cfap52 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cfap52
Entrez Gene ID	553666
Full Name	cilia and flagella associated protein 52
Synonyms	si:dkey-28k21.1,wdr16,wu:fj15f10,zgc:111988
General protein information	Preferred Names cilia and flagella associated protein 52 Names cilia- and flagella-associated protein 52 WD repeat domain 16 fj15f10
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020639.1	NP_001018475.1	cilia- and flagella-associated protein 52

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wdr16	NP_082239.2
Gallus gallus (chicken)	WDR16	XP_415591.2
Drosophila melanogaster (fruit fly)	CG10064	NP_648065.1
Xenopus tropicalis (tropical clawed frog)	wdr16	NP_001106545.1
Pan troglodytes (chimpanzee)	WDR16	XP_003315709.1
Bos taurus (cattle)	WDR16	XP_002695926.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005915	XP_315945.4
Homo sapiens (human)	WDR16	NP_659491.4
	WDR16	XP_001118238.2
Canis lupus familiaris (dog)	WDR16	XP_005620100.1
Rattus norvegicus (Norway rat)	Wdr16	NP_001094438.2
Danio rerio (zebrafish)	wdr16	NP_001018475.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Analysis of protein expression in zebrafish during gonad differentiation by targeted proteomics.
Groh KJ, Sch?nenberger R, Eggen RI, Segner H, Suter MJ
General and comparative endocrinology193210-20(2013 Nov)

Biosynthesis of Wdr16, a marker protein for kinocilia-bearing cells, starts at the time of kinocilia formation in rat, and wdr16 gene knockdown causes hydrocephalus in zebrafish.
Hirschner W, Pogoda HM, Kramer C, Thiess U, Hamprecht B, Wiesm?ller KH, Lautner M, Verleysdonk S
Journal of neurochemistry101(1)274-88(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wdr16 can be considered a differentiation marker of kinocilia-bearing cells. In the brain, it appears to be functionally related to water homeostasis or osmoregulation.(Wdr16, Danio Rerio)
Title: Biosynthesis of Wdr16, a marker protein for kinocilia-bearing cells, starts at the time of kinocilia formation in rat, and wdr16 gene knockdown causes hydrocephalus in zebrafish.

The following cfap52 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cfap52 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35788	NM_001020639.1 Latest version!	Danio rerio cilia and flagella associated protein 52 (cfap52), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cfap52 ( NM_001020639.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018475.1 Danio rerio cilia and flagella associated protein 52 (cfap52), mRNA.

Price & Availability↑

CloneID	ODa35788
Clone ID Related Accession (Same CDS sequence)	NM_001020639.1
Accession Version	NM_001020639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	cilia- and flagella-associated protein 52
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095635.1. ##Evidence-Data-START## Transcript exon combination :: BC095635.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAAG ACACACAAGA AGTCCCTCAA CTAGGATTAG AGGCTGTGAT AGGTTTTAAT 
GGACACGTGT TTTCTGGACT TAAAGTACAT CCAGACAAAG AACATCTCAT TTATCCTCTT 
GGCTGCACTG TAATTATTAA GAGTCTGAGA AGTGGAAAAC AAACTCTCCT CCATGGCCAC 
ACAAACAATG TCTCTTGCAT TTCTGTGTCT AAAAGTGGAC GCTATATTGC ATCTGGACAG 
GTTACTTTCA TGGGCTTTAA GGCTGATGTT ATTATCTGGG ACTATGAGAA GAAGGAGATT 
TATGCTCGTC TTTTGCTTCA TAAAGCTAAA GTTGAAGATC TCAGCTTCTC CCCAAATGAT 
AAATATTTGG TATCACTGGG TGGACAGGAT GATGCCAGTA TAGTAGTGTG GAACATCGAA 
AGTAAGGAGG CTATATGTGG CAGTCCTGCT TCAGCACAGA GCGCTGGTCA CTGCCTCGCC 
TTAGAGTACA CCAACTTGAG TGATGAAATC TTTGTCTCTG CTGGAAATGG AACTCTGCGT 
GTGTGGGAAC TAGATTTGCT TAACAGAAAG ATCCGGCCCA CAGAATGTCA GACAGGACAG 
CTCAAGAGAA TTGTCAAATG TCTTGAGATT CCAAATGATG ATAATTATTT CTACTGTGGA 
ACTACAAGTG GAGATATTCT GAAAGTGAAC CTGAAGACAA GGTTGCTCAG CAGCTGTGGC 
CCCGTTAAAC AAAAATTCAG CAAGGGAGTC AACACTCTAA AAGTCTTGAA GACTGGTGAC 
ATTTTAGTTG GATCAGGAGA CGGGATGTTG ACTTTGTGCT CAGGAGCCAA TTTCAAAACC 
ATTAAGAGTG TTCAGTTGGA AGGAGGGGTG ACATCAGTGA CCTTGCGTGG AGACGGACAC 
CAGTTCTATG TTGGCACAGA AGCAGCACAG ATGTACAGTT TAAGCTACAC TGACTTCAAA 
CCAGAGCTCA CTGCTACAAA CCACAACAGT GCAGTAAAGG ATGTGGCCTT TCCTTTTGGG 
ACATCAGAGC TCTTTGCCAC CTGTTCACAC AACGATATAC GAGTGTGGCA TTCTGAGTCG 
TCCAAGGAGC TTCTGCGTAT TACGGTGCCT AACATAACAT GCCATGCTCT TGGCTTCATG 
CGAGATGGAA GGAGCATCTT TAGTGCTTGG AATGATGGGA AGATTCGTGT GTTTACCCCA 
GAGAGTGGGA AGCTAAAGCT AATTATTCAT AATGCCCACA GTATGGCTGT AACTACTATA 
GCTGCGACCA ATGACTGCAA GAGGATCGTC AGTGGAGGAG GAGAAGGACA GGTGAGAGTT 
TGGGAGATAT TCCAAGACTC ATATCGACTC ATTGAGACTA TGAAAGAACA CAAAGCCACA 
GTGAACTGCA TTAAGATCAA GAGCAATGAC AAGGAGTGTG TGACGGCCAG CTCTGATGGA 
GCCTGCATCA TCTGGGACTT GGTGAGGTTT GTGAGGAATC AAATGGTCTT GTCCAACACC 
CTGTTCAGTG TTGTGTGTTA TCACCCTGAG GAATACCAGA TCATCACCAG TGGCACTGAC 
AGAAAGATTG GCTACTGGGA AGTATATGAT GGGTCTGCAA TCAGAGAACT CGAGGGCTCC 
TTGTCTGGAT CTATAAACGG CATGCATATT TCTGAAGATG GGAAATATTT TGTGACAGGT 
GGAGATGACA AACTACTCAA GCTCTGGCTC TATTCTGATG GTGAAGTGAC CCATGTTGGC 
ATCGGGCACA GCGGAAGCAT CACAAATGTG AGAATCTGTC CCAACAGCAG ATATATTGTC 
AGTACCAGTG CAGATGGTGC AATTCTAAGG TGGAGATACC CACAAACCGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018475.1
CDS	37..1890
Translation	MAEDTQEVPQLGLEAVIGFNGHVFSGLKVHPDKEHLIYPLGCTVIIKSLRSGKQTLLHGHTNNVSCISVSKSGRYIASGQVTFMGFKADVIIWDYEKKEIYARLLLHKAKVEDLSFSPNDKYLVSLGGQDDASIVVWNIESKEAICGSPASAQSAGHCLALEYTNLSDEIFVSAGNGTLRVWELDLLNRKIRPTECQTGQLKRIVKCLEIPNDDNYFYCGTTSGDILKVNLKTRLLSSCGPVKQKFSKGVNTLKVLKTGDILVGSGDGMLTLCSGANFKTIKSVQLEGGVTSVTLRGDGHQFYVGTEAAQMYSLSYTDFKPELTATNHNSAVKDVAFPFGTSELFATCSHNDIRVWHSESSKELLRITVPNITCHALGFMRDGRSIFSAWNDGKIRVFTPESGKLKLIIHNAHSMAVTTIAATNDCKRIVSGGGEGQVRVWEIFQDSYRLIETMKEHKATVNCIKIKSNDKECVTASSDGACIIWDLVRFVRNQMVLSNTLFSVVCYHPEEYQIITSGTDRKIGYWEVYDGSAIRELEGSLSGSINGMHISEDGKYFVTGGDDKLLKLWLYSDGEVTHVGIGHSGSITNVRICPNSRYIVSTSADGAILRWRYPQTA

Target ORF information:

RefSeq Version	NM_001020639.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cilia and flagella associated protein 52 (cfap52), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020639.1

ORF Insert Sequence:

ATGGCAGAAG ACACACAAGA AGTCCCTCAA CTAGGATTAG AGGCTGTGAT AGGTTTTAAT 
GGACACGTGT TTTCTGGACT TAAAGTACAT CCAGACAAAG AACATCTCAT TTATCCTCTT 
GGCTGCACTG TAATTATTAA GAGTCTGAGA AGTGGAAAAC AAACTCTCCT CCATGGCCAC 
ACAAACAATG TCTCTTGCAT TTCTGTGTCT AAAAGTGGAC GCTATATTGC ATCTGGACAG 
GTTACTTTCA TGGGCTTTAA GGCTGATGTT ATTATCTGGG ACTATGAGAA GAAGGAGATT 
TATGCTCGTC TTTTGCTTCA TAAAGCTAAA GTTGAAGATC TCAGCTTCTC CCCAAATGAT 
AAATATTTGG TATCACTGGG TGGACAGGAT GATGCCAGTA TAGTAGTGTG GAACATCGAA 
AGTAAGGAGG CTATATGTGG CAGTCCTGCT TCAGCACAGA GCGCTGGTCA CTGCCTCGCC 
TTAGAGTACA CCAACTTGAG TGATGAAATC TTTGTCTCTG CTGGAAATGG AACTCTGCGT 
GTGTGGGAAC TAGATTTGCT TAACAGAAAG ATCCGGCCCA CAGAATGTCA GACAGGACAG 
CTCAAGAGAA TTGTCAAATG TCTTGAGATT CCAAATGATG ATAATTATTT CTACTGTGGA 
ACTACAAGTG GAGATATTCT GAAAGTGAAC CTGAAGACAA GGTTGCTCAG CAGCTGTGGC 
CCCGTTAAAC AAAAATTCAG CAAGGGAGTC AACACTCTAA AAGTCTTGAA GACTGGTGAC 
ATTTTAGTTG GATCAGGAGA CGGGATGTTG ACTTTGTGCT CAGGAGCCAA TTTCAAAACC 
ATTAAGAGTG TTCAGTTGGA AGGAGGGGTG ACATCAGTGA CCTTGCGTGG AGACGGACAC 
CAGTTCTATG TTGGCACAGA AGCAGCACAG ATGTACAGTT TAAGCTACAC TGACTTCAAA 
CCAGAGCTCA CTGCTACAAA CCACAACAGT GCAGTAAAGG ATGTGGCCTT TCCTTTTGGG 
ACATCAGAGC TCTTTGCCAC CTGTTCACAC AACGATATAC GAGTGTGGCA TTCTGAGTCG 
TCCAAGGAGC TTCTGCGTAT TACGGTGCCT AACATAACAT GCCATGCTCT TGGCTTCATG 
CGAGATGGAA GGAGCATCTT TAGTGCTTGG AATGATGGGA AGATTCGTGT GTTTACCCCA 
GAGAGTGGGA AGCTAAAGCT AATTATTCAT AATGCCCACA GTATGGCTGT AACTACTATA 
GCTGCGACCA ATGACTGCAA GAGGATCGTC AGTGGAGGAG GAGAAGGACA GGTGAGAGTT 
TGGGAGATAT TCCAAGACTC ATATCGACTC ATTGAGACTA TGAAAGAACA CAAAGCCACA 
GTGAACTGCA TTAAGATCAA GAGCAATGAC AAGGAGTGTG TGACGGCCAG CTCTGATGGA 
GCCTGCATCA TCTGGGACTT GGTGAGGTTT GTGAGGAATC AAATGGTCTT GTCCAACACC 
CTGTTCAGTG TTGTGTGTTA TCACCCTGAG GAATACCAGA TCATCACCAG TGGCACTGAC 
AGAAAGATTG GCTACTGGGA AGTATATGAT GGGTCTGCAA TCAGAGAACT CGAGGGCTCC 
TTGTCTGGAT CTATAAACGG CATGCATATT TCTGAAGATG GGAAATATTT TGTGACAGGT 
GGAGATGACA AACTACTCAA GCTCTGGCTC TATTCTGATG GTGAAGTGAC CCATGTTGGC 
ATCGGGCACA GCGGAAGCAT CACAAATGTG AGAATCTGTC CCAACAGCAG ATATATTGTC 
AGTACCAGTG CAGATGGTGC AATTCTAAGG TGGAGATACC CACAAACCGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Analysis of protein expression in zebrafish during gonad differentiation by targeted proteomics.
General and comparative endocrinology193210-20(2013 Nov)
Groh KJ,Sch?nenberger R,Eggen RI,Segner H,Suter MJ

Biosynthesis of Wdr16, a marker protein for kinocilia-bearing cells, starts at the time of kinocilia formation in rat, and wdr16 gene knockdown causes hydrocephalus in zebrafish.
Journal of neurochemistry101(1)274-88(2007 Apr)
Hirschner W,Pogoda HM,Kramer C,Thiess U,Hamprecht B,Wiesm?ller KH,Lautner M,Verleysdonk S