tmie cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmie
Entrez Gene ID	555430
Full Name	transmembrane inner ear
Synonyms	si:ch211-163f10.1
General protein information	Preferred Names transmembrane inner ear Names transmembrane inner ear expressed protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170647.1	NP_001164118.1	transmembrane inner ear expressed protein isoform a
XM_021480468.1	XP_021336143.1	transmembrane inner ear expressed protein isoform X1
NM_001170648.1	NP_001164119.1	transmembrane inner ear expressed protein isoform b

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt).
Erickson T, Morgan CP, Olt J, Hardy K, Busch-Nentwich E, Maeda R, Clemens R, Krey JF, Nechiporuk A, Barr-Gillespie PG, Marcotti W, Nicolson T
eLife6(2017 May)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

Gene miles-apart is required for formation of otic vesicle and hair cells in zebrafish.
Hu ZY, Zhang QY, Qin W, Tong JW, Zhao Q, Han Y, Meng J, Zhang JP
Cell death & disease4e900(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an early frameshift mutation in tmie discovered in a line of deaf and uncoordinated zebrafish with defective hair-cell function
Title: The transmembrane inner ear (Tmie) protein is essential for normal hearing and balance in the zebrafish.

Data show that transmembrane inner ear (tmie)appears to be required for inner ear development and function in the zebrafish and for hair cell maturation in the vestibular and lateral line systems as well.
Title: The transmembrane inner ear (tmie) gene contributes to vestibular and lateral line development and function in the zebrafish (Danio rerio).

The following tmie gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmie cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37324	NM_001170647.1 Latest version!	Danio rerio transmembrane inner ear (tmie), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa150323	XM_021480468.1 Latest version!	Danio rerio transmembrane inner ear (tmie), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
ODa37306	NM_001170648.1 Latest version!	Danio rerio transmembrane inner ear (tmie), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmie ( NM_001170647.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001164118.1 Danio rerio transmembrane inner ear (tmie), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa37324
Clone ID Related Accession (Same CDS sequence)	NM_001170647.1
Accession Version	NM_001170647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	transmembrane inner ear expressed protein isoform a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ389649.1. On Apr 26, 2010 this sequence version replaced XM_677930.3. ##Evidence-Data-START## Transcript exon combination :: GQ389649.1, EH440140.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGAGACGCG GGAGAAGAAG AGGGAAAATG GCGATGGAGG GGCCTGGGAA TCAGCCGCCA 
TCCCTGGTCC CTTTGCTGAT GAGCGTAACG GCGGTGTTGA TCTCACATCA TTTCTTTAAC 
GCGGCGGCGC AGATACCAGA CCCAGAGCTC CTGCCAACAG ACCCTCCGAA AAAGCCAGAC 
CCTGTCACCT CAGAAACAGT GGTGTTTTGG GGACTTCGAT TATGGCAGGT TGTGGGCATT 
TTCTCCATGT TCATCTTAGC AATAATAATA ACGCTCTGCT GCATCTTCAA ATGCCGAATC 
CCACGGACGA AAAAAGAGAT CGAGGCTCGG CACGCGCAAA GACTCGCTGC GAAAAAATAT 
GCCAACACAT TGGAGACGGT GCCTCCTCTC AATGAGCTGA CTGAGGTGCC CGGCGCTCTC 
GTGGAAACCT CAGCACTCCT GACGGTCTCA GAGCAGGTAC AGGCTCACGC TAGCGGACAA 
CTTCCTGCTG TCAGGGAAGA AGATGAACCG GCCAATCCTG GCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164118.1
CDS	1..528
Translation	MRRGRRRGKMAMEGPGNQPPSLVPLLMSVTAVLISHHFFNAAAQIPDPELLPTDPPKKPDPVTSETVVFWGLRLWQVVGIFSMFILAIIITLCCIFKCRIPRTKKEIEARHAQRLAAKKYANTLETVPPLNELTEVPGALVETSALLTVSEQVQAHASGQLPAVREEDEPANPGT

Target ORF information:

RefSeq Version	NM_001170647.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane inner ear (tmie), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170647.1

ORF Insert Sequence:

ATGAGACGCG GGAGAAGAAG AGGGAAAATG GCGATGGAGG GGCCTGGGAA TCAGCCGCCA 
TCCCTGGTCC CTTTGCTGAT GAGCGTAACG GCGGTGTTGA TCTCACATCA TTTCTTTAAC 
GCGGCGGCGC AGATACCAGA CCCAGAGCTC CTGCCAACAG ACCCTCCGAA AAAGCCAGAC 
CCTGTCACCT CAGAAACAGT GGTGTTTTGG GGACTTCGAT TATGGCAGGT TGTGGGCATT 
TTCTCCATGT TCATCTTAGC AATAATAATA ACGCTCTGCT GCATCTTCAA ATGCCGAATC 
CCACGGACGA AAAAAGAGAT CGAGGCTCGG CACGCGCAAA GACTCGCTGC GAAAAAATAT 
GCCAACACAT TGGAGACGGT GCCTCCTCTC AATGAGCTGA CTGAGGTGCC CGGCGCTCTC 
GTGGAAACCT CAGCACTCCT GACGGTCTCA GAGCAGGTAC AGGCTCACGC TAGCGGACAA 
CTTCCTGCTG TCAGGGAAGA AGATGAACCG GCCAATCCTG GCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmie ( XM_021480468.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336143.1 Danio rerio transmembrane inner ear (tmie), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150323
Clone ID Related Accession (Same CDS sequence)	XM_021480468.1
Accession Version	XM_021480468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane inner ear expressed protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACGCG GGAGAAGAAG AGGGAAAATG GCGATGGAGG GGCCTGGGAA TCAGCCGCCA 
TCCCTGGTCC CTTTGCTGAT GAGCGTAACG GCGGTGTTGA TCTCACATCA TTTCTTTAAC 
GCGGCGGCCC AGATACCAGA CCCAGAGCTC CTGCCAACAG ACCCTCCGAA AAAGCCAGAC 
CCCGTCACCT CAGAAACAGT GGTGTTTTGG GGACTTCGAT TATGGCAGGT TGTGGGCATT 
TTCTCCATGT TCATCTTAGC AATAACTCTC GTGGAAACCT CAGCACTCCT GACGGTCTCA 
GAGCAGGTAC AGGCTCACGC TAGCGGACAA CTTCCTGCTG TCAGGGAAGA AGATGAACCA 
GCCAATCCTG GCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336143.1
CDS	255..632
Translation	MRRGRRRGKMAMEGPGNQPPSLVPLLMSVTAVLISHHFFNAAAQIPDPELLPTDPPKKPDPVTSETVVFWGLRLWQVVGIFSMFILAITLVETSALLTVSEQVQAHASGQLPAVREEDEPANPGT

Target ORF information:

RefSeq Version	XM_021480468.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane inner ear (tmie), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480468.1

ORF Insert Sequence:

ATGAGACGCG GGAGAAGAAG AGGGAAAATG GCGATGGAGG GGCCTGGGAA TCAGCCGCCA 
TCCCTGGTCC CTTTGCTGAT GAGCGTAACG GCGGTGTTGA TCTCACATCA TTTCTTTAAC 
GCGGCGGCCC AGATACCAGA CCCAGAGCTC CTGCCAACAG ACCCTCCGAA AAAGCCAGAC 
CCCGTCACCT CAGAAACAGT GGTGTTTTGG GGACTTCGAT TATGGCAGGT TGTGGGCATT 
TTCTCCATGT TCATCTTAGC AATAACTCTC GTGGAAACCT CAGCACTCCT GACGGTCTCA 
GAGCAGGTAC AGGCTCACGC TAGCGGACAA CTTCCTGCTG TCAGGGAAGA AGATGAACCA 
GCCAATCCTG GCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmie ( NM_001170648.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001164119.1 Danio rerio transmembrane inner ear (tmie), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa37306
Clone ID Related Accession (Same CDS sequence)	NM_001170648.1
Accession Version	NM_001170648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	transmembrane inner ear expressed protein isoform b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ389648.1. ##Evidence-Data-START## Transcript exon combination :: GQ389648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505377, SAMEA3505384 [ECO:0000348] ##Evidence-Data-END##

ATGAGACGCG GGAGAAGAAG AGGGAAAATG GCGATGGAGG GGCCTGGGAA TCAGCCGCCA 
TCCCTGGTCC CTTTGCTGAT GAGCGTAACG GCGGTGTTGA TCTCACATCA TTTCTTTAAC 
GCGGCGGCGC AGATACCAGA CCCAGAGCTC CTGCCAACAG ACCCTCCGAA AAAGCCAGAC 
CCTGTCACCT CAGAAACAGT GGTGTTTTGG GGACTTCGAT TATGGCAGGT TGTGGGCATT 
TTCTCCATGT TCATCTTAGC AATAATAATA ACGCTCTGCT GCATCTTCAA ATGCCGAATC 
CCACGGACGA AAAAAGAGAT CGAGGCTCGG CACGCGCAAA GACTCGCTGC GAAAAAATAT 
GCCAACACAT TGGAGACGGT GCCTCCTCTC AATGAGCTGA CTGAGGTGCC CGGCGCGGCA 
AAGGTTGAGG TGAAGGAGGA GGTGCCAACC GTCGCAGGAA AGGTGGACGG AGAAAAGGAG 
AAAAAGAAGA AAGAAAAAGA GAGCAAAAAG GAAGGCAAAG GTGCAAAAGA AGGTAAAGAA 
GAGGAGAAAG AGGAACCCGC AAAAAAGAAA GGAGAAAAGG GAGAAAAAGG GGAGAAAGGA 
GCACCAAAGA AAGAAGCATC AGACGGAGGG AAAGGTAAAA AAGGAGGAGA AAAAGCAGAG 
GAGAAAGGAG GTGCCAAGAA GCCTGCGAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164119.1
CDS	1..696
Translation	MRRGRRRGKMAMEGPGNQPPSLVPLLMSVTAVLISHHFFNAAAQIPDPELLPTDPPKKPDPVTSETVVFWGLRLWQVVGIFSMFILAIIITLCCIFKCRIPRTKKEIEARHAQRLAAKKYANTLETVPPLNELTEVPGAAKVEVKEEVPTVAGKVDGEKEKKKKEKESKKEGKGAKEGKEEEKEEPAKKKGEKGEKGEKGAPKKEASDGGKGKKGGEKAEEKGGAKKPAKK

Target ORF information:

RefSeq Version	NM_001170648.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane inner ear (tmie), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170648.1

ORF Insert Sequence:

ATGAGACGCG GGAGAAGAAG AGGGAAAATG GCGATGGAGG GGCCTGGGAA TCAGCCGCCA 
TCCCTGGTCC CTTTGCTGAT GAGCGTAACG GCGGTGTTGA TCTCACATCA TTTCTTTAAC 
GCGGCGGCGC AGATACCAGA CCCAGAGCTC CTGCCAACAG ACCCTCCGAA AAAGCCAGAC 
CCTGTCACCT CAGAAACAGT GGTGTTTTGG GGACTTCGAT TATGGCAGGT TGTGGGCATT 
TTCTCCATGT TCATCTTAGC AATAATAATA ACGCTCTGCT GCATCTTCAA ATGCCGAATC 
CCACGGACGA AAAAAGAGAT CGAGGCTCGG CACGCGCAAA GACTCGCTGC GAAAAAATAT 
GCCAACACAT TGGAGACGGT GCCTCCTCTC AATGAGCTGA CTGAGGTGCC CGGCGCGGCA 
AAGGTTGAGG TGAAGGAGGA GGTGCCAACC GTCGCAGGAA AGGTGGACGG AGAAAAGGAG 
AAAAAGAAGA AAGAAAAAGA GAGCAAAAAG GAAGGCAAAG GTGCAAAAGA AGGTAAAGAA 
GAGGAGAAAG AGGAACCCGC AAAAAAGAAA GGAGAAAAGG GAGAAAAAGG GGAGAAAGGA 
GCACCAAAGA AAGAAGCATC AGACGGAGGG AAAGGTAAAA AAGGAGGAGA AAAAGCAGAG 
GAGAAAGGAG GTGCCAAGAA GCCTGCGAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt).
eLife6(2017 May)
Erickson T,Morgan CP,Olt J,Hardy K,Busch-Nentwich E,Maeda R,Clemens R,Krey JF,Nechiporuk A,Barr-Gillespie PG,Marcotti W,Nicolson T

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

Gene miles-apart is required for formation of otic vesicle and hair cells in zebrafish.
Cell death & disease4e900(2013 Oct)
Hu ZY,Zhang QY,Qin W,Tong JW,Zhao Q,Han Y,Meng J,Zhang JP

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

tmie cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報