LOC555713 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC555713
Entrez Gene ID	555713
Full Name	neighbor of brca1 gene 1b
General protein information	Preferred Names neighbor of brca1 gene 1b Names next to BRCA1 gene 1 protein neighbor of BRCA1 gene 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299577.2	XP_009297852.2	next to BRCA1 gene 1 protein isoform X1
XM_021474335.1	XP_021330010.1	next to BRCA1 gene 1 protein isoform X1
XM_009299577.1	XP_009297852.1	next to BRCA1 gene 1 protein isoform X2
XM_001922956.4	XP_001922991.2	next to BRCA1 gene 1 protein isoform X2
XM_001922956.3	XP_001922991.1	next to BRCA1 gene 1 protein isoform X1

Pathways from BioSystems

Homologies

	NBR1	XP_001097043.2
Bos taurus (cattle)	NBR1	NP_001093837.1
Rattus norvegicus (Norway rat)	Nbr1	NP_001019936.1
Xenopus tropicalis (tropical clawed frog)	nbr1	XP_004918695.1
Homo sapiens (human)	NBR1	NP_114068.1
Mus musculus (house mouse)	Nbr1	NP_001239149.1
Gallus gallus (chicken)	NBR1	XP_418128.4
Canis lupus familiaris (dog)	NBR1	XP_537628.3
Danio rerio (zebrafish)	LOC555713	XP_001922991.1
Danio rerio (zebrafish)	nbr1	XP_005156369.1
Pan troglodytes (chimpanzee)	NBR1	XP_001155730.1

Gene Ontology

The following LOC555713 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC555713 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08523	XM_001922956.3 Latest version!	Danio rerio next to BRCA1 gene 1 protein (LOC555713), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa42220	XM_021474335.1 Latest version!	Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa42220	XM_009299577.1 Latest version!	Danio rerio next to BRCA1 gene 1 protein (LOC555713), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa146485	XM_001922956.4 Latest version!	Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa146486	XM_009299577.2 Latest version!	Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC555713 ( XM_001922956.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001922991.1 Danio rerio next to BRCA1 gene 1 protein (LOC555713), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08523
Clone ID Related Accession (Same CDS sequence)	XM_001922956.3
Accession Version	XM_001922956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	next to BRCA1 gene 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_001922956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTGA GTCGCAAAGT TCAGGCCGCA 
GTCCAAGAGG AAGGATTGGT AATTGTAAAT GAACTTAAAG GTGGGAAGGC AGAGGACAAG 
ACACCCCCAG CTTGGTTCAC GTCATACATG GAAAAGTTTA AGGACCAGGT GGTTCGAGAG 
GCAGTAGAGA AGATTTGTAG GGAGTTCTCT GGACAGTGCT GTATCCATAA GCCAGTAGGA 
GCAGAGGCTC AGGTCCCGGA GGTCACCTCA TCCACTCTTC CTGGGGCCCC AAGTTCAGCT 
CCAGCCTGCA GCAGCTGTCG GGGCCAGACC GCAGGCGGCG GATACCAATG CAGTGTGTGT 
ACATCCTGCA CTCTCTGTGA GCCTTGCAGC TTCTCACATG ATCCAAGCCA CAACCTTGTG 
AGAGCCAGAA CTCCTCTGTC TATTCCTGAA CACGGCTCGC CAGCACCAGA CCACAGCAGG 
TTCTACAGAA GAGGTGACCG GAGCTTTCGT AAGGCTGAGA AGCAGCGTCT CAAAGCGGAG 
AAACGTCAGC TGAAGGCTGA GGTAAAGGAG ATCAGAAAGC AGCTGAGGAT GGAGAGGCGA 
GGCCTACAGT GGAGCACTGC TGGAGAAGGA AACTCTTCAC CTGTCCTGCT GCAGCCACGA 
GCCACACAGG CAAACAGTCC TGATCGTCCT AAACGTCCAT GTCCACTAGC TGTGCCAGCC 
ATGACAGCTC TGCTGCTGGA TGAAAACCTG CCTGACGGGT CACGTCTGCG TCCTGGGACC 
AAGTTTATCA AATACTGGAA AATGAAGAAC AGTGGCAGAG TTTGCTGGGA CTCAGAGACC 
AAGTTGAAGT TCATGTGGGG GAACCTGGCA GTGGGCTCTG GAGAAAGATG GCGGGAGGTG 
CCAGTGCCCA CACTACAGCC AGGTCAGGTG GGTGTGGTCA GTGTGGCTCT TTGTGCTCCG 
ACGATAGAGG GCACTTACAC GTCCCATTGG CGCCTAGCTC ACAGAGGGGA GCAGTTTGGA 
CCACGTGTTT GGTGCAGCAT CGTTGTGGAT CCACATGCAC CTACAGCCAT CTGTGCAGAC 
GGGCTGCTGG TGTCTCCCTG TGTCACCCCA CAGGAGAAGT CTTCCCGCAC TCTTGAGAGG 
GAGGAGAAAT GTCGGGCCTC AGGGGACCAG TTACTTCTGT CCGTAAACCA AGACTGCGAT 
CAGGAATTCT ACATTCCATC TGTGGACTTA CTCACAGCTC AGGACTTACT GTCTTTCGAA 
CTGCTGGACA TCAACATTGT GCAAGAGCTG GAGAGCGTGC CCAACAATAC TCCTGCGGAC 
ACTACTCCAT GCATCTCACC TCTGCCCCAT GATGGACCAC TTCAGGAAAA ACCAACACTG 
GGACTCATCC AAGAGGAAAC TGAAGCCCAA GGTGTCAGCA ACATCCACGA TCAGGGTACA 
GAGTTGGAGG GAGTCCCAGC ACAAGAGAAG GGAGAGGAGA ACAGCATTGG GCCTCAGTTT 
GTCAGTCCAT CTGTTATCCG CTCACTCACC CTTGAAGTGG CAGAGCGTGG GGCACTTTGT 
GGAAGATACC CAGCTAAAGT GGTGCGCCCT GCACCCCAGG GTTCGATGTC ACTGTGCGAG 
AGACAATCAA AGAGCGTTTC AGAAACAGGA CTAATATCTG CTCCAGCTCC ACTGAAAGTA 
GAGGCACCTC TACTGTCTGA CCCCACCTCA CCACAGACAG AGACAACAGA GATCACTGTG 
CCCCTGCTAA CACTTCCAGC ACATCTTATC AGCACAGACG ATGGTCACGA GTCTGACATC 
GAGCAGATCC TGCTGGAGCC TGCAGGTGGA GATGCGGTAG AGGAAGGGGA GGAGGAAGTG 
GATAAGAAAG AGCAGCTGAA GGACCCCGAA GCACCCAAAG AAGTCCGCAG CAGAACTTCA 
TCAGCATCTT CAGAAGACTA CATCATCATC CTGCCTGACT GCTTCGACAC CAGTCGTCCG 
CTTGGAGAGT CCATGTACAG CTCAGCCCTG TCGCAGCCTG GAGATGAGCC TACTGAGGGG 
GAAAAACTGG AATCGGTGGA GACCCCAGAG CTCCAGAACC CTGCTGCTGC TGCTAGTGTG 
GATGCTAACG ACATGCTCTG TGCCTCCCAG ACTCTGGATG CTGTGCCGCT TACGCCTGTG 
ATTGTAGTGG CACCCCGACC CTCAGTCAGA TCCTGTGCAG AGACACAAGA GCAGATGGAT 
GATGGAGCAA AGGAGCAGGA GGGCAGTGAC CCGACAGAAG CAGGAGATAA AGAGAAAGAT 
TCTGAACCAA ATCAGCCTGA CTCCGAGAAC ACTTTAGCAT CAGCCAAGTC TGAAACAGAA 
GAGCTCAGGG CAAACAGCAT CACAGGTGGA TTAGTGAAGG GTGCCCTGTC TGTGGCAGCA 
TCTGCCTATA AAGCTCTTTT CACTGGACAG ACTGGCTCTG AAAAGCTCCC AGAGGATGCA 
GCTTCTCAAG ACGCCATGAT GGCGGTGCTG GTGGAGATGG GCTTCGGTGA TCGAGCTCTA 
AACCAACGTC TACTGCAGAA ACACAACCGC AACCTGCTGG ACGTGGTAAA CGAACTGGTG 
CAGATGACCG ACAACGACTG GTACACGACA CGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922991.1
CDS	138..3116
Translation	MDFYINLKVTFRGNAKSFLLSGSETKSWESMEAMVKRSFGLCNLQLTYFDEENEEVSVNSQLEYEEALKSAARQGNRLQMNVYETRGSRATVPGPGPPPGSGVGIAQVKPGTVGEPKKGFRPPQHCPTLAQVVSRKVQAAVQEEGLVIVNELKGGKAEDKTPPAWFTSYMEKFKDQVVREAVEKICREFSGQCCIHKPVGAEAQVPEVTSSTLPGAPSSAPACSSCRGQTAGGGYQCSVCTSCTLCEPCSFSHDPSHNLVRARTPLSIPEHGSPAPDHSRFYRRGDRSFRKAEKQRLKAEKRQLKAEVKEIRKQLRMERRGLQWSTAGEGNSSPVLLQPRATQANSPDRPKRPCPLAVPAMTALLLDENLPDGSRLRPGTKFIKYWKMKNSGRVCWDSETKLKFMWGNLAVGSGERWREVPVPTLQPGQVGVVSVALCAPTIEGTYTSHWRLAHRGEQFGPRVWCSIVVDPHAPTAICADGLLVSPCVTPQEKSSRTLEREEKCRASGDQLLLSVNQDCDQEFYIPSVDLLTAQDLLSFELLDINIVQELESVPNNTPADTTPCISPLPHDGPLQEKPTLGLIQEETEAQGVSNIHDQGTELEGVPAQEKGEENSIGPQFVSPSVIRSLTLEVAERGALCGRYPAKVVRPAPQGSMSLCERQSKSVSETGLISAPAPLKVEAPLLSDPTSPQTETTEITVPLLTLPAHLISTDDGHESDIEQILLEPAGGDAVEEGEEEVDKKEQLKDPEAPKEVRSRTSSASSEDYIIILPDCFDTSRPLGESMYSSALSQPGDEPTEGEKLESVETPELQNPAAAASVDANDMLCASQTLDAVPLTPVIVVAPRPSVRSCAETQEQMDDGAKEQEGSDPTEAGDKEKDSEPNQPDSENTLASAKSETEELRANSITGGLVKGALSVAASAYKALFTGQTGSEKLPEDAASQDAMMAVLVEMGFGDRALNQRLLQKHNRNLLDVVNELVQMTDNDWYTTRY

Target ORF information:

RefSeq Version	XM_001922956.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio next to BRCA1 gene 1 protein (LOC555713), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922956.3

ORF Insert Sequence:

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTGA GTCGCAAAGT TCAGGCCGCA 
GTCCAAGAGG AAGGATTGGT AATTGTAAAT GAACTTAAAG GTGGGAAGGC AGAGGACAAG 
ACACCCCCAG CTTGGTTCAC GTCATACATG GAAAAGTTTA AGGACCAGGT GGTTCGAGAG 
GCAGTAGAGA AGATTTGTAG GGAGTTCTCT GGACAGTGCT GTATCCATAA GCCAGTAGGA 
GCAGAGGCTC AGGTCCCGGA GGTCACCTCA TCCACTCTTC CTGGGGCCCC AAGTTCAGCT 
CCAGCCTGCA GCAGCTGTCG GGGCCAGACC GCAGGCGGCG GATACCAATG CAGTGTGTGT 
ACATCCTGCA CTCTCTGTGA GCCTTGCAGC TTCTCACATG ATCCAAGCCA CAACCTTGTG 
AGAGCCAGAA CTCCTCTGTC TATTCCTGAA CACGGCTCGC CAGCACCAGA CCACAGCAGG 
TTCTACAGAA GAGGTGACCG GAGCTTTCGT AAGGCTGAGA AGCAGCGTCT CAAAGCGGAG 
AAACGTCAGC TGAAGGCTGA GGTAAAGGAG ATCAGAAAGC AGCTGAGGAT GGAGAGGCGA 
GGCCTACAGT GGAGCACTGC TGGAGAAGGA AACTCTTCAC CTGTCCTGCT GCAGCCACGA 
GCCACACAGG CAAACAGTCC TGATCGTCCT AAACGTCCAT GTCCACTAGC TGTGCCAGCC 
ATGACAGCTC TGCTGCTGGA TGAAAACCTG CCTGACGGGT CACGTCTGCG TCCTGGGACC 
AAGTTTATCA AATACTGGAA AATGAAGAAC AGTGGCAGAG TTTGCTGGGA CTCAGAGACC 
AAGTTGAAGT TCATGTGGGG GAACCTGGCA GTGGGCTCTG GAGAAAGATG GCGGGAGGTG 
CCAGTGCCCA CACTACAGCC AGGTCAGGTG GGTGTGGTCA GTGTGGCTCT TTGTGCTCCG 
ACGATAGAGG GCACTTACAC GTCCCATTGG CGCCTAGCTC ACAGAGGGGA GCAGTTTGGA 
CCACGTGTTT GGTGCAGCAT CGTTGTGGAT CCACATGCAC CTACAGCCAT CTGTGCAGAC 
GGGCTGCTGG TGTCTCCCTG TGTCACCCCA CAGGAGAAGT CTTCCCGCAC TCTTGAGAGG 
GAGGAGAAAT GTCGGGCCTC AGGGGACCAG TTACTTCTGT CCGTAAACCA AGACTGCGAT 
CAGGAATTCT ACATTCCATC TGTGGACTTA CTCACAGCTC AGGACTTACT GTCTTTCGAA 
CTGCTGGACA TCAACATTGT GCAAGAGCTG GAGAGCGTGC CCAACAATAC TCCTGCGGAC 
ACTACTCCAT GCATCTCACC TCTGCCCCAT GATGGACCAC TTCAGGAAAA ACCAACACTG 
GGACTCATCC AAGAGGAAAC TGAAGCCCAA GGTGTCAGCA ACATCCACGA TCAGGGTACA 
GAGTTGGAGG GAGTCCCAGC ACAAGAGAAG GGAGAGGAGA ACAGCATTGG GCCTCAGTTT 
GTCAGTCCAT CTGTTATCCG CTCACTCACC CTTGAAGTGG CAGAGCGTGG GGCACTTTGT 
GGAAGATACC CAGCTAAAGT GGTGCGCCCT GCACCCCAGG GTTCGATGTC ACTGTGCGAG 
AGACAATCAA AGAGCGTTTC AGAAACAGGA CTAATATCTG CTCCAGCTCC ACTGAAAGTA 
GAGGCACCTC TACTGTCTGA CCCCACCTCA CCACAGACAG AGACAACAGA GATCACTGTG 
CCCCTGCTAA CACTTCCAGC ACATCTTATC AGCACAGACG ATGGTCACGA GTCTGACATC 
GAGCAGATCC TGCTGGAGCC TGCAGGTGGA GATGCGGTAG AGGAAGGGGA GGAGGAAGTG 
GATAAGAAAG AGCAGCTGAA GGACCCCGAA GCACCCAAAG AAGTCCGCAG CAGAACTTCA 
TCAGCATCTT CAGAAGACTA CATCATCATC CTGCCTGACT GCTTCGACAC CAGTCGTCCG 
CTTGGAGAGT CCATGTACAG CTCAGCCCTG TCGCAGCCTG GAGATGAGCC TACTGAGGGG 
GAAAAACTGG AATCGGTGGA GACCCCAGAG CTCCAGAACC CTGCTGCTGC TGCTAGTGTG 
GATGCTAACG ACATGCTCTG TGCCTCCCAG ACTCTGGATG CTGTGCCGCT TACGCCTGTG 
ATTGTAGTGG CACCCCGACC CTCAGTCAGA TCCTGTGCAG AGACACAAGA GCAGATGGAT 
GATGGAGCAA AGGAGCAGGA GGGCAGTGAC CCGACAGAAG CAGGAGATAA AGAGAAAGAT 
TCTGAACCAA ATCAGCCTGA CTCCGAGAAC ACTTTAGCAT CAGCCAAGTC TGAAACAGAA 
GAGCTCAGGG CAAACAGCAT CACAGGTGGA TTAGTGAAGG GTGCCCTGTC TGTGGCAGCA 
TCTGCCTATA AAGCTCTTTT CACTGGACAG ACTGGCTCTG AAAAGCTCCC AGAGGATGCA 
GCTTCTCAAG ACGCCATGAT GGCGGTGCTG GTGGAGATGG GCTTCGGTGA TCGAGCTCTA 
AACCAACGTC TACTGCAGAA ACACAACCGC AACCTGCTGG ACGTGGTAAA CGAACTGGTG 
CAGATGACCG ACAACGACTG GTACACGACA CGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC555713 ( XM_021474335.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021330010.1 Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42220
Clone ID Related Accession (Same CDS sequence)	XM_021474335.1 , XM_009299577.1
Accession Version	XM_021474335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	next to BRCA1 gene 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTAA TTGTAAATGA ACTTAAAGGT 
GGGAAGGCAG AGGACAAGAC ACCCCCAGCT TGGTTCACGT CATACATGGA AAAGTTTAAG 
GACCAGGTGG TTCGAGAGGC AGTAGAGAAG ATTTGTAGGG AGTTCTCTGG ACAGTGCTGT 
ATCCATAAGC CAGTAGGAGC AGAGGCTCAG GTCCCGGAGG TCACCTCATC CACTCTTCCT 
GGGGCCCCAA GTTCAGCTCC AGCCTGCAGC AGCTGTCGGG GCCAGACCGC AGGCGGCGGA 
TACCAATGCA GTGTGTGTAC ATCCTGCACT CTCTGTGAGC CTTGCAGCTT CTCACATGAT 
CCAAGCCACA ACCTTGTGAG AGCCAGAACT CCTCTGTCTA TTCCTGAACA CGGCTCGCCA 
GCACCAGACC ACAGCAGGTT CTACAGAAGA GGTGACCGGA GCTTTCGTAA GGCTGAGAAG 
CAGCGTCTCA AAGCGGAGAA ACGTCAGCTG AAGGCTGAGG TAAAGGAGAT CAGAAAGCAG 
CTGAGGATGG AGAGGCGAGG CCTACAGTGG AGCACTGCTG GAGAAGGAAA CTCTTCACCT 
GTCCTGCTGC AGCCACGAGC CACACAGGCA AACAGTCCTG ATCGTCCTAA ACGTCCATGT 
CCACTAGCTG TGCCAGCCAT GACAGCTCTG CTGCTGGATG AAAACCTGCC TGACGGGTCA 
CGTCTGCGTC CTGGGACCAA GTTTATCAAA TACTGGAAAA TGAAGAACAG TGGCAGAGTT 
TGCTGGGACT CAGAGACCAA GTTGAAGTTC ATGTGGGGGA ACCTGGCAGT GGGCTCTGGA 
GAAAGATGGC GGGAGGTGCC AGTGCCCACA CTACAGCCAG GTCAGGTGGG TGTGGTCAGT 
GTGGCTCTTT GTGCTCCGAC GATAGAGGGC ACTTACACGT CCCATTGGCG CCTAGCTCAC 
AGAGGGGAGC AGTTTGGACC ACGTGTTTGG TGCAGCATCG TTGTGGATCC ACATGCACCT 
ACAGCCATCT GTGCAGACGG GCTGCTGGTG TCTCCCTGTG TCACCCCACA GGAGAAGTCT 
TCCCGCACTC TTGAGAGGGA GGAGAAATGT CGGGCCTCAG GGGACCAGTT ACTTCTGTCC 
GTAAACCAAG ACTGCGATCA GGAATTCTAC ATTCCATCTG TGGACTTACT CACAGCTCAG 
GACTTACTGT CTTTCGAACT GCTGGACATC AACATTGTGC AAGAGCTGGA GAGCGTGCCC 
AACAATACTC CTGCGGACAC TACTCCATGC ATCTCACCTC TGCCCCATGA TGGACCACTT 
CAGGAAAAAC CAACACTGGG ACTCATCCAA GAGGAAACTG AAGCCCAAGG TGTCAGCAAC 
ATCCACGATC AGGGTACAGA GTTGGAGGGA GTCCCAGCAC AAGAGAAGGG AGAGGAGAAC 
AGCATTGGGC CTCAGTTTGT CAGTCCATCT GTTATCCGCT CACTCACCCT TGAAGTGGCA 
GAGCGTGGGG CACTTTGTGG AAGATACCCA GCTAAAGTGG TGCGCCCTGC ACCCCAGGGT 
TCGATGTCAC TGTGCGAGAG ACAATCAAAG AGCGTTTCAG AAACAGGACT AATATCTGCT 
CCAGCTCCAC TGAAAGTAGA GGCACCTCTA CTGTCTGACC CCACCTCACC ACAGACAGAG 
ACAACAGAGA TCACTGTGCC CCTGCTAACA CTTCCAGCAC ATCTTATCAG CACAGACGAT 
GGTCACGAGT CTGACATCGA GCAGATCCTG CTGGAGCCTG CAGGTGGAGA TGCGGTAGAG 
GAAGGGGAGG AGGAAGTGGA TAAGAAAGAG CAGCTGAAGG ACCCCGAAGC ACCCAAAGAA 
GTCCGCAGCA GAACTTCATC AGCATCTTCA GAAGACTACA TCATCATCCT GCCTGACTGC 
TTCGACACCA GTCGTCCGCT TGGAGAGTCC ATGTACAGCT CAGCCCTGTC GCAGCCTGGA 
GATGAGCCTA CTGAGGGGGA AAAACTGGAA TCGGTGGAGA CCCCAGAGCT CCAGAACCCT 
GCTGCTGCTG CTAGTGTGGA TGCTAACGAC ATGCTCTGTG CCTCCCAGAC TCTGGATGCT 
GTGCCGCTTA CGCCTGTGAT TGTAGTGGCA CCCCGACCCT CAGTCAGATC CTGTGCAGAG 
ACACAAGAGC AGATGGATGA TGGAGCAAAG GAGCAGGAGG GCAGTGACCC GACAGAAGCA 
GGAGATAAAG AGAAAGATTC TGAACCAAAT CAGCCTGACT CCGAGAACAC TTTAGCATCA 
GCCAAGTCTG AAACAGAAGA GCTCAGGGCA AACAGCATCA CAGGTGGATT AGTGAAGGGT 
GCCCTGTCTG TGGCAGCATC TGCCTATAAA GCTCTTTTCA CTGGACAGAC TGGCTCTGAA 
AAGCTCCCAG AGGATGCAGC TTCTCAAGAC GCCATGATGG CGGTGCTGGT GGAGATGGGC 
TTCGGTGATC GAGCTCTAAA CCAACGTCTA CTGCAGAAAC ACAACCGCAA CCTGCTGGAC 
GTGGTAAACG AACTGGTGCA GATGACCGAC AACGACTGGT ACACGACACG CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330010.1
CDS	113..3049
Translation	MDFYINLKVTFRGNAKSFLLSGSETKSWESMEAMVKRSFGLCNLQLTYFDEENEEVSVNSQLEYEEALKSAARQGNRLQMNVYETRGSRATVPGPGPPPGSGVGIAQVKPGTVGEPKKGFRPPQHCPTLAQVVIVNELKGGKAEDKTPPAWFTSYMEKFKDQVVREAVEKICREFSGQCCIHKPVGAEAQVPEVTSSTLPGAPSSAPACSSCRGQTAGGGYQCSVCTSCTLCEPCSFSHDPSHNLVRARTPLSIPEHGSPAPDHSRFYRRGDRSFRKAEKQRLKAEKRQLKAEVKEIRKQLRMERRGLQWSTAGEGNSSPVLLQPRATQANSPDRPKRPCPLAVPAMTALLLDENLPDGSRLRPGTKFIKYWKMKNSGRVCWDSETKLKFMWGNLAVGSGERWREVPVPTLQPGQVGVVSVALCAPTIEGTYTSHWRLAHRGEQFGPRVWCSIVVDPHAPTAICADGLLVSPCVTPQEKSSRTLEREEKCRASGDQLLLSVNQDCDQEFYIPSVDLLTAQDLLSFELLDINIVQELESVPNNTPADTTPCISPLPHDGPLQEKPTLGLIQEETEAQGVSNIHDQGTELEGVPAQEKGEENSIGPQFVSPSVIRSLTLEVAERGALCGRYPAKVVRPAPQGSMSLCERQSKSVSETGLISAPAPLKVEAPLLSDPTSPQTETTEITVPLLTLPAHLISTDDGHESDIEQILLEPAGGDAVEEGEEEVDKKEQLKDPEAPKEVRSRTSSASSEDYIIILPDCFDTSRPLGESMYSSALSQPGDEPTEGEKLESVETPELQNPAAAASVDANDMLCASQTLDAVPLTPVIVVAPRPSVRSCAETQEQMDDGAKEQEGSDPTEAGDKEKDSEPNQPDSENTLASAKSETEELRANSITGGLVKGALSVAASAYKALFTGQTGSEKLPEDAASQDAMMAVLVEMGFGDRALNQRLLQKHNRNLLDVVNELVQMTDNDWYTTRY

Target ORF information:

RefSeq Version	XM_021474335.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474335.1

ORF Insert Sequence:

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTAA TTGTAAATGA ACTTAAAGGT 
GGGAAGGCAG AGGACAAGAC ACCCCCAGCT TGGTTCACGT CATACATGGA AAAGTTTAAG 
GACCAGGTGG TTCGAGAGGC AGTAGAGAAG ATTTGTAGGG AGTTCTCTGG ACAGTGCTGT 
ATCCATAAGC CAGTAGGAGC AGAGGCTCAG GTCCCGGAGG TCACCTCATC CACTCTTCCT 
GGGGCCCCAA GTTCAGCTCC AGCCTGCAGC AGCTGTCGGG GCCAGACCGC AGGCGGCGGA 
TACCAATGCA GTGTGTGTAC ATCCTGCACT CTCTGTGAGC CTTGCAGCTT CTCACATGAT 
CCAAGCCACA ACCTTGTGAG AGCCAGAACT CCTCTGTCTA TTCCTGAACA CGGCTCGCCA 
GCACCAGACC ACAGCAGGTT CTACAGAAGA GGTGACCGGA GCTTTCGTAA GGCTGAGAAG 
CAGCGTCTCA AAGCGGAGAA ACGTCAGCTG AAGGCTGAGG TAAAGGAGAT CAGAAAGCAG 
CTGAGGATGG AGAGGCGAGG CCTACAGTGG AGCACTGCTG GAGAAGGAAA CTCTTCACCT 
GTCCTGCTGC AGCCACGAGC CACACAGGCA AACAGTCCTG ATCGTCCTAA ACGTCCATGT 
CCACTAGCTG TGCCAGCCAT GACAGCTCTG CTGCTGGATG AAAACCTGCC TGACGGGTCA 
CGTCTGCGTC CTGGGACCAA GTTTATCAAA TACTGGAAAA TGAAGAACAG TGGCAGAGTT 
TGCTGGGACT CAGAGACCAA GTTGAAGTTC ATGTGGGGGA ACCTGGCAGT GGGCTCTGGA 
GAAAGATGGC GGGAGGTGCC AGTGCCCACA CTACAGCCAG GTCAGGTGGG TGTGGTCAGT 
GTGGCTCTTT GTGCTCCGAC GATAGAGGGC ACTTACACGT CCCATTGGCG CCTAGCTCAC 
AGAGGGGAGC AGTTTGGACC ACGTGTTTGG TGCAGCATCG TTGTGGATCC ACATGCACCT 
ACAGCCATCT GTGCAGACGG GCTGCTGGTG TCTCCCTGTG TCACCCCACA GGAGAAGTCT 
TCCCGCACTC TTGAGAGGGA GGAGAAATGT CGGGCCTCAG GGGACCAGTT ACTTCTGTCC 
GTAAACCAAG ACTGCGATCA GGAATTCTAC ATTCCATCTG TGGACTTACT CACAGCTCAG 
GACTTACTGT CTTTCGAACT GCTGGACATC AACATTGTGC AAGAGCTGGA GAGCGTGCCC 
AACAATACTC CTGCGGACAC TACTCCATGC ATCTCACCTC TGCCCCATGA TGGACCACTT 
CAGGAAAAAC CAACACTGGG ACTCATCCAA GAGGAAACTG AAGCCCAAGG TGTCAGCAAC 
ATCCACGATC AGGGTACAGA GTTGGAGGGA GTCCCAGCAC AAGAGAAGGG AGAGGAGAAC 
AGCATTGGGC CTCAGTTTGT CAGTCCATCT GTTATCCGCT CACTCACCCT TGAAGTGGCA 
GAGCGTGGGG CACTTTGTGG AAGATACCCA GCTAAAGTGG TGCGCCCTGC ACCCCAGGGT 
TCGATGTCAC TGTGCGAGAG ACAATCAAAG AGCGTTTCAG AAACAGGACT AATATCTGCT 
CCAGCTCCAC TGAAAGTAGA GGCACCTCTA CTGTCTGACC CCACCTCACC ACAGACAGAG 
ACAACAGAGA TCACTGTGCC CCTGCTAACA CTTCCAGCAC ATCTTATCAG CACAGACGAT 
GGTCACGAGT CTGACATCGA GCAGATCCTG CTGGAGCCTG CAGGTGGAGA TGCGGTAGAG 
GAAGGGGAGG AGGAAGTGGA TAAGAAAGAG CAGCTGAAGG ACCCCGAAGC ACCCAAAGAA 
GTCCGCAGCA GAACTTCATC AGCATCTTCA GAAGACTACA TCATCATCCT GCCTGACTGC 
TTCGACACCA GTCGTCCGCT TGGAGAGTCC ATGTACAGCT CAGCCCTGTC GCAGCCTGGA 
GATGAGCCTA CTGAGGGGGA AAAACTGGAA TCGGTGGAGA CCCCAGAGCT CCAGAACCCT 
GCTGCTGCTG CTAGTGTGGA TGCTAACGAC ATGCTCTGTG CCTCCCAGAC TCTGGATGCT 
GTGCCGCTTA CGCCTGTGAT TGTAGTGGCA CCCCGACCCT CAGTCAGATC CTGTGCAGAG 
ACACAAGAGC AGATGGATGA TGGAGCAAAG GAGCAGGAGG GCAGTGACCC GACAGAAGCA 
GGAGATAAAG AGAAAGATTC TGAACCAAAT CAGCCTGACT CCGAGAACAC TTTAGCATCA 
GCCAAGTCTG AAACAGAAGA GCTCAGGGCA AACAGCATCA CAGGTGGATT AGTGAAGGGT 
GCCCTGTCTG TGGCAGCATC TGCCTATAAA GCTCTTTTCA CTGGACAGAC TGGCTCTGAA 
AAGCTCCCAG AGGATGCAGC TTCTCAAGAC GCCATGATGG CGGTGCTGGT GGAGATGGGC 
TTCGGTGATC GAGCTCTAAA CCAACGTCTA CTGCAGAAAC ACAACCGCAA CCTGCTGGAC 
GTGGTAAACG AACTGGTGCA GATGACCGAC AACGACTGGT ACACGACACG CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC555713 ( XM_009299577.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009297852.1 Danio rerio next to BRCA1 gene 1 protein (LOC555713), transcript variant X2, mRNA.

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CloneID	ODa42220
Clone ID Related Accession (Same CDS sequence)	XM_021474335.1 , XM_009299577.1
Accession Version	XM_009299577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	next to BRCA1 gene 1 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTAA TTGTAAATGA ACTTAAAGGT 
GGGAAGGCAG AGGACAAGAC ACCCCCAGCT TGGTTCACGT CATACATGGA AAAGTTTAAG 
GACCAGGTGG TTCGAGAGGC AGTAGAGAAG ATTTGTAGGG AGTTCTCTGG ACAGTGCTGT 
ATCCATAAGC CAGTAGGAGC AGAGGCTCAG GTCCCGGAGG TCACCTCATC CACTCTTCCT 
GGGGCCCCAA GTTCAGCTCC AGCCTGCAGC AGCTGTCGGG GCCAGACCGC AGGCGGCGGA 
TACCAATGCA GTGTGTGTAC ATCCTGCACT CTCTGTGAGC CTTGCAGCTT CTCACATGAT 
CCAAGCCACA ACCTTGTGAG AGCCAGAACT CCTCTGTCTA TTCCTGAACA CGGCTCGCCA 
GCACCAGACC ACAGCAGGTT CTACAGAAGA GGTGACCGGA GCTTTCGTAA GGCTGAGAAG 
CAGCGTCTCA AAGCGGAGAA ACGTCAGCTG AAGGCTGAGG TAAAGGAGAT CAGAAAGCAG 
CTGAGGATGG AGAGGCGAGG CCTACAGTGG AGCACTGCTG GAGAAGGAAA CTCTTCACCT 
GTCCTGCTGC AGCCACGAGC CACACAGGCA AACAGTCCTG ATCGTCCTAA ACGTCCATGT 
CCACTAGCTG TGCCAGCCAT GACAGCTCTG CTGCTGGATG AAAACCTGCC TGACGGGTCA 
CGTCTGCGTC CTGGGACCAA GTTTATCAAA TACTGGAAAA TGAAGAACAG TGGCAGAGTT 
TGCTGGGACT CAGAGACCAA GTTGAAGTTC ATGTGGGGGA ACCTGGCAGT GGGCTCTGGA 
GAAAGATGGC GGGAGGTGCC AGTGCCCACA CTACAGCCAG GTCAGGTGGG TGTGGTCAGT 
GTGGCTCTTT GTGCTCCGAC GATAGAGGGC ACTTACACGT CCCATTGGCG CCTAGCTCAC 
AGAGGGGAGC AGTTTGGACC ACGTGTTTGG TGCAGCATCG TTGTGGATCC ACATGCACCT 
ACAGCCATCT GTGCAGACGG GCTGCTGGTG TCTCCCTGTG TCACCCCACA GGAGAAGTCT 
TCCCGCACTC TTGAGAGGGA GGAGAAATGT CGGGCCTCAG GGGACCAGTT ACTTCTGTCC 
GTAAACCAAG ACTGCGATCA GGAATTCTAC ATTCCATCTG TGGACTTACT CACAGCTCAG 
GACTTACTGT CTTTCGAACT GCTGGACATC AACATTGTGC AAGAGCTGGA GAGCGTGCCC 
AACAATACTC CTGCGGACAC TACTCCATGC ATCTCACCTC TGCCCCATGA TGGACCACTT 
CAGGAAAAAC CAACACTGGG ACTCATCCAA GAGGAAACTG AAGCCCAAGG TGTCAGCAAC 
ATCCACGATC AGGGTACAGA GTTGGAGGGA GTCCCAGCAC AAGAGAAGGG AGAGGAGAAC 
AGCATTGGGC CTCAGTTTGT CAGTCCATCT GTTATCCGCT CACTCACCCT TGAAGTGGCA 
GAGCGTGGGG CACTTTGTGG AAGATACCCA GCTAAAGTGG TGCGCCCTGC ACCCCAGGGT 
TCGATGTCAC TGTGCGAGAG ACAATCAAAG AGCGTTTCAG AAACAGGACT AATATCTGCT 
CCAGCTCCAC TGAAAGTAGA GGCACCTCTA CTGTCTGACC CCACCTCACC ACAGACAGAG 
ACAACAGAGA TCACTGTGCC CCTGCTAACA CTTCCAGCAC ATCTTATCAG CACAGACGAT 
GGTCACGAGT CTGACATCGA GCAGATCCTG CTGGAGCCTG CAGGTGGAGA TGCGGTAGAG 
GAAGGGGAGG AGGAAGTGGA TAAGAAAGAG CAGCTGAAGG ACCCCGAAGC ACCCAAAGAA 
GTCCGCAGCA GAACTTCATC AGCATCTTCA GAAGACTACA TCATCATCCT GCCTGACTGC 
TTCGACACCA GTCGTCCGCT TGGAGAGTCC ATGTACAGCT CAGCCCTGTC GCAGCCTGGA 
GATGAGCCTA CTGAGGGGGA AAAACTGGAA TCGGTGGAGA CCCCAGAGCT CCAGAACCCT 
GCTGCTGCTG CTAGTGTGGA TGCTAACGAC ATGCTCTGTG CCTCCCAGAC TCTGGATGCT 
GTGCCGCTTA CGCCTGTGAT TGTAGTGGCA CCCCGACCCT CAGTCAGATC CTGTGCAGAG 
ACACAAGAGC AGATGGATGA TGGAGCAAAG GAGCAGGAGG GCAGTGACCC GACAGAAGCA 
GGAGATAAAG AGAAAGATTC TGAACCAAAT CAGCCTGACT CCGAGAACAC TTTAGCATCA 
GCCAAGTCTG AAACAGAAGA GCTCAGGGCA AACAGCATCA CAGGTGGATT AGTGAAGGGT 
GCCCTGTCTG TGGCAGCATC TGCCTATAAA GCTCTTTTCA CTGGACAGAC TGGCTCTGAA 
AAGCTCCCAG AGGATGCAGC TTCTCAAGAC GCCATGATGG CGGTGCTGGT GGAGATGGGC 
TTCGGTGATC GAGCTCTAAA CCAACGTCTA CTGCAGAAAC ACAACCGCAA CCTGCTGGAC 
GTGGTAAACG AACTGGTGCA GATGACCGAC AACGACTGGT ACACGACACG CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297852.1
CDS	138..3074
Translation	MDFYINLKVTFRGNAKSFLLSGSETKSWESMEAMVKRSFGLCNLQLTYFDEENEEVSVNSQLEYEEALKSAARQGNRLQMNVYETRGSRATVPGPGPPPGSGVGIAQVKPGTVGEPKKGFRPPQHCPTLAQVVIVNELKGGKAEDKTPPAWFTSYMEKFKDQVVREAVEKICREFSGQCCIHKPVGAEAQVPEVTSSTLPGAPSSAPACSSCRGQTAGGGYQCSVCTSCTLCEPCSFSHDPSHNLVRARTPLSIPEHGSPAPDHSRFYRRGDRSFRKAEKQRLKAEKRQLKAEVKEIRKQLRMERRGLQWSTAGEGNSSPVLLQPRATQANSPDRPKRPCPLAVPAMTALLLDENLPDGSRLRPGTKFIKYWKMKNSGRVCWDSETKLKFMWGNLAVGSGERWREVPVPTLQPGQVGVVSVALCAPTIEGTYTSHWRLAHRGEQFGPRVWCSIVVDPHAPTAICADGLLVSPCVTPQEKSSRTLEREEKCRASGDQLLLSVNQDCDQEFYIPSVDLLTAQDLLSFELLDINIVQELESVPNNTPADTTPCISPLPHDGPLQEKPTLGLIQEETEAQGVSNIHDQGTELEGVPAQEKGEENSIGPQFVSPSVIRSLTLEVAERGALCGRYPAKVVRPAPQGSMSLCERQSKSVSETGLISAPAPLKVEAPLLSDPTSPQTETTEITVPLLTLPAHLISTDDGHESDIEQILLEPAGGDAVEEGEEEVDKKEQLKDPEAPKEVRSRTSSASSEDYIIILPDCFDTSRPLGESMYSSALSQPGDEPTEGEKLESVETPELQNPAAAASVDANDMLCASQTLDAVPLTPVIVVAPRPSVRSCAETQEQMDDGAKEQEGSDPTEAGDKEKDSEPNQPDSENTLASAKSETEELRANSITGGLVKGALSVAASAYKALFTGQTGSEKLPEDAASQDAMMAVLVEMGFGDRALNQRLLQKHNRNLLDVVNELVQMTDNDWYTTRY

Target ORF information:

RefSeq Version	XM_009299577.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio next to BRCA1 gene 1 protein (LOC555713), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299577.1

ORF Insert Sequence:

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTAA TTGTAAATGA ACTTAAAGGT 
GGGAAGGCAG AGGACAAGAC ACCCCCAGCT TGGTTCACGT CATACATGGA AAAGTTTAAG 
GACCAGGTGG TTCGAGAGGC AGTAGAGAAG ATTTGTAGGG AGTTCTCTGG ACAGTGCTGT 
ATCCATAAGC CAGTAGGAGC AGAGGCTCAG GTCCCGGAGG TCACCTCATC CACTCTTCCT 
GGGGCCCCAA GTTCAGCTCC AGCCTGCAGC AGCTGTCGGG GCCAGACCGC AGGCGGCGGA 
TACCAATGCA GTGTGTGTAC ATCCTGCACT CTCTGTGAGC CTTGCAGCTT CTCACATGAT 
CCAAGCCACA ACCTTGTGAG AGCCAGAACT CCTCTGTCTA TTCCTGAACA CGGCTCGCCA 
GCACCAGACC ACAGCAGGTT CTACAGAAGA GGTGACCGGA GCTTTCGTAA GGCTGAGAAG 
CAGCGTCTCA AAGCGGAGAA ACGTCAGCTG AAGGCTGAGG TAAAGGAGAT CAGAAAGCAG 
CTGAGGATGG AGAGGCGAGG CCTACAGTGG AGCACTGCTG GAGAAGGAAA CTCTTCACCT 
GTCCTGCTGC AGCCACGAGC CACACAGGCA AACAGTCCTG ATCGTCCTAA ACGTCCATGT 
CCACTAGCTG TGCCAGCCAT GACAGCTCTG CTGCTGGATG AAAACCTGCC TGACGGGTCA 
CGTCTGCGTC CTGGGACCAA GTTTATCAAA TACTGGAAAA TGAAGAACAG TGGCAGAGTT 
TGCTGGGACT CAGAGACCAA GTTGAAGTTC ATGTGGGGGA ACCTGGCAGT GGGCTCTGGA 
GAAAGATGGC GGGAGGTGCC AGTGCCCACA CTACAGCCAG GTCAGGTGGG TGTGGTCAGT 
GTGGCTCTTT GTGCTCCGAC GATAGAGGGC ACTTACACGT CCCATTGGCG CCTAGCTCAC 
AGAGGGGAGC AGTTTGGACC ACGTGTTTGG TGCAGCATCG TTGTGGATCC ACATGCACCT 
ACAGCCATCT GTGCAGACGG GCTGCTGGTG TCTCCCTGTG TCACCCCACA GGAGAAGTCT 
TCCCGCACTC TTGAGAGGGA GGAGAAATGT CGGGCCTCAG GGGACCAGTT ACTTCTGTCC 
GTAAACCAAG ACTGCGATCA GGAATTCTAC ATTCCATCTG TGGACTTACT CACAGCTCAG 
GACTTACTGT CTTTCGAACT GCTGGACATC AACATTGTGC AAGAGCTGGA GAGCGTGCCC 
AACAATACTC CTGCGGACAC TACTCCATGC ATCTCACCTC TGCCCCATGA TGGACCACTT 
CAGGAAAAAC CAACACTGGG ACTCATCCAA GAGGAAACTG AAGCCCAAGG TGTCAGCAAC 
ATCCACGATC AGGGTACAGA GTTGGAGGGA GTCCCAGCAC AAGAGAAGGG AGAGGAGAAC 
AGCATTGGGC CTCAGTTTGT CAGTCCATCT GTTATCCGCT CACTCACCCT TGAAGTGGCA 
GAGCGTGGGG CACTTTGTGG AAGATACCCA GCTAAAGTGG TGCGCCCTGC ACCCCAGGGT 
TCGATGTCAC TGTGCGAGAG ACAATCAAAG AGCGTTTCAG AAACAGGACT AATATCTGCT 
CCAGCTCCAC TGAAAGTAGA GGCACCTCTA CTGTCTGACC CCACCTCACC ACAGACAGAG 
ACAACAGAGA TCACTGTGCC CCTGCTAACA CTTCCAGCAC ATCTTATCAG CACAGACGAT 
GGTCACGAGT CTGACATCGA GCAGATCCTG CTGGAGCCTG CAGGTGGAGA TGCGGTAGAG 
GAAGGGGAGG AGGAAGTGGA TAAGAAAGAG CAGCTGAAGG ACCCCGAAGC ACCCAAAGAA 
GTCCGCAGCA GAACTTCATC AGCATCTTCA GAAGACTACA TCATCATCCT GCCTGACTGC 
TTCGACACCA GTCGTCCGCT TGGAGAGTCC ATGTACAGCT CAGCCCTGTC GCAGCCTGGA 
GATGAGCCTA CTGAGGGGGA AAAACTGGAA TCGGTGGAGA CCCCAGAGCT CCAGAACCCT 
GCTGCTGCTG CTAGTGTGGA TGCTAACGAC ATGCTCTGTG CCTCCCAGAC TCTGGATGCT 
GTGCCGCTTA CGCCTGTGAT TGTAGTGGCA CCCCGACCCT CAGTCAGATC CTGTGCAGAG 
ACACAAGAGC AGATGGATGA TGGAGCAAAG GAGCAGGAGG GCAGTGACCC GACAGAAGCA 
GGAGATAAAG AGAAAGATTC TGAACCAAAT CAGCCTGACT CCGAGAACAC TTTAGCATCA 
GCCAAGTCTG AAACAGAAGA GCTCAGGGCA AACAGCATCA CAGGTGGATT AGTGAAGGGT 
GCCCTGTCTG TGGCAGCATC TGCCTATAAA GCTCTTTTCA CTGGACAGAC TGGCTCTGAA 
AAGCTCCCAG AGGATGCAGC TTCTCAAGAC GCCATGATGG CGGTGCTGGT GGAGATGGGC 
TTCGGTGATC GAGCTCTAAA CCAACGTCTA CTGCAGAAAC ACAACCGCAA CCTGCTGGAC 
GTGGTAAACG AACTGGTGCA GATGACCGAC AACGACTGGT ACACGACACG CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC555713 ( XM_001922956.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001922991.2 Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X2, mRNA.

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CloneID	ODa146485
Clone ID Related Accession (Same CDS sequence)	XM_001922956.4
Accession Version	XM_001922956.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	next to BRCA1 gene 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395227.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922956.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTGA GTCGCAAAGT TCAGGCCGCA 
GTCCAAGAGG AAGGATTGGT AATTGTAAAT GAACTTAAAG GTGGGAAGGC AGAGGACAAG 
ACACCCCCAG CTTGGTTCAC GTCATACATG GAAAAGTTTA AGGACCAGGT GGTTCGAGAG 
GCAGTAGAGA AGATTTGTAG GGAGTTCTCT GGACAGTGCT GTATCCATAA GCCAGTAGGA 
GCAGAGGCTC AGGTCCCGGA GGTCACCTCA TCCACTCTTC CTGGGGCCCC AAGTTCAGCT 
CCAGCCTGCA GCAGCTGTCG GGGCCAGACC GCAGGCGGCG GATACCAATG CAGTGTGTGT 
ACATCCTGCA CTCTCTGTGA GCCTTGCAGC TTCTCACATG ATCCAAGCCA CAACCTTGTG 
AGAGCCAGAA CTCCTCTGTC TATTCCTGAA CACGGCTCGC CAGCACCAGA CCACAGCAGG 
TTCTACAGAA GAGGTGACCG GAGCTTTCGT AAGGCTGAGA AGCAGCGTCT CAAAGCGGAG 
AAGCGTCAGC TGAAGGCTGA GGTAAAGGAG ATCAGAAAGC AGCTGAGGAT GGAGAGGCGA 
GGCCTGCAGT GGAGCACTGC TGGAGAAGGA AACTCTTCAC CTGTCCTGCT GCAGCCACGA 
GCCACACAGG CAAACAGTCC TGATCGTCCT AAACGTCCAT GTCCACTAGC TGTGCCAGCC 
ATGACAGCTC TGCTGCTGGA TGAAAACCTG CCTGACGGGT CACGTCTGCG TCCTGGGACC 
AAGTTTATCA AATACTGGAA AATGAAGAAC AGTGGCAGAG TTTGCTGGGA CTCAGAGACC 
AAGTTGAAGT TTATGTGGGG GAATCTGGCA GTGGGCTCTG GAGAAAGATG GCGGGAGGTG 
CCAGTGCCCA CACTACAGCC AGGTCAGGTG GGTGTGGTCA GTGTGGCTCT TTGTGCTCCG 
ACGATAGAGG GCACTTACAC GTCCCATTGG CGCCTAGCTC ACAGAGGGGA GCAGTTTGGA 
CCACGTGTTT GGTGCAGCAT CGTTGTGGAT CCACATGCAC CTACAGCCAT CTGTGCAGAC 
GGGCTGCTGG TGTCTCCCTG TGTCACCCCA CAGGAAAAGT CTTCCCGCAC TCTTGAGAGG 
GAGGAGAAAT GTCGGGCCTC AAGGGACCAG TTACTTCTGT CCGTAAACCA AGACTGCGAT 
CAGGAATTCT ACATTCCATC TGTGGACTTA CTCACAGCTC AGGACTTACT GTCTTTCGAA 
CTGCTGGACA TCAACATTGT GCAAGAGCTG GAGAGCGTGC CCAACAATAC TCCTGCGGAC 
ACTACTCCAT GCATCTCACC TCTGCCCCAT GATGGACCAC TTCAGGAAAA ACCAACACTG 
GGACTCATCC AAGAGGAAAC TGAAGCCCAA GGTGTCAGCA ACATCCTCGA TCAGGGTACA 
GAGTTGGAGG GAGTCCCAGC ACAAGAGAAG GGAGAGGAGA ACAGCATTGG GCCTCAGTTT 
GTCAGTCCAT CTGTTATCCG CTCACTCACC CTTGAAGTGG CAGAGCGTGG GGCACTTTGT 
GGAAGATACC CAGCTAAAGT GGTGCGCCCT GCACCCCAGG GTTCGATGTC ACTGTGTGAG 
AGAAAATCAA AGAGCGTTTC AGAAACAGGA CTAATATCTG CTCCAGCTCC ACTGAAAGTA 
GAGGCACCTC TACTGTCTGA CCCCACCTCA CCACAGACAG AGACAACAGA GATCACTGTG 
CCCCTGCTAA CACTTCCAGC ACATCTTATC AGCACAGACG ATGGTCACGA GTCTGACATC 
GAGCAGATCC TGCTGGAGCC TGCAGGTGGA GATGCGGTAG AGGAAGGGGA GGAGGAAGTG 
GATAAGAAAG AGCAGCTGAA GGACCCCGAA GCACCCAAAG AAGTCCGCAG CAGAACTTCA 
TCAGCATCTT CAGAAGACTA CATCATCATC CTGCCTGACT GCTTCGACAC CAGTCGTCCG 
CTTGGAGAGT CCATGTACAG CTCAGCCCTG TCGCAGCCTG GAGATGAGCC TACTGAGGGG 
GAAAAACTGG AATCGGTGGA GACCCCAGAG CTTCAGAACC CTGCTGCTGC TGCTAGTGTG 
GATGCTAACG ACATGCTCTG TGCCTCCCAG ACTCTGGATG CTGTGCCGCT TACGCCTGTG 
ATTGTAGTGG CACCCCGACC CTCAGTCAGA TCCTGTGCAG AGACACAAGA GCAGATGGAT 
GATGGAGCAA AGGAGCAGGA GGGCAGTGAC CCGACAGAAG CAGGAGATAA AGAGAAAGAT 
TCTGAACCAA ACCAGCCCGA CTCCGAGAAC ACTTTAGCAT CAGCCAAGTC TGAAACAGAA 
GAGCTCAGGG CAAACAGCAT CACAGGTGGA TTAGTGAAGG GTGCCCTGTC TGTGGCAGCA 
TCTGCCTATA AAGCTCTTTT CACTGGACAG ACTGGCTCTG AAAAGCTCCC AGAGGATGCA 
GCTTCTCAAG ACGCCATGAT GGCGGTGCTG GTGGAGATGG GCTTCGGTGA TCGAGCTCTA 
AACCAACGTC TACTGCAGAA ACACAACCGC AACCTGCTGG ACGTGGTAAA CGAACTGGTG 
CAGATGACCG ACAACGACTG GTACACGACA CGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922991.2
CDS	138..3116
Translation	MDFYINLKVTFRGNAKSFLLSGSETKSWESMEAMVKRSFGLCNLQLTYFDEENEEVSVNSQLEYEEALKSAARQGNRLQMNVYETRGSRATVPGPGPPPGSGVGIAQVKPGTVGEPKKGFRPPQHCPTLAQVVSRKVQAAVQEEGLVIVNELKGGKAEDKTPPAWFTSYMEKFKDQVVREAVEKICREFSGQCCIHKPVGAEAQVPEVTSSTLPGAPSSAPACSSCRGQTAGGGYQCSVCTSCTLCEPCSFSHDPSHNLVRARTPLSIPEHGSPAPDHSRFYRRGDRSFRKAEKQRLKAEKRQLKAEVKEIRKQLRMERRGLQWSTAGEGNSSPVLLQPRATQANSPDRPKRPCPLAVPAMTALLLDENLPDGSRLRPGTKFIKYWKMKNSGRVCWDSETKLKFMWGNLAVGSGERWREVPVPTLQPGQVGVVSVALCAPTIEGTYTSHWRLAHRGEQFGPRVWCSIVVDPHAPTAICADGLLVSPCVTPQEKSSRTLEREEKCRASRDQLLLSVNQDCDQEFYIPSVDLLTAQDLLSFELLDINIVQELESVPNNTPADTTPCISPLPHDGPLQEKPTLGLIQEETEAQGVSNILDQGTELEGVPAQEKGEENSIGPQFVSPSVIRSLTLEVAERGALCGRYPAKVVRPAPQGSMSLCERKSKSVSETGLISAPAPLKVEAPLLSDPTSPQTETTEITVPLLTLPAHLISTDDGHESDIEQILLEPAGGDAVEEGEEEVDKKEQLKDPEAPKEVRSRTSSASSEDYIIILPDCFDTSRPLGESMYSSALSQPGDEPTEGEKLESVETPELQNPAAAASVDANDMLCASQTLDAVPLTPVIVVAPRPSVRSCAETQEQMDDGAKEQEGSDPTEAGDKEKDSEPNQPDSENTLASAKSETEELRANSITGGLVKGALSVAASAYKALFTGQTGSEKLPEDAASQDAMMAVLVEMGFGDRALNQRLLQKHNRNLLDVVNELVQMTDNDWYTTRY

Target ORF information:

RefSeq Version	XM_001922956.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922956.4

ORF Insert Sequence:

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTGA GTCGCAAAGT TCAGGCCGCA 
GTCCAAGAGG AAGGATTGGT AATTGTAAAT GAACTTAAAG GTGGGAAGGC AGAGGACAAG 
ACACCCCCAG CTTGGTTCAC GTCATACATG GAAAAGTTTA AGGACCAGGT GGTTCGAGAG 
GCAGTAGAGA AGATTTGTAG GGAGTTCTCT GGACAGTGCT GTATCCATAA GCCAGTAGGA 
GCAGAGGCTC AGGTCCCGGA GGTCACCTCA TCCACTCTTC CTGGGGCCCC AAGTTCAGCT 
CCAGCCTGCA GCAGCTGTCG GGGCCAGACC GCAGGCGGCG GATACCAATG CAGTGTGTGT 
ACATCCTGCA CTCTCTGTGA GCCTTGCAGC TTCTCACATG ATCCAAGCCA CAACCTTGTG 
AGAGCCAGAA CTCCTCTGTC TATTCCTGAA CACGGCTCGC CAGCACCAGA CCACAGCAGG 
TTCTACAGAA GAGGTGACCG GAGCTTTCGT AAGGCTGAGA AGCAGCGTCT CAAAGCGGAG 
AAGCGTCAGC TGAAGGCTGA GGTAAAGGAG ATCAGAAAGC AGCTGAGGAT GGAGAGGCGA 
GGCCTGCAGT GGAGCACTGC TGGAGAAGGA AACTCTTCAC CTGTCCTGCT GCAGCCACGA 
GCCACACAGG CAAACAGTCC TGATCGTCCT AAACGTCCAT GTCCACTAGC TGTGCCAGCC 
ATGACAGCTC TGCTGCTGGA TGAAAACCTG CCTGACGGGT CACGTCTGCG TCCTGGGACC 
AAGTTTATCA AATACTGGAA AATGAAGAAC AGTGGCAGAG TTTGCTGGGA CTCAGAGACC 
AAGTTGAAGT TTATGTGGGG GAATCTGGCA GTGGGCTCTG GAGAAAGATG GCGGGAGGTG 
CCAGTGCCCA CACTACAGCC AGGTCAGGTG GGTGTGGTCA GTGTGGCTCT TTGTGCTCCG 
ACGATAGAGG GCACTTACAC GTCCCATTGG CGCCTAGCTC ACAGAGGGGA GCAGTTTGGA 
CCACGTGTTT GGTGCAGCAT CGTTGTGGAT CCACATGCAC CTACAGCCAT CTGTGCAGAC 
GGGCTGCTGG TGTCTCCCTG TGTCACCCCA CAGGAAAAGT CTTCCCGCAC TCTTGAGAGG 
GAGGAGAAAT GTCGGGCCTC AAGGGACCAG TTACTTCTGT CCGTAAACCA AGACTGCGAT 
CAGGAATTCT ACATTCCATC TGTGGACTTA CTCACAGCTC AGGACTTACT GTCTTTCGAA 
CTGCTGGACA TCAACATTGT GCAAGAGCTG GAGAGCGTGC CCAACAATAC TCCTGCGGAC 
ACTACTCCAT GCATCTCACC TCTGCCCCAT GATGGACCAC TTCAGGAAAA ACCAACACTG 
GGACTCATCC AAGAGGAAAC TGAAGCCCAA GGTGTCAGCA ACATCCTCGA TCAGGGTACA 
GAGTTGGAGG GAGTCCCAGC ACAAGAGAAG GGAGAGGAGA ACAGCATTGG GCCTCAGTTT 
GTCAGTCCAT CTGTTATCCG CTCACTCACC CTTGAAGTGG CAGAGCGTGG GGCACTTTGT 
GGAAGATACC CAGCTAAAGT GGTGCGCCCT GCACCCCAGG GTTCGATGTC ACTGTGTGAG 
AGAAAATCAA AGAGCGTTTC AGAAACAGGA CTAATATCTG CTCCAGCTCC ACTGAAAGTA 
GAGGCACCTC TACTGTCTGA CCCCACCTCA CCACAGACAG AGACAACAGA GATCACTGTG 
CCCCTGCTAA CACTTCCAGC ACATCTTATC AGCACAGACG ATGGTCACGA GTCTGACATC 
GAGCAGATCC TGCTGGAGCC TGCAGGTGGA GATGCGGTAG AGGAAGGGGA GGAGGAAGTG 
GATAAGAAAG AGCAGCTGAA GGACCCCGAA GCACCCAAAG AAGTCCGCAG CAGAACTTCA 
TCAGCATCTT CAGAAGACTA CATCATCATC CTGCCTGACT GCTTCGACAC CAGTCGTCCG 
CTTGGAGAGT CCATGTACAG CTCAGCCCTG TCGCAGCCTG GAGATGAGCC TACTGAGGGG 
GAAAAACTGG AATCGGTGGA GACCCCAGAG CTTCAGAACC CTGCTGCTGC TGCTAGTGTG 
GATGCTAACG ACATGCTCTG TGCCTCCCAG ACTCTGGATG CTGTGCCGCT TACGCCTGTG 
ATTGTAGTGG CACCCCGACC CTCAGTCAGA TCCTGTGCAG AGACACAAGA GCAGATGGAT 
GATGGAGCAA AGGAGCAGGA GGGCAGTGAC CCGACAGAAG CAGGAGATAA AGAGAAAGAT 
TCTGAACCAA ACCAGCCCGA CTCCGAGAAC ACTTTAGCAT CAGCCAAGTC TGAAACAGAA 
GAGCTCAGGG CAAACAGCAT CACAGGTGGA TTAGTGAAGG GTGCCCTGTC TGTGGCAGCA 
TCTGCCTATA AAGCTCTTTT CACTGGACAG ACTGGCTCTG AAAAGCTCCC AGAGGATGCA 
GCTTCTCAAG ACGCCATGAT GGCGGTGCTG GTGGAGATGG GCTTCGGTGA TCGAGCTCTA 
AACCAACGTC TACTGCAGAA ACACAACCGC AACCTGCTGG ACGTGGTAAA CGAACTGGTG 
CAGATGACCG ACAACGACTG GTACACGACA CGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC555713 ( XM_009299577.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009297852.2 Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X1, mRNA.

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CloneID	ODa146486
Clone ID Related Accession (Same CDS sequence)	XM_009299577.2
Accession Version	XM_009299577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	next to BRCA1 gene 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395227.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTAA TTGTAAATGA ACTTAAAGGT 
GGGAAGGCAG AGGACAAGAC ACCCCCAGCT TGGTTCACGT CATACATGGA AAAGTTTAAG 
GACCAGGTGG TTCGAGAGGC AGTAGAGAAG ATTTGTAGGG AGTTCTCTGG ACAGTGCTGT 
ATCCATAAGC CAGTAGGAGC AGAGGCTCAG GTCCCGGAGG TCACCTCATC CACTCTTCCT 
GGGGCCCCAA GTTCAGCTCC AGCCTGCAGC AGCTGTCGGG GCCAGACCGC AGGCGGCGGA 
TACCAATGCA GTGTGTGTAC ATCCTGCACT CTCTGTGAGC CTTGCAGCTT CTCACATGAT 
CCAAGCCACA ACCTTGTGAG AGCCAGAACT CCTCTGTCTA TTCCTGAACA CGGCTCGCCA 
GCACCAGACC ACAGCAGGTT CTACAGAAGA GGTGACCGGA GCTTTCGTAA GGCTGAGAAG 
CAGCGTCTCA AAGCGGAGAA GCGTCAGCTG AAGGCTGAGG TAAAGGAGAT CAGAAAGCAG 
CTGAGGATGG AGAGGCGAGG CCTGCAGTGG AGCACTGCTG GAGAAGGAAA CTCTTCACCT 
GTCCTGCTGC AGCCACGAGC CACACAGGCA AACAGTCCTG ATCGTCCTAA ACGTCCATGT 
CCACTAGCTG TGCCAGCCAT GACAGCTCTG CTGCTGGATG AAAACCTGCC TGACGGGTCA 
CGTCTGCGTC CTGGGACCAA GTTTATCAAA TACTGGAAAA TGAAGAACAG TGGCAGAGTT 
TGCTGGGACT CAGAGACCAA GTTGAAGTTT ATGTGGGGGA ATCTGGCAGT GGGCTCTGGA 
GAAAGATGGC GGGAGGTGCC AGTGCCCACA CTACAGCCAG GTCAGGTGGG TGTGGTCAGT 
GTGGCTCTTT GTGCTCCGAC GATAGAGGGC ACTTACACGT CCCATTGGCG CCTAGCTCAC 
AGAGGGGAGC AGTTTGGACC ACGTGTTTGG TGCAGCATCG TTGTGGATCC ACATGCACCT 
ACAGCCATCT GTGCAGACGG GCTGCTGGTG TCTCCCTGTG TCACCCCACA GGAAAAGTCT 
TCCCGCACTC TTGAGAGGGA GGAGAAATGT CGGGCCTCAA GGGACCAGTT ACTTCTGTCC 
GTAAACCAAG ACTGCGATCA GGAATTCTAC ATTCCATCTG TGGACTTACT CACAGCTCAG 
GACTTACTGT CTTTCGAACT GCTGGACATC AACATTGTGC AAGAGCTGGA GAGCGTGCCC 
AACAATACTC CTGCGGACAC TACTCCATGC ATCTCACCTC TGCCCCATGA TGGACCACTT 
CAGGAAAAAC CAACACTGGG ACTCATCCAA GAGGAAACTG AAGCCCAAGG TGTCAGCAAC 
ATCCTCGATC AGGGTACAGA GTTGGAGGGA GTCCCAGCAC AAGAGAAGGG AGAGGAGAAC 
AGCATTGGGC CTCAGTTTGT CAGTCCATCT GTTATCCGCT CACTCACCCT TGAAGTGGCA 
GAGCGTGGGG CACTTTGTGG AAGATACCCA GCTAAAGTGG TGCGCCCTGC ACCCCAGGGT 
TCGATGTCAC TGTGTGAGAG AAAATCAAAG AGCGTTTCAG AAACAGGACT AATATCTGCT 
CCAGCTCCAC TGAAAGTAGA GGCACCTCTA CTGTCTGACC CCACCTCACC ACAGACAGAG 
ACAACAGAGA TCACTGTGCC CCTGCTAACA CTTCCAGCAC ATCTTATCAG CACAGACGAT 
GGTCACGAGT CTGACATCGA GCAGATCCTG CTGGAGCCTG CAGGTGGAGA TGCGGTAGAG 
GAAGGGGAGG AGGAAGTGGA TAAGAAAGAG CAGCTGAAGG ACCCCGAAGC ACCCAAAGAA 
GTCCGCAGCA GAACTTCATC AGCATCTTCA GAAGACTACA TCATCATCCT GCCTGACTGC 
TTCGACACCA GTCGTCCGCT TGGAGAGTCC ATGTACAGCT CAGCCCTGTC GCAGCCTGGA 
GATGAGCCTA CTGAGGGGGA AAAACTGGAA TCGGTGGAGA CCCCAGAGCT TCAGAACCCT 
GCTGCTGCTG CTAGTGTGGA TGCTAACGAC ATGCTCTGTG CCTCCCAGAC TCTGGATGCT 
GTGCCGCTTA CGCCTGTGAT TGTAGTGGCA CCCCGACCCT CAGTCAGATC CTGTGCAGAG 
ACACAAGAGC AGATGGATGA TGGAGCAAAG GAGCAGGAGG GCAGTGACCC GACAGAAGCA 
GGAGATAAAG AGAAAGATTC TGAACCAAAC CAGCCCGACT CCGAGAACAC TTTAGCATCA 
GCCAAGTCTG AAACAGAAGA GCTCAGGGCA AACAGCATCA CAGGTGGATT AGTGAAGGGT 
GCCCTGTCTG TGGCAGCATC TGCCTATAAA GCTCTTTTCA CTGGACAGAC TGGCTCTGAA 
AAGCTCCCAG AGGATGCAGC TTCTCAAGAC GCCATGATGG CGGTGCTGGT GGAGATGGGC 
TTCGGTGATC GAGCTCTAAA CCAACGTCTA CTGCAGAAAC ACAACCGCAA CCTGCTGGAC 
GTGGTAAACG AACTGGTGCA GATGACCGAC AACGACTGGT ACACGACACG CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297852.2
CDS	138..3074
Translation	MDFYINLKVTFRGNAKSFLLSGSETKSWESMEAMVKRSFGLCNLQLTYFDEENEEVSVNSQLEYEEALKSAARQGNRLQMNVYETRGSRATVPGPGPPPGSGVGIAQVKPGTVGEPKKGFRPPQHCPTLAQVVIVNELKGGKAEDKTPPAWFTSYMEKFKDQVVREAVEKICREFSGQCCIHKPVGAEAQVPEVTSSTLPGAPSSAPACSSCRGQTAGGGYQCSVCTSCTLCEPCSFSHDPSHNLVRARTPLSIPEHGSPAPDHSRFYRRGDRSFRKAEKQRLKAEKRQLKAEVKEIRKQLRMERRGLQWSTAGEGNSSPVLLQPRATQANSPDRPKRPCPLAVPAMTALLLDENLPDGSRLRPGTKFIKYWKMKNSGRVCWDSETKLKFMWGNLAVGSGERWREVPVPTLQPGQVGVVSVALCAPTIEGTYTSHWRLAHRGEQFGPRVWCSIVVDPHAPTAICADGLLVSPCVTPQEKSSRTLEREEKCRASRDQLLLSVNQDCDQEFYIPSVDLLTAQDLLSFELLDINIVQELESVPNNTPADTTPCISPLPHDGPLQEKPTLGLIQEETEAQGVSNILDQGTELEGVPAQEKGEENSIGPQFVSPSVIRSLTLEVAERGALCGRYPAKVVRPAPQGSMSLCERKSKSVSETGLISAPAPLKVEAPLLSDPTSPQTETTEITVPLLTLPAHLISTDDGHESDIEQILLEPAGGDAVEEGEEEVDKKEQLKDPEAPKEVRSRTSSASSEDYIIILPDCFDTSRPLGESMYSSALSQPGDEPTEGEKLESVETPELQNPAAAASVDANDMLCASQTLDAVPLTPVIVVAPRPSVRSCAETQEQMDDGAKEQEGSDPTEAGDKEKDSEPNQPDSENTLASAKSETEELRANSITGGLVKGALSVAASAYKALFTGQTGSEKLPEDAASQDAMMAVLVEMGFGDRALNQRLLQKHNRNLLDVVNELVQMTDNDWYTTRY

Target ORF information:

RefSeq Version	XM_009299577.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neighbor of brca1 gene 1b (nbr1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299577.2

ORF Insert Sequence:

ATGGACTTTT ACATTAACCT AAAGGTGACT TTTCGAGGGA ATGCGAAGAG TTTCTTGCTG 
TCTGGCTCAG AGACGAAGAG TTGGGAGTCT ATGGAGGCCA TGGTGAAGAG GTCTTTTGGC 
CTGTGTAATT TACAACTAAC ATATTTCGAT GAGGAAAACG AAGAGGTATC AGTCAACAGT 
CAATTGGAAT ATGAAGAAGC CCTAAAGAGT GCTGCCAGAC AGGGGAACAG ACTACAAATG 
AATGTGTATG AGACAAGGGG CAGTAGAGCC ACAGTGCCAG GACCGGGCCC ACCACCAGGG 
TCAGGGGTGG GCATAGCCCA GGTGAAACCT GGTACTGTTG GAGAACCCAA GAAGGGATTC 
AGGCCTCCTC AGCACTGCCC AACTTTGGCA CAAGTGGTAA TTGTAAATGA ACTTAAAGGT 
GGGAAGGCAG AGGACAAGAC ACCCCCAGCT TGGTTCACGT CATACATGGA AAAGTTTAAG 
GACCAGGTGG TTCGAGAGGC AGTAGAGAAG ATTTGTAGGG AGTTCTCTGG ACAGTGCTGT 
ATCCATAAGC CAGTAGGAGC AGAGGCTCAG GTCCCGGAGG TCACCTCATC CACTCTTCCT 
GGGGCCCCAA GTTCAGCTCC AGCCTGCAGC AGCTGTCGGG GCCAGACCGC AGGCGGCGGA 
TACCAATGCA GTGTGTGTAC ATCCTGCACT CTCTGTGAGC CTTGCAGCTT CTCACATGAT 
CCAAGCCACA ACCTTGTGAG AGCCAGAACT CCTCTGTCTA TTCCTGAACA CGGCTCGCCA 
GCACCAGACC ACAGCAGGTT CTACAGAAGA GGTGACCGGA GCTTTCGTAA GGCTGAGAAG 
CAGCGTCTCA AAGCGGAGAA GCGTCAGCTG AAGGCTGAGG TAAAGGAGAT CAGAAAGCAG 
CTGAGGATGG AGAGGCGAGG CCTGCAGTGG AGCACTGCTG GAGAAGGAAA CTCTTCACCT 
GTCCTGCTGC AGCCACGAGC CACACAGGCA AACAGTCCTG ATCGTCCTAA ACGTCCATGT 
CCACTAGCTG TGCCAGCCAT GACAGCTCTG CTGCTGGATG AAAACCTGCC TGACGGGTCA 
CGTCTGCGTC CTGGGACCAA GTTTATCAAA TACTGGAAAA TGAAGAACAG TGGCAGAGTT 
TGCTGGGACT CAGAGACCAA GTTGAAGTTT ATGTGGGGGA ATCTGGCAGT GGGCTCTGGA 
GAAAGATGGC GGGAGGTGCC AGTGCCCACA CTACAGCCAG GTCAGGTGGG TGTGGTCAGT 
GTGGCTCTTT GTGCTCCGAC GATAGAGGGC ACTTACACGT CCCATTGGCG CCTAGCTCAC 
AGAGGGGAGC AGTTTGGACC ACGTGTTTGG TGCAGCATCG TTGTGGATCC ACATGCACCT 
ACAGCCATCT GTGCAGACGG GCTGCTGGTG TCTCCCTGTG TCACCCCACA GGAAAAGTCT 
TCCCGCACTC TTGAGAGGGA GGAGAAATGT CGGGCCTCAA GGGACCAGTT ACTTCTGTCC 
GTAAACCAAG ACTGCGATCA GGAATTCTAC ATTCCATCTG TGGACTTACT CACAGCTCAG 
GACTTACTGT CTTTCGAACT GCTGGACATC AACATTGTGC AAGAGCTGGA GAGCGTGCCC 
AACAATACTC CTGCGGACAC TACTCCATGC ATCTCACCTC TGCCCCATGA TGGACCACTT 
CAGGAAAAAC CAACACTGGG ACTCATCCAA GAGGAAACTG AAGCCCAAGG TGTCAGCAAC 
ATCCTCGATC AGGGTACAGA GTTGGAGGGA GTCCCAGCAC AAGAGAAGGG AGAGGAGAAC 
AGCATTGGGC CTCAGTTTGT CAGTCCATCT GTTATCCGCT CACTCACCCT TGAAGTGGCA 
GAGCGTGGGG CACTTTGTGG AAGATACCCA GCTAAAGTGG TGCGCCCTGC ACCCCAGGGT 
TCGATGTCAC TGTGTGAGAG AAAATCAAAG AGCGTTTCAG AAACAGGACT AATATCTGCT 
CCAGCTCCAC TGAAAGTAGA GGCACCTCTA CTGTCTGACC CCACCTCACC ACAGACAGAG 
ACAACAGAGA TCACTGTGCC CCTGCTAACA CTTCCAGCAC ATCTTATCAG CACAGACGAT 
GGTCACGAGT CTGACATCGA GCAGATCCTG CTGGAGCCTG CAGGTGGAGA TGCGGTAGAG 
GAAGGGGAGG AGGAAGTGGA TAAGAAAGAG CAGCTGAAGG ACCCCGAAGC ACCCAAAGAA 
GTCCGCAGCA GAACTTCATC AGCATCTTCA GAAGACTACA TCATCATCCT GCCTGACTGC 
TTCGACACCA GTCGTCCGCT TGGAGAGTCC ATGTACAGCT CAGCCCTGTC GCAGCCTGGA 
GATGAGCCTA CTGAGGGGGA AAAACTGGAA TCGGTGGAGA CCCCAGAGCT TCAGAACCCT 
GCTGCTGCTG CTAGTGTGGA TGCTAACGAC ATGCTCTGTG CCTCCCAGAC TCTGGATGCT 
GTGCCGCTTA CGCCTGTGAT TGTAGTGGCA CCCCGACCCT CAGTCAGATC CTGTGCAGAG 
ACACAAGAGC AGATGGATGA TGGAGCAAAG GAGCAGGAGG GCAGTGACCC GACAGAAGCA 
GGAGATAAAG AGAAAGATTC TGAACCAAAC CAGCCCGACT CCGAGAACAC TTTAGCATCA 
GCCAAGTCTG AAACAGAAGA GCTCAGGGCA AACAGCATCA CAGGTGGATT AGTGAAGGGT 
GCCCTGTCTG TGGCAGCATC TGCCTATAAA GCTCTTTTCA CTGGACAGAC TGGCTCTGAA 
AAGCTCCCAG AGGATGCAGC TTCTCAAGAC GCCATGATGG CGGTGCTGGT GGAGATGGGC 
TTCGGTGATC GAGCTCTAAA CCAACGTCTA CTGCAGAAAC ACAACCGCAA CCTGCTGGAC 
GTGGTAAACG AACTGGTGCA GATGACCGAC AACGACTGGT ACACGACACG CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC555713 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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