GTTTGGTGAA AGCCATCAAC ACTGCAGTCG ATCTGATAGT TGCTCATTTT 
GGCACCAGTC GGGATGCAGG TGTTAAGGCC AAATTGGGTA ATAGCTCAGT TAGTCCGAAT 
GTGGGTCACC TTATTCTGAA GTACTTGTGT CCGGCCATTC GGGAGATACT GAATGATGGA 
CTGAAAGCAT ACGTACTGGA CCTGATCATT GGACAACGCA AAAACCAGCC CTGGAGTGTG 
GTTGAAGCCT CCACACAACT TGGACCTTCT ACACGAGTTT TGCACAGCCT GTTTTCTAAA 
GTGAGCCAGT ATTCTGAGCT GACCAATCAC AGTATGAGAC TCAATGCCTT CATCTTTGGC 
CTGCTAAACT TGCGATCTCT GGAATTCTGG TTCAATCACA TTTATACGCA TGAAGACATC 
ATAGCAGCAC ATTACCACCC ATGGGGTTTC CTACCGCTGT CTCAAGGAGC TTGCCAGCCT 
CTTTTTGAAG AGTTGATGCT CTTGCTTCAG CCACTGTCAC TGCTTCCCTT TGACCTTGAC 
CTGCTATTTG AGCCACACCA GCTTCAGAAA GGAGAAGAAC ACCTGAGACG CAAAGAACAG 
CTGTGCTCTG CTCGCCAGAG CCTCGACCAA TCAGATCGCT CCACTTTCCA ACTCATGAGA 
GGCTGTGGAA TGTTGGAGTC TCAGGAAACG GGAATGCTGC CTCAGAGAGA AAGCTTCGTG 
CTGAGAGATG AGGAGTGCCG GCTTAGGACT GAGGGAGTGA CATTAAAAAT GAAACAAGAA 
TGGCAGAGGA GTGTGGGAGC AGAACGTGAG TTTCGGAATA AGGAGGATGG AGTTGGTAAA 
GAGTGCTTGC GAAAGAGTGA CACAGGACAG ATGCAAGGTG GAGGGTGCTG TGCTGCCAGA 
ATGAAGGGAG TTGGAGAAGA CAATGAGAAG GACAAAAAGA GAGAAAAAGA TGGAGATGCA 
ACCAAACAGA GAAACCGACA AGCTGGCTGG TGGTATCAGT TGATGCAAAG CTCACAGGTT 
TACATTGACA ACTCTGCCGA GGGGTCGAAA TTTGTCAAGT GGGAGAAGAG GAGGAAAGGA 
GGGGTTGAGA ATCACCGTCA AAGCCATCCA CCTCCTAGAG AAGGTGTTGT TGAAGGAGCT 
GAGGCCATTC AGCAGATGGA TGAACAGGTG GAACACGGTC GGACCAGTAG TAGCAATATT 
AATAGCATTG GCCGTGGGGT TCTAAACCAA TCAGCTTTGT CAGAACCCAC CAAAGCCACA 
AAAGCGAAGC CATCTTGGAT GGGAAGTCCA CCGGAGTCAG TGCTCACTGA GCTGAAAAAG 
AGTAAGGAGA AGCAGCCAGA TTGCCAGGAT CCGAATGAAG GGCTTCAGGC CCAGCCAGAA 
GCAGAGGAGG ATGGAACGGC TCAAGTGCTC CGCTGGGGAC GACTATTTGG AGCTGGAAAT 
GCAAGCAAGA AAGAAAAGAT TGAACAGAAA TCAGTCAGGA AACAAAGATT GCCTTCAGGT 
TGGTTGAGTA TCGATCATTC TGTGTTGGAT CTGGTGGCTC AGTCTGTTGG AGTTCGGAAA 
AGAGCGGAGC AACAATCTTT TACATCTCAA AGCCAAGAAT CCAACCTTGG CCCAACTGAA 
CAAGCACAAA CACAGAGTCC CCAAACAAAG ACACTAGCCC CACGGGAAGT GAAGGCGTTA 
TGCCATCATA TTGCCACAGA GCCAGGGCAG CTCAGTTTCA ACAAAGGTGA CGTGCTGCAG 
GTGCTCAGCA GGGCAGACTC TGATTGGCTG ATGTGTGCTC TGGGCGATAG CCAGGGCCTT 
GTTCCCATCA TATACGTCAC CCTGATTGAA GACAGTCAGG AGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165249.1
CDS	308..2995
Translation	MQSRGAAAPEGDEDDSEEHHRPAHGTSNTPTTVRPSPLGSYSPIRLQGAPTSSGNCSTCSPTLNGPRPPRSLSCPISAGLLPQHTPPGVAIHTETPKLAPLPPTPPPAPLMKQSPLMPALPTRNHCFHRGNLPALPSSGLSPLGQLEPAPQEEPVKVLPACVTQRQPNVHHLSPQALKWREYRRKNPLGVERCKDGPHSFSCSLETKRPGTRVMRRNVFDFPPTNQALSFGRLNGQSLRQLPQCYSDFLPDYFSQTERPPEEFCLSPDAHTDESISIDLLQKRGLVKAINTAVDLIVAHFGTSRDAGVKAKLGNSSVSPNVGHLILKYLCPAIREILNDGLKAYVLDLIIGQRKNQPWSVVEASTQLGPSTRVLHSLFSKVSQYSELTNHSMRLNAFIFGLLNLRSLEFWFNHIYTHEDIIAAHYHPWGFLPLSQGACQPLFEELMLLLQPLSLLPFDLDLLFEPHQLQKGEEHLRRKEQLCSARQSLDQSDRSTFQLMRGCGMLESQETGMLPQRESFVLRDEECRLRTEGVTLKMKQEWQRSVGAEREFRNKEDGVGKECLRKSDTGQMQGGGCCAARMKGVGEDNEKDKKREKDGDATKQRNRQAGWWYQLMQSSQVYIDNSAEGSKFVKWEKRRKGGVENHRQSHPPPREGVVEGAEAIQQMDEQVEHGRTSSSNINSIGRGVLNQSALSEPTKATKAKPSWMGSPPESVLTELKKSKEKQPDCQDPNEGLQAQPEAEEDGTAQVLRWGRLFGAGNASKKEKIEQKSVRKQRLPSGWLSIDHSVLDLVAQSVGVRKRAEQQSFTSQSQESNLGPTEQAQTQSPQTKTLAPREVKALCHHIATEPGQLSFNKGDVLQVLSRADSDWLMCALGDSQGLVPIIYVTLIEDSQER

Target ORF information:

RefSeq Version	XM_005165192.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165192.2

ORF Insert Sequence:

ATGCAGAGTC GAGGGGCTGC AGCACCAGAG GGTGATGAAG ATGACTCTGA AGAACACCAC 
AGGCCAGCTC ACGGCACCTC CAACACACCT ACAACAGTGC GGCCATCTCC ACTGGGAAGC 
TATTCCCCAA TTCGGCTCCA GGGTGCACCT ACCTCTTCTG GAAATTGCTC AACATGCTCT 
CCGACTCTGA ATGGTCCACG GCCACCACGT TCCCTCTCTT GCCCCATCTC TGCAGGTCTT 
CTGCCTCAGC ACACCCCACC TGGAGTGGCC ATACATACAG AGACTCCTAA ACTTGCTCCA 
TTGCCTCCAA CACCACCTCC AGCTCCCCTC ATGAAGCAGA GTCCATTGAT GCCTGCGTTG 
CCCACTAGAA ATCACTGCTT CCACCGGGGA AATTTGCCAG CGCTTCCCAG CTCTGGTCTA 
AGTCCTCTTG GACAGTTGGA GCCTGCACCG CAGGAAGAAC CAGTAAAGGT TTTACCGGCA 
TGCGTGACAC AGCGACAGCC TAATGTGCAC CACCTCTCCC CACAGGCACT CAAATGGAGA 
GAGTACAGGC GCAAGAACCC TCTGGGTGTG GAGCGCTGTA AAGATGGCCC TCATTCTTTC 
TCTTGTTCTT TGGAAACCAA GAGACCTGGG ACTCGTGTCA TGCGGCGCAA TGTCTTTGAT 
TTCCCACCAA CCAATCAAGC ACTCAGCTTC GGCAGACTGA ATGGTCAGTC ATTGAGGCAG 
CTGCCGCAGT GCTACAGCGA CTTCCTGCCT GATTATTTCT CTCAGACAGA GCGCCCTCCT 
GAGGAGTTCT GCTTATCTCC TGACGCTCAT ACTGATGAAT CCATCTCTAT AGACCTGTTG 
CAGAAAAGAG GTTTGGTGAA AGCCATCAAC ACTGCAGTCG ATCTGATAGT TGCTCATTTT 
GGCACCAGTC GGGATGCAGG TGTTAAGGCC AAATTGGGTA ATAGCTCAGT TAGTCCGAAT 
GTGGGTCACC TTATTCTGAA GTACTTGTGT CCGGCCATTC GGGAGATACT GAATGATGGA 
CTGAAAGCAT ACGTACTGGA CCTGATCATT GGACAACGCA AAAACCAGCC CTGGAGTGTG 
GTTGAAGCCT CCACACAACT TGGACCTTCT ACACGAGTTT TGCACAGCCT GTTTTCTAAA 
GTGAGCCAGT ATTCTGAGCT GACCAATCAC AGTATGAGAC TCAATGCCTT CATCTTTGGC 
CTGCTAAACT TGCGATCTCT GGAATTCTGG TTCAATCACA TTTATACGCA TGAAGACATC 
ATAGCAGCAC ATTACCACCC ATGGGGTTTC CTACCGCTGT CTCAAGGAGC TTGCCAGCCT 
CTTTTTGAAG AGTTGATGCT CTTGCTTCAG CCACTGTCAC TGCTTCCCTT TGACCTTGAC 
CTGCTATTTG AGCCACACCA GCTTCAGAAA GGAGAAGAAC ACCTGAGACG CAAAGAACAG 
CTGTGCTCTG CTCGCCAGAG CCTCGACCAA TCAGATCGCT CCACTTTCCA ACTCATGAGA 
GGCTGTGGAA TGTTGGAGTC TCAGGAAACG GGAATGCTGC CTCAGAGAGA AAGCTTCGTG 
CTGAGAGATG AGGAGTGCCG GCTTAGGACT GAGGGAGTGA CATTAAAAAT GAAACAAGAA 
TGGCAGAGGA GTGTGGGAGC AGAACGTGAG TTTCGGAATA AGGAGGATGG AGTTGGTAAA 
GAGTGCTTGC GAAAGAGTGA CACAGGACAG ATGCAAGGTG GAGGGTGCTG TGCTGCCAGA 
ATGAAGGGAG TTGGAGAAGA CAATGAGAAG GACAAAAAGA GAGAAAAAGA TGGAGATGCA 
ACCAAACAGA GAAACCGACA AGCTGGCTGG TGGTATCAGT TGATGCAAAG CTCACAGGTT 
TACATTGACA ACTCTGCCGA GGGGTCGAAA TTTGTCAAGT GGGAGAAGAG GAGGAAAGGA 
GGGGTTGAGA ATCACCGTCA AAGCCATCCA CCTCCTAGAG AAGGTGTTGT TGAAGGAGCT 
GAGGCCATTC AGCAGATGGA TGAACAGGTG GAACACGGTC GGACCAGTAG TAGCAATATT 
AATAGCATTG GCCGTGGGGT TCTAAACCAA TCAGCTTTGT CAGAACCCAC CAAAGCCACA 
AAAGCGAAGC CATCTTGGAT GGGAAGTCCA CCGGAGTCAG TGCTCACTGA GCTGAAAAAG 
AGTAAGGAGA AGCAGCCAGA TTGCCAGGAT CCGAATGAAG GGCTTCAGGC CCAGCCAGAA 
GCAGAGGAGG ATGGAACGGC TCAAGTGCTC CGCTGGGGAC GACTATTTGG AGCTGGAAAT 
GCAAGCAAGA AAGAAAAGAT TGAACAGAAA TCAGTCAGGA AACAAAGATT GCCTTCAGGT 
TGGTTGAGTA TCGATCATTC TGTGTTGGAT CTGGTGGCTC AGTCTGTTGG AGTTCGGAAA 
AGAGCGGAGC AACAATCTTT TACATCTCAA AGCCAAGAAT CCAACCTTGG CCCAACTGAA 
CAAGCACAAA CACAGAGTCC CCAAACAAAG ACACTAGCCC CACGGGAAGT GAAGGCGTTA 
TGCCATCATA TTGCCACAGA GCCAGGGCAG CTCAGTTTCA ACAAAGGTGA CGTGCTGCAG 
GTGCTCAGCA GGGCAGACTC TGATTGGCTG ATGTGTGCTC TGGGCGATAG CCAGGGCCTT 
GTTCCCATCA TATACGTCAC CCTGATTGAA GACAGTCAGG AGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_005165193.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165250.1 Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa10312
Clone ID Related Accession (Same CDS sequence)	XM_005165193.2 , XM_005165193.3
Accession Version	XM_005165193.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	iporin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC GAGGGGCTGC AGCACCAGAG GGTGATGAAG ATGACTCTGA AGAACACCAC 
AGGCCAGCTC ACGGCACCTC CAACACACCT ACAACAGTGC GGCCATCTCC ACTGGGAAGC 
TATTCCCCAA TTCGGCTCCA GGGTGCACCT ACCTCTTCTG GAAATTGCTC AACATGCTCT 
CCGACTCTGA ATGGTCCACG GCCACCACGT TCCCTCTCTT GCCCCATCTC TGCAGGTCTT 
CTGCCTCAGC ACACCCCACC TGGAGTGGCC ATACATACAG AGACTCCTAA ACTTGCTCCA 
TTGCCTCCAA CACCACCTCC AGCTCCCCTC ATGAAGCAGA GTCCATTGAT GCCTGCGTTG 
CCCACTAGAA ATCACTGCTT CCACCGGGGA AATTTGCCAG CGCTTCCCAG CTCTGGTCTA 
AGTCCTCTTG GACAGTTGGA GCCTGCACCG CAGGAAGAAC CAGTAAAGGT TTTACCGGCA 
TGCGTGACAC AGCGACAGCC TAATGGTCAG TCATTGAGGC AGCTGCCGCA GTGCTACAGC 
GACTTCCTGC CTGATTATTT CTCTCAGACA GAGCGCCCTC CTGAGGAGTT CTGCTTATCT 
CCTGACGCTC ATACTGATGA ATCCATCTCT ATAGACCTGT TGCAGAAAAG AGGTTTGGTG 
AAAGCCATCA ACACTGCAGT CGATCTGATA GTTGCTCATT TTGGCACCAG TCGGGATGCA 
GGTGTTAAGG CCAAATTGGG TAATAGCTCA GTTAGTCCGA ATGTGGGTCA CCTTATTCTG 
AAGTACTTGT GTCCGGCCAT TCGGGAGATA CTGAATGATG GACTGAAAGC ATACGTACTG 
GACCTGATCA TTGGACAACG CAAAAACCAG CCCTGGAGTG TGGTTGAAGC CTCCACACAA 
CTTGGACCTT CTACACGAGT TTTGCACAGC CTGTTTTCTA AAGTGAGCCA GTATTCTGAG 
CTGACCAATC ACAGTATGAG ACTCAATGCC TTCATCTTTG GCCTGCTAAA CTTGCGATCT 
CTGGAATTCT GGTTCAATCA CATTTATACG CATGAAGACA TCATAGCAGC ACATTACCAC 
CCATGGGGTT TCCTACCGCT GTCTCAAGGA GCTTGCCAGC CTCTTTTTGA AGAGTTGATG 
CTCTTGCTTC AGCCACTGTC ACTGCTTCCC TTTGACCTTG ACCTGCTATT TGAGCCACAC 
CAGCTTCAGA AAGGAGAAGA ACACCTGAGA CGCAAAGAAC AGCTGTGCTC TGCTCGCCAG 
AGCCTCGACC AATCAGATCG CTCCACTTTC CAACTCATGA GAGGCTGTGG AATGTTGGAG 
TCTCAGGAAA CGGGAATGCT GCCTCAGAGA GAAAGCTTCG TGCTGAGAGA TGAGGAGTGC 
CGGCTTAGGA CTGAGGGAGT GACATTAAAA ATGAAACAAG AATGGCAGAG GAGTGTGGGA 
GCAGAACGTG AGTTTCGGAA TAAGGAGGAT GGAGTTGGTA AAGAGTGCTT GCGAAAGAGT 
GACACAGGAC AGATGCAAGG TGGAGGGTGC TGTGCTGCCA GAATGAAGGG AGTTGGAGAA 
GACAATGAGA AGGACAAAAA GAGAGAAAAA GATGGAGATG CAACCAAACA GAGAAACCGA 
CAAGCTGGCT GGTGGTATCA GTTGATGCAA AGCTCACAGG TTTACATTGA CAACTCTGCC 
GAGGGGTCGA AATTTGTCAA GTGGGAGAAG AGGAGGAAAG GAGGGGTTGA GAATCACCGT 
CAAAGCCATC CACCTCCTAG AGAAGGTGTT GTTGAAGGAG CTGAGGCCAT TCAGCAGATG 
GATGAACAGG TGGAACACGG TCGGACCAGT AGTAGCAATA TTAATAGCAT TGGCCGTGGG 
GTTCTAAACC AATCAGCTTT GTCAGAACCC ACCAAAGCCA CAAAAGCGAA GCCATCTTGG 
ATGGGAAGTC CACCGGAGTC AGTGCTCACT GAGCTGAAAA AGAGTAAGGA GAAGCAGCCA 
GATTGCCAGG ATCCGAATGA AGGGCTTCAG GCCCAGCCAG AAGCAGAGGA GGATGGAACG 
GCTCAAGTGC TCCGCTGGGG ACGACTATTT GGAGCTGGAA ATGCAAGCAA GAAAGAAAAG 
ATTGAACAGA AATCAGTCAG GAAACAAAGA TTGCCTTCAG GTTGGTTGAG TATCGATCAT 
TCTGTGTTGG ATCTGGTGGC TCAGTCTGTT GGAGTTCGGA AAAGAGCGGA GCAACAATCT 
TTTACATCTC AAAGCCAAGA ATCCAACCTT GGCCCAACTG AACAAGCACA AACACAGAGT 
CCCCAAACAA AGACACTAGC CCCACGGGAA GTGAAGGCGT TATGCCATCA TATTGCCACA 
GAGCCAGGGC AGCTCAGTTT CAACAAAGGT GACGTGCTGC AGGTGCTCAG CAGGGCAGAC 
TCTGATTGGC TGATGTGTGC TCTGGGCGAT AGCCAGGGCC TTGTTCCCAT CATATACGTC 
ACCCTGATTG AAGACAGTCA GGAGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165250.1
CDS	308..2797
Translation	MQSRGAAAPEGDEDDSEEHHRPAHGTSNTPTTVRPSPLGSYSPIRLQGAPTSSGNCSTCSPTLNGPRPPRSLSCPISAGLLPQHTPPGVAIHTETPKLAPLPPTPPPAPLMKQSPLMPALPTRNHCFHRGNLPALPSSGLSPLGQLEPAPQEEPVKVLPACVTQRQPNGQSLRQLPQCYSDFLPDYFSQTERPPEEFCLSPDAHTDESISIDLLQKRGLVKAINTAVDLIVAHFGTSRDAGVKAKLGNSSVSPNVGHLILKYLCPAIREILNDGLKAYVLDLIIGQRKNQPWSVVEASTQLGPSTRVLHSLFSKVSQYSELTNHSMRLNAFIFGLLNLRSLEFWFNHIYTHEDIIAAHYHPWGFLPLSQGACQPLFEELMLLLQPLSLLPFDLDLLFEPHQLQKGEEHLRRKEQLCSARQSLDQSDRSTFQLMRGCGMLESQETGMLPQRESFVLRDEECRLRTEGVTLKMKQEWQRSVGAEREFRNKEDGVGKECLRKSDTGQMQGGGCCAARMKGVGEDNEKDKKREKDGDATKQRNRQAGWWYQLMQSSQVYIDNSAEGSKFVKWEKRRKGGVENHRQSHPPPREGVVEGAEAIQQMDEQVEHGRTSSSNINSIGRGVLNQSALSEPTKATKAKPSWMGSPPESVLTELKKSKEKQPDCQDPNEGLQAQPEAEEDGTAQVLRWGRLFGAGNASKKEKIEQKSVRKQRLPSGWLSIDHSVLDLVAQSVGVRKRAEQQSFTSQSQESNLGPTEQAQTQSPQTKTLAPREVKALCHHIATEPGQLSFNKGDVLQVLSRADSDWLMCALGDSQGLVPIIYVTLIEDSQER

Target ORF information:

RefSeq Version	XM_005165193.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165193.2

ORF Insert Sequence:

ATGCAGAGTC GAGGGGCTGC AGCACCAGAG GGTGATGAAG ATGACTCTGA AGAACACCAC 
AGGCCAGCTC ACGGCACCTC CAACACACCT ACAACAGTGC GGCCATCTCC ACTGGGAAGC 
TATTCCCCAA TTCGGCTCCA GGGTGCACCT ACCTCTTCTG GAAATTGCTC AACATGCTCT 
CCGACTCTGA ATGGTCCACG GCCACCACGT TCCCTCTCTT GCCCCATCTC TGCAGGTCTT 
CTGCCTCAGC ACACCCCACC TGGAGTGGCC ATACATACAG AGACTCCTAA ACTTGCTCCA 
TTGCCTCCAA CACCACCTCC AGCTCCCCTC ATGAAGCAGA GTCCATTGAT GCCTGCGTTG 
CCCACTAGAA ATCACTGCTT CCACCGGGGA AATTTGCCAG CGCTTCCCAG CTCTGGTCTA 
AGTCCTCTTG GACAGTTGGA GCCTGCACCG CAGGAAGAAC CAGTAAAGGT TTTACCGGCA 
TGCGTGACAC AGCGACAGCC TAATGGTCAG TCATTGAGGC AGCTGCCGCA GTGCTACAGC 
GACTTCCTGC CTGATTATTT CTCTCAGACA GAGCGCCCTC CTGAGGAGTT CTGCTTATCT 
CCTGACGCTC ATACTGATGA ATCCATCTCT ATAGACCTGT TGCAGAAAAG AGGTTTGGTG 
AAAGCCATCA ACACTGCAGT CGATCTGATA GTTGCTCATT TTGGCACCAG TCGGGATGCA 
GGTGTTAAGG CCAAATTGGG TAATAGCTCA GTTAGTCCGA ATGTGGGTCA CCTTATTCTG 
AAGTACTTGT GTCCGGCCAT TCGGGAGATA CTGAATGATG GACTGAAAGC ATACGTACTG 
GACCTGATCA TTGGACAACG CAAAAACCAG CCCTGGAGTG TGGTTGAAGC CTCCACACAA 
CTTGGACCTT CTACACGAGT TTTGCACAGC CTGTTTTCTA AAGTGAGCCA GTATTCTGAG 
CTGACCAATC ACAGTATGAG ACTCAATGCC TTCATCTTTG GCCTGCTAAA CTTGCGATCT 
CTGGAATTCT GGTTCAATCA CATTTATACG CATGAAGACA TCATAGCAGC ACATTACCAC 
CCATGGGGTT TCCTACCGCT GTCTCAAGGA GCTTGCCAGC CTCTTTTTGA AGAGTTGATG 
CTCTTGCTTC AGCCACTGTC ACTGCTTCCC TTTGACCTTG ACCTGCTATT TGAGCCACAC 
CAGCTTCAGA AAGGAGAAGA ACACCTGAGA CGCAAAGAAC AGCTGTGCTC TGCTCGCCAG 
AGCCTCGACC AATCAGATCG CTCCACTTTC CAACTCATGA GAGGCTGTGG AATGTTGGAG 
TCTCAGGAAA CGGGAATGCT GCCTCAGAGA GAAAGCTTCG TGCTGAGAGA TGAGGAGTGC 
CGGCTTAGGA CTGAGGGAGT GACATTAAAA ATGAAACAAG AATGGCAGAG GAGTGTGGGA 
GCAGAACGTG AGTTTCGGAA TAAGGAGGAT GGAGTTGGTA AAGAGTGCTT GCGAAAGAGT 
GACACAGGAC AGATGCAAGG TGGAGGGTGC TGTGCTGCCA GAATGAAGGG AGTTGGAGAA 
GACAATGAGA AGGACAAAAA GAGAGAAAAA GATGGAGATG CAACCAAACA GAGAAACCGA 
CAAGCTGGCT GGTGGTATCA GTTGATGCAA AGCTCACAGG TTTACATTGA CAACTCTGCC 
GAGGGGTCGA AATTTGTCAA GTGGGAGAAG AGGAGGAAAG GAGGGGTTGA GAATCACCGT 
CAAAGCCATC CACCTCCTAG AGAAGGTGTT GTTGAAGGAG CTGAGGCCAT TCAGCAGATG 
GATGAACAGG TGGAACACGG TCGGACCAGT AGTAGCAATA TTAATAGCAT TGGCCGTGGG 
GTTCTAAACC AATCAGCTTT GTCAGAACCC ACCAAAGCCA CAAAAGCGAA GCCATCTTGG 
ATGGGAAGTC CACCGGAGTC AGTGCTCACT GAGCTGAAAA AGAGTAAGGA GAAGCAGCCA 
GATTGCCAGG ATCCGAATGA AGGGCTTCAG GCCCAGCCAG AAGCAGAGGA GGATGGAACG 
GCTCAAGTGC TCCGCTGGGG ACGACTATTT GGAGCTGGAA ATGCAAGCAA GAAAGAAAAG 
ATTGAACAGA AATCAGTCAG GAAACAAAGA TTGCCTTCAG GTTGGTTGAG TATCGATCAT 
TCTGTGTTGG ATCTGGTGGC TCAGTCTGTT GGAGTTCGGA AAAGAGCGGA GCAACAATCT 
TTTACATCTC AAAGCCAAGA ATCCAACCTT GGCCCAACTG AACAAGCACA AACACAGAGT 
CCCCAAACAA AGACACTAGC CCCACGGGAA GTGAAGGCGT TATGCCATCA TATTGCCACA 
GAGCCAGGGC AGCTCAGTTT CAACAAAGGT GACGTGCTGC AGGTGCTCAG CAGGGCAGAC 
TCTGATTGGC TGATGTGTGC TCTGGGCGAT AGCCAGGGCC TTGTTCCCAT CATATACGTC 
ACCCTGATTG AAGACAGTCA GGAGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_005165193.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165250.1 Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa10312
Clone ID Related Accession (Same CDS sequence)	XM_005165193.2 , XM_005165193.3
Accession Version	XM_005165193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	iporin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC GAGGGGCTGC AGCACCAGAG GGTGATGAAG ATGACTCTGA AGAACACCAC 
AGGCCAGCTC ACGGCACCTC CAACACACCT ACAACAGTGC GGCCATCTCC ACTGGGAAGC 
TATTCCCCAA TTCGGCTCCA GGGTGCACCT ACCTCTTCTG GAAATTGCTC AACATGCTCT 
CCGACTCTGA ATGGTCCACG GCCACCACGT TCCCTCTCTT GCCCCATCTC TGCAGGTCTT 
CTGCCTCAGC ACACCCCACC TGGAGTGGCC ATACATACAG AGACTCCTAA ACTTGCTCCA 
TTGCCTCCAA CACCACCTCC AGCTCCCCTC ATGAAGCAGA GTCCATTGAT GCCTGCGTTG 
CCCACTAGAA ATCACTGCTT CCACCGGGGA AATTTGCCAG CGCTTCCCAG CTCTGGTCTA 
AGTCCTCTTG GACAGTTGGA GCCTGCACCG CAGGAAGAAC CAGTAAAGGT TTTACCGGCA 
TGCGTGACAC AGCGACAGCC TAATGGTCAG TCATTGAGGC AGCTGCCGCA GTGCTACAGC 
GACTTCCTGC CTGATTATTT CTCTCAGACA GAGCGCCCTC CTGAGGAGTT CTGCTTATCT 
CCTGACGCTC ATACTGATGA ATCCATCTCT ATAGACCTGT TGCAGAAAAG AGGTTTGGTG 
AAAGCCATCA ACACTGCAGT CGATCTGATA GTTGCTCATT TTGGCACCAG TCGGGATGCA 
GGTGTTAAGG CCAAATTGGG TAATAGCTCA GTTAGTCCGA ATGTGGGTCA CCTTATTCTG 
AAGTACTTGT GTCCGGCCAT TCGGGAGATA CTGAATGATG GACTGAAAGC ATACGTACTG 
GACCTGATCA TTGGACAACG CAAAAACCAG CCCTGGAGTG TGGTTGAAGC CTCCACACAA 
CTTGGACCTT CTACACGAGT TTTGCACAGC CTGTTTTCTA AAGTGAGCCA GTATTCTGAG 
CTGACCAATC ACAGTATGAG ACTCAATGCC TTCATCTTTG GCCTGCTAAA CTTGCGATCT 
CTGGAATTCT GGTTCAATCA CATTTATACG CATGAAGACA TCATAGCAGC ACATTACCAC 
CCATGGGGTT TCCTACCGCT GTCTCAAGGA GCTTGCCAGC CTCTTTTTGA AGAGTTGATG 
CTCTTGCTTC AGCCACTGTC ACTGCTTCCC TTTGACCTTG ACCTGCTATT TGAGCCACAC 
CAGCTTCAGA AAGGAGAAGA ACACCTGAGA CGCAAAGAAC AGCTGTGCTC TGCTCGCCAG 
AGCCTCGACC AATCAGATCG CTCCACTTTC CAACTCATGA GAGGCTGTGG AATGTTGGAG 
TCTCAGGAAA CGGGAATGCT GCCTCAGAGA GAAAGCTTCG TGCTGAGAGA TGAGGAGTGC 
CGGCTTAGGA CTGAGGGAGT GACATTAAAA ATGAAACAAG AATGGCAGAG GAGTGTGGGA 
GCAGAACGTG AGTTTCGGAA TAAGGAGGAT GGAGTTGGTA AAGAGTGCTT GCGAAAGAGT 
GACACAGGAC AGATGCAAGG TGGAGGGTGC TGTGCTGCCA GAATGAAGGG AGTTGGAGAA 
GACAATGAGA AGGACAAAAA GAGAGAAAAA GATGGAGATG CAACCAAACA GAGAAACCGA 
CAAGCTGGCT GGTGGTATCA GTTGATGCAA AGCTCACAGG TTTACATTGA CAACTCTGCC 
GAGGGGTCGA AATTTGTCAA GTGGGAGAAG AGGAGGAAAG GAGGGGTTGA GAATCACCGT 
CAAAGCCATC CACCTCCTAG AGAAGGTGTT GTTGAAGGAG CTGAGGCCAT TCAGCAGATG 
GATGAACAGG TGGAACACGG TCGGACCAGT AGTAGCAATA TTAATAGCAT TGGCCGTGGG 
GTTCTAAACC AATCAGCTTT GTCAGAACCC ACCAAAGCCA CAAAAGCGAA GCCATCTTGG 
ATGGGAAGTC CACCGGAGTC AGTGCTCACT GAGCTGAAAA AGAGTAAGGA GAAGCAGCCA 
GATTGCCAGG ATCCGAATGA AGGGCTTCAG GCCCAGCCAG AAGCAGAGGA GGATGGAACG 
GCTCAAGTGC TCCGCTGGGG ACGACTATTT GGAGCTGGAA ATGCAAGCAA GAAAGAAAAG 
ATTGAACAGA AATCAGTCAG GAAACAAAGA TTGCCTTCAG GTTGGTTGAG TATCGATCAT 
TCTGTGTTGG ATCTGGTGGC TCAGTCTGTT GGAGTTCGGA AAAGAGCGGA GCAACAATCT 
TTTACATCTC AAAGCCAAGA ATCCAACCTT GGCCCAACTG AACAAGCACA AACACAGAGT 
CCCCAAACAA AGACACTAGC CCCACGGGAA GTGAAGGCGT TATGCCATCA TATTGCCACA 
GAGCCAGGGC AGCTCAGTTT CAACAAAGGT GACGTGCTGC AGGTGCTCAG CAGGGCAGAC 
TCTGATTGGC TGATGTGTGC TCTGGGCGAT AGCCAGGGCC TTGTTCCCAT CATATACGTC 
ACCCTGATTG AAGACAGTCA GGAGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165250.1
CDS	303..2792
Translation	MQSRGAAAPEGDEDDSEEHHRPAHGTSNTPTTVRPSPLGSYSPIRLQGAPTSSGNCSTCSPTLNGPRPPRSLSCPISAGLLPQHTPPGVAIHTETPKLAPLPPTPPPAPLMKQSPLMPALPTRNHCFHRGNLPALPSSGLSPLGQLEPAPQEEPVKVLPACVTQRQPNGQSLRQLPQCYSDFLPDYFSQTERPPEEFCLSPDAHTDESISIDLLQKRGLVKAINTAVDLIVAHFGTSRDAGVKAKLGNSSVSPNVGHLILKYLCPAIREILNDGLKAYVLDLIIGQRKNQPWSVVEASTQLGPSTRVLHSLFSKVSQYSELTNHSMRLNAFIFGLLNLRSLEFWFNHIYTHEDIIAAHYHPWGFLPLSQGACQPLFEELMLLLQPLSLLPFDLDLLFEPHQLQKGEEHLRRKEQLCSARQSLDQSDRSTFQLMRGCGMLESQETGMLPQRESFVLRDEECRLRTEGVTLKMKQEWQRSVGAEREFRNKEDGVGKECLRKSDTGQMQGGGCCAARMKGVGEDNEKDKKREKDGDATKQRNRQAGWWYQLMQSSQVYIDNSAEGSKFVKWEKRRKGGVENHRQSHPPPREGVVEGAEAIQQMDEQVEHGRTSSSNINSIGRGVLNQSALSEPTKATKAKPSWMGSPPESVLTELKKSKEKQPDCQDPNEGLQAQPEAEEDGTAQVLRWGRLFGAGNASKKEKIEQKSVRKQRLPSGWLSIDHSVLDLVAQSVGVRKRAEQQSFTSQSQESNLGPTEQAQTQSPQTKTLAPREVKALCHHIATEPGQLSFNKGDVLQVLSRADSDWLMCALGDSQGLVPIIYVTLIEDSQER

Target ORF information:

RefSeq Version	XM_005165193.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165193.3

ORF Insert Sequence:

ATGCAGAGTC GAGGGGCTGC AGCACCAGAG GGTGATGAAG ATGACTCTGA AGAACACCAC 
AGGCCAGCTC ACGGCACCTC CAACACACCT ACAACAGTGC GGCCATCTCC ACTGGGAAGC 
TATTCCCCAA TTCGGCTCCA GGGTGCACCT ACCTCTTCTG GAAATTGCTC AACATGCTCT 
CCGACTCTGA ATGGTCCACG GCCACCACGT TCCCTCTCTT GCCCCATCTC TGCAGGTCTT 
CTGCCTCAGC ACACCCCACC TGGAGTGGCC ATACATACAG AGACTCCTAA ACTTGCTCCA 
TTGCCTCCAA CACCACCTCC AGCTCCCCTC ATGAAGCAGA GTCCATTGAT GCCTGCGTTG 
CCCACTAGAA ATCACTGCTT CCACCGGGGA AATTTGCCAG CGCTTCCCAG CTCTGGTCTA 
AGTCCTCTTG GACAGTTGGA GCCTGCACCG CAGGAAGAAC CAGTAAAGGT TTTACCGGCA 
TGCGTGACAC AGCGACAGCC TAATGGTCAG TCATTGAGGC AGCTGCCGCA GTGCTACAGC 
GACTTCCTGC CTGATTATTT CTCTCAGACA GAGCGCCCTC CTGAGGAGTT CTGCTTATCT 
CCTGACGCTC ATACTGATGA ATCCATCTCT ATAGACCTGT TGCAGAAAAG AGGTTTGGTG 
AAAGCCATCA ACACTGCAGT CGATCTGATA GTTGCTCATT TTGGCACCAG TCGGGATGCA 
GGTGTTAAGG CCAAATTGGG TAATAGCTCA GTTAGTCCGA ATGTGGGTCA CCTTATTCTG 
AAGTACTTGT GTCCGGCCAT TCGGGAGATA CTGAATGATG GACTGAAAGC ATACGTACTG 
GACCTGATCA TTGGACAACG CAAAAACCAG CCCTGGAGTG TGGTTGAAGC CTCCACACAA 
CTTGGACCTT CTACACGAGT TTTGCACAGC CTGTTTTCTA AAGTGAGCCA GTATTCTGAG 
CTGACCAATC ACAGTATGAG ACTCAATGCC TTCATCTTTG GCCTGCTAAA CTTGCGATCT 
CTGGAATTCT GGTTCAATCA CATTTATACG CATGAAGACA TCATAGCAGC ACATTACCAC 
CCATGGGGTT TCCTACCGCT GTCTCAAGGA GCTTGCCAGC CTCTTTTTGA AGAGTTGATG 
CTCTTGCTTC AGCCACTGTC ACTGCTTCCC TTTGACCTTG ACCTGCTATT TGAGCCACAC 
CAGCTTCAGA AAGGAGAAGA ACACCTGAGA CGCAAAGAAC AGCTGTGCTC TGCTCGCCAG 
AGCCTCGACC AATCAGATCG CTCCACTTTC CAACTCATGA GAGGCTGTGG AATGTTGGAG 
TCTCAGGAAA CGGGAATGCT GCCTCAGAGA GAAAGCTTCG TGCTGAGAGA TGAGGAGTGC 
CGGCTTAGGA CTGAGGGAGT GACATTAAAA ATGAAACAAG AATGGCAGAG GAGTGTGGGA 
GCAGAACGTG AGTTTCGGAA TAAGGAGGAT GGAGTTGGTA AAGAGTGCTT GCGAAAGAGT 
GACACAGGAC AGATGCAAGG TGGAGGGTGC TGTGCTGCCA GAATGAAGGG AGTTGGAGAA 
GACAATGAGA AGGACAAAAA GAGAGAAAAA GATGGAGATG CAACCAAACA GAGAAACCGA 
CAAGCTGGCT GGTGGTATCA GTTGATGCAA AGCTCACAGG TTTACATTGA CAACTCTGCC 
GAGGGGTCGA AATTTGTCAA GTGGGAGAAG AGGAGGAAAG GAGGGGTTGA GAATCACCGT 
CAAAGCCATC CACCTCCTAG AGAAGGTGTT GTTGAAGGAG CTGAGGCCAT TCAGCAGATG 
GATGAACAGG TGGAACACGG TCGGACCAGT AGTAGCAATA TTAATAGCAT TGGCCGTGGG 
GTTCTAAACC AATCAGCTTT GTCAGAACCC ACCAAAGCCA CAAAAGCGAA GCCATCTTGG 
ATGGGAAGTC CACCGGAGTC AGTGCTCACT GAGCTGAAAA AGAGTAAGGA GAAGCAGCCA 
GATTGCCAGG ATCCGAATGA AGGGCTTCAG GCCCAGCCAG AAGCAGAGGA GGATGGAACG 
GCTCAAGTGC TCCGCTGGGG ACGACTATTT GGAGCTGGAA ATGCAAGCAA GAAAGAAAAG 
ATTGAACAGA AATCAGTCAG GAAACAAAGA TTGCCTTCAG GTTGGTTGAG TATCGATCAT 
TCTGTGTTGG ATCTGGTGGC TCAGTCTGTT GGAGTTCGGA AAAGAGCGGA GCAACAATCT 
TTTACATCTC AAAGCCAAGA ATCCAACCTT GGCCCAACTG AACAAGCACA AACACAGAGT 
CCCCAAACAA AGACACTAGC CCCACGGGAA GTGAAGGCGT TATGCCATCA TATTGCCACA 
GAGCCAGGGC AGCTCAGTTT CAACAAAGGT GACGTGCTGC AGGTGCTCAG CAGGGCAGAC 
TCTGATTGGC TGATGTGTGC TCTGGGCGAT AGCCAGGGCC TTGTTCCCAT CATATACGTC 
ACCCTGATTG AAGACAGTCA GGAGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_005165190.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165247.1 Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10309
Clone ID Related Accession (Same CDS sequence)	XM_005165190.3 , XM_005165190.4
Accession Version	XM_005165190.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	iporin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTC CACCTAAGCT TGCAGGAGAG ACTCTTATTG TCCACCACAT TCCGCTGGTT 
CACTGCCAGG TTTCTAGTTC ACGGAAGTGC TGTGGTAGTT CATTAAAAAG GAACAGTAAT 
CCTTTCAGCT GTCCGGAGAA CCTGGGATTG AGTCGGACGA CCTCCCTACC TGAGAGAGAC 
ATTCTTCAGC GGGAGGCACT GGTGTACAGC AGTCTCATAC AGACCTCAAG CAGCAGCCTC 
AGCTCTAGTG ACACTTTTGG GGATGGGGGG ATGAGAGTAG GAGAGAAACG AGGAAGGGGA 
GGAAGGGAAG GGAGTATGGC AAGTGACACT TCCTCTTTCA CCTCAAGCAA TTCTGAAGAT 
CATGCTCAAA GCCTTTCGAT GAAGACACAT GAACGGCCAA GGCGCAACCC CTTTCTTCTC 
AACACTGAAG ATGAAGATGA TGAGGATGAA GACAATCTAA ATGGATATTT GGAAGACTCC 
TCTTTTCACT TGCATGGAAA TTTGCATGAA ATCTCGAATG CAATGCTTTC AAATGAAGAC 
CTGCCACCTT TCCACCTACA TGACCTTGGG TTCACTGCTG AACCTTTTCT CTTGCATGAG 
TCTACAGAAA AACAGTGGTG TTCAACAAAT GGACCAAGTA GGGAAGCAGA GAGTAGAGAC 
CATAAAAGTG GGACTTTTGA TTTGTCTACT CACATGGAGG GTCTGAACAT GCTGAGATTA 
GATAGCCAGA GACGCCATGG AAGCAGTGGT TCAACCCTTT CTATGGACTG TGGTGAGCAG 
GAATGGGGTG AGGATGATGA TTATGAGGAC CACTCCATGC AGGGGGGAAG AGGCAGCTCA 
GAGTCTTCCA GTCTGGCAGC ACCACACTGC TCATGCTGTG GCCTTTCCCA ACCATACTCT 
GAACCTTTTC AGGAATTTTC TGAGTGTCAC CAGGGCTATG GCAGTGATTC CTCCTGCAAT 
AGCTCAGATG GAGTACTGGT TAACTTCAGC ACGATTTACA ACAAGATGAA CAATGTTGTC 
CCAGCTAAGC AGTCCCTAAA TATCAACAGT TCCACTGAGC AATCCTGTGC CTCATCTGTG 
TCTGAGCTTG TTGGGATGTG CGGGACAGAA TGTAGCAGTG GACGTGGCGC TTTCTACTTA 
GACCTTCACA CCTCCCCAGT CGAACCCCCT CAGCATCCTT TAGCCACACA AGAACATGCA 
AACTCTTCCT CTATATCTCA GCAACAAGGT GCCACTTTGG AAGTAGATGC CAACTGCAAC 
TCCTACCATA ACATTCTTGG AAGTGAGGGA CTGTCGTCTG CTGAAACCTC TGCCAATGAC 
CTCACTTCGT GTCTTCAAAG CCAAGCTCGC TTGGTTGTAG CAACACAGAA TTACTACAAG 
CTTGTGACAT GTGACCTTTC TTCCCAGTCC TCTCCCAGTC CTGTGGGATC ATCGGTCACA 
AGCTGTTCAG ATGAGCACAG CAAAGGGAGT CCCGCTCAAC CAACAGAGTA CTACTTATTT 
CAGCAGATGA AAGAGGATGA AGAAGAGGAA GACACAGAAC TGAATGAAGA AAATGACCCA 
ACTGACATTG ACTCTATTCA TGAGAATGTA ATTGAGGGGC AGGTTTATAT CAACATCTCT 
CCACCTACAA CAGCCTGTAA TTCAGTAGCT GCTTCGGGGA GCAGTCGACC TCGCTCTCGA 
AGCTATGATC GGAACTTGGA CAAGTCTCCA TCTCCTCGTC TTGGATCCTT GGAGCGTATG 
CTCAGCTGCC CTGTTCGGCT CAGTGAGAGT GCAGACCCAA GCCCGCCACC TCCACCACGT 
GTTACTTCCT TTGCTGAAAT TGCAAGGAAT AAGAGAAGAA CCGGAGGATC GCCATCTCAG 
AGGAGTGGGA TCGAGGCCAC GTCCACACAC TCTCATTCCT CTGGAGAGTT CTCGCCCATC 
CTGGAAGGCC TTCCTGAGGG TCAGAGCCAT AGCTTGCCAC CTCTGACTCG ATGTCATAGT 
CAGGGGAGCT GTGATTTGTC CTCTTCATAT AGACCATGGA GTACGCCACT GCGAGAATCT 
CCAGGGGGAA TGGGAAGACA GACTCGATCC AAAGCAGAAG GTGGCCTGTC TAGCTCGACG 
GACTCCTCTC CAACTGTAAT TCGCTATAGC AAAGATCAAC GCCCAACCTC TTTACCTATC 
CAGCCCTTCA CTTTTCAGCA CCAGTTTGGG AAACCCCAGG CAAAGCCTAT CCTTCCTCTT 
CTGGACGGAT ACATAAGCCA AATGCAGAGT CGAGGGGCTG CAGCACCAGA GGGTGATGAA 
GATGACTCTG AAGAACACCA CAGGCCAGCT CACGGCACCT CCAACACACC TACAACAGTG 
CGGCCATCTC CACTGGGAAG CTATTCCCCA ATTCGGCTCC AGGGTGCACC TACCTCTTCT 
GGAAATTGCT CAACATGCTC TCCGACTCTG AATGGTCCAC GGCCACCACG TTCCCTCTCT 
TGCCCCATCT CTGCAGGTCT TCTGCCTCAG CACACCCCAC CTGGAGTGGC CATACATACA 
GAGACTCCTA AACTTGCTCC ATTGCCTCCA ACACCACCTC CAGCTCCCCT CATGAAGCAG 
AGTCCATTGA TGCCTGCGTT GCCCACTAGA AATCACTGCT TCCACCGGGG AAATTTGCCA 
GCGCTTCCCA GCTCTGGTCT AAGTCCTCTT GGACAGTTGG AGCCTGCACC GCAGGAAGAA 
CCAGTAAAGG TTTTACCGGC ATGCGTGACA CAGCGACAGC CTAATGTGCA CCACCTCTCC 
CCACAGGCAC TCAAATGGAG AGAGTACAGG CGCAAGAACC CTCTGGGTGT GGAGCGCTGT 
AAAGATGGCC CTCATTCTTT CTCTTGTTCT TTGGAAACCA AGAGACCTGG GACTCGTGTC 
ATGCGGCGCA ATGTCTTTGA TTTCCCACCA ACCAATCAAG CACTCAGCTT CGGCAGACTG 
AATGGTCAGT CATTGAGGCA GCTGCCGCAG TGCTACAGCG ACTTCCTGCC TGATTATTTC 
TCTCAGACAG AGCGCCCTCC TGAGGAGTTC TGCTTATCTC CTGACGCTCA TACTGATGAA 
TCCATCTCTA TAGACCTGTT GCAGAAAAGA GGTTTGGTGA AAGCCATCAA CACTGCAGTC 
GATCTGATAG TTGCTCATTT TGGCACCAGT CGGGATGCAG GTGTTAAGGC CAAATTGGGT 
AATAGCTCAG TTAGTCCGAA TGTGGGTCAC CTTATTCTGA AGTACTTGTG TCCGGCCATT 
CGGGAGATAC TGAATGATGG ACTGAAAGCA TACGTACTGG ACCTGATCAT TGGACAACGC 
AAAAACCAGC CCTGGAGTGT GGTTGAAGCC TCCACACAAC TTGGACCTTC TACACGAGTT 
TTGCACAGCC TGTTTTCTAA AGTGAGCCAG TATTCTGAGC TGACCAATCA CAGTATGAGA 
CTCAATGCCT TCATCTTTGG CCTGCTAAAC TTGCGATCTC TGGAATTCTG GTTCAATCAC 
ATTTATACGC ATGAAGACAT CATAGCAGCA CATTACCACC CATGGGGTTT CCTACCGCTG 
TCTCAAGGAG CTTGCCAGCC TCTTTTTGAA GAGTTGATGC TCTTGCTTCA GCCACTGTCA 
CTGCTTCCCT TTGACCTTGA CCTGCTATTT GAGCCACACC AGCTTCAGAA AGGAGAAGAA 
CACCTGAGAC GCAAAGAACA GCTGTGCTCT GCTCGCCAGA GCCTCGACCA ATCAGATCGC 
TCCACTTTCC AACTCATGAG AGGCTGTGGA ATGTTGGAGT CTCAGGAAAC GGGAATGCTG 
CCTCAGAGAG AAAGCTTCGT GCTGAGAGAT GAGGAGTGCC GGCTTAGGAC TGAGGGAGTG 
ACATTAAAAA TGAAACAAGA ATGGCAGAGG AGTGTGGGAG CAGAACGTGA GTTTCGGAAT 
AAGGAGGATG GAGTTGGTAA AGAGTGCTTG CGAAAGAGTG ACACAGGACA GATGCAAGGT 
GGAGGGTGCT GTGCTGCCAG AATGAAGGGA GTTGGAGAAG ACAATGAGAA GGACAAAAAG 
AGAGAAAAAG ATGGAGATGC AACCAAACAG AGAAACCGAC AAGCTGGCTG GTGGTATCAG 
TTGATGCAAA GCTCACAGGT TTACATTGAC AACTCTGCCG AGGGGTCGAA ATTTGTCAAG 
TGGGAGAAGA GGAGGAAAGG AGGGGTTGAG AATCACCGTC AAAGCCATCC ACCTCCTAGA 
GAAGGTGTTG TTGAAGGAGC TGAGGCCATT CAGCAGATGG ATGAACAGGT GGAACACGGT 
CGGACCAGTA GTAGCAATAT TAATAGCATT GGCCGTGGGG TTCTAAACCA ATCAGCTTTG 
TCAGAACCCA CCAAAGCCAC AAAAGCGAAG CCATCTTGGA TGGGAAGTCC ACCGGAGTCA 
GTGCTCACTG AGCTGAAAAA GAGTAAGGAG AAGCAGCCAG ATTGCCAGGA TCCGAATGAA 
GGGCTTCAGG CCCAGCCAGA AGCAGAGGAG GATGGAACGG CTCAAGTGCT CCGCTGGGGA 
CGACTATTTG GAGCTGGAAA TGCAAGCAAG AAAGAAAAGA TTGAACAGAA ATCAGTCAGG 
AAACAAAGAT TGCCTTCAGG TTGGTTGAGT ATCGATCATT CTGTGTTGGA TCTGGTGGCT 
CAGTCTGTTG GAGTTCGGAA AAGAGCGGAG CAACAATCTT TTACATCTCA AAGCCAAGAA 
TCCAACCTTG GCCCAACTGA ACAAGCACAA ACACAGAGTC CCCAAACAAA GACACTAGCC 
CCACGGGAAG TGAAGGCGTT ATGCCATCAT ATTGCCACAG AGCCAGGGCA GCTCAGTTTC 
AACAAAGGTG ACGTGCTGCA GGTGCTCAGC AGGGCAGACT CTGATTGGCT GATGTGTGCT 
CTGGGCGATA GCCAGGGCCT TGTTCCCATC ATATACGTCA CCCTGATTGA AGACAGTCAG 
GAGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165247.1
CDS	494..5422
Translation	MDSPPKLAGETLIVHHIPLVHCQVSSSRKCCGSSLKRNSNPFSCPENLGLSRTTSLPERDILQREALVYSSLIQTSSSSLSSSDTFGDGGMRVGEKRGRGGREGSMASDTSSFTSSNSEDHAQSLSMKTHERPRRNPFLLNTEDEDDEDEDNLNGYLEDSSFHLHGNLHEISNAMLSNEDLPPFHLHDLGFTAEPFLLHESTEKQWCSTNGPSREAESRDHKSGTFDLSTHMEGLNMLRLDSQRRHGSSGSTLSMDCGEQEWGEDDDYEDHSMQGGRGSSESSSLAAPHCSCCGLSQPYSEPFQEFSECHQGYGSDSSCNSSDGVLVNFSTIYNKMNNVVPAKQSLNINSSTEQSCASSVSELVGMCGTECSSGRGAFYLDLHTSPVEPPQHPLATQEHANSSSISQQQGATLEVDANCNSYHNILGSEGLSSAETSANDLTSCLQSQARLVVATQNYYKLVTCDLSSQSSPSPVGSSVTSCSDEHSKGSPAQPTEYYLFQQMKEDEEEEDTELNEENDPTDIDSIHENVIEGQVYINISPPTTACNSVAASGSSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSESADPSPPPPPRVTSFAEIARNKRRTGGSPSQRSGIEATSTHSHSSGEFSPILEGLPEGQSHSLPPLTRCHSQGSCDLSSSYRPWSTPLRESPGGMGRQTRSKAEGGLSSSTDSSPTVIRYSKDQRPTSLPIQPFTFQHQFGKPQAKPILPLLDGYISQMQSRGAAAPEGDEDDSEEHHRPAHGTSNTPTTVRPSPLGSYSPIRLQGAPTSSGNCSTCSPTLNGPRPPRSLSCPISAGLLPQHTPPGVAIHTETPKLAPLPPTPPPAPLMKQSPLMPALPTRNHCFHRGNLPALPSSGLSPLGQLEPAPQEEPVKVLPACVTQRQPNVHHLSPQALKWREYRRKNPLGVERCKDGPHSFSCSLETKRPGTRVMRRNVFDFPPTNQALSFGRLNGQSLRQLPQCYSDFLPDYFSQTERPPEEFCLSPDAHTDESISIDLLQKRGLVKAINTAVDLIVAHFGTSRDAGVKAKLGNSSVSPNVGHLILKYLCPAIREILNDGLKAYVLDLIIGQRKNQPWSVVEASTQLGPSTRVLHSLFSKVSQYSELTNHSMRLNAFIFGLLNLRSLEFWFNHIYTHEDIIAAHYHPWGFLPLSQGACQPLFEELMLLLQPLSLLPFDLDLLFEPHQLQKGEEHLRRKEQLCSARQSLDQSDRSTFQLMRGCGMLESQETGMLPQRESFVLRDEECRLRTEGVTLKMKQEWQRSVGAEREFRNKEDGVGKECLRKSDTGQMQGGGCCAARMKGVGEDNEKDKKREKDGDATKQRNRQAGWWYQLMQSSQVYIDNSAEGSKFVKWEKRRKGGVENHRQSHPPPREGVVEGAEAIQQMDEQVEHGRTSSSNINSIGRGVLNQSALSEPTKATKAKPSWMGSPPESVLTELKKSKEKQPDCQDPNEGLQAQPEAEEDGTAQVLRWGRLFGAGNASKKEKIEQKSVRKQRLPSGWLSIDHSVLDLVAQSVGVRKRAEQQSFTSQSQESNLGPTEQAQTQSPQTKTLAPREVKALCHHIATEPGQLSFNKGDVLQVLSRADSDWLMCALGDSQGLVPIIYVTLIEDSQER

Target ORF information:

RefSeq Version	XM_005165190.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165190.3

ORF Insert Sequence:

ATGGACAGTC CACCTAAGCT TGCAGGAGAG ACTCTTATTG TCCACCACAT TCCGCTGGTT 
CACTGCCAGG TTTCTAGTTC ACGGAAGTGC TGTGGTAGTT CATTAAAAAG GAACAGTAAT 
CCTTTCAGCT GTCCGGAGAA CCTGGGATTG AGTCGGACGA CCTCCCTACC TGAGAGAGAC 
ATTCTTCAGC GGGAGGCACT GGTGTACAGC AGTCTCATAC AGACCTCAAG CAGCAGCCTC 
AGCTCTAGTG ACACTTTTGG GGATGGGGGG ATGAGAGTAG GAGAGAAACG AGGAAGGGGA 
GGAAGGGAAG GGAGTATGGC AAGTGACACT TCCTCTTTCA CCTCAAGCAA TTCTGAAGAT 
CATGCTCAAA GCCTTTCGAT GAAGACACAT GAACGGCCAA GGCGCAACCC CTTTCTTCTC 
AACACTGAAG ATGAAGATGA TGAGGATGAA GACAATCTAA ATGGATATTT GGAAGACTCC 
TCTTTTCACT TGCATGGAAA TTTGCATGAA ATCTCGAATG CAATGCTTTC AAATGAAGAC 
CTGCCACCTT TCCACCTACA TGACCTTGGG TTCACTGCTG AACCTTTTCT CTTGCATGAG 
TCTACAGAAA AACAGTGGTG TTCAACAAAT GGACCAAGTA GGGAAGCAGA GAGTAGAGAC 
CATAAAAGTG GGACTTTTGA TTTGTCTACT CACATGGAGG GTCTGAACAT GCTGAGATTA 
GATAGCCAGA GACGCCATGG AAGCAGTGGT TCAACCCTTT CTATGGACTG TGGTGAGCAG 
GAATGGGGTG AGGATGATGA TTATGAGGAC CACTCCATGC AGGGGGGAAG AGGCAGCTCA 
GAGTCTTCCA GTCTGGCAGC ACCACACTGC TCATGCTGTG GCCTTTCCCA ACCATACTCT 
GAACCTTTTC AGGAATTTTC TGAGTGTCAC CAGGGCTATG GCAGTGATTC CTCCTGCAAT 
AGCTCAGATG GAGTACTGGT TAACTTCAGC ACGATTTACA ACAAGATGAA CAATGTTGTC 
CCAGCTAAGC AGTCCCTAAA TATCAACAGT TCCACTGAGC AATCCTGTGC CTCATCTGTG 
TCTGAGCTTG TTGGGATGTG CGGGACAGAA TGTAGCAGTG GACGTGGCGC TTTCTACTTA 
GACCTTCACA CCTCCCCAGT CGAACCCCCT CAGCATCCTT TAGCCACACA AGAACATGCA 
AACTCTTCCT CTATATCTCA GCAACAAGGT GCCACTTTGG AAGTAGATGC CAACTGCAAC 
TCCTACCATA ACATTCTTGG AAGTGAGGGA CTGTCGTCTG CTGAAACCTC TGCCAATGAC 
CTCACTTCGT GTCTTCAAAG CCAAGCTCGC TTGGTTGTAG CAACACAGAA TTACTACAAG 
CTTGTGACAT GTGACCTTTC TTCCCAGTCC TCTCCCAGTC CTGTGGGATC ATCGGTCACA 
AGCTGTTCAG ATGAGCACAG CAAAGGGAGT CCCGCTCAAC CAACAGAGTA CTACTTATTT 
CAGCAGATGA AAGAGGATGA AGAAGAGGAA GACACAGAAC TGAATGAAGA AAATGACCCA 
ACTGACATTG ACTCTATTCA TGAGAATGTA ATTGAGGGGC AGGTTTATAT CAACATCTCT 
CCACCTACAA CAGCCTGTAA TTCAGTAGCT GCTTCGGGGA GCAGTCGACC TCGCTCTCGA 
AGCTATGATC GGAACTTGGA CAAGTCTCCA TCTCCTCGTC TTGGATCCTT GGAGCGTATG 
CTCAGCTGCC CTGTTCGGCT CAGTGAGAGT GCAGACCCAA GCCCGCCACC TCCACCACGT 
GTTACTTCCT TTGCTGAAAT TGCAAGGAAT AAGAGAAGAA CCGGAGGATC GCCATCTCAG 
AGGAGTGGGA TCGAGGCCAC GTCCACACAC TCTCATTCCT CTGGAGAGTT CTCGCCCATC 
CTGGAAGGCC TTCCTGAGGG TCAGAGCCAT AGCTTGCCAC CTCTGACTCG ATGTCATAGT 
CAGGGGAGCT GTGATTTGTC CTCTTCATAT AGACCATGGA GTACGCCACT GCGAGAATCT 
CCAGGGGGAA TGGGAAGACA GACTCGATCC AAAGCAGAAG GTGGCCTGTC TAGCTCGACG 
GACTCCTCTC CAACTGTAAT TCGCTATAGC AAAGATCAAC GCCCAACCTC TTTACCTATC 
CAGCCCTTCA CTTTTCAGCA CCAGTTTGGG AAACCCCAGG CAAAGCCTAT CCTTCCTCTT 
CTGGACGGAT ACATAAGCCA AATGCAGAGT CGAGGGGCTG CAGCACCAGA GGGTGATGAA 
GATGACTCTG AAGAACACCA CAGGCCAGCT CACGGCACCT CCAACACACC TACAACAGTG 
CGGCCATCTC CACTGGGAAG CTATTCCCCA ATTCGGCTCC AGGGTGCACC TACCTCTTCT 
GGAAATTGCT CAACATGCTC TCCGACTCTG AATGGTCCAC GGCCACCACG TTCCCTCTCT 
TGCCCCATCT CTGCAGGTCT TCTGCCTCAG CACACCCCAC CTGGAGTGGC CATACATACA 
GAGACTCCTA AACTTGCTCC ATTGCCTCCA ACACCACCTC CAGCTCCCCT CATGAAGCAG 
AGTCCATTGA TGCCTGCGTT GCCCACTAGA AATCACTGCT TCCACCGGGG AAATTTGCCA 
GCGCTTCCCA GCTCTGGTCT AAGTCCTCTT GGACAGTTGG AGCCTGCACC GCAGGAAGAA 
CCAGTAAAGG TTTTACCGGC ATGCGTGACA CAGCGACAGC CTAATGTGCA CCACCTCTCC 
CCACAGGCAC TCAAATGGAG AGAGTACAGG CGCAAGAACC CTCTGGGTGT GGAGCGCTGT 
AAAGATGGCC CTCATTCTTT CTCTTGTTCT TTGGAAACCA AGAGACCTGG GACTCGTGTC 
ATGCGGCGCA ATGTCTTTGA TTTCCCACCA ACCAATCAAG CACTCAGCTT CGGCAGACTG 
AATGGTCAGT CATTGAGGCA GCTGCCGCAG TGCTACAGCG ACTTCCTGCC TGATTATTTC 
TCTCAGACAG AGCGCCCTCC TGAGGAGTTC TGCTTATCTC CTGACGCTCA TACTGATGAA 
TCCATCTCTA TAGACCTGTT GCAGAAAAGA GGTTTGGTGA AAGCCATCAA CACTGCAGTC 
GATCTGATAG TTGCTCATTT TGGCACCAGT CGGGATGCAG GTGTTAAGGC CAAATTGGGT 
AATAGCTCAG TTAGTCCGAA TGTGGGTCAC CTTATTCTGA AGTACTTGTG TCCGGCCATT 
CGGGAGATAC TGAATGATGG ACTGAAAGCA TACGTACTGG ACCTGATCAT TGGACAACGC 
AAAAACCAGC CCTGGAGTGT GGTTGAAGCC TCCACACAAC TTGGACCTTC TACACGAGTT 
TTGCACAGCC TGTTTTCTAA AGTGAGCCAG TATTCTGAGC TGACCAATCA CAGTATGAGA 
CTCAATGCCT TCATCTTTGG CCTGCTAAAC TTGCGATCTC TGGAATTCTG GTTCAATCAC 
ATTTATACGC ATGAAGACAT CATAGCAGCA CATTACCACC CATGGGGTTT CCTACCGCTG 
TCTCAAGGAG CTTGCCAGCC TCTTTTTGAA GAGTTGATGC TCTTGCTTCA GCCACTGTCA 
CTGCTTCCCT TTGACCTTGA CCTGCTATTT GAGCCACACC AGCTTCAGAA AGGAGAAGAA 
CACCTGAGAC GCAAAGAACA GCTGTGCTCT GCTCGCCAGA GCCTCGACCA ATCAGATCGC 
TCCACTTTCC AACTCATGAG AGGCTGTGGA ATGTTGGAGT CTCAGGAAAC GGGAATGCTG 
CCTCAGAGAG AAAGCTTCGT GCTGAGAGAT GAGGAGTGCC GGCTTAGGAC TGAGGGAGTG 
ACATTAAAAA TGAAACAAGA ATGGCAGAGG AGTGTGGGAG CAGAACGTGA GTTTCGGAAT 
AAGGAGGATG GAGTTGGTAA AGAGTGCTTG CGAAAGAGTG ACACAGGACA GATGCAAGGT 
GGAGGGTGCT GTGCTGCCAG AATGAAGGGA GTTGGAGAAG ACAATGAGAA GGACAAAAAG 
AGAGAAAAAG ATGGAGATGC AACCAAACAG AGAAACCGAC AAGCTGGCTG GTGGTATCAG 
TTGATGCAAA GCTCACAGGT TTACATTGAC AACTCTGCCG AGGGGTCGAA ATTTGTCAAG 
TGGGAGAAGA GGAGGAAAGG AGGGGTTGAG AATCACCGTC AAAGCCATCC ACCTCCTAGA 
GAAGGTGTTG TTGAAGGAGC TGAGGCCATT CAGCAGATGG ATGAACAGGT GGAACACGGT 
CGGACCAGTA GTAGCAATAT TAATAGCATT GGCCGTGGGG TTCTAAACCA ATCAGCTTTG 
TCAGAACCCA CCAAAGCCAC AAAAGCGAAG CCATCTTGGA TGGGAAGTCC ACCGGAGTCA 
GTGCTCACTG AGCTGAAAAA GAGTAAGGAG AAGCAGCCAG ATTGCCAGGA TCCGAATGAA 
GGGCTTCAGG CCCAGCCAGA AGCAGAGGAG GATGGAACGG CTCAAGTGCT CCGCTGGGGA 
CGACTATTTG GAGCTGGAAA TGCAAGCAAG AAAGAAAAGA TTGAACAGAA ATCAGTCAGG 
AAACAAAGAT TGCCTTCAGG TTGGTTGAGT ATCGATCATT CTGTGTTGGA TCTGGTGGCT 
CAGTCTGTTG GAGTTCGGAA AAGAGCGGAG CAACAATCTT TTACATCTCA AAGCCAAGAA 
TCCAACCTTG GCCCAACTGA ACAAGCACAA ACACAGAGTC CCCAAACAAA GACACTAGCC 
CCACGGGAAG TGAAGGCGTT ATGCCATCAT ATTGCCACAG AGCCAGGGCA GCTCAGTTTC 
AACAAAGGTG ACGTGCTGCA GGTGCTCAGC AGGGCAGACT CTGATTGGCT GATGTGTGCT 
CTGGGCGATA GCCAGGGCCT TGTTCCCATC ATATACGTCA CCCTGATTGA AGACAGTCAG 
GAGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_005165190.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165247.1 Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10309
Clone ID Related Accession (Same CDS sequence)	XM_005165190.3 , XM_005165190.4
Accession Version	XM_005165190.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165190.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTC CACCTAAGCT TGCAGGAGAG ACTCTTATTG TCCACCACAT TCCGCTGGTT 
CACTGCCAGG TTTCTAGTTC ACGGAAGTGC TGTGGTAGTT CATTAAAAAG GAACAGTAAT 
CCTTTCAGCT GTCCGGAGAA CCTGGGATTG AGTCGGACGA CCTCCCTACC TGAGAGAGAC 
ATTCTTCAGC GGGAGGCACT GGTGTACAGC AGTCTCATAC AGACCTCAAG CAGCAGCCTC 
AGCTCTAGTG ACACTTTTGG GGATGGGGGG ATGAGAGTAG GAGAGAAACG AGGAAGGGGA 
GGAAGGGAAG GGAGTATGGC AAGTGACACT TCCTCTTTCA CCTCAAGCAA TTCTGAAGAT 
CATGCTCAAA GCCTTTCGAT GAAGACACAT GAACGGCCAA GGCGCAACCC CTTTCTTCTC 
AACACTGAAG ATGAAGATGA TGAGGATGAA GACAATCTAA ATGGATATTT GGAAGACTCC 
TCTTTTCACT TGCATGGAAA TTTGCATGAA ATCTCGAATG CAATGCTTTC AAATGAAGAC 
CTGCCACCTT TCCACCTACA TGACCTTGGG TTCACTGCTG AACCTTTTCT CTTGCATGAG 
TCTACAGAAA AACAGTGGTG TTCAACAAAT GGACCAAGTA GGGAAGCAGA GAGTAGAGAC 
CATAAAAGTG GGACTTTTGA TTTGTCTACT CACATGGAGG GTCTGAACAT GCTGAGATTA 
GATAGCCAGA GACGCCATGG AAGCAGTGGT TCAACCCTTT CTATGGACTG TGGTGAGCAG 
GAATGGGGTG AGGATGATGA TTATGAGGAC CACTCCATGC AGGGGGGAAG AGGCAGCTCA 
GAGTCTTCCA GTCTGGCAGC ACCACACTGC TCATGCTGTG GCCTTTCCCA ACCATACTCT 
GAACCTTTTC AGGAATTTTC TGAGTGTCAC CAGGGCTATG GCAGTGATTC CTCCTGCAAT 
AGCTCAGATG GAGTACTGGT TAACTTCAGC ACGATTTACA ACAAGATGAA CAATGTTGTC 
CCAGCTAAGC AGTCCCTAAA TATCAACAGT TCCACTGAGC AATCCTGTGC CTCATCTGTG 
TCTGAGCTTG TTGGGATGTG CGGGACAGAA TGTAGCAGTG GACGTGGCGC TTTCTACTTA 
GACCTTCACA CCTCCCCAGT CGAACCCCCT CAGCATCCTT TAGCCACACA AGAACATGCA 
AACTCTTCCT CTATATCTCA GCAACAAGGT GCCACTTTGG AAGTAGATGC CAACTGCAAC 
TCCTACCATA ACATTCTTGG AAGTGAGGGA CTGTCGTCTG CTGAAACCTC TGCCAATGAC 
CTCACTTCGT GTCTTCAAAG CCAAGCTCGC TTGGTTGTAG CAACACAGAA TTACTACAAG 
CTTGTGACAT GTGACCTTTC TTCCCAGTCC TCTCCCAGTC CTGTGGGATC ATCGGTCACA 
AGCTGTTCAG ATGAGCACAG CAAAGGGAGT CCCGCTCAAC CAACAGAGTA CTACTTATTT 
CAGCAGATGA AAGAGGATGA AGAAGAGGAA GACACAGAAC TGAATGAAGA AAATGACCCA 
ACTGACATTG ACTCTATTCA TGAGAATGTA ATTGAGGGGC AGGTTTATAT CAACATCTCT 
CCACCTACAA CAGCCTGTAA TTCAGTAGCT GCTTCGGGGA GCAGTCGACC TCGCTCTCGA 
AGCTATGATC GGAACTTGGA CAAGTCTCCA TCTCCTCGTC TTGGATCCTT GGAGCGTATG 
CTCAGCTGCC CTGTTCGGCT CAGTGAGAGT GCAGACCCAA GCCCGCCACC TCCACCACGT 
GTTACTTCCT TTGCTGAAAT TGCAAGGAAT AAGAGAAGAA CCGGAGGATC GCCATCTCAG 
AGGAGTGGGA TCGAGGCCAC GTCCACACAC TCTCATTCCT CTGGAGAGTT CTCGCCCATC 
CTGGAAGGCC TTCCTGAGGG TCAGAGCCAT AGCTTGCCAC CTCTGACTCG ATGTCATAGT 
CAGGGGAGCT GTGATTTGTC CTCTTCATAT AGACCATGGA GTACGCCACT GCGAGAATCT 
CCAGGGGGAA TGGGAAGACA GACTCGATCC AAAGCAGAAG GTGGCCTGTC TAGCTCGACG 
GACTCCTCTC CAACTGTAAT TCGCTATAGC AAAGATCAAC GCCCAACCTC TTTACCTATC 
CAGCCCTTCA CTTTTCAGCA CCAGTTTGGG AAACCCCAGG CAAAGCCTAT CCTTCCTCTT 
CTGGACGGAT ACATAAGCCA AATGCAGAGT CGAGGGGCTG CAGCACCAGA GGGTGATGAA 
GATGACTCTG AAGAACACCA CAGGCCAGCT CACGGCACCT CCAACACACC TACAACAGTG 
CGGCCATCTC CACTGGGAAG CTATTCCCCA ATTCGGCTCC AGGGTGCACC TACCTCTTCT 
GGAAATTGCT CAACATGCTC TCCGACTCTG AATGGTCCAC GGCCACCACG TTCCCTCTCT 
TGCCCCATCT CTGCAGGTCT TCTGCCTCAG CACACCCCAC CTGGAGTGGC CATACATACA 
GAGACTCCTA AACTTGCTCC ATTGCCTCCA ACACCACCTC CAGCTCCCCT CATGAAGCAG 
AGTCCATTGA TGCCTGCGTT GCCCACTAGA AATCACTGCT TCCACCGGGG AAATTTGCCA 
GCGCTTCCCA GCTCTGGTCT AAGTCCTCTT GGACAGTTGG AGCCTGCACC GCAGGAAGAA 
CCAGTAAAGG TTTTACCGGC ATGCGTGACA CAGCGACAGC CTAATGTGCA CCACCTCTCC 
CCACAGGCAC TCAAATGGAG AGAGTACAGG CGCAAGAACC CTCTGGGTGT GGAGCGCTGT 
AAAGATGGCC CTCATTCTTT CTCTTGTTCT TTGGAAACCA AGAGACCTGG GACTCGTGTC 
ATGCGGCGCA ATGTCTTTGA TTTCCCACCA ACCAATCAAG CACTCAGCTT CGGCAGACTG 
AATGGTCAGT CATTGAGGCA GCTGCCGCAG TGCTACAGCG ACTTCCTGCC TGATTATTTC 
TCTCAGACAG AGCGCCCTCC TGAGGAGTTC TGCTTATCTC CTGACGCTCA TACTGATGAA 
TCCATCTCTA TAGACCTGTT GCAGAAAAGA GGTTTGGTGA AAGCCATCAA CACTGCAGTC 
GATCTGATAG TTGCTCATTT TGGCACCAGT CGGGATGCAG GTGTTAAGGC CAAATTGGGT 
AATAGCTCAG TTAGTCCGAA TGTGGGTCAC CTTATTCTGA AGTACTTGTG TCCGGCCATT 
CGGGAGATAC TGAATGATGG ACTGAAAGCA TACGTACTGG ACCTGATCAT TGGACAACGC 
AAAAACCAGC CCTGGAGTGT GGTTGAAGCC TCCACACAAC TTGGACCTTC TACACGAGTT 
TTGCACAGCC TGTTTTCTAA AGTGAGCCAG TATTCTGAGC TGACCAATCA CAGTATGAGA 
CTCAATGCCT TCATCTTTGG CCTGCTAAAC TTGCGATCTC TGGAATTCTG GTTCAATCAC 
ATTTATACGC ATGAAGACAT CATAGCAGCA CATTACCACC CATGGGGTTT CCTACCGCTG 
TCTCAAGGAG CTTGCCAGCC TCTTTTTGAA GAGTTGATGC TCTTGCTTCA GCCACTGTCA 
CTGCTTCCCT TTGACCTTGA CCTGCTATTT GAGCCACACC AGCTTCAGAA AGGAGAAGAA 
CACCTGAGAC GCAAAGAACA GCTGTGCTCT GCTCGCCAGA GCCTCGACCA ATCAGATCGC 
TCCACTTTCC AACTCATGAG AGGCTGTGGA ATGTTGGAGT CTCAGGAAAC GGGAATGCTG 
CCTCAGAGAG AAAGCTTCGT GCTGAGAGAT GAGGAGTGCC GGCTTAGGAC TGAGGGAGTG 
ACATTAAAAA TGAAACAAGA ATGGCAGAGG AGTGTGGGAG CAGAACGTGA GTTTCGGAAT 
AAGGAGGATG GAGTTGGTAA AGAGTGCTTG CGAAAGAGTG ACACAGGACA GATGCAAGGT 
GGAGGGTGCT GTGCTGCCAG AATGAAGGGA GTTGGAGAAG ACAATGAGAA GGACAAAAAG 
AGAGAAAAAG ATGGAGATGC AACCAAACAG AGAAACCGAC AAGCTGGCTG GTGGTATCAG 
TTGATGCAAA GCTCACAGGT TTACATTGAC AACTCTGCCG AGGGGTCGAA ATTTGTCAAG 
TGGGAGAAGA GGAGGAAAGG AGGGGTTGAG AATCACCGTC AAAGCCATCC ACCTCCTAGA 
GAAGGTGTTG TTGAAGGAGC TGAGGCCATT CAGCAGATGG ATGAACAGGT GGAACACGGT 
CGGACCAGTA GTAGCAATAT TAATAGCATT GGCCGTGGGG TTCTAAACCA ATCAGCTTTG 
TCAGAACCCA CCAAAGCCAC AAAAGCGAAG CCATCTTGGA TGGGAAGTCC ACCGGAGTCA 
GTGCTCACTG AGCTGAAAAA GAGTAAGGAG AAGCAGCCAG ATTGCCAGGA TCCGAATGAA 
GGGCTTCAGG CCCAGCCAGA AGCAGAGGAG GATGGAACGG CTCAAGTGCT CCGCTGGGGA 
CGACTATTTG GAGCTGGAAA TGCAAGCAAG AAAGAAAAGA TTGAACAGAA ATCAGTCAGG 
AAACAAAGAT TGCCTTCAGG TTGGTTGAGT ATCGATCATT CTGTGTTGGA TCTGGTGGCT 
CAGTCTGTTG GAGTTCGGAA AAGAGCGGAG CAACAATCTT TTACATCTCA AAGCCAAGAA 
TCCAACCTTG GCCCAACTGA ACAAGCACAA ACACAGAGTC CCCAAACAAA GACACTAGCC 
CCACGGGAAG TGAAGGCGTT ATGCCATCAT ATTGCCACAG AGCCAGGGCA GCTCAGTTTC 
AACAAAGGTG ACGTGCTGCA GGTGCTCAGC AGGGCAGACT CTGATTGGCT GATGTGTGCT 
CTGGGCGATA GCCAGGGCCT TGTTCCCATC ATATACGTCA CCCTGATTGA AGACAGTCAG 
GAGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165247.1
CDS	452..5380
Translation	MDSPPKLAGETLIVHHIPLVHCQVSSSRKCCGSSLKRNSNPFSCPENLGLSRTTSLPERDILQREALVYSSLIQTSSSSLSSSDTFGDGGMRVGEKRGRGGREGSMASDTSSFTSSNSEDHAQSLSMKTHERPRRNPFLLNTEDEDDEDEDNLNGYLEDSSFHLHGNLHEISNAMLSNEDLPPFHLHDLGFTAEPFLLHESTEKQWCSTNGPSREAESRDHKSGTFDLSTHMEGLNMLRLDSQRRHGSSGSTLSMDCGEQEWGEDDDYEDHSMQGGRGSSESSSLAAPHCSCCGLSQPYSEPFQEFSECHQGYGSDSSCNSSDGVLVNFSTIYNKMNNVVPAKQSLNINSSTEQSCASSVSELVGMCGTECSSGRGAFYLDLHTSPVEPPQHPLATQEHANSSSISQQQGATLEVDANCNSYHNILGSEGLSSAETSANDLTSCLQSQARLVVATQNYYKLVTCDLSSQSSPSPVGSSVTSCSDEHSKGSPAQPTEYYLFQQMKEDEEEEDTELNEENDPTDIDSIHENVIEGQVYINISPPTTACNSVAASGSSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSESADPSPPPPPRVTSFAEIARNKRRTGGSPSQRSGIEATSTHSHSSGEFSPILEGLPEGQSHSLPPLTRCHSQGSCDLSSSYRPWSTPLRESPGGMGRQTRSKAEGGLSSSTDSSPTVIRYSKDQRPTSLPIQPFTFQHQFGKPQAKPILPLLDGYISQMQSRGAAAPEGDEDDSEEHHRPAHGTSNTPTTVRPSPLGSYSPIRLQGAPTSSGNCSTCSPTLNGPRPPRSLSCPISAGLLPQHTPPGVAIHTETPKLAPLPPTPPPAPLMKQSPLMPALPTRNHCFHRGNLPALPSSGLSPLGQLEPAPQEEPVKVLPACVTQRQPNVHHLSPQALKWREYRRKNPLGVERCKDGPHSFSCSLETKRPGTRVMRRNVFDFPPTNQALSFGRLNGQSLRQLPQCYSDFLPDYFSQTERPPEEFCLSPDAHTDESISIDLLQKRGLVKAINTAVDLIVAHFGTSRDAGVKAKLGNSSVSPNVGHLILKYLCPAIREILNDGLKAYVLDLIIGQRKNQPWSVVEASTQLGPSTRVLHSLFSKVSQYSELTNHSMRLNAFIFGLLNLRSLEFWFNHIYTHEDIIAAHYHPWGFLPLSQGACQPLFEELMLLLQPLSLLPFDLDLLFEPHQLQKGEEHLRRKEQLCSARQSLDQSDRSTFQLMRGCGMLESQETGMLPQRESFVLRDEECRLRTEGVTLKMKQEWQRSVGAEREFRNKEDGVGKECLRKSDTGQMQGGGCCAARMKGVGEDNEKDKKREKDGDATKQRNRQAGWWYQLMQSSQVYIDNSAEGSKFVKWEKRRKGGVENHRQSHPPPREGVVEGAEAIQQMDEQVEHGRTSSSNINSIGRGVLNQSALSEPTKATKAKPSWMGSPPESVLTELKKSKEKQPDCQDPNEGLQAQPEAEEDGTAQVLRWGRLFGAGNASKKEKIEQKSVRKQRLPSGWLSIDHSVLDLVAQSVGVRKRAEQQSFTSQSQESNLGPTEQAQTQSPQTKTLAPREVKALCHHIATEPGQLSFNKGDVLQVLSRADSDWLMCALGDSQGLVPIIYVTLIEDSQER

Target ORF information:

RefSeq Version	XM_005165190.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165190.4

ORF Insert Sequence:

ATGGACAGTC CACCTAAGCT TGCAGGAGAG ACTCTTATTG TCCACCACAT TCCGCTGGTT 
CACTGCCAGG TTTCTAGTTC ACGGAAGTGC TGTGGTAGTT CATTAAAAAG GAACAGTAAT 
CCTTTCAGCT GTCCGGAGAA CCTGGGATTG AGTCGGACGA CCTCCCTACC TGAGAGAGAC 
ATTCTTCAGC GGGAGGCACT GGTGTACAGC AGTCTCATAC AGACCTCAAG CAGCAGCCTC 
AGCTCTAGTG ACACTTTTGG GGATGGGGGG ATGAGAGTAG GAGAGAAACG AGGAAGGGGA 
GGAAGGGAAG GGAGTATGGC AAGTGACACT TCCTCTTTCA CCTCAAGCAA TTCTGAAGAT 
CATGCTCAAA GCCTTTCGAT GAAGACACAT GAACGGCCAA GGCGCAACCC CTTTCTTCTC 
AACACTGAAG ATGAAGATGA TGAGGATGAA GACAATCTAA ATGGATATTT GGAAGACTCC 
TCTTTTCACT TGCATGGAAA TTTGCATGAA ATCTCGAATG CAATGCTTTC AAATGAAGAC 
CTGCCACCTT TCCACCTACA TGACCTTGGG TTCACTGCTG AACCTTTTCT CTTGCATGAG 
TCTACAGAAA AACAGTGGTG TTCAACAAAT GGACCAAGTA GGGAAGCAGA GAGTAGAGAC 
CATAAAAGTG GGACTTTTGA TTTGTCTACT CACATGGAGG GTCTGAACAT GCTGAGATTA 
GATAGCCAGA GACGCCATGG AAGCAGTGGT TCAACCCTTT CTATGGACTG TGGTGAGCAG 
GAATGGGGTG AGGATGATGA TTATGAGGAC CACTCCATGC AGGGGGGAAG AGGCAGCTCA 
GAGTCTTCCA GTCTGGCAGC ACCACACTGC TCATGCTGTG GCCTTTCCCA ACCATACTCT 
GAACCTTTTC AGGAATTTTC TGAGTGTCAC CAGGGCTATG GCAGTGATTC CTCCTGCAAT 
AGCTCAGATG GAGTACTGGT TAACTTCAGC ACGATTTACA ACAAGATGAA CAATGTTGTC 
CCAGCTAAGC AGTCCCTAAA TATCAACAGT TCCACTGAGC AATCCTGTGC CTCATCTGTG 
TCTGAGCTTG TTGGGATGTG CGGGACAGAA TGTAGCAGTG GACGTGGCGC TTTCTACTTA 
GACCTTCACA CCTCCCCAGT CGAACCCCCT CAGCATCCTT TAGCCACACA AGAACATGCA 
AACTCTTCCT CTATATCTCA GCAACAAGGT GCCACTTTGG AAGTAGATGC CAACTGCAAC 
TCCTACCATA ACATTCTTGG AAGTGAGGGA CTGTCGTCTG CTGAAACCTC TGCCAATGAC 
CTCACTTCGT GTCTTCAAAG CCAAGCTCGC TTGGTTGTAG CAACACAGAA TTACTACAAG 
CTTGTGACAT GTGACCTTTC TTCCCAGTCC TCTCCCAGTC CTGTGGGATC ATCGGTCACA 
AGCTGTTCAG ATGAGCACAG CAAAGGGAGT CCCGCTCAAC CAACAGAGTA CTACTTATTT 
CAGCAGATGA AAGAGGATGA AGAAGAGGAA GACACAGAAC TGAATGAAGA AAATGACCCA 
ACTGACATTG ACTCTATTCA TGAGAATGTA ATTGAGGGGC AGGTTTATAT CAACATCTCT 
CCACCTACAA CAGCCTGTAA TTCAGTAGCT GCTTCGGGGA GCAGTCGACC TCGCTCTCGA 
AGCTATGATC GGAACTTGGA CAAGTCTCCA TCTCCTCGTC TTGGATCCTT GGAGCGTATG 
CTCAGCTGCC CTGTTCGGCT CAGTGAGAGT GCAGACCCAA GCCCGCCACC TCCACCACGT 
GTTACTTCCT TTGCTGAAAT TGCAAGGAAT AAGAGAAGAA CCGGAGGATC GCCATCTCAG 
AGGAGTGGGA TCGAGGCCAC GTCCACACAC TCTCATTCCT CTGGAGAGTT CTCGCCCATC 
CTGGAAGGCC TTCCTGAGGG TCAGAGCCAT AGCTTGCCAC CTCTGACTCG ATGTCATAGT 
CAGGGGAGCT GTGATTTGTC CTCTTCATAT AGACCATGGA GTACGCCACT GCGAGAATCT 
CCAGGGGGAA TGGGAAGACA GACTCGATCC AAAGCAGAAG GTGGCCTGTC TAGCTCGACG 
GACTCCTCTC CAACTGTAAT TCGCTATAGC AAAGATCAAC GCCCAACCTC TTTACCTATC 
CAGCCCTTCA CTTTTCAGCA CCAGTTTGGG AAACCCCAGG CAAAGCCTAT CCTTCCTCTT 
CTGGACGGAT ACATAAGCCA AATGCAGAGT CGAGGGGCTG CAGCACCAGA GGGTGATGAA 
GATGACTCTG AAGAACACCA CAGGCCAGCT CACGGCACCT CCAACACACC TACAACAGTG 
CGGCCATCTC CACTGGGAAG CTATTCCCCA ATTCGGCTCC AGGGTGCACC TACCTCTTCT 
GGAAATTGCT CAACATGCTC TCCGACTCTG AATGGTCCAC GGCCACCACG TTCCCTCTCT 
TGCCCCATCT CTGCAGGTCT TCTGCCTCAG CACACCCCAC CTGGAGTGGC CATACATACA 
GAGACTCCTA AACTTGCTCC ATTGCCTCCA ACACCACCTC CAGCTCCCCT CATGAAGCAG 
AGTCCATTGA TGCCTGCGTT GCCCACTAGA AATCACTGCT TCCACCGGGG AAATTTGCCA 
GCGCTTCCCA GCTCTGGTCT AAGTCCTCTT GGACAGTTGG AGCCTGCACC GCAGGAAGAA 
CCAGTAAAGG TTTTACCGGC ATGCGTGACA CAGCGACAGC CTAATGTGCA CCACCTCTCC 
CCACAGGCAC TCAAATGGAG AGAGTACAGG CGCAAGAACC CTCTGGGTGT GGAGCGCTGT 
AAAGATGGCC CTCATTCTTT CTCTTGTTCT TTGGAAACCA AGAGACCTGG GACTCGTGTC 
ATGCGGCGCA ATGTCTTTGA TTTCCCACCA ACCAATCAAG CACTCAGCTT CGGCAGACTG 
AATGGTCAGT CATTGAGGCA GCTGCCGCAG TGCTACAGCG ACTTCCTGCC TGATTATTTC 
TCTCAGACAG AGCGCCCTCC TGAGGAGTTC TGCTTATCTC CTGACGCTCA TACTGATGAA 
TCCATCTCTA TAGACCTGTT GCAGAAAAGA GGTTTGGTGA AAGCCATCAA CACTGCAGTC 
GATCTGATAG TTGCTCATTT TGGCACCAGT CGGGATGCAG GTGTTAAGGC CAAATTGGGT 
AATAGCTCAG TTAGTCCGAA TGTGGGTCAC CTTATTCTGA AGTACTTGTG TCCGGCCATT 
CGGGAGATAC TGAATGATGG ACTGAAAGCA TACGTACTGG ACCTGATCAT TGGACAACGC 
AAAAACCAGC CCTGGAGTGT GGTTGAAGCC TCCACACAAC TTGGACCTTC TACACGAGTT 
TTGCACAGCC TGTTTTCTAA AGTGAGCCAG TATTCTGAGC TGACCAATCA CAGTATGAGA 
CTCAATGCCT TCATCTTTGG CCTGCTAAAC TTGCGATCTC TGGAATTCTG GTTCAATCAC 
ATTTATACGC ATGAAGACAT CATAGCAGCA CATTACCACC CATGGGGTTT CCTACCGCTG 
TCTCAAGGAG CTTGCCAGCC TCTTTTTGAA GAGTTGATGC TCTTGCTTCA GCCACTGTCA 
CTGCTTCCCT TTGACCTTGA CCTGCTATTT GAGCCACACC AGCTTCAGAA AGGAGAAGAA 
CACCTGAGAC GCAAAGAACA GCTGTGCTCT GCTCGCCAGA GCCTCGACCA ATCAGATCGC 
TCCACTTTCC AACTCATGAG AGGCTGTGGA ATGTTGGAGT CTCAGGAAAC GGGAATGCTG 
CCTCAGAGAG AAAGCTTCGT GCTGAGAGAT GAGGAGTGCC GGCTTAGGAC TGAGGGAGTG 
ACATTAAAAA TGAAACAAGA ATGGCAGAGG AGTGTGGGAG CAGAACGTGA GTTTCGGAAT 
AAGGAGGATG GAGTTGGTAA AGAGTGCTTG CGAAAGAGTG ACACAGGACA GATGCAAGGT 
GGAGGGTGCT GTGCTGCCAG AATGAAGGGA GTTGGAGAAG ACAATGAGAA GGACAAAAAG 
AGAGAAAAAG ATGGAGATGC AACCAAACAG AGAAACCGAC AAGCTGGCTG GTGGTATCAG 
TTGATGCAAA GCTCACAGGT TTACATTGAC AACTCTGCCG AGGGGTCGAA ATTTGTCAAG 
TGGGAGAAGA GGAGGAAAGG AGGGGTTGAG AATCACCGTC AAAGCCATCC ACCTCCTAGA 
GAAGGTGTTG TTGAAGGAGC TGAGGCCATT CAGCAGATGG ATGAACAGGT GGAACACGGT 
CGGACCAGTA GTAGCAATAT TAATAGCATT GGCCGTGGGG TTCTAAACCA ATCAGCTTTG 
TCAGAACCCA CCAAAGCCAC AAAAGCGAAG CCATCTTGGA TGGGAAGTCC ACCGGAGTCA 
GTGCTCACTG AGCTGAAAAA GAGTAAGGAG AAGCAGCCAG ATTGCCAGGA TCCGAATGAA 
GGGCTTCAGG CCCAGCCAGA AGCAGAGGAG GATGGAACGG CTCAAGTGCT CCGCTGGGGA 
CGACTATTTG GAGCTGGAAA TGCAAGCAAG AAAGAAAAGA TTGAACAGAA ATCAGTCAGG 
AAACAAAGAT TGCCTTCAGG TTGGTTGAGT ATCGATCATT CTGTGTTGGA TCTGGTGGCT 
CAGTCTGTTG GAGTTCGGAA AAGAGCGGAG CAACAATCTT TTACATCTCA AAGCCAAGAA 
TCCAACCTTG GCCCAACTGA ACAAGCACAA ACACAGAGTC CCCAAACAAA GACACTAGCC 
CCACGGGAAG TGAAGGCGTT ATGCCATCAT ATTGCCACAG AGCCAGGGCA GCTCAGTTTC 
AACAAAGGTG ACGTGCTGCA GGTGCTCAGC AGGGCAGACT CTGATTGGCT GATGTGTGCT 
CTGGGCGATA GCCAGGGCCT TGTTCCCATC ATATACGTCA CCCTGATTGA AGACAGTCAG 
GAGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_005165191.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165248.1 Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

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CloneID	ODa10310
Clone ID Related Accession (Same CDS sequence)	XM_005165191.3 , XM_005165191.4
Accession Version	XM_005165191.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	iporin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTC CACCTAAGCT TGCAGGAGAG ACTCTTATTG TCCACCACAT TCCGCTGGTT 
CACTGCCAGG TTTCTAGTTC ACGGAAGTGC TGTGGTAGTT CATTAAAAAG GAACAGTAAT 
CCTTTCAGCT GTCCGGAGAA CCTGGGATTG AGTCGGACGA CCTCCCTACC TGAGAGAGAC 
ATTCTTCAGC GGGAGGCACT GGTGTACAGC AGTCTCATAC AGACCTCAAG CAGCAGCCTC 
AGCTCTAGTG ACACTTTTGG GGATGGGGGG ATGAGAGTAG GAGAGAAACG AGGAAGGGGA 
GGAAGGGAAG GGAGTATGGC AAGTGACACT TCCTCTTTCA CCTCAAGCAA TTCTGAAGAT 
CATGCTCAAA GCCTTTCGAT GAAGACACAT GAACGGCCAA GGCGCAACCC CTTTCTTCTC 
AACACTGAAG ATGAAGATGA TGAGGATGAA GACAATCTAA ATGGATATTT GGAAGACTCC 
TCTTTTCACT TGCATGGAAA TTTGCATGAA ATCTCGAATG CAATGCTTTC AAATGAAGAC 
CTGCCACCTT TCCACCTACA TGACCTTGGG TTCACTGCTG AACCTTTTCT CTTGCATGAG 
TCTACAGAAA AACAGTGGTG TTCAACAAAT GGACCAAGTA GGGAAGCAGA GAGTAGAGAC 
CATAAAAGTG GGACTTTTGA TTTGTCTACT CACATGGAGG GTCTGAACAT GCTGAGATTA 
GATAGCCAGA GACGCCATGG AAGCAGTGGT TCAACCCTTT CTATGGACTG TGGTGAGCAG 
GAATGGGGTG AGGATGATGA TTATGAGGAC CACTCCATGC AGGGGGGAAG AGGCAGCTCA 
GAGTCTTCCA GTCTGGCAGC ACCACACTGC TCATGCTGTG GCCTTTCCCA ACCATACTCT 
GAACCTTTTC AGGAATTTTC TGAGTGTCAC CAGGGCTATG GCAGTGATTC CTCCTGCAAT 
AGCTCAGATG GAGTACTGGT TAACTTCAGC ACGATTTACA ACAAGATGAA CAATGTTGTC 
CCAGCTAAGC AGTCCCTAAA TATCAACAGT TCCACTGAGC AATCCTGTGC CTCATCTGTG 
TCTGAGCTTG TTGGGATGTG CGGGACAGAA TGTAGCAGTG GACGTGGCGC TTTCTACTTA 
GACCTTCACA CCTCCCCAGT CGAACCCCCT CAGCATCCTT TAGCCACACA AGAACATGCA 
AACTCTTCCT CTATATCTCA GCAACAAGGT GCCACTTTGG AAGTAGATGC CAACTGCAAC 
TCCTACCATA ACATTCTTGG AAGTGAGGGA CTGTCGTCTG CTGAAACCTC TGCCAATGAC 
CTCACTTCGT GTCTTCAAAG CCAAGCTCGC TTGGTTGTAG CAACACAGAA TTACTACAAG 
CTTGTGACAT GTGACCTTTC TTCCCAGTCC TCTCCCAGTC CTGTGGGATC ATCGGTCACA 
AGCTGTTCAG ATGAGCACAG CAAAGGGAGT CCCGCTCAAC CAACAGAGTA CTACTTATTT 
CAGCAGATGA AAGAGGATGA AGAAGAGGAA GACACAGAAC TGAATGAAGA AAATGACCCA 
ACTGACATTG ACTCTATTCA TGAGAATGTA ATTGAGGGGC AGGTTTATAT CAACATCTCT 
CCACCTACAA CAGCCTGTAA TTCAGTAGCT GCTTCGGGGA GCAGTCGACC TCGCTCTCGA 
AGCTATGATC GGAACTTGGA CAAGTCTCCA TCTCCTCGTC TTGGATCCTT GGAGCGTATG 
CTCAGCTGCC CTGTTCGGCT CAGTGAGAGT GCAGACCCAA GCCCGCCACC TCCACCACGT 
GTTACTTCCT TTGCTGAAAT TGCAAGGAAT AAGAGAAGAA CCGGAGGATC GCCATCTCAG 
AGGAGTGGGA TCGAGGCCAC GTCCACACAC TCTCATTCCT CTGGAGAGTT CTCGCCCATC 
CTGGAAGGCC TTCCTGAGGG TCAGAGCCAT AGCTTGCCAC CTCTGACTCG ATGTCATAGT 
CAGGGGAGCT GTGATTTGTC CTCTTCATAT AGACCATGGA GTACGCCACT GCGAGAATCT 
CCAGGGGGAA TGGGAAGACA GACTCGATCC AAAGCAGAAG GTGGCCTGTC TAGCTCGACG 
GACTCCTCTC CAACTGTAAT TCGCTATAGC AAAGATCAAC GCCCAACCTC TTTACCTATC 
CAGCCCTTCA CTTTTCAGCA CCAGTTTGGG AAACCCCAGG CAAAGCCTAT CCTTCCTCTT 
CTGGACGGAT ACATAAGCCA AATGCAGAGT CGAGGGGCTG CAGCACCAGA GGGTGATGAA 
GATGACTCTG AAGAACACCA CAGGCCAGCT CACGGCACCT CCAACACACC TACAACAGTG 
CGGCCATCTC CACTGGGAAG CTATTCCCCA ATTCGGCTCC AGGGTGCACC TACCTCTTCT 
GGAAATTGCT CAACATGCTC TCCGACTCTG AATGGTCCAC GGCCACCACG TTCCCTCTCT 
TGCCCCATCT CTGCAGGTCT TCTGCCTCAG CACACCCCAC CTGGAGTGGC CATACATACA 
GAGACTCCTA AACTTGCTCC ATTGCCTCCA ACACCACCTC CAGCTCCCCT CATGAAGCAG 
AGTCCATTGA TGCCTGCGTT GCCCACTAGA AATCACTGCT TCCACCGGGG AAATTTGCCA 
GCGCTTCCCA GCTCTGGTCT AAGTCCTCTT GGACAGTTGG AGCCTGCACC GCAGGAAGAA 
CCAGTAAAGG TTTTACCGGC ATGCGTGACA CAGCGACAGC CTAATGGTCA GTCATTGAGG 
CAGCTGCCGC AGTGCTACAG CGACTTCCTG CCTGATTATT TCTCTCAGAC AGAGCGCCCT 
CCTGAGGAGT TCTGCTTATC TCCTGACGCT CATACTGATG AATCCATCTC TATAGACCTG 
TTGCAGAAAA GAGGTTTGGT GAAAGCCATC AACACTGCAG TCGATCTGAT AGTTGCTCAT 
TTTGGCACCA GTCGGGATGC AGGTGTTAAG GCCAAATTGG GTAATAGCTC AGTTAGTCCG 
AATGTGGGTC ACCTTATTCT GAAGTACTTG TGTCCGGCCA TTCGGGAGAT ACTGAATGAT 
GGACTGAAAG CATACGTACT GGACCTGATC ATTGGACAAC GCAAAAACCA GCCCTGGAGT 
GTGGTTGAAG CCTCCACACA ACTTGGACCT TCTACACGAG TTTTGCACAG CCTGTTTTCT 
AAAGTGAGCC AGTATTCTGA GCTGACCAAT CACAGTATGA GACTCAATGC CTTCATCTTT 
GGCCTGCTAA ACTTGCGATC TCTGGAATTC TGGTTCAATC ACATTTATAC GCATGAAGAC 
ATCATAGCAG CACATTACCA CCCATGGGGT TTCCTACCGC TGTCTCAAGG AGCTTGCCAG 
CCTCTTTTTG AAGAGTTGAT GCTCTTGCTT CAGCCACTGT CACTGCTTCC CTTTGACCTT 
GACCTGCTAT TTGAGCCACA CCAGCTTCAG AAAGGAGAAG AACACCTGAG ACGCAAAGAA 
CAGCTGTGCT CTGCTCGCCA GAGCCTCGAC CAATCAGATC GCTCCACTTT CCAACTCATG 
AGAGGCTGTG GAATGTTGGA GTCTCAGGAA ACGGGAATGC TGCCTCAGAG AGAAAGCTTC 
GTGCTGAGAG ATGAGGAGTG CCGGCTTAGG ACTGAGGGAG TGACATTAAA AATGAAACAA 
GAATGGCAGA GGAGTGTGGG AGCAGAACGT GAGTTTCGGA ATAAGGAGGA TGGAGTTGGT 
AAAGAGTGCT TGCGAAAGAG TGACACAGGA CAGATGCAAG GTGGAGGGTG CTGTGCTGCC 
AGAATGAAGG GAGTTGGAGA AGACAATGAG AAGGACAAAA AGAGAGAAAA AGATGGAGAT 
GCAACCAAAC AGAGAAACCG ACAAGCTGGC TGGTGGTATC AGTTGATGCA AAGCTCACAG 
GTTTACATTG ACAACTCTGC CGAGGGGTCG AAATTTGTCA AGTGGGAGAA GAGGAGGAAA 
GGAGGGGTTG AGAATCACCG TCAAAGCCAT CCACCTCCTA GAGAAGGTGT TGTTGAAGGA 
GCTGAGGCCA TTCAGCAGAT GGATGAACAG GTGGAACACG GTCGGACCAG TAGTAGCAAT 
ATTAATAGCA TTGGCCGTGG GGTTCTAAAC CAATCAGCTT TGTCAGAACC CACCAAAGCC 
ACAAAAGCGA AGCCATCTTG GATGGGAAGT CCACCGGAGT CAGTGCTCAC TGAGCTGAAA 
AAGAGTAAGG AGAAGCAGCC AGATTGCCAG GATCCGAATG AAGGGCTTCA GGCCCAGCCA 
GAAGCAGAGG AGGATGGAAC GGCTCAAGTG CTCCGCTGGG GACGACTATT TGGAGCTGGA 
AATGCAAGCA AGAAAGAAAA GATTGAACAG AAATCAGTCA GGAAACAAAG ATTGCCTTCA 
GGTTGGTTGA GTATCGATCA TTCTGTGTTG GATCTGGTGG CTCAGTCTGT TGGAGTTCGG 
AAAAGAGCGG AGCAACAATC TTTTACATCT CAAAGCCAAG AATCCAACCT TGGCCCAACT 
GAACAAGCAC AAACACAGAG TCCCCAAACA AAGACACTAG CCCCACGGGA AGTGAAGGCG 
TTATGCCATC ATATTGCCAC AGAGCCAGGG CAGCTCAGTT TCAACAAAGG TGACGTGCTG 
CAGGTGCTCA GCAGGGCAGA CTCTGATTGG CTGATGTGTG CTCTGGGCGA TAGCCAGGGC 
CTTGTTCCCA TCATATACGT CACCCTGATT GAAGACAGTC AGGAGCGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165248.1
CDS	494..5224
Translation	MDSPPKLAGETLIVHHIPLVHCQVSSSRKCCGSSLKRNSNPFSCPENLGLSRTTSLPERDILQREALVYSSLIQTSSSSLSSSDTFGDGGMRVGEKRGRGGREGSMASDTSSFTSSNSEDHAQSLSMKTHERPRRNPFLLNTEDEDDEDEDNLNGYLEDSSFHLHGNLHEISNAMLSNEDLPPFHLHDLGFTAEPFLLHESTEKQWCSTNGPSREAESRDHKSGTFDLSTHMEGLNMLRLDSQRRHGSSGSTLSMDCGEQEWGEDDDYEDHSMQGGRGSSESSSLAAPHCSCCGLSQPYSEPFQEFSECHQGYGSDSSCNSSDGVLVNFSTIYNKMNNVVPAKQSLNINSSTEQSCASSVSELVGMCGTECSSGRGAFYLDLHTSPVEPPQHPLATQEHANSSSISQQQGATLEVDANCNSYHNILGSEGLSSAETSANDLTSCLQSQARLVVATQNYYKLVTCDLSSQSSPSPVGSSVTSCSDEHSKGSPAQPTEYYLFQQMKEDEEEEDTELNEENDPTDIDSIHENVIEGQVYINISPPTTACNSVAASGSSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSESADPSPPPPPRVTSFAEIARNKRRTGGSPSQRSGIEATSTHSHSSGEFSPILEGLPEGQSHSLPPLTRCHSQGSCDLSSSYRPWSTPLRESPGGMGRQTRSKAEGGLSSSTDSSPTVIRYSKDQRPTSLPIQPFTFQHQFGKPQAKPILPLLDGYISQMQSRGAAAPEGDEDDSEEHHRPAHGTSNTPTTVRPSPLGSYSPIRLQGAPTSSGNCSTCSPTLNGPRPPRSLSCPISAGLLPQHTPPGVAIHTETPKLAPLPPTPPPAPLMKQSPLMPALPTRNHCFHRGNLPALPSSGLSPLGQLEPAPQEEPVKVLPACVTQRQPNGQSLRQLPQCYSDFLPDYFSQTERPPEEFCLSPDAHTDESISIDLLQKRGLVKAINTAVDLIVAHFGTSRDAGVKAKLGNSSVSPNVGHLILKYLCPAIREILNDGLKAYVLDLIIGQRKNQPWSVVEASTQLGPSTRVLHSLFSKVSQYSELTNHSMRLNAFIFGLLNLRSLEFWFNHIYTHEDIIAAHYHPWGFLPLSQGACQPLFEELMLLLQPLSLLPFDLDLLFEPHQLQKGEEHLRRKEQLCSARQSLDQSDRSTFQLMRGCGMLESQETGMLPQRESFVLRDEECRLRTEGVTLKMKQEWQRSVGAEREFRNKEDGVGKECLRKSDTGQMQGGGCCAARMKGVGEDNEKDKKREKDGDATKQRNRQAGWWYQLMQSSQVYIDNSAEGSKFVKWEKRRKGGVENHRQSHPPPREGVVEGAEAIQQMDEQVEHGRTSSSNINSIGRGVLNQSALSEPTKATKAKPSWMGSPPESVLTELKKSKEKQPDCQDPNEGLQAQPEAEEDGTAQVLRWGRLFGAGNASKKEKIEQKSVRKQRLPSGWLSIDHSVLDLVAQSVGVRKRAEQQSFTSQSQESNLGPTEQAQTQSPQTKTLAPREVKALCHHIATEPGQLSFNKGDVLQVLSRADSDWLMCALGDSQGLVPIIYVTLIEDSQER

Target ORF information:

RefSeq Version	XM_005165191.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165191.3

ORF Insert Sequence:

ATGGACAGTC CACCTAAGCT TGCAGGAGAG ACTCTTATTG TCCACCACAT TCCGCTGGTT 
CACTGCCAGG TTTCTAGTTC ACGGAAGTGC TGTGGTAGTT CATTAAAAAG GAACAGTAAT 
CCTTTCAGCT GTCCGGAGAA CCTGGGATTG AGTCGGACGA CCTCCCTACC TGAGAGAGAC 
ATTCTTCAGC GGGAGGCACT GGTGTACAGC AGTCTCATAC AGACCTCAAG CAGCAGCCTC 
AGCTCTAGTG ACACTTTTGG GGATGGGGGG ATGAGAGTAG GAGAGAAACG AGGAAGGGGA 
GGAAGGGAAG GGAGTATGGC AAGTGACACT TCCTCTTTCA CCTCAAGCAA TTCTGAAGAT 
CATGCTCAAA GCCTTTCGAT GAAGACACAT GAACGGCCAA GGCGCAACCC CTTTCTTCTC 
AACACTGAAG ATGAAGATGA TGAGGATGAA GACAATCTAA ATGGATATTT GGAAGACTCC 
TCTTTTCACT TGCATGGAAA TTTGCATGAA ATCTCGAATG CAATGCTTTC AAATGAAGAC 
CTGCCACCTT TCCACCTACA TGACCTTGGG TTCACTGCTG AACCTTTTCT CTTGCATGAG 
TCTACAGAAA AACAGTGGTG TTCAACAAAT GGACCAAGTA GGGAAGCAGA GAGTAGAGAC 
CATAAAAGTG GGACTTTTGA TTTGTCTACT CACATGGAGG GTCTGAACAT GCTGAGATTA 
GATAGCCAGA GACGCCATGG AAGCAGTGGT TCAACCCTTT CTATGGACTG TGGTGAGCAG 
GAATGGGGTG AGGATGATGA TTATGAGGAC CACTCCATGC AGGGGGGAAG AGGCAGCTCA 
GAGTCTTCCA GTCTGGCAGC ACCACACTGC TCATGCTGTG GCCTTTCCCA ACCATACTCT 
GAACCTTTTC AGGAATTTTC TGAGTGTCAC CAGGGCTATG GCAGTGATTC CTCCTGCAAT 
AGCTCAGATG GAGTACTGGT TAACTTCAGC ACGATTTACA ACAAGATGAA CAATGTTGTC 
CCAGCTAAGC AGTCCCTAAA TATCAACAGT TCCACTGAGC AATCCTGTGC CTCATCTGTG 
TCTGAGCTTG TTGGGATGTG CGGGACAGAA TGTAGCAGTG GACGTGGCGC TTTCTACTTA 
GACCTTCACA CCTCCCCAGT CGAACCCCCT CAGCATCCTT TAGCCACACA AGAACATGCA 
AACTCTTCCT CTATATCTCA GCAACAAGGT GCCACTTTGG AAGTAGATGC CAACTGCAAC 
TCCTACCATA ACATTCTTGG AAGTGAGGGA CTGTCGTCTG CTGAAACCTC TGCCAATGAC 
CTCACTTCGT GTCTTCAAAG CCAAGCTCGC TTGGTTGTAG CAACACAGAA TTACTACAAG 
CTTGTGACAT GTGACCTTTC TTCCCAGTCC TCTCCCAGTC CTGTGGGATC ATCGGTCACA 
AGCTGTTCAG ATGAGCACAG CAAAGGGAGT CCCGCTCAAC CAACAGAGTA CTACTTATTT 
CAGCAGATGA AAGAGGATGA AGAAGAGGAA GACACAGAAC TGAATGAAGA AAATGACCCA 
ACTGACATTG ACTCTATTCA TGAGAATGTA ATTGAGGGGC AGGTTTATAT CAACATCTCT 
CCACCTACAA CAGCCTGTAA TTCAGTAGCT GCTTCGGGGA GCAGTCGACC TCGCTCTCGA 
AGCTATGATC GGAACTTGGA CAAGTCTCCA TCTCCTCGTC TTGGATCCTT GGAGCGTATG 
CTCAGCTGCC CTGTTCGGCT CAGTGAGAGT GCAGACCCAA GCCCGCCACC TCCACCACGT 
GTTACTTCCT TTGCTGAAAT TGCAAGGAAT AAGAGAAGAA CCGGAGGATC GCCATCTCAG 
AGGAGTGGGA TCGAGGCCAC GTCCACACAC TCTCATTCCT CTGGAGAGTT CTCGCCCATC 
CTGGAAGGCC TTCCTGAGGG TCAGAGCCAT AGCTTGCCAC CTCTGACTCG ATGTCATAGT 
CAGGGGAGCT GTGATTTGTC CTCTTCATAT AGACCATGGA GTACGCCACT GCGAGAATCT 
CCAGGGGGAA TGGGAAGACA GACTCGATCC AAAGCAGAAG GTGGCCTGTC TAGCTCGACG 
GACTCCTCTC CAACTGTAAT TCGCTATAGC AAAGATCAAC GCCCAACCTC TTTACCTATC 
CAGCCCTTCA CTTTTCAGCA CCAGTTTGGG AAACCCCAGG CAAAGCCTAT CCTTCCTCTT 
CTGGACGGAT ACATAAGCCA AATGCAGAGT CGAGGGGCTG CAGCACCAGA GGGTGATGAA 
GATGACTCTG AAGAACACCA CAGGCCAGCT CACGGCACCT CCAACACACC TACAACAGTG 
CGGCCATCTC CACTGGGAAG CTATTCCCCA ATTCGGCTCC AGGGTGCACC TACCTCTTCT 
GGAAATTGCT CAACATGCTC TCCGACTCTG AATGGTCCAC GGCCACCACG TTCCCTCTCT 
TGCCCCATCT CTGCAGGTCT TCTGCCTCAG CACACCCCAC CTGGAGTGGC CATACATACA 
GAGACTCCTA AACTTGCTCC ATTGCCTCCA ACACCACCTC CAGCTCCCCT CATGAAGCAG 
AGTCCATTGA TGCCTGCGTT GCCCACTAGA AATCACTGCT TCCACCGGGG AAATTTGCCA 
GCGCTTCCCA GCTCTGGTCT AAGTCCTCTT GGACAGTTGG AGCCTGCACC GCAGGAAGAA 
CCAGTAAAGG TTTTACCGGC ATGCGTGACA CAGCGACAGC CTAATGGTCA GTCATTGAGG 
CAGCTGCCGC AGTGCTACAG CGACTTCCTG CCTGATTATT TCTCTCAGAC AGAGCGCCCT 
CCTGAGGAGT TCTGCTTATC TCCTGACGCT CATACTGATG AATCCATCTC TATAGACCTG 
TTGCAGAAAA GAGGTTTGGT GAAAGCCATC AACACTGCAG TCGATCTGAT AGTTGCTCAT 
TTTGGCACCA GTCGGGATGC AGGTGTTAAG GCCAAATTGG GTAATAGCTC AGTTAGTCCG 
AATGTGGGTC ACCTTATTCT GAAGTACTTG TGTCCGGCCA TTCGGGAGAT ACTGAATGAT 
GGACTGAAAG CATACGTACT GGACCTGATC ATTGGACAAC GCAAAAACCA GCCCTGGAGT 
GTGGTTGAAG CCTCCACACA ACTTGGACCT TCTACACGAG TTTTGCACAG CCTGTTTTCT 
AAAGTGAGCC AGTATTCTGA GCTGACCAAT CACAGTATGA GACTCAATGC CTTCATCTTT 
GGCCTGCTAA ACTTGCGATC TCTGGAATTC TGGTTCAATC ACATTTATAC GCATGAAGAC 
ATCATAGCAG CACATTACCA CCCATGGGGT TTCCTACCGC TGTCTCAAGG AGCTTGCCAG 
CCTCTTTTTG AAGAGTTGAT GCTCTTGCTT CAGCCACTGT CACTGCTTCC CTTTGACCTT 
GACCTGCTAT TTGAGCCACA CCAGCTTCAG AAAGGAGAAG AACACCTGAG ACGCAAAGAA 
CAGCTGTGCT CTGCTCGCCA GAGCCTCGAC CAATCAGATC GCTCCACTTT CCAACTCATG 
AGAGGCTGTG GAATGTTGGA GTCTCAGGAA ACGGGAATGC TGCCTCAGAG AGAAAGCTTC 
GTGCTGAGAG ATGAGGAGTG CCGGCTTAGG ACTGAGGGAG TGACATTAAA AATGAAACAA 
GAATGGCAGA GGAGTGTGGG AGCAGAACGT GAGTTTCGGA ATAAGGAGGA TGGAGTTGGT 
AAAGAGTGCT TGCGAAAGAG TGACACAGGA CAGATGCAAG GTGGAGGGTG CTGTGCTGCC 
AGAATGAAGG GAGTTGGAGA AGACAATGAG AAGGACAAAA AGAGAGAAAA AGATGGAGAT 
GCAACCAAAC AGAGAAACCG ACAAGCTGGC TGGTGGTATC AGTTGATGCA AAGCTCACAG 
GTTTACATTG ACAACTCTGC CGAGGGGTCG AAATTTGTCA AGTGGGAGAA GAGGAGGAAA 
GGAGGGGTTG AGAATCACCG TCAAAGCCAT CCACCTCCTA GAGAAGGTGT TGTTGAAGGA 
GCTGAGGCCA TTCAGCAGAT GGATGAACAG GTGGAACACG GTCGGACCAG TAGTAGCAAT 
ATTAATAGCA TTGGCCGTGG GGTTCTAAAC CAATCAGCTT TGTCAGAACC CACCAAAGCC 
ACAAAAGCGA AGCCATCTTG GATGGGAAGT CCACCGGAGT CAGTGCTCAC TGAGCTGAAA 
AAGAGTAAGG AGAAGCAGCC AGATTGCCAG GATCCGAATG AAGGGCTTCA GGCCCAGCCA 
GAAGCAGAGG AGGATGGAAC GGCTCAAGTG CTCCGCTGGG GACGACTATT TGGAGCTGGA 
AATGCAAGCA AGAAAGAAAA GATTGAACAG AAATCAGTCA GGAAACAAAG ATTGCCTTCA 
GGTTGGTTGA GTATCGATCA TTCTGTGTTG GATCTGGTGG CTCAGTCTGT TGGAGTTCGG 
AAAAGAGCGG AGCAACAATC TTTTACATCT CAAAGCCAAG AATCCAACCT TGGCCCAACT 
GAACAAGCAC AAACACAGAG TCCCCAAACA AAGACACTAG CCCCACGGGA AGTGAAGGCG 
TTATGCCATC ATATTGCCAC AGAGCCAGGG CAGCTCAGTT TCAACAAAGG TGACGTGCTG 
CAGGTGCTCA GCAGGGCAGA CTCTGATTGG CTGATGTGTG CTCTGGGCGA TAGCCAGGGC 
CTTGTTCCCA TCATATACGT CACCCTGATT GAAGACAGTC AGGAGCGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_005165191.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165248.1 Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

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CloneID	ODa10310
Clone ID Related Accession (Same CDS sequence)	XM_005165191.3 , XM_005165191.4
Accession Version	XM_005165191.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	iporin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165191.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTC CACCTAAGCT TGCAGGAGAG ACTCTTATTG TCCACCACAT TCCGCTGGTT 
CACTGCCAGG TTTCTAGTTC ACGGAAGTGC TGTGGTAGTT CATTAAAAAG GAACAGTAAT 
CCTTTCAGCT GTCCGGAGAA CCTGGGATTG AGTCGGACGA CCTCCCTACC TGAGAGAGAC 
ATTCTTCAGC GGGAGGCACT GGTGTACAGC AGTCTCATAC AGACCTCAAG CAGCAGCCTC 
AGCTCTAGTG ACACTTTTGG GGATGGGGGG ATGAGAGTAG GAGAGAAACG AGGAAGGGGA 
GGAAGGGAAG GGAGTATGGC AAGTGACACT TCCTCTTTCA CCTCAAGCAA TTCTGAAGAT 
CATGCTCAAA GCCTTTCGAT GAAGACACAT GAACGGCCAA GGCGCAACCC CTTTCTTCTC 
AACACTGAAG ATGAAGATGA TGAGGATGAA GACAATCTAA ATGGATATTT GGAAGACTCC 
TCTTTTCACT TGCATGGAAA TTTGCATGAA ATCTCGAATG CAATGCTTTC AAATGAAGAC 
CTGCCACCTT TCCACCTACA TGACCTTGGG TTCACTGCTG AACCTTTTCT CTTGCATGAG 
TCTACAGAAA AACAGTGGTG TTCAACAAAT GGACCAAGTA GGGAAGCAGA GAGTAGAGAC 
CATAAAAGTG GGACTTTTGA TTTGTCTACT CACATGGAGG GTCTGAACAT GCTGAGATTA 
GATAGCCAGA GACGCCATGG AAGCAGTGGT TCAACCCTTT CTATGGACTG TGGTGAGCAG 
GAATGGGGTG AGGATGATGA TTATGAGGAC CACTCCATGC AGGGGGGAAG AGGCAGCTCA 
GAGTCTTCCA GTCTGGCAGC ACCACACTGC TCATGCTGTG GCCTTTCCCA ACCATACTCT 
GAACCTTTTC AGGAATTTTC TGAGTGTCAC CAGGGCTATG GCAGTGATTC CTCCTGCAAT 
AGCTCAGATG GAGTACTGGT TAACTTCAGC ACGATTTACA ACAAGATGAA CAATGTTGTC 
CCAGCTAAGC AGTCCCTAAA TATCAACAGT TCCACTGAGC AATCCTGTGC CTCATCTGTG 
TCTGAGCTTG TTGGGATGTG CGGGACAGAA TGTAGCAGTG GACGTGGCGC TTTCTACTTA 
GACCTTCACA CCTCCCCAGT CGAACCCCCT CAGCATCCTT TAGCCACACA AGAACATGCA 
AACTCTTCCT CTATATCTCA GCAACAAGGT GCCACTTTGG AAGTAGATGC CAACTGCAAC 
TCCTACCATA ACATTCTTGG AAGTGAGGGA CTGTCGTCTG CTGAAACCTC TGCCAATGAC 
CTCACTTCGT GTCTTCAAAG CCAAGCTCGC TTGGTTGTAG CAACACAGAA TTACTACAAG 
CTTGTGACAT GTGACCTTTC TTCCCAGTCC TCTCCCAGTC CTGTGGGATC ATCGGTCACA 
AGCTGTTCAG ATGAGCACAG CAAAGGGAGT CCCGCTCAAC CAACAGAGTA CTACTTATTT 
CAGCAGATGA AAGAGGATGA AGAAGAGGAA GACACAGAAC TGAATGAAGA AAATGACCCA 
ACTGACATTG ACTCTATTCA TGAGAATGTA ATTGAGGGGC AGGTTTATAT CAACATCTCT 
CCACCTACAA CAGCCTGTAA TTCAGTAGCT GCTTCGGGGA GCAGTCGACC TCGCTCTCGA 
AGCTATGATC GGAACTTGGA CAAGTCTCCA TCTCCTCGTC TTGGATCCTT GGAGCGTATG 
CTCAGCTGCC CTGTTCGGCT CAGTGAGAGT GCAGACCCAA GCCCGCCACC TCCACCACGT 
GTTACTTCCT TTGCTGAAAT TGCAAGGAAT AAGAGAAGAA CCGGAGGATC GCCATCTCAG 
AGGAGTGGGA TCGAGGCCAC GTCCACACAC TCTCATTCCT CTGGAGAGTT CTCGCCCATC 
CTGGAAGGCC TTCCTGAGGG TCAGAGCCAT AGCTTGCCAC CTCTGACTCG ATGTCATAGT 
CAGGGGAGCT GTGATTTGTC CTCTTCATAT AGACCATGGA GTACGCCACT GCGAGAATCT 
CCAGGGGGAA TGGGAAGACA GACTCGATCC AAAGCAGAAG GTGGCCTGTC TAGCTCGACG 
GACTCCTCTC CAACTGTAAT TCGCTATAGC AAAGATCAAC GCCCAACCTC TTTACCTATC 
CAGCCCTTCA CTTTTCAGCA CCAGTTTGGG AAACCCCAGG CAAAGCCTAT CCTTCCTCTT 
CTGGACGGAT ACATAAGCCA AATGCAGAGT CGAGGGGCTG CAGCACCAGA GGGTGATGAA 
GATGACTCTG AAGAACACCA CAGGCCAGCT CACGGCACCT CCAACACACC TACAACAGTG 
CGGCCATCTC CACTGGGAAG CTATTCCCCA ATTCGGCTCC AGGGTGCACC TACCTCTTCT 
GGAAATTGCT CAACATGCTC TCCGACTCTG AATGGTCCAC GGCCACCACG TTCCCTCTCT 
TGCCCCATCT CTGCAGGTCT TCTGCCTCAG CACACCCCAC CTGGAGTGGC CATACATACA 
GAGACTCCTA AACTTGCTCC ATTGCCTCCA ACACCACCTC CAGCTCCCCT CATGAAGCAG 
AGTCCATTGA TGCCTGCGTT GCCCACTAGA AATCACTGCT TCCACCGGGG AAATTTGCCA 
GCGCTTCCCA GCTCTGGTCT AAGTCCTCTT GGACAGTTGG AGCCTGCACC GCAGGAAGAA 
CCAGTAAAGG TTTTACCGGC ATGCGTGACA CAGCGACAGC CTAATGGTCA GTCATTGAGG 
CAGCTGCCGC AGTGCTACAG CGACTTCCTG CCTGATTATT TCTCTCAGAC AGAGCGCCCT 
CCTGAGGAGT TCTGCTTATC TCCTGACGCT CATACTGATG AATCCATCTC TATAGACCTG 
TTGCAGAAAA GAGGTTTGGT GAAAGCCATC AACACTGCAG TCGATCTGAT AGTTGCTCAT 
TTTGGCACCA GTCGGGATGC AGGTGTTAAG GCCAAATTGG GTAATAGCTC AGTTAGTCCG 
AATGTGGGTC ACCTTATTCT GAAGTACTTG TGTCCGGCCA TTCGGGAGAT ACTGAATGAT 
GGACTGAAAG CATACGTACT GGACCTGATC ATTGGACAAC GCAAAAACCA GCCCTGGAGT 
GTGGTTGAAG CCTCCACACA ACTTGGACCT TCTACACGAG TTTTGCACAG CCTGTTTTCT 
AAAGTGAGCC AGTATTCTGA GCTGACCAAT CACAGTATGA GACTCAATGC CTTCATCTTT 
GGCCTGCTAA ACTTGCGATC TCTGGAATTC TGGTTCAATC ACATTTATAC GCATGAAGAC 
ATCATAGCAG CACATTACCA CCCATGGGGT TTCCTACCGC TGTCTCAAGG AGCTTGCCAG 
CCTCTTTTTG AAGAGTTGAT GCTCTTGCTT CAGCCACTGT CACTGCTTCC CTTTGACCTT 
GACCTGCTAT TTGAGCCACA CCAGCTTCAG AAAGGAGAAG AACACCTGAG ACGCAAAGAA 
CAGCTGTGCT CTGCTCGCCA GAGCCTCGAC CAATCAGATC GCTCCACTTT CCAACTCATG 
AGAGGCTGTG GAATGTTGGA GTCTCAGGAA ACGGGAATGC TGCCTCAGAG AGAAAGCTTC 
GTGCTGAGAG ATGAGGAGTG CCGGCTTAGG ACTGAGGGAG TGACATTAAA AATGAAACAA 
GAATGGCAGA GGAGTGTGGG AGCAGAACGT GAGTTTCGGA ATAAGGAGGA TGGAGTTGGT 
AAAGAGTGCT TGCGAAAGAG TGACACAGGA CAGATGCAAG GTGGAGGGTG CTGTGCTGCC 
AGAATGAAGG GAGTTGGAGA AGACAATGAG AAGGACAAAA AGAGAGAAAA AGATGGAGAT 
GCAACCAAAC AGAGAAACCG ACAAGCTGGC TGGTGGTATC AGTTGATGCA AAGCTCACAG 
GTTTACATTG ACAACTCTGC CGAGGGGTCG AAATTTGTCA AGTGGGAGAA GAGGAGGAAA 
GGAGGGGTTG AGAATCACCG TCAAAGCCAT CCACCTCCTA GAGAAGGTGT TGTTGAAGGA 
GCTGAGGCCA TTCAGCAGAT GGATGAACAG GTGGAACACG GTCGGACCAG TAGTAGCAAT 
ATTAATAGCA TTGGCCGTGG GGTTCTAAAC CAATCAGCTT TGTCAGAACC CACCAAAGCC 
ACAAAAGCGA AGCCATCTTG GATGGGAAGT CCACCGGAGT CAGTGCTCAC TGAGCTGAAA 
AAGAGTAAGG AGAAGCAGCC AGATTGCCAG GATCCGAATG AAGGGCTTCA GGCCCAGCCA 
GAAGCAGAGG AGGATGGAAC GGCTCAAGTG CTCCGCTGGG GACGACTATT TGGAGCTGGA 
AATGCAAGCA AGAAAGAAAA GATTGAACAG AAATCAGTCA GGAAACAAAG ATTGCCTTCA 
GGTTGGTTGA GTATCGATCA TTCTGTGTTG GATCTGGTGG CTCAGTCTGT TGGAGTTCGG 
AAAAGAGCGG AGCAACAATC TTTTACATCT CAAAGCCAAG AATCCAACCT TGGCCCAACT 
GAACAAGCAC AAACACAGAG TCCCCAAACA AAGACACTAG CCCCACGGGA AGTGAAGGCG 
TTATGCCATC ATATTGCCAC AGAGCCAGGG CAGCTCAGTT TCAACAAAGG TGACGTGCTG 
CAGGTGCTCA GCAGGGCAGA CTCTGATTGG CTGATGTGTG CTCTGGGCGA TAGCCAGGGC 
CTTGTTCCCA TCATATACGT CACCCTGATT GAAGACAGTC AGGAGCGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165248.1
CDS	452..5182
Translation	MDSPPKLAGETLIVHHIPLVHCQVSSSRKCCGSSLKRNSNPFSCPENLGLSRTTSLPERDILQREALVYSSLIQTSSSSLSSSDTFGDGGMRVGEKRGRGGREGSMASDTSSFTSSNSEDHAQSLSMKTHERPRRNPFLLNTEDEDDEDEDNLNGYLEDSSFHLHGNLHEISNAMLSNEDLPPFHLHDLGFTAEPFLLHESTEKQWCSTNGPSREAESRDHKSGTFDLSTHMEGLNMLRLDSQRRHGSSGSTLSMDCGEQEWGEDDDYEDHSMQGGRGSSESSSLAAPHCSCCGLSQPYSEPFQEFSECHQGYGSDSSCNSSDGVLVNFSTIYNKMNNVVPAKQSLNINSSTEQSCASSVSELVGMCGTECSSGRGAFYLDLHTSPVEPPQHPLATQEHANSSSISQQQGATLEVDANCNSYHNILGSEGLSSAETSANDLTSCLQSQARLVVATQNYYKLVTCDLSSQSSPSPVGSSVTSCSDEHSKGSPAQPTEYYLFQQMKEDEEEEDTELNEENDPTDIDSIHENVIEGQVYINISPPTTACNSVAASGSSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSESADPSPPPPPRVTSFAEIARNKRRTGGSPSQRSGIEATSTHSHSSGEFSPILEGLPEGQSHSLPPLTRCHSQGSCDLSSSYRPWSTPLRESPGGMGRQTRSKAEGGLSSSTDSSPTVIRYSKDQRPTSLPIQPFTFQHQFGKPQAKPILPLLDGYISQMQSRGAAAPEGDEDDSEEHHRPAHGTSNTPTTVRPSPLGSYSPIRLQGAPTSSGNCSTCSPTLNGPRPPRSLSCPISAGLLPQHTPPGVAIHTETPKLAPLPPTPPPAPLMKQSPLMPALPTRNHCFHRGNLPALPSSGLSPLGQLEPAPQEEPVKVLPACVTQRQPNGQSLRQLPQCYSDFLPDYFSQTERPPEEFCLSPDAHTDESISIDLLQKRGLVKAINTAVDLIVAHFGTSRDAGVKAKLGNSSVSPNVGHLILKYLCPAIREILNDGLKAYVLDLIIGQRKNQPWSVVEASTQLGPSTRVLHSLFSKVSQYSELTNHSMRLNAFIFGLLNLRSLEFWFNHIYTHEDIIAAHYHPWGFLPLSQGACQPLFEELMLLLQPLSLLPFDLDLLFEPHQLQKGEEHLRRKEQLCSARQSLDQSDRSTFQLMRGCGMLESQETGMLPQRESFVLRDEECRLRTEGVTLKMKQEWQRSVGAEREFRNKEDGVGKECLRKSDTGQMQGGGCCAARMKGVGEDNEKDKKREKDGDATKQRNRQAGWWYQLMQSSQVYIDNSAEGSKFVKWEKRRKGGVENHRQSHPPPREGVVEGAEAIQQMDEQVEHGRTSSSNINSIGRGVLNQSALSEPTKATKAKPSWMGSPPESVLTELKKSKEKQPDCQDPNEGLQAQPEAEEDGTAQVLRWGRLFGAGNASKKEKIEQKSVRKQRLPSGWLSIDHSVLDLVAQSVGVRKRAEQQSFTSQSQESNLGPTEQAQTQSPQTKTLAPREVKALCHHIATEPGQLSFNKGDVLQVLSRADSDWLMCALGDSQGLVPIIYVTLIEDSQER

Target ORF information:

RefSeq Version	XM_005165191.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165191.4

ORF Insert Sequence:

ATGGACAGTC CACCTAAGCT TGCAGGAGAG ACTCTTATTG TCCACCACAT TCCGCTGGTT 
CACTGCCAGG TTTCTAGTTC ACGGAAGTGC TGTGGTAGTT CATTAAAAAG GAACAGTAAT 
CCTTTCAGCT GTCCGGAGAA CCTGGGATTG AGTCGGACGA CCTCCCTACC TGAGAGAGAC 
ATTCTTCAGC GGGAGGCACT GGTGTACAGC AGTCTCATAC AGACCTCAAG CAGCAGCCTC 
AGCTCTAGTG ACACTTTTGG GGATGGGGGG ATGAGAGTAG GAGAGAAACG AGGAAGGGGA 
GGAAGGGAAG GGAGTATGGC AAGTGACACT TCCTCTTTCA CCTCAAGCAA TTCTGAAGAT 
CATGCTCAAA GCCTTTCGAT GAAGACACAT GAACGGCCAA GGCGCAACCC CTTTCTTCTC 
AACACTGAAG ATGAAGATGA TGAGGATGAA GACAATCTAA ATGGATATTT GGAAGACTCC 
TCTTTTCACT TGCATGGAAA TTTGCATGAA ATCTCGAATG CAATGCTTTC AAATGAAGAC 
CTGCCACCTT TCCACCTACA TGACCTTGGG TTCACTGCTG AACCTTTTCT CTTGCATGAG 
TCTACAGAAA AACAGTGGTG TTCAACAAAT GGACCAAGTA GGGAAGCAGA GAGTAGAGAC 
CATAAAAGTG GGACTTTTGA TTTGTCTACT CACATGGAGG GTCTGAACAT GCTGAGATTA 
GATAGCCAGA GACGCCATGG AAGCAGTGGT TCAACCCTTT CTATGGACTG TGGTGAGCAG 
GAATGGGGTG AGGATGATGA TTATGAGGAC CACTCCATGC AGGGGGGAAG AGGCAGCTCA 
GAGTCTTCCA GTCTGGCAGC ACCACACTGC TCATGCTGTG GCCTTTCCCA ACCATACTCT 
GAACCTTTTC AGGAATTTTC TGAGTGTCAC CAGGGCTATG GCAGTGATTC CTCCTGCAAT 
AGCTCAGATG GAGTACTGGT TAACTTCAGC ACGATTTACA ACAAGATGAA CAATGTTGTC 
CCAGCTAAGC AGTCCCTAAA TATCAACAGT TCCACTGAGC AATCCTGTGC CTCATCTGTG 
TCTGAGCTTG TTGGGATGTG CGGGACAGAA TGTAGCAGTG GACGTGGCGC TTTCTACTTA 
GACCTTCACA CCTCCCCAGT CGAACCCCCT CAGCATCCTT TAGCCACACA AGAACATGCA 
AACTCTTCCT CTATATCTCA GCAACAAGGT GCCACTTTGG AAGTAGATGC CAACTGCAAC 
TCCTACCATA ACATTCTTGG AAGTGAGGGA CTGTCGTCTG CTGAAACCTC TGCCAATGAC 
CTCACTTCGT GTCTTCAAAG CCAAGCTCGC TTGGTTGTAG CAACACAGAA TTACTACAAG 
CTTGTGACAT GTGACCTTTC TTCCCAGTCC TCTCCCAGTC CTGTGGGATC ATCGGTCACA 
AGCTGTTCAG ATGAGCACAG CAAAGGGAGT CCCGCTCAAC CAACAGAGTA CTACTTATTT 
CAGCAGATGA AAGAGGATGA AGAAGAGGAA GACACAGAAC TGAATGAAGA AAATGACCCA 
ACTGACATTG ACTCTATTCA TGAGAATGTA ATTGAGGGGC AGGTTTATAT CAACATCTCT 
CCACCTACAA CAGCCTGTAA TTCAGTAGCT GCTTCGGGGA GCAGTCGACC TCGCTCTCGA 
AGCTATGATC GGAACTTGGA CAAGTCTCCA TCTCCTCGTC TTGGATCCTT GGAGCGTATG 
CTCAGCTGCC CTGTTCGGCT CAGTGAGAGT GCAGACCCAA GCCCGCCACC TCCACCACGT 
GTTACTTCCT TTGCTGAAAT TGCAAGGAAT AAGAGAAGAA CCGGAGGATC GCCATCTCAG 
AGGAGTGGGA TCGAGGCCAC GTCCACACAC TCTCATTCCT CTGGAGAGTT CTCGCCCATC 
CTGGAAGGCC TTCCTGAGGG TCAGAGCCAT AGCTTGCCAC CTCTGACTCG ATGTCATAGT 
CAGGGGAGCT GTGATTTGTC CTCTTCATAT AGACCATGGA GTACGCCACT GCGAGAATCT 
CCAGGGGGAA TGGGAAGACA GACTCGATCC AAAGCAGAAG GTGGCCTGTC TAGCTCGACG 
GACTCCTCTC CAACTGTAAT TCGCTATAGC AAAGATCAAC GCCCAACCTC TTTACCTATC 
CAGCCCTTCA CTTTTCAGCA CCAGTTTGGG AAACCCCAGG CAAAGCCTAT CCTTCCTCTT 
CTGGACGGAT ACATAAGCCA AATGCAGAGT CGAGGGGCTG CAGCACCAGA GGGTGATGAA 
GATGACTCTG AAGAACACCA CAGGCCAGCT CACGGCACCT CCAACACACC TACAACAGTG 
CGGCCATCTC CACTGGGAAG CTATTCCCCA ATTCGGCTCC AGGGTGCACC TACCTCTTCT 
GGAAATTGCT CAACATGCTC TCCGACTCTG AATGGTCCAC GGCCACCACG TTCCCTCTCT 
TGCCCCATCT CTGCAGGTCT TCTGCCTCAG CACACCCCAC CTGGAGTGGC CATACATACA 
GAGACTCCTA AACTTGCTCC ATTGCCTCCA ACACCACCTC CAGCTCCCCT CATGAAGCAG 
AGTCCATTGA TGCCTGCGTT GCCCACTAGA AATCACTGCT TCCACCGGGG AAATTTGCCA 
GCGCTTCCCA GCTCTGGTCT AAGTCCTCTT GGACAGTTGG AGCCTGCACC GCAGGAAGAA 
CCAGTAAAGG TTTTACCGGC ATGCGTGACA CAGCGACAGC CTAATGGTCA GTCATTGAGG 
CAGCTGCCGC AGTGCTACAG CGACTTCCTG CCTGATTATT TCTCTCAGAC AGAGCGCCCT 
CCTGAGGAGT TCTGCTTATC TCCTGACGCT CATACTGATG AATCCATCTC TATAGACCTG 
TTGCAGAAAA GAGGTTTGGT GAAAGCCATC AACACTGCAG TCGATCTGAT AGTTGCTCAT 
TTTGGCACCA GTCGGGATGC AGGTGTTAAG GCCAAATTGG GTAATAGCTC AGTTAGTCCG 
AATGTGGGTC ACCTTATTCT GAAGTACTTG TGTCCGGCCA TTCGGGAGAT ACTGAATGAT 
GGACTGAAAG CATACGTACT GGACCTGATC ATTGGACAAC GCAAAAACCA GCCCTGGAGT 
GTGGTTGAAG CCTCCACACA ACTTGGACCT TCTACACGAG TTTTGCACAG CCTGTTTTCT 
AAAGTGAGCC AGTATTCTGA GCTGACCAAT CACAGTATGA GACTCAATGC CTTCATCTTT 
GGCCTGCTAA ACTTGCGATC TCTGGAATTC TGGTTCAATC ACATTTATAC GCATGAAGAC 
ATCATAGCAG CACATTACCA CCCATGGGGT TTCCTACCGC TGTCTCAAGG AGCTTGCCAG 
CCTCTTTTTG AAGAGTTGAT GCTCTTGCTT CAGCCACTGT CACTGCTTCC CTTTGACCTT 
GACCTGCTAT TTGAGCCACA CCAGCTTCAG AAAGGAGAAG AACACCTGAG ACGCAAAGAA 
CAGCTGTGCT CTGCTCGCCA GAGCCTCGAC CAATCAGATC GCTCCACTTT CCAACTCATG 
AGAGGCTGTG GAATGTTGGA GTCTCAGGAA ACGGGAATGC TGCCTCAGAG AGAAAGCTTC 
GTGCTGAGAG ATGAGGAGTG CCGGCTTAGG ACTGAGGGAG TGACATTAAA AATGAAACAA 
GAATGGCAGA GGAGTGTGGG AGCAGAACGT GAGTTTCGGA ATAAGGAGGA TGGAGTTGGT 
AAAGAGTGCT TGCGAAAGAG TGACACAGGA CAGATGCAAG GTGGAGGGTG CTGTGCTGCC 
AGAATGAAGG GAGTTGGAGA AGACAATGAG AAGGACAAAA AGAGAGAAAA AGATGGAGAT 
GCAACCAAAC AGAGAAACCG ACAAGCTGGC TGGTGGTATC AGTTGATGCA AAGCTCACAG 
GTTTACATTG ACAACTCTGC CGAGGGGTCG AAATTTGTCA AGTGGGAGAA GAGGAGGAAA 
GGAGGGGTTG AGAATCACCG TCAAAGCCAT CCACCTCCTA GAGAAGGTGT TGTTGAAGGA 
GCTGAGGCCA TTCAGCAGAT GGATGAACAG GTGGAACACG GTCGGACCAG TAGTAGCAAT 
ATTAATAGCA TTGGCCGTGG GGTTCTAAAC CAATCAGCTT TGTCAGAACC CACCAAAGCC 
ACAAAAGCGA AGCCATCTTG GATGGGAAGT CCACCGGAGT CAGTGCTCAC TGAGCTGAAA 
AAGAGTAAGG AGAAGCAGCC AGATTGCCAG GATCCGAATG AAGGGCTTCA GGCCCAGCCA 
GAAGCAGAGG AGGATGGAAC GGCTCAAGTG CTCCGCTGGG GACGACTATT TGGAGCTGGA 
AATGCAAGCA AGAAAGAAAA GATTGAACAG AAATCAGTCA GGAAACAAAG ATTGCCTTCA 
GGTTGGTTGA GTATCGATCA TTCTGTGTTG GATCTGGTGG CTCAGTCTGT TGGAGTTCGG 
AAAAGAGCGG AGCAACAATC TTTTACATCT CAAAGCCAAG AATCCAACCT TGGCCCAACT 
GAACAAGCAC AAACACAGAG TCCCCAAACA AAGACACTAG CCCCACGGGA AGTGAAGGCG 
TTATGCCATC ATATTGCCAC AGAGCCAGGG CAGCTCAGTT TCAACAAAGG TGACGTGCTG 
CAGGTGCTCA GCAGGGCAGA CTCTGATTGG CTGATGTGTG CTCTGGGCGA TAGCCAGGGC 
CTTGTTCCCA TCATATACGT CACCCTGATT GAAGACAGTC AGGAGCGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rusc2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

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