cacna1ba cDNA ORF clone, Danio rerio(zebrafish)

The following cacna1ba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1ba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa142639 XM_021476173.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa142640 XM_021476174.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa142641 XM_021476175.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa142642 XM_021476176.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa142643 XM_021476178.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa142644 XM_021476179.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa142645 XM_021476180.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa142646 XM_021476181.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa142647 XM_021476182.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa59085 XM_017356095.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa59086 XM_017356096.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa59087 XM_017356097.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa36125 NM_001114548.1
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa42256 XM_009301492.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42257 XM_009301493.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42258 XM_009301494.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42259 XM_009301495.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42260 XM_009301496.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42261 XM_009301497.3
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42262 XM_009301498.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42263 XM_009301499.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42264 XM_009301500.3
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42265 XM_009301501.3
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42266 XM_009301503.2
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa42267 XM_009301504.3
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa42268 XM_009301505.3
Latest version!
Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
ODa42267 XM_009301504.2 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa59085 XM_017356095.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa42261 XM_009301497.2 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42265 XM_009301501.2 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42268 XM_009301505.2 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa59086 XM_017356096.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa42264 XM_009301500.2 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa59087 XM_017356097.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa142639
    Clone ID Related Accession (Same CDS sequence) XM_021476173.1
    Accession Version XM_021476173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC
    AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC
    ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT
    CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT
    GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT
    CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT
    CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG
    TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC
    CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGCGGA
    CGGATTTCCT ATAAGGATAT GTACAATTTG TTGCGAATTA TCTCGCCCCC CCTTGGCTTA
    GGGAAGAACT GCCCTAATAG GGTAGCATAC AAGCGTCTGG TGAAAATGAA CATGCCCATT
    GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG
    GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG
    GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA
    CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT
    TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA
    GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC
    ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA
    CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT
    TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT
    AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA
    AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA
    GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA
    ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA
    CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC
    CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG
    AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT
    GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA
    GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC
    AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC
    AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT
    CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC
    TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC
    CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC
    TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC
    CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC
    CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT
    CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC
    AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331848.1
    CDS1..7137
    Translation

    Target ORF information:

    RefSeq Version XM_021476173.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
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    2881
    2941
    3001
    3061
    3121
    3181
    3241
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    3601
    3661
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    3781
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    4141
    4201
    4261
    4321
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    4681
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    5161
    5221
    5281
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    5461
    5521
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    5641
    5701
    5761
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    5881
    5941
    6001
    6061
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    6181
    6241
    6301
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    6541
    6601
    6661
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    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC
    AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC
    ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT
    CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT
    GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT
    CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT
    CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG
    TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC
    CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGCGGA
    CGGATTTCCT ATAAGGATAT GTACAATTTG TTGCGAATTA TCTCGCCCCC CCTTGGCTTA
    GGGAAGAACT GCCCTAATAG GGTAGCATAC AAGCGTCTGG TGAAAATGAA CATGCCCATT
    GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG
    GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG
    GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA
    CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT
    TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA
    GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC
    ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA
    CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT
    TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT
    AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA
    AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA
    GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA
    ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA
    CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC
    CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG
    AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT
    GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA
    GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC
    AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC
    AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT
    CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC
    TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC
    CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC
    TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC
    CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC
    CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT
    CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC
    AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142640
    Clone ID Related Accession (Same CDS sequence) XM_021476174.1
    Accession Version XM_021476174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1501
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    1921
    1981
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    4141
    4201
    4261
    4321
    4381
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    4681
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    4981
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    5161
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    5281
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    6421
    6481
    6541
    6601
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    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACGAGC GTCAGCTGAA CTTCAGTTTC
    CTGAGGCTCT TTAGAGCAGC CCGACTCATC AAGCTCCTCC GGCAGGGATA CACCATTCGC
    ATCCTGCTCT GGACATTTGT CCAGTCTTTT AAGGCACTGC CTTATGTTTG CCTTCTAATC
    GCCATGCTGT TTTTCATCTA CGCCATTATT GGGATGCAGG TGTTTGGAAA CATTGATTTG
    AATGATGACA CGGCAATCAA CCGTCACAAC AACTTCCGCA CTTTCCTCCA AGCTCTTATG
    CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT TGGCATGATA TCATGCTGTC GTGTCTGAGC
    GAGCGCACCT GTGATCCGAG CTCAGGCACC CTTGGAAAGG AGTGTGGGAG TGACTTTGCC
    TACTTCTACT TTGTCTCCTT CATTTTCCTC TGCTCATTTC TGATGTTGAA CCTGTTTGTG
    GCCGTCATTA TGGACAACTT TGAATATCTG ACACGCGACT CCTCCATCCT GGGCCCTCAC
    CACCTGGATG AGTTTATTCG AGTGTGGGCG GAGTATGACC CAGCCGCATG TGGCCGTATC
    AAGTACCTCG ATATGTATGA GATGCTGCTC CACATGTCCC CACCTTTAGG TTTGGGAAAG
    AAATGTCCTC CACGAATCGC CTACAAGCGT CTGGTGAAAA TGAACATGCC CATTGCGGAT
    GACAACAGTG TACACTTTAC CTCAACGCTG ATGGCTCTGA TCCGTACTGC CCTGGAGATC
    AAACTGGCTT CAGGCATGGT GGCTCAGCGT CTGTCAGATG CTGAACTGAA GAAGGAGCTC
    TCTACAGTTT GGCCAAACCT GTCTCAGAAG ACCATGGACC TGCTGGTCAC TCCACATAAA
    CCTAATGAGC TCACAGTGGG GAAGGTGTAT GCAGCTCTTA TGATCTTTGA CTATTACAAG
    CAGAACAGAG CAAAAAGACT TCAGATGCAG CAACAGCAGC TGAAAGAACA GGCAGGACCT
    GGATCTCAGA ATAAAGTCAG CGCTCTCTTA GAGCCCATTC TCCCACTAAC CCACATGCAA
    GATCAGCCAG TGAACGGTGT CGATTCAGCT TCAGAGTGTC AAGCTCAGAC CAGACCCAGC
    TCCACCACAC TCAACAATGG ACGCACAACT CTTGACAACA CAATAAAAGG GTCTTCATCC
    TGGGCCTCAG AGAAATCCAA GGAAGTCCAA AAGTCTAAAC GGAGACCAAT GTCTAGAGGT
    CAATCTGAGG ATGCCTCAAA TGCAAACACT GCCCAGGAGT CAGTAGAAAT GAGAAAGATG
    GAGAACAGTG CCAACACAGT GACCTCTGGT GGCCTTGAGG GCCAAGGACG TGCAGCGTCC
    ATGCCAAAAC TCAATGCTGA GATGCAGAGG AGTCATTCCC GTCAATCTCC TGGAACATTA
    CTGGAACCCA TTCCAGATTC CCCCATGAGG CGCTCTGCAT CCACTTTTGC ACCACAGCGC
    CCTCACGAGG TGAATCTGAA TGACTACATT CTGGAGAAAC CTGTTCAGGA ACGCCATCAC
    CACCATCACC ATCATCACCG ATGCCATCAT CGCCGGGACA GAGACAAGAA ACAGAGATCT
    CTGGACAGGT CGCCTACTGG CCATCACAAA ACTACAGGCA CAGCCACTGA CTCTGTAGAG
    GAGAGGGCAC ATGACCGGGG TCGTTCCCAT GAGAGGAAGC ACCATTCATC TTCAGGAGAG
    AAGCAGCGGT ATTACTCGTG TGATCGCTAT GCCAGTCGGG AACACTGTTC CAGCAAATCT
    GCCGTGGCAA GCTGTGCCAC CTCACCCAGC GAGACCCAGG AGGCCAATCT TAACAAGCAG
    GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG TTGTTGACTT CAGGGGCGAG CACTCCAAGC
    CGCGGGCGCA GACAGCTGCC CCAGACCCCC CTCACTCCTC GTCCCTCCGT GGCCTACAGG
    ACTGCCAACT CTTCCCCTGT GCAACTTCTG GCCACAACGA CCAGCTTGCT TCCCCCGAGT
    CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG CACAACGCTC TTTTACTCAG CGGCTCTATC
    TCCAGCTCGC CGGCCCCCGT CAGTCGCATT AACTCAGAAC CCTTCTTGGG GCCCCCGGAC
    CACCCTGCTG GCTTGCAGCC CGAAGCCTTC GCTGTCTTCC AAGAGAATCC GAGCCCTCAG
    ATGGGCCGTT CTCCTCAGAC TGTAGGGATG CCCTTCGTGG CGATCCCCCC ACCTCCCCCA
    CAGCAGTTTC GCAGGGTCCC CAACGGGTAC CACTTCTCCA CGGGCCCCAG CGCCAGCAGA
    GGACGGGCAC AGCAGTACTA CCCAGAGGCA GAGGTGGAGG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331849.1
    CDS1..7131
    Translation

    Target ORF information:

    RefSeq Version XM_021476174.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACGAGC GTCAGCTGAA CTTCAGTTTC
    CTGAGGCTCT TTAGAGCAGC CCGACTCATC AAGCTCCTCC GGCAGGGATA CACCATTCGC
    ATCCTGCTCT GGACATTTGT CCAGTCTTTT AAGGCACTGC CTTATGTTTG CCTTCTAATC
    GCCATGCTGT TTTTCATCTA CGCCATTATT GGGATGCAGG TGTTTGGAAA CATTGATTTG
    AATGATGACA CGGCAATCAA CCGTCACAAC AACTTCCGCA CTTTCCTCCA AGCTCTTATG
    CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT TGGCATGATA TCATGCTGTC GTGTCTGAGC
    GAGCGCACCT GTGATCCGAG CTCAGGCACC CTTGGAAAGG AGTGTGGGAG TGACTTTGCC
    TACTTCTACT TTGTCTCCTT CATTTTCCTC TGCTCATTTC TGATGTTGAA CCTGTTTGTG
    GCCGTCATTA TGGACAACTT TGAATATCTG ACACGCGACT CCTCCATCCT GGGCCCTCAC
    CACCTGGATG AGTTTATTCG AGTGTGGGCG GAGTATGACC CAGCCGCATG TGGCCGTATC
    AAGTACCTCG ATATGTATGA GATGCTGCTC CACATGTCCC CACCTTTAGG TTTGGGAAAG
    AAATGTCCTC CACGAATCGC CTACAAGCGT CTGGTGAAAA TGAACATGCC CATTGCGGAT
    GACAACAGTG TACACTTTAC CTCAACGCTG ATGGCTCTGA TCCGTACTGC CCTGGAGATC
    AAACTGGCTT CAGGCATGGT GGCTCAGCGT CTGTCAGATG CTGAACTGAA GAAGGAGCTC
    TCTACAGTTT GGCCAAACCT GTCTCAGAAG ACCATGGACC TGCTGGTCAC TCCACATAAA
    CCTAATGAGC TCACAGTGGG GAAGGTGTAT GCAGCTCTTA TGATCTTTGA CTATTACAAG
    CAGAACAGAG CAAAAAGACT TCAGATGCAG CAACAGCAGC TGAAAGAACA GGCAGGACCT
    GGATCTCAGA ATAAAGTCAG CGCTCTCTTA GAGCCCATTC TCCCACTAAC CCACATGCAA
    GATCAGCCAG TGAACGGTGT CGATTCAGCT TCAGAGTGTC AAGCTCAGAC CAGACCCAGC
    TCCACCACAC TCAACAATGG ACGCACAACT CTTGACAACA CAATAAAAGG GTCTTCATCC
    TGGGCCTCAG AGAAATCCAA GGAAGTCCAA AAGTCTAAAC GGAGACCAAT GTCTAGAGGT
    CAATCTGAGG ATGCCTCAAA TGCAAACACT GCCCAGGAGT CAGTAGAAAT GAGAAAGATG
    GAGAACAGTG CCAACACAGT GACCTCTGGT GGCCTTGAGG GCCAAGGACG TGCAGCGTCC
    ATGCCAAAAC TCAATGCTGA GATGCAGAGG AGTCATTCCC GTCAATCTCC TGGAACATTA
    CTGGAACCCA TTCCAGATTC CCCCATGAGG CGCTCTGCAT CCACTTTTGC ACCACAGCGC
    CCTCACGAGG TGAATCTGAA TGACTACATT CTGGAGAAAC CTGTTCAGGA ACGCCATCAC
    CACCATCACC ATCATCACCG ATGCCATCAT CGCCGGGACA GAGACAAGAA ACAGAGATCT
    CTGGACAGGT CGCCTACTGG CCATCACAAA ACTACAGGCA CAGCCACTGA CTCTGTAGAG
    GAGAGGGCAC ATGACCGGGG TCGTTCCCAT GAGAGGAAGC ACCATTCATC TTCAGGAGAG
    AAGCAGCGGT ATTACTCGTG TGATCGCTAT GCCAGTCGGG AACACTGTTC CAGCAAATCT
    GCCGTGGCAA GCTGTGCCAC CTCACCCAGC GAGACCCAGG AGGCCAATCT TAACAAGCAG
    GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG TTGTTGACTT CAGGGGCGAG CACTCCAAGC
    CGCGGGCGCA GACAGCTGCC CCAGACCCCC CTCACTCCTC GTCCCTCCGT GGCCTACAGG
    ACTGCCAACT CTTCCCCTGT GCAACTTCTG GCCACAACGA CCAGCTTGCT TCCCCCGAGT
    CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG CACAACGCTC TTTTACTCAG CGGCTCTATC
    TCCAGCTCGC CGGCCCCCGT CAGTCGCATT AACTCAGAAC CCTTCTTGGG GCCCCCGGAC
    CACCCTGCTG GCTTGCAGCC CGAAGCCTTC GCTGTCTTCC AAGAGAATCC GAGCCCTCAG
    ATGGGCCGTT CTCCTCAGAC TGTAGGGATG CCCTTCGTGG CGATCCCCCC ACCTCCCCCA
    CAGCAGTTTC GCAGGGTCCC CAACGGGTAC CACTTCTCCA CGGGCCCCAG CGCCAGCAGA
    GGACGGGCAC AGCAGTACTA CCCAGAGGCA GAGGTGGAGG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142641
    Clone ID Related Accession (Same CDS sequence) XM_021476175.1
    Accession Version XM_021476175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCATCAC ATCAAAGCGT AAACAGAGGA ACCAAGGGCA AAGACATCAG CACTATCAAG
    TCACTGAGGG TGTTGCGAGT CTTGCGACCT CTGAAGACCA TCAAACGTCT ACCAAAACTG
    AAGGCCGTGT TTGATTGTGT GGTGAACTCC CTGAAGAATG TCCTGAATAT CCTCATTGTC
    TACATTCTGT TCATGTTCAT TTTCGCTGTC ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT
    TTTTACTGCA CAGACGAGTC CAAGGGCCTG GAGAAAGACT GCAGGGGGCT GTTTTTGGAT
    TATGATAAGG ATGATGTGAC GGCACAACCG AGGGAATGGA AGAAATACGA GTTTCACTAT
    GACAATGTGC TTTGGGCTTT TCTCACTCTC TTCACTGTTT CTACGGGAGA GGGCTGGCCT
    ACGGTTTTGA AGCACTCCGT GGATGCCACT TTTGAGGATC AGGGTCCCAG TCCAGGCTAC
    CGCATTGAGA TGTCTATTTT TTACGTGGTC TACTTTGTGG TTTTCCCTTT CTTCTTCGTC
    AACATTTTTG TAGCCTTGAT AATCATCACA TTCCAGGAAC AGGGTGATAA GGTCTTGTCA
    GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC TGCATCGACT TTGCTATCAA TGCCAAACCT
    CTGACACGCT ACATGCCGCA GAACCAGCAG TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG
    GTCTCTCCGC CGTTTGAGTA CTCCATCATG ATCATGATTG CTCTCAACAC TGTGGTGCTC
    ATGATGAAGT TCCATGGGGC GCCTGACTTT TATGAGGCAA TGCTTAAGTA TCTCAACATT
    GTCTTCACTG TTCTCTTTTC ACTGGAGTGT ATTCTCAAGA TTATTGCTTT CGGCCCCTTG
    AATTACCTGA AAGATGCCTG GAATGTGTTT GACTTTGTGA CTGTGCTGGG CAGCATCACT
    GACATTTTAG TCACTGAGAT TAACACAACG GAGCGTCAGC TGAACTTCAG TTTCCTGAGG
    CTCTTTAGAG CAGCCCGACT CATCAAGCTC CTCCGGCAGG GATACACCAT TCGCATCCTG
    CTCTGGACAT TTGTCCAGTC TTTTAAGGCA CTGCCTTATG TTTGCCTTCT AATCGCCATG
    CTGTTTTTCA TCTACGCCAT TATTGGGATG CAGGTGTTTG GAAACATTGA TTTGAATGAT
    GACACGGCAA TCAACCGTCA CAACAACTTC CGCACTTTCC TCCAAGCTCT TATGCTTTTG
    TTCAGGAGTG CCACTGGCGA GGCTTGGCAT GATATCATGC TGTCGTGTCT GAGCGAGCGC
    ACCTGTGATC CGAGCTCAGG CACCCTTGGA AAGGAGTGTG GGAGTGACTT TGCCTACTTC
    TACTTTGTCT CCTTCATTTT CCTCTGCTCA TTTCTGATGT TGAACCTGTT TGTGGCCGTC
    ATTATGGACA ACTTTGAATA TCTGACACGC GACTCCTCCA TCCTGGGCCC TCACCACCTG
    GATGAGTTTA TTCGAGTGTG GGCGGAGTAT GACCCAGCCG CATGTGGCCG TATCAAGTAC
    CTCGATATGT ATGAGATGCT GCTCCACATG TCCCCACCTT TAGGTTTGGG AAAGAAATGT
    CCTCCACGAA TCGCCTACAA GCGTCTGGTG AAAATGAACA TGCCCATTGC GGATGACAAC
    AGTGTACACT TTACCTCAAC GCTGATGGCT CTGATCCGTA CTGCCCTGGA GATCAAACTG
    GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA GATGCTGAAC TGAAGAAGGA GCTCTCTACA
    GTTTGGCCAA ACCTGTCTCA GAAGACCATG GACCTGCTGG TCACTCCACA TAAACCTAAT
    GAGCTCACAG TGGGGAAGGT GTATGCAGCT CTTATGATCT TTGACTATTA CAAGCAGAAC
    AGAGCAAAAA GACTTCAGAT GCAGCAACAG CAGCTGAAAG AACAGGCAGG ACCTGGATCT
    CAGAATAAAG TCAGCGCTCT CTTAGAGCCC ATTCTCCCAC TAACCCACAT GCAAGATCAG
    CCAGTGAACG GTGTCGATTC AGCTTCAGAG TGTCAAGCTC AGACCAGACC CAGCTCCACC
    ACACTCAACA ATGGACGCAC AACTCTTGAC AACACAATAA AAGGGTCTTC ATCCTGGGCC
    TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT AAACGGAGAC CAATGTCTAG AGGTCAATCT
    GAGGATGCCT CAAATGCAAA CACTGCCCAG GAGTCAGTAG AAATGAGAAA GATGGAGAAC
    AGTGCCAACA CAGTGACCTC TGGTGGCCTT GAGGGCCAAG GACGTGCAGC GTCCATGCCA
    AAACTCAATG CTGAGATGCA GAGGAGTCAT TCCCGTCAAT CTCCTGGAAC ATTACTGGAA
    CCCATTCCAG ATTCCCCCAT GAGGCGCTCT GCATCCACTT TTGCACCACA GCGCCCTCAC
    GAGGTGAATC TGAATGACTA CATTCTGGAG AAACCTGTTC AGGAACGCCA TCACCACCAT
    CACCATCATC ACCGATGCCA TCATCGCCGG GACAGAGACA AGAAACAGAG ATCTCTGGAC
    AGGTCGCCTA CTGGCCATCA CAAAACTACA GGCACAGCCA CTGACTCTGT AGAGGAGAGG
    GCACATGACC GGGGTCGTTC CCATGAGAGG AAGCACCATT CATCTTCAGG AGAGAAGCAG
    CGGTATTACT CGTGTGATCG CTATGCCAGT CGGGAACACT GTTCCAGCAA ATCTGCCGTG
    GCAAGCTGTG CCACCTCACC CAGCGAGACC CAGGAGGCCA ATCTTAACAA GCAGGGCAGT
    GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG ACTTCAGGGG CGAGCACTCC AAGCCGCGGG
    CGCAGACAGC TGCCCCAGAC CCCCCTCACT CCTCGTCCCT CCGTGGCCTA CAGGACTGCC
    AACTCTTCCC CTGTGCAACT TCTGGCCACA ACGACCAGCT TGCTTCCCCC GAGTCCCGGC
    AGGCTGAGCC GTGGTCTGTC CGAGCACAAC GCTCTTTTAC TCAGCGGCTC TATCTCCAGC
    TCGCCGGCCC CCGTCAGTCG CATTAACTCA GAACCCTTCT TGGGGCCCCC GGACCACCCT
    GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC TTCCAAGAGA ATCCGAGCCC TCAGATGGGC
    CGTTCTCCTC AGACTGTAGG GATGCCCTTC GTGGCGATCC CCCCACCTCC CCCACAGCAG
    TTTCGCAGGG TCCCCAACGG GTACCACTTC TCCACGGGCC CCAGCGCCAG CAGAGGACGG
    GCACAGCAGT ACTACCCAGA GGCAGAGGTG GAGGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331850.1
    CDS1..7125
    Translation

    Target ORF information:

    RefSeq Version XM_021476175.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCATCAC ATCAAAGCGT AAACAGAGGA ACCAAGGGCA AAGACATCAG CACTATCAAG
    TCACTGAGGG TGTTGCGAGT CTTGCGACCT CTGAAGACCA TCAAACGTCT ACCAAAACTG
    AAGGCCGTGT TTGATTGTGT GGTGAACTCC CTGAAGAATG TCCTGAATAT CCTCATTGTC
    TACATTCTGT TCATGTTCAT TTTCGCTGTC ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT
    TTTTACTGCA CAGACGAGTC CAAGGGCCTG GAGAAAGACT GCAGGGGGCT GTTTTTGGAT
    TATGATAAGG ATGATGTGAC GGCACAACCG AGGGAATGGA AGAAATACGA GTTTCACTAT
    GACAATGTGC TTTGGGCTTT TCTCACTCTC TTCACTGTTT CTACGGGAGA GGGCTGGCCT
    ACGGTTTTGA AGCACTCCGT GGATGCCACT TTTGAGGATC AGGGTCCCAG TCCAGGCTAC
    CGCATTGAGA TGTCTATTTT TTACGTGGTC TACTTTGTGG TTTTCCCTTT CTTCTTCGTC
    AACATTTTTG TAGCCTTGAT AATCATCACA TTCCAGGAAC AGGGTGATAA GGTCTTGTCA
    GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC TGCATCGACT TTGCTATCAA TGCCAAACCT
    CTGACACGCT ACATGCCGCA GAACCAGCAG TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG
    GTCTCTCCGC CGTTTGAGTA CTCCATCATG ATCATGATTG CTCTCAACAC TGTGGTGCTC
    ATGATGAAGT TCCATGGGGC GCCTGACTTT TATGAGGCAA TGCTTAAGTA TCTCAACATT
    GTCTTCACTG TTCTCTTTTC ACTGGAGTGT ATTCTCAAGA TTATTGCTTT CGGCCCCTTG
    AATTACCTGA AAGATGCCTG GAATGTGTTT GACTTTGTGA CTGTGCTGGG CAGCATCACT
    GACATTTTAG TCACTGAGAT TAACACAACG GAGCGTCAGC TGAACTTCAG TTTCCTGAGG
    CTCTTTAGAG CAGCCCGACT CATCAAGCTC CTCCGGCAGG GATACACCAT TCGCATCCTG
    CTCTGGACAT TTGTCCAGTC TTTTAAGGCA CTGCCTTATG TTTGCCTTCT AATCGCCATG
    CTGTTTTTCA TCTACGCCAT TATTGGGATG CAGGTGTTTG GAAACATTGA TTTGAATGAT
    GACACGGCAA TCAACCGTCA CAACAACTTC CGCACTTTCC TCCAAGCTCT TATGCTTTTG
    TTCAGGAGTG CCACTGGCGA GGCTTGGCAT GATATCATGC TGTCGTGTCT GAGCGAGCGC
    ACCTGTGATC CGAGCTCAGG CACCCTTGGA AAGGAGTGTG GGAGTGACTT TGCCTACTTC
    TACTTTGTCT CCTTCATTTT CCTCTGCTCA TTTCTGATGT TGAACCTGTT TGTGGCCGTC
    ATTATGGACA ACTTTGAATA TCTGACACGC GACTCCTCCA TCCTGGGCCC TCACCACCTG
    GATGAGTTTA TTCGAGTGTG GGCGGAGTAT GACCCAGCCG CATGTGGCCG TATCAAGTAC
    CTCGATATGT ATGAGATGCT GCTCCACATG TCCCCACCTT TAGGTTTGGG AAAGAAATGT
    CCTCCACGAA TCGCCTACAA GCGTCTGGTG AAAATGAACA TGCCCATTGC GGATGACAAC
    AGTGTACACT TTACCTCAAC GCTGATGGCT CTGATCCGTA CTGCCCTGGA GATCAAACTG
    GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA GATGCTGAAC TGAAGAAGGA GCTCTCTACA
    GTTTGGCCAA ACCTGTCTCA GAAGACCATG GACCTGCTGG TCACTCCACA TAAACCTAAT
    GAGCTCACAG TGGGGAAGGT GTATGCAGCT CTTATGATCT TTGACTATTA CAAGCAGAAC
    AGAGCAAAAA GACTTCAGAT GCAGCAACAG CAGCTGAAAG AACAGGCAGG ACCTGGATCT
    CAGAATAAAG TCAGCGCTCT CTTAGAGCCC ATTCTCCCAC TAACCCACAT GCAAGATCAG
    CCAGTGAACG GTGTCGATTC AGCTTCAGAG TGTCAAGCTC AGACCAGACC CAGCTCCACC
    ACACTCAACA ATGGACGCAC AACTCTTGAC AACACAATAA AAGGGTCTTC ATCCTGGGCC
    TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT AAACGGAGAC CAATGTCTAG AGGTCAATCT
    GAGGATGCCT CAAATGCAAA CACTGCCCAG GAGTCAGTAG AAATGAGAAA GATGGAGAAC
    AGTGCCAACA CAGTGACCTC TGGTGGCCTT GAGGGCCAAG GACGTGCAGC GTCCATGCCA
    AAACTCAATG CTGAGATGCA GAGGAGTCAT TCCCGTCAAT CTCCTGGAAC ATTACTGGAA
    CCCATTCCAG ATTCCCCCAT GAGGCGCTCT GCATCCACTT TTGCACCACA GCGCCCTCAC
    GAGGTGAATC TGAATGACTA CATTCTGGAG AAACCTGTTC AGGAACGCCA TCACCACCAT
    CACCATCATC ACCGATGCCA TCATCGCCGG GACAGAGACA AGAAACAGAG ATCTCTGGAC
    AGGTCGCCTA CTGGCCATCA CAAAACTACA GGCACAGCCA CTGACTCTGT AGAGGAGAGG
    GCACATGACC GGGGTCGTTC CCATGAGAGG AAGCACCATT CATCTTCAGG AGAGAAGCAG
    CGGTATTACT CGTGTGATCG CTATGCCAGT CGGGAACACT GTTCCAGCAA ATCTGCCGTG
    GCAAGCTGTG CCACCTCACC CAGCGAGACC CAGGAGGCCA ATCTTAACAA GCAGGGCAGT
    GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG ACTTCAGGGG CGAGCACTCC AAGCCGCGGG
    CGCAGACAGC TGCCCCAGAC CCCCCTCACT CCTCGTCCCT CCGTGGCCTA CAGGACTGCC
    AACTCTTCCC CTGTGCAACT TCTGGCCACA ACGACCAGCT TGCTTCCCCC GAGTCCCGGC
    AGGCTGAGCC GTGGTCTGTC CGAGCACAAC GCTCTTTTAC TCAGCGGCTC TATCTCCAGC
    TCGCCGGCCC CCGTCAGTCG CATTAACTCA GAACCCTTCT TGGGGCCCCC GGACCACCCT
    GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC TTCCAAGAGA ATCCGAGCCC TCAGATGGGC
    CGTTCTCCTC AGACTGTAGG GATGCCCTTC GTGGCGATCC CCCCACCTCC CCCACAGCAG
    TTTCGCAGGG TCCCCAACGG GTACCACTTC TCCACGGGCC CCAGCGCCAG CAGAGGACGG
    GCACAGCAGT ACTACCCAGA GGCAGAGGTG GAGGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142642
    Clone ID Related Accession (Same CDS sequence) XM_021476176.1
    Accession Version XM_021476176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGAGG AACCAAGGGC AAAGACATCA GCACTATCAA GTCACTGAGG
    GTGTTGCGAG TCTTGCGACC TCTGAAGACC ATCAAACGTC TACCAAAACT GAAGGCCGTG
    TTTGATTGTG TGGTGAACTC CCTGAAGAAT GTCCTGAATA TCCTCATTGT CTACATTCTG
    TTCATGTTCA TTTTCGCTGT CATTGCTGTG CAGCTCTTCA AGGGCAAGTT TTTTTACTGC
    ACAGACGAGT CCAAGGGCCT GGAGAAAGAC TGCAGGGGGC TGTTTTTGGA TTATGATAAG
    GATGATGTGA CGGCACAACC GAGGGAATGG AAGAAATACG AGTTTCACTA TGACAATGTG
    CTTTGGGCTT TTCTCACTCT CTTCACTGTT TCTACGGGAG AGGGCTGGCC TACGGTTTTG
    AAGCACTCCG TGGATGCCAC TTTTGAGGAT CAGGGTCCCA GTCCAGGCTA CCGCATTGAG
    ATGTCTATTT TTTACGTGGT CTACTTTGTG GTTTTCCCTT TCTTCTTCGT CAACATTTTT
    GTAGCCTTGA TAATCATCAC ATTCCAGGAA CAGGGTGATA AGGTCTTGTC AGAGTGCAGT
    CTGGAGAAGA ATGAGAGAGC CTGCATCGAC TTTGCTATCA ATGCCAAACC TCTGACACGC
    TACATGCCGC AGAACCAGCA GTCCTTTCAG TACCGCCTGT GGAAGTTTGT GGTCTCTCCG
    CCGTTTGAGT ACTCCATCAT GATCATGATT GCTCTCAACA CTGTGGTGCT CATGATGAAG
    TTCCATGGGG CGCCTGACTT TTATGAGGCA ATGCTTAAGT ATCTCAACAT TGTCTTCACT
    GTTCTCTTTT CACTGGAGTG TATTCTCAAG ATTATTGCTT TCGGCCCCTT GAATTACCTG
    AAAGATGCCT GGAATGTGTT TGACTTTGTG ACTGTGCTGG GCAGCATCAC TGACATTTTA
    GTCACTGAGA TTAACACAAC GGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA
    GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA
    TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC
    ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA
    ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT
    GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT
    CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC
    TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC
    AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT
    ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG
    TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA
    ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC
    TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC
    ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA
    AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA
    GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA
    AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA
    GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC
    GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC
    AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA
    TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC
    TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC
    ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT
    GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA
    GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT
    CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT
    CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT
    ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC
    CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC
    TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT
    GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT
    AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG
    CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC
    CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC
    CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC
    CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG
    CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT
    CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG
    GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG
    TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331851.1
    CDS1..7116
    Translation

    Target ORF information:

    RefSeq Version XM_021476176.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGAGG AACCAAGGGC AAAGACATCA GCACTATCAA GTCACTGAGG
    GTGTTGCGAG TCTTGCGACC TCTGAAGACC ATCAAACGTC TACCAAAACT GAAGGCCGTG
    TTTGATTGTG TGGTGAACTC CCTGAAGAAT GTCCTGAATA TCCTCATTGT CTACATTCTG
    TTCATGTTCA TTTTCGCTGT CATTGCTGTG CAGCTCTTCA AGGGCAAGTT TTTTTACTGC
    ACAGACGAGT CCAAGGGCCT GGAGAAAGAC TGCAGGGGGC TGTTTTTGGA TTATGATAAG
    GATGATGTGA CGGCACAACC GAGGGAATGG AAGAAATACG AGTTTCACTA TGACAATGTG
    CTTTGGGCTT TTCTCACTCT CTTCACTGTT TCTACGGGAG AGGGCTGGCC TACGGTTTTG
    AAGCACTCCG TGGATGCCAC TTTTGAGGAT CAGGGTCCCA GTCCAGGCTA CCGCATTGAG
    ATGTCTATTT TTTACGTGGT CTACTTTGTG GTTTTCCCTT TCTTCTTCGT CAACATTTTT
    GTAGCCTTGA TAATCATCAC ATTCCAGGAA CAGGGTGATA AGGTCTTGTC AGAGTGCAGT
    CTGGAGAAGA ATGAGAGAGC CTGCATCGAC TTTGCTATCA ATGCCAAACC TCTGACACGC
    TACATGCCGC AGAACCAGCA GTCCTTTCAG TACCGCCTGT GGAAGTTTGT GGTCTCTCCG
    CCGTTTGAGT ACTCCATCAT GATCATGATT GCTCTCAACA CTGTGGTGCT CATGATGAAG
    TTCCATGGGG CGCCTGACTT TTATGAGGCA ATGCTTAAGT ATCTCAACAT TGTCTTCACT
    GTTCTCTTTT CACTGGAGTG TATTCTCAAG ATTATTGCTT TCGGCCCCTT GAATTACCTG
    AAAGATGCCT GGAATGTGTT TGACTTTGTG ACTGTGCTGG GCAGCATCAC TGACATTTTA
    GTCACTGAGA TTAACACAAC GGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA
    GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA
    TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC
    ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA
    ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT
    GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT
    CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC
    TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC
    AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT
    ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG
    TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA
    ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC
    TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC
    ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA
    AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA
    GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA
    AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA
    GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC
    GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC
    AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA
    TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC
    TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC
    ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT
    GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA
    GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT
    CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT
    CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT
    ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC
    CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC
    TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT
    GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT
    AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG
    CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC
    CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC
    CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC
    CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG
    CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT
    CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG
    GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG
    TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142643
    Clone ID Related Accession (Same CDS sequence) XM_021476178.1
    Accession Version XM_021476178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACGAGCGTC AGCTGAACTT CAGTTTCCTG AGGCTCTTTA GAGCAGCCCG ACTCATCAAG
    CTCCTCCGGC AGGGATACAC CATTCGCATC CTGCTCTGGA CATTTGTCCA GTCTTTTAAG
    GCACTGCCTT ATGTTTGCCT TCTAATCGCC ATGCTGTTTT TCATCTACGC CATTATTGGG
    ATGCAGGTGT TTGGAAACAT TGATTTGAAT GATGACACGG CAATCAACCG TCACAACAAC
    TTCCGCACTT TCCTCCAAGC TCTTATGCTT TTGTTCAGGA GTGCCACTGG CGAGGCTTGG
    CATGATATCA TGCTGTCGTG TCTGAGCGAG CGCACCTGTG ATCCGAGCTC AGGCACCCTT
    GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT TTTCCTCTGC
    TCATTTCTGA TGTTGAACCT GTTTGTGGCC GTCATTATGG ACAACTTTGA ATATCTGACA
    CGCGACTCCT CCATCCTGGG CCCTCACCAC CTGGATGAGT TTATTCGAGT GTGGGCGGAG
    TATGACCCAG CCGCATGTGG CCGTATCAAG TACCTCGATA TGTATGAGAT GCTGCTCCAC
    ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA TGTCCTCCAC GAATCGCCTA CAAGCGTCTG
    GTGAAAATGA ACATGCCCAT TGCGGATGAC AACAGTGTAC ACTTTACCTC AACGCTGATG
    GCTCTGATCC GTACTGCCCT GGAGATCAAA CTGGCTTCAG GCATGGTGGC TCAGCGTCTG
    TCAGATGCTG AACTGAAGAA GGAGCTCTCT ACAGTTTGGC CAAACCTGTC TCAGAAGACC
    ATGGACCTGC TGGTCACTCC ACATAAACCT AATGAGCTCA CAGTGGGGAA GGTGTATGCA
    GCTCTTATGA TCTTTGACTA TTACAAGCAG AACAGAGCAA AAAGACTTCA GATGCAGCAA
    CAGCAGCTGA AAGAACAGGC AGGACCTGGA TCTCAGAATA AAGTCAGCGC TCTCTTAGAG
    CCCATTCTCC CACTAACCCA CATGCAAGAT CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA
    GAGTGTCAAG CTCAGACCAG ACCCAGCTCC ACCACACTCA ACAATGGACG CACAACTCTT
    GACAACACAA TAAAAGGGTC TTCATCCTGG GCCTCAGAGA AATCCAAGGA AGTCCAAAAG
    TCTAAACGGA GACCAATGTC TAGAGGTCAA TCTGAGGATG CCTCAAATGC AAACACTGCC
    CAGGAGTCAG TAGAAATGAG AAAGATGGAG AACAGTGCCA ACACAGTGAC CTCTGGTGGC
    CTTGAGGGCC AAGGACGTGC AGCGTCCATG CCAAAACTCA ATGCTGAGAT GCAGAGGAGT
    CATTCCCGTC AATCTCCTGG AACATTACTG GAACCCATTC CAGATTCCCC CATGAGGCGC
    TCTGCATCCA CTTTTGCACC ACAGCGCCCT CACGAGGTGA ATCTGAATGA CTACATTCTG
    GAGAAACCTG TTCAGGAACG CCATCACCAC CATCACCATC ATCACCGATG CCATCATCGC
    CGGGACAGAG ACAAGAAACA GAGATCTCTG GACAGGTCGC CTACTGGCCA TCACAAAACT
    ACAGGCACAG CCACTGACTC TGTAGAGGAG AGGGCACATG ACCGGGGTCG TTCCCATGAG
    AGGAAGCACC ATTCATCTTC AGGAGAGAAG CAGCGGTATT ACTCGTGTGA TCGCTATGCC
    AGTCGGGAAC ACTGTTCCAG CAAATCTGCC GTGGCAAGCT GTGCCACCTC ACCCAGCGAG
    ACCCAGGAGG CCAATCTTAA CAAGCAGGGC AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG
    TTGACTTCAG GGGCGAGCAC TCCAAGCCGC GGGCGCAGAC AGCTGCCCCA GACCCCCCTC
    ACTCCTCGTC CCTCCGTGGC CTACAGGACT GCCAACTCTT CCCCTGTGCA ACTTCTGGCC
    ACAACGACCA GCTTGCTTCC CCCGAGTCCC GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC
    AACGCTCTTT TACTCAGCGG CTCTATCTCC AGCTCGCCGG CCCCCGTCAG TCGCATTAAC
    TCAGAACCCT TCTTGGGGCC CCCGGACCAC CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT
    GTCTTCCAAG AGAATCCGAG CCCTCAGATG GGCCGTTCTC CTCAGACTGT AGGGATGCCC
    TTCGTGGCGA TCCCCCCACC TCCCCCACAG CAGTTTCGCA GGGTCCCCAA CGGGTACCAC
    TTCTCCACGG GCCCCAGCGC CAGCAGAGGA CGGGCACAGC AGTACTACCC AGAGGCAGAG
    GTGGAGGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331853.1
    CDS1..7098
    Translation

    Target ORF information:

    RefSeq Version XM_021476178.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACGAGCGTC AGCTGAACTT CAGTTTCCTG AGGCTCTTTA GAGCAGCCCG ACTCATCAAG
    CTCCTCCGGC AGGGATACAC CATTCGCATC CTGCTCTGGA CATTTGTCCA GTCTTTTAAG
    GCACTGCCTT ATGTTTGCCT TCTAATCGCC ATGCTGTTTT TCATCTACGC CATTATTGGG
    ATGCAGGTGT TTGGAAACAT TGATTTGAAT GATGACACGG CAATCAACCG TCACAACAAC
    TTCCGCACTT TCCTCCAAGC TCTTATGCTT TTGTTCAGGA GTGCCACTGG CGAGGCTTGG
    CATGATATCA TGCTGTCGTG TCTGAGCGAG CGCACCTGTG ATCCGAGCTC AGGCACCCTT
    GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT TTTCCTCTGC
    TCATTTCTGA TGTTGAACCT GTTTGTGGCC GTCATTATGG ACAACTTTGA ATATCTGACA
    CGCGACTCCT CCATCCTGGG CCCTCACCAC CTGGATGAGT TTATTCGAGT GTGGGCGGAG
    TATGACCCAG CCGCATGTGG CCGTATCAAG TACCTCGATA TGTATGAGAT GCTGCTCCAC
    ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA TGTCCTCCAC GAATCGCCTA CAAGCGTCTG
    GTGAAAATGA ACATGCCCAT TGCGGATGAC AACAGTGTAC ACTTTACCTC AACGCTGATG
    GCTCTGATCC GTACTGCCCT GGAGATCAAA CTGGCTTCAG GCATGGTGGC TCAGCGTCTG
    TCAGATGCTG AACTGAAGAA GGAGCTCTCT ACAGTTTGGC CAAACCTGTC TCAGAAGACC
    ATGGACCTGC TGGTCACTCC ACATAAACCT AATGAGCTCA CAGTGGGGAA GGTGTATGCA
    GCTCTTATGA TCTTTGACTA TTACAAGCAG AACAGAGCAA AAAGACTTCA GATGCAGCAA
    CAGCAGCTGA AAGAACAGGC AGGACCTGGA TCTCAGAATA AAGTCAGCGC TCTCTTAGAG
    CCCATTCTCC CACTAACCCA CATGCAAGAT CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA
    GAGTGTCAAG CTCAGACCAG ACCCAGCTCC ACCACACTCA ACAATGGACG CACAACTCTT
    GACAACACAA TAAAAGGGTC TTCATCCTGG GCCTCAGAGA AATCCAAGGA AGTCCAAAAG
    TCTAAACGGA GACCAATGTC TAGAGGTCAA TCTGAGGATG CCTCAAATGC AAACACTGCC
    CAGGAGTCAG TAGAAATGAG AAAGATGGAG AACAGTGCCA ACACAGTGAC CTCTGGTGGC
    CTTGAGGGCC AAGGACGTGC AGCGTCCATG CCAAAACTCA ATGCTGAGAT GCAGAGGAGT
    CATTCCCGTC AATCTCCTGG AACATTACTG GAACCCATTC CAGATTCCCC CATGAGGCGC
    TCTGCATCCA CTTTTGCACC ACAGCGCCCT CACGAGGTGA ATCTGAATGA CTACATTCTG
    GAGAAACCTG TTCAGGAACG CCATCACCAC CATCACCATC ATCACCGATG CCATCATCGC
    CGGGACAGAG ACAAGAAACA GAGATCTCTG GACAGGTCGC CTACTGGCCA TCACAAAACT
    ACAGGCACAG CCACTGACTC TGTAGAGGAG AGGGCACATG ACCGGGGTCG TTCCCATGAG
    AGGAAGCACC ATTCATCTTC AGGAGAGAAG CAGCGGTATT ACTCGTGTGA TCGCTATGCC
    AGTCGGGAAC ACTGTTCCAG CAAATCTGCC GTGGCAAGCT GTGCCACCTC ACCCAGCGAG
    ACCCAGGAGG CCAATCTTAA CAAGCAGGGC AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG
    TTGACTTCAG GGGCGAGCAC TCCAAGCCGC GGGCGCAGAC AGCTGCCCCA GACCCCCCTC
    ACTCCTCGTC CCTCCGTGGC CTACAGGACT GCCAACTCTT CCCCTGTGCA ACTTCTGGCC
    ACAACGACCA GCTTGCTTCC CCCGAGTCCC GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC
    AACGCTCTTT TACTCAGCGG CTCTATCTCC AGCTCGCCGG CCCCCGTCAG TCGCATTAAC
    TCAGAACCCT TCTTGGGGCC CCCGGACCAC CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT
    GTCTTCCAAG AGAATCCGAG CCCTCAGATG GGCCGTTCTC CTCAGACTGT AGGGATGCCC
    TTCGTGGCGA TCCCCCCACC TCCCCCACAG CAGTTTCGCA GGGTCCCCAA CGGGTACCAC
    TTCTCCACGG GCCCCAGCGC CAGCAGAGGA CGGGCACAGC AGTACTACCC AGAGGCAGAG
    GTGGAGGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142644
    Clone ID Related Accession (Same CDS sequence) XM_021476179.1
    Accession Version XM_021476179.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACGAGCGTC AGCTGAACTT CAGTTTCCTG AGGCTCTTTA GAGCAGCCCG ACTCATCAAG
    CTCCTCCGGC AGGGATACAC CATTCGCATC CTGCTCTGGA CATTTGTCCA GTCTTTTAAG
    GCACTGCCTT ATGTTTGCCT TCTAATCGCC ATGCTGTTTT TCATCTACGC CATTATTGGG
    ATGCAGGTGT TTGGAAACAT TGATTTGAAT GATGACACGG CAATCAACCG TCACAACAAC
    TTCCGCACTT TCCTCCAAGC TCTTATGCTT TTGTTCAGGA GTGCCACTGG CGAGGCTTGG
    CATGATATCA TGCTGTCGTG TCTGAGCGAG CGCACCTGTG ATCCGAGCTC AGGCACCCTT
    GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT TTTCCTCTGC
    TCATTTCTGA TGTTGAACCT GTTTGTGGCC GTCATTATGG ACAACTTTGA ATATCTGACA
    CGCGACTCCT CCATCCTGGG CCCTCACCAC CTGGATGAGT TTATTCGAGT GTGGGCGGAG
    TATGACCCAG CCGCATGCGG ACGGATTTCC TATAAGGATA TGTACAATTT GTTGCGAATT
    ATCTCGCCCC CCCTTGGCTT AGGGAAGAAC TGCCCTAATA GGGTAGCATA CAAGCGTCTG
    GTGAAAATGA ACATGCCCAT TGCGGATGAC AACAGTGTAC ACTTTACCTC AACGCTGATG
    GCTCTGATCC GTACTGCCCT GGAGATCAAA CTGGCTTCAG GCATGGTGGC TCAGCGTCTG
    TCAGATGCTG AACTGAAGAA GGAGCTCTCT ACAGTTTGGC CAAACCTGTC TCAGAAGACC
    ATGGACCTGC TGGTCACTCC ACATAAACCT AATGAGCTCA CAGTGGGGAA GGTGTATGCA
    GCTCTTATGA TCTTTGACTA TTACAAGCAG AACAGAGCAA AAAGACTTCA GATGCAGCAA
    CAGCAGCTGA AAGAACAGGC AGGACCTGGA TCTCAGAATA AAGTCAGCGC TCTCTTAGAG
    CCCATTCTCC CACTAACCCA CATGCAAGAT CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA
    GAGTGTCAAG CTCAGACCAG ACCCAGCTCC ACCACACTCA ACAATGGACG CACAACTCTT
    GACAACACAA TAAAAGGGTC TTCATCCTGG GCCTCAGAGA AATCCAAGGA AGTCCAAAAG
    TCTAAACGGA GACCAATGTC TAGAGGTCAA TCTGAGGATG CCTCAAATGC AAACACTGCC
    CAGGAGTCAG TAGAAATGAG AAAGATGGAG AACAGTGCCA ACACAGTGAC CTCTGGTGGC
    CTTGAGGGCC AAGGACGTGC AGCGTCCATG CCAAAACTCA ATGCTGAGAT GCAGAGGAGT
    CATTCCCGTC AATCTCCTGG AACATTACTG GAACCCATTC CAGATTCCCC CATGAGGCGC
    TCTGCATCCA CTTTTGCACC ACAGCGCCCT CACGAGGTGA ATCTGAATGA CTACATTCTG
    GAGAAACCTG TTCAGGAACG CCATCACCAC CATCACCATC ATCACCGATG CCATCATCGC
    CGGGACAGAG ACAAGAAACA GAGATCTCTG GACAGGTCGC CTACTGGCCA TCACAAAACT
    ACAGGCACAG CCACTGACTC TGTAGAGGAG AGGGCACATG ACCGGGGTCG TTCCCATGAG
    AGGAAGCACC ATTCATCTTC AGGAGAGAAG CAGCGGTATT ACTCGTGTGA TCGCTATGCC
    AGTCGGGAAC ACTGTTCCAG CAAATCTGCC GTGGCAAGCT GTGCCACCTC ACCCAGCGAG
    ACCCAGGAGG CCAATCTTAA CAAGCAGGGC AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG
    TTGACTTCAG GGGCGAGCAC TCCAAGCCGC GGGCGCAGAC AGCTGCCCCA GACCCCCCTC
    ACTCCTCGTC CCTCCGTGGC CTACAGGACT GCCAACTCTT CCCCTGTGCA ACTTCTGGCC
    ACAACGACCA GCTTGCTTCC CCCGAGTCCC GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC
    AACGCTCTTT TACTCAGCGG CTCTATCTCC AGCTCGCCGG CCCCCGTCAG TCGCATTAAC
    TCAGAACCCT TCTTGGGGCC CCCGGACCAC CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT
    GTCTTCCAAG AGAATCCGAG CCCTCAGATG GGCCGTTCTC CTCAGACTGT AGGGATGCCC
    TTCGTGGCGA TCCCCCCACC TCCCCCACAG CAGTTTCGCA GGGTCCCCAA CGGGTACCAC
    TTCTCCACGG GCCCCAGCGC CAGCAGAGGA CGGGCACAGC AGTACTACCC AGAGGCAGAG
    GTGGAGGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331854.1
    CDS1..7098
    Translation

    Target ORF information:

    RefSeq Version XM_021476179.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACGAGCGTC AGCTGAACTT CAGTTTCCTG AGGCTCTTTA GAGCAGCCCG ACTCATCAAG
    CTCCTCCGGC AGGGATACAC CATTCGCATC CTGCTCTGGA CATTTGTCCA GTCTTTTAAG
    GCACTGCCTT ATGTTTGCCT TCTAATCGCC ATGCTGTTTT TCATCTACGC CATTATTGGG
    ATGCAGGTGT TTGGAAACAT TGATTTGAAT GATGACACGG CAATCAACCG TCACAACAAC
    TTCCGCACTT TCCTCCAAGC TCTTATGCTT TTGTTCAGGA GTGCCACTGG CGAGGCTTGG
    CATGATATCA TGCTGTCGTG TCTGAGCGAG CGCACCTGTG ATCCGAGCTC AGGCACCCTT
    GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT TTTCCTCTGC
    TCATTTCTGA TGTTGAACCT GTTTGTGGCC GTCATTATGG ACAACTTTGA ATATCTGACA
    CGCGACTCCT CCATCCTGGG CCCTCACCAC CTGGATGAGT TTATTCGAGT GTGGGCGGAG
    TATGACCCAG CCGCATGCGG ACGGATTTCC TATAAGGATA TGTACAATTT GTTGCGAATT
    ATCTCGCCCC CCCTTGGCTT AGGGAAGAAC TGCCCTAATA GGGTAGCATA CAAGCGTCTG
    GTGAAAATGA ACATGCCCAT TGCGGATGAC AACAGTGTAC ACTTTACCTC AACGCTGATG
    GCTCTGATCC GTACTGCCCT GGAGATCAAA CTGGCTTCAG GCATGGTGGC TCAGCGTCTG
    TCAGATGCTG AACTGAAGAA GGAGCTCTCT ACAGTTTGGC CAAACCTGTC TCAGAAGACC
    ATGGACCTGC TGGTCACTCC ACATAAACCT AATGAGCTCA CAGTGGGGAA GGTGTATGCA
    GCTCTTATGA TCTTTGACTA TTACAAGCAG AACAGAGCAA AAAGACTTCA GATGCAGCAA
    CAGCAGCTGA AAGAACAGGC AGGACCTGGA TCTCAGAATA AAGTCAGCGC TCTCTTAGAG
    CCCATTCTCC CACTAACCCA CATGCAAGAT CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA
    GAGTGTCAAG CTCAGACCAG ACCCAGCTCC ACCACACTCA ACAATGGACG CACAACTCTT
    GACAACACAA TAAAAGGGTC TTCATCCTGG GCCTCAGAGA AATCCAAGGA AGTCCAAAAG
    TCTAAACGGA GACCAATGTC TAGAGGTCAA TCTGAGGATG CCTCAAATGC AAACACTGCC
    CAGGAGTCAG TAGAAATGAG AAAGATGGAG AACAGTGCCA ACACAGTGAC CTCTGGTGGC
    CTTGAGGGCC AAGGACGTGC AGCGTCCATG CCAAAACTCA ATGCTGAGAT GCAGAGGAGT
    CATTCCCGTC AATCTCCTGG AACATTACTG GAACCCATTC CAGATTCCCC CATGAGGCGC
    TCTGCATCCA CTTTTGCACC ACAGCGCCCT CACGAGGTGA ATCTGAATGA CTACATTCTG
    GAGAAACCTG TTCAGGAACG CCATCACCAC CATCACCATC ATCACCGATG CCATCATCGC
    CGGGACAGAG ACAAGAAACA GAGATCTCTG GACAGGTCGC CTACTGGCCA TCACAAAACT
    ACAGGCACAG CCACTGACTC TGTAGAGGAG AGGGCACATG ACCGGGGTCG TTCCCATGAG
    AGGAAGCACC ATTCATCTTC AGGAGAGAAG CAGCGGTATT ACTCGTGTGA TCGCTATGCC
    AGTCGGGAAC ACTGTTCCAG CAAATCTGCC GTGGCAAGCT GTGCCACCTC ACCCAGCGAG
    ACCCAGGAGG CCAATCTTAA CAAGCAGGGC AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG
    TTGACTTCAG GGGCGAGCAC TCCAAGCCGC GGGCGCAGAC AGCTGCCCCA GACCCCCCTC
    ACTCCTCGTC CCTCCGTGGC CTACAGGACT GCCAACTCTT CCCCTGTGCA ACTTCTGGCC
    ACAACGACCA GCTTGCTTCC CCCGAGTCCC GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC
    AACGCTCTTT TACTCAGCGG CTCTATCTCC AGCTCGCCGG CCCCCGTCAG TCGCATTAAC
    TCAGAACCCT TCTTGGGGCC CCCGGACCAC CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT
    GTCTTCCAAG AGAATCCGAG CCCTCAGATG GGCCGTTCTC CTCAGACTGT AGGGATGCCC
    TTCGTGGCGA TCCCCCCACC TCCCCCACAG CAGTTTCGCA GGGTCCCCAA CGGGTACCAC
    TTCTCCACGG GCCCCAGCGC CAGCAGAGGA CGGGCACAGC AGTACTACCC AGAGGCAGAG
    GTGGAGGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142645
    Clone ID Related Accession (Same CDS sequence) XM_021476180.1
    Accession Version XM_021476180.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC
    AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC
    ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT
    CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT
    GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT
    CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT
    CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG
    TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC
    CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC
    CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG
    GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT
    GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG
    GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG
    GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA
    CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT
    TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA
    GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC
    ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA
    CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT
    TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT
    AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA
    AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA
    GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA
    ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA
    CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC
    CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG
    AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT
    GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA
    GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC
    AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC
    AAGCAGTGGG TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331855.1
    CDS1..6618
    Translation

    Target ORF information:

    RefSeq Version XM_021476180.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476180.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC
    AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC
    ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT
    CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT
    GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT
    CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT
    CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG
    TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC
    CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC
    CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG
    GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT
    GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG
    GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG
    GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA
    CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT
    TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA
    GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC
    ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA
    CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT
    TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT
    AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA
    AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA
    GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA
    ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA
    CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC
    CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG
    AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT
    GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA
    GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC
    AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC
    AAGCAGTGGG TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142646
    Clone ID Related Accession (Same CDS sequence) XM_021476181.1
    Accession Version XM_021476181.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG
    CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG
    AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC
    GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG
    GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA
    CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC
    ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT
    GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC
    GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC
    ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG
    AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC
    CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC
    ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT
    GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG
    GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT
    GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC
    ACAACGGAGC GTCAGCTGAA CTTCAGTTTC CTGAGGCTCT TTAGAGCAGC CCGACTCATC
    AAGCTCCTCC GGCAGGGATA CACCATTCGC ATCCTGCTCT GGACATTTGT CCAGTCTTTT
    AAGGCACTGC CTTATGTTTG CCTTCTAATC GCCATGCTGT TTTTCATCTA CGCCATTATT
    GGGATGCAGG TGTTTGGAAA CATTGATTTG AATGATGACA CGGCAATCAA CCGTCACAAC
    AACTTCCGCA CTTTCCTCCA AGCTCTTATG CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT
    TGGCATGATA TCATGCTGTC GTGTCTGAGC GAGCGCACCT GTGATCCGAG CTCAGGCACC
    CTTGGAAAGG AGTGTGGGAG TGACTTTGCC TACTTCTACT TTGTCTCCTT CATTTTCCTC
    TGCTCATTTC TGATGTTGAA CCTGTTTGTG GCCGTCATTA TGGACAACTT TGAATATCTG
    ACACGCGACT CCTCCATCCT GGGCCCTCAC CACCTGGATG AGTTTATTCG AGTGTGGGCG
    GAGTATGACC CAGCCGCATG CGGACGGATT TCCTATAAGG ATATGTACAA TTTGTTGCGA
    ATTATCTCGC CCCCCCTTGG CTTAGGGAAG AACTGCCCTA ATAGGGTAGC ATACAAGCGT
    CTGGTGAAAA TGAACATGCC CATTGCGGAT GACAACAGTG TACACTTTAC CTCAACGCTG
    ATGGCTCTGA TCCGTACTGC CCTGGAGATC AAACTGGCTT CAGGCATGGT GGCTCAGCGT
    CTGTCAGATG CTGAACTGAA GAAGGAGCTC TCTACAGTTT GGCCAAACCT GTCTCAGAAG
    ACCATGGACC TGCTGGTCAC TCCACATAAA CCTAATGAGC TCACAGTGGG GAAGGTGTAT
    GCAGCTCTTA TGATCTTTGA CTATTACAAG CAGAACAGAG CAAAAAGACT TCAGATGCAG
    CAACAGCAGC TGAAAGAACA GGCAGGACCT GGATCTCAGA ATAAAGTCAG CGCTCTCTTA
    GAGCCCATTC TCCCACTAAC CCACATGCAA GATCAGCCAG TGAACGGTGT CGATTCAGCT
    TCAGAGTGTC AAGCTCAGAC CAGACCCAGC TCCACCACAC TCAACAATGG ACGCACAACT
    CTTGACAACA CAATAAAAGG GTCTTCATCC TGGGCCTCAG AGAAATCCAA GGAAGTCCAA
    AAGTCTAAAC GGAGACCAAT GTCTAGAGGT CAATCTGAGG ATGCCTCAAA TGCAAACACT
    GCCCAGGAGT CAGTAGAAAT GAGAAAGATG GAGAACAGTG CCAACACAGT GACCTCTGGT
    GGCCTTGAGG GCCAAGGACG TGCAGCGTCC ATGCCAAAAC TCAATGCTGA GATGCAGAGG
    AGTCATTCCC GTCAATCTCC TGGAACATTA CTGGAACCCA TTCCAGATTC CCCCATGAGG
    CGCTCTGCAT CCACTTTTGC ACCACAGCGC CCTCACGAGG TGAATCTGAA TGACTACATT
    CTGGAGAAAC CTGTTCAGGA ACGCCATCAC CACCATCACC ATCATCACCG ATGCCATCAT
    CGCCGGGACA GAGACAAGAA ACAGAGATCT CTGGACAGGT CGCCTACTGG CCATCACAAA
    ACTACAGGCA CAGCCACTGA CTCTGTAGAG GAGAGGGCAC ATGACCGGGG TCGTTCCCAT
    GAGAGGAAGC ACCATTCATC TTCAGGAGAG AAGCAGCGGT ATTACTCGTG TGATCGCTAT
    GCCAGTCGGG AACACTGTTC CAGCAAATCT GCCGTGGCAA GCTGTGCCAC CTCACCCAGC
    GAGACCCAGG AGGCCAATCT TAACAAGCAG GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG
    TTGTTGACTT CAGGGGCGAG CACTCCAAGC CGCGGGCGCA GACAGCTGCC CCAGACCCCC
    CTCACTCCTC GTCCCTCCGT GGCCTACAGG ACTGCCAACT CTTCCCCTGT GCAACTTCTG
    GCCACAACGA CCAGCTTGCT TCCCCCGAGT CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG
    CACAACGCTC TTTTACTCAG CGGCTCTATC TCCAGCTCGC CGGCCCCCGT CAGTCGCATT
    AACTCAGAAC CCTTCTTGGG GCCCCCGGAC CACCCTGCTG GCTTGCAGCC CGAAGCCTTC
    GCTGTCTTCC AAGAGAATCC GAGCCCTCAG ATGGGCCGTT CTCCTCAGAC TGTAGGGATG
    CCCTTCGTGG CGATCCCCCC ACCTCCCCCA CAGCAGTTTC GCAGGGTCCC CAACGGGTAC
    CACTTCTCCA CGGGCCCCAG CGCCAGCAGA GGACGGGCAC AGCAGTACTA CCCAGAGGCA
    GAGGTGGAGG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331856.1
    CDS637..7677
    Translation

    Target ORF information:

    RefSeq Version XM_021476181.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476181.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG
    CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG
    AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC
    GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG
    GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA
    CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC
    ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT
    GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC
    GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC
    ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG
    AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC
    CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC
    ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT
    GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG
    GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT
    GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC
    ACAACGGAGC GTCAGCTGAA CTTCAGTTTC CTGAGGCTCT TTAGAGCAGC CCGACTCATC
    AAGCTCCTCC GGCAGGGATA CACCATTCGC ATCCTGCTCT GGACATTTGT CCAGTCTTTT
    AAGGCACTGC CTTATGTTTG CCTTCTAATC GCCATGCTGT TTTTCATCTA CGCCATTATT
    GGGATGCAGG TGTTTGGAAA CATTGATTTG AATGATGACA CGGCAATCAA CCGTCACAAC
    AACTTCCGCA CTTTCCTCCA AGCTCTTATG CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT
    TGGCATGATA TCATGCTGTC GTGTCTGAGC GAGCGCACCT GTGATCCGAG CTCAGGCACC
    CTTGGAAAGG AGTGTGGGAG TGACTTTGCC TACTTCTACT TTGTCTCCTT CATTTTCCTC
    TGCTCATTTC TGATGTTGAA CCTGTTTGTG GCCGTCATTA TGGACAACTT TGAATATCTG
    ACACGCGACT CCTCCATCCT GGGCCCTCAC CACCTGGATG AGTTTATTCG AGTGTGGGCG
    GAGTATGACC CAGCCGCATG CGGACGGATT TCCTATAAGG ATATGTACAA TTTGTTGCGA
    ATTATCTCGC CCCCCCTTGG CTTAGGGAAG AACTGCCCTA ATAGGGTAGC ATACAAGCGT
    CTGGTGAAAA TGAACATGCC CATTGCGGAT GACAACAGTG TACACTTTAC CTCAACGCTG
    ATGGCTCTGA TCCGTACTGC CCTGGAGATC AAACTGGCTT CAGGCATGGT GGCTCAGCGT
    CTGTCAGATG CTGAACTGAA GAAGGAGCTC TCTACAGTTT GGCCAAACCT GTCTCAGAAG
    ACCATGGACC TGCTGGTCAC TCCACATAAA CCTAATGAGC TCACAGTGGG GAAGGTGTAT
    GCAGCTCTTA TGATCTTTGA CTATTACAAG CAGAACAGAG CAAAAAGACT TCAGATGCAG
    CAACAGCAGC TGAAAGAACA GGCAGGACCT GGATCTCAGA ATAAAGTCAG CGCTCTCTTA
    GAGCCCATTC TCCCACTAAC CCACATGCAA GATCAGCCAG TGAACGGTGT CGATTCAGCT
    TCAGAGTGTC AAGCTCAGAC CAGACCCAGC TCCACCACAC TCAACAATGG ACGCACAACT
    CTTGACAACA CAATAAAAGG GTCTTCATCC TGGGCCTCAG AGAAATCCAA GGAAGTCCAA
    AAGTCTAAAC GGAGACCAAT GTCTAGAGGT CAATCTGAGG ATGCCTCAAA TGCAAACACT
    GCCCAGGAGT CAGTAGAAAT GAGAAAGATG GAGAACAGTG CCAACACAGT GACCTCTGGT
    GGCCTTGAGG GCCAAGGACG TGCAGCGTCC ATGCCAAAAC TCAATGCTGA GATGCAGAGG
    AGTCATTCCC GTCAATCTCC TGGAACATTA CTGGAACCCA TTCCAGATTC CCCCATGAGG
    CGCTCTGCAT CCACTTTTGC ACCACAGCGC CCTCACGAGG TGAATCTGAA TGACTACATT
    CTGGAGAAAC CTGTTCAGGA ACGCCATCAC CACCATCACC ATCATCACCG ATGCCATCAT
    CGCCGGGACA GAGACAAGAA ACAGAGATCT CTGGACAGGT CGCCTACTGG CCATCACAAA
    ACTACAGGCA CAGCCACTGA CTCTGTAGAG GAGAGGGCAC ATGACCGGGG TCGTTCCCAT
    GAGAGGAAGC ACCATTCATC TTCAGGAGAG AAGCAGCGGT ATTACTCGTG TGATCGCTAT
    GCCAGTCGGG AACACTGTTC CAGCAAATCT GCCGTGGCAA GCTGTGCCAC CTCACCCAGC
    GAGACCCAGG AGGCCAATCT TAACAAGCAG GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG
    TTGTTGACTT CAGGGGCGAG CACTCCAAGC CGCGGGCGCA GACAGCTGCC CCAGACCCCC
    CTCACTCCTC GTCCCTCCGT GGCCTACAGG ACTGCCAACT CTTCCCCTGT GCAACTTCTG
    GCCACAACGA CCAGCTTGCT TCCCCCGAGT CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG
    CACAACGCTC TTTTACTCAG CGGCTCTATC TCCAGCTCGC CGGCCCCCGT CAGTCGCATT
    AACTCAGAAC CCTTCTTGGG GCCCCCGGAC CACCCTGCTG GCTTGCAGCC CGAAGCCTTC
    GCTGTCTTCC AAGAGAATCC GAGCCCTCAG ATGGGCCGTT CTCCTCAGAC TGTAGGGATG
    CCCTTCGTGG CGATCCCCCC ACCTCCCCCA CAGCAGTTTC GCAGGGTCCC CAACGGGTAC
    CACTTCTCCA CGGGCCCCAG CGCCAGCAGA GGACGGGCAC AGCAGTACTA CCCAGAGGCA
    GAGGTGGAGG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142647
    Clone ID Related Accession (Same CDS sequence) XM_021476182.1
    Accession Version XM_021476182.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG
    CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG
    AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC
    GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG
    GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA
    CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC
    ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT
    GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC
    GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC
    ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG
    AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC
    CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC
    ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT
    GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG
    GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT
    GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC
    GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC
    CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA
    CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG
    CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC
    CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT
    GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA
    AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA
    TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC
    GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT
    GACCCAGCCG CATGCGGACG GATTTCCTAT AAGGATATGT ACAATTTGTT GCGAATTATC
    TCGCCCCCCC TTGGCTTAGG GAAGAACTGC CCTAATAGGG TAGCATACAA GCGTCTGGTG
    AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT
    CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA
    GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG
    GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT
    CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG
    CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC
    ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG
    TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC
    AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT
    AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG
    GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT
    GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT
    TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT
    GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG
    AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG
    GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA
    GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG
    AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT
    CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC
    CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG
    ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT
    CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA
    ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC
    GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA
    GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC
    TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC
    GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC
    TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG
    GAGGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331857.1
    CDS637..7671
    Translation

    Target ORF information:

    RefSeq Version XM_021476182.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG
    CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG
    AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC
    GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG
    GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA
    CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC
    ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT
    GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC
    GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC
    ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG
    AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC
    CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC
    ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT
    GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG
    GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT
    GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC
    GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC
    CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA
    CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG
    CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC
    CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT
    GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA
    AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA
    TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC
    GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT
    GACCCAGCCG CATGCGGACG GATTTCCTAT AAGGATATGT ACAATTTGTT GCGAATTATC
    TCGCCCCCCC TTGGCTTAGG GAAGAACTGC CCTAATAGGG TAGCATACAA GCGTCTGGTG
    AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT
    CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA
    GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG
    GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT
    CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG
    CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC
    ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG
    TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC
    AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT
    AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG
    GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT
    GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT
    TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT
    GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG
    AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG
    GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA
    GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG
    AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT
    CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC
    CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG
    ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT
    CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA
    ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC
    GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA
    GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC
    TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC
    GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC
    TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG
    GAGGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59085
    Clone ID Related Accession (Same CDS sequence) XM_017356095.1 , XM_017356095.2
    Accession Version XM_017356095.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CGAGTTATGT CTGCTCCCCT
    GCACATCACT ACTTGAGTAA AGAACAGCAG CGTTCTCTAA AAATGATGTC CGTGTGGGAG
    CAGCGCACCA CTCAGATCCG AAAGCACATG CTAGCCAGCA GTGAGGCGTT GTACCAAGAA
    GAGCTCAATC CACGGCTCAT GTCTAACCTT CCTCTACGCA CCGACATAAA AACCCACCTG
    GACCGGCCTT TGGTTGTGGA GCCTCGCTGT GATGGCCCCA TCAGCCCACA CAGTGGCTGG
    GACAAACCCC AGGATTTGCA AGGCGAGGGC AGCACACCAG AAGAACGAGT CCCACCTGTA
    TTCGAGCCTC CACAGTCACA TCCACCACGC AAGCACCACC GTCACAGAGA GCGTCTTTCT
    AATGAGACCA ATGAAAATGG AGACACGGGT CATGCCAAAG AAGGCCGGCA TCACGTTCAT
    CACAGCCGAA GTAAAGAGCA TGATGGGACA CGTTGTAAGG AGGGGAAGGG TGACAGGAGC
    CGAAGCAGAG AAGGGGGACG TAGACATCAC CATCAGAGCT CGGTGGATGA GGGCGTAGGA
    GGTGGAGAGA GGGAACATCG GCACCATCAC TCCCATAGGC ATAACAGAGA GGGCAATGGT
    GTGGTCAGTG CTGGGGGGAA GAGTGAACGG CGGTCCAGGC AGAAAGAGGG CTCACGATCA
    GGGACAAGAG AGGGAGAGAG ATGCTCAAGG GGGGAGAATG GAGGAAATGG AGGGAGGAGG
    CGACACAGGC CACGAACCAG TAAAGCTCAG TCTACGCTGG AAGGAGAGGA GCAACATGTG
    AATGGAGAGG AGGAGGGCAA GTCGCAAAGC CATAGGCACA TGGATATGGG AGGAGATTCA
    ATGGCTGTAA ACCCCCATCA ACCTTCAGTT GGAAGCCGGT GGTGTCTTGA GAAACCAGAA
    GATTCGGACA ACAAACGAAA TATTGGTCAG ACTGGAGGAA TGAGCACCCA TATACCCGTG
    ACTGTCACTT CTCCCCCGGG AGAAACAACC CTTATTCCCA TGAACAGTAT AGACAGCGAG
    ACAGTGCCCA TGACTGAGAA GAATCTAGAA GATCTAAATC AAAGCAGCAT TCGTCCCATC
    CTCCCCTTCA GCTCCATGTT CATTTTCAAC CCAACTAATC CGGTGAGACG TTTATGCCAC
    TACATTGTGA GTCTGCGCTA TTTTGAGATG TGTATCCTGG TGGTCATTGC CATGAGCAGT
    ATAGCCCTGG CTGCAGAAGA CCCGGTCCAA GCCAATGCAC CTCGGAACAA TGTCCTGAAG
    TATTTGGACT ATGTCTTCAC TGGTGTTTTC ACATTCGAGA TGGTCATTAA GATGGTGGAT
    TTAGGTTTAA TTCTGCATCC TGGTTCCTAC TTTCGAGATC TATGGAATAT TTTGGACTTC
    ATTGTGGTCA GTGGTGCACT GGTGGCATTC GCATTCTCGA GCTTCATGGG ATCACATCAA
    AGCGTAAACA GAGGAACCAA GGGCAAAGAC ATCAGCACTA TCAAGTCACT GAGGGTGTTG
    CGAGTCTTGC GACCTCTGAA GACCATCAAA CGTCTACCAA AACTGAAGGC CGTGTTTGAT
    TGTGTGGTGA ACTCCCTGAA GAATGTCCTG AATATCCTCA TTGTCTACAT TCTGTTCATG
    TTCATTTTCG CTGTCATTGC TGTGCAGCTC TTCAAGGGCA AGTTTTTTTA CTGCACAGAC
    GAGTCCAAGG GCCTGGAGAA AGACTGCAGG GGGCTGTTTT TGGATTATGA TAAGGATGAT
    GTGACGGCAC AACCGAGGGA ATGGAAGAAA TACGAGTTTC ACTATGACAA TGTGCTTTGG
    GCTTTTCTCA CTCTCTTCAC TGTTTCTACG GGAGAGGGCT GGCCTACGGT TTTGAAGCAC
    TCCGTGGATG CCACTTTTGA GGATCAGGGT CCCAGTCCAG GCTACCGCAT TGAGATGTCT
    ATTTTTTACG TGGTCTACTT TGTGGTTTTC CCTTTCTTCT TCGTCAACAT TTTTGTAGCC
    TTGATAATCA TCACATTCCA GGAACAGGGT GATAAGGTCT TGTCAGAGTG CAGTCTGGAG
    AAGAATGAGA GAGCCTGCAT CGACTTTGCT ATCAATGCCA AACCTCTGAC ACGCTACATG
    CCGCAGAACC AGCAGTCCTT TCAGTACCGC CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT
    GAGTACTCCA TCATGATCAT GATTGCTCTC AACACTGTGG TGCTCATGAT GAAGTTCCAT
    GGGGCGCCTG ACTTTTATGA GGCAATGCTT AAGTATCTCA ACATTGTCTT CACTGTTCTC
    TTTTCACTGG AGTGTATTCT CAAGATTATT GCTTTCGGCC CCTTGAATTA CCTGAAAGAT
    GCCTGGAATG TGTTTGACTT TGTGACTGTG CTGGGCAGCA TCACTGACAT TTTAGTCACT
    GAGATTAACA CAACGGAGCG TCAGCTGAAC TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC
    CGACTCATCA AGCTCCTCCG GCAGGGATAC ACCATTCGCA TCCTGCTCTG GACATTTGTC
    CAGTCTTTTA AGGCACTGCC TTATGTTTGC CTTCTAATCG CCATGCTGTT TTTCATCTAC
    GCCATTATTG GGATGCAGGT GTTTGGAAAC ATTGATTTGA ATGATGACAC GGCAATCAAC
    CGTCACAACA ACTTCCGCAC TTTCCTCCAA GCTCTTATGC TTTTGTTCAG GAGTGCCACT
    GGCGAGGCTT GGCATGATAT CATGCTGTCG TGTCTGAGCG AGCGCACCTG TGATCCGAGC
    TCAGGCACCC TTGGAAAGGA GTGTGGGAGT GACTTTGCCT ACTTCTACTT TGTCTCCTTC
    ATTTTCCTCT GCTCATTTCT GATGTTGAAC CTGTTTGTGG CCGTCATTAT GGACAACTTT
    GAATATCTGA CACGCGACTC CTCCATCCTG GGCCCTCACC ACCTGGATGA GTTTATTCGA
    GTGTGGGCGG AGTATGACCC AGCCGCATGT GGCCGTATCA AGTACCTCGA TATGTATGAG
    ATGCTGCTCC ACATGTCCCC ACCTTTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC
    TACAAGCGTC TGGTGAAAAT GAACATGCCC ATTGCGGATG ACAACAGTGT ACACTTTACC
    TCAACGCTGA TGGCTCTGAT CCGTACTGCC CTGGAGATCA AACTGGCTTC AGGCATGGTG
    GCTCAGCGTC TGTCAGATGC TGAACTGAAG AAGGAGCTCT CTACAGTTTG GCCAAACCTG
    TCTCAGAAGA CCATGGACCT GCTGGTCACT CCACATAAAC CTAATGAGCT CACAGTGGGG
    AAGGTGTATG CAGCTCTTAT GATCTTTGAC TATTACAAGC AGAACAGAGC AAAAAGACTT
    CAGATGCAGC AACAGCAGCT GAAAGAACAG GCAGGACCTG GATCTCAGAA TAAAGTCAGC
    GCTCTCTTAG AGCCCATTCT CCCACTAACC CACATGCAAG ATCAGCCAGT GAACGGTGTC
    GATTCAGCTT CAGAGTGTCA AGCTCAGACC AGACCCAGCT CCACCACACT CAACAATGGA
    CGCACAACTC TTGACAACAC AATAAAAGGG TCTTCATCCT GGGCCTCAGA GAAATCCAAG
    GAAGTCCAAA AGTCTAAACG GAGACCAATG TCTAGAGGTC AATCTGAGGA TGCCTCAAAT
    GCAAACACTG CCCAGGAGTC AGTAGAAATG AGAAAGATGG AGAACAGTGC CAACACAGTG
    ACCTCTGGTG GCCTTGAGGG CCAAGGACGT GCAGCGTCCA TGCCAAAACT CAATGCTGAG
    ATGCAGAGGA GTCATTCCCG TCAATCTCCT GGAACATTAC TGGAACCCAT TCCAGATTCC
    CCCATGAGGC GCTCTGCATC CACTTTTGCA CCACAGCGCC CTCACGAGGT GAATCTGAAT
    GACTACATTC TGGAGAAACC TGTTCAGGAA CGCCATCACC ACCATCACCA TCATCACCGA
    TGCCATCATC GCCGGGACAG AGACAAGAAA CAGAGATCTC TGGACAGGTC GCCTACTGGC
    CATCACAAAA CTACAGGCAC AGCCACTGAC TCTGTAGAGG AGAGGGCACA TGACCGGGGT
    CGTTCCCATG AGAGGAAGCA CCATTCATCT TCAGGAGAGA AGCAGCGGTA TTACTCGTGT
    GATCGCTATG CCAGTCGGGA ACACTGTTCC AGCAAATCTG CCGTGGCAAG CTGTGCCACC
    TCACCCAGCG AGACCCAGGA GGCCAATCTT AACAAGCAGG GCAGTGGGTG GGCTAAGGGG
    AGCCCGGTGT TGTTGACTTC AGGGGCGAGC ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC
    CAGACCCCCC TCACTCCTCG TCCCTCCGTG GCCTACAGGA CTGCCAACTC TTCCCCTGTG
    CAACTTCTGG CCACAACGAC CAGCTTGCTT CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT
    CTGTCCGAGC ACAACGCTCT TTTACTCAGC GGCTCTATCT CCAGCTCGCC GGCCCCCGTC
    AGTCGCATTA ACTCAGAACC CTTCTTGGGG CCCCCGGACC ACCCTGCTGG CTTGCAGCCC
    GAAGCCTTCG CTGTCTTCCA AGAGAATCCG AGCCCTCAGA TGGGCCGTTC TCCTCAGACT
    GTAGGGATGC CCTTCGTGGC GATCCCCCCA CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC
    AACGGGTACC ACTTCTCCAC GGGCCCCAGC GCCAGCAGAG GACGGGCACA GCAGTACTAC
    CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211584.1
    CDS636..7745
    Translation

    Target ORF information:

    RefSeq Version XM_017356095.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356095.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CGAGTTATGT CTGCTCCCCT
    GCACATCACT ACTTGAGTAA AGAACAGCAG CGTTCTCTAA AAATGATGTC CGTGTGGGAG
    CAGCGCACCA CTCAGATCCG AAAGCACATG CTAGCCAGCA GTGAGGCGTT GTACCAAGAA
    GAGCTCAATC CACGGCTCAT GTCTAACCTT CCTCTACGCA CCGACATAAA AACCCACCTG
    GACCGGCCTT TGGTTGTGGA GCCTCGCTGT GATGGCCCCA TCAGCCCACA CAGTGGCTGG
    GACAAACCCC AGGATTTGCA AGGCGAGGGC AGCACACCAG AAGAACGAGT CCCACCTGTA
    TTCGAGCCTC CACAGTCACA TCCACCACGC AAGCACCACC GTCACAGAGA GCGTCTTTCT
    AATGAGACCA ATGAAAATGG AGACACGGGT CATGCCAAAG AAGGCCGGCA TCACGTTCAT
    CACAGCCGAA GTAAAGAGCA TGATGGGACA CGTTGTAAGG AGGGGAAGGG TGACAGGAGC
    CGAAGCAGAG AAGGGGGACG TAGACATCAC CATCAGAGCT CGGTGGATGA GGGCGTAGGA
    GGTGGAGAGA GGGAACATCG GCACCATCAC TCCCATAGGC ATAACAGAGA GGGCAATGGT
    GTGGTCAGTG CTGGGGGGAA GAGTGAACGG CGGTCCAGGC AGAAAGAGGG CTCACGATCA
    GGGACAAGAG AGGGAGAGAG ATGCTCAAGG GGGGAGAATG GAGGAAATGG AGGGAGGAGG
    CGACACAGGC CACGAACCAG TAAAGCTCAG TCTACGCTGG AAGGAGAGGA GCAACATGTG
    AATGGAGAGG AGGAGGGCAA GTCGCAAAGC CATAGGCACA TGGATATGGG AGGAGATTCA
    ATGGCTGTAA ACCCCCATCA ACCTTCAGTT GGAAGCCGGT GGTGTCTTGA GAAACCAGAA
    GATTCGGACA ACAAACGAAA TATTGGTCAG ACTGGAGGAA TGAGCACCCA TATACCCGTG
    ACTGTCACTT CTCCCCCGGG AGAAACAACC CTTATTCCCA TGAACAGTAT AGACAGCGAG
    ACAGTGCCCA TGACTGAGAA GAATCTAGAA GATCTAAATC AAAGCAGCAT TCGTCCCATC
    CTCCCCTTCA GCTCCATGTT CATTTTCAAC CCAACTAATC CGGTGAGACG TTTATGCCAC
    TACATTGTGA GTCTGCGCTA TTTTGAGATG TGTATCCTGG TGGTCATTGC CATGAGCAGT
    ATAGCCCTGG CTGCAGAAGA CCCGGTCCAA GCCAATGCAC CTCGGAACAA TGTCCTGAAG
    TATTTGGACT ATGTCTTCAC TGGTGTTTTC ACATTCGAGA TGGTCATTAA GATGGTGGAT
    TTAGGTTTAA TTCTGCATCC TGGTTCCTAC TTTCGAGATC TATGGAATAT TTTGGACTTC
    ATTGTGGTCA GTGGTGCACT GGTGGCATTC GCATTCTCGA GCTTCATGGG ATCACATCAA
    AGCGTAAACA GAGGAACCAA GGGCAAAGAC ATCAGCACTA TCAAGTCACT GAGGGTGTTG
    CGAGTCTTGC GACCTCTGAA GACCATCAAA CGTCTACCAA AACTGAAGGC CGTGTTTGAT
    TGTGTGGTGA ACTCCCTGAA GAATGTCCTG AATATCCTCA TTGTCTACAT TCTGTTCATG
    TTCATTTTCG CTGTCATTGC TGTGCAGCTC TTCAAGGGCA AGTTTTTTTA CTGCACAGAC
    GAGTCCAAGG GCCTGGAGAA AGACTGCAGG GGGCTGTTTT TGGATTATGA TAAGGATGAT
    GTGACGGCAC AACCGAGGGA ATGGAAGAAA TACGAGTTTC ACTATGACAA TGTGCTTTGG
    GCTTTTCTCA CTCTCTTCAC TGTTTCTACG GGAGAGGGCT GGCCTACGGT TTTGAAGCAC
    TCCGTGGATG CCACTTTTGA GGATCAGGGT CCCAGTCCAG GCTACCGCAT TGAGATGTCT
    ATTTTTTACG TGGTCTACTT TGTGGTTTTC CCTTTCTTCT TCGTCAACAT TTTTGTAGCC
    TTGATAATCA TCACATTCCA GGAACAGGGT GATAAGGTCT TGTCAGAGTG CAGTCTGGAG
    AAGAATGAGA GAGCCTGCAT CGACTTTGCT ATCAATGCCA AACCTCTGAC ACGCTACATG
    CCGCAGAACC AGCAGTCCTT TCAGTACCGC CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT
    GAGTACTCCA TCATGATCAT GATTGCTCTC AACACTGTGG TGCTCATGAT GAAGTTCCAT
    GGGGCGCCTG ACTTTTATGA GGCAATGCTT AAGTATCTCA ACATTGTCTT CACTGTTCTC
    TTTTCACTGG AGTGTATTCT CAAGATTATT GCTTTCGGCC CCTTGAATTA CCTGAAAGAT
    GCCTGGAATG TGTTTGACTT TGTGACTGTG CTGGGCAGCA TCACTGACAT TTTAGTCACT
    GAGATTAACA CAACGGAGCG TCAGCTGAAC TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC
    CGACTCATCA AGCTCCTCCG GCAGGGATAC ACCATTCGCA TCCTGCTCTG GACATTTGTC
    CAGTCTTTTA AGGCACTGCC TTATGTTTGC CTTCTAATCG CCATGCTGTT TTTCATCTAC
    GCCATTATTG GGATGCAGGT GTTTGGAAAC ATTGATTTGA ATGATGACAC GGCAATCAAC
    CGTCACAACA ACTTCCGCAC TTTCCTCCAA GCTCTTATGC TTTTGTTCAG GAGTGCCACT
    GGCGAGGCTT GGCATGATAT CATGCTGTCG TGTCTGAGCG AGCGCACCTG TGATCCGAGC
    TCAGGCACCC TTGGAAAGGA GTGTGGGAGT GACTTTGCCT ACTTCTACTT TGTCTCCTTC
    ATTTTCCTCT GCTCATTTCT GATGTTGAAC CTGTTTGTGG CCGTCATTAT GGACAACTTT
    GAATATCTGA CACGCGACTC CTCCATCCTG GGCCCTCACC ACCTGGATGA GTTTATTCGA
    GTGTGGGCGG AGTATGACCC AGCCGCATGT GGCCGTATCA AGTACCTCGA TATGTATGAG
    ATGCTGCTCC ACATGTCCCC ACCTTTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC
    TACAAGCGTC TGGTGAAAAT GAACATGCCC ATTGCGGATG ACAACAGTGT ACACTTTACC
    TCAACGCTGA TGGCTCTGAT CCGTACTGCC CTGGAGATCA AACTGGCTTC AGGCATGGTG
    GCTCAGCGTC TGTCAGATGC TGAACTGAAG AAGGAGCTCT CTACAGTTTG GCCAAACCTG
    TCTCAGAAGA CCATGGACCT GCTGGTCACT CCACATAAAC CTAATGAGCT CACAGTGGGG
    AAGGTGTATG CAGCTCTTAT GATCTTTGAC TATTACAAGC AGAACAGAGC AAAAAGACTT
    CAGATGCAGC AACAGCAGCT GAAAGAACAG GCAGGACCTG GATCTCAGAA TAAAGTCAGC
    GCTCTCTTAG AGCCCATTCT CCCACTAACC CACATGCAAG ATCAGCCAGT GAACGGTGTC
    GATTCAGCTT CAGAGTGTCA AGCTCAGACC AGACCCAGCT CCACCACACT CAACAATGGA
    CGCACAACTC TTGACAACAC AATAAAAGGG TCTTCATCCT GGGCCTCAGA GAAATCCAAG
    GAAGTCCAAA AGTCTAAACG GAGACCAATG TCTAGAGGTC AATCTGAGGA TGCCTCAAAT
    GCAAACACTG CCCAGGAGTC AGTAGAAATG AGAAAGATGG AGAACAGTGC CAACACAGTG
    ACCTCTGGTG GCCTTGAGGG CCAAGGACGT GCAGCGTCCA TGCCAAAACT CAATGCTGAG
    ATGCAGAGGA GTCATTCCCG TCAATCTCCT GGAACATTAC TGGAACCCAT TCCAGATTCC
    CCCATGAGGC GCTCTGCATC CACTTTTGCA CCACAGCGCC CTCACGAGGT GAATCTGAAT
    GACTACATTC TGGAGAAACC TGTTCAGGAA CGCCATCACC ACCATCACCA TCATCACCGA
    TGCCATCATC GCCGGGACAG AGACAAGAAA CAGAGATCTC TGGACAGGTC GCCTACTGGC
    CATCACAAAA CTACAGGCAC AGCCACTGAC TCTGTAGAGG AGAGGGCACA TGACCGGGGT
    CGTTCCCATG AGAGGAAGCA CCATTCATCT TCAGGAGAGA AGCAGCGGTA TTACTCGTGT
    GATCGCTATG CCAGTCGGGA ACACTGTTCC AGCAAATCTG CCGTGGCAAG CTGTGCCACC
    TCACCCAGCG AGACCCAGGA GGCCAATCTT AACAAGCAGG GCAGTGGGTG GGCTAAGGGG
    AGCCCGGTGT TGTTGACTTC AGGGGCGAGC ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC
    CAGACCCCCC TCACTCCTCG TCCCTCCGTG GCCTACAGGA CTGCCAACTC TTCCCCTGTG
    CAACTTCTGG CCACAACGAC CAGCTTGCTT CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT
    CTGTCCGAGC ACAACGCTCT TTTACTCAGC GGCTCTATCT CCAGCTCGCC GGCCCCCGTC
    AGTCGCATTA ACTCAGAACC CTTCTTGGGG CCCCCGGACC ACCCTGCTGG CTTGCAGCCC
    GAAGCCTTCG CTGTCTTCCA AGAGAATCCG AGCCCTCAGA TGGGCCGTTC TCCTCAGACT
    GTAGGGATGC CCTTCGTGGC GATCCCCCCA CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC
    AACGGGTACC ACTTCTCCAC GGGCCCCAGC GCCAGCAGAG GACGGGCACA GCAGTACTAC
    CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59085
    Clone ID Related Accession (Same CDS sequence) XM_017356095.1 , XM_017356095.2
    Accession Version XM_017356095.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CGAGTTATGT CTGCTCCCCT
    GCACATCACT ACTTGAGTAA AGAACAGCAG CGTTCTCTAA AAATGATGTC CGTGTGGGAG
    CAGCGCACCA CTCAGATCCG AAAGCACATG CTAGCCAGCA GTGAGGCGTT GTACCAAGAA
    GAGCTCAATC CACGGCTCAT GTCTAACCTT CCTCTACGCA CCGACATAAA AACCCACCTG
    GACCGGCCTT TGGTTGTGGA GCCTCGCTGT GATGGCCCCA TCAGCCCACA CAGTGGCTGG
    GACAAACCCC AGGATTTGCA AGGCGAGGGC AGCACACCAG AAGAACGAGT CCCACCTGTA
    TTCGAGCCTC CACAGTCACA TCCACCACGC AAGCACCACC GTCACAGAGA GCGTCTTTCT
    AATGAGACCA ATGAAAATGG AGACACGGGT CATGCCAAAG AAGGCCGGCA TCACGTTCAT
    CACAGCCGAA GTAAAGAGCA TGATGGGACA CGTTGTAAGG AGGGGAAGGG TGACAGGAGC
    CGAAGCAGAG AAGGGGGACG TAGACATCAC CATCAGAGCT CGGTGGATGA GGGCGTAGGA
    GGTGGAGAGA GGGAACATCG GCACCATCAC TCCCATAGGC ATAACAGAGA GGGCAATGGT
    GTGGTCAGTG CTGGGGGGAA GAGTGAACGG CGGTCCAGGC AGAAAGAGGG CTCACGATCA
    GGGACAAGAG AGGGAGAGAG ATGCTCAAGG GGGGAGAATG GAGGAAATGG AGGGAGGAGG
    CGACACAGGC CACGAACCAG TAAAGCTCAG TCTACGCTGG AAGGAGAGGA GCAACATGTG
    AATGGAGAGG AGGAGGGCAA GTCGCAAAGC CATAGGCACA TGGATATGGG AGGAGATTCA
    ATGGCTGTAA ACCCCCATCA ACCTTCAGTT GGAAGCCGGT GGTGTCTTGA GAAACCAGAA
    GATTCGGACA ACAAACGAAA TATTGGTCAG ACTGGAGGAA TGAGCACCCA TATACCCGTG
    ACTGTCACTT CTCCCCCGGG AGAAACAACC CTTATTCCCA TGAACAGTAT AGACAGCGAG
    ACAGTGCCCA TGACTGAGAA GAATCTAGAA GATCTAAATC AAAGCAGCAT TCGTCCCATC
    CTCCCCTTCA GCTCCATGTT CATTTTCAAC CCAACTAATC CGGTGAGACG TTTATGCCAC
    TACATTGTGA GTCTGCGCTA TTTTGAGATG TGTATCCTGG TGGTCATTGC CATGAGCAGT
    ATAGCCCTGG CTGCAGAAGA CCCGGTCCAA GCCAATGCAC CTCGGAACAA TGTCCTGAAG
    TATTTGGACT ATGTCTTCAC TGGTGTTTTC ACATTCGAGA TGGTCATTAA GATGGTGGAT
    TTAGGTTTAA TTCTGCATCC TGGTTCCTAC TTTCGAGATC TATGGAATAT TTTGGACTTC
    ATTGTGGTCA GTGGTGCACT GGTGGCATTC GCATTCTCGA GCTTCATGGG ATCACATCAA
    AGCGTAAACA GAGGAACCAA GGGCAAAGAC ATCAGCACTA TCAAGTCACT GAGGGTGTTG
    CGAGTCTTGC GACCTCTGAA GACCATCAAA CGTCTACCAA AACTGAAGGC CGTGTTTGAT
    TGTGTGGTGA ACTCCCTGAA GAATGTCCTG AATATCCTCA TTGTCTACAT TCTGTTCATG
    TTCATTTTCG CTGTCATTGC TGTGCAGCTC TTCAAGGGCA AGTTTTTTTA CTGCACAGAC
    GAGTCCAAGG GCCTGGAGAA AGACTGCAGG GGGCTGTTTT TGGATTATGA TAAGGATGAT
    GTGACGGCAC AACCGAGGGA ATGGAAGAAA TACGAGTTTC ACTATGACAA TGTGCTTTGG
    GCTTTTCTCA CTCTCTTCAC TGTTTCTACG GGAGAGGGCT GGCCTACGGT TTTGAAGCAC
    TCCGTGGATG CCACTTTTGA GGATCAGGGT CCCAGTCCAG GCTACCGCAT TGAGATGTCT
    ATTTTTTACG TGGTCTACTT TGTGGTTTTC CCTTTCTTCT TCGTCAACAT TTTTGTAGCC
    TTGATAATCA TCACATTCCA GGAACAGGGT GATAAGGTCT TGTCAGAGTG CAGTCTGGAG
    AAGAATGAGA GAGCCTGCAT CGACTTTGCT ATCAATGCCA AACCTCTGAC ACGCTACATG
    CCGCAGAACC AGCAGTCCTT TCAGTACCGC CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT
    GAGTACTCCA TCATGATCAT GATTGCTCTC AACACTGTGG TGCTCATGAT GAAGTTCCAT
    GGGGCGCCTG ACTTTTATGA GGCAATGCTT AAGTATCTCA ACATTGTCTT CACTGTTCTC
    TTTTCACTGG AGTGTATTCT CAAGATTATT GCTTTCGGCC CCTTGAATTA CCTGAAAGAT
    GCCTGGAATG TGTTTGACTT TGTGACTGTG CTGGGCAGCA TCACTGACAT TTTAGTCACT
    GAGATTAACA CAACGGAGCG TCAGCTGAAC TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC
    CGACTCATCA AGCTCCTCCG GCAGGGATAC ACCATTCGCA TCCTGCTCTG GACATTTGTC
    CAGTCTTTTA AGGCACTGCC TTATGTTTGC CTTCTAATCG CCATGCTGTT TTTCATCTAC
    GCCATTATTG GGATGCAGGT GTTTGGAAAC ATTGATTTGA ATGATGACAC GGCAATCAAC
    CGTCACAACA ACTTCCGCAC TTTCCTCCAA GCTCTTATGC TTTTGTTCAG GAGTGCCACT
    GGCGAGGCTT GGCATGATAT CATGCTGTCG TGTCTGAGCG AGCGCACCTG TGATCCGAGC
    TCAGGCACCC TTGGAAAGGA GTGTGGGAGT GACTTTGCCT ACTTCTACTT TGTCTCCTTC
    ATTTTCCTCT GCTCATTTCT GATGTTGAAC CTGTTTGTGG CCGTCATTAT GGACAACTTT
    GAATATCTGA CACGCGACTC CTCCATCCTG GGCCCTCACC ACCTGGATGA GTTTATTCGA
    GTGTGGGCGG AGTATGACCC AGCCGCATGT GGCCGTATCA AGTACCTCGA TATGTATGAG
    ATGCTGCTCC ACATGTCCCC ACCTTTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC
    TACAAGCGTC TGGTGAAAAT GAACATGCCC ATTGCGGATG ACAACAGTGT ACACTTTACC
    TCAACGCTGA TGGCTCTGAT CCGTACTGCC CTGGAGATCA AACTGGCTTC AGGCATGGTG
    GCTCAGCGTC TGTCAGATGC TGAACTGAAG AAGGAGCTCT CTACAGTTTG GCCAAACCTG
    TCTCAGAAGA CCATGGACCT GCTGGTCACT CCACATAAAC CTAATGAGCT CACAGTGGGG
    AAGGTGTATG CAGCTCTTAT GATCTTTGAC TATTACAAGC AGAACAGAGC AAAAAGACTT
    CAGATGCAGC AACAGCAGCT GAAAGAACAG GCAGGACCTG GATCTCAGAA TAAAGTCAGC
    GCTCTCTTAG AGCCCATTCT CCCACTAACC CACATGCAAG ATCAGCCAGT GAACGGTGTC
    GATTCAGCTT CAGAGTGTCA AGCTCAGACC AGACCCAGCT CCACCACACT CAACAATGGA
    CGCACAACTC TTGACAACAC AATAAAAGGG TCTTCATCCT GGGCCTCAGA GAAATCCAAG
    GAAGTCCAAA AGTCTAAACG GAGACCAATG TCTAGAGGTC AATCTGAGGA TGCCTCAAAT
    GCAAACACTG CCCAGGAGTC AGTAGAAATG AGAAAGATGG AGAACAGTGC CAACACAGTG
    ACCTCTGGTG GCCTTGAGGG CCAAGGACGT GCAGCGTCCA TGCCAAAACT CAATGCTGAG
    ATGCAGAGGA GTCATTCCCG TCAATCTCCT GGAACATTAC TGGAACCCAT TCCAGATTCC
    CCCATGAGGC GCTCTGCATC CACTTTTGCA CCACAGCGCC CTCACGAGGT GAATCTGAAT
    GACTACATTC TGGAGAAACC TGTTCAGGAA CGCCATCACC ACCATCACCA TCATCACCGA
    TGCCATCATC GCCGGGACAG AGACAAGAAA CAGAGATCTC TGGACAGGTC GCCTACTGGC
    CATCACAAAA CTACAGGCAC AGCCACTGAC TCTGTAGAGG AGAGGGCACA TGACCGGGGT
    CGTTCCCATG AGAGGAAGCA CCATTCATCT TCAGGAGAGA AGCAGCGGTA TTACTCGTGT
    GATCGCTATG CCAGTCGGGA ACACTGTTCC AGCAAATCTG CCGTGGCAAG CTGTGCCACC
    TCACCCAGCG AGACCCAGGA GGCCAATCTT AACAAGCAGG GCAGTGGGTG GGCTAAGGGG
    AGCCCGGTGT TGTTGACTTC AGGGGCGAGC ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC
    CAGACCCCCC TCACTCCTCG TCCCTCCGTG GCCTACAGGA CTGCCAACTC TTCCCCTGTG
    CAACTTCTGG CCACAACGAC CAGCTTGCTT CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT
    CTGTCCGAGC ACAACGCTCT TTTACTCAGC GGCTCTATCT CCAGCTCGCC GGCCCCCGTC
    AGTCGCATTA ACTCAGAACC CTTCTTGGGG CCCCCGGACC ACCCTGCTGG CTTGCAGCCC
    GAAGCCTTCG CTGTCTTCCA AGAGAATCCG AGCCCTCAGA TGGGCCGTTC TCCTCAGACT
    GTAGGGATGC CCTTCGTGGC GATCCCCCCA CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC
    AACGGGTACC ACTTCTCCAC GGGCCCCAGC GCCAGCAGAG GACGGGCACA GCAGTACTAC
    CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211584.1
    CDS1..7110
    Translation

    Target ORF information:

    RefSeq Version XM_017356095.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356095.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CGAGTTATGT CTGCTCCCCT
    GCACATCACT ACTTGAGTAA AGAACAGCAG CGTTCTCTAA AAATGATGTC CGTGTGGGAG
    CAGCGCACCA CTCAGATCCG AAAGCACATG CTAGCCAGCA GTGAGGCGTT GTACCAAGAA
    GAGCTCAATC CACGGCTCAT GTCTAACCTT CCTCTACGCA CCGACATAAA AACCCACCTG
    GACCGGCCTT TGGTTGTGGA GCCTCGCTGT GATGGCCCCA TCAGCCCACA CAGTGGCTGG
    GACAAACCCC AGGATTTGCA AGGCGAGGGC AGCACACCAG AAGAACGAGT CCCACCTGTA
    TTCGAGCCTC CACAGTCACA TCCACCACGC AAGCACCACC GTCACAGAGA GCGTCTTTCT
    AATGAGACCA ATGAAAATGG AGACACGGGT CATGCCAAAG AAGGCCGGCA TCACGTTCAT
    CACAGCCGAA GTAAAGAGCA TGATGGGACA CGTTGTAAGG AGGGGAAGGG TGACAGGAGC
    CGAAGCAGAG AAGGGGGACG TAGACATCAC CATCAGAGCT CGGTGGATGA GGGCGTAGGA
    GGTGGAGAGA GGGAACATCG GCACCATCAC TCCCATAGGC ATAACAGAGA GGGCAATGGT
    GTGGTCAGTG CTGGGGGGAA GAGTGAACGG CGGTCCAGGC AGAAAGAGGG CTCACGATCA
    GGGACAAGAG AGGGAGAGAG ATGCTCAAGG GGGGAGAATG GAGGAAATGG AGGGAGGAGG
    CGACACAGGC CACGAACCAG TAAAGCTCAG TCTACGCTGG AAGGAGAGGA GCAACATGTG
    AATGGAGAGG AGGAGGGCAA GTCGCAAAGC CATAGGCACA TGGATATGGG AGGAGATTCA
    ATGGCTGTAA ACCCCCATCA ACCTTCAGTT GGAAGCCGGT GGTGTCTTGA GAAACCAGAA
    GATTCGGACA ACAAACGAAA TATTGGTCAG ACTGGAGGAA TGAGCACCCA TATACCCGTG
    ACTGTCACTT CTCCCCCGGG AGAAACAACC CTTATTCCCA TGAACAGTAT AGACAGCGAG
    ACAGTGCCCA TGACTGAGAA GAATCTAGAA GATCTAAATC AAAGCAGCAT TCGTCCCATC
    CTCCCCTTCA GCTCCATGTT CATTTTCAAC CCAACTAATC CGGTGAGACG TTTATGCCAC
    TACATTGTGA GTCTGCGCTA TTTTGAGATG TGTATCCTGG TGGTCATTGC CATGAGCAGT
    ATAGCCCTGG CTGCAGAAGA CCCGGTCCAA GCCAATGCAC CTCGGAACAA TGTCCTGAAG
    TATTTGGACT ATGTCTTCAC TGGTGTTTTC ACATTCGAGA TGGTCATTAA GATGGTGGAT
    TTAGGTTTAA TTCTGCATCC TGGTTCCTAC TTTCGAGATC TATGGAATAT TTTGGACTTC
    ATTGTGGTCA GTGGTGCACT GGTGGCATTC GCATTCTCGA GCTTCATGGG ATCACATCAA
    AGCGTAAACA GAGGAACCAA GGGCAAAGAC ATCAGCACTA TCAAGTCACT GAGGGTGTTG
    CGAGTCTTGC GACCTCTGAA GACCATCAAA CGTCTACCAA AACTGAAGGC CGTGTTTGAT
    TGTGTGGTGA ACTCCCTGAA GAATGTCCTG AATATCCTCA TTGTCTACAT TCTGTTCATG
    TTCATTTTCG CTGTCATTGC TGTGCAGCTC TTCAAGGGCA AGTTTTTTTA CTGCACAGAC
    GAGTCCAAGG GCCTGGAGAA AGACTGCAGG GGGCTGTTTT TGGATTATGA TAAGGATGAT
    GTGACGGCAC AACCGAGGGA ATGGAAGAAA TACGAGTTTC ACTATGACAA TGTGCTTTGG
    GCTTTTCTCA CTCTCTTCAC TGTTTCTACG GGAGAGGGCT GGCCTACGGT TTTGAAGCAC
    TCCGTGGATG CCACTTTTGA GGATCAGGGT CCCAGTCCAG GCTACCGCAT TGAGATGTCT
    ATTTTTTACG TGGTCTACTT TGTGGTTTTC CCTTTCTTCT TCGTCAACAT TTTTGTAGCC
    TTGATAATCA TCACATTCCA GGAACAGGGT GATAAGGTCT TGTCAGAGTG CAGTCTGGAG
    AAGAATGAGA GAGCCTGCAT CGACTTTGCT ATCAATGCCA AACCTCTGAC ACGCTACATG
    CCGCAGAACC AGCAGTCCTT TCAGTACCGC CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT
    GAGTACTCCA TCATGATCAT GATTGCTCTC AACACTGTGG TGCTCATGAT GAAGTTCCAT
    GGGGCGCCTG ACTTTTATGA GGCAATGCTT AAGTATCTCA ACATTGTCTT CACTGTTCTC
    TTTTCACTGG AGTGTATTCT CAAGATTATT GCTTTCGGCC CCTTGAATTA CCTGAAAGAT
    GCCTGGAATG TGTTTGACTT TGTGACTGTG CTGGGCAGCA TCACTGACAT TTTAGTCACT
    GAGATTAACA CAACGGAGCG TCAGCTGAAC TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC
    CGACTCATCA AGCTCCTCCG GCAGGGATAC ACCATTCGCA TCCTGCTCTG GACATTTGTC
    CAGTCTTTTA AGGCACTGCC TTATGTTTGC CTTCTAATCG CCATGCTGTT TTTCATCTAC
    GCCATTATTG GGATGCAGGT GTTTGGAAAC ATTGATTTGA ATGATGACAC GGCAATCAAC
    CGTCACAACA ACTTCCGCAC TTTCCTCCAA GCTCTTATGC TTTTGTTCAG GAGTGCCACT
    GGCGAGGCTT GGCATGATAT CATGCTGTCG TGTCTGAGCG AGCGCACCTG TGATCCGAGC
    TCAGGCACCC TTGGAAAGGA GTGTGGGAGT GACTTTGCCT ACTTCTACTT TGTCTCCTTC
    ATTTTCCTCT GCTCATTTCT GATGTTGAAC CTGTTTGTGG CCGTCATTAT GGACAACTTT
    GAATATCTGA CACGCGACTC CTCCATCCTG GGCCCTCACC ACCTGGATGA GTTTATTCGA
    GTGTGGGCGG AGTATGACCC AGCCGCATGT GGCCGTATCA AGTACCTCGA TATGTATGAG
    ATGCTGCTCC ACATGTCCCC ACCTTTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC
    TACAAGCGTC TGGTGAAAAT GAACATGCCC ATTGCGGATG ACAACAGTGT ACACTTTACC
    TCAACGCTGA TGGCTCTGAT CCGTACTGCC CTGGAGATCA AACTGGCTTC AGGCATGGTG
    GCTCAGCGTC TGTCAGATGC TGAACTGAAG AAGGAGCTCT CTACAGTTTG GCCAAACCTG
    TCTCAGAAGA CCATGGACCT GCTGGTCACT CCACATAAAC CTAATGAGCT CACAGTGGGG
    AAGGTGTATG CAGCTCTTAT GATCTTTGAC TATTACAAGC AGAACAGAGC AAAAAGACTT
    CAGATGCAGC AACAGCAGCT GAAAGAACAG GCAGGACCTG GATCTCAGAA TAAAGTCAGC
    GCTCTCTTAG AGCCCATTCT CCCACTAACC CACATGCAAG ATCAGCCAGT GAACGGTGTC
    GATTCAGCTT CAGAGTGTCA AGCTCAGACC AGACCCAGCT CCACCACACT CAACAATGGA
    CGCACAACTC TTGACAACAC AATAAAAGGG TCTTCATCCT GGGCCTCAGA GAAATCCAAG
    GAAGTCCAAA AGTCTAAACG GAGACCAATG TCTAGAGGTC AATCTGAGGA TGCCTCAAAT
    GCAAACACTG CCCAGGAGTC AGTAGAAATG AGAAAGATGG AGAACAGTGC CAACACAGTG
    ACCTCTGGTG GCCTTGAGGG CCAAGGACGT GCAGCGTCCA TGCCAAAACT CAATGCTGAG
    ATGCAGAGGA GTCATTCCCG TCAATCTCCT GGAACATTAC TGGAACCCAT TCCAGATTCC
    CCCATGAGGC GCTCTGCATC CACTTTTGCA CCACAGCGCC CTCACGAGGT GAATCTGAAT
    GACTACATTC TGGAGAAACC TGTTCAGGAA CGCCATCACC ACCATCACCA TCATCACCGA
    TGCCATCATC GCCGGGACAG AGACAAGAAA CAGAGATCTC TGGACAGGTC GCCTACTGGC
    CATCACAAAA CTACAGGCAC AGCCACTGAC TCTGTAGAGG AGAGGGCACA TGACCGGGGT
    CGTTCCCATG AGAGGAAGCA CCATTCATCT TCAGGAGAGA AGCAGCGGTA TTACTCGTGT
    GATCGCTATG CCAGTCGGGA ACACTGTTCC AGCAAATCTG CCGTGGCAAG CTGTGCCACC
    TCACCCAGCG AGACCCAGGA GGCCAATCTT AACAAGCAGG GCAGTGGGTG GGCTAAGGGG
    AGCCCGGTGT TGTTGACTTC AGGGGCGAGC ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC
    CAGACCCCCC TCACTCCTCG TCCCTCCGTG GCCTACAGGA CTGCCAACTC TTCCCCTGTG
    CAACTTCTGG CCACAACGAC CAGCTTGCTT CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT
    CTGTCCGAGC ACAACGCTCT TTTACTCAGC GGCTCTATCT CCAGCTCGCC GGCCCCCGTC
    AGTCGCATTA ACTCAGAACC CTTCTTGGGG CCCCCGGACC ACCCTGCTGG CTTGCAGCCC
    GAAGCCTTCG CTGTCTTCCA AGAGAATCCG AGCCCTCAGA TGGGCCGTTC TCCTCAGACT
    GTAGGGATGC CCTTCGTGGC GATCCCCCCA CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC
    AACGGGTACC ACTTCTCCAC GGGCCCCAGC GCCAGCAGAG GACGGGCACA GCAGTACTAC
    CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59086
    Clone ID Related Accession (Same CDS sequence) XM_017356096.2 , XM_017356096.1
    Accession Version XM_017356096.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG
    ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG
    CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT
    GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT
    TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG
    TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA
    AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA
    GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG
    GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA
    CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG
    GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA
    GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA
    ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG
    GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC
    CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA
    CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC
    CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT
    GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC
    ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG
    CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA
    GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC
    TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG
    CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT
    GCACTGGTGG CATTCGCATT CTCGAGCTTC ATGGGATCAC ATCAAAGCGT AAACAGAGGA
    ACCAAGGGCA AAGACATCAG CACTATCAAG TCACTGAGGG TGTTGCGAGT CTTGCGACCT
    CTGAAGACCA TCAAACGTCT ACCAAAACTG AAGGCCGTGT TTGATTGTGT GGTGAACTCC
    CTGAAGAATG TCCTGAATAT CCTCATTGTC TACATTCTGT TCATGTTCAT TTTCGCTGTC
    ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT TTTTACTGCA CAGACGAGTC CAAGGGCCTG
    GAGAAAGACT GCAGGGGGCT GTTTTTGGAT TATGATAAGG ATGATGTGAC GGCACAACCG
    AGGGAATGGA AGAAATACGA GTTTCACTAT GACAATGTGC TTTGGGCTTT TCTCACTCTC
    TTCACTGTTT CTACGGGAGA GGGCTGGCCT ACGGTTTTGA AGCACTCCGT GGATGCCACT
    TTTGAGGATC AGGGTCCCAG TCCAGGCTAC CGCATTGAGA TGTCTATTTT TTACGTGGTC
    TACTTTGTGG TTTTCCCTTT CTTCTTCGTC AACATTTTTG TAGCCTTGAT AATCATCACA
    TTCCAGGAAC AGGGTGATAA GGTCTTGTCA GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC
    TGCATCGACT TTGCTATCAA TGCCAAACCT CTGACACGCT ACATGCCGCA GAACCAGCAG
    TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG GTCTCTCCGC CGTTTGAGTA CTCCATCATG
    ATCATGATTG CTCTCAACAC TGTGGTGCTC ATGATGAAGT TCCATGGGGC GCCTGACTTT
    TATGAGGCAA TGCTTAAGTA TCTCAACATT GTCTTCACTG TTCTCTTTTC ACTGGAGTGT
    ATTCTCAAGA TTATTGCTTT CGGCCCCTTG AATTACCTGA AAGATGCCTG GAATGTGTTT
    GACTTTGTGA CTGTGCTGGG CAGCATCACT GACATTTTAG TCACTGAGAT TAACACAACG
    GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC
    CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA
    CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG
    CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC
    CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT
    GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA
    AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA
    TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC
    GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT
    GACCCAGCCG CATGTGGCCG TATCAAGTAC CTCGATATGT ATGAGATGCT GCTCCACATG
    TCCCCACCTT TAGGTTTGGG AAAGAAATGT CCTCCACGAA TCGCCTACAA GCGTCTGGTG
    AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT
    CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA
    GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG
    GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT
    CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG
    CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC
    ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG
    TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC
    AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT
    AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG
    GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT
    GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT
    TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT
    GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG
    AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG
    GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA
    GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG
    AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT
    CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC
    CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG
    ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT
    CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA
    ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC
    GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA
    GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC
    TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC
    GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC
    TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG
    GAGGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211585.1
    CDS1..7095
    Translation

    Target ORF information:

    RefSeq Version XM_017356096.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356096.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG
    ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG
    CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT
    GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT
    TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG
    TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA
    AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA
    GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG
    GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA
    CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG
    GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA
    GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA
    ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG
    GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC
    CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA
    CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC
    CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT
    GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC
    ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG
    CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA
    GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC
    TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG
    CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT
    GCACTGGTGG CATTCGCATT CTCGAGCTTC ATGGGATCAC ATCAAAGCGT AAACAGAGGA
    ACCAAGGGCA AAGACATCAG CACTATCAAG TCACTGAGGG TGTTGCGAGT CTTGCGACCT
    CTGAAGACCA TCAAACGTCT ACCAAAACTG AAGGCCGTGT TTGATTGTGT GGTGAACTCC
    CTGAAGAATG TCCTGAATAT CCTCATTGTC TACATTCTGT TCATGTTCAT TTTCGCTGTC
    ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT TTTTACTGCA CAGACGAGTC CAAGGGCCTG
    GAGAAAGACT GCAGGGGGCT GTTTTTGGAT TATGATAAGG ATGATGTGAC GGCACAACCG
    AGGGAATGGA AGAAATACGA GTTTCACTAT GACAATGTGC TTTGGGCTTT TCTCACTCTC
    TTCACTGTTT CTACGGGAGA GGGCTGGCCT ACGGTTTTGA AGCACTCCGT GGATGCCACT
    TTTGAGGATC AGGGTCCCAG TCCAGGCTAC CGCATTGAGA TGTCTATTTT TTACGTGGTC
    TACTTTGTGG TTTTCCCTTT CTTCTTCGTC AACATTTTTG TAGCCTTGAT AATCATCACA
    TTCCAGGAAC AGGGTGATAA GGTCTTGTCA GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC
    TGCATCGACT TTGCTATCAA TGCCAAACCT CTGACACGCT ACATGCCGCA GAACCAGCAG
    TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG GTCTCTCCGC CGTTTGAGTA CTCCATCATG
    ATCATGATTG CTCTCAACAC TGTGGTGCTC ATGATGAAGT TCCATGGGGC GCCTGACTTT
    TATGAGGCAA TGCTTAAGTA TCTCAACATT GTCTTCACTG TTCTCTTTTC ACTGGAGTGT
    ATTCTCAAGA TTATTGCTTT CGGCCCCTTG AATTACCTGA AAGATGCCTG GAATGTGTTT
    GACTTTGTGA CTGTGCTGGG CAGCATCACT GACATTTTAG TCACTGAGAT TAACACAACG
    GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC
    CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA
    CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG
    CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC
    CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT
    GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA
    AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA
    TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC
    GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT
    GACCCAGCCG CATGTGGCCG TATCAAGTAC CTCGATATGT ATGAGATGCT GCTCCACATG
    TCCCCACCTT TAGGTTTGGG AAAGAAATGT CCTCCACGAA TCGCCTACAA GCGTCTGGTG
    AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT
    CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA
    GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG
    GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT
    CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG
    CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC
    ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG
    TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC
    AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT
    AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG
    GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT
    GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT
    TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT
    GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG
    AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG
    GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA
    GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG
    AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT
    CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC
    CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG
    ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT
    CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA
    ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC
    GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA
    GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC
    TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC
    GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC
    TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG
    GAGGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59086
    Clone ID Related Accession (Same CDS sequence) XM_017356096.2 , XM_017356096.1
    Accession Version XM_017356096.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG
    ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG
    CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT
    GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT
    TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG
    TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA
    AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA
    GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG
    GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA
    CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG
    GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA
    GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA
    ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG
    GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC
    CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA
    CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC
    CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT
    GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC
    ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG
    CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA
    GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC
    TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG
    CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT
    GCACTGGTGG CATTCGCATT CTCGAGCTTC ATGGGATCAC ATCAAAGCGT AAACAGAGGA
    ACCAAGGGCA AAGACATCAG CACTATCAAG TCACTGAGGG TGTTGCGAGT CTTGCGACCT
    CTGAAGACCA TCAAACGTCT ACCAAAACTG AAGGCCGTGT TTGATTGTGT GGTGAACTCC
    CTGAAGAATG TCCTGAATAT CCTCATTGTC TACATTCTGT TCATGTTCAT TTTCGCTGTC
    ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT TTTTACTGCA CAGACGAGTC CAAGGGCCTG
    GAGAAAGACT GCAGGGGGCT GTTTTTGGAT TATGATAAGG ATGATGTGAC GGCACAACCG
    AGGGAATGGA AGAAATACGA GTTTCACTAT GACAATGTGC TTTGGGCTTT TCTCACTCTC
    TTCACTGTTT CTACGGGAGA GGGCTGGCCT ACGGTTTTGA AGCACTCCGT GGATGCCACT
    TTTGAGGATC AGGGTCCCAG TCCAGGCTAC CGCATTGAGA TGTCTATTTT TTACGTGGTC
    TACTTTGTGG TTTTCCCTTT CTTCTTCGTC AACATTTTTG TAGCCTTGAT AATCATCACA
    TTCCAGGAAC AGGGTGATAA GGTCTTGTCA GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC
    TGCATCGACT TTGCTATCAA TGCCAAACCT CTGACACGCT ACATGCCGCA GAACCAGCAG
    TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG GTCTCTCCGC CGTTTGAGTA CTCCATCATG
    ATCATGATTG CTCTCAACAC TGTGGTGCTC ATGATGAAGT TCCATGGGGC GCCTGACTTT
    TATGAGGCAA TGCTTAAGTA TCTCAACATT GTCTTCACTG TTCTCTTTTC ACTGGAGTGT
    ATTCTCAAGA TTATTGCTTT CGGCCCCTTG AATTACCTGA AAGATGCCTG GAATGTGTTT
    GACTTTGTGA CTGTGCTGGG CAGCATCACT GACATTTTAG TCACTGAGAT TAACACAACG
    GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC
    CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA
    CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG
    CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC
    CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT
    GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA
    AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA
    TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC
    GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT
    GACCCAGCCG CATGTGGCCG TATCAAGTAC CTCGATATGT ATGAGATGCT GCTCCACATG
    TCCCCACCTT TAGGTTTGGG AAAGAAATGT CCTCCACGAA TCGCCTACAA GCGTCTGGTG
    AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT
    CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA
    GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG
    GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT
    CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG
    CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC
    ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG
    TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC
    AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT
    AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG
    GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT
    GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT
    TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT
    GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG
    AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG
    GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA
    GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG
    AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT
    CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC
    CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG
    ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT
    CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA
    ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC
    GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA
    GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC
    TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC
    GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC
    TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG
    GAGGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211585.1
    CDS636..7730
    Translation

    Target ORF information:

    RefSeq Version XM_017356096.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356096.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG
    ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG
    CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT
    GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT
    TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG
    TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA
    AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA
    GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG
    GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA
    CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG
    GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA
    GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA
    ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG
    GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC
    CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA
    CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC
    CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT
    GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC
    ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG
    CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA
    GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC
    TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG
    CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT
    GCACTGGTGG CATTCGCATT CTCGAGCTTC ATGGGATCAC ATCAAAGCGT AAACAGAGGA
    ACCAAGGGCA AAGACATCAG CACTATCAAG TCACTGAGGG TGTTGCGAGT CTTGCGACCT
    CTGAAGACCA TCAAACGTCT ACCAAAACTG AAGGCCGTGT TTGATTGTGT GGTGAACTCC
    CTGAAGAATG TCCTGAATAT CCTCATTGTC TACATTCTGT TCATGTTCAT TTTCGCTGTC
    ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT TTTTACTGCA CAGACGAGTC CAAGGGCCTG
    GAGAAAGACT GCAGGGGGCT GTTTTTGGAT TATGATAAGG ATGATGTGAC GGCACAACCG
    AGGGAATGGA AGAAATACGA GTTTCACTAT GACAATGTGC TTTGGGCTTT TCTCACTCTC
    TTCACTGTTT CTACGGGAGA GGGCTGGCCT ACGGTTTTGA AGCACTCCGT GGATGCCACT
    TTTGAGGATC AGGGTCCCAG TCCAGGCTAC CGCATTGAGA TGTCTATTTT TTACGTGGTC
    TACTTTGTGG TTTTCCCTTT CTTCTTCGTC AACATTTTTG TAGCCTTGAT AATCATCACA
    TTCCAGGAAC AGGGTGATAA GGTCTTGTCA GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC
    TGCATCGACT TTGCTATCAA TGCCAAACCT CTGACACGCT ACATGCCGCA GAACCAGCAG
    TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG GTCTCTCCGC CGTTTGAGTA CTCCATCATG
    ATCATGATTG CTCTCAACAC TGTGGTGCTC ATGATGAAGT TCCATGGGGC GCCTGACTTT
    TATGAGGCAA TGCTTAAGTA TCTCAACATT GTCTTCACTG TTCTCTTTTC ACTGGAGTGT
    ATTCTCAAGA TTATTGCTTT CGGCCCCTTG AATTACCTGA AAGATGCCTG GAATGTGTTT
    GACTTTGTGA CTGTGCTGGG CAGCATCACT GACATTTTAG TCACTGAGAT TAACACAACG
    GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC
    CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA
    CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG
    CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC
    CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT
    GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA
    AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA
    TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC
    GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT
    GACCCAGCCG CATGTGGCCG TATCAAGTAC CTCGATATGT ATGAGATGCT GCTCCACATG
    TCCCCACCTT TAGGTTTGGG AAAGAAATGT CCTCCACGAA TCGCCTACAA GCGTCTGGTG
    AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT
    CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA
    GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG
    GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT
    CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG
    CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC
    ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG
    TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC
    AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT
    AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG
    GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT
    GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT
    TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT
    GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG
    AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG
    GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA
    GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG
    AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT
    CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC
    CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG
    ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT
    CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA
    ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC
    GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA
    GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC
    TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC
    GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC
    TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG
    GAGGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59087
    Clone ID Related Accession (Same CDS sequence) XM_017356097.1 , XM_017356097.2
    Accession Version XM_017356097.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC
    ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA
    CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC
    CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG
    GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG
    TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG
    TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG
    GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT
    CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC
    TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG
    GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT
    GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG
    AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT
    GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT
    CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC
    GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC
    AGCATCACTG ACATTTTAGT CACTGAGATT AACGAGCGTC AGCTGAACTT CAGTTTCCTG
    AGGCTCTTTA GAGCAGCCCG ACTCATCAAG CTCCTCCGGC AGGGATACAC CATTCGCATC
    CTGCTCTGGA CATTTGTCCA GTCTTTTAAG GCACTGCCTT ATGTTTGCCT TCTAATCGCC
    ATGCTGTTTT TCATCTACGC CATTATTGGG ATGCAGGTGT TTGGAAACAT TGATTTGAAT
    GATGACACGG CAATCAACCG TCACAACAAC TTCCGCACTT TCCTCCAAGC TCTTATGCTT
    TTGTTCAGGA GTGCCACTGG CGAGGCTTGG CATGATATCA TGCTGTCGTG TCTGAGCGAG
    CGCACCTGTG ATCCGAGCTC AGGCACCCTT GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC
    TTCTACTTTG TCTCCTTCAT TTTCCTCTGC TCATTTCTGA TGTTGAACCT GTTTGTGGCC
    GTCATTATGG ACAACTTTGA ATATCTGACA CGCGACTCCT CCATCCTGGG CCCTCACCAC
    CTGGATGAGT TTATTCGAGT GTGGGCGGAG TATGACCCAG CCGCATGTGG CCGTATCAAG
    TACCTCGATA TGTATGAGAT GCTGCTCCAC ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA
    TGTCCTCCAC GAATCGCCTA CAAGCGTCTG GTGAAAATGA ACATGCCCAT TGCGGATGAC
    AACAGTGTAC ACTTTACCTC AACGCTGATG GCTCTGATCC GTACTGCCCT GGAGATCAAA
    CTGGCTTCAG GCATGGTGGC TCAGCGTCTG TCAGATGCTG AACTGAAGAA GGAGCTCTCT
    ACAGTTTGGC CAAACCTGTC TCAGAAGACC ATGGACCTGC TGGTCACTCC ACATAAACCT
    AATGAGCTCA CAGTGGGGAA GGTGTATGCA GCTCTTATGA TCTTTGACTA TTACAAGCAG
    AACAGAGCAA AAAGACTTCA GATGCAGCAA CAGCAGCTGA AAGAACAGGC AGGACCTGGA
    TCTCAGAATA AAGTCAGCGC TCTCTTAGAG CCCATTCTCC CACTAACCCA CATGCAAGAT
    CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA GAGTGTCAAG CTCAGACCAG ACCCAGCTCC
    ACCACACTCA ACAATGGACG CACAACTCTT GACAACACAA TAAAAGGGTC TTCATCCTGG
    GCCTCAGAGA AATCCAAGGA AGTCCAAAAG TCTAAACGGA GACCAATGTC TAGAGGTCAA
    TCTGAGGATG CCTCAAATGC AAACACTGCC CAGGAGTCAG TAGAAATGAG AAAGATGGAG
    AACAGTGCCA ACACAGTGAC CTCTGGTGGC CTTGAGGGCC AAGGACGTGC AGCGTCCATG
    CCAAAACTCA ATGCTGAGAT GCAGAGGAGT CATTCCCGTC AATCTCCTGG AACATTACTG
    GAACCCATTC CAGATTCCCC CATGAGGCGC TCTGCATCCA CTTTTGCACC ACAGCGCCCT
    CACGAGGTGA ATCTGAATGA CTACATTCTG GAGAAACCTG TTCAGGAACG CCATCACCAC
    CATCACCATC ATCACCGATG CCATCATCGC CGGGACAGAG ACAAGAAACA GAGATCTCTG
    GACAGGTCGC CTACTGGCCA TCACAAAACT ACAGGCACAG CCACTGACTC TGTAGAGGAG
    AGGGCACATG ACCGGGGTCG TTCCCATGAG AGGAAGCACC ATTCATCTTC AGGAGAGAAG
    CAGCGGTATT ACTCGTGTGA TCGCTATGCC AGTCGGGAAC ACTGTTCCAG CAAATCTGCC
    GTGGCAAGCT GTGCCACCTC ACCCAGCGAG ACCCAGGAGG CCAATCTTAA CAAGCAGGGC
    AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG TTGACTTCAG GGGCGAGCAC TCCAAGCCGC
    GGGCGCAGAC AGCTGCCCCA GACCCCCCTC ACTCCTCGTC CCTCCGTGGC CTACAGGACT
    GCCAACTCTT CCCCTGTGCA ACTTCTGGCC ACAACGACCA GCTTGCTTCC CCCGAGTCCC
    GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC AACGCTCTTT TACTCAGCGG CTCTATCTCC
    AGCTCGCCGG CCCCCGTCAG TCGCATTAAC TCAGAACCCT TCTTGGGGCC CCCGGACCAC
    CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT GTCTTCCAAG AGAATCCGAG CCCTCAGATG
    GGCCGTTCTC CTCAGACTGT AGGGATGCCC TTCGTGGCGA TCCCCCCACC TCCCCCACAG
    CAGTTTCGCA GGGTCCCCAA CGGGTACCAC TTCTCCACGG GCCCCAGCGC CAGCAGAGGA
    CGGGCACAGC AGTACTACCC AGAGGCAGAG GTGGAGGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211586.1
    CDS636..7703
    Translation

    Target ORF information:

    RefSeq Version XM_017356097.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356097.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
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    6181
    6241
    6301
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    6421
    6481
    6541
    6601
    6661
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    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC
    ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA
    CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC
    CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG
    GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG
    TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG
    TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG
    GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT
    CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC
    TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG
    GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT
    GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG
    AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT
    GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT
    CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC
    GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC
    AGCATCACTG ACATTTTAGT CACTGAGATT AACGAGCGTC AGCTGAACTT CAGTTTCCTG
    AGGCTCTTTA GAGCAGCCCG ACTCATCAAG CTCCTCCGGC AGGGATACAC CATTCGCATC
    CTGCTCTGGA CATTTGTCCA GTCTTTTAAG GCACTGCCTT ATGTTTGCCT TCTAATCGCC
    ATGCTGTTTT TCATCTACGC CATTATTGGG ATGCAGGTGT TTGGAAACAT TGATTTGAAT
    GATGACACGG CAATCAACCG TCACAACAAC TTCCGCACTT TCCTCCAAGC TCTTATGCTT
    TTGTTCAGGA GTGCCACTGG CGAGGCTTGG CATGATATCA TGCTGTCGTG TCTGAGCGAG
    CGCACCTGTG ATCCGAGCTC AGGCACCCTT GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC
    TTCTACTTTG TCTCCTTCAT TTTCCTCTGC TCATTTCTGA TGTTGAACCT GTTTGTGGCC
    GTCATTATGG ACAACTTTGA ATATCTGACA CGCGACTCCT CCATCCTGGG CCCTCACCAC
    CTGGATGAGT TTATTCGAGT GTGGGCGGAG TATGACCCAG CCGCATGTGG CCGTATCAAG
    TACCTCGATA TGTATGAGAT GCTGCTCCAC ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA
    TGTCCTCCAC GAATCGCCTA CAAGCGTCTG GTGAAAATGA ACATGCCCAT TGCGGATGAC
    AACAGTGTAC ACTTTACCTC AACGCTGATG GCTCTGATCC GTACTGCCCT GGAGATCAAA
    CTGGCTTCAG GCATGGTGGC TCAGCGTCTG TCAGATGCTG AACTGAAGAA GGAGCTCTCT
    ACAGTTTGGC CAAACCTGTC TCAGAAGACC ATGGACCTGC TGGTCACTCC ACATAAACCT
    AATGAGCTCA CAGTGGGGAA GGTGTATGCA GCTCTTATGA TCTTTGACTA TTACAAGCAG
    AACAGAGCAA AAAGACTTCA GATGCAGCAA CAGCAGCTGA AAGAACAGGC AGGACCTGGA
    TCTCAGAATA AAGTCAGCGC TCTCTTAGAG CCCATTCTCC CACTAACCCA CATGCAAGAT
    CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA GAGTGTCAAG CTCAGACCAG ACCCAGCTCC
    ACCACACTCA ACAATGGACG CACAACTCTT GACAACACAA TAAAAGGGTC TTCATCCTGG
    GCCTCAGAGA AATCCAAGGA AGTCCAAAAG TCTAAACGGA GACCAATGTC TAGAGGTCAA
    TCTGAGGATG CCTCAAATGC AAACACTGCC CAGGAGTCAG TAGAAATGAG AAAGATGGAG
    AACAGTGCCA ACACAGTGAC CTCTGGTGGC CTTGAGGGCC AAGGACGTGC AGCGTCCATG
    CCAAAACTCA ATGCTGAGAT GCAGAGGAGT CATTCCCGTC AATCTCCTGG AACATTACTG
    GAACCCATTC CAGATTCCCC CATGAGGCGC TCTGCATCCA CTTTTGCACC ACAGCGCCCT
    CACGAGGTGA ATCTGAATGA CTACATTCTG GAGAAACCTG TTCAGGAACG CCATCACCAC
    CATCACCATC ATCACCGATG CCATCATCGC CGGGACAGAG ACAAGAAACA GAGATCTCTG
    GACAGGTCGC CTACTGGCCA TCACAAAACT ACAGGCACAG CCACTGACTC TGTAGAGGAG
    AGGGCACATG ACCGGGGTCG TTCCCATGAG AGGAAGCACC ATTCATCTTC AGGAGAGAAG
    CAGCGGTATT ACTCGTGTGA TCGCTATGCC AGTCGGGAAC ACTGTTCCAG CAAATCTGCC
    GTGGCAAGCT GTGCCACCTC ACCCAGCGAG ACCCAGGAGG CCAATCTTAA CAAGCAGGGC
    AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG TTGACTTCAG GGGCGAGCAC TCCAAGCCGC
    GGGCGCAGAC AGCTGCCCCA GACCCCCCTC ACTCCTCGTC CCTCCGTGGC CTACAGGACT
    GCCAACTCTT CCCCTGTGCA ACTTCTGGCC ACAACGACCA GCTTGCTTCC CCCGAGTCCC
    GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC AACGCTCTTT TACTCAGCGG CTCTATCTCC
    AGCTCGCCGG CCCCCGTCAG TCGCATTAAC TCAGAACCCT TCTTGGGGCC CCCGGACCAC
    CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT GTCTTCCAAG AGAATCCGAG CCCTCAGATG
    GGCCGTTCTC CTCAGACTGT AGGGATGCCC TTCGTGGCGA TCCCCCCACC TCCCCCACAG
    CAGTTTCGCA GGGTCCCCAA CGGGTACCAC TTCTCCACGG GCCCCAGCGC CAGCAGAGGA
    CGGGCACAGC AGTACTACCC AGAGGCAGAG GTGGAGGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59087
    Clone ID Related Accession (Same CDS sequence) XM_017356097.1 , XM_017356097.2
    Accession Version XM_017356097.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    301
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
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    4681
    4741
    4801
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    4981
    5041
    5101
    5161
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    5281
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    5401
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    5941
    6001
    6061
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    6181
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    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC
    ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA
    CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC
    CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG
    GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG
    TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG
    TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG
    GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT
    CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC
    TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG
    GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT
    GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG
    AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT
    GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT
    CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC
    GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC
    AGCATCACTG ACATTTTAGT CACTGAGATT AACGAGCGTC AGCTGAACTT CAGTTTCCTG
    AGGCTCTTTA GAGCAGCCCG ACTCATCAAG CTCCTCCGGC AGGGATACAC CATTCGCATC
    CTGCTCTGGA CATTTGTCCA GTCTTTTAAG GCACTGCCTT ATGTTTGCCT TCTAATCGCC
    ATGCTGTTTT TCATCTACGC CATTATTGGG ATGCAGGTGT TTGGAAACAT TGATTTGAAT
    GATGACACGG CAATCAACCG TCACAACAAC TTCCGCACTT TCCTCCAAGC TCTTATGCTT
    TTGTTCAGGA GTGCCACTGG CGAGGCTTGG CATGATATCA TGCTGTCGTG TCTGAGCGAG
    CGCACCTGTG ATCCGAGCTC AGGCACCCTT GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC
    TTCTACTTTG TCTCCTTCAT TTTCCTCTGC TCATTTCTGA TGTTGAACCT GTTTGTGGCC
    GTCATTATGG ACAACTTTGA ATATCTGACA CGCGACTCCT CCATCCTGGG CCCTCACCAC
    CTGGATGAGT TTATTCGAGT GTGGGCGGAG TATGACCCAG CCGCATGTGG CCGTATCAAG
    TACCTCGATA TGTATGAGAT GCTGCTCCAC ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA
    TGTCCTCCAC GAATCGCCTA CAAGCGTCTG GTGAAAATGA ACATGCCCAT TGCGGATGAC
    AACAGTGTAC ACTTTACCTC AACGCTGATG GCTCTGATCC GTACTGCCCT GGAGATCAAA
    CTGGCTTCAG GCATGGTGGC TCAGCGTCTG TCAGATGCTG AACTGAAGAA GGAGCTCTCT
    ACAGTTTGGC CAAACCTGTC TCAGAAGACC ATGGACCTGC TGGTCACTCC ACATAAACCT
    AATGAGCTCA CAGTGGGGAA GGTGTATGCA GCTCTTATGA TCTTTGACTA TTACAAGCAG
    AACAGAGCAA AAAGACTTCA GATGCAGCAA CAGCAGCTGA AAGAACAGGC AGGACCTGGA
    TCTCAGAATA AAGTCAGCGC TCTCTTAGAG CCCATTCTCC CACTAACCCA CATGCAAGAT
    CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA GAGTGTCAAG CTCAGACCAG ACCCAGCTCC
    ACCACACTCA ACAATGGACG CACAACTCTT GACAACACAA TAAAAGGGTC TTCATCCTGG
    GCCTCAGAGA AATCCAAGGA AGTCCAAAAG TCTAAACGGA GACCAATGTC TAGAGGTCAA
    TCTGAGGATG CCTCAAATGC AAACACTGCC CAGGAGTCAG TAGAAATGAG AAAGATGGAG
    AACAGTGCCA ACACAGTGAC CTCTGGTGGC CTTGAGGGCC AAGGACGTGC AGCGTCCATG
    CCAAAACTCA ATGCTGAGAT GCAGAGGAGT CATTCCCGTC AATCTCCTGG AACATTACTG
    GAACCCATTC CAGATTCCCC CATGAGGCGC TCTGCATCCA CTTTTGCACC ACAGCGCCCT
    CACGAGGTGA ATCTGAATGA CTACATTCTG GAGAAACCTG TTCAGGAACG CCATCACCAC
    CATCACCATC ATCACCGATG CCATCATCGC CGGGACAGAG ACAAGAAACA GAGATCTCTG
    GACAGGTCGC CTACTGGCCA TCACAAAACT ACAGGCACAG CCACTGACTC TGTAGAGGAG
    AGGGCACATG ACCGGGGTCG TTCCCATGAG AGGAAGCACC ATTCATCTTC AGGAGAGAAG
    CAGCGGTATT ACTCGTGTGA TCGCTATGCC AGTCGGGAAC ACTGTTCCAG CAAATCTGCC
    GTGGCAAGCT GTGCCACCTC ACCCAGCGAG ACCCAGGAGG CCAATCTTAA CAAGCAGGGC
    AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG TTGACTTCAG GGGCGAGCAC TCCAAGCCGC
    GGGCGCAGAC AGCTGCCCCA GACCCCCCTC ACTCCTCGTC CCTCCGTGGC CTACAGGACT
    GCCAACTCTT CCCCTGTGCA ACTTCTGGCC ACAACGACCA GCTTGCTTCC CCCGAGTCCC
    GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC AACGCTCTTT TACTCAGCGG CTCTATCTCC
    AGCTCGCCGG CCCCCGTCAG TCGCATTAAC TCAGAACCCT TCTTGGGGCC CCCGGACCAC
    CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT GTCTTCCAAG AGAATCCGAG CCCTCAGATG
    GGCCGTTCTC CTCAGACTGT AGGGATGCCC TTCGTGGCGA TCCCCCCACC TCCCCCACAG
    CAGTTTCGCA GGGTCCCCAA CGGGTACCAC TTCTCCACGG GCCCCAGCGC CAGCAGAGGA
    CGGGCACAGC AGTACTACCC AGAGGCAGAG GTGGAGGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211586.1
    CDS637..7704
    Translation

    Target ORF information:

    RefSeq Version XM_017356097.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356097.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC
    ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA
    CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC
    CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG
    GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG
    TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG
    TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG
    GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT
    CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC
    TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG
    GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT
    GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG
    AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT
    GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT
    CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC
    GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC
    AGCATCACTG ACATTTTAGT CACTGAGATT AACGAGCGTC AGCTGAACTT CAGTTTCCTG
    AGGCTCTTTA GAGCAGCCCG ACTCATCAAG CTCCTCCGGC AGGGATACAC CATTCGCATC
    CTGCTCTGGA CATTTGTCCA GTCTTTTAAG GCACTGCCTT ATGTTTGCCT TCTAATCGCC
    ATGCTGTTTT TCATCTACGC CATTATTGGG ATGCAGGTGT TTGGAAACAT TGATTTGAAT
    GATGACACGG CAATCAACCG TCACAACAAC TTCCGCACTT TCCTCCAAGC TCTTATGCTT
    TTGTTCAGGA GTGCCACTGG CGAGGCTTGG CATGATATCA TGCTGTCGTG TCTGAGCGAG
    CGCACCTGTG ATCCGAGCTC AGGCACCCTT GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC
    TTCTACTTTG TCTCCTTCAT TTTCCTCTGC TCATTTCTGA TGTTGAACCT GTTTGTGGCC
    GTCATTATGG ACAACTTTGA ATATCTGACA CGCGACTCCT CCATCCTGGG CCCTCACCAC
    CTGGATGAGT TTATTCGAGT GTGGGCGGAG TATGACCCAG CCGCATGTGG CCGTATCAAG
    TACCTCGATA TGTATGAGAT GCTGCTCCAC ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA
    TGTCCTCCAC GAATCGCCTA CAAGCGTCTG GTGAAAATGA ACATGCCCAT TGCGGATGAC
    AACAGTGTAC ACTTTACCTC AACGCTGATG GCTCTGATCC GTACTGCCCT GGAGATCAAA
    CTGGCTTCAG GCATGGTGGC TCAGCGTCTG TCAGATGCTG AACTGAAGAA GGAGCTCTCT
    ACAGTTTGGC CAAACCTGTC TCAGAAGACC ATGGACCTGC TGGTCACTCC ACATAAACCT
    AATGAGCTCA CAGTGGGGAA GGTGTATGCA GCTCTTATGA TCTTTGACTA TTACAAGCAG
    AACAGAGCAA AAAGACTTCA GATGCAGCAA CAGCAGCTGA AAGAACAGGC AGGACCTGGA
    TCTCAGAATA AAGTCAGCGC TCTCTTAGAG CCCATTCTCC CACTAACCCA CATGCAAGAT
    CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA GAGTGTCAAG CTCAGACCAG ACCCAGCTCC
    ACCACACTCA ACAATGGACG CACAACTCTT GACAACACAA TAAAAGGGTC TTCATCCTGG
    GCCTCAGAGA AATCCAAGGA AGTCCAAAAG TCTAAACGGA GACCAATGTC TAGAGGTCAA
    TCTGAGGATG CCTCAAATGC AAACACTGCC CAGGAGTCAG TAGAAATGAG AAAGATGGAG
    AACAGTGCCA ACACAGTGAC CTCTGGTGGC CTTGAGGGCC AAGGACGTGC AGCGTCCATG
    CCAAAACTCA ATGCTGAGAT GCAGAGGAGT CATTCCCGTC AATCTCCTGG AACATTACTG
    GAACCCATTC CAGATTCCCC CATGAGGCGC TCTGCATCCA CTTTTGCACC ACAGCGCCCT
    CACGAGGTGA ATCTGAATGA CTACATTCTG GAGAAACCTG TTCAGGAACG CCATCACCAC
    CATCACCATC ATCACCGATG CCATCATCGC CGGGACAGAG ACAAGAAACA GAGATCTCTG
    GACAGGTCGC CTACTGGCCA TCACAAAACT ACAGGCACAG CCACTGACTC TGTAGAGGAG
    AGGGCACATG ACCGGGGTCG TTCCCATGAG AGGAAGCACC ATTCATCTTC AGGAGAGAAG
    CAGCGGTATT ACTCGTGTGA TCGCTATGCC AGTCGGGAAC ACTGTTCCAG CAAATCTGCC
    GTGGCAAGCT GTGCCACCTC ACCCAGCGAG ACCCAGGAGG CCAATCTTAA CAAGCAGGGC
    AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG TTGACTTCAG GGGCGAGCAC TCCAAGCCGC
    GGGCGCAGAC AGCTGCCCCA GACCCCCCTC ACTCCTCGTC CCTCCGTGGC CTACAGGACT
    GCCAACTCTT CCCCTGTGCA ACTTCTGGCC ACAACGACCA GCTTGCTTCC CCCGAGTCCC
    GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC AACGCTCTTT TACTCAGCGG CTCTATCTCC
    AGCTCGCCGG CCCCCGTCAG TCGCATTAAC TCAGAACCCT TCTTGGGGCC CCCGGACCAC
    CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT GTCTTCCAAG AGAATCCGAG CCCTCAGATG
    GGCCGTTCTC CTCAGACTGT AGGGATGCCC TTCGTGGCGA TCCCCCCACC TCCCCCACAG
    CAGTTTCGCA GGGTCCCCAA CGGGTACCAC TTCTCCACGG GCCCCAGCGC CAGCAGAGGA
    CGGGCACAGC AGTACTACCC AGAGGCAGAG GTGGAGGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa36125
    Clone ID Related Accession (Same CDS sequence) NM_001114548.1
    Accession Version NM_001114548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929342.7 and BX571772.8. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGTCCA TTTGAGTACA TGATTCTCGC CACAATCATA
    GCAAACTGCA TTGTGTTGGG CTTAGAACAG CATTTACCAG CGTTAGACAA GACACCCATG
    TCAAAACGGC TGGATGACAC TGAGCCCTAC TTCATCGGAA TATTTTGCTT TGAGGCTGGG
    ATCAAGATCA TCGCTCTAGG TTTTGCTTTT CATAAAGGCT CCTACCTCCG CAATGGCTGG
    AATGTTATGG ATTTTGTTGT CGTGTTGACG GGAATTCTAA CCATAGTTGG GCCAGACTTT
    GATTTACGGA CACTCAGGGC AGTGAGAGTC TTGCGGCCAC TCAAACTCGT GTCTGGGATT
    CCTAGTCTCC AAGTAGTTCT GAAGTCCATC ATGAAAGCGA TGGTTCCTTT GCTTCAGATC
    GGCCTTCTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGTCTAGA CTTTTACATG
    GGCAAGTTTC ACCGCACCTG CTTCAGAACA GATACTGGGG AACAGGTGGA TGAATTTCCA
    TGTGGGCTCG AGTCGCCAGC CTGGACGTGT GAAAACGGCA CTGAATGTAG GGAATACTGG
    ATCGGCCCAA ACTTCGGCAT TACTAACTTT GACAACATCC TCTTCGCTGT GCTCACCGTC
    TTCCAGTGCA TCACTATGGA AGGATGGGTG GACATCCTTT ACAATGCCAA CGATGCCTCA
    GGGAACACCT GGAACTGGCT CTACTTCATC CCACTCATCA TCATCGGCTC CTTCTTCATG
    CTCAACCTGG TTCTGGGTGT GCTGTCAGGG GAGTTTGCAA AGGAGCGAGA GCGTGTGGAG
    AAAAGACAGG AGTTCTTAAA GCTGCGCAGA CAACAGCAGA TCGAAAGAGA GTTAACCGGA
    TACCTGGAGT GGATCTGCAA GGCAGAGGAA GTAATGCTTG CTGAAGAAGA TAAAAATGCA
    GAGGACAAGG ATGATGTTGC CTGGTACAAG AGGAAATGCA ACAACCCAGT TTTGAAGCGG
    GCCAAGAAGA GCAAAAATGA CCTCATCAAT GCAGAGGAAG GAGAAGATCA CTTTACTGAC
    ATCTCTTCAG TTGCTCCCCA AGGCTCTCCA TTCACCCGCA CAAGTGTGAA GAGCAGCAAG
    ATTGAGAGCT TGTCGTACTT TCGCAGAAAA GAAAAGAGGT TTCGCTTCTT CATCCGCCGC
    ATGGTGAAGG CTCAGAGTTT CTACTGGACT GTTCTGTGTA TAGTGGGACT GAACACACTG
    TGTGTGGCCA TAGTCCACTA CGACCAGCCC GAATGGCTCA CATATGCACT GTATTTGGCG
    GAGTTTGTGT TCCTTGGTCT GTTCCTAATA GAGATGAGTC TGAAGATGTA TGGCCTCGGG
    CCCAGGACTT ACTTTCACTC TTCATTTAAC TGCTTTGACT TTGGGGTGAT TGTGGGGAGC
    ATTTTCGAGG TGATCTGGGC TGCAGTAAAG CCAGGAGCTT CCTTTGGCAT TAGTGTGTTG
    CGAGCTCTTC GTTTGCTAAG AATATTCAAG GTTACCAAGT ATTGGAACTC ATTGAGGAAT
    CTGGTAGTGT CTCTGCTCAA CTCAATGAAG TCCATCATTA GTCTCCTGTT TCTGCTGTTC
    CTCTTCATCG TGGTGTTTGC TCTGTTGGGA ATGCAGCTCT TTGGGGGACA GTTTAATTTT
    GAAGACGAGA CCCCGACAAC CAACTTTGAC ACATTCCCAG CTGCTATCTT GACAGTGTTT
    CAGATTTTGA CTGGAGAGGA CTGGAACGCT GTGATGTATC ATGGGATTGA ATCGCAGGGC
    GGAGTGCACC GGGGAATGTT CTGCTCCGTG TACTTCATCG TGCTCACGCT GTTTGGAAAC
    TACACTCTCC TCAATGTCTT TTTGGCCATC GCTGTTGATA ACCTTGCCAA TGCACAAGAA
    CTCACCAAGG ATGAGGAGGA GCAAGAGGAG GCTATCAGTA AGAAACTGGC TCTACAGAAA
    GCCAAGGAGG TAAAGGAGGT CAGCCCCATG TCGGCAGCTA ACATTTCCAT CACAGCTTTT
    ATAAGGCCAA ACCCTGGTGC TCTTTCATGC AGCTCCTCCA ACTCCAGCTC TCATTCACCT
    AAAGAACAGC AGCGTTCTCT AAAAATGATG TCCGTGTGGG AGCAGCGCAC CACTCAGATC
    CGAAAGCACA TGCTAGCCAG CAGTGAGGCG TTGTACCAAG AAGAGCTCAA TCCACGGCTC
    ATGTCTAACC TTCCTCTACG CACCGACATA AAAACCCACC TGGACCGGCC TTTGGTTGTG
    GAGCCTCGCT GTGATGGCCC CATCAGCCCA CACAGTGGCT GGGACAAACC CCAGGATTTG
    CAAGGCGAGG GCAGCACACC AGAAGAACGA GTCCCACCTG TATTCGAGCC TCCACAGTCA
    CATCCACCAC GCAAGCACCA CCGTCACAGA GAGCGTCTTT CTAATGAGAC CAATGAAAAT
    GGAGACACGG GTCATGCCAA AGAAGGCCGG CATCACGTTC ATCACAGCCG AAGTAAAGAG
    CATGATGGGA CACGTTGTAA GGAGGGGAAG GGTGACAGGA GCCGAAGCAG AGAAGGGGGA
    CGTAGACATC ACCATCAGAG CTCGGTGGAT GAGGGCGTAG GAGGTGGAGA GAGGGAACAT
    CGGCACCATC ACTCCCATAG GCATAACAGA GAGGGCAATG GTGTGGTCAG TGCTGGGGGG
    AAGAGTGAAC GGCGGTCCAG GCAGAAAGAG GGCTCACGAT CAGGGACAAG AGAGGGAGAG
    AGATGCTCAA GGGGGGAGAA TGGAGGAAAT GGAGGGAGGA GGCGACACAG GCCACGAACC
    AGTAAAGCTC AGTCTACGCT GGAAGGAGAG GAGCAACATG TGAATGGAGA GGAGGAGGGC
    AAGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGCTCTTCAA GGGCAAGTTT
    TTTTACTGCA CAGACGAGTC CAAGGGCCTG GAGAAAGACT GCAGGGGGCT GTTTTTGGAT
    TATGATAAGG ATGATGTGAC GGCACAACCG AGGGAATGGA AGAAATACGA GTTTCACTAT
    GACAATGTGC TTTGGGCTTT TCTCACTCTC TTCACTGTTT CTACGGGAGA GGGCTGGCCT
    ACGGTTTTGA AGCACTCCGT GGATGCCACT TTTGAGGATC AGGGTCCCAG TCCAGGCTAC
    CGCATTGAGA TGTCTATTTT TTACGTGGTC TACTTTGTGG TTTTCCCTTT CTTCTTCGTC
    AACATTTTTG TAGCCTTGAT AATCATCACA TTCCAGGAAC AGGGTGATAA GGTCTTGTCA
    GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC TGCATCGACT TTGCTATCAA TGCCAAACCT
    CTGACACGCT ACATGCCGCA GAACCAGCAG TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG
    GTCTCTCCGC CGTTTGAGTA CTCCATCATG ATCATGATTG CTCTCAACAC TGTGGTGCTC
    ATGATGAAGT TCCATGGGGC GCCTGACTTT TATGAGGCAA TGCTTAAGTA TCTCAACATT
    GTCTTCACTG TTCTCTTTTC ACTGGAGTGT ATTCTCAAGA TTATTGCTTT CGGCCCCTTG
    AATTACCTGA AAGATGCCTG GAATGTGTTT GACTTTGTGA CTGTGCTGGG CAGCATCACT
    GACATTTTAG TCACTGAGAT TAACGAGCGT CAGCTGAACT TCAGTTTCCT GAGGCTCTTT
    AGAGCAGCCC GACTCATCAA GCTCCTCCGG CAGGGATACA CCATTCGCAT CCTGCTCTGG
    ACATTTGTCC AGTCTTTTAA GGCACTGCCT TATGTTTGCC TTCTAATCGC CATGCTGTTT
    TTCATCTACG CCATTATTGG GATGCAGGTG TTTGGAAACA TTGATTTGAA TGATGACACG
    GCAATCAACC GTCACAACAA CTTCCGCACT TTCCTCCAAG CTCTTATGCT TTTGTTCAGG
    AGTGCCACTG GCGAGGCTTG GCATGATATC ATGCTGTCGT GTCTGAGCGA GCGCACCTGT
    GATCCGAGCT CAGGCACCCT TGGAAAGGAG TGTGGGAGTG ACTTTGCCTA CTTCTACTTT
    GTCTCCTTCA TTTTCCTCTG CTCATTTCTG ATGTTGAACC TGTTTGTGGC CGTCATTATG
    GACAACTTTG AATATCTGAC ACGCGACTCC TCCATCCTGG GCCCTCACCA CCTGGATGAG
    TTTATTCGAG TGTGGGCGGA GTATGACCCA GCCGCATGTG GCCGTATCAA GTACCTCGAT
    ATGTATGAGA TGCTGCTCCA CATGTCCCCA CCTTTAGGTT TGGGAAAGAA ATGTCCTCCA
    CGAATCGCCT ACAAGCGTCT GGTGAAAATG AACATGCCCA TTGCGGATGA CAACAGTGTA
    CACTTTACCT CAACGCTGAT GGCTCTGATC CGTACTGCCC TGGAGATCAA ACTGGCTTCA
    GGCATGGTGG CTCAGCGTCT GTCAGATGCT GAACTGAAGA AGGAGCTCTC TACAGTTTGG
    CCAAACCTGT CTCAGAAGAC CATGGACCTG CTGGTCACTC CACATAAACC TAATGAGCTC
    ACAGTGGGGA AGGTGTATGC AGCTCTTATG ATCTTTGACT ATTACAAGCA GAACAGAGCA
    AAAAGACTTC AGATGCAGCA ACAGCAGCTG AAAGAACAGG CAGGACCTGG ATCTCAGAAT
    AAAGTCAGCG CTCTCTTAGA GCCCATTCTC CCACTAACCC ACATGCAAGA TCAGCCAGTG
    AACGGTGTCG ATTCAGCTTC AGAGTGTCAA GCTCAGACCA GACCCAGCTC CACCACACTC
    AACAATGGAC GCACAACTCT TGACAACACA ATAAAAGGGT CTTCATCCTG GGCCTCAGAG
    AAATCCAAGG AAGTCCAAAA GTCTAAACGG AGACCAATGT CTAGAGGTCA ATCTGAGGAT
    GCCTCAAATG CAAACACTGC CCAGGAGTCA GTAGAAATGA GAAAGATGGA GAACAGTGCC
    AACACAGTGA CCTCTGGTGG CCTTGAGGGC CAAGGACGTG CAGCGTCCAT GCCAAAACTC
    AATGCTGAGA TGCAGAGGAG TCATTCCCGT CAATCTCCTG GAACATTACT GGAACCCATT
    CCAGATTCCC CCATGAGGCG CTCTGCATCC ACTTTTGCAC CACAGCGCCC TCACGAGGTG
    AATCTGAATG ACTACATTCT GGAGAAACCT GTTCAGGAAC GCCATCACCA CCATCACCAT
    CATCACCGAT GCCATCATCG CCGGGACAGA GACAAGAAAC AGAGATCTCT GGACAGGTCG
    CCTACTGGCC ATCACAAAAC TACAGGCACA GCCACTGACT CTGTAGAGGA GAGGGCACAT
    GACCGGGGTC GTTCCCATGA GAGGAAGCAC CATTCATCTT CAGGAGAGAA GCAGCGGTAT
    TACTCGTGTG ATCGCTATGC CAGTCGGGAA CACTGTTCCA GCAAATCTGC CGTGGCAAGC
    TGTGCCACCT CACCCAGCGA GACCCAGGAG GCCAATCTTA ACAAGCAGGG CAGTGGGTGG
    GCTAAGGGGA GCCCGGTGTT GTTGACTTCA GGGGCGAGCA CTCCAAGCCG CGGGCGCAGA
    CAGCTGCCCC AGACCCCCCT CACTCCTCGT CCCTCCGTGG CCTACAGGAC TGCCAACTCT
    TCCCCTGTGC AACTTCTGGC CACAACGACC AGCTTGCTTC CCCCGAGTCC CGGCAGGCTG
    AGCCGTGGTC TGTCCGAGCA CAACGCTCTT TTACTCAGCG GCTCTATCTC CAGCTCGCCG
    GCCCCCGTCA GTCGCATTAA CTCAGAACCC TTCTTGGGGC CCCCGGACCA CCCTGCTGGC
    TTGCAGCCCG AAGCCTTCGC TGTCTTCCAA GAGAATCCGA GCCCTCAGAT GGGCCGTTCT
    CCTCAGACTG TAGGGATGCC CTTCGTGGCG ATCCCCCCAC CTCCCCCACA GCAGTTTCGC
    AGGGTCCCCA ACGGGTACCA CTTCTCCACG GGCCCCAGCG CCAGCAGAGG ACGGGCACAG
    CAGTACTACC CAGAGGCAGA GGTGGAGGAC TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001108020.1
    CDS1..6939
    Translation

    Target ORF information:

    RefSeq Version NM_001114548.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001114548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGTCCA TTTGAGTACA TGATTCTCGC CACAATCATA
    GCAAACTGCA TTGTGTTGGG CTTAGAACAG CATTTACCAG CGTTAGACAA GACACCCATG
    TCAAAACGGC TGGATGACAC TGAGCCCTAC TTCATCGGAA TATTTTGCTT TGAGGCTGGG
    ATCAAGATCA TCGCTCTAGG TTTTGCTTTT CATAAAGGCT CCTACCTCCG CAATGGCTGG
    AATGTTATGG ATTTTGTTGT CGTGTTGACG GGAATTCTAA CCATAGTTGG GCCAGACTTT
    GATTTACGGA CACTCAGGGC AGTGAGAGTC TTGCGGCCAC TCAAACTCGT GTCTGGGATT
    CCTAGTCTCC AAGTAGTTCT GAAGTCCATC ATGAAAGCGA TGGTTCCTTT GCTTCAGATC
    GGCCTTCTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGTCTAGA CTTTTACATG
    GGCAAGTTTC ACCGCACCTG CTTCAGAACA GATACTGGGG AACAGGTGGA TGAATTTCCA
    TGTGGGCTCG AGTCGCCAGC CTGGACGTGT GAAAACGGCA CTGAATGTAG GGAATACTGG
    ATCGGCCCAA ACTTCGGCAT TACTAACTTT GACAACATCC TCTTCGCTGT GCTCACCGTC
    TTCCAGTGCA TCACTATGGA AGGATGGGTG GACATCCTTT ACAATGCCAA CGATGCCTCA
    GGGAACACCT GGAACTGGCT CTACTTCATC CCACTCATCA TCATCGGCTC CTTCTTCATG
    CTCAACCTGG TTCTGGGTGT GCTGTCAGGG GAGTTTGCAA AGGAGCGAGA GCGTGTGGAG
    AAAAGACAGG AGTTCTTAAA GCTGCGCAGA CAACAGCAGA TCGAAAGAGA GTTAACCGGA
    TACCTGGAGT GGATCTGCAA GGCAGAGGAA GTAATGCTTG CTGAAGAAGA TAAAAATGCA
    GAGGACAAGG ATGATGTTGC CTGGTACAAG AGGAAATGCA ACAACCCAGT TTTGAAGCGG
    GCCAAGAAGA GCAAAAATGA CCTCATCAAT GCAGAGGAAG GAGAAGATCA CTTTACTGAC
    ATCTCTTCAG TTGCTCCCCA AGGCTCTCCA TTCACCCGCA CAAGTGTGAA GAGCAGCAAG
    ATTGAGAGCT TGTCGTACTT TCGCAGAAAA GAAAAGAGGT TTCGCTTCTT CATCCGCCGC
    ATGGTGAAGG CTCAGAGTTT CTACTGGACT GTTCTGTGTA TAGTGGGACT GAACACACTG
    TGTGTGGCCA TAGTCCACTA CGACCAGCCC GAATGGCTCA CATATGCACT GTATTTGGCG
    GAGTTTGTGT TCCTTGGTCT GTTCCTAATA GAGATGAGTC TGAAGATGTA TGGCCTCGGG
    CCCAGGACTT ACTTTCACTC TTCATTTAAC TGCTTTGACT TTGGGGTGAT TGTGGGGAGC
    ATTTTCGAGG TGATCTGGGC TGCAGTAAAG CCAGGAGCTT CCTTTGGCAT TAGTGTGTTG
    CGAGCTCTTC GTTTGCTAAG AATATTCAAG GTTACCAAGT ATTGGAACTC ATTGAGGAAT
    CTGGTAGTGT CTCTGCTCAA CTCAATGAAG TCCATCATTA GTCTCCTGTT TCTGCTGTTC
    CTCTTCATCG TGGTGTTTGC TCTGTTGGGA ATGCAGCTCT TTGGGGGACA GTTTAATTTT
    GAAGACGAGA CCCCGACAAC CAACTTTGAC ACATTCCCAG CTGCTATCTT GACAGTGTTT
    CAGATTTTGA CTGGAGAGGA CTGGAACGCT GTGATGTATC ATGGGATTGA ATCGCAGGGC
    GGAGTGCACC GGGGAATGTT CTGCTCCGTG TACTTCATCG TGCTCACGCT GTTTGGAAAC
    TACACTCTCC TCAATGTCTT TTTGGCCATC GCTGTTGATA ACCTTGCCAA TGCACAAGAA
    CTCACCAAGG ATGAGGAGGA GCAAGAGGAG GCTATCAGTA AGAAACTGGC TCTACAGAAA
    GCCAAGGAGG TAAAGGAGGT CAGCCCCATG TCGGCAGCTA ACATTTCCAT CACAGCTTTT
    ATAAGGCCAA ACCCTGGTGC TCTTTCATGC AGCTCCTCCA ACTCCAGCTC TCATTCACCT
    AAAGAACAGC AGCGTTCTCT AAAAATGATG TCCGTGTGGG AGCAGCGCAC CACTCAGATC
    CGAAAGCACA TGCTAGCCAG CAGTGAGGCG TTGTACCAAG AAGAGCTCAA TCCACGGCTC
    ATGTCTAACC TTCCTCTACG CACCGACATA AAAACCCACC TGGACCGGCC TTTGGTTGTG
    GAGCCTCGCT GTGATGGCCC CATCAGCCCA CACAGTGGCT GGGACAAACC CCAGGATTTG
    CAAGGCGAGG GCAGCACACC AGAAGAACGA GTCCCACCTG TATTCGAGCC TCCACAGTCA
    CATCCACCAC GCAAGCACCA CCGTCACAGA GAGCGTCTTT CTAATGAGAC CAATGAAAAT
    GGAGACACGG GTCATGCCAA AGAAGGCCGG CATCACGTTC ATCACAGCCG AAGTAAAGAG
    CATGATGGGA CACGTTGTAA GGAGGGGAAG GGTGACAGGA GCCGAAGCAG AGAAGGGGGA
    CGTAGACATC ACCATCAGAG CTCGGTGGAT GAGGGCGTAG GAGGTGGAGA GAGGGAACAT
    CGGCACCATC ACTCCCATAG GCATAACAGA GAGGGCAATG GTGTGGTCAG TGCTGGGGGG
    AAGAGTGAAC GGCGGTCCAG GCAGAAAGAG GGCTCACGAT CAGGGACAAG AGAGGGAGAG
    AGATGCTCAA GGGGGGAGAA TGGAGGAAAT GGAGGGAGGA GGCGACACAG GCCACGAACC
    AGTAAAGCTC AGTCTACGCT GGAAGGAGAG GAGCAACATG TGAATGGAGA GGAGGAGGGC
    AAGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGCTCTTCAA GGGCAAGTTT
    TTTTACTGCA CAGACGAGTC CAAGGGCCTG GAGAAAGACT GCAGGGGGCT GTTTTTGGAT
    TATGATAAGG ATGATGTGAC GGCACAACCG AGGGAATGGA AGAAATACGA GTTTCACTAT
    GACAATGTGC TTTGGGCTTT TCTCACTCTC TTCACTGTTT CTACGGGAGA GGGCTGGCCT
    ACGGTTTTGA AGCACTCCGT GGATGCCACT TTTGAGGATC AGGGTCCCAG TCCAGGCTAC
    CGCATTGAGA TGTCTATTTT TTACGTGGTC TACTTTGTGG TTTTCCCTTT CTTCTTCGTC
    AACATTTTTG TAGCCTTGAT AATCATCACA TTCCAGGAAC AGGGTGATAA GGTCTTGTCA
    GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC TGCATCGACT TTGCTATCAA TGCCAAACCT
    CTGACACGCT ACATGCCGCA GAACCAGCAG TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG
    GTCTCTCCGC CGTTTGAGTA CTCCATCATG ATCATGATTG CTCTCAACAC TGTGGTGCTC
    ATGATGAAGT TCCATGGGGC GCCTGACTTT TATGAGGCAA TGCTTAAGTA TCTCAACATT
    GTCTTCACTG TTCTCTTTTC ACTGGAGTGT ATTCTCAAGA TTATTGCTTT CGGCCCCTTG
    AATTACCTGA AAGATGCCTG GAATGTGTTT GACTTTGTGA CTGTGCTGGG CAGCATCACT
    GACATTTTAG TCACTGAGAT TAACGAGCGT CAGCTGAACT TCAGTTTCCT GAGGCTCTTT
    AGAGCAGCCC GACTCATCAA GCTCCTCCGG CAGGGATACA CCATTCGCAT CCTGCTCTGG
    ACATTTGTCC AGTCTTTTAA GGCACTGCCT TATGTTTGCC TTCTAATCGC CATGCTGTTT
    TTCATCTACG CCATTATTGG GATGCAGGTG TTTGGAAACA TTGATTTGAA TGATGACACG
    GCAATCAACC GTCACAACAA CTTCCGCACT TTCCTCCAAG CTCTTATGCT TTTGTTCAGG
    AGTGCCACTG GCGAGGCTTG GCATGATATC ATGCTGTCGT GTCTGAGCGA GCGCACCTGT
    GATCCGAGCT CAGGCACCCT TGGAAAGGAG TGTGGGAGTG ACTTTGCCTA CTTCTACTTT
    GTCTCCTTCA TTTTCCTCTG CTCATTTCTG ATGTTGAACC TGTTTGTGGC CGTCATTATG
    GACAACTTTG AATATCTGAC ACGCGACTCC TCCATCCTGG GCCCTCACCA CCTGGATGAG
    TTTATTCGAG TGTGGGCGGA GTATGACCCA GCCGCATGTG GCCGTATCAA GTACCTCGAT
    ATGTATGAGA TGCTGCTCCA CATGTCCCCA CCTTTAGGTT TGGGAAAGAA ATGTCCTCCA
    CGAATCGCCT ACAAGCGTCT GGTGAAAATG AACATGCCCA TTGCGGATGA CAACAGTGTA
    CACTTTACCT CAACGCTGAT GGCTCTGATC CGTACTGCCC TGGAGATCAA ACTGGCTTCA
    GGCATGGTGG CTCAGCGTCT GTCAGATGCT GAACTGAAGA AGGAGCTCTC TACAGTTTGG
    CCAAACCTGT CTCAGAAGAC CATGGACCTG CTGGTCACTC CACATAAACC TAATGAGCTC
    ACAGTGGGGA AGGTGTATGC AGCTCTTATG ATCTTTGACT ATTACAAGCA GAACAGAGCA
    AAAAGACTTC AGATGCAGCA ACAGCAGCTG AAAGAACAGG CAGGACCTGG ATCTCAGAAT
    AAAGTCAGCG CTCTCTTAGA GCCCATTCTC CCACTAACCC ACATGCAAGA TCAGCCAGTG
    AACGGTGTCG ATTCAGCTTC AGAGTGTCAA GCTCAGACCA GACCCAGCTC CACCACACTC
    AACAATGGAC GCACAACTCT TGACAACACA ATAAAAGGGT CTTCATCCTG GGCCTCAGAG
    AAATCCAAGG AAGTCCAAAA GTCTAAACGG AGACCAATGT CTAGAGGTCA ATCTGAGGAT
    GCCTCAAATG CAAACACTGC CCAGGAGTCA GTAGAAATGA GAAAGATGGA GAACAGTGCC
    AACACAGTGA CCTCTGGTGG CCTTGAGGGC CAAGGACGTG CAGCGTCCAT GCCAAAACTC
    AATGCTGAGA TGCAGAGGAG TCATTCCCGT CAATCTCCTG GAACATTACT GGAACCCATT
    CCAGATTCCC CCATGAGGCG CTCTGCATCC ACTTTTGCAC CACAGCGCCC TCACGAGGTG
    AATCTGAATG ACTACATTCT GGAGAAACCT GTTCAGGAAC GCCATCACCA CCATCACCAT
    CATCACCGAT GCCATCATCG CCGGGACAGA GACAAGAAAC AGAGATCTCT GGACAGGTCG
    CCTACTGGCC ATCACAAAAC TACAGGCACA GCCACTGACT CTGTAGAGGA GAGGGCACAT
    GACCGGGGTC GTTCCCATGA GAGGAAGCAC CATTCATCTT CAGGAGAGAA GCAGCGGTAT
    TACTCGTGTG ATCGCTATGC CAGTCGGGAA CACTGTTCCA GCAAATCTGC CGTGGCAAGC
    TGTGCCACCT CACCCAGCGA GACCCAGGAG GCCAATCTTA ACAAGCAGGG CAGTGGGTGG
    GCTAAGGGGA GCCCGGTGTT GTTGACTTCA GGGGCGAGCA CTCCAAGCCG CGGGCGCAGA
    CAGCTGCCCC AGACCCCCCT CACTCCTCGT CCCTCCGTGG CCTACAGGAC TGCCAACTCT
    TCCCCTGTGC AACTTCTGGC CACAACGACC AGCTTGCTTC CCCCGAGTCC CGGCAGGCTG
    AGCCGTGGTC TGTCCGAGCA CAACGCTCTT TTACTCAGCG GCTCTATCTC CAGCTCGCCG
    GCCCCCGTCA GTCGCATTAA CTCAGAACCC TTCTTGGGGC CCCCGGACCA CCCTGCTGGC
    TTGCAGCCCG AAGCCTTCGC TGTCTTCCAA GAGAATCCGA GCCCTCAGAT GGGCCGTTCT
    CCTCAGACTG TAGGGATGCC CTTCGTGGCG ATCCCCCCAC CTCCCCCACA GCAGTTTCGC
    AGGGTCCCCA ACGGGTACCA CTTCTCCACG GGCCCCAGCG CCAGCAGAGG ACGGGCACAG
    CAGTACTACC CAGAGGCAGA GGTGGAGGAC TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42256
    Clone ID Related Accession (Same CDS sequence) XM_009301492.2
    Accession Version XM_009301492.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT
    CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG
    TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT
    GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC
    ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA
    GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT
    GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT
    TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG
    CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG
    TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA
    GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG
    GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC
    ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG
    TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC
    CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT
    CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA
    GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC
    ACTGAGATTA ACACAACGGA GCGTCAGCTG AACTTCAGTT TCCTGAGGCT CTTTAGAGCA
    GCCCGACTCA TCAAGCTCCT CCGGCAGGGA TACACCATTC GCATCCTGCT CTGGACATTT
    GTCCAGTCTT TTAAGGCACT GCCTTATGTT TGCCTTCTAA TCGCCATGCT GTTTTTCATC
    TACGCCATTA TTGGGATGCA GGTGTTTGGA AACATTGATT TGAATGATGA CACGGCAATC
    AACCGTCACA ACAACTTCCG CACTTTCCTC CAAGCTCTTA TGCTTTTGTT CAGGAGTGCC
    ACTGGCGAGG CTTGGCATGA TATCATGCTG TCGTGTCTGA GCGAGCGCAC CTGTGATCCG
    AGCTCAGGCA CCCTTGGAAA GGAGTGTGGG AGTGACTTTG CCTACTTCTA CTTTGTCTCC
    TTCATTTTCC TCTGCTCATT TCTGATGTTG AACCTGTTTG TGGCCGTCAT TATGGACAAC
    TTTGAATATC TGACACGCGA CTCCTCCATC CTGGGCCCTC ACCACCTGGA TGAGTTTATT
    CGAGTGTGGG CGGAGTATGA CCCAGCCGCA TGTGGCCGTA TCAAGTACCT CGATATGTAT
    GAGATGCTGC TCCACATGTC CCCACCTTTA GGTTTGGGAA AGAAATGTCC TCCACGAATC
    GCCTACAAGC GTCTGGTGAA AATGAACATG CCCATTGCGG ATGACAACAG TGTACACTTT
    ACCTCAACGC TGATGGCTCT GATCCGTACT GCCCTGGAGA TCAAACTGGC TTCAGGCATG
    GTGGCTCAGC GTCTGTCAGA TGCTGAACTG AAGAAGGAGC TCTCTACAGT TTGGCCAAAC
    CTGTCTCAGA AGACCATGGA CCTGCTGGTC ACTCCACATA AACCTAATGA GCTCACAGTG
    GGGAAGGTGT ATGCAGCTCT TATGATCTTT GACTATTACA AGCAGAACAG AGCAAAAAGA
    CTTCAGATGC AGCAACAGCA GCTGAAAGAA CAGGCAGGAC CTGGATCTCA GAATAAAGTC
    AGCGCTCTCT TAGAGCCCAT TCTCCCACTA ACCCACATGC AAGATCAGCC AGTGAACGGT
    GTCGATTCAG CTTCAGAGTG TCAAGCTCAG ACCAGACCCA GCTCCACCAC ACTCAACAAT
    GGACGCACAA CTCTTGACAA CACAATAAAA GGGTCTTCAT CCTGGGCCTC AGAGAAATCC
    AAGGAAGTCC AAAAGTCTAA ACGGAGACCA ATGTCTAGAG GTCAATCTGA GGATGCCTCA
    AATGCAAACA CTGCCCAGGA GTCAGTAGAA ATGAGAAAGA TGGAGAACAG TGCCAACACA
    GTGACCTCTG GTGGCCTTGA GGGCCAAGGA CGTGCAGCGT CCATGCCAAA ACTCAATGCT
    GAGATGCAGA GGAGTCATTC CCGTCAATCT CCTGGAACAT TACTGGAACC CATTCCAGAT
    TCCCCCATGA GGCGCTCTGC ATCCACTTTT GCACCACAGC GCCCTCACGA GGTGAATCTG
    AATGACTACA TTCTGGAGAA ACCTGTTCAG GAACGCCATC ACCACCATCA CCATCATCAC
    CGATGCCATC ATCGCCGGGA CAGAGACAAG AAACAGAGAT CTCTGGACAG GTCGCCTACT
    GGCCATCACA AAACTACAGG CACAGCCACT GACTCTGTAG AGGAGAGGGC ACATGACCGG
    GGTCGTTCCC ATGAGAGGAA GCACCATTCA TCTTCAGGAG AGAAGCAGCG GTATTACTCG
    TGTGATCGCT ATGCCAGTCG GGAACACTGT TCCAGCAAAT CTGCCGTGGC AAGCTGTGCC
    ACCTCACCCA GCGAGACCCA GGAGGCCAAT CTTAACAAGC AGGGCAGTGG GTGGGCTAAG
    GGGAGCCCGG TGTTGTTGAC TTCAGGGGCG AGCACTCCAA GCCGCGGGCG CAGACAGCTG
    CCCCAGACCC CCCTCACTCC TCGTCCCTCC GTGGCCTACA GGACTGCCAA CTCTTCCCCT
    GTGCAACTTC TGGCCACAAC GACCAGCTTG CTTCCCCCGA GTCCCGGCAG GCTGAGCCGT
    GGTCTGTCCG AGCACAACGC TCTTTTACTC AGCGGCTCTA TCTCCAGCTC GCCGGCCCCC
    GTCAGTCGCA TTAACTCAGA ACCCTTCTTG GGGCCCCCGG ACCACCCTGC TGGCTTGCAG
    CCCGAAGCCT TCGCTGTCTT CCAAGAGAAT CCGAGCCCTC AGATGGGCCG TTCTCCTCAG
    ACTGTAGGGA TGCCCTTCGT GGCGATCCCC CCACCTCCCC CACAGCAGTT TCGCAGGGTC
    CCCAACGGGT ACCACTTCTC CACGGGCCCC AGCGCCAGCA GAGGACGGGC ACAGCAGTAC
    TACCCAGAGG CAGAGGTGGA GGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299767.1
    CDS636..7808
    Translation

    Target ORF information:

    RefSeq Version XM_009301492.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT
    CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG
    TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT
    GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC
    ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA
    GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT
    GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT
    TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG
    CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG
    TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA
    GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG
    GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC
    ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG
    TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC
    CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT
    CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA
    GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC
    ACTGAGATTA ACACAACGGA GCGTCAGCTG AACTTCAGTT TCCTGAGGCT CTTTAGAGCA
    GCCCGACTCA TCAAGCTCCT CCGGCAGGGA TACACCATTC GCATCCTGCT CTGGACATTT
    GTCCAGTCTT TTAAGGCACT GCCTTATGTT TGCCTTCTAA TCGCCATGCT GTTTTTCATC
    TACGCCATTA TTGGGATGCA GGTGTTTGGA AACATTGATT TGAATGATGA CACGGCAATC
    AACCGTCACA ACAACTTCCG CACTTTCCTC CAAGCTCTTA TGCTTTTGTT CAGGAGTGCC
    ACTGGCGAGG CTTGGCATGA TATCATGCTG TCGTGTCTGA GCGAGCGCAC CTGTGATCCG
    AGCTCAGGCA CCCTTGGAAA GGAGTGTGGG AGTGACTTTG CCTACTTCTA CTTTGTCTCC
    TTCATTTTCC TCTGCTCATT TCTGATGTTG AACCTGTTTG TGGCCGTCAT TATGGACAAC
    TTTGAATATC TGACACGCGA CTCCTCCATC CTGGGCCCTC ACCACCTGGA TGAGTTTATT
    CGAGTGTGGG CGGAGTATGA CCCAGCCGCA TGTGGCCGTA TCAAGTACCT CGATATGTAT
    GAGATGCTGC TCCACATGTC CCCACCTTTA GGTTTGGGAA AGAAATGTCC TCCACGAATC
    GCCTACAAGC GTCTGGTGAA AATGAACATG CCCATTGCGG ATGACAACAG TGTACACTTT
    ACCTCAACGC TGATGGCTCT GATCCGTACT GCCCTGGAGA TCAAACTGGC TTCAGGCATG
    GTGGCTCAGC GTCTGTCAGA TGCTGAACTG AAGAAGGAGC TCTCTACAGT TTGGCCAAAC
    CTGTCTCAGA AGACCATGGA CCTGCTGGTC ACTCCACATA AACCTAATGA GCTCACAGTG
    GGGAAGGTGT ATGCAGCTCT TATGATCTTT GACTATTACA AGCAGAACAG AGCAAAAAGA
    CTTCAGATGC AGCAACAGCA GCTGAAAGAA CAGGCAGGAC CTGGATCTCA GAATAAAGTC
    AGCGCTCTCT TAGAGCCCAT TCTCCCACTA ACCCACATGC AAGATCAGCC AGTGAACGGT
    GTCGATTCAG CTTCAGAGTG TCAAGCTCAG ACCAGACCCA GCTCCACCAC ACTCAACAAT
    GGACGCACAA CTCTTGACAA CACAATAAAA GGGTCTTCAT CCTGGGCCTC AGAGAAATCC
    AAGGAAGTCC AAAAGTCTAA ACGGAGACCA ATGTCTAGAG GTCAATCTGA GGATGCCTCA
    AATGCAAACA CTGCCCAGGA GTCAGTAGAA ATGAGAAAGA TGGAGAACAG TGCCAACACA
    GTGACCTCTG GTGGCCTTGA GGGCCAAGGA CGTGCAGCGT CCATGCCAAA ACTCAATGCT
    GAGATGCAGA GGAGTCATTC CCGTCAATCT CCTGGAACAT TACTGGAACC CATTCCAGAT
    TCCCCCATGA GGCGCTCTGC ATCCACTTTT GCACCACAGC GCCCTCACGA GGTGAATCTG
    AATGACTACA TTCTGGAGAA ACCTGTTCAG GAACGCCATC ACCACCATCA CCATCATCAC
    CGATGCCATC ATCGCCGGGA CAGAGACAAG AAACAGAGAT CTCTGGACAG GTCGCCTACT
    GGCCATCACA AAACTACAGG CACAGCCACT GACTCTGTAG AGGAGAGGGC ACATGACCGG
    GGTCGTTCCC ATGAGAGGAA GCACCATTCA TCTTCAGGAG AGAAGCAGCG GTATTACTCG
    TGTGATCGCT ATGCCAGTCG GGAACACTGT TCCAGCAAAT CTGCCGTGGC AAGCTGTGCC
    ACCTCACCCA GCGAGACCCA GGAGGCCAAT CTTAACAAGC AGGGCAGTGG GTGGGCTAAG
    GGGAGCCCGG TGTTGTTGAC TTCAGGGGCG AGCACTCCAA GCCGCGGGCG CAGACAGCTG
    CCCCAGACCC CCCTCACTCC TCGTCCCTCC GTGGCCTACA GGACTGCCAA CTCTTCCCCT
    GTGCAACTTC TGGCCACAAC GACCAGCTTG CTTCCCCCGA GTCCCGGCAG GCTGAGCCGT
    GGTCTGTCCG AGCACAACGC TCTTTTACTC AGCGGCTCTA TCTCCAGCTC GCCGGCCCCC
    GTCAGTCGCA TTAACTCAGA ACCCTTCTTG GGGCCCCCGG ACCACCCTGC TGGCTTGCAG
    CCCGAAGCCT TCGCTGTCTT CCAAGAGAAT CCGAGCCCTC AGATGGGCCG TTCTCCTCAG
    ACTGTAGGGA TGCCCTTCGT GGCGATCCCC CCACCTCCCC CACAGCAGTT TCGCAGGGTC
    CCCAACGGGT ACCACTTCTC CACGGGCCCC AGCGCCAGCA GAGGACGGGC ACAGCAGTAC
    TACCCAGAGG CAGAGGTGGA GGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42257
    Clone ID Related Accession (Same CDS sequence) XM_009301493.2
    Accession Version XM_009301493.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT
    CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG
    TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT
    GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC
    ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA
    GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT
    GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT
    TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG
    CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG
    TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA
    GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG
    GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC
    ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG
    TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC
    CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT
    CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA
    GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC
    ACTGAGATTA ACGAGCGTCA GCTGAACTTC AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA
    CTCATCAAGC TCCTCCGGCA GGGATACACC ATTCGCATCC TGCTCTGGAC ATTTGTCCAG
    TCTTTTAAGG CACTGCCTTA TGTTTGCCTT CTAATCGCCA TGCTGTTTTT CATCTACGCC
    ATTATTGGGA TGCAGGTGTT TGGAAACATT GATTTGAATG ATGACACGGC AATCAACCGT
    CACAACAACT TCCGCACTTT CCTCCAAGCT CTTATGCTTT TGTTCAGGAG TGCCACTGGC
    GAGGCTTGGC ATGATATCAT GCTGTCGTGT CTGAGCGAGC GCACCTGTGA TCCGAGCTCA
    GGCACCCTTG GAAAGGAGTG TGGGAGTGAC TTTGCCTACT TCTACTTTGT CTCCTTCATT
    TTCCTCTGCT CATTTCTGAT GTTGAACCTG TTTGTGGCCG TCATTATGGA CAACTTTGAA
    TATCTGACAC GCGACTCCTC CATCCTGGGC CCTCACCACC TGGATGAGTT TATTCGAGTG
    TGGGCGGAGT ATGACCCAGC CGCATGTGGC CGTATCAAGT ACCTCGATAT GTATGAGATG
    CTGCTCCACA TGTCCCCACC TTTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC
    AAGCGTCTGG TGAAAATGAA CATGCCCATT GCGGATGACA ACAGTGTACA CTTTACCTCA
    ACGCTGATGG CTCTGATCCG TACTGCCCTG GAGATCAAAC TGGCTTCAGG CATGGTGGCT
    CAGCGTCTGT CAGATGCTGA ACTGAAGAAG GAGCTCTCTA CAGTTTGGCC AAACCTGTCT
    CAGAAGACCA TGGACCTGCT GGTCACTCCA CATAAACCTA ATGAGCTCAC AGTGGGGAAG
    GTGTATGCAG CTCTTATGAT CTTTGACTAT TACAAGCAGA ACAGAGCAAA AAGACTTCAG
    ATGCAGCAAC AGCAGCTGAA AGAACAGGCA GGACCTGGAT CTCAGAATAA AGTCAGCGCT
    CTCTTAGAGC CCATTCTCCC ACTAACCCAC ATGCAAGATC AGCCAGTGAA CGGTGTCGAT
    TCAGCTTCAG AGTGTCAAGC TCAGACCAGA CCCAGCTCCA CCACACTCAA CAATGGACGC
    ACAACTCTTG ACAACACAAT AAAAGGGTCT TCATCCTGGG CCTCAGAGAA ATCCAAGGAA
    GTCCAAAAGT CTAAACGGAG ACCAATGTCT AGAGGTCAAT CTGAGGATGC CTCAAATGCA
    AACACTGCCC AGGAGTCAGT AGAAATGAGA AAGATGGAGA ACAGTGCCAA CACAGTGACC
    TCTGGTGGCC TTGAGGGCCA AGGACGTGCA GCGTCCATGC CAAAACTCAA TGCTGAGATG
    CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA ACATTACTGG AACCCATTCC AGATTCCCCC
    ATGAGGCGCT CTGCATCCAC TTTTGCACCA CAGCGCCCTC ACGAGGTGAA TCTGAATGAC
    TACATTCTGG AGAAACCTGT TCAGGAACGC CATCACCACC ATCACCATCA TCACCGATGC
    CATCATCGCC GGGACAGAGA CAAGAAACAG AGATCTCTGG ACAGGTCGCC TACTGGCCAT
    CACAAAACTA CAGGCACAGC CACTGACTCT GTAGAGGAGA GGGCACATGA CCGGGGTCGT
    TCCCATGAGA GGAAGCACCA TTCATCTTCA GGAGAGAAGC AGCGGTATTA CTCGTGTGAT
    CGCTATGCCA GTCGGGAACA CTGTTCCAGC AAATCTGCCG TGGCAAGCTG TGCCACCTCA
    CCCAGCGAGA CCCAGGAGGC CAATCTTAAC AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC
    CCGGTGTTGT TGACTTCAGG GGCGAGCACT CCAAGCCGCG GGCGCAGACA GCTGCCCCAG
    ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC TACAGGACTG CCAACTCTTC CCCTGTGCAA
    CTTCTGGCCA CAACGACCAG CTTGCTTCCC CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG
    TCCGAGCACA ACGCTCTTTT ACTCAGCGGC TCTATCTCCA GCTCGCCGGC CCCCGTCAGT
    CGCATTAACT CAGAACCCTT CTTGGGGCCC CCGGACCACC CTGCTGGCTT GCAGCCCGAA
    GCCTTCGCTG TCTTCCAAGA GAATCCGAGC CCTCAGATGG GCCGTTCTCC TCAGACTGTA
    GGGATGCCCT TCGTGGCGAT CCCCCCACCT CCCCCACAGC AGTTTCGCAG GGTCCCCAAC
    GGGTACCACT TCTCCACGGG CCCCAGCGCC AGCAGAGGAC GGGCACAGCA GTACTACCCA
    GAGGCAGAGG TGGAGGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299768.1
    CDS637..7803
    Translation

    Target ORF information:

    RefSeq Version XM_009301493.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301493.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT
    CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG
    TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT
    GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC
    ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA
    GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT
    GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT
    TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG
    CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG
    TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA
    GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG
    GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC
    ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG
    TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC
    CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT
    CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA
    GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC
    ACTGAGATTA ACGAGCGTCA GCTGAACTTC AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA
    CTCATCAAGC TCCTCCGGCA GGGATACACC ATTCGCATCC TGCTCTGGAC ATTTGTCCAG
    TCTTTTAAGG CACTGCCTTA TGTTTGCCTT CTAATCGCCA TGCTGTTTTT CATCTACGCC
    ATTATTGGGA TGCAGGTGTT TGGAAACATT GATTTGAATG ATGACACGGC AATCAACCGT
    CACAACAACT TCCGCACTTT CCTCCAAGCT CTTATGCTTT TGTTCAGGAG TGCCACTGGC
    GAGGCTTGGC ATGATATCAT GCTGTCGTGT CTGAGCGAGC GCACCTGTGA TCCGAGCTCA
    GGCACCCTTG GAAAGGAGTG TGGGAGTGAC TTTGCCTACT TCTACTTTGT CTCCTTCATT
    TTCCTCTGCT CATTTCTGAT GTTGAACCTG TTTGTGGCCG TCATTATGGA CAACTTTGAA
    TATCTGACAC GCGACTCCTC CATCCTGGGC CCTCACCACC TGGATGAGTT TATTCGAGTG
    TGGGCGGAGT ATGACCCAGC CGCATGTGGC CGTATCAAGT ACCTCGATAT GTATGAGATG
    CTGCTCCACA TGTCCCCACC TTTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC
    AAGCGTCTGG TGAAAATGAA CATGCCCATT GCGGATGACA ACAGTGTACA CTTTACCTCA
    ACGCTGATGG CTCTGATCCG TACTGCCCTG GAGATCAAAC TGGCTTCAGG CATGGTGGCT
    CAGCGTCTGT CAGATGCTGA ACTGAAGAAG GAGCTCTCTA CAGTTTGGCC AAACCTGTCT
    CAGAAGACCA TGGACCTGCT GGTCACTCCA CATAAACCTA ATGAGCTCAC AGTGGGGAAG
    GTGTATGCAG CTCTTATGAT CTTTGACTAT TACAAGCAGA ACAGAGCAAA AAGACTTCAG
    ATGCAGCAAC AGCAGCTGAA AGAACAGGCA GGACCTGGAT CTCAGAATAA AGTCAGCGCT
    CTCTTAGAGC CCATTCTCCC ACTAACCCAC ATGCAAGATC AGCCAGTGAA CGGTGTCGAT
    TCAGCTTCAG AGTGTCAAGC TCAGACCAGA CCCAGCTCCA CCACACTCAA CAATGGACGC
    ACAACTCTTG ACAACACAAT AAAAGGGTCT TCATCCTGGG CCTCAGAGAA ATCCAAGGAA
    GTCCAAAAGT CTAAACGGAG ACCAATGTCT AGAGGTCAAT CTGAGGATGC CTCAAATGCA
    AACACTGCCC AGGAGTCAGT AGAAATGAGA AAGATGGAGA ACAGTGCCAA CACAGTGACC
    TCTGGTGGCC TTGAGGGCCA AGGACGTGCA GCGTCCATGC CAAAACTCAA TGCTGAGATG
    CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA ACATTACTGG AACCCATTCC AGATTCCCCC
    ATGAGGCGCT CTGCATCCAC TTTTGCACCA CAGCGCCCTC ACGAGGTGAA TCTGAATGAC
    TACATTCTGG AGAAACCTGT TCAGGAACGC CATCACCACC ATCACCATCA TCACCGATGC
    CATCATCGCC GGGACAGAGA CAAGAAACAG AGATCTCTGG ACAGGTCGCC TACTGGCCAT
    CACAAAACTA CAGGCACAGC CACTGACTCT GTAGAGGAGA GGGCACATGA CCGGGGTCGT
    TCCCATGAGA GGAAGCACCA TTCATCTTCA GGAGAGAAGC AGCGGTATTA CTCGTGTGAT
    CGCTATGCCA GTCGGGAACA CTGTTCCAGC AAATCTGCCG TGGCAAGCTG TGCCACCTCA
    CCCAGCGAGA CCCAGGAGGC CAATCTTAAC AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC
    CCGGTGTTGT TGACTTCAGG GGCGAGCACT CCAAGCCGCG GGCGCAGACA GCTGCCCCAG
    ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC TACAGGACTG CCAACTCTTC CCCTGTGCAA
    CTTCTGGCCA CAACGACCAG CTTGCTTCCC CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG
    TCCGAGCACA ACGCTCTTTT ACTCAGCGGC TCTATCTCCA GCTCGCCGGC CCCCGTCAGT
    CGCATTAACT CAGAACCCTT CTTGGGGCCC CCGGACCACC CTGCTGGCTT GCAGCCCGAA
    GCCTTCGCTG TCTTCCAAGA GAATCCGAGC CCTCAGATGG GCCGTTCTCC TCAGACTGTA
    GGGATGCCCT TCGTGGCGAT CCCCCCACCT CCCCCACAGC AGTTTCGCAG GGTCCCCAAC
    GGGTACCACT TCTCCACGGG CCCCAGCGCC AGCAGAGGAC GGGCACAGCA GTACTACCCA
    GAGGCAGAGG TGGAGGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42258
    Clone ID Related Accession (Same CDS sequence) XM_009301494.2
    Accession Version XM_009301494.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CATCACATCA AAGCGTAAAC
    AGAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG
    CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG
    AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC
    GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG
    GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA
    CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC
    ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT
    GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC
    GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC
    ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG
    AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC
    CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC
    ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT
    GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG
    GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT
    GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC
    ACAACGGAGC GTCAGCTGAA CTTCAGTTTC CTGAGGCTCT TTAGAGCAGC CCGACTCATC
    AAGCTCCTCC GGCAGGGATA CACCATTCGC ATCCTGCTCT GGACATTTGT CCAGTCTTTT
    AAGGCACTGC CTTATGTTTG CCTTCTAATC GCCATGCTGT TTTTCATCTA CGCCATTATT
    GGGATGCAGG TGTTTGGAAA CATTGATTTG AATGATGACA CGGCAATCAA CCGTCACAAC
    AACTTCCGCA CTTTCCTCCA AGCTCTTATG CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT
    TGGCATGATA TCATGCTGTC GTGTCTGAGC GAGCGCACCT GTGATCCGAG CTCAGGCACC
    CTTGGAAAGG AGTGTGGGAG TGACTTTGCC TACTTCTACT TTGTCTCCTT CATTTTCCTC
    TGCTCATTTC TGATGTTGAA CCTGTTTGTG GCCGTCATTA TGGACAACTT TGAATATCTG
    ACACGCGACT CCTCCATCCT GGGCCCTCAC CACCTGGATG AGTTTATTCG AGTGTGGGCG
    GAGTATGACC CAGCCGCATG TGGCCGTATC AAGTACCTCG ATATGTATGA GATGCTGCTC
    CACATGTCCC CACCTTTAGG TTTGGGAAAG AAATGTCCTC CACGAATCGC CTACAAGCGT
    CTGGTGAAAA TGAACATGCC CATTGCGGAT GACAACAGTG TACACTTTAC CTCAACGCTG
    ATGGCTCTGA TCCGTACTGC CCTGGAGATC AAACTGGCTT CAGGCATGGT GGCTCAGCGT
    CTGTCAGATG CTGAACTGAA GAAGGAGCTC TCTACAGTTT GGCCAAACCT GTCTCAGAAG
    ACCATGGACC TGCTGGTCAC TCCACATAAA CCTAATGAGC TCACAGTGGG GAAGGTGTAT
    GCAGCTCTTA TGATCTTTGA CTATTACAAG CAGAACAGAG CAAAAAGACT TCAGATGCAG
    CAACAGCAGC TGAAAGAACA GGCAGGACCT GGATCTCAGA ATAAAGTCAG CGCTCTCTTA
    GAGCCCATTC TCCCACTAAC CCACATGCAA GATCAGCCAG TGAACGGTGT CGATTCAGCT
    TCAGAGTGTC AAGCTCAGAC CAGACCCAGC TCCACCACAC TCAACAATGG ACGCACAACT
    CTTGACAACA CAATAAAAGG GTCTTCATCC TGGGCCTCAG AGAAATCCAA GGAAGTCCAA
    AAGTCTAAAC GGAGACCAAT GTCTAGAGGT CAATCTGAGG ATGCCTCAAA TGCAAACACT
    GCCCAGGAGT CAGTAGAAAT GAGAAAGATG GAGAACAGTG CCAACACAGT GACCTCTGGT
    GGCCTTGAGG GCCAAGGACG TGCAGCGTCC ATGCCAAAAC TCAATGCTGA GATGCAGAGG
    AGTCATTCCC GTCAATCTCC TGGAACATTA CTGGAACCCA TTCCAGATTC CCCCATGAGG
    CGCTCTGCAT CCACTTTTGC ACCACAGCGC CCTCACGAGG TGAATCTGAA TGACTACATT
    CTGGAGAAAC CTGTTCAGGA ACGCCATCAC CACCATCACC ATCATCACCG ATGCCATCAT
    CGCCGGGACA GAGACAAGAA ACAGAGATCT CTGGACAGGT CGCCTACTGG CCATCACAAA
    ACTACAGGCA CAGCCACTGA CTCTGTAGAG GAGAGGGCAC ATGACCGGGG TCGTTCCCAT
    GAGAGGAAGC ACCATTCATC TTCAGGAGAG AAGCAGCGGT ATTACTCGTG TGATCGCTAT
    GCCAGTCGGG AACACTGTTC CAGCAAATCT GCCGTGGCAA GCTGTGCCAC CTCACCCAGC
    GAGACCCAGG AGGCCAATCT TAACAAGCAG GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG
    TTGTTGACTT CAGGGGCGAG CACTCCAAGC CGCGGGCGCA GACAGCTGCC CCAGACCCCC
    CTCACTCCTC GTCCCTCCGT GGCCTACAGG ACTGCCAACT CTTCCCCTGT GCAACTTCTG
    GCCACAACGA CCAGCTTGCT TCCCCCGAGT CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG
    CACAACGCTC TTTTACTCAG CGGCTCTATC TCCAGCTCGC CGGCCCCCGT CAGTCGCATT
    AACTCAGAAC CCTTCTTGGG GCCCCCGGAC CACCCTGCTG GCTTGCAGCC CGAAGCCTTC
    GCTGTCTTCC AAGAGAATCC GAGCCCTCAG ATGGGCCGTT CTCCTCAGAC TGTAGGGATG
    CCCTTCGTGG CGATCCCCCC ACCTCCCCCA CAGCAGTTTC GCAGGGTCCC CAACGGGTAC
    CACTTCTCCA CGGGCCCCAG CGCCAGCAGA GGACGGGCAC AGCAGTACTA CCCAGAGGCA
    GAGGTGGAGG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299769.1
    CDS636..7796
    Translation

    Target ORF information:

    RefSeq Version XM_009301494.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301494.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CATCACATCA AAGCGTAAAC
    AGAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG
    CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG
    AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC
    GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG
    GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA
    CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC
    ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT
    GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC
    GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC
    ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG
    AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC
    CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC
    ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT
    GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG
    GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT
    GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC
    ACAACGGAGC GTCAGCTGAA CTTCAGTTTC CTGAGGCTCT TTAGAGCAGC CCGACTCATC
    AAGCTCCTCC GGCAGGGATA CACCATTCGC ATCCTGCTCT GGACATTTGT CCAGTCTTTT
    AAGGCACTGC CTTATGTTTG CCTTCTAATC GCCATGCTGT TTTTCATCTA CGCCATTATT
    GGGATGCAGG TGTTTGGAAA CATTGATTTG AATGATGACA CGGCAATCAA CCGTCACAAC
    AACTTCCGCA CTTTCCTCCA AGCTCTTATG CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT
    TGGCATGATA TCATGCTGTC GTGTCTGAGC GAGCGCACCT GTGATCCGAG CTCAGGCACC
    CTTGGAAAGG AGTGTGGGAG TGACTTTGCC TACTTCTACT TTGTCTCCTT CATTTTCCTC
    TGCTCATTTC TGATGTTGAA CCTGTTTGTG GCCGTCATTA TGGACAACTT TGAATATCTG
    ACACGCGACT CCTCCATCCT GGGCCCTCAC CACCTGGATG AGTTTATTCG AGTGTGGGCG
    GAGTATGACC CAGCCGCATG TGGCCGTATC AAGTACCTCG ATATGTATGA GATGCTGCTC
    CACATGTCCC CACCTTTAGG TTTGGGAAAG AAATGTCCTC CACGAATCGC CTACAAGCGT
    CTGGTGAAAA TGAACATGCC CATTGCGGAT GACAACAGTG TACACTTTAC CTCAACGCTG
    ATGGCTCTGA TCCGTACTGC CCTGGAGATC AAACTGGCTT CAGGCATGGT GGCTCAGCGT
    CTGTCAGATG CTGAACTGAA GAAGGAGCTC TCTACAGTTT GGCCAAACCT GTCTCAGAAG
    ACCATGGACC TGCTGGTCAC TCCACATAAA CCTAATGAGC TCACAGTGGG GAAGGTGTAT
    GCAGCTCTTA TGATCTTTGA CTATTACAAG CAGAACAGAG CAAAAAGACT TCAGATGCAG
    CAACAGCAGC TGAAAGAACA GGCAGGACCT GGATCTCAGA ATAAAGTCAG CGCTCTCTTA
    GAGCCCATTC TCCCACTAAC CCACATGCAA GATCAGCCAG TGAACGGTGT CGATTCAGCT
    TCAGAGTGTC AAGCTCAGAC CAGACCCAGC TCCACCACAC TCAACAATGG ACGCACAACT
    CTTGACAACA CAATAAAAGG GTCTTCATCC TGGGCCTCAG AGAAATCCAA GGAAGTCCAA
    AAGTCTAAAC GGAGACCAAT GTCTAGAGGT CAATCTGAGG ATGCCTCAAA TGCAAACACT
    GCCCAGGAGT CAGTAGAAAT GAGAAAGATG GAGAACAGTG CCAACACAGT GACCTCTGGT
    GGCCTTGAGG GCCAAGGACG TGCAGCGTCC ATGCCAAAAC TCAATGCTGA GATGCAGAGG
    AGTCATTCCC GTCAATCTCC TGGAACATTA CTGGAACCCA TTCCAGATTC CCCCATGAGG
    CGCTCTGCAT CCACTTTTGC ACCACAGCGC CCTCACGAGG TGAATCTGAA TGACTACATT
    CTGGAGAAAC CTGTTCAGGA ACGCCATCAC CACCATCACC ATCATCACCG ATGCCATCAT
    CGCCGGGACA GAGACAAGAA ACAGAGATCT CTGGACAGGT CGCCTACTGG CCATCACAAA
    ACTACAGGCA CAGCCACTGA CTCTGTAGAG GAGAGGGCAC ATGACCGGGG TCGTTCCCAT
    GAGAGGAAGC ACCATTCATC TTCAGGAGAG AAGCAGCGGT ATTACTCGTG TGATCGCTAT
    GCCAGTCGGG AACACTGTTC CAGCAAATCT GCCGTGGCAA GCTGTGCCAC CTCACCCAGC
    GAGACCCAGG AGGCCAATCT TAACAAGCAG GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG
    TTGTTGACTT CAGGGGCGAG CACTCCAAGC CGCGGGCGCA GACAGCTGCC CCAGACCCCC
    CTCACTCCTC GTCCCTCCGT GGCCTACAGG ACTGCCAACT CTTCCCCTGT GCAACTTCTG
    GCCACAACGA CCAGCTTGCT TCCCCCGAGT CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG
    CACAACGCTC TTTTACTCAG CGGCTCTATC TCCAGCTCGC CGGCCCCCGT CAGTCGCATT
    AACTCAGAAC CCTTCTTGGG GCCCCCGGAC CACCCTGCTG GCTTGCAGCC CGAAGCCTTC
    GCTGTCTTCC AAGAGAATCC GAGCCCTCAG ATGGGCCGTT CTCCTCAGAC TGTAGGGATG
    CCCTTCGTGG CGATCCCCCC ACCTCCCCCA CAGCAGTTTC GCAGGGTCCC CAACGGGTAC
    CACTTCTCCA CGGGCCCCAG CGCCAGCAGA GGACGGGCAC AGCAGTACTA CCCAGAGGCA
    GAGGTGGAGG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42259
    Clone ID Related Accession (Same CDS sequence) XM_009301495.2
    Accession Version XM_009301495.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGAGGAACC
    AAGGGCAAAG ACATCAGCAC TATCAAGTCA CTGAGGGTGT TGCGAGTCTT GCGACCTCTG
    AAGACCATCA AACGTCTACC AAAACTGAAG GCCGTGTTTG ATTGTGTGGT GAACTCCCTG
    AAGAATGTCC TGAATATCCT CATTGTCTAC ATTCTGTTCA TGTTCATTTT CGCTGTCATT
    GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT TACTGCACAG ACGAGTCCAA GGGCCTGGAG
    AAAGACTGCA GGGGGCTGTT TTTGGATTAT GATAAGGATG ATGTGACGGC ACAACCGAGG
    GAATGGAAGA AATACGAGTT TCACTATGAC AATGTGCTTT GGGCTTTTCT CACTCTCTTC
    ACTGTTTCTA CGGGAGAGGG CTGGCCTACG GTTTTGAAGC ACTCCGTGGA TGCCACTTTT
    GAGGATCAGG GTCCCAGTCC AGGCTACCGC ATTGAGATGT CTATTTTTTA CGTGGTCTAC
    TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC ATTTTTGTAG CCTTGATAAT CATCACATTC
    CAGGAACAGG GTGATAAGGT CTTGTCAGAG TGCAGTCTGG AGAAGAATGA GAGAGCCTGC
    ATCGACTTTG CTATCAATGC CAAACCTCTG ACACGCTACA TGCCGCAGAA CCAGCAGTCC
    TTTCAGTACC GCCTGTGGAA GTTTGTGGTC TCTCCGCCGT TTGAGTACTC CATCATGATC
    ATGATTGCTC TCAACACTGT GGTGCTCATG ATGAAGTTCC ATGGGGCGCC TGACTTTTAT
    GAGGCAATGC TTAAGTATCT CAACATTGTC TTCACTGTTC TCTTTTCACT GGAGTGTATT
    CTCAAGATTA TTGCTTTCGG CCCCTTGAAT TACCTGAAAG ATGCCTGGAA TGTGTTTGAC
    TTTGTGACTG TGCTGGGCAG CATCACTGAC ATTTTAGTCA CTGAGATTAA CACAACGGAG
    CGTCAGCTGA ACTTCAGTTT CCTGAGGCTC TTTAGAGCAG CCCGACTCAT CAAGCTCCTC
    CGGCAGGGAT ACACCATTCG CATCCTGCTC TGGACATTTG TCCAGTCTTT TAAGGCACTG
    CCTTATGTTT GCCTTCTAAT CGCCATGCTG TTTTTCATCT ACGCCATTAT TGGGATGCAG
    GTGTTTGGAA ACATTGATTT GAATGATGAC ACGGCAATCA ACCGTCACAA CAACTTCCGC
    ACTTTCCTCC AAGCTCTTAT GCTTTTGTTC AGGAGTGCCA CTGGCGAGGC TTGGCATGAT
    ATCATGCTGT CGTGTCTGAG CGAGCGCACC TGTGATCCGA GCTCAGGCAC CCTTGGAAAG
    GAGTGTGGGA GTGACTTTGC CTACTTCTAC TTTGTCTCCT TCATTTTCCT CTGCTCATTT
    CTGATGTTGA ACCTGTTTGT GGCCGTCATT ATGGACAACT TTGAATATCT GACACGCGAC
    TCCTCCATCC TGGGCCCTCA CCACCTGGAT GAGTTTATTC GAGTGTGGGC GGAGTATGAC
    CCAGCCGCAT GTGGCCGTAT CAAGTACCTC GATATGTATG AGATGCTGCT CCACATGTCC
    CCACCTTTAG GTTTGGGAAA GAAATGTCCT CCACGAATCG CCTACAAGCG TCTGGTGAAA
    ATGAACATGC CCATTGCGGA TGACAACAGT GTACACTTTA CCTCAACGCT GATGGCTCTG
    ATCCGTACTG CCCTGGAGAT CAAACTGGCT TCAGGCATGG TGGCTCAGCG TCTGTCAGAT
    GCTGAACTGA AGAAGGAGCT CTCTACAGTT TGGCCAAACC TGTCTCAGAA GACCATGGAC
    CTGCTGGTCA CTCCACATAA ACCTAATGAG CTCACAGTGG GGAAGGTGTA TGCAGCTCTT
    ATGATCTTTG ACTATTACAA GCAGAACAGA GCAAAAAGAC TTCAGATGCA GCAACAGCAG
    CTGAAAGAAC AGGCAGGACC TGGATCTCAG AATAAAGTCA GCGCTCTCTT AGAGCCCATT
    CTCCCACTAA CCCACATGCA AGATCAGCCA GTGAACGGTG TCGATTCAGC TTCAGAGTGT
    CAAGCTCAGA CCAGACCCAG CTCCACCACA CTCAACAATG GACGCACAAC TCTTGACAAC
    ACAATAAAAG GGTCTTCATC CTGGGCCTCA GAGAAATCCA AGGAAGTCCA AAAGTCTAAA
    CGGAGACCAA TGTCTAGAGG TCAATCTGAG GATGCCTCAA ATGCAAACAC TGCCCAGGAG
    TCAGTAGAAA TGAGAAAGAT GGAGAACAGT GCCAACACAG TGACCTCTGG TGGCCTTGAG
    GGCCAAGGAC GTGCAGCGTC CATGCCAAAA CTCAATGCTG AGATGCAGAG GAGTCATTCC
    CGTCAATCTC CTGGAACATT ACTGGAACCC ATTCCAGATT CCCCCATGAG GCGCTCTGCA
    TCCACTTTTG CACCACAGCG CCCTCACGAG GTGAATCTGA ATGACTACAT TCTGGAGAAA
    CCTGTTCAGG AACGCCATCA CCACCATCAC CATCATCACC GATGCCATCA TCGCCGGGAC
    AGAGACAAGA AACAGAGATC TCTGGACAGG TCGCCTACTG GCCATCACAA AACTACAGGC
    ACAGCCACTG ACTCTGTAGA GGAGAGGGCA CATGACCGGG GTCGTTCCCA TGAGAGGAAG
    CACCATTCAT CTTCAGGAGA GAAGCAGCGG TATTACTCGT GTGATCGCTA TGCCAGTCGG
    GAACACTGTT CCAGCAAATC TGCCGTGGCA AGCTGTGCCA CCTCACCCAG CGAGACCCAG
    GAGGCCAATC TTAACAAGCA GGGCAGTGGG TGGGCTAAGG GGAGCCCGGT GTTGTTGACT
    TCAGGGGCGA GCACTCCAAG CCGCGGGCGC AGACAGCTGC CCCAGACCCC CCTCACTCCT
    CGTCCCTCCG TGGCCTACAG GACTGCCAAC TCTTCCCCTG TGCAACTTCT GGCCACAACG
    ACCAGCTTGC TTCCCCCGAG TCCCGGCAGG CTGAGCCGTG GTCTGTCCGA GCACAACGCT
    CTTTTACTCA GCGGCTCTAT CTCCAGCTCG CCGGCCCCCG TCAGTCGCAT TAACTCAGAA
    CCCTTCTTGG GGCCCCCGGA CCACCCTGCT GGCTTGCAGC CCGAAGCCTT CGCTGTCTTC
    CAAGAGAATC CGAGCCCTCA GATGGGCCGT TCTCCTCAGA CTGTAGGGAT GCCCTTCGTG
    GCGATCCCCC CACCTCCCCC ACAGCAGTTT CGCAGGGTCC CCAACGGGTA CCACTTCTCC
    ACGGGCCCCA GCGCCAGCAG AGGACGGGCA CAGCAGTACT ACCCAGAGGC AGAGGTGGAG
    GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299770.1
    CDS636..7787
    Translation

    Target ORF information:

    RefSeq Version XM_009301495.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301495.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGAGGAACC
    AAGGGCAAAG ACATCAGCAC TATCAAGTCA CTGAGGGTGT TGCGAGTCTT GCGACCTCTG
    AAGACCATCA AACGTCTACC AAAACTGAAG GCCGTGTTTG ATTGTGTGGT GAACTCCCTG
    AAGAATGTCC TGAATATCCT CATTGTCTAC ATTCTGTTCA TGTTCATTTT CGCTGTCATT
    GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT TACTGCACAG ACGAGTCCAA GGGCCTGGAG
    AAAGACTGCA GGGGGCTGTT TTTGGATTAT GATAAGGATG ATGTGACGGC ACAACCGAGG
    GAATGGAAGA AATACGAGTT TCACTATGAC AATGTGCTTT GGGCTTTTCT CACTCTCTTC
    ACTGTTTCTA CGGGAGAGGG CTGGCCTACG GTTTTGAAGC ACTCCGTGGA TGCCACTTTT
    GAGGATCAGG GTCCCAGTCC AGGCTACCGC ATTGAGATGT CTATTTTTTA CGTGGTCTAC
    TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC ATTTTTGTAG CCTTGATAAT CATCACATTC
    CAGGAACAGG GTGATAAGGT CTTGTCAGAG TGCAGTCTGG AGAAGAATGA GAGAGCCTGC
    ATCGACTTTG CTATCAATGC CAAACCTCTG ACACGCTACA TGCCGCAGAA CCAGCAGTCC
    TTTCAGTACC GCCTGTGGAA GTTTGTGGTC TCTCCGCCGT TTGAGTACTC CATCATGATC
    ATGATTGCTC TCAACACTGT GGTGCTCATG ATGAAGTTCC ATGGGGCGCC TGACTTTTAT
    GAGGCAATGC TTAAGTATCT CAACATTGTC TTCACTGTTC TCTTTTCACT GGAGTGTATT
    CTCAAGATTA TTGCTTTCGG CCCCTTGAAT TACCTGAAAG ATGCCTGGAA TGTGTTTGAC
    TTTGTGACTG TGCTGGGCAG CATCACTGAC ATTTTAGTCA CTGAGATTAA CACAACGGAG
    CGTCAGCTGA ACTTCAGTTT CCTGAGGCTC TTTAGAGCAG CCCGACTCAT CAAGCTCCTC
    CGGCAGGGAT ACACCATTCG CATCCTGCTC TGGACATTTG TCCAGTCTTT TAAGGCACTG
    CCTTATGTTT GCCTTCTAAT CGCCATGCTG TTTTTCATCT ACGCCATTAT TGGGATGCAG
    GTGTTTGGAA ACATTGATTT GAATGATGAC ACGGCAATCA ACCGTCACAA CAACTTCCGC
    ACTTTCCTCC AAGCTCTTAT GCTTTTGTTC AGGAGTGCCA CTGGCGAGGC TTGGCATGAT
    ATCATGCTGT CGTGTCTGAG CGAGCGCACC TGTGATCCGA GCTCAGGCAC CCTTGGAAAG
    GAGTGTGGGA GTGACTTTGC CTACTTCTAC TTTGTCTCCT TCATTTTCCT CTGCTCATTT
    CTGATGTTGA ACCTGTTTGT GGCCGTCATT ATGGACAACT TTGAATATCT GACACGCGAC
    TCCTCCATCC TGGGCCCTCA CCACCTGGAT GAGTTTATTC GAGTGTGGGC GGAGTATGAC
    CCAGCCGCAT GTGGCCGTAT CAAGTACCTC GATATGTATG AGATGCTGCT CCACATGTCC
    CCACCTTTAG GTTTGGGAAA GAAATGTCCT CCACGAATCG CCTACAAGCG TCTGGTGAAA
    ATGAACATGC CCATTGCGGA TGACAACAGT GTACACTTTA CCTCAACGCT GATGGCTCTG
    ATCCGTACTG CCCTGGAGAT CAAACTGGCT TCAGGCATGG TGGCTCAGCG TCTGTCAGAT
    GCTGAACTGA AGAAGGAGCT CTCTACAGTT TGGCCAAACC TGTCTCAGAA GACCATGGAC
    CTGCTGGTCA CTCCACATAA ACCTAATGAG CTCACAGTGG GGAAGGTGTA TGCAGCTCTT
    ATGATCTTTG ACTATTACAA GCAGAACAGA GCAAAAAGAC TTCAGATGCA GCAACAGCAG
    CTGAAAGAAC AGGCAGGACC TGGATCTCAG AATAAAGTCA GCGCTCTCTT AGAGCCCATT
    CTCCCACTAA CCCACATGCA AGATCAGCCA GTGAACGGTG TCGATTCAGC TTCAGAGTGT
    CAAGCTCAGA CCAGACCCAG CTCCACCACA CTCAACAATG GACGCACAAC TCTTGACAAC
    ACAATAAAAG GGTCTTCATC CTGGGCCTCA GAGAAATCCA AGGAAGTCCA AAAGTCTAAA
    CGGAGACCAA TGTCTAGAGG TCAATCTGAG GATGCCTCAA ATGCAAACAC TGCCCAGGAG
    TCAGTAGAAA TGAGAAAGAT GGAGAACAGT GCCAACACAG TGACCTCTGG TGGCCTTGAG
    GGCCAAGGAC GTGCAGCGTC CATGCCAAAA CTCAATGCTG AGATGCAGAG GAGTCATTCC
    CGTCAATCTC CTGGAACATT ACTGGAACCC ATTCCAGATT CCCCCATGAG GCGCTCTGCA
    TCCACTTTTG CACCACAGCG CCCTCACGAG GTGAATCTGA ATGACTACAT TCTGGAGAAA
    CCTGTTCAGG AACGCCATCA CCACCATCAC CATCATCACC GATGCCATCA TCGCCGGGAC
    AGAGACAAGA AACAGAGATC TCTGGACAGG TCGCCTACTG GCCATCACAA AACTACAGGC
    ACAGCCACTG ACTCTGTAGA GGAGAGGGCA CATGACCGGG GTCGTTCCCA TGAGAGGAAG
    CACCATTCAT CTTCAGGAGA GAAGCAGCGG TATTACTCGT GTGATCGCTA TGCCAGTCGG
    GAACACTGTT CCAGCAAATC TGCCGTGGCA AGCTGTGCCA CCTCACCCAG CGAGACCCAG
    GAGGCCAATC TTAACAAGCA GGGCAGTGGG TGGGCTAAGG GGAGCCCGGT GTTGTTGACT
    TCAGGGGCGA GCACTCCAAG CCGCGGGCGC AGACAGCTGC CCCAGACCCC CCTCACTCCT
    CGTCCCTCCG TGGCCTACAG GACTGCCAAC TCTTCCCCTG TGCAACTTCT GGCCACAACG
    ACCAGCTTGC TTCCCCCGAG TCCCGGCAGG CTGAGCCGTG GTCTGTCCGA GCACAACGCT
    CTTTTACTCA GCGGCTCTAT CTCCAGCTCG CCGGCCCCCG TCAGTCGCAT TAACTCAGAA
    CCCTTCTTGG GGCCCCCGGA CCACCCTGCT GGCTTGCAGC CCGAAGCCTT CGCTGTCTTC
    CAAGAGAATC CGAGCCCTCA GATGGGCCGT TCTCCTCAGA CTGTAGGGAT GCCCTTCGTG
    GCGATCCCCC CACCTCCCCC ACAGCAGTTT CGCAGGGTCC CCAACGGGTA CCACTTCTCC
    ACGGGCCCCA GCGCCAGCAG AGGACGGGCA CAGCAGTACT ACCCAGAGGC AGAGGTGGAG
    GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42260
    Clone ID Related Accession (Same CDS sequence) XM_009301496.2
    Accession Version XM_009301496.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CAGGAACCAA GGGCAAAGAC
    ATCAGCACTA TCAAGTCACT GAGGGTGTTG CGAGTCTTGC GACCTCTGAA GACCATCAAA
    CGTCTACCAA AACTGAAGGC CGTGTTTGAT TGTGTGGTGA ACTCCCTGAA GAATGTCCTG
    AATATCCTCA TTGTCTACAT TCTGTTCATG TTCATTTTCG CTGTCATTGC TGTGCAGCTC
    TTCAAGGGCA AGTTTTTTTA CTGCACAGAC GAGTCCAAGG GCCTGGAGAA AGACTGCAGG
    GGGCTGTTTT TGGATTATGA TAAGGATGAT GTGACGGCAC AACCGAGGGA ATGGAAGAAA
    TACGAGTTTC ACTATGACAA TGTGCTTTGG GCTTTTCTCA CTCTCTTCAC TGTTTCTACG
    GGAGAGGGCT GGCCTACGGT TTTGAAGCAC TCCGTGGATG CCACTTTTGA GGATCAGGGT
    CCCAGTCCAG GCTACCGCAT TGAGATGTCT ATTTTTTACG TGGTCTACTT TGTGGTTTTC
    CCTTTCTTCT TCGTCAACAT TTTTGTAGCC TTGATAATCA TCACATTCCA GGAACAGGGT
    GATAAGGTCT TGTCAGAGTG CAGTCTGGAG AAGAATGAGA GAGCCTGCAT CGACTTTGCT
    ATCAATGCCA AACCTCTGAC ACGCTACATG CCGCAGAACC AGCAGTCCTT TCAGTACCGC
    CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT GAGTACTCCA TCATGATCAT GATTGCTCTC
    AACACTGTGG TGCTCATGAT GAAGTTCCAT GGGGCGCCTG ACTTTTATGA GGCAATGCTT
    AAGTATCTCA ACATTGTCTT CACTGTTCTC TTTTCACTGG AGTGTATTCT CAAGATTATT
    GCTTTCGGCC CCTTGAATTA CCTGAAAGAT GCCTGGAATG TGTTTGACTT TGTGACTGTG
    CTGGGCAGCA TCACTGACAT TTTAGTCACT GAGATTAACA CAACGGAGCG TCAGCTGAAC
    TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC CGACTCATCA AGCTCCTCCG GCAGGGATAC
    ACCATTCGCA TCCTGCTCTG GACATTTGTC CAGTCTTTTA AGGCACTGCC TTATGTTTGC
    CTTCTAATCG CCATGCTGTT TTTCATCTAC GCCATTATTG GGATGCAGGT GTTTGGAAAC
    ATTGATTTGA ATGATGACAC GGCAATCAAC CGTCACAACA ACTTCCGCAC TTTCCTCCAA
    GCTCTTATGC TTTTGTTCAG GAGTGCCACT GGCGAGGCTT GGCATGATAT CATGCTGTCG
    TGTCTGAGCG AGCGCACCTG TGATCCGAGC TCAGGCACCC TTGGAAAGGA GTGTGGGAGT
    GACTTTGCCT ACTTCTACTT TGTCTCCTTC ATTTTCCTCT GCTCATTTCT GATGTTGAAC
    CTGTTTGTGG CCGTCATTAT GGACAACTTT GAATATCTGA CACGCGACTC CTCCATCCTG
    GGCCCTCACC ACCTGGATGA GTTTATTCGA GTGTGGGCGG AGTATGACCC AGCCGCATGT
    GGCCGTATCA AGTACCTCGA TATGTATGAG ATGCTGCTCC ACATGTCCCC ACCTTTAGGT
    TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGTC TGGTGAAAAT GAACATGCCC
    ATTGCGGATG ACAACAGTGT ACACTTTACC TCAACGCTGA TGGCTCTGAT CCGTACTGCC
    CTGGAGATCA AACTGGCTTC AGGCATGGTG GCTCAGCGTC TGTCAGATGC TGAACTGAAG
    AAGGAGCTCT CTACAGTTTG GCCAAACCTG TCTCAGAAGA CCATGGACCT GCTGGTCACT
    CCACATAAAC CTAATGAGCT CACAGTGGGG AAGGTGTATG CAGCTCTTAT GATCTTTGAC
    TATTACAAGC AGAACAGAGC AAAAAGACTT CAGATGCAGC AACAGCAGCT GAAAGAACAG
    GCAGGACCTG GATCTCAGAA TAAAGTCAGC GCTCTCTTAG AGCCCATTCT CCCACTAACC
    CACATGCAAG ATCAGCCAGT GAACGGTGTC GATTCAGCTT CAGAGTGTCA AGCTCAGACC
    AGACCCAGCT CCACCACACT CAACAATGGA CGCACAACTC TTGACAACAC AATAAAAGGG
    TCTTCATCCT GGGCCTCAGA GAAATCCAAG GAAGTCCAAA AGTCTAAACG GAGACCAATG
    TCTAGAGGTC AATCTGAGGA TGCCTCAAAT GCAAACACTG CCCAGGAGTC AGTAGAAATG
    AGAAAGATGG AGAACAGTGC CAACACAGTG ACCTCTGGTG GCCTTGAGGG CCAAGGACGT
    GCAGCGTCCA TGCCAAAACT CAATGCTGAG ATGCAGAGGA GTCATTCCCG TCAATCTCCT
    GGAACATTAC TGGAACCCAT TCCAGATTCC CCCATGAGGC GCTCTGCATC CACTTTTGCA
    CCACAGCGCC CTCACGAGGT GAATCTGAAT GACTACATTC TGGAGAAACC TGTTCAGGAA
    CGCCATCACC ACCATCACCA TCATCACCGA TGCCATCATC GCCGGGACAG AGACAAGAAA
    CAGAGATCTC TGGACAGGTC GCCTACTGGC CATCACAAAA CTACAGGCAC AGCCACTGAC
    TCTGTAGAGG AGAGGGCACA TGACCGGGGT CGTTCCCATG AGAGGAAGCA CCATTCATCT
    TCAGGAGAGA AGCAGCGGTA TTACTCGTGT GATCGCTATG CCAGTCGGGA ACACTGTTCC
    AGCAAATCTG CCGTGGCAAG CTGTGCCACC TCACCCAGCG AGACCCAGGA GGCCAATCTT
    AACAAGCAGG GCAGTGGGTG GGCTAAGGGG AGCCCGGTGT TGTTGACTTC AGGGGCGAGC
    ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC CAGACCCCCC TCACTCCTCG TCCCTCCGTG
    GCCTACAGGA CTGCCAACTC TTCCCCTGTG CAACTTCTGG CCACAACGAC CAGCTTGCTT
    CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT CTGTCCGAGC ACAACGCTCT TTTACTCAGC
    GGCTCTATCT CCAGCTCGCC GGCCCCCGTC AGTCGCATTA ACTCAGAACC CTTCTTGGGG
    CCCCCGGACC ACCCTGCTGG CTTGCAGCCC GAAGCCTTCG CTGTCTTCCA AGAGAATCCG
    AGCCCTCAGA TGGGCCGTTC TCCTCAGACT GTAGGGATGC CCTTCGTGGC GATCCCCCCA
    CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC AACGGGTACC ACTTCTCCAC GGGCCCCAGC
    GCCAGCAGAG GACGGGCACA GCAGTACTAC CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299771.1
    CDS636..7775
    Translation

    Target ORF information:

    RefSeq Version XM_009301496.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CAGGAACCAA GGGCAAAGAC
    ATCAGCACTA TCAAGTCACT GAGGGTGTTG CGAGTCTTGC GACCTCTGAA GACCATCAAA
    CGTCTACCAA AACTGAAGGC CGTGTTTGAT TGTGTGGTGA ACTCCCTGAA GAATGTCCTG
    AATATCCTCA TTGTCTACAT TCTGTTCATG TTCATTTTCG CTGTCATTGC TGTGCAGCTC
    TTCAAGGGCA AGTTTTTTTA CTGCACAGAC GAGTCCAAGG GCCTGGAGAA AGACTGCAGG
    GGGCTGTTTT TGGATTATGA TAAGGATGAT GTGACGGCAC AACCGAGGGA ATGGAAGAAA
    TACGAGTTTC ACTATGACAA TGTGCTTTGG GCTTTTCTCA CTCTCTTCAC TGTTTCTACG
    GGAGAGGGCT GGCCTACGGT TTTGAAGCAC TCCGTGGATG CCACTTTTGA GGATCAGGGT
    CCCAGTCCAG GCTACCGCAT TGAGATGTCT ATTTTTTACG TGGTCTACTT TGTGGTTTTC
    CCTTTCTTCT TCGTCAACAT TTTTGTAGCC TTGATAATCA TCACATTCCA GGAACAGGGT
    GATAAGGTCT TGTCAGAGTG CAGTCTGGAG AAGAATGAGA GAGCCTGCAT CGACTTTGCT
    ATCAATGCCA AACCTCTGAC ACGCTACATG CCGCAGAACC AGCAGTCCTT TCAGTACCGC
    CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT GAGTACTCCA TCATGATCAT GATTGCTCTC
    AACACTGTGG TGCTCATGAT GAAGTTCCAT GGGGCGCCTG ACTTTTATGA GGCAATGCTT
    AAGTATCTCA ACATTGTCTT CACTGTTCTC TTTTCACTGG AGTGTATTCT CAAGATTATT
    GCTTTCGGCC CCTTGAATTA CCTGAAAGAT GCCTGGAATG TGTTTGACTT TGTGACTGTG
    CTGGGCAGCA TCACTGACAT TTTAGTCACT GAGATTAACA CAACGGAGCG TCAGCTGAAC
    TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC CGACTCATCA AGCTCCTCCG GCAGGGATAC
    ACCATTCGCA TCCTGCTCTG GACATTTGTC CAGTCTTTTA AGGCACTGCC TTATGTTTGC
    CTTCTAATCG CCATGCTGTT TTTCATCTAC GCCATTATTG GGATGCAGGT GTTTGGAAAC
    ATTGATTTGA ATGATGACAC GGCAATCAAC CGTCACAACA ACTTCCGCAC TTTCCTCCAA
    GCTCTTATGC TTTTGTTCAG GAGTGCCACT GGCGAGGCTT GGCATGATAT CATGCTGTCG
    TGTCTGAGCG AGCGCACCTG TGATCCGAGC TCAGGCACCC TTGGAAAGGA GTGTGGGAGT
    GACTTTGCCT ACTTCTACTT TGTCTCCTTC ATTTTCCTCT GCTCATTTCT GATGTTGAAC
    CTGTTTGTGG CCGTCATTAT GGACAACTTT GAATATCTGA CACGCGACTC CTCCATCCTG
    GGCCCTCACC ACCTGGATGA GTTTATTCGA GTGTGGGCGG AGTATGACCC AGCCGCATGT
    GGCCGTATCA AGTACCTCGA TATGTATGAG ATGCTGCTCC ACATGTCCCC ACCTTTAGGT
    TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGTC TGGTGAAAAT GAACATGCCC
    ATTGCGGATG ACAACAGTGT ACACTTTACC TCAACGCTGA TGGCTCTGAT CCGTACTGCC
    CTGGAGATCA AACTGGCTTC AGGCATGGTG GCTCAGCGTC TGTCAGATGC TGAACTGAAG
    AAGGAGCTCT CTACAGTTTG GCCAAACCTG TCTCAGAAGA CCATGGACCT GCTGGTCACT
    CCACATAAAC CTAATGAGCT CACAGTGGGG AAGGTGTATG CAGCTCTTAT GATCTTTGAC
    TATTACAAGC AGAACAGAGC AAAAAGACTT CAGATGCAGC AACAGCAGCT GAAAGAACAG
    GCAGGACCTG GATCTCAGAA TAAAGTCAGC GCTCTCTTAG AGCCCATTCT CCCACTAACC
    CACATGCAAG ATCAGCCAGT GAACGGTGTC GATTCAGCTT CAGAGTGTCA AGCTCAGACC
    AGACCCAGCT CCACCACACT CAACAATGGA CGCACAACTC TTGACAACAC AATAAAAGGG
    TCTTCATCCT GGGCCTCAGA GAAATCCAAG GAAGTCCAAA AGTCTAAACG GAGACCAATG
    TCTAGAGGTC AATCTGAGGA TGCCTCAAAT GCAAACACTG CCCAGGAGTC AGTAGAAATG
    AGAAAGATGG AGAACAGTGC CAACACAGTG ACCTCTGGTG GCCTTGAGGG CCAAGGACGT
    GCAGCGTCCA TGCCAAAACT CAATGCTGAG ATGCAGAGGA GTCATTCCCG TCAATCTCCT
    GGAACATTAC TGGAACCCAT TCCAGATTCC CCCATGAGGC GCTCTGCATC CACTTTTGCA
    CCACAGCGCC CTCACGAGGT GAATCTGAAT GACTACATTC TGGAGAAACC TGTTCAGGAA
    CGCCATCACC ACCATCACCA TCATCACCGA TGCCATCATC GCCGGGACAG AGACAAGAAA
    CAGAGATCTC TGGACAGGTC GCCTACTGGC CATCACAAAA CTACAGGCAC AGCCACTGAC
    TCTGTAGAGG AGAGGGCACA TGACCGGGGT CGTTCCCATG AGAGGAAGCA CCATTCATCT
    TCAGGAGAGA AGCAGCGGTA TTACTCGTGT GATCGCTATG CCAGTCGGGA ACACTGTTCC
    AGCAAATCTG CCGTGGCAAG CTGTGCCACC TCACCCAGCG AGACCCAGGA GGCCAATCTT
    AACAAGCAGG GCAGTGGGTG GGCTAAGGGG AGCCCGGTGT TGTTGACTTC AGGGGCGAGC
    ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC CAGACCCCCC TCACTCCTCG TCCCTCCGTG
    GCCTACAGGA CTGCCAACTC TTCCCCTGTG CAACTTCTGG CCACAACGAC CAGCTTGCTT
    CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT CTGTCCGAGC ACAACGCTCT TTTACTCAGC
    GGCTCTATCT CCAGCTCGCC GGCCCCCGTC AGTCGCATTA ACTCAGAACC CTTCTTGGGG
    CCCCCGGACC ACCCTGCTGG CTTGCAGCCC GAAGCCTTCG CTGTCTTCCA AGAGAATCCG
    AGCCCTCAGA TGGGCCGTTC TCCTCAGACT GTAGGGATGC CCTTCGTGGC GATCCCCCCA
    CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC AACGGGTACC ACTTCTCCAC GGGCCCCAGC
    GCCAGCAGAG GACGGGCACA GCAGTACTAC CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42261
    Clone ID Related Accession (Same CDS sequence) XM_009301497.2 , XM_009301497.3
    Accession Version XM_009301497.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC
    AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC
    ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT
    CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT
    GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT
    CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT
    CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG
    TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC
    CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC
    CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG
    GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT
    GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG
    GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG
    GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA
    CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT
    TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA
    GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC
    ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA
    CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT
    TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT
    AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA
    AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA
    GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA
    ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA
    CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC
    CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG
    AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT
    GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA
    GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC
    AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC
    AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT
    CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC
    TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC
    CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC
    TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC
    CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC
    CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT
    CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC
    AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299772.1
    CDS636..7772
    Translation

    Target ORF information:

    RefSeq Version XM_009301497.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301497.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC
    AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC
    ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT
    CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT
    GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT
    CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT
    CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG
    TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC
    CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC
    CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG
    GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT
    GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG
    GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG
    GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA
    CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT
    TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA
    GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC
    ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA
    CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT
    TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT
    AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA
    AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA
    GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA
    ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA
    CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC
    CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG
    AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT
    GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA
    GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC
    AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC
    AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT
    CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC
    TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC
    CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC
    TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC
    CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC
    CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT
    CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC
    AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42261
    Clone ID Related Accession (Same CDS sequence) XM_009301497.2 , XM_009301497.3
    Accession Version XM_009301497.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC
    AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC
    ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT
    CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT
    GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT
    CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT
    CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG
    TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC
    CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC
    CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG
    GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT
    GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG
    GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG
    GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA
    CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT
    TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA
    GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC
    ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA
    CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT
    TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT
    AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA
    AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA
    GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA
    ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA
    CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC
    CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG
    AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT
    GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA
    GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC
    AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC
    AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT
    CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC
    TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC
    CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC
    TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC
    CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC
    CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT
    CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC
    AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299772.1
    CDS637..7773
    Translation

    Target ORF information:

    RefSeq Version XM_009301497.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301497.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC
    AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT
    CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT
    ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC
    AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG
    CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC
    GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA
    GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC
    AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT
    TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT
    AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC
    AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG
    TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC
    ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG
    TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT
    TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG
    GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC
    AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC
    ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT
    CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT
    GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT
    CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT
    CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG
    TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC
    CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC
    CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG
    GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT
    GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG
    GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG
    GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA
    CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT
    TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA
    GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC
    ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA
    CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT
    TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT
    AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA
    AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA
    GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA
    ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA
    CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC
    CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG
    AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT
    GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA
    GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC
    AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC
    AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT
    CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC
    TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC
    CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC
    TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC
    CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC
    CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT
    CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC
    AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42262
    Clone ID Related Accession (Same CDS sequence) XM_009301498.2
    Accession Version XM_009301498.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CAGGAACCAA GGGCAAAGAC
    ATCAGCACTA TCAAGTCACT GAGGGTGTTG CGAGTCTTGC GACCTCTGAA GACCATCAAA
    CGTCTACCAA AACTGAAGGC CGTGTTTGAT TGTGTGGTGA ACTCCCTGAA GAATGTCCTG
    AATATCCTCA TTGTCTACAT TCTGTTCATG TTCATTTTCG CTGTCATTGC TGTGCAGCTC
    TTCAAGGGCA AGTTTTTTTA CTGCACAGAC GAGTCCAAGG GCCTGGAGAA AGACTGCAGG
    GGGCTGTTTT TGGATTATGA TAAGGATGAT GTGACGGCAC AACCGAGGGA ATGGAAGAAA
    TACGAGTTTC ACTATGACAA TGTGCTTTGG GCTTTTCTCA CTCTCTTCAC TGTTTCTACG
    GGAGAGGGCT GGCCTACGGT TTTGAAGCAC TCCGTGGATG CCACTTTTGA GGATCAGGGT
    CCCAGTCCAG GCTACCGCAT TGAGATGTCT ATTTTTTACG TGGTCTACTT TGTGGTTTTC
    CCTTTCTTCT TCGTCAACAT TTTTGTAGCC TTGATAATCA TCACATTCCA GGAACAGGGT
    GATAAGGTCT TGTCAGAGTG CAGTCTGGAG AAGAATGAGA GAGCCTGCAT CGACTTTGCT
    ATCAATGCCA AACCTCTGAC ACGCTACATG CCGCAGAACC AGCAGTCCTT TCAGTACCGC
    CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT GAGTACTCCA TCATGATCAT GATTGCTCTC
    AACACTGTGG TGCTCATGAT GAAGTTCCAT GGGGCGCCTG ACTTTTATGA GGCAATGCTT
    AAGTATCTCA ACATTGTCTT CACTGTTCTC TTTTCACTGG AGTGTATTCT CAAGATTATT
    GCTTTCGGCC CCTTGAATTA CCTGAAAGAT GCCTGGAATG TGTTTGACTT TGTGACTGTG
    CTGGGCAGCA TCACTGACAT TTTAGTCACT GAGATTAACG AGCGTCAGCT GAACTTCAGT
    TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT
    CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA
    ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT
    TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT
    ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG
    AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT
    GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT
    CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT
    ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA
    AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG
    GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG
    ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG
    CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT
    AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC
    AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA
    CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG
    CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC
    AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA
    TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA
    GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG
    ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG
    TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA
    TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG
    CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT
    CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA
    TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA
    GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA
    GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA
    TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG
    CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA
    AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC
    AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG
    AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT
    ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG
    GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT
    CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC
    CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC
    AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299773.1
    CDS637..7770
    Translation

    Target ORF information:

    RefSeq Version XM_009301498.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301498.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CAGGAACCAA GGGCAAAGAC
    ATCAGCACTA TCAAGTCACT GAGGGTGTTG CGAGTCTTGC GACCTCTGAA GACCATCAAA
    CGTCTACCAA AACTGAAGGC CGTGTTTGAT TGTGTGGTGA ACTCCCTGAA GAATGTCCTG
    AATATCCTCA TTGTCTACAT TCTGTTCATG TTCATTTTCG CTGTCATTGC TGTGCAGCTC
    TTCAAGGGCA AGTTTTTTTA CTGCACAGAC GAGTCCAAGG GCCTGGAGAA AGACTGCAGG
    GGGCTGTTTT TGGATTATGA TAAGGATGAT GTGACGGCAC AACCGAGGGA ATGGAAGAAA
    TACGAGTTTC ACTATGACAA TGTGCTTTGG GCTTTTCTCA CTCTCTTCAC TGTTTCTACG
    GGAGAGGGCT GGCCTACGGT TTTGAAGCAC TCCGTGGATG CCACTTTTGA GGATCAGGGT
    CCCAGTCCAG GCTACCGCAT TGAGATGTCT ATTTTTTACG TGGTCTACTT TGTGGTTTTC
    CCTTTCTTCT TCGTCAACAT TTTTGTAGCC TTGATAATCA TCACATTCCA GGAACAGGGT
    GATAAGGTCT TGTCAGAGTG CAGTCTGGAG AAGAATGAGA GAGCCTGCAT CGACTTTGCT
    ATCAATGCCA AACCTCTGAC ACGCTACATG CCGCAGAACC AGCAGTCCTT TCAGTACCGC
    CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT GAGTACTCCA TCATGATCAT GATTGCTCTC
    AACACTGTGG TGCTCATGAT GAAGTTCCAT GGGGCGCCTG ACTTTTATGA GGCAATGCTT
    AAGTATCTCA ACATTGTCTT CACTGTTCTC TTTTCACTGG AGTGTATTCT CAAGATTATT
    GCTTTCGGCC CCTTGAATTA CCTGAAAGAT GCCTGGAATG TGTTTGACTT TGTGACTGTG
    CTGGGCAGCA TCACTGACAT TTTAGTCACT GAGATTAACG AGCGTCAGCT GAACTTCAGT
    TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT
    CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA
    ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT
    TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT
    ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG
    AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT
    GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT
    CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT
    ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA
    AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG
    GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG
    ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG
    CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT
    AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC
    AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA
    CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG
    CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC
    AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA
    TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA
    GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG
    ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG
    TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA
    TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG
    CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT
    CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA
    TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA
    GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA
    GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA
    TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG
    CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA
    AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC
    AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG
    AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT
    ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG
    GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT
    CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC
    CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC
    AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42263
    Clone ID Related Accession (Same CDS sequence) XM_009301499.2
    Accession Version XM_009301499.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCGAGTTATG TCTGCTCCCC TGCACATCAC TACTTGAGTA AAGAACAGCA GCGTTCTCTA
    AAAATGATGT CCGTGTGGGA GCAGCGCACC ACTCAGATCC GAAAGCACAT GCTAGCCAGC
    AGTGAGGCGT TGTACCAAGA AGAGCTCAAT CCACGGCTCA TGTCTAACCT TCCTCTACGC
    ACCGACATAA AAACCCACCT GGACCGGCCT TTGGTTGTGG AGCCTCGCTG TGATGGCCCC
    ATCAGCCCAC ACAGTGGCTG GGACAAACCC CAGGATTTGC AAGGCGAGGG CAGCACACCA
    GAAGAACGAG TCCCACCTGT ATTCGAGCCT CCACAGTCAC ATCCACCACG CAAGCACCAC
    CGTCACAGAG AGCGTCTTTC TAATGAGACC AATGAAAATG GAGACACGGG TCATGCCAAA
    GAAGGCCGGC ATCACGTTCA TCACAGCCGA AGTAAAGAGC ATGATGGGAC ACGTTGTAAG
    GAGGGGAAGG GTGACAGGAG CCGAAGCAGA GAAGGGGGAC GTAGACATCA CCATCAGAGC
    TCGGTGGATG AGGGCGTAGG AGGTGGAGAG AGGGAACATC GGCACCATCA CTCCCATAGG
    CATAACAGAG AGGGCAATGG TGTGGTCAGT GCTGGGGGGA AGAGTGAACG GCGGTCCAGG
    CAGAAAGAGG GCTCACGATC AGGGACAAGA GAGGGAGAGA GATGCTCAAG GGGGGAGAAT
    GGAGGAAATG GAGGGAGGAG GCGACACAGG CCACGAACCA GTAAAGCTCA GTCTACGCTG
    GAAGGAGAGG AGCAACATGT GAATGGAGAG GAGGAGGGCA AGTCGCAAAG CCATAGGCAC
    ATGGATATGG GAGGAGATTC AATGGCTGTA AACCCCCATC AACCTTCAGT TGGAAGCCGG
    TGGTGTCTTG AGAAACCAGA AGATTCGGAC AACAAACGAA ATATTGGTCA GACTGGAGGA
    ATGAGCACCC ATATACCCGT GACTGTCACT TCTCCCCCGG GAGAAACAAC CCTTATTCCC
    ATGAACAGTA TAGACAGCGA GACAGTGCCC ATGACTGAGA AGAATCTAGA AGATCTAAAT
    CAAAGCAGCA TTCGTCCCAT CCTCCCCTTC AGCTCCATGT TCATTTTCAA CCCAACTAAT
    CCGGTGAGAC GTTTATGCCA CTACATTGTG AGTCTGCGCT ATTTTGAGAT GTGTATCCTG
    GTGGTCATTG CCATGAGCAG TATAGCCCTG GCTGCAGAAG ACCCGGTCCA AGCCAATGCA
    CCTCGGAACA ATGTCCTGAA GTATTTGGAC TATGTCTTCA CTGGTGTTTT CACATTCGAG
    ATGGTCATTA AGATGGTGGA TTTAGGTTTA ATTCTGCATC CTGGTTCCTA CTTTCGAGAT
    CTATGGAATA TTTTGGACTT CATTGTGGTC AGTGGTGCAC TGGTGGCATT CGCATTCTCG
    AGCTTCATGG GATCACATCA AAGCGTAAAC AGAGGAACCA AGGGCAAAGA CATCAGCACT
    ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG CGACCTCTGA AGACCATCAA ACGTCTACCA
    AAACTGAAGG CCGTGTTTGA TTGTGTGGTG AACTCCCTGA AGAATGTCCT GAATATCCTC
    ATTGTCTACA TTCTGTTCAT GTTCATTTTC GCTGTCATTG CTGTGCAGCT CTTCAAGGGC
    AAGTTTTTTT ACTGCACAGA CGAGTCCAAG GGCCTGGAGA AAGACTGCAG GGGGCTGTTT
    TTGGATTATG ATAAGGATGA TGTGACGGCA CAACCGAGGG AATGGAAGAA ATACGAGTTT
    CACTATGACA ATGTGCTTTG GGCTTTTCTC ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC
    TGGCCTACGG TTTTGAAGCA CTCCGTGGAT GCCACTTTTG AGGATCAGGG TCCCAGTCCA
    GGCTACCGCA TTGAGATGTC TATTTTTTAC GTGGTCTACT TTGTGGTTTT CCCTTTCTTC
    TTCGTCAACA TTTTTGTAGC CTTGATAATC ATCACATTCC AGGAACAGGG TGATAAGGTC
    TTGTCAGAGT GCAGTCTGGA GAAGAATGAG AGAGCCTGCA TCGACTTTGC TATCAATGCC
    AAACCTCTGA CACGCTACAT GCCGCAGAAC CAGCAGTCCT TTCAGTACCG CCTGTGGAAG
    TTTGTGGTCT CTCCGCCGTT TGAGTACTCC ATCATGATCA TGATTGCTCT CAACACTGTG
    GTGCTCATGA TGAAGTTCCA TGGGGCGCCT GACTTTTATG AGGCAATGCT TAAGTATCTC
    AACATTGTCT TCACTGTTCT CTTTTCACTG GAGTGTATTC TCAAGATTAT TGCTTTCGGC
    CCCTTGAATT ACCTGAAAGA TGCCTGGAAT GTGTTTGACT TTGTGACTGT GCTGGGCAGC
    ATCACTGACA TTTTAGTCAC TGAGATTAAC ACAACGGAGC GTCAGCTGAA CTTCAGTTTC
    CTGAGGCTCT TTAGAGCAGC CCGACTCATC AAGCTCCTCC GGCAGGGATA CACCATTCGC
    ATCCTGCTCT GGACATTTGT CCAGTCTTTT AAGGCACTGC CTTATGTTTG CCTTCTAATC
    GCCATGCTGT TTTTCATCTA CGCCATTATT GGGATGCAGG TGTTTGGAAA CATTGATTTG
    AATGATGACA CGGCAATCAA CCGTCACAAC AACTTCCGCA CTTTCCTCCA AGCTCTTATG
    CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT TGGCATGATA TCATGCTGTC GTGTCTGAGC
    GAGCGCACCT GTGATCCGAG CTCAGGCACC CTTGGAAAGG AGTGTGGGAG TGACTTTGCC
    TACTTCTACT TTGTCTCCTT CATTTTCCTC TGCTCATTTC TGATGTTGAA CCTGTTTGTG
    GCCGTCATTA TGGACAACTT TGAATATCTG ACACGCGACT CCTCCATCCT GGGCCCTCAC
    CACCTGGATG AGTTTATTCG AGTGTGGGCG GAGTATGACC CAGCCGCATG TGGCCGTATC
    AAGTACCTCG ATATGTATGA GATGCTGCTC CACATGTCCC CACCTTTAGG TTTGGGAAAG
    AAATGTCCTC CACGAATCGC CTACAAGCGT CTGGTGAAAA TGAACATGCC CATTGCGGAT
    GACAACAGTG TACACTTTAC CTCAACGCTG ATGGCTCTGA TCCGTACTGC CCTGGAGATC
    AAACTGGCTT CAGGCATGGT GGCTCAGCGT CTGTCAGATG CTGAACTGAA GAAGGAGCTC
    TCTACAGTTT GGCCAAACCT GTCTCAGAAG ACCATGGACC TGCTGGTCAC TCCACATAAA
    CCTAATGAGC TCACAGTGGG GAAGGTGTAT GCAGCTCTTA TGATCTTTGA CTATTACAAG
    CAGAACAGAG CAAAAAGACT TCAGATGCAG CAACAGCAGC TGAAAGAACA GGCAGGACCT
    GGATCTCAGA ATAAAGTCAG CGCTCTCTTA GAGCCCATTC TCCCACTAAC CCACATGCAA
    GATCAGCCAG TGAACGGTGT CGATTCAGCT TCAGAGTGTC AAGCTCAGAC CAGACCCAGC
    TCCACCACAC TCAACAATGG ACGCACAACT CTTGACAACA CAATAAAAGG GTCTTCATCC
    TGGGCCTCAG AGAAATCCAA GGAAGTCCAA AAGTCTAAAC GGAGACCAAT GTCTAGAGGT
    CAATCTGAGG ATGCCTCAAA TGCAAACACT GCCCAGGAGT CAGTAGAAAT GAGAAAGATG
    GAGAACAGTG CCAACACAGT GACCTCTGGT GGCCTTGAGG GCCAAGGACG TGCAGCGTCC
    ATGCCAAAAC TCAATGCTGA GATGCAGAGG AGTCATTCCC GTCAATCTCC TGGAACATTA
    CTGGAACCCA TTCCAGATTC CCCCATGAGG CGCTCTGCAT CCACTTTTGC ACCACAGCGC
    CCTCACGAGG TGAATCTGAA TGACTACATT CTGGAGAAAC CTGTTCAGGA ACGCCATCAC
    CACCATCACC ATCATCACCG ATGCCATCAT CGCCGGGACA GAGACAAGAA ACAGAGATCT
    CTGGACAGGT CGCCTACTGG CCATCACAAA ACTACAGGCA CAGCCACTGA CTCTGTAGAG
    GAGAGGGCAC ATGACCGGGG TCGTTCCCAT GAGAGGAAGC ACCATTCATC TTCAGGAGAG
    AAGCAGCGGT ATTACTCGTG TGATCGCTAT GCCAGTCGGG AACACTGTTC CAGCAAATCT
    GCCGTGGCAA GCTGTGCCAC CTCACCCAGC GAGACCCAGG AGGCCAATCT TAACAAGCAG
    GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG TTGTTGACTT CAGGGGCGAG CACTCCAAGC
    CGCGGGCGCA GACAGCTGCC CCAGACCCCC CTCACTCCTC GTCCCTCCGT GGCCTACAGG
    ACTGCCAACT CTTCCCCTGT GCAACTTCTG GCCACAACGA CCAGCTTGCT TCCCCCGAGT
    CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG CACAACGCTC TTTTACTCAG CGGCTCTATC
    TCCAGCTCGC CGGCCCCCGT CAGTCGCATT AACTCAGAAC CCTTCTTGGG GCCCCCGGAC
    CACCCTGCTG GCTTGCAGCC CGAAGCCTTC GCTGTCTTCC AAGAGAATCC GAGCCCTCAG
    ATGGGCCGTT CTCCTCAGAC TGTAGGGATG CCCTTCGTGG CGATCCCCCC ACCTCCCCCA
    CAGCAGTTTC GCAGGGTCCC CAACGGGTAC CACTTCTCCA CGGGCCCCAG CGCCAGCAGA
    GGACGGGCAC AGCAGTACTA CCCAGAGGCA GAGGTGGAGG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299774.1
    CDS636..7766
    Translation

    Target ORF information:

    RefSeq Version XM_009301499.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301499.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCGAGTTATG TCTGCTCCCC TGCACATCAC TACTTGAGTA AAGAACAGCA GCGTTCTCTA
    AAAATGATGT CCGTGTGGGA GCAGCGCACC ACTCAGATCC GAAAGCACAT GCTAGCCAGC
    AGTGAGGCGT TGTACCAAGA AGAGCTCAAT CCACGGCTCA TGTCTAACCT TCCTCTACGC
    ACCGACATAA AAACCCACCT GGACCGGCCT TTGGTTGTGG AGCCTCGCTG TGATGGCCCC
    ATCAGCCCAC ACAGTGGCTG GGACAAACCC CAGGATTTGC AAGGCGAGGG CAGCACACCA
    GAAGAACGAG TCCCACCTGT ATTCGAGCCT CCACAGTCAC ATCCACCACG CAAGCACCAC
    CGTCACAGAG AGCGTCTTTC TAATGAGACC AATGAAAATG GAGACACGGG TCATGCCAAA
    GAAGGCCGGC ATCACGTTCA TCACAGCCGA AGTAAAGAGC ATGATGGGAC ACGTTGTAAG
    GAGGGGAAGG GTGACAGGAG CCGAAGCAGA GAAGGGGGAC GTAGACATCA CCATCAGAGC
    TCGGTGGATG AGGGCGTAGG AGGTGGAGAG AGGGAACATC GGCACCATCA CTCCCATAGG
    CATAACAGAG AGGGCAATGG TGTGGTCAGT GCTGGGGGGA AGAGTGAACG GCGGTCCAGG
    CAGAAAGAGG GCTCACGATC AGGGACAAGA GAGGGAGAGA GATGCTCAAG GGGGGAGAAT
    GGAGGAAATG GAGGGAGGAG GCGACACAGG CCACGAACCA GTAAAGCTCA GTCTACGCTG
    GAAGGAGAGG AGCAACATGT GAATGGAGAG GAGGAGGGCA AGTCGCAAAG CCATAGGCAC
    ATGGATATGG GAGGAGATTC AATGGCTGTA AACCCCCATC AACCTTCAGT TGGAAGCCGG
    TGGTGTCTTG AGAAACCAGA AGATTCGGAC AACAAACGAA ATATTGGTCA GACTGGAGGA
    ATGAGCACCC ATATACCCGT GACTGTCACT TCTCCCCCGG GAGAAACAAC CCTTATTCCC
    ATGAACAGTA TAGACAGCGA GACAGTGCCC ATGACTGAGA AGAATCTAGA AGATCTAAAT
    CAAAGCAGCA TTCGTCCCAT CCTCCCCTTC AGCTCCATGT TCATTTTCAA CCCAACTAAT
    CCGGTGAGAC GTTTATGCCA CTACATTGTG AGTCTGCGCT ATTTTGAGAT GTGTATCCTG
    GTGGTCATTG CCATGAGCAG TATAGCCCTG GCTGCAGAAG ACCCGGTCCA AGCCAATGCA
    CCTCGGAACA ATGTCCTGAA GTATTTGGAC TATGTCTTCA CTGGTGTTTT CACATTCGAG
    ATGGTCATTA AGATGGTGGA TTTAGGTTTA ATTCTGCATC CTGGTTCCTA CTTTCGAGAT
    CTATGGAATA TTTTGGACTT CATTGTGGTC AGTGGTGCAC TGGTGGCATT CGCATTCTCG
    AGCTTCATGG GATCACATCA AAGCGTAAAC AGAGGAACCA AGGGCAAAGA CATCAGCACT
    ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG CGACCTCTGA AGACCATCAA ACGTCTACCA
    AAACTGAAGG CCGTGTTTGA TTGTGTGGTG AACTCCCTGA AGAATGTCCT GAATATCCTC
    ATTGTCTACA TTCTGTTCAT GTTCATTTTC GCTGTCATTG CTGTGCAGCT CTTCAAGGGC
    AAGTTTTTTT ACTGCACAGA CGAGTCCAAG GGCCTGGAGA AAGACTGCAG GGGGCTGTTT
    TTGGATTATG ATAAGGATGA TGTGACGGCA CAACCGAGGG AATGGAAGAA ATACGAGTTT
    CACTATGACA ATGTGCTTTG GGCTTTTCTC ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC
    TGGCCTACGG TTTTGAAGCA CTCCGTGGAT GCCACTTTTG AGGATCAGGG TCCCAGTCCA
    GGCTACCGCA TTGAGATGTC TATTTTTTAC GTGGTCTACT TTGTGGTTTT CCCTTTCTTC
    TTCGTCAACA TTTTTGTAGC CTTGATAATC ATCACATTCC AGGAACAGGG TGATAAGGTC
    TTGTCAGAGT GCAGTCTGGA GAAGAATGAG AGAGCCTGCA TCGACTTTGC TATCAATGCC
    AAACCTCTGA CACGCTACAT GCCGCAGAAC CAGCAGTCCT TTCAGTACCG CCTGTGGAAG
    TTTGTGGTCT CTCCGCCGTT TGAGTACTCC ATCATGATCA TGATTGCTCT CAACACTGTG
    GTGCTCATGA TGAAGTTCCA TGGGGCGCCT GACTTTTATG AGGCAATGCT TAAGTATCTC
    AACATTGTCT TCACTGTTCT CTTTTCACTG GAGTGTATTC TCAAGATTAT TGCTTTCGGC
    CCCTTGAATT ACCTGAAAGA TGCCTGGAAT GTGTTTGACT TTGTGACTGT GCTGGGCAGC
    ATCACTGACA TTTTAGTCAC TGAGATTAAC ACAACGGAGC GTCAGCTGAA CTTCAGTTTC
    CTGAGGCTCT TTAGAGCAGC CCGACTCATC AAGCTCCTCC GGCAGGGATA CACCATTCGC
    ATCCTGCTCT GGACATTTGT CCAGTCTTTT AAGGCACTGC CTTATGTTTG CCTTCTAATC
    GCCATGCTGT TTTTCATCTA CGCCATTATT GGGATGCAGG TGTTTGGAAA CATTGATTTG
    AATGATGACA CGGCAATCAA CCGTCACAAC AACTTCCGCA CTTTCCTCCA AGCTCTTATG
    CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT TGGCATGATA TCATGCTGTC GTGTCTGAGC
    GAGCGCACCT GTGATCCGAG CTCAGGCACC CTTGGAAAGG AGTGTGGGAG TGACTTTGCC
    TACTTCTACT TTGTCTCCTT CATTTTCCTC TGCTCATTTC TGATGTTGAA CCTGTTTGTG
    GCCGTCATTA TGGACAACTT TGAATATCTG ACACGCGACT CCTCCATCCT GGGCCCTCAC
    CACCTGGATG AGTTTATTCG AGTGTGGGCG GAGTATGACC CAGCCGCATG TGGCCGTATC
    AAGTACCTCG ATATGTATGA GATGCTGCTC CACATGTCCC CACCTTTAGG TTTGGGAAAG
    AAATGTCCTC CACGAATCGC CTACAAGCGT CTGGTGAAAA TGAACATGCC CATTGCGGAT
    GACAACAGTG TACACTTTAC CTCAACGCTG ATGGCTCTGA TCCGTACTGC CCTGGAGATC
    AAACTGGCTT CAGGCATGGT GGCTCAGCGT CTGTCAGATG CTGAACTGAA GAAGGAGCTC
    TCTACAGTTT GGCCAAACCT GTCTCAGAAG ACCATGGACC TGCTGGTCAC TCCACATAAA
    CCTAATGAGC TCACAGTGGG GAAGGTGTAT GCAGCTCTTA TGATCTTTGA CTATTACAAG
    CAGAACAGAG CAAAAAGACT TCAGATGCAG CAACAGCAGC TGAAAGAACA GGCAGGACCT
    GGATCTCAGA ATAAAGTCAG CGCTCTCTTA GAGCCCATTC TCCCACTAAC CCACATGCAA
    GATCAGCCAG TGAACGGTGT CGATTCAGCT TCAGAGTGTC AAGCTCAGAC CAGACCCAGC
    TCCACCACAC TCAACAATGG ACGCACAACT CTTGACAACA CAATAAAAGG GTCTTCATCC
    TGGGCCTCAG AGAAATCCAA GGAAGTCCAA AAGTCTAAAC GGAGACCAAT GTCTAGAGGT
    CAATCTGAGG ATGCCTCAAA TGCAAACACT GCCCAGGAGT CAGTAGAAAT GAGAAAGATG
    GAGAACAGTG CCAACACAGT GACCTCTGGT GGCCTTGAGG GCCAAGGACG TGCAGCGTCC
    ATGCCAAAAC TCAATGCTGA GATGCAGAGG AGTCATTCCC GTCAATCTCC TGGAACATTA
    CTGGAACCCA TTCCAGATTC CCCCATGAGG CGCTCTGCAT CCACTTTTGC ACCACAGCGC
    CCTCACGAGG TGAATCTGAA TGACTACATT CTGGAGAAAC CTGTTCAGGA ACGCCATCAC
    CACCATCACC ATCATCACCG ATGCCATCAT CGCCGGGACA GAGACAAGAA ACAGAGATCT
    CTGGACAGGT CGCCTACTGG CCATCACAAA ACTACAGGCA CAGCCACTGA CTCTGTAGAG
    GAGAGGGCAC ATGACCGGGG TCGTTCCCAT GAGAGGAAGC ACCATTCATC TTCAGGAGAG
    AAGCAGCGGT ATTACTCGTG TGATCGCTAT GCCAGTCGGG AACACTGTTC CAGCAAATCT
    GCCGTGGCAA GCTGTGCCAC CTCACCCAGC GAGACCCAGG AGGCCAATCT TAACAAGCAG
    GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG TTGTTGACTT CAGGGGCGAG CACTCCAAGC
    CGCGGGCGCA GACAGCTGCC CCAGACCCCC CTCACTCCTC GTCCCTCCGT GGCCTACAGG
    ACTGCCAACT CTTCCCCTGT GCAACTTCTG GCCACAACGA CCAGCTTGCT TCCCCCGAGT
    CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG CACAACGCTC TTTTACTCAG CGGCTCTATC
    TCCAGCTCGC CGGCCCCCGT CAGTCGCATT AACTCAGAAC CCTTCTTGGG GCCCCCGGAC
    CACCCTGCTG GCTTGCAGCC CGAAGCCTTC GCTGTCTTCC AAGAGAATCC GAGCCCTCAG
    ATGGGCCGTT CTCCTCAGAC TGTAGGGATG CCCTTCGTGG CGATCCCCCC ACCTCCCCCA
    CAGCAGTTTC GCAGGGTCCC CAACGGGTAC CACTTCTCCA CGGGCCCCAG CGCCAGCAGA
    GGACGGGCAC AGCAGTACTA CCCAGAGGCA GAGGTGGAGG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42264
    Clone ID Related Accession (Same CDS sequence) XM_009301500.2 , XM_009301500.3
    Accession Version XM_009301500.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC
    ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT
    TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT
    ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC
    AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG
    GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC
    ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC
    CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT
    CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG
    GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG
    CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG
    CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG
    CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG
    AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG
    TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG
    CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC
    TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA
    GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA
    ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC
    CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC
    ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT
    GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG
    AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG
    AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC
    ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT
    CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC
    AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC
    CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC
    TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC
    AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG
    GTGTTGTTGA CTTCAGGGGC GAGCACTCCA AGCCGCGGGC GCAGACAGCT GCCCCAGACC
    CCCCTCACTC CTCGTCCCTC CGTGGCCTAC AGGACTGCCA ACTCTTCCCC TGTGCAACTT
    CTGGCCACAA CGACCAGCTT GCTTCCCCCG AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC
    GAGCACAACG CTCTTTTACT CAGCGGCTCT ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC
    ATTAACTCAG AACCCTTCTT GGGGCCCCCG GACCACCCTG CTGGCTTGCA GCCCGAAGCC
    TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT CAGATGGGCC GTTCTCCTCA GACTGTAGGG
    ATGCCCTTCG TGGCGATCCC CCCACCTCCC CCACAGCAGT TTCGCAGGGT CCCCAACGGG
    TACCACTTCT CCACGGGCCC CAGCGCCAGC AGAGGACGGG CACAGCAGTA CTACCCAGAG
    GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299775.1
    CDS636..7739
    Translation

    Target ORF information:

    RefSeq Version XM_009301500.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301500.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC
    ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT
    TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT
    ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC
    AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG
    GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC
    ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC
    CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT
    CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG
    GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG
    CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG
    CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG
    CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG
    AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG
    TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG
    CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC
    TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA
    GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA
    ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC
    CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC
    ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT
    GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG
    AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG
    AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC
    ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT
    CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC
    AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC
    CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC
    TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC
    AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG
    GTGTTGTTGA CTTCAGGGGC GAGCACTCCA AGCCGCGGGC GCAGACAGCT GCCCCAGACC
    CCCCTCACTC CTCGTCCCTC CGTGGCCTAC AGGACTGCCA ACTCTTCCCC TGTGCAACTT
    CTGGCCACAA CGACCAGCTT GCTTCCCCCG AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC
    GAGCACAACG CTCTTTTACT CAGCGGCTCT ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC
    ATTAACTCAG AACCCTTCTT GGGGCCCCCG GACCACCCTG CTGGCTTGCA GCCCGAAGCC
    TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT CAGATGGGCC GTTCTCCTCA GACTGTAGGG
    ATGCCCTTCG TGGCGATCCC CCCACCTCCC CCACAGCAGT TTCGCAGGGT CCCCAACGGG
    TACCACTTCT CCACGGGCCC CAGCGCCAGC AGAGGACGGG CACAGCAGTA CTACCCAGAG
    GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42264
    Clone ID Related Accession (Same CDS sequence) XM_009301500.2 , XM_009301500.3
    Accession Version XM_009301500.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    841
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2641
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    3181
    3241
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    4141
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    5281
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    5401
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    5521
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    5701
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    5941
    6001
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    6181
    6241
    6301
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    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC
    ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT
    TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT
    ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC
    AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG
    GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC
    ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC
    CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT
    CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG
    GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG
    CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG
    CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG
    CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG
    AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG
    TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG
    CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC
    TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA
    GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA
    ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC
    CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC
    ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT
    GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG
    AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG
    AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC
    ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT
    CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC
    AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC
    CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC
    TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC
    AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG
    GTGTTGTTGA CTTCAGGGGC GAGCACTCCA AGCCGCGGGC GCAGACAGCT GCCCCAGACC
    CCCCTCACTC CTCGTCCCTC CGTGGCCTAC AGGACTGCCA ACTCTTCCCC TGTGCAACTT
    CTGGCCACAA CGACCAGCTT GCTTCCCCCG AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC
    GAGCACAACG CTCTTTTACT CAGCGGCTCT ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC
    ATTAACTCAG AACCCTTCTT GGGGCCCCCG GACCACCCTG CTGGCTTGCA GCCCGAAGCC
    TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT CAGATGGGCC GTTCTCCTCA GACTGTAGGG
    ATGCCCTTCG TGGCGATCCC CCCACCTCCC CCACAGCAGT TTCGCAGGGT CCCCAACGGG
    TACCACTTCT CCACGGGCCC CAGCGCCAGC AGAGGACGGG CACAGCAGTA CTACCCAGAG
    GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299775.1
    CDS1..7104
    Translation

    Target ORF information:

    RefSeq Version XM_009301500.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301500.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC
    ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT
    TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT
    ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC
    AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG
    GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC
    ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC
    CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT
    CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG
    GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG
    CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG
    CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG
    CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG
    AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG
    TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG
    CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC
    TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA
    GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA
    ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC
    CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC
    ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT
    GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG
    AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG
    AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC
    ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT
    CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC
    AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC
    CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC
    TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC
    AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG
    GTGTTGTTGA CTTCAGGGGC GAGCACTCCA AGCCGCGGGC GCAGACAGCT GCCCCAGACC
    CCCCTCACTC CTCGTCCCTC CGTGGCCTAC AGGACTGCCA ACTCTTCCCC TGTGCAACTT
    CTGGCCACAA CGACCAGCTT GCTTCCCCCG AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC
    GAGCACAACG CTCTTTTACT CAGCGGCTCT ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC
    ATTAACTCAG AACCCTTCTT GGGGCCCCCG GACCACCCTG CTGGCTTGCA GCCCGAAGCC
    TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT CAGATGGGCC GTTCTCCTCA GACTGTAGGG
    ATGCCCTTCG TGGCGATCCC CCCACCTCCC CCACAGCAGT TTCGCAGGGT CCCCAACGGG
    TACCACTTCT CCACGGGCCC CAGCGCCAGC AGAGGACGGG CACAGCAGTA CTACCCAGAG
    GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42265
    Clone ID Related Accession (Same CDS sequence) XM_009301501.3 , XM_009301501.2
    Accession Version XM_009301501.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC
    ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA
    CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC
    CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG
    GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG
    TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG
    TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG
    GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT
    CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC
    TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG
    GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT
    GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG
    AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT
    GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT
    CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC
    GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC
    AGCATCACTG ACATTTTAGT CACTGAGATT AACACAACGG AGCGTCAGCT GAACTTCAGT
    TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT
    CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA
    ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT
    TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT
    ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG
    AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT
    GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT
    CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT
    ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA
    AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG
    GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG
    ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG
    CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT
    AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC
    AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA
    CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG
    CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC
    AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA
    TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA
    GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG
    ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG
    TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA
    TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG
    CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT
    CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA
    TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA
    GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA
    GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA
    TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG
    CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA
    AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC
    AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG
    AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT
    ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG
    GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT
    CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC
    CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC
    AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299776.1
    CDS1..7074
    Translation

    Target ORF information:

    RefSeq Version XM_009301501.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301501.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC
    ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA
    CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC
    CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG
    GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG
    TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG
    TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG
    GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT
    CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC
    TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG
    GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT
    GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG
    AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT
    GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT
    CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC
    GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC
    AGCATCACTG ACATTTTAGT CACTGAGATT AACACAACGG AGCGTCAGCT GAACTTCAGT
    TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT
    CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA
    ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT
    TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT
    ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG
    AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT
    GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT
    CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT
    ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA
    AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG
    GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG
    ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG
    CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT
    AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC
    AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA
    CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG
    CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC
    AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA
    TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA
    GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG
    ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG
    TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA
    TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG
    CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT
    CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA
    TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA
    GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA
    GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA
    TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG
    CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA
    AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC
    AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG
    AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT
    ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG
    GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT
    CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC
    CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC
    AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42265
    Clone ID Related Accession (Same CDS sequence) XM_009301501.3 , XM_009301501.2
    Accession Version XM_009301501.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC
    ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA
    CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC
    CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG
    GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG
    TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG
    TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG
    GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT
    CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC
    TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG
    GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT
    GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG
    AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT
    GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT
    CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC
    GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC
    AGCATCACTG ACATTTTAGT CACTGAGATT AACACAACGG AGCGTCAGCT GAACTTCAGT
    TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT
    CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA
    ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT
    TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT
    ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG
    AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT
    GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT
    CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT
    ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA
    AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG
    GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG
    ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG
    CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT
    AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC
    AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA
    CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG
    CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC
    AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA
    TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA
    GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG
    ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG
    TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA
    TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG
    CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT
    CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA
    TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA
    GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA
    GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA
    TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG
    CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA
    AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC
    AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG
    AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT
    ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG
    GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT
    CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC
    CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC
    AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299776.1
    CDS636..7709
    Translation

    Target ORF information:

    RefSeq Version XM_009301501.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301501.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT
    CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC
    AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA
    CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC
    CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA
    CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC
    CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC
    AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT
    AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG
    AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT
    AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC
    AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG
    AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG
    CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG
    CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC
    CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA
    GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT
    CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA
    AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT
    AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC
    CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT
    GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC
    GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA
    GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC
    TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC
    ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA
    CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC
    CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG
    GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG
    TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG
    TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG
    GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT
    CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC
    TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG
    GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT
    GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG
    AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT
    GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT
    CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC
    GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC
    AGCATCACTG ACATTTTAGT CACTGAGATT AACACAACGG AGCGTCAGCT GAACTTCAGT
    TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT
    CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA
    ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT
    TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT
    ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG
    AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT
    GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT
    CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT
    ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA
    AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG
    GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG
    ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG
    CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT
    AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC
    AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA
    CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG
    CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC
    AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA
    TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA
    GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG
    ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG
    TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA
    TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG
    CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT
    CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA
    TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA
    GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA
    GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA
    TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG
    CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA
    AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC
    AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG
    AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT
    ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG
    GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT
    CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC
    CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC
    AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42266
    Clone ID Related Accession (Same CDS sequence) XM_009301503.2
    Accession Version XM_009301503.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT
    CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG
    TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT
    GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC
    ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA
    GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT
    GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT
    TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG
    CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG
    TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA
    GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG
    GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC
    ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG
    TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC
    CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT
    CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA
    GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC
    ACTGAGATTA ACACAACGGA GCGTCAGCTG AACTTCAGTT TCCTGAGGCT CTTTAGAGCA
    GCCCGACTCA TCAAGCTCCT CCGGCAGGGA TACACCATTC GCATCCTGCT CTGGACATTT
    GTCCAGTCTT TTAAGGCACT GCCTTATGTT TGCCTTCTAA TCGCCATGCT GTTTTTCATC
    TACGCCATTA TTGGGATGCA GGTGTTTGGA AACATTGATT TGAATGATGA CACGGCAATC
    AACCGTCACA ACAACTTCCG CACTTTCCTC CAAGCTCTTA TGCTTTTGTT CAGGAGTGCC
    ACTGGCGAGG CTTGGCATGA TATCATGCTG TCGTGTCTGA GCGAGCGCAC CTGTGATCCG
    AGCTCAGGCA CCCTTGGAAA GGAGTGTGGG AGTGACTTTG CCTACTTCTA CTTTGTCTCC
    TTCATTTTCC TCTGCTCATT TCTGATGTTG AACCTGTTTG TGGCCGTCAT TATGGACAAC
    TTTGAATATC TGACACGCGA CTCCTCCATC CTGGGCCCTC ACCACCTGGA TGAGTTTATT
    CGAGTGTGGG CGGAGTATGA CCCAGCCGCA TGTGGCCGTA TCAAGTACCT CGATATGTAT
    GAGATGCTGC TCCACATGTC CCCACCTTTA GGTTTGGGAA AGAAATGTCC TCCACGAATC
    GCCTACAAGC GTCTGGTGAA AATGAACATG CCCATTGCGG ATGACAACAG TGTACACTTT
    ACCTCAACGC TGATGGCTCT GATCCGTACT GCCCTGGAGA TCAAACTGGC TTCAGGCATG
    GTGGCTCAGC GTCTGTCAGA TGCTGAACTG AAGAAGGAGC TCTCTACAGT TTGGCCAAAC
    CTGTCTCAGA AGACCATGGA CCTGCTGGTC ACTCCACATA AACCTAATGA GCTCACAGTG
    GGGAAGGTGT ATGCAGCTCT TATGATCTTT GACTATTACA AGCAGAACAG AGCAAAAAGA
    CTTCAGATGC AGCAACAGCA GCTGAAAGAA CAGGCAGGAC CTGGATCTCA GAATAAAGTC
    AGCGCTCTCT TAGAGCCCAT TCTCCCACTA ACCCACATGC AAGATCAGCC AGTGAACGGT
    GTCGATTCAG CTTCAGAGTG TCAAGCTCAG ACCAGACCCA GCTCCACCAC ACTCAACAAT
    GGACGCACAA CTCTTGACAA CACAATAAAA GGGTCTTCAT CCTGGGCCTC AGAGAAATCC
    AAGGAAGTCC AAAAGTCTAA ACGGAGACCA ATGTCTAGAG GTCAATCTGA GGATGCCTCA
    AATGCAAACA CTGCCCAGGA GTCAGTAGAA ATGAGAAAGA TGGAGAACAG TGCCAACACA
    GTGACCTCTG GTGGCCTTGA GGGCCAAGGA CGTGCAGCGT CCATGCCAAA ACTCAATGCT
    GAGATGCAGA GGAGTCATTC CCGTCAATCT CCTGGAACAT TACTGGAACC CATTCCAGAT
    TCCCCCATGA GGCGCTCTGC ATCCACTTTT GCACCACAGC GCCCTCACGA GGTGAATCTG
    AATGACTACA TTCTGGAGAA ACCTGTTCAG GAACGCCATC ACCACCATCA CCATCATCAC
    CGATGCCATC ATCGCCGGGA CAGAGACAAG AAACAGAGAT CTCTGGACAG GTCGCCTACT
    GGCCATCACA AAACTACAGG CACAGCCACT GACTCTGTAG AGGAGAGGGC ACATGACCGG
    GGTCGTTCCC ATGAGAGGAA GCACCATTCA TCTTCAGGAG AGAAGCAGCG GTATTACTCG
    TGTGATCGCT ATGCCAGTCG GGAACACTGT TCCAGCAAAT CTGCCGTGGC AAGCTGTGCC
    ACCTCACCCA GCGAGACCCA GGAGGCCAAT CTTAACAAGC AGTGGGTGGG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299778.1
    CDS639..7292
    Translation

    Target ORF information:

    RefSeq Version XM_009301503.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301503.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC
    CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG
    GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA
    GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC
    CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC
    TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT
    GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT
    TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT
    CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG
    AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA
    GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT
    GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA
    TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG
    AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT
    GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT
    TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA
    GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC
    GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC
    GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC
    ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC
    CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC
    AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG
    AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG
    GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC
    TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT
    CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG
    TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT
    GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC
    ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA
    GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT
    GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT
    TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG
    CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG
    TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA
    GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG
    GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC
    ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG
    TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC
    CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT
    CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA
    GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC
    ACTGAGATTA ACACAACGGA GCGTCAGCTG AACTTCAGTT TCCTGAGGCT CTTTAGAGCA
    GCCCGACTCA TCAAGCTCCT CCGGCAGGGA TACACCATTC GCATCCTGCT CTGGACATTT
    GTCCAGTCTT TTAAGGCACT GCCTTATGTT TGCCTTCTAA TCGCCATGCT GTTTTTCATC
    TACGCCATTA TTGGGATGCA GGTGTTTGGA AACATTGATT TGAATGATGA CACGGCAATC
    AACCGTCACA ACAACTTCCG CACTTTCCTC CAAGCTCTTA TGCTTTTGTT CAGGAGTGCC
    ACTGGCGAGG CTTGGCATGA TATCATGCTG TCGTGTCTGA GCGAGCGCAC CTGTGATCCG
    AGCTCAGGCA CCCTTGGAAA GGAGTGTGGG AGTGACTTTG CCTACTTCTA CTTTGTCTCC
    TTCATTTTCC TCTGCTCATT TCTGATGTTG AACCTGTTTG TGGCCGTCAT TATGGACAAC
    TTTGAATATC TGACACGCGA CTCCTCCATC CTGGGCCCTC ACCACCTGGA TGAGTTTATT
    CGAGTGTGGG CGGAGTATGA CCCAGCCGCA TGTGGCCGTA TCAAGTACCT CGATATGTAT
    GAGATGCTGC TCCACATGTC CCCACCTTTA GGTTTGGGAA AGAAATGTCC TCCACGAATC
    GCCTACAAGC GTCTGGTGAA AATGAACATG CCCATTGCGG ATGACAACAG TGTACACTTT
    ACCTCAACGC TGATGGCTCT GATCCGTACT GCCCTGGAGA TCAAACTGGC TTCAGGCATG
    GTGGCTCAGC GTCTGTCAGA TGCTGAACTG AAGAAGGAGC TCTCTACAGT TTGGCCAAAC
    CTGTCTCAGA AGACCATGGA CCTGCTGGTC ACTCCACATA AACCTAATGA GCTCACAGTG
    GGGAAGGTGT ATGCAGCTCT TATGATCTTT GACTATTACA AGCAGAACAG AGCAAAAAGA
    CTTCAGATGC AGCAACAGCA GCTGAAAGAA CAGGCAGGAC CTGGATCTCA GAATAAAGTC
    AGCGCTCTCT TAGAGCCCAT TCTCCCACTA ACCCACATGC AAGATCAGCC AGTGAACGGT
    GTCGATTCAG CTTCAGAGTG TCAAGCTCAG ACCAGACCCA GCTCCACCAC ACTCAACAAT
    GGACGCACAA CTCTTGACAA CACAATAAAA GGGTCTTCAT CCTGGGCCTC AGAGAAATCC
    AAGGAAGTCC AAAAGTCTAA ACGGAGACCA ATGTCTAGAG GTCAATCTGA GGATGCCTCA
    AATGCAAACA CTGCCCAGGA GTCAGTAGAA ATGAGAAAGA TGGAGAACAG TGCCAACACA
    GTGACCTCTG GTGGCCTTGA GGGCCAAGGA CGTGCAGCGT CCATGCCAAA ACTCAATGCT
    GAGATGCAGA GGAGTCATTC CCGTCAATCT CCTGGAACAT TACTGGAACC CATTCCAGAT
    TCCCCCATGA GGCGCTCTGC ATCCACTTTT GCACCACAGC GCCCTCACGA GGTGAATCTG
    AATGACTACA TTCTGGAGAA ACCTGTTCAG GAACGCCATC ACCACCATCA CCATCATCAC
    CGATGCCATC ATCGCCGGGA CAGAGACAAG AAACAGAGAT CTCTGGACAG GTCGCCTACT
    GGCCATCACA AAACTACAGG CACAGCCACT GACTCTGTAG AGGAGAGGGC ACATGACCGG
    GGTCGTTCCC ATGAGAGGAA GCACCATTCA TCTTCAGGAG AGAAGCAGCG GTATTACTCG
    TGTGATCGCT ATGCCAGTCG GGAACACTGT TCCAGCAAAT CTGCCGTGGC AAGCTGTGCC
    ACCTCACCCA GCGAGACCCA GGAGGCCAAT CTTAACAAGC AGTGGGTGGG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42267
    Clone ID Related Accession (Same CDS sequence) XM_009301504.3 , XM_009301504.2
    Accession Version XM_009301504.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC
    ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT
    TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT
    ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC
    AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG
    GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC
    ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC
    CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT
    CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG
    GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG
    CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG
    CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG
    CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG
    AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG
    TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG
    CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC
    TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA
    GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA
    ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC
    CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC
    ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT
    GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG
    AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG
    AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC
    ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT
    CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC
    AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC
    CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC
    TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC
    AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGTGGGTGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299779.1
    CDS1..6585
    Translation

    Target ORF information:

    RefSeq Version XM_009301504.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301504.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC
    ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT
    TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT
    ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC
    AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG
    GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC
    ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC
    CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT
    CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG
    GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG
    CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG
    CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG
    CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG
    AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG
    TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG
    CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC
    TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA
    GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA
    ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC
    CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC
    ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT
    GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG
    AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG
    AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC
    ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT
    CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC
    AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC
    CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC
    TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC
    AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGTGGGTGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42267
    Clone ID Related Accession (Same CDS sequence) XM_009301504.3 , XM_009301504.2
    Accession Version XM_009301504.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC
    ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT
    TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT
    ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC
    AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG
    GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC
    ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC
    CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT
    CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG
    GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG
    CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG
    CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG
    CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG
    AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG
    TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG
    CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC
    TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA
    GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA
    ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC
    CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC
    ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT
    GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG
    AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG
    AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC
    ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT
    CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC
    AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC
    CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC
    TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC
    AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGTGGGTGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299779.1
    CDS639..7223
    Translation

    Target ORF information:

    RefSeq Version XM_009301504.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301504.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT
    ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA
    AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT
    CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG
    TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG
    AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC
    CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT
    GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT
    CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC
    TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG
    CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG
    CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG
    TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG
    ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA
    GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA
    ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT
    GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG
    GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG
    GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT
    GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT
    TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA
    GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT
    CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT
    GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC
    ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG
    CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT
    GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT
    TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT
    CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC
    CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG
    TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG
    GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT
    ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT
    AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA
    AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT
    GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT
    ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT
    CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA
    ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT
    ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC
    AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA
    TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT
    CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA
    TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC
    TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG
    GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT
    TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC
    AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG
    GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT
    CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG
    GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT
    TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA
    ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT
    GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG
    AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC
    TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG
    CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA
    CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG
    AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT
    AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC
    ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT
    TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT
    ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC
    AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG
    GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC
    ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC
    CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT
    CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG
    GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG
    CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG
    CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG
    CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG
    CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG
    AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG
    TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG
    CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC
    TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA
    GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA
    ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC
    CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC
    ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT
    GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG
    AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG
    AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC
    ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT
    CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC
    AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC
    CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC
    TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC
    AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGTGGGTGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42268
    Clone ID Related Accession (Same CDS sequence) XM_009301505.2 , XM_009301505.3
    Accession Version XM_009301505.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG
    ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG
    CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT
    GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT
    TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG
    TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA
    AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA
    GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG
    GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA
    CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG
    GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA
    GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA
    ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG
    GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC
    CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA
    CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC
    CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT
    GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC
    ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG
    CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA
    GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC
    TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG
    CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT
    GCACTGGTGG CATTCGCATT CTCAGGAACC AAGGGCAAAG ACATCAGCAC TATCAAGTCA
    CTGAGGGTGT TGCGAGTCTT GCGACCTCTG AAGACCATCA AACGTCTACC AAAACTGAAG
    GCCGTGTTTG ATTGTGTGGT GAACTCCCTG AAGAATGTCC TGAATATCCT CATTGTCTAC
    ATTCTGTTCA TGTTCATTTT CGCTGTCATT GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT
    TACTGCACAG ACGAGTCCAA GGGCCTGGAG AAAGACTGCA GGGGGCTGTT TTTGGATTAT
    GATAAGGATG ATGTGACGGC ACAACCGAGG GAATGGAAGA AATACGAGTT TCACTATGAC
    AATGTGCTTT GGGCTTTTCT CACTCTCTTC ACTGTTTCTA CGGGAGAGGG CTGGCCTACG
    GTTTTGAAGC ACTCCGTGGA TGCCACTTTT GAGGATCAGG GTCCCAGTCC AGGCTACCGC
    ATTGAGATGT CTATTTTTTA CGTGGTCTAC TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC
    ATTTTTGTAG CCTTGATAAT CATCACATTC CAGGAACAGG GTGATAAGGT CTTGTCAGAG
    TGCAGTCTGG AGAAGAATGA GAGAGCCTGC ATCGACTTTG CTATCAATGC CAAACCTCTG
    ACACGCTACA TGCCGCAGAA CCAGCAGTCC TTTCAGTACC GCCTGTGGAA GTTTGTGGTC
    TCTCCGCCGT TTGAGTACTC CATCATGATC ATGATTGCTC TCAACACTGT GGTGCTCATG
    ATGAAGTTCC ATGGGGCGCC TGACTTTTAT GAGGCAATGC TTAAGTATCT CAACATTGTC
    TTCACTGTTC TCTTTTCACT GGAGTGTATT CTCAAGATTA TTGCTTTCGG CCCCTTGAAT
    TACCTGAAAG ATGCCTGGAA TGTGTTTGAC TTTGTGACTG TGCTGGGCAG CATCACTGAC
    ATTTTAGTCA CTGAGATTAA CGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA
    GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA
    TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC
    ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA
    ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT
    GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT
    CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC
    TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC
    AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT
    ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG
    TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA
    ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC
    TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC
    ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA
    AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA
    GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA
    AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA
    GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC
    GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC
    AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA
    TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC
    TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC
    ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT
    GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA
    GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT
    CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT
    CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT
    ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC
    CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC
    TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT
    GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT
    AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG
    CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC
    CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC
    CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC
    CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG
    CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT
    CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG
    GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG
    TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299780.1
    CDS636..7691
    Translation

    Target ORF information:

    RefSeq Version XM_009301505.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG
    ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG
    CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT
    GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT
    TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG
    TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA
    AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA
    GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG
    GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA
    CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG
    GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA
    GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA
    ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG
    GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC
    CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA
    CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC
    CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT
    GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC
    ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG
    CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA
    GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC
    TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG
    CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT
    GCACTGGTGG CATTCGCATT CTCAGGAACC AAGGGCAAAG ACATCAGCAC TATCAAGTCA
    CTGAGGGTGT TGCGAGTCTT GCGACCTCTG AAGACCATCA AACGTCTACC AAAACTGAAG
    GCCGTGTTTG ATTGTGTGGT GAACTCCCTG AAGAATGTCC TGAATATCCT CATTGTCTAC
    ATTCTGTTCA TGTTCATTTT CGCTGTCATT GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT
    TACTGCACAG ACGAGTCCAA GGGCCTGGAG AAAGACTGCA GGGGGCTGTT TTTGGATTAT
    GATAAGGATG ATGTGACGGC ACAACCGAGG GAATGGAAGA AATACGAGTT TCACTATGAC
    AATGTGCTTT GGGCTTTTCT CACTCTCTTC ACTGTTTCTA CGGGAGAGGG CTGGCCTACG
    GTTTTGAAGC ACTCCGTGGA TGCCACTTTT GAGGATCAGG GTCCCAGTCC AGGCTACCGC
    ATTGAGATGT CTATTTTTTA CGTGGTCTAC TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC
    ATTTTTGTAG CCTTGATAAT CATCACATTC CAGGAACAGG GTGATAAGGT CTTGTCAGAG
    TGCAGTCTGG AGAAGAATGA GAGAGCCTGC ATCGACTTTG CTATCAATGC CAAACCTCTG
    ACACGCTACA TGCCGCAGAA CCAGCAGTCC TTTCAGTACC GCCTGTGGAA GTTTGTGGTC
    TCTCCGCCGT TTGAGTACTC CATCATGATC ATGATTGCTC TCAACACTGT GGTGCTCATG
    ATGAAGTTCC ATGGGGCGCC TGACTTTTAT GAGGCAATGC TTAAGTATCT CAACATTGTC
    TTCACTGTTC TCTTTTCACT GGAGTGTATT CTCAAGATTA TTGCTTTCGG CCCCTTGAAT
    TACCTGAAAG ATGCCTGGAA TGTGTTTGAC TTTGTGACTG TGCTGGGCAG CATCACTGAC
    ATTTTAGTCA CTGAGATTAA CGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA
    GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA
    TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC
    ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA
    ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT
    GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT
    CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC
    TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC
    AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT
    ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG
    TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA
    ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC
    TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC
    ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA
    AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA
    GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA
    AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA
    GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC
    GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC
    AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA
    TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC
    TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC
    ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT
    GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA
    GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT
    CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT
    CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT
    ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC
    CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC
    TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT
    GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT
    AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG
    CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC
    CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC
    CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC
    CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG
    CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT
    CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG
    GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG
    TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42268
    Clone ID Related Accession (Same CDS sequence) XM_009301505.2 , XM_009301505.3
    Accession Version XM_009301505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG
    ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG
    CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT
    GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT
    TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG
    TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA
    AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA
    GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG
    GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA
    CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG
    GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA
    GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA
    ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG
    GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC
    CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA
    CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC
    CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT
    GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC
    ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG
    CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA
    GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC
    TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG
    CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT
    GCACTGGTGG CATTCGCATT CTCAGGAACC AAGGGCAAAG ACATCAGCAC TATCAAGTCA
    CTGAGGGTGT TGCGAGTCTT GCGACCTCTG AAGACCATCA AACGTCTACC AAAACTGAAG
    GCCGTGTTTG ATTGTGTGGT GAACTCCCTG AAGAATGTCC TGAATATCCT CATTGTCTAC
    ATTCTGTTCA TGTTCATTTT CGCTGTCATT GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT
    TACTGCACAG ACGAGTCCAA GGGCCTGGAG AAAGACTGCA GGGGGCTGTT TTTGGATTAT
    GATAAGGATG ATGTGACGGC ACAACCGAGG GAATGGAAGA AATACGAGTT TCACTATGAC
    AATGTGCTTT GGGCTTTTCT CACTCTCTTC ACTGTTTCTA CGGGAGAGGG CTGGCCTACG
    GTTTTGAAGC ACTCCGTGGA TGCCACTTTT GAGGATCAGG GTCCCAGTCC AGGCTACCGC
    ATTGAGATGT CTATTTTTTA CGTGGTCTAC TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC
    ATTTTTGTAG CCTTGATAAT CATCACATTC CAGGAACAGG GTGATAAGGT CTTGTCAGAG
    TGCAGTCTGG AGAAGAATGA GAGAGCCTGC ATCGACTTTG CTATCAATGC CAAACCTCTG
    ACACGCTACA TGCCGCAGAA CCAGCAGTCC TTTCAGTACC GCCTGTGGAA GTTTGTGGTC
    TCTCCGCCGT TTGAGTACTC CATCATGATC ATGATTGCTC TCAACACTGT GGTGCTCATG
    ATGAAGTTCC ATGGGGCGCC TGACTTTTAT GAGGCAATGC TTAAGTATCT CAACATTGTC
    TTCACTGTTC TCTTTTCACT GGAGTGTATT CTCAAGATTA TTGCTTTCGG CCCCTTGAAT
    TACCTGAAAG ATGCCTGGAA TGTGTTTGAC TTTGTGACTG TGCTGGGCAG CATCACTGAC
    ATTTTAGTCA CTGAGATTAA CGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA
    GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA
    TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC
    ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA
    ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT
    GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT
    CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC
    TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC
    AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT
    ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG
    TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA
    ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC
    TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC
    ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA
    AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA
    GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA
    AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA
    GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC
    GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC
    AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA
    TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC
    TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC
    ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT
    GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA
    GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT
    CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT
    CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT
    ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC
    CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC
    TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT
    GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT
    AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG
    CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC
    CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC
    CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC
    CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG
    CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT
    CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG
    GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG
    TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299780.1
    CDS637..7692
    Translation

    Target ORF information:

    RefSeq Version XM_009301505.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
    GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC
    AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA
    CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG
    AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC
    ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC
    ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT
    GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC
    TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC
    TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG
    ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG
    ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC
    ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT
    CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC
    TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC
    GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC
    TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC
    ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG
    GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC
    GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT
    GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG
    CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT
    GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC
    AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC
    CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA
    CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG
    GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC
    GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG
    AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG
    TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG
    AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG
    TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT
    TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG
    TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG
    GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA
    AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA
    GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG
    AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT
    TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA
    CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG
    ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG
    CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT
    GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT
    TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG
    TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA
    AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA
    GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG
    GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA
    CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG
    GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA
    GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA
    ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG
    GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC
    CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA
    CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC
    CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT
    GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC
    ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG
    CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA
    GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC
    TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG
    CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT
    GCACTGGTGG CATTCGCATT CTCAGGAACC AAGGGCAAAG ACATCAGCAC TATCAAGTCA
    CTGAGGGTGT TGCGAGTCTT GCGACCTCTG AAGACCATCA AACGTCTACC AAAACTGAAG
    GCCGTGTTTG ATTGTGTGGT GAACTCCCTG AAGAATGTCC TGAATATCCT CATTGTCTAC
    ATTCTGTTCA TGTTCATTTT CGCTGTCATT GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT
    TACTGCACAG ACGAGTCCAA GGGCCTGGAG AAAGACTGCA GGGGGCTGTT TTTGGATTAT
    GATAAGGATG ATGTGACGGC ACAACCGAGG GAATGGAAGA AATACGAGTT TCACTATGAC
    AATGTGCTTT GGGCTTTTCT CACTCTCTTC ACTGTTTCTA CGGGAGAGGG CTGGCCTACG
    GTTTTGAAGC ACTCCGTGGA TGCCACTTTT GAGGATCAGG GTCCCAGTCC AGGCTACCGC
    ATTGAGATGT CTATTTTTTA CGTGGTCTAC TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC
    ATTTTTGTAG CCTTGATAAT CATCACATTC CAGGAACAGG GTGATAAGGT CTTGTCAGAG
    TGCAGTCTGG AGAAGAATGA GAGAGCCTGC ATCGACTTTG CTATCAATGC CAAACCTCTG
    ACACGCTACA TGCCGCAGAA CCAGCAGTCC TTTCAGTACC GCCTGTGGAA GTTTGTGGTC
    TCTCCGCCGT TTGAGTACTC CATCATGATC ATGATTGCTC TCAACACTGT GGTGCTCATG
    ATGAAGTTCC ATGGGGCGCC TGACTTTTAT GAGGCAATGC TTAAGTATCT CAACATTGTC
    TTCACTGTTC TCTTTTCACT GGAGTGTATT CTCAAGATTA TTGCTTTCGG CCCCTTGAAT
    TACCTGAAAG ATGCCTGGAA TGTGTTTGAC TTTGTGACTG TGCTGGGCAG CATCACTGAC
    ATTTTAGTCA CTGAGATTAA CGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA
    GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA
    TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC
    ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA
    ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT
    GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT
    CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC
    TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC
    AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT
    ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG
    TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA
    ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC
    TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC
    ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA
    AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA
    GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA
    AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA
    GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC
    GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC
    AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA
    TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC
    TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC
    ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT
    GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA
    GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT
    CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT
    CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT
    ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC
    CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC
    TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT
    GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT
    AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG
    CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC
    CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC
    CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC
    CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG
    CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT
    CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG
    GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG
    TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.