prkcz cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prkcz
Entrez Gene ID	555737
Full Name	protein kinase C, zeta
Synonyms	pkcz,si:ch211-150o23.4
General protein information	Preferred Names protein kinase C, zeta Names protein kinase C zeta type atypical protein kinase C zeta
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303846.3	XP_009302121.1	protein kinase C zeta type isoform X1
NM_001030091.1	NP_001025262.1	protein kinase C zeta type
XM_009303846.2	XP_009302121.1	protein kinase C zeta type isoform X1
XM_009303847.2	XP_009302122.1	protein kinase C zeta type isoform X2
XM_009303849.2	XP_009302124.1	protein kinase C zeta type isoform X4
XM_009303848.2	XP_009302123.1	protein kinase C zeta type isoform X3
XM_009303847.3	XP_009302122.1	protein kinase C zeta type isoform X2
XM_009303849.3	XP_009302124.1	protein kinase C zeta type isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKCZ	NP_002735.3
Bos taurus (cattle)	PRKCZ	NP_001071301.1
Gallus gallus (chicken)	PRKCZ	XP_417561.3
Pan troglodytes (chimpanzee)	PRKCZ	XP_001148501.2
Canis lupus familiaris (dog)	PRKCZ	XP_849092.1
Xenopus tropicalis (tropical clawed frog)	prkcz	NP_001093735.1
Mus musculus (house mouse)	Prkcz	NP_032886.2
Rattus norvegicus (Norway rat)	Prkcz	NP_071952.1
Danio rerio (zebrafish)	prkcz	NP_001025262.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Screen for Postembryonic Development in the Zebrafish (
Henke K, Daane JM, Hawkins MB, Dooley CM, Busch-Nentwich EM, Stemple DL, Harris MP
Genetics207(2)609-623(2017 Oct)

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes.
Ribas L, Liew WC, D?az N, Sreenivasan R, Orb?n L, Piferrer F
Proceedings of the National Academy of Sciences of the United States of America114(6)E941-E950(2017 Feb)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta.
Gerlach GF, Wingert RA
Developmental biology396(2)183-200(2014 Dec)

The Par-PrkC polarity complex is required for cilia growth in zebrafish photoreceptors.
Krock BL, Perkins BD
PloS one9(8)e104661(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta.
Title: Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta.

The following prkcz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prkcz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37327	NM_001030091.1 Latest version!	Danio rerio protein kinase C, zeta (prkcz), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa42225	XM_009303846.3 Latest version!	Danio rerio protein kinase C, zeta (prkcz), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa42226	XM_009303847.3 Latest version!	Danio rerio protein kinase C, zeta (prkcz), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa42227	XM_009303848.2 Latest version!	Danio rerio protein kinase C, zeta (prkcz), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa42228	XM_009303849.3 Latest version!	Danio rerio protein kinase C, zeta (prkcz), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa42225	XM_009303846.2	Danio rerio protein kinase C, zeta (prkcz), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa42226	XM_009303847.2	Danio rerio protein kinase C, zeta (prkcz), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa42228	XM_009303849.2	Danio rerio protein kinase C, zeta (prkcz), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prkcz ( NM_001030091.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025262.1 Danio rerio protein kinase C, zeta (prkcz), mRNA.

Price & Availability↑

CloneID	ODa37327
Clone ID Related Accession (Same CDS sequence)	NM_001030091.1
Accession Version	NM_001030091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-27
Organism	Danio rerio(zebrafish)
Product	protein kinase C zeta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ097196.1. On Aug 6, 2005 this sequence version replaced XM_678330.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ097196.1, BC163349.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCTGTCA GTAACGGATC CATGATGGAT TTGCCGTCGG AGTACGTGAA AATTAAAGCA 
CACTACGGAG GGGACATGCT GATCTCAGAC CTGGATTTGG CGCTGACGTA CACAGAGGTG 
TGTAAGGAGG TCAGAGAGAT GTGTGGAGTT CGCAAAGAAA CGCCAATAAC ACTCAAATGG 
ATCGACGACG AAGGAGACCC GTGTACCATC TCATCCCAGA TGGAGCTGGA GGAAGCGTTT 
CGGATCTACA GCCGAAGCCG GCACTCTGGA CTCCTCCTGC ACGTATTTCC AAGTATTCCT 
GAAAAGCCTG GTATGCCGTG CCCAGGAGAG GACAAATCGA TCTACCGCCG TGGAGCTAGA 
CGATGGAGGA AGCTGTACCG GGTGAACGGA CACCTCTTCC AGGCCAAGCG CTTTAACAGG 
AAAGCGTACT GTGGCCACTG CAGTGAGAGG ATATGGGGAC TGGGGCGTCA GGGCTACAAG 
TGTATCAACT GCAAGCTGCT GGTCCATAAA CGCTGTCACA AACTCGTCCC GCTGACCTGT 
CAAAGGCATA TGGATCCAGT CATGCCATCA CAGGAGCCGC TGGTAGACGA TGATAAAAGT 
GGAGAAGAAG TGGAGCTTCC CCCTGAGGAC CCAGAGGACA CAGACGCAAT TCCAGTCCCT 
TTCTTAGCAC ACAACCGAAA GGTGGAGAAA GCTGAAGATG ATTCAGAGGA CCTGAAGGCA 
GTGGTGGATG GTATCGAGGG CATTCAGATC TCTCAGTGTC TCGGTTTGGG GGATTTCGAC 
CTGATTCGAG TCATTGGCAG AGGCAGTTAC GCAAAAGTGC TGCTCGTCCG GCTCAAGAAG 
AACGAGCAGA TTTACGCCAT GAAGGTGGTG AAGAAAGAGC TGGTTCATGA TGATGAGGAC 
ATCGATTGGG TCCAGACAGA AAAACATGTG TTTGAACAAG CATCGACCAA TCCATTTTTA 
GTGGGACTTC ACTCTTGCTT TCAGACAGAG AGCCGGCTCT TTCTAGTGAT CGAGTATGTG 
AATGGAGGAG ATCTCATGTT CCATATGCAG AGGCAGCGGA AGCTTCCAGA GGAACATGCC 
AGATTTTATG CTGCTGAAAT TTGCATCGCT CTTAATTTTC TGCATGAGAA GGGCATTATT 
TACCGGGATT TAAAACTGGA CAATGTACTA TTGGACCAGG ATGGCCATAT CAAACTTACA 
GACTATGGCA TGTGCAAGGA GGGAATCAGA CCAGGTGACA CCACCAGCAC ATTCTGTGGG 
ACGCCCAACT ACATCGCCCC AGAGATCCTG AGGGGAGAGG ATTATGGTTT CAGTGTGGAC 
TGGTGGGCTC TCGGCGTGCT CATGTTTGAG ATGATGGCGG GCCGATCGCC CTTCGATATC 
ATCACTGACA ATCCTGACAT GAACACAGAG GAGTATCTCT TCCAGGTCAT TTTGGAGAAG 
CCGATTCGGA TTCCCAGGTC ATTGTCTGTT AAGGCTGCAA GTGTGCTGAA AGGTTTTCTG 
AACAAGGATC CGAAAGAGAG GCTGGGATGC CAAGTTCAAA CCGGATTCAC AGACATCAAG 
TCTCATACAT TCTTCAGAAG CATAGACTGG GACCAGCTGG AACAGAAGCA GGTGACGCCA 
CCGTTCAAAC CTCAAATCAC AGATGACTAC GGTTTAGAAA ACTTTGACAC ACAGTTCACC 
AACGAACCCG TGCAGCTCAC ACCAGATGAC GAGGATGTGA TAAAGAGAAT AGACCAGTCT 
GAGTTTGAGG GATTCGAGTA CATTAATCCT CTGCTGCTCT CCACTGAAGA AACCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025262.1
CDS	1..1800
Translation	MPVSNGSMMDLPSEYVKIKAHYGGDMLISDLDLALTYTEVCKEVREMCGVRKETPITLKWIDDEGDPCTISSQMELEEAFRIYSRSRHSGLLLHVFPSIPEKPGMPCPGEDKSIYRRGARRWRKLYRVNGHLFQAKRFNRKAYCGHCSERIWGLGRQGYKCINCKLLVHKRCHKLVPLTCQRHMDPVMPSQEPLVDDDKSGEEVELPPEDPEDTDAIPVPFLAHNRKVEKAEDDSEDLKAVVDGIEGIQISQCLGLGDFDLIRVIGRGSYAKVLLVRLKKNEQIYAMKVVKKELVHDDEDIDWVQTEKHVFEQASTNPFLVGLHSCFQTESRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHEKGIIYRDLKLDNVLLDQDGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEEYLFQVILEKPIRIPRSLSVKAASVLKGFLNKDPKERLGCQVQTGFTDIKSHTFFRSIDWDQLEQKQVTPPFKPQITDDYGLENFDTQFTNEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV

Target ORF information:

RefSeq Version	NM_001030091.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, zeta (prkcz), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030091.1

ORF Insert Sequence:

ATGCCTGTCA GTAACGGATC CATGATGGAT TTGCCGTCGG AGTACGTGAA AATTAAAGCA 
CACTACGGAG GGGACATGCT GATCTCAGAC CTGGATTTGG CGCTGACGTA CACAGAGGTG 
TGTAAGGAGG TCAGAGAGAT GTGTGGAGTT CGCAAAGAAA CGCCAATAAC ACTCAAATGG 
ATCGACGACG AAGGAGACCC GTGTACCATC TCATCCCAGA TGGAGCTGGA GGAAGCGTTT 
CGGATCTACA GCCGAAGCCG GCACTCTGGA CTCCTCCTGC ACGTATTTCC AAGTATTCCT 
GAAAAGCCTG GTATGCCGTG CCCAGGAGAG GACAAATCGA TCTACCGCCG TGGAGCTAGA 
CGATGGAGGA AGCTGTACCG GGTGAACGGA CACCTCTTCC AGGCCAAGCG CTTTAACAGG 
AAAGCGTACT GTGGCCACTG CAGTGAGAGG ATATGGGGAC TGGGGCGTCA GGGCTACAAG 
TGTATCAACT GCAAGCTGCT GGTCCATAAA CGCTGTCACA AACTCGTCCC GCTGACCTGT 
CAAAGGCATA TGGATCCAGT CATGCCATCA CAGGAGCCGC TGGTAGACGA TGATAAAAGT 
GGAGAAGAAG TGGAGCTTCC CCCTGAGGAC CCAGAGGACA CAGACGCAAT TCCAGTCCCT 
TTCTTAGCAC ACAACCGAAA GGTGGAGAAA GCTGAAGATG ATTCAGAGGA CCTGAAGGCA 
GTGGTGGATG GTATCGAGGG CATTCAGATC TCTCAGTGTC TCGGTTTGGG GGATTTCGAC 
CTGATTCGAG TCATTGGCAG AGGCAGTTAC GCAAAAGTGC TGCTCGTCCG GCTCAAGAAG 
AACGAGCAGA TTTACGCCAT GAAGGTGGTG AAGAAAGAGC TGGTTCATGA TGATGAGGAC 
ATCGATTGGG TCCAGACAGA AAAACATGTG TTTGAACAAG CATCGACCAA TCCATTTTTA 
GTGGGACTTC ACTCTTGCTT TCAGACAGAG AGCCGGCTCT TTCTAGTGAT CGAGTATGTG 
AATGGAGGAG ATCTCATGTT CCATATGCAG AGGCAGCGGA AGCTTCCAGA GGAACATGCC 
AGATTTTATG CTGCTGAAAT TTGCATCGCT CTTAATTTTC TGCATGAGAA GGGCATTATT 
TACCGGGATT TAAAACTGGA CAATGTACTA TTGGACCAGG ATGGCCATAT CAAACTTACA 
GACTATGGCA TGTGCAAGGA GGGAATCAGA CCAGGTGACA CCACCAGCAC ATTCTGTGGG 
ACGCCCAACT ACATCGCCCC AGAGATCCTG AGGGGAGAGG ATTATGGTTT CAGTGTGGAC 
TGGTGGGCTC TCGGCGTGCT CATGTTTGAG ATGATGGCGG GCCGATCGCC CTTCGATATC 
ATCACTGACA ATCCTGACAT GAACACAGAG GAGTATCTCT TCCAGGTCAT TTTGGAGAAG 
CCGATTCGGA TTCCCAGGTC ATTGTCTGTT AAGGCTGCAA GTGTGCTGAA AGGTTTTCTG 
AACAAGGATC CGAAAGAGAG GCTGGGATGC CAAGTTCAAA CCGGATTCAC AGACATCAAG 
TCTCATACAT TCTTCAGAAG CATAGACTGG GACCAGCTGG AACAGAAGCA GGTGACGCCA 
CCGTTCAAAC CTCAAATCAC AGATGACTAC GGTTTAGAAA ACTTTGACAC ACAGTTCACC 
AACGAACCCG TGCAGCTCAC ACCAGATGAC GAGGATGTGA TAAAGAGAAT AGACCAGTCT 
GAGTTTGAGG GATTCGAGTA CATTAATCCT CTGCTGCTCT CCACTGAAGA AACCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcz ( XM_009303846.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302121.1 Danio rerio protein kinase C, zeta (prkcz), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42225
Clone ID Related Accession (Same CDS sequence)	XM_009303846.3 , XM_009303846.2
Accession Version	XM_009303846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein kinase C zeta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303846.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCA GTAACGGATC CATGATGGAT TTGCCGTCGG AGTACGTGAA AATTAAAGCA 
CACTACGGAG GGGACATGCT GATCTCAGAC CTGGATTTGG CGCTGACGTA CACAGAGGTG 
TGTAAGGAGG TCAGAGAGAT GTGTGGAGTT CGCAAAGAAA CGCCAATAAC ACTCAAATGG 
ATCGACGACG AAGGAGACCC GTGTACCATC TCATCCCAGA TGGAGCTGGA GGAAGCGTTT 
CGGATCTACA GCCGAAACCG GCACTCTGGA CTCCTCCTGC ATGTATTTCC AAGTATTCCT 
GAAAAGCCTG GTATGCCGTG CCCAGGAGAG GACAAATCGA TCTACCGCCG TGGAGCTAGA 
CGATGGAGGA AGCTGTACCG GGTGAACGGA CACCTCTTCC AGGCCAAGCG CTTTAACAGG 
AAAGCGTACT GTGGCCACTG CAGTGAGAGG ATATGGGGAC TGGGGCGTCA GGGCTACAAG 
TGTATCAACT GCAAGCTGCT GGTCCATAAA CGCTGTCACA AACTCGTCCC GCTGACCTGT 
CAAAGGCATA TGGATCCAGT CATGCCATCA CAGGAGCCGC TGGTAGACGA TGATAAAAGT 
GGAGAAGAAG TGGAGCTTCC CCCTGAGGAC CCAGAGGACA CAGACGCAAT TCCAGTCCCT 
TTCTTAGCAC ACAACCGAAA GGTGGAGAAA GCTGAAGATG ATTCAGAGGA CCTGAAGGCA 
GTGGTGGATG GTATCGAGGG CATTCAGATC TCTCAGTGTC TCGGTTTGGG GGATTTCGAC 
CTGATTCGAG TCATTGGCAG AGGCAGTTAC GCAAAAGTGC TGCTCGTCCG GCTCAAGAAG 
AACGAGCAGA TTTACGCCAT GAAGGTGGTG AAGAAAGAGC TGGTTCATGA TGATGAGAGG 
AAGAGAAAGG ACATCGATTG GGTCCAGACA GAAAAACATG TGTTTGAACA AGCATCGACC 
AATCCATTTT TAGTGGGACT TCACTCTTGC TTTCAGACAG AGAGCCGGCT CTTTCTAGTG 
ATCGAGTATG TGAATGGAGG AGATCTCATG TTCCATATGC AGAGGCAGCG GAAGCTTCCA 
GAGGAACATG CCAGATTTTA TGCTGCTGAA ATTTGCATCG CTCTTAATTT TCTGCATGAG 
AAGGGCATTA TTTACCGGGA TTTAAAACTG GACAATGTAC TATTGGACCA GGATGGCCAT 
ATCAAACTTA CAGACTATGG CATGTGCAAG GAGGGAATCA GACCAGGTGA CACCACCAGC 
ACATTCTGTG GGACGCCCAA CTACATCGCC CCAGAGATCC TGAGGGGAGA GGATTATGGT 
TTCAGTGTGG ACTGGTGGGC TCTCGGCGTG CTCATGTTTG AGATGATGGC GGGCCGATCG 
CCCTTCGATA TCATCACTGA CAATCCTGAC ATGAACACAG AGGAGTATCT CTTCCAGGTC 
ATTTTGGAGA AGCCGATTCG GATTCCCAGG TCATTGTCTG TTAAGGCTGC AAGTGTGCTG 
AAAGGTTTTC TGAACAAGGA TCCGAAAGAG AGGCTGGGAT GCCAAGTTCA AACCGGATTC 
ACAGACATCA AGTCTCATAC ATTCTTCAGA AGCATAGACT GGGACCAGCT GGAACAGAAG 
CAGGTGACGC CACCGTTCAA ACCTCAAATC ACAGATGACT ACGGTTTAGA AAACTTTGAC 
ACACAGTTCA CCAACGAACC CGTGCAGCTC ACACCAGATG ATGAGGATGT GATAAAGAGA 
ATAGACCAGT CTGAGTTTGA GGGATTCGAG TACATTAATC CTCTGCTGCT CTCCACTGAA 
GAAACCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302121.1
CDS	931..2742
Translation	MPVSNGSMMDLPSEYVKIKAHYGGDMLISDLDLALTYTEVCKEVREMCGVRKETPITLKWIDDEGDPCTISSQMELEEAFRIYSRNRHSGLLLHVFPSIPEKPGMPCPGEDKSIYRRGARRWRKLYRVNGHLFQAKRFNRKAYCGHCSERIWGLGRQGYKCINCKLLVHKRCHKLVPLTCQRHMDPVMPSQEPLVDDDKSGEEVELPPEDPEDTDAIPVPFLAHNRKVEKAEDDSEDLKAVVDGIEGIQISQCLGLGDFDLIRVIGRGSYAKVLLVRLKKNEQIYAMKVVKKELVHDDERKRKDIDWVQTEKHVFEQASTNPFLVGLHSCFQTESRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHEKGIIYRDLKLDNVLLDQDGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEEYLFQVILEKPIRIPRSLSVKAASVLKGFLNKDPKERLGCQVQTGFTDIKSHTFFRSIDWDQLEQKQVTPPFKPQITDDYGLENFDTQFTNEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV

Target ORF information:

RefSeq Version	XM_009303846.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, zeta (prkcz), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303846.3

ORF Insert Sequence:

ATGCCTGTCA GTAACGGATC CATGATGGAT TTGCCGTCGG AGTACGTGAA AATTAAAGCA 
CACTACGGAG GGGACATGCT GATCTCAGAC CTGGATTTGG CGCTGACGTA CACAGAGGTG 
TGTAAGGAGG TCAGAGAGAT GTGTGGAGTT CGCAAAGAAA CGCCAATAAC ACTCAAATGG 
ATCGACGACG AAGGAGACCC GTGTACCATC TCATCCCAGA TGGAGCTGGA GGAAGCGTTT 
CGGATCTACA GCCGAAACCG GCACTCTGGA CTCCTCCTGC ATGTATTTCC AAGTATTCCT 
GAAAAGCCTG GTATGCCGTG CCCAGGAGAG GACAAATCGA TCTACCGCCG TGGAGCTAGA 
CGATGGAGGA AGCTGTACCG GGTGAACGGA CACCTCTTCC AGGCCAAGCG CTTTAACAGG 
AAAGCGTACT GTGGCCACTG CAGTGAGAGG ATATGGGGAC TGGGGCGTCA GGGCTACAAG 
TGTATCAACT GCAAGCTGCT GGTCCATAAA CGCTGTCACA AACTCGTCCC GCTGACCTGT 
CAAAGGCATA TGGATCCAGT CATGCCATCA CAGGAGCCGC TGGTAGACGA TGATAAAAGT 
GGAGAAGAAG TGGAGCTTCC CCCTGAGGAC CCAGAGGACA CAGACGCAAT TCCAGTCCCT 
TTCTTAGCAC ACAACCGAAA GGTGGAGAAA GCTGAAGATG ATTCAGAGGA CCTGAAGGCA 
GTGGTGGATG GTATCGAGGG CATTCAGATC TCTCAGTGTC TCGGTTTGGG GGATTTCGAC 
CTGATTCGAG TCATTGGCAG AGGCAGTTAC GCAAAAGTGC TGCTCGTCCG GCTCAAGAAG 
AACGAGCAGA TTTACGCCAT GAAGGTGGTG AAGAAAGAGC TGGTTCATGA TGATGAGAGG 
AAGAGAAAGG ACATCGATTG GGTCCAGACA GAAAAACATG TGTTTGAACA AGCATCGACC 
AATCCATTTT TAGTGGGACT TCACTCTTGC TTTCAGACAG AGAGCCGGCT CTTTCTAGTG 
ATCGAGTATG TGAATGGAGG AGATCTCATG TTCCATATGC AGAGGCAGCG GAAGCTTCCA 
GAGGAACATG CCAGATTTTA TGCTGCTGAA ATTTGCATCG CTCTTAATTT TCTGCATGAG 
AAGGGCATTA TTTACCGGGA TTTAAAACTG GACAATGTAC TATTGGACCA GGATGGCCAT 
ATCAAACTTA CAGACTATGG CATGTGCAAG GAGGGAATCA GACCAGGTGA CACCACCAGC 
ACATTCTGTG GGACGCCCAA CTACATCGCC CCAGAGATCC TGAGGGGAGA GGATTATGGT 
TTCAGTGTGG ACTGGTGGGC TCTCGGCGTG CTCATGTTTG AGATGATGGC GGGCCGATCG 
CCCTTCGATA TCATCACTGA CAATCCTGAC ATGAACACAG AGGAGTATCT CTTCCAGGTC 
ATTTTGGAGA AGCCGATTCG GATTCCCAGG TCATTGTCTG TTAAGGCTGC AAGTGTGCTG 
AAAGGTTTTC TGAACAAGGA TCCGAAAGAG AGGCTGGGAT GCCAAGTTCA AACCGGATTC 
ACAGACATCA AGTCTCATAC ATTCTTCAGA AGCATAGACT GGGACCAGCT GGAACAGAAG 
CAGGTGACGC CACCGTTCAA ACCTCAAATC ACAGATGACT ACGGTTTAGA AAACTTTGAC 
ACACAGTTCA CCAACGAACC CGTGCAGCTC ACACCAGATG ATGAGGATGT GATAAAGAGA 
ATAGACCAGT CTGAGTTTGA GGGATTCGAG TACATTAATC CTCTGCTGCT CTCCACTGAA 
GAAACCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcz ( XM_009303846.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302121.1 Danio rerio protein kinase C, zeta (prkcz), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42225
Clone ID Related Accession (Same CDS sequence)	XM_009303846.3 , XM_009303846.2
Accession Version	XM_009303846.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein kinase C zeta type isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCA GTAACGGATC CATGATGGAT TTGCCGTCGG AGTACGTGAA AATTAAAGCA 
CACTACGGAG GGGACATGCT GATCTCAGAC CTGGATTTGG CGCTGACGTA CACAGAGGTG 
TGTAAGGAGG TCAGAGAGAT GTGTGGAGTT CGCAAAGAAA CGCCAATAAC ACTCAAATGG 
ATCGACGACG AAGGAGACCC GTGTACCATC TCATCCCAGA TGGAGCTGGA GGAAGCGTTT 
CGGATCTACA GCCGAAACCG GCACTCTGGA CTCCTCCTGC ATGTATTTCC AAGTATTCCT 
GAAAAGCCTG GTATGCCGTG CCCAGGAGAG GACAAATCGA TCTACCGCCG TGGAGCTAGA 
CGATGGAGGA AGCTGTACCG GGTGAACGGA CACCTCTTCC AGGCCAAGCG CTTTAACAGG 
AAAGCGTACT GTGGCCACTG CAGTGAGAGG ATATGGGGAC TGGGGCGTCA GGGCTACAAG 
TGTATCAACT GCAAGCTGCT GGTCCATAAA CGCTGTCACA AACTCGTCCC GCTGACCTGT 
CAAAGGCATA TGGATCCAGT CATGCCATCA CAGGAGCCGC TGGTAGACGA TGATAAAAGT 
GGAGAAGAAG TGGAGCTTCC CCCTGAGGAC CCAGAGGACA CAGACGCAAT TCCAGTCCCT 
TTCTTAGCAC ACAACCGAAA GGTGGAGAAA GCTGAAGATG ATTCAGAGGA CCTGAAGGCA 
GTGGTGGATG GTATCGAGGG CATTCAGATC TCTCAGTGTC TCGGTTTGGG GGATTTCGAC 
CTGATTCGAG TCATTGGCAG AGGCAGTTAC GCAAAAGTGC TGCTCGTCCG GCTCAAGAAG 
AACGAGCAGA TTTACGCCAT GAAGGTGGTG AAGAAAGAGC TGGTTCATGA TGATGAGAGG 
AAGAGAAAGG ACATCGATTG GGTCCAGACA GAAAAACATG TGTTTGAACA AGCATCGACC 
AATCCATTTT TAGTGGGACT TCACTCTTGC TTTCAGACAG AGAGCCGGCT CTTTCTAGTG 
ATCGAGTATG TGAATGGAGG AGATCTCATG TTCCATATGC AGAGGCAGCG GAAGCTTCCA 
GAGGAACATG CCAGATTTTA TGCTGCTGAA ATTTGCATCG CTCTTAATTT TCTGCATGAG 
AAGGGCATTA TTTACCGGGA TTTAAAACTG GACAATGTAC TATTGGACCA GGATGGCCAT 
ATCAAACTTA CAGACTATGG CATGTGCAAG GAGGGAATCA GACCAGGTGA CACCACCAGC 
ACATTCTGTG GGACGCCCAA CTACATCGCC CCAGAGATCC TGAGGGGAGA GGATTATGGT 
TTCAGTGTGG ACTGGTGGGC TCTCGGCGTG CTCATGTTTG AGATGATGGC GGGCCGATCG 
CCCTTCGATA TCATCACTGA CAATCCTGAC ATGAACACAG AGGAGTATCT CTTCCAGGTC 
ATTTTGGAGA AGCCGATTCG GATTCCCAGG TCATTGTCTG TTAAGGCTGC AAGTGTGCTG 
AAAGGTTTTC TGAACAAGGA TCCGAAAGAG AGGCTGGGAT GCCAAGTTCA AACCGGATTC 
ACAGACATCA AGTCTCATAC ATTCTTCAGA AGCATAGACT GGGACCAGCT GGAACAGAAG 
CAGGTGACGC CACCGTTCAA ACCTCAAATC ACAGATGACT ACGGTTTAGA AAACTTTGAC 
ACACAGTTCA CCAACGAACC CGTGCAGCTC ACACCAGATG ATGAGGATGT GATAAAGAGA 
ATAGACCAGT CTGAGTTTGA GGGATTCGAG TACATTAATC CTCTGCTGCT CTCCACTGAA 
GAAACCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302121.1
CDS	963..2774
Translation	MPVSNGSMMDLPSEYVKIKAHYGGDMLISDLDLALTYTEVCKEVREMCGVRKETPITLKWIDDEGDPCTISSQMELEEAFRIYSRNRHSGLLLHVFPSIPEKPGMPCPGEDKSIYRRGARRWRKLYRVNGHLFQAKRFNRKAYCGHCSERIWGLGRQGYKCINCKLLVHKRCHKLVPLTCQRHMDPVMPSQEPLVDDDKSGEEVELPPEDPEDTDAIPVPFLAHNRKVEKAEDDSEDLKAVVDGIEGIQISQCLGLGDFDLIRVIGRGSYAKVLLVRLKKNEQIYAMKVVKKELVHDDERKRKDIDWVQTEKHVFEQASTNPFLVGLHSCFQTESRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHEKGIIYRDLKLDNVLLDQDGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEEYLFQVILEKPIRIPRSLSVKAASVLKGFLNKDPKERLGCQVQTGFTDIKSHTFFRSIDWDQLEQKQVTPPFKPQITDDYGLENFDTQFTNEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV

Target ORF information:

RefSeq Version	XM_009303846.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase C, zeta (prkcz), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303846.2

ORF Insert Sequence:

ATGCCTGTCA GTAACGGATC CATGATGGAT TTGCCGTCGG AGTACGTGAA AATTAAAGCA 
CACTACGGAG GGGACATGCT GATCTCAGAC CTGGATTTGG CGCTGACGTA CACAGAGGTG 
TGTAAGGAGG TCAGAGAGAT GTGTGGAGTT CGCAAAGAAA CGCCAATAAC ACTCAAATGG 
ATCGACGACG AAGGAGACCC GTGTACCATC TCATCCCAGA TGGAGCTGGA GGAAGCGTTT 
CGGATCTACA GCCGAAACCG GCACTCTGGA CTCCTCCTGC ATGTATTTCC AAGTATTCCT 
GAAAAGCCTG GTATGCCGTG CCCAGGAGAG GACAAATCGA TCTACCGCCG TGGAGCTAGA 
CGATGGAGGA AGCTGTACCG GGTGAACGGA CACCTCTTCC AGGCCAAGCG CTTTAACAGG 
AAAGCGTACT GTGGCCACTG CAGTGAGAGG ATATGGGGAC TGGGGCGTCA GGGCTACAAG 
TGTATCAACT GCAAGCTGCT GGTCCATAAA CGCTGTCACA AACTCGTCCC GCTGACCTGT 
CAAAGGCATA TGGATCCAGT CATGCCATCA CAGGAGCCGC TGGTAGACGA TGATAAAAGT 
GGAGAAGAAG TGGAGCTTCC CCCTGAGGAC CCAGAGGACA CAGACGCAAT TCCAGTCCCT 
TTCTTAGCAC ACAACCGAAA GGTGGAGAAA GCTGAAGATG ATTCAGAGGA CCTGAAGGCA 
GTGGTGGATG GTATCGAGGG CATTCAGATC TCTCAGTGTC TCGGTTTGGG GGATTTCGAC 
CTGATTCGAG TCATTGGCAG AGGCAGTTAC GCAAAAGTGC TGCTCGTCCG GCTCAAGAAG 
AACGAGCAGA TTTACGCCAT GAAGGTGGTG AAGAAAGAGC TGGTTCATGA TGATGAGAGG 
AAGAGAAAGG ACATCGATTG GGTCCAGACA GAAAAACATG TGTTTGAACA AGCATCGACC 
AATCCATTTT TAGTGGGACT TCACTCTTGC TTTCAGACAG AGAGCCGGCT CTTTCTAGTG 
ATCGAGTATG TGAATGGAGG AGATCTCATG TTCCATATGC AGAGGCAGCG GAAGCTTCCA 
GAGGAACATG CCAGATTTTA TGCTGCTGAA ATTTGCATCG CTCTTAATTT TCTGCATGAG 
AAGGGCATTA TTTACCGGGA TTTAAAACTG GACAATGTAC TATTGGACCA GGATGGCCAT 
ATCAAACTTA CAGACTATGG CATGTGCAAG GAGGGAATCA GACCAGGTGA CACCACCAGC 
ACATTCTGTG GGACGCCCAA CTACATCGCC CCAGAGATCC TGAGGGGAGA GGATTATGGT 
TTCAGTGTGG ACTGGTGGGC TCTCGGCGTG CTCATGTTTG AGATGATGGC GGGCCGATCG 
CCCTTCGATA TCATCACTGA CAATCCTGAC ATGAACACAG AGGAGTATCT CTTCCAGGTC 
ATTTTGGAGA AGCCGATTCG GATTCCCAGG TCATTGTCTG TTAAGGCTGC AAGTGTGCTG 
AAAGGTTTTC TGAACAAGGA TCCGAAAGAG AGGCTGGGAT GCCAAGTTCA AACCGGATTC 
ACAGACATCA AGTCTCATAC ATTCTTCAGA AGCATAGACT GGGACCAGCT GGAACAGAAG 
CAGGTGACGC CACCGTTCAA ACCTCAAATC ACAGATGACT ACGGTTTAGA AAACTTTGAC 
ACACAGTTCA CCAACGAACC CGTGCAGCTC ACACCAGATG ATGAGGATGT GATAAAGAGA 
ATAGACCAGT CTGAGTTTGA GGGATTCGAG TACATTAATC CTCTGCTGCT CTCCACTGAA 
GAAACCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcz ( XM_009303847.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302122.1 Danio rerio protein kinase C, zeta (prkcz), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42226
Clone ID Related Accession (Same CDS sequence)	XM_009303847.2 , XM_009303847.3
Accession Version	XM_009303847.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein kinase C zeta type isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTAA CACTGCTGTT CTATCTGCGA GTGAAAGAAT CGATCTACCG CCGTGGAGCT 
AGACGATGGA GGAAGCTGTA CCGGGTGAAC GGACACCTCT TCCAGGCCAA GCGCTTTAAC 
AGGAAAGCGT ACTGTGGCCA CTGCAGTGAG AGGATATGGG GACTGGGGCG TCAGGGCTAC 
AAGTGTATCA ACTGCAAGCT GCTGGTCCAT AAACGCTGTC ACAAACTCGT CCCGCTGACC 
TGTCAAAGGC ATATGGATCC AGTCATGCCA TCACAGGAGC CGCTGGTAGA CGATGATAAA 
AGTGGAGAAG AAGTGGAGCT TCCCCCTGAG GACCCAGAGG ACACAGACGC AATTCCAGTC 
CCTTTCTTAG CACACAACCG AAAGGTGGAG AAAGCTGAAG ATGATTCAGA GGACCTGAAG 
GCAGTGGTGG ATGGTATCGA GGGCATTCAG ATCTCTCAGT GTCTCGGTTT GGGGGATTTC 
GACCTGATTC GAGTCATTGG CAGAGGCAGT TACGCAAAAG TGCTGCTCGT CCGGCTCAAG 
AAGAACGAGC AGATTTACGC CATGAAGGTG GTGAAGAAAG AGCTGGTTCA TGATGATGAG 
AGGAAGAGAA AGGACATCGA TTGGGTCCAG ACAGAAAAAC ATGTGTTTGA ACAAGCATCG 
ACCAATCCAT TTTTAGTGGG ACTTCACTCT TGCTTTCAGA CAGAGAGCCG GCTCTTTCTA 
GTGATCGAGT ATGTGAATGG AGGAGATCTC ATGTTCCATA TGCAGAGGCA GCGGAAGCTT 
CCAGAGGAAC ATGCCAGATT TTATGCTGCT GAAATTTGCA TCGCTCTTAA TTTTCTGCAT 
GAGAAGGGCA TTATTTACCG GGATTTAAAA CTGGACAATG TACTATTGGA CCAGGATGGC 
CATATCAAAC TTACAGACTA TGGCATGTGC AAGGAGGGAA TCAGACCAGG TGACACCACC 
AGCACATTCT GTGGGACGCC CAACTACATC GCCCCAGAGA TCCTGAGGGG AGAGGATTAT 
GGTTTCAGTG TGGACTGGTG GGCTCTCGGC GTGCTCATGT TTGAGATGAT GGCGGGCCGA 
TCGCCCTTCG ATATCATCAC TGACAATCCT GACATGAACA CAGAGGAGTA TCTCTTCCAG 
GTCATTTTGG AGAAGCCGAT TCGGATTCCC AGGTCATTGT CTGTTAAGGC TGCAAGTGTG 
CTGAAAGGTT TTCTGAACAA GGATCCGAAA GAGAGGCTGG GATGCCAAGT TCAAACCGGA 
TTCACAGACA TCAAGTCTCA TACATTCTTC AGAAGCATAG ACTGGGACCA GCTGGAACAG 
AAGCAGGTGA CGCCACCGTT CAAACCTCAA ATCACAGATG ACTACGGTTT AGAAAACTTT 
GACACACAGT TCACCAACGA ACCCGTGCAG CTCACACCAG ATGATGAGGA TGTGATAAAG 
AGAATAGACC AGTCTGAGTT TGAGGGATTC GAGTACATTA ATCCTCTGCT GCTCTCCACT 
GAAGAAACCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302122.1
CDS	185..1699
Translation	MLLTLLFYLRVKESIYRRGARRWRKLYRVNGHLFQAKRFNRKAYCGHCSERIWGLGRQGYKCINCKLLVHKRCHKLVPLTCQRHMDPVMPSQEPLVDDDKSGEEVELPPEDPEDTDAIPVPFLAHNRKVEKAEDDSEDLKAVVDGIEGIQISQCLGLGDFDLIRVIGRGSYAKVLLVRLKKNEQIYAMKVVKKELVHDDERKRKDIDWVQTEKHVFEQASTNPFLVGLHSCFQTESRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHEKGIIYRDLKLDNVLLDQDGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEEYLFQVILEKPIRIPRSLSVKAASVLKGFLNKDPKERLGCQVQTGFTDIKSHTFFRSIDWDQLEQKQVTPPFKPQITDDYGLENFDTQFTNEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV

Target ORF information:

RefSeq Version	XM_009303847.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase C, zeta (prkcz), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303847.2

ORF Insert Sequence:

ATGCTGCTAA CACTGCTGTT CTATCTGCGA GTGAAAGAAT CGATCTACCG CCGTGGAGCT 
AGACGATGGA GGAAGCTGTA CCGGGTGAAC GGACACCTCT TCCAGGCCAA GCGCTTTAAC 
AGGAAAGCGT ACTGTGGCCA CTGCAGTGAG AGGATATGGG GACTGGGGCG TCAGGGCTAC 
AAGTGTATCA ACTGCAAGCT GCTGGTCCAT AAACGCTGTC ACAAACTCGT CCCGCTGACC 
TGTCAAAGGC ATATGGATCC AGTCATGCCA TCACAGGAGC CGCTGGTAGA CGATGATAAA 
AGTGGAGAAG AAGTGGAGCT TCCCCCTGAG GACCCAGAGG ACACAGACGC AATTCCAGTC 
CCTTTCTTAG CACACAACCG AAAGGTGGAG AAAGCTGAAG ATGATTCAGA GGACCTGAAG 
GCAGTGGTGG ATGGTATCGA GGGCATTCAG ATCTCTCAGT GTCTCGGTTT GGGGGATTTC 
GACCTGATTC GAGTCATTGG CAGAGGCAGT TACGCAAAAG TGCTGCTCGT CCGGCTCAAG 
AAGAACGAGC AGATTTACGC CATGAAGGTG GTGAAGAAAG AGCTGGTTCA TGATGATGAG 
AGGAAGAGAA AGGACATCGA TTGGGTCCAG ACAGAAAAAC ATGTGTTTGA ACAAGCATCG 
ACCAATCCAT TTTTAGTGGG ACTTCACTCT TGCTTTCAGA CAGAGAGCCG GCTCTTTCTA 
GTGATCGAGT ATGTGAATGG AGGAGATCTC ATGTTCCATA TGCAGAGGCA GCGGAAGCTT 
CCAGAGGAAC ATGCCAGATT TTATGCTGCT GAAATTTGCA TCGCTCTTAA TTTTCTGCAT 
GAGAAGGGCA TTATTTACCG GGATTTAAAA CTGGACAATG TACTATTGGA CCAGGATGGC 
CATATCAAAC TTACAGACTA TGGCATGTGC AAGGAGGGAA TCAGACCAGG TGACACCACC 
AGCACATTCT GTGGGACGCC CAACTACATC GCCCCAGAGA TCCTGAGGGG AGAGGATTAT 
GGTTTCAGTG TGGACTGGTG GGCTCTCGGC GTGCTCATGT TTGAGATGAT GGCGGGCCGA 
TCGCCCTTCG ATATCATCAC TGACAATCCT GACATGAACA CAGAGGAGTA TCTCTTCCAG 
GTCATTTTGG AGAAGCCGAT TCGGATTCCC AGGTCATTGT CTGTTAAGGC TGCAAGTGTG 
CTGAAAGGTT TTCTGAACAA GGATCCGAAA GAGAGGCTGG GATGCCAAGT TCAAACCGGA 
TTCACAGACA TCAAGTCTCA TACATTCTTC AGAAGCATAG ACTGGGACCA GCTGGAACAG 
AAGCAGGTGA CGCCACCGTT CAAACCTCAA ATCACAGATG ACTACGGTTT AGAAAACTTT 
GACACACAGT TCACCAACGA ACCCGTGCAG CTCACACCAG ATGATGAGGA TGTGATAAAG 
AGAATAGACC AGTCTGAGTT TGAGGGATTC GAGTACATTA ATCCTCTGCT GCTCTCCACT 
GAAGAAACCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcz ( XM_009303847.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302122.1 Danio rerio protein kinase C, zeta (prkcz), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42226
Clone ID Related Accession (Same CDS sequence)	XM_009303847.2 , XM_009303847.3
Accession Version	XM_009303847.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein kinase C zeta type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303847.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTAA CACTGCTGTT CTATCTGCGA GTGAAAGAAT CGATCTACCG CCGTGGAGCT 
AGACGATGGA GGAAGCTGTA CCGGGTGAAC GGACACCTCT TCCAGGCCAA GCGCTTTAAC 
AGGAAAGCGT ACTGTGGCCA CTGCAGTGAG AGGATATGGG GACTGGGGCG TCAGGGCTAC 
AAGTGTATCA ACTGCAAGCT GCTGGTCCAT AAACGCTGTC ACAAACTCGT CCCGCTGACC 
TGTCAAAGGC ATATGGATCC AGTCATGCCA TCACAGGAGC CGCTGGTAGA CGATGATAAA 
AGTGGAGAAG AAGTGGAGCT TCCCCCTGAG GACCCAGAGG ACACAGACGC AATTCCAGTC 
CCTTTCTTAG CACACAACCG AAAGGTGGAG AAAGCTGAAG ATGATTCAGA GGACCTGAAG 
GCAGTGGTGG ATGGTATCGA GGGCATTCAG ATCTCTCAGT GTCTCGGTTT GGGGGATTTC 
GACCTGATTC GAGTCATTGG CAGAGGCAGT TACGCAAAAG TGCTGCTCGT CCGGCTCAAG 
AAGAACGAGC AGATTTACGC CATGAAGGTG GTGAAGAAAG AGCTGGTTCA TGATGATGAG 
AGGAAGAGAA AGGACATCGA TTGGGTCCAG ACAGAAAAAC ATGTGTTTGA ACAAGCATCG 
ACCAATCCAT TTTTAGTGGG ACTTCACTCT TGCTTTCAGA CAGAGAGCCG GCTCTTTCTA 
GTGATCGAGT ATGTGAATGG AGGAGATCTC ATGTTCCATA TGCAGAGGCA GCGGAAGCTT 
CCAGAGGAAC ATGCCAGATT TTATGCTGCT GAAATTTGCA TCGCTCTTAA TTTTCTGCAT 
GAGAAGGGCA TTATTTACCG GGATTTAAAA CTGGACAATG TACTATTGGA CCAGGATGGC 
CATATCAAAC TTACAGACTA TGGCATGTGC AAGGAGGGAA TCAGACCAGG TGACACCACC 
AGCACATTCT GTGGGACGCC CAACTACATC GCCCCAGAGA TCCTGAGGGG AGAGGATTAT 
GGTTTCAGTG TGGACTGGTG GGCTCTCGGC GTGCTCATGT TTGAGATGAT GGCGGGCCGA 
TCGCCCTTCG ATATCATCAC TGACAATCCT GACATGAACA CAGAGGAGTA TCTCTTCCAG 
GTCATTTTGG AGAAGCCGAT TCGGATTCCC AGGTCATTGT CTGTTAAGGC TGCAAGTGTG 
CTGAAAGGTT TTCTGAACAA GGATCCGAAA GAGAGGCTGG GATGCCAAGT TCAAACCGGA 
TTCACAGACA TCAAGTCTCA TACATTCTTC AGAAGCATAG ACTGGGACCA GCTGGAACAG 
AAGCAGGTGA CGCCACCGTT CAAACCTCAA ATCACAGATG ACTACGGTTT AGAAAACTTT 
GACACACAGT TCACCAACGA ACCCGTGCAG CTCACACCAG ATGATGAGGA TGTGATAAAG 
AGAATAGACC AGTCTGAGTT TGAGGGATTC GAGTACATTA ATCCTCTGCT GCTCTCCACT 
GAAGAAACCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302122.1
CDS	169..1683
Translation	MLLTLLFYLRVKESIYRRGARRWRKLYRVNGHLFQAKRFNRKAYCGHCSERIWGLGRQGYKCINCKLLVHKRCHKLVPLTCQRHMDPVMPSQEPLVDDDKSGEEVELPPEDPEDTDAIPVPFLAHNRKVEKAEDDSEDLKAVVDGIEGIQISQCLGLGDFDLIRVIGRGSYAKVLLVRLKKNEQIYAMKVVKKELVHDDERKRKDIDWVQTEKHVFEQASTNPFLVGLHSCFQTESRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHEKGIIYRDLKLDNVLLDQDGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEEYLFQVILEKPIRIPRSLSVKAASVLKGFLNKDPKERLGCQVQTGFTDIKSHTFFRSIDWDQLEQKQVTPPFKPQITDDYGLENFDTQFTNEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV

Target ORF information:

RefSeq Version	XM_009303847.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, zeta (prkcz), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303847.3

ORF Insert Sequence:

ATGCTGCTAA CACTGCTGTT CTATCTGCGA GTGAAAGAAT CGATCTACCG CCGTGGAGCT 
AGACGATGGA GGAAGCTGTA CCGGGTGAAC GGACACCTCT TCCAGGCCAA GCGCTTTAAC 
AGGAAAGCGT ACTGTGGCCA CTGCAGTGAG AGGATATGGG GACTGGGGCG TCAGGGCTAC 
AAGTGTATCA ACTGCAAGCT GCTGGTCCAT AAACGCTGTC ACAAACTCGT CCCGCTGACC 
TGTCAAAGGC ATATGGATCC AGTCATGCCA TCACAGGAGC CGCTGGTAGA CGATGATAAA 
AGTGGAGAAG AAGTGGAGCT TCCCCCTGAG GACCCAGAGG ACACAGACGC AATTCCAGTC 
CCTTTCTTAG CACACAACCG AAAGGTGGAG AAAGCTGAAG ATGATTCAGA GGACCTGAAG 
GCAGTGGTGG ATGGTATCGA GGGCATTCAG ATCTCTCAGT GTCTCGGTTT GGGGGATTTC 
GACCTGATTC GAGTCATTGG CAGAGGCAGT TACGCAAAAG TGCTGCTCGT CCGGCTCAAG 
AAGAACGAGC AGATTTACGC CATGAAGGTG GTGAAGAAAG AGCTGGTTCA TGATGATGAG 
AGGAAGAGAA AGGACATCGA TTGGGTCCAG ACAGAAAAAC ATGTGTTTGA ACAAGCATCG 
ACCAATCCAT TTTTAGTGGG ACTTCACTCT TGCTTTCAGA CAGAGAGCCG GCTCTTTCTA 
GTGATCGAGT ATGTGAATGG AGGAGATCTC ATGTTCCATA TGCAGAGGCA GCGGAAGCTT 
CCAGAGGAAC ATGCCAGATT TTATGCTGCT GAAATTTGCA TCGCTCTTAA TTTTCTGCAT 
GAGAAGGGCA TTATTTACCG GGATTTAAAA CTGGACAATG TACTATTGGA CCAGGATGGC 
CATATCAAAC TTACAGACTA TGGCATGTGC AAGGAGGGAA TCAGACCAGG TGACACCACC 
AGCACATTCT GTGGGACGCC CAACTACATC GCCCCAGAGA TCCTGAGGGG AGAGGATTAT 
GGTTTCAGTG TGGACTGGTG GGCTCTCGGC GTGCTCATGT TTGAGATGAT GGCGGGCCGA 
TCGCCCTTCG ATATCATCAC TGACAATCCT GACATGAACA CAGAGGAGTA TCTCTTCCAG 
GTCATTTTGG AGAAGCCGAT TCGGATTCCC AGGTCATTGT CTGTTAAGGC TGCAAGTGTG 
CTGAAAGGTT TTCTGAACAA GGATCCGAAA GAGAGGCTGG GATGCCAAGT TCAAACCGGA 
TTCACAGACA TCAAGTCTCA TACATTCTTC AGAAGCATAG ACTGGGACCA GCTGGAACAG 
AAGCAGGTGA CGCCACCGTT CAAACCTCAA ATCACAGATG ACTACGGTTT AGAAAACTTT 
GACACACAGT TCACCAACGA ACCCGTGCAG CTCACACCAG ATGATGAGGA TGTGATAAAG 
AGAATAGACC AGTCTGAGTT TGAGGGATTC GAGTACATTA ATCCTCTGCT GCTCTCCACT 
GAAGAAACCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcz ( XM_009303848.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302123.1 Danio rerio protein kinase C, zeta (prkcz), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42227
Clone ID Related Accession (Same CDS sequence)	XM_009303848.2
Accession Version	XM_009303848.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein kinase C zeta type isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCCTA AGAAGGCTGA CGAATCGATC TACCGCCGTG GAGCTAGACG ATGGAGGAAG 
CTGTACCGGG TGAACGGACA CCTCTTCCAG GCCAAGCGCT TTAACAGGAA AGCGTACTGT 
GGCCACTGCA GTGAGAGGAT ATGGGGACTG GGGCGTCAGG GCTACAAGTG TATCAACTGC 
AAGCTGCTGG TCCATAAACG CTGTCACAAA CTCGTCCCGC TGACCTGTCA AAGGCATATG 
GATCCAGTCA TGCCATCACA GGAGCCGCTG GTAGACGATG ATAAAAGTGG AGAAGAAGTG 
GAGCTTCCCC CTGAGGACCC AGAGGACACA GACGCAATTC CAGTCCCTTT CTTAGCACAC 
AACCGAAAGG TGGAGAAAGC TGAAGATGAT TCAGAGGACC TGAAGGCAGT GGTGGATGGT 
ATCGAGGGCA TTCAGATCTC TCAGTGTCTC GGTTTGGGGG ATTTCGACCT GATTCGAGTC 
ATTGGCAGAG GCAGTTACGC AAAAGTGCTG CTCGTCCGGC TCAAGAAGAA CGAGCAGATT 
TACGCCATGA AGGTGGTGAA GAAAGAGCTG GTTCATGATG ATGAGAGGAA GAGAAAGGAC 
ATCGATTGGG TCCAGACAGA AAAACATGTG TTTGAACAAG CATCGACCAA TCCATTTTTA 
GTGGGACTTC ACTCTTGCTT TCAGACAGAG AGCCGGCTCT TTCTAGTGAT CGAGTATGTG 
AATGGAGGAG ATCTCATGTT CCATATGCAG AGGCAGCGGA AGCTTCCAGA GGAACATGCC 
AGATTTTATG CTGCTGAAAT TTGCATCGCT CTTAATTTTC TGCATGAGAA GGGCATTATT 
TACCGGGATT TAAAACTGGA CAATGTACTA TTGGACCAGG ATGGCCATAT CAAACTTACA 
GACTATGGCA TGTGCAAGGA GGGAATCAGA CCAGGTGACA CCACCAGCAC ATTCTGTGGG 
ACGCCCAACT ACATCGCCCC AGAGATCCTG AGGGGAGAGG ATTATGGTTT CAGTGTGGAC 
TGGTGGGCTC TCGGCGTGCT CATGTTTGAG ATGATGGCGG GCCGATCGCC CTTCGATATC 
ATCACTGACA ATCCTGACAT GAACACAGAG GAGTATCTCT TCCAGGTCAT TTTGGAGAAG 
CCGATTCGGA TTCCCAGGTC ATTGTCTGTT AAGGCTGCAA GTGTGCTGAA AGGTTTTCTG 
AACAAGGATC CGAAAGAGAG GCTGGGATGC CAAGTTCAAA CCGGATTCAC AGACATCAAG 
TCTCATACAT TCTTCAGAAG CATAGACTGG GACCAGCTGG AACAGAAGCA GGTGACGCCA 
CCGTTCAAAC CTCAAATCAC AGATGACTAC GGTTTAGAAA ACTTTGACAC ACAGTTCACC 
AACGAACCCG TGCAGCTCAC ACCAGATGAT GAGGATGTGA TAAAGAGAAT AGACCAGTCT 
GAGTTTGAGG GATTCGAGTA CATTAATCCT CTGCTGCTCT CCACTGAAGA AACCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302123.1
CDS	97..1596
Translation	MHPKKADESIYRRGARRWRKLYRVNGHLFQAKRFNRKAYCGHCSERIWGLGRQGYKCINCKLLVHKRCHKLVPLTCQRHMDPVMPSQEPLVDDDKSGEEVELPPEDPEDTDAIPVPFLAHNRKVEKAEDDSEDLKAVVDGIEGIQISQCLGLGDFDLIRVIGRGSYAKVLLVRLKKNEQIYAMKVVKKELVHDDERKRKDIDWVQTEKHVFEQASTNPFLVGLHSCFQTESRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHEKGIIYRDLKLDNVLLDQDGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEEYLFQVILEKPIRIPRSLSVKAASVLKGFLNKDPKERLGCQVQTGFTDIKSHTFFRSIDWDQLEQKQVTPPFKPQITDDYGLENFDTQFTNEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV

Target ORF information:

RefSeq Version	XM_009303848.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase C, zeta (prkcz), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303848.2

ORF Insert Sequence:

ATGCATCCTA AGAAGGCTGA CGAATCGATC TACCGCCGTG GAGCTAGACG ATGGAGGAAG 
CTGTACCGGG TGAACGGACA CCTCTTCCAG GCCAAGCGCT TTAACAGGAA AGCGTACTGT 
GGCCACTGCA GTGAGAGGAT ATGGGGACTG GGGCGTCAGG GCTACAAGTG TATCAACTGC 
AAGCTGCTGG TCCATAAACG CTGTCACAAA CTCGTCCCGC TGACCTGTCA AAGGCATATG 
GATCCAGTCA TGCCATCACA GGAGCCGCTG GTAGACGATG ATAAAAGTGG AGAAGAAGTG 
GAGCTTCCCC CTGAGGACCC AGAGGACACA GACGCAATTC CAGTCCCTTT CTTAGCACAC 
AACCGAAAGG TGGAGAAAGC TGAAGATGAT TCAGAGGACC TGAAGGCAGT GGTGGATGGT 
ATCGAGGGCA TTCAGATCTC TCAGTGTCTC GGTTTGGGGG ATTTCGACCT GATTCGAGTC 
ATTGGCAGAG GCAGTTACGC AAAAGTGCTG CTCGTCCGGC TCAAGAAGAA CGAGCAGATT 
TACGCCATGA AGGTGGTGAA GAAAGAGCTG GTTCATGATG ATGAGAGGAA GAGAAAGGAC 
ATCGATTGGG TCCAGACAGA AAAACATGTG TTTGAACAAG CATCGACCAA TCCATTTTTA 
GTGGGACTTC ACTCTTGCTT TCAGACAGAG AGCCGGCTCT TTCTAGTGAT CGAGTATGTG 
AATGGAGGAG ATCTCATGTT CCATATGCAG AGGCAGCGGA AGCTTCCAGA GGAACATGCC 
AGATTTTATG CTGCTGAAAT TTGCATCGCT CTTAATTTTC TGCATGAGAA GGGCATTATT 
TACCGGGATT TAAAACTGGA CAATGTACTA TTGGACCAGG ATGGCCATAT CAAACTTACA 
GACTATGGCA TGTGCAAGGA GGGAATCAGA CCAGGTGACA CCACCAGCAC ATTCTGTGGG 
ACGCCCAACT ACATCGCCCC AGAGATCCTG AGGGGAGAGG ATTATGGTTT CAGTGTGGAC 
TGGTGGGCTC TCGGCGTGCT CATGTTTGAG ATGATGGCGG GCCGATCGCC CTTCGATATC 
ATCACTGACA ATCCTGACAT GAACACAGAG GAGTATCTCT TCCAGGTCAT TTTGGAGAAG 
CCGATTCGGA TTCCCAGGTC ATTGTCTGTT AAGGCTGCAA GTGTGCTGAA AGGTTTTCTG 
AACAAGGATC CGAAAGAGAG GCTGGGATGC CAAGTTCAAA CCGGATTCAC AGACATCAAG 
TCTCATACAT TCTTCAGAAG CATAGACTGG GACCAGCTGG AACAGAAGCA GGTGACGCCA 
CCGTTCAAAC CTCAAATCAC AGATGACTAC GGTTTAGAAA ACTTTGACAC ACAGTTCACC 
AACGAACCCG TGCAGCTCAC ACCAGATGAT GAGGATGTGA TAAAGAGAAT AGACCAGTCT 
GAGTTTGAGG GATTCGAGTA CATTAATCCT CTGCTGCTCT CCACTGAAGA AACCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcz ( XM_009303849.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302124.1 Danio rerio protein kinase C, zeta (prkcz), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa42228
Clone ID Related Accession (Same CDS sequence)	XM_009303849.2 , XM_009303849.3
Accession Version	XM_009303849.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein kinase C zeta type isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAG TCATGCCATC ACAGGAGCCG CTGGTAGACG ATGATAAAAG TGGAGAAGAA 
GTGGAGCTTC CCCCTGAGGA CCCAGAGGAC ACAGACGCAA TTCCAGTCCC TTTCTTAGCA 
CACAACCGAA AGGTGGAGAA AGCTGAAGAT GATTCAGAGG ACCTGAAGGC AGTGGTGGAT 
GGTATCGAGG GCATTCAGAT CTCTCAGTGT CTCGGTTTGG GGGATTTCGA CCTGATTCGA 
GTCATTGGCA GAGGCAGTTA CGCAAAAGTG CTGCTCGTCC GGCTCAAGAA GAACGAGCAG 
ATTTACGCCA TGAAGGTGGT GAAGAAAGAG CTGGTTCATG ATGATGAGAG GAAGAGAAAG 
GACATCGATT GGGTCCAGAC AGAAAAACAT GTGTTTGAAC AAGCATCGAC CAATCCATTT 
TTAGTGGGAC TTCACTCTTG CTTTCAGACA GAGAGCCGGC TCTTTCTAGT GATCGAGTAT 
GTGAATGGAG GAGATCTCAT GTTCCATATG CAGAGGCAGC GGAAGCTTCC AGAGGAACAT 
GCCAGATTTT ATGCTGCTGA AATTTGCATC GCTCTTAATT TTCTGCATGA GAAGGGCATT 
ATTTACCGGG ATTTAAAACT GGACAATGTA CTATTGGACC AGGATGGCCA TATCAAACTT 
ACAGACTATG GCATGTGCAA GGAGGGAATC AGACCAGGTG ACACCACCAG CACATTCTGT 
GGGACGCCCA ACTACATCGC CCCAGAGATC CTGAGGGGAG AGGATTATGG TTTCAGTGTG 
GACTGGTGGG CTCTCGGCGT GCTCATGTTT GAGATGATGG CGGGCCGATC GCCCTTCGAT 
ATCATCACTG ACAATCCTGA CATGAACACA GAGGAGTATC TCTTCCAGGT CATTTTGGAG 
AAGCCGATTC GGATTCCCAG GTCATTGTCT GTTAAGGCTG CAAGTGTGCT GAAAGGTTTT 
CTGAACAAGG ATCCGAAAGA GAGGCTGGGA TGCCAAGTTC AAACCGGATT CACAGACATC 
AAGTCTCATA CATTCTTCAG AAGCATAGAC TGGGACCAGC TGGAACAGAA GCAGGTGACG 
CCACCGTTCA AACCTCAAAT CACAGATGAC TACGGTTTAG AAAACTTTGA CACACAGTTC 
ACCAACGAAC CCGTGCAGCT CACACCAGAT GATGAGGATG TGATAAAGAG AATAGACCAG 
TCTGAGTTTG AGGGATTCGA GTACATTAAT CCTCTGCTGC TCTCCACTGA AGAAACCGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302124.1
CDS	299..1561
Translation	MDPVMPSQEPLVDDDKSGEEVELPPEDPEDTDAIPVPFLAHNRKVEKAEDDSEDLKAVVDGIEGIQISQCLGLGDFDLIRVIGRGSYAKVLLVRLKKNEQIYAMKVVKKELVHDDERKRKDIDWVQTEKHVFEQASTNPFLVGLHSCFQTESRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHEKGIIYRDLKLDNVLLDQDGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEEYLFQVILEKPIRIPRSLSVKAASVLKGFLNKDPKERLGCQVQTGFTDIKSHTFFRSIDWDQLEQKQVTPPFKPQITDDYGLENFDTQFTNEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV

Target ORF information:

RefSeq Version	XM_009303849.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase C, zeta (prkcz), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303849.2

ORF Insert Sequence:

ATGGATCCAG TCATGCCATC ACAGGAGCCG CTGGTAGACG ATGATAAAAG TGGAGAAGAA 
GTGGAGCTTC CCCCTGAGGA CCCAGAGGAC ACAGACGCAA TTCCAGTCCC TTTCTTAGCA 
CACAACCGAA AGGTGGAGAA AGCTGAAGAT GATTCAGAGG ACCTGAAGGC AGTGGTGGAT 
GGTATCGAGG GCATTCAGAT CTCTCAGTGT CTCGGTTTGG GGGATTTCGA CCTGATTCGA 
GTCATTGGCA GAGGCAGTTA CGCAAAAGTG CTGCTCGTCC GGCTCAAGAA GAACGAGCAG 
ATTTACGCCA TGAAGGTGGT GAAGAAAGAG CTGGTTCATG ATGATGAGAG GAAGAGAAAG 
GACATCGATT GGGTCCAGAC AGAAAAACAT GTGTTTGAAC AAGCATCGAC CAATCCATTT 
TTAGTGGGAC TTCACTCTTG CTTTCAGACA GAGAGCCGGC TCTTTCTAGT GATCGAGTAT 
GTGAATGGAG GAGATCTCAT GTTCCATATG CAGAGGCAGC GGAAGCTTCC AGAGGAACAT 
GCCAGATTTT ATGCTGCTGA AATTTGCATC GCTCTTAATT TTCTGCATGA GAAGGGCATT 
ATTTACCGGG ATTTAAAACT GGACAATGTA CTATTGGACC AGGATGGCCA TATCAAACTT 
ACAGACTATG GCATGTGCAA GGAGGGAATC AGACCAGGTG ACACCACCAG CACATTCTGT 
GGGACGCCCA ACTACATCGC CCCAGAGATC CTGAGGGGAG AGGATTATGG TTTCAGTGTG 
GACTGGTGGG CTCTCGGCGT GCTCATGTTT GAGATGATGG CGGGCCGATC GCCCTTCGAT 
ATCATCACTG ACAATCCTGA CATGAACACA GAGGAGTATC TCTTCCAGGT CATTTTGGAG 
AAGCCGATTC GGATTCCCAG GTCATTGTCT GTTAAGGCTG CAAGTGTGCT GAAAGGTTTT 
CTGAACAAGG ATCCGAAAGA GAGGCTGGGA TGCCAAGTTC AAACCGGATT CACAGACATC 
AAGTCTCATA CATTCTTCAG AAGCATAGAC TGGGACCAGC TGGAACAGAA GCAGGTGACG 
CCACCGTTCA AACCTCAAAT CACAGATGAC TACGGTTTAG AAAACTTTGA CACACAGTTC 
ACCAACGAAC CCGTGCAGCT CACACCAGAT GATGAGGATG TGATAAAGAG AATAGACCAG 
TCTGAGTTTG AGGGATTCGA GTACATTAAT CCTCTGCTGC TCTCCACTGA AGAAACCGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcz ( XM_009303849.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302124.1 Danio rerio protein kinase C, zeta (prkcz), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42228
Clone ID Related Accession (Same CDS sequence)	XM_009303849.2 , XM_009303849.3
Accession Version	XM_009303849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein kinase C zeta type isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAG TCATGCCATC ACAGGAGCCG CTGGTAGACG ATGATAAAAG TGGAGAAGAA 
GTGGAGCTTC CCCCTGAGGA CCCAGAGGAC ACAGACGCAA TTCCAGTCCC TTTCTTAGCA 
CACAACCGAA AGGTGGAGAA AGCTGAAGAT GATTCAGAGG ACCTGAAGGC AGTGGTGGAT 
GGTATCGAGG GCATTCAGAT CTCTCAGTGT CTCGGTTTGG GGGATTTCGA CCTGATTCGA 
GTCATTGGCA GAGGCAGTTA CGCAAAAGTG CTGCTCGTCC GGCTCAAGAA GAACGAGCAG 
ATTTACGCCA TGAAGGTGGT GAAGAAAGAG CTGGTTCATG ATGATGAGAG GAAGAGAAAG 
GACATCGATT GGGTCCAGAC AGAAAAACAT GTGTTTGAAC AAGCATCGAC CAATCCATTT 
TTAGTGGGAC TTCACTCTTG CTTTCAGACA GAGAGCCGGC TCTTTCTAGT GATCGAGTAT 
GTGAATGGAG GAGATCTCAT GTTCCATATG CAGAGGCAGC GGAAGCTTCC AGAGGAACAT 
GCCAGATTTT ATGCTGCTGA AATTTGCATC GCTCTTAATT TTCTGCATGA GAAGGGCATT 
ATTTACCGGG ATTTAAAACT GGACAATGTA CTATTGGACC AGGATGGCCA TATCAAACTT 
ACAGACTATG GCATGTGCAA GGAGGGAATC AGACCAGGTG ACACCACCAG CACATTCTGT 
GGGACGCCCA ACTACATCGC CCCAGAGATC CTGAGGGGAG AGGATTATGG TTTCAGTGTG 
GACTGGTGGG CTCTCGGCGT GCTCATGTTT GAGATGATGG CGGGCCGATC GCCCTTCGAT 
ATCATCACTG ACAATCCTGA CATGAACACA GAGGAGTATC TCTTCCAGGT CATTTTGGAG 
AAGCCGATTC GGATTCCCAG GTCATTGTCT GTTAAGGCTG CAAGTGTGCT GAAAGGTTTT 
CTGAACAAGG ATCCGAAAGA GAGGCTGGGA TGCCAAGTTC AAACCGGATT CACAGACATC 
AAGTCTCATA CATTCTTCAG AAGCATAGAC TGGGACCAGC TGGAACAGAA GCAGGTGACG 
CCACCGTTCA AACCTCAAAT CACAGATGAC TACGGTTTAG AAAACTTTGA CACACAGTTC 
ACCAACGAAC CCGTGCAGCT CACACCAGAT GATGAGGATG TGATAAAGAG AATAGACCAG 
TCTGAGTTTG AGGGATTCGA GTACATTAAT CCTCTGCTGC TCTCCACTGA AGAAACCGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302124.1
CDS	860..2122
Translation	MDPVMPSQEPLVDDDKSGEEVELPPEDPEDTDAIPVPFLAHNRKVEKAEDDSEDLKAVVDGIEGIQISQCLGLGDFDLIRVIGRGSYAKVLLVRLKKNEQIYAMKVVKKELVHDDERKRKDIDWVQTEKHVFEQASTNPFLVGLHSCFQTESRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHEKGIIYRDLKLDNVLLDQDGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEEYLFQVILEKPIRIPRSLSVKAASVLKGFLNKDPKERLGCQVQTGFTDIKSHTFFRSIDWDQLEQKQVTPPFKPQITDDYGLENFDTQFTNEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV

Target ORF information:

RefSeq Version	XM_009303849.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, zeta (prkcz), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303849.3

ORF Insert Sequence:

ATGGATCCAG TCATGCCATC ACAGGAGCCG CTGGTAGACG ATGATAAAAG TGGAGAAGAA 
GTGGAGCTTC CCCCTGAGGA CCCAGAGGAC ACAGACGCAA TTCCAGTCCC TTTCTTAGCA 
CACAACCGAA AGGTGGAGAA AGCTGAAGAT GATTCAGAGG ACCTGAAGGC AGTGGTGGAT 
GGTATCGAGG GCATTCAGAT CTCTCAGTGT CTCGGTTTGG GGGATTTCGA CCTGATTCGA 
GTCATTGGCA GAGGCAGTTA CGCAAAAGTG CTGCTCGTCC GGCTCAAGAA GAACGAGCAG 
ATTTACGCCA TGAAGGTGGT GAAGAAAGAG CTGGTTCATG ATGATGAGAG GAAGAGAAAG 
GACATCGATT GGGTCCAGAC AGAAAAACAT GTGTTTGAAC AAGCATCGAC CAATCCATTT 
TTAGTGGGAC TTCACTCTTG CTTTCAGACA GAGAGCCGGC TCTTTCTAGT GATCGAGTAT 
GTGAATGGAG GAGATCTCAT GTTCCATATG CAGAGGCAGC GGAAGCTTCC AGAGGAACAT 
GCCAGATTTT ATGCTGCTGA AATTTGCATC GCTCTTAATT TTCTGCATGA GAAGGGCATT 
ATTTACCGGG ATTTAAAACT GGACAATGTA CTATTGGACC AGGATGGCCA TATCAAACTT 
ACAGACTATG GCATGTGCAA GGAGGGAATC AGACCAGGTG ACACCACCAG CACATTCTGT 
GGGACGCCCA ACTACATCGC CCCAGAGATC CTGAGGGGAG AGGATTATGG TTTCAGTGTG 
GACTGGTGGG CTCTCGGCGT GCTCATGTTT GAGATGATGG CGGGCCGATC GCCCTTCGAT 
ATCATCACTG ACAATCCTGA CATGAACACA GAGGAGTATC TCTTCCAGGT CATTTTGGAG 
AAGCCGATTC GGATTCCCAG GTCATTGTCT GTTAAGGCTG CAAGTGTGCT GAAAGGTTTT 
CTGAACAAGG ATCCGAAAGA GAGGCTGGGA TGCCAAGTTC AAACCGGATT CACAGACATC 
AAGTCTCATA CATTCTTCAG AAGCATAGAC TGGGACCAGC TGGAACAGAA GCAGGTGACG 
CCACCGTTCA AACCTCAAAT CACAGATGAC TACGGTTTAG AAAACTTTGA CACACAGTTC 
ACCAACGAAC CCGTGCAGCT CACACCAGAT GATGAGGATG TGATAAAGAG AATAGACCAG 
TCTGAGTTTG AGGGATTCGA GTACATTAAT CCTCTGCTGC TCTCCACTGA AGAAACCGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Screen for Postembryonic Development in the Zebrafish (
Genetics207(2)609-623(2017 Oct)
Henke K,Daane JM,Hawkins MB,Dooley CM,Busch-Nentwich EM,Stemple DL,Harris MP

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes.
Proceedings of the National Academy of Sciences of the United States of America114(6)E941-E950(2017 Feb)
Ribas L,Liew WC,D?az N,Sreenivasan R,Orb?n L,Piferrer F

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta.
Developmental biology396(2)183-200(2014 Dec)
Gerlach GF,Wingert RA

The Par-PrkC polarity complex is required for cilia growth in zebrafish photoreceptors.
PloS one9(8)e104661(2014)
Krock BL,Perkins BD

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prkcz cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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